LOCUS       DASTMD010000001         1902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_535784, whole genome shotgun sequence.
ACCESSION   DASTMD010000001 DASTMD010000000
VERSION     DASTMD010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535784"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..725
                     /locus_tag="VFQ54_00005"
     CDS             111..725
                     /locus_tag="VFQ54_00005"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU0163388.1"
                     /translation="MSVLLSGAGRTLENLLRVVARGELPIEIVSVISSVPDVRGLEVA
                     RDAGIPATTIVRKGHPSVASYSEAMYAALEPYAPDLILMAGFLRQMLVLPGWEGRILN
                     IHPALLPEASSYAAGKGLFGERVHQAVLAHGDRVTGATVHLVTDDYDQGPPLLRVEVP
                     VLEDDTSVTLGTRVFHAECDLYPEAIRRYMTANPHLRKSRSVSD"
     gene            complement(873..1184)
                     /locus_tag="VFQ54_00010"
     CDS             complement(873..1184)
                     /locus_tag="VFQ54_00010"
                     /inference="COORDINATES: protein motif:HMM:NF017320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HEU0163389.1"
                     /translation="MQVFGQTIDDQTRCVHWNSPLDVIAIRFACCGRYYPCYECHAEA
                     ESHPAQQWPRERWNELAILCGVCQTEITIATYKATNDCPACGAAFNPGCHLHAHLYFE
                     R"
     gene            complement(1193..1849)
                     /locus_tag="VFQ54_00015"
     CDS             complement(1193..1849)
                     /locus_tag="VFQ54_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163390.1"
                     /translation="MTPPETLTTAATADFNLAGLELIGPIARLQVQISPLKQGEKPHR
                     WYDPSPIASVPSLRIESGGVVGLDGDERRGDVHHRDHPLSRYRGENGISIGFTSHYAI
                     MRAHFGDHLTDGIAGENLLIETDRRLTGADLKGGVLVVSDRGVVAIDDIQVAPPCVEF
                     GKFSLRYGAERTADREVAAAVKFLHEGVRGFYATCQLDEDAIAALPPIAVGNLVYRRV
                     "
BASE COUNT          324 a          588 c          631 g          359 t
ORIGIN      
        1 gattggcgga ttttggggaa acggatacgc agcgtcgggg acgaaacatg tcgtttgagt
       61 cggggctacc ggtgggaatt cgcgaatcgg tcggtaagga acgatggcgg gtgtccgttc
      121 tcctttcggg agcggggcgg acgctcgaga acctgctacg cgtcgtcgcc cgcggcgaat
      181 tgccgatcga gatcgtctcc gtcatcagtt ccgtgccgga tgtgcgcggc ctcgaggttg
      241 ctcgcgacgc ggggatcccg gcgacgacga tcgtccgcaa gggccaccca tcagttgctt
      301 cctacagtga ggcgatgtat gcggcgctcg agccctacgc gcccgatctg atcctgatgg
      361 cggggttcct ccgccagatg ctggtgcttc ccggttggga aggtcgcatt ctcaacattc
      421 atccggcgct gctacccgag gcgtcctcgt atgccgccgg gaaggggctc ttcggcgagc
      481 gcgtccacca ggccgttctg gcacacggcg accgcgtaac cggcgcgacc gtccatctgg
      541 tgacggacga ttatgaccag gggccgccgc tgctccgtgt cgaggtccct gtcctggagg
      601 atgacacgtc cgtgacgctt ggcacgcgcg tcttccatgc cgaatgcgat ctgtacccgg
      661 aagcgatccg tcgctacatg acggccaatc cccatttgcg gaagagtcgc tcggtgagcg
      721 actgagctcg tcggctagag ataaactcta gccctacaaa cccggtcggt cgcaaacgtg
      781 tcgatcacgc gggctacgct gtcgatgtcg gccgttcccg cctcgcgctc gcccgaggat
      841 cgcgctcccc gtggcaacgg tttggatcgc cgctagcgtt cgaagtagag gtgggcgtga
      901 agatgacagc cggggttgaa ggccgcgccg caggctgggc aatcgtttgt cgccttgtag
      961 gtggcgatcg tgatctccgt ctggcagaca ccacagagga tcgccagttc gttccagcgc
     1021 tcgcgcggcc actgctgcgc gggatgtgac tccgcttcag cgtggcattc gtagcacgga
     1081 tagtagcgac cgcagcaagc gaacctgatc gcaatcacgt cgagcggcga gttccagtgc
     1141 acgcaccggg tctggtcgtc gatcgtctgg ccgaaaacct gcatcgctga acctagacgc
     1201 ggcggtagac gagattgccg acggcaattg gcgggagcgc ggcaatcgca tcctcgtcga
     1261 gctggcaggt cgcgtagaaa cctcgcaccc cctcgtggag gaacttcacg gctgccgcga
     1321 cctcccggtc cgccgtgcgc tcggcgccat agcgaagcga gaacttgccg aactcgacac
     1381 acggtggcgc gacctggatg tcgtcgatgg cgaccactcc gcgatcggag acgaccagga
     1441 caccaccctt gagatccgct ccggtaagcc gtcgatcggt ctcgatcagg aggttctcgc
     1501 cggcgattcc atccgtcagg tgatcgccga agtgcgcgcg catgatcgcg tagtgcgagg
     1561 tgaacccgat cgagatgccg ttctcgccgc gatatcgcga gagcggatga tcgcgatgat
     1621 ggacgtcacc ccgtcgctcg tccccgtcca gaccgacgac tccacctgat tcgatcctca
     1681 gcgacggcac gctcgcgatc ggcgatggat cgtaccagcg gtgaggcttc tcgccctgct
     1741 tcagcggcga gatctgaacc tggagccgtg cgatcggacc gatcaactcg agcccggcga
     1801 ggttgaaatc tgcggttgcg gcggtggtga gcgtttcggg aggggtcatg cggatgctcc
     1861 tttggggcaa ggcattgcgc gtgtgcgact gcacgattcg ct
//
LOCUS       DASTMD010000002         4722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1964156, whole genome shotgun sequence.
ACCESSION   DASTMD010000002 DASTMD010000000
VERSION     DASTMD010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964156"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFQ54_00020"
     CDS             <1..622
                     /locus_tag="VFQ54_00020"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU0163391.1"
                     /translation="AGVLSFDQALTLVRERGRLMKEAGTERPGGMAAVLGLTEEQLES
                     VCAEASSEGIINIANANCPGQTVISGEIQALLHAMELAKNAGARRVARLGVSIASHSP
                     LMSKVSQQLGDLVGRIPMRAPQVPIIGNVAGQAITTVDGIRRELAHHVERPVNWTRSV
                     REMIDGGATTFVELGAGQVLSGLIKRINKDVKTVGFRDLELGQQAV"
     gene            816..2048
                     /gene="fabF"
                     /locus_tag="VFQ54_00025"
     CDS             816..2048
                     /gene="fabF"
                     /locus_tag="VFQ54_00025"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU0163392.1"
                     /translation="MTRVVVTGVGAMTPLGIGVDTFWEGLTAGRSGIRRIQNFDPSDL
                     EVQIAGEVPDFVAKDHMDAKAARRMDRFAQFAVAASREALTNARFEITDDNRERVGIV
                     MNTGGGGIPTIENDVNVMREKGGRRVSPFLIPMFAPNMASCQVSIQFNIWGPTTTAAA
                     ACAAGVQAMIDAVFMIERGDADVVIAGGTEGGITPVAIASLTNMGALSKNNAHPDQAS
                     RPFDARRDGFVFSEGCAAFILESEEHAIARGADVICYASGGAYTADAYHITAGRPDGH
                     GQAQAMKLAMKRAAIEPKDIDYVAAHATATKIGDQGETVSVKKALGDHAYKVALSANK
                     SMVGHMLGAAGAVSALACVLAIRDGVIPPTINLMTPDPDCDLDYTPNVSRKLDVKVAM
                     ANGFGFGGQNASAVFKAV"
     gene            complement(2189..3631)
                     /locus_tag="VFQ54_00030"
     CDS             complement(2189..3631)
                     /locus_tag="VFQ54_00030"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HEU0163393.1"
                     /translation="MDNISSDGATEPRGKTLLIRNATLVATMDDDSRELPDTSLLIEG
                     GVIRQIGPASELPTTADDAIDARGMVILPGLVNTHHHFYQTLTRAIPAAQNAVLFDWL
                     KTLYPIWGGMTPEAIQVSTKVALTELLLSGCTTASDHTYLWPNGSRIDDQIEAAEEIG
                     VRFHASRGSMSVGESKGGLPPDSVVEDEDAILRDSRRAIETYHDASDGAMTRIVLAPC
                     SPFSVSPDLMRESVVLARSYGVHSHTHLAETRDEDDYCRKTFGRTPVELCEDLGWVGE
                     DVWHAHLVHPAADEITRLGASQTGAAHCPTSNMRLGSGIPRVGAWLRAGMRVGLGVDG
                     SASNDGSHLLAEARQAMLLQRVADGGDPLAMSAREALRIATRGGAEVLGRTDVGYLAP
                     GMRGDLIGIRVDTLPFAGGAVHDPLAAIVFCQPGNVDLSVIEGRVRVRDGQIVDVDLP
                     SIVARHNEIAVALARGELATQRFFSPRGGT"
     gene            complement(3813..>4722)
                     /locus_tag="VFQ54_00035"
     CDS             complement(3813..>4722)
                     /locus_tag="VFQ54_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0163394.1"
                     /translation="TTTAMEVVADVDLRGKRAIVTGGASGIGLETARALASAGAEVTI
                     GVRNLEAGMKAAAEIAPAVGEGRMLVMPLDLADRASIAAFVAFWEDPLDILVNNAGIM
                     ASPEMRTAEGWELQFATNHLGHFALALGLHGSLAAAHRARVVVVSSVGHVNGEVLFDD
                     IQFERHPYDAWQAYSQSKTANVLFMVEAARRWHEDGIAVNVLNPGRIVGTGLSRYIGD
                     VANSPSTFDPTSTDVSWKNAEQGAATSVLLAASPLVEGVTGRYFEDCNEAVPYVAPVR
                     RGVAAYALDPGKAARLWQVSLDLLAR"
BASE COUNT          841 a         1597 c         1519 g          765 t
ORIGIN      
        1 cgccggcgtc ctcagcttcg accaggcgct caccctcgtt cgcgagcggg gccgtctgat
       61 gaaggaagcc ggcaccgagc ggccgggcgg catggccgcc gttctcggcc tgaccgaaga
      121 gcagctggag tccgtctgcg ccgaggcatc gagcgagggc atcatcaata tcgccaacgc
      181 caactgcccg ggacagaccg tgatctccgg cgagatccag gcgcttctcc atgcgatgga
      241 gctcgcgaag aacgccggcg ctcgccgcgt cgcccgcctc ggtgtcagca tcgcctcgca
      301 ttcgccattg atgtcgaagg tctcgcagca actcggcgat ctcgtcggca ggattccgat
      361 gcgggcaccg caggtgccga tcatcggaaa cgtggccggt caggcgatca ccaccgtcga
      421 cggcatccgc cgcgagctcg cccatcacgt cgaacgcccg gtcaactgga cacggtccgt
      481 tcgcgagatg atcgatggcg gggcgacgac gttcgtcgaa ctcggcgccg ggcaggtcct
      541 ctccgggttg atcaagcgga tcaacaagga cgtgaagacg gtcggcttcc gcgatctcga
      601 actcggtcag caggcggtct aggcatcgat cggttgggca ctcccgggga ttcccattag
      661 aatgacgcgg caatgccgct tatcatgcat cgcgcgccga cccgagcgtc ggtacgcatg
      721 aggatcgtgc gccgccattg gcacgggggc atgacccgat ttcaaacgct cataagcaac
      781 tgcgaacgca atccggcgaa gaaaggactg agatcgtgac tcgagtggtc gtaacggggg
      841 tgggggcaat gactccactc gggatcggcg tcgatacctt ctgggaaggg ctgacggctg
      901 gtcggtccgg cattcgccgg atccagaact tcgacccgag tgatcttgag gtccagatcg
      961 ctggtgaggt gccggacttc gtcgccaagg accacatgga tgcgaaggcc gcccgccgga
     1021 tggatcgctt cgcgcagttc gccgtcgccg cctctcgcga ggcgctcacg aatgccaggt
     1081 tcgaaatcac cgacgacaat cgcgagcgcg tcggcatcgt gatgaacacc ggcggcggcg
     1141 gtatcccgac gatcgagaac gatgtcaacg tgatgcggga gaagggtggc cgtcgcgtca
     1201 gccccttcct gatcccgatg ttcgccccga acatggccag ttgccaggtc tcgatccagt
     1261 tcaacatctg gggtccgacc acgacggcag ccgcggcctg cgccgccgga gtccaggcga
     1321 tgatcgatgc cgtcttcatg atcgagcgcg gtgatgccga tgtcgtgatc gccggcggca
     1381 ccgagggcgg gatcacgccg gtcgcgatcg ccagcctcac caacatgggt gcgctctcca
     1441 agaacaacgc acatccggac caggcgagcc gcccattcga tgccaggcga gatggcttcg
     1501 tcttcagcga gggctgcgcc gccttcatcc tggaatcgga ggagcacgcg atcgcccgcg
     1561 gcgcggacgt gatctgctac gcatccggcg gcgcctacac cgccgacgcc tatcacatca
     1621 ccgccggtcg tccggacggt catgggcagg cccaggccat gaaactggcg atgaagcgcg
     1681 ccgcgatcga gccgaaggac atcgactacg tggccgccca tgcaacggcg accaagatcg
     1741 gcgatcaggg cgagacggtc tccgtgaaga aggcgctcgg cgatcacgcc tacaaggtgg
     1801 cgctctcggc gaacaagtcg atggtcgggc acatgctcgg cgcggcgggc gcggtctccg
     1861 ccctcgcctg cgtcctcgcg attcgtgacg gcgtgatccc cccgacgatc aacctgatga
     1921 cgccggaccc ggattgtgac ctcgactaca cgccgaatgt ctcgcgcaag ctggacgtca
     1981 aggtcgcaat ggccaacggc tttggctttg gggggcagaa cgcgtcggcg gtcttcaagg
     2041 ctgtctagcg cgtgtcgcgc gaatccttgt ggcgaggacc cgtctcgccg aatgctttcg
     2101 ccacacgagc cgcccgtgaa cgcgattcgg ggtgtagctc gcaccgatcc cgatctggaa
     2161 tacagcgttc gtgcgtgggg cgggacttct atgtcccgcc tcgtggcgag aagaatcgct
     2221 gcgttgccag ctcaccgcgt gccagcgcga cggcgatctc gttatgtcgc gccacgatcg
     2281 acggcaggtc gacatcgacg atctgcccat cccggacgcg gacgcgcccc tcgatcacac
     2341 tgagatccac attccccggc tggcagaaga cgatcgccgc cagcggatcg tgcaccgctc
     2401 cccccgcaaa cggcagcgtg tcgacgcgaa tgccgatcag gtcaccccgc attcccggcg
     2461 cgagatagcc aacatccgtc cggccaagga cttccgcgcc accgcgcgtc gcgatccgaa
     2521 gcgcctcccg cgccgacatc gccaggggat cgccgccatc cgccacccgc tgcaacaaca
     2581 tcgcctgccg cgcctcggcg agcagatgcg atccgtcgtt ggaggccgag ccgtcgacac
     2641 cgagcccaac ccgcatcccg gcacgaagcc aggctccgac ccggggaatc cccgaaccga
     2701 gccgcatgtt cgaggtcggg caatgtgccg cgcccgtttg cgacgcaccg agccgcgtga
     2761 tctcgtcggc agctggatgc acgaggtgcg catgccagac atcctcgcca acccagccca
     2821 gatcctcgca gagctcgacc ggtgtccgcc cgaacgtctt ccggcagtag tcgtcctcgt
     2881 cccgcgtctc ggcgagatga gtgtgagaat ggacaccata ggaacgcgcg agcacgacgc
     2941 tctcccgcat caggtcaggg gaaacgctga agggcgaaca cggcgccagc acgatccgcg
     3001 tcatcgcacc atcactggcg tcgtggtagg tctcgatcgc acgccgcgag tcgcggagaa
     3061 tcgcgtcctc gtcctcgacg acggaatcag ggggcaatcc gcccttcgat tcaccgaccg
     3121 acatcgagcc gcgcgacgcg tggaagcgaa ccccgatctc ctccgcggct tcgatctggt
     3181 cgtcgatccg ggatccgttc ggccagagat aggtgtgatc gctcgcggtc gtgcaaccgg
     3241 aaagcaggag ctcggtgagg gcaaccttcg tgctgacctg gatcgcctcg ggtgtcatcc
     3301 ctccccagat cgggtagagc gtcttcagcc agtcgaagag caccgcgttc tgggcggcag
     3361 gaatcgcccg tgtcagcgtc tgatagaaat gatggtgggt gttgacgagg ccgggcagga
     3421 tcaccatgcc gcgggcatcg atcgcgtcat ccgcagtcgt ggggagttcg gaagccgggc
     3481 cgatctggcg gatcacgcca ccttcgatca ggaggcttgt gtccggcagc tcgcgcgagt
     3541 cgtcatccat cgtggcgacg agcgtcgcgt tgcgaatcag cagtgtcttg ccgcgaggct
     3601 cggtcgcacc gtcgctcgag atgttgtcca ttgcgtcctc cagacaggcg ggaaccgtgg
     3661 gcagagtcgg actttcccgg ccagacgatc ggggaggcgg aaccctcatg gaagacatta
     3721 tgcgggaatg cgccgggctc cgaaccggat cgaccggcga gactccacgg caccgtgact
     3781 cgcggcgcca tgcgcgctcg accgggccgc gatcatctcg ccagcaggtc gagggagacc
     3841 tgccacaggc gcgcggcctt cccgggatcg agcgcgtatg ccgcgacacc ccggcggacg
     3901 ggtgccacgt agggcacggc ctcattgcag tcctcgaaat accgccccgt caccccctcg
     3961 accaatggcg acgccgcgag aagaacggat gtcgcggcgc cttgctccgc gttcttccag
     4021 gaaacgtcgg tgctcgtcgg gtcgaaggtg gaggggctgt tcgccacgtc gccgatgtat
     4081 cgggacagac cggtgcctac gatgcgtcca gggttcaaca cattcaccgc gatcccatcc
     4141 tcatgccacc gccgggccgc ttccaccatg aagaggacgt tggccgtctt cgactggcta
     4201 taggcttgcc acgcgtcata cgggtggcgt tcgaactgga tgtcgtcgaa gaggacctcg
     4261 ccgttcacat gcccaacgga actgacgacg acgacgcggg cacgatgtgc cgccgcgaga
     4321 gacccgtgga gtccgagcgc cagtgcgaag tgtcccaggt gattcgtcgc gaactgcagc
     4381 tcccacccct ccgcggtgcg catctcgggt gacgccatga ttccggcatt gttgaccagg
     4441 atgtccagcg gatcctccca gaaggctacg aaggccgcga tcgacgcccg atcggcgaga
     4501 tcgagcggca tcacgagcat gcgtccctcc cccacggccg gcgcgatctc cgcggccgct
     4561 ttcatccccg cctccaggtt tcgcacgcca atcgtgacct cggctccggc cgatgccagc
     4621 gcgcgtgccg tctccaggcc aattcccgag gcgccgccgg tgacgatcgc gcgcttgccc
     4681 ctgaggtcga catccgcgac gacctccatc gccgtcgtcg tc
//
LOCUS       DASTMD010000003         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1428574, whole genome shotgun sequence.
ACCESSION   DASTMD010000003 DASTMD010000000
VERSION     DASTMD010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428574"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            447..1733
                     /gene="eno"
                     /locus_tag="VFQ54_00040"
     CDS             447..1733
                     /gene="eno"
                     /locus_tag="VFQ54_00040"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU0163395.1"
                     /translation="MVDSIIEAIHGREILDSRGNPTVEVEVYLTSGTVGRAAVPSGAS
                     TGAYEAVELRDGDKARYGGKGVLQAVNNVNEDLAEAVVGLDALDQIGVDNVMRETDGT
                     ANKGRLGANALLGVSLAVARAAAEEVGLPLYRYLGGPNARTLPVPMMNILNGGKHAEG
                     SSVDMQEFMVMPIGAETYTEGLRWGTEVFHTLKKILTSMGQSTHVGDEGGYAPSLASN
                     EQALQVVVEAIEKAGYTPGVDLAIALDPAATELFHDGNYVLPGEGTTFTGDEMAAFLT
                     GWTEKYPIVSIEDGLAEDDWETWKKLTATIGHQVQLVGDDLYVTNTERLQRGIDEKAG
                     NAILVKLNQIGTLTETFEAIELAHKAGFRAVISHRSGETSDTFIADLSVATNAGQIKT
                     GAPSRVDRVAKYNQLLRIEEELGDTAIYPGRKAFRA"
     gene            complement(1788..>2048)
                     /locus_tag="VFQ54_00045"
     CDS             complement(1788..>2048)
                     /locus_tag="VFQ54_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163396.1"
                     /translation="LYLTGLSDLSQADLNQLKEYSGFTDIEFNPKRSFNCQARSCALA
                     ATLLRLGLFDQALSRRESYMEIVWGDSGPIHESGPTRQPELF"
BASE COUNT          403 a          608 c          667 g          370 t
ORIGIN      
        1 cgtggtgctc atctcgggtt ctccagatac gtgttcattt ggacgcagaa aacccctcgg
       61 cagggacgag gggtttctcc acgcggtacc accctgcttc gctcggttcg gctgaaccgg
      121 cgcgcttgca tgctccgata agggggagcg aacccggatg atctactgac gcggtgcgcg
      181 ttcgatgacc ggctcgggag tgaattgacg attcctggcg tcggagccac tttcagcggt
      241 gtgtgactct ctctgtgcga ccgggaaatc gtcccgtctc cgtcacagcc tctcttcatg
      301 atgtaccgcg aagtatagca atagctgcca acaagcggcc tcggaatcgg cgagatcaat
      361 ggtcatcgtt tcgttatcta tcgcttgagt cgggcaccgt atacttgcgc caacttgccg
      421 gattctgagg agatgaagag gagcacatgg tcgacagcat catcgaggcg attcacggac
      481 gcgagattct cgattcgcgc ggcaacccga cggtcgaggt cgaggtctac ctcaccagcg
      541 gcaccgtcgg tcgggccgcg gtaccgtccg gagcttcgac gggagcgtat gaggcggtcg
      601 agcttcgcga tggcgacaag gcgcgctatg gcggcaaggg ggttctccag gccgtcaaca
      661 acgtgaacga ggatctcgcc gaagcggtcg tcggtctcga cgcgctcgac cagattggcg
      721 tcgacaacgt gatgcgggag acagatggca cggcgaacaa gggccgcctc ggtgcgaacg
      781 cgctcctcgg agtctcgctc gccgttgccc gcgccgccgc cgaggaggtc gggctgccgc
      841 tctatcgcta tctcggcggt ccgaatgcgc ggacgttgcc ggttccgatg atgaacatcc
      901 tcaacggcgg gaagcacgcc gagggctcca gcgtcgacat gcaggagttc atggtgatgc
      961 cgatcggcgc ggagacgtac accgagggac tgcgatgggg cacggaagtc ttccacaccc
     1021 tgaagaagat cctgacgagc atgggccaga gcacgcacgt cggtgacgag ggcggctacg
     1081 cgccgagtct cgcctcgaac gagcaggcgt tgcaggtcgt ggtcgaggcg atcgagaagg
     1141 ccggctacac gccgggcgtc gatctcgcga tcgcgctcga tcccgcggcg acggaactct
     1201 tccacgacgg caactacgtt ctcccgggcg aaggcacgac cttcacgggc gacgagatgg
     1261 cagcgttcct gactggctgg accgagaagt acccgatcgt ctcgatcgag gacggcctgg
     1321 cggaggacga ctgggagacc tggaagaagc tgacggcgac gatcggccat caggtccagc
     1381 tcgtcggcga cgacctctac gtcacgaaca ccgagcggct gcaacgcggg atcgacgaga
     1441 aggcgggcaa cgcgatcctc gtcaagctga accagatcgg cacgctgacc gagacattcg
     1501 aagcgatcga gctggcgcac aaggccggct tccgtgccgt gatctcgcat cgctccggcg
     1561 agacgtcgga cacgttcatc gccgatctct cggttgcgac gaacgcgggg cagatcaaga
     1621 cgggcgctcc ctcccgggtc gatcgcgtcg cgaagtacaa ccagctgctt cgaatcgagg
     1681 aagagctggg cgacacggcg atctaccccg gccgcaaggc gttccgcgcg taattgctga
     1741 cgtcgttgtg gcgcgaggat cgccacccac tcggtggcga tccattccta gaagagttca
     1801 ggctgtcgcg tcggccctga ttcatgtatg ggtcctgaat caccccagac gatctccata
     1861 taggactctc gtcgtgacaa tgcctgatcg aagagtccaa gtcgaagcaa cgttgccgcc
     1921 agcgcacacg agcgtgcttg gcagttgaac gagcgcttgg ggttaaactc gatatccgtg
     1981 aatcctgaat actctttgag ttggttcaga tcggcttggg aaagatcgct gagtccagtc
     2041 aggtatag
//
LOCUS       DASTMD010000004         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2500131, whole genome shotgun sequence.
ACCESSION   DASTMD010000004 DASTMD010000000
VERSION     DASTMD010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2500131"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..712
                     /locus_tag="VFQ54_00050"
     CDS             185..712
                     /locus_tag="VFQ54_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEU0163397.1"
                     /translation="MSDSPDGRRVLVAVVTGKVGETIQAWREQHDPEQALRLPPHATL
                     CYWAPEIEQIDLLGAQVRHAFDRPIAIELGGVHEFDNDDGTFYIEVNGTAELTEARHR
                     LFDGSHLQLQGRSDFTWHVTCVRYPDNAKREELRVAAESLSVAIAANPVWTIDTVAWL
                     QLRDGIYQPLATWNV"
     gene            1145..1933
                     /locus_tag="VFQ54_00055"
     CDS             1145..1933
                     /locus_tag="VFQ54_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEU0163398.1"
                     /translation="MDLDGTILEEGEQIRPEVIAALRRLSAQGVQCVTATGRQAAFQF
                     GLFARYELDPASTGVMSGLIGDEREIYIARDGAYHPHTAWNDAVRARWVDLFPTAWSL
                     LTEAKAEAESRGWQIELMQPAEIAAQRGLPTLVCADNIQGASLCLWIQEQIDARGLPL
                     ATNRNVRLVQIFDVLTGKGPALAELARELGIPNNQVLAVGDSSNDYTMLDGRYGFGFR
                     TATLNNAEDELKAIVRETGGYVASANAGLGTVEAIEALTFISLV"
     gene            complement(2268..3362)
                     /locus_tag="VFQ54_00060"
     CDS             complement(2268..3362)
                     /locus_tag="VFQ54_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEU0163399.1"
                     /translation="MPTELPFRISIDAEIASSAERAALALGNLNGSGRMLPNPTLLFQ
                     PFMRREALASSRIEGTQAEFGQLVLFEANEEADASNPDIQEVRNYLAALETGWNKPND
                     RPISPGFIMELHQQLLSGVRGASLNPGALRSVQVHIGSGGNDLSRARFVPPPPDFVRD
                     LVENLCNYIQNGNDLPFLVRLAVIHYQFETIHPFMDGNGRLGRLLMPIILGAWGRLDL
                     PLLYLSEYFEDHREEYIDLLLAVSQRGDWKEWLLFVLEAIESQANDAFRRGQRLLQLR
                     EELRSHYLHATRSSVALHVIDRLFENPAITVKNAAAHANVSTQSATKSIELLVEDGVL
                     EEVTGQKRNRVFLAPAIVEAMTARRSVRPE"
BASE COUNT          701 a         1069 c         1162 g          751 t
ORIGIN      
        1 ccgcgggatc tatccgagcg atcatcgcgg tgtgatgacg acgctggtga ttggatagat
       61 cgatcggcga cggcccggtt tttgggagac agggcgagca cgaggctcgc ccctatgatt
      121 accggtaatt ccgtaggggc gagcctcgtg ctcgcccggc ccaactgatg gagagaagca
      181 atccgtgagt gattcccccg atggacgtcg cgttctcgtc gccgtcgtga ctggcaaagt
      241 cggcgagacg atccaggcat ggcgtgagca gcacgatcca gaacaggcat tgcgcctgcc
      301 gccgcatgcg acgctttgct actgggcgcc ggagatcgag cagatcgacc tcctcggggc
      361 gcaggtccgg catgccttcg atcgaccgat cgcgatcgag ctcggtggtg tccacgagtt
      421 cgacaatgac gatgggacgt tctacatcga ggtgaacggc accgccgaac tcacggaggc
      481 ccggcatcgc ctcttcgacg gcagccatct ccagctccag gggcgatccg attttacctg
      541 gcacgtcacc tgtgttcgct atccggacaa tgcgaagcgg gaagagcttc gcgtggcggc
      601 ggagtcgctc tcggtggcga tcgccgcgaa tccggtctgg acgatcgaca ccgttgcctg
      661 gttgcaattg cgcgacggaa tctaccagcc cctcgcgacc tggaacgtct aggacgaaat
      721 ccggaatcgt cgcgatgggg agcacgatcc gtgggcgaac ggcaggcggg acaggccgtc
      781 cggacccatt cggcaagctc agggcaggct ctccggtccg gcagcggcgg caatgtcccg
      841 ctccttcgac aagctcagga caggctcccg aacgaccggg cgcaccgcgg actacgcatt
      901 cggtccagca gcatcttctg caaccgcttc ggatttcgct gtagaatgca tcacccctgg
      961 ttggatcgat ccagcacgct aggctatcgg gaccacaatg cccgacccgg cgcaggctgc
     1021 atccgtatcg cattgcatcg acctccaggg ggatcctttg acgaccgttt ccaccactga
     1081 ttcgactccg accgccgagg gtgcggaacc ccacgcttcg ctaccgccga tcgcgatgat
     1141 tgccatggac cttgacggta cgattctcga ggaaggtgag cagatccgtc ccgaggtgat
     1201 cgctgctctc cgtcgtctct ctgcgcaggg ggttcaatgc gtaacggcga cgggacgtca
     1261 ggccgcgttc cagttcggac tcttcgcccg atacgagctg gatccggcgt cgaccggagt
     1321 catgtcgggg ttgatcgggg acgagcgcga gatctacatc gcgcgggacg gcgcgtatca
     1381 cccccacacc gcctggaacg atgcggtgcg ggcgcgttgg gtcgacctct tcccgacggc
     1441 ttggtcgctg ttgaccgagg cgaaagcgga ggcggaatcg cgcggctggc aaatcgaatt
     1501 gatgcagccg gccgagatcg cggcgcagcg cggtttgccg acgctggtct gcgcggacaa
     1561 tatccagggc gcctcgctct gcctctggat ccaggaacag atcgatgcgc gcgggctgcc
     1621 actggcgacg aatcgcaacg tccggctggt ccagatcttc gatgtgttga ccgggaaagg
     1681 accggcgctc gccgaactgg cgcgtgagct ggggattccg aataaccagg tgctggcggt
     1741 cggtgattcg agcaatgact acaccatgct cgatggccgc tatggcttcg gattccgcac
     1801 ggcgacgctc aacaacgccg aggatgaact gaaggcgatc gtccgggaga ccggcggtta
     1861 cgtcgcctcg gcgaacgccg ggttggggac ggtggaagcg atcgaggcgt tgacgttcat
     1921 ctctctcgtc tgacccggac gttggcgctt ggcttcctgc ctgaattacc tgccgatgaa
     1981 ccgtagacgc ggcccctgtg gccgcttcgg gtcggcgcag ccgatccgct tccgggagcg
     2041 atccgcaggg tgcgttcgtg tcggagtccg gcggagttta ccctgagcct gttgaatggg
     2101 ccggatgtgc ttcgcacggg gatttctcca ttccgctgcg cttcagtcga aatgacatcc
     2161 atttgtgcgc cagcattgga gataaagtcc agccctacaa acctggctga acgaaagcct
     2221 gtccatcgat atagattggg ctgtggtttc gacaagtcta cggtgtctca ttcggggcgc
     2281 acagaccggc gagccgtcat cgcttccacg attgccggag ccaggaatac ccggttccgc
     2341 ttctgccccg tcacctcttc gaggacgcca tcttcaacaa gtaactcgat ggatttcgtg
     2401 gctgactgcg tcgatacatt ggcatgagca gctgcgtttt tcaccgtaat cgccggattc
     2461 tcaaatagtc tgtcaatgac atgcaacgcg acagacgagc gcgttgcatg taagtagtgc
     2521 gaccgaagct cttcccgcaa ttgcaatagt cgctgccctc gccggaatgc gtcattagcc
     2581 tgcgattcaa tggcttcgag gacaaagagg agccattctt tccaatcacc cctttggctg
     2641 acggcgagca ggagatcgat atattcttcc cggtgatcct cgaaatattc actcaggtag
     2701 agcagcggca ggtcgagccg tccccacgcc ccgagaatta tcggcatcag gagtcgccca
     2761 agtcgtccgt taccgtccat gaatgggtgg atggtttcga attggtagtg gatgactgcc
     2821 agcctgacga gaaaggggag gtcgttgcca ttctggatgt agttgcagag gttttcgacg
     2881 aggtcgcgga cgaagtcagg cgggggggga acgaatcggg cacgagacag atcgttccct
     2941 cccgatccaa tgtggacctg gactgatctc agcgcgccgg gattcagaga ggctcctcgc
     3001 acgccgctga ggagctgctg gtgcagctcc atgatgaatc caggcgagat cgggcgatcg
     3061 ttcggtttgt tccagccagt ctcgagagcg gcgagataat tccttacttc ctggatgtcg
     3121 ggattggacg cgtctgcctc ttcgttcgct tcgaagagga cgagttgtcc gaactcggct
     3181 tgcgttcctt cgatacgact cgatgccaac gcctcgcgtc tcatgaaggg ttgaaagagg
     3241 agtgtagggt tgggaagcat gcggccggag ccgttgagat tgccaagtgc cagtgccgcc
     3301 cgttcggcac tacttgctat ctcggcatcg atactgatcc ggaatggtag ctcggtcggc
     3361 acgaacgccc atccaggatt cccggtccgt gagatgatgt tgatgagggt cccgggggcg
     3421 tcagcaagga actgatctgg attcatgctc atcccatatg tgtgtcaggt tacatataga
     3481 ttcttatatg taactttgtt ggcgaaaagt tacctaacac atctatggta tcgcaaagca
     3541 agcatgatag cgtgggtgct tcgaagctat tgtcgccaga cggtgccgcg ccgctcatac
     3601 tcgaggttgc gctcgacgcg ggcggcgatc tccgctccgg cgtagcgctc gacgagccag
     3661 agcgcgagat cgagcccgga tgt
//
LOCUS       DASTMD010000005         1783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2143024, whole genome shotgun sequence.
ACCESSION   DASTMD010000005 DASTMD010000000
VERSION     DASTMD010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143024"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFQ54_00065"
     CDS             <1..275
                     /locus_tag="VFQ54_00065"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163400.1"
                     /translation="HHGFVTDLVPDYLQWADQIFSCGPTPMFLSLRDVVLRHRVGRTP
                     GVQVSTERTMACALGACLGCVVETRSGMQTSCVDGPVFDMEQVVWS"
     gene            272..1270
                     /locus_tag="VFQ54_00070"
     CDS             272..1270
                     /locus_tag="VFQ54_00070"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU0163401.1"
                     /translation="MSERIAPDLSVDLAPRNPRELVLRNPVIPASGCFGYGLEYSSFV
                     DVERLGAFVSKGITPHARTGNQMPRLAETPAGMLNAIGLQNPGIRNFIRKYPPIWEQW
                     DVPAIVNVSAESVEDFAAMARQLDDVPGIAALEINISCPNVNAGGYCFGWDPGMSADV
                     TRAVRAATTLPIIIKLSPGAVDIISIAQAVEEGGADAVSLINTLVGMQIDVKRRRPVL
                     ANVTGGLSGPAVKPVAVRMVYQVASAVGIPVVGLGGISNLDDALEFFMAGASAIEVGT
                     AIFTDPTMLVRLIDDLEAWMIRNSVSRLSEIIGVANPHYRSHVETSEEMFAEMIDG"
     gene            1267..>1783
                     /locus_tag="VFQ54_00075"
     CDS             1267..>1783
                     /locus_tag="VFQ54_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU0163402.1"
                     /translation="MRYLYILLVLVPITGILDIVGIDNHPLMFVLAAFSLVPLAALLG
                     NATEEVALFTGQRIGGLLNATLGNAAELIITIVALREGLLNVVKASIAGSIIGNMLVV
                     LGAAVLLGGLKNGEQSFDERVAGTNASMMSLAIVVLMIPAVFALGSSSDRLSSDDVVN
                     LSDGAAIILIIL"
BASE COUNT          289 a          546 c          593 g          355 t
ORIGIN      
        1 gtcatcacgg cttcgtgacc gatctcgtgc ctgactacct gcagtgggcc gaccagatct
       61 tctcttgtgg accgacaccg atgttcctct cgctgcgcga tgtcgtcctg cgccatcgcg
      121 tcgggaggac ccctggcgtc caggtctcga ccgagcggac gatggcgtgc gcgctcggcg
      181 cctgcctcgg ctgcgtcgtc gagaccagat ccgggatgca gacctcgtgt gtggacggac
      241 cggtcttcga catggaacag gtggtgtggt catgagcgaa cggatcgcac ccgatctcag
      301 tgtcgacctg gcgccgcgga acccgcgcga gctcgtgctg cgcaacccgg tgattcccgc
      361 gtcggggtgc ttcggttacg gcctcgagta cagcagcttc gtcgacgtcg agcggctggg
      421 cgccttcgtc agcaagggga tcacgccgca cgcccgcacc gggaatcaga tgcctcgcct
      481 ggcggagact ccggccggaa tgctgaacgc gatcggactc cagaatccgg ggattcgcaa
      541 cttcatcagg aagtaccctc cgatctggga gcagtgggat gtgccggcga tcgtgaatgt
      601 ctcggccgag agcgtcgagg atttcgcggc gatggcgcgg cagctggacg atgtgccggg
      661 tatcgcggcg ctggagatca atatctcctg tccgaacgtg aatgccggtg gctactgctt
      721 cggctgggat cccgggatgt cggcggacgt gacgcgggcc gttcgcgcgg caacgacgtt
      781 gccgatcatc atcaaactga gccccggcgc ggtcgatatc atcagcatcg cgcaggcggt
      841 cgaagagggt ggggcggacg ccgtctccct gatcaacacg ctggtcggga tgcagatcga
      901 cgtcaaacgc cgtcgcccgg tgctcgcgaa tgtgacgggt ggtctctccg gcccggccgt
      961 gaagccggtc gcggtgcgca tggtctacca ggttgcgtcc gcggtcggaa tcccggttgt
     1021 cggtctcggc gggatctcaa atctcgatga tgcgctcgaa ttcttcatgg cgggcgcttc
     1081 ggcgatcgag gttggcacgg cgatcttcac ggatccgacg atgctggttc gcctgatcga
     1141 cgatcttgag gcatggatga tccggaacag tgtctcgcgt ctgagcgaga tcatcggcgt
     1201 cgcgaatccc cactaccggt ctcatgtcga gacctccgaa gagatgttcg cggagatgat
     1261 cgacggatga gataccttta cattctcctc gtcctggtgc cgatcacggg catcctcgat
     1321 atcgtcggta tcgacaatca cccgctgatg ttcgtgctcg cggccttttc cctggtgccg
     1381 ctcgccgcgc tgctcggcaa cgcgacggag gaagtcgcgc tcttcaccgg ccaacggatc
     1441 ggtgggttgc tgaacgcgac gctcgggaat gcggcggaac tgatcatcac gatcgtggcg
     1501 ctgcgcgagg gactgctcaa cgtcgtcaag gcgagtatcg ccggctcgat catcgggaac
     1561 atgctcgtcg tgctcggagc ggcggttctc cttggtggtc tcaagaacgg cgagcagtca
     1621 ttcgacgagc gcgtcgccgg gaccaacgcc tcgatgatgt ccctcgcgat cgtcgtcctg
     1681 atgatcccgg ccgtcttcgc gctcggatcg tcgagcgatc ggctctcgag cgacgacgtc
     1741 gtgaacctga gcgacggggc ggcgatcatc ctgatcattc tct
//
LOCUS       DASTMD010000006         3504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_893064, whole genome shotgun sequence.
ACCESSION   DASTMD010000006 DASTMD010000000
VERSION     DASTMD010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893064"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VFQ54_00080"
     CDS             complement(<1..539)
                     /locus_tag="VFQ54_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163403.1"
                     /translation="MHRRDRRVGLVFDDRYLTHNPGPYLIAYKDPYPFADPVPHVSSP
                     AVAIRSKQLLDLFNITDAMQRIPAFEADDEMLLAYHTREYISRVQDIGKTGGDTGRGA
                     PIGRAGDRIARLSAGGVIAATSAVMQGDVHHAYALVRPPGHHAMSDRGMGFCVFNNIA
                     LAARHAQRAHGAERVVILD"
     gene            complement(717..1793)
                     /locus_tag="VFQ54_00085"
     CDS             complement(717..1793)
                     /locus_tag="VFQ54_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEU0163404.1"
                     /translation="MRRILGRAVAGGTLALAMCLPLATSTPSVFAQSTSVEAWATIGA
                     THPAPGCSVDVALQLQDGGSTISGADVAIVFSLDSTGEVISSDEGQTDGDGVANLTYD
                     TSNASAGDKGWLEVHVNGQYLGGQTIWADGDSCDQGSTLVDMSGSVPTTATSTSDSSS
                     SSSDDSSDSSASVDDSSDTVMIPNVSAYLQQRPLSCEFAALSIATGALGDWVSEYDFD
                     DLVGWSDNPHWGFRGDINGEWGGTTDYGVYAEALVPALNQYGFQGDVFYGGVDTLKAE
                     IAAGNPTLVWMGLLGDQRDYETTSDGTTYQLTAGEHAMVAYGYDDGGVYVSNPGSGTL
                     QYYDWGTFEYMWGILDDMALSVHW"
     gene            complement(2129..3163)
                     /locus_tag="VFQ54_00090"
     CDS             complement(2129..3163)
                     /locus_tag="VFQ54_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEU0163405.1"
                     /translation="MRRILGRALVGMGIAASLCLPMTTPAAFAQSASIDAWATIDATH
                     PAPGCTVGVTVELRDGGSPLTAADVSILLSDDGTGEVVSSDRSVTGDSGVAYLSYDAP
                     SSAKNWLEIDVNGQYLGGVTIWADGSSCDQGSSVVDFSGDVPAIATSGGDDVSSAEAS
                     SDGGAVILPNVSAYQQQRPLSCEYASLAIATGALGNWISEYDFDDLVGWSDNPHWGYR
                     GDIWGVWGNTTDYGVYAEALVPALNAYGFNANVFYGGTDNLTAAIDAGQPTVVWLGLW
                     GDQSVYPATSDGTSYQVTAGMHVMVVYGYDDGGVYVSDPGLGTLHYYDWSTFLYYWDV
                     MDQMALAVSN"
BASE COUNT          642 a         1067 c         1134 g          661 t
ORIGIN      
        1 cagtcgagga tgacaacgcg ttcggcgccg tgcgcgcgct gggcgtgacg tgccgcgagc
       61 gcgatgttat tgaagacgca gaaccccatt ccgcggtcgg acatcgcgtg gtggccgggg
      121 ggccggacca gggcgtaggc gtgatggaca tcgccctgca tcacggcgct ggtggcggcg
      181 atcaccccgc cggcggagag gcgggcgatg cgatccccgg cgcggccgat cggcgcgcca
      241 cgcccggtgt cgccgccggt cttgccgatg tcctgaacgc gcgagatgta ttcccgtgtg
      301 tgataggcga ggagcatctc gtcatcagct tcgaaagccg ggatccgctg catggcatcg
      361 gtgatgttga agagatcgag gagttgtttc gagcggatcg cgaccgccgg gctggagacg
      421 tgcggaacgg gatcggcgaa gggatagggg tccttgtagg caatcaggta tggaccaggg
      481 ttatgcgtta ggtatcgatc gtcgaagaca agtccgacgc gacggtcgcg gcgatgcatc
      541 ctgatgtagc ctccctggct tcctcatgac tggttccggc ggggtgtgcc gttcgctgac
      601 gcgagtttcc atcctaaccg ctcaggtgtc gaccgtggta ccgcatgcat acaaaagcga
      661 ggggtcccag gcgacccctc gcttttgtat gcaatgacca tgatgcggcc gggaatctac
      721 cagtgcaccg acagtgccat atcgtcgagg atgccccaca tgtattcgaa ggttccccag
      781 tcgtagtact ggagtgttcc cgaacccggg ttcgaaacgt agacaccgcc atcgtcgtag
      841 ccgtaagcga ccatcgcgtg ctcgcccgcg gtcagctggt aggtggtgcc atcgctcgtg
      901 gtctcgtagt cgcgctggtc gccgaggagt cccatccaga cgagcgtggg attcccggcg
      961 gcgatctccg ccttcagggt gtcgacgcct ccgtagaaga cgtccccctg gaaaccgtac
     1021 tggttcaacg cagggaccaa cgcttcggcg tagacgccgt aatcggtcgt tccgccccac
     1081 tcgccgttga tgtcaccgcg gaacccccag tggggattgt ccgaccagcc gacgagatcg
     1141 tcgaagtcat actcggacac ccagtcgccg agggcgccgg tcgcgatcga gagtgcggcg
     1201 aattcgcaac tcagcgggcg ctgctgcagg taggcggaga cgttcgggat catcacggtg
     1261 tcgctggaat cgtccaccga agccgacgag tccgaagaat cgtcgctcga tgacgaggag
     1321 ctgtcggatg tgctcgtcgc cgtcgtcggc acgctcccgc tcatgtcgac aagcgtgctg
     1381 ccctggtcgc agctgtcgcc gtcggcccag atcgtctggc caccaagata ctggccgttg
     1441 acatggacct cgagccaacc cttgtcgccg gcggaggcat tgctggtgtc ataggtgagg
     1501 ttcgccacgc catcgccatc ggtctggccc tcgtcgctgg agatgacctc accggtcgag
     1561 tcgagcgaga agacgatcgc gacatcggcg ccggagatcg tggatccgcc gtcctgaagc
     1621 tggagggcga catcgacgga acaaccggga gccggatggg tggcgccgat cgtcgcccag
     1681 gcttcaacgg atgtggattg tgcaaagacc gatggtgtcg acgtcgcgag cggcaggcac
     1741 atcgcgagag cgagcgttcc tccggcaacg gcgcggccca gaatacggcg catagatctc
     1801 cccttgctcc ccacggcgcg aaccacgccg gttggtcgac gtgatgcgtc gtcaaaatcc
     1861 ctgttgaccg ggggccaatt cccaccccgg gtcggacttg ccaagtctcc acgactctac
     1921 caacaaatgg aaattaccgc tacttgtgaa cgttcatgtt gcccgggtgg caatccacgt
     1981 cgacgtcctg gcgcgattct gatcgattta cggttctttt cggtctcctg gatttttcgt
     2041 tgtgccagaa cggaacgaag aggaacgcca cccaggcatt gggtggcgtt cctcttcatc
     2101 ggcgggactg cggaattgtc ggaggcgatc agttcgagac ggcgagcgcc atttgatcca
     2161 tcacgtccca gtagtagagg aacgtactcc aatcgtagta gtggagcgtt cccaatcccg
     2221 ggtcggaaac atagactccg ccatcgtcat agccatagac gaccatcaca tgcatgccgg
     2281 cagtcacctg gtagctcgtg ccgtcactcg tcgccgggta gacgctctgg tctccccaga
     2341 gaccgagcca gacgacggtc ggctgaccgg catcgatcgc ggcggtgagg ttgtccgtcc
     2401 cgccgtagaa gacattggcg ttgaatccgt aggcgttcag ggccgggacg agagcctcgg
     2461 catagacgcc gtaatccgtg gtgttgcccc agacgcccca gatatcgccg cggtagcccc
     2521 aatgcgggtt gtccgaccag ccgacgagat cgtcgaagtc gtactcggag atccagttcc
     2581 cgagggcgcc ggtcgcgatc gcgagcgacg catactcgca actgagtggc cgttgctgct
     2641 gatacgcgga gacgttcggc aggatcaccg cgccgccatc actcgacgcc tcggcgctgg
     2701 agacgtcgtc cccaccggat gtcgcgatcg ccgggacatc gccactgaag tcgacgaccg
     2761 aactgccctg atcgcagctg ctgccgtcgg cccagatcgt gacgccgccg aggtactgcc
     2821 cgttgacgtc gatctcgagc cagttcttgg ccgatgaggg cgcatcgtag gagagatagg
     2881 cgacgccgga atcccccgtg accgaacggt cgctggagac gacctcaccc gtcccgtcat
     2941 cggacaggag gatcgatacg tctgctgcgg tgagtggcga gcctccgtcg cggagttcga
     3001 cggtcacgcc gaccgtgcat ccgggagcgg ggtgggtcgc atcgatcgtc gcccaggcat
     3061 cgatcgatgc ggactgcgcg aaggcggctg gtgtcgtcat cggcaggcag agcgacgcgg
     3121 cgatgcccat tccgacgagt gcgcggccga gaatccggcg catggttgtc tccccctcag
     3181 atttcgacgt gctgtcggcc acactttccc gatagccgtt cgacggcatt gccggagcgc
     3241 cccgtggcaa cgggacgttc cacatcctgg catcgacttt accaacattc cgcctgaagt
     3301 gctacttcag aacatctctc gttggatgtt acgggtgtgt atttatctta gaagtcgagc
     3361 cgccaaaggg tgcgttcctt cgcggcaatg acatcggaaa atgtcccaat cgcggcgtat
     3421 ccacaagatt tgggcacaag atcacgactt tggcgattgg tcttcgctgg taggaaggtt
     3481 ctcgctgagg aaatggcgac gggc
//
LOCUS       DASTMD010000007         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1339795, whole genome shotgun sequence.
ACCESSION   DASTMD010000007 DASTMD010000000
VERSION     DASTMD010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339795"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFQ54_00095"
     CDS             <1..753
                     /locus_tag="VFQ54_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0163406.1"
                     /translation="IGPNGAGKTTLLSVVGADRFPSKGEVTILGEEFGKTDMRTLRGK
                     IGRVDPNLRMLDWVNAEEAVLTGLKNQVWPKWDEWTPDDHAHARDLLDLVGCLDFAEQ
                     EIKTLSQGERQRIRIARALIAQPKLMLLDEPATGLDFPGREALLASIDELTTSHPDLP
                     TIMVTHHLEELPTTITHVMLMKEARVLAIGEASEMLTSAWISECFGFPITVQQFDGRW
                     SARAEAGWTASRHRRVPPVVPEGAVDPYPDEV"
     gene            905..1993
                     /locus_tag="VFQ54_00100"
     CDS             905..1993
                     /locus_tag="VFQ54_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEU0163407.1"
                     /translation="MVETANGEPRGPQYDTIITGGELVDPGAGNVGRFDIAIKDGKVA
                     ALGGPFPPSSARRQIDARGQVVTPGLVDIHTHIYWGATYWGIEPDPIAARTGVTTWVD
                     AGSSGSYSFPGFREYVVNRSASRVFAFLNLSTIGLIAPTWEFANPDYWDVELAVETVE
                     RNRDVVVGIKARIDAKTTRGVGIEPLARARELADRVGMPLMTHIGTAPPSMDEVIPYL
                     RPGDILTHCFTWQDMSIADESGKIRPEIRQLQDEGLILDVGHGAGSFGFGIAESMYAQ
                     GVLPDVISTDIHQLAVQGPMFDLPTTMSKFLTLGMNLPQVIACTTVNAARAVKLEGAG
                     SLAVGSVADIALFRIEEGEQSYYDTKAE"
BASE COUNT          362 a          647 c          673 g          353 t
ORIGIN      
        1 attggcccga acggcgcagg caagacgacg cttctctcgg tggtcggcgc cgatcgcttc
       61 cccagcaagg gtgaggtgac gatcctcggc gaggaattcg gaaagacgga tatgcgtacg
      121 ttgcggggaa agatcggccg cgtcgatccg aatctccgca tgctcgactg ggtgaatgcc
      181 gaggaagccg tgctgaccgg actcaagaac caggtctggc cgaagtggga cgaatggacg
      241 ccggacgatc acgcccacgc tcgcgacctg ctcgacctcg tcgggtgcct cgatttcgcg
      301 gagcaggaga tcaagacgct ctcccagggc gagcgccagc ggatccggat tgcccgggca
      361 ctgatcgccc agccgaagct gatgctgctc gacgagccgg cgaccggcct cgattttccc
      421 ggccgggagg cgctgctggc gtcgatcgac gaactgacga cctcgcatcc cgacctcccg
      481 acgatcatgg tcacccatca tctcgaggaa ctgccgacga cgatcacgca cgtgatgttg
      541 atgaaggaag cccgggtgct ggccatcggc gaggcgagcg agatgctgac gagcgcctgg
      601 atctcggaat gcttcggctt tccgatcacc gtccagcaat tcgacgggcg ctggtcggcc
      661 cgcgcggaag cgggctggac ggcgagccgg caccggaggg tgccgccggt ggtgccagag
      721 ggtgcggtcg acccataccc cgatgaggta taggcgaatg ttcgctctgc ggccgatcct
      781 gtcccggctg tgttctgtat tctcgccact cgcgtgaacc ccgcctgtgc gctaagatgc
      841 cgcccagtat cggcgcgacg gataaaccgc cgtgcacctc cgcgcattca ttgggagttg
      901 gtctgtggtt gaaacggcga acggtgagcc ccggggaccc cagtacgaca cgatcatcac
      961 cggcggcgag ctcgtcgacc ccggcgccgg caatgtcgga cggttcgaca tcgcgatcaa
     1021 ggatggcaag gtcgccgcgc tcggcgggcc gttcccgccg tcgagcgccc gccggcaaat
     1081 cgacgcccgc ggccaggtcg tcactcccgg tctcgtcgac attcataccc atatctattg
     1141 gggcgctacc tactggggga tcgagccgga tccgatcgcc gcgcggaccg gtgtcacgac
     1201 ctgggtcgac gccgggagct ccgggagcta cagcttcccc ggtttccgtg aatacgtcgt
     1261 caatcgcagt gcgtcgcggg tcttcgcctt tctcaatctc tcgacgatcg gactgatcgc
     1321 cccgacgtgg gagtttgcga atcccgacta ctgggatgtc gaactcgccg tcgagacggt
     1381 cgagcgcaat cgggatgtcg tcgtcgggat caaggcgcga atcgacgcga agacgacgcg
     1441 cggcgtgggg atcgagccgc tcgcccgggc gcgggagctc gccgatcggg tcgggatgcc
     1501 gctgatgacg cacatcggca ccgcgccgcc gtcgatggac gaggtcatcc cgtatctccg
     1561 tcccggtgac atcctcaccc actgtttcac ctggcaggac atgagtatcg ccgacgaatc
     1621 cggcaagatt cgtcccgaga tcaggcaact gcaggacgag ggattgatcc tcgatgtcgg
     1681 ccacggcgcg ggatcgttcg gcttcggcat cgccgagtcg atgtatgccc agggcgtcct
     1741 gccggacgtg atctccacgg atatccatca gctggcggtg caggggccga tgttcgacct
     1801 gccaacgacg atgtcgaagt tcctgacgct gggaatgaac ctgccccagg tcatcgcctg
     1861 caccacggtc aatgccgcgc gagccgtcaa gctcgagggg gcgggctcgc tcgccgttgg
     1921 cagcgtcgct gacatcgcgc tcttccggat cgaggagggc gagcagagct attacgacac
     1981 gaaagcggaa tgaacattgc atcatttctc gctgccttgg acgtgatgac gacga
//
LOCUS       DASTMD010000008         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_89922, whole genome shotgun sequence.
ACCESSION   DASTMD010000008 DASTMD010000000
VERSION     DASTMD010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89922"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VFQ54_00105"
     CDS             <1..1121
                     /locus_tag="VFQ54_00105"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0163408.1"
                     /translation="VAVGTIPLVVVGIFGGAIVDRIGYRTASVIADVLSGLSVALIPL
                     LHATTGLSFWQLLVLVFLGSFFDGPGSTARQALYPELAKGTGFTLDRANTGYNLTSRI
                     ASLLGTPLAGVLIAVLGPSDLLWVDAVSFACSAAIVLALVPKIPVEHAMAAVTGLRSY
                     LDDVRSGFRFLLETRFLLTLILFSCLGQLLAEPIYGVILPVYANQVLDSAAELGFIFA
                     ALGAGSIVGNLIYAWVGPRFTRSQFLLGGFAVRALGFCVMLTTPDWWVIAAAIFIGAV
                     ALEPTNPMFMSLFQEQVPSGMRGRVYGALTAISAGARPTGLVGYGFLMSHMGLHPTLV
                     LFVALNLALAVGMILSPFLRHIPQPTDVTSGRTVAEIA"
     gene            2043..>2319
                     /locus_tag="VFQ54_00110"
     CDS             2043..>2319
                     /locus_tag="VFQ54_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163409.1"
                     /translation="MKFVSIAAVFVLLVALSGAAWFNLGSGGGGNGDQSSVLAAPSDT
                     GSASSSCDVRYMPSQGYATTPTSATCDADEWKVPTAAYTVPAKSDCKV"
BASE COUNT          360 a          801 c          701 g          457 t
ORIGIN      
        1 ccgttgcggt ggggacgatc ccgctcgtcg tggtcgggat ctttggcggc gcgatcgtcg
       61 atcgcatcgg ctaccgcacg gcgagcgtca tcgcggacgt cctgagcggg ctttccgtcg
      121 cccttatccc gcttctccac gcgacgaccg gtctcagctt ctggcagctg ctcgtgctcg
      181 tctttcttgg ttcgttcttc gacggcccag gcagtaccgc ccggcaagcg ctctatccgg
      241 aactggcgaa gggcacgggg ttcaccctcg accgggcgaa taccggctac aacctgacgt
      301 cacgcatcgc aagccttctc ggcaccccac tcgccggtgt cctgatcgcg gtactcggtc
      361 cgagcgatct cctctgggtg gacgcggtga gcttcgcctg ttccgccgcg atcgtcctgg
      421 ccctggttcc gaagattccg gtcgagcacg cgatggcagc ggtgaccggc ttgcgcagct
      481 atctcgacga tgttcgaagc ggcttccgct tcctgctcga gacgcgattc ctcctcacgc
      541 tgatcctctt ctcgtgtctg ggccagcttc tcgccgagcc gatctatggc gtgatcctgc
      601 ccgtctatgc caaccaggtc ctcgacagtg ccgccgagct cggcttcatc ttcgccgccc
      661 tcggtgccgg ctcgatcgtc ggcaacctga tctatgcctg ggtcggcccc cgattcacgc
      721 gaagtcaatt cctcctcggc ggcttcgccg tgcgcgcact gggcttctgc gtgatgctga
      781 caacgcccga ctggtgggtg atcgccgcgg ccatcttcat cggcgccgtc gcgctcgaac
      841 cgaccaatcc gatgttcatg tcgctcttcc aggagcaggt gccgtccggc atgcgcggac
      901 gcgtctatgg cgcactcacg gcgatttccg ccggagcccg accgaccgga ctcgtcggct
      961 acggcttcct gatgtcgcac atggggctgc acccgacgct cgtgctcttc gtcgccctca
     1021 acctcgcgct tgcggtcgga atgatcctgt cccccttcct ccgtcacatc ccgcaaccca
     1081 cggatgtgac cagcggcagg acggtggccg agatcgcctg atcctgtgca acccctgacc
     1141 gcaactcgcc tcatcctcat gcgtttacga tgatgaccgg tacggagaag ccggtctcga
     1201 tcgcaacgcg gtgagggatg aggatgcgcg atggcccaga tcgccaggtg gacgcggtcg
     1261 actgctggtc acctggcaaa atgcagacgg ggcgtggcca atcgccggga acgtctacgg
     1321 ggtccagatg gggcaattcc tcgccagttt ccccaacgga ttcatgttca cccgggaatc
     1381 agccaatagt ccggggctgc tcgcgggtat ccagaacttc cggggctgga gagaggcggc
     1441 acattccgcc gatcagctcc tggccgctcc agcgagactt ttgtccactg ggctgacggc
     1501 tgcagtggcc gccctacgca cgaacgacgt acggagcgga gcgtgcggcg cgaccaacct
     1561 cggcgcggtc gttggcacag cgttcgtgcg tagggacgga cttcaatgtc cgtccgtcga
     1621 tcgcagatcg acatgatcgt cgcaacacaa tgcctcgcgc tcgccgatat tgcattcccg
     1681 tccccacggc ccttgttgcc gtccgttacc aatatcggcg aagccggtcc ggcccgcgtc
     1741 agcctgcggt cggatcggtt tcgccacgag atttctccac tccggcggga ggcttcgctt
     1801 cggtcgagct ggtgggtaga gagacggcta cacatcccgt tctcccggcg acgatccggc
     1861 ggtatagtgg tgctatcctc cgcggctcaa tctcatgccg ggccgcgacg gcgtcagccc
     1921 gcgatccagt cagggagaag atcctcatgg cgcagatggg aatggccccg acctccgctc
     1981 cgcctccaac tccgtccgca tcggattcac gccgcggaac gccgaccggt cgagtccggt
     2041 ccgtgaagtt cgtgtcgatc gcggcggtct tcgtcctcct cgtcgcattg agcggagccg
     2101 cctggttcaa cctcggatct ggtggaggcg gcaacggcga tcaatcctcc gtcctcgctg
     2161 cgccaagcga taccggctcg gccagctcct cctgcgacgt caggtacatg ccatcgcagg
     2221 gatacgcaac gaccccaacc tccgcaacat gtgacgccga cgagtggaaa gttccaacag
     2281 cggcctacac cgtgccggca aagtcggatt gcaaggtcc
//
LOCUS       DASTMD010000009         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2679119, whole genome shotgun sequence.
ACCESSION   DASTMD010000009 DASTMD010000000
VERSION     DASTMD010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2679119"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VFQ54_00115"
     CDS             <1..1058
                     /locus_tag="VFQ54_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU0163410.1"
                     /translation="AKGWDVRVLDSLERQTHRTGKPAWVPSDVEFIHGDVRNRSDWER
                     ALDGIDVVFHEAAYGGYMPEIAKFMHVNSYGTALMLETIRNKDLPVRKIVVASSQAVY
                     NEGAYSCPQHGTFYGETRPIAQLQAGDYAIHCPVCGAPSTPVPTDEAAPMGGENVYAI
                     SKSDQERLVISFGRATGIPVVALRYSCTYGPRQSLFNPYTGVIAIFCTRLINDLAPVV
                     YEDGGQSRDLVFVEDIARANLLVATDDRANGRIMNVGTGNAAAIGDLARLLASKLGKD
                     IAPQIPGEFRPGEMRALISDISRIQELGFAPVVDLPTGIDRYLAWIGEQGDIGEYFSA
                     AERRLRARQVVKKARAS"
     gene            1055..1831
                     /locus_tag="VFQ54_00120"
     CDS             1055..1831
                     /locus_tag="VFQ54_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0163411.1"
                     /translation="MTSEDRPRPTAAIVIPCLNEEESIGGLIAEILAVAADPMLPIVV
                     NGIYVVDNGSTDRTADVARSVGAKVVSEPRRGYGRACLTGVLATGDVDLIVLMDGDRS
                     DVPAELPLLLAPILRGEAELVLGSRMLGSYEPGSLLPQQIFGNRVAAILFRIVYRVRI
                     TDIGPFRVIPRDALLALGMREMTYGWSIEMIARAVRAGLTVREVPVTYRKRAGGVSKV
                     SGNFRASIRAGYRILGAILRARSDNAPLPDDLARTIGRSQ"
     gene            1828..2547
                     /locus_tag="VFQ54_00125"
     CDS             1828..2547
                     /locus_tag="VFQ54_00125"
                     /inference="COORDINATES: protein motif:HMM:NF021365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEU0163412.1"
                     /translation="MSRSNRLLMIAARAPIPGETKTRLGRAIGMEQAARLYRAFLVDL
                     AARFTGDGEYDVAWTHSPPEADFAAIHADLCGGVRPGALFVPQEGPDWGTRQANLLRW
                     GVDHGYAHTILIASDSPHLGGETIDAAFAVLERADVAIGRVQDGGYYLIGLNRFADIL
                     SGVAMSTSSAADGVVLRAGQLGLRVAEVPATFDIDEASDLSLLVAALAPGGDAAPATW
                     RTLAALGLRDGERVVIPETTS"
     gene            2544..3242
                     /locus_tag="VFQ54_00130"
     CDS             2544..3242
                     /locus_tag="VFQ54_00130"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU0163413.1"
                     /translation="MTFLNIAHRGASGYAPENTRAAFDLAIEMGADMIETDVQLTRDG
                     QPVLIHDGVVDRVSNGFGPIGDYTLAELEALDFGAWYDSRFAGQRIVTLAVFIADYLP
                     RIPAALEIKDARAAVPLVEAIRAAGIEERVHVTSFMWPALLDAKGSHDGLVYGFLGPR
                     LNRDIIGRSVARGFTQICPHVDSLTKELVDEAHGAGLTVRAWGLSDRGQIERLRASGA
                     DGVTSNWPDWLNET"
BASE COUNT          600 a         1218 c         1284 g          659 t
ORIGIN      
        1 tggcgaaggg ctgggacgtg cgtgttctcg atagcctcga gcgacagacg caccgcaccg
       61 gcaaacccgc ctgggtgccg tccgatgtcg aattcatcca cggcgatgta cgcaatcgct
      121 ccgactggga gcgcgcactg gatggcattg acgtcgtctt ccacgaggcg gcatacggcg
      181 ggtacatgcc cgagatcgcc aagttcatgc acgtcaactc ctatggcacc gccctgatgc
      241 tggagacgat ccggaacaag gaccttcccg ttcgcaagat cgtcgtcgcc tcctcgcagg
      301 cggtttacaa cgaaggcgcc tatagctgcc cccagcacgg cacgttctat ggtgaaacgc
      361 gcccgatcgc ccaactccag gcgggtgact atgcgattca ctgcccggtg tgcggcgcac
      421 catcgacgcc ggtgccgacg gacgaggcgg cgccgatggg cggcgagaat gtctacgcga
      481 tcagcaagtc ggaccaggag cggctggtga tctcgtttgg ccgagcgacc gggatcccgg
      541 tcgtggcgct gcgctactcc tgcacctacg ggccgcgcca atcgctcttc aatccctaca
      601 ccggtgtgat cgccatcttc tgcacccggc tgatcaacga tctggcgccg gtcgtctacg
      661 aggacggtgg ccagtcacgc gatctcgtct ttgtggagga catcgcccgc gccaacctcc
      721 tcgtcgcgac ggacgatcgc gccaatggcc ggatcatgaa tgtgggaacc ggtaacgcgg
      781 cggcaatcgg cgatctcgcg cggctgctcg cgtcgaagct ggggaaggac atcgcgccgc
      841 agatccccgg cgagttccga ccgggagaga tgcgggcgct gatcagcgac atcagccgga
      901 tccaggaact cggcttcgcg ccggtggtcg atctgccgac cggcatcgac cgctacctcg
      961 cctggatcgg tgaacagggc gacatcggcg agtatttctc ggcggcggag cgacgcctgc
     1021 gcgcccggca ggtcgtgaag aaggcccgcg cgtcatgaca tccgaagacc gaccgagacc
     1081 aaccgccgcg atcgtcatcc cctgcctgaa cgaggaggag tcgatcggcg gcctcatcgc
     1141 ggagattctg gccgtcgccg ccgatcccat gctgccaatc gtggtcaatg gaatctatgt
     1201 cgtcgacaac ggcagcaccg atcgcacggc ggatgtcgct cggtcggtcg gggcgaaggt
     1261 cgtctccgag ccgcgtcgtg gctacggacg cgcctgcctc actggagtgc tggcgacggg
     1321 agacgtcgat ctgatcgtgc tgatggatgg cgaccgcagc gacgtgccgg cggagctccc
     1381 gcttctcctc gcgccgatcc tgcggggcga ggcggagctc gtgctcggat cgcgaatgct
     1441 cgggagctac gagccgggct cgctgctgcc gcagcagatc ttcgggaatc gcgtggccgc
     1501 gatcctcttc cggatcgtct atcgcgtgcg catcacggac atcgggccgt tccgcgtcat
     1561 cccccgcgac gcgttgctgg cgctgggaat gcgcgagatg acctatggct ggtcgatcga
     1621 gatgatcgcc cgtgcggttc gcgcgggcct gacggtgcgc gaggtgcctg tgacgtatcg
     1681 caagcgtgcc ggtggcgtct cgaaggtgag cgggaacttc cgggcgtcga tccgggccgg
     1741 ctaccgcatt ctcggggcga tcctccgcgc ccgttcggac aatgcgccat tgccggatga
     1801 cctcgcacgc acgatcggcc ggagccaatg agtcgatcca atcgcctgct gatgatcgcc
     1861 gcccgcgcgc ccattcctgg cgagacgaag acccgcctcg gtcgggcgat cgggatggaa
     1921 caggcggcgc gactctaccg cgccttcctc gtcgatctcg cggcacgttt cacgggcgat
     1981 ggcgagtatg acgtcgcctg gacccactca ccaccggaag cggatttcgc ggcgattcac
     2041 gcggatctct gtggtggagt tcggccgggc gcgcttttcg tgccgcagga aggacccgat
     2101 tgggggaccc ggcaggcaaa tcttctccgc tggggtgtcg atcatggcta cgcccacacg
     2161 atcctgattg cctcggattc gccgcatctc gggggcgaga ccatcgacgc ggcgttcgcc
     2221 gtgctcgagc gcgccgatgt cgcgatcgga cgcgtgcagg atggcggcta ctacctgatc
     2281 ggtctcaatc ggttcgccga tatcctgtcg ggcgtggcga tgagtacgtc gtcggcagcc
     2341 gatggcgtcg tcctccgggc cgggcaactc ggcctgcgcg tggcggaagt tccagccacg
     2401 ttcgacatcg acgaggcatc ggacctgtcc ctcctcgtcg cggcgctggc gccgggcggc
     2461 gacgctgccc cggcgacgtg gcggacgctc gcggcgcttg ggctgcgtga cggcgaacgc
     2521 gtagtgatcc cggagacgac ttcatgacct tcctcaacat cgcgcatcgt ggggcgtccg
     2581 gctatgcccc ggagaatacg cgtgccgcct tcgatctcgc gatcgagatg ggcgccgaca
     2641 tgatcgagac ggatgtgcag ctgacgcggg acgggcagcc cgtcctgatc cacgatggtg
     2701 tggttgaccg cgtctcgaac ggattcggac cgattggtga ttacacgctg gcggagctcg
     2761 aagcgctcga tttcggcgcc tggtacgact cccggttcgc ggggcagcgg atcgtcacgc
     2821 ttgccgtgtt catcgcggat tatctgccgc gaattccggc ggcgctcgag atcaaggatg
     2881 cacgcgccgc ggtcccgctc gtcgaggcga tccgggcggc tggaatcgag gaacgcgttc
     2941 atgtgacgtc gttcatgtgg ccagccctgc tcgacgcgaa ggggagtcat gacgggttgg
     3001 tctacggctt tctcggtcct cgtctgaatc gcgacatcat cggacggagt gtcgctcgtg
     3061 gctttaccca gatctgcccg catgtggact cgctcacgaa ggagcttgtc gacgaagccc
     3121 acggagctgg attgacggtg cgagcgtggg gactctcgga tcgcgggcag atcgaacggt
     3181 tgcgcgcgtc cggcgcggac ggtgtgacca gcaactggcc ggactggctg aacgagacgt
     3241 aggcgcggcg ctcgttgtcg ggcgatcgtg ggtttcgacc cggttgcctc atcccgagcc
     3301 tgtcgaaggg tcggccagac ggctgcatgt ttccacccca acgccgccca ggaacggcga
     3361 caagcgccgt ggggacggga acgagcacat gctcccggca agacccgtcg ccagccgggg
     3421 cggaacgggg agcatcgcgc atgggacgtg gtcccgggcg aacctggtgt tcgcccctac
     3481 ggacgcgcgc ccggctcggg aggacgtgtc gtgcagggcg ctgttcggtg cggtccgcca
     3541 ccacccagac acgtcctccc gagcctgtcg agggatcggt tggcctccgc gtgttcccgc
     3601 tacagctccg tcccatttga acggcgcccg cgggtcggcg acgacggagg gtccggcagg
     3661 catacaaagg ggggatggcc cagcaaccat ccccccgcat cgcgttatcc ctggaatttc
     3721 cctagagcga atcgccctcg gtatcagtgg cgaccgttgc c
//
LOCUS       DASTMD010000010         3689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2590004, whole genome shotgun sequence.
ACCESSION   DASTMD010000010 DASTMD010000000
VERSION     DASTMD010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2590004"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(494..1381)
                     /locus_tag="VFQ54_00135"
     CDS             complement(494..1381)
                     /locus_tag="VFQ54_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU0163414.1"
                     /translation="MNEKPSTKRLPEDLMSGKITALVRGLLPSLVPSEARVARFVLDQ
                     PAEVIHLSVSELGGIANTSASTVMRFCQRLGFTGYQDFKIALAQEAIPPLRRLQADVI
                     EGDTPAAILGKVVHAAGEAVLEAASTIDDATFGRVVEALDEADRILVVGVGSSTPIVQ
                     DIAYRFLTIGLRAEAPMDVHVQHVTASLLGPKDVCLAISHTGSTRETVATTKSAAAAG
                     ARTIALTSFFRSPLTEIAQVVLVTGSKETAFRVEAMASRLAHLAVLDALYVALAVRNR
                     ARAIAAQESYETVLTEHRF"
     gene            1716..>3689
                     /locus_tag="VFQ54_00140"
     CDS             1716..>3689
                     /locus_tag="VFQ54_00140"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEU0163415.1"
                     /translation="MAPRGPMAVLLRSIFILDRTRISLPMAARNTVGFAIPLILLTAI
                     GHPALGVVAGIGTLNVSFSDQAGTDYTKAPRMLAASALAAISVFAGAVLGNYPWIAAI
                     VLALWGFGGGMFAALGVGSMQVGVTALLSYLAMTGNPATPSVALGYGALALAGGVLQT
                     SISILDLSGHRVPPERRAIATAYRALGAYARKLDSPIAGDAPATTEMLAASQLLLERG
                     DDPEGAGGDRMLFDIAQRIQFDLIAIGRVRTATSDIGINLGGVQQALGNLLDVVAGAI
                     SGVGDAGSVDAACGQLRHAIATRATLASIGSGTVDDVGRAIVDDRLAALVTSGELLGA
                     TVTEGDPHDARRDRVRGAVRRWAVRLQESWTTLQANLTLNSAVFRHAIRLAVALVLAQ
                     SIALVLGIDHGYWAPLTIAVILRPDFAGTITRGVARLIGTGIGLVLGTAVVHFLAPGI
                     WEHVIVVTVLTFTIRWVAPSNAVLAAIAISCYIVTLLSMVGIPASTSVADRALNTAFG
                     GVLGLAIFLLWPTWEAAHVRPVLAGMVGSFRRTFRAVMSRPLGREPLMATEIHRLRLR
                     SRLTRTNAAAAVARLMAEPEGDPAQKREAQTLITGMRELAVSTLTLEGYLGAVRGDRV
                     LPQLGPFLNAADGALVWIEGVLDGRDWLSPPPQD"
BASE COUNT          556 a         1207 c         1236 g          690 t
ORIGIN      
        1 taggctcctt tggcctccga cggaattgtt ccattcaccg agttctcacg agactggggc
       61 tgtagtgcgg aaatatacct cccgtcccaa ctgggaacgt ccgggtgacg cgccacgcgt
      121 tcgcatcgtt catcaacgga cacgggtcgg cgaagccgat ccggtacgac acaagggtca
      181 cgtccacggt tcgccataca tcggcgatca aggggtcacg tccatgaaat gaccgtatcg
      241 agctgcgcga tggggtagac cagccgaccc ggtgcgacaa ggggccgtca cacgggacgg
      301 tcgttcgtac cggggggact tcggtgtccc cctttttgcg gctccgagag tacatattgg
      361 tgttcgcctg aaggtcaatt gccgcaatct cgcgaagggc ggccggcctg tcgcctggga
      421 tgagggtggg atcggcaaac gcggtcatgc ttcccacgtt cgttccgtat gtcgtcgatc
      481 ccggggcggg gggctagaac cggtgctctg tgagcacggt ctcgtacgat tcctgggcag
      541 ctatcgcccg cgcgcggttt cgcaccgcca gcgccacgta gagcgcgtcg agcaccgcga
      601 gatgggcgag acgacttgcc atcgcctcga cgcgaaaggc ggtctccttg ctgccggtca
      661 ccagcacgac ctgggcgatc tcggtgagtg gtgaacggaa gaagctcgtc agtgcgatcg
      721 tcctggcgcc ggcggcggcg gctgacttcg tggtggcgac ggtctcccgc gtcgatccag
      781 tgtgactgat cgcgaggcag acatctttcg ggccgagcag gctcgcggtg acgtgctgca
      841 cgtggacatc catcggcgcc tcggcgcgca atccgatggt caggaaccga taggcgatgt
      901 cctggacgat cggcgtcgaa ctcccgacgc caaccacgag aatccgatct gcctcatcca
      961 gcgcctccac gacgcggccg aaggtcgcgt catcgatcgt cgaggccgcc tcgagcaccg
     1021 cttcgccggc ggcgtgaacg accttcccga gaatcgccgc gggcgtatcc ccttcgataa
     1081 catcggcctg cagccggcgg agcggcggta tcgcctcctg ggcgagggcg atcttgaaat
     1141 cctgataacc ggtgaaaccc aaccgctggc agaagcgcat cacggtcgag gccgaggtat
     1201 tcgcgatccc cccgagttcc gagacggaga ggtgaatcac ctcggcgggc tgatcgagga
     1261 cgaatcgcgc cacacgggcc tcgctcggta cgagtgacgg caggagcccg cggacgagcg
     1321 cggtgatctt gcccgacatc agatcttcgg gcaggcgttt tgtcgacggc ttctcgttca
     1381 cgctggtccc catcctcgct attatttccc gttgcggaat cttataccat tgcgctgtac
     1441 caggaagtaa ccggctgacc tggctttgct atgcagtcga gatcatttcc cggcctcgct
     1501 tgtcggcagg tcgggatctg gtccttccat ttaccggccg ttgcatccgg tcgcgatgct
     1561 cggcgctgga tatcgaggcg gagcggggat cgagtggcat aatgagcccg ttctgggcgc
     1621 acgcgtgacc agggcccggc gaatcgaccc tccgatcctg gaacggtgac gtgcaactcc
     1681 ccgcgagtac ccctccccgg gccgggaaat cccggttggc gccacgtggg ccgatggcgg
     1741 tcctgctgcg atcgatcttc atcctcgatc gcacgcggat ttccctgccg atggcggcgc
     1801 gcaacacggt cggtttcgcg atcccgttga tcctgctgac ggcgatcgga caccccgcgc
     1861 ttggtgtcgt cgcgggcatc ggcacgctca atgtctcctt ctccgatcag gccggcaccg
     1921 actacacgaa ggcgccgcgc atgctggctg cgtccgccct cgctgcgatc tccgtgtttg
     1981 caggcgcggt cctcggcaat tacccgtgga tcgcggcaat cgtccttgcc ctgtggggat
     2041 ttggcggcgg catgttcgcg gcgctcggcg tgggctcgat gcaggtcggg gtgacggcgc
     2101 tgctctccta tctcgcgatg accgggaatc ctgccacgcc gtccgtggcg ctcggatatg
     2161 gcgcgctcgc cctggccggc ggtgtcctcc agacgtcgat ctccatcctt gatctctccg
     2221 gtcaccgggt tcccccggaa cgacgggcga tcgcgactgc ctaccgtgcc ctcggcgcgt
     2281 acgcccgcaa actcgattcg ccgatcgccg gggacgcgcc cgcgacgacc gagatgcttg
     2341 ccgcgtcgca gctgctcctt gaacgtgggg atgatcccga gggagccggt ggtgaccgga
     2401 tgctcttcga tatcgcacag cgcatccagt tcgacctgat tgcgatcgga cgcgtgagga
     2461 cggcgacgag cgacatcgga atcaacctcg gcggtgtcca gcaggcgctt gggaacttgc
     2521 tcgacgtcgt tgccggagcg atctcgggtg tgggggacgc cgggagcgtc gacgcggcgt
     2581 gcggccagct gcgtcacgcg atcgcaaccc gggcgacgct cgcgtccatc gggtcgggaa
     2641 cggtcgacga cgtcggtcgt gcgatcgtgg atgatcgcct cgccgcgctc gtcacgagcg
     2701 gtgaactcct cggggcgacg gtcaccgagg gagatccgca cgatgcgcgc cgagaccgcg
     2761 tgcgcggcgc tgtacggcgc tgggctgtgc gactccagga atcctggacg acgctccagg
     2821 ccaacctgac cttgaactcg gccgtctttc gccatgcgat ccggctcgcc gtcgcgctgg
     2881 tgctggcaca gtcgatcgcg ctcgtcctcg ggatcgatca cgggtattgg gcaccactga
     2941 cgatcgcagt catcctgcga ccggacttcg ccggcacgat cacgcgtggc gtcgcccgtc
     3001 tgatcggcac cgggatcgga ctggttcttg gcaccgcggt cgtgcacttc ctggctcccg
     3061 ggatctggga gcacgtgatc gtggtgaccg tgctgacgtt caccattcgt tgggtcgcgc
     3121 cctcgaacgc ggtcctcgcg gcgatcgcga tctcgtgtta catcgtgacg ctgctttcga
     3181 tggtcggcat cccagcgtcg acctccgtgg ccgatcgtgc cctgaacacg gcctttggcg
     3241 gcgtgctcgg actggcgatc ttcctgctct ggccgacgtg ggaggcggcg catgtgcgcc
     3301 cggtgctggc gggcatggtt ggatccttcc gccggacgtt tcgcgcggtc atgagtcgcc
     3361 cgctcgggag ggaaccgctg atggcgacgg agattcaccg tctgcgcctg cgatcccggc
     3421 tgacgcgcac gaacgccgcc gcggcggtcg cgcggctgat ggcggagccg gagggtgatc
     3481 cggcccagaa gcgcgaggcg caaacgctca tcaccggcat gcgcgaactc gcggtctcga
     3541 cgctgacgct cgaaggctat ctcggtgcgg tccgcggcga tcgggttctt ccgcagctcg
     3601 ggccgttcct gaacgccgcc gacggcgcgc tcgtctggat cgaaggcgtc ctcgacggtc
     3661 gcgactggct ttcaccgccg cctcaggac
//
LOCUS       DASTMD010000011         1696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1875516, whole genome shotgun sequence.
ACCESSION   DASTMD010000011 DASTMD010000000
VERSION     DASTMD010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875516"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="VFQ54_00145"
     CDS             <1..1267
                     /locus_tag="VFQ54_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0163416.1"
                     /translation="LATGAIQTIGSENEGIRFWQWDGAGTGKALLLYVSTHYSVGGEF
                     AIHDLADGARTPLTTATTWDLALDYGDGAAWIDATHLLIGGYARSASGLWILDTASGA
                     IENIALADTYEVGLVTRPGSPYVVRAWGGADGVGELIVHNIKTRTSTRITHLNDDYFR
                     ETPPATVERMQARSDGLDVEFLLTKPADFDPAKRYPVVAFVHGGPHMFHHFAYTDSAQ
                     LLASQGYLVVSPNPRGSISYGAEFAAGCVGDWGGGDWRDIVASLDKALELPYADPERT
                     AIYGYSYGGYMSSWAIGQTTRFKAAVIGAPITDLISRYGTSDIGYPIGNVEYDGEFPA
                     LHDWAVERSPMTHVHKATTPTLILHPEEDHRCPIGQGEQLFIGLLKANVETEFIRYPG
                     QSHLMPWGGPSAYRVDFHTRVLAWMAAHL"
BASE COUNT          306 a          578 c          537 g          275 t
ORIGIN      
        1 tctcgcgacc ggcgccatcc agacgatcgg gtccgagaat gaaggcatcc ggttctggca
       61 gtgggacggt gcggggacgg gcaaggcgct cctgctctac gtctcgacgc attactcggt
      121 cggcggcgag ttcgcgatcc acgaccttgc cgatggcgcg cggacgccgc tgaccacggc
      181 gaccacgtgg gatctcgcgc tcgactacgg cgacggtgcg gcgtggatcg acgccaccca
      241 cctcctgatc ggcggctacg cgcgatcggc aagcggtctc tggatcctcg atacggcgtc
      301 cggcgcgatc gagaacatcg cactcgccga tacctatgaa gtcggcctcg tcacccggcc
      361 cggctcccca tacgtcgtgc gagcgtgggg aggcgccgac ggagtgggcg aactgatcgt
      421 ccacaacatc aagacgcgga cgtcgacgcg gatcacgcac ctcaacgacg actacttccg
      481 cgagacgccc ccggcgaccg tcgagcgcat gcaagccagg agcgacggcc tcgatgtcga
      541 gttcctgctg acgaaaccgg ccgacttcga tccggcgaag cgctatccgg tcgtcgcctt
      601 cgtccacggc ggaccgcata tgttccacca cttcgcctac accgattccg cccagctcct
      661 cgcgagccag ggatatctcg tcgtcagtcc gaatccgcgc ggttcgattt cctacggcgc
      721 ggaattcgcc gcgggctgcg tgggggactg gggcggtggc gactggcgcg acatcgtcgc
      781 ctcgctggac aaggcgctcg agttgccata cgccgatccc gagcggacgg cgatctacgg
      841 ctattcctac ggcggctaca tgtcctcgtg ggcgatcggg cagacgacgc ggttcaaggc
      901 ggccgtgatc ggcgccccga tcaccgatct catctcgcgt tacgggacat cggacatcgg
      961 ctatccgatc ggcaacgtcg agtacgacgg cgagttcccg gccctgcacg actgggcggt
     1021 ggagcgctcg ccgatgaccc acgtccacaa ggcgacgacg ccgacgctga tcctgcaccc
     1081 ggaggaagac catcgctgcc cgatcggtca gggcgagcag ctcttcatcg gcctgttgaa
     1141 ggcgaacgtg gagacggagt tcatccgtta tccggggcag tcccacctga tgccgtgggg
     1201 tggaccgagc gcctaccgcg tcgacttcca cacccgcgtc ctcgcgtgga tggcggcgca
     1261 cctgtaactg aacggcacgc ggcgggacgg gccgtcccac ggcccacacg gtggtcgagg
     1321 agggagaggc gggacattcc tccgtccaaa gaccgggcat ccatcgggca agaaatcctg
     1381 atcgccggtc tttggactcc gtagtccaat gccgccccac gtacgaccgc tgcatgtgcg
     1441 acgccaccca cgcaaggtgg cccatacccg gctccgatcc gatcagggaa cacgaggaga
     1501 ggcgagccgt ccgaccctat gaatagttcg tcatatccca gcaatgtcgt cacgcagttc
     1561 atcaccagga gcatgccagg atgggatccg caacacaggc aatcgcattg agccccgaac
     1621 gattggtcta cgatttcaag cgcgccggga gcccgcaact ctcccccgac ggtcatcggg
     1681 tcgtctacgt cgtctc
//
LOCUS       DASTMD010000012         4096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1964891, whole genome shotgun sequence.
ACCESSION   DASTMD010000012 DASTMD010000000
VERSION     DASTMD010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964891"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /gene="ahcY"
                     /locus_tag="VFQ54_00150"
     CDS             <1..1306
                     /gene="ahcY"
                     /locus_tag="VFQ54_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU0163417.1"
                     /translation="LPSNEEKVFMASTTPSARNFDIKDPSLAPAGRRRIEWAAREMPV
                     LAQIRERFEREKPLAGIRLLACAHITTETANLALTLKAGGADAVLCASNPLSTQDDVA
                     AALVEEGIPVFAIKGEDDVTYHGHIQSAIKHAPQLVIDDGADVVTMLHRDYPELIADI
                     IGGTEETTTGVIRERAMEAAGFLKFPIVAVNDADTKHFFDNRYGTGQSTIDGILRATN
                     ILLAGKTIVVAGYGWCGKGIASRVRGLGALVTVTEIDPIRALEAAMDGFVVKKMEDAV
                     ETADFIITATGDINVVDREHFLKAKDGVILANSGHFNSEINLKALSELSGEARRTLRP
                     FVEEYTFDGKSVIVLGEGRLINLAAAEGHPASVMDMSFANQALAAEYIVKEGRNLEPR
                     VYKVPEDIDREIAALKLAAMNITIDVLSAEQQHYLTSWESGT"
     gene            1368..2603
                     /gene="metK"
                     /locus_tag="VFQ54_00155"
     CDS             1368..2603
                     /gene="metK"
                     /locus_tag="VFQ54_00155"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU0163418.1"
                     /translation="MATTFSTAKKLLFTSESVTEGHPDKVCDQISDAVLDAVLRDDPN
                     GRVACETSTTTGLVLVFGEITTSSYVDIQALVREVIADIGYTDSAHGFDAATAGVIVA
                     LKEQSQEISDGVTKALEVRDSAEGGETYDTTGAGDQGMMIGFAANETEELMPLPISLA
                     HKIARRLAEVRKSGEIPYLLPDGKSQVTVEYADGKPVRVDTIVVSTQHLEYATNEQIA
                     KDVEALVVRPVVPAELIDEKTNFYINPSGRFVLGGPAADAGLTGRKIIVDTYGGMARH
                     GGGAFSGKDATKVDRSGAYAARYVAKNVVAAGLADRFELQISYAIGKAHPVSIMCETF
                     GTGKISDDEIERLVRENFDLRPAAIIEHLDLKRPIYRQTAAYGHFGRNDLDLPWERTD
                     KAEALAAAAGLTRDAVPAD"
     gene            2792..3148
                     /locus_tag="VFQ54_00160"
     CDS             2792..3148
                     /locus_tag="VFQ54_00160"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEU0163419.1"
                     /translation="MKLVIAIVQDYDVDKLLRSVTEVGMRATRLSSTGGFLRTGNTTI
                     IMGIDNHRVKECLAIIQRSCSTRVDVRVDASAPEFAEWAAAGVHDVTIGGAVVFIVAV
                     ERFERIGLPAEGGDNA"
     gene            complement(3469..3735)
                     /locus_tag="VFQ54_00165"
     CDS             complement(3469..3735)
                     /locus_tag="VFQ54_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEU0163420.1"
                     /translation="MNSSMISKIEKAHRYAQEPERVSVAAFQATFHGGHDDYQVSLAG
                     DNWACSCHTFDSLGTCSHVMAIQQMLGIMLPEDSRFATPAILAG"
BASE COUNT          782 a         1266 c         1322 g          726 t
ORIGIN      
        1 tctgccgagt aacgaggaga aggtcttcat ggcgtcaact acgccgagtg cacgtaactt
       61 cgacatcaag gatccgtcgc tggcacccgc cggtcgtcgt cgcatcgagt gggcggcccg
      121 cgagatgccg gtactggccc agatccgcga gcgcttcgag cgcgagaagc cgctggccgg
      181 cattcgcctt ctcgcctgcg cgcacatcac gacggagacc gcgaatctcg cgctgacgct
      241 gaaggcgggt ggcgcggatg cggttctctg cgcgagcaat ccgctctcga cccaggacga
      301 tgtcgccgct gcactggtcg aagagggcat tcccgtcttc gcgatcaagg gcgaggacga
      361 cgttacctat cacggccaca tccagagcgc gatcaagcac gcaccccagc tcgtgatcga
      421 cgacggggcg gacgtggtga cgatgctgca ccgggactac ccggagctga tcgccgacat
      481 catcggcggc acggaagaga cgacgacggg cgtcattcgc gagcgcgcga tggaggccgc
      541 cggcttcctc aagttcccga tcgtcgccgt caatgacgcc gacaccaagc acttcttcga
      601 caaccggtac ggcaccggcc agagcacgat cgacggcatt ctccgcgcca cgaacatcct
      661 gctcgccggc aagaccatcg tcgtcgccgg ctatggctgg tgcggcaagg gtatcgccag
      721 ccgcgttcgc gggctcggcg cactcgtcac ggtgacggag atcgatccga tccgcgccct
      781 cgaggcggcg atggatggct tcgtggtgaa gaagatggaa gatgccgtgg agacggccga
      841 tttcatcatc acggcaaccg gcgacatcaa tgtcgtcgac agggagcatt tcctgaaggc
      901 gaaggacggc gtgattctcg ccaactccgg gcacttcaat tcggagatca acctgaaggc
      961 gctctccgag ctttccggtg aggctcgccg cacgctccgc ccgttcgtcg aggaatatac
     1021 gttcgacggc aagagcgtga tcgtgcttgg cgaaggtcgc ctgatcaacc tcgccgcggc
     1081 ggaaggccat ccggcgagcg tgatggacat gagcttcgcg aaccaggcgc tggccgcgga
     1141 gtatattgtc aaggaaggcc gtaatctcga gccgcgcgtc tacaaggtgc cggaggacat
     1201 cgatcgcgag atcgccgccc tcaagcttgc cgcgatgaac atcacgatcg atgtcctctc
     1261 ggcagagcag cagcactacc tgacgtcgtg ggaatccggc acctagttcg accggtcgca
     1321 ggtgaatctc gtcgcccttt ccggcagtcg cccaacgagg aatcgaaatg gccacaacat
     1381 tcagtacagc gaagaaactg ctcttcacct ccgagtccgt gacggaggga catccggaca
     1441 aggtctgcga ccagatctcc gatgccgtcc tggacgccgt tcttcgagat gatccgaatg
     1501 gccgcgtcgc gtgcgagaca tcgacgacga ccgggctcgt gctcgtcttc ggtgagatca
     1561 cgacgtccag ctacgtcgac atccaggcgc tggtgcgtga ggtcatcgcc gatatcggat
     1621 acaccgattc ggcgcacggt ttcgatgcgg cgacggcggg cgtgatcgtc gccctcaagg
     1681 aacagagcca ggaaatctcg gatggcgtca ccaaggcgct cgaagtccgt gattcggccg
     1741 aaggcggcga gacgtacgac accaccggcg ccggtgacca gggcatgatg atcggtttcg
     1801 cggcgaacga gacggaagag ctgatgccgc tgccgatctc gctggcgcac aagatcgcgc
     1861 gtcgcctcgc cgaggtgcgc aagagcggcg agattcctta cctcctgccc gatggcaaga
     1921 gccaggtgac ggtcgagtat gcggacggca agccggtgcg cgtcgacacg atcgtcgtct
     1981 cgacccagca tctcgagtac gcgacgaacg agcagatcgc aaaggacgtc gaggcactcg
     2041 tcgttcggcc ggtcgttccc gccgaactga tcgatgagaa gaccaacttc tacatcaatc
     2101 cgagcggacg cttcgtgctg ggtggaccgg cggcggacgc cggcctgacc ggtcgcaaga
     2161 tcatcgtcga cacctacggc ggtatggccc gccacggtgg tggcgccttc tccggcaagg
     2221 atgcgacgaa ggtcgatcgc tccggcgcct acgcggcgcg ctacgtcgcg aagaacgtcg
     2281 tggcagcggg tctcgccgat cgcttcgagc tgcagatctc ctacgcgatc ggaaaggccc
     2341 atccggtctc gatcatgtgc gagaccttcg gcacgggcaa gatctcggac gacgagatcg
     2401 agcgcctcgt gcgcgagaac ttcgatctcc gcccggcggc gatcatcgag catctcgacc
     2461 tcaagcgacc gatctaccgc cagacggcgg cctacggtca cttcggacgt aacgatctcg
     2521 atctcccctg ggagcgaacc gacaaggcgg aggcgctcgc cgcggccgcc ggtctcaccc
     2581 gggatgctgt cccggcagac tagcttgttg gccccggcgt gaccttcagg ggcgtcgacc
     2641 cggaatcggc atggatgaat acggacctcc ataatttcat ggaggtccgt attcggcttt
     2701 cgccggggtg cgatccctgg ccccacaccc acgtcgcctg tgctataacg gcgcaacgac
     2761 ttcgcgcgtt cggtgggatt atctctcttc catgaaacta gtgattgcaa tcgttcagga
     2821 ctatgacgtc gacaaacttc tgcggtcggt cacggaagtc gggatgcggg ctacgcgcct
     2881 ctccagcacc gggggattcc tccgtaccgg gaatacgacc atcatcatgg ggatcgacaa
     2941 tcatcgggtg aaagaatgcc tcgcgatcat tcagcgctcc tgctcgacgc gcgtggatgt
     3001 gcgtgtggat gcatcggcac cggagtttgc cgagtgggcg gcggcgggag ttcatgacgt
     3061 gacgatcggc ggcgccgtgg tcttcatcgt cgccgtcgag cgattcgaac gtatcggtct
     3121 tccggccgaa gggggcgaca atgcatgacg accacccaca cggcccgatc gaccggcttc
     3181 gcgcctatgg cagggcgctg gtgatcgcgg ccggcgtctc attcggtcaa cttggagaca
     3241 catcgcgagg accccaccgg ccgccgaccg ccatcgtgca ccttcttccg gagcgaaagg
     3301 atgcattcga ccggctggag accgttgagg cggcggtgct gacacagcga ctggtcgtgg
     3361 gcgaggaatc ggaacccaga cggttcaccg agtcgcccaa ctgacgctac cgggcgtcac
     3421 gacgggcgag atgcgtcttc cgtccgtcgt gacgccgcga ggcggaggct aaccggcgag
     3481 aatcgcgggc gtggcgaatc gggaatcctc cgggagcatg atccccagca tctgctggat
     3541 ggccatgaca tggctgcagg tgccaaggct gtcgaacgta tggcaggagc atgcccagtt
     3601 gtccccggcg agggagacct ggtaatcatc gtggccaccg tggaaggttg cctggaaagc
     3661 ggcgacggag acccgttccg gttcctgagc gtaccgatgt gctttctcga tcttgctgat
     3721 catgcttgaa ttcatcgagg tcgcgctcct tttcctgcgg tgtatgaccg cttcgaaccg
     3781 ggcgacccgg tgctcatggt tgtcgactgc gccggaagtc acgtcgctcg tttccagcga
     3841 gcgagaatga atcgaacggt gcgaacgcaa ccgggaccga aaacaaaaaa gcccggcagg
     3901 ctcggcctgg cggtgcttcg gtggtcggtc ttgcgttgtt ccgatcgtga tccatggcgt
     3961 aggcgcccct gagtcggtat cgaccacacc catggtcgat acgtgtccgg atcgtcgttg
     4021 tagcggaagt atacccagcc tggtcgccga atgctacggg gtacttcata tttgcgtggg
     4081 gcggaagaga gatgac
//
LOCUS       DASTMD010000013         4045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2322054, whole genome shotgun sequence.
ACCESSION   DASTMD010000013 DASTMD010000000
VERSION     DASTMD010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2322054"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFQ54_00170"
     CDS             complement(<1..546)
                     /locus_tag="VFQ54_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEU0163421.1"
                     /translation="MSDLLITGGRIVDGTGNPWFYGDVAVTGDIIERIAPPGTIDPGT
                     STTVIDATDHVVSPGFIDIQSHSIRTFFTDRRSLSKVTQGVTTEIMGELWTPAPVGGQ
                     ILTALDSSLAERGDEDIPEWVELAKGWSRFGDWLAELEKRTVSVNFGSFLGGGTLREY
                     ACGLRMGDATEDEIAVMQRAVA"
     gene            complement(543..2192)
                     /locus_tag="VFQ54_00175"
     CDS             complement(543..2192)
                     /locus_tag="VFQ54_00175"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEU0163422.1"
                     /translation="MVDTLIVGGRIVDGTGNPWFYGDVAIAGDRIERIAPPGTIDPAT
                     ATTVVDATDHVVSPGFIDIQSHSIIPWLTDGRSLSKVTQGVTTEILGEGWTPAPFGGK
                     LESAFSGALVHRIGEDQAKEWETLGKTWSRFGDWLAEFEGRGISVNFGSFLGGGTVRE
                     FGCGERMGEATDEEVAAMTQMIAESMEDGALGIATALIYPPNAYSKTPELIACMEVVA
                     RYNGVHITHMRSEGDLIFDGLAETLDIAEATSVATEIYHLKASGRPNWEKMAGVIHRI
                     NEARANGIDVTADMYPYDGSGTGLATCLPYWVSENGKRHENLRDPETRTRILAEMRSE
                     DTEWENMGLRTGPENIILADLKKPENIRFRGWRLSQIAEELGLDWPETIVHLLDTEGT
                     NIFTMYLGMSDDNIQVQVKQPWIKFSTDAGGVDPELVADLGLVHPRAYGTYTRVLGRF
                     VREYGWLTLEDAVRKSSSAVADRLGLRDRGLLRDGLKADVIVFDPETVIDRATYTDPH
                     HLSIGIRDVFVNGTAVLRDSEHTGAFPGQRVNGPGYTGVEA"
     gene            2572..>4045
                     /locus_tag="VFQ54_00180"
     CDS             2572..>4045
                     /locus_tag="VFQ54_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163423.1"
                     /translation="MTEQDARATSFLKSRLTRRTLGAAALAGIVPAVALAQATPNASP
                     VASPAASPAASPAASPAVASPVASPASSPIASPVASPAASPVAARTTDSWFLTSAQQD
                     GGGFTITADIFDVYSGTTREKLGGFHAPGTTDMCVTSDPGKVLLTTSGGVGVFDLTAG
                     KFSVLDGTAGVNPSNIWLPDPRIFQQTPAGWAFVRTYDASQAWVVDLTSLTATDIVPI
                     VETDGGTKSFANIAFSEDGSVAAAYVDDRGVAIFDPADPSGARRLDGGESGAVAGLPV
                     FSDSGEYIAYPLLDSAASNEAKIIVEELSSRAIVATIGGVSATASVLFVPNSDVDLLI
                     MDSGTISRQAIDNGAETWSVDARNYAYGWGYTNDLKHILYGSVDTIGGDIYWDVISLA
                     DGTRTSLDGLEALTFYNGSFPTGGSYTLFGPPWLSPSEDNKDALVGFNSDDKKVTPLL
                     DDSSAWALSTGYATSVDGRIAMYSDVDTSLLNLETGQVRNF"
BASE COUNT          672 a         1283 c         1320 g          770 t
ORIGIN      
        1 ggcaactgca cgctgcatga ccgcgatctc gtcttcggtt gcatcgccca tccgtaggcc
       61 gcaggcgtat tcgcgcagcg ttccgccacc gaggaaggat ccgaagttca ccgagaccgt
      121 gcgcttctcg agttccgcca gccagtcgcc gaagcgggac cagcccttgg cgagttcgac
      181 ccactccgga atgtcttcgt ccccgcgctc agccagcgag ctgtcgagcg cggtgaggat
      241 ctgcccaccg accggagcag gcgtccagag ttcgcccatg atctccgtcg tcacgccctg
      301 ggtgactttc gaaagggagc ggcgatcggt gaagaaggtc ctgatcgagt gcgactggat
      361 gtcgatgaag ccgggcgaga cgacgtgatc cgtggcatcg atgacggtgg tcgacgtgcc
      421 gggatcgatc gtgcctggcg gggcgatccg ctcgatgatg tcgcccgtga cagcgacatc
      481 gccgtagaac cagggattgc cggtgccatc gacgatcctc ccgccggtaa tcagcaggtc
      541 actcatgctt cgactcctgt gtagccgggt ccgttgacgc gctgtccggg gaaggcgccg
      601 gtgtgctcgc tgtcgcggag gacggccgtg ccgttcacga agacatcacg gataccgatc
      661 gagaggtgat ggggatcggt gtaggtcgcg cggtcgatca cggtctctgg atcgaagacg
      721 atcacgtccg ccttcaggcc gtcgcgcagg agcccgcgat cgcggagccc gaggcggtcg
      781 gcgacggcgg agctcgactt gcggaccgcg tcttccagcg tcagccagcc atattcgcgg
      841 acgaagcgcc cgagtacccg cgtataggtc ccgtaggcgc gcggatggac gagtccgaga
      901 tcggcgacga gttcggggtc gacgccgccg gcatcggtcg agaacttgat ccatggctgc
      961 ttgacctgga cctggatgtt gtcgtcgctc atcccgagat acatcgtgaa gatgttggtg
     1021 ccttcggtgt cgagcaggtg gacgatcgtt tccggccagt cgagtccgag ttcctccgcg
     1081 atctgggaga gccgccagcc gcggaagcgg atgttctccg gcttcttgag atcggcaagg
     1141 atgatgttct cggggccggt gcgaagaccc atgttttccc actcggtgtc ttcgctgcgc
     1201 atctcggcga gaatccgcgt tcgagtctcg gggtcccgga gattctcgtg gcgcttgccg
     1261 ttctcggaga cccagtaggg aaggcaggtc gcgagaccgg tgccggaacc gtcgtaggga
     1321 tacatgtctg ccgtgacgtc gatcccgttg gcacgggcct cgttgatccg gtggatgacg
     1381 cccgccatct tctcccagtt cggccggccg gatgccttga gatgatagat ctcggtcgcg
     1441 acgctggtcg cctcggcgat gtcgagcgtt tcggcgagcc cgtcgaagat gagatcgcct
     1501 tccgagcgca tatgcgtgat gtggacgccg ttgtacctcg cgacgacctc catgcaggca
     1561 atcagctcag gcgtcttcga ataggcgttc ggcggataga tgagtgccgt cgcgatccct
     1621 aaggcgccgt cttccatcga ttcggcgatc atctgcgtca tcgccgcgac ttcttcgtcg
     1681 gtcgcctcgc ccatgcgctc gccgcagccg aattcgcgga cggtgccgcc gccgaggaag
     1741 gagccgaagt tgaccgagat accgcgtccc tcgaactcgg cgagccagtc accgaagcgg
     1801 gaccacgtct tgccgagcgt ctcccactcc ttcgcctgat cttcaccgat gcggtggacg
     1861 agggcccccg agaaggcgct ttcgagcttt ccaccgaagg gagccggagt ccatccctcg
     1921 ccgaggatct ccgtcgtcac gccctgcgtg accttcgaga gggaacgccc gtccgtcagc
     1981 cacgggatga tcgaatggga ctggatgtcg atgaagccgg gggagacgac gtgatccgtc
     2041 gcgtcgacca ccgtggtggc cgtcgcggga tcgatcgtgc ccggtggtgc gatgcgttcg
     2101 atccgatccc cggcgatggc cacatcgccg tagaaccagg ggttccccgt tccgtcgacg
     2161 attcgcccgc ccacgatcag cgtatcgacc atcgtgtttc cactccttga tatgcgccgg
     2221 ctggattgct ttgggctcgt ccgagcccgt gtctcgcccg cgatcatacc ccgggatttc
     2281 cgatcgcggt tcaaacccgt cccgggcgat gacgcggatt tgccgatttg gtgaacgtaa
     2341 ttgcgcttcc cgacgttgtt gatgacaaag gtttgacggt cgatttgtga tcgctgcgca
     2401 ggatgcagag attgggtaac ccgacctgac agatgatcca tgacggccgt tgtgttgcta
     2461 ctcttgtggc gagggatgat tctgatccaa agccgcaacc atccggaatc gatcgactga
     2521 cagcaaccag tgtttcgaca cgatgcgata tcaccttggg gagagcactc gatgacagaa
     2581 caggatgcgc gggctaccag tttcctgaag agccggctca cgcggaggac gctgggagcg
     2641 gctgcgctgg ccggaatcgt gccggcggtg gccctggcgc aggccacgcc gaacgcgtca
     2701 cccgtcgcct ccccggcggc gagtcccgcc gcgtcgcccg cggccagtcc tgccgtcgct
     2761 tcgccagtcg cgagtccggc ttcctcgccg atcgctagcc cggtcgcgtc tcccgccgct
     2821 tctcccgtcg ccgcgcggac gaccgactcc tggttcctga cctctgcgca gcaggacggt
     2881 ggcggattca ccatcactgc cgacatcttc gatgtctatt ccgggacgac ccgggagaag
     2941 cttggcggct tccacgcgcc gggcacgacg gacatgtgcg tgacctccga tcccgggaag
     3001 gtgctgctca ccacctcggg cggcgtcggc gtcttcgatc tgacagccgg caagttctcg
     3061 gtgctggatg gcaccgccgg cgtcaaccct tccaacatct ggctccccga tccgcggatc
     3121 ttccagcaga cgccggccgg gtgggcgttc gtgcgcacct acgatgcctc gcaggcgtgg
     3181 gtcgtcgatc tgacgagcct gacggcgacc gacatcgtgc cgatcgtcga gacggacggg
     3241 gggacgaaat ccttcgccaa catcgccttc tcggaggatg ggagtgtcgc cgccgcctat
     3301 gtcgatgacc gtggcgtggc gatcttcgac ccggcggatc catccggagc ccgccggctc
     3361 gacggtggtg agtccggcgc cgtcgcgggg ctgccggttt tctccgattc cggcgagtac
     3421 atcgcctacc cgttgctcga cagcgcggct tcgaacgagg cgaagatcat cgtcgaggaa
     3481 ctctcctcgc gggcgatcgt ggcgacgatt ggcggcgtca gcgcgacggc atcggtgctc
     3541 ttcgtcccga acagcgacgt cgacctgctg atcatggatt cgggaacgat ctcgcgacag
     3601 gcgatcgaca acggcgccga gacgtggagc gtcgatgcca ggaactacgc ctatggctgg
     3661 ggatacacga acgatctcaa gcacatcctg tacggatcgg tcgatacgat cggcggagac
     3721 atctactggg acgtgatctc gctcgcggat gggaccagga ccagtctcga tggcctcgag
     3781 gcgctgacct tctacaacgg cagcttcccg accggcggct cctacacgct cttcggtccg
     3841 ccgtggctct ccccgagcga ggacaacaag gacgccctgg tcggcttcaa cagcgacgac
     3901 aagaaggtga cgccgcttct tgatgattcc agtgcctggg cgctgagcac cggctacgcg
     3961 acctcggtgg atggcaggat cgccatgtat tcggatgtcg acacgtcgct gttgaatctg
     4021 gaaacagggc aggtccggaa cttcg
//
LOCUS       DASTMD010000014         1884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_536811, whole genome shotgun sequence.
ACCESSION   DASTMD010000014 DASTMD010000000
VERSION     DASTMD010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536811"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VFQ54_00185"
     CDS             <1..860
                     /locus_tag="VFQ54_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0163424.1"
                     /translation="QADAFIWESGGRGIDGRPEIRLGLKGICYVELRAKGARHDWHSS
                     VATTVPNPAWRLVWALRSLKNIDERVQIPGFYDSVIGPTERELEVLVDIPNTEEARLK
                     NLGIDQFLGGRTGVELAAWDYFQPTCTISGFESGYHEAGMKTVLPATATAKLDMRLVA
                     NQDPFIIYDLLRKHLDDNGFADIETEPLAQQYPARTRLDAPIAEVVADSYRAVYEQEP
                     VFIPTSLGSGPLYQLCAQFGIDAAPSGAGNARSQAHAPNENIYVDDFILAIKHVTQII
                     ERFAAREEI"
     gene            1375..>1884
                     /locus_tag="VFQ54_00190"
     CDS             1375..>1884
                     /locus_tag="VFQ54_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163425.1"
                     /translation="MCQYGTNHQTASSLGVGRDLANRRSTAFDRRVVLKATAGLAAMS
                     AMGGVSVASGATSVAAQEAGGTRASRWTPEPSVDAKAGPDGDAGSDGFRDFETDFPFY
                     ALGASWDQSVGLWPVIAVQVSSDGDSWSDTFQLVADAEDGGQPTRDNRIFTPLIFTDG
                     VSWVRFQALD"
BASE COUNT          347 a          593 c          607 g          337 t
ORIGIN      
        1 gccaggccga tgccttcatc tgggagtccg gcggacgcgg catcgacggc cggcccgaga
       61 tccggctcgg cctcaagggc atctgctatg tcgaactccg ggcgaagggt gcccggcacg
      121 actggcactc ctctgtcgcg accaccgttc cgaacccggc ctggcggctc gtatgggcgc
      181 tgcgcagcct caagaatatt gacgagcgcg tccagatccc gggcttctac gactccgtga
      241 tcggcccgac cgaacgcgag ctcgaagtgc tggtggatat tccgaacacc gaggaagcgc
      301 gcctcaagaa cctcgggatc gaccagttcc tcggcggacg gacgggagtc gaactggccg
      361 cctgggacta cttccaaccg acatgcacga tctcgggatt cgagagcggc tatcacgagg
      421 cgggcatgaa gacggtgctg cccgccactg cgacggcgaa gctcgacatg cgtctcgtcg
      481 cgaatcagga ccctttcatc atctacgacc ttctgcgcaa gcacctcgat gacaatggct
      541 tcgccgacat cgagaccgaa ccgctggcgc agcagtatcc ggcgcgcacg cggctcgacg
      601 cgccgatcgc cgaagtcgtc gccgattcct atcgcgcggt ttacgagcag gagccggtgt
      661 tcatcccgac ttcgcttggc tcgggcccgc tctaccaact ctgcgcgcag ttcgggatcg
      721 acgccgcgcc gagcggggca ggaaacgccc gttcgcaggc gcatgctccg aacgagaaca
      781 tctacgtcga cgacttcatc ctcgcaatca agcacgtcac gcagatcatc gagcgcttcg
      841 cggctcgcga ggaaatctag tcggcggccc gagggctgag agaagtcctc tcaattggtg
      901 catccactaa atcgtggtcg agatgcgcgt tcgccaaggg gcgacataga agtcgccccg
      961 cactcacgac cggcgtgcgt acggcgtccg cgagtagggc gggacttcta tgtcccacct
     1021 tctggtgtca ccgctctcag gaccagccgg atacgtgcct caattgagct ttcggaagaa
     1081 gtcgatgacg atctcctccg gaccggtcat catgagatcc tggcggatgc gtgcccggtc
     1141 gacaagagca tgtttcgttt cgtaaaggga tcgcaagatg gtgactgtga gtcaccgatc
     1201 ctgatatttc tgtgatattg tgtgtacgta cataggattc ggccagacac gagctgcgag
     1261 tgtacccaac tctcgcctcg gcttccctgg ccggtttcgg ggacaccgag acgcccggcg
     1321 cgatctggct ggagatcgcc gggtatcgga gaagatttct ggggggactc aagcatgtgc
     1381 caatatggaa cgaatcatca gaccgcgagt tcgttgggag tcggtcgaga cctcgcgaac
     1441 cgccggagca cggcgttcga tcggagggtc gtcctcaagg cgacggcggg gctggccgcg
     1501 atgtcggcga tgggaggagt ttccgtcgcc tccggcgcca cgtcggtcgc ggcgcaggaa
     1561 gccggaggca ctcgcgcgag ccggtggact ccggaaccaa gcgtcgacgc gaaggccggg
     1621 ccggacggcg acgcagggag cgacggcttc cgtgacttcg aaaccgactt tcccttctac
     1681 gcgcttggcg cgagctggga ccaatccgtt gggctctggc cggtgatcgc ggtccaggtc
     1741 agcagcgacg gcgactcgtg gtcggacacg ttccaactgg tcgccgatgc cgaggatggc
     1801 ggtcagccga cgcgtgacaa tcggatcttc acgccactga tcttcaccga tggcgtctcc
     1861 tgggtccgct tccaggcgct cgac
//
LOCUS       DASTMD010000015         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2232947, whole genome shotgun sequence.
ACCESSION   DASTMD010000015 DASTMD010000000
VERSION     DASTMD010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232947"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VFQ54_00195"
     CDS             complement(<1..506)
                     /locus_tag="VFQ54_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0163426.1"
                     /translation="MIGTPDPRRWLALWVIAIAQLMVVLDASIVNIALPKAQAALNIS
                     DADRQWVVTAYTLAFGGLLLLGGRIADFVGRKRVFIIGLIGFAIASAIGGLAQNEGML
                     FGARALQGAFAAVLAPAALSLITVTFTEQHERARAFAVYGAISGGGAAIGLILGGVLT
                     EYASWRWCL"
     gene            1096..1668
                     /locus_tag="VFQ54_00200"
     CDS             1096..1668
                     /locus_tag="VFQ54_00200"
                     /inference="COORDINATES: protein motif:HMM:NF037675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEU0163427.1"
                     /translation="MNTRDPDSKRERLLAAARAEFSAHGLAGARIDRIARTAKCSAGL
                     VYTYFGSKDELADAVLVRTIGDALEEAPVTTDDLPGWAGQLFDRNADHPELFRIATWF
                     RLERGAPSHVDGIEESSHAKLAAIEAAQAAGCLPTHFAASELLALVIHLSTLWSAMPG
                     AYEVESGCLSRETQRRVIVDAVAAIIEKSH"
     gene            1783..1947
                     /locus_tag="VFQ54_00205"
     CDS             1783..1947
                     /locus_tag="VFQ54_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163428.1"
                     /translation="MPNTFILVVLTVLLVAAAYGSFRNATSAPPTGSTPAEENDPVYE
                     WDDQDWIGSK"
     gene            complement(1923..>2880)
                     /locus_tag="VFQ54_00210"
     CDS             complement(1923..>2880)
                     /locus_tag="VFQ54_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163429.1"
                     /translation="QLTYRYTAVDIQNRELDPSGTPVLLLASALYMAAEVQAKLVDYL
                     RAGGRLYLQGELPRFDMLGGECRLLLDALELEPVSERFSDHRTFLSVEAGGWAAPLPE
                     LRIGYAQTFQPGPGTVLQLYATEEAVGFDVAVGGGRAIVVTCDLPPDLDFHHRAFGQL
                     GMSPSLTHDHPERNIFLTSTRNERGERFIHALNLDGFDKSIHLEEAGEPLLGGREVML
                     RARDGVMLPLDLDLGEGVRLIWSTAEVVRRRENGFTVRLTGESDELALKVGEELDVVA
                     DDTTEIVRDGSVAYVRILGCSAGEFIDIDWMAAGDHFEPIQS"
BASE COUNT          560 a          952 c          856 g          512 t
ORIGIN      
        1 aggcaccagc gccacgacgc gtactcggtc agcacgccgc caaggatgag cccgatcgcc
       61 gcgccaccac cggagattgc accgtagacg gcgaatgcgc gagcgcgttc gtgctgctcg
      121 gtgaaggtaa ccgtgatcag cgacagagcg gccggggcca gtaccgccgc aaatgcgccc
      181 tgcagggcgc gcgccccgaa gagcatcccc tcgttctggg caagtccacc gatcgccgag
      241 gcgatcgcaa acccgatcag cccgatgatg aagacacgct tgcgaccgac aaagtccgcg
      301 atacgccctc caagcaggag gagaccgccg aacgccagcg tataggccgt gaccacccac
      361 tgccgatcgg catcggagat attcaacgcg gcttgcgctt tcggcagcgc gatattcacg
      421 atcgatgcgt cgagaacgac catcaactgt gcgatggcga tcacccacaa tgcgagccaa
      481 cgcctcggat ccggagttcc tatcatcgag gcggtttctg ttcgccctcg ttccatgacc
      541 aatcccttcc tgacgcgatg tattccatgg tttccgcacc gcaccagaaa acacggtcga
      601 accgcgctca acgcgtacgt tcatctaccg tattcgttac gattcgtaat tcgcaactag
      661 acgaagtacg gggttccaga cgtcacaacg agacgcctat gatcgtcgta acgcagctgg
      721 actgcgccag atcgtgcggc agtcactacg tcgcggacgc ttcgccggcc gtgaaagcga
      781 cgccctggat cggattcggt tgccgaatcg ctgccgggcg tcgtctggat gcgggcctcg
      841 cttcgatgct ctcctgcctc ctcgaggcag aatcgcccgg atctgtgcag atttagcggc
      901 ccccctgggt aatggtgacg ggaatcggcg atacgatggc tgtggtgttg tcagacatcc
      961 cggcatggtc ttcggtctgc cgggatcaag aaccgtcgcc gtctcccaaa cgaacgctca
     1021 ctcagtagcg tcgccccagt taagcactca acgcgacgcc tgtcaaatga acgttcattc
     1081 aactagaatg tcagcatgaa cacgcgtgat cctgattcca aacgggaacg acttctcgcc
     1141 gccgccaggg ccgagttctc ggcgcacggg cttgccggcg cgcggatcga ccgcatcgcc
     1201 agaacggcga aatgcagcgc cgggctcgtc tacacctact ttggcagcaa ggatgagctc
     1261 gcggacgcgg tcctggtgcg caccatcggc gacgctctcg aggaagctcc ggtgacgacc
     1321 gacgacctcc ccggctgggc cggccagctc ttcgatcgca atgcggatca tccggagttg
     1381 tttcgaatcg cgacctggtt tcgcctcgaa cgcggcgcgc catcccatgt cgacggaatc
     1441 gaagagagca gtcacgcgaa actcgcggcg atcgaggcgg ctcaggcagc cgggtgtctc
     1501 cccactcatt tcgccgcctc ggaactcctc gcgctcgtga ttcacctctc gaccctctgg
     1561 tcggcgatgc cgggcgcgta tgaagtcgag tcggggtgtc tctcccgcga gacccaacgc
     1621 cgggtgatcg tcgacgccgt cgccgcgatc atcgagaagt cgcactgatc ggcgaaaccg
     1681 aaagcacgct catcacgttc tcagaaatgg gaagggtatg aacgacggcg cacaacaaca
     1741 cggtgtgtcg tggatatgcg cgtgcatgct tgggagcgtt cgatgccgaa cacattcatc
     1801 ctcgtcgtcc ttaccgttct cctcgtcgcc gcggcgtacg gttccttccg caacgcgaca
     1861 tccgctccgc caaccggatc cacaccggcg gaggagaacg atcccgtcta tgagtgggac
     1921 gatcaggact ggatcggttc aaagtgatcg ccggccgcca tccagtcgat gtcgatgaat
     1981 tcccccgccg agcatcccag tatgcgtacg tacgcaaccg atccgtcgcg cacgatctcc
     2041 gtggtgtcgt ctgcgacgac atcgagctcc tcgcccacct tcagcgccag ttcgtctgac
     2101 tctccggtaa ggcgcaccgt gaagccgttc tcccgtcgcc tgaccacctc ggccgtcgac
     2161 cagatcaggc ggacgccctc tccgagatcc aggtcgagtg gcagcatgac gccgtcccgg
     2221 gcccgaagca tcacttctcg acctcccagt agcggctcgc ccgcctcctc caggtgaatc
     2281 gacttgtcga agccgtcgag attgagcgcg tgtatgaatc gctcgccgcg ctcattgcgt
     2341 gtcgacgtca ggaagatatt tcgctcggga tgatcgtggg tgaggctcgg actcatcccc
     2401 agttgaccga acgcccggtg atggaagtcg agatcaggcg gcaggtcgca agtgaccacg
     2461 atcgcccgcc ccccgccgac ggcgacatcg aagccaaccg cttcttccgt agcgtaaagc
     2521 tgaaggaccg tgccgggtcc aggttggaac gtctgcgcgt agccgatccg aagctcgggc
     2581 agcggggcgg cccacccgcc cgcctcgacc gagagaaagg tccggtggtc ggagaaccgc
     2641 tcgctcaccg gctccagttc gagcgcatcc agcaggagcc ggcattcgcc gccgagcatg
     2701 tcgaagcgcg ggagttcgcc ttgcaggtaa agccttcccc cggcacggag atagtcgacg
     2761 agcttcgcct gaacctccgc cgccatatac agggcggagg caaggagcag aaccggggtt
     2821 ccggatggat cgagctcccg gttctggatg tcgaccgcgg tatatcgata cgtcagttgc
//
LOCUS       DASTMD010000016         6034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2947485, whole genome shotgun sequence.
ACCESSION   DASTMD010000016 DASTMD010000000
VERSION     DASTMD010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2947485"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..139)
                     /locus_tag="VFQ54_00215"
     tRNA            complement(64..139)
                     /locus_tag="VFQ54_00215"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(104..106),aa:Gly,seq:ccc)
     gene            complement(281..808)
                     /locus_tag="VFQ54_00220"
     CDS             complement(281..808)
                     /locus_tag="VFQ54_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163430.1"
                     /translation="MNDRGGHEVSQDGAADILYLVDQLEELVGSGKRVPFSTRVMVEE
                     EEFLTLVDQLREALPNEIKQARRVIKERERIVGQAQEDALKIIQDARVDADRLVSQHG
                     ITIEARQRSEELLRQAEEERQRARGEIDVFIMEQIGLVESSVRRGMALMEDAVEQTLE
                     TIERTRDAVGRDARF"
     gene            complement(949..1464)
                     /gene="coaD"
                     /locus_tag="VFQ54_00225"
     CDS             complement(949..1464)
                     /gene="coaD"
                     /locus_tag="VFQ54_00225"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU0163431.1"
                     /translation="MTLAIYPGSFDPITNGHLDVALRAARLFDGIILSVYEGSEKPTA
                     LFSFAERIALAKEAIRTSTDPYATRIVVESFTGLTVNYAREKGASTIVRGLRAVSDFE
                     YEFKYAHMNEHLAPEIEVVCLMTSAQHSFISSTFIREVSSLGGNVHGLVPANVEEALM
                     ARFAISAPGVR"
     gene            complement(1461..2057)
                     /gene="rsmD"
                     /locus_tag="VFQ54_00230"
     CDS             complement(1461..2057)
                     /gene="rsmD"
                     /locus_tag="VFQ54_00230"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU0163432.1"
                     /translation="MRVISGSARGRQLKGPPSFATRPMTDKLKVALFNSLMSLGIEPD
                     RVLDLYAGTGALGIETLSRGASFVTFVDKGKEQIATIRWNLEHTDFAAKSDVRQIPVD
                     AFIQSARTPYDFIMVDPPYADPQIVPILGQIAESPLVEPGTIVVLGHADRVEVPDRVG
                     NLERLRHRCHGDSCFSIYEFAIDDASQAGTEPATGEDR"
     gene            complement(2151..4652)
                     /gene="recG"
                     /locus_tag="VFQ54_00235"
     CDS             complement(2151..4652)
                     /gene="recG"
                     /locus_tag="VFQ54_00235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU0163433.1"
                     /translation="MTLRSTDVSRNTNEQRFERMLDALRRAWKGGPALQRDILLAVRM
                     LRELQARVASTHEVGGSIVTAIATLEHLPANESVRREQIQQVATVMKAIQPHFGLAEE
                     ASNLGKLNSALGEKRAGRPAVAKPAATVTAAMKRLPPDAAITSLPKIGPKVAEKLEKL
                     EIRTIEDALRFAPRRYIDYSRTEKLGAALHQIGDVTVRGEIVEMRVHHTQAGKGRVTA
                     RLSDGTGTLRLTWFNTFIAKQLVEGDEIFVSGVIEGGFGGLQMTSPEWEHVESAGVST
                     GRMVPVYSSTQGLAQKTLRTMTRNALDATKTQLTDWLSEARPYMRPGAWERFPSLVDA
                     YEQLHYPESEEARDTAHTRLAFENLLLLQIGLVKRKGDQQAQKGEALTVHPEQLAAFR
                     DVLPFQLTGAQERSVGEILRDIQLPRPMTRLLQGDVGSGKTVVAAIAALVAKTSGAQT
                     AIMAPTEILAVQHLRNLTNLFAPMPDESRPSLALLTGSTRAAERRTIAAGLEAGEIDI
                     LIGTHALIQDHVSFASLGLVVIDEQHRFGVRQRGLLTEKAHGFQPHVLSMTATPIPRT
                     LNLVLNGDLDVSIIDERPPGRIPIETSRYVGRERADAYRIVREQITLGHQAFVICPLV
                     EESEAIEAKAAVEEAERLQREVFPDLRIAVLHGRMPSKQKDEVMAAFRNHEYDILVST
                     SVVEVGIDIPNATVMMIEGADRFGLSQLHQFRGRVGRGGARSFCLLLADDVSMTGNDR
                     LETMVATENGFELAEKDLELRGPGDFIGTRQSGLPELGWLDQGFDTRLLAVARETAER
                     IVEVSRAVDHDHFPLLGQKLRHFWASAAIVDAGKS"
     gene            complement(4754..5611)
                     /locus_tag="VFQ54_00240"
     CDS             complement(4754..5611)
                     /locus_tag="VFQ54_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU0163434.1"
                     /translation="MVDQERIAIVTDSASDLTLEAAALLGVTVVPLALRFGHDTFLDR
                     AEISTDALVARVEEAGILPATSSPSRVTFERVFRQLASTHDAIIAVLNSSRLSSTVKS
                     ATIAAANVRDQIPVEIVDSLNVSYGLGHQVLRAVDLVNSGVPADRAATQLRTEVPFNH
                     VVFFAEELDHLRRHGKVGKAPELLGNILQLKPILRIDEGQVIPFERVRTRAKALDLLV
                     GFTKSLPAIDAIAVIYDTLPDDLDPLVRRLRRLVPSREVPHIQIGPSLAAQVGPGVLG
                     IAIRERIVV"
BASE COUNT         1045 a         1869 c         2014 g         1106 t
ORIGIN      
        1 caaacgagag gcccgcgcga atcgattcgc acgggcctct cgtcatcacc acgatgccgg
       61 aaatggagcg ggcggcaggg ttcgaacctg cgacctatcg cttgggaagc aatcgctctt
      121 ccgtctgagc tacacccacc cgtgaaggac cgtttcctgt gttcctcctg tgattgaaaa
      181 cgccacggac atcggaaagg tgtcacattt cgttagccat tgtcaggtga gtcacagcaa
      241 gcgtacacgg tgaaggagtg cgccggcatc gcgccaagga tcagaatcgc gcgtcgcgac
      301 cgaccgcgtc ccgggtccgt tcgatcgtct ccagcgtctg ctcgaccgcg tcctccatca
      361 gcgccatgcc ccgccgaacg gagctctcga cgaggccgat ctgctccatg atgaagacat
      421 cgatctcgcc gcgggcgcgc tggcgttctt cctccgcctg gcgcagcaac tcctcgctgc
      481 gttgcctcgc ttcgatcgtg atgccgtgct gggagacgag acggtcggcg tcgacgcgcg
      541 catcctggat gatcttcagc gcgtcttcct gtgcctggcc gacaattcgc tcgcgctcct
      601 tgatcacacg tcgcgcctgc ttgatctcgt tcggcagggc ttcgcgaagc tggtcgacga
      661 gggtcaggaa ctcctcctcc tcgaccatca cgcgagtgct gaacggtacg cgcttcccac
      721 tgccgaccag ctcctcgagc tgatcgacga ggtacagaat gtctgcggcg ccatcctgcg
      781 acacctcgtg accaccgcgg tcattcattt gcttactccc gaacggttcg atcattggcc
      841 catgtgcgca tcggacggct cctgcgacac tcgatcattg tcgtattctc tcagttcctc
      901 atggcatttg acaaaatgtc gcgccagctt cgaggtcctt ggcatgccct accgcacgcc
      961 tggggccgag attgcgaatc gggccatcag tgcctcctcg acattcgcgg gaaccagccc
     1021 gtgaacgtta ccgccgagag acgagacttc ccggatgaac gtgctgctga tgaacgagtg
     1081 ctgcgcggac gtcatcaggc agacgacctc gatctccggt gcgagatgct cgttcatatg
     1141 ggcgtacttg aattcgtatt cgaaatccga gaccgcacga agacctcgca cgatcgtcga
     1201 ggcgcccttc tcgcgggcgt agttcacggt cagtccggtg aagctctcga cgacgatgcg
     1261 cgtggcgtag ggatcggtcg atgtccggat cgcctccttc gcgagcgcga tccgctcggc
     1321 aaaggagaag agcgccgtcg gcttctccga tccctcgtac accgagagga tgatgccgtc
     1381 gaagagacgg gccgcgcgca acgcgacatc gagatgcccg ttggtgatcg gatcgaaact
     1441 acctggatag atggcgagcg tcatcggtct tccccagttg ccggctccgt cccggcctgc
     1501 gatgcgtcat cgattgcaaa ctcatagatg gaaaaacagc tgtcgccgtg acaccggtgc
     1561 cggagccgtt cgaggttccc cacgcgatcg gggacctcga cgcgatcggc gtgccccaac
     1621 acgacgatcg ttcccggttc gaccagcggt gactccgcga tctgcccgag gatggggacg
     1681 atctgcggat ccgcgtaggg cggatcgacc atgatgaaat catacggcgt gcgggcggat
     1741 tgaatgaagg catcgacggg aatctggcgc acgtcggact tcgcggcgaa gtcggtgtgc
     1801 tcgagattcc agcggatcgt tgcgatctgc tccttcccct tgtcgacgaa ggtcacgaag
     1861 ctcgcgccgc gcgagagggt ctcgatgccg agcgccccgg tccccgcata gaggtcgagc
     1921 acgcggtcgg gttcgatgcc gagcgacatc agggaattga agagcgcgac cttcagcttg
     1981 tcggtcatgg gacgggtcgc gaaactcggt ggtcccttca actggcgacc gcgagcggaa
     2041 ccggatatca cacgcatcga ggtggcgaac tcctggcgga acagatatcc gcgtcaattg
     2101 gacgcggacg aagtcgaatg gagtatatcc ggaccggggg caggaatcgt tcaggacttc
     2161 ccggcatcga cgatcgcggc cgacgcccag aagtggcgga gcttctggcc gagcaggggg
     2221 aagtggtcgt gatcgacggc gcgcgagacc tcgacgatgc gctcggcggt ttcccgcgcg
     2281 accgccagga gtcgcgtatc gaatccctgg tcgagccagc cgagttcggg aagaccgctc
     2341 tgccgggtcc cgatgaaatc gccgggaccg cgcagctcga gatccttctc ggccagctcg
     2401 aagccgttct cggtagcgac catcgtttcg aggcggtcgt tgcccgtcat ggagacgtca
     2461 tcggcaagga ggaggcagaa gctccgggcg ccgccgcgcc caacccgacc gcggaactgg
     2521 tgaagctggg agagaccgaa gcggtcggcg ccctcgatca tcatcaccgt cgcgttcggg
     2581 atgtcgatgc cgacctcgac caccgaggtc gagacgagga tgtcgtactc gtgattgcgg
     2641 aaggcggcca tcacctcgtc cttctgcttc gacggcatcc ggccgtgaag gacggcgatg
     2701 cggagatcgg gaaagacctc gcgctggagg cgttccgctt cctcgacggc agccttcgcc
     2761 tcgatcgcct ccgattcctc gacgagcggg cagatcacga acgcctgatg accgagcgtg
     2821 atctgctcgc gcacgatccg gtacgcgtcc gctcgttcgc gtcctacata acggctggtt
     2881 tcgatcggaa tccggccggg cggtcgttcg tcgatgatcg agacgtcgag atcaccattg
     2941 aggacgagat tcagggtacg ggggatcgga gtcgccgtca tcgagaggac gtgcggctgg
     3001 aagccgtggg ctttctcggt gaggaggcca cgctggcgga caccgaaccg gtgctgctcg
     3061 tcgatgacga ccagcccgag cgaggcgaag ctgacatgat cctggatcag ggcgtgggtg
     3121 ccgatcagga tgtcgatctc gccggcttcg agcccggcag cgatcgtccg gcgttcggcg
     3181 gcgcgcgtcg agccggtcag cagcgcgagg ctgggacggg attcgtccgg catcggggcg
     3241 aagaggttgg tcagattgcg caggtgctgc acggcgagaa tttcggtcgg cgccatgatc
     3301 gcggtctggg cgccgctcgt cttcgcgacg agggcggcga tcgcggcgac gaccgtcttg
     3361 ccggacccga catcgccctg caggaggcgg gtcatcggtc gtgggagctg gatgtcgcgg
     3421 aggatctcgc cgaccgaacg ctcctgggcg ccggtcagct ggaatggcag cacgtcgcgg
     3481 aaggcggcaa gctgttccgg atgaaccgtg agcgcctctc ctttctgcgc ctgctgatcc
     3541 ccctttcgct tgaccagtcc gatctggagg aggaggagat tctcgaaggc aaggcgggta
     3601 tgtgccgtgt ctctggcttc ctcgctctcg gggtagtgga gctgctcgta ggcgtcgacg
     3661 agggacggaa agcgttccca cgcgccgggc cgcatgtagg gccgcgcctc gctcagccag
     3721 tctgtgagct gcgtcttcgt ggcgtccagc gcgttgcgcg tcatcgtgcg gagggtcttc
     3781 tgggcgagcc cctgtgtcga cgagtagacc gggaccatgc gtccggtcga gaccccggcg
     3841 ctctcgacat gctcccattc gggggacgtc atctggagac cgccgaaacc gccttcgatc
     3901 actccgctga cgaagatctc gtcgccttcg acgagctgct tggcgatgaa ggtgttgaac
     3961 caggtcagcc ggagcgtgcc cgttccgtcg ctcaggcgcg ccgtgacccg gcccttgccc
     4021 gcctgcgtgt gatggacgcg catctcgaca atctcgccgc gaacggtgac gtcgccgatc
     4081 tggtggaggg cggcgccgag tttctcggta cgggagtaat cgatgtaccg ccgcggcgcg
     4141 aatcgcagcg cgtcctcgat cgtccggatc tccagcttct cgagcttctc cgcgaccttc
     4201 ggtccgatct tcggcaggga ggtgatcgcg gcatcgggcg gaagccgctt catcgccgcc
     4261 gtgaccgtgg ctgccggttt ggcgacggcc gggcgtccgg cgcgcttctc gccgagcgcg
     4321 ctgttcagct tgccgagatt cgacgcttcc tcggcaagac cgaagtgggg ctggatcgcc
     4381 ttcatcaccg tggcgacctg ctggatctgc tcgcggcgga ccgactcgtt cgccgggagg
     4441 tgttcgagcg tcgcgatcgc cgtcacgatc gatcccccca cctcgtgggt gctcgccacg
     4501 cgtgcctgga gctcgcgaag catgcggacg gccaggagga tgtcccgctg cagtgccgga
     4561 ccgcccttcc aggcgcgacg aagcgcatcg agcatccgct cgaagcgttg ttcgttggtg
     4621 ttgcgtgaga catcagtgga gcggagggtc acgaagcagg tcctggatgg aatggagcag
     4681 atgctggcgt gcctgtcgaa tgcgaacgcc tgtacgcgtt cgcaccatgg taccgcaggt
     4741 cgtgggacgg agcttacacc acgatccgct cgcgaatggc gatcccgagc accccggggc
     4801 cgacctgtgc ggccagcgac gggccgatct ggatgtgggg gacttcccgg ctcggaacga
     4861 gtcgccggag tcgccgcacg agcggatcga gatcgtccgg cagtgtgtcg tagatcaccg
     4921 caatcgcgtc gatcgccggc agggatttcg tgaatcccac caggaggtcg agcgccttcg
     4981 cacgggtccg cacccgctcg aacgggatga cctggccttc gtcgatgcgg aggatcggtt
     5041 tcagctggag gatgttgcca agcagttccg gcgccttgcc aaccttgccg tgccgccgga
     5101 ggtgatcgag ctcctccgcg aagaagacca cgtgattgaa cgggacctcg gtgcgcagct
     5161 gtgtcgcggc acgatccgcc gggacgcccg aattgacgag atcgaccgcg cgcagaacct
     5221 gatgaccgag tccatagctg acgttgagcg aatcgacgat ctcgaccggg atctggtccc
     5281 ggacgttggc ggcggcgatc gtcgcggact tcaccgtgga actgagtcgt gacgaattca
     5341 gcacggcgat gatcgcatcg tgcgtcgacg cgagctggcg gaagacgcgc tcgaaggtga
     5401 cacgcgacgg agatgaggtc gcggggagga tgccggcctc ctcgacgcgg gcgacaaggg
     5461 cgtccgtgga gatctcggcg cgatcgagga acgtatcgtg accgaagcgc agcgccagcg
     5521 gcaccaccgt gacgccgaga agcgcggcgg cttccagcgt gaggtcggaa gcgctgtccg
     5581 tgacgattgc gatgcgttcc tgatctacca tgatatgggg ctctcctgtc ccgctgggat
     5641 ccgtcttctc catgattgag tcccgagatg tctggacggg cgcgaacaac cgggatgccc
     5701 gggcggcgaa agaagaacac cggcaaggct accgcagtgg aggacgcaac acacacctga
     5761 ttgtgacacc tcgggccggg cgatgctgcg caaggtgcat tcgtgtccct ttcttcacgc
     5821 aatgactcgc ggacggtcat gatcgggagt ggcgccacca gccggggatg tgctaaccta
     5881 gacgactgtg tatggcgtcg tccaatgacg acgccgattt cgcgcccggc tggctcgtcc
     5941 ggtcgagatg gcgaaagcta ctgagtatca tctgcataca ggcttgactg cgcgtgtgga
     6001 cgtcttcgtt tcccgagccg cggcaagaat gcaa
//
LOCUS       DASTMD010000017         1603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3036862, whole genome shotgun sequence.
ACCESSION   DASTMD010000017 DASTMD010000000
VERSION     DASTMD010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3036862"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VFQ54_00245"
     CDS             <1..743
                     /locus_tag="VFQ54_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0163435.1"
                     /translation="LIGVSTGASLGAVIAIVLNIGIWGIWTLPAFAFGGAMLVTLLVY
                     LIARHEGKTEVVTLLLAGVAINAMIGAGVGLLITRANDQEIRSVTFWTMGSLGGALWN
                     QSGIVFLATTIGVGVILRFAQALNLFVLGEREARHLGVRVERSRAIVMFTTAAMTGAS
                     VAFTGAIGFVGLVVPHLVRLWLGPDHRRLLPCAALLGATLMLFADLAARTIVQPMELP
                     IGVVTSLIGGPFFIWLLLRTRRQQGGWA"
     gene            764..1546
                     /locus_tag="VFQ54_00250"
     CDS             764..1546
                     /locus_tag="VFQ54_00250"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HEU0163436.1"
                     /translation="MIELRGVGLIRGERQLLDAIDLAIEPGEVIALVGPNGAGKSTLL
                     SVLAGDLAPSSGSMHLDGRRIQEWSIGDLARILAMLPQQTVLQFGFTGEEVVAMGRTP
                     LPVAERRRDAEAIGVAMRETESLHFASRRFPTLSGGEQTRVSLSRVLAQETRMLLLDE
                     PTAALDIRHQQLVMQRARTLADTGRTVIAILHDLNLAMSHADRLAILADGRLVAVERP
                     WGAAREGLLESVFQCPLIVARHPHLGCPLVLPMNADLASTQF"
BASE COUNT          224 a          503 c          567 g          309 t
ORIGIN      
        1 gtctgatcgg tgtctcgacc ggggcgtcgc tgggcgccgt gatcgcgatc gtgctcaaca
       61 tcgggatctg gggaatctgg acgctgccgg cattcgcctt tggcggggcg atgctcgtca
      121 cgctcctcgt ctacctgatc gcgcggcacg agggaaagac ggaggtggtg accctgcttc
      181 tcgcgggcgt cgcgatcaac gcgatgatcg gggccggggt cggtttgttg atcacgcgcg
      241 cgaacgatca ggagattcgc tcggtgacct tctggacgat gggcagcctg ggtggggcgc
      301 tctggaacca gagcggaatc gtcttcctgg cgacaacgat cggcgttggc gtcatcctgc
      361 ggttcgccca ggcattgaat ctcttcgtgc tcggtgagcg cgaggcgcgg catctcggcg
      421 tccgtgtcga acgaagccgg gcgatcgtga tgttcacgac ggcggcgatg accggcgcct
      481 cggtcgcctt cacgggggcg atcggtttcg tcgggctcgt ggtgccgcat ctcgtgagac
      541 tctggctcgg acccgatcac cggcggttgc tgccgtgtgc ggcgttgctt ggcgccacgt
      601 tgatgctctt cgccgatctc gccgctcgca cgatcgtgca gccgatggaa ctgccgatcg
      661 gcgtcgtcac gagtctgatc ggcggaccgt tcttcatctg gctgctgctc cggacgcggc
      721 gacagcaagg tggctgggcg tgaccggggg aacatcgtca cccttgatcg agcttcgggg
      781 cgtcggcctg atacgcggcg aacgccagtt gctggacgcg atcgatctgg ctatcgaacc
      841 tggcgaagtg atcgcgctcg ttggaccgaa cggcgcaggc aagtcaaccc tgctcagcgt
      901 gctcgccggg gatctggctc cgtcatccgg ctcgatgcat ctcgacggga ggcggatcca
      961 ggagtggtcg atcggtgatc ttgcccggat cctggcgatg ctgccgcaac agacggtgct
     1021 ccagttcgga ttcaccggcg aggaggttgt cgcgatgggg cggacgccgc tccccgtggc
     1081 ggagcgtcgc cgggatgccg aggcgatcgg cgtggcgatg cgcgagaccg aatcgcttca
     1141 tttcgcatcg cgccgctttc cgacactctc cgggggcgag cagacacgcg tctcgctttc
     1201 tcgcgtcctg gcgcaggaga cgcggatgct tctcctcgat gagccgaccg cggcgctcga
     1261 tatccggcac cagcaactcg tgatgcagcg tgcgcggacg ctcgccgaca ccggacgcac
     1321 cgtgatcgcg atcctccacg atctcaatct ggcgatgagc cacgccgatc gactggcgat
     1381 cctcgcggat ggacgtctcg tggccgtcga gcggccgtgg ggcgccgcgc gcgaggggct
     1441 gctggagagc gtgttccagt gcccgttgat cgtggcgcgt catccgcacc ttgggtgccc
     1501 gctcgtgctg ccgatgaacg ccgacctggc gtccacacaa ttctgagccg gcgggcatcg
     1561 ttcgcgcaga ccgttccggg tccgactcta cgtgtcgcgg cgg
//
LOCUS       DASTMD010000018         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1340398, whole genome shotgun sequence.
ACCESSION   DASTMD010000018 DASTMD010000000
VERSION     DASTMD010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340398"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFQ54_00255"
     CDS             complement(<1..497)
                     /locus_tag="VFQ54_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163437.1"
                     /translation="MPESHQLRFAYSTINWGSTCNLPSALEDIRAAGWQAIEFFGHSL
                     DLLGTPASLTQTMNGLIPATLFSSFDLPITDLQRTIHKNHIRFAAELGTGAYGLVGGS
                     RLRYRPPSVDDYAQLAAFCEELAVFGQDLDVVVSYHPHTLCTIETSAEIALLMAQTTA
                     LRLCL"
     gene            874..1644
                     /locus_tag="VFQ54_00260"
     CDS             874..1644
                     /locus_tag="VFQ54_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0163438.1"
                     /translation="MPVLDKFSLAGKVALVTGGNRGLGRAMAQAFGEAGARVAVSART
                     LSAAEAAAAELEGLGITAFGVQLEVTDPEDVERAVQAVEDGLGPVDVLLNNAGISAEH
                     TGLGDSNADFTAIMATNVDGVWHCSRAVARRMVARGAGGTIVNVGSMSAQIVNQPRWQ
                     PAYLASKAAVHQLTKGLAAEWAPLGIRVNAMAPGYFLTEMSPVDQPEFKPWCVDPAPM
                     KRHGYPEELGPVAVFLASDASSFMTGSIVNVDGGFTLF"
     gene            1708..>2649
                     /locus_tag="VFQ54_00265"
     CDS             1708..>2649
                     /locus_tag="VFQ54_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0163439.1"
                     /translation="MIRFGIVGAGLRGRMYQEALADVPDVEVVAFAEPSERVATLTRE
                     ATGLPVVTSAIELLSRHELDAVVVATPDFAHREAAVAVASAGKHLLLEKPLAMTLDDA
                     YAIRDAVVAGGAICLVGFENRWNPHVVSAKRIIDAGTVGTHITSSATLSNSWFVPTQM
                     LSWAAKSSPAWFLMPHTVDMLLWFSGRTPVSVSAVASSGILKAKGIDTQDVVHALIAF
                     DDGTTASLTSAWTLPDGTDAIVDFHFQFIGTDGSISGDPIHQGLDVATDRLRTQGTLS
                     GRIGTSQVGAPVWMAQEFAANLVQGKPTGPGVEQGVLV"
BASE COUNT          449 a          776 c          927 g          497 t
ORIGIN      
        1 tcgaggcaga gcctgagcgc ggtcgtctgc gccatcagca gcgcgatctc cgccgaggtt
       61 tcgatcgtgc agagcgtgtg cgggtgatag ctgacaacga catcgagatc ctgaccgaag
      121 acggcaagct cctcgcagaa cgccgcgagt tgcgcgtaat catcgacgga ggggggacgg
      181 tagcggaggc ggctgccgcc gacgagtccg tacgccccgg tgccgagttc cgccgcgaac
      241 cggatgtgat tcttgtggat cgtccgctgg agatcggtga tcgggagatc gaagctgctg
      301 aagagcgtcg ccgggatgag accgttcatg gtctgggtca ggctggctgg cgtgccgagc
      361 agatcgagcg agtgaccgaa gaactcgatc gcctgccagc ccgcggcgcg aatgtcttcc
      421 agtgccgagg gcagattgca ggtcgacccc cagttgatcg tgctgtaggc aaaacgaagc
      481 tgatgggatt cgggcatgaa ccgggttcgc ctttctgacc tttgccttcg acaagccacc
      541 actgcgccac gatggaacga atcgcgaaat cgggagacgc cggtcgcata ctgctgttgc
      601 gtctggcggc gtgtcctgtt cggaacccga ggatgtttgg tttggtcatt tttgccattg
      661 tagacgtcga ggcagggctg tcaatttggt tcagtacagg gaagacgacc ttgggcatca
      721 tgccgctgct tgttcgcttc agcgatccct gaacgtggga ggaacgcggc gtatcgtcag
      781 tgttggttgc caaggccggg gaaatccgct ctaatcccga gatgatcccg ggccaatgcg
      841 accgatggtg acgttcagac aggggagatt tcgatgccgg ttctcgacaa gttctcgttg
      901 gcaggcaagg tcgcgctcgt taccggtggt aaccggggat tgggccgggc gatggcccag
      961 gcattcggcg aggccggcgc cagggtcgcg gtctccgcgc ggacgctttc cgcggcggaa
     1021 gcggcagccg ccgagctgga ggggctgggc atcaccgctt tcggggtgca actcgaagtt
     1081 accgatccgg aggacgtcga gcgggccgtg caggcggtcg aggatggtct cggaccggtc
     1141 gacgtgctgc tcaacaacgc cgggatctcg gcggagcaca ccggtctcgg tgattccaac
     1201 gccgacttca cggcgatcat ggcgaccaac gtcgatggcg tctggcattg cagccgtgcc
     1261 gtcgcacggc ggatggttgc acgcggcgcg ggagggacga tcgtcaatgt cggctcgatg
     1321 tcggcgcaga tcgtgaatca gccgcgctgg cagccggctt acctcgcatc gaaggcggcg
     1381 gtccaccagt tgacgaaggg cctggcggcc gaatgggcgc cgctcggaat ccgcgtcaac
     1441 gcgatggctc ccggctactt cctgaccgag atgtcgccgg tcgaccagcc ggagttcaag
     1501 ccgtggtgcg tcgatccggc gccgatgaag cggcatggat atcccgagga gctgggaccg
     1561 gtcgcggtct tcctcgcgag tgacgcctcg agtttcatga ccggctcgat cgtcaatgtc
     1621 gacggcggat tcacgctctt ctaggctgac gccgccggtt tctctggtat cgtcaccgtc
     1681 catattcata aggaagaagc aaccagcatg atcagattcg gcatcgtggg cgccggcttg
     1741 cgcggacgca tgtatcagga agcactcgcc gacgtgccgg acgtcgaggt cgttgccttc
     1801 gccgaaccgt ccgaacgtgt cgcgacgctg acgcgggagg cgaccggatt gcctgtcgtg
     1861 acatcggcca tcgaattgct gtcgcggcac gagctcgatg ccgtcgtggt cgcgacgccg
     1921 gacttcgcgc atcgcgaggc ggcggtggcg gttgcttccg ccggcaagca tctcctgctc
     1981 gagaagccgc tggcgatgac gctggacgat gcctatgcca tccgtgatgc ggtggtcgcc
     2041 ggtggcgcga tatgcctggt cggtttcgag aatcgctgga atccgcacgt tgtttccgcg
     2101 aaacggatca tcgacgcggg aacggtcgga acacacatca cctcctcggc gacgctctcg
     2161 aattcctggt tcgtgccgac gcagatgctc tcctgggcgg cgaagtcgtc cccggcctgg
     2221 ttcctgatgc cgcacacggt ggatatgctc ctctggttct cggggcggac gccggtctcc
     2281 gtctcggcgg tggcgtcgag cgggatcctg aaggcgaagg ggatcgacac gcaggatgtc
     2341 gtgcatgcgc tgatcgcgtt cgacgacggg acgacggcgt ccctgacctc ggcgtggacg
     2401 ctgccggatg gcaccgacgc gatcgtcgac ttccacttcc agttcatcgg caccgatggc
     2461 tcgatctcgg gcgatccgat tcaccagggg ctcgacgtcg cgacggatcg cctgcgaacg
     2521 cagggcacgt tgagtggacg cattgggaca tcgcaagtgg gagcaccggt ctggatggcg
     2581 caggagttcg cggcgaacct ggttcagggc aagccgaccg gaccgggcgt ggagcagggc
     2641 gtcctggtg
//
LOCUS       DASTMD010000019         1780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_269560, whole genome shotgun sequence.
ACCESSION   DASTMD010000019 DASTMD010000000
VERSION     DASTMD010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269560"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFQ54_00270"
     CDS             complement(<1..368)
                     /locus_tag="VFQ54_00270"
                     /inference="COORDINATES: protein motif:HMM:NF019872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU0163440.1"
                     /translation="MAVATVPGYPRIGKNRELKKALEAFWSNKITDDDLLRTAYEIQH
                     TNWEAQAAAGLDLLPVNDFSFYDQMLDTLGLVGAVPARYEWDGGAPGMDTYFAMARGR
                     TGERDVPAMEMTKWFDTNYH"
     gene            748..1200
                     /locus_tag="VFQ54_00275"
     CDS             748..1200
                     /locus_tag="VFQ54_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163441.1"
                     /translation="MADDLKQVLNYIVNRFVVDDELRTIDREAPVMAIAPTEPDRDEV
                     AQREESRAVSLADAFVRGLIRAWQRFTMGGNELPLDDRKEDENRMADALIRFLVSYDL
                     ASSRTEVTGDRTYTYYLSIRWPQLRDVALAAGVDLDRALSRQLDRSSR"
     gene            1341..>1780
                     /locus_tag="VFQ54_00280"
     CDS             1341..>1780
                     /locus_tag="VFQ54_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HEU0163442.1"
                     /translation="MSSTSDLASTVSAPRAVSGPTPYRMPDAEADLAFARNTGIEFRD
                     IRTLRLALTHRSVLHDWINVEHVDAILQSNERLEFLGDALLGMVVGEFLYRTDPTADE
                     GALTRHRAATVRAETLVRWARELKLQDYLYLGTGESVTHSARDR"
BASE COUNT          337 a          574 c          537 g          332 t
ORIGIN      
        1 tagtggtagt tggtgtcgaa ccacttcgtc atttccatcg cggggacgtc gcgttcaccg
       61 gtgcgaccgc gtgccattgc gaagtaggtg tccatgccgg gcgcaccgcc atcccactcg
      121 tagcgagcgg ggacggcccc gacgagaccg agggtatcga gcatctggtc gtagaacgaa
      181 aagtcgttga cgggaagcag gtcgagcccg gcggcagcct gagcctccca gttggtgtgc
      241 tgaatctcgt aggcggtccg gagaagatca tcgtcggtga tcttgttcga ccagaacgct
      301 tcgagggcct tcttcaactc gcggtttttg ccaatccgcg ggtatcccgg aaccgtggcg
      361 actgccatgc agaactccct gctactgacc tggtagcgga actccgaatc cctatgaccg
      421 gagcatgaaa ccaggtggat cgaatccgag atgagcctat cacgcggatg ccgcgtccgg
      481 agcgagcagc gtgggcggag tcccctccga ccgcgcaggt cacatctcac ggtagctgca
      541 catcgtcgat atgatgcgta actatcaaaa gtatagcgaa gtggtcaagc atagattccg
      601 ggagggaagg cgcgctccgg cgagaacgcg gccgtcgaca ctcaatgcct gagattctct
      661 ggcgctcgcc cgggactggg tatatgtttg agccatgcgc ggatcgtttg cggcttggcg
      721 ggcgatctcc actcgacgag ggatgacgtg gccgacgatc tgaagcaggt gctgaattac
      781 atcgtgaacc gcttcgtcgt ggatgacgag cttcgcacga tcgatcgcga ggcgccggtc
      841 atggcgatcg cgccaacgga acccgatcgc gacgaggtgg cgcagcgcga ggaatcgcgc
      901 gcggtctcgc tggccgacgc cttcgtgcgt ggactgatcc gcgcctggca gcgcttcacg
      961 atgggcggga acgaactccc actcgacgat cgcaaggagg atgagaatcg gatggcggat
     1021 gccctcatcc gttttctcgt cagctatgac cttgccagct cacgcaccga ggttaccggc
     1081 gaccgcacct acacctacta tctctcgatc cgatggccgc aactgcgtga tgtcgccctc
     1141 gccgccggtg tcgatctcga cagggcgctc tcccggcaac tcgatcgatc gtcgcgatag
     1201 caacccccaa ccggtcatct ccgccgcaca ccacggttac ctttgtggca ccatatccgc
     1261 gacgctccgg acgcgttctt ccggtcgccg cactagtcac aaccaccgtt cgctccgcat
     1321 acccacatga aatgaacgaa atgagttcaa cgtccgacct cgcatcgact gtttccgcac
     1381 cgcgcgccgt atccgggccg accccatacc ggatgccgga tgccgaggct gatctcgcgt
     1441 ttgcccgcaa cacggggatc gagtttcgcg atatccgaac ccttcgcctc gcgcttacgc
     1501 atcgctccgt gctccatgac tggatcaacg tcgagcacgt cgacgccatc ctccagagca
     1561 acgagcgact cgaattcctc ggcgacgcgc tgctcgggat ggtggtcggc gagtttctct
     1621 accgtaccga tccgacagcc gacgagggcg cgctcacgcg ccaccgcgcc gccaccgtgc
     1681 gtgcggaaac gctcgtccgc tgggcgcgcg aactgaagct tcaggattac ctctacctcg
     1741 ggaccgggga atcggtcacg cacagtgccc gggatcgcat
//
LOCUS       DASTMD010000020         3261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2769156, whole genome shotgun sequence.
ACCESSION   DASTMD010000020 DASTMD010000000
VERSION     DASTMD010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2769156"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            577..969
                     /locus_tag="VFQ54_00285"
     CDS             577..969
                     /locus_tag="VFQ54_00285"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEU0163443.1"
                     /translation="MSAWFTRFSRKASEWTGHYVAFLLAALFIVVWAATGPLFHYGQN
                     WQLIVNTSTTIITFLMVFLVQHTQNHDSKALHIKLDAIIEALERADNRIMGAEEDTEE
                     ELASLKAKYETPTDVTSSNGVAASVPGR"
     gene            1449..>3261
                     /locus_tag="VFQ54_00290"
     CDS             1449..>3261
                     /locus_tag="VFQ54_00290"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163444.1"
                     /translation="METTVRGSRARSAPAAQSPHATVIRPRLFERIGQPDAAITLITA
                     PAGYGKSTLLAQWRDSAATPVAWIRIGIASNSLVSLLDTIGMEVANALYRDGDPPVRT
                     VDNLLDWLHALSDDGERLTLIFDDYHLIANQAVHEALDTLIALRPSGISLIVSSRTEV
                     PLALGRLRAAGQVRQLTAADLAFTADETTSVAGQAQIPLTHRQIVTLGERTEGWIAGI
                     RLALLSLEHVATDAVESVVDTWSATRWLDDYVVEEVLATLPDDVRDFVERTTMLAELT
                     PELCDAVLESTRSADLLNVVATRLVFVRATGAPGGALAYHPLFAESVERIVSRRRTSE
                     ELSEAHRRAAVWFAGQGSHEAAIEHGVRAEAWDLVEDEVRIVFSPLADGGQHYSLLYW
                     LGKLPTSRMLADSLMAFRFVQSLQFTGQIREARRWFDLVEPTWMASGDPWQLGHAAEC
                     RTFFFSLDGNPDASLRNAYVALGHTPIERTLERMYIWGSIIQTEFFKGNEVVVAEAYR
                     QADRCHDVLPNQSWLWLLIEPDRANHHFLRGRLRDGAELCRHVIAHVPTSYATRIGRV
                     RFRLAAVELEWNHLDAAMDIADLVEEDMAWFPVQLWYP"
BASE COUNT          564 a         1032 c         1042 g          623 t
ORIGIN      
        1 tgctgaaggc attcgtcgcc cacggggagg cgggacattc ctccatccac agaccgggcg
       61 ccttgcccca aagacaccct gaatgccggt ctttggattc cgcagtcccg ccctacgtac
      121 gaccgcggta tccagcacag aggtcgaggt aggagtcgtg cgggaggagc tccgcactcc
      181 gagacgcggc tcgggacatt cgatcgtagg tgtagggtct cacagcgaat accgggacga
      241 ttgccacaca tgctgctgga tcggacgcgg cgttccgcgt ggacgtcggt cgttcgtagg
      301 gcgggaccgc cgctgccgga ccgcagggtc ggacggcctg tcccgcctcg cgctcgcccg
      361 aggatcggcg acgccgcatt cacccggggt ggctgctcct gcgggtttgg ttcttctcgg
      421 ttcttctggt tcttctatgg aaaggagtcc ggacgcgtgc gacgtccgga ctcctttcca
      481 tttcgcacga cggaagaacg aattggcatc caacgtgctt ataggtgctc tggtcgagcg
      541 ggaccgccca gtgttgtcga gatcaggagg caaggcatgt cggcgtggtt tacccggttt
      601 tcccggaagg catcggagtg gaccggccat tacgtcgcgt tcctgctggc agccttgttc
      661 atcgtagtct gggccgcgac cggtccgctc tttcactatg gccagaactg gcaactcatc
      721 gtcaatacgt cgacgacgat cattaccttc ctcatggtct ttctggtgca acatacccag
      781 aatcacgact cgaaggcgct gcatatcaag ctcgacgcga tcatcgaggc gctcgagcgc
      841 gcggacaacc ggatcatggg cgccgaggaa gacaccgagg aagaacttgc cagcctcaag
      901 gcgaagtacg aaacgcccac cgacgtgacg tcctccaatg gcgtggcggc atccgtccct
      961 ggccggtgag cggcgtgttt ctgcgttcag gacgaagccc gggtggcgat cgccacccgg
     1021 gcttcgtcct tgcaacggcg cgatacgagc tgcggatcac gtagaatcca gcccgcgaaa
     1081 atacgtaaga atacggatac cgtgggcacg ccacatgtgc agccgctgac gacggtcatc
     1141 atggacgatc ggccaggcat ggcccggatt cgtgaggatg gctcgggccg tgctcgctcc
     1201 ggtgatgaca atccattcca ggttgccggg ggcgtcgatt ccgacatgtc gctatatacg
     1261 gcaccattgt gcatatgtcg ctgatttcgt gctgtgcgca ccctatatgt ctagtccaat
     1321 catgcgagaa tgggccaacg tggtggctgg ctgtcgccca cggacaactt caaacaaaaa
     1381 cctgcgaagg cgagcgcgag cttgcccgca ttgatgtgcg ccccgcggag gggaaggaca
     1441 agtcgaccgt ggagacgaca gtacgagggt cccgggcgcg atcggcgcct gcggcccaat
     1501 caccccatgc aacggttatt cgcccgcgac ttttcgagcg gatcgggcaa ccggacgccg
     1561 ccatcaccct gatcacggca ccagccggct atggcaaatc gaccctgctc gcccagtggc
     1621 gcgattccgc cgcgacgccg gtcgcgtgga tccgaatcgg catcgcgtcg aattccctcg
     1681 tctcgctcct cgatacgatc gggatggaag tcgccaacgc cctctatcgc gacggcgatc
     1741 ctccggttcg cacggtcgac aacctgctcg actggctgca cgcactgtcc gatgatggcg
     1801 agcgcctgac gctgatcttc gatgactacc atctgatcgc gaatcaggcc gtgcacgagg
     1861 cgcttgatac cctgatcgca cttcgcccgt ccgggatctc gctcatcgtg tcgagccgga
     1921 ccgaagtgcc cctggcgctc ggtcgcttgc gcgcggcggg gcaggtgcgc cagctgacgg
     1981 ccgccgacct cgcgttcacc gcggacgaga cgacgtcggt cgccggccag gcgcagatcc
     2041 cgctcacgca tcgccagatc gtgacactgg gtgagcgaac cgaaggctgg atcgccggga
     2101 tccgcctggc gctcctctcg ctcgaacacg tcgcgacgga tgccgtggaa tcggtcgtcg
     2161 atacctggtc cgccacgcgc tggctggatg actacgtcgt cgaggaggtg ctggcgacgc
     2221 ttcccgacga cgtccgcgat ttcgtcgagc ggacgacgat gctcgccgaa ctgacgccgg
     2281 agttgtgcga cgcggtcctg gaaagcacgc ggagcgcgga tctgctgaac gtggtggcga
     2341 ccagactggt cttcgttcgc gccaccgggg ctcccggggg tgcgctcgcc tatcatccgc
     2401 tcttcgcgga gagcgtcgag cggatcgtct cgcggcggag aacgtccgag gaactctcgg
     2461 aagcgcaccg gcgtgccgcc gtgtggttcg ccggacaggg gagccatgaa gccgcgatcg
     2521 agcacggcgt ccgggccgag gcgtgggatc tggtcgagga tgaggttcgc atcgtcttca
     2581 gtccccttgc cgatggcggt cagcattatt ccctgctcta ctggttgggc aagctcccga
     2641 cgagtcgcat gcttgccgat tcgctgatgg cattccgctt cgtgcagtcc ctgcaattca
     2701 ccgggcagat ccgggaggcc cgccgctggt tcgatctcgt cgagccaacg tggatggcct
     2761 caggcgatcc ctggcagctc ggacacgcgg ccgagtgccg cacgttcttc ttctcgttgg
     2821 atggaaaccc tgacgcctcc ttgcggaacg cctacgtcgc tctcggtcac acgcccatcg
     2881 aacgcacgct cgagcggatg tacatctggg gttcgatcat ccagaccgaa ttcttcaagg
     2941 ggaacgaagt ggtcgtggcg gaggcctacc ggcaggccga tcgatgccac gatgtccttc
     3001 ccaaccagtc ctggttgtgg ctcctgatcg agccggatcg tgcgaatcat cacttcctgc
     3061 gcggtcgact tcgtgatggc gccgaacttt gtcgccacgt gatcgcgcac gttcccacga
     3121 gttatgcgac gcgaatcggg agagtccggt ttcggctggc cgcggtcgaa ctcgagtgga
     3181 atcacctgga tgccgccatg gatatcgccg atctcgtcga ggaggacatg gcctggtttc
     3241 ccgtgcagtt gtggtacccg g
//
LOCUS       DASTMD010000021         4110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1519082, whole genome shotgun sequence.
ACCESSION   DASTMD010000021 DASTMD010000000
VERSION     DASTMD010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519082"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFQ54_00295"
     CDS             <1..597
                     /locus_tag="VFQ54_00295"
                     /inference="COORDINATES: protein motif:HMM:NF028352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HEU0163445.1"
                     /translation="RVLAGSAGLARQLALVLAPGADRVLAHQPASGPILVVAGSRHDT
                     TARQVEAMRQAAYPVVALAQDHLDDPMFPIDDVIERVSGYLASGRSAILTTSGLEPSS
                     SHPSFVVARLAEIASAGSVRELLAGLVLTGGDVAAGILSRLGATSFRLGGEIRPAMPW
                     GILDIGNGRSLPVVTKAGSFGEDDALLAAVRSLEARQH"
     gene            complement(623..1666)
                     /locus_tag="VFQ54_00300"
     CDS             complement(623..1666)
                     /locus_tag="VFQ54_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEU0163446.1"
                     /translation="MTTYRAMIASPLEREHADRIQKELPAGIELIYEPALFPPTRYVA
                     DHQGLPDFRRSPEDQARWDALIASADILFDFPFTNRHPHSYAPNLKWVQTTSAGVGQA
                     ASRMGIKPGELMITTASGVHARPLAEFVFLVLLTAVKDQVRLTADQRAHRWERICEDE
                     LSGKTLAIIGPGKIGQEVARIGRCFDMKPIALARDSRPERAAELGVDAIYTRDRLIEM
                     VSIADALVICAPHTPETEGMLDRTAFDALKPGVILVNIGRGQLVDEGAMLDKLRDGTI
                     RLAGLDVFQTEPLPDDSPLWDLPNVMVSPHSASTTALENARIVDIFIHNLSCFAEGRL
                     SEMRNVLDIDRMY"
     gene            2299..2736
                     /locus_tag="VFQ54_00305"
     CDS             2299..2736
                     /locus_tag="VFQ54_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163447.1"
                     /translation="MNVGGPNILITTQRQQVPFFLRFLWFIVVGWWLSGLFIFLGYFF
                     SFTVIGLPVGFWFLNRVPQAQTLRMRNTEFQMHQKDGYMHFKEGRTVQLPWYYRAIYF
                     PFGLVLGAVWLGAAWVISLPIITLPLSVWMIDRAPTIISLQRN"
     gene            2943..4010
                     /locus_tag="VFQ54_00310"
     CDS             2943..4010
                     /locus_tag="VFQ54_00310"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0163448.1"
                     /translation="MSATVLDTLIELTSLPGPTGQEELVLAWCRDRWSQLGAEVEVQP
                     VGNVLARIPGNGPKLMIQGHADEIGFMVKSIDDRGFVWLATAQVGPRIPHDRYPVGQP
                     ALVLGRDGAQVPGLFATVTGHILVTRAEKHTLEANDLFVDLGVETKEEAEALGVYVGA
                     NVIWNPPVRRIGKRYVSKAIDDRIALALVTHLIADIDRTALAYDLTIAATVQEEIGLI
                     GATSLTRGGYDLAIAIDNGPLGDYPGVDPREMPIKLGHGPTLVYKDGSAHYDRRIIKR
                     LLDVATVNQIPVQESVVQGMGTDGAAMIRSGVPTAMMMMATRYTHSPLEMGDERDLEA
                     ALALLKAFVTTPASPLPLGPS"
BASE COUNT          688 a         1334 c         1301 g          787 t
ORIGIN      
        1 cgcgtccttg ccggatcggc cggactggcg cgacaactgg ccctggtcct cgcgccaggc
       61 gccgatcgag ttctcgctca ccagcccgcg tccggcccga tccttgttgt cgcgggaagc
      121 cggcatgaca cgacggcgag acaggtggag gcgatgcgcc aggcggcata tcctgtcgtc
      181 gctctcgctc aggatcatct cgacgacccg atgtttccga ttgacgacgt catcgaacgc
      241 gtctcgggtt atctcgcctc cggtcgatcc gcgatcctga cgaccagcgg gctcgagccc
      301 tcatccagtc atccgtcgtt cgtcgtcgcg cggctggccg aaatcgcctc cgccggtagc
      361 gtgcgggaac tgctcgcagg actcgtgctc accggaggag atgtcgccgc ggggatcctc
      421 tcacgactcg gcgcgacatc gtttcgcctt ggcggcgaga tccgaccagc gatgccgtgg
      481 ggaatcctcg atatcgggaa cggtcgctcg ctgcccgtcg tgaccaaggc cgggagcttc
      541 ggcgaggacg acgccctcct cgccgccgtg cgctccctgg aggcgcggca gcactgagca
      601 agcccggacg gcgccgtccc cctcagtaca tgcggtcgat gtcgagaacg ttgcgcatct
      661 cgctcagccg tccctcggcg aagcagctga ggttgtggat gaagatgtcg acgatccggg
      721 cattttccag cgccgttgtg ctcgcggagt gcggactgac catcacgttc gggagatccc
      781 acagcggaga atcgtctggc aggggttccg tctggaagac atcgagaccc gcaagccgga
      841 tcgtgccgtc gcgaagcttg tccagcatcg cgccttcgtc gaccaactgc ccgcgaccga
      901 tgttcaccag gatcacgccc ggcttgaggg catcgaatgc agttcggtcc agcatgcctt
      961 ccgtctcagg tgtatgcggc gcgcagatga ccagggcgtc ggcgatggag accatctcga
     1021 tcaaccggtc gcgggtgtag atcgcatcca cgccaagttc ggccgcgcgt tcagggcgac
     1081 tgtcgcgggc gagcgcgatc ggcttcatgt cgaagcagcg cccgattctg gcgacctcct
     1141 gcccgatctt gccgggaccg atgatggcga gcgtcttgcc actcagttcg tcctcacaga
     1201 tccgctccca ccgatgcgcc cgttggtcgg cggtcaggcg aacctggtcc ttgactgccg
     1261 tgagcaggac gaggaagacg aactccgcga gcggccgcgc atgaacgccg cttgccgtcg
     1321 tgatcatcag ctcgccgggt ttgattccca tccgcgatgc cgcctgccct actcccgcgc
     1381 tcgtcgtctg cacccacttc aggttcggcg cgtaggaatg gggatgccga ttcgtgaacg
     1441 ggaagtcgaa gaggatgtcc gcgctcgcga tcagcgcgtc ccatcttgcc tggtcttcgg
     1501 gcgaccggcg gaaatccggc aggccctgat gatcggcgac atatcgggtc ggcggaaaca
     1561 gtgccggctc gtagatcagc tcgattccgg ccggaagctc tttctggatc cgatcggcgt
     1621 gttctcgttc gagcggtgac gcaatcattg cgcggtatgt tgtcacgtag gggtccccgt
     1681 tcattggtga agcagacagc aatgattgat cgccgtcgtt atgtcgcgcc cttcgagcga
     1741 atcgcgatcg tcgtctggcg cggatcgtac caacgaaacc tgatcgcgcg aagacccccg
     1801 tttcgcccga tcaccaggtg gaatcggcat gacgttcccg gtcgcggacg attgctcccc
     1861 tccggacgcc ccggaatatc agtaagaata cggatatcca ttttcggaac cacgctgtaa
     1921 gattcccttg ggactaggaa atctgtgtca cgcgagcgcg gagacgtccg cacgcgatcc
     1981 atctccatat ctcctgcatt cctgcgacag gttgtcgcgg gttctcttcc tgcgttggtg
     2041 gggtgtttca ctatgtcaga aaatggaaag tcgggcccgt cgggcggtgt tcatccgggc
     2101 tccccgccag cgggggctgg atctcaaagc ggccacgttc cggcgccgac cggcccgaca
     2161 gccgccgtca cgcagccatg gaccttttcg tccgatccac cgcctccgcc gctaccgccg
     2221 gcggatgtcg cctatccgtc cgccggaggg tatgggcaat cagccgcgaa ctccaatatc
     2281 accaacgtgg cgacgaccgt gaatgtcggt ggtccgaata tcctcatcac cacgcagcgt
     2341 cagcaagtgc cgttctttct tcgcttcctg tggttcatcg ttgtcgggtg gtggctctcg
     2401 ggactcttca tcttcctggg ctatttcttt tccttcacgg tcatcggcct tcccgtgggc
     2461 ttctggtttc tcaatcgggt gccccaggcc cagacacttc gcatgcgcaa caccgaattc
     2521 cagatgcacc agaaagatgg atacatgcat ttcaaagagg ggcggacggt gcagctgccc
     2581 tggtactacc gggcaatcta ctttcctttc gggctggtgc tcggcgccgt ttggctcggc
     2641 gccgcgtggg tgatcagcct cccgatcatc accctgccgc tttcggtctg gatgatcgac
     2701 cgtgctccaa cgatcatcag cctccagcga aactaggatc cttccgggtg acgaattcgg
     2761 tctcctccgc tggcgaagca cgctccccga ttccggggag cgtgcttcgc gtggccgcgg
     2821 gatcggcgtg accgaagcga ggtgaggtgc atgtccgtcg gagcgttccc gtagcgcgat
     2881 cgccggagtg gccggataat cgccctcatg tcgttcgcgc agtcatggga gtccgatccg
     2941 aaatgtcagc aactgttctc gataccttga ttgaattgac gagcctgccc ggtccgaccg
     3001 gccaggagga actggtgctg gcctggtgcc gggatcgctg gagccagctt ggcgccgagg
     3061 tcgaggtcca gcccgtgggc aatgtgctcg cgcgtatccc cggcaacggc ccgaagctga
     3121 tgatccaggg acacgccgac gagatcggct tcatggtcaa gtcgatcgat gaccgcggct
     3181 ttgtctggct cgcaactgcc caggtcggac cgcgcatccc gcacgatcgt taccccgttg
     3241 gccagccggc actcgtgctc ggacgggacg gggcgcaggt cccggggctc ttcgcgacgg
     3301 taaccggtca catcctcgtg acgcgggccg agaagcatac gctcgaggcc aacgatctct
     3361 ttgtcgatct cggtgtggaa acgaaagagg aagcggaggc gctcggcgtg tacgtcggcg
     3421 cgaacgtgat ctggaacccg cccgtccgcc ggatcggcaa gcgctacgtg agcaaggcga
     3481 tcgatgatcg catcgcgctg gcgcttgtca ctcacctgat cgcggacatc gatcgcacgg
     3541 cgctcgccta cgacctcacg atcgccgcta ccgtccagga ggagatcgga ttgatcggcg
     3601 ccacctcgct gacgcggggt ggatacgatc tcgcgatcgc gatcgacaac ggaccgctcg
     3661 gcgactaccc cggcgtcgac ccgcgcgaga tgccgatcaa gctcggccac ggtccgaccc
     3721 tcgtctacaa ggatggctcg gcccattacg accggcgcat catcaagcgg ctgctcgatg
     3781 tcgcgaccgt gaaccagatc ccggttcagg agagtgtggt gcaggggatg ggaaccgatg
     3841 gtgccgcgat gattcggagc ggtgtcccca cggcgatgat gatgatggcc acgcggtaca
     3901 cgcactcccc gctggagatg ggcgacgagc gcgacctcga ggcggcgctg gcgctgctca
     3961 aggccttcgt cacaacgcca gcatcaccgc tgccgctcgg tccgtcgtag gggaccaccc
     4021 cggatcccgg cgaggaccgt gtccgcgatt gaagccgaag gagccccggc ggcggtcgcc
     4081 gtttcatcag gaacggcgac cgccgcgtgc
//
LOCUS       DASTMD010000022         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2679977, whole genome shotgun sequence.
ACCESSION   DASTMD010000022 DASTMD010000000
VERSION     DASTMD010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2679977"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="VFQ54_00315"
     CDS             complement(<1..1661)
                     /locus_tag="VFQ54_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0163449.1"
                     /translation="MSLGENESDSLITVAGKVLNERQLNRRKMMKGLLAGGAFATLAG
                     MPIRAGAQTPEASPVASPAASPVPYTGEVASDQEMRLPLSEPVTMDPGVSYGDSELEV
                     FANIFDGLVGIDPETGKIVNRIAESYTANADFSQFTFKIRQDVTWSDGTPLNANDFEY
                     SWKRVLDPNTISQYTSAMEYLKNGADIEAGKADLDTLGVKATDDYTLVVDCAGPSPFF
                     PLVAATWTYVAVPKHVIDAQGDAWVEAKNIVSSGPFKMTDWTHNQQIVMEPNDKYWGD
                     QPKLTKATYRIFKDPTTQAYVAFENNELDYAEPEGPDLDRVLADPKASANLLQFPASN
                     CYFIVCDTTNTPTDKPEFRQALYKSIDRNLIATQILKNQFLPAFTVMSPDIPGNNPAA
                     ALTESVDEAKALLQTAGIDPGSVNLEIVYVSDTARYKTIAEYLQSAWQTGLGITVKLS
                     PIENAAYSDWRASRETQSFSTYTGTWGSDYADASNWLNQNFTSSSDHYRDHWKDDDFD
                     ALCAKAVINKNTEERDQQYAAAEKILVDQAAIIPMYRGKNFRALKP"
BASE COUNT          422 a          676 c          721 g          489 t
ORIGIN      
        1 ggcttgagtg cccggaagtt cttgccgcgg tacatcggaa tgatcgccgc ctgatcgacg
       61 aggatcttct cggcagcggc gtactgctga tcgcgctctt cggtgttctt gttgatcacg
      121 gccttggcgc agagggcgtc gaagtcatca tccttccagt gatcgcggta gtgatcgctg
      181 gagctggtga agttctggtt gagccagtta ctggcgtccg catagtcgga gccccaggtg
      241 ccggtatacg tgctgaaaga ttgagtctca cgagaggcac gccagtcgct gtacgcggcg
      301 ttctcgatcg gagagagctt gaccgtgatt cctaggccag tctgccaggc cgactgcagg
      361 tactcggcaa tcgtcttgta gcgggcggtg tcgctgacat agacgatctc aagattgacc
      421 gaaccgggat cgatgccggc ggtctgcagc agcgccttcg cctcatcgac actctcggtc
      481 aacgccgccg ccgggttgtt gcccgggata tccggagaca tcaccgtaaa cgccgggaga
      541 aactggttct tgaggatctg ggtcgcgatc aggttgcgat cgatcgactt gtagagcgcc
      601 tggcggaact ccggcttgtc ggtcggggta tttgttgtgt cgcagacaat gaagtagcag
      661 ttggacgccg ggaactggag cagattcgcc gacgccttcg gatcggcgag tacgcggtcg
      721 aggtccggcc cttccggctc ggcgtagtcg agttcgttgt tctcgaacgc aacataggcc
      781 tgcgtcgtcg ggtccttgaa gatgcgatag gtcgccttgg tcagcttcgg ctggtcgccc
      841 caatacttgt cattgggttc catcacgatc tgctggttgt gcgtccagtc cgtcatcttg
      901 aatggaccgc tcgagacgat gttcttcgcc tcgacccagg catcgccctg cgcgtcgatc
      961 acatgcttcg gcactgcgac gtacgtccac gtcgccgcaa cgagcgggaa gaacggtgat
     1021 ggtccggcgc aatcgacaac gagggtgtag tcgtcggttg ccttcacacc gagggtatcg
     1081 agatcggcct tgccggcctc gatgtcagcg ccattcttca ggtattccat tgctgaggtg
     1141 tattgggaga tcgtgttcgg atcgagaacg cgcttccagc tgtactcgaa gtcgttggcg
     1201 ttgagcggtg tcccatcaga ccaggtcaca tcctggcgga tcttgaatgt gaactggctg
     1261 aaatcagcgt tcgccgtgta gctctccgcg atgcgattga cgatcttgcc cgtctccgga
     1321 tcgatgccaa ccaggccatc gaagatgttg gcgaaaactt cgagctcgct gtcgccgtag
     1381 ctgacgcccg ggtccatcgt caccggctcg gagagcggga gacgcatttc ctgatcggaa
     1441 gcgacctcac ccgtgtacgg taccgggctc gccgcgggac tggcgaccgg gctcgcctcc
     1501 ggcgtctggg cgcccgcccg gatcggcatt ccggcgaggg tagcgaaggc gccgccggca
     1561 agcagcccct tcatcatctt gcgacgattc agctggcgtt cgttgagaac cttgccagcg
     1621 acggtgatca aggaatcaga ctcgttctca cccaggctca tgatgctccc ttttcagatc
     1681 cgcggcgcca agggcaccgt caccaccaga tagcgaaccg gccaaccggc cggggacgtt
     1741 caccacaact ctcgctcgtt tccgccgggc gcaacgaaga tggctgccat tcccggggat
     1801 tgaccgattc gattgaaccc cctgatgctc gtggccccga tattcgatgg cagcgtcgcc
     1861 attcctgtcg tgaattgtgc agacgttagc agtgcgaatg ggttaccgtc aatagaatca
     1921 tgcagttcgc acggttttgc catgccggaa tgggactaat ggggcatagg tttttgggcg
     1981 catcgcacat aattcaatta gttgctttcc ctctgcacac cgagtccgac gaacgcaaac
     2041 acccgtgcac accgggagcg atccgacggc gcggtcgatc ccggtgggag gcagggcata
     2101 cctccatcca gaccggacat gatcttccgg aagcaacgac cgggccaggc tgggctccgt
     2161 agtcctgccc acgcaggaga cggagcacgg aagatcttgc ctctcaggga cgcggcgatc
     2221 gacgagccgc tggaacggcg ttcgtgcgac gttcatatcg agctgcgctc gatgggtcac
     2281 acggggtgac ccactacgac gggaatcg
//
LOCUS       DASTMD010000023         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2769242, whole genome shotgun sequence.
ACCESSION   DASTMD010000023 DASTMD010000000
VERSION     DASTMD010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2769242"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..636)
                     /locus_tag="VFQ54_00320"
     CDS             complement(244..636)
                     /locus_tag="VFQ54_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163450.1"
                     /translation="MTPDNDSPDETATPNGQEHSRNRNIETLRYEAAVVPRIAELLDV
                     KVQRAPFRLPSSTVWQLTVPGTQGRPVAMITLWPELNRVDVIAGSAAIVFTDVRTVDL
                     VPGVEVQFRRGSREFLIVARGGRVIVRA"
     gene            749..1831
                     /locus_tag="VFQ54_00325"
     CDS             749..1831
                     /locus_tag="VFQ54_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEU0163451.1"
                     /translation="MFATKTAGGDGVTGSSDRIDANITIDDTSGIYQRDAFETADASW
                     KDVTLDDVIAARERIMPYLHRTPLLSSATLSRMTNTELSLKAETFQRTCSFKARGALN
                     AALQLTPEERERGVITISAGNHGQGLAFAASIVGTKAVVFMPETAVPTKVNAIREYGA
                     EVVFAKSMAEVFPAMEAYREAHGMKYVGPYSDAAIIAGQGVVGLEILEDMPDVEAVVV
                     PVGGGGLISGVALAIKSQKPDVRIIGVEPEGANIVNKSLKSGKLEHVGVLNTIADGLT
                     APFAGPLSTAVIAHYVDEMVLVNDDDIVTALQTVIERCKILVEPAGAASVAALMSGKA
                     GVAPGTKTVALLSGGNIDRVKLKSIL"
     gene            complement(1880..2509)
                     /locus_tag="VFQ54_00330"
     CDS             complement(1880..2509)
                     /locus_tag="VFQ54_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163452.1"
                     /translation="MTAAAIVAIALVSILAFSGPRETVARWTHVDLSGIRIDIVPWLD
                     HNESITEKDLRLGSAISLADAQSSVPFPILTLPGAGPNGVWLKAGNTVTPGAVVTLLY
                     EADPEYPEIGSTGIGIALMQWSEPNGAVFFIKQVAQTEQFEPVRINGSSGFWIIGGRL
                     LTDPGAPDATSRPSANVLIWTDRNGITYRLESELERDAAVRLAESLGAP"
     gene            complement(2641..>3045)
                     /locus_tag="VFQ54_00335"
     CDS             complement(2641..>3045)
                     /locus_tag="VFQ54_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0163453.1"
                     /translation="RFRPNAPFRPWLLRIVANEAKNRRLATSRRQRHLLPEDRIELIQ
                     DQHRDASPEYLAVTDETSAWLLAHLERLRDEDRIVIICRHVLLLSEAESAAVIGCARG
                     TVKSRLHRAMGRLRASIAAEATITPASKGVAR"
BASE COUNT          570 a          915 c         1027 g          533 t
ORIGIN      
        1 tggccaccgt accgtgcgga gcgtgggtca tcatgacgtc gatggatccg cgtgactctg
       61 gacgtacgtc ggtctcgata ccgcctggat tcatgcgctc ttctctcgtt tgcgtccgga
      121 aagtggggcg acacatctcg ccccagagca actctagggt cggggcgtgg ccgtttcggg
      181 acgagggcat gatgagaaga cggggaaagt gtcacgattc tgtggtgatg ctgggagatg
      241 cgctcaggcg cggacgatga cgcgaccgcc gcgggcgacg atcaggaact cccgggagcc
      301 gcgccggaac tggacctcga cgccggggac gaggtcgaca gtacggacat ccgtaaagac
      361 gatggccgcc gatccggcga tcacatcgac ccggttgagt tccggccaga gcgtgatcat
      421 cgcgacgggt cggccctgcg tcccggggac ggtgagttgc cagacggtcg agctgggcag
      481 ccggaacggt gcgcgctgga ccttcacgtc gaggagctcg gcgatccggg gaacgaccgc
      541 cgcctcgtaa cgcagcgtct cgatgttgcg gttgcgcgag tgctcctgac cgttcggcgt
      601 ggcggtctca tcaggagaat cgttatccgg ggtcatggca tcgtcgatac acgtatgacg
      661 tcacaacggt tggccgcatc cttttgtata ctcaccgctg ggcaacgggc ccgcgtgctt
      721 ttcgacacag agagatgagg gatcgcagat gttcgcaacg aagaccgccg gcggagacgg
      781 agtcaccgga tccagtgacc ggatcgatgc gaacattacg atcgacgata cctcgggtat
      841 ctaccagcgc gacgccttcg agacggcgga tgcctcgtgg aaggacgtta ccctcgacga
      901 cgtcatcgcc gcgcgcgaac gtatcatgcc ctatcttcac cggacgccgc tcctctccag
      961 cgcgacgctg agccggatga cgaataccga gctcagcctg aaggcggaga cgttccagcg
     1021 tacctgctcg ttcaaggcgc gcggcgcgct caatgcggcg ttgcaactga cgccggagga
     1081 gcgcgagcgc ggcgtgatca cgatctcggc cggcaaccac ggccaggggc tcgccttcgc
     1141 ggcatcgatc gttggaacga aggcggtcgt cttcatgccg gagacggcgg tgccgaccaa
     1201 ggtcaacgcg atccgcgagt acggcgcgga ggtcgtcttc gcgaagtcga tggccgaggt
     1261 attccccgcg atggaagcgt accgcgaagc gcacggcatg aagtacgtcg gtccctactc
     1321 cgacgcggcg atcatcgccg gccagggcgt cgttggtctc gagattctcg aggacatgcc
     1381 ggatgtcgag gcggtcgtcg tgccggtcgg cggcggtggc ctgatctcgg gggtcgcgct
     1441 ggcgatcaag agccagaagc cggatgtgcg gataatcggg gtcgagcctg agggcgcgaa
     1501 catcgtcaac aagagcctca agtccggcaa gctcgagcac gtcggcgtcc tgaacacgat
     1561 cgccgacggt ctcaccgcgc cgtttgccgg cccgctttcc acggccgtta tcgcgcatta
     1621 tgtcgacgag atggtgctcg tgaacgacga cgacatcgtc acggcgctgc agaccgtgat
     1681 cgagcgctgc aagattcttg tcgagccggc gggcgcggcg agcgtcgcgg cgctgatgag
     1741 cggcaaggcc ggtgtggcgc cgggtacgaa gacggtcgcg ctcctcagcg gcggtaacat
     1801 cgaccgcgtg aagctgaagt cgatcctgta accgtcggag gacaccccgt gcgagcgatc
     1861 ccaccggaga gcccgtacct cacggcgctc ccagcgattc tgcgaggcgg acggcggcgt
     1921 cccgctcgag ctcggattcg agccggtagg taatgccatt ccgatccgtc cagatcagga
     1981 cgttggcaga tggacgcgag gtcgcatctg gcgcgcccgg atctgtcagg aggcgaccac
     2041 cgatgatcca gaaaccggaa cttccattta tgcggaccgg ctcgaattgt tccgtttgtg
     2101 ccacctgctt gatgaagaag acggcgccat tcggctcact ccattgcatg agcgcgattc
     2161 cgattcccgt cgacccaatc tcgggatact cgggatcggc ctcataaagc agtgtcacaa
     2221 cagcgccggg agtcacggtg tttcccgcct tcagccaaac cccgttcgga ccggcacccg
     2281 gcagggtcaa tatcgggaag gggacggacg attgcgcatc cgcgagagag atcgcggagc
     2341 ccaatcgcaa atccttttcg gtgatcgact cgttgtgatc gagccaaggc acgatgtcga
     2401 tccggattcc ggaaagatcg acgtgcgtcc agcgtgcgac agtctcgcgc ggacctgaaa
     2461 aagcgaggat ggaaaccagg gcgatcgcga cgatagcggc tgcggtcacg atccatcgca
     2521 tacgggcagg ctttcgcgac cgacgcgggg gcagattcgc gcggatcgta gtggcgagat
     2581 cgggtgttgg tgggaacacg gtttcggggc gaagacgttc cagctggcga tcgatgtcgt
     2641 tcatcgggct accccctttg acgcgggagt aatcgtcgct tctgccgcaa tcgaggcgcg
     2701 gagacgcccc attgcgcggt ggagccgcga tttcaccgtt cctcgtgcac agccaatcac
     2761 cgcggcggat tctgcttccg aaagcagcaa cacgtgacga cagataatga cgatccggtc
     2821 ctcgtcgcgt agccgctcca gatgggcgag gagccacgca ctcgtctcat cggtcacggc
     2881 aagatattcg ggcgatgcat cccgatgctg atcctggatc agttcgatcc ggtcctcggg
     2941 aagcaggtgg cgctgtcgac ggctcgtggc gagacgccga ttcttcgcct cgttcgcgac
     3001 gatccgcaag agccaggggc gaaatggcgc gttggggcgg aagcg
//
LOCUS       DASTMD010000024         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2769651, whole genome shotgun sequence.
ACCESSION   DASTMD010000024 DASTMD010000000
VERSION     DASTMD010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2769651"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2229
                     /locus_tag="VFQ54_00340"
     CDS             <1..>2229
                     /locus_tag="VFQ54_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163454.1"
                     /translation="FTVATEPVRKTPESEELATQAREIIVAELRRQYLLPPDAALGRA
                     FAAANGYIYEKTHENLPPDAEIPIQIGATAVVFDGHVATFGHVPPGQLILVEDGLVYA
                     LPELESWLPHYADRQDDGPPVEPLGYGPRIAPIIAQTDLRSGDTILLCSTGFGQAFAE
                     EMADAAKTTSDLGFLHGRDPDRILDVFRDLIVDSGLNDAAVTVVAFAPVAGGKQIRTL
                     GDVGRRARDSFRHGVAGTRHLLPAPRMKSGNPSPAMDGQLAAAAVTTVDPIATLEESG
                     EPVPGQSHRARRSEWRAKSIRVAERLVPTRQTTWEAPTEVRQFGVPGAHGVRIYRGRA
                     SEMGEESWRNRLPRVPVRQAWMWALVSLILVAAIAGGGWARERWFVTTVNFQTSLAAV
                     DQSISDARKIPDPARADAEYLNAQRELGDARNDGAPESDITPRQQAITDGRDKLHNVI
                     RLSDVTRIGGLPPELQGGNTRVVHTAAGKIFLVNGSLYQLQPDTKTIVKVLEEGSKIG
                     NVTVGSLYGVADDTTGLYTTDGTHVFVFSSESQWQVVSLGEINDQGPWPAGSVGAFDG
                     GLYILEAKYKNIYRFDADPDDGKSAAAVDWVQDSYREDLDDAVDLAMDGKIYLLMKDG
                     HVVTFYKGAQETDIAPKYLQLQDKEQAVSIVNGPATNYLYVAVTSDSNPRVVAFDKTD
                     KHEYQLELPAGFSTEGVDVKQPFDGLQAITVDENSGILYLINGDGVWSAWYSLPADAL
                     PP"
BASE COUNT          397 a          723 c          744 g          365 t
ORIGIN      
        1 ggttcaccgt tgccaccgag cccgtgcgaa agacgcccga atccgaggaa cttgctaccc
       61 aggctcgcga gatcatcgtc gccgaattgc gacgtcagta tctcctcccg ccggacgcgg
      121 cgctgggacg ggccttcgcc gcggcgaacg ggtatatcta cgagaagacc catgagaacc
      181 tcccgccgga cgccgagatc ccgattcaga tcggcgccac cgccgtcgtc tttgacggac
      241 atgtcgcgac gttcgggcat gtgccgccgg gacagttgat cctcgtcgag gacggcctcg
      301 tctacgcgct cccggaactc gaatcgtggc tgccccatta cgcggatcgc caggacgatg
      361 ggccgcccgt cgagcccctt ggctacgggc cgcggatcgc accgatcatc gcccagacgg
      421 atcttcgctc cggcgacacg atcctgctct gctcgaccgg cttcgggcag gcgttcgcag
      481 aggaaatggc ggatgccgcg aagacgacct cggacctcgg cttcctgcac ggtcgcgatc
      541 ccgatcggat tctcgatgtc ttccgcgatc tgatcgtcga ctccgggctg aatgatgccg
      601 cggtgaccgt cgtggccttc gcgccggtgg cgggcggcaa gcagatccgg acgctcggcg
      661 atgtcgggcg ccgggcacgg gactccttcc gtcacggggt cgcggggacg cgtcatcttc
      721 tgcccgcccc tcgcatgaag tcgggaaacc catcgcccgc catggacggc cagctggccg
      781 cggcggcggt gacgacggtg gatccgatcg ccacgctgga ggagagcggg gaacccgtcc
      841 ccgggcaatc gcatcgggcg cggcgatcgg agtggcgcgc gaagtcgatc cgggtagccg
      901 agcgtctggt accaacacgg cagacgacct gggaggcgcc gacggaagtc cgccagttcg
      961 gggtgccggg tgcgcacggc gtgcgtatct atcgcggacg ggcgtcagag atgggggagg
     1021 agtcgtggcg gaatcgtctg ccgcgggttc ccgtccgcca ggcctggatg tgggcgctcg
     1081 tctcgctgat cctcgtcgcc gcgatcgcgg gcggaggatg ggcgcgcgag cgctggttcg
     1141 tcacgaccgt caacttccag accagccttg ccgcggtcga ccagagcatc tccgacgcgc
     1201 gcaagatccc ggatccggcg cgagccgatg ccgagtacct gaacgcccag cgtgagctcg
     1261 gggatgcccg caacgacggc gcgccggagt cggacatcac accacgccag caggcgatta
     1321 ccgatgggcg cgacaagctc cataacgtga tccggctcag cgacgtgacc cggatcggcg
     1381 gcttgccacc ggagttgcag ggcggcaaca cgcgcgtggt gcataccgcg gccggaaaga
     1441 tcttcctcgt caacggcagt ctctatcagc tccagccgga tacgaagacg atcgtcaagg
     1501 ttctcgagga aggctcgaag atcggcaacg tgaccgtggg atcgctctat ggcgtcgccg
     1561 acgacacgac cggtctctat acgaccgatg gcacgcacgt cttcgtcttc agctccgaga
     1621 gccaatggca ggtcgtttcg ctgggagaga tcaacgatca gggaccgtgg ccggccggat
     1681 cggtcggcgc cttcgatggc ggtctctaca ttctcgaggc gaaatacaag aacatctatc
     1741 gcttcgatgc cgatccggat gacgggaagt ctgccgccgc cgtcgactgg gtgcaggaca
     1801 gctaccgcga ggatctcgac gacgcggtgg atctcgcgat ggatggcaag atctatctcc
     1861 tgatgaagga cggtcacgtc gtcacgttct acaagggcgc gcaggagacg gacatcgcgc
     1921 cgaagtacct gcagctgcag gacaaggagc aggccgtctc gatcgtgaac ggccccgcca
     1981 cgaactacct ctacgtggcg gtgacgagcg attccaatcc ccgcgtcgtg gcattcgaca
     2041 agacggacaa gcacgagtac cagctcgagc ttccggcggg attctccacc gagggcgtcg
     2101 acgtcaagca accgttcgat ggcttgcagg cgatcacggt cgacgagaac tcggggattc
     2161 tctatctcat caatggcgat ggagtctggt ccgcctggta cagcctgccc gccgatgccc
     2221 tgccgccgg
//
LOCUS       DASTMD010000025         1699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_537995, whole genome shotgun sequence.
ACCESSION   DASTMD010000025 DASTMD010000000
VERSION     DASTMD010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537995"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..795)
                     /locus_tag="VFQ54_00345"
     CDS             complement(61..795)
                     /locus_tag="VFQ54_00345"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEU0163455.1"
                     /translation="MLNLLFTVVIPVIILTRFGSLEAFLVALAFPIGFGIYEVVRNRR
                     ISWQSGLGIFSLLLTGGFKILHLPPEWVAVKEAMVPSVLALAVLVSAWVGRPLARLFL
                     NQILDREKIDAALAERGNVHEYERRTSIATYLLAFTFLLSATLNFILAKVIVTSDPST
                     SAYNHEIGRMTALSFPVITIPVMVSMFVTIIYIMWTVSKLTGLEAESVIKQQPKKPKR
                     GASTADQTPLPAADIIDVDGRTTGAA"
     gene            996..>1699
                     /locus_tag="VFQ54_00350"
     CDS             996..>1699
                     /locus_tag="VFQ54_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU0163456.1"
                     /translation="MSSTFERYKRAAARLDELIEHSSAPEDKSREAVQARAELRMGRL
                     RAFLRHFGSPQDRYPIVHVGGTSGKGSTSTTIAAILTAAGYRTGLHISPYLQTPLEKL
                     QLNGNLIDPEHFTSLVDRLLAAHEAWIGGGGEPLTYGELWMALTVFFFADAGVEVAVL
                     EVGAGGRFDLTNVITPAVSVITSVGIDHTNTLGSTIEEIAWHKAGIIKRGIPAVTAAT
                     HPVALAIIRAEASAQG"
BASE COUNT          323 a          501 c          573 g          302 t
ORIGIN      
        1 gcgtcgtccc ggaaccgcga caagcggagt ggggagggaa ggcgtgggga tgtcccgact
       61 ctacgcggcg ccggtggtgc ggccgtcgac gtcgatgatg tccgccgcgg gaagaggagt
      121 ctgatcggcg gtggatgcgc cgcgcttcgg cttcttcggc tgctgcttga tcaccgattc
      181 cgcctcgagg ccggtcagct tgctcaccgt ccacatgatg tagatgatcg tgacgaacat
      241 cgagaccatc accgggatcg tgatcaccgg gaagctgagc gccgtcatcc ggccgatctc
      301 gtggttgtag gcactggtgc ttggatcgct ggtgacgatg accttcgcga gaatgaagtt
      361 cagggtcgcc gaaagcagga aggtgaaggc gagcaggtag gtcgcgatcg atgttcgtcg
      421 ctcgtattca tggacgttgc cacgctcggc gagggcggcg tcgatcttct cgcgatcgag
      481 gatctggttg aggaagagtc gcgccagcgg gcgaccgacc cacgcggaaa ccagcacggc
      541 gagggcgagg acggaaggaa ccatcgcttc cttgaccgcc acccattccg gcggcaggtg
      601 caggatcttg aaaccaccag tcagcaggag actgaagatg ccgagcccgg attgccagct
      661 gatgcggcga ttgcggacga cctcgtagat gccgaagccg atcggaaacg ccagcgcaac
      721 caggaaggct tcgagcgatc cgaagcgggt caggatgatg accgggatga cgacagtgaa
      781 gagcaggttc aacatgcagg gatatcctgt cgagtaatga tcaggtgatt ctggcacggt
      841 gatgcgcggg catcgctgcg caatcaccat catacgatct tccacgaacc gtcgcgatgg
      901 ggaatcccgt tcccggaggg tgtcgatcgg tacggagacg cgtatcatgc ctcggtattt
      961 gccggttccg accttcacgg agtatcaggg aacgcatgag ttcgaccttt gaacggtaca
     1021 aacgcgccgc cgctcgtctc gacgagctga tcgaacattc ctcggcgccg gaggacaaat
     1081 cgcgggaggc ggttcaggcc cgggccgagc tccgcatggg acggttgcgt gcgttcctgc
     1141 gccacttcgg gagcccccag gatcgctacc cgatcgttca tgtcggcggg acatccggca
     1201 agggatcgac gagcacgacg atcgccgcga tcctgacggc cgccggatac cgcaccggcc
     1261 tccacatctc tccctatctc cagacaccgc tcgaaaagct ccagttgaac gggaatctga
     1321 tcgatccgga gcatttcact tcgctcgtcg accggctcct ggcggcacac gaagcctgga
     1381 tcggtggtgg cggcgagcca ctgacctatg gcgagctctg gatggcgctc acggtcttct
     1441 tcttcgccga tgctggcgtg gaggttgcgg tgctcgaggt cggcgccggt ggtcgcttcg
     1501 atctcacgaa tgtcatcacg cccgccgtca gcgtgatcac gtcggtcggg atcgatcaca
     1561 cgaatacgct cgggtcgacg atcgaggaga tcgcctggca caaggcggga atcatcaagc
     1621 gggggatccc cgcggtcacc gcggcgaccc atcccgtggc gctcgcgatc atccgggcgg
     1681 aggcgtcggc gcaggggag
//
LOCUS       DASTMD010000026         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1162466, whole genome shotgun sequence.
ACCESSION   DASTMD010000026 DASTMD010000000
VERSION     DASTMD010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162466"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VFQ54_00355"
     CDS             <1..812
                     /locus_tag="VFQ54_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU0163457.1"
                     /translation="PPWAGCHRSGGGAMNRVEVVGFPVDIIDVAGLRAWLAASLQPDG
                     PCRHLVTLNPEYVMQARRDPGFAVAIRAADLVVADGVGVSKAASMLLAAQGRSSRVER
                     IPGVEIVETIARMPGARVFLLGAGPGVAARAASRLEERHPGAIVAGVWDGGTAEARDD
                     DESLRRIREAAANVVLVAYGASGQVAWVVRNQRALGEAGVRLAIGVGGAFDFISGEVA
                     RAPEFVRRLGLEWLYRLVREPWRWRRQLVLPGFALLALREIAGARITARRS"
     gene            929..1531
                     /locus_tag="VFQ54_00360"
     CDS             929..1531
                     /locus_tag="VFQ54_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HEU0163458.1"
                     /translation="MASQPSFSDYRTSGQVPCSYHPNVMTGLRCSRCGKPICPLCAVR
                     TPVGLRCPDCAGVRGLPSYRTPSTDLVKSVGLGLVIAVAVAVLWRFFPNWEFYLCLLL
                     GFGAVETMAKVANYKRGVDLQAAAIGVVVVGMVLARVLVAQRFGITLSDVNNLNDLVA
                     TQAAYREYGIVSVATILQLKLIPDLIYAAIPVAIAWYRFR"
     gene            1561..>2777
                     /gene="murJ"
                     /locus_tag="VFQ54_00365"
     CDS             1561..>2777
                     /gene="murJ"
                     /locus_tag="VFQ54_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEU0163459.1"
                     /translation="MVDADAVVAAIPVRDGSEVPGRGVSGAIAVAAIIIMAGNVLSRV
                     LGLVREQLAASLFGTGDKIAAFTVADNLQTLLFDLMVSGALQAALIPVLAQWARSGDG
                     AKEELRRVSGALLTIVALVLGSLVTIGVVFAPHLVDGLIWFSGDDTARGEGTIHLAIR
                     LVRIVLPAAALLGIGAILQAVLYAMGRITAPALSTAFRNLAIVTSAAIFGSRFGVESM
                     AWGTVVGAVLVVALQIVPLIRAGALPRPNFGFRHPALREMLGLYLPVFLGLFVSSFAV
                     IVDRGLAWGAGEDAVGAMRYATTLVQLVLGIIAAAISLAALPTLSRHFSADDDASFRR
                     VLGRALTVVTILIFPATFGLAALARPIADLLFGQGATDQHGVNQIAIALVGYLPGTLA
                     AAYDQVLIFAFYSR"
BASE COUNT          351 a          906 c          967 g          553 t
ORIGIN      
        1 tcccgccttg ggctggctgc catcgctccg gaggcggcgc gatgaaccgc gtggaagttg
       61 tcggatttcc ggtcgacatc atcgacgtag ccggattgcg ggcgtggctg gcggcgtctc
      121 ttcagccgga cgggccgtgc cggcatctcg ttacgctcaa tcccgagtat gtgatgcagg
      181 cgcgtcgcga tccgggattc gccgtggcga tccgcgccgc ggatctcgtc gtcgcggatg
      241 gggtcggggt gtcgaaggcg gcatccatgc tgctcgccgc gcagggacgc agttcccggg
      301 tcgagcggat tccgggcgtg gagatcgtcg agacgatcgc caggatgccg ggagcccggg
      361 tcttcctcct cggcgccggt ccgggagtcg ccgcgcgggc ggcctccagg ctcgaggagc
      421 gacatcccgg ggcgatcgtc gctggtgtct gggatggcgg gacggcggag gcccgcgacg
      481 acgatgaatc gctgcgtcgc atccgcgaag ccgccgcgaa cgtcgtgctc gtcgcctatg
      541 gcgcgagtgg gcaggtcgcg tgggtcgtcc gcaaccagcg ggcgctggga gaggcgggtg
      601 ttcgcctcgc gatcggtgtc ggcggcgcct tcgacttcat ctcgggggag gttgcgcgag
      661 cgccggagtt cgtgcgccgc ctcggtctcg aatggctcta ccggctggtg cgcgagccgt
      721 ggcggtggcg acgacaactg gtattgccgg ggttcgcctt gcttgcactc agggaaatcg
      781 ccggggcgag gatcaccgcg cggcggtcgt gaacgcctgc gagtcgaaga tgcggggtag
      841 cgtatgattt gtctcgtcat tctgcacgag acagcgccgc ccgcgcctgt caacgttacg
      901 ctcttcatgg aaagggccct cctcctctat ggcgtcgcag ccatccttct ctgactaccg
      961 caccagcggc caggttccat gcagctatca tccgaatgtc atgaccgggc tccgctgctc
     1021 gcgctgcggc aagccaatct gcccgctctg cgcggtgcgc acgccggtcg gtctccgatg
     1081 cccggattgc gccggggttc gcggtctccc gagctaccgg acgccgtcga ccgatctcgt
     1141 gaagtccgtc ggtctcgggc tggtgatcgc cgtcgcggtc gccgtgctct ggcgattctt
     1201 cccgaactgg gagttctatc tctgcctcct cctcggcttc ggcgccgtcg agacgatggc
     1261 gaaggtggcg aactacaaac gtggtgtcga tcttcaggcg gcggcgatcg gcgtcgtggt
     1321 cgtggggatg gtgctcgccc gtgtcctggt cgcgcagcgg ttcgggatca cgttgtcgga
     1381 cgtgaacaac ctcaatgatc tcgtcgccac gcaagccgcc tatcgtgaat acggcatcgt
     1441 gtcggtagcg acgattctcc agctcaagct gattccggat ctgatctacg ccgcgattcc
     1501 ggtcgccatc gcctggtacc ggttccgata atggcttccc cgccaccgcc ggacatcgag
     1561 gtggtggacg cggatgcggt cgtcgctgcc atcccggtca gggatggcag cgaggttccg
     1621 ggacgcgggg tgagcggggc gattgccgtc gccgcgatca tcatcatggc ggggaatgtc
     1681 cttagccgtg tccttgggct cgtgcgtgaa cagctcgcgg cgagcctctt cggcaccggc
     1741 gacaagatcg ccgccttcac cgttgccgac aatctccaga cccttctctt cgatctgatg
     1801 gtgagcggtg ccctccaggc cgcgctgatt cccgtgctcg cccagtgggc gcgatccggt
     1861 gacggcgcga aggaggaact gcgtcgcgtc agcggtgccc tgctcacgat cgtcgcgctc
     1921 gtgctcggct cgctggtgac gatcggcgtc gtcttcgcgc cccatctcgt cgatggactg
     1981 atctggttct ccggagatga cacggcgcgc ggcgagggga cgatccacct tgcgattcgt
     2041 ctcgtgcgga tcgtgctgcc ggcggcggcg ctgctcggga tcggggcgat cctccaggcc
     2101 gtgctctatg cgatgggtcg gatcaccgct ccggcgctct cgacggcgtt ccgcaatctc
     2161 gcgatcgtca cctcggcggc gatcttcgga agccggttcg gtgtcgagag catggcgtgg
     2221 ggcacggttg ttggcgccgt cctcgtcgtc gcccttcaga tcgtgccgct gatccgggcc
     2281 ggggcgctgc cgcgaccgaa cttcggtttt cgccatcccg ccctgcgcga gatgctcggt
     2341 ctctatctcc ccgtctttct cggtctcttc gtcagcagct tcgcggtgat cgtcgaccgg
     2401 gggctcgcct ggggagccgg ggaagacgcc gtcggggcga tgcggtacgc gacgacgctc
     2461 gtccagctcg tgctcgggat catcgcggcg gcgatctcgc tcgcggcgct gccgaccctc
     2521 tcccgtcact tctcggcgga tgacgatgcg tcgtttcgcc gcgtgctggg tcgggcgctg
     2581 acggtggtca cgatcctgat cttcccggcc acattcggac tggcggcgct ggcgcgcccg
     2641 atcgccgacc tcctcttcgg acagggtgcg accgatcagc atggggtcaa ccagatcgcg
     2701 atcgcactgg ttggctacct cccggggacg ctcgcggccg cctacgacca ggtgctgatc
     2761 ttcgccttct attcacg
//
LOCUS       DASTMD010000027         7066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2501719, whole genome shotgun sequence.
ACCESSION   DASTMD010000027 DASTMD010000000
VERSION     DASTMD010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2501719"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..581)
                     /locus_tag="VFQ54_00370"
     CDS             complement(45..581)
                     /locus_tag="VFQ54_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163460.1"
                     /translation="MSTDAAATATTTTNSIPVEVFRNVLLSILDEAYVGTANSWTLFT
                     DKGTSIFETLSTIDAVTASTVFSDRASNIAAQVNHVRFHLDVLSDGLKNGWGTSADWP
                     GSWKIGEVSEEQWQDLVNRLRAAYEETRAVAASWNDWNEDFVSGAFSIVAHAAYHLGE
                     IRLSLAALNVPVAPDVAS"
     gene            884..1876
                     /locus_tag="VFQ54_00375"
     CDS             884..1876
                     /locus_tag="VFQ54_00375"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU0163461.1"
                     /translation="MTPTTIPLNPAERSTLIGNRRARGVPDDAIHALLRRWLPDRAVR
                     FSRAETGGSTPVYQVTTEGETLFLRLAEEPGERRDAEVAVHRLLRARGVAVPGILSWE
                     PDPPELDRSAVLTTAMPGRPLGQRPDLPAETIQLALKAAGRDLARINRIPVRGYGWAD
                     TTDPRDDTPIAEHATRTLWTAEYARTTEEILAAGLLSGGFASELERAIDRWLTVPDPG
                     PSSLAHGDFDPTHIFVSASGEYTGIIDFGEIRGADPLYDLGYVMLHDQQAAQAPILPA
                     ILAGYREVTPLPEDVLPLIRDQAIAIGVRMLAISRRRNAPWADLAAQIERLLDG"
     gene            complement(1969..2808)
                     /locus_tag="VFQ54_00380"
     CDS             complement(1969..2808)
                     /locus_tag="VFQ54_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU0163462.1"
                     /translation="MKIYAHRGASIEFPENTLAAFNRAIELGIEGIELDVYLSKDGIP
                     VVIHDPTLDRTTNGTGPIDSLTAAELGALDAGNGEYVPTLAEVLSLVAGKLHVNIEIK
                     TAIAADAVLEEVKNHPTLEWVTSSFVWEALEHVHNVAPDVDCWPLTIGGPQKLDALIA
                     IAAPHYPDADRNIRQMWPNPPTIEASIDAAAKWGGSAISVWEHDLTADDIAAIHARNQ
                     IAWVWTVNDADRARELMAIGVDSLCTDDPAMALRVVAEAKQAVVEVLFAAAPGSLRQG
                     CSA"
     gene            complement(3303..3695)
                     /locus_tag="VFQ54_00385"
     CDS             complement(3303..3695)
                     /locus_tag="VFQ54_00385"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0163463.1"
                     /translation="MIHHIELWVPDFARAVASWEWLLAELGCRRYQEFAGGVSWRLPA
                     TGTYIVLEQSPDMLAIGHDRHRPGLNHLAFDAGSREVVDRLTAESKGHGWSLLFADRH
                     PFAGGPDHYAAYLEDQDGFEVELVAGDS"
     gene            3935..4972
                     /locus_tag="VFQ54_00390"
     CDS             3935..4972
                     /locus_tag="VFQ54_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0163464.1"
                     /translation="METSSITTTPGHTASKPTVPPAAGTKIRSTYTAAASASSASDLV
                     IETSALTKRYGALTAVDNLELAVRRGEVYGFLGPNGAGKTTTLRMLLGLIAPTSGTVR
                     VLCTPAGTSSSLAQVGAMIETPAFYPFLSGEDNLRILARYSGVSEKAIGPVLEQVDLL
                     GRGGDAFKTYSLGMKQRLGVAAALLKDPALLILDEPTNGLDPAGMAEMRTLIRDLGQG
                     NRTVLLSSHLMSEIEQISDRVGIIFRGRIVAEGTVDQLRGADALYLRVDAPGKAKEML
                     VKLAGVEHVHQDGDSLRIDSIDSLGLDPAYVNQRLVFGGIAVRELRLEQASLESAFLA
                     LTNPDQEAPHV"
     gene            4965..5867
                     /locus_tag="VFQ54_00395"
     CDS             4965..5867
                     /locus_tag="VFQ54_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0163465.1"
                     /translation="MSSLVATGIGTRSSMNPVRTMLRDIQTELMLLGKRPSTWVLLGV
                     WLALSQIFAYIFPYVTYRNDSTATAPGGAPLLPTLLPDQLVSALVGGFAFYGGTIALI
                     LGVLTFGSEFGWNMWKTLFTQRPGREHIFLAKAIAMGIVLLPFGLIEFALGGVVSTII
                     ATIEGQTISAPSFMHLVEAIGSGWLIMMVWAGIGMLLAVITRGTSLAIGLGILYALVV
                     EGMISAFANSISWLKPIVEGFLRANSYSLIEPINASTSQANGGPGSFDGSFVDPLQAF
                     IVLAGYLVISVVASMWLMRRRDIA"
     gene            6142..6570
                     /locus_tag="VFQ54_00400"
     CDS             6142..6570
                     /locus_tag="VFQ54_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163466.1"
                     /translation="MQIANRPVPATTGNAVVLIYRLLASALALGVLVESVFASQGLFN
                     SKPEMIDGHRRLGNLLGVVTVLTLGVAIYCWRKGRFSPAPMIRGTVIVVLIVIQIILG
                     YATRDSTNAIVWHIPTGVALMGLATANAALAWVTPTARRA"
     gene            complement(6631..7014)
                     /locus_tag="VFQ54_00405"
     CDS             complement(6631..7014)
                     /locus_tag="VFQ54_00405"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEU0163467.1"
                     /translation="MSAASPTPESIRHALVTDRVIDITTTGRASGKEHRIETWFHNLD
                     GQVYLTGSPGKRDWYANLVANPDFTFHLKSVGADLPAHATAVTDPAQKREIFTVLLAN
                     LDRSQDIDTWVEKSPLVAVDLPTVG"
BASE COUNT         1242 a         2333 c         2221 g         1270 t
ORIGIN      
        1 agagcgtatc cgtggtcgtt tcggtcatca gggatctccg ttcgtcagct ggcgacatca
       61 ggcgcaacag ggacgttgag cgcggcgagc gagagacgga tctcgccgag atgataggcg
      121 gcgtgggcga cgatgctgaa ggcgccgctc acgaagtcct cgttccaatc gttccaggat
      181 gccgcaaccg cacgcgtctc ctcataggcg gcgcgaaggc gattgacgag atcctgccat
      241 tgctcctccg agacctcgcc gatcttccac gagccgggcc aatcggccga cgtcccccag
      301 ccgttcttga gtccgtcgct caggacatcg aggtggaagc ggacgtggtt gacctgcgcc
      361 gcgatattcg acgcgcggtc ggagaagacc gtcgacgcgg tcaccgcatc gatcgtgctc
      421 agtgtctcga agatcgaggt gcccttgtcg gtgaagagcg tccacgaatt cgcggtcccg
      481 acgtacgcct catcgaggat ggaaagcagg acgttgcgaa agacctcgac cgggatcgag
      541 tttgtcgtgg tggtggcggt agcagcggca tccgtggaca tcagcgtctc ctccgtgatc
      601 gaagcgttga aacaggattt ctgggcaggg acccgatggc cccgacggta cgccgtgatc
      661 ctaccgtcga tggcggacac ttcccgtcct gatttcatgg agaacgcgga gcgtccgacc
      721 tcgtgagatc ctgcgggaca cccagaccga caaggcgcac tcctgcggcg cgtcctccga
      781 gcttgtcgag agatcagttg gtcacactac gtgccccagc cgcaacccgc cgtcgagccc
      841 gtcaagaaat cgcccctgta aggtccacat ggatggccac ataatgacgc caacaacgat
      901 tccgctcaac cctgctgaac ggagcacgct tatcggtaat cggcgagcac gaggagtacc
      961 cgacgatgcg atccacgcgc tgctcaggcg ctggctgccc gatcgcgccg tgcggttctc
     1021 ccgagcggag accgggggtt cgacgcccgt ctaccaggtg acgacggaag gcgagacgct
     1081 cttcctccgt ctcgccgagg aaccgggaga acgccgcgat gccgaagtcg cggtccatcg
     1141 cctgctccgg gctcgcggcg tcgcggtgcc cggaatcctg agctgggagc cggatccacc
     1201 cgaactcgat cgctcggcgg tgctgacgac cgcgatgccg ggtcggccgc tcggccagcg
     1261 tccggatctc ccggccgaga cgatccagct cgcactcaag gcggccggtc gcgatctcgc
     1321 ccggatcaac cggataccgg tgcgaggcta cggctgggcc gatacgaccg atccgcgaga
     1381 cgacacgccg atcgccgagc atgccacgcg cacgctctgg accgccgaat acgcacgcac
     1441 caccgaggag attctcgccg ccggtcttct ctcgggcgga ttcgcgtcgg aactggagcg
     1501 tgcgatcgat cgctggctaa cggtgccgga tcctggtcca agttcgctcg cgcatggcga
     1561 tttcgacccc acgcacatct tcgtctcggc atccggtgaa tacacgggga tcatcgactt
     1621 cggcgagatt cgtggcgcag atccgctcta cgatctcggg tacgtcatgc ttcacgacca
     1681 gcaggcggct caagcaccga tcctccccgc catcctcgct ggatatcgcg aggtgacgcc
     1741 gcttccggag gacgtgcttc cgctgattcg cgatcaggcg atcgcgatcg gtgtccgcat
     1801 gctggcaatc tcccggcgcc gcaacgctcc gtgggcggat ctggccgcgc agatcgaacg
     1861 cctcctcgac gggtgattat ccatctgaca ggagcccaca ggggtcgccg cgacggatgc
     1921 tcaatgggga cgcggggtca ccacaagggt gacccctacg acattacgtc atgccgagca
     1981 gccttgccgg agcgacccag gtgccgccgc gaataacacc tcgacgacgg cctgcttcgc
     2041 ctcggcgacg acgcgcagcg ccattgccgg atcgtcggtg cagagactgt cgacaccgat
     2101 cgccatcagt tcacgggcgc gatccgcgtc gttgaccgtc cacacccagg cgatctggtt
     2161 gcgggcatgg atcgcggcga tgtcgtcggc ggtcaggtcg tgctcccaga ccgagatcgc
     2221 cgagccaccc cacttcgcgg ccgcgtcgat ggaagcctcg atcgtcggcg ggttcggcca
     2281 catctggcgg atattgcgat cggcatcggg gtagtgcggg gcggcgatcg cgatcagcgc
     2341 gtcgagcttc tgcggtccac cgatcgtcaa cggccagcag tcgacatccg gcgcgacatt
     2401 gtggacgtgc tccagcgcct cccagacgaa ggacgacgtc acccactcga gcgtcgggtg
     2461 attcttgacc tcttcaagga cggcatccgc ggcgatcgcg gtcttgatct cgatgttgac
     2521 gtgcagcttc ccggcgacca gcgacaggac ctcggccagc gttggcacat actcgccgtt
     2581 gcccgcatcg agcgcaccga gttcggcagc ggtcagcgag tcgatcgggc cggtgccgtt
     2641 cgtcgtgcga tcgagcgtcg gatcatggat gacgacggga atcccatctt tggagaggta
     2701 gacgtcgagc tcgatgcctt cgatgccgag ttcgatcgcg cggttgaagg ccgcgagggt
     2761 attctccggg aactcgatcg atgctccgcg gtgggcgtaa atcttcatgg aatcctcaat
     2821 cgatacaggt gaagggttga ttcgaaaacg tcgccgggcg aacgtgctgc cgtagcgacc
     2881 ggcgccagca taggccagcc cgattccgta ccgtgttatc agggcatgaa ggccgggtat
     2941 gaggaaccga aaaatcggtc ccacgcgtgg cgttcaccgc catcccgagt agggatgggt
     3001 gcggagtggc cgcaggctcg gaccacgtgt ctctccgttg gttgcgcacc attcgcgtgc
     3061 cgtcaggacc gttgggaacc ggagacgcgg cccgtgcggc cgccacggat cggcagtgcc
     3121 gatccgtgtc cccggaacgt cttgcaccgc gtgtttcggt tggcggccgc ctgcggcgga
     3181 tgtgcttcgc acggggattt ctcgacaagc tcgaaatgac aaatgtgtat gcgaaagcgt
     3241 tggcatgaag cccccgcgca cgcgatcgcc tccaccacgc caacctccgg agtggcaacg
     3301 ggtcaggaat cgccggcgac gagttcgacc tcgaagccat cctggtcctc gagatatgcg
     3361 gcgtagtgat cgggaccacc ggcgaacggg tggcgatcgg cgaagagaag gctccatccg
     3421 tgccccttcg attcggcggt gaggcgatcg accacctccc tgctccccgc gtcgaatgcc
     3481 agatggttga gtcccggccg gtggcgatcg tgaccgatcg cgagcatgtc cggcgattgc
     3541 tcgagcacga tgtaggttcc ggtcgcagga agacgccagc tcacgccacc ggcgaactcc
     3601 tggtagcgcc ggcatccgag ttccgccagc aaccattccc aggacgcaac cgcgcgtgcg
     3661 aaatcgggca cccagagctc gatgtgatgg atcatgacgc tccccgctct cctgccatcc
     3721 agccgtgacg accacccgca gccgaaaagt ccatgccgcc cagcgtatgc gccccgggcg
     3781 ctcgtccaca agtcacccca cgcgttcacg gaatcctcac aacctgcaca cggggcttgc
     3841 acatctgtct tgttgaatgg agtggtgggc gaggaaggga attcagccac tctccggatc
     3901 cgcagcttgc agctcccagg acaaggaaga gccaatggaa acgtccagca tcactacaac
     3961 accaggccac acggcatcaa agccgaccgt gcctcctgcc gcgggcacga agatccgctc
     4021 gacctatacc gccgccgcat ccgcgtcgtc cgcatccgac ctcgtgatcg agacgagcgc
     4081 gctcacgaag cgatacggcg ccctgaccgc ggtcgacaat ctcgaactcg cggtcaggcg
     4141 cggcgaggtc tacggcttcc tcggtccgaa cggcgccggc aagacgacga cgctgcggat
     4201 gctgctcggc ctgatcgcgc cgaccagtgg cacggtgcgt gtgctctgca cacccgccgg
     4261 aacgtcgtcc agcctcgccc aggttggcgc gatgatcgag accccggcct tctatccctt
     4321 cctctccggc gaggacaatc tccggattct cgcccgttat tccggggttt ccgagaaggc
     4381 catcggtccg gtgctcgagc aggtcgatct gctgggacgc ggtggcgatg ccttcaagac
     4441 ctactccctc ggcatgaagc agcgcctcgg cgtcgcggcg gccttgctca aggatccggc
     4501 actcctgatc ctcgacgagc cgacgaacgg gctcgacccg gccggaatgg cggagatgcg
     4561 cacgttgatc cgcgatctcg ggcaaggcaa tcgcacggtc ctgctctcca gccacctgat
     4621 gagcgagatc gagcagatca gcgaccgcgt cggcatcatc ttccgcggca ggatcgtcgc
     4681 cgaagggacc gtcgatcagt tgcgcggcgc ggatgcgctc tacctccggg tcgacgcgcc
     4741 agggaaggcg aaggagatgc tcgtgaagct ggccggtgtc gagcatgtcc atcaggacgg
     4801 cgattccctg cgcatcgaca gcatcgattc cctcgggctc gatccggcct acgtgaacca
     4861 gcgcctcgtc ttcggcggga tcgccgtccg cgagctacga ctcgagcagg cgtccctcga
     4921 atcggccttc ctcgccctca cgaacccgga tcaggaggct ccccatgtct agtctcgtcg
     4981 ccaccggcat cggcacgcgt tcgtccatga accctgtccg gacgatgctc cgcgatatcc
     5041 agaccgaact gatgctgctc ggcaagcgac cgagcacgtg ggtcctgctc ggcgtctggc
     5101 tcgcgctcag ccagatcttt gcctacatct ttccgtatgt gacctatcgc aacgattcga
     5161 cggcgaccgc accgggcggt gcgccgctcc tgccgacgct gcttccggat cagctcgtct
     5221 cggcgctcgt cggcggcttc gccttctacg gcggcacgat cgcgctgatc ctcggcgtgc
     5281 tcaccttcgg cagcgagttc ggctggaaca tgtggaagac gctcttcacc caacgaccgg
     5341 gtcgcgagca catcttcctc gccaaggcga tcgcgatggg gatcgtgctc ctcccgttcg
     5401 ggctgattga attcgcactc ggtggcgtcg tcagcacgat catcgcgacg atcgagggtc
     5461 agacgatctc ggcgccgtcg ttcatgcatc tggtggaagc gatcggttcc ggttggctga
     5521 tcatgatggt ctgggccgga atcgggatgc tcctcgccgt catcacccgc ggcacctcgc
     5581 tcgcaatcgg tctcggcatc ctctacgccc tggtggtcga ggggatgatc agcgccttcg
     5641 cgaacagcat cagctggctg aagccgatcg tcgaaggctt cctccgggcc aactcctact
     5701 cgctgatcga gccgatcaat gccagcacct cgcaggcgaa cggcggaccg ggtagcttcg
     5761 acggatcgtt cgtcgatccg ctccaggcgt tcatcgtcct cgccggctac ctcgtgatct
     5821 cggtcgtcgc ctcgatgtgg ctgatgcgcc gccgcgacat cgcctgaccc cgacttccac
     5881 gctggggacg aggtcgcgtg acctcctccc ggcgcccacc gaccatccgt ccccacggcc
     5941 ctcgtggccg ttcctcccca ggtcgggggc aacgcgtgac ctcgaacgcg aaccacgaga
     6001 agccaccccg gtgttcgcct gttctcgttg cgatgcttct cctctgactc cgtgaaatgt
     6061 cacatcgcgt cgctctgccc cgtcctaccc aggaaggcat cggcgtgaaa acgatgtgaa
     6121 cgacgtcacg aggggaaatg catgcagatc gcgaatcgcc cggtaccggc tacaaccggc
     6181 aatgccgttg tcctcatcta ccgcctgctg gcatctgccc tggccctcgg cgtactggtg
     6241 gaatccgtgt ttgccagcca ggggctcttc aattcgaaac cggagatgat cgacgggcat
     6301 cgccggctcg gcaatctgct cggcgtggtg accgtcctca cgcttggcgt cgccatctat
     6361 tgctggagaa aaggtcgctt cagtcccgcg ccgatgatcc gggggacggt gatcgtcgta
     6421 ttgatcgtga tccagatcat cctcggctat gcgacgcgag atagcaccaa tgcgatcgtg
     6481 tggcatatcc cgaccggcgt cgcactcatg gggctcgcca ccgcgaacgc ggcgctcgcc
     6541 tgggtgacac cgacggcacg acgggcgtag caatcgaaaa ggcgggacac ggaggtcccg
     6601 ccgtactcac gaccgcgtca tcggggcacg ttaccctacc gtcggcaggt cgacagccac
     6661 aagcggactc ttctccaccc aggtgtcgat atcctgtgaa cgatcgaggt tcgcaagcag
     6721 cacggtgaag atctcgcgct tctgcgcggg atcggtgacg gcggtcgcat gcgcgggcag
     6781 atcggcgcca acgctcttca ggtggaaggt gaagtcggga ttcgcgacga gattggcgta
     6841 ccagtcgcgc ttgcctggtg atccggtgag gtagacctgg ccatcgaggt tgtgaaacca
     6901 ggtttcgatg cgatgttcct tgccactggc gcggccggtc gtcgtgatgt cgatcacgcg
     6961 atccgtcacg agcgcgtgac gaatggactc cggggttggc gatgctgccg acatggatct
     7021 ctcctcctga cgggaagcgg atatcaatgt tctcatcata gggtgc
//
LOCUS       DASTMD010000028         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_894750, whole genome shotgun sequence.
ACCESSION   DASTMD010000028 DASTMD010000000
VERSION     DASTMD010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894750"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2029
                     /locus_tag="VFQ54_00410"
     CDS             <1..2029
                     /locus_tag="VFQ54_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0163468.1"
                     /translation="IPMHHFPTSYTYHQWDGTGDEFLNPEDMMESLSEDLLHHGDIDL
                     ALQRAFRWGFQNRDGQHSDGLRDLLNRLRQQRQEMLDRWDFNSITEDIQERLDQILDQ
                     ERSTIQEKKAQLDRQAAVDSALDTDAAKQFLDRKETSLEDLPESTAAQIQQLREYGFV
                     DQQAQADFDKLLQELQQQVADSLFNNMKGAMGGGEDGSGSMEEMKEFLKDINDAFEAQ
                     RNGEQVDLDKLNNRWANQMGGRIDSLEELAQRMRQRLAAAQAMMNMLSPQQRADLMKM
                     MQQAMQDAGLAGELGRLQENLGPIRAPRGLQQSDAHGAEPLSIDMATSILQQANQMGR
                     LENQLRSVSRLEELDGIDPKLLDEVLNDEDKAWLDRWQHIEQELQDSGYVNQTDRGLE
                     LTPRAVRKIGEKALADIFGQLKQNGIGDHDLDNRGVGGELADTTSQWEFGDPFQLDLA
                     KSVMNAIMREGPGAPVHLRPDDFEVVDRDAKTSTATVLLVDMSRSMIHNGCWEAAKRA
                     ALALDTLIRGKYPRDMLELIGFSSTAHPLKVTDLPSLEWNEYTYGTNLQHALQLAREL
                     LASQRGRNRQIIVITDGEPTAHITDGEVFFDYPPTRETFEATLKEVVRCTRDGITINT
                     FLLEQTPYMARFIEDLMRVNRGRVINASPNALGSYVLRDFVRGRTVQRFAS"
     gene            2185..>2797
                     /locus_tag="VFQ54_00415"
     CDS             2185..>2797
                     /locus_tag="VFQ54_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEU0163469.1"
                     /translation="MSIAEGQWREVALSDSEYAQILELLGREPSPVELGMFGAMWSEH
                     CGYKHSRPHLRKLPNDAPWVVQGPGENAGAVDIGNGLAAVFKVESHNHPSAVEPFEGA
                     ATGVGGIVRDIFTMGARPIAVLDGLRFGPLDDVRNRYLFDHVVAGIGGYGNCLGIPTV
                     GGEVFFDESYSGNPLVNAMCVGIAPIDGIVRAKASGIGNLVVLI"
BASE COUNT          583 a          879 c          869 g          466 t
ORIGIN      
        1 catcccaatg caccacttcc cgacgtcgta cacctaccac caatgggacg ggaccggaga
       61 cgaattcctc aatccggaag acatgatgga atccctctcc gaggatcttc tccatcacgg
      121 cgatatcgac ctcgcgctcc aacgcgcgtt ccgctgggga ttccagaacc gcgacggcca
      181 gcattccgac ggcctgcggg atctcctcaa tcggctgcgc cagcagcggc aggagatgct
      241 cgaccgctgg gatttcaact cgatcaccga ggacatccag gagcggctcg accagatcct
      301 cgaccaggag cggtcgacga tccaggagaa gaaggcacag ctcgaccggc aggcggcggt
      361 cgactctgca ctggacaccg acgccgcgaa gcagttcctc gaccgcaagg agacctcgct
      421 cgaggatctt ccggagagca ctgccgccca gatccagcag ttacgggaat acggattcgt
      481 cgatcagcag gcgcaggccg atttcgacaa gcttctccag gaactccagc agcaggtcgc
      541 cgacagcctc ttcaacaaca tgaagggagc gatgggcggc ggcgaagacg gctctggctc
      601 catggaagaa atgaaggagt tcctcaagga catcaatgat gccttcgagg ctcagcgcaa
      661 cggtgagcag gtcgatctcg acaagctgaa caaccggtgg gcgaatcaga tgggtggtcg
      721 catcgacagc ctcgaagagc ttgcgcagcg aatgcggcag cgtctcgcgg cggcccaggc
      781 gatgatgaac atgctgagcc cgcagcagcg cgccgatctg atgaagatga tgcagcaggc
      841 gatgcaggac gccggtctcg ccggcgaact cggccgcttg caggagaatc tcgggccgat
      901 tcgtgccccg cgtggcctcc agcaatccga cgctcatggc gccgagccac tctcgatcga
      961 catggcgacg agcatcctcc agcaagcgaa ccagatgggc cggctcgaga atcagctccg
     1021 gtcggtctcg cgcctcgagg aactcgacgg catcgatccc aagctcctcg acgaggtcct
     1081 caacgacgag gacaaggcgt ggctcgaccg ttggcagcat atcgagcagg aattgcagga
     1141 ctccggctac gtcaaccaga ccgatcgtgg cctcgaattg acgccacgtg cggtgcgcaa
     1201 gatcggcgag aaggcgctgg cggacatctt cggccagctc aagcagaacg ggatcggcga
     1261 tcacgatctg gacaaccggg gtgtcggcgg cgaattggcc gatacgacct cgcaatggga
     1321 gttcggcgat ccgttccagc tcgatctcgc gaaatcggtc atgaatgcga tcatgcggga
     1381 aggacccggc gcgcccgtcc acctgcgacc ggacgacttc gaagtcgtcg accgcgatgc
     1441 gaagacgtcg acagccaccg tgctcctcgt cgacatgagt cgctcgatga tccacaacgg
     1501 gtgctgggag gcggcgaaac gcgccgcgct cgccctcgat accctgatcc ggggaaagta
     1561 cccgcgcgac atgctcgagc tgatcggctt ctcctcgaca gcgcacccgc tgaaggtgac
     1621 cgatttgccg tcgctggagt ggaacgaata cacctacggc acgaatctcc agcacgcgct
     1681 gcaactggca cgtgaactcc tggcgagcca gcggggtcgc aaccgccaga tcatcgtgat
     1741 caccgacggc gaaccgacgg cgcacatcac ggacggcgaa gtcttcttcg actacccgcc
     1801 gacgcgggag accttcgagg cgacgttgaa ggaagtcgtt cgctgcaccc gggacggcat
     1861 cacgatcaac acctttctcc tggagcagac gccatacatg gcgcgcttca tcgaggatct
     1921 gatgcgggtg aatcggggtc gcgtcatcaa cgcatccccg aatgcactcg ggagctacgt
     1981 gctccgcgat ttcgtgcgag gacgcaccgt gcaacgattc gcttcctgaa cctgaggtgc
     2041 gaatcggcta aggaatggta agataccttt atcgtcgggc aggcgacacg agcgcctgcc
     2101 ccgaggacga ccatcgggcg atgtcgtcga tcattccccg ctcaaccccg cagtgtgaat
     2161 catcgagttc aaaaggagat ctccttgtcc atcgccgaag gtcagtggcg cgaagttgcc
     2221 ctgagcgata gcgagtatgc gcagattctg gagttgctgg gccgtgagcc gagcccggtc
     2281 gaactcggaa tgttcggcgc catgtggagc gagcattgcg gttacaagca ctcgcgaccc
     2341 catctccgca agctcccgaa tgacgccccc tgggtggtac agggaccagg cgagaacgcc
     2401 ggagccgtcg atatcggcaa cggactcgcg gcggtcttca aggtcgaatc ccacaatcat
     2461 ccgagtgcgg tggaaccctt cgagggtgcg gcaaccggcg tcggcggcat cgtgcgggac
     2521 atcttcacga tgggcgcacg tccgatcgcg gtactcgacg ggcttcgttt cggcccgctc
     2581 gatgacgttc gcaatcgcta cctcttcgat cacgtggtcg ccgggatcgg cggctacggg
     2641 aactgccttg gcattccgac cgtcggcggc gaggtcttct tcgacgagag ctacagcggc
     2701 aatccgctcg tcaatgcgat gtgcgtgggc atcgccccga tcgatggcat cgttcgcgcc
     2761 aaggcgagcg ggatcggcaa tctcgtcgtc ctgattg
//
LOCUS       DASTMD010000029         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_270565, whole genome shotgun sequence.
ACCESSION   DASTMD010000029 DASTMD010000000
VERSION     DASTMD010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270565"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VFQ54_00420"
     CDS             complement(<1..786)
                     /locus_tag="VFQ54_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0163470.1"
                     /translation="MPLDQQQRPFIDFVRSLRARDVTTYLSIGHKLGAAATPEIRELL
                     GDDVFASNTWLNADHYNDALHAAEALAADAWGADHTFYLVDGSSSGNHALFLAALRPG
                     DEVIVSRDLHWSMLVALIMTGAVPRYVAPRLHPELDIAFGIAAEDVAAALTSYPAAKL
                     VAIVSPSFCGVASDLEAIAAVAHARDVPLYVDEAWGAHFHFHPDLPASAMASGADAAV
                     ASVHKLLPALSQGSVLHVRGSRLDLHRINQTVHMTQTTSPLLPI"
     gene            complement(881..1876)
                     /locus_tag="VFQ54_00425"
     CDS             complement(881..1876)
                     /locus_tag="VFQ54_00425"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEU0163471.1"
                     /translation="MKSIGTMDTSDVSSEIASFPVFSPVDPGTIDAIRPWLAGVQHVA
                     DFNLVELWCWIRGRGDAGVSQLNGNLVVRWPAFPTHDTGEADLPGFVPDSGYLSVLPR
                     HQVERTILDLLAYTTSSTVNIQSSIRAIPLLDATLDNLSESGFVMTEDRDNFDYMISV
                     HDWARMAGGSFRNKRNVIHRLERATAIDWRISSPENREVAAGIPEVIARWADQRGRDR
                     SEVAQESTAIANLLDHAPSPGVFVVTAFADDVMIGFSINEVVDASYAVGHFAKADYRF
                     PGLSTAMLHRVCLHLAASGVRMLNIEADLGDPGLRRAKMLLRPSGFLRKVMVAPI"
     gene            2034..>2906
                     /locus_tag="VFQ54_00430"
     CDS             2034..>2906
                     /locus_tag="VFQ54_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163472.1"
                     /translation="MVAQSRISIGYLGISILAVASYFAMSGTPQLLTYDGIGIFAVAG
                     VIVGIWMYRPAAALIWNTIAFGLLLMITGDLIGNIADITGETLPFPSVSDGFYLVGNL
                     AIVLACAMVIRRRNTFRDIGGFIDATILATSATLLIWTVMIQPYTDDPSLSIPQRFVS
                     LAYPAMDVIMAGVGVRLLLTGGRQSPSFYLITAALGATLVGDITYAELTLRDAYHSGD
                     AIDLAFLLWYLLWGAAALHPSMTMVTDGVDDPILRTSGLRMTGLVGAVLVVPITAIYH
                     WWRNEPFEIPVFVTG"
BASE COUNT          559 a          909 c          905 g          533 t
ORIGIN      
        1 gatcgggagg agcgggctcg tcgtctgcgt catgtggacc gtctggttga tccggtggag
       61 atcgagccgc gatccccgga cgtgcaacac cgatccctgt gaaagggcgg gcaggagctt
      121 gtggacgctc gcgaccgccg cgtcggcgcc gctcgccatc gccgatgccg gaaggtccgg
      181 atggaaatga aagtgcgcgc cccacgcctc atcgacgtag agcggaacgt cgcgcgcgtg
      241 cgcgaccgcc gcgatcgctt cgagatcgga cgcgacgcca cagaaggagg ggctgacgat
      301 cgcgaccagt ttcgccgccg ggtacgacgt gagcgcagcg gcgacgtcct cggcggcgat
      361 cccgaaagcg atgtcgagtt cggggtggag gcgtggcgcg acatagcgcg gaaccgcccc
      421 ggtcatgatc agcgcgacga gcatcgacca gtgaagatcg cgcgagacga tgacctcgtc
      481 tccgggacgg agggcggcga gaaagagcgc atggttcccg ctcgagctcc cgtcgaccag
      541 gtagaaggtg tgatccgccc cccaggcatc ggcggcgagc gcctccgccg catgcagggc
      601 gtcgttgtag tgatccgcat tgagccaggt gttcgatgcg aagacgtcgt cccccagcag
      661 ttcgcgaatt tcgggcgtcg ccgcggcgcc aagcttgtgc ccgatcgaga gataggtcgt
      721 gacatcgcga gcgcgaaggg atcgaacgaa atcgatgaac ggccgttgct gctgatcgag
      781 gggcatggcg acgcggttac tccttcgcga atccacggat gggtctggca tgggatcagc
      841 ccgggattcc gacgactcgt gaggactggc aggactgcgg tcagatcggc gcgaccatca
      901 ccttccgcag gaacccggac ggtcgcagca gcatcttcgc gcgacgcagg ccgggatcgc
      961 ccaagtctgc ttcgatattg agcatccgga cgccggatgc ggcaagatgc aggcaaacgc
     1021 gatggagcat cgcggtcgaa agacccggaa agcgatagtc ggcctttgcg aagtgaccga
     1081 ccgcgtacga cgcatcgacg acttcgttga tcgaaaaccc gatcatgacg tcatcggcga
     1141 aggcagttac gacaaagact ccaggagacg gtgcatggtc gagcaggttg gcgatcgcgg
     1201 tcgattcctg cgcgacctcc gaccggtccc gtccccgctg atccgcccaa cgcgcgatga
     1261 cctccgggat tcccgccgcg acctcccgat tttcaggcga cgaaatgcgc caatcgatgg
     1321 ctgtggcacg ttcgagccga tgaatgacat ttcgtttgtt gcgaaaacta ccccccgcca
     1381 ttctcgccca gtcatgaact gatatcatat agtcaaagtt gtctctatcc tcggtcatga
     1441 caaaaccgga ttccgagagg ttgtcaagcg tcgcatcgag aagcggaatg gcacgaatgg
     1501 acgattgaat gttgacggtc gaagatgtgg tatatgcaag cagatcgagg atagtgcgct
     1561 caacctggtg gcgagggagc acggagaggt agccactatc cgggacgaag ccgggaagat
     1621 cggcctcacc ggtatcgtgg gtcgggaagg ccggccagcg caccacgaga ttcccgttga
     1681 gttgggaaac gcccgcgtcc ccccgacccc gaatccagca ccagagctcg acgagattga
     1741 agtccgcgac atgctggacc ccagcaagcc aggggcggat cgcatcaatc gtcccgggat
     1801 cgactgggga gaagaccggg aaggacgcga tctcgctgga aacgtcactc gtatccattg
     1861 ttccgataga tttcaccgct ccatcccggt tgaacgatcg acccaagtcc cggacgaacg
     1921 gcttggacat atcgactgaa tccacgctcc gcgcgcacca catcgcgggg tcccggcaaa
     1981 tactccgcta gacccacgac ggccgtcagc actggattcg aagggctcgg gcattggttg
     2041 cgcaatctcg catttcaatc ggctaccttg gaatctcgat cctggccgtc gctagctatt
     2101 ttgcgatgtc cggcacccct caactgctga cctacgacgg gatcggcatt ttcgccgtcg
     2161 ccggtgtcat cgtcggaatc tggatgtacc gaccggccgc ggcgctgatc tggaacacga
     2221 tcgcgttcgg actcctcctc atgattacgg gggatctgat cgggaacatc gccgatatca
     2281 ccggcgagac cttgccgttt ccatccgtca gtgacggatt ctatctcgtt ggtaacctcg
     2341 cgatcgtgct cgcctgcgcc atggtcatcc gtcgccgcaa cacgtttcgg gacatcggcg
     2401 gcttcatcga cgccacgatt cttgccacct cggcgacgct gctcatctgg acggtgatga
     2461 tacagcccta taccgatgac ccgtcgctgt cgatcccgca gcggttcgtc tccctcgcct
     2521 atcccgcgat ggacgtgatc atggccggcg ttggcgtgcg gcttctcctc accggaggac
     2581 gccagtcacc atcgttctac ctcatcaccg cggcacttgg ggccaccctc gttggcgaca
     2641 tcacctacgc cgagctgacg cttcgcgacg cctatcacag tggcgacgcc atcgatctcg
     2701 ccttcctgct ctggtatctc ctctgggggg cagccgcact ccatccttcg atgacgatgg
     2761 tcacggacgg tgtcgacgat cccatcctcc gcacgtccgg actgcgaatg accggtctcg
     2821 tgggggcggt gctcgtcgtt ccgatcacgg cgatctacca ctggtggcgg aacgagccgt
     2881 tcgagattcc cgtcttcgtg accggc
//
LOCUS       DASTMD010000030         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_538376, whole genome shotgun sequence.
ACCESSION   DASTMD010000030 DASTMD010000000
VERSION     DASTMD010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538376"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1125)
                     /gene="cofD"
                     /locus_tag="VFQ54_00435"
     CDS             complement(157..1125)
                     /gene="cofD"
                     /locus_tag="VFQ54_00435"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEU0163473.1"
                     /translation="MSLAHSGRRVTALAGGVGGAKLAQGLARVVPGENLTVIVNTADD
                     FELYGLNISPDLDTVMYTLAGIANPIFGWGIDGDTQATLGSMKRLGENTWFLLGDQDF
                     ATHILRTHRLRSGESLTAITADFARALGIGARMIPMTDQTVSTLVDTPAGRLGFQDYF
                     VARHQSDDVTGVVFDGIDDAAPAAGVIEAIGEADILVINPSNPIVSIGPILAVPGVRA
                     ALAESSAKRVGVSPIIGGKALKGPADKMLETLGHEVSALGIARIYAGLLDLLVIDEQD
                     RDLQGAIEALGIRVLITNSIMGDAEDRERLAREILAATEALTGVAR"
     gene            1305..>2503
                     /locus_tag="VFQ54_00440"
     CDS             1305..>2503
                     /locus_tag="VFQ54_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEU0163474.1"
                     /translation="MPNVSLVEAVAPLVIAIDVGSSSVRTLVYDAVGNQVSGTEARSS
                     HTLRADKDGASEADPDLLLDLLCRSLDRTLAESEARAGDLAAVGMSCFWHGLMGIDGE
                     GAPTTPIYMWADKRAGGMVAGLASDYEPQEAVRLTGCRMHSSYWPAKLRWLRATNGQA
                     FDSTSHWVSFPDYVSLKLTGSLRTSVSMASGTGLMSSETTGWFEPMIRHAKIEPAQLP
                     EIFDRDQPLPPLLPEFGERWPPLSTIPWFPALGDGAAANVGAGCVGPDRIALTVGTSG
                     AMRTIVPISEEHDRVPPGQLWVYRLDRHREVIGGALSNGGNVTAWLSETVSGRSMDDL
                     TREAESVAPDGHGLTILPFLAGERSPSWDDSATGVFAGLTLATAPAELFRAILEATAY
                     RFAAIHD"
BASE COUNT          438 a          832 c          800 g          433 t
ORIGIN      
        1 ggatcccctc ggttaccagc gagttcgcca gcgcatggag gacgtgccgt cccatttccg
       61 cgatcagcgc ggcacgatcc tcgggcgaga acgcgttcgc gagccgcgtc tttccggacg
      121 tgccgtcccg gagcgggacg acggcaagga ttccgttcac cgggcgacgc ctgtcaacgc
      181 ctcggtcgcg gcgagaatct cgcgtgccag gcgctcccga tcctccgcgt cacccataat
      241 cgaattggtg atgagaacgc ggatgccgag cgcctcgatc gcaccctgca ggtcgcgatc
      301 ctgctcgtcg atgacgagga gatcgagaag accggcatag atgcgagcga tcccgagcgc
      361 cgaaacctcg tgcccaagcg tctccagcat cttgtccgcc ggacccttca gcgccttgcc
      421 gccgatgatc ggactgacac cgacccgttt tgccgatgac tccgccagcg cggcgcggac
      481 gcccggtacg gcgagaatcg gaccgatgct gacgatcgga ttcgacgggt tgatgacgag
      541 gatatccgcc tcgccgatcg cctcgatgac acccgccgcg ggcgccgcgt cgtcgatgcc
      601 gtcgaagacg accccggtca cgtcatcaga ctgatgccgc gccacgaaat aatcctggaa
      661 gccgagacgt ccggcaggag tgtcgacaag cgtcgagacc gtctggtcag tcatcgggat
      721 cattcgcgcc ccgattccga gagcgcgggc gaagtcggcg gtgatggcgg tgagtgattc
      781 accgctgcgc agacgatgcg tgcgcaggat gtgcgtcgca aagtcctggt cgccgaggag
      841 gaaccaggta ttctccccca gccgcttcat ggagccgagc gtcgcctggg tatcgccgtc
      901 aataccccag ccgaagatgg gattggcgat tcccgccagt gtgtacatga ctgtgtcgag
      961 gtcgggactg atgttgaggc cgtagagttc gaaatcgtcg gcggtgttga cgatcaccgt
     1021 gaggttctcg ccggggacga cccgcgccaa cccttgcgcg agtttcgcgc cgccaacacc
     1081 cccagcaagg gccgttacac ggcggccgga atgtgccaaa ctcacgagca tatccttgat
     1141 ccaacgcgac cgacgccggt cgtcatcgct acacagtagc atacgattgg taaccatcca
     1201 actggtgaaa caggcggtat tgtcgcgaat tcgatgtcac acgcaggcgc tcccgctcgt
     1261 ccgccgcgag cggcgcatgc ggaaagcagg ccgagaggaa acccatgccg aacgtcagtc
     1321 tcgtcgaagc cgtcgccccg ctcgtgatcg cgatcgatgt tggatcgtca tcggtccgca
     1381 cgctcgtgta cgacgcggtg gggaatcagg tttccggcac cgaggcgaga tccagtcaca
     1441 cattgcgggc tgacaaggat ggtgcatccg aggccgatcc cgatctcctc ctcgatcttc
     1501 tatgcaggtc gctcgatcgc acgctggcgg agagcgaggc gcgagcgggt gatctcgcgg
     1561 cggtcgggat gtcctgcttc tggcacggcc tgatgggaat cgatggcgaa ggcgccccaa
     1621 cgacgccgat ctacatgtgg gcggacaagc gtgccggcgg catggttgcc ggtctcgcca
     1681 gcgactacga gccgcaggag gccgttcgac tcacgggttg tcgcatgcac tcgagctact
     1741 ggccggcgaa actgcggtgg cttcgcgcca ccaacggcca ggccttcgac agcacctccc
     1801 actgggtctc cttccccgac tacgtctcgc tcaagctgac aggatcgctc cgaacctctg
     1861 tctcgatggc gtctggtacc ggcttgatgt ccagcgagac gaccggctgg ttcgagccga
     1921 tgatccgcca cgcgaagatc gagccggcac aacttccgga aatcttcgac cgcgaccagc
     1981 cgctcccgcc gttgcttccc gaattcgggg agagatggcc cccactctcg acgattccat
     2041 ggtttcccgc gctcggggat ggagcggcgg cgaacgtggg cgccggctgc gtcggcccgg
     2101 accggatcgc gttgaccgtc ggcacctcgg gcgcgatgcg gacgatcgtg cccatttcgg
     2161 aggagcacga ccgcgttcct ccgggtcagc tctgggtcta ccgtctcgat cgccatcgcg
     2221 aggtgatcgg cggcgccctc agcaatggcg gcaacgtcac cgcctggctc tccgaaaccg
     2281 tatccgggcg gtcgatggat gaccttaccc gggaggcgga atcggtcgcc cccgacgggc
     2341 acggcctgac catcctcccg ttccttgccg gcgagcgatc cccgagctgg gacgacagtg
     2401 cgacgggtgt ctttgccggc ctcacgctcg ccacggctcc cgccgaactc ttccgcgcca
     2461 ttctcgaggc gacggcctac cggttcgcgg caatccacga tga
//
LOCUS       DASTMD010000031         9839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2770159, whole genome shotgun sequence.
ACCESSION   DASTMD010000031 DASTMD010000000
VERSION     DASTMD010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2770159"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VFQ54_00445"
     CDS             <1..672
                     /locus_tag="VFQ54_00445"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU0163475.1"
                     /translation="PVVIHDHTLERTTNGSGAVNGYTVAELQQFDAGQGEKIPTLAEV
                     FALVGDRIPFFLELKTLDAVPATVELIGRNPQIRWTALSGFPAALDALRVAFPDGDLR
                     LGTFGSRTAAEKLADRLKDSNYPISQAFLDRLRNDAASFDIETVFARAAEIRASKLGV
                     YHEGVDAELVEKVHARGLQIGAWTVNDPVEAKRLMEIGVDSVTTDDPAAILAMIAATR
                     ETPVA"
     gene            786..1808
                     /locus_tag="VFQ54_00450"
     CDS             786..1808
                     /locus_tag="VFQ54_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163476.1"
                     /translation="MQIPGFSKAGKFYKGNFHMHSTKSDGTLSPAETLEAYKSRGYDF
                     VSLTDHFLDRDSLMKLVGRTEYEGVGEFITVTDTTALRTDDFTTILGAEYHGTGMSNG
                     TPWHMVAVGLPLDFAPWDGKESIASITERAKNAGAFIGIAHPAASLVTMDDVKQVLPF
                     ADAIEVYNHAANLFSDGESWYFSDILLQGGNRLTTYAADDSHSGGAPTSVLYRDAPAY
                     TENSTPEERARNSDAFGGWVHVKAESLDPDALLAAIKAGDFYSSTGPEIKDIAIEDNE
                     IVVKTSPASGVRASGNGPTRFMKMAGAGPLIEEVRFPFTPEGYVRITVFGPDGTSAWS
                     NPIWFS"
     gene            1861..3600
                     /locus_tag="VFQ54_00455"
     CDS             1861..3600
                     /locus_tag="VFQ54_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0163477.1"
                     /translation="MDKNLDRWVKQTSISRRDFMKVLGVAGAAAGLTGTGAKAFASGT
                     SGEPRVLSVNQSNGDRPELVIGVTGLPPVLDPAQGSFFSIVGSRTTPIFHEPLLDIDY
                     KHGNPVGFGSNIIPALAEGWQQVDDTTLDVFLRDGILFQDGTPITADDVVYTINRIID
                     PNTDPKLKAVTTFFSTFKSVEAIDPKTVRVTTKQPDATLLDRLTVAYIVPKAVVEKDG
                     IANYALMPIGAGAYKVSEFVPNDHLTLESFDQFYGGTPPFSKVTIRVIVEVATRMAAF
                     SSNEIQLTTDVPPDQISQLQGDSNNVITSIGASNAHFLFFNTKQKVLSDVRMRQALTL
                     SIDRQLLVDTLWMGNADLMRGYQVPEWGSMYNPDRPFFTYDLDQAKKLLDEVDYDGTE
                     ISFRGPDGYYPLGNDAIEAIVGMWQDLGIKAKVDLLQPAAYTASFPTLMVGFISAGLN
                     PFDPEMNFSTLWDKNGTFQADFWTPEDPKFNQDLDLLTSTLDTKARFNAYQEALDIWE
                     KEVPATMLYRIHAIYGQKKSVGWTPYTVFNMDLRPANFGTAAPTGGATPTSGTPEAGE
                     ASPIASPEASPAS"
     gene            3784..4581
                     /locus_tag="VFQ54_00460"
     CDS             3784..4581
                     /locus_tag="VFQ54_00460"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU0163478.1"
                     /translation="MLIVAHRGGSNGTPEDTLAAFSNAVKIGADMIELDIHVSKDGVP
                     VVIHDHTLERTTNGTGDVNSYTVAELQQFDAGQGEKIPTLAEVFALIGDRIRFILELK
                     TIEAVPATVDLLRANPQVRWVGFSTHDEALDQLRVAFPEAELSFGTMGSREASIKLAD
                     VLGQANIAHYKSFAERLRQDAENFDIENVFARAAELPAVKIGFYYESIDAGMVDLVHT
                     RGYQIGAFTVNDVAEGKRLLALGVDSLTTDDPAAMIAMVAEAGVAIA"
     gene            4727..5683
                     /locus_tag="VFQ54_00465"
     CDS             4727..5683
                     /locus_tag="VFQ54_00465"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEU0163479.1"
                     /translation="MEIAAFSKPGSFYKGNIHLHSNHSDGRLAPADVVEEYRSRGYDF
                     VAITDHFLDRDYLMGLVGRAEYEGAGEFINVNDTSALRSENFTTIFGSEIHGTGMANG
                     TNWHIVAVGLPLDFAPWDGEESGAAIAERAQKAGAFVGIAHPAASSVTIEDAKQIIPF
                     ADSIEIYNHACNLFGMTDSWHFADILYTEGNRLSAYAADDAHTIGERDAFGGWVHVKA
                     ESLEPDALLKALKAGDFYSSTGPEITNVAIEGDEFIVQSSPATAILVTGMGGYRYKLG
                     SADAPISEARFPLEDIKKMPYARVTVIGPNQERAWSNPIWFD"
     gene            5808..7544
                     /locus_tag="VFQ54_00470"
     CDS             5808..7544
                     /locus_tag="VFQ54_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0163480.1"
                     /translation="MDKKLDRLINATGISRRDFMKALGIAGAVAGLAGTGERTMAAGT
                     SGQPQILAVNQTGDRPELVIGVTGLPATLDPGQGSFFSVVGSRLTPVFFDPLVDVDFR
                     DGTPEGFGTRIIPAIAEGWQQIDDTTLELKIREGVLFQDGTPLTADDVKYTFDRVLDP
                     KADPKLKQVFTFYNTIDHVDVIDPQTVRIVTKNPDAALMQRLTVAYIVPKAATEAAGL
                     ANFSLKPIGAGAYTVTEFVPNDHISFASFDQYYGGTPPFSKITVRMIVEVATRMAAFS
                     SGEVQLVTDVPPDQISQLQGDSNNQITSIGASNAHMFYFNTKQKVLSDVRMRQALNIS
                     IDRQLLVDTLWMGNADLMRSYQVPEWGDMYNPNRAFSTFDTNQAKQLLDDVKYDGTEI
                     TFLQSDGYYPLGNDAVEAIIGMWQDVGIKASANFVQPAAYTQNLDKFMVAFISAGLNP
                     ADPELTFSTLWSKTGFFQANFWTPEDPAFNQNLDFLATTLDVKARYDAYQTVLDTWEK
                     EVPGALLYRIHAIYGQKKTVGWTPYTVFNMDLRPSNFGKPAPVGGATPSAATPAAGEA
                     SPVASPEASPAS"
     gene            7762..8553
                     /locus_tag="VFQ54_00475"
     CDS             7762..8553
                     /locus_tag="VFQ54_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU0163481.1"
                     /translation="MLIIAHRGGATTTPENTIASFTNALALGADVIELDIHLSKDGVP
                     VVIHDHTLERTTNGTGEVNSYTVAELQQFDAGQGEKIPTLAEVFALVGDKVPFVLELK
                     ALDAIPATVELIKQNPHIRWTGLSGVPGGLEALREAFPDVALCTGTMGSREGTLALVD
                     RIEQSDYPITQEYLDRMRADAENFTIETAFDRAAEVNASMLIIYYEGASAENVAAAKA
                     RGLHTGAWTVNDVADARRMMEYGFDSLITDDPAAMIAMVAEARQT"
     gene            8608..9636
                     /locus_tag="VFQ54_00480"
     CDS             8608..9636
                     /locus_tag="VFQ54_00480"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEU0163482.1"
                     /translation="MDQFAAFSKPGNFYKGNIHLHSTNSDGRLTPTEVIDAYKSRGYD
                     FVSLTDHFLPRAYFKKPGGGFITVTDTTNLRSDDFTTILGAEIHGPGQSNGTNWHIVA
                     VGLPADFAEWDGVETGAAIAERALNAGAFVSMAHPAASGLTIADADQVLPFIHSVEVY
                     NHGSKIVGAPESWHYTDTLLRNGARFTANATDDAHLVTAGGLIQDAFGGWIQVRAESL
                     DPDLLLAALKAGEFYSSMGPEIHDVAVEGDDLVIRCSPVLSVLIAGKGGFQWKFADGK
                     KIHDVWIQDAEVDVAADEAAAPSNGEEVLITEVRFPYAQIQKMEYGRITIVDRDGKYA
                     WSNPFWFD"
BASE COUNT         1823 a         3421 c         2887 g         1708 t
ORIGIN      
        1 cccgtcgtca tccacgatca caccctcgag cgcacgacga acggctcagg cgccgtcaac
       61 ggctacaccg tcgccgagct tcagcagttc gacgccggcc agggcgagaa gatcccgacc
      121 ctcgccgagg tcttcgcgct cgtcggtgac aggatcccgt tcttcctcga actgaagacg
      181 ctcgatgcgg taccggccac ggttgagctg atcgggcgca accctcagat ccggtggacg
      241 gcgttgagcg gtttcccggc ggcgctcgat gcgctgcggg tggccttccc ggatggcgat
      301 ctccgcctcg gcaccttcgg ctcgcgcacg gcagccgaga agctcgccga tcgcctcaag
      361 gattcgaact acccgatctc ccaggcgttc ctcgatcgcc tgcgcaacga cgcggcttcc
      421 ttcgacatcg agaccgtctt cgcgcgtgcc gccgagattc gcgcctcgaa gctcggtgtc
      481 tatcacgagg gcgtcgacgc cgagctggtc gagaaggtcc acgcgcgcgg tcttcagatc
      541 ggtgcgtgga cggtcaacga tccggtcgag gcgaagcgcc tgatggagat cggcgtcgat
      601 tccgtcacca ccgacgatcc ggccgcgatt ctcgcgatga tcgccgccac ccgggagacc
      661 ccggtggcct gacacgcccg gcgggacatt gccgcccgcc ccgattccgg cgatgacgcc
      721 ggaacgttcg acgacgagcc ctcgcatccg gcaattcggt tcacccgttc acgcaggagt
      781 acatcgtgca gatcccaggg ttcagcaaag cgggcaagtt ctacaagggc aacttccata
      841 tgcattcgac gaagtcggat ggcacccttt ccccggccga gacgctcgag gcctacaagt
      901 cccgcggcta cgatttcgtc tcccttaccg atcacttcct cgatcgtgat tccctcatga
      961 agctcgttgg ccgcacggag tatgagggcg tcggtgagtt catcaccgtc accgatacga
     1021 ccgcgcttcg caccgacgat ttcacgacga tcctcggcgc cgagtaccac ggaaccggca
     1081 tgagcaacgg cacgccgtgg cacatggtcg ccgtcggcct gccgctcgac ttcgcgccgt
     1141 gggatggcaa ggaatcgatc gcctcgatca ccgagcgcgc gaagaatgcc ggcgccttca
     1201 tcggtatcgc ccacccggcg gcttccctcg tcacgatgga cgacgtgaag caggtgctcc
     1261 cgttcgccga tgcgatcgag gtctacaacc acgccgccaa cctcttcagt gacggtgaga
     1321 gctggtattt cagcgacatt ctcctccagg gcggcaatcg cctgaccaca tacgccgcgg
     1381 acgattccca ctccggtggc gccccaacct cagtcctcta ccgcgacgct cctgcctaca
     1441 ccgagaactc caccccggaa gaacgtgcgc gcaacagcga cgccttcggt ggctgggttc
     1501 acgtcaaggc cgagtcactc gacccggacg ccctgcttgc cgcgatcaag gccggcgact
     1561 tctattcctc caccggaccc gagatcaagg acatcgcgat cgaggacaac gagatcgtcg
     1621 tcaagacctc ccctgcatcc ggcgttcgcg ccagtggcaa cggcccgacc cggttcatga
     1681 agatggccgg cgccggtccg ctgatcgagg aagtccgctt cccgtttaca ccggaaggct
     1741 acgtccgcat caccgtcttc ggacccgacg ggaccagcgc ctggtccaat ccgatctggt
     1801 ttagctaagc accgttcgct ctaccgttcc gtttctcttc attcacgtca ggagtctctc
     1861 gtggacaaga atctcgatcg atgggtcaag cagactagta tctcgcgtcg tgacttcatg
     1921 aaggtgctcg gcgtcgccgg ggccgccgcc ggtctcaccg gcacgggcgc caaggcgttc
     1981 gcttccggca cgtccggtga gccgcgcgtc ctctccgtca accagagcaa cggcgatcgc
     2041 cccgagctcg tgatcggcgt caccggcctc ccgccggtcc tcgatcccgc ccagggcagc
     2101 ttcttctcga tcgtcggctc ccgcacgacg ccgatcttcc acgagccgct gctcgatatc
     2161 gactacaagc acggcaatcc ggtcgggttc ggctccaaca tcattccggc gctcgccgag
     2221 ggctggcagc aggtcgatga cacgaccctc gatgtcttcc tccgcgacgg catcctcttc
     2281 caggacggca cgccgatcac ggctgatgat gtcgtctaca cgatcaaccg catcatcgac
     2341 ccgaacaccg atccgaagct gaaggccgtg acgaccttct tctcgacctt caagagcgtc
     2401 gaggcgatcg atccgaagac ggttcgcgtc acgaccaagc agccggacgc cacgctcctc
     2461 gatcgcctca ccgtcgccta catcgtgccg aaggccgttg tcgagaagga cggcatcgcc
     2521 aactacgccc tgatgccgat cggcgccggc gcctacaagg tctccgagtt cgtgccgaac
     2581 gaccacctga cgctcgagtc cttcgatcag ttctacggtg gcacgccgcc gttctcgaag
     2641 gtgacgatcc gcgtcatcgt cgaagtcgcg acccgcatgg ccgccttctc cagcaacgag
     2701 atccagctca cgaccgacgt tccgccggac cagatctccc agctccaggg tgacagcaac
     2761 aacgtcatca cctcgatcgg cgccagcaac gcgcacttcc tcttcttcaa cacgaagcag
     2821 aaggtgctgt ccgacgtccg catgcgccag gcactgaccc tctcgatcga tcgccagctg
     2881 ctggtcgaca ccctctggat gggcaacgcc gacctcatgc gcggctacca ggtgccggag
     2941 tggggttcca tgtacaaccc cgaccgcccc ttcttcacgt atgacctcga ccaggccaag
     3001 aagctcctcg atgaggtcga ctacgacggc accgagatca gcttccgtgg tccggacggc
     3061 tattacccgc tcggcaacga cgcgatcgag gcgatcgtgg gcatgtggca ggacctcgga
     3121 atcaaggcga aggtggacct gctccagccg gcggcctaca ccgccagttt cccgacgctg
     3181 atggtgggct tcatctcggc cggtctcaat ccgttcgatc cggagatgaa cttctccacg
     3241 ttgtgggaca agaacggcac cttccaggcg gacttctgga cgccggagga tccgaagttc
     3301 aaccaggatc tcgacctcct cacctccacg ctcgacacga aggcccgctt caacgcctac
     3361 caggaggcgc tcgacatctg ggagaaggaa gtcccggcga cgatgctgta tcgcatccac
     3421 gccatctacg gtcagaagaa gtcggtcggt tggacgccgt acacggtctt caacatggac
     3481 ctccgcccgg cgaacttcgg cacggcggcg ccgaccggtg gcgcgacccc gacatccggc
     3541 acaccggaag ccggtgaggc ttcgccaatc gcctccccgg aggccagccc ggcctcctag
     3601 gcgtcccatt cggggcgaca tagaagtcgc cccgtactca cgaccgctgt acgtacggcg
     3661 gtcatgagta cggcgggact tctatgtccc gcctccgctc gcgggcggcc agcctcgccg
     3721 cagcgcgata tgaagctggc aatcaaggcg ccaagccata ccggttcaga aaggttcttc
     3781 gtcatgttga tcgttgccca ccgcggtgga tcgaacggaa ccccggaaga tacgctcgct
     3841 gccttctcga acgccgtgaa gatcggcgcc gacatgatcg aactcgacat ccacgtttcg
     3901 aaggatggcg tgcccgtcgt gatccacgat cacaccctcg agcgcacgac gaacggcacc
     3961 ggcgacgtca acagctacac cgtcgccgag cttcagcagt tcgatgccgg ccagggtgag
     4021 aagatcccga ccctcgccga ggtcttcgcc ctcatcggcg acaggatccg cttcatcctc
     4081 gagttgaaga ccatcgaagc ggttccggcg acggtcgatc tgctccgcgc caatccccag
     4141 gtccgctggg ttggcttcag cacccacgac gaggcactcg accagctgcg cgtcgccttc
     4201 ccggaggccg aactctcctt cggcacgatg ggttcgcgcg aggcctcgat caagcttgcc
     4261 gacgtcctgg gccaggccaa catcgcgcac tacaagtcgt tcgccgagcg cctccgccag
     4321 gatgccgaga acttcgatat cgagaatgtc ttcgcacgcg ccgccgaact cccagccgtc
     4381 aagatcggct tctactacga gtcgatcgat gccgggatgg tcgatctcgt tcacacccgc
     4441 ggctaccaga tcggcgcctt cacggtgaat gacgtcgcgg aaggcaagcg cctgctcgcg
     4501 ctcggggtcg attcgctgac caccgatgac ccggccgcga tgatcgcaat ggtcgccgag
     4561 gctggcgtcg cgatcgccta gatccgcagg tcaaggctcg ggccgtgtgg ccccacatgc
     4621 atcactccgg accacgtcgg ccaagcgttc gttcgtaggg cgggacttct atgtcccgcc
     4681 tccccggacg agagcctatc tctcaccgca acaagggcat gcacacatgg aaatcgccgc
     4741 gttcagcaag ccgggttcct tctacaaggg caacattcac ctgcactcga atcactccga
     4801 tggtcgcctc gcgccggcgg atgtggtcga ggagtaccgc tcccgcggct acgacttcgt
     4861 cgccatcacc gatcacttcc tggatcgcga ctacctgatg ggtctcgtcg gtcgcgccga
     4921 gtacgaaggc gccggcgagt tcatcaacgt caatgacacc tcggcgctgc gctccgagaa
     4981 cttcacgacg atcttcggct cggaaatcca cggcaccggc atggcgaacg gcaccaactg
     5041 gcacatcgtc gccgtcggcc tgccgctcga tttcgcgccg tgggacggcg aggagtccgg
     5101 cgctgcgatc gccgagcgcg cccagaaggc gggcgccttc gtcggcatcg cgcacccggc
     5161 ggcatcgtcg gtcacgatcg aggatgcgaa gcagatcatc cccttcgccg attcgatcga
     5221 gatctacaac cacgcctgca acctcttcgg catgaccgat agctggcact tcgccgacat
     5281 cctctacacc gaaggcaacc gtctctccgc ctacgccgcg gacgatgccc acacgatcgg
     5341 cgagcgcgat gcgttcggcg gctgggttca cgtcaaggcg gaatcgttgg agcccgatgc
     5401 cctgctcaaa gcgctgaagg ccggcgattt ctactcctcc accggacccg agatcaccaa
     5461 cgtcgcgatc gagggggacg agttcatcgt ccagtcctcc ccggccacgg cgatcctcgt
     5521 caccggcatg ggcggttatc gctacaaact cggcagtgcc gatgctccga tctcggaagc
     5581 ccgcttcccg ctcgaggaca tcaagaagat gccctacgcc cgcgtgaccg tcatcggtcc
     5641 gaaccaggag cgcgcctggt cgaatccgat ctggttcgac taggcgtctc cgccgggcgg
     5701 cggcatgctg ctgccgcttc aaccacgaat ccggtgggtc ccagggcgac gcaatggtgc
     5761 gattcgtcca cgtcacattc cgtcagcagc cagttcagga gcattcgatg gacaagaaac
     5821 tcgatcgtct tatcaacgcg accggaatct cccggcgtga cttcatgaag gcactcggca
     5881 tcgccggcgc ggtcgcgggt ctcgccggta ccggcgagcg cacgatggcg gccggaacct
     5941 ccggtcagcc gcagatcctc gccgtcaacc agaccgggga tcgcccggaa ctcgtgatcg
     6001 gcgtcaccgg tctcccggcc acgctcgatc cggggcaggg cagcttcttc tcggtcgtcg
     6061 gttcgcgcct cacaccggtc ttcttcgacc cgctggtgga tgtcgatttt cgcgacggca
     6121 cgccggaagg cttcggcacc aggatcatcc cggcgatcgc cgagggctgg cagcagatcg
     6181 atgacacgac gttggagctg aagatccgcg agggtgtgct cttccaggat ggcacgccgc
     6241 tgaccgcaga cgacgtgaag tacacgttcg accgcgtcct cgatccgaag gccgatccga
     6301 agctgaagca ggtcttcacc ttctacaaca cgatcgatca cgtcgacgtg atcgatccgc
     6361 agaccgttcg cattgtgacc aagaacccgg acgccgcgct gatgcaacgg ctcacggtcg
     6421 cctacatcgt gccgaaggcc gcgaccgagg cggcgggact cgccaacttc tcgctgaagc
     6481 cgatcggcgc cggcgcctac acggtgaccg agttcgtgcc gaacgatcac atcagcttcg
     6541 cgtcgttcga tcagtactac ggcggcacgc cgcccttctc caaaatcacg gttcgcatga
     6601 tcgtcgaggt cgcgacccgc atggccgcct tctcgagcgg cgaagtgcag cttgtgacgg
     6661 acgttccgcc ggatcagatc tcgcagctcc agggtgacag caacaaccag atcacctcga
     6721 tcggcgccag caacgcgcac atgttctact tcaacaccaa gcagaaggtc ctttcggacg
     6781 ttcgcatgcg ccaggcgctc aacatctcga tcgaccgcca gctgctggtc gataccctct
     6841 ggatgggcaa cgccgacctg atgcggagct atcaggtgcc ggagtggggc gacatgtaca
     6901 accccaaccg cgcgttctcg accttcgaca ccaaccaggc gaagcagctc ctcgatgacg
     6961 tcaagtacga cggcaccgag atcaccttcc tccagtccga tggctactac ccgctcggca
     7021 acgacgcggt cgaggcgatc atcgggatgt ggcaggacgt cgggatcaag gcgagcgcca
     7081 atttcgtgca gccggcagcc tatacccaga atctcgacaa gttcatggtc gccttcattt
     7141 ccgccggtct gaaccccgcc gatccggagc tgaccttctc cacgctctgg tcgaagacgg
     7201 gcttcttcca ggccaacttc tggacgccgg aagacccggc cttcaaccag aatctcgact
     7261 tcctcgccac gacgctggat gtgaaggcgc gctacgacgc ctaccagacc gtcctcgaca
     7321 cctgggagaa ggaagttccg ggagcactgc tctatcgcat ccacgccatc tacggccaga
     7381 agaagaccgt tggctggacg ccatacaccg tcttcaacat ggacctccgc ccgtccaact
     7441 tcggcaagcc ggccccggtc ggtggcgcga cgccgtcggc ggccacgccg gctgctggtg
     7501 aggcatcgcc ggtcgcctcc ccggaggcca gccccgcgtc gtaggtgtca ctccggaggg
     7561 gcctcccggc ccctccggag gcgggacata aaagtcccgc cgtacgaacg accgccgcac
     7621 caacggggtt gatgaccgga acgcggcccc ccgaatgacg ttggggagaa ccggggtttg
     7681 tagggcgaga gttcatctct cgccgcccgc aagcggatcg cacgcagaac gtgccgccgc
     7741 aagaccagaa aggtcgttca catgctgatc atcgcgcacc ggggtggcgc cacaaccacg
     7801 ccggagaaca ccatcgcctc gttcaccaat gccctcgcgc tcggcgccga tgtcatcgag
     7861 ctcgatatcc atctctcgaa ggacggcgtc cccgtcgtca tccacgatca caccctcgag
     7921 cgcaccacca acggcacggg cgaggtcaac agctataccg tcgccgaact ccagcagttc
     7981 gacgccggtc agggtgagaa gatcccgacc ctcgccgaag tcttcgccct cgtcggcgac
     8041 aaggtgccgt tcgtcctcga gctcaaggcc ctcgacgcca tcccggccac ggtcgagctg
     8101 atcaagcaga accctcacat ccgctggacc ggtctgagtg gcgttccggg tggcctcgag
     8161 gcgctccggg aggccttccc ggatgtcgcg ctctgcaccg gcacgatggg atcgcgcgag
     8221 ggaacgctgg cgctggtcga tcgcatcgag cagtccgact acccgatcac gcaggagtat
     8281 ctcgaccgca tgcgcgcgga cgccgagaac ttcacgatcg agaccgcgtt cgatcgcgcc
     8341 gccgaagtca acgcctcgat gctgatcatc tactacgagg gagccagcgc cgagaacgtc
     8401 gccgcggcga aggcacgcgg tctccacacc ggcgcgtgga ccgtcaacga cgtggctgat
     8461 gcccggcgga tgatggaata cggcttcgat tccctcatca ccgacgaccc cgccgcgatg
     8521 atcgccatgg tcgccgaggc ccgccagacg taatccgcct gacccctgac ccgttcgctt
     8581 cttccataga caaggatttc tcccctgatg gaccagttcg cagcattcag caagcccggc
     8641 aacttctaca agggcaacat ccacctgcac tccaccaact ccgacggtcg cctcacgccg
     8701 accgaggtga tcgacgccta caagtcgcgt ggctacgact tcgtctcgct gacggatcat
     8761 ttcctgcccc gcgcctattt caagaagccg ggcggtggct tcatcaccgt taccgatacg
     8821 accaatctcc gctccgatga cttcacgacg atcctcggtg ccgagatcca cggaccgggt
     8881 cagagcaacg gcaccaactg gcacatcgtg gcggttggcc tcccggccga ctttgccgag
     8941 tgggacggtg tcgagaccgg cgccgcgatc gccgagcgcg cgctcaacgc gggcgccttc
     9001 gtgagcatgg ctcacccggc ggcttccggt ctcacgatcg ccgatgcgga tcaggtcctg
     9061 ccgttcattc actccgtcga ggtctacaac cacggatcga agatcgtcgg cgcgccggag
     9121 agctggcact acaccgatac cctgctgcgc aatggcgctc gtttcaccgc caacgcgacg
     9181 gacgatgccc atctcgtgac ggcgggcggc ctgatccagg atgcgttcgg cggctggatc
     9241 caggtcaggg cggaatcgct ggatccggat ctgctgctcg cggcgctgaa ggcgggtgag
     9301 ttctactcgt ccatgggacc cgagattcat gatgtcgccg tcgagggtga cgatctcgtc
     9361 atccgttgct cgccggtgct ctcggtgctg atcgccggga agggcggctt ccagtggaag
     9421 ttcgccgatg gcaagaagat ccacgatgtc tggattcagg acgcggaggt tgacgttgcg
     9481 gcggatgagg ccgctgctcc gagcaatggc gaagaggtcc tgatcaccga ggtgcgtttc
     9541 ccctacgccc agatccagaa gatggaatac ggacgcatca cgatcgtcga tcgcgatggc
     9601 aagtacgcgt ggtcgaaccc gttctggttc gactagccgg cgtttttcac ccaccgcata
     9661 aagcgcgacg aatggatcgg ggcggtcatg atgaccgccc cgattgtcat gaccggcgtc
     9721 gagggcgacg cgttgccatt cccgtcccgg cgacccatgt ggtcgccccc ctcacggggc
     9781 cgacagatgc cgtgccctga cgatctcgta gggggcgatc ctgatgatcg tctccggat
//
LOCUS       DASTMD010000032         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2412843, whole genome shotgun sequence.
ACCESSION   DASTMD010000032 DASTMD010000000
VERSION     DASTMD010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2412843"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFQ54_00485"
     CDS             complement(<1..435)
                     /locus_tag="VFQ54_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163483.1"
                     /translation="MTNFEWFLLVVVVIAVPLVVAVVVTLWTLEMARQRNRRNRPGAA
                     AAIGVKRNATRGATIAGDSFTASTAPVVGVVHGSTFSDPTAVNTDVPGEGSFSVDRGP
                     EVTPVPSPDATPDVSTPASDIDPGSSSSDGGSSGGDSGGSSSS"
     gene            complement(597..1943)
                     /locus_tag="VFQ54_00490"
     CDS             complement(597..1943)
                     /locus_tag="VFQ54_00490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU0163484.1"
                     /translation="MATVIMPKMGDAMEEGTLLKWLKSVGEEVAEGDPIAEIETDKVT
                     LELEAGEAGYLTSTLVEEGAVVPIGAPVATIGAESEIGQAAPAAAAPVAEAAAPAPAP
                     VPEPVAVAAPAAAAPAPAIVEAPAIAAAAQVVSRAPGERLRASPLVKRLAEEHGIDLN
                     QVAGTGPGGRIVKNDISPFLTGAQPAPKAAPAPVVIPAANGATAPVAASPAPATSGAT
                     PKGRKIELARIRRTTGKRMAESKAGIPHYYVTINVDMTDAIAFRKQINAAVGEDGVRV
                     SVNDLIVKAAALALRENPNVNTSFENGDLYQHDSIDINIAISLDGGLIAPFVPAADQK
                     SLGTIARMTKDLGNRARNGGLTPEEYQGGTFTISNLGMFDVDEFIAVINPPQAAILAV
                     GSAQEVPVVRDGEIAVGSVMKLTLSADHRALDGAEAARFLGSVKKFLQNPMLLAIS"
     gene            complement(1956..>2308)
                     /locus_tag="VFQ54_00495"
     CDS             complement(1956..>2308)
                     /locus_tag="VFQ54_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0163485.1"
                     /translation="GRQVNLLLRVAEKLEADKISAEVIDLRTIRPFDGDTIVASVRKT
                     HRAVVVQEQWRWFSVASEVAAIIQEQAFDELDAPVARVSGAEVPAPYSRSLEVAAFPS
                     EQHVIDAVKRTLYI"
BASE COUNT          381 a          806 c          736 g          385 t
ORIGIN      
        1 cgagctgctg cttccgccgc tgtctccgcc cgacgatccg ccatcgctcg aactcgatcc
       61 cggatcgatg tcgctcgccg gcgtcgacac gtccggcgtg gcgtctggtg acggaaccgg
      121 ggtcacctcg ggcccacgat cgacagaaaa acttccctca cccggcacat ccgtattgac
      181 ggccgtgggg tcggaaaatg tcgacccgtg gacgaccccg accaccggcg cggtcgacgc
      241 cgtgaaggaa tcaccggcga tggtcgcacc ccgggtggcg ttgcgcttca ccccgatcgc
      301 cgccgccgcg cccggacgat tgcgccggtt gcgttgcctc gccatctcga gcgtccacag
      361 cgtcaccacc acggccacga caagcgggac cgcgatcacc acgacgacga gcaggaacca
      421 ttcgaagttc gtcatgccac cctcactccg acatcgacca cgacgcttgc cgcgccgccg
      481 ccggactgac gctcacggac cgcgcccgaa agcacgatag taccgcaact cgcacgaagg
      541 ccggtcggaa cgatccgacc ggcccctgca tcatccagac cccttcgcca acgatcctag
      601 gagatcgcca gcagcatcgg gttctggagg aacttcttga cggaaccgag gaaacgagcg
      661 gcctcggcgc catcgagcgc gcggtggtcg gcagagagcg tcagcttcat cacgctgccg
      721 acggcgatct cgccgtcacg cacgaccggg acttcctgcg ccgagccaac cgcgaggatc
      781 gcggcctgcg gcgggttgat cacggcgatg aactcatcga catcgaacat cccgaggttg
      841 ctgatcgtga aggtgccgcc ctgatattcc tctggtgtga ggccaccatt gcgggcgcgg
      901 ttgccgagat ccttcgtcat tcgggcgatc gtgccgaggg acttctgatc ggcagccgga
      961 acgaacggcg cgatcaagcc gccgtcgagc gagatcgcga tgttgatgtc gatcgagtcg
     1021 tgctggtaaa gatcgccatt ttcgaacgac gtgttgacgt tcgggttctc gcgaagggcg
     1081 agggcggcgg cctttacgat aagatcgtta acggagacac gaacgccatc ctcgccgacc
     1141 gcggcattga tttgcttgcg gaacgcgatg gcatcggtca tgtcgacatt tatcgtgaca
     1201 taataatgcg ggatgccggc cttcgactcg gccatgcgct tgccggtcgt ccggcgaatg
     1261 cgggcgagct cgatcttgcg gcccttcggc gtcgcaccgg acgtcgcggg agcaggggag
     1321 gccgcaaccg gcgcggtcgc gccattggcg gcggggatca caaccggggc aggcgccgcc
     1381 ttcggcgctg gctgcgcgcc ggtcaggaac ggcgagatgt cgttcttcac gatgcgaccg
     1441 ccgggacccg ttcccgcgac ctggttgaga tcgatcccgt gctcctcggc aaggcgcttc
     1501 acgagcgggg atgcccgcag gcgctcgccc ggcgcgcggc tcacgacctg ggctgccgcg
     1561 gcgatggccg gggcctccac gatcgcgggg gcaggggccg cggcagcagg cgccgccacc
     1621 gcgaccggct cgggcacagg agccggggcg ggcgccgccg cctcagcgac cggggccgcg
     1681 gcggcagggg cggcctggcc gatctccgac tcagcgccga tcgtcgcgac cggggcgccg
     1741 atcggcacca cggcgccttc ctcgaccagc gtgctcgtga ggtagccagc ttccccggct
     1801 tcgagctcga gcgtcacctt gtcggtctcg atctcggcga tcggatcacc ttccgcgacc
     1861 tcctcgccga cactcttcag ccacttgagg agggttccct cctccatggc gtcgcccatc
     1921 ttgggcatga ttaccgttgc catgaggttt ccttactaga tgtacaacgt ccgcttcacg
     1981 gcgtcgatca catgctgctc gctggggaat gccgccactt ccagactccg ggaatacggc
     2041 gccggaactt ccgccccact gacccgtgcc accggcgcat cgagttcatc gaacgcctgc
     2101 tcctggatga tcgccgccac ttcgctcgcg acgctgaacc agcgccactg ctcctgcacg
     2161 acgaccgcgc gatgcgtctt ccgcaccgac gctacgatcg tgtcgccatc gaaggggcgg
     2221 atcgttcgca gatcgatcac ttctgccgag atcttatcgg cttccagctt ctccgcaacc
     2281 cggaggagga ggttcacctg gcgaccgt
//
LOCUS       DASTMD010000033         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1609742, whole genome shotgun sequence.
ACCESSION   DASTMD010000033 DASTMD010000000
VERSION     DASTMD010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609742"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(422..1258)
                     /locus_tag="VFQ54_00500"
     CDS             complement(422..1258)
                     /locus_tag="VFQ54_00500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU0163486.1"
                     /translation="MRFAFKTAPQHTTWDDMLTLWKEADQIELFESGWNFDHFYPIMG
                     DSHGPCLEGWITLTALAQATTRLRMGTLVTGIHYRHPAVLAKMVTSLDVISNGRVELG
                     IGAGWNEEESGAYGIPLGTKKERSDRFEEACEVLKSLLTNETTTFDGKYYQLNEARNE
                     PKGPQTPIPICIGGSGEQRTLRTTAKYADHWNYGGGSPEEFAHKRDVLYGHCEAIGRD
                     PKEIMLSSHLTFTTVDEVNKTAEQAAALKDVGLDLGIVYLRQPLDASVLEPIAKALEQ
                     VA"
     gene            complement(1400..1822)
                     /locus_tag="VFQ54_00505"
     CDS             complement(1400..1822)
                     /locus_tag="VFQ54_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163487.1"
                     /translation="MPMIDVYATNGTFANKRELTLALSEAVMKWEQVPPIALFRENTA
                     AFIHEMDPDAIGNAAGESNYVRVQVLTPAGVLDRDKQLGVVRELTDIVAAAAGDASLV
                     ERTWVLLHESPDGGWGVDGHANTNAEIAAAARKELSDH"
     gene            1917..>2137
                     /locus_tag="VFQ54_00510"
     CDS             1917..>2137
                     /locus_tag="VFQ54_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0163488.1"
                     /translation="MSSDSRQKMIASAASLIASRGVNATSFSDVLADSGAPRGSIYHH
                     FPEGKRQLAEDAIRWTSSRVLAFMRAGTPT"
BASE COUNT          379 a          693 c          646 g          419 t
ORIGIN      
        1 tgccgtgccg cgctttcttc ccgccgcgcg ggatagccac gaccggcccc accgttcgtc
       61 accagctctg gggcaacgcg catcgcctgc gaaatcccac aggcccggat ggctacgctg
      121 ccggcaagcg gggacaagcc ccgcggggac gggtacacga gattgcgcaa gcacctgctg
      181 cgcgacgcag cgacgcgccg tactcaccga cgccgcaccc aatgcggtcg tgcgtagggt
      241 gggactgcgg aatccaaaga ccgggggaca acacgtcttg ccaagcgata tccggtcttt
      301 ggatggagta atgtcccacc tcccctcccc acacagcatg tcttgggaac ggaaatgcgc
      361 gagcccggaa gtcggtcgac ttccgggctc gcgcatactt gccttctgat cggttgcccc
      421 gttacgcgac ctgctccagc gccttcgcaa tcggctcgag aaccgacgcg tccagcggct
      481 ggcgaagata gacgatcccc aggtcgagac cgacgtcctt cagcgccgcc gcctgctcgg
      541 ccgtcttgtt cacttcatcg acagtcgtga acgtcagatg gctggagagc atgatctcct
      601 ttggatcgcg gccgatcgcc tcgcagtgtc cgtagaggac atcgcgcttg tgggcgaact
      661 cctcgggcga accgccgccg tagttccagt ggtcggcata cttcgccgtc gtgcgcagcg
      721 tacgctgctc gccgctacca ccgatgcaga tcggaatcgg cgtctgcggg cccttcggct
      781 cgttgcgcgc ttcgttcagc tggtagtact tgccgtcgaa ggtcgtcgtc tcgttcgtca
      841 gcaggctctt gagaacctcg cacgcctctt cgaagcgatc gctccgctcc ttcttcgtgc
      901 cgagcggaat accgtaggca ccggactctt cctcgttcca gccggcgccg atgccgagtt
      961 cgacgcgacc gttcgaaatc acgtcgagcg aggtcaccat cttcgcgaga acggccggat
     1021 ggcgatagtg gatgccggtg acgagcgtgc ccatgcggag gcgggtcgta gcctgggcaa
     1081 gcgccgtaag cgtgatccag ccttcgaggc agggaccgtg cgaatcgccc atgatcgggt
     1141 agaagtgatc gaagttccat ccagattcga agagttcgat ctggtctgcc tctttccaga
     1201 gggtgagcat gtcgtcccag gtggtgtgtt gcggggcggt cttgaacgcg aagcgcataa
     1261 acgggctcct ctgatgacac cggctctgcc ggtccctctg ccgatggctc ctgatagaag
     1321 ccgtgcagga cgtctgacgc cctcgcggta cgtatatgac cgtttcagga ccttggcaag
     1381 accgcccgct tcacattcct cagtggtccg aaagctcttt acgcgcggcg gcggcgatct
     1441 cggcattcgt gtttgcgtgg ccgtcgacgc cccagcctcc atccggcgac tcgtgaagca
     1501 gcacccacgt ccgttcaaca agactcgcgt cacccgcagc ggcggccacg atatccgtca
     1561 gctcgcggac gacaccgagc tgcttgtcgc gatcgagcac accggccggc gtcaggacct
     1621 gaacccggac atagttgctc tcgcctgccg cgttgccgat cgcgtcggga tccatctcgt
     1681 ggatgaaggc ggcagtgttt tccctgaaga gcgcgatcgg cgggacctgc tcccacttca
     1741 tcacggcctc gctcaacgcg agggtcaact cgcgcttgtt cgcaaacgtt ccgttggtcg
     1801 cgtagacatc gatcatgggc atggagggct ccttctgtgc ttcactatga caatcgtcat
     1861 atcgacgccg cgatagtctc atatgatgac cgtcatagtc aaggagttac acgagaatga
     1921 gtagcgatag tcggcagaag atgatcgcca gcgccgcctc gttgattgca tcgcggggag
     1981 taaacgcgac gtcgttctcc gatgtcctcg ccgacagcgg cgctccccgc ggatcgatct
     2041 accatcactt cccggaaggg aagcggcaac tcgcggaaga tgccatccgt tggacatcga
     2101 gtcgcgttct tgccttcatg cgtgccggaa cgccaac
//
LOCUS       DASTMD010000034         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2144994, whole genome shotgun sequence.
ACCESSION   DASTMD010000034 DASTMD010000000
VERSION     DASTMD010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144994"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFQ54_00515"
     CDS             complement(<1..217)
                     /locus_tag="VFQ54_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163489.1"
                     /translation="MTGHLTELDVRRIVRDETAELREFVGIDDPRAGELRETLAALPG
                     ELSAIVSEARLRELRSRVAADRERDALT"
     gene            247..447
                     /locus_tag="VFQ54_00520"
     CDS             247..447
                     /locus_tag="VFQ54_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163490.1"
                     /translation="MSVGIYDLIVSIETTVQDAQHAVAKTPGIDATDMRFTQIRAELD
                     GLWNAMKVIGREIDRRSSNGAE"
     gene            508..792
                     /locus_tag="VFQ54_00525"
     CDS             508..792
                     /locus_tag="VFQ54_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163491.1"
                     /translation="MSRRRGNQSDLYIDILSGKSNIPREPDESPQPGDVTIHGNIPTP
                     ITDALVKIHNRAEDSIEDPTSPDAMLTRYHAAWEAILTLGAALDERNAAT"
     gene            789..1013
                     /locus_tag="VFQ54_00530"
     CDS             789..1013
                     /locus_tag="VFQ54_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163492.1"
                     /translation="MIAHSCRTLRKFHTEFEARCAGKPLDLFQRGTHLAAEAAADLRL
                     RHAGDLGDTILTDPLPEQLDAEVFNYPLSA"
     gene            complement(1465..1537)
                     /locus_tag="VFQ54_00535"
     tRNA            complement(1465..1537)
                     /locus_tag="VFQ54_00535"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1502..1504),aa:Asn,seq:gtt)
BASE COUNT          314 a          526 c          465 g          241 t
ORIGIN      
        1 gggtcagagc gtccctctcg cgatcggcgg cgacacggct ccgcagctca cggaggcgag
       61 cctccgaaac gatcgcggat agctcgccag ggagcgccgc gagcgtttcc cgcagctctc
      121 ccgcgcgcgg atcgtcaatc ccgacgaact cgcgcaactc ggctgtctca tcccgcacga
      181 tccgacgcac gtccaactcc gtcagatgcc cggtcacgaa acacgctccc cgataggctc
      241 accggcatga gcgtgggaat ttacgacctg atcgtcagca ttgagacgac cgtgcaagat
      301 gcgcagcacg ccgtcgccaa gacgcccggc atcgatgcga ccgacatgcg tttcacccag
      361 atccgagctg aactcgacgg gctctggaac gcgatgaaag tcatcggccg cgagatcgac
      421 cggagaagta gtaacggagc ggagtagcgg gcggcggatc gcggcccggg acacatcgcc
      481 gaagaccgtc tcgataagct cgcgtgcatg agcagacgac gcgggaatca gagcgacctg
      541 tacatcgaca tcctgagcgg gaagagcaac atcccccgcg agccggacga atcgccccag
      601 cccggtgacg tgacgattca cggcaacatt ccgacgccga tcacggacgc gctggtcaag
      661 atccacaacc gcgccgagga ctcgatcgag gatccgacgt ctcccgatgc gatgctgacc
      721 cgctatcacg cagcgtggga agcgatcctg acgttggggg ccgcgctcga tgaacggaac
      781 gccgccacat gatcgcgcac tcatgccgca cgctccgcaa gttccacacg gagttcgagg
      841 cccgctgcgc tggcaagccg ctcgatctct tccagcgtgg gactcacctc gccgctgagg
      901 cggcggctga cctgagattg agacatgccg gcgacctcgg cgacacgatc ctgactgatc
      961 cgcttccgga acagctcgac gcggaggtct tcaactatcc gctgagcgct tagcttctgg
     1021 ggcatggcgg cgacactatc cgctcagagc ctaagtagca agtcctaaat catgctcggt
     1081 gagtcaaatg cttgctgggg gagcagcttt cacccgctca gcgcttgcgt caaccgatgg
     1141 acgcgtgtag cgtctacgcc atgaccagca acgtcacgca cctcgtgagc cccgacaaca
     1201 cgcgctatga ggtcgccgca gccgtccgcg cccacctcgg agtgcatcgc atcagcgaca
     1261 gcgcgctcgg ccgcgcaatc ggtatgtcac agtcgatgat cagtcggcgt accaacggcg
     1321 agatcgcgtt cgataccgat gacctcggcc ggatcgctag ccacctcggc gtctcgatcg
     1381 tcgaactgat ccagatgccg accgcaacag cagaagcccc gcggcccggc ggcggggccg
     1441 gggcgcgagg cttctatctc tcggctcccc cacttggact cgaaccaaga acctgccggt
     1501 taacagccgg ctgctctgcc aattgagcta tggaggatca gccgtc
//
LOCUS       DASTMD010000035         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_538791, whole genome shotgun sequence.
ACCESSION   DASTMD010000035 DASTMD010000000
VERSION     DASTMD010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538791"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFQ54_00540"
     CDS             complement(<1..459)
                     /locus_tag="VFQ54_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HEU0163493.1"
                     /translation="MAAINIGDRRVNAKIVYYGPGLSGKTTNLINIHQRMPAGTRGAL
                     RSIETDAERTLFFDFVPVEPIRVGGWDVRFHLYSVPGQENYQRTRRAILGGADGIVFV
                     ADASEERLAANEASVDELGEHLESYGHTFDDLPVVFQFNKTDLPDALSTDD"
     gene            complement(461..1549)
                     /gene="purM"
                     /locus_tag="VFQ54_00545"
     CDS             complement(461..1549)
                     /gene="purM"
                     /locus_tag="VFQ54_00545"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU0163494.1"
                     /translation="MTQREFVKDSSGVTAGLTYKDAGVDIEAKTKAIDRLKLHAQATL
                     GNHAGPIGHFGGTYRMTTAGDQTLVASADGVGTKLRLAFLRGGEAHARVGRDIVNHCA
                     NDILALGATPLFFLDYFATGRLDPDVVDAVVGGVATACRESSIALLGGETAEMPDMYA
                     DGEYDLAGFIVGSVTDEKIVDGSKIEPGDAVIGLPSYGLQTNGYSLARKIVGLNGNAE
                     HDRELLAQPLPGSTTTIGDALMVGHESFVPVVQPLLAKGLVQGMAHITGGGLTDNLPR
                     TLPDDCIARLDPMQWDVPPIFRWLVEQGNLGVAERYHVFNMGIGFVLVAKPAEAREIL
                     ALVPQSRRIGQIVERMTSDESAVQGLSC"
     gene            complement(1635..>2236)
                     /locus_tag="VFQ54_00550"
     CDS             complement(1635..>2236)
                     /locus_tag="VFQ54_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163495.1"
                     /translation="NLPTQLIAAFRATLEEINEATVLVHVVDITHPTAGEQVATVKET
                     LEDLGVDGQPVVYALNKIEALSEEQLADLPNLMARIGLPADSVAISARDGLHIDTLFA
                     RIEGILETEDHFVPVTATIPYNQSELVERFHVLGRVEEREFDDVGTTLTGWLPERDLG
                     RFGSLIQRRTVSVPPAQVEDEIPVWETLPAEVQAAHPAA"
BASE COUNT          382 a          745 c          706 g          403 t
ORIGIN      
        1 gtcgtccgtg gaaagagcgt ccggaagatc ggtcttgttg aactggaaga ccaccggcag
       61 gtcatcgaac gtatgaccgt acgattccag gtgctccccg agttcgtcga cactcgcctc
      121 gtttgcggca agccgctcct cgctcgcatc cgccacgaag acgatcccgt ccgcgccacc
      181 gaggatcgca cggcgcgtcc gctggtagtt ctcctgtccc ggcacggagt agagatggaa
      241 ccgcacgtcc cacccaccaa cccgaatcgg ttcgaccgga acgaaatcga agaaaagcgt
      301 gcgctcggcg tcggtttcga tcgagcgcaa cgcaccacgc gttcccgccg gcattcgctg
      361 atggatgttg atgaggttgg tcgtcttgcc gctcaagccc ggaccgtaat aaacgatctt
      421 cgcgttgacg cggcggtcgc cgatattgat cgccgccatc tcagcaggaa agtccttgca
      481 cggcggactc gtcgctcgtc atccgctcga cgatctggcc gatgcggcgc gactgcggga
      541 ccagcgccag gatctcgcga gcttcggccg gcttcgccac caggacgaag ccgatgccca
      601 tgttgaagac gtgatagcgc tcggcgaccc cgaggttccc ttgctcgacg agccagcgaa
      661 agatcggcgg cacatcccac tgcatgggat cgagccgcgc gatgcaatcg tcgggaagcg
      721 tccgcggcag gttgtcggtg agaccaccac cggtgatgtg cgccattccc tggaccagcc
      781 ccttcgccag gagcggctgc acgaccggca cgaagctctc gtggcccacc atcagggcat
      841 cgccgatcgt cgtcgtcgat cccggaagcg gctgcgccag cagctcgcgg tcgtgctccg
      901 cattaccgtt cagaccgacg atcttgcgcg cgagactgta gccgttcgtc tggagaccgt
      961 agctcggcaa cccgatcaca gcgtcaccag gttcgatctt cgagccgtcg acgatcttct
     1021 cgtccgtgac cgaaccgacg atgaagccgg cgagatcgta ctcgccatcg gcgtacatgt
     1081 cgggcatctc ggccgtctcg ccgccgagca gggcgatcga gctctcccgg cacgcggtcg
     1141 cgacgccgcc cacgacggcg tcgacaacgt cgggatcgag tcgcccggtc gcgaagtaat
     1201 cgaggaagaa gagtggggtg gcgcccagcg ccaggatgtc gttcgcgcag tggttgacga
     1261 tgtcgcgacc gacgcgggcg tgcgcctcgc cgccacggag gaaggcgagg cgcagcttcg
     1321 tccccacgcc gtcggcgctc gcgacgaggg tctgatcacc ggcggtggtc atccggtagg
     1381 tgccgccaaa gtgaccgatc ggtccggcgt gattgcccaa tgtcgcttgc gcgtgcagtt
     1441 tcagccgatc gatcgccttc gtcttggctt cgatatcgac cccggcgtcc ttgtaggtca
     1501 ggcccgcggt caccccggac gaatccttca cgaactctcg ctgtgtcaca tggtttcctt
     1561 ctgggcaaca tcgatcactg aagatcggca gtgggacgag cgatccgcct gtcccactgc
     1621 ccgattgggc gcgatcacgc ggcaggatgg gctgcctgca cctcggcggg aagcgtctcc
     1681 cagaccggga tctcatcctc gacctgtgcc ggcgggacgc ttaccgtccg ccgctggatc
     1741 agggagccga aacgacccag atcccgttcc ggaagccatc cggtgagggt ggttcccacg
     1801 tcgtcgaatt cccgctcctc gacccgtccg agcacgtgga agcgctcgac cagttcggat
     1861 tgattgtacg ggatcgtcgc cgtgaccgga acgaaatgat cctcggtctc gaggatccct
     1921 tcgatccgtg cgaagagcgt gtcgatgtgc agtccgtccc gcgccgagat cgcaacggaa
     1981 tccgccggca ggccgatccg cgccatcagg ttcggcaggt cggccagctg ctcttcgctg
     2041 agcgcctcga tcttgttgag ggcgtagacg accggctgac cgtcaacgcc gaggtcctcg
     2101 agcgtctcct tgaccgtcgc gacctgctct ccggcggtcg gatgcgtgat gtcgacgaca
     2161 tgcacgagca ccgtcgcctc gttgatctcc tcgagcgtcg cccggaaggc cgcgatcaac
     2221 tgcgtcggga ggttgt
//
LOCUS       DASTMD010000036         1698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_628048, whole genome shotgun sequence.
ACCESSION   DASTMD010000036 DASTMD010000000
VERSION     DASTMD010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628048"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VFQ54_00555"
     CDS             <1..1067
                     /locus_tag="VFQ54_00555"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU0163496.1"
                     /translation="AGALIDEVINSSDVVSSVLDLARRNVIYMEDKDGKYTFELKDHK
                     ETLDPSEQQLVEAIFGLNGAAGAKVTMAQVQNTFVSWADRIHAGYYKGLVARKYFDVA
                     PDVARDRWKNAAFGIPVITIAIVVLILVLSGGTTGWMWFPIVVGAILTLVARQISKAM
                     PKKTLAGAEEAAKWRAFRKYLDDIEKYENLDESRKIFDKYLPYAVAFGLQSSWVAKFA
                     RVDMPMPQWYGGDWAGSMGQGRRVNRGGTWVLVGDPLGGGQRGSGGGNSGGGNSGGGG
                     GVDMPDLQEMSDSGGRSIQSASDSFAGMLDTAAKIFGGGGGGGKGGGSFGSWGGSRGG
                     GFSGGGGSGGSSGGGKRGFG"
     gene            1186..>1698
                     /locus_tag="VFQ54_00560"
     CDS             1186..>1698
                     /locus_tag="VFQ54_00560"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163497.1"
                     /translation="MSTTQALLHLTFIAIMIFLLVGGSVAILVLWYVRGRDPMVGPVA
                     AYLTEPPSDLSPGAAGTLIDENADHHDVLATLLGLGRFGTVEMRQISAKPGRTPVAGD
                     YQITVLEPDRIRSPLDRVLINALFANEPKAGDRVRLGEVRDRFARAEPMVRDALYQEL
                     VDRGYFLKSPA"
BASE COUNT          281 a          511 c          608 g          298 t
ORIGIN      
        1 cggcaggcgc cctgatcgac gaggtcatca attccagcga tgtcgtttcc tcggtgctcg
       61 atcttgcccg gcgcaacgtc atctacatgg aggacaagga cggcaagtac accttcgaac
      121 tgaaggacca caaggagacg ctcgatccct ccgagcaaca gctcgtggag gcgatcttcg
      181 gactcaatgg agcggcgggc gcgaaggtga cgatggcgca ggtgcagaac accttcgtct
      241 cctgggccga tcggattcat gcgggctact acaaggggct cgtggcccgg aagtacttcg
      301 acgtcgcgcc ggacgtcgcg cgcgatcgct ggaagaacgc cgcgttcggg attccggtga
      361 tcacgatcgc gatcgtcgtc ctgatcctcg tcctcagcgg cgggacgacg ggctggatgt
      421 ggttcccgat cgtcgtcggc gcgatcctga cgctcgttgc ccgccagatt tcgaaggcga
      481 tgccaaagaa gacgctcgcc ggagcggagg aagccgcgaa gtggcgtgcg ttcaggaaat
      541 acctcgatga catcgagaag tacgagaatc tcgatgagtc ccgcaagatc ttcgacaagt
      601 acctgccgta cgcggtcgcg ttcggcctgc agtcgagctg ggtcgccaag ttcgcccgcg
      661 tcgacatgcc gatgccgcag tggtatggcg gtgactgggc cggctcgatg ggccaggggc
      721 ggcgcgtgaa tcgcggcgga acctgggtcc tggtcggtga cccattgggt ggcggacagc
      781 gcggaagcgg cggtgggaac tccggcggcg gcaatagtgg cggcgggggc ggcgtcgata
      841 tgccggatct ccaggagatg agcgattccg gtgggcgttc catccagagc gccagcgaca
      901 gtttcgccgg gatgctggac accgccgcga agatattcgg aggcggcggc ggtggcggca
      961 agggcggcgg gagcttcgga agctggggtg gcagccgtgg cggtggattc agcggtggtg
     1021 gcggttccgg cgggagctcg ggcggtggca agcgcggctt cggatgatcg agtcgcgaat
     1081 cgcccgattc catcgccggg ccggtagagc accctcctgc cggttcggac cgctgattga
     1141 cggaccggcc ggagaacggg tggccgccag gctgggacga ttccgatgag taccacccag
     1201 gcgctcctgc atctcacctt catcgcgatc atgatcttcc tcctcgtggg aggatcggtc
     1261 gcgattcttg tcctgtggta cgtgcggggc cgggatccga tggtcgggcc ggtcgcggcc
     1321 tatctcaccg agccgccctc cgatctctcg cctggcgccg ccggcaccct gatcgatgag
     1381 aacgccgatc accacgatgt ccttgcgaca ttgctcggcc tcggtcgctt cggcacggtc
     1441 gagatgcggc agatctcggc gaagccggga cggacgccgg tggctggcga ctaccagatc
     1501 acggtgctcg agccggaccg gattcgctcg ccactcgatc gcgtgctgat caacgccctc
     1561 ttcgccaacg aaccgaaagc gggcgaccgg gtgcggctcg gcgaggtccg ggatcgcttt
     1621 gcgcgggcgg agccgatggt gcgcgatgcg ctgtatcagg agcttgtgga tcgcggctac
     1681 ttcctgaagt ctcccgcc
//
LOCUS       DASTMD010000037         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_895833, whole genome shotgun sequence.
ACCESSION   DASTMD010000037 DASTMD010000000
VERSION     DASTMD010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895833"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /gene="nuoD"
                     /locus_tag="VFQ54_00565"
     CDS             <1..1135
                     /gene="nuoD"
                     /locus_tag="VFQ54_00565"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEU0163498.1"
                     /translation="PSTHGVLRVLLKLDGEIVVDCDPVIGYLHRGTEKLGEAHRYAQL
                     TPWTDRTDYVAAPLNNLAYILTVEKLCDVEAPERAQYWRVIMAELSRLASHLVWLGTH
                     ALDIGALSMSLYCFREREIILDIFESFCGARLTTNMMEIGGFSRPIPDGLVHQIRNFL
                     RIFPGRQKEYATLLDVNPIWMSRTKGVGVISPEAAISYSLTGACLRGSGINYDVRKAT
                     PYLVYDRFDFEIPIGQHGDTYDRYLVRMEEMRQSIKIVTQALDHVEHMDSPLMAYESA
                     YVIPPHKGTMTTAEDMQRHFIWTIKGFNPPVGEAWVSIEHSKGEMGYYLVSDGGPMPY
                     RFRIRTPDFVNLSVLPHMAQGAMLADMVALIGTIDIVLGSVDR"
     gene            1301..2341
                     /gene="nuoH"
                     /locus_tag="VFQ54_00570"
     CDS             1301..2341
                     /gene="nuoH"
                     /locus_tag="VFQ54_00570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEU0163499.1"
                     /translation="MGEPIWVACIVAFIILNVLLISMAYMTWFERKVLAAMQDRLGPT
                     RSGPHGLFQPLADAVKLLGKEDLVPAAADRYVFLLAPVAVFTAALTAASVIPFGGTIN
                     LWGHHVNLYATDLNVGTLFILAVGSAGVYGIILGAWASANRFSLLGGLRSAAQVVSYE
                     LILGFSLVGIFILSGTLSLRGIMEAQHKSLSIGPFDISNWYILSQPLAFVLFLIAAVA
                     ETNRAPFDLPEAETELVAGFHTEYSGFRFSLYFLGEYINMIVTSLFASTLFLGGTDGP
                     GSQKWWGLGILWLFLKTIAFLFFYVWLRGTLPRFRFDQLMGIAWKVLLPLALLNIIVT
                     AFIRIIANGQVF"
     gene            2415..>2840
                     /locus_tag="VFQ54_00575"
     CDS             2415..>2840
                     /locus_tag="VFQ54_00575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU0163500.1"
                     /translation="MPWDVIFFYVIGTVCILSAIGMVVSPNPVHSGIFLVLCFLNVAA
                     IFVMLGAEFLAVIQIIVYTGAILILMLFVLMLVDPEQLPAFSSARPLQRNLSFLLGLI
                     LLLEIGAAIITRTSIGPVGKDTPAAVSAVGGNVQAIGISI"
BASE COUNT          442 a          979 c          834 g          585 t
ORIGIN      
        1 cccgagcacc catggcgtgc tgcgcgtgct gctcaagctc gacggcgaga ttgtcgtcga
       61 ttgcgaccct gtgatcggct acctccaccg cggtacagag aagctcggtg aggcccaccg
      121 gtacgcgcag ctgacgccgt ggacggatcg caccgactac gtcgccgcgc cgctgaacaa
      181 cctcgcctac atcctcaccg tcgagaagct ctgcgacgtc gaggcgccgg agcgcgcgca
      241 gtactggcgt gtgatcatgg cggaactgag ccgcctcgcc tcccatctcg tctggctcgg
      301 cacccacgcc ctggatatcg gggcgctctc gatgtcgctc tactgcttcc gggagcgcga
      361 gatcatcctc gacatcttcg agtccttctg cggcgcccgc ctcaccacga acatgatgga
      421 gatcggcggc ttctcgcgac cgattccgga cggcctcgtc catcagatcc gcaacttcct
      481 gcggatcttc cccggccgcc agaaggaata cgcgacgctg ctcgacgtca acccgatctg
      541 gatgtcccgc acgaagggcg tcggcgtcat ctcaccggag gccgcgatct cctactcgct
      601 caccggcgct tgtctccggg gatccggcat caactatgac gtccgcaagg cgacgcccta
      661 cctcgtctac gatcggttcg acttcgagat cccgatcggg cagcatggcg acacctacga
      721 ccgctatctc gtccgtatgg aggagatgcg ccagtcgatc aagatcgtca cccaggcgct
      781 cgaccatgtg gagcacatgg attcgccgct gatggcgtac gaatccgcct acgtgatccc
      841 gccgcacaag ggcaccatga cgaccgccga ggatatgcaa cggcatttca tctggacgat
      901 caaggggttc aatccgccgg ttggcgaggc gtgggtctcg atcgagcatt cgaaggggga
      961 gatgggctac tatctcgtct ccgacggcgg tcccatgcca taccgcttcc ggatccggac
     1021 tcccgatttc gtgaatcttt ccgtcctgcc acacatggcc cagggagcca tgctggcgga
     1081 catggtcgcc ctcatcggca cgatcgatat cgtgctcggg tcggtcgacc gctagccgcg
     1141 cagattgggc cggctccgat gccgggcagg gaacggtggg acgatcggat ccggattggt
     1201 ggagcgtctc cgtccagtcg cgggaaactt tggggagagg cgacggcgcg gttccgagct
     1261 ggagagcgtg cctgcattcg tcgagggctg aaccaacatc atgggcgaac caatctgggt
     1321 tgcatgcatt gtggccttca tcatcctgaa cgtcctgctg atcagcatgg cgtacatgac
     1381 ctggttcgag cgcaaggtgc tcgcggccat gcaggatcgt cttggcccga cgcgctccgg
     1441 tccgcacggc ctcttccagc cgctcgcgga cgccgtgaag cttctcggca aggaggacct
     1501 cgtccccgcg gcggctgacc ggtacgtctt cctgctcgcc ccggtcgccg tcttcaccgc
     1561 cgccctcacc gccgcgtccg tcattccctt cggcggcacg atcaatctct ggggccacca
     1621 cgtcaatctc tacgcgaccg atctcaacgt ggggacgctc ttcatcctcg cggttggatc
     1681 ggcgggcgtc tacggcatca tcctcggcgc ctgggcgtcg gcgaaccgct tctcgctgct
     1741 gggcggactc cgttccgccg cgcaggtcgt ctcctacgag ctcatcctcg gcttctcgct
     1801 ggtggggatc ttcatcctct ccggcacgct cagtctccgt ggcatcatgg aggcgcagca
     1861 caagtcgctc tcgatcgggc cgttcgacat cagcaactgg tacatcctct cccagccact
     1921 tgccttcgtc ctcttcctga tcgccgccgt tgcggagacg aaccgcgcgc cgttcgacct
     1981 cccggaggcg gagaccgaac tcgtcgccgg tttccacacc gagtattccg ggttccgctt
     2041 ctcgctctac ttcctcggcg aatacatcaa catgatcgtg acctcgctct tcgcgagcac
     2101 gctcttcctc ggcggcaccg atggaccggg ctcccagaaa tggtggggac tcgggatcct
     2161 ctggctcttc ctgaagacga tcgccttcct cttcttctac gtctggctcc gcgggacgct
     2221 gccgcgcttc cgcttcgacc agttgatggg gatcgcctgg aaggtgcttc tgccgctggc
     2281 gctgctgaac atcatcgtca ccgcctttat ccggatcatc gcgaacgggc aggtcttctg
     2341 atcatgagcg ccttacgcct ccagcacctt tcatccagat gcgacaggtc ggacctcgtg
     2401 ggaggaatcg cctgatgccc tgggatgtca tcttcttcta cgtgatcggg acggtctgca
     2461 tcctctcggc gatcgggatg gtcgtttcgc cgaaccccgt ccactccggc atcttcctgg
     2521 tgctctgctt cctgaacgtg gcggcgatct tcgtgatgct cggcgcggaa ttcctcgccg
     2581 tgatccagat catcgtctac accggcgcga tcctgatcct gatgctcttc gtgctgatgc
     2641 tcgtcgatcc cgagcagttg cccgccttct cctcggcgcg accgctccag cgcaacctga
     2701 gcttcctgct cggtctgatc ctcctcctcg agatcggcgc cgcgatcatc acccgcacct
     2761 cgatcgggcc ggtcggcaag gacacgccgg cggcggtctc ggcggttggt ggcaacgtcc
     2821 aggcgatcgg gatctcgatc
//
LOCUS       DASTMD010000038         1975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_271521, whole genome shotgun sequence.
ACCESSION   DASTMD010000038 DASTMD010000000
VERSION     DASTMD010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271521"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFQ54_00580"
     CDS             complement(<1..621)
                     /locus_tag="VFQ54_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163501.1"
                     /translation="MSHRRMRLTLSLIVVLLASTLSGGMPASAAAQSVSATHQRDISL
                     DGTPVAISPDGKRIAGLGPDHTFCVWRISTRKATCSDEISSITPDTIRWAPDSSAVAF
                     SQNAAINFVDSDIYVFDIASGKVKDVTDDSYDGNLSLDTDTETRATINVDINPGWSPD
                     GKDLIFARTVWGTATGMGSQLMKVSRDGGDPVTLFTVAPPEPLVLWG"
     gene            complement(693..>1975)
                     /locus_tag="VFQ54_00585"
     CDS             complement(693..>1975)
                     /locus_tag="VFQ54_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163502.1"
                     /translation="GKKVVIIINKIDLLRDDASKQQVVTFVRENIEKLLGFRPKVFPV
                     SALLAQQAKQLADRNPAERDRLWTASQFEPLEHYVIDTLDEESRIRLKLLNPLGIADH
                     LAGRYAKATQDRLSVLADDLTTIENIDRQLTLYRDDMRRQFAYHLTRIENIIHHMNNR
                     GDEFFDETIRIGRIFDLLNPDKIRVLFERSVVGDTEQAIDTSIDELIDWMVEQDLRVW
                     EAVNEYIDRRRLNKYADELIGEVGGQFRYDRRSMLESVAKRAQEEVDRYDMDTHARDL
                     SLSVRNAVATVAVAEAGAVGLGALIVAAASTAVVDVTGILAAGVVAGLGLVILPARRR
                     RAKQELHKRSAELEKRLTTVMGEQFEHELNRSVTRIVDAIAPYTRFVRSSQEKLLGIE
                     GDLSAIQTELRSLRREIGGEDQVTAVPETTNRTS"
BASE COUNT          327 a          601 c          646 g          401 t
ORIGIN      
        1 cccccacagg acaagtggtt caggcggcgc cacggtgaac agggtgacgg gatccccacc
       61 atcgcgcgat accttcatta gttggctgcc catgccggtc gccgtccccc acaccgtgcg
      121 cgcgaagatc aggtccttgc cgtcgggcga ccagcctgga ttgatatcga cgttgatggt
      181 ggccctggtc tccgtatcgg tatcgagcga gagattcccg tcgtatgaat cgtcggtaac
      241 gtccttcacc ttccccgatg cgatgtcgaa gacgtagatg tcgctgtcga cgaagttgat
      301 cgcggcattc tgggagaagg cgacggcgct gctatccggc gcccatcgaa tggtgtccgg
      361 cgtgatcgac gatatctcgt cgctgcacgt tgccttcctg gtggatatgc gccagacaca
      421 gaaggtgtga tcgggaccga ggccggcgat ccgtttgcca tcgggcgaaa tcgcgaccgg
      481 tgttccatcc agtgagatat cgcgttgatg tgtggcgctc acactctggg cggcggcgga
      541 ggccggcatc ccgccggaga gggtgctcgc cagcaggacc acgatgagtg agagcgtcag
      601 gcgcatgcgt cgatgtgaca tcaggaagtt cctcccgttc gcaaattgac tcaccgtgga
      661 tacgttgcga cccggttgga ttgttccgat ggtcaggatg tccggttcgt ggtttccgga
      721 acggccgtca cctggtcctc gccgccgatc tcccggcgca acgagcgaag ttcggtctgg
      781 atcgcggaga gatccccttc gatcccgagc agcttctcct gcgacgagcg gacgaagcgc
      841 gtgtacggcg cgatcgcgtc gacgatccgg gtcaccgagc gattgagttc atgttcgaac
      901 tgctcaccca tcaccgtcgt caaccgcttc tcgagttccg cggaccgctt gtgcagctcc
      961 tgcttcgccc ggcgtcgccg cgccggcagg atgacgaggc cgagtccggc gacgaccccg
     1021 gcggcgagaa tgcccgtgac atcgacgact gccgtgcttg ccgccgccac gatcagcgcg
     1081 ccgagaccga ccgcgcccgc ctccgccacg gcgaccgtcg cgaccgcgtt gcggaccgag
     1141 agcgagagat cgcgcgcatg ggtgtccatg tcgtagcggt cgacttcttc ctgggcccgc
     1201 ttcgcgaccg actcgagcat cgagcggcgg tcgtagcgga actggccgcc gacctcgccg
     1261 atcagttcgt ccgcgtactt gttgaggcga cggcgatcga tgtactcgtt gacggcttcc
     1321 cagacgcgga gatcctgttc gaccatccag tcgatcagct cgtcgatgct ggtgtcgatc
     1381 gcctgctcgg tatcgccgac gacggagcgc tcgaagagga cgcggatctt gtccggattg
     1441 aggagatcga agatgcggcc gatccggatc gtctcatcga agaactcgtc accccggttg
     1501 ttcatgtggt ggatgatgtt ctcgatgcgt gtcaggtgat aggcgaactg ccggcgcatg
     1561 tcgtcgcgat agagcgtcag ctggcggtcg atgttctcga tcgtcgtgag gtcgtcggcg
     1621 aggacgctga ggcgatcctg ggtcgccttg gcgtagcgtc cggcgagatg atcggcgatg
     1681 ccgagcggat tcagcagctt gagccggatc ctgctttcct catcgagcgt gtcgatcaca
     1741 tagtgctcga gcggctcgaa ctggcttgcc gtccagagcc gatcgcgttc tgctggattg
     1801 cggtcggcga gttgcttcgc ctgctgggca agcagcgccg agaccgggaa gaccttcggt
     1861 cggaagccaa gcagtttctc gatgttctcg cgaacgaagg tcacgacctg ctgtttcgag
     1921 gcgtcgtccc gcaggaggtc gatcttgttg atgatgatga cgaccttctt gcccc
//
LOCUS       DASTMD010000039         3989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_271572, whole genome shotgun sequence.
ACCESSION   DASTMD010000039 DASTMD010000000
VERSION     DASTMD010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271572"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="VFQ54_00590"
     CDS             <1..1269
                     /locus_tag="VFQ54_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU0163503.1"
                     /translation="RRNEMVAHIRARHTQILEGISIEDAHASARAAIDALHIALDESL
                     DEIAADGELTDRLSDRVVATGEKCMSVLMSATIRELGGASAPVFADTVIATDERHGSA
                     RPDRALTRERADSVIRPLLDAGNTVVATGFIGAGPDGATTTLGRGGSDYSATLLGAAL
                     DADEVQIWTDVPGVLSADPRKVARARVVPEISYDEAQELAHFGAKVLHPRTIRPAVAL
                     NIPVHILSTFAPDEPGTIVTQESSGNHVKAVTALKGLILLTLDVPELEDLAPAASATF
                     RILDEQHIEIVTVSQASSRRRMTFLVDAVLGGCARLQTRIEEQLGDHEVTVTCSEDVA
                     AVAAVGAGAAGTPSALSAMLTALHRANVPVLATNQQTSNVAMLVVIPEEDADRAVAAL
                     HDRLIRPQLASNRDRRRRRSDLMAESFRVG"
     gene            1332..2393
                     /gene="asd"
                     /locus_tag="VFQ54_00595"
     CDS             1332..2393
                     /gene="asd"
                     /locus_tag="VFQ54_00595"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU0163504.1"
                     /translation="MSDGRIPVAVLGATGNVGQRFVQLLENHPWFRVAELVASERSAG
                     KKFSEATDWRLGPDMPAAAADLPVLDYHADLQSPVVFSGLPGEVAEEIEQRLAARGHA
                     VLSNTSTHRMEPDVPLLIPEVNSDHMAAIATQRANRGWKGYIVTNANCSAIHLTLALK
                     PLHDAYGLDAVLVTTMQAVSGAGYPGVPSLDAIDNVVPYISNEEEKLETESQKMLGGY
                     TAEGGFAPADFVLSAHCNRVTVRDGHLETVSVRFKNPATPAEVIETFESFRARPQELD
                     LPSAPPQPIIVRREINRPQPIFDRNASNGMASVVGRVRECPLLGIKFVVLGHNTIRGA
                     AGASILNAELFKVEGLLPA"
     gene            complement(2415..2975)
                     /locus_tag="VFQ54_00600"
     CDS             complement(2415..2975)
                     /locus_tag="VFQ54_00600"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0163505.1"
                     /translation="MVRRTRLVVIGGSPGSGKTTLARELGERLRFPVIARDAIKEVLL
                     DSLPPRDRTESMRLGGASWPMMYAVLDAMIDRVPGVILESNFRRGRSEAELLPRVARC
                     ESLLIQCEASWESIEPRIQARENDPHRHPGHFDQVALPEVKASFDRGEFAPMGLGIPV
                     VAVSTDDGYEPALDDLIGIIERRANS"
     gene            3132..>3989
                     /locus_tag="VFQ54_00605"
     CDS             3132..>3989
                     /locus_tag="VFQ54_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163506.1"
                     /translation="MAFFDHTQVRDPSWRERSWMILAIAILLMPFTASQAIAHGDAGT
                     PIPGSSPDCAQGDAATWTRQMDAYAAAGSYPATQTNGATGPSSVLVDIPPIAFTGDGK
                     SATLPVGPCQTIYRFAYSNITSAAGDPGPFIYVEIDWNTEGIPRGPNGSFISPHFDFH
                     FYMLSRQQIDQQVDCVSTNGRTCDPFQTSYTQTQLFQDLPDAEFIPSTYKPDIDSAIP
                     EMGLHLLDMSIDYTVDNVNHYPTLIYGTFDSKVIFAESSVTLYTLQDAMAAPGNRISF
                     PFRQPDAFAT"
BASE COUNT          714 a         1439 c         1172 g          664 t
ORIGIN      
        1 agacgcaacg agatggtggc ccacatccgg gctcgccaca cccagatcct cgagggcatc
       61 tcgatcgaag atgcccatgc gtccgcccgc gccgcgatcg acgccctcca catcgcgctc
      121 gacgagagcc tcgacgagat cgccgcggac ggcgaactga cggatcgtct ctccgaccgc
      181 gtcgtcgcaa cgggcgagaa gtgcatgagc gtcctgatga gcgcgacgat ccgcgaactc
      241 ggcggcgcct cggccccggt cttcgccgac accgtgatcg ccaccgacga acgccacggc
      301 tccgcccgcc ccgatcgcgc cctgacccgg gaacgtgccg attccgtgat tcgcccgctc
      361 ctcgacgcgg gcaacacggt cgtcgccacc ggattcatcg gcgccggtcc tgacggcgcg
      421 acgacgacgc tcgggcgcgg cggctccgat tacagcgcga cactcctcgg ggcggcgctc
      481 gatgccgatg aggtccagat ctggaccgac gtgcccggcg tcctctccgc cgatccccgc
      541 aaggtcgccc gcgcccgcgt cgttccggag atcagctacg acgaagcgca ggaactcgcc
      601 cactttggcg cgaaggtcct ccacccgcgc acgatccgcc cggcggtcgc cctcaacatc
      661 ccggttcaca tcctctccac cttcgctccc gacgaaccgg gcacgatcgt cacccaggaa
      721 tcatccggca accacgtcaa ggcggtgacg gcactcaagg ggctgatcct cctcacgctc
      781 gacgtcccgg aactcgagga cctcgcgccc gcagcctccg ccacctttcg catcctcgac
      841 gaacagcaca tcgagatcgt caccgtcagc caggcctcgt cccggcgccg gatgacgttc
      901 ctcgtcgacg ccgtcctcgg cggctgcgcg cgactccaga cgcggatcga ggagcagctc
      961 ggcgatcatg aagtcacggt cacgtgctcc gaagacgtcg ccgccgtcgc ggcggtcggt
     1021 gccggcgcgg cgggaactcc gtccgcgctc tcggcgatgt tgaccgcgct ccaccgcgcg
     1081 aacgtccccg tcctggcgac gaaccagcag acgtcgaacg tcgcgatgct cgtcgtcatt
     1141 ccggaagaag acgccgatcg cgcggtcgcc gcgctgcacg atcggctcat ccggccccaa
     1201 ctcgcatcca accgcgaccg gcggcgtcgc cgctcggatc tcatggcgga gtcattccgc
     1261 gtcggctgat cgactgtcgt ttgtacgagg tcgacacggg gtcgacctga gctttcagga
     1321 ggatccacaa cgtgtcagac ggacgtattc cggtcgcagt actcggcgcc acaggcaatg
     1381 tcggccagcg cttcgttcaa ctcctcgaga atcacccctg gttccgcgtc gccgaactcg
     1441 tcgcctccga acgatcggcg ggcaagaagt tctccgaagc gaccgattgg cgtctcggcc
     1501 cggacatgcc ggcggcggcc gccgatctcc ccgtcctcga ctaccacgcc gacctgcaga
     1561 gtcctgtcgt cttttccggt ctccccggcg aagttgcgga ggagatcgaa cagcgtctcg
     1621 ccgcgcgcgg gcacgccgtg ctcagcaaca cgtcgaccca tcgcatggag cccgacgttc
     1681 ccctcctgat cccggaagtg aattccgatc acatggccgc gatcgcgacg cagcgggcga
     1741 atcgtggctg gaagggctac atcgtcacca acgcgaactg ctccgccatc catctgacgc
     1801 tcgcgttgaa gccgctccat gacgcctacg ggctcgatgc ggtcctcgtg acgacgatgc
     1861 aggctgtctc cggcgcggga tatcccggtg tgccgtcgct cgatgcgatc gacaacgtcg
     1921 tgccgtacat cagcaacgag gaagagaagc tcgagacgga atcgcagaag atgcttggtg
     1981 gctacacggc cgagggcggc ttcgccccgg ccgacttcgt gctgagtgca cactgcaatc
     2041 gcgtcaccgt ccgcgacggc catctcgaga ccgtctcggt gcggttcaag aacccggcga
     2101 cgcctgccga ggtcatcgag accttcgagt ccttccgcgc ccggccgcag gaactcgatc
     2161 tgccgagcgc gccaccgcaa ccgatcatcg ttcgccgtga gatcaaccgc ccgcagccga
     2221 tcttcgatcg caacgcgtcg aacgggatgg cttcggtcgt cggtcgcgtt cgcgagtgcc
     2281 cgctgctggg catcaagttc gtggtgctcg gccacaacac gatccggggc gcggccggcg
     2341 cctcgatcct caatgcggaa cttttcaagg tcgagggcct gcttcccgcc tagccgcaaa
     2401 ccatcaatcc aggtttagga attcgcccgg cgctcgatga tcccgatcag atcgtcgagc
     2461 gccggttcgt atccgtcgtc ggtcgaaacg gcaacgaccg ggatcccaag acccatcggc
     2521 gcgaattcgc cacgatcgaa cgacgccttc acctctggca acgcgacctg gtcgaaatga
     2581 ccgggatggc ggtgcgggtc gttctcgcgc gcctggatcc gcggctcgat cgattcccac
     2641 gacgcctcgc actggatgag cagactctcg caccgcgcga cgcgaggcag gagttccgcc
     2701 tcggaacgac ccctccggaa gttgctctcg agaatcacac ccggcacgcg gtcgatcatc
     2761 gcgtcgagaa cggcatacat catcggccag gatgcgccac cgagccgcat cgattctgtg
     2821 cggtctcgtg gcggcaggga gtcgaggagc acttccttga tcgcatcccg cgcgatcacc
     2881 gggaagcgaa gccgttcgcc gagttcgcgc gcgagcgtcg tcttcccact ccccggcgag
     2941 ccgccgatga ccacgagccg tgtacgtcga accatgaaag gaacctcact ccccagagat
     3001 gtcgcgttct ggcggggcga cgatcgatcc acattgatgc cccatagaca catttcttcc
     3061 caaaatctat gctgaacgct ggagcgggac cctcgaccag atacgcgatg ttcaagatac
     3121 gggaggacca gttggctttc ttcgatcaca cgcaggtgcg cgatccatcg tggcgcgagc
     3181 ggtcatggat gatcctcgcg atcgcgatcc tactgatgcc cttcaccgcg agccaggcga
     3241 tcgcacatgg cgacgccggc actcccatcc ccggatcgtc tcccgactgc gcccagggag
     3301 atgcggcgac atggactcgc caaatggacg cctacgcggc ggccgggtcc taccctgcca
     3361 cgcagaccaa cggcgcgacc ggcccatcga gcgttctcgt cgatatcccg ccgatcgcct
     3421 ttacgggtga cggcaaatcg gccacgctcc cggtcggccc ctgccagacc atctatcgct
     3481 tcgcctactc gaacatcacg tcggcagcgg gagatcccgg gcccttcatc tacgtggaga
     3541 tcgactggaa caccgaaggg attccccggg gcccgaacgg gtcgttcatc tccccccact
     3601 tcgacttcca tttctacatg ctgtcgcggc agcagatcga ccagcaggtc gactgcgtct
     3661 ccacgaacgg tcggacgtgc gacccgttcc agaccagcta tacccagacg cagcttttcc
     3721 aggatctccc ggacgcggaa ttcatcccgt cgacgtacaa acccgatatc gactccgcga
     3781 ttccggagat ggggctgcat ctgctcgaca tgagcatcga ctacacggtc gacaatgtga
     3841 accactatcc gaccctgatc tatggcacgt tcgacagcaa ggtgatcttc gccgaatcct
     3901 cggtcaccct ctatacgctc caggacgcga tggcggcgcc aggcaaccgg atctcgttcc
     3961 ccttccggca accggacgcc ttcgcgacc
//
LOCUS       DASTMD010000040         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1967299, whole genome shotgun sequence.
ACCESSION   DASTMD010000040 DASTMD010000000
VERSION     DASTMD010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967299"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="VFQ54_00610"
     CDS             complement(<1..1245)
                     /locus_tag="VFQ54_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU0163507.1"
                     /translation="MLPGLEDPISTTLGLVAVVALVLANGFFVATEFALVSVRRTRIQ
                     QLAAEGDRRATGVLGLLDHLDTYIAATQLGITISSLALGWVGEPAVASLLHGVLHGLP
                     FEIGSTETHAISFVVAFSLVTAMHIVIGELAPKSLALQHSEATVMAVYRPIQVFQTIF
                     RPVIAFLNWIGNRVVTLAGINPASGHALVQSAEELMLAVDASREAGLVNQTAHDIVGR
                     ALSFTDMQARHVMVPRTEVTALPIDATLDDVIQISTEASHSRLPVFEGDNDHIVGIIK
                     VKRLLPLILQRATAANGAAQMRGIGVAGVTSTPPQFDIREYMMEPMLVPETLAASEVL
                     TQMRENGVQIAVVIDEYGGTAGIVTLQDIVTHLVGRIQEEEELTVDGLDLNGVLHIDG
                     LTSLVELRERFGIDLDDEQYDVE"
     gene            1493..2119
                     /locus_tag="VFQ54_00615"
     CDS             1493..2119
                     /locus_tag="VFQ54_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0163508.1"
                     /translation="MAVLQSVEPINRPKDPLAYLAELLSSRVRAQVITFLAVRSDTAY
                     SLTEIARGLGLSISSVQHECYKLERLNVLIARPDRGSRRYQLNRGFATTEPLVSMVFS
                     AFTPVHLLGLSLEDAVDDGSLISAALAGDPQAAAEAPLHLVLIGTLSLDQLASIQDRA
                     PQLLGIPPESLEVAFFSRDQWDSHVAAGHEFVTRLLREPLLPIVGSLQ"
BASE COUNT          415 a          736 c          705 g          415 t
ORIGIN      
        1 ctctacatcg tactgctcgt catcgagatc gatcccgaat cgttcccgga gttcgacgag
       61 gctcgtcagg ccgtcgatgt gaagaacgcc attgagatcg agaccatcga ccgtcagttc
      121 ttcctcctcc tggatgcgac caacgaggtg ggtgacgatg tcctgcaggg tcacgatgcc
      181 ggcggtgccg ccgtattcat cgatcacgac ggcgatctgg acgccgttct cgcgcatctg
      241 cgtcagcacc tcggaagctg cgagggtctc cggcacgagc atcggctcca tcatgtattc
      301 gcggatgtcg aactgcggcg gcgtgctcgt caccccggcg acgccgattc ccctcatctg
      361 ggcggcgccg ttggccgccg tcgcccgctg caggatcagc ggcaggagac gcttgacctt
      421 gatgatgccg acgatgtggt cgttgtcgcc ctcgaagaca ggaagccggg aatgggacgc
      481 ctcggtcgag atctggatca catcgtccag cgtcgcgtcg atcggcagcg ccgtcacttc
      541 agtgcgcggc accatgacgt gacgtgcctg catgtcggtg aacgaaaggg cacgcccgac
      601 gatgtcgtga gcggtctgat tgacgagtcc cgcctcacgg ctggcgtcga cggcgagcat
      661 caactcctcc gccgactgca cgagcgcgtg tcccgacgcc gggttgatgc cggcgagcgt
      721 gacgaccctg ttgccgatcc agttcaggaa ggcaatgacg ggccggaaga tcgtctgaaa
      781 aacctggatc ggccggtaga ccgccatcac ggtcgcctcg gagtgttgca gcgcgaggct
      841 cttcggcgcg agttcgccga tcacgatgtg catcgcggtc acgagggaga acgcgacgac
      901 gaacgagata gcgtgggttt ccgtggagcc gatctcgaac ggcagtccgt gaaggacccc
      961 gtggagcagg ctggcgacgg cgggttcacc gacccaaccg agcgcaaggc tcgagatcgt
     1021 gatgccgagc tgcgtcgcgg cgatgtaggt gtcgaggtga tcgagcaatc cgagcacacc
     1081 ggtcgcgcgg cgatcgcctt cggcagcgag ttgttgaatg cgggtgcggc ggacagaaac
     1141 gagggcgaac tcggttgcga caaagaagcc gttggccaag acgagcgcaa cgacagcaac
     1201 gagtcctaac gtggtactaa ttgggtcctc cagaccgggt aacagatgcg cgaattcgcc
     1261 gcccgcgcca gcggtatgtc tgtggcgatt gccacgacag agttacttca tcttagagga
     1321 aagaaggcaa aatctccacg gttcttccat acagatgcac catcaggcac acgtcggttg
     1381 tcaccggatg tccggatatc atgcacggtg cacgatgcgt agggcaaaat ggagccggca
     1441 ctggcctgaa ccattgcgtg ccgttcctgt gggagagacg aggcgtacga ccgtggcagt
     1501 gttgcaatcc gttgagccga tcaaccgacc gaaggatccg ctcgcctatc tggccgaact
     1561 gctctcttcc cgcgtgcggg cgcaggtcat cacctttctc gccgtacgat ccgataccgc
     1621 ctactccctc accgaaatcg cccgcggcct ggggctctcg atcagcagcg tgcagcatga
     1681 gtgctacaag ctcgagcgcc tcaacgtcct catcgcgcgt cctgaccggg gttcccgtcg
     1741 ctatcagctc aatcgcggct ttgccaccac ggaacccctc gtctcgatgg tcttctcggc
     1801 cttcaccccc gttcatctgc tggggctctc gctcgaagac gcggtcgacg acggttcgct
     1861 gatctccgcg gcgctcgcgg gcgatcccca ggccgcggcc gaagcaccgc tccacctcgt
     1921 cctcatcggc acgctctcgc tcgatcagct ggcgagcatc caggaccgcg caccacagct
     1981 gctcggcatc cctcccgaat cactcgaggt cgccttcttc agccgcgatc aatgggattc
     2041 ccatgtcgcg gccggtcatg aattcgtcac gcggcttctc cgtgaaccac tgctgccgat
     2101 cgtgggatcg ctccaatgat cccgattctc ctcgaggaaa cgagtcgatc atgacccaca
     2161 cacgcagggc gaccgcggca ccgcaggcgc ccgtcctcat cgttggccag tccggcgggg
     2221 cgaccgcggc gatcaacgcg agcctgatcg gggtcgtcga tgccgccctc g
//
LOCUS       DASTMD010000041         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2860319, whole genome shotgun sequence.
ACCESSION   DASTMD010000041 DASTMD010000000
VERSION     DASTMD010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2860319"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFQ54_00620"
     CDS             complement(<1..616)
                     /locus_tag="VFQ54_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU0163509.1"
                     /translation="MTQHDWSSAEFETRAIHAGQDPDSATGAVITPIYQTSTYAQEEI
                     GVTKGYEYSRTDNPTRTALQSALASLEGGAWALAYASGLAATQNLGYLLRPGDHVLLS
                     NDSYGGTYRLFAQVLQNYGINFSIADLSNLDEVRAGLRPATKFVWVETPTNPYLRIVD
                     IAGIAAAIAGTTARLVVDNTFASPYLQNPLRFGADFVLHSTTKYI"
     gene            complement(671..2170)
                     /locus_tag="VFQ54_00625"
     CDS             complement(671..2170)
                     /locus_tag="VFQ54_00625"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEU0163510.1"
                     /translation="MSSSVAGPPGAVGTAAPRRRMFDNVIEAMGDTPIVRLNHITQGI
                     RTPIYAKLEMMNPGGSVKDRIGIRMIEEAERRGWLRPGGTIVEPTSGNTGVGLAMAAA
                     VKGYHCIFVMPDKVSAEKVQLLRAYGAEVVTTPTSVERDSPESYYSVADRLTREVPNA
                     FQPNQYFNPQNPRTHYESTGPEIWEQTDGKVTHFVAGVGTGGTISGTGRFLKEKNPAI
                     RIVGADPEGSIYTSQIIHQYKVEGVGEDFWPGSFDRNIVDQWIQVNDTEALLMARRVT
                     REEGILVGGSCGLAVHAALELGKRLDDPDALIVVLLPDSGRGYLSKIYNDDWMRENGF
                     LSRFSSPSRMGNLIDARSGGVPHVVAVGCDQTVRQAIELLREYEVSQLPVVRGASSSQ
                     TRGEDRSRDCLGPEPGETIEVHGIVGSLQERTLLDHIFRNPEVMNERVSTVMDGPFNL
                     VDANEEIERVIPILAANAPAVLVQRDGVLIGVITRADILDYVAHQRQTR"
BASE COUNT          423 a          770 c          765 g          454 t
ORIGIN      
        1 cgatgtattt cgtggtgctg tggagcacga agtcggctcc gaacctgagc ggattctgca
       61 gatacggtga cgcgaacgtg ttgtccacga cgagccgcgc ggtcgtcccc gcgatcgccg
      121 cggcaatccc ggctatatcg acgatgcgca ggtacggatt cgtcggcgtc tcgacccaga
      181 cgaacttcgt cgccggacgc agccccgcgc gaacttcatc gaggttgctc agatccgcga
      241 tcgagaagtt gatcccgtag ttctggagca cctgcgcgaa gagccggtag gtcccaccgt
      301 aggagtcgtt cgagagcagg acgtgatcgc cgggacgcag cagatacccg aggttctggg
      361 tcgcggcgag tccgctcgca tacgcgagcg cccaggcacc gccttcaagc gaggcgagcg
      421 ccgattgcaa cgccgttcgc gtcgggttgt ccgtgcggga gtactcgtac cccttggtga
      481 cgccgatctc ttcctgcgcg tacgtgctgg tctggtagat cggtgtgatc accgcgcctg
      541 tcgcgctatc cggatcctgc ccggcgtgaa tcgcgcgcgt ttcgaactcg gcgctggacc
      601 aatcgtgctg agtcatgatg tctcccggga tctatatggc gtttcggaca gacagggacg
      661 atccgcgcga ctaccgcgtc tggcgctgat gagcgacgta atcgaggatg tcggcccggg
      721 taatgacgcc gatcaggacg ccgtcccgct gcacgagcac cgccggtgcg ttcgccgcga
      781 gaatcgggat cacgcgctcg atctcctcgt tcgcatcgac cagattgaac gggccgtcca
      841 tgacggtact gacgcgctcg ttcatcacct ccgggttccg gaagatatga tcgagcagag
      901 ttcgttcctg cagcgaaccg acgatgccgt gaacctcgat cgtctcaccg ggctccggcc
      961 cgagacaatc acggctgcgg tcctcacccc tggtctggga gctcgaggcg ccccgcacca
     1021 ccggcagctg gctgacctcg tattcgcgga gaagctcgat cgcctggcga accgtctgat
     1081 cgcagccaac cgcgacgacg tgcgggacgc cgccggatcg ggcatcgatc aggttaccca
     1141 tccgggatgg cgaggagaag cgggagagga atccgttctc gcgcatccag tcgtcgttgt
     1201 agatcttgga aagatacccg cggccgctat ccggcaggag cacgacgatg agcgcatccg
     1261 gatcgtcgag ccgcttcccg agctcgaggg cggcatggac ggcgagcccg caggagccgc
     1321 cgacgaggat tccctcctca cgcgtgacac ggcgcgccat cagcagcgcc tcggtatcgt
     1381 tcacctggat ccactgatcg acgatgttgc gatcgaacga gcccggccag aagtcctcgc
     1441 caacgccctc gaccttgtac tgatgaatga tctgggacgt gtagatcgac ccttcgggat
     1501 cggcgccgac gatccggatt gccgggttct tttccttgag gaagcgcccg gtgccggaga
     1561 tcgttcctcc cgtgccgaca ccggcgacga aatgggtcac cttgccatcg gtctgctccc
     1621 agatctcggg accggtggat tcatagtgcg tacgcggatt ctggggattg aagtactggt
     1681 tgggctggaa tgcgttcggc acttcgcgcg tcaagcgatc ggccaccgag tagtagctct
     1741 ccggtgaatc acgctcgacc gaggtcggtg tcgtcacgac ttcggcgccg taggcacgca
     1801 gcagctgcac cttctcggcg gagaccttgt ccggcatcac gaagatgcag tgatagcctt
     1861 tcaccgccgc cgccatcgcg agaccgacgc ccgtgttgcc ggatgtcggc tcgacgatcg
     1921 tgccgccagg ccgcagccag ccgcgccgct cggcctcctc gatcatgcgg atgccgatgc
     1981 gatccttgac ggagccgccc ggattcatca tctcgagctt ggcatagatg ggcgtgcgaa
     2041 tgccctgggt gatgtgattg aggcggacga tgggggtatc gcccattgcc tcgatgacgt
     2101 tgtcgaacat gcgacggcgc ggcgcagccg tgccgaccgc acccggaggt ccggcgaccg
     2161 aactggacat gagaatggtc cttcctcaac gaatcgcagg gtcacgagcc acgttccgtg
     2221 caatggaaac gaggtggttc gggggtccgg cccttcacca tcccgggcgc cgaacgatcg
     2281 gcgccgtctt tccaatactt cccggatcga gttccgcttc catgcggaca gctattacgc
     2341 ttcacatttt acagtattga cctgggaagc agcaccgcgc ggacgcggcc cgactcctgt
     2401 tccctcatct ca
//
LOCUS       DASTMD010000042         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_896330, whole genome shotgun sequence.
ACCESSION   DASTMD010000042 DASTMD010000000
VERSION     DASTMD010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896330"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="VFQ54_00630"
     CDS             complement(<1..1278)
                     /locus_tag="VFQ54_00630"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU0163511.1"
                     /translation="MVELANKQILIDGVPRLLISGEIHYFRLDRDDWEDRILKLKETG
                     ANAVASYIPWLCHELEDGSFDLDGRTRAALDLGAFIDLVAKHDLSFIARPGPFIMAEM
                     KNEGIPYRIYEEHPEILPVGWDGQPASTRTVDYLAPAFLAEARRWYEAVIPAIARRLQ
                     PDGGNVIAVQLDNEVGMLSWVSNGPDLTEHVIDDFWNWLGDRRGGYPFAAGDPARRYR
                     AIRSPEPEWSARLMRDLGHYMRDRFARYIATLRAYAEEFGIHDVPFIVNIHGTEAGGG
                     APFPIGISQLYESYTQESGYIGASDHYLGNLTAGNAPDWYLMNALMEAVNRPGQPLAS
                     VEFEAGSGDYGQSMGSRLDPSALDFKLRMAIAQGNRLVNYYLFTGGINYRLDRETGDG
                     NDRISFTGERHGTSAPVNPEGELNYTYPRLARAT"
     gene            1479..>2023
                     /locus_tag="VFQ54_00635"
     CDS             1479..>2023
                     /locus_tag="VFQ54_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0163512.1"
                     /translation="MGKNGPTLHEVAKAAGVSIATVSRVARGLDDVTPATRDRVLRTI
                     QEMDYRASHFGRALVRRSHDTLGIVFPGLRGPYYSEVIHGFEVEAVHARKSVLILGTE
                     LIGHANDQVRGMTDRADGLVIMGGSSIDDGTIQRLVARGVPIVLLARSPLAGIPAVRV
                     DNLGATFELTRHLLEDHGYRRL"
BASE COUNT          372 a          629 c          641 g          381 t
ORIGIN      
        1 cgtcgcgcgc gccaggcggg ggtacgtgta gttaagctcg ccctccggat tcacgggcgc
       61 cgacgtgcca tgacgttcac ccgtgaagct gatccgatca ttcccgtcgc cggtctcgcg
      121 atcgaggcgg tagttgatgc caccggtgaa gaggtaatag ttgaccagcc ggttgccctg
      181 cgcgatcgcc atccgcagct tgaagtccag cgcggacggg tcgaggcgcg aacccatact
      241 ctggccatag tcgccagatc cggcctcgaa ctcgacggag gcgagcggct gccccggtcg
      301 attcacggct tccatgagcg cgttcatcag gtaccagtcc ggcgcgttgc cagcggtcag
      361 gttgcccagg taatgatcgc tggcgccaat gtatccggat tcctgcgtat agctctcgta
      421 gagctggctg attccgatcg ggaagggagc gccaccgccg gcctccgtgc cgtggatatt
      481 gacgatgaag ggcacatcat gaataccgaa ttcctcggca tatgcacgca gcgtcgcgat
      541 gtagcgagcg aaccggtcgc gcatgtagtg gccgaggtcc cgcatcagcc gcgctgacca
      601 ctcgggctcc ggcgaacgga tcgcgcggta tcgccgtgcc ggatcgcccg cggcgaacgg
      661 gtaccccccg cgacgatcgc cgagccagtt ccagaagtca tcgatcacgt gctcggtgag
      721 gtcgggacca ttgctgaccc aggagagcat cccgacctcg ttgtcgagtt gcacggcgat
      781 cacgtttcca ccgtcgggct ggagtcgccg cgcaatcgca ggaatgaccg cctcgtacca
      841 gcggcgcgcc tcggcgagga aagcaggtgc caggtagtcg accgttcgcg tcgaggccgg
      901 ctgaccgtcc cagcccaccg ggagaatctc tggatgctcc tcgtagatcc ggtacgggat
      961 tccctcgttc ttcatctcgg ccatgatgaa cggaccggga cgggcgatga aggagaggtc
     1021 gtgtttcgca acgagatcga tgaatgcgcc caggtcgagc gcggcccgcg tccgcccgtc
     1081 cagatcgaac gatccgtcct cgagctcgtg gcaaagccag ggaatatacg aggcgacggc
     1141 gtttgccccg gtctccttca gcttgagaat ccgatcctcc caatcatcgc gatcgagacg
     1201 aaagtagtgg atctcgccgg agatcaggag gcggggtacc ccgtcgatca gaatctgctt
     1261 gttcgcaagt tcgaccatcg ttgtcgatcc tttctgcacg ccgcccggcg ggatttcgcg
     1321 ggcatcatgc cgttcaggcg tcgcactcca caggggcatc gcccatcagg cgcaccctgg
     1381 tcgggatgac aattgcggca cctgttgcga agtcgtgacc acagtattat gcttgtcgca
     1441 cagtttgtaa acgtttacga gatgatagcg agcgagcaat gggcaagaat ggcccaacat
     1501 tgcacgaggt cgcgaaggcg gccggggtct cgattgccac cgtttcccgc gtcgcgcgtg
     1561 gtctcgatga cgtcactcca gcgacgcgcg atcgtgtgct gaggacgatt caggagatgg
     1621 actaccgggc gagtcacttc ggacgcgcac tcgtgcggcg aagtcacgac acgctcggaa
     1681 tcgtcttccc gggtctgcgc ggaccgtatt actccgaggt tatccacggc ttcgaagtcg
     1741 aggctgttca cgcccgcaag agcgtgctca tcctcgggac ggagctgatc ggccatgcca
     1801 acgatcaggt ccgtggaatg acggacaggg ccgacggcct cgtgatcatg ggcggctcct
     1861 ccatcgatga tgggacgatt caacgcctcg tggcacgcgg cgtgcccatc gtactgttgg
     1921 caaggagccc gctcgccgga attcccgcgg ttcgcgtcga taatctcggc gcgacctttg
     1981 aactcacgcg tcatctcctc gaagaccatg gctatcgaag gct
//
LOCUS       DASTMD010000043         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_985166, whole genome shotgun sequence.
ACCESSION   DASTMD010000043 DASTMD010000000
VERSION     DASTMD010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985166"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VFQ54_00640"
     CDS             complement(<1..1472)
                     /locus_tag="VFQ54_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU0163513.1"
                     /translation="MKRGFDTWGFQGAVFRRVFGLVAFGALAVALVLTQIGFSAISTS
                     WIAAPDVAAQTPSEQTAPLPDAGSVIVVLKPQSGFRTASVAASNDITPTAVYGAAIDG
                     FAADLSDTQIADLSKNPDVAYIVPNIPFQAAAQTLSTAVVRSGLDQSPLVGIDGGGTP
                     VNVDIAIVDTGINLNSSDLDVVGGVNCMTGNPVESGYADDNGHGSHVAGIAAARDDGS
                     GVVGSAPGARLWAVKVLDNNGNGTLDSVLCGLDWVAANMPQNSVVNMSLTATGGDSTC
                     TTGNDALHTAVCSITSEGIPVVVAAGNGTGNNSVGTSTSPIIPAMYPEVISVAAINDY
                     NGQPGGGAATTCTNSWGPDDTLATYSNYGALADIAAPGTCIQSDWKDGSLQYDSGTSM
                     ASPLVAGIAADYKAQNPGASAATVQSWLLSSAASQPMSSSVGYSGHNGGPILYLGGTN
                     ATYPTGDVNPAFAGAKATIVTSIGSSNGSGSGRVWDNDTNT"
     gene            complement(1773..2366)
                     /locus_tag="VFQ54_00645"
     CDS             complement(1773..2366)
                     /locus_tag="VFQ54_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163514.1"
                     /translation="MHARFPSEITACDTSVTISVDFTNVTPATEWAKAGVLVNHGTGE
                     PEQRDIPENHNLPDGSGTWTIVLAPTDLSDATSIFVTVQLYSHDVSGIYINIDEAFDS
                     IDVNPDCGIPDPTSTLAPQPTVPAGATPVVTAPPVTDPGGTVANPGGGKAAVTSLPNT
                     GTGNGTSSIAPILLLVAAIAIIAVAGAMRVGAGARRR"
     gene            complement(2927..>3496)
                     /locus_tag="VFQ54_00650"
     CDS             complement(2927..>3496)
                     /locus_tag="VFQ54_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163515.1"
                     /translation="LSSWNTLPVTEIWLSEDGQTWWNATRVDGSQLHPDQRYDIGLGY
                     WTRYVRLVVPYADQTGLSEIGGIRQLDVWATADGNAQTLDVLGPPVTPIPVPTEVPAP
                     TATLEPTAIPTDVPVPTDVPTDVPLPTDVPTEIPVPTDVPTEIPVPTDIPTDVPAPTI
                     EPTIDAPVPTDPPADTGTDAPGAEETPAA"
BASE COUNT          597 a         1161 c         1071 g          667 t
ORIGIN      
        1 gtgttggtgt cgttgtccca gacgcgaccg gagccgctgc cgttgctgct gccgatactc
       61 gtgacgatcg tggcctttgc cccggcgaag gcaggattga cgtcaccggt gggataggtg
      121 gcgttggtgc ctcccaggta aaggatcggt ccgccattgt gcccgctgta gccgacggag
      181 gaactcatcg gctgcgaggc ggcggagctc aggagccacg attgcaccgt ggcagcagat
      241 gcacccggat tctgcgcctt gtagtcggcg gcgatgccgg cgacgagcgg ggacgccatg
      301 ctcgttccac tgtcatactg aagcgatcca tccttccagt cggactggat gcaggttccc
      361 ggcgccgcga tgtcggcgag cgcgccgtaa ttgctgtagg tcgccagcgt atcgtccgga
      421 ccccagctgt tcgtgcacgt cgtcgcagcg ccaccgcctg gctgaccgtt gtagtcattg
      481 attgcggcga cggaaatcac ttccgggtac atcgcgggaa tgatcggcga tgtgctggtg
      541 ccaaccgaat tgtttccggt tccgttgccg gctgcgacaa cgacgggaat gccctcgctt
      601 gtgatgctgc agacggcggt gtggagcgcg tcgttccccg tcgtacaggt cgagtcgccg
      661 cctgttgcgg tcaggctcat gttgacgacg ctgttctgcg gcatgttcgc cgcgacccag
      721 tccaggccac agaggaccga gtccagagtg ccatttccat tgttatcgag caccttgacc
      781 gcccagaggc gcgcgccagg tgccgagccc acgacgccac tgccgtcgtc ccgcgccgcc
      841 gcgatgcccg cgacgtggga accgtgacca ttgtcgtcgg cgtatccgct ctcgaccgga
      901 ttgcccgtca tgcagttcac accgccgacc acatcaagat ccgacgagtt gagattgatc
      961 ccggtatcga cgatcgcgat gtccacgttg acgggagtac cgccgccatc gatcccgacc
     1021 agcgggctct gatcgagccc ggatcgcacg accgcggtcg acagcgtctg cgccgcggcc
     1081 tggaacggga tgttcggcac gatgtaggca acatcggggt tcttcgagag atcggcgatc
     1141 tgcgtgtcgc tgagatccgc ggcgaagcca tcgatcgcgg cgccgtagac ggccgtcggc
     1201 gtgatgtcgt tcgacgccgc gacgctcgcg gttcggaaac cactctgcgg tttgaggacg
     1261 acgatcaccg aaccggcatc aggcagtggg gccgtctgct cgctcggtgt ctgcgccgcg
     1321 acatccggcg ccgcgatcca gctcgtggag atcgcggaaa atccgatctg ggtcagaacc
     1381 agggcgacag cgagcgcccc gaacgcgacc aatccgaata cacgccggaa gacggcgcct
     1441 tggaaacccc aggtgtcaaa tccgcgtttc atgatttctc ccagaagaga tgagagccca
     1501 ccgccgcggc ccccggcacg aacgccgggc gggcgctgtg agacgctgtc gtgcacaatc
     1561 ggaactatag atgccgacga caccggtggc gatgattatt caagcaaata tggtcccaat
     1621 accgtcgacg ccgccgggat gccgcgatct ccccggcaca cccccgggcg agtcggccac
     1681 cgcatgccgc cggaaacgat ggagggcgcg acgcccgcat cgctgcgaac gccgcgccct
     1741 gttcggggtg atgtcactcc cggtcgagcg ccctatctgc gacgcgcccc tgccccgacc
     1801 cgcatcgcgc cggcgaccgc gatgattgcg atcgcggcaa cgagcaggag gatcggcgcg
     1861 atcgacgacg tcccgttgcc ggttcccgta ttcggcaacg atgtcacggc ggccttcccg
     1921 ccacccggat tcgcgacggt tcctcccgga tccgtgaccg gtggcgccgt gacgaccggt
     1981 gtcgccccgg cggggaccgt cggctgtggc gcgagggttg aggtcgggtc aggaatgcca
     2041 cagtccggat tcacatcgat actatcgaaa gcctcgtcaa tgttgatata gataccactc
     2101 acgtcatgcg agtagagttg cacggtcacg aagatggatg tggcgtcgct aagatctgtc
     2161 ggggcgagca cgatcgtcca ggtaccactc ccatcgggga gattgtgatt ctctggtatg
     2221 tctcgctgtt ccggttcgcc ggttccatgg tttacgagga cgccagcctt tgcccactcc
     2281 gtcgccggcg tcacgttcgt gaagtcgacg ctgatcgtca ccgatgtatc gcatgccgta
     2341 atttcggacg ggaatcgtgc gtgcatcgtc tccgagtcct gcgcagatgc aacgccctca
     2401 tcgactccga ttgccagccc gatcatcaca cccagacaca tgatcgatcg cagaagccgc
     2461 ctggacatca acgcgtccct tctgtcgggt tgcggctgat ccgcaacgaa gccgctcgaa
     2521 aaccgggagc gggaatcctg ctggcaatgg ctccgccact cgcatattcc gcgcagagta
     2581 gccagcgcat ttccccactc cgtctatttc tgtcgccaat ggccctcgaa ttcatgatat
     2641 cgaccagaat ccggaatgca ataggtctcc tctgatatcg ttacgtatat gagcattacc
     2701 gatacatcat cttttctatc gaaatgctta ttccataaag aatctttgct tatacagtgt
     2761 cgaccgcgtt ccgtgcccct gcgcttatcg ggtgcctctc gcaggaaacg acggcatttc
     2821 ccgcgagctt tccgtgtacc gcgcgtcagg cgttcgcacc acaaagaggc gcgacgcccg
     2881 catcactgcg aacgccgcgc ctctcccgga tgccatctct ccatgtctac gcggcgggtg
     2941 tctcctcggc ccccggagcg tcggttcccg tgtccgcggg cggatcggtg ggaaccggcg
     3001 cgtctatcgt cggctcgatg gttggcgccg ggacatcagt cgggatatct gtcggcaccg
     3061 gaatctccgt cggcacatcg gtcggcaccg gaatctccgt cggcacatcg gtcggcagcg
     3121 ggacatcggt cggaacgtcc gtcggtactg gcacgtccgt ggggatcgcg gtcggctcca
     3181 gcgtcgccgt cggggccggc acctccgtcg gtaccgggat cggcgtcacc ggcggtccta
     3241 gcacatccag cgtctgcgcg ttcccgtcgg ccgtcgccca gacgtccagc tgccggatcc
     3301 ccccgatctc gctcaacccc gtctggtccg catacggcac caccagcctg acgtaccgcg
     3361 tccagtaccc cagcccaatg tcatagcgct ggtccgggtg cagctggctc ccatccaccc
     3421 tcgtcgcgtt ccaccacgtc tgcccatcct ccgacagcca gatctccgtc accggcaacg
     3481 tgttccagct gctcag
//
LOCUS       DASTMD010000044         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2057336, whole genome shotgun sequence.
ACCESSION   DASTMD010000044 DASTMD010000000
VERSION     DASTMD010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057336"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFQ54_00655"
     CDS             complement(<1..617)
                     /locus_tag="VFQ54_00655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0163516.1"
                     /translation="MKRTDLSRRSQGQPKSDGFWFVGVLAIGMAVRYAIAPFASGFGY
                     DIQSFVDWARILVNEPFKRFYAEAQSPDHLPGDLWFLWAATHLFQFFGGSNLEGTTFV
                     VVIKSISILGDACIGILIYATVRQLRSERAALVAAALYLLNPASIFMSAVWGQWDSVS
                     VAMVLFALWWFLKADDRWLIGVPFLAWALMIKPQLALLAPFLLAIP"
     gene            complement(968..1414)
                     /locus_tag="VFQ54_00660"
     CDS             complement(968..1414)
                     /locus_tag="VFQ54_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEU0163517.1"
                     /translation="MAQSRNVTTVWTGDIGSGSGVLTAGTGAFSGLGISAATRFGEPN
                     GSTSPEELVAGAHSGCYAMNLAATLTNNGTPPESINANSVVTVSPKAGGGFHLTSALL
                     TVEAKVPGLTDEQFQALAKTAEETCPISSALRGNLEITLDATLLAE"
     gene            complement(1541..>2129)
                     /locus_tag="VFQ54_00665"
     CDS             complement(1541..>2129)
                     /locus_tag="VFQ54_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0163518.1"
                     /translation="AIVDRKIVAELTERLGERINALTVGDPLEDATNIGPLISAEAAA
                     TVFGEIERARGEGVQLVAGGTRPEVNGGHFVAPTLFADVANESRLGQVELFGPVLGVI
                     PVDGLDEAIAVANNVKYGLSASLFTRDLGKAMKFVRGIQAGIVHVNSETAGAEPQVPF
                     GGYKGSSSYSREQGKAARDFYTQIKTVYLDPPPTE"
BASE COUNT          419 a          700 c          639 g          371 t
ORIGIN      
        1 ggaatggcga gcagaaacgg ggcgagcagc gcgagttgcg gcttgatcat cagtgcccag
       61 gcgaggaacg ggacgccgat cagccaccgg tcatccgcct tcaggaacca ccagagcgcg
      121 aagagcacca tcgcgaccga gaccgaatcc cactgtcccc agaccgccga catgaaaatg
      181 ctcgccggat tcaggagata gagggccgcg gcgacgagtg ctgcccgctc cgaacggagc
      241 tggcgcacgg tggcatagat caggatgccg atacaggcat caccaaggat cgagatgctc
      301 ttgatgacga cgacgaatgt cgtcccttcg agattggagc cgccgaagaa ctggaagaga
      361 tgggtcgccg cccagagaaa ccagagatcg cccggcagat ggtcgggaga ctgggcctcg
      421 gcatagaagc gcttgaacgg ctcgttgacg agaatccgcg cccaatccac gaacgactgg
      481 atgtcgtacc cgaagccgct cgcaaacgga gcgatcgcat agcggacggc cattccgatc
      541 gccagcacgc cgacgaacca gaatccgtca gattttggct gcccttgaga tcgtctcgat
      601 aagtcggttc gcttcactcg ctgcacgaat ccatccgctt tggagccgtt gactcgtctc
      661 gaagtactcc tcgaaccccc gagtcacctc tgccaatgat agtcgagcag cttgccgatc
      721 aaactcacga gaaagtgtcc cgtcaactca tgaggcttgt atcgggcagc cccgtctccc
      781 tgactatcgt cacgccttat tccagggctc ttcgagaacc gggccagtct cgggaatgtc
      841 gatgacgtgc cacacgacaa aacccggggg atgcggcttt gcacttcgcc gcatcccccg
      901 ggtttcacca tatcttaacg aacctccgga tcgcggcaac caccgctgac ccgaaggtgc
      961 caccgttcta ctcggcgagt agcgtcgcat cgagcgtgat ctcgagattg ccacgcaacg
     1021 cgctcgagat cgggcaggtc tcctcggccg tcttcgcaag cgcctggaac tgctcgtcgg
     1081 tcaggcccgg aaccttcgcc tccaccgtca ggagcgccga cgtgagatgg aaaccaccac
     1141 ccgccttcgg tgaaaccgtc acgacggaat tcgcattgat cgattccggc ggcgtgccgt
     1201 tgttcgtcag cgtcgccgcg agattcatcg catagcagcc ggaatgcgca cccgcgacga
     1261 gctcctcggg actggtcgat ccattcggct cgccgaagcg ggttgccgcc gagatgccga
     1321 ggccgctgaa ggcgccggtg ccggcggtca ggacaccgga tccgctgccg atatcgccgg
     1381 tccagaccgt ggtgacgtta cgagactgtg ccattgtgtc ttctcctcat ggatgaacgg
     1441 cgcgagaaac accgtcgaaa catcgcgatt cgtttcgcgg gactgccttc agtgtaccgt
     1501 ccgttacggg attgtttatc aacgcggtcg attcccgccc tcactcggtc ggcggcggat
     1561 cgagatagac cgtcttgatc tgggtgtaga aatcgcgcgc cgccttcccc tgctcgcggg
     1621 aataggagct cgatcccttg tagccgccga acggaacctg cggctccgcg cccgccgtct
     1681 cggagttgac gtggacgatg cccgcctgga tcccgcgaac gaacttcatc gccttcccga
     1741 gatcgcgcgt gaagagcgac gccgagagcc cgtacttcac gttgttggcg acggcgatcg
     1801 cctcgtcgag accatcgacg gggatcactc ccagcaccgg accgaagagt tccacctgcc
     1861 cgagacgcga ctcgttggcg acgtccgcga agagcgtcgg cgccacgaag tggccgccgt
     1921 tgacctccgg acgtgtgccg ccggccacca gctggacgcc ctcgccacgc gcccgttcga
     1981 tctcgccgaa gacggttgcg gccgcctccg ccgaaatgag cggaccgatg ttcgtcgcat
     2041 cctccagcgg atcgccgaca gtcagggcgt tgatccgctc gccaaggcgc tcggtcagct
     2101 ccgcgacgat cttgcgatcg acgatcgca
//
LOCUS       DASTMD010000045         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1075019, whole genome shotgun sequence.
ACCESSION   DASTMD010000045 DASTMD010000000
VERSION     DASTMD010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075019"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VFQ54_00670"
     CDS             complement(<1..1136)
                     /locus_tag="VFQ54_00670"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEU0163519.1"
                     /translation="MLTLLDPDTFVELDHLVRHRANQFGMERSRPFGDGLITGFGEID
                     GRKVAVFSQDFTIYGGSLGEAYAEKMVKLMDLAERYGCPIIGINDAAGARIQEGVEGL
                     AGYGDVFFRNVRVSGVVPQISIIAGPCAGGAVYSPAMTDFVFMVEGIGQMFITGPDVI
                     KTVTGETVTREDLGGTSTHMHKSGVAHFAATDEDDLNEQVRYLLSFLPSNNLDDPPRV
                     EAEDDPRRDVPELDEIVPEVSSKPYDMHEVLSAVLDDGEFFEVQRDFGPNLLCGFGRL
                     DGHSIGIVANQPRVLAGTLDIAASEKGARFVRTCDAFNIPLVTFVDVPGFLPGTEQEY
                     NGIIRRGSKLLYAYAEATVPKVTIITRKAYGGAYVVMCSKHIGA"
     gene            complement(1140..1301)
                     /locus_tag="VFQ54_00675"
     CDS             complement(1140..1301)
                     /locus_tag="VFQ54_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163520.1"
                     /translation="MADQATAVIDPEQGHGVEPVSKAQHLADFRDEITAQHAAASEKQ
                     GAKGKRGAM"
     gene            complement(1431..2513)
                     /locus_tag="VFQ54_00680"
     CDS             complement(1431..2513)
                     /locus_tag="VFQ54_00680"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEU0163521.1"
                     /translation="MSARTFRIAVIPGDGIGQEVVPIGLRVLEQLAAQSNGELAFECE
                     SFPWGCEYYLATGRMMDEDGLARLEGFDAIYFGAVGWPGVPDHISLWGLRLAICQGFD
                     QYACVRPVQLLPGISSPLRNASEETIDWVVVRENSEGEYAGVGGRNLASRGAGAEVAV
                     QAALFTEKGCERIIRYAFDLARTRKRKKVTSVTKSNAQQYGMTLWDDVFARVSTDYPD
                     VETEQWLVDAMAARFVLRPDSLEVVVASNLFGDILSDLGSALAGSMGIAASANIDPER
                     RKPSMFEPVHGSAPDIAGKGIANPIAAIWTVQLMLEHLELSAEAEAVSQAIRATTGSG
                     VLTPDLGGTATTAEVGDAVIAHLQRS"
     gene            2810..3349
                     /locus_tag="VFQ54_00685"
     CDS             2810..3349
                     /locus_tag="VFQ54_00685"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016102133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU0163522.1"
                     /translation="MTLTDIPFEKMDGTTGTLADYAGDVVLVVNVASKCGLTPQYEGL
                     QKLYTEKSGDGLTILGFPANNFGGQEPGTDEEIATFCSTNYDVTFPVLSKISVLGEDQ
                     HPLYAELTQAIPTTEGKEPFRERMRASGNTPTEDPDVLWNFEKFLIAKDGHVAARFAP
                     MVTPDDPALSAAIANELAK"
BASE COUNT          624 a         1128 c         1071 g          616 t
ORIGIN      
        1 tccgcaccga tgtgcttcga acacatcacg acgtaggcgc cgccgtaggc cttgcgggtg
       61 atgatcgtca ccttgggcac ggtcgcctcg gcatacgcgt agagaagttt cgaaccgcgc
      121 cggataatgc cgttgtattc ctgctcggtg cccggaagga acccgggaac atcgacgaag
      181 gtcacgagcg ggatgttgaa cgcgtcgcac gtccgcacga agcgtgcgcc cttttcacta
      241 gcggcgatat cgagcgttcc ggcaagcaca cgcggctgat tggcgacgat cccgatactg
      301 tgaccatcga gacgtccgaa cccgcagagg aggttcggcc cgaaatcacg ctgcacttcg
      361 aagaattcgc cgtcgtcaag cactgccgag agaacctcgt gcatgtcgta tggcttcgag
      421 ctgacctcgg gtacgatctc gtccagttcg ggaacatccc ggcgcggatc gtcctcggct
      481 tcgacccgcg gcggatcatc gagattgttc gacggcagga acgaaagcag gtagcgcacc
      541 tgctcgttca ggtcatcctc atcggtcgcg gcgaaatggg cgacaccgct cttgtgcatg
      601 tgcgtcgagg taccaccgag gtcctcgcgt gtcaccgtct caccggtgac cgtcttgatg
      661 acgtcgggcc ccgtgatgaa catctggccg atgccttcta ccatgaagac gaagtccgtc
      721 atcgccggag agtagacggc gcctcccgcg cagggccccg cgatgatcga gatctgcggg
      781 acgacgcccg agacccggac attacggaag aagacgtcgc cgtatccggc gagtccttcg
      841 actccctcct gaatacgtgc gccagccgcg tcgttgatgc cgatgatcgg gcagccgtag
      901 cgttcggcga gatccatcag cttgaccatc ttctcggcgt aggcctcacc gagcgatccg
      961 ccgtagatcg tgaaatcctg cgagaagacg gcgaccttgc gaccatcgat ctccccgaac
     1021 ccggtgatca acccgtcacc gaagggacgg gagcgctcca tcccgaactg gttcgcgcga
     1081 tgccgaacga ggtgatcgag ttcgacgaag gtatcgggat cgagaagcgt cagcacgcgc
     1141 tacattgctc cacgcttgcc cttcgccccc tgtttctcgg aggcggccgc gtgctgcgcg
     1201 gtgatttcgt cgcgaaagtc cgcgagatgt tgcgccttgg agacgggttc tacgccgtgt
     1261 ccctgttccg gatcgatgac ggccgttgcc tggtctgcca cgtcttgctg cctcccctcc
     1321 gcaccgcggc gcggaggtct caaactcgtc tcggggtcgc gccggttcgc gtgattgtag
     1381 ccggtagtcc ccgtctccga tcgcgtcgcc gcgacgacgc gatcgttacg tcaacttcgc
     1441 tggagatgcg cgatcacggc gtcgccgacc tccgccgtcg tcgcggtgcc gccgagatcc
     1501 ggcgtgagaa cacccgaccc ggtcgtcgcc cggatcgcct ggcttacggc ttccgcctcg
     1561 gcgctcagtt cgagatgctc gagcatgagc tggaccgtcc agatcgccgc gatcggattg
     1621 gcgatgccct tcccggcgat gtccggagcg gaaccatgga cgggctcgaa catgcttggc
     1681 ttgcggcgtt cgggatcgat gttggcgctc gccgcgatcc ccatgctccc ggccaatgcg
     1741 ctgccaagat cggagaggat gtcgccgaag agattcgagg cgacaacgac ctcgagggaa
     1801 tccggtcgca gcacgaaccg cgcggccatc gcgtcgacga gccactgctc cgtctcgacg
     1861 tcgggatagt cggtcgatac gcgggcaaag acatcgtccc agagcgtcat cccatactgc
     1921 tgggcgttgc tcttggtgac gctggtcacc ttcttccgct tccgggtgcg cgcgaggtcg
     1981 aaggcgtagc ggatgatccg ctcgcatccc ttttcggtga agagcgctgc ctggaccgcg
     2041 acctcggcac cggcgccacg gctcgcgagg ttgcgcccgc cgacccccgc gtattcccct
     2101 tccgagttct cgcggacgac gacccagtcg atcgtctcct cggacgcgtt gcggagcgga
     2161 ctgctgatcc cggggagcaa ctggaccgga cgcacgcagg cgtactggtc gaagccctgg
     2221 cagatcgcca ggcgaagccc ccagaggctg atgtgatccg gcacgcccgg ccagccgacg
     2281 gccccgaagt agatcgcgtc gaatccctcg agccgggcga gcccgtcctc gtccatcatg
     2341 cgccccgtcg cgagatagta ctcgcagccc caggggaacg actcgcactc gaaggcaagc
     2401 tccccgttcg actgcgccgc gagttgctcg agcacacgca atccgatggg cacgacttcc
     2461 tgtccgatcc cgtcacccgg gatgacggcg atccgaaacg ttctggctga catgagggcg
     2521 atgaccttcc ttggtggaac atgcggcaaa atgaccccgc cagtatacga gcgcatccgc
     2581 gggttcgaag cttttccctc agtgcgggtc accgatcatc agcttttgat gaaccgtaga
     2641 tgcgatgcct gccatcgcct cgggtcggcg gagctgatcc gtgtccgtgc gtgatcgatc
     2701 gaattcgtgc acgacatcac cctcgggctg gcccgatctc cgcctgtctc gatcatactg
     2761 acggatgatt agtatctcct cgacgtccca acgcagaaag gtcccacgaa tgacgcttac
     2821 cgatatcccg ttcgagaaga tggatggcac aaccggcacc ctggcggact acgcgggtga
     2881 cgtggtcctc gttgtgaacg ttgcctcgaa gtgcgggctc accccgcagt acgagggcct
     2941 gcagaagctc tacaccgaga agagcgggga cgggctcacc atcctcggct tccccgccaa
     3001 taacttcggc ggacaggaac cgggaaccga tgaggagatc gcaaccttct gctcgaccaa
     3061 ttacgacgtc accttccctg tgctctcgaa gatcagcgtc ctcggtgagg accagcatcc
     3121 gctctatgcc gagctgacgc aggcgatccc gactaccgag gggaaggagc cgttccgcga
     3181 gcgaatgcgc gcgtccggca acacgccgac cgaggacccg gatgtgctct ggaacttcga
     3241 aaagttcctg attgccaagg acggccacgt tgccgctcga ttcgcgccga tggtcacgcc
     3301 ggacgacccc gccttgagtg cggcgatcgc gaacgaactc gcgaagtagg actcgcacga
     3361 gccaggctga cgcggccagg accggaacgg cgacaagcgc cgtggggacg gggaggcggg
     3421 ctacggagtg cagcgaccc
//
LOCUS       DASTMD010000046         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2950474, whole genome shotgun sequence.
ACCESSION   DASTMD010000046 DASTMD010000000
VERSION     DASTMD010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2950474"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFQ54_00690"
     CDS             <1..522
                     /locus_tag="VFQ54_00690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0163523.1"
                     /translation="LDILVNNAGMSIRGNFWEVPDADWDYQMNVNVRAPFILSQHAAA
                     HMIEHQLRGRVVNVSTIGVASAHKDGMVYNIGKAGVQAMTKNMSYELGPYGITVNCVA
                     PGAVRDRPGQALHETAATPDATAKRLIPYGRYGRAEDIATAVVFFCLPESEWTTGQTL
                     LIDGAHNSYLNDG"
     gene            579..1040
                     /locus_tag="VFQ54_00695"
     CDS             579..1040
                     /locus_tag="VFQ54_00695"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU0163524.1"
                     /translation="MPGPSNADTSLTIIRNLNAPIVDVYNAWTDPAVMGTWFAPNGYH
                     VEELELDVRAGGRYRTVAAGTQGDRHTTVGEYLEVAPNARLVLSWIHDGDPATGDTDA
                     SRLIVTFRETSPTTTRLTLVHEQIATPSGRDALFASWIECLHKLETIFQVR"
     gene            1171..2388
                     /locus_tag="VFQ54_00700"
     CDS             1171..2388
                     /locus_tag="VFQ54_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0163525.1"
                     /translation="MASATTIAPTTTTPDRAMTREVIATFGLFFGSGVIGGSWFSRIP
                     AVKDHLDADVRTVGFVLLCLGLGSLLVMPFTGLLIERTSARAVCTAGLTGACLVYPLL
                     PFIDNVWAFGLVLLLAGGCYGLWDVSINVHGADVERAYGRTIMSMLHGTWSLGLIVGS
                     GIGALLAGADVSLKIHFPVVMLVLLAGGLFLLREWRPYRTEHTGEDAPGTDQGPHLKV
                     LSLTLIFISLMTMCSTLGEGTASDWMALYLHDDHGFSAGLAASVYTVYTIALTIGRFC
                     GSFTIRHLGRVRALRLSGLVSACGVLVMILSSATPAFYVGSALWGFGLAIVFPTAVTA
                     VGELAGRNAARAISAVATISYGSFLAGPPLIGTVAQESSIGRALWITLVLALGISVLA
                     FTTAPRKGTIQSA"
     gene            2750..>3198
                     /locus_tag="VFQ54_00705"
     CDS             2750..>3198
                     /locus_tag="VFQ54_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163526.1"
                     /translation="MLARIGRWLPGERLLSGVLVAALVLLALNVTAPGRDAQGPTPTS
                     TDANLVAQTTITTETTAVDPTARVDPTAAATAGGAGGTEVATSNPTSPASTQADATEA
                     STTGDLLPKYRILTYYGFPGNPNLGILGEYSPEELLPKLQAQAAAYE"
BASE COUNT          535 a         1137 c          965 g          561 t
ORIGIN      
        1 ctcgacatcc tcgtcaacaa tgccgggatg agtattcgcg gcaacttctg ggaggttccg
       61 gacgccgact gggactacca gatgaacgtc aacgtccgcg cgccgttcat cctcagccag
      121 catgccgccg cccacatgat cgagcaccag cttcgcggtc gcgtcgtcaa tgtcagtacg
      181 atcggtgtcg cgtccgcgca caaggacggc atggtctaca acatcggcaa ggccggggtg
      241 caggcgatga cgaagaacat gtcctacgag ctcggcccat atgggatcac ggtcaactgt
      301 gtcgcccctg gcgcggtgcg cgatcgtccc ggccaggcgc tccacgagac ggcggcgact
      361 ccggacgcga ccgcgaagcg cctgatcccc tacggacgat acgggcgcgc cgaggacatc
      421 gcgacggcgg tcgtcttctt ctgcctgccg gaatcggaat ggacgaccgg gcagacgctc
      481 ctcatcgatg gcgcccacaa ctcgtatctg aacgacggat agagccaccg ccagccccgt
      541 ttctccaatg agtcatcgcg aatcccaggg gtaatgccat gccaggtccg tccaacgccg
      601 atacctcgct caccatcatt cgcaacctga acgccccgat cgtcgacgtc tacaatgcat
      661 ggacagatcc cgcggtcatg ggcacctggt tcgccccgaa cggctaccac gtcgaggaac
      721 tcgagctgga cgtacgcgcc ggcggccgct atcgcaccgt cgccgccggc acgcagggcg
      781 atcgtcacac gacggtcggc gagtatctcg aggtcgcgcc gaacgcgagg ctggtcctca
      841 gctggatcca tgacggcgat cctgcgaccg gggacaccga cgcgtcgcga ctcatcgtga
      901 ccttccgcga gacctctccc acgacgacgc gcctgaccct cgtccacgag cagatcgcga
      961 cgccgtccgg gcgcgatgcc ctcttcgcga gctggatcga gtgcctgcac aagctcgaga
     1021 cgatcttcca ggtccggtag ccgccggaca acgcgatcgt cctcgggaga gttcagcggg
     1081 ccattgcgtt ttcgacctgc ttcggagagt ctggaccatc gatcaattcc gggtcaccaa
     1141 cgcccgcatc cgaaggacat gcacgcccag atggcatcgg ccaccacgat cgctccgacc
     1201 actaccacgc ccgaccgcgc catgacccgc gaagtcatcg caacgttcgg cctcttcttc
     1261 gggagcggcg tgatcggcgg tagctggttc tcgcggattc ccgccgtgaa ggaccatctc
     1321 gacgccgatg tgcgcaccgt cggcttcgtg ctgctctgcc tcggtctcgg gtccctgctg
     1381 gtgatgccct tcaccggact cctgatcgag cgaacgtccg cgcgcgcggt ctgcaccgcc
     1441 ggactcaccg gcgcctgcct ggtctatccg ctgttgccgt tcatcgataa cgtctgggcc
     1501 ttcggtctcg tcctgctcct ggcgggtgga tgctatggac tctgggatgt ctcgatcaac
     1561 gtccacggcg ccgatgtgga gcgggcctac gggcggacga tcatgtcgat gctgcatggc
     1621 acatggagcc tcgggctcat cgtaggatcg gggatcggcg ccctcctcgc cggagccgac
     1681 gtctcgctga agatccactt cccggtggtg atgctggttc tcctcgccgg cggtctgttc
     1741 ctgctgcgcg agtggcgccc ctaccgcacc gagcacacag gtgaggatgc gcccggtacg
     1801 gaccagggac cacacctgaa ggtgctgtcg ctcacgctga tcttcatcag cctgatgacg
     1861 atgtgctcga cgctcggcga aggcaccgcc tccgactgga tggcactcta cctccacgac
     1921 gaccacggat tcagtgccgg tctcgcggca tccgtctaca ccgtctacac catcgccctc
     1981 accatcggcc gcttctgtgg atcgttcacc atccggcacc ttggacgcgt ccgcgcattg
     2041 cgtctctccg gtctggtgtc cgcatgtggc gtcctggtga tgatcctgtc atccgcgaca
     2101 ccagcgttct acgtcggatc ggcgctgtgg ggattcggcc tggcgatcgt cttcccgacg
     2161 gcggtcaccg ccgtgggtga actggccgga cgcaacgctg cccgggccat ctcggccgtg
     2221 gcgacgattt cctacggatc cttcctcgcc ggtccgcccc tgatcggcac ggtcgcgcag
     2281 gaatcgtcga tcggtcgtgc cctctggatc accctggtgc ttgccctcgg catctcggtt
     2341 cttgccttca ccaccgctcc ccggaaaggg acgatccaat ccgcgtagcg gctcatccat
     2401 gacccgtggc acggaaacag tctcgccgga tagcgccgtc gactcctggc gcaatccccc
     2461 ggcccggcga gcgaaaggat gatcgccgta cggccggcgc cttaccgcgc ggctgtaccg
     2521 gtcaccgcga gacacgctcc cgcgactccg gtgcgttccg cccccgggtc actgtcgtgt
     2581 actccctgcg ccccgaatct gcgcagatcg cgccgtatgc ctatacttcc gtgacagaat
     2641 gtcagaattg gccaatcccc gcaggttagg catgcaggac gccccgaagc ccttctcgtc
     2701 tgaatggaac ccccagactc acgccagccc cccgcaaggc gtgcgtggcg tgctggccag
     2761 aatcgggcgc tggctgccgg gcgaacggct cctcagcggc gtgctggtcg ccgccctggt
     2821 tctccttgcc ctgaacgtga ccgcccccgg gcgggacgcg caggggccca ccccgacgag
     2881 cacggacgcg aacctggtag cccagacgac catcacgacc gagacgacag cggtcgaccc
     2941 gacggcacgg gtggatccga cggcagcggc cacggcaggc ggtgccggcg ggaccgaagt
     3001 cgccacgagc aaccccacct ccccggcatc cacccaggct gatgcgacgg aggcgtcgac
     3061 aaccggcgac ttgctgccga agtaccggat ccttacctac tatggtttcc ctggtaaccc
     3121 gaatctaggc attcttggcg agtattcccc cgaagaattg ctgccgaagc tgcaagccca
     3181 ggccgcggcc tacgagaa
//
LOCUS       DASTMD010000047         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1254619, whole genome shotgun sequence.
ACCESSION   DASTMD010000047 DASTMD010000000
VERSION     DASTMD010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254619"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VFQ54_00710"
     CDS             <1..702
                     /locus_tag="VFQ54_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0163527.1"
                     /translation="VMFDELARMTGIGPGSRVLEIGAGTGIATRELARRKYDITAVEP
                     GLAMAAIARSHAPRVHFEIATFEKWSDSYDGEPFDLVFSATAFHWLDRETRYARCARL
                     LKRGGHLATLEYTHVDGGDRPFFAAAQACYVEHVPGTPEGQTLPPWDAQPDTTEIIAS
                     PEFDLVGTRQIYEEIPSTRTGYLALIDTYSPMLALDDEHRNRLYDCLGTLIDRDYDGK
                     IRKAYRHELLVARRH"
     gene            complement(699..1943)
                     /locus_tag="VFQ54_00715"
     CDS             complement(699..1943)
                     /locus_tag="VFQ54_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0163528.1"
                     /translation="MFFPSLARSSFGNLTVAQRVAAVSFFGQLFFFVPVMTPYLRDEG
                     LGLAQIAGLQTMLLWAQLVMEVPTGILADRFGHRRSYQVALGMVVCSELWFAFSHTYP
                     MFLIAQVIAGTAFAFASGSVDAYIYESLDEKDRIQRMQQARGLMGAATNLGSVVAYGT
                     TAFVAASLSHSRMVLVLWMGIAALSIGFGLGMTLREHVVTTIRMARPNSLALMREGFR
                     TIRESHALRKVMLLAILSNAFGAHLLVFYQQYFLETGVSGRWFGLALSLGSLVAVGTQ
                     LHAWRMIPRFGTRWGMILATGIPGVVYLLMALTTQPGLAVILFILQWGAIQLSGPLFS
                     GLYNAEIPGGARATVLSLISGLTTIYVGSMGILLGWLAEISLAWMFGLMGAIVLAGTI
                     VVRLGPPVDDALPAVGEAFEVP"
     gene            2370..2519
                     /locus_tag="VFQ54_00720"
     CDS             2370..2519
                     /locus_tag="VFQ54_00720"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEU0163529.1"
                     /translation="MALRIDPEIESKISAFLEDGRYTDANDVIARALDLLQAQEEARA
                     ELRGT"
     gene            complement(2791..>3327)
                     /locus_tag="VFQ54_00725"
     CDS             complement(2791..>3327)
                     /locus_tag="VFQ54_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0163530.1"
                     /translation="DPVELHRQFGVMFQDYAAYQFSAAENIGVGNIEHIDNIVEVMDA
                     ADRAGASSVIAKLPKGFQTTLGKWFEGGHQLSGGEWQKVALARAFMRDAQILILDEPT
                     AALDARAEYELFSRIKELTTGKMALYISHRFSTARMADRIFLLENGQLKEAGTHDELM
                     LQDGQYAELFELQAASYR"
BASE COUNT          676 a         1081 c         1016 g          554 t
ORIGIN      
        1 gtgatgttcg acgaactcgc ccgcatgacg gggatcggcc ccgggtcccg cgtcctggag
       61 atcggcgccg gcaccggcat cgccacgcgt gaactggcac gccgaaagta cgacatcacg
      121 gcggtcgagc cgggtctggc gatggcggcg attgcccgct cccacgcgcc aagggttcac
      181 ttcgagatcg cgaccttcga gaagtggtcg gattcctacg atggcgaacc cttcgatctc
      241 gtcttctcgg ccaccgcctt tcactggctc gaccgggaga cccgatacgc ccgttgcgcc
      301 cgcctgctga agcggggcgg tcatctcgcc acgcttgagt acacgcacgt ggacggcgga
      361 gatcgcccct tcttcgccgc ggcacaggcg tgctatgtcg aacacgttcc cggcacgccc
      421 gaggggcaga cgctcccgcc gtgggacgcc cagcccgaca ccaccgagat catcgcgagt
      481 ccggagttcg atctcgtcgg gacacgtcag atctacgagg agattccctc gacccgcacc
      541 ggctatctgg ccctcatcga cacctactcc ccgatgctcg cgctcgacga cgagcaccgc
      601 aaccgcctct acgactgcct cggcaccctg atcgatcgtg actacgacgg caagatccgc
      661 aaggcctatc gccacgaact gctcgtcgcc cgccggcatt agggaacctc gaacgcttcc
      721 cccacggcag ggagggcatc gtcgaccggt ggtcccaatc gcacgacgat cgttcccgcc
      781 aggacgatcg cacccatcag cccgaacatc caggccagcg agatctccgc gagccagcca
      841 agcaggatcc ccatcgaccc gacgtagata gtggtcaatc cactgatcag cgagagaacg
      901 gtcgcccgcg cgccaccggg aatctccgcg ttgtagagcc cggaaaagag cggtccgctg
      961 agttgaatcg cgccccactg gaggatgaag agaatcaccg ccagcccggg ctgggtcgtc
     1021 agcgccatca gcagatagac gacgccggga attcccgtcg caaggatcat cccccatcgc
     1081 gtcccgaagc gcgggatcat tcgccacgcg tggagttgcg tccccaccgc caccagcgat
     1141 cccagactca gcgccaaccc gaaccaacga ccggacaccc cggtctccag gaagtactgc
     1201 tggtagaaga caagcagatg cgcaccgaag gcattcgaga gaatcgcgag cagcatcacc
     1261 tttcgcaggg catggctttc gcggatcgtg cggaacccct cgcgcatcaa cgcgagcgaa
     1321 ttcggtctcg ccatccggat cgtcgtcacc acgtgttcgc ggagggtcat tcccagaccg
     1381 aatccgatgc tgagcgcggc aatccccatc cagagcacga gcaccatccg tgaatgactg
     1441 aggctggcgg ccacgaaggc agtcgtgccg tacgcgacga cgctgccaag gttggtcgcg
     1501 gcgcccatca accctcgcgc ctgctgcatt cgctggatcc gatccttctc gtccagcgat
     1561 tcatagatgt aggcatcgac gctgccggag gcaaacgcga acgcggtccc cgcgatcacc
     1621 tgggcgatga gaaacatggg ataggtgtgg gagaaggcaa accagagctc cgaacagacg
     1681 accattccga gggcaacctg atagctgcgc cggtgtccga agcgatcggc gaggatgccg
     1741 gtcggcacct ccatcaccag ttgcgcccag agcagcatcg tctggagacc cgcgatctgg
     1801 gcgagaccga ggccctcatc gcgcaggtag ggcgtcatca ccggcacgaa gaagaagagt
     1861 tggccgaaga aggagacggc ggcgacacgt tgggcgaccg tcaggttccc gaaggatgat
     1921 cgcgcaaggg aagggaaaaa catggggaac ggaatcctcg tgatgcgacg gggatccccc
     1981 agggggcgag atcgggagca cgcgcgagtc cgtgagtcga tgtcgatcga ccgtggaggc
     2041 aggcgcggaa caacaaaaat cgtcgtgggg gagccccacg acgacagacc aggagagacg
     2101 gcattgatac gcggaacgtt gcggaatccg ccggagaccg gcatcagtcc gcacccctgg
     2161 cagggtggag ctgcttagaa ggggacgcct gttgttccgc cgaaacctgc cattgcactt
     2221 ctccgtgatg attgttccac tcgttcacgc ttcctccagc gaggaagcga cgtcacgatc
     2281 ctaccacacc gcaggatgat ccagtcattc cgctcgatgc tgtgctcaag gcaaccggga
     2341 tgttaggatc ttggtaagga ggcatgcaca tggcgctacg aatcgaccct gaaatcgaat
     2401 ccaaaatttc agcctttctg gaagatggtc gctacacgga tgcaaacgat gtgatcgcca
     2461 gggcccttga cctccttcag gcgcaggagg aagctcgtgc tgagctccgc gggacataga
     2521 tctcggggtc gagcagattc ggcggggaga agggatcgag gtcgacgacg aataccggga
     2581 caacctcgac cgcgaaatcg gtgtggccct cggccacggg tcagcactag gctgacccct
     2641 acgaaaacca ttaaccacgc cattccgtag gggcgaccct ggtggtcgcc cggtgccaca
     2701 cccgcagaag ttgcttcacc cgccagcact tccggcgggt cttgccaacg tctcgtgacg
     2761 attcgattca ttcctgattt agtttgaagg ttatcggtag ctggcggcct ggagctcgaa
     2821 gagttccgcg tattggccgt cctgcagcat cagttcgtcg tgggtgccgg cttccttcaa
     2881 ctggccgttc tccaggagga agattcggtc cgccatgcgc gccgtcgaga aacggtgcga
     2941 gatgtagagc gccatcttcc cggtcgtcag ttccttgatc cggctgaaga gctcatactc
     3001 cgcacgcgcg tcgagcgccg cggtcggctc gtccaggatc aggatctggg cgtcgcgcat
     3061 gaaggcgcgt gccagcgcca ccttctgcca ctcgccaccc gagagctgat gaccgccctc
     3121 gaaccacttg cccagcgtcg tctggaatcc cttcggcagc ttcgcgatca cactcgaggc
     3181 gccggcccgg tcggccgcat ccatcacttc gacgatgttg tcgatgtgtt cgatgttgcc
     3241 gacgccgatg ttctcggcgg cggagaactg ataggcggcg taatcctgga acatcacgcc
     3301 gaactggcgg tgcagctcga ccggatc
//
LOCUS       DASTMD010000048         1551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_361423, whole genome shotgun sequence.
ACCESSION   DASTMD010000048 DASTMD010000000
VERSION     DASTMD010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361423"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..1344
                     /locus_tag="VFQ54_00730"
     CDS             316..1344
                     /locus_tag="VFQ54_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0163531.1"
                     /translation="MKTTTLGYSDLVASRIALGCAKFGSEIRPCDAFDLIDLFVERGG
                     NLIDVPNGQSEFAVGSWLQRRSRADVMIVATGHFSGEPLSLTRSYLDTALDASLRVLG
                     TDYIDLYQVEGPDHQHGIEEVARFLEHAQASGKIRHAGTSRLTIVDLVCLDGATRALG
                     ISPGVSFQSASGYNLLERRIESDILPVALTLGLGVIARGPLGSDWLTGRHRREQYLPA
                     DIDSSELPRPDADPWSSRNIERTWRILDELGNVARETGLPPANVALAWQLSRSGVATA
                     IVEPRNSGQLAGLIPAAGIELDARHLDRLTRVSEPPASRHETGWLNQLAIRERREPEL
                     AGSGHRPA"
     gene            complement(1329..>1551)
                     /locus_tag="VFQ54_00735"
     CDS             complement(1329..>1551)
                     /locus_tag="VFQ54_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163532.1"
                     /translation="DEVGANRSLNLRWTPAKELEPVAADSTAAVSMTRKRPRRVLRWG
                     RFSSSTFHTPWAYALTVASERRYPGQAGR"
BASE COUNT          263 a          502 c          498 g          288 t
ORIGIN      
        1 cacacgagcc ccggccgggt cgatccggtc ggggcttgtg tgtctgtcgt ccggcaccca
       61 aacggatgac gtattgcgca atcgactacc tagccggggt agacacgaat ggtcacttcc
      121 taccgatgaa tgcgaacgtc ctggccgcca tcatcgtcct gccagtcgcg gtccgggagg
      181 attcccgtct cgatgcgtac gctctcccgc gcgctggaac tactgggtgg atttcgctcg
      241 acgatgtcca ttgggcgttc gaagcccggg catcgtgcat tccagggagc atgcttttct
      301 cctcgagagg taatcgtgaa gactactacg ttggggtaca gcgacctcgt cgcgtcccga
      361 attgcactcg gttgtgcgaa gtttggctcg gaaatccgtc cctgcgacgc gttcgacctg
      421 atcgatctct tcgttgaacg tggcggcaat ctgatcgatg tcccgaatgg acagagcgag
      481 tttgccgtcg gttcctggct tcagcggcgt tcccgcgcgg atgtgatgat cgtcgccacc
      541 ggacacttct ccggtgagcc gctctcattg acgcggtcat atctcgatac cgcgctcgac
      601 gccagcctgc gcgtcctggg taccgactac atcgatctct accaggtcga aggtccggat
      661 catcaacacg ggatcgagga ggtcgcgcga ttcctcgagc atgcgcaggc gtccggaaag
      721 atcaggcacg cgggcacgtc ccggctcacg atcgtggatc tcgtctgcct ggacggggcg
      781 acgcgtgcgc tcggcatcag cccgggtgtc tcgtttcaat cagcctccgg gtacaaccta
      841 ctcgagcgcc ggatcgagtc ggacattctg cccgtcgcgc tgacactcgg tctcggcgtg
      901 atcgcgcggg gaccgctcgg aagcgactgg ctgaccgggc gacatcggcg cgagcagtat
      961 cttcccgccg atatcgattc cagcgagctg cccaggcccg atgccgaccc gtggagctcg
     1021 cggaacatcg agcgaacgtg gaggattctc gacgagctcg gaaacgtcgc aagggagacc
     1081 ggcttgccgc cggcaaacgt ggcgctcgcg tggcagctga gtcgatccgg agtcgcgacc
     1141 gcgatcgtcg aaccacgcaa ctccgggcaa ctcgccggcc tgatccccgc cgccgggatc
     1201 gagcttgatg cccgccacct ggatcggctg acgcgcgtgt cagaacctcc cgcttctcgc
     1261 cacgaaaccg gatggctgaa ccagctggcg atccgtgagc ggcgggaacc agagctcgcc
     1321 ggctccggtc atcgaccggc ctgacctgga tatcgccttt cgctcgccac ggtgagcgca
     1381 tacgcccacg gcgtgtggaa tgtggacgac gaaaagcgac cccagcgcag aacgcgccgg
     1441 ggccgctttc ttgtcatcga gacagcggcg gtcgagtcgg cggcgacggg ctcgagctcc
     1501 ttcgccgggg tccagcgaag gttgagggag cggttggcgc ccacctcgtc g
//
LOCUS       DASTMD010000049         6224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2146531, whole genome shotgun sequence.
ACCESSION   DASTMD010000049 DASTMD010000000
VERSION     DASTMD010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2146531"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VFQ54_00740"
     CDS             complement(<1..503)
                     /locus_tag="VFQ54_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0163533.1"
                     /translation="MTPENMNVLKFRSTRLARLGCLAQVAILILLFCPWAQASVAAQA
                     TPAAAQAGRIERRWSTPDCADLGSSQFVGSFVICAGDSGIRAYDPATGARAWTFKESR
                     GGSKLPPVVSGASLIQGVGSEVVAVYSASGRQEWSATVAEGAAVNEVRPSSSGSIVFV
                     RTNESIEA"
     gene            complement(744..2384)
                     /locus_tag="VFQ54_00745"
     CDS             complement(744..2384)
                     /locus_tag="VFQ54_00745"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU0163534.1"
                     /translation="MSTIEPSIEIHAEPGRVVTSDIAHEVSERRTFAIISHPDAGKTT
                     LTEKLLLYGGAVQMAGSVTARRTQRHAASDWMAMEQQRGISITSTVLAFPYRGHQLNL
                     LDTPGHQDFSEDTYRTLTAVDSAVMVLDGARGIEPQTLKLFEVCRRQHTPIFTFINKI
                     DRPALHPLELMDEIERKLGIKTYPLNWPIGDGSDFRGVYDRQSHTVHLFERTEHGARQ
                     APVRVADLDDPLLDDQIGAARAQQLREEMELLDLAGTPFDLQEILSGQLTPVCFGSAL
                     TNFGVQLFLDRFIEHAPQPGAHETTRRTMEPTDPTFSGFVFKIQANMDPRHRDRVAYV
                     RIVSGKFERDMDAWHPRLKRKVRLARPMRLFGQDREVVDEAYAGDVVGLINPGTFTIG
                     DTISAEDVGEFAALPRFQPEHFALLRHARADRYKQFQKGLTQIEEEGAIQLFYPVSSG
                     SREPVLAAVGALQFDVVRFRLEAEYSVETIMEPLGYSAARWVSGDEAEITTIGNGRGR
                     MRAEDQNGREVILFTTEWDLRYVEEHAENVTFQSLAGL"
     gene            complement(2499..2912)
                     /locus_tag="VFQ54_00750"
     CDS             complement(2499..2912)
                     /locus_tag="VFQ54_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163535.1"
                     /translation="MRDKDTERQGTGTTNRPASDGPRSSAPDNRADRKQCPWCDSFNT
                     RFIQRGFTGPTDEKDQYIICDDCNRLTYEILSRSNRDMRVSQFQVGNTVRDGLTQTKY
                     VINRVLKVGLNEYLLYVKPITRREAKEDEPGLRFR"
     gene            3173..5914
                     /locus_tag="VFQ54_00755"
     CDS             3173..5914
                     /locus_tag="VFQ54_00755"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU0163536.1"
                     /translation="MRTSFAVEGMTCAACVRRVERALDKIDGVSNVAVNLATEKATIQ
                     LDPTLADAGTLQAAIEKAGYKAGRIAIPVPVPMASPVVVPPAAPVSEGDAEPAVTFDI
                     DGMTCASCVRRVERALAKVDGVASASVNLATERASVAFEPGVVVDTDLLTSAVAKAGY
                     TAHPIVPSLPASTGDGVDVSNIEAEEDARRISRDRDLASMKRKSLWSLATGIAMMVAM
                     YAPLPISERTLAPLLLTAATIVQIWAGRVFYEQTWIAARHRSTNMNTLVAVGTSVAYG
                     YSAFVTLWPQLADRWNLPYHLYYETAVIIIALILLGRWMEARARKATGDAIRALMGLQ
                     AKTARVVRGAGEVDIPLEQVIVGDVVRVRPGEKVPVDGTVVEGVSRVDESMLTGESMP
                     IEKRAGDEVVGATINQTGSFLFRATKVGRDTVLAQIVRLVEEAQGSKAPMQKLADNIA
                     SIFVPIVLGISVLTFGIWAIWGPSPMLSYAIPAVVAVLVIACPCALGLAAPTAIMVGT
                     GKAAELGILVRGGESLEMARSVDTIVLDKTGTITRGKPSVTAIETTGEIEADALLGLV
                     AAAEVGSEHPLGRAIVVEAETRGIGVPAMTGFEAVPGQGIIARVHGKTLAIGNLALMD
                     RVGADATTFMGRADELADRGATPIHVAVDGRIAGLIAVADQAKTESAEAIAELKALGL
                     DVWMVTGDNERTARAVARDVGVTNVLAGVLPSEKSAKVRELQAQGKKVGMVGDGINDA
                     PALAQAELGIAIGTGTDVAMAASDITLIGGDLRSIVSAIALSRRTVQTIRQGLFWAFA
                     YNIVLIPVAMGILYPAWDILLSPVLAAAAMAMSSVSVVTNALRLRGFRRPSSAAVILH
                     PPIRARVREWGYLAGIAVVALAIGAGALWLSDRAGMGVTADERPAADVQPMPMDHGN"
BASE COUNT         1085 a         1887 c         2133 g         1119 t
ORIGIN      
        1 gcttcaatgg actcatttgt ccggacaaag acgatcgatc cagaactgct cggacgcact
       61 tcattgacgg cagcgccctc ggcgacagtc gcagaccatt cctgtcgtcc cgacgcggag
      121 taaactgcga ctacttcgct gccgacaccc tgaatcaacg aagcgccaga taccacaggt
      181 gggagctttg aaccgccccg tgactccttg aacgtccatg ctcgggctcc agtcgccgga
      241 tcgtacgccc gaatcccgga atctcctgcg caaattacaa aggatccaac aaattgcgat
      301 gagccgagat cggcgcaatc gggagtggac cagcggcgtt cgatacgacc agcttgagcg
      361 gctgccgggg tcgcctgcgc tgccacactg gcttgcgccc agggacaaaa aagcaggatg
      421 agaatcgcca cctgggcgag gcaacccagc cgcgcaaggc gagtcgatcg gaatttgagc
      481 acgttcatgt tctccggcgt cattgagctg agcttgctgt cctcagctca ttgtacttgc
      541 cgaagatcaa gctggggaaa gcggttgtac cgaggggcac ccagcatatt cagctgtaac
      601 tccgaagggc aatcgaacga cctagtggcg tatccaccaa gccacgacca gcgtggtcat
      661 cggggaaatg cgcattgaac cagcgtacgt tcgtagggcg ggacttctat gtcccgcctc
      721 cacggtgagt cgacggtgtc ctcttagaga ccggcgaggg actggaaggt gacgttctcg
      781 gcatgttcct cgacgtagcg gaggtcccac tccgtcgtga agaggatcac ctcgcgaccg
      841 ttctggtcct cggcccgcat ccgtccgcga ccattgccga tggtcgtgat ctccgcctcg
      901 tcgccactca cccagcgtgc ggccgagtat ccgagcggct ccatgatcgt ctcgacgctg
      961 tactcggctt ccaggcggaa gcggaccacg tcgaactgca gggcgccgac ggcggcaagc
     1021 accggttcgc ggctgccgct ggagaccggg tagaagagct ggatcgcgcc ctcctcctcg
     1081 atctgcgtca gtcccttctg gaactgcttg tagcggtcgg cccgggcatg gcgaaggagc
     1141 gcgaagtgct ccggctggaa ccggggcagc gccgcgaact cgccgacgtc ctcggcgctg
     1201 atcgtatcgc cgatcgtgaa ggttcccggg ttgatcagtc caaccacatc cccggcatag
     1261 gcttcgtcga cgacctcgcg atcctggccg aagagacgca tcggacgcgc gaggcggacc
     1321 ttgcgcttga ggcggggatg ccaggcatcc atgtcgcgtt cgaacttgcc ggagacgatc
     1381 cgcacgtagg cgacgcggtc gcgatgccgc ggatccatgt tcgcctggat cttgaagacg
     1441 aatccggaga aggtcggatc ggtcggttcc atcgtccggc gcgtcgtctc gtgcgcgccc
     1501 ggctgcggcg cgtgttcgat gaagcgatcg aggaagagct ggacgccgaa gttcgtcaac
     1561 gccgagccga aacagaccgg tgtcaactga ccggagagaa tttcctggag gtcgaacggc
     1621 gttccagcga ggtccagcag ttccatctcc tcgcgaagct gctgggcgcg cgccgcgccg
     1681 atctgatcgt cgagcagcgg gtcgtcgaga tcggcgaccc ggaccggcgc ctgcctcgcg
     1741 ccatgctcgg ttcgctcgaa gagatgaacc gtgtgcgact ggcgatcgta gacgccgcgg
     1801 aagtcgctgc cgtcgccgat cggccagttg agcggatacg tcttgatccc cagcttgcgc
     1861 tcgatctcgt ccatcaactc gagcggatga agcgccggcc ggtcgatctt gttgatgaag
     1921 gtgaagatcg gcgtgtgctg ccggcggcag acctcgaaga gcttgagtgt ctgcggctcg
     1981 atgccgcgag cgccgtcgag caccatcacg gcgctatcga cggcggtcag ggtgcgatag
     2041 gtatcttcgg agaaatcctg gtgaccgggc gtatcgagga gattgagctg gtggccgcgg
     2101 tatggaaagg cgagcaccgt cgacgtgatc gagatgccac gctgctgctc catcgccatc
     2161 cagtcggaag cggcgtggcg ctgcgtgcgg cgtgccgtga ccgagcccgc catctgcacc
     2221 gcgcctccat agagaaggag tttctcggtg agggtcgtct tcccggcgtc cggatgggag
     2281 atgatcgcga aggtgcgtcg ctccgaaacc tcgtgcgcga tgtccgaggt gacgacgcga
     2341 ccgggttcgg catgaatttc gattgatggt tcgatggtgg acatgaaggt acatcctcgg
     2401 gtgtgggtca gacggaaggg cgcgcgaaaa aaccgcgcga tcggggagtc gatctgctgg
     2461 gcgtccgcag gacgcgggga cggtgttagg ttggaaactc agcgaaatcg cagaccgggt
     2521 tcatcttcct tggcctcacg acgcgtgatc ggcttcacgt agagcaagta ctcgttgagg
     2581 ccgaccttga ggacgcgatt gatgacgtat ttcgtctggg tcaggccgtc gcggacggtg
     2641 ttcccgacct ggaactggct gacccgcatg tctcgattgc tgcgggaaag gatctcgtac
     2701 gtcaggcgat tgcaatcatc gcagatgatg tactgatctt tctcatcggt gggaccggtg
     2761 aatccacgct ggatgaatcg ggtgttgaag ctgtcgcacc acgggcactg ttttcgatcc
     2821 gcccggttgt ccggcgcgga tgatcgcggt ccatcggagg caggtcggtt cgttgtgccg
     2881 gttccctgac gctccgtgtc tttgtcccgc atcgtcacag ctaccatcca gaatgcgttc
     2941 gaacctatcc caacggtcgc gtgaccaccg caagtgtacg tcccgcgccg gtggcgtgtc
     3001 atcgcaaagg cacaacattc tctcgattcc gagcgttgat attatacccc accggggtat
     3061 aatggggagc gtgaatatgt ccgtgggcgg cgttcgccct acagccaggg cctaatcgat
     3121 gccgccagtc acccctcccg tatctgaacc agccgtcggc acgagcgggc ttgtgcgaac
     3181 cagcttcgcc gtcgaaggca tgacgtgcgc ggcctgcgtt cgccgcgtcg agcgtgcgct
     3241 cgacaagatt gacggcgtct cgaacgtcgc ggtcaacctg gcgacggaga aggcgaccat
     3301 acagctcgac cccacattgg ccgacgccgg cacgctgcaa gcggcgatcg agaaggcggg
     3361 atacaaggct ggccggatcg cgatcccggt tcccgtcccg atggcatctc cggtggtcgt
     3421 gccacccgcc gcgcccgttt cggagggtga cgcggaaccg gcggtgactt tcgacatcga
     3481 tgggatgacc tgcgcctcct gcgttcgccg ggtcgagcgc gccttggcca aggtcgacgg
     3541 cgttgcctcg gccagcgtca atctggcaac ggaacgggcc agtgtcgcgt ttgagcctgg
     3601 ggtcgtcgtc gacacggacc tgctgacgtc ggcggttgcg aaggctgggt ataccgcgca
     3661 cccgatcgtg ccgtccttgc cagcaagcac gggcgacggg gtggacgtct cgaacatcga
     3721 ggcggaagag gatgcgcggc ggatcagccg cgatcgtgac ctcgcgagta tgaagcggaa
     3781 gtcgctctgg agtctcgcga ccggaatcgc gatgatggtc gcgatgtacg cgccgctgcc
     3841 gatctcggag cggacgctgg cgccgcttct cctgaccgcg gcgacgatcg tccagatctg
     3901 ggcggggcgc gtcttctacg agcagacgtg gatcgccgcg cgtcaccggt cgacgaacat
     3961 gaatacgctg gtcgccgtcg gtacgtccgt cgcctacggc tacagtgcct tcgtcacgct
     4021 ctggccacaa ctggccgatc gctggaatct cccctaccac ctctactatg agaccgcggt
     4081 gatcatcatc gcgctgatcc tgctcgggcg atggatggaa gcgcgcgcca ggaaggcgac
     4141 cggcgacgcg atccgtgccc tgatgggtct ccaggcgaag acggcgcgag tggtccgggg
     4201 agcgggcgag gtcgacattc cgctcgagca ggtgatcgtc ggagatgtcg ttcgcgtgcg
     4261 gccgggcgag aaggttccgg tcgacgggac cgtggtcgag ggcgtctcca gggtcgacga
     4321 gagcatgctg accggcgaga gcatgccgat cgagaagcga gcgggcgacg aggtggttgg
     4381 tgcgacgatc aaccagacgg gttcgttcct cttccgggcg acaaaggtgg gccgcgacac
     4441 ggtcctcgcc cagatcgtcc ggctcgtcga ggaggcgcaa gggtcgaagg cgccgatgca
     4501 gaagctggcg gacaacatcg ccagcatctt cgtcccgatc gtactcggca tctccgtcct
     4561 caccttcgga atctgggcga tctggggccc atcgccgatg ctctcgtacg cgattccggc
     4621 ggtggtcgcc gtactcgtca tcgcctgccc atgcgcgctt gggctggcgg cgcctaccgc
     4681 gatcatggtg ggaacgggga aggcggcgga gcttggtatc ctcgttcggg gtggcgagtc
     4741 gctcgagatg gcgaggtcgg tcgacacgat cgtgctcgac aagacgggca cgatcacccg
     4801 agggaagcca tctgtgaccg cgatcgaaac cacgggcgag atcgaagcgg acgcgctttt
     4861 ggggctcgtc gcggcggcgg aggtgggctc cgagcatccc ctgggtcgcg cgatcgtcgt
     4921 cgaagcggaa acacgtggca tcggggtacc ggcgatgacc ggattcgagg cggttccggg
     4981 tcagggcatc attgccaggg tccacggaaa aacgctggcg atcgggaatc tcgccttgat
     5041 ggatcgcgtc ggtgccgatg ccaccacgtt catgggccga gcagacgagc ttgcggatcg
     5101 tggtgcgacg ccgattcatg tcgcggtcga cggtcgcatc gccggactga tcgcggtcgc
     5161 ggatcaggcg aagacggaat ccgccgaagc aatcgccgag ttgaaggcgc tcgggctcga
     5221 cgtgtggatg gtcaccggcg acaacgagcg gaccgcgcgg gcggtcgcgc gcgacgtcgg
     5281 agtcaccaac gtcctcgcgg gagtactgcc gtccgagaag tcggcgaagg tgcgcgagct
     5341 tcaggcgcag ggcaagaagg tcggcatggt tggcgacggg atcaacgatg cgccggcgct
     5401 cgcccaggcc gagctcggga tcgcgatcgg cacggggacg gacgtcgcga tggcagcgtc
     5461 ggatatcacc ctgatcggtg gcgacctgcg gtcgatcgtc agcgcgatcg cgctctcgcg
     5521 gcggacggtg cagacgatca ggcagggtct cttctgggcc ttcgcctata acatcgtcct
     5581 gattccggtg gcgatgggga tcctctaccc ggcctgggat atcctgctca gcccggtgct
     5641 cgctgccgcc gcgatggcga tgagtagcgt cagcgtggtg accaacgcgc tccgcctccg
     5701 tggcttcagg cgaccatctt cggcggctgt gatcctccat ccgccgatcc gggcgcgagt
     5761 ccgcgagtgg ggatacctcg ccggcatcgc agtcgtcgcg ctggcgatcg gcgccggggc
     5821 gctctggctg agcgaccgcg ccggcatggg cgtcaccgcg gacgagcgtc cggctgccga
     5881 cgtgcagccg atgccgatgg atcacgggaa ctgatctcat ctcaaccgaa gcgaaatccg
     5941 gaacggtcgc aacgtttggt gatctggggt tggcagggcg gggttttata ccccgccgca
     6001 gacgggaacg tctggataac gggccaggta ctcgtgtcgt tctgccacgg acgcgtgtcg
     6061 gcggaaccga cccgaggcgg ccacaagggc cgcgtctacg gttcatccga cgatcgggag
     6121 gggtgagggt tgctcctgcg acggtcatat cgacctgcgg tcgatgggcc acggggtggc
     6181 ccgccacggg atcgtcgtga tcgggaggcg tgagcgtcgc tcgg
//
LOCUS       DASTMD010000050         3986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_808074, whole genome shotgun sequence.
ACCESSION   DASTMD010000050 DASTMD010000000
VERSION     DASTMD010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808074"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VFQ54_00760"
     CDS             complement(<1..716)
                     /locus_tag="VFQ54_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0163537.1"
                     /translation="MTFDPFALNQIGARVGFGTTGISRDDMSDEAAAETFRTAWESGV
                     RYFDTAPMYGSGVAEVRTGRALAGIPRDDYVLSTKVGRLVKGAPDVRSAGTDWYYDFS
                     ADGIKRSIAESLERLQLDRVDILYIHDADDYVDQAVAEAWPVIEQLRSEGVVRAVGAG
                     NTTVPTLLRFARETTMDFFLVAGRYTMLADDAQDELLPLCIEKGIGVVIAQALHGGLI
                     DGVADPQLYYQPTDDATKAKV"
     gene            915..2483
                     /locus_tag="VFQ54_00765"
     CDS             915..2483
                     /locus_tag="VFQ54_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0163538.1"
                     /translation="MEKRWDLIIVGAGSAGAALAARLAERGRRVLLLEAGPDYRSAEM
                     PEAWRSPNPLPAILAPEESGSLVWPDLLSSRTDAQEPRLYWRGRGAGGSSSINGQIAI
                     RPPREDFDDWAALGCPGWGWDEVLPYFCRLENDEAFGSAPYHGDSGPIPIYRMPRERW
                     GAVDNALAMAAMEAGFAWADDLNAPGATGVSPYPINSRDSRRVSTNDGYLEPSRSNPN
                     LTIRGDALVNRILFDGDRAIGVEIVAGDSVVQEYADDVVLSAGVIHSPAILMRSGIGP
                     ADHLRELGIDIVVDLPVGEGMQDHPIAGVELPLRADTAIQTPDDRHTNCCVRYSSNDP
                     DGTPNDMMLVSMNQAVLSMDAAETAAGAGLIGVWLNQAYSRGTVRLTSRDPRVHPEVR
                     ESMLSDERDRRRMREGIRMLAKMAALPEVTSICAFDPAKANGPLLAALDGNDDDLDAF
                     LLANVSDTQHGTSTCRMGAAGDPGAVVDTECRVLGIDGLRVIDASIFPFVLRSNTNLA
                     TIMLGEYMADRLSQ"
     gene            2508..3422
                     /locus_tag="VFQ54_00770"
     CDS             2508..3422
                     /locus_tag="VFQ54_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0163539.1"
                     /translation="MPRVAVAQTGSVLFDTPATLDRMQAICEEAQRERVDLLVFPEAF
                     IGGYPKGTDFGARIGSRTPEGRDDFLRYYQSAIAIPGPETARIGEFAREMSSYLVVGV
                     IERDSGTLYCTALYVGPDGAILGKHRKLMPTAAERLIWGFGDGSTIDVVDSPHGRFAV
                     TICWENYMPALRTAMYAQGIQLWCAPTVDDREIWQASMRHIAYEGRCFVLSACQYMTR
                     ADAPDDYICIQGDDPATVLIRGGSVIVSPLGEVLAGPISNESGLLVAEIDLDDCIRGK
                     YDLDTVGHYARPDIFQLSVDTRPKPPVL"
     gene            complement(3768..>3986)
                     /locus_tag="VFQ54_00775"
     CDS             complement(3768..>3986)
                     /locus_tag="VFQ54_00775"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEU0163540.1"
                     /translation="TTSGKETYEIGRYLEVAATPDGKLVVDFNLSYNPLCAYSSGWSC
                     SIPPVENFLGVPIQAGEILPEFVDHSDH"
BASE COUNT          677 a         1356 c         1280 g          673 t
ORIGIN      
        1 accttcgcct tcgtcgcgtc gtccgtgggc tggtagtaga gctgcggatc ggcgacaccg
       61 tcgatcaggc cgccgtgcag cgcctgcgcg atgacgactc cgattccctt ctcgatgcag
      121 agcggcagca actcgtcctg cgcgtcatcg gcgagcatcg tgtagcgacc ggcgacgagg
      181 aagaagtcca tcgtcgtctc gcgagcgaag cggagcaggg tgggcaccgt cgtgttcccg
      241 gcgccgaccg cacgcacgac accttccgag cgcaactgct cgatcaccgg ccacgcctcc
      301 gcgacggcct ggtcgacgta gtcgtcggca tcgtggatgt agaggatgtc gacgcgatcg
      361 agctggagtc gctcgaggct ttccgcgatc gagcgcttga tgccgtccgc gctgaagtcg
      421 tagtaccagt cagtgcccgc cgagcggaca tccggcgcac ccttcacgag acgcccgacc
      481 ttcgtcgaga ggacgtagtc gtcacgcgga attcccgcca gggcgcgccc agtccgaacc
      541 tcggcgaccc cgctgccata catcggggcg gtgtcgaagt agcggacgcc gctttcccag
      601 gcggtgcgga aggtctcggc ggcggcttcg tcgctcatgt catcgcggga gatccccgtc
      661 gttccgaaac ccacgcgggc gccgatctgg ttcaatgcaa acggatcgaa ggtcatggtt
      721 ttcccatcat tcatgcgttg cgcacggagc gccggttcaa tcggtatcac ttgatactac
      781 tcaggattcg actaccggaa cagcagatca ccatgccaac gacctggcgg tcccgatccg
      841 attgccgaac gcctcgaatc accggagaat ccagggcgac gtgacattcc gtggcacaag
      901 tggaggcagg atcgatggag aagcgctggg atctgatcat cgtcggggcg ggctcggccg
      961 gggccgccct cgccgcgcgc ctggccgaac gcggccgccg cgtcctgctc ctcgaggcag
     1021 gcccggatta ccgctcggcg gagatgccgg aggcatggcg ctcgccgaat ccgctcccgg
     1081 cgatcctcgc gccagaggaa tcgggcagcc tggtctggcc cgatctcctc tcgagccgta
     1141 ccgacgccca ggaaccgcga ctctactggc gcggccgtgg cgccggtggc agttcctcca
     1201 tcaacggcca gatcgcgatt cgccccccgc gagaggattt cgacgactgg gcggcgctcg
     1261 gctgcccggg ctggggatgg gacgaggtgc tcccctactt ctgccgcctc gagaacgacg
     1321 aggccttcgg ctcggcacca tatcacggcg attcgggtcc gatcccgatc taccggatgc
     1381 cgcgcgagcg ctggggcgcg gtcgacaatg cactcgcgat ggcggccatg gaagcgggat
     1441 tcgcctgggc ggacgatctc aacgcacccg gcgcgacggg cgtctcgccc tacccgatca
     1501 actcgcgcga ttcgcgtcgt gtctccacga acgacggcta tctcgagccg tcgcgatcga
     1561 acccgaacct gacgattcgc ggcgacgccc tcgtcaaccg catcctgttc gacggcgatc
     1621 gcgcgatcgg ggtcgagatc gtcgccggcg attcggtcgt gcaggaatac gcggacgatg
     1681 tcgtgctcag cgccggagtg atccattccc cggcgatcct gatgcgatcg gggatcggac
     1741 cggcggacca tcttcgcgag cttgggatcg acatcgtcgt cgatctcccg gtcggtgagg
     1801 ggatgcagga tcacccgatc gccggggtcg agctcccgct tcgtgccgac accgcgatcc
     1861 agacgccgga tgaccgccac accaactgct gcgtgcgtta ctccagcaac gatcccgatg
     1921 ggaccccgaa cgacatgatg ctggtctcga tgaatcaggc agtgctctcg atggacgccg
     1981 cggagacggc cgccggcgcc gggctgatcg gcgtctggct gaaccaggcc tattcccgcg
     2041 gcaccgtgcg gctgacctcg cgcgatcccc gcgtgcaccc cgaggtgcgc gaatcgatgc
     2101 tctccgacga gcgcgatcga cgacgcatgc gcgaggggat ccggatgctc gcgaagatgg
     2161 cggcgctacc ggaagtgacg agcatctgcg ccttcgatcc ggcgaaggcg aacggtccgc
     2221 tgctcgcggc gctggatggc aacgatgacg atctcgacgc cttcctgctc gcaaacgtca
     2281 gcgacaccca acatggcacg agcacctgcc ggatgggagc agccggcgat cccggagccg
     2341 tcgtcgatac cgagtgccgc gtgctgggca tcgacggact ccgcgtgatc gatgcttcga
     2401 tcttcccctt cgtcttgcgc tcgaacacga atctcgcgac gatcatgctt ggcgagtaca
     2461 tggccgaccg cctgagtcag taacagccac gaaggaagcc acacccgatg cccagagtcg
     2521 ccgtcgccca gaccggatcc gtgcttttcg atacacccgc cacgctcgac cgcatgcagg
     2581 cgatctgcga ggaggcgcag cgcgaacgcg tcgatctcct cgtcttcccg gaggccttca
     2641 tcggcggcta tccgaaggga acggacttcg gcgcccgaat cggctcgcga acgccggaag
     2701 ggcgtgacga cttcctccgc tattaccagt cggcgatcgc gattcccggt ccggagaccg
     2761 cgcgcatcgg cgagttcgcg cgggagatga gttcctatct cgtcgtcggc gtgatcgaac
     2821 gtgatagcgg cacgctctac tgcacggcgc tctatgtcgg acccgacggc gcgatcctcg
     2881 gcaagcatcg caagctgatg ccgaccgctg ccgagcggct gatctggggc ttcggcgacg
     2941 gctcgacgat cgacgtcgtc gattccccac acggtcgctt cgcggtgacg atctgctggg
     3001 agaactacat gccggcgctg cggacggcga tgtacgcgca gggcatccag ctctggtgcg
     3061 cgccgaccgt cgatgaccgc gagatctggc aggcgagcat gcgccacatc gcctacgaag
     3121 gacgctgctt cgtgctcagc gcctgccagt acatgacccg cgccgacgcc cccgacgact
     3181 acatctgcat ccagggcgac gatccggcga cggtcctgat tcgcggcggg agcgtcatcg
     3241 tctccccgct cggtgaggtc ctggccggtc cgatctccaa cgagtccggc ctgctcgtgg
     3301 ccgagatcga tctcgacgac tgcatccgtg gcaagtacga cctcgacacg gtcggccact
     3361 acgcgcgacc ggatatcttc cagctcagcg tcgacacgcg cccgaaacca ccagttctgt
     3421 aggcgtcttc ccaaacacgc cttcccgggc ttgtcgaggg accagccggc ggccacatgc
     3481 tcccggcaca gcccggtgga cgtggtcggg cggagtccgg ctgcgctggg tgcgcttcgc
     3541 ccggggatcc ttcggcaagc tcaggatgag gaccaggaag tggagcggca ggcacatctt
     3601 ccgacccttg ctctcgtggt ccgtcaccgg tgtcttgccg gctggcatcg tcgagcccac
     3661 gatcagatgc agcttcgccg tatgggccac acccttcggc gaacgcaggg caggctcgtt
     3721 ggtggcccac tccgggatca ccgtggcact tcagctgtga cgcaacccta gtggtcggaa
     3781 tggtccacaa attccggcag gatctcgccc gcctggatcg gcacgcccag gaagttctcc
     3841 accggcggaa tcgaacacga ccatccgctg ctgtaggcgc acaacgggtt gtacgagaga
     3901 ttgaaatcca ccaccagctt gccgtcaggt gtcgccgcca cctcgaggta gcgaccgatc
     3961 tcataggtct ccttgcccga cgtggt
//
LOCUS       DASTMD010000051         4826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1611760, whole genome shotgun sequence.
ACCESSION   DASTMD010000051 DASTMD010000000
VERSION     DASTMD010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611760"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFQ54_00780"
     CDS             <1..544
                     /locus_tag="VFQ54_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEU0163541.1"
                     /translation="RVSGMQVALPLPVVPEDTVKLADAPAWERMAADYAVLGMSPRHH
                     PIDLLRSRLPDDLVTTRGLQSLRNGMPVRIGGLVVCRQRPGTAKGVTFLLIEDELGVA
                     NVVVYSKLYEDQRTLVRAEPFILVEGHVQWEDRNLNIIADRFRPLRSVLADEKAGDAD
                     ESASVVKLITDLSPSSHNYR"
     gene            complement(964..2154)
                     /locus_tag="VFQ54_00785"
     CDS             complement(964..2154)
                     /locus_tag="VFQ54_00785"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEU0163542.1"
                     /translation="MIDPNQSDSPGDSIEETADQMPRVLNPSATMLTTRELNRATLAR
                     QALIERSDLTPFAMVEHLVGMQAQAPNPPYIGLWTRLGNFNPDDLSGLILDRSIVRIA
                     LMRSTVHMVTAEDALLLSPLTRSIAEAHLSQKSFHTEAIAGVDLARLKEIARDLLEQR
                     PRTLNELRPLLGEHFPDSDTEQLAWAVRLLLPLVQVPPRGIWGTGGLPICTTLGSWLG
                     RDLHPEPAIDGIVLRYLRAFGPASVRDAQVWMGLTRLGDVFARLRPQLVSFVNDAGVE
                     LFDLPDASRPDAETPVRPRILPAWDNVLISYADRRRMISEEFRPRVFSQNGIIQSTLL
                     VDGFVAGVVTMKRLKKTASVEIELLTTISPDDRELAAIEAHALLAFAEPNAETHEVRF
                     TSAI"
     gene            complement(2182..2703)
                     /locus_tag="VFQ54_00790"
     CDS             complement(2182..2703)
                     /locus_tag="VFQ54_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0163543.1"
                     /translation="MTNQFAPGIESFVVPDDGVFPNSTLPVVLYRQAIALARNDPAAS
                     IEERFHDEGWEGVWRNGIFTFHHYHSTAHEVLGIARGTVRVQLGGPTGPELELTPGDI
                     VVLPAGTSHFNAGQSGDLIVVGGYPSGQRPDLLRGEPGDRPAADDAIAAVPRPQSDPV
                     LGDRGPLIELWAK"
     gene            complement(2819..3412)
                     /locus_tag="VFQ54_00795"
     CDS             complement(2819..3412)
                     /locus_tag="VFQ54_00795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU0163544.1"
                     /translation="MSDNITPADNGGARLRIAIIVGSNRSGRFGETISNWFVAQAKQI
                     EGIDFDVIDLASLDIPMDFSGGGDSADFRSRIGAADGFIVITPEYNHSFPGYLKHAID
                     SVKDEWKTKPVGFVSYGGVSGGLRAVEGLRLVFAELHATTIRDAVSFHQAHGLFDENG
                     QLTNPDGPNATAAYVINELAWWAEGLKTARATVKYPA"
     gene            3543..4004
                     /gene="soxR"
                     /locus_tag="VFQ54_00800"
     CDS             3543..4004
                     /gene="soxR"
                     /locus_tag="VFQ54_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HEU0163545.1"
                     /translation="MAKPTWDTVELTPGELSDRSGVAVSALHFYERQGLIRSRRTEGN
                     QRRYPRETLRRVAFIRSSQHVGIPLARIREALETLPEGRTPTVEDWAHLSQGWRHDLD
                     RRIEQLVRLRDRLTTCIGCGCLSLDRCALSNPQDRVARLGPGARFLEVEID"
     gene            complement(4194..>4826)
                     /locus_tag="VFQ54_00805"
     CDS             complement(4194..>4826)
                     /locus_tag="VFQ54_00805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0163546.1"
                     /translation="ASTIGADDVVIASGIWSPRLAATVGFDLPLVPVEHPYVYGPPPE
                     KRISRATTPFIRWPEEHVYARYHGPRFGIGTYDHVPRPVAPEDLATGAEFPWPGAIFD
                     EAIDRARSLLPASNRFPSDLKLNGVFAITPDNAPLIGRIPAADGLWTASAIWVTHAAV
                     SAKVLAALMTDRESPVAGISALDPARFAGRDPDELQRRALGHYRDIYTVS"
BASE COUNT          835 a         1455 c         1646 g          890 t
ORIGIN      
        1 tcgggtctcg ggcatgcagg tggcgttgcc gcttcctgtc gtcccggaag atacggtcaa
       61 gctggccgat gccccggcgt gggaacggat ggcggcggat tacgcggtgc tcgggatgtc
      121 gccccggcat cacccgatcg atctgctgcg ttcccggttg ccggacgacc tcgtcaccac
      181 gcgtggcctg caatcgctcc ggaacggcat gccggttcga atcggcggcc tcgtggtctg
      241 tcgacagcgt cccggtacgg cgaagggggt gacgttcctg ttgatcgagg acgagctggg
      301 ggtggcgaat gtcgttgtct acagcaagct ctacgaggat cagcgaacgc ttgtccgtgc
      361 ggagccattc atcctggtcg aaggccacgt gcagtgggag gatcgcaatc tcaatatcat
      421 cgccgatcgt ttccggccac tacggtcggt gctggcggat gagaaggccg gcgatgccga
      481 cgaatcggcg agtgtcgtca agctgatcac ggatctctcg ccgtcgtccc acaactaccg
      541 gtaggatggg tgtcttttgg gccgtcgcgg gtcagctggt ctgactcatc gagcggagct
      601 cgatatgtgc cggcacaacc atattctgcc gtcagaaccc tccctggaac cgtagacgcg
      661 atgcttgtca tcgctccagg ttggctctgc caacccgact gatcgtcggc gaacgtcccg
      721 gcgcggcaat ctgctcggac agtcctgtta gcggctggag atagactcca gccctacaaa
      781 ccccgtttgc cggggacgcg ctccaggcac gatttcatcc gcatctacag cattgcattg
      841 aatcacggca gcgcgtgcgg attgggacgc gtctatggtt cctcgtcgat tacgaagaca
      901 cgagacaggt gatcgaacga ttgtgaagct gtttaccctg tccgtccgtc tcaccagcca
      961 ggatcagatg gcgctggtga agcgaacctc atgtgtctcc gcgttcggtt ccgcgaaagc
     1021 gaggagggca tgtgcctcga ttgccgcgag ctcgcgatcg tctggcgaga tcgtggtgag
     1081 gagttcgatc tcgaccgacg cggtcttctt cagccgcttc attgtgacca cccccgcgac
     1141 gaaaccgtcg acgagaaggg tgctctggat gattccgttc tgggagaaga cgcgcggccg
     1201 aaattcctcc gagatcatcc ggcgtcgatc ggcgtaggag atcagcacgt tgtcccacgc
     1261 cggcaggatc cgtggtcgca cgggcgtttc ggcgtccggt cgtgatgcat cggggagatc
     1321 gaagagttcg acgccggcat cgttgacgaa ggagacgagc tgcggacgca gccgcgcgaa
     1381 gacgtccccg aggcgggtca gccccatcca gacctgcgcg tcgcggacac tcgccggtcc
     1441 gaacgcccgc aggtagcgga gcacgatccc atcgatcgcc ggttccggat ggaggtcgcg
     1501 gccgagccac gatccgaggg tcgtgcagat cggcagacca cccgttcccc agatcccgcg
     1561 cggaggaacc tggacgaggg gaagcagcag gcgaaccgcc caggcaagtt gctcggtatc
     1621 gctgtccggg aagtgctcgc cgaggagcgg acgtagctcg ttgagcgtgc gtggccgttg
     1681 ctccagaaga tcgcgcgcga tctctttcag tcgtgcgagg tccaccccgg cgatcgcttc
     1741 ggtgtggaag ctcttctggc tgagatgggc ctcggcgatc gatctcgtca gcggactgag
     1801 caggagggcg tcctcggccg tcaccatgtg gacggtgctg cgcatcaggg caatccgaac
     1861 gatcgagcgg tcgagaatga gaccggagag atcgtctgga ttgaagttcc cgaggcgcgt
     1921 ccagagtccg atatagggtg gattcggcgc ctgcgcctgc atcccgacga ggtgttccac
     1981 catcgcgaac ggtgtcaggt cactgcgctc gatcagggcc tgccgggcga gcgtggcgcg
     2041 attgagttcg cgggtcgtga gcatcgtcgc actggggttg agcactcggg gcatctggtc
     2101 tgccgtctcc tcgatcgaat caccgggcga atccgactgg ttcggatcga tcatatgatc
     2161 ctgtgaggac agggattgtc cttatttcgc ccagagttcg atcagcgggc cgcgatcgcc
     2221 gaggacggga tcgctttgcg gacgtggcac cgcggcgatc gcgtcgtcag ccgcggggcg
     2281 atcgccgggt tcgccacgga ggagatcggg gcgttgtccg gaaggatagc cgccgaccac
     2341 gatgaggtcg ccgctctgtc cggcgttgaa atgggatgtc cccgccggga gcacgacgat
     2401 gtcacccgga gtcagctcga gttccggacc ggtcggtccg ccgagctgga cgcggacagt
     2461 tccgcgggcg atcccgagga cttcatgggc ggtgctgtgg tagtgatgga aggtgaagat
     2521 gccgttccgc cagacgccct cccagccctc gtcgtggaag cgctcctcga tcgaggcggc
     2581 aggatcgttg cgggcgagtg cgatcgcctg gcgatagagg acgaccggaa gcgtgctgtt
     2641 ggggaagacc ccgtcgtcgg gcactacgaa cgattcgata ccgggtgcga actggttcgt
     2701 catggtgatg gctcctcctg cgatcggtgc gttcgaccat catgccatga cgtatggacg
     2761 cgaaacgggg cggacacccg gcccgccccg ttttcttcgc gttgatttgg agaagtcgct
     2821 acgccgggta cttgacggtg gcgcgggcgg tcttgaggcc ctccgcccac cacgcgagct
     2881 cgttgatgac gtaggccgcg gtggcgttcg ggccatccgg attcgtcagc tggccgttct
     2941 cgtcgaagag accgtgcgcc tggtggaagc tgacggcgtc gcggatcgtc gtcgcgtgaa
     3001 gctcggcgaa gacgaggcga agtccctcga cggcacggag acctccggag acgccgccat
     3061 aggagacgaa gccgaccggc ttggtcttcc attcgtcctt gacgctgtcg atcgcgtgct
     3121 tcaggtaacc cggaaagctg tgattgtatt ccggggtgat gacgatgaaa ccgtccgcgg
     3181 cgccgatgcg cgagcggaag tccgccgaat cgccgcctcc cgagaagtcc atcgggatgt
     3241 cgagcgacgc cagatcgatg acgtcgaaat cgatgccctc gatctgcttc gcctgcgcga
     3301 cgaaccagtt ggagatggtt tcgccgaagc gaccggagcg gttgctgccg acgatgatgg
     3361 cgatccgcag acgggcgccg ccgttatctg cgggagtgat attgtctgac acggaaactc
     3421 cttcagagag agtaagtaac ctacggccgg taagtatgtg gcgaggatcg acaggtgtcc
     3481 acccccaacc gatactggta ttctgagacc tgaacaatta ttgaggtcaa gggggtatgg
     3541 atatggcgaa gccaacctgg gacacggtcg aattgacccc cggcgaactc tcggaccgaa
     3601 gtggtgtcgc ggtctcggcg ttgcacttct acgagcgtca gggattgatc cgcagccgga
     3661 gaacggaggg gaaccagcgt cgctatccgc gtgagacgtt gcgccgagtc gccttcatcc
     3721 gctcttcgca gcacgtcggg attccgctcg cgcggattcg cgaggcgctg gagacgcttc
     3781 cggaaggccg cacgccgacc gttgaggact gggcgcacct ctcacaaggg tggcggcacg
     3841 atctcgatcg ccgcatcgag caactggtcc gtttgcgcga ccggctgacg acctgcatcg
     3901 ggtgcgggtg cctctcgctc gatcgctgtg ccctctcgaa tccgcaggat cgcgttgccc
     3961 gcctcgggcc gggtgcgcgc ttcctcgagg tcgaaatcga ttgaggtcgg acgatgccca
     4021 gcctcccgag aggtgggaca tagaagtccc accgtactca cgaccgattc tcccgtcgtg
     4081 gcggggcacc tcgtgcgacc tatcgggctg catccgatag gtcacacggg cggtgggtac
     4141 atcgcgagct tcttcaccga atcatgatga tcgggtcagc tggatgcgcc gcatcacgag
     4201 accgtgtaga tgtcccgata gtgaccgagc gcgcggcgct ggagctcgtc ggggtcccgg
     4261 ccggcgaacc gcgccgggtc gagtgcgctg atcccggcaa cgggtgactc ccggtccgtc
     4321 atcaacgcgg cgaggacctt cgcgctgacc gcggcgtgcg tcacccagat cgcgctcgcg
     4381 gtccagaggc cgtcggcggc tgggatccgc ccgatcaggg gcgcgttgtc cggggtgatc
     4441 gcgaagacgc cgttcagctt gagatccgat ggaaagcggt tcgaggccgg gagaagcgat
     4501 cgtgcccggt cgatcgcctc gtcgaagatc gcgccgggcc acgggaactc ggccccggtc
     4561 gcgagatcct cgggcgcgac cggtcgcggg acgtgatcgt aggtgccgat tccaaaccgt
     4621 gggccgtgat aacgagcgta gacgtgttcc tccggccagc ggatgaacgg tgtcgtcgcg
     4681 cgggagattc gcttctccgg cggcggccca tagacatacg gatgttcgac ggggaccagc
     4741 ggcaaatcga agccgaccgt cgccgccagg cggggactcc agatcccgct cgcgatcacg
     4801 acatcgtccg ctccaatcgt cgacgc
//
LOCUS       DASTMD010000052         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2236348, whole genome shotgun sequence.
ACCESSION   DASTMD010000052 DASTMD010000000
VERSION     DASTMD010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2236348"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2382
                     /locus_tag="VFQ54_00810"
     CDS             <1..2382
                     /locus_tag="VFQ54_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163547.1"
                     /translation="GVDIISAMQTDVPGITVGLASLLTDAQIKYLAVAHNYAGISVPF
                     LNDGQDLKRPFWWQAPDGEKLLVWYTDTLIGDAYMEAMHVGFGSGYDAALGSLPEYLN
                     SVIEHGYPYGRNESWMNLLLDGIEITKKPYEYDILHLRVHGAYADNASSSILPATIIR
                     EWNEHWAWPKLRSSIDRDFFTEVETKVGDELDVYQGDWSDWWAIGVGSAAFALGRNRQ
                     SQSDIKTAQTLHALSDVLTDEPNPSVPDDVKGAYEEMALFDEHTWGAADPWGLAASKT
                     ASGAYQWTRKEAFAYTAEERTQMLLNSGLQRLAPLAARETTAPREETLLVFNPSSWPR
                     IDLAKIFIPRHEPKNIDFDLIDLTTGATVPSIYEEPENPNHRPIGRWIRFVAKDVPAI
                     GYRRYALVPAARGQQPEDSGDPTTIANEHLRVAIDPKTASIASIRDLGGNRELVASDA
                     PFGFNRYIYDRYTSAPGFNHLSSRIGGSAGRWLLGSRKAGDYGLVTSHEANGVWDRLT
                     LRYTGDGADWAETTLTLPHGASRLHIQNHLYKPVTMQKESVYYAFPFNVDDAVINQEI
                     TGGVVNDNSPHVPGSLDHYRTLAHWATITSENSKPIAWATNEAPLIQLGNIHIPYAPF
                     PKTIPDWDAHPATIYSWALNNIWDTNFPPEQGGELTFSYAIGVGDDDHLRLGRDTGAS
                     VATPLVGFCAPVGAVATDDMPESGSFLTLDHPAVQITHLAPARDGSGIAVFLYSLADR
                     TMTAEIAITRLPVTAARTGSFLETDFEEVPLANGRIEVTIGPGEFKSVVLEVR"
     gene            complement(2408..3205)
                     /locus_tag="VFQ54_00815"
     CDS             complement(2408..3205)
                     /locus_tag="VFQ54_00815"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEU0163548.1"
                     /translation="MSKRSPSFAMRPRPDLSQQLSHQIVELIAASELAPGDRLPTMKD
                     LAQRFNVATPTMREALRLLQATGMIDIRHGSGIYLLRREQGMMIANPHRGDLDAKAIL
                     DLLEARLLIEPYLAGLAATRVAPGDVSRIAAILDDAARLLEGQDLPLGAANMRFHTAI
                     ATIAGNQILALMLDSLVELYGSEQIAILELFNARRRDHQEHIGIFEAIRAGDAGEASR
                     RMAEHLIQVRDVIAERLRDQVDAVSQRDSRAGSPAVNDSGDRPYGLS"
BASE COUNT          694 a         1260 c         1164 g          611 t
ORIGIN      
        1 ggtgtcgaca tcatctctgc gatgcagacc gatgttcccg gcatcaccgt cggcttggcg
       61 agcctcctca ccgacgccca gatcaagtac ctcgccgtcg cccacaacta cgccgggatc
      121 tccgtgccgt tcctcaacga cggccaggat ctgaagcgac cgttctggtg gcaggctccg
      181 gacggcgaga aactcctcgt ctggtatacc gacaccctga tcggcgatgc ctacatggag
      241 gcgatgcatg tcggcttcgg cagcggctac gacgccgcac tggggtcgct tcccgaatac
      301 ctgaactcgg tcatcgagca cggctatccc tacggcagga acgagagctg gatgaatctc
      361 ttgctcgacg gcatcgagat cacgaagaag ccatacgagt acgacatcct gcatctccgc
      421 gtccacggcg cctatgccga caacgcctcg tcgtcgatcc tcccggcgac gatcatcagg
      481 gaatggaacg agcactgggc ctggccgaag ctgcgctcct cgatcgatcg cgacttcttc
      541 accgaggtcg agacgaaggt cggcgacgag ctggatgtgt accagggcga ctggtcggac
      601 tggtgggcga tcggcgtcgg ctcagcggcg tttgcgcttg ggcgcaatcg ccagagccag
      661 tccgacatca agacggcaca gacgcttcac gcgctctccg atgtcctcac cgacgagccg
      721 aatccgtctg ttccggacga cgtcaagggc gcctacgagg agatggcgct cttcgacgag
      781 cacacatggg gcgcggccga cccgtgggga ctggccgcgt cgaagaccgc atccggcgcc
      841 taccagtgga cgcgaaagga ggccttcgca tacaccgccg aggagcggac gcagatgctc
      901 ctcaacagcg gcctgcagcg actcgcaccg ctcgccgcgc gcgagacgac cgcgccgcgt
      961 gaggagacgc tcctcgtctt caatccgagt tcctggccgc ggatagatct ggcgaagatc
     1021 ttcattccac gccacgagcc gaagaacatc gacttcgacc tgatcgacct gaccaccggc
     1081 gccaccgtcc catcgatcta cgaagagccc gagaatccga atcaccggcc gatcgggcgc
     1141 tggatccgct tcgttgcgaa ggatgttccc gccatcggct accgtcgcta cgcgcttgtc
     1201 ccggcggcgc gcgggcagca gccggaagat tccggcgacc cgacgacgat cgccaacgag
     1261 catcttcgtg tggcgatcga tcccaagacg gcgtcgatcg cgagcatccg tgacctcgga
     1321 ggcaatcggg agctcgtggc atcggacgcg ccgttcggct tcaatcgcta catctacgac
     1381 cgctacacga gtgcgcctgg cttcaaccac ctctcgagcc ggatcggtgg cagtgccgga
     1441 aggtggcttc ttggatcgcg gaaggcaggc gactacggac tggtcacctc ccacgaagcg
     1501 aacggggtct gggatcgcct gacgcttcgc tacacgggcg acggcgccga ctgggctgag
     1561 acgacgctca cccttcccca cggggcctca cggctccaca tccagaacca cctctacaag
     1621 ccggtgacga tgcagaagga gagcgtctac tacgccttcc ctttcaacgt cgacgatgcg
     1681 gtcatcaatc aggagatcac cggcggtgtg gtgaacgaca acagcccgca tgtcccggga
     1741 tcgctcgatc actaccgcac gctcgcacat tgggccacga tcacctcgga aaactcgaag
     1801 ccgatcgcct gggccacgaa tgaagcgccg ctgatccagc tcggcaacat ccacatcccg
     1861 tacgcgccgt tcccgaagac gattccggac tgggatgcgc accccgccac catatactcg
     1921 tgggccctga acaacatctg ggacaccaat ttcccaccgg aacagggcgg cgagttgacc
     1981 ttctcctatg cgatcggcgt cggcgacgac gatcatctcc ggctcggacg cgacaccggc
     2041 gccagcgtcg cgacgccgct cgtcggattc tgcgcgccgg tcggcgctgt cgcgaccgac
     2101 gatatgccgg agtccggcag cttcctgacg ctcgatcatc cggcggttca gatcacgcat
     2161 ctcgcgccgg cgcgcgatgg gtccggaatc gcagtctttc tctactcgct tgccgaccgg
     2221 accatgacag cagaaatcgc gatcacccgg ctgccggtga ccgctgcccg gacggggagc
     2281 ttcctcgaaa ccgacttcga ggaggtcccc ctcgcgaacg gccggatcga ggtcacaatc
     2341 ggccccggag agttcaagtc ggtggtgctg gaagtccgct agcggcgcag cgaagaccgg
     2401 tcgactccta cgacaatccg tagggtcgat ctccggaatc gttgactgcg gggctcccgg
     2461 cacgggagtc gcgctgcgag acggcatcaa cctggtcgcg aaggcgctcg gcgatcacgt
     2521 cgcgaacctg gatgaggtgt tccgccatcc ggcgcgacgc ctcacccgcg tctcccgcgc
     2581 ggatcgcttc gaagataccg atgtgctcct ggtgatcgcg acgacgcgcg ttgaagagtt
     2641 cgagaatcgc gatctgctcc gagccataga gctccacgag cgagtccagc atcagcgcca
     2701 gaatctggtt cccggcgatc gtcgcgatcg ccgtgtgaaa gcgcatgttg gcagcgccga
     2761 gcgggagatc ctgcccttcg aggagccggg ccgcatcgtc gaggatcgcg gcgatccggc
     2821 tcacgtcacc gggagccacg cgagttgcgg cgagacccgc gaggtagggc tcgatcagca
     2881 ggcgcgcctc gagcagatcg agaatcgcct tcgcatcgag atcgccgcgg tgcggattcg
     2941 cgatcatcat cccctgctcc cggcgcagga gatagatgcc ggatccgtgc cggatgtcga
     3001 tcatgccggt cgcctggagc aaccgcaacg cctctcgcat cgtcggcgtc gcgacgttga
     3061 accgctgggc aagatccttc atcgtcggca agcgatcgcc cggcgccagc tccgacgcgg
     3121 cgatgagttc gacgatctga tgcgagagct gctgggagag gtcaggtcgg ggccgcattg
     3181 cgaacgaagg cgatctcttg ctcatgtcca ccgtgtcgcc cccgcccact gccgtcaccc
     3241 gcccgaagtc gggaacagta tgaccgcttc gcgccgccgg ggcgcgaccg ctcaatcgga
     3301 aagagtgggg ccaggtgcgg cggtcatgcc cttgagccag gtcggcaatg gtacccacat
     3361 gcgccaatag tggccgcagt ttccgtcatc cggagcctgt ctgtagagac cgggatcagt
     3421 tgacccccgc gtgttcccgg ctcaacgcct cgcaggacac gtcctcccga gcctgtcgag
     3481 ggatcagccg gcatctacgt gcttcctgcg cgacgcctcg caggacacgt cctcccgagc
     3541 ctgtcgagga accggttggc ctccgcgtgt ttccgggaag acccgccgcc aaaggggcag
     3601 gcaggcgagg cgtcgcgaat gggacacggt cccgggcgaa caccaggttc gctcctacgg
     3661 acgcgcgcct aaagctcgtt tcaacgatcg gggcgatcgc cagagcctgt cccgagcgaa
     3721 gccgaggga
//
LOCUS       DASTMD010000053         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2415178, whole genome shotgun sequence.
ACCESSION   DASTMD010000053 DASTMD010000000
VERSION     DASTMD010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2415178"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VFQ54_00820"
     CDS             <1..999
                     /locus_tag="VFQ54_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0163549.1"
                     /translation="SQGIDLGDYTTRQSAADLDFVRQALGAKRVDLLGTSYGTWLALE
                     MMRDYGSKVRSAVLNSPVPPQTDLLGGQIVAFQDSLNEVYKGCAADAQCNAAYPHLET
                     TLTALVKKLNAKPLSVTYKDPTTGKDATGDVDGTTIMSLIYQLVFIGPFVPYVAPLIG
                     TVASGDDSFLSQLVPLLVSSNEGISNGLYYAVMCQDEVPFTSVTAIKAQAKKAKVSQD
                     VIDEGLSSSEGTIDVCKDWDLKASPDVENTAVTSSIPTLIVTGKFDPITPTSYGKLLE
                     TTLSNATLITSPVAGHDPASTTGTCGVGIIHRFLNEPADKLDTSCLKDLSLDFSPQ"
     gene            1380..>2780
                     /locus_tag="VFQ54_00825"
     CDS             1380..>2780
                     /locus_tag="VFQ54_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0163550.1"
                     /translation="MLPDHREFAFSNMQPPEIASQFIDRPRLLAAFRTASHRRIMHIC
                     APAGYGKTILTAQWCAQLTHPVAWLTLTSDHNVRDRFLADFAQALDVAGTTPGASSSL
                     TGEVTMTAVETRLRDVSLPRTVVIDNVAAVGAEDVRFALKMLVEGAPRGVSFVLTSRE
                     TLPWRIASEIAAHDLLIVQREDLAFTRSEQLAAIDRLHPSEANRAALTSLIETSDGWP
                     VIVGLAEYLLTMRDEDTTRWNRDDGGPDADFSRQALDELLDRYVDEEVLSALGDTRRR
                     ETLRLSLLARVNFDLCNLLFGRSESAELLKTLINGMLLTLDDRAGADWSLSPKPLRAS
                     LQRHAHRELLRSERDAVRATAALWHIRENRLDEAIGDLAAIDETLVPDDISSWLLAET
                     PDEQRHPQLIRILEGTHGSEVGDLDRVILFVAWIFLLRGRIDRCRMLINRFEPGWRRR
                     GLSSLVAYASILRAGCY"
BASE COUNT          513 a          891 c          863 g          513 t
ORIGIN      
        1 agccagggca tcgacctcgg cgactacacg acccgccaga gcgcagccga tctcgacttc
       61 gtgcgccagg cgctgggagc gaagcgggtc gacctgctcg ggacctccta tggcacctgg
      121 ctcgcgctcg agatgatgcg cgactacggc tccaaggtcc ggagcgcagt gctcaactcc
      181 ccggtgccgc cgcagaccga cctcctcggt ggccagatcg tcgcgttcca ggattctctc
      241 aacgaggtct acaaggggtg cgcggccgac gcacagtgca acgcggcgta tccgcacctg
      301 gagacaacgc tcaccgcgct cgtcaagaag ctgaacgcca agccgctctc ggtcacctac
      361 aaggatccca ccaccggcaa ggacgccacg ggcgatgtcg acgggacgac gataatgagc
      421 ctcatctatc aactcgtgtt catcggtccc ttcgtaccct acgttgcccc gctgatcggc
      481 accgtggctt cgggcgacga ttccttcctg tcgcaactgg ttccgttgct cgtctcctcg
      541 aatgaaggaa tctcgaacgg tctctactac gcggtgatgt gccaggacga ggtcccgttc
      601 acgtcggtga ccgcgatcaa ggcgcaggcg aagaaggcca aggtgagtca ggatgtcatc
      661 gatgagggcc tgagcagcag cgagggcacg atcgatgtct gcaaggattg ggatctgaag
      721 gcatcgccgg atgtcgagaa tacggcggtc accagcagca ttccgacgtt gatcgtgacc
      781 ggcaagttcg atccgatcac gccgacctcc tatgggaagc tgctggagac cacactctcc
      841 aacgcgaccc tgatcacgag tccggttgcc gggcatgatc ccgcatcgac gacagggacg
      901 tgtggcgtgg ggatcatcca ccgcttcctg aacgaaccgg cggacaagct cgacacctcc
      961 tgcctcaagg atctctcgct cgacttctcg ccgcagtagc aggatagcca tcgcggcaga
     1021 acgacgccgg accgccattc cccggggaat gacggtccgg cgtttcgttt gtgacgtccg
     1081 gatgcgatcg aaggcgatat ggacataccg gccagttgag tccaaaaccg accaggccat
     1141 gaagagatat cgcgattggc cggaagcaag cgaattaatc cggacacatt ttatcaggtt
     1201 tctgatagga tatatcgtga cagggacact tattgcccga atgggcattc tcgagacacg
     1261 ccaggatttc agtacccgtc cgggcatgca atcggggtgg ttttccgccg ggtcgccggg
     1321 acgtgtgaat gaaggccctt ctccaccagc accagtctaa cggacgtgac gacaggtcgg
     1381 tgttaccaga ccaccgcgaa ttcgcgttct ccaacatgca gcctcccgag atcgcctcgc
     1441 aattcatcga tcgaccgcgc cttctggccg cgtttcgcac ggcgagtcat cgccgcatca
     1501 tgcacatctg tgccccggcc ggatatggca agacgatcct gactgcccag tggtgcgccc
     1561 agctcaccca ccccgtcgcc tggctcaccc tcacgtcgga tcacaacgtt cgcgatcgct
     1621 ttctggccga cttcgcacag gcgctggatg tcgccgggac gacccccggt gcgtcatctt
     1681 ccctgacggg agaggtgacg atgaccgcgg tcgagacgcg cctgcgcgac gtcagcctcc
     1741 cccggacggt ggtcatcgat aacgtcgccg cggtcggcgc cgaggatgta cggttcgcgt
     1801 tgaagatgct tgtggaagga gcgccacgag gggtgagttt cgtcctgacg tcgcgggaga
     1861 cgttgccatg gcggatcgca agtgagatcg ccgcccacga tctcctgatc gtccagcgcg
     1921 aggatctcgc gttcacacgg tccgagcaac tggcggcgat cgatcggctc cacccgagcg
     1981 aggcgaatcg cgccgcgttg acgtccctga tcgagacctc cgacggctgg ccggtgatcg
     2041 tcggtctggc ggagtacctg ctgacgatgc gggacgagga caccacgcgg tggaatcggg
     2101 atgacggcgg tcctgatgcc gacttctcgc ggcaggcgct cgacgaactg ctggatcgct
     2161 acgtcgatga ggaagtgctc tcggcgttgg gcgacacccg tcggcgcgaa acgctcaggc
     2221 tctccctgct ggcgcgcgtc aacttcgatc tctgcaatct tctctttggg aggagcgaga
     2281 gcgccgaact cctgaagacg ctcatcaacg gcatgctcct cacgctcgac gatcgcgcgg
     2341 gggccgactg gagcctctcg ccgaaaccgt tgcgcgcgag cctccagcgc cacgcgcatc
     2401 gggaactcct ccggtcggaa cgggacgccg ttcgcgccac ggcggcgctc tggcacatcc
     2461 gggagaatcg cctggatgag gcgatcgggg atcttgcggc catcgacgag acgctcgttc
     2521 cggacgacat cagttcctgg ctgctggcgg aaaccccgga cgagcaacgc catccccagc
     2581 tgatccggat tctcgagggc acgcatgggt ccgaggtggg agatctcgac cgggtcatcc
     2641 tctttgtcgc ctggatcttc ctgttgcgtg gacgtatcga tcgctgccgg atgctcatca
     2701 atcgcttcga gccggggtgg cgccgtcgcg gtctgtcgtc gcttgtcgcg tatgcctcga
     2761 ttctacgggc tggttgctac
//
LOCUS       DASTMD010000054         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_808655, whole genome shotgun sequence.
ACCESSION   DASTMD010000054 DASTMD010000000
VERSION     DASTMD010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808655"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1262)
                     /locus_tag="VFQ54_00830"
     CDS             complement(123..1262)
                     /locus_tag="VFQ54_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163551.1"
                     /translation="MTQRPFSSQPDDPDDDAFDDALSALQRGDRDAARTIDAEMASTI
                     DQLFGWAELSGFASEPATHASFGETSANPSRPSSESRRRPGPPTRPTVSRESSIYPTS
                     NSRTRRSYSIMGFFSGLAIAALIAASVFGARPLFDHHTPTPTELAFQELPGSPTVQIA
                     GGEDVVPNQATPSKTPMPNFTPDPNQTYVVPPLDASDCTAKPRTRQEMLDILSNPPDV
                     LSPDPNPEDGIFSGNEVADQSTIDEFNQLLRAWQTCAAFGLTGQTWAYESKTYIREDF
                     YGDPRISKAYSPSTLSELIDARYQIDEDKITRGNLGDNDILTIDPNNPVMITDDTVQI
                     RSAQAMVIFVSPQTGSLEGGPYHIAFVWQDGGWKVHLQDPGYSIG"
     gene            complement(1259..1858)
                     /locus_tag="VFQ54_00835"
     CDS             complement(1259..1858)
                     /locus_tag="VFQ54_00835"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0163552.1"
                     /translation="MAQTDGSHLVSESIPANPDEALVQRARAGDSHAFDELYQRYQRE
                     IRAFLINRVRGDLAMAEDLTSEVFTKLFRFLDQYRAGSSFRGWLYQIARNTAIDHARR
                     ARITTSLDDAGTIASPVEALDEQAIAAESREALLRALAVLPPGPRRIVELRLKGFGPN
                     EIAAELGMELSAVKSAQHRAFRKLRAELHDFMSDREMTS"
     gene            2034..2570
                     /locus_tag="VFQ54_00840"
     CDS             2034..2570
                     /locus_tag="VFQ54_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0163553.1"
                     /translation="MGTLAFGLLHERDIDRIRDIDRSEIIDALYAVDDGKLVLREHSH
                     TSPPWTPEMVERYVERARAVVASGGVAFGAWDGPRLVGIAVLDVRPVGDDPSILNLDF
                     LHVSDGYRGRGISSDLMRLIRATAIQRGARALYISATPTRNTVDVYRHMGATVIDAPD
                     PAMFAREPEDIHLILSLR"
BASE COUNT          497 a          835 c          963 g          579 t
ORIGIN      
        1 gaagtccacc cttacggacg aacgctgtat gcaaacccat cgttagcgag aagtggactt
       61 cctcggagcc ggagtttgct cgcggcgtgg atagggtgcg tgtttgacgg accaacggcg
      121 ggttacccga tcgagtaacc cggatcctgc aagtgaacct tccatcctcc atcctgccac
      181 acgaacgcaa tgtggtaggg gccaccttcg agcgatccgg tttgtggcga gacgaaaatc
      241 accatggctt gcgctgacct tatttgcacg gtgtcatcag tgatcatgac cgggttgttg
      301 ggatcgatcg tgaggatgtc gttgtcaccg aggtttccgc gggtgatctt gtcctcgtcg
      361 atctggtatc gcgcatcaat gagctcgctc agcgtggacg gcgaatacgc tttggagatg
      421 cgcgggtcgc cgtaaaaatc ctcacgaatg tatgtctttg attcatatgc ccaggtctgg
      481 ccggtgagtc caaatgccgc gcaagtctgc caggctcgta gcagctggtt gaattcatcg
      541 atcgtcgatt ggtcggcaac ctcgtttccc gagaagatcc catcttcggg attggggtca
      601 ggactcaaca catctggagg attgctgagg atatccaaca tttcctgacg tgtacggggc
      661 ttcgcggtgc agtccgatgc gtcgagtggt gggacaacat aggtttgatt cggatcgggc
      721 gtgaagtttg gcattggtgt tttggagggt gtcgcttgat tgggcacgac atcctcgccg
      781 cctgcgatct gaacggtcgg cgacccgggg agctcctgga aggcgagttc ggtcggcgtt
      841 ggggtgtgat gatcgaagag cgggcgggcg ccgaagacgg aggccgcgat gagcgcggca
      901 atggcgagtc cagagaagaa ccccatgatc gaatagctcc ttcgcgttcg tgagttcgag
      961 gttggataga tagaggattc acgcgagacg gttggtcggg tcggcggccc aggtcgtcgc
     1021 ctggattcgg atgatggacg cgacggattg gcagatgtct cgccgaagga ggcgtgtgtt
     1081 gccggttcgc tcgcgaagcc gctgagttcg gcccagccga agagctggtc gatcgtcgac
     1141 gccatctcgg cgtcgatggt ccgggcggcg tcgcggtcgc cgcgctgcag cgcggagagt
     1201 gcgtcgtcga aggcgtcatc gtcggggtcg tcgggctggg atgaaaacgg tctctgggtc
     1261 atgacgtcat ctcgcggtcg gacatgaaat cgtggagttc ggcgcgaagc ttccggaagg
     1321 cgcggtgctg ggcactcttg acggcgctca gttccattcc gagttccgcg gcgatctcgt
     1381 tcggaccgaa tcccttcagg cgcaactcga cgatccgacg tggtcccggc ggcaggaccg
     1441 cgagcgcccg caggagcgcc tcgcgggact ctgccgcgat cgcctgctcg tccagggctt
     1501 caaccgggct ggcgatcgtc ccggcatcgt cgagcgatgt cgtgatgcgg gcgcggcggg
     1561 cgtgatcgat cgccgtgttg cgcgcgatct ggtagagcca gccgcgaaac gacgaccccg
     1621 cccggtactg gtcgaggaag cggaagagct tggtgaagac ctcactggtc agatcctccg
     1681 ccatcgcgag gtcgccccgc acgcggttga tgagaaaggc gcgaatctcc cgctggtaac
     1741 gctggtacaa ctcgtcgaag gcatgcgaat ccccggcccg cgcgcgttgg accagcgcct
     1801 cgtccgggtt ggccgggatg gactcgctca cgaggtgcga gccgtcggtc tgcgccatct
     1861 cccacgtccc gtgtgcgccc acgaattccc tgccacacct atccctgttc gaaggtcggg
     1921 tcccgatcgt tcgcgaagca gatgagcgct cacccgctat tcttgttgac gagatgactc
     1981 tgaaaagggt ggcatgccgg tcgagcgatt gtatcgggca acggggaggc gcaatgggaa
     2041 ctcttgcctt cggtctcctg catgaacgtg acatcgaccg catccgggac atcgatcgaa
     2101 gcgagatcat cgatgctctc tacgccgtcg atgacggcaa gctcgtgctc cgggaacatt
     2161 cgcacacctc tccgccgtgg acaccggaga tggtggagcg atacgtcgag cgcgcacgcg
     2221 ccgttgtcgc atcgggcggc gttgcgttcg gagcgtggga cggaccacgt ctcgtcggga
     2281 tcgcggtgct cgacgtgcgt cccgtcgggg acgatccctc catcctgaac ctggacttcc
     2341 tccacgtcag cgatggatat cgggggcgtg ggatcagcag cgacctgatg aggttgattc
     2401 gcgcgacggc gattcagcgt ggcgcgaggg cgctctacat ttccgcgacg ccgactcgca
     2461 acacggtgga tgtctatcgc cacatgggag cgacggtgat cgatgctcct gatcccgcga
     2521 tgtttgcgcg cgagcccgag gatatccatc tcatcctgtc actccgctga tcgtcgcggg
     2581 tgccaatccc catcggcgcg caccccgggc ccggaactcc gtctcacacc gtcgtctgct
     2641 ccgcgtttgt cttccgatga cgcgacggga atgcccggac gtgcatccgg accgcagggc
     2701 gattgcgacg ccctctgtcg gaacgcggga atgaacgacc ccacaaggag gaaacacgtc
     2761 gttgatggac aggcatctgt gctggcgcga gttcgctgtc gccggaattg ccggcgcgac
     2821 ccgatttgcc atcgccgcga gcggcgagcc gagtcttgcg cttcgtcggg gttg
//
LOCUS       DASTMD010000055         5676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1165009, whole genome shotgun sequence.
ACCESSION   DASTMD010000055 DASTMD010000000
VERSION     DASTMD010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165009"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1275)
                     /locus_tag="VFQ54_00845"
     CDS             complement(229..1275)
                     /locus_tag="VFQ54_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0163554.1"
                     /translation="MPYRESGERHFTRRTLVGTSIGTLAGLSVSRALASGTPPAVASP
                     ASPWTYEDVLGKKVSLPVRPSRIAANLVTAAALWDLGIRVVAVFDSTASAYPDGNHIA
                     WGNIDASAVTNVGDVDGNILPETLLTVRPDVILTLTFDRTDPSQINGVPGDLADAIER
                     IAPVLVVTDMDSTEIQLQRLVDLGAGLGADLEAAAIVESKAAYEAKVDEFRAVVTEKR
                     DLTVLFIDFDPGELYVGGPRGVSELQFLDNLGLPFANAESDAAGEFWETLSAEQALKY
                     PADVIYNDVYSGLRTEEELQTVAVYAAMPAIAAGQLGQWQRDFPVSYAGVADFLEVVL
                     ATLRTAEKVTSADS"
     gene            complement(1387..2385)
                     /locus_tag="VFQ54_00850"
     CDS             complement(1387..2385)
                     /locus_tag="VFQ54_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0163555.1"
                     /translation="MESTFPRMSVSRRRLLGGTLAAAAAVGISDRGTLAGPGTATPQA
                     ATPASSERSVSTPHGTIALPSTLDRIVCVDWFSPEILLELGTTPIAVPDFSGNNLILP
                     AYTDAVSDMPTITNTSLEVDLEAVAALKPDLILGLDWGAFHYEDLSRIAPTVLLKWNN
                     TSGDWPTFAAGFAEILGRQDQLASLRSKYLARANELKSTYSATEAKTQWVMIEGDDSG
                     QFYLYVQDYAGGVVLTDAGITLIPAAGGKTGTYVMVADEQVNELKDADVILIPADPTG
                     RPSPATEQLVGSAPFGALKAAKSGNIFAVKRGFPASYETGIAFLDELETVLKGLSS"
     gene            2730..4430
                     /locus_tag="VFQ54_00855"
     CDS             2730..4430
                     /locus_tag="VFQ54_00855"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU0163556.1"
                     /translation="MLTIPSDLEIAQSASMLPPADIAAQLGIDESWLLPYGRHVAKID
                     PAVADTMTDRQPAKYVVVTAITPTPLGEGKTTTSVGLAQGLSHIGYKSVLTLRQPSMG
                     PTFGIKGGAAGAGYSQVLPMEQLNLHLTGDLHAVTAAHNLLAAMVSNHLFQGNQLELD
                     PHGIIWRRVLDVNDRSLRSIVEGLGQKEDGIPRQTGFDITAASEVMTILSLATSAEDL
                     RKRLGRIVIGRNVRGEFVTAEDLKAAGAMAVILKDAIWPNLLQTTENTPVLIHAGPFG
                     NIATGNSSVIADQVAIRAGDFVITEAGFGSDMGAERFFNVKCRASGLKPDVAVLVVTV
                     RALKAHSGRFRIVPGRPLPATLLEESPDAVRAGAANLLKHIEIVKSFGVNPVIAINRF
                     ADDHDADIAEIETIAEEAGVRSAVATHVVDGGAGAAQLAQAVVDASLEPTDFRYTYEL
                     DASLECKLEAIATRIYGADGIDISPVAAGHLQTYAKAGFGDLPVLIAKTHLSISHDPK
                     LKGAPKGWTLPIREVRLAAGAGYVYAIAGDMRTMPGLGSSPAAERIDIDADGRIVGLF
                     "
     gene            complement(4687..>5676)
                     /locus_tag="VFQ54_00860"
     CDS             complement(4687..>5676)
                     /locus_tag="VFQ54_00860"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEU0163557.1"
                     /translation="WLVTVAWRKFLDIARADSSRRQREEILQVEPSSETGEDVDDTLQ
                     LYFLCAHPSLTPASAVALTLRAVGGLTTRQIARAYLVPEATMAQRISRAKRTVRDVRF
                     NQPGDVSTVLRVLYLVFNEGYSGDIDLADEAIRLARQLAARIDHEEVVGLLALMLLHH
                     ARRPARTAADGRLVPLAEQDRSLWNIRLIAEGVDVLQSALGRDRLGEYQAQAAIAALH
                     ADARTVDETDWAQIVEWYDELVRLTDSPVARLNRAVAVGEANGPRAGLAVLAELDPAL
                     PRYAAVAAYLHERDGDPVTAARLYAEAARSATSLAERDHLTRQAARLNAQLHH"
BASE COUNT          996 a         1800 c         1890 g          990 t
ORIGIN      
        1 gatacgcgat tgcgacgcaa ccggcaacat cgctcgtcgg caaactttgc gctggattcc
       61 tggggctgga tcagtgacgg gggtgattgg cgaaaaccat gcccgcagcc cgagatgcag
      121 gcgaggaggg acggaaacga cctgccaacc cggcgggccg tttccggaag tggtgccgtc
      181 ggtcgagcgc ctcgttgcgg ctctgcgaga tcgggccatc gggggcggct aggagtctgc
      241 gctcgtcact ttttcggcgg tgcgaagcgt cgccaacacg acctccagga agtcggccac
      301 gccggcgtag ctgacgggaa agtcccgttg ccattgcccc agctgacccg cggcaatcgc
      361 cggcatggcg gcgtagacag ccacggtctg aagttcctct tccgtgcgga gaccgctgta
      421 gacgtcgttg tagatgacat cggcggggta cttcagagcc tgttcggcgc tgagggtttc
      481 ccagaactcg ccggctgcat cggattccgc gttggcgaac ggcaggccga ggttgtcgag
      541 aaactgcagt tcggagacgc cgcgtgggcc tccgacgtac agctcgcccg gatcgaaatc
      601 gatgaagagc accgtgaggt cgcgtttctc ggtgacgacg gcgcggaatt catcgacctt
      661 tgcctcatag gcggccttcg actcaacgat cgccgcggct tcgagatcgg cacccagccc
      721 cgcgccgaga tccaccagac gttggagctg gatctccgtc gaatccatgt cggtcacgac
      781 gagtacgggc gcaatccgct cgatggcatc ggccaggtcg cccggcacgc cgttgatctg
      841 ggaagggtcg gtgcggtcga acgtcagggt caggatgacg tccggccgga ctgtgaggag
      901 cgtctccgga aggatattgc cgtcgacatc gccgacgttc gtcacggccg aggcgtcaat
      961 gttgccccag gcaatgtggt tgccatccgg atatgcggac gccgtcgaat cgaagaccgc
     1021 cacgacgcga attccgagat cccagagggc ggcggcggtg acgagatttg ccgcgatgcg
     1081 cgatggccgg accggcagcg agaccttctt gccgagcacg tcctcatagg tccagggcga
     1141 cgccggactg gccaccgccg gaggggtgcc acttgccagc gcccgtgaaa cggaaagccc
     1201 cgcaagggtg ccgatcgatg tcccgaccag agttcttcgc gtgaagtgcc gttctccgga
     1261 ttcccgatag ggcattggtg agacctccat gatggcgacg cgatgccccg aggcgtcgcg
     1321 ccgcgtattc ggatttcgcc ggcggcgtga cctacgcccg gcttcgtcgc cgggattgac
     1381 tcgtcgctat gaggagagtc ccttcagcac ggtctcgagc tcatcgagga aggcaatgcc
     1441 ggtctcgtaa ctcgccggga atccgcgttt gaccgcgaaa atgtttcccg attttgccgc
     1501 cttcaacgcg ccgaatggcg ctgatccgac gagctgttcc gtcgcgggcg agggacgtcc
     1561 ggtcggatcg gcaggaatga ggatgacgtc cgcgtccttc agttcgttga cctgttcgtc
     1621 ggccaccatg acgtacgtgc cggtcttccc cccggccgcc gggatgagcg tgatccccgc
     1681 gtctgtcagg accaccccgc ccgcgtagtc ctgaacgtag agatagaact ggccggagtc
     1741 gtcaccttcg atcatcaccc actgcgtctt tgcttccgtc gcgctgtagg tggacttcaa
     1801 ctcattcgcc cgcgcgaggt acttcgatct caacgatgcc agctgatcct ggcgcccgag
     1861 aatctccgcg aacccggcgg cgaacgtcgg ccagtcgccc gaggtgttgt tccacttcag
     1921 gagaacggtt ggcgcgatcc gggagagatc ctcgtaatgg aatgcacccc agtcgagacc
     1981 gagaatgaga tccggcttga gcgccgccac cgcctcgaga tcgacctcga gtgacgtgtt
     2041 cgtgatcgtc ggcatgtcgc tgacggcatc cgtataggcc ggaagaatca ggttgttccc
     2101 gctgaagtcc gggacggcga tgggcgtcgt tccgagttcg aggagaatct ccggactgaa
     2161 ccagtccacg cagacgatgc ggtcgagcgt ggatgggagt gcgatcgtgc cgtggggggt
     2221 ggaaacgctc cgctcggaac tcgcgggcgt tgcggcctgc ggagttgccg tacccggtcc
     2281 ggccaacgtg ccgcggtcgc tgatcccaac cgccgccgcg gctgccaagg tcccgccaag
     2341 caatcgccgg cgcgagacac tcattctggg gaaggtactt tccatgcgca ttctccttgc
     2401 agtgttcgcg ttgccgtcat catgaagaac ccattccggc tgagttcgag cccaaataaa
     2461 gccgactatg aaagtcggat atcaatatag ggtgaatcca gacttttcac aatcagcgaa
     2521 tggtgcgcgc ggggcgagaa cggtggtgca tgagcgtttg gccgaaacga cccggccttc
     2581 cggctcgcgg gattgagccg cactgaactg ccgacgtcat tctcgtgtcc ggatatcttg
     2641 cgccgcgccg caggattcgg cattgttcac aggttgcgcg cgttcatcgc cgctcgcctt
     2701 cgatcgtcat tcgcaccagg tagaggttca tgctcacaat cccgtcggat cttgaaatag
     2761 cgcagtctgc ttcgatgctg ccaccggcag acatcgccgc ccagctcggc atcgatgaat
     2821 catggcttct tccctacggt cgccacgtcg cgaagatcga tcccgcggtc gcggacacca
     2881 tgaccgaccg ccagccagcc aaatatgtgg tcgtcacggc gatcacgccg acgccgctgg
     2941 gcgaaggcaa gaccaccacc tcggtcggtc tcgcgcaggg gctctcgcac atcggataca
     3001 agtccgtcct caccttgcgt cagccatcga tgggccccac cttcgggatc aagggcggag
     3061 ccgccggcgc cggctactcc caggtcctcc cgatggaaca gttgaacctc catctcaccg
     3121 gtgatttaca cgccgttacc gcggcgcaca atctgctcgc ggcgatggtc agcaaccatc
     3181 tgttccaggg aaaccaactc gagctcgatc cccacgggat catctggagg cgggtgctgg
     3241 acgtgaatga ccgatccctg cgctcgatcg tggaggggct cggccagaag gaagacggca
     3301 ttccccggca gaccgggttc gacatcaccg ccgcgtccga agtgatgacc atcctctccc
     3361 tcgccacctc ggcggaagac ctgcggaagc ggctcgggcg gatcgtgatt ggccgcaacg
     3421 tgcgcggcga gttcgtgacg gcggaggacc tgaaagcggc cggcgcgatg gccgtgatcc
     3481 tgaaagacgc aatctggccc aatctgctgc agaccacgga aaacactcca gtgctcatcc
     3541 acgccggacc gttcggcaac atcgcgacgg gcaattcctc ggtgattgcg gaccaggtgg
     3601 caattcgcgc tggcgacttc gtcatcacgg aggcagggtt cgggtccgac atgggggccg
     3661 agcgattctt caacgtgaaa tgccgcgcct cgggactcaa gccggatgtc gcggtgttgg
     3721 tggtgacggt ccgcgcgctg aaggcgcact cgggtcgctt ccggatcgtt cccggacgac
     3781 cgctgccggc cacgctgctg gaggagtcgc ccgatgccgt acgggccgga gcggccaatc
     3841 tgctgaaaca catcgagatc gtgaagtcgt tcggggtcaa tccggtcatc gcgatcaacc
     3901 gattcgcaga tgaccacgac gccgatatcg cggagatcga gacgatagcg gaagaagccg
     3961 gcgtgcgctc tgccgtagct acccacgttg tcgatggagg cgcgggtgcc gctcaactcg
     4021 cccaggcggt ggtcgatgcc agccttgagc cgacggattt tcgctatacc tacgagcttg
     4081 acgcctcgct ggaatgcaag ctggaggcga ttgcgacgcg aatctacgga gctgacggca
     4141 tcgatatctc gccggttgcg gcgggtcatc ttcagacgta tgcgaaggcc ggattcggcg
     4201 atctgcccgt gctgatcgcc aagacgcatc tatcgatcag ccacgatccg aagctgaagg
     4261 gtgcgccgaa aggctggacg ctgccgatcc gcgaagtgcg gctcgccgcc ggggccgggt
     4321 acgtctacgc gatcgccgga gacatgcgca cgatgccggg tctcggttcg tcgccggcgg
     4381 cggaacgcat cgatatcgac gccgacggca ggatcgtcgg tcttttctag gaaggaccag
     4441 aacggcgcga cggggagcac gatccgtggg cgaaccgaag gcgggacagt cgtccggacc
     4501 tccggtccgg cagcggcggt cccgctcctt cgacaagctc aggacaagct cccgaacgac
     4561 cgggcgcacc gcggaatatc gcattcggtc cggcaggacg ttcggcaacc gttttggatt
     4621 tcgctacagg aaacgccggg gcggcgccca tggtgcggcc gaagcgcgcc cgctacggtg
     4681 gggtcctcag tggtgcagct gcgcgttgag ccgcgcagcc tggcgcgtga ggtgatcgcg
     4741 ttcggcgagg ctggtcgccg atcgggcagc ttccgcgtag agccgtgctg cggtcaccgg
     4801 gtcgccgtcg cgctcgtgga gatacgccgc gacggcggcg tatcggggga gggcggggtc
     4861 gagttccgcc agcacggcca ggccggctcg cgggccgttc gcctcgccga ccgcgacggc
     4921 ccggttgaga cgggccaccg ggctgtcggt gaggcggacg agttcgtcgt accactcgac
     4981 gatctgcgcc caatcggtct cgtcgaccgt ccgcgcgtcg gcgtggagcg cggcgatcgc
     5041 ggcctgggcc tggtactcac ccaggcgatc gcgaccgagg gccgactgga gcacgtcgac
     5101 gccctcggcg atgaggcgga tgttccacag gctgcggtcc tgctcggcga gcggcaccag
     5161 tctgccgtcg gcggccgtcc gtgccgggcg ccgcgcgtgg tggagcagca tcagcgcgag
     5221 cagacccacc acttcctcat gatcgatccg ggcggccagc tggcgagcaa gccggatcgc
     5281 ctcatccgcc agatcgatgt cgccggaata gccctcgttg aagaccaggt agagcacgcg
     5341 cagcaccgtg gagacatcac cgggctggtt gaaccgaaca tcccggacgg tccgcttggc
     5401 cctgctgatt cgctgggcca tggtcgcctc gggcaccagg taggcccgcg cgatctgacg
     5461 cgttgtcagc ccacccaccg cgcgcagcgt gagcgcgacc gccgacgccg gtgtcaggga
     5521 cgggtgcgcg cagaggaagt agagctgaag ggtgtcgtcc acatcctcgc ccgtctccga
     5581 cgacggctcg acctggagga tttcctcgcg ttgccgtcgg gaggaatcgg cgcgagcgat
     5641 gtcgaggaac ttccgccagg ccacggtgac cagcca
//
LOCUS       DASTMD010000056         1592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3040848, whole genome shotgun sequence.
ACCESSION   DASTMD010000056 DASTMD010000000
VERSION     DASTMD010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3040848"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFQ54_00865"
     CDS             <1..194
                     /locus_tag="VFQ54_00865"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEU0163558.1"
                     /translation="LLERTDNYTYCPYKGDCSYYSVPAGGDAAVNAVWTYETPYDAVS
                     EIKEYLAFYPNRVEISEEA"
     gene            777..1544
                     /locus_tag="VFQ54_00870"
     CDS             777..1544
                     /locus_tag="VFQ54_00870"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEU0163559.1"
                     /translation="MTDTITPASATTNALPPIVSREEWKEARVSLIEKETELGKLMAE
                     VAAIRRRQPMVEITKTYTFDGPEGPVSLLDLFEGRRQLIVYHFMFHPDWDEGCVGCSM
                     MLDHFGHPSHLHARDTSRVVVSRAPLAKLLAHKQRMGWTEPWYSSFGTDFNADFDVTI
                     DGDEEIPATSVFLRDGDRIFQTYRDSDATGDAVMNTFTFLDLTPFGRQEIGEDSPAGW
                     PQTSGPWEWWRHHDRYDGAAAPSSCGCGEGCSCGEAD"
BASE COUNT          294 a          513 c          484 g          301 t
ORIGIN      
        1 cgctgctcga gcgaaccgac aactacacgt actgcccgta caagggagac tgctcctact
       61 acagtgttcc ggccggtggt gatgccgccg tgaacgcggt ctggacctac gagacgccct
      121 acgacgccgt ctccgagatc aaggagtacc tggccttcta cccgaaccgg gtcgagatct
      181 ccgaagaggc ctaatcgtcc aagtgggttt gcgacgggat ccgttcgacg cgcatttggc
      241 gggacataaa agtcccgccg tactcacgga cgctgcacat acactggtcg ttcgtagggc
      301 aggacctccg tgtcctgcca atccaggtcg ttcgatgccc catgcgctcc cgataacgac
      361 ggtttcgacc gtcgccaccg ggagcacgac ccggggggcg aatgcgaggc gagacaccgc
      421 attcgatctc aggcatgtgg caaccgtttc ggttctcgcc ctgatgcaag acgcttcgcc
      481 gtgccgcctc atgatcgtcc cgtcctcacg gcccttgtgg ccgttccgtt ccgggtcgat
      541 gcggctacgc cgatcccgtg gcgtggcaga gaaagccttg cgcgatacct tgatgtcgcg
      601 gcgagagaca aactctcgcc ccgcggatgt gtttgtccgg tcgacatgag acggcgtccg
      661 acgacaggcc gttgtcagcg caatcaggaa gaagcgatta cgaacccctc tccctaccat
      721 ggaagcacgt tatgccccga ctcgttgagg ggtgttccat ggaaagggtc tcacccatga
      781 ctgacaccat cacgccggct tcggccacca ccaacgcgct gccaccgatc gtctcccgcg
      841 aggaatggaa ggaagcccgc gtctctctca tcgaaaagga aacagagctt ggcaagctca
      901 tggccgaggt cgcggccata cgtcgccgcc agccgatggt cgagatcacg aagacctaca
      961 ccttcgacgg tccggaagga ccggtctccc tcctcgatct tttcgagggt cgtcgccagc
     1021 tgatcgtcta ccacttcatg ttccacccgg actgggatga gggctgcgtc ggctgctcga
     1081 tgatgctcga tcatttcggg cacccatccc accttcatgc gcgcgacacc tcgcgcgtgg
     1141 tcgtttcacg cgctccgctc gcgaagttgc tggcccacaa gcagcggatg ggttggacgg
     1201 agccctggta ctcctcgttc ggcaccgatt tcaatgccga cttcgacgtg acgatcgatg
     1261 gcgacgaaga gattcccgcc accagcgtct tcctgcgcga tggggatcgg atcttccaga
     1321 cctaccgcga tagcgacgcg actggtgacg cggtcatgaa cacgttcacc ttcctcgacc
     1381 tgaccccgtt cgggcggcag gagatcggcg aggattcccc ggccggctgg ccgcagacat
     1441 cgggaccctg ggagtggtgg cgccatcacg accgatacga cggtgctgct gcgccatcga
     1501 gctgtgggtg tggcgaaggc tgctcgtgtg gtgaggccga ctagttctcg tcgaaacgcg
     1561 aggcattggg caagcgtgga ggcgatcccg gg
//
LOCUS       DASTMD010000057         1774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_808770, whole genome shotgun sequence.
ACCESSION   DASTMD010000057 DASTMD010000000
VERSION     DASTMD010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808770"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(413..1357)
                     /gene="thyX"
                     /locus_tag="VFQ54_00875"
     CDS             complement(413..1357)
                     /gene="thyX"
                     /locus_tag="VFQ54_00875"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEU0163560.1"
                     /translation="MTTATLTEVESSPAYERVRVPELDAILGQQFKVLDDGFVRVVDY
                     MGGDSSIVQAARVSYGAGTKKVREDRGLIRYLIRHQHTTPLEMCELKLHVRVPMDTWR
                     QWVRHRTASVNEYSTRYSIAIDAAQQTPEDAWRGQSASNRQGSEGWIPTETGTKLTAR
                     EAELHRLSREIYDERLELGVAREQARKDLPLATYTEAYWKVNLHNLMHFLALRMDAHA
                     QFEIREYASTIGNEIVSRWCPVAWEAFLDYNTNGLSFTTSELAIVQALTAGDVAAAIG
                     EATQHGFITVKDGVTRVSREGKELGPKLARLGLTAPWE"
     gene            1500..>1774
                     /locus_tag="VFQ54_00880"
     CDS             1500..>1774
                     /locus_tag="VFQ54_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163561.1"
                     /translation="MAENRGILDRAARAPDLTIAYGSHADHIADVWLPQDSRATSVPL
                     ILLWHGGFWQQEWDRMHLAPMAEACAALGYAVALPEYRRAGGDGGWPT"
BASE COUNT          295 a          515 c          623 g          341 t
ORIGIN      
        1 agggaatccc gaaccccgcg ctcgtgatcg tcgtcgccga ggggttgtcg cgcattgacg
       61 ccgaatccga ggcgagcggg ccgctaagga aacatgatgg acaatccccc accgtgtgac
      121 cggatgcgaa acgcatccgc aggtcaggga cgacttggtg aggatagctt cgcttgtccg
      181 ccagagcaca aggatacgtt agctcgtgaa acggtaaact gcccggcgga atgcgctccc
      241 cgtcgggcag tttgaattct tggagctggg ttcagggcgg tggcgacacc ggtgtcgcgc
      301 gccacgcagg cgatcctgtt tgcggcaagg cacaaggcct tgccctacag accccggttt
      361 gtccgcaacg tggcgagcga tccgaactgc gctgtaaggc tcgagtgggc cactattccc
      421 agggagcggt caggccgagg cgagcgagct tcgggccgag ttccttgcct tcgcgactga
      481 ctctggtgac gccgtccttc accgtgatga agccgtgctg cgttgcttcg ccgatcgccg
      541 cggcgacatc tcccgccgtg agcgcctgaa cgatcgccag ttctgacgtc gtgaacgaga
      601 ggccgttcgt gttgtagtcg aggaatgcct cccaggcgac cgggcaccag cgagagacga
      661 tctcgttgcc gatcgtcgag gcgtactccc ggatctcgaa ctgggcgtgg gcatccatcc
      721 gcagcgcgag gaagtgcatg aggttgtgca gattgacctt ccagtacgcc tcggtatagg
      781 tggcgagcgg gagatccttg cgtgcctgtt cgcgggcgac gccgagctcg agtcgctcgt
      841 catagatctc gcgggagagg cgatgcagct ccgcctcgcg ggcggtcagc ttcgtgccgg
      901 tctcggtggg aatccaccct tcgctgccct gacggttgct tgccgattgt ccgcgccagg
      961 cgtcttcggg cgtctgctgc gcggcatcga tcgcgatcga gtagcgcgtc gagtattcgt
     1021 tgacgctggc cgtgcgatgg cgcacccact ggcgccaggt gtccatcggc acgcggacgt
     1081 ggagtttcaa ctcgcacatt tcgaggggag tcgtgtgctg atggcggatc agatagcgga
     1141 tcaggccgcg atcctcgcgg accttcttcg tgccggcgcc gtaggagacc cgggcggcct
     1201 ggacgatcga gctgtctcca cccatgtagt cgacgacgcg aacgaagcca tcatcgagca
     1261 ccttgaattg ctggccgaga atggcatcga gttcgggaac gcggacgcgc tcgtaggccg
     1321 ggctgctttc gacttcggtg agggttgctg tcgtcacggt cgtgtccttg tggtgtgatt
     1381 cggatcgagg tcgtggttgg ggacgacggg cgaacgatcc ggaacctgtc gtctcgacca
     1441 cgaagcgtac gtacagtatg ccataggcac gaatcgagct tggaagggga ggcgggaaga
     1501 tggcggagaa tcgcgggatt ctggatcgag cggcgcgggc gcccgatctg acgatagcgt
     1561 atggatcaca tgccgaccac atcgccgacg tctggctgcc gcaggattcg cgtgcaacct
     1621 cggttccatt gatccttctc tggcatggcg gcttctggca gcaggagtgg gatcggatgc
     1681 atctcgcgcc gatggcggag gcgtgtgcag cgcttgggta cgcggtggcg ctgccggagt
     1741 accggcgtgc gggcggggac ggcggctggc cgac
//
LOCUS       DASTMD010000058         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_183920, whole genome shotgun sequence.
ACCESSION   DASTMD010000058 DASTMD010000000
VERSION     DASTMD010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183920"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(426..1517)
                     /locus_tag="VFQ54_00885"
     CDS             complement(426..1517)
                     /locus_tag="VFQ54_00885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU0163562.1"
                     /translation="MSGEDYEAGRTRFDMTGCEGSGADVIDDYQHHRLVTFVAPIAPW
                     VMVGAAGMCATVGIGPFLFGVHSNAGAVGAILLTATTLVALAGILRYTASHRHLDRSC
                     FCGMVYLLVLSSGLSISFHEHGDGVSPALPFVVLASVVSTVFWLRVWHMVAGLIACFA
                     PPMILMATTTATTTDWFRGVQLVVPTALACIGLYFLLQRANEQLFDANARLQERAMRD
                     GLTGLLVRTVWLEYAEEAVSRNRLLQRATALIFIDLDNFKTVNDRHGHATGDLLLERA
                     GRLLRRHAPPRALTGRFGGDEFVVLLTDVDGDAGERYVEMMRGVFDDRDLPISATFGL
                     AVSLGDAALHELMHTADMQMLGQKIGSRR"
     gene            1701..>3120
                     /locus_tag="VFQ54_00890"
     CDS             1701..>3120
                     /locus_tag="VFQ54_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0163563.1"
                     /translation="MKPDQTSLPRLSRRRFTQSAAAIAAAGLTPALLAHQAGAQDATP
                     DAGASPVAATPDPNASFKLDSVSRADYLTQLKDHYKFEAPKQTGGDIIQVFTTDLATV
                     NPILAQDLAAGYMIGLVFEALVQINPIDGTPAPSLADSWEISSDGLRYRFALNPKATW
                     HDGQPVTADDVIYTYDAVLDPTSLSPTRATLLAILADYKKIDDHTVELTARGQIATFL
                     TQAANVTVMAKHIWEGVAFKDWTSDPGTTGQDPKRVIGSGPFTFVEWVQADHCTVAKN
                     ANYWLPDQVPSIDHFIYRVVADASSSTQSIQSGESDIVGLSAAQAPGFIKANPDLNIT
                     TFDQTHMYYYITNLDGTKLPFFTDKRVRQAMMWALDRDLVAKQIFTGYAVRSDGSQPP
                     ISPGYAPDKITSIYTYDPDKAKSLLDAAGWTAGSDGIRSKDGKRFSVEILYTNSSVQN
                     QQLIPYMQQCWKDVGIELTSSAM"
BASE COUNT          614 a         1111 c          912 g          483 t
ORIGIN      
        1 gagcgccggc gtgcctccta cggtgatcga gtcagccttg tcggcccatg gagactggtc
       61 aggagttgct tgagaaggcc cggtgcagcg aatacatccc agagaatgac cacgtcgcga
      121 ccaacgtctc acctggatcg ccgcgggtgc ggaaccggtc atgcttggct catgggcggc
      181 cgccactcat ccgcggtcac gacttagcgg acgcgccacc taacagcttg gcaaaagtct
      241 cgccgcattc cgtcgagagg ctacgccccg cccgcaaggc gaatatgcgc cgcaaaaaac
      301 cgatgaggct tttgccaacc cgcccgacaa accccggcgg ttccaacggc gttccatgca
      361 tgacaaccgc cggatcgcga ccgaagctcg atgcggtcgt gatccggatc atgccgggcg
      421 gtgtcctatc gccggctgcc gatcttctgg ccgagcatct gcatgtccgc ggtgtgcatc
      481 agctcgtgca acgccgcgtc acccaggctt accgcaaggc cgaaagtcgc ggatatcgga
      541 agatcacgat cgtcgaagac tccgcgcatc atctcgacat accgctcgcc agcatcgccg
      601 tcgacgtccg tcaggagcac gacgaattcg tcaccgccaa agcgccccgt caacgccctc
      661 ggcggggcat gcctccggag caatctcccg gcacgctcca ggaggaggtc ccccgtcgca
      721 tgtccatgcc ggtcgttgac cgtcttgaaa ttgtcgaggt cgatgaagat caacgcggtc
      781 gcccgctgca gcaggcgatt gcgcgatacc gcctcctcgg cgtattcgag ccagaccgtc
      841 cggacgagaa gcccggtcag gccatcgcgc atcgcgcgct cctgcagccg cgcgttggca
      901 tcgaagagct gttcgttcgc gcgctgaagc aggaagtaga gcccgataca ggcaagcgcg
      961 gtcggcacga ccagttgaac gccacgaaac caatccgtcg tcgtcgcggt cgtcgtcgcc
     1021 atcaggatca tcggcggcgc gaagcaggca atcagtcccg cgaccatgtg ccagacgcgc
     1081 agccagaaaa ccgtgctcac cactgaggcc agcacgacga acggcagtgc cggggagacg
     1141 ccgtcgccat gttcgtggaa ggagatgctg aggccgctgc tgaggaccag gagatagacc
     1201 atgccacaga agcaggaacg atcgagatgg cggtgactcg ccgtgtagcg gaggatcccc
     1261 gcgagggcga caagcgtcgt cgccgtgagg aggatggccc cgacggcccc ggcattcgaa
     1321 tggactccga agagaaacgg accaatcccg accgtggcgc acatgccggc ggcgccgacc
     1381 atgacccacg gcgcgatagg cgcgacgaat gtcacgaggc ggtgatgttg atagtcgtcg
     1441 atcacgtcgg ctcccgaccc ctcgcatccc gtcatgtcga atctggtgcg ccctgcttca
     1501 taatcctcac cggacatcct accctcccgg ggaaccgtag agcaaagaag ccgcctcgcc
     1561 cggcgtcgcg ggcgcgccac cgccttcctc tccggccgaa tccccggtac gatgcgaccg
     1621 ggcaacacag gctcgctccg gatgactccc gcctcgagct tcaggcggcg agttcacgat
     1681 tcaggaacaa ggggaccccc atgaagccag accagacatc attgccacga ctctcgcgcc
     1741 gccggttcac ccaatccgcc gccgcgatcg ccgccgccgg cctcacgccg gccctcctcg
     1801 cacaccaggc cggcgcgcag gacgccaccc cggatgcggg cgcctccccg gtcgcggcga
     1861 cgccggatcc gaatgcgagc ttcaagctcg attccgtctc ccgcgccgat tacctcaccc
     1921 agctcaagga ccactacaag ttcgaggcgc cgaagcagac cggtggcgac atcatccagg
     1981 tcttcacgac cgacctcgcg accgtcaatc cgatcctcgc gcaggatctc gccgccgggt
     2041 acatgatcgg tctcgtcttc gaggcgctcg tccagatcaa cccgatcgac ggcaccccgg
     2101 cgccgtccct cgccgactcc tgggagatca gcagcgacgg tcttcgctac cggttcgccc
     2161 tgaatccgaa ggcgacctgg catgacggcc agcccgtcac cgccgacgat gtcatctata
     2221 cctacgatgc cgttctcgac ccgaccagcc tcagcccgac ccgcgccacg ctgctcgcga
     2281 tcctcgccga ttacaagaag atcgacgatc acaccgtcga gctgacggca cgcggccaga
     2341 tcgcgacctt cctcacccag gccgccaacg tcaccgtgat ggccaagcac atctgggaag
     2401 gcgtcgcctt caaggactgg acgtccgatc ccgggacgac cgggcaggac ccgaagcgcg
     2461 tgatcggctc cggccccttc accttcgtcg agtgggtgca ggcggatcac tgcaccgtcg
     2521 cgaagaacgc gaactactgg ctgccggacc aggtgccatc gatcgaccac ttcatctacc
     2581 gcgtggtcgc cgacgcctcg agttccacgc agtcgatcca gtccggggaa tcggacatcg
     2641 tcggtctctc cgcggcacag gcgcccggct tcatcaaggc caatcccgat ctcaacatca
     2701 ccaccttcga ccagacgcac atgtactact acatcacgaa cctggacggc acgaagctcc
     2761 cgttcttcac ggacaagcgc gtgcgccagg cgatgatgtg ggcgctcgat cgcgatctcg
     2821 tcgccaagca gatcttcacg ggctacgcgg tccgctccga cggatcgcag ccgccgatct
     2881 cgccgggcta cgcgccggac aagatcacca gcatctacac ctacgatcca gacaaggcga
     2941 agtccctcct cgatgccgcc ggctggacgg cgggatcgga cgggatccgc tccaaggatg
     3001 gcaagcgatt cagcgtcgag atcctctaca ccaactcgtc cgtccagaat cagcagctga
     3061 ttccctacat gcagcagtgc tggaaggacg tcggtatcga gctgacctcc tccgcgatgc
//
LOCUS       DASTMD010000059         1585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1879835, whole genome shotgun sequence.
ACCESSION   DASTMD010000059 DASTMD010000000
VERSION     DASTMD010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879835"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFQ54_00895"
     CDS             <1..406
                     /locus_tag="VFQ54_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163564.1"
                     /translation="GDAAAARIAPSTFVLPTVSPPEIDFELWTETIARMRAVAPERLA
                     LTHFGVFDDVDRHLDAVLPNSERELGIVRVALQAGLSDEDATTRLLDAERSELNGNQV
                     GGGVLAQMELAMPAWLATLGLRRVLRKRGEIE"
     gene            complement(771..1286)
                     /locus_tag="VFQ54_00900"
     CDS             complement(771..1286)
                     /locus_tag="VFQ54_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HEU0163565.1"
                     /translation="MPYHFDPFARETDDYEDDAFDPFFERTSARASRPRVERTEEASP
                     RRKSRTQRPPRTEADQAFKCGHCKQFIGAPISGGRQRNHCPNCLYSKHVDDTMPGDRK
                     SDCHSLMAPLGIISRRNGEEQILHRCLGCGKIQPNRIAADDHPVLLEELPHVSLSDYV
                     SAEEQDAPIEP"
BASE COUNT          242 a          521 c          546 g          276 t
ORIGIN      
        1 gggcgatgcc gcggcggcac ggatcgcgcc gtcgacgttc gtgctgccga ccgtctcccc
       61 gccggaaatc gacttcgagc tctggacgga gacgatcgca cgaatgcgcg cggtcgctcc
      121 cgagcggctc gcgctgaccc atttcggtgt cttcgacgat gtagatcgcc acctcgacgc
      181 cgtgctgccg aactcggagc gcgaactcgg catcgttcgg gtggcgctcc aggccgggct
      241 ttcggatgag gacgcgacga ctcgactcct cgatgcggag cggtcggagc tgaacggcaa
      301 tcaggtcggc ggcggcgttc tcgcccagat ggaactggcg atgcccgcct ggctcgcgac
      361 gctcggtctt cgccgcgtcc tcaggaagcg cggtgagatc gagtaagccg tccggacccg
      421 ccatctgccc cggcaggacg ccgctgccgg gaaccgggag gcgggacatt actccatcca
      481 gagacccggc ctggctctgc acaggcgggt cgttcgccgg tctccggatt ccgcagtccc
      541 gccctacgaa cgaccgcggc gccgacgatc gcgcatgatg ttcgccatgc gttgaacgcc
      601 tcgaacctcc gtggcgggtc accccgtgtg acccatccgg cggagccgga tatggacgtc
      661 cgcctcgcga tggcgacggc acggaaccgc ggcgccgcgg cgtcctcgcc gcccgacgac
      721 gcgcgacccg gagcgcatcc gtcgcgcgaa caggcccgcg gtccggagcc tcaaggctcg
      781 atgggcgcgt cctgctcctc ggccgagaca tagtccgaga ggctcacgtg ggggagttcc
      841 tcgagcagga cgggatgatc gtccgccgcg atgcgattcg gctggatctt cccgcagccg
      901 aggcagcggt ggaggatctg ctcctcaccg ttgcgacggc tgatgatccc caacggagcc
      961 atcagtgagt ggcaatcgct cttgcgatcg cccggcatcg tgtcgtccac gtgcttcgag
     1021 tagaggcagt tcgggcaatg attgcgctgg cgaccgccgc tgatcggggc gccgatgaac
     1081 tgcttgcaat ggccacactt gaacgcctgg tctgcttccg tgcgcggtgg ccgttgcgtc
     1141 cggctcttgc ggcggggcga ggcttcctcg gtgcgctcga cgcgcgggcg gctcgcccgt
     1201 gccgacgtcc gctcgaagaa gggatcgaag gcgtcatctt cgtagtcatc ggtttcgcga
     1261 gcaaatggat cgaaatggta cggcacccgt gattctccgg cgcgatgtgc gcgagtcact
     1321 cttcatgaca gcagtggtga atggctttgc cgtcgcggtc gcgatcggac ttcggggatg
     1381 gcgggcgagg cacgtgtgac cggaaacgtc tcgagctgct tctcgccgga tacgacgaaa
     1441 tgccgccctc gcgggcagtt ggctcaaggt tcttcccggc acctgatgcg tcagcgatcg
     1501 tgctagacga tgcgtcccgc cacacgtgca aggatcaggg agtgccgcat gggacggctc
     1561 gtttccaggt cagcatcgct gacgg
//
LOCUS       DASTMD010000060         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2951931, whole genome shotgun sequence.
ACCESSION   DASTMD010000060 DASTMD010000000
VERSION     DASTMD010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2951931"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFQ54_00905"
     CDS             complement(<1..366)
                     /locus_tag="VFQ54_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163566.1"
                     /translation="MAIGTVGGTVDAMLVIATGRHRRSVSSPFDSFRGDPMRRRTEKR
                     LPDFDYTAPGPYWVTIKLHRPQPLFGSVHDDGVHLSDIGEMIDAEWQNLPSRYPSVAL
                     DAYVVMPDHIHGLLTLFESP"
     gene            complement(495..1070)
                     /locus_tag="VFQ54_00910"
     CDS             complement(495..1070)
                     /locus_tag="VFQ54_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163567.1"
                     /translation="MTRVYRLRTSLLVALLALGFTLGVAISPASASRTLAIAAATPGT
                     PVAQTSANPKVGDAVDYINESGGVIATLTVSKVVQPWDEYGDYYAPDPGSEYVAFAID
                     IEHFGTRGDLLISPDDFYLQDVDGFLLSRSWSAGADDAKLKPTTDPIAISPGKTTEVV
                     IVFEVLQGVDLSDLYWQPDFDRLITLANLRS"
     gene            complement(1266..1814)
                     /locus_tag="VFQ54_00915"
     CDS             complement(1266..1814)
                     /locus_tag="VFQ54_00915"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEU0163568.1"
                     /translation="MRKLLVALFAMLFMVGGSTGFAAAQGTPATPVAGQGGAGNPANP
                     QIGDTVTYFGTNGTSIGSLKVTKITRGWDSYQQYEDPKAGDEYVAVYLTVTNTASRGT
                     LEIQAYNFALQDTQGFEISTAYAQGRDSLKTKPLTDTLEIPAGKSVNTLLVFEVYQDQ
                     PLQALYWMPDGYLINLADLSKS"
BASE COUNT          406 a          647 c          669 g          409 t
ORIGIN      
        1 tgggctctcg aacaacgtca agagtccgtg gatgtgatcc ggcatcacga catatgcatc
       61 gagtgcaacg cttggataac gggatgggag attctgccat tccgcatcga tcatctcacc
      121 gatgtcggag aggtgaacgc cgtcgtcgtg cacggatccg aatagtggct gcggtctgtg
      181 aagcttgatc gtaacccaat agggtccggg ggccgtgtag tcgaagtccg gcaggcgttt
      241 ctcggtacgt cggcgcattg ggtcccctcg gaaagaatcg aacggcgacg agacggaccg
      301 ccggtggcgg cccgtggcga tgacaagcat cgcgtctacg gttcctccaa cggtgccgat
      361 cgccaagcac cgcgttcgtg tcgacggttc atccaacgag gccgattgcc aggcgtcgcg
      421 ttggcagttc atcccatctc gttccgtgag gagcctttcc tccgacgccc gacgcgccgc
      481 caggcccgtt tgcgtcagga gcggaggttg gcgagggtga tcaggcgatc gaaatcgggc
      541 tgccagtaga ggtcgctcag atcgactccc tgcaggactt cgaagacgat caccacctcg
      601 gtcgtcttgc ccggcgagat cgcgatcgga tcggtcgtcg gcttcaactt cgcgtcgtcg
      661 gcgccagccg accaggaacg cgagagcagg aaaccgtcga cgtcctgaag gtagaagtca
      721 tcaggggaga tgagcaggtc gccgcgggtg ccgaaatgct cgatgtcgat cgcaaaggcg
      781 acgtattcgc tgcccgggtc gggcgcgtag tagtccccgt attcatccca cggctggacg
      841 accttcgaga cggtcagcgt cgcaatgacg ccgccactct cgttgatgta gtcgacagca
      901 tccccgacct tcggattggc cgaggtctgg gcgacgggcg tacccggagt cgcggcggcg
      961 atggccaacg tacggctcgc gctcgccgga gatatcgcca cgcccagcgt gaaaccgagc
     1021 gccagcagcg cgaccaggag agatgtccgc aaacggtaaa ctcgtgtcat tgtcaatcct
     1081 gcgtcgaatc gtccgcttct ggttcaagaa ccccgctata gccccggatc attgaaacag
     1141 gaacgtggcg atcgagccac tggttccggg attcgatcgc acgagaaacg aatgcgaccc
     1201 accccggcag agcggagtgg gtcgtttcgc ttccccacgg ggaagccaag cggcaaacgt
     1261 gatgcctagc tcttggagag gtcggcgagg ttgatgaggt agccgtccgg catccagtaa
     1321 agcgcctgca gcggctgatc ctgatagacc tcgaagacca gcaacgtgtt gacggacttg
     1381 cccgccggga tttcgagagt atcggtaagc ggcttggtct tcagtgaatc gcggccctgg
     1441 gcataggcgg tcgagatttc gaacccctgg gtgtcctgga gcgcgaagtt gtaggcctgg
     1501 atctcgagcg ttccgcggga ggcagtgttg gtgacggtga ggtaaaccgc cacgtactca
     1561 tcgccggcct tcggatcctc gtactgctgg tagctgtccc agccgcgcgt gatcttggtc
     1621 accttcagcg agccgatgga tgtgccgttg gtcccgaaat aggtgacggt atcgccgatc
     1681 tggggattgg ccggattacc ggcgcctccc tgcccggcga ccggcgtcgc cggcgtcccc
     1741 tgggcggcgg caaatccggt cgatccaccg accatgaaga gcatggcaaa caacgcgaca
     1801 agcagctttc gcataggaat ccctccgttg ttgtgtcccg gaaccagatg actctcccac
     1861 gagtcacagg ttcgcctttc gaaagccctc ccccttggag ggcccaagcg acggacggca
     1921 cgcctttcgc cgcacggtca ccgaagcggt ggtcggcagc ccgccaacac tccgttctcc
     1981 tccatcaacg acacagcaat cctgacggtt ccatgccctg gcgatgcctt atcgtcaacg
     2041 cttgcgcatg gcccgatttc cctccgtcgc tgcgttcgtt ctgatacgga agcggaatag
     2101 aacacgtgtt ctattctcgc gttcctgtga a
//
LOCUS       DASTMD010000061         6365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2951989, whole genome shotgun sequence.
ACCESSION   DASTMD010000061 DASTMD010000000
VERSION     DASTMD010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2951989"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1275)
                     /locus_tag="VFQ54_00920"
     CDS             complement(61..1275)
                     /locus_tag="VFQ54_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163569.1"
                     /translation="MLRTSFSRRWHGLRATVVLMLSVVGMGATGGDLALADEATPIME
                     TGTLVVTVTMGDGSPVPARGVDVCISAVNGTLSTCQHLADATTTFANLPPDSYYYTIK
                     TSGFDEYSGDVTVYAGETSVLDATLAKSAAAGTAVFELSTVDGGSVPSNANACLHVPG
                     GATYCGHVFETEVLVEHVPVGTYDYLVNPYGGIGAVPANLAQVGGAARGYASLSSRAR
                     LPFAAQTGYVYGSVAGTIEILAGEDSVVNVVLPLQSDLNATLTPQVTPTATEPVLDPG
                     SPEAETSPTMAPTDGVATPPAPNPGTSPVARATTGPISSSVPAPAASPTVVPTGEAAT
                     RPVSGPGSTPIADVSRQQGPGGVSSLPNTGDGSTQRESGFELLLLVGAFTIALLAATV
                     VAGRRRDGRRAD"
     gene            complement(1319..1489)
                     /locus_tag="VFQ54_00925"
     CDS             complement(1319..1489)
                     /locus_tag="VFQ54_00925"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEU0163570.1"
                     /translation="MVYLVRCECGVEIRDADQAELIARVKEHARTQHQLEFTEDQVLA
                     LVEIEVEEEPAS"
     gene            complement(1618..4038)
                     /locus_tag="VFQ54_00930"
     CDS             complement(1618..4038)
                     /locus_tag="VFQ54_00930"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU0163571.1"
                     /translation="MFSAATPVSPSRLFGRAHDLSAIANLLLRDDGVRLLTLVGPGGV
                     GKTRLADVCVREIEGRFLDGALVFHLAELTDSALVIPTMAGALGLQELIGGDPEARLI
                     EFLQPRRMLVLLDNVEHLPGFLPDLARLLDLAPGLTVLATSRMRLGIAGERVFPVLPL
                     DVLANFPQPTFPQIAGSAAVRYFVAQAQRVSPEFALTSANAMTVIGICRQLDGLPLAI
                     EIVAAQRHLATLTGLRDSLPALSQEFAGDPESSDATGRQRTLRDTVRWSYRLLPEEAR
                     RTFRQFAVFAGGGLAPDIATVIGTETAPAQGHLDLLVDANLLVRTDVANGDPRYTMLE
                     TIRDFALAELRIQQEEVAARLRHAERFALRAIEAEQGMVGSALAEATSWYFAELDNLR
                     VAMATAIESDRSEVAGQIVSAMRWFWFITGRWQEAYDWHLRLRGVLTNPDHARIRGWV
                     LIGLTGFARVLGGARDSVTAADESVQIFGDMRDNDGLAMALSTRGFMSIDQGQEQGAL
                     EDLQEAVAMFRTGPPRWELSLALYNLGQGYLRTGRIEDAASSFDEGFTICRRTGSFWG
                     MISNLEVLAEVTIIMGDFARALTLAQESLDLARQFGHKRAITNSAGRLGNVALHFNDI
                     AGARNAFEEALSVAIDIGDPRSVADAIEGVATVAALAGDPQRAAVLLAAATVIRREHA
                     FLAGRHAAEALDALRESLRAELGARRFDAIWERGARLSIFEAVDAAHVVSGAGVLPSE
                     STSTESASMSRLSRREREVLVLIAQGMTDREIAERLFISRPTVSKHVTSILAKLGVPT
                     RAAAAALS"
     gene            4358..5554
                     /gene="moeB"
                     /locus_tag="VFQ54_00935"
     CDS             4358..5554
                     /gene="moeB"
                     /locus_tag="VFQ54_00935"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEU0163572.1"
                     /translation="MPSSRDLLDQVRSQIREIDVPSARTAIADLSPAIIDVREADEVE
                     QGIIPNAVHIPRGFLELRIEDAVPDRSQPVIVYCAGGTRSAFAARTLQDLGYTDVVSL
                     AGGFNAWKGAGERWVQPQTLTGDQQRRYSRHLLIPEIGLAGQIKLLESRVLLIGAGGL
                     GSPIGLYLAAAGVGTLGIIDDDVVDASNLQRQIIHTTDRIGTSKAESARIAIEALNPD
                     VRVEEHRVHLTKENAFDLFRQYDVIVDGSDNFATRYLVSDVCVLLKKPFVHGSIYRFE
                     GHISAFDPRDANSPCYRCLFPSPPPPELAPNCAEAGVLGLLPGTVGLLQATETVKILL
                     GIGETLVGKLLTYNALTTTFRTLRLSKDPHCPMCSDEGPDSIEGITYDEIACAIPHRE
                     AVAVAV"
     gene            complement(6155..>6365)
                     /locus_tag="VFQ54_00940"
     CDS             complement(6155..>6365)
                     /locus_tag="VFQ54_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163573.1"
                     /translation="HPYSPGALSVEGRIPDETIQALIDLGHKVEVWPDWSPRAGSLCT
                     IVVGSDRAPLVAGADPRRLAYAIGW"
BASE COUNT         1156 a         2079 c         2054 g         1076 t
ORIGIN      
        1 actggcatgc atgccagtcc ccattcccgt tttgcgtcgc gatacaccgc gatcgacgga
       61 tcagtccgcc cgtctgccgt cgcggcgccg acccgccacg accgtcgccg ccaggagcgc
      121 aatcgtgaac gcgccgacga ggaggagtaa ctcgaaaccg gactcccgtt gggtggatcc
      181 gtcgccggtg ttcggcagcg aggagacgcc acctggtccc tgctgcctgg atacgtccgc
      241 gatcggcgta ctcccgggtc ccgagaccgg gcgtgtcgcg gcctcaccgg ttgggacgac
      301 ggttggactc gcggccgggg ccggaacgga tgaactgatt ggtcccgtgg ttgctctcgc
      361 taccgggctc gttcccggat tcggtgccgg gggcgtcgcg acaccatccg ttggcgccat
      421 cgtgggactc gtttctgcct ccggtgagcc gggatcgagg acgggttcgg tcgccgtcgg
      481 tgtcacctgc ggcgtgagcg tggcgttcag gtcgctctgc aatggaagca cgacattcac
      541 gaccgaatcc tcgcccgcca ggatctcgat ggtgccggca accgatccgt acacgtagcc
      601 ggtctgcgcc gcgaacggaa gccgcgcccg ggaggagagc gatgcgtagc ctcgcgccgc
      661 acccccgacc tgtgcgaggt tcgccggcac ggcaccgatt ccgccgtagg gattcacgag
      721 atagtcgtat gtgccaacag ggacgtgctc gacgaggacc tcagtctcga acacgtgacc
      781 gcaataggtt gcgccgcccg ggacgtggag gcatgcattg gcattgctcg gcacgctccc
      841 accatcgacg gtggagagct cgaagacggc agtcccggcg gccgcgctct tcgcgagcgt
      901 ggcatccaga acggatgtct cgccagcgta taccgtgaca tcacccgagt attcatcgaa
      961 ccccgacgtc ttgatggtgt agtagtagct atcaggcgga agattcgcaa aggtggttgt
     1021 ggcatcggcg aggtgttgac acgtcgaaag cgttccattc accgcagaga tacagacgtc
     1081 aacgccgcga gccggaacag gactcccgtc tcccatcgtc acggtcacga ccagcgttcc
     1141 cgtttccatg atcggggtcg cctcatcggc cagggcaaga tcgccaccgg tcgcgcccat
     1201 gccgacgacc gaaagcatga gcacgaccgt ggcacgcagg ccatgccatc gccgcgagaa
     1261 actcgtccgc aacatatcgc tcctctcgat tctcttccca cagttatgcg ccaagccgct
     1321 acgatgccgg ctcctcttcg acctcgatct cgacaagcgc aaggacctga tcctcggtga
     1381 attccagctg atgctgagtt cgcgcgtgtt ccttgacgcg ggcaatcaac tccgcctggt
     1441 ccgcatccct gatctcgaca ccacattcac aacgaacgag atagaccatg tttcgccctc
     1501 cgattcgctc atgccgacag gttccgggtc cctgttacga tcgtaggtcg ggtcgcggag
     1561 cgtgggtatg gtgagaactc accatttcct gatggaattg gcggcatctg gccagggtca
     1621 ggagagggcc gcggcggcgg cgcgggtggg gacgccaagc ttggcgagaa tcgaggtgac
     1681 gtgcttggag acggtcggcc gggaaatgaa gagccgctcg gcgatctcgc gatcggtcat
     1741 tccctgcgcg atcaggacga gaacctcgcg ctcgcggcgg ctgagccggg acatcgacgc
     1801 ggattccgtg gatgtggatt cggagggaag aaccccagcg ccagagacga cgtgcgcggc
     1861 atcgacggcc tcgaagatcg acagccgcgc gccacgctcc cagatcgcat cgaaacgccg
     1921 ggcgccgagt tcggcgcgga gcgattcgcg caacgcgtca agggcttcgg cggcatgacg
     1981 acccgcgaga aacgcatgct cgcgacgaat cactgtcgcc gccgcgagca ggacggctgc
     2041 tcgttgcgga tcccctgcca gcgccgcgac cgtcgccacc ccctcgatcg catcggcaac
     2101 cgagcgcggg tccccgatgt cgatggcaac actgagtgcc tcttcgaagg cgttccgggc
     2161 cccggcgatg tcgttgaaat gaagcgccac gttcccgagg cggcccgccg aattcgtgat
     2221 ggcgcgcttg tgaccgaatt gccgtgccag gtcgagactt tcctgcgcca gcgtcagcgc
     2281 tcgcgcaaag tcgcccatga tgatggttac ttcggcgagg acctcgagat tggaaatcat
     2341 cccccagaaa ctccccgtcc ggcggcagat cgtgaatcct tcatcgaacg acgatgcggc
     2401 atcctcgatt cgcccggtgc gcaaataccc ctgtccgaga ttgtagaggg caagcgagag
     2461 ctcccagcgg ggtggtccgg ttcgaaacat ggcaacggcc tcttggagat cctcgagcgc
     2521 accctgctcc tgtccttgat cgatgctcat gaagccgcgg gtggagagcg ccatcgccag
     2581 gccatcgttg tcccgcatgt cgccaaagat ctgaaccgat tcgtccgcgg cggtaacgga
     2641 atcccttgcc cccccaagga cccgcgcgaa tcccgtcaac ccgatcagaa cccatccgcg
     2701 gattcgggcg tgatcgggat tcgtgagtac cccgcgcaga cgcaggtgcc agtcgtaggc
     2761 ttcctgccac cgccccgtga tgaaccagaa ccagcgcatc gcgctcacga tctggccggc
     2821 gacttcgcta cggtcggact cgatggccgt cgccatcgcg acgcggagat tgtcgagttc
     2881 ggcgaagtac caggaggtcg cttccgcgag cgcactgcca accatcccct gctcggcttc
     2941 gatggcacga agcgcgaagc gttccgcgtg gcgcagccgt gcggccactt cttcctgctg
     3001 aattcgcagc tcggcaagag cgaaatcgcg gatcgtctcc agcatcgtgt atcggggatc
     3061 gccgttcgca acgtcggtcc gcacgagcag attcgcatcg acgagaagat cgagatgacc
     3121 ctgcgcgggg gctgtctccg tgccgatcac cgtggcgatg tccggggcga gaccgccacc
     3181 agcgaagacg gcaaactgac ggaaggtacg ccttgcctcc tccggcaaca gccgataact
     3241 ccagcggacg gtgtcccgca gcgttcgttg acggccggtc gcatccgatg attcggggtc
     3301 tcccgcgaac tcctggctga gtgccggcag cgagtcccgc aacccggtga gtgtcgcgag
     3361 atggcgctgc gcagcgacga tctcgattgc gagggggaga ccgtcgagct gacggcagat
     3421 cccaatgacg gtcatcgcat tggccgaggt gagcgcgaac tccgggctga cgcgctgtgc
     3481 ctgcgcgacg aagtagcgga cggccgctga tccggcgatc tgggggaagg ttggttgcgg
     3541 gaaattcgcc agcacatcca ggggcaagac cgggaacacc cgctcgccag cgatcccgag
     3601 tcgcatccgg ctggtcgcaa gcacggtcag tcccggcgcg agatcgagca accgggcgag
     3661 gtccggcagg aagccgggga gatgctccac gttgtcgagc agaaccagca ttcgtcgcgg
     3721 ctggaggaat tcgatcagtc gtgcttcggg gtcgccaccg atcaactcct gcaagccgag
     3781 agcgcccgcc atcgtcggga tcaccagcgc ggaatcggtg agttcggcga gatggaagac
     3841 gagggcgcca tccaggaatc gcccctcgat ctcccgcaca cagacatcgg caaggcgcgt
     3901 cttgccgaca ccgcccggtc cgacgagcgt cagcagacgg acgccatcat cccgcagcag
     3961 gagattcgcg atcgccgaga gatcgtgggc tcgcccgaag agccgtgacg gcgagaccgg
     4021 tgtcgcagcg gaaaacatgc gggcgatcct gactttacca gacttcgttc cgtaaccaga
     4081 cgatccgctc aggcaaggtg cccaccgccg gatcgtccct ggccaatgcc acatgcttcc
     4141 ctgcttgaca tcatagcaat tctgatttcc ataagataca aatctgtgat agctccgccg
     4201 ggatcctcga ggacgggttt gatgccccgt gaccttcccg aatcgcaagc cgggataagc
     4261 cgttcatcga caggcgcggt atcctgagcg tatgagacgg agggccgcac actgtaacgc
     4321 cccctccccc gatcgatttt cctgcggggt tacatccatg ccatcatcac gcgatcttct
     4381 cgaccaggtt cgctcgcaga tccgggagat cgacgtaccg tccgcgcgga cggcgatcgc
     4441 cgacctctcc cccgcgatca tcgatgtccg cgaggcggac gaagtcgagc agggaatcat
     4501 ccccaacgcc gtgcatatcc cgcgtggctt tcttgaactg cggatcgagg acgcggtgcc
     4561 ggaccgatcg caaccggtga tcgtctactg cgcgggcgga acgcgctcgg cgttcgccgc
     4621 ccggacgctg caggacctcg gctacaccga tgtcgtctcg ctcgccggcg gcttcaacgc
     4681 ctggaagggc gcgggcgaac gatgggtgca accgcagacg ctgaccgggg accagcagcg
     4741 acgctacagc cgtcacctgc tgattccgga gatcgggctg gcgggtcaga tcaagctgct
     4801 cgagagccgc gtcctcctga tcggcgcggg tggcctcgga tcgccgatcg ggctctacct
     4861 ggcggcggcg ggcgtcggta cgctcgggat catcgatgac gatgtcgtcg atgcgagcaa
     4921 cctccaacgg cagatcattc acacgacgga tcggatcggg acatcgaagg cggaatcggc
     4981 gcggatcgcg atcgaggcgc tgaatccgga tgtccgggtg gaggagcatc gcgtccacct
     5041 gacgaaggag aacgcattcg acctcttccg ccagtacgac gtgatcgtcg acggctcgga
     5101 caatttcgcg acgcgttacc tcgtgagcga tgtctgcgtg ttgctgaaga agccattcgt
     5161 gcacggcagc atctaccgct tcgagggcca catcagcgcc ttcgatccgc gcgatgcgaa
     5221 cagcccgtgc taccgctgcc tcttcccctc cccgccgccg ccggaactcg cgccgaactg
     5281 tgccgaggcg ggcgtgctcg gcctgttgcc gggcaccgtc ggattgcttc aggcgacgga
     5341 gacggtcaag atcctgctcg gcatcggcga gacgctggtc ggcaagctcc tgacctacaa
     5401 cgcgctgacg acgacattcc ggacgctgcg gctgagcaag gatccgcact gcccgatgtg
     5461 cagcgacgag ggtccggact cgatcgaggg catcacctac gacgagattg cctgcgcgat
     5521 cccgcaccgc gaggccgtgg ccgtcgcggt gtaggccgac gtgcgccgtt tccttcgtag
     5581 cgggtcaccc cgtgtgaccc atcgagcgca gctcgatatg atgccggtgt tgacggcgtc
     5641 taccaatcac cgtcatccgt aatcccgtag atgcggccct tgtggccgcc acgggccggc
     5701 gaagccgacc cgttcgcggg tgatcgtggg tcgcgaggtg cctcgcagta acgttggggt
     5761 tgcggcgggg tatgaaaccc cgccctacaa accccagccg caccgaacct gtcgatagct
     5821 ccggattctg ccgtcgatgc aacgggcggg cgggcgaccg ccggatatcg agcgggggaa
     5881 ccccgcacgt tgcgtcgatc ggcgtaggtg cgcgaggcag gactgcgcaa gcccgcgatc
     5941 ggcattcgcg atgtctcgcg agcagcgggt ccgatcgcgg gctggagtgc tgtcccgcct
     6001 ctggctgacg cgcacgatgc tcccgcgaat atccacccac gaggcaatgg gaggcatcgg
     6061 cggcaaggag ccgtcaatgc ctgcgtcata tcgagctgcg ctcgatgggt cagaccagct
     6121 gacccactac gagactgtgt ctggtcgcat cggcttacca gccgatcgcg taggcgagcc
     6181 ggcgcgggtc ggcgccggcg acgagcggag cgcgatcgga tccgacgacg atcgtgcaga
     6241 gcgatcccgc gcgcggggac cagtccggcc agacctcgac cttgtgacca agatcgatca
     6301 atgcctggat cgtttcatcc ggaattcgcc cctcgacact cagtgcgccg ggcgagtagg
     6361 gatgc
//
LOCUS       DASTMD010000062         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_95117, whole genome shotgun sequence.
ACCESSION   DASTMD010000062 DASTMD010000000
VERSION     DASTMD010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95117"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFQ54_00945"
     CDS             <1..375
                     /locus_tag="VFQ54_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163574.1"
                     /translation="VVVVPRFAGVWLFSLTLGVILGVMLISFVAVPARIIGAKKAQRL
                     PPKEALGRAAAGGALSAVAMAPGFVLDRLGLILLGLHNFHVLGFVLLSIGTALYAAGM
                     SSVKAVKLTVKLGPGTPVIHRE"
     gene            451..1911
                     /locus_tag="VFQ54_00950"
     CDS             451..1911
                     /locus_tag="VFQ54_00950"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEU0163575.1"
                     /translation="MAQKTPLFNTPIPSKIMTPDRVTTRLGVLESFDGMPTDDTAALL
                     LEHLTFLRGVEVFLNTIPAASLEAMRCGLAELGVNAANQVAIFDDLMDSNSLFLTGNT
                     DTVYALSILDLGRDGPTVVEIPAGCGPGTVDDAWFRFVVDMGAPGPDRGQGGKYLLLP
                     PGYDGEVPDGYFTAESPSYVNLIALRGFIVDGSTEPAARMFRDGVKIYPLDLRDDPPP
                     MEFVNGSGQTVNTIHANNIEFYDELARVIDHEPVEIIDPETRGLIASIGIQKGKSFQP
                     DEQLRATLADAAAVGNATARSIFFQTTNPEEFLYEGSSWKRGFIGGNFEFLLDGTGGE
                     RNLDARTAVFYMATLNTPAMVAKMIGRGSQYAWGDRDHAGSYLDGAKTYRLHLPPDAP
                     AKDFWSVVVYDPQTRSELQTGQPLPSKNNAQGSLTVNPDRSVDLYFGPHAPAGFETNW
                     IQTVPGKGWFCLLRLYGPLESWFDKTWRPGEIELSE"
     gene            complement(1928..>2419)
                     /locus_tag="VFQ54_00955"
     CDS             complement(1928..>2419)
                     /locus_tag="VFQ54_00955"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HEU0163576.1"
                     /translation="IGDERTFSQDPKYVLRILSDIGVRALSPGINDPTTATDVLNVVE
                     RLLGYLAARNLGDGLQCDDAGELRVEFPVADWEDFVSVALDEIRLYGIQSAQVSRRLI
                     CVLETLIADVPEKRRPVLLDRLDRTRRAVAALYPDPDDRALALAPDAQGLGGTRPSVG
                     TSD"
BASE COUNT          447 a          790 c          752 g          430 t
ORIGIN      
        1 gtcgtggtag tcccccgctt cgccggcgtc tggctgttca gcctgacgct cggcgtgatc
       61 ttgggtgtca tgttgatcag cttcgtggcg gtgccggcac ggatcatcgg agccaagaag
      121 gcacagaggt taccgccgaa agaagcgctg ggacgcgcgg ctgccggcgg cgcgctcagc
      181 gctgtcgcga tggcaccggg tttcgtgctg gaccgcctcg gcctgattct gctcggcctg
      241 cacaacttcc acgtcctcgg gttcgtgctg ctctcgatcg gaaccgcgct ctacgccgcc
      301 ggaatgtcct cagtcaaagc agtcaagctg accgtcaagc tcggcccggg aaccccagtc
      361 atccaccgtg agtgatctgt gccgccgccg gcagcgtcaa gatctgtgaa ggcgcgatcg
      421 cgccagttcc ccagaccctc ggagtgagtc atggcgcaga agacgccgct gttcaatacg
      481 ccgataccgt cgaagatcat gactccggat cgagtgacta ctcgactggg ggtcttggag
      541 tccttcgacg ggatgccgac cgatgacacc gccgcgctgc tgctggagca tctgacgttc
      601 ctgcgcggcg tcgaagtatt cctgaacacg atcccggccg cgtcgctcga ggcgatgcgc
      661 tgcggtctcg ccgaactggg cgtgaacgcg gctaaccaag tagcgatctt cgacgacctg
      721 atggactcga actcgctgtt cctgaccggg aacaccgaca ccgtgtacgc attgtcgatc
      781 ctcgatctcg gtcgtgacgg gcctacggtt gtcgagattc ccgcgggctg cgggccaggg
      841 accgtggacg acgcgtggtt tcggttcgta gtcgacatgg gcgctcccgg gccagaccgc
      901 ggccagggcg gcaagtatct gctcctgccg cctggctacg acggcgaggt accggacggc
      961 tacttcactg ctgagtcccc gagctatgtc aacctgatcg ctctgcgcgg ctttatcgtg
     1021 gacggcagca ctgaacccgc ggctcgcatg ttccgcgacg gcgtgaagat ctatcccctg
     1081 gaccttcgcg atgacccacc cccgatggag ttcgtcaacg ggtcgggcca gacggtcaac
     1141 acgatccacg ccaacaacat cgagttctac gacgagctgg cgcgcgtcat cgaccacgag
     1201 ccggtcgaga tcatcgatcc cgagacccga gggctgattg ccagtatcgg cattcagaag
     1261 ggcaagtcgt tccaacccga cgagcagctc cgggcgaccc tcgctgacgc tgcggcggtg
     1321 ggcaatgcca ccgcacggtc catattcttc cagacgacga acccggagga gtttctctac
     1381 gagggcagca gttggaagcg cggcttcatc ggcggcaact tcgaattctt gctggacggc
     1441 acgggcggag agcgcaatct cgacgcgcgg actgcggtct tctacatggc tacgctcaac
     1501 acacccgcaa tggtggcgaa gatgatcgga cgtggctccc agtacgcgtg gggcgaccgg
     1561 gaccatgccg gcagctacct cgacggagcg aagacctacc ggcttcatct cccgcccgat
     1621 gcccccgcga aggacttctg gtccgtcgtc gtctacgacc cgcagacccg ttccgaattg
     1681 cagaccgggc aaccgctgcc aagcaagaac aacgcgcaag gcagcctcac agtcaacccc
     1741 gaccgttcgg tcgacctcta cttcgggccg cacgcacccg cgggcttcga gacgaactgg
     1801 atccagacag ttcccggcaa aggctggttc tgtctgctgc gcctctacgg cccactggag
     1861 tcatggttcg ataagacgtg gcgtcccggt gagatcgaac tcagcgaata gcgtcgcgtc
     1921 accactatca gtcggaagtt ccgacgctcg gccgcgtgcc gcccaaacct tgcgcgtccg
     1981 gcgccagagc cagagcacgg tcatccgggt cgggatacag tgcggcgaca gcgcgacgag
     2041 tcctgtccag ccggtcgaga agtacgggtc gccgcttctc cggtacatcc gcgatcagtg
     2101 tttcgagaac acagatcaga cgccgtgaca cctgagccga ctgaatgccg tagagacgaa
     2161 tctcgtcaag ggcgaccgac acgaagtctt cccaatccgc cacgggaaac tcgacccgga
     2221 gctcgccggc gtcgtcgcac tggagcccgt cgccaagatt acgcgcggcc aggtagccga
     2281 gcagtcgttc cacaacattc agcacgtcgg tcgcggtcgt ggggtcgttg atgcccggcg
     2341 agagcgcgcg gactccgatg tcggacagga tccgcagaac gtacttggga tcctgactga
     2401 acgtgcgttc gtctccgat
//
LOCUS       DASTMD010000063         4163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_5540, whole genome shotgun sequence.
ACCESSION   DASTMD010000063 DASTMD010000000
VERSION     DASTMD010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5540"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFQ54_00960"
     CDS             complement(<1..676)
                     /locus_tag="VFQ54_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU0163577.1"
                     /translation="MASSARPTISDAIDPRRTVDFESGVDTICRASSDGIPDAATTVD
                     FAGVVLAMDVDEAPSVTPLTASTFLDLVHPDDVDRMRTVLRELAEDPGRSVRIDFRAW
                     HNGGSWRWMSGDIRRMSLDGTQDVILFDRVDITNDKRAELALASQNRILERVIAGDPV
                     TEILDAICGLIEELDETCVCTILLLDSETQTLSHAASRGSTPEFIEETTEVAVAEGVG
                     SCGTAAY"
     gene            complement(900..1781)
                     /locus_tag="VFQ54_00965"
     CDS             complement(900..1781)
                     /locus_tag="VFQ54_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163578.1"
                     /translation="MADHEGVTTPESMRSPSETGLPVPDVGAGSRDLATESTDPAELR
                     HDLLLAKERLAFYESFDRVIGENIRRSGELMLERIAIREEAEARERANAVAEEARQVA
                     AERERAGRTRLLADLVAEIDRIGESVDSLRTRLANELATTGIDSGPSTEPIDILPQVE
                     TAGETHAERAPEFVPDVPAVETLEPHLPPVAAELEATIEEPEPAGASAPPRTYDVLVH
                     GVSRAAVAVSLQRYLNSLATISDVETREFAEGILRLAITSTREDGLSTEDVSGWSDGG
                     TLSVRHAHPNAIELAIS"
     gene            2328..>4163
                     /locus_tag="VFQ54_00970"
     CDS             2328..>4163
                     /locus_tag="VFQ54_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU0163579.1"
                     /translation="MASRDNAKKQQQVNAESSDQEEISVPVSDETPSPDSVPASTPTR
                     ARRPRAKAVAAETAEPVVASTEDATDAEGEPAPRRRRGVRRRANDRHMALPVVVTDET
                     LLLPHMSIPYPIEDDETALSIDRAMRMDPRLVLILTERRVEGDVIVQEHGTGPSPDLI
                     DLMTDLIQQDLGDEDRIEVQIPDADEDSDVTFEPIQEEHYELCEVGVIAEVGQYISRP
                     GGQDHVILQGIARGVVEEIIQDQPYVAAKVRRVDETVTDPTKTEAAMAAVLEQIESYM
                     SMLPNVPEEVLTMVRSVDEPGWLADLIAFSPEFTSEQRQSLLEALDPIERLRHLSVLI
                     QKRLNVMHLRQEIQSEAQAGMDRQQREYFLREQLRAIQKELGEGSAEEALANEIREKI
                     EAAGMPEEVKTKALAQVERLEQQHPFSPEIGVIRTYLEWLTDLPWAVESEDRLDLEEA
                     ARILDEDHYGLDKVKDRILEFIAVRKLAGDKLRAPILCFVGPPGVGKTSLGKSIARAI
                     GRQYVRMSLGGVRDEAEIRGHRRTYVGAMPGRVIKALRDAKSRNPVMVLDEVDKLGSD
                     TFRGDPSSALLEVLDPEQNTAFSDHYLEVPFDLSKVIFITTANMLD"
BASE COUNT          733 a         1308 c         1389 g          733 t
ORIGIN      
        1 ggtacgcggc tgtgccgcag gagccgacac cctcggcaac cgcgacttcc gtcgtctcct
       61 cgatgaattc gggcgtggag ccacgggatg cggcgtggct gagcgtctgc gtctcgctgt
      121 cgagcaggag gatcgtgcag acgcacgtct cgtccagctc ttcgatcaga ccacagatcg
      181 catcgagtat ctcggtgacg ggatctcctg cgatgacgcg ctcgaggatg cggttctggc
      241 tcgccagggc gagttcggct cgtttgtcgt tcgtgatgtc gacgcggtcg aagaggatga
      301 catcctgtgt cccgtcgagc gacatccgcc ggatgtcgcc actcatccag cgccagctac
      361 ccccgttgtg ccaggcccgg aagtcgatcc tgacactccg tccgggatcc tcggccagtt
      421 cgcgcaacac cgttcgcatg cgatcgacgt catcgggatg cacgagatcg agaaacgttg
      481 atgcggtcag cggtgttacg gacggcgctt cgtcgacgtc catcgcgaga acgaccccgg
      541 cgaaatcgac ggttgtcgcg gcgtccggaa ttccgtcgga ggacgcccgg cagatcgtat
      601 cgacaccgga ctcaaagtca acggtccggc ggggatcgat cgcatcggaa attgtcggtc
      661 gtgcggaaga tgccatacgc ggcggctcca gaagtctgcg agggcggggt ctccggagat
      721 catgttgtat gaagtatcca ctggatggcc tccagtacgc tcgttccaac cggctcgcgg
      781 accgctttcg accgctgatt gacgtagtga aatgggaata aatgcccatt ccagtgaaaa
      841 ggcggccaga aacgccctga atcacgtatc ccgggaaagg aatcccgtgg atggagtcgc
      901 tacgaaatgg ctagttcgat cgcgttggga tgagcgtgtc gaacgctcag cgtgcctccg
      961 tcgctccatc cggagacgtc ctctgtcgac agcccgtctt cccgcgtgga tgtgattgcg
     1021 aggcgaagga tgccttccgc gaactcccgt gtctcgacgt cggagatcgt cgccagggag
     1081 ttgaggtagc gttgcaggga gacggcgacg gcagcccgcg atacgccgtg gaccaggacg
     1141 tcataggttc ggggcggggc ggaggcgcct gccggctccg gttcctctat tgtcgcctcg
     1201 agttcggcgg cgacgggcgg gaggtgtggc tcgagggtct cgacggctgg cacatccggg
     1261 acgaactccg gcgcgcgctc ggcatgagtc tccccggcgg tttccacctg gggcaggatg
     1321 tcgatcggtt ccgttgacgg accggaatca attccggttg tcgccagttc gttcgcgagc
     1381 cgggtccgaa gcgagtccac gctctcgccg atgcgatcga tctcggcgac gaggtcggcg
     1441 agcagccgcg tcctcccggc ccgctcccgt tcggcggcga cctgcctggc ttcctccgcc
     1501 accgcgttcg cgcgttcccg cgcttctgct tcctcgcgga tcgcgatccg ctcgagcatc
     1561 aactcgccgg aacggcggat gttctcgccg atgacccgat cgaacgactc gtagaaggcg
     1621 agccgctcct tcgcgagcag gagatcgtgg cgaagctcgg cggggtcggt cgattcggtg
     1681 gcgaggtcac gcgaaccggc gccgacatcc ggaaccggca atcccgtctc gctgggtgaa
     1741 cgcatggatt cgggggtggt cacgccctcg tggtcggcca ttgcgggatg tcctcgttct
     1801 tgtacgtgcg tgcgtggaat ggctccggaa gcgcgccgga tgtggttgcg agtgtagcaa
     1861 gtcacggggc gcgtgagcgg cgtgggtccc gcggcggtat ggatccgggt gggccgcacc
     1921 ggcgctacga accgctgccc cgggggggtg gccacttggg gtagcggcgc gcggatccgt
     1981 cgtcgcgatg aacgggacat atgggcaggt tgccatgccc gatgccccta tactttgtgt
     2041 ttcgggtaaa ctccgagcat ggtgacgtac atcgcacatt ccacccgtga catcgaaccc
     2101 gtgttgccac ctgacaattc cacccttggc cgatgcgacc cggggatgct ctgatgcgca
     2161 gtcgcgcagt tcacctgcac ggcggcccgc cggggggatt cttccggcgg gaacgactta
     2221 tccaccttcg aatgtgcacc ctacggtgcg ggtcccgatc gacagacgcg atacccgaac
     2281 gcgaatgatc gaagacctgc ctccaggaaa gttgagacga tttcaacatg gcttctcgcg
     2341 ataacgcgaa aaagcagcag caagtaaacg ccgagagctc cgatcaggag gagatctccg
     2401 taccggtgtc cgatgagacg ccgtctcctg attccgtgcc cgcgtcgacc ccgacgcgag
     2461 cgcgtcgccc gcgtgccaaa gcggtcgcgg ccgaaactgc cgagcccgtc gtcgcctcca
     2521 ccgaggatgc gaccgacgcg gagggcgaac ccgcaccgcg acgacgacgt ggagttcgcc
     2581 gtcgcgcgaa cgaccgtcac atggcgctgc cggtcgtcgt caccgacgag accctgctcc
     2641 ttcctcatat gtcgatcccg tatccgatcg aggatgacga gaccgcgctt tcgatcgacc
     2701 gcgcgatgcg gatggatccg cgcctcgtgc tgatcctgac cgagcggcgg gtcgaagggg
     2761 acgtgatcgt ccaggagcac gggactggcc cgtcgccgga cctgatcgac ctgatgaccg
     2821 acctcatcca gcaggatctg ggtgacgagg atcggatcga ggtgcagatc cccgatgctg
     2881 acgaggattc cgacgtcacg ttcgagccga tacaggaaga gcactacgag ctctgcgaag
     2941 tcggtgtgat cgccgaggtc gggcaataca tctcgcggcc gggcggtcag gaccacgtca
     3001 ttctccaggg catcgcccgc ggcgttgtcg aggagatcat ccaggatcag ccgtacgtcg
     3061 ccgccaaggt ccgccgtgtc gacgagaccg tgacggaccc gacgaagacg gaagcggcga
     3121 tggccgccgt gctcgagcag atcgaaagct acatgtcgat gctgccgaac gtgcccgagg
     3181 aagtcctgac gatggtgcgc agcgtcgacg agccgggttg gctcgccgac ctgatcgcct
     3241 tctcgccgga gttcacctcc gagcagcggc agtcgctcct cgaggcgctc gatccgatcg
     3301 agcggctgcg ccatctgagc gtgctcatcc agaagcgcct caacgtgatg catctccgcc
     3361 aggagatcca gtccgaggcg caggcgggga tggatcgcca gcagcgggag tacttcctgc
     3421 gcgagcagct ccgggcgatc cagaaagaac tcggcgaggg atcggccgag gaggccctcg
     3481 cgaacgagat tcgcgagaag atcgaggccg ccgggatgcc ggaggaggtc aagacgaagg
     3541 cgctggccca ggtcgagcgc ctggaacaac agcacccctt ctcgccggag atcggtgtga
     3601 tccgcaccta tctcgaatgg ctgaccgatc ttccctgggc ggtcgaatcc gaggaccggc
     3661 tcgatctcga ggaggctgcg cggatcctcg acgaggatca ctacggtctc gacaaggtga
     3721 aggaccgcat cctcgagttc atcgccgtgc gcaagctcgc cggcgacaag ctgcgggcgc
     3781 cgatcctctg cttcgtcggc cccccgggcg tcggcaagac gagtctcggc aagtcgattg
     3841 cccgcgcgat cggtcgccag tacgtgcgga tgtcgctggg cggcgtgcgt gacgaggccg
     3901 aaatccgcgg tcaccgccgc acctacgtcg gtgcgatgcc gggccgggtg atcaaggcgc
     3961 tccgcgatgc gaagagccgc aacccggtga tggtcctcga tgaggtcgac aagctcggtt
     4021 cggacacgtt ccgcggcgat ccatcctcgg cgctgctcga ggtgctcgac ccggagcaga
     4081 acacggcctt ctccgatcac tatctcgaag tgccgtttga cctctcgaag gtcatcttca
     4141 ttacgacggc gaacatgctc gat
//
LOCUS       DASTMD010000064         3568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1165985, whole genome shotgun sequence.
ACCESSION   DASTMD010000064 DASTMD010000000
VERSION     DASTMD010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165985"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VFQ54_00975"
     CDS             <1..1207
                     /locus_tag="VFQ54_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0163580.1"
                     /translation="LKASDVKYTFERIIDPKTASSWASQLSLVDSIEATDDATVTFKM
                     KSPYGPFLATASASFAAILPQASPEIDFQSTAVGTGPFAWGKYTKDTETVLNANPTYW
                     DAGFPKLASLDYKILPDEATRLASIRTGEVQLTTLTDPVSVDTASKSDGVTTISQDTT
                     DYYLLGLNCAKAPFNNEKVRQALSLAIDRQAIIDAVFFGNGKVSGPIAPTLGDWSLPA
                     DQLPNYKVDTDQAKALLAEAGVSDLSFKIVVGDLTTDFQNIALVIQDQLKKIGVDAQL
                     DQVEWGTFIDRWKAKDFESFVSYNGSGNDPDRALYPTVYSTGSTNVFGYSDPNVDKLL
                     DQGRATADADARKKIYQEIETTLSTASPIIFLNTRVAQYALRDTVQGFKPSAAQTWDT
                     LKETTVSGS"
     gene            1298..2254
                     /locus_tag="VFQ54_00980"
     CDS             1298..2254
                     /locus_tag="VFQ54_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0163581.1"
                     /translation="MFQFLARRIITVFIPTLLGISILVFGAMRLIPGNFVDVLIGIGP
                     DVSPEQRANIAKSYGLDKPLPIQYLNWLGNVLRGNMGHSLRTGDPVSTEILHKLPPTL
                     ELTLLATIISLLIAIPLGVLAALNRGGILDGFARLIGLFGLSVPNFLLATLLILFVST
                     KWQVLPTIGYVSLSHGLWPNIKSMILPSLSLGALLAASILRMTRSAVLEELGREYLTV
                     ARAKGMTNRAVVMAHLLKNALIPIITVVGIQIGYLLGGTVIVENIFAIPGIGRLALDS
                     VNQRDYPLVQGTTLIIAGFFVLMNLITDVLYGLVDPRIRVGE"
     gene            2254..3168
                     /locus_tag="VFQ54_00985"
     CDS             2254..3168
                     /locus_tag="VFQ54_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0163582.1"
                     /translation="MSSASTVTTPLAEGALATTRRRRPGLLQMFGRQNKFGVVAAGIF
                     VILVLGAIFAPLIAPNDPTLMAPIQRLKAPSSAHLLGTDVFGRDVLSRIIYGLRISIG
                     LGVVSVLVAAVIGTLFGLIAGYIGRWQEYLIMRLTDVLFSFPSILLAILIVSIWGAGL
                     KSIVIAIALVQTPIFIRIVRGSVLAIKHAEYIQASVTAGASTTRILLRHLLPNVAAPI
                     LVQLTLSMSGAVVMEAALSFLGLGAVPPQPSLGSMLSENRASMELAPWTVLYPGLALA
                     ILVLSINIVGDGVRDLLDPRLRSATTNN"
BASE COUNT          607 a         1235 c         1051 g          675 t
ORIGIN      
        1 gttgaaggcc agcgacgtca agtacacctt cgagcggatc atcgatccga agaccgcctc
       61 ctcgtgggcc agccagctct cgctcgtcga cagcatcgag gcgaccgacg acgcgacggt
      121 caccttcaag atgaagtcgc catacgggcc gttcctcgcc accgcctcgg ccagcttcgc
      181 cgcgatcctg ccgcaggcat ctcccgagat cgacttccag tcgaccgccg tcgggacagg
      241 tccgttcgcc tggggcaagt acaccaagga caccgagacc gttctcaatg ccaatccgac
      301 gtattgggat gccggtttcc cgaagctcgc ctcgctcgac tacaagatcc tgccggatga
      361 ggccacccgt ctcgcctcga tccgcaccgg cgaggttcag ctcaccacgc tgaccgaccc
      421 ggtctccgtc gataccgcct ccaagagcga tggcgtgacg acaatctcgc aggacaccac
      481 cgattactac cttctcggtc tcaactgcgc gaaggcgccg ttcaacaacg agaaggttcg
      541 ccaggcactc agcctcgcga tcgaccgcca ggcgatcatc gacgccgtct tctttggcaa
      601 cggcaaggtt tccggcccga tcgcgccgac cctcggcgac tggtcgctgc cggccgacca
      661 gctcccgaac tacaaggtcg ataccgacca ggcgaaggcg ctcctcgccg aggccggtgt
      721 cagcgatctc tcgttcaaga ttgtcgtcgg cgacctgacg accgatttcc agaacatcgc
      781 cctcgtcatc caggaccagc tcaagaagat cggcgtcgat gcccagctcg accaggtcga
      841 atggggcacg ttcatcgatc gctggaaggc gaaggatttc gagagcttcg tgagctacaa
      901 cgggtccggc aacgacccgg accgcgcgct ctacccgacg gtctactcca ccggctcgac
      961 caacgtcttc ggctacagtg acccgaatgt cgacaagctg ctcgaccagg gccgcgcgac
     1021 ggcggatgcc gacgcccgca agaagatata ccaggagatc gagacgacgc tttcgaccgc
     1081 gtcgccgatc atcttcctca acacgcgcgt cgcccagtac gcgctgcgcg atacggtcca
     1141 gggcttcaag ccgtccgccg cgcagacgtg ggatacgctc aaggagacga ccgtctccgg
     1201 ctcatgatcc tccgttcatg agcaacatca cgggggctgg gccatcgctc agcccccgtg
     1261 atgtgccggc gccgatcacg aatgaatcca ggggtccatg tttcagtttc tcgcccgccg
     1321 catcatcacg gtcttcatcc cgacgctgct ggggatctcg atcctcgtct tcggggcgat
     1381 gcggctcatt ccgggcaatt tcgttgacgt cctgatcggc atcggcccgg acgtcagccc
     1441 cgagcagcgc gcgaacatcg caaagtccta cggtctcgac aagccgttgc cgatccagta
     1501 cctgaactgg ctcggcaatg tcctccgggg gaacatgggg cattcgctcc gtacgggtga
     1561 tcccgtctcc accgaaatcc tgcacaagct gccgccgacg ctcgaactga ccctgctggc
     1621 gacgatcatc tcgttgctga tcgcaattcc gctcggtgtc ctcgccgcgc tcaatcgtgg
     1681 cggcatcctc gatggcttcg cccgcctcat cggtttgttc gggctctccg ttccgaactt
     1741 cctgctcgcc accctgctca tcctcttcgt ctcgaccaag tggcaggtgc ttcccacgat
     1801 cggctacgtc tcgctctcgc acgggctctg gccgaacatc aaatcgatga tcctcccctc
     1861 cctctcgctg ggagcgctgc tcgccgcatc gatcctgcgg atgacgcgtt ccgccgtcct
     1921 cgaggagctc ggtcgcgaat acctgaccgt cgcccgcgcc aagggaatga cgaatcgcgc
     1981 cgtggtgatg gcgcatctgc tcaagaacgc gctgatcccg atcatcacgg tcgtcgggat
     2041 ccagatcggc tacctgctcg gaggcaccgt catcgtcgag aacatcttcg cgatccccgg
     2101 catcggccgc ctcgcgctcg attccgtcaa ccagcgcgac tatccgctcg tgcagggaac
     2161 gacgctgatc atcgcgggct tcttcgtcct catgaacctg atcaccgatg tcctctacgg
     2221 tctcgtcgat ccgcgcatcc gggtaggtga gtgatgagca gcgcatcgac ggtcacgact
     2281 ccactcgcgg agggagcgct ggcgaccacc cgccgtcggc gcccgggtct cctgcaaatg
     2341 tttggacgcc agaacaagtt cggcgtcgtg gcggcgggaa tcttcgtgat tctcgtgctc
     2401 ggcgcgatct tcgcgccgct gatcgccccg aacgatccca cgctgatggc gccgatccag
     2461 cgactcaagg cgccatcgag cgcccatctc ctcggcaccg atgtcttcgg gcgggacgtg
     2521 ctgagccgga tcatctacgg cttgcggatc tcgatcgggc tcggcgtcgt ctcggtgctg
     2581 gtcgccgccg tgatcgggac gctgttcggc ctgattgcgg ggtacatcgg acgctggcag
     2641 gaatacctga tcatgcgcct gaccgacgtg ctcttctcgt tcccgtcgat cctgctggcg
     2701 atcctgatcg tctcgatctg gggcgcgggt ctgaagagca tcgtgatcgc gatcgccctg
     2761 gtccagaccc cgatcttcat ccgcatcgtg cggggatcgg tgctggcgat caagcacgcg
     2821 gagtacatcc aggcatcggt cacggcaggc gcgtcgacga cgcggatcct gctccggcat
     2881 ctcctgccga acgtcgccgc gccgatcctc gtgcagctga cgctctcgat gtcgggcgcc
     2941 gtggtgatgg aggcggcgtt gagcttcctc ggcctcggcg cggtgccgcc gcagccgtcg
     3001 ctcggctcga tgctgagcga gaatcgcgcg tcgatggaac tcgcgccgtg gacggtgctt
     3061 tatccggggc tcgcgctggc gatcctggtc ctgagcatca acatcgtcgg cgacggcgtc
     3121 cgtgatctcc tcgatccgcg tcttcgttcc gctacgacga acaactgacc gattccggca
     3181 gatccgccgg gaagatggtc gaggcaagca gcgggcgagc acgaggctgg cccccacgga
     3241 atgacgtacg actacggctt tccgtagcgg gtcacctcgt gtgacccatc gggcgcagcc
     3301 cgatatagac gatccattcg cggtgtattg ccgttcgcga tcttacagcg aaaaccgaca
     3361 cggttgcccc ttggggggcg tacttcgccc gagtccgctt tgcggcgggg tatgaaaccc
     3421 cgccctacca accccgtacc agcaaaacgg gtcgacggtt ctggatttcg atgtggtatc
     3481 acacaccggc ggcggcacat ccgccgagcc ggggtgcatc gtccctgcca ggattccgat
     3541 ccgtccggcc cggtggctcc atatccgc
//
LOCUS       DASTMD010000065         1660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_95457, whole genome shotgun sequence.
ACCESSION   DASTMD010000065 DASTMD010000000
VERSION     DASTMD010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95457"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VFQ54_00990"
     CDS             complement(<1..804)
                     /locus_tag="VFQ54_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEU0163583.1"
                     /translation="MAQQRDRVILAIDQGTTSSRAVIVDATGAILGSVQREITQIYPA
                     PGWVNQDANHIWEVTLDVTRRVLAETGIDRARIAGIGITNQRETTVLWDRATGEPGAP
                     AIVWQSRQSSAIVDEIVARGMGETYQRITGLVPDAYFSATKIAWLLARDEDLRRRAEA
                     GEICFGTIDTWLIWKLSAGRVHVTDYGNASRTMLFDIRTREWSDELLRDLAIPRAILP
                     ELRGNSEIIVETDPSVLGWSIPIAGVAGDQQAALFGQACFEPGEAKNTYG"
     gene            925..>1660
                     /locus_tag="VFQ54_00995"
     CDS             925..>1660
                     /locus_tag="VFQ54_00995"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEU0163584.1"
                     /translation="MYELPYNLNEEDDLPTRVMKAVGDRPIALDPLPGALVADVYRAT
                     FADGTEAVVKYDAEPEARLDIEAEMLRNLRTPDVIPVPDVYHASRHLLVQELIPGDHL
                     HPEEHEDAGRLLAGLHAVTSPEAGLGGETLNGSILIPSPWTSSWIEFFREHRLGFATD
                     LARDRSQLPAPYWERIQTLAMRLDQIIEEPDACSLLHGDVWDANVLSRGSRVAAFIDP
                     STCFGHPELELAYMSLFGGFGDADAPI"
BASE COUNT          278 a          555 c          521 g          306 t
ORIGIN      
        1 gccgtatgtg ttcttcgcct cgccgggctc gaaacaggct tgtccgaaga gcgcggcctg
       61 ttggtccccg gcaacaccgg caatcgggat cgaccaaccg agaaccgacg ggtcggtctc
      121 tacaatgatc tccgagtttc cacgaagttc cggcagtatc gcgcgcggaa tcgcgaggtc
      181 gcgcaggagc tcgtccgacc actcgcgggt gcggatgtcg aagagcatcg tccgcgacgc
      241 gttcccatag tccgtgacgt ggacgcgacc agcggagagc ttccagatca gccaggtgtc
      301 gatcgtgccg aagcagatct ctcccgcctc cgcccggcgg cgcaggtcct cgtcccgcgc
      361 caggagccag gcgatcttcg tcgccgagaa gtaggcgtcg ggaacgaggc cagtgattcg
      421 ctggtaggtt tcacccatgc cccgcgcgac gatctcgtcg acgatcgccg aggattggcg
      481 actttgccag acgatcgccg gcgcgccggg ttcaccggtc gcacggtccc agagcacggt
      541 cgtctcgcgc tggttcgtga tcccgatccc ggcgatccgg gcgcgatcga tcccggtctc
      601 ggcgaggacg cgacgcgtca cgtcgagcgt gacctcccag atgtgattgg cgtcctgatt
      661 gacccaccct ggcgcgggat agatctgcgt gatttcccgt tgcacagagc cgagaatcgc
      721 accggtcgca tcgacgataa ccgcgcgact cgacgtcgta ccttgatcga tggcaaggat
      781 gaccctatcg cgttgttgcg ccattgttcc cccgttccgc gtcgtctggg ctgcatttgc
      841 attcatgagg ggcatgctac cgcagcgagg cgctccgatg ccgcgaagtt ggtccgggtg
      901 tctcgaatgt gcgatcctcg gcgaatgtat gaactgccct acaacctcaa cgaagaagac
      961 gatctcccga cccgcgtcat gaaggcagtc ggcgatcgcc cgatcgcgct cgatccgctt
     1021 cccggtgcgc tcgtcgccga cgtctaccgg gcgacgttcg ccgacggcac cgaggcggtc
     1081 gtgaaatacg acgcggagcc cgaggcgcgc ctcgacatcg aagccgagat gttgcggaac
     1141 ctgcgtacgc cggacgtgat ccctgttccc gacgtctatc acgcatcgcg gcatcttctc
     1201 gtccaggaac tgatcccggg cgaccacctg cacccggaag agcacgagga tgccggacgc
     1261 cttctcgcgg gcctgcacgc cgtgacatcg ccggaagccg gtctcggcgg cgagacactg
     1321 aacggatcca tcctgatccc gagtccatgg acgtcgtcgt ggatcgagtt ctttcgcgag
     1381 caccggctcg ggtttgccac cgacctcgcc cgcgaccgaa gccagttgcc ggcgccgtat
     1441 tgggagcgca tccagacgct tgcgatgcgc ctcgaccaga tcatcgagga accggacgcg
     1501 tgctcgctcc tccacggtga tgtgtgggac gcgaatgtac tctcacgcgg cagccgcgtc
     1561 gccgcgttca tcgatccgtc gacctgcttc ggtcatcccg aactggagct cgcctacatg
     1621 tcgctcttcg ggggttttgg cgatgccgac gcgccgatct
//
LOCUS       DASTMD010000066         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1791976, whole genome shotgun sequence.
ACCESSION   DASTMD010000066 DASTMD010000000
VERSION     DASTMD010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791976"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFQ54_01000"
     CDS             <1..250
                     /locus_tag="VFQ54_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0163585.1"
                     /translation="NGVKGLSVRPIEGAFYGFVDGRETGLDDVTLADRLLREAHVALV
                     PGSAFGESGAGHIRLSFATSDDLLAGAIERIGTVLGTR"
     gene            280..729
                     /locus_tag="VFQ54_01005"
     CDS             280..729
                     /locus_tag="VFQ54_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0163586.1"
                     /translation="MSVEARLKELNITLPEPVNPAANYVRWQRTGNLLFISGTGPADG
                     QPKGKVDTEVSVEEAYQVARSVGLQILATAQAALGSLDKITKTVKILGMVNSAPDFGK
                     QPQVMNGCSDLLVEVLGEAGRHTRSAVGFAALPNQIAVEIEATFEVE"
     gene            974..1444
                     /gene="greA"
                     /locus_tag="VFQ54_01010"
     CDS             974..1444
                     /gene="greA"
                     /locus_tag="VFQ54_01010"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU0163587.1"
                     /translation="MDTKKVEVTREGYDRLSGELRELIEVKRPDIIAAVAEARSHGDL
                     RENAAYDAARQDQAMIEKRIAELEGMLRNVAVLEHAGADGSGQVKVGSSVTVDFDGED
                     EKYTIVGAIEAKPTSGMISNESPIGRALIGKKAGDKVVVQTPGGETTFTIKAVD"
     gene            1497..2249
                     /locus_tag="VFQ54_01015"
     CDS             1497..2249
                     /locus_tag="VFQ54_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEU0163588.1"
                     /translation="MRHPLSWLPDIPVEQQVAYVKAWDHFRSAGSVADGRHDTDDWSS
                     RDTSFAICCVRIPPGTLGPAIEEIRAVLAEYPFVRLHPDDFLHIPIQELGFLSDAPRR
                     RDEINFERLEEFSSVARIPIGEYPQFTIRIGGINSFLDAPFLDVQDGGWISRIHRRLL
                     DIAVIPPNTRFPFLPHITLGHYTESAEIGDLGERLEPWRDITLGEFEVNEIEIVTLRT
                     NEAYPPLVDLHGLPLATASHTTPFTSAPNAPH"
BASE COUNT          449 a          775 c          751 g          404 t
ORIGIN      
        1 caacggggtg aagggattga gcgttcgtcc aatcgagggc gcgttctacg gcttcgtcga
       61 cggccgggag accggactcg acgacgtgac acttgccgac aggctcctgc gggaggcgca
      121 cgtcgcgctc gtgccgggat cggccttcgg cgaatccgga gccgggcaca tccgcctctc
      181 gtttgcgacg agcgacgacc ttctcgcggg tgcaatcgaa cgcatcggca cggtgctcgg
      241 taccaggtag atcgtgcgac tgaaaggaat ttcgagaaaa tgagcgtcga agcccgtctc
      301 aaggaactca acatcacact gccggaaccg gtgaatccgg cggcaaacta cgtccgctgg
      361 cagcgcacgg ggaaccttct cttcatcagc ggcaccggac cagccgacgg ccagccgaag
      421 ggaaaggtcg acaccgaggt cagcgtcgag gaggcgtatc aggtggcgcg gagcgttggc
      481 ctgcagatcc tcgcgaccgc gcaggcggcg ctcggctccc tcgacaagat cacgaagacg
      541 gtcaagatcc tcggaatggt caacagcgcg cccgatttcg ggaagcagcc ccaggtcatg
      601 aatggctgct ccgacctcct cgtcgaggtc cttggcgagg cgggtcgcca cacgcgatcc
      661 gcggttggct tcgccgcgtt gccgaaccag atcgccgtcg agattgaagc taccttcgaa
      721 gtggagtaag gtcaatcatt catcgtcgaa gacacttctg tcgacgatgt cgcggcgtca
      781 tcgcgtggtt caggtgacga acgcgacgcg ggaaggctcc cggcatggca tcattgccac
      841 tgcgggagtc ttcgtccata tcaagcgatg cgcctatcga gcgaccgtcc cgacgcagtg
      901 ggcgacgggc ggcgcgtgag gtcccgcggg tggacgtccc tgatccggtg atgccttgtt
      961 cggaggagta cgaatggata ccaagaaagt cgaagtcacc cgtgaagggt acgatcgcct
     1021 ctccggggag ttgcgcgaac tcatcgaggt caagcggccg gatatcatcg ccgccgtcgc
     1081 cgaagcccgc tcccacggcg acctccgcga gaatgccgcc tatgatgccg ctcgccagga
     1141 ccaggcgatg atcgagaagc ggatcgccga gctcgaaggg atgctgcgaa acgtcgccgt
     1201 cctcgagcac gcgggcgccg atggaagcgg tcaggtcaag gtcggcagca gcgttacggt
     1261 cgatttcgac ggcgaggatg agaagtacac gatcgtcggc gccatcgagg caaagccgac
     1321 gtccggaatg atctcgaacg agtcgccaat cggtcgcgcc ctgatcggca agaaggccgg
     1381 cgacaaggtc gtcgtccaga cgcccggcgg cgagacgacc ttcaccatca aggccgttga
     1441 ctaacctcgt ccagcgggcc gcccaggctc cgatcttcag gggatggcgc cgtcgattgc
     1501 gccatcccct ttcgtggctg cccgacattc ccgtcgaaca gcaagtcgcg tacgtgaaag
     1561 cctgggatca tttccgctcc gccggcagtg tcgccgacgg tcgccacgac accgatgact
     1621 ggtcgtcgcg cgacacgtcg ttcgcgatct gctgcgtgcg aattccaccc ggcacgctcg
     1681 gtcccgcgat cgaggagatc cgcgcggttc tcgcggaata tccgttcgtt cgccttcatc
     1741 ccgacgactt cctgcacatc ccgattcagg agcttggatt cctcagcgac gcgccccgcc
     1801 gccgcgacga gatcaacttc gagcgactcg aggagttctc gagcgtggcg aggattccga
     1861 tcggtgagta tccccaattc acgatccgca tcggcggcat caattcgttc ctcgatgcgc
     1921 cgttcctcga cgtgcaggat ggcggctgga tctcgcggat ccatcgacgc ctgctcgaca
     1981 tcgccgtgat cccgcccaac acgcggtttc ccttcctgcc gcacatcaca ctggggcatt
     2041 acacggaatc ggcggagatc ggcgatctcg gcgagcggct cgagccgtgg cgcgacatca
     2101 ccctcggcga attcgaggtg aacgagatcg agatcgtgac gctgcggacg aacgaggcgt
     2161 atccgccact ggtcgatctt cacgggctgc cgctcgcgac tgcctcccac acgacgccgt
     2221 tcaccagcgc cccgaacgcc ccccactaga ccgctttgcg gcggggcaca gctcgcaccg
     2281 ctctcatccc ggggcgacta gagcaagggt cggaagatac gcctaccgct cccctcctgg
     2341 tcgtcatcct gagcttgtcg aaggatcccc gcgcgaagc
//
LOCUS       DASTMD010000067         1832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2148101, whole genome shotgun sequence.
ACCESSION   DASTMD010000067 DASTMD010000000
VERSION     DASTMD010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148101"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..1789
                     /locus_tag="VFQ54_01020"
     CDS             485..1789
                     /locus_tag="VFQ54_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU0163589.1"
                     /translation="MRIRVKHDGLSVHAIVGSEVVLFGLDVPEEMASRLLGFGFERTD
                     ATSGRCGPLTGLKTFAATEAPGHPQGTPVSTLEHPVQAFLWGDYAVDPGHPYTYRVVA
                     LGGTPENLQSLAETEVSITTESPGSGTHGIFFNRGAAASQSYAREFQNRPPDKVPDRR
                     AWKWLSRGLEEALLGFIADARRGDQLRAAMYEFQYPAVLDAFHAASGRGVDVRIVMDA
                     KANEKPDPGKEAAHGDPRDANLAAIAKAKLGAVTTRREANRSYIAHNKFIVLIRGGKP
                     VSVWTGSTNVTDGGIFGHSNVGHIVRDADVAQAFLDYWTQLEADPTRAKLTPWTEQLA
                     MPEGKPSSGVTTIFSPRKSIAALEWYASLMEGASTSVFFTAALAFPRSCKPSSPRMSI
                     SCGMGSSTTVKTRSSCSNATGTTSSWSGRRSRKRSVDGRPNG"
BASE COUNT          347 a          566 c          591 g          328 t
ORIGIN      
        1 ctgcggtccg gcagcggcgg tcccgctcct tcggctgcgc tcaggacagg ctcccgaacg
       61 accggacgcc gtgcggagag ctgtggcccg cgaattcttg tgggatcgcc cgcagagctt
      121 ggcggagaca agacgaatgt atgagcgaat ggatcgctga atctggcgat ccattcctgc
      181 gtcacatggt ctcggcgcag cacccgtggg tcagccgaag agcctatagc ctccggagtc
      241 gcggaagcta cgatggagga ggaagcacag cgcttcctcc tctttgcagc gacgaccatg
      301 cgtcgtccca acgcggctga tccgcgattc cgccaccgtt tacgctcgcg ccggttcaag
      361 ctgatcagtc gtcgacacga tcgatccccc cgaatccacc acattcgttc ctcgtctcga
      421 cgatagcccg cgccgtgggt gacgccagga caccggcgtt ctcgctgcgg atcggaggtg
      481 tgacatgcgc attcgcgtca aacacgatgg tctgtccgtc catgcaatcg taggcagtga
      541 ggtcgtgctc ttcgggctcg atgtgccgga ggagatggca tcgcgactcc tcggcttcgg
      601 cttcgaacga accgacgcta ccagtggccg gtgcgggccc ctcaccgggc tgaaaacgtt
      661 cgccgccacc gaggctcccg gacaccccca gggcacgcca gtcagcacac tcgagcatcc
      721 ggttcaggcg ttcctgtggg gtgattacgc ggtcgacccc ggtcatccgt acacctatcg
      781 tgttgtcgcc cttggcggga cgccggagaa tctccaatcg ctcgccgaga cggaggtttc
      841 gatcacgacg gagtcgcccg ggtcgggaac ccacggcatc ttcttcaatc ggggagcggc
      901 ggcctcgcag tcgtacgcac gcgaattcca gaatcgacca ccggacaagg tgcccgatcg
      961 ccgtgcctgg aaatggctct cacgtggcct cgaggaggcg ctgctcggat tcatcgccga
     1021 tgccaggcgt ggcgaccagt tgcgcgcggc gatgtacgag tttcaatacc ccgccgtgct
     1081 ggacgccttt catgccgcga gcggacgtgg cgtggatgtc cggatcgtga tggacgcgaa
     1141 ggcgaacgag aaacccgatc cgggaaagga agccgcgcat ggcgatcctc gcgacgcgaa
     1201 tctcgcggcg atcgcgaagg cgaagcttgg tgcggtgacg acgcggcgag aggcgaatcg
     1261 ctcctacatc gcccacaaca agttcatcgt tctgattcga ggtggcaagc cagtctcggt
     1321 ctggacgggc tcgacgaacg tgaccgacgg cggcatcttc gggcattcca acgtggggca
     1381 tatcgtgcgt gatgcagacg tcgcgcaggc cttcctcgat tactggacac aactcgaggc
     1441 ggatccgacg cgggcgaagc tgacgccctg gaccgagcaa ctcgccatgc cggaagggaa
     1501 gccatcatcc ggagtgacga cgattttcag tccgcggaaa tcgatcgcgg cgctggagtg
     1561 gtatgcgagc ctgatggagg gggcgtcgac gagcgtcttc ttcaccgccg ccttggcatt
     1621 tccaaggagc tgcaagccgt cttcgccaag aatgtcgatt tcctgcggta tgggctcctc
     1681 gacaacggtg aagacaagat cgtcatgctc aaacgcgacc gggacaacgt cttcgtggtc
     1741 ggggcgaaga tcgaggaagc gatcggtgga tgggcggccg aacggctgac gggtctcaac
     1801 ggtcatgtgc aatacatcca cacgaagttc at
//
LOCUS       DASTMD010000068         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1077138, whole genome shotgun sequence.
ACCESSION   DASTMD010000068 DASTMD010000000
VERSION     DASTMD010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077138"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1667
                     /locus_tag="VFQ54_01025"
     CDS             <1..1667
                     /locus_tag="VFQ54_01025"
                     /inference="COORDINATES: protein motif:HMM:NF026952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tellurite resistance TerB C-terminal
                     domain-containing protein"
                     /protein_id="HEU0163590.1"
                     /translation="AAQRDAVPLALRMGLGRIAAARKPVPPGWALAWAWYQPGVAPRV
                     GATRRPEVFARLFAHRYHAQYGDGLIVKPGKQKLSLHYRPASQAIADGTLRSNTIPDV
                     FSLAHPVADLVALFTLATMDMESYGRWAGRNPEKAESLAAVAQLPDDFPIATFGDVEP
                     LATWLETSLDGDERVFATGDELMRFWPTTGEKLTRAESVSLAQIIGRLGHGIEPDPRF
                     GGVPIARGSAIVVFREEPGAPREARSAYTTATLLVRLAAAVGTSDGNLSVHELEHLRS
                     VIVSSLQLTPAEQIRLDAHLAWLTTSEIRLTGLKTKLEVLTPQQRTSIGDLLVAVAAS
                     DGAVSPAEVTTLMKLYKMLDLDPESVASHLHARLTSSPPRPATRPVTVRPAGIPEPGF
                     PIPPPPSSTSAPQLVVSPKTSAFTLDEAAIRQKMADTAAVSALLGSIFADDETDVLQS
                     PGGALGERPAIPVTPGIAPDGRPPASVDLVAGLDAVHSAVLRTLATKPAWEAEAFASL
                     ASSHDLMPNGALDVLNESAFEIAGEPLLEGDDPLTVNAYALEAMLA"
     gene            1715..>2999
                     /locus_tag="VFQ54_01030"
     CDS             1715..>2999
                     /locus_tag="VFQ54_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HEU0163591.1"
                     /translation="MPRYRIPKPITATRGPRQPIERERPHWIRFGQRIQIHGITLRDG
                     MIWVGSPDAALGDVAEPSLIDPELPVTPSPGRIPEEIATGPSLSYQNLDPSDRWSYLK
                     WLANGRTGEDAPLAFLLLFLYGIERRLLHAVETDNPSALNELPALGIATFALWERFGN
                     DIDPDLRQHLMNLVDVFDLLSLGVDIYIEDARADIREIVNGPGREITPAPLHVQLGLG
                     GMVAEGLPLPADWAFAWAWYRPEVDDHPDSGNGLDEFIDLFAIRYREAHGDGMMLYPG
                     TQYVRPRIRTCNPTIGIIEIEVGEIPEVISRTTPGAKLVELYNTALEEIAPFRRWLAE
                     SPDATREEVMEHLPERLRTDVEASQAALRTWVEDRLAGKDIAIVDYENIMAQAGNGAR
                     EDPLIAGQSIGMLLAEIGIGVEPDFRNPIELFDPVT"
BASE COUNT          545 a         1039 c          911 g          504 t
ORIGIN      
        1 tcgccgccca acgcgacgca gtgcccctcg cgctccggat gggactcggt cggatcgccg
       61 ccgcgcggaa gccggttcca ccgggatggg cgcttgcctg ggcctggtac cagccaggag
      121 tcgcgccccg ggtaggtgcc acgcgccgac ccgaggtgtt cgcccgtctc ttcgcgcatc
      181 gctatcacgc acaatacggc gacgggctca tcgtgaagcc cggaaagcag aagctctccc
      241 ttcattacag acctgcgagc caggcgattg ccgacggcac gcttcgctcg aacacgatcc
      301 cggatgtctt ctccctcgcc caccctgtcg ccgacctcgt cgcgctcttc accctcgcga
      361 caatggatat ggaatcctat ggccgctggg ccggtcgcaa tccggagaag gccgagagcc
      421 ttgccgcggt cgcccaactg cccgacgatt tcccgatcgc gaccttcggc gacgtcgaac
      481 cactggcgac gtggctggag acctcgctcg acggcgacga gcgggtgttc gctaccggcg
      541 acgagctgat gcgtttctgg ccgacaacag gcgagaaact tacgcgggcc gaaagcgttt
      601 cgctcgccca gatcattgga cgcctcggcc atggaatcga accggatcct cgcttcggcg
      661 gggttccgat tgcccgtggc agcgcgatcg tcgtcttccg tgaggaaccc ggcgcaccgc
      721 gtgaagcgag atccgcctac acgaccgcga cactgctcgt ccggcttgcc gcggcggtgg
      781 ggacatcgga cgggaatctc tccgtgcacg aactcgaaca tctgcgatcg gtgatcgtct
      841 catccctcca gctgacgccc gccgagcaga tccggctcga tgcccatctc gcctggctga
      901 cgacctccga gatcaggctc accgggctca agacgaagct cgaggtgctg actccccagc
      961 agcggacgtc gattggcgat ctcctcgtcg cggtggctgc ctccgatggc gcggtctcac
     1021 cggcggaggt gacgacactg atgaagctct acaagatgct cgacctcgat cccgagtcgg
     1081 tcgccagtca cctccatgcc cggctcacct catcgccgcc gcgaccggca acgagaccgg
     1141 tgacggtacg accggcaggg ataccggagc cggggttccc gatcccgcca ccgccgtcat
     1201 ccacgagcgc gccgcaactg gtcgtctccc cgaagacttc ggcgtttacg ctcgacgagg
     1261 cggcgatccg gcaaaagatg gcggatacgg cagccgtctc cgcgttgctg ggcagcatct
     1321 tcgccgacga cgaaacagat gttctacaat caccgggtgg tgcgctggga gagcgaccgg
     1381 ccatcccggt cacccccggg atcgcgccgg atggccgtcc gccagcgtcg gtcgatctcg
     1441 tcgccggact ggacgccgtg catagcgccg tcctgcgaac actggcgacg aagccagcct
     1501 gggaggcgga ggcgtttgca tcgctcgcct catcgcatga cctgatgccg aacggggcat
     1561 tggatgtgct gaatgaatcc gcattcgaga tcgccggcga accgctcctc gaaggagacg
     1621 atccactgac ggtcaacgca tacgcgctcg aggcgatgct ggcgtagccc tcctgcccat
     1681 tgccgcttcc accatcgaaa gtcgcaggcg atgcatgcca cgataccgaa tccccaaacc
     1741 gatcaccgcg acccgcggcc cgcgtcagcc gatcgaacgg gagcgaccac actggattcg
     1801 cttcggccag cgcatccaga tccacggaat cacgctccgc gacgggatga tctgggtcgg
     1861 cagccccgac gctgcgctcg gggatgtcgc cgagccgtcg ctcatcgatc ccgaattacc
     1921 cgtcacaccg tcgccgggcc gcatccctga agagatcgca actggcccgt cactttcata
     1981 ccagaacctc gatccctccg accgctggtc gtacctgaag tggctggcga atggacgcac
     2041 cggcgaagac gcccccctcg ccttcctcct cctcttcctc tacgggatcg aacgacgttt
     2101 gctccacgcc gtggagaccg acaatccctc cgcgctcaac gagctccccg cattgggcat
     2161 cgcgacgttc gcgttgtggg agcgcttcgg caacgacatc gaccccgatc tccgtcagca
     2221 tctgatgaat ctcgtcgatg tcttcgacct gctctcgctc ggcgtcgaca tctatatcga
     2281 agatgcccgc gcggacatcc gcgagatcgt caatggtccg gggcgagaaa tcaccccggc
     2341 tcccctccac gtgcagctcg gcctcggcgg catggtggcg gaaggactcc cccttcccgc
     2401 ggattgggcg tttgcctggg catggtatcg gccggaggtc gacgatcatc cggattccgg
     2461 gaatggtctc gacgaattca tcgatctctt tgcgatccga taccgcgagg cccacggcga
     2521 cggcatgatg ctctacccgg ggacgcagta cgtcagaccg cggattcgta cctgcaatcc
     2581 gacgatcggc atcatcgaga tcgaggtcgg cgagatcccc gaagtcatct cgcgaacaac
     2641 gcccggagca aagctcgtcg agctctacaa cacggcgctg gaggaaatcg ccccgttccg
     2701 ccgctggctc gcagaatccc cggacgccac aagggaagag gtgatggagc acctcccgga
     2761 acgattgcga acagacgtcg aagcgtcgca ggcggcactc cggacgtggg tcgaggacag
     2821 gctcgccggc aaggacatcg cgatcgtgga ctatgagaac atcatggcac aggccgggaa
     2881 cggtgcgcgt gaggacccgc tcatcgcagg ccagtcaatc gggatgctcc tcgcggagat
     2941 cgggatcggc gtcgagccgg atttccggaa tccgatcgaa ctcttcgatc cagtcacgt
//
LOCUS       DASTMD010000069         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2505679, whole genome shotgun sequence.
ACCESSION   DASTMD010000069 DASTMD010000000
VERSION     DASTMD010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2505679"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFQ54_01035"
     CDS             complement(<1..603)
                     /locus_tag="VFQ54_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0163592.1"
                     /translation="MKSMDSGEVVNVLAEAAVSRRTVLRGATVGGAIAVSAATGLVVP
                     GLGAASVSAQDDSITLNVRSRGEIVNLDPHLGSGNPDATIRDNIYSKLVYTVPADPTK
                     VLPDLATDWTVSADGLEYHFNLRKDAVWHKDYGPVTSKDVVWTFTRVKDPDLGSSMTS
                     LFSNVTETVADGDYAVTVKLATPDVTFLYGTLADSGVIGNE"
     gene            complement(688..1038)
                     /locus_tag="VFQ54_01040"
     CDS             complement(688..1038)
                     /locus_tag="VFQ54_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163593.1"
                     /translation="MAVYQISLMKIKPGRMQDFIDHQEAVREINERFGLKSRRLIRAG
                     LAGPDVGLVQIISEFEDVVAHATWTHERAKDPEYKEWRKSTPGADPDSPGTLLSTALY
                     SDIVPPHYAPSSES"
     gene            complement(1113..2528)
                     /locus_tag="VFQ54_01045"
     CDS             complement(1113..2528)
                     /locus_tag="VFQ54_01045"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU0163594.1"
                     /translation="MAESDIRYQQQIIGDIWTSDAPYRNLEEFCDVIGSRWAASESEH
                     RGGEFLKARLESYGLQNVRLEPVEFGGWTRGDASLVMTAPVAESFSCIALPYCPTGEI
                     EAELIDAGNGESSDFERLGEAVRGKIVIAAGETNAAGAQAKKLAHRTDKLRFAADAGA
                     IGFIFVNQNPGLLHISGGIGAPGGKPAAIFGVGTSWEHGQKILRLAARGGGAARVRLS
                     VGGSFFPNTSYNVVGEIPGSGKSDELVVVGAHYDGHDISQAALDDGAGTMVALEAARV
                     LAALPAEAIGRTIRVVLFCGEEVGLFGSWAYTADHEDERDRTRFMVNLDGAGRGKGGA
                     ESLTLSGRPELMPYFRSYIADSNYKLALTDDLSSHSDHFPYAIRGIPTASIKSPDDSA
                     ALVGRGWGHTEADTFDKATLRGLQMSAMVTARLLLQVASDDEFPGQLMSREDLQQQLT
                     DLELDVALKRSGRWSLVGGPA"
     gene            complement(2581..>2782)
                     /locus_tag="VFQ54_01050"
     CDS             complement(2581..>2782)
                     /locus_tag="VFQ54_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163595.1"
                     /translation="ADLACLKNAKDTVHVRLNREISSLQASFLANTGSDARLPAARHA
                     GRLAPICPFDLFIRPNATIFDP"
BASE COUNT          519 a          874 c          889 g          500 t
ORIGIN      
        1 ctcgttcccg atcacgccgg aatcggccag cgttccgtag aggaacgtca cgtcaggtgt
       61 ggcgagcttg acggtcaccg cgtagtcgcc gtcggcgacc gtctcggtca cgttcgagaa
      121 gagactggtc atgctcgagc cgagatccgg gtccttgacg cgggtgaagg tccagacgac
      181 gtccttggag gtcacaggtc cgtagtcctt gtgccagacg gcgtccttgc ggagattgaa
      241 gtgatactcc agaccgtccg cggagaccgt ccagtcggta gcgaggtcgg gcaatacctt
      301 cgtcggatct gccgggacgg tatagacgag cttgctgtag atgttgtccc ggatcgtcgc
      361 atccgggttg ccgctgccga gatgcggatc gagattcacg atctcgccgc ggctccggac
      421 attcagcgtg atcgagtcat cctgcgccga gaccgacgcc gcgcccagac cgggcacgac
      481 gagccctgtc gcggcggaga ccgcgatcgc accgcccacg gtcgcgccac gcagcaccgt
      541 acgccgtgag actgccgctt cggccaatac attcaccact tcacctgaat ccatggattt
      601 catcacctgc tcctgatgac atgttgatga cggccggaga aaccggggac ccaagcggcg
      661 ttgcttttcg gggaattgcc aggaagatca ggattccgac gacggcgcgt aatgcggcgg
      721 cacgatgtcg gaatagaggg ccgtggagag caaggttccg ggggaatccg gatcggcgcc
      781 cggcgtggac ttgcgccatt ccttgtattc cgggtccttc gcccgctcgt gggtccaggt
      841 tgcgtgcgcg acgacatcct cgaactcgga gatgatctgg acgagtccga catcgggtcc
      901 cgccaggcct gcccggatca gccgccggct cttcaggccg aatcgctcgt tgatctcgcg
      961 aacggcttcc tggtgatcga tgaaatcctg catgcgtccc ggcttgatct tcatcaagct
     1021 gatctgatac acggccatgt ggcgaaatcc ctccatgcga agagcaaacg aagacgcgac
     1081 aatccgggaa tcggcaacgg aaccgaccga gatcaggccg gaccgccgac caggctccaa
     1141 cgccccgatc gcttcagcgc gacatcgagt tcgagatcgg tcaattgctg ctgcaggtct
     1201 tcccgggaca tcagttggcc ggggaattcg tcgtcggacg cgacctggag cagcagccgg
     1261 gcggtgacca ttgccgacat ctgcaggccg cgcagcgtgg ccttgtcgaa ggtatcggcc
     1321 tcggtgtgac cccaaccgcg gcccacgagc gccgccgaat catccgggct cttgatcgag
     1381 gcggtgggaa tgccgcggat ggcatagggg aagtgatccg agtgggagga gagatcgtcg
     1441 gtcagcgcga gcttgtagtt ggaatccgcg atgtaggagc ggaagtaggg catcaactcg
     1501 ggtcgcccgg aaagcgtgag ggactccgcg ccgcctttgc cccgaccagc gccatcgaga
     1561 ttgaccatga accgggtgcg atcgcgctcg tcctcgtggt cggcggtgta ggcccacgat
     1621 ccgaagagcc cgacttcctc gccgcagaag agcacgacgc ggatcgtgcg gccaatcgcc
     1681 tcggcaggaa gcgcggcgag cacgcgtgct gcctcgagcg cgaccatcgt tcccgcgcca
     1741 tcatcgagcg cggcctgcga gatatcgtgg ccgtcgtagt gggcgccgac gacaaccagt
     1801 tcgtccgatt tcccggatcc cggaatctcg ccgaccacgt tgtacgacgt gttggggaag
     1861 aatgaccctc cgactgagag cctgaccctg gcggccccgc caccgcgggc ggcgagacgg
     1921 aggatcttct gcccgtgctc ccaggaggtc cctaccccga agatcgcggc gggcttccca
     1981 cccggggcgc cgatgccgcc gctgatgtgg agcaggccgg gattctgatt cacgaagatg
     2041 aagccgatcg cgccggcgtc cgccgcgaac cgcagcttgt cggtgcgatg cgcgagcttc
     2101 tttgcctggg ccccggcggc gttcgtctcg ccggcggcga tcacgatctt gccacggacg
     2161 gcttccccga ggcgttcgaa atcgctggac tcgccgttgc cggcatcgat cagttccgcc
     2221 tcgatctccc cggtcggaca gtacgggagc gcgatgcagg agaacgattc cgcaaccggt
     2281 gcggtcatca cgagggaggc atctccccgt gtccagccgc cgaattcgac aggctcgagc
     2341 cggacgttct ggagaccgta ggactcgagc ctcgccttga ggaattcacc tccgcgatgc
     2401 tcgctctcgc tcgccgccca gcgcgagccg atgacgtcgc agaactcctc gagattgcga
     2461 tacggagcat cactcgtcca gatatcgccg atgatctgct gctggtaccg gatatccgat
     2521 tctgccatgg cgtcaccctt ctgatatctg atatctcatg ttcgactcgt gagtactgta
     2581 tcaagggtca aaaatggtcg cattaggacg gatgaacaaa tcaaaaggac aaataggggc
     2641 caatcgccca gcgtgacgag cggcaggaag ccgcgcatcg ctccccgtgt tggcgaggaa
     2701 ggacgcctga agcgacgaaa tctcgcgatt caaccgcacg tgaacagtgt cttttgcgtt
     2761 cttcaggcag gccaaatccg cc
//
LOCUS       DASTMD010000070         3613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_631787, whole genome shotgun sequence.
ACCESSION   DASTMD010000070 DASTMD010000000
VERSION     DASTMD010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631787"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFQ54_01055"
     CDS             complement(<1..450)
                     /locus_tag="VFQ54_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163596.1"
                     /translation="MRFSTEPRQVPVQPTETDRSIHRTLFALYDDTIDTELTLSDLRK
                     ANLPATEISVILREQLIDMERSITNQTLLSRVIAVSALDTVSGWLKGLSTLILADRAA
                     YLVAGPIGVVLSTSRDEARLRFWDDWTGSTASGLDLTSGQLARALRRF"
     gene            complement(447..1646)
                     /locus_tag="VFQ54_01060"
     CDS             complement(447..1646)
                     /locus_tag="VFQ54_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0163597.1"
                     /translation="MTNAWTPTRIMVATLVVVGVLAAVWFFVEIGSIILLLILGIIFG
                     AAIEPLVYRFRRAGLPRAQATLLVYVLLFATIGVVLFFLIPQLVNQVQALDKAIPGIF
                     ENLKQHALSSNSYFLEHTGYQTLVRLEDGYYRLRSSPTIGKNQVLDFVSSAIGVLLSA
                     VSMMIVAFYWTTEKSTIKRLVLSFFPFTKRARAHAIWDEIEYRIGGWTRGELSLMVII
                     GAISGIGYYFLGVQYWLALAIVAGLMEIVPFIGPFIGGGSPVLVALADSPKKAIAVLV
                     FVLIYQQVEQGFLVPRIMKNSVGMSPLTVVLTILVGGVLYGPIGAILAIPVGAAVQVL
                     INNLTRLHDDRISDELRDLELSPLSSSAFMSPLAKPGANEDPPPNGRQGGPRSWSDAV
                     RRTARRQ"
     gene            complement(1835..3259)
                     /locus_tag="VFQ54_01065"
     CDS             complement(1835..3259)
                     /locus_tag="VFQ54_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU0163598.1"
                     /translation="MATSNGASRLVINYRNATDVAVGEHGVTAAEIDSLAGLVASEHE
                     RITREHASGEQRWQNLPDDTALADEIVAFAADARAKYKDFILIGIGGSSLGAIATISA
                     LTHPFRNLLPDDRRDGPRIFILDNPDPEKVAATLDTVDLEHTLVNVISKSGQTAETAA
                     NFLVVRDALIKAVGPEKAREQIVATTDEKSGLLRQLSDQEGYRTFPVPDGVDGRQTVL
                     SAVGLIPAALAGIDIHQLLAGAAAMRARTQTGDIRENPAYLLAALSVLADQKHDKAML
                     VTMPYADALYGISDWFRQLWAESLGKKLSVTGEEVFAGQTPIKALGAIDQHSQIQLYT
                     EGPNDKLIQLISIGNYRESVAIPTPPESVSELSYLDGGELGQLLDRERLATSWALTNA
                     GRPNLTITTPTIDAFAIGEFFYLLQLQTVMAGAIYGVNPFGQPGVEAGKNATYALMGR
                     HGYEDLKTELLAEPADAGAYLNRP"
BASE COUNT          649 a         1078 c         1247 g          639 t
ORIGIN      
        1 gaatcgccgc agcgcccggg cgagctgccc gctcgtcagg tcgaggccgc tggccgtcga
       61 accggtccag tcgtcccaga atcgaaggcg cgcttcgtcg cggctcgtcg acaggacgac
      121 gccgatcgga cccgccacga gataggccgc gcggtcggcg agaatcagtg tggagagccc
      181 cttcagccag ccgctgacgg tatccagcgc gctcacggcg atcacccggg acagcagtgt
      241 ctggttggtg atcgagcgct ccatgtcgat cagctgttcg cggaggatga cggagatctc
      301 cgtggcgggc agattcgcct tccggagatc gctcagggtc aactccgtgt cgatggtgtc
      361 gtcatagagg gcgaagaggg tgcggtggat cgaccggtcg gtctccgtgg gctggaccgg
      421 gacctggcgt ggctccgtcg agaatctcat tgccgcctcg cagtccggcg cacggcgtcc
      481 gaccaggaac gcgggccacc ctgtctgcca ttcgggggcg ggtcctcatt cgcgcccggt
      541 ttcgccagcg gcgacatgaa cgcggacgaa ctgagcggcg agagttcgag atcgcgcagc
      601 tcgtcgctga tccgatcgtc gtggaggcgg gtcaggttgt tgatcaatac ctggaccgcg
      661 gcgccgactg ggatcgcgag gatcgccccg atcggtccgt agagcacacc ccccacgaga
      721 atcgtgagca cgacggtcaa cgggctcatc ccaaccgagt tcttcatgat cctcggaacg
      781 aggaatccct gctccacctg ctgatagatc aacacgaaga cgagaaccgc gatcgccttc
      841 ttcggcgagt cggcgagtgc cacgaggact ggcgaaccgc cgccgatgaa cggaccgatg
      901 aacggcacga tctccatcag gccggcgacg atcgcgaggg caagccagta ctgaacgccg
      961 aggaagtagt agccgatgcc cgagatcgcg ccgatgatca ccatcagact cagctcgccg
     1021 cgcgtccagc cgccgatccg gtattcgatc tcgtcccaga tcgcgtgggc gcgagcgcgc
     1081 ttcgtgaacg ggaagaacga gaggacgaga cgcttgatgg tcgacttctc ggtcgtccag
     1141 tagaacgcga cgatcatcat cgagacggcg gaaaggagga caccgatcgc ggaggagacg
     1201 aagtcgagga cctggttctt gccgatcgtc gggctggagc ggagccggta gtagccatcc
     1261 tcgagccgga cgagtgtctg gtatcccgtg tgctcgagga aatagctgtt gctcgagagc
     1321 gcatgctgct tgaggttctc gaagattccc gggatcgcct tgtcgagcgc ctggacctgg
     1381 ttcaccagct gcgggatcag gaagaagagg acgacgccga tcgtggcgaa gagcaggacg
     1441 tagacgagga gcgtcgcctg ggcacgtgga agcccggcgc ggcggaagcg gtagacgagg
     1501 ggctcgatcg cggcgccgaa gatgatgccg aggatgagca ggaggatgat cgagccgatt
     1561 tcgacgaaga accagacggc cgcgagcacg ccaacgacaa ccagggttgc gaccatgatc
     1621 cgggttggcg tccaggcatt cgtcatcgat cgatcgatgc ggtcaggctc gtttggaagc
     1681 gtctcgatac cctccgcggg aacagaataa ccggggcagg cgtgcttccg gggtgatgtg
     1741 acgggtatcg ggaatcagac gacggacgag gtctgattcc cgatgtggct gcagatgttc
     1801 cgaggcaagc gccgtgccgc cgcgcgaccg ctcgctacgg gcgattgaga taggcgccgg
     1861 cgtcggcggg ttcggccagg agctccgtct tgagatcctc gtagccatgg cgacccatca
     1921 gcgcgtaggt ggcgttcttc ccggcctcga cgcccggctg accgaacgga ttgacgccgt
     1981 agatcgcgcc cgccatcacg gtctgcagtt gcaggaggta gaagaattca ccgatcgcga
     2041 acgcatcgat cgtgggagtc gtgatcgtca ggttcgggcg gccggcgttc gtgagtgccc
     2101 aggacgtcgc gagtcgttcg cgatcgagca gctgcccgag ctcgccgccg tcgaggtagg
     2161 agagttcgga gaccgattcc ggcggcgtcg ggatcgcgac actctcgcga tagttgccga
     2221 tcgagatcag ctggatcagc ttgtcgttcg gaccttcggt gtagagctgg atctgggagt
     2281 gctggtcgat cgcgccgagt gccttgatcg gcgtctgccc cgcgaagacc tcttcgccgg
     2341 tgacgctcag cttcttgccg aggctctcgg cccacagctg gcggaaccag tccgagatgc
     2401 cgtagagcgc gtcggcgtat ggcatggtga cgagcatcgc cttgtcgtgc ttctgatcag
     2461 cgaggaccga tagcgcggcg aggagatagg cgggattctc gcggatatcg ccggtctgcg
     2521 tgcgggcgcg catcgcggcg gcgccggcga ggagctggtg gatgtcgatt ccggcgaggg
     2581 ccgccgggat caggccgacg gcgctgagca ccgtctgccg gccatcgacg ccgtcaggaa
     2641 ccgggaaggt ccgataacct tcctgatcgg aaagctggcg gaggagtccg ctcttctcat
     2701 cggtcgtggc gacgatctgc tcgcgcgcct tctcgggtcc cacggccttg atcagggcat
     2761 cgcgaacgac gaggaagttg gcggcggtct ccgcggtctg gccggacttg ctgatcacgt
     2821 tgacgagcgt gtgctcgaga tcgacggtgt ccagcgtcgc ggcaaccttc tcgggatccg
     2881 ggttgtcgag gatgaagata cgcgggccgt cgcgacggtc gtcggggagc aggttccgga
     2941 acggatgggt cagcgccgag atcgtcgcga tcgcgccgag cgacgaaccg ccgatgccga
     3001 tcaggatgaa gtccttgtac ttcgcacggg cgtcagccgc gaaggcgacg atttcatccg
     3061 cgagcgcggt gtcgtcgggg aggttctgcc accgttgctc gccggacgca tgctcgcgcg
     3121 tgatgcgctc atgctccgag gcgacgagcc ccgcgaggct gtcgatctcg gcggcggtca
     3181 cgccatgctc acccaccgcg acgtccgttg cattgcggta attgatgaca aggcgggagg
     3241 ccccgtttga agttgccacg acacatcctt tcattttggg ttcacggttg ctgaatgtgt
     3301 gacttcatgg cacgaatcgg cccgtcacac ctgcctttta cctaggtgat ggtatcactt
     3361 tcacccaagc gtcctgggac accgccacaa ggcttcccgt ggcagattgt ccctgttccg
     3421 gacggcgcga attgacgtcg tgaccgggat gcgttgatat actccccgga gaccgatccc
     3481 gccgaaagac ccgtcgcggt gacgggaggc tggtccgcac ctcgactcgt tcgatcatgc
     3541 caggagcatt cacgatggcc actgtggaga cactcaatcc gagtaccgtt gcgcaggaag
     3601 cgcgcgatca tgt
//
LOCUS       DASTMD010000071         1625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2773940, whole genome shotgun sequence.
ACCESSION   DASTMD010000071 DASTMD010000000
VERSION     DASTMD010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2773940"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VFQ54_01070"
     CDS             <1..644
                     /locus_tag="VFQ54_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163599.1"
                     /translation="LKLATRSGWRSLPPLTHLSWMLMSTPITALATFVAVYPYLWQDP
                     IGRIRTLINFRESEMANQSVISPQFNVTGTLDAFQRTWNNLHARWSGTRSFLTWIGLP
                     DAGRWFESFDVLLGLVGLAVLLYLGLRKGIQSPQLIVLILIVTEILTVEFSLKTDFER
                     YYLPIVLGFVIAGGSAFGVAVGAVQRWVMSDNDTGSTFSWPLIGRGRQPADQR"
     gene            656..>1625
                     /locus_tag="VFQ54_01075"
     CDS             656..>1625
                     /locus_tag="VFQ54_01075"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU0163600.1"
                     /translation="MTNPSNRSRGQYHGLTSLGAVVLIAAIAALGLFSVTNGLLSAQP
                     SEINVAGTPAASPGSSPAASPQASPAAATTSKAVTPDSKQSSSPLDDGADKVIQIIRG
                     TTSGDSRVARVPLSQKHVYDPYQLIKSSDETSLEKDARYLQTNGIPSLIYIRKSDADR
                     ADSQQYADSLRDTWDIESAPNADDGLVILVTMGVRSPRNGTVVFSYGKNTFPVNGLDV
                     NRLNQIYNDNMLPSLAKGQIYSGLYVSVRLMSYYAVFTPGAPPALDSQQKDVSSIVKI
                     AAPVGTILTLVPLIAFWFYPRFADVFGERRRKAVRWGWLLVTSAWVG"
BASE COUNT          298 a          562 c          454 g          311 t
ORIGIN      
        1 agctgaagct cgccacgcgt tccggctggc gctcgctgcc accgctgaca catctctcct
       61 ggatgctgat gtcgacgccg attacggccc tggcgacatt cgtcgccgtc tacccgtatc
      121 tctggcagga tccgatcgga cggattcgga cgctgatcaa cttccgcgaa tccgagatgg
      181 cgaaccaatc cgtcatcagc ccccagttca atgtgacggg gacgctcgac gcattccagc
      241 gcacgtggaa caacctgcac gctcggtgga gcggcacgcg atccttcctc acctggatcg
      301 ggcttcccga tgccggacga tggttcgagt cgttcgatgt cctgctcgga ctcgtgggtc
      361 tcgccgtgct gctctacctc ggtttgcgga agggcatcca gagtccccag ctgatcgtcc
      421 tcatcctgat cgtcaccgaa atcctcaccg tcgagttctc gctcaagacc gacttcgagc
      481 gctactatct gccgatcgtt ctcggattcg tcatcgcggg cggaagcgct ttcggcgtcg
      541 ccgtgggagc tgtccagcgc tgggtcatga gtgacaacga cacgggctcg acgttctcct
      601 ggcccctgat cggacgcggt cgccagcctg ccgatcaacg ttgacaggat ctcccgtgac
      661 aaacccatca aaccgatcca ggggccaata tcacgggttg acatcgctgg gagcggtcgt
      721 attgatcgct gccatcgctg cgctcgggtt gttttccgtc accaacggac tcctgtccgc
      781 ccaaccgtcc gaaatcaacg tcgcgggcac ccccgcggca tccccgggtt cctctcccgc
      841 cgcctcccca caggcttcac ctgcggcggc cacgacgtcg aaggccgtca ccccagactc
      901 gaaacaatcc tcgtcgccac tcgatgacgg cgcggacaag gtcatccaga tcatccgcgg
      961 caccacctcc ggggacagtc gtgtcgcacg cgtgccgctc tcgcagaagc acgtctacga
     1021 tccctatcag ctgatcaaga gcagtgacga aaccagcctc gagaaggacg cccggtacct
     1081 ccagacgaac ggcatcccgt cgctcatcta catccggaaa tccgatgccg accgggcgga
     1141 ttcgcagcaa tacgccgact ccctgcggga cacctgggac atcgagtccg cacccaatgc
     1201 ggacgacggg ctcgtcatcc tcgtcacgat gggagtgcga tcgccccgga acggaacggt
     1261 cgtcttctcc tacggcaaga acacgtttcc cgtcaacggc ctcgacgtga atcggctcaa
     1321 ccagatatac aacgacaaca tgctgcccag cctcgcgaag gggcagatct acagcggcct
     1381 ctatgtctcc gtccgcctga tgtcctatta cgccgttttc acgcccgggg caccgcccgc
     1441 actcgattca cagcaaaagg atgtatcctc gatcgtgaag atcgcggcgc cagtcggcac
     1501 gatcctgacg ctcgtgccgc tgatcgcatt ctggttctac ccgaggttcg ccgatgtctt
     1561 cggggagcgc cgtcgcaagg cagtccgatg gggatggctt ctcgtcacgt cggcctgggt
     1621 cgggg
//
LOCUS       DASTMD010000072         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_364023, whole genome shotgun sequence.
ACCESSION   DASTMD010000072 DASTMD010000000
VERSION     DASTMD010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364023"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFQ54_01080"
     CDS             complement(<1..323)
                     /locus_tag="VFQ54_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU0163601.1"
                     /translation="MSESGKRVAVVLAANYEDIEATSPIEALEQAGADVTVIGIQTGA
                     ISGKKGGSIDATETFASVRPEDFDLLLIPGGGSPENLRIHDAAVEFTRTFANTGKPVA
                     AICHGA"
     gene            458..1831
                     /locus_tag="VFQ54_01085"
     CDS             458..1831
                     /locus_tag="VFQ54_01085"
                     /inference="COORDINATES: protein motif:HMM:NF005034.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0163602.1"
                     /translation="MTDRTNLDDLYQYVDDHANDYLAMLQTLLQQPSIAAQGVGMTET
                     ADLVKGLLGDLGFAPTLVDTRGGFPVVYGELKGEKDRTLSFYDHYDVQPADPLDQWES
                     DPWAGEIRDGRIWARGVSDNKGNIAARVAAIDAWQKVRGPLPLNVKFIIEGEEEIGSP
                     HLYNFAEDHPDLCKADACIWEFGGRDIDGRPQIHLGLKGICYVELRAKGARLDWHSSV
                     ATSVPNPAWRLVWALSSLKGPDEKILIPGFYDDVVPPTEEELKALADLPNTEAERLED
                     LGIPEFLGGLTGIELHKRDYFQPTCTISGFLSGYTGAGSKTVLPSTAMAKVDMRLCAS
                     QDPYKIFASLRKYLDDQGFTDIEAELLGPGYPARTSLTAPIARVVSETYRELYGEEPA
                     IYPTSAGSGPWYQLCSQFGIDACTSGVGHGRSQAHAPNENIYVDDFILEIKHIALIME
                     RYAEYES"
     gene            1882..>2238
                     /locus_tag="VFQ54_01090"
     CDS             1882..>2238
                     /locus_tag="VFQ54_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0163603.1"
                     /translation="MSDINGYIDEHKDRFIEDLRALLRQPSISAQNVGIRETAAMVEQ
                     RFRDLGATVRQIELPGGHPVVYAEFEGASDRTLGFYNHYDVQPPEPLAQWESDPWAGE
                     IRDGRIWARGVADNKGN"
BASE COUNT          427 a          707 c          704 g          400 t
ORIGIN      
        1 gcgccatggc agatcgcggc gaccggcttg ccggtattcg cgaaggttcg cgtgaactcc
       61 accgcggcat cgtgaatccg gagattctcg ggtgatccgc cgcccgggat caataagagg
      121 tcgaaatcct ccggccgcac ggaggcgaag gtttccgtcg cgtcgatgct gccgcctttc
      181 ttgccggaga tcgcgccggt ctggattccg ataacggtga cgtcggcgcc cgcttgctcg
      241 agggcctcga tcggggacgt cgcttcaata tcttcgtagt tcgctgcaag gacaacggca
      301 acccgctttc ctgattcgga catttcggtg aaactccttt gaatacacct cgccgcgaca
      361 ggcaggtacg ctcgatgatc gagtgccatg cacgacgcat tgtgcaagat tgaaaccacg
      421 ttcggtcatc gcaagtgccg gacagaggag acgagtgatg acggatcgca cgaacctgga
      481 cgatctctat caatatgtag acgatcacgc gaacgactac ctcgcgatgc tccagacgct
      541 cctgcagcag ccgagcatcg cggcccaggg tgtcgggatg acggagacag ccgatctcgt
      601 gaagggactg ctcggcgatc tcggattcgc gccgacgctc gtcgatactc gcggcgggtt
      661 cccggtcgtc tacggcgagc tgaagggcga gaaggatcgc acgctcagct tctacgatca
      721 ttacgatgtc cagcccgccg atccgctcga ccaatgggag agcgatccct gggccgggga
      781 gattcgtgac ggccggatct gggctcgcgg cgtttccgat aacaagggaa acatcgccgc
      841 tcgtgtcgcc gcgatcgacg cctggcagaa ggtgcgcggc ccgcttcccc tcaacgtgaa
      901 gttcatcatc gagggcgagg aggagatcgg atcgccgcat ctctataact tcgcggaaga
      961 tcaccccgac ctctgcaagg cggacgcctg catctgggag ttcggtggtc gcgacatcga
     1021 cggccgaccg cagatccatc tcgggctcaa gggcatctgc tacgtcgagc tccgggcgaa
     1081 gggcgcgcgg ctcgactggc attcctccgt tgccacctcg gtgccgaatc cggcatggcg
     1141 actcgtctgg gcgctttcct cgctcaaggg gccggacgag aagattctga tcccgggctt
     1201 ctacgacgat gtcgtgccgc cgacggagga ggaattgaag gcacttgccg atctcccgaa
     1261 cacggaggcg gaacggctcg aggacctcgg catccctgaa ttcctcggcg gtctgaccgg
     1321 gatcgagctc cacaagcgcg actacttcca gcccacctgc acgatctccg gcttcctgag
     1381 cggctacacc ggcgcgggca gcaagaccgt tctccccagc accgcgatgg cgaaggtcga
     1441 catgcggctc tgcgcgagtc aggatcccta caagatcttc gcctcgctcc ggaagtacct
     1501 cgacgaccag ggcttcaccg acatcgaagc cgaactgctc ggtcccggct accccgcccg
     1561 cacgagcctg acagcgccga ttgcccgcgt ggtttcggag acatatcgcg aactctacgg
     1621 cgaggagccg gcgatctacc cgacgtcggc gggttccggt ccctggtacc agctctgttc
     1681 gcaattcggc atcgacgcct gtacctccgg tgtcgggcac ggtcgatcgc aggcccatgc
     1741 gccgaacgag aacatctacg tcgacgactt cattctcgag atcaagcaca tcgccctgat
     1801 catggagcgt tacgcggagt acgaatcctg acctcgatcc cgggttggca agcgattcga
     1861 gattcaggac gggagagttc catgagcgat atcaacggtt acatcgacga acacaaggat
     1921 cgattcatcg aggacctgcg ggccctcttg cgccagccga gcatctcggc gcagaacgtg
     1981 gggattcgcg agacggcggc gatggtcgag cagcgcttcc gcgatctcgg cgcgactgtg
     2041 cgccagatcg aactgccggg tggtcatccg gtcgtctacg ccgagttcga gggcgcgagc
     2101 gatcgcacgc tcggcttcta caatcactac gatgtgcagc cgcccgaacc gctcgcgcag
     2161 tgggagagcg acccctgggc gggcgagatt cgcgatggcc ggatctgggc gcggggcgtt
     2221 gccgacaaca aggggaac
//
LOCUS       DASTMD010000073         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2238519, whole genome shotgun sequence.
ACCESSION   DASTMD010000073 DASTMD010000000
VERSION     DASTMD010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2238519"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFQ54_01095"
     CDS             complement(<1..381)
                     /locus_tag="VFQ54_01095"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU0163604.1"
                     /translation="MSLTNKPIWIGPSVISADFLRLGEQLRELEESGADYLHFDVMDG
                     RFVPNISIGLPILEATRRGSALPIDVHLMIVEPEKWVNEFRAAGADRITVHVEATANI
                     HRVVQAIEASGAVPSVALNPGTPAS"
     gene            555..1361
                     /locus_tag="VFQ54_01100"
     CDS             555..1361
                     /locus_tag="VFQ54_01100"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEU0163605.1"
                     /translation="MNPLDKLEQSIERLFEGTAGALFRQSLKPAELGKRLEQAMRDGR
                     QVSVGKTIVPNKFTVHLHPDDYAPFAKWEAGLCQEMESYLLHSARERDLSFIYKPSVS
                     LVADEHAKRRLPRIESSIVETNPPVQRPMTRPVTPRPQPPTPTYADTTPIRSFAAPAA
                     TAPPREIVFRCIAGDDIGLELTFPQGESSIGRSSDRDAVLRSMHASRNHARLRVVGAR
                     AFIADAGSTHGTYVNGARISGETALSPGDTVKMGNHEFTVSPGDDGWGRR"
     gene            1358..1843
                     /locus_tag="VFQ54_01105"
     CDS             1358..1843
                     /locus_tag="VFQ54_01105"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU0163606.1"
                     /translation="MSFDLGHDAISFDWFILALRIAFIGLVYVFLYQIARVTMRELVK
                     VATVPGASIIPASASASPVISMLEVLDPAQSSLRRGERIPLDLYSTIGRRQDNSIVLD
                     DTFVSNRHAEIVYDGGSWWLEDIGSTNGTIVNDVRATSRIPIGPGDIVQFGRVVTRMT
                     Q"
     gene            1915..>2525
                     /locus_tag="VFQ54_01110"
     CDS             1915..>2525
                     /locus_tag="VFQ54_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0163607.1"
                     /translation="MQHVATSHEHHAQIPGSLSLVLPAYNEEGNIEFVVRRAIEYLPT
                     VVDQFEIIVVNDGSRDSTPQIIDRLAAADSRVKPIHQQNKGYGGAVTAGFRASTGDYV
                     MFMDADRQFDIADIRLLAPFVASFDIVAGFRMERSDPIHRRINAEVFNLVVRILFGVH
                     MRDLDCAFKIFHGDMLRSVELTTSGALINAETQAKLRRQGATL"
BASE COUNT          467 a          837 c          745 g          476 t
ORIGIN      
        1 agaagccggc gtccccgggt tcaaagcgac gctgggaaca gcgccgctcg cctcgatcgc
       61 ctgcacgacg cggtggatgt tcgccgtcgc ctcgacatgg accgtgatcc gatcggcgcc
      121 ggccgctcga aactcgttca cccacttctc cggctcgacg atcatgagat gcacgtcgat
      181 cgggagggcc gagccgcgac gtgttgcttc caggatcggc aggccgatcg agatgttggg
      241 aacgaagcgg ccgtccatca cgtcgaaatg caggtagtcg gcgcccgact cctcgagttc
      301 tcgcagttgc tcgccgagcc ggaggaaatc cgccgagatc acggagggac cgatccagat
      361 cggcttgttc gtcagggaca tggacgtgcg gttctcctgg agtctggtcg tcaggtgggg
      421 ttggattcat catcgtgggc tcgacgcagt accgaaatcg gtagccatgc gtttaccaag
      481 gaagatggta taccatcgtc gggacgtcgg agcatgtacc tgcaacaccc cactggatcc
      541 ggagcaattg tcgcgtgaat ccgctcgata aactcgaaca atccattgag cgactgttcg
      601 agggaacggc cggcgctctc ttccgccagt cgctcaagcc cgccgagctc ggcaaacgcc
      661 tcgagcaggc gatgcgtgac ggacgccagg tctcggttgg aaagacgatc gtccccaaca
      721 agttcaccgt ccacctgcat ccggacgact atgcgccatt cgcgaagtgg gaagccggcc
      781 tctgccagga gatggagtcg tatctccttc actcggcgcg agaacgggat ctctcgttca
      841 tctacaagcc atcggtctcg ctcgtcgccg acgaacacgc gaagcggcgc ctgccgcgaa
      901 tcgagtcgtc gatcgtcgag acgaatccgc ccgttcaacg accgatgacc cgccccgtga
      961 cgccacggcc ccagcccccg acgccgacgt atgccgacac gacgccgatt cgctcctttg
     1021 ccgcacccgc tgccacggcg cccccgcgcg agatcgtctt ccggtgtatc gccggtgacg
     1081 atatcggcct cgagttgacg tttccgcagg gtgagtcctc gatcggtcgc tcctccgatc
     1141 gcgatgccgt ccttcgctcg atgcacgcgt cgcgcaatca cgcgcggctc cgtgtcgtcg
     1201 gcgcgcgcgc gttcatcgcg gacgccggga gcacacacgg cacatacgtg aacggcgccc
     1261 ggatctctgg cgagaccgcg ctttcccccg gggacaccgt caagatgggg aaccacgagt
     1321 tcaccgtgag tcccggcgac gacggctggg gtcgtcgatg agcttcgacc tcggccacga
     1381 cgcgatctcg ttcgactggt tcatcctcgc gctccggatc gccttcatcg ggctcgtcta
     1441 cgtcttcctc taccagattg cccgcgtcac gatgcgggaa ctcgtcaagg tcgcaacggt
     1501 cccgggcgcg tcgatcatcc cggcgtccgc ttcggcctcg ccagtgatct caatgctgga
     1561 ggtgctcgat cctgcccagt catcgctccg gcgcggcgag cggattccgc tcgacctgta
     1621 ctcgacgatc ggtcgccggc aggacaactc gatcgttctt gatgacacct ttgtctcgaa
     1681 ccggcacgcc gagattgtct acgacggcgg ctcgtggtgg ctcgaagata tcggcagcac
     1741 caatggcaca atcgtgaatg acgtgcgggc aacgagccgc attccgatcg gaccgggcga
     1801 catcgtacaa ttcggtcgag tggtcacccg aatgacgcaa taacaaaagc attggatgat
     1861 tccgggccga tgcgcccttt ctattcgcct gacgcaagga ctatatcgaa acctatgcag
     1921 cacgtcgcca cgtctcacga acaccacgcc cagataccag gttcgctctc gctcgttctt
     1981 cccgcctaca acgaggaagg gaatatcgag ttcgtcgtcc gccgcgcgat cgagtacctg
     2041 ccgaccgtcg tcgatcagtt cgagatcatc gtcgtcaacg acggcagccg agacagcacc
     2101 ccgcagatca tcgatcgact cgccgccgcg gactcgcgtg tcaagcccat acaccagcag
     2161 aacaaagggt acggtggtgc agtgactgcc ggtttccgcg cctcgactgg cgattatgtc
     2221 atgttcatgg atgccgatcg gcagttcgac atcgccgaca tccgtctcct cgccccattc
     2281 gtcgcaagct tcgacatcgt ggcgggcttc cgcatggagc gctcggatcc gattcaccgc
     2341 cggatcaatg ccgaggtctt caacctcgtc gtgcggattc tcttcggcgt tcacatgcgg
     2401 gatctcgact gcgcattcaa gatctttcac ggtgacatgc tgcgctcggt cgagttgacg
     2461 acctcgggcg cactgatcaa tgccgagacg caggccaagc tccgccggca gggcgcgaca
     2521 ctcga
//
LOCUS       DASTMD010000074         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2060388, whole genome shotgun sequence.
ACCESSION   DASTMD010000074 DASTMD010000000
VERSION     DASTMD010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060388"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..974)
                     /locus_tag="VFQ54_01115"
     CDS             complement(66..974)
                     /locus_tag="VFQ54_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0163608.1"
                     /translation="MATSTLRVPRAGTRSRQKQAKSRAVRTRSTRIRWTIISIVMWAV
                     ALLFLFPFVWMIISSLKRDIDVFRLPIRWIPHPVSWVSYQKIWDGPYPITRYMGNSLI
                     VAVARVVGEVTTSSLAAYGFARMNFRWKNQIFVLYLATMIIPYQMLLVPRFILYREMH
                     IYDTLWALILPGVFSVFGTFLLRQFFLSIPKDLTDAAKLDGASEFQCYRQIVMPLAKP
                     AIASLTILAFVWSWNDYETPLVMLTTESRFTIPLGLTRYLDEGGGFSPSLIMAGAVSS
                     ILPVLLVFLVLQRQFIAALARSGLKG"
     gene            complement(985..1938)
                     /locus_tag="VFQ54_01120"
     CDS             complement(985..1938)
                     /locus_tag="VFQ54_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0163609.1"
                     /translation="MVADGNSVLVTPNTSNSGVTTGKRTRSRGSWLGLAFIAPNLIGF
                     ALFTLLPLIFALAISFAQWDVVSGLDGIKWVGLRNFREVIHDGNFWHSAKITLIYVGF
                     SVPLTTAFGLLIALALNGPVPGRAILRLIFFIPFIVNAVAISATWILIYHPRYSPLNN
                     TLRQLGVDNPPLWLASSDWALPALIIMAVWAGCGYASVIYLAALQDLPADLFEAAELD
                     GAGVLQKFRAITLPLITPTTFFLLVTGFIGASQSFGMINLMTQGGPGTATTVASYYVY
                     QNGFQFYKFGYAAALAWVMFLFVLLLTLVLWYAQRRSVFYE"
     gene            complement(2083..>3078)
                     /locus_tag="VFQ54_01125"
     CDS             complement(2083..>3078)
                     /locus_tag="VFQ54_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163610.1"
                     /translation="GQRIKAGTAADLSDRVKADADINKWVTGTKGIFTSDNKYYSLPT
                     TQEPNFVFVNKDKLDDAGASLPAAGWTISEFRDLSKNLTKDGAFGSFAPPDTARMTLG
                     PDYWYATGGKTSNFDNPAFKASTQLHRDMIDDGTNFPWTDVLAQNLKAYAQTPFLTEQ
                     SILWINSSFSLRFVGDKDQYPHDFVTTFAPVPFPDDVKDPWNGGSINNWILAKQDSKN
                     LDAAWTFIRFWLVQGGEYMLKAGKVPAFPGIDQDTVVNGILGPDAATLYDVEAYKKVI
                     FDPAIKLVTDTITTGSAQISTIVDGLTDRCLIGEISVDDWVSEVKKQADAAITSA"
BASE COUNT          591 a          886 c         1057 g          544 t
ORIGIN      
        1 acccttcgac gagctcaggg caggctccgt tggtggcccg ccacgggtgc tcaatagcac
       61 aatcgctatc ccttcaatcc ggagcgggca agggcggcga tgaattgccg ctgcaggacg
      121 aggaagacga gcagcacggg caggatcgag gagacagcgc cggccatgat cagcgaggga
      181 ctgaatcctc ctccctcgtc caggtaccgc gtcaggccga gcgggatcgt gaagcgcgac
      241 tcggtggtca gcatcacgag cggtgtctcg taatcgttcc agctccagac gaaggcgaga
      301 atcgtcagcg aggcgatcgc gggtttggcg aggggcatca cgatctggcg gtaacactgg
      361 aactcgctcg cgccgtccag cttcgcggcg tctgtcaggt ccttcgggat cgagaggaag
      421 aactgccgca gcaggaacgt cccgaagacg ctgaagacac cgggcaggat cagcgcccag
      481 agcgtgtcgt agatgtgcat ctcccggtag aggatgaagc gcggcacgag cagcatctgg
      541 tacgggatga tcatcgtcgc caggtagagc acgaagatct ggttcttcca gcggaagttc
      601 atccgcgcga agccgtacgc cgcgagcgac gatgtcgtga cctcgccgac gacccgggcg
      661 acggcgacga tcagcgaatt ccccatgtac cgcgtgatcg gatagggacc gtcccagatc
      721 ttctggtagc tcacccagct gaccggatgc ggaatccagc ggatcggcag ccggaagacg
      781 tcgatgtcgc gcttgagcga cgagatgatc atccagacga aggggaagag gaagagcagg
      841 gcgaccgccc acatcacgat gctgatgatc gtccagcgaa tcctcgtcga gcgggtgcgg
      901 acggcccggc tcttcgcctg cttctgccgg gaacgggtac cggccctggg aacgcgaagg
      961 gtggaagtag ccacggtcgg cctcctattc gtagaagacg ctgcgccgct gggcgtacca
     1021 gagcacgagc gtgagaagca gcacgaagag gaacatcacc caggcgagtg ccgccgcgta
     1081 cccgaacttg tagaactgga atccgttctg gtagacgtaa tacgaggcga ccgtggtggc
     1141 ggtgccgggt ccgccctgcg tcatcaggtt gatcatcccg aacgactgcg aggcgccgat
     1201 gaagccggtg acgagaagga agaaggtcgt cggtgtgatc agcggcaggg tgatcgcgcg
     1261 gaacttctgg agcacccccg cgccatcgag ctcggccgcc tcgaagagat ccgccggaag
     1321 atcctggagc gccgcgagat agatcacgga cgcatatccg catcctgccc agaccgccat
     1381 gatgatcagc gccggcaacg cccagtcgga cgaggccagc cagagcggtg gattgtcgac
     1441 tccgagctgg cgcagcgtgt tgttcagcgg gctgtagcga gggtggtaga tcaggatcca
     1501 cgttgccgag atggcaaccg cgttgacgat gaaggggatg aagaagatca gccgcaggat
     1561 cgcccggccc ggcaccggcc cattgagggc aagggcgatc agcagcccga acgccgtcgt
     1621 gagtggaacg ctgaagccga cgtagatcag cgtgatcttc gcgctgtgcc agaaattccc
     1681 atcgtggatg acctcccgga agttccggag gccgacccac ttgatgccgt ccagcccgga
     1741 gacgacatcc cactgggcga aggaaatcgc gagcgcgaag attaacggga gcagcgtgaa
     1801 gagcgcaaac ccgatcaggt tcggtgcgat gaaggcgagt ccgagccagg agccacgcga
     1861 gcgggtgcgt ttgcccgtcg tgacgccact gttcgaggtg ttgggcgtga ccagcacgct
     1921 gttcccgtct gcgaccatag gtcaacctcc gtgcgaatcg agtccctgcg tcatggatga
     1981 atggatcgag atcttcctgt cgcgtgcgtc cccgggtcgg atggcgattc ggcccggggt
     2041 gtcaccgcga cagcgcgcag gggacacgtc gtgtgcctgc gatcaggcgc tggtgatggc
     2101 ggcgtccgcc tgcttcttga cctcgctcac ccagtcgtcg acgctgatct caccgatcag
     2161 gcagcggtcg gtgagcccgt cgacgatggt cgagatctgg gcgctgccgg tcgtgatcgt
     2221 gtccgtgacg agcttgatcg cgggatcgaa gatcaccttc ttgtaggcct cgacgtcgta
     2281 gagggtcgcc gcgtccggac cgaggatgcc gttgacgacg gtgtcctgat cgatcccggg
     2341 gaaggccggg accttgcccg ccttcaacat gtactcgccg ccctggacga gccagaagcg
     2401 aatgaaggtc caggcggcgt cgagattctt cgagtcctgc ttggcgagaa tccagttgtt
     2461 gatgctgcca ccgttccagg gatccttcac atcgtccggg aatggcaccg gcgcgaaggt
     2521 ggtcacgaag tcgtgcggat actgatcctt gtcgccgacg aaacgaagcg agaacgatga
     2581 atttatccag aggatgctct gctcggtcag gaacggtgtc tgggcgtagg ccttgaggtt
     2641 ctgggcgagg acatccgtcc acgggaagtt cgtgccgtcg tcgatcatgt cgcggtggag
     2701 ctgggtggat gccttgaagg cgggattgtc gaagttcgag gtcttgccgc cggtggcata
     2761 ccagtagtcc gggccgaggg tcatgcgcgc ggtatccggt ggtgcgaagg atccgaacgc
     2821 gccgtccttg gtcaggttct tcgagaggtc gcggaactcg ctgatcgtcc agccggcggc
     2881 ggggaggctg gctcccgcat cgtcgagctt gtccttgttg acgaagacga agtttggctc
     2941 ctgcgttgtc ggcaggctgt agtacttgtt gtccgaggtg aagatgccct tggttccggt
     3001 gacccacttg ttgatgtcgg cgtccgcctt gacgcggtcg ctgagatcgg ccgcagtgcc
     3061 ggccttgatg cgctgacc
//
LOCUS       DASTMD010000075         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1345099, whole genome shotgun sequence.
ACCESSION   DASTMD010000075 DASTMD010000000
VERSION     DASTMD010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345099"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1556)
                     /locus_tag="VFQ54_01130"
     CDS             complement(<1..>1556)
                     /locus_tag="VFQ54_01130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0163611.1"
                     /translation="PASEFEVLFVDDGSGDGTADRLRAVAAERGNVRVIEIENSGWAS
                     RPRNVGIAAAVGEYVTFVDHDDSLFPDALRSAYEAGAAIGADFVNPKEVTDRGWSWGW
                     DVWREDRQLTSREQIVPKDVTPLIPHKLYRREFLNEFGICFPEGPRAFWEDFYLNTEV
                     FVHAESIAILASVPFYKWHRDTGSNSSDRIYESVEEYWAKLGDVARFMEEQVPDAAAR
                     RWLLINHYRWWVIDLFGPRMAKQSPEFIETSLPHMRAFLDRWVPENADDDLEAANAAT
                     AWLMRHSDLDHLRALAVFDGSRTARATTTTGAWTPQGFAITAQATWCGVDGEPLLFRR
                     VGERLIRVLPAPLASVLPVDVIDVSDAIAGAWGGFSVRSRRDRTSWRIESEFESSFDE
                     RGDGAISLRVTLRAVIDPSTLVFGRALEDGIWDLGARFGFMGAEAHAAVDYSDGTKPA
                     IRHGYPVIAYSTNRGALAFDIGGTAKQLVTPGRLDPTSVRRSGSELQINLRGLQMTGS
                     GIGIEVRFGS"
BASE COUNT          284 a          528 c          493 g          251 t
ORIGIN      
        1 cggagccgaa ccgcacctcg atgccgattc cggagccggt catctgaaga ccgcgcaggt
       61 tgatttgcag ctcggaaccc gatcgccgca ccgatgtcgg atcgagccgc cccggggtga
      121 ccagctgctt ggcggttccg ccgatatcga atgccagggc gcctcgattc gtcgagtagg
      181 cgatcaccgg atagccatgg cggatcgcag gcttggtgcc gtcgctgtag tccactgccg
      241 cgtgcgcctc tgctcccatg aagccgaatc gggctccgag atcccagatg ccgtcctcca
      301 gagcacgccc gaaaaccagg gtggaggggt cgatcacggc gcgaagcgtg acgcgaagcg
      361 agatcgctcc gtcaccgcgc tcgtcgaagc tcgactcgaa ctcgctctcg atgcgccacg
      421 acgtacggtc ccgccgcgag cgcacactga aaccgcccca cgcaccggct atcgcatcgc
      481 tgacgtcgat gacgtcgacg ggcaggacgg atgcgagcgg agcgggcagg actcggatga
      541 gccgctcgcc gacgcgtcgg aaaagcaggg gttcgccgtc gaccccgcac cacgtcgcct
      601 gcgccgtgat cgcgaacccc tgcggcgtcc acgccccggt agtggtggtc gccctggccg
      661 tgcgggaacc gtcgaatacc gccagcgcgc gcaggtggtc gagatcggaa tgccgcatga
      721 gccacgcggt ggccgcgttc gccgcctcga ggtcgtcatc cgcgttctcc gggacccagc
      781 gatcgaggaa cgcgcgcatg tgcggcagag atgtctcgat gaactccggc gactgtttgg
      841 ccatgcgcgg gccgaagaga tcgatcaccc accatcgata gtggttgatg agcagccacc
      901 ggcgggcggc ggcatccgga acctgctctt ccatgaaccg ggcgacgtca cccagcttcg
      961 cccagtactc ctcgacgctc tcgtagatcc tgtcgctgct gttggaaccg gtatcgcgat
     1021 gccacttgta gaacggcacg gaggccagga tcgcgatgct ctccgcgtgc acgaagacct
     1081 cggtgttgag gtagaaatcc tcccagaagg ctcggggacc ttcgggaaaa caaatgccga
     1141 actcgttgag gaactcacgg cggtagagct tgtgcggaat gagaggcgtc acgtccttcg
     1201 gcacgatctg ctcgcgcgag gtcagctggc gatcctctcg ccacacatcc cacccccatg
     1261 accaaccccg atcggtgacc tctttgggat tgacgaagtc tgcgccgatc gcggcaccag
     1321 cctcgtaggc ggagcgcagg gcatcgggaa acagggagtc gtcgtgatcg acgaaggtga
     1381 cgtactctcc cacggccgcc gcgatgccca cgttacgcgg ccgactcgcc catcccgagt
     1441 tctcgatctc gatgacacgg acgttcccac gttccgccgc gaccgcgcgc aatcgatcgg
     1501 ccgtaccgtc acccgacccg tcatcgacga agagcacctc gaattcgctc gcgggc
//
LOCUS       DASTMD010000076         3558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_810234, whole genome shotgun sequence.
ACCESSION   DASTMD010000076 DASTMD010000000
VERSION     DASTMD010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810234"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFQ54_01135"
     CDS             complement(<1..196)
                     /locus_tag="VFQ54_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163612.1"
                     /translation="MDQIAQAGAARGSTRQFIGSNRTRWITTAILVFGAAVTIFPFIW
                     MLLGSFKTLIEGLRFPPTFLP"
     gene            complement(205..1305)
                     /locus_tag="VFQ54_01140"
     CDS             complement(205..1305)
                     /locus_tag="VFQ54_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0163613.1"
                     /translation="MARTLVPQQSEAATPAIPARFGLGRLRRESRRTSGWGGLLYILP
                     AFAILILFEFWPIVFSIWISLWRWDVRPLNFIGLDNYRQLFGDGMLTRDYNGNLVPGE
                     VLNSLIVTVYYVIGTVPITLAVAFVLAYMLFRGVKGEGILRTLYFLPNVTASVAVTLV
                     FAWIFNAQTGLANALFDWIGLPVQTWLMDPNPALREILDHFGIHALDNVPDWAAGPSQ
                     ALVVIIIFSIWSSLGFAIIVYLSGLTSISRDLSDAARLDGATEWTIMRTVIWPLLSPT
                     TFFLLVVSTINAFQAFNPIYTLTRNSNVGRSEAGGPLDTTLTITVYIYRNFYERANGV
                     GYAAAVSLLLFFILLALTLLQFRVAGRRVHYQ"
     gene            complement(1274..2182)
                     /locus_tag="VFQ54_01145"
     CDS             complement(1274..2182)
                     /locus_tag="VFQ54_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0163614.1"
                     /translation="MKLTFLGTAAAEGFPDPFCACDNCEAARVEGGPSNRMLSAAVIN
                     DDLLIDFGPDIANAAMQFGIRLHTIPYALQTHPHSDHLAGTTLFGRARMCQVEGLAVT
                     QYFCSASSVARMDEVLGLGKRERSFSDPEVQAEFYLDIHTIAPWQEVQVGPYRVQTVV
                     ANHDMGVEAMLFAIEDTERGGRLFYGTDTGSMPADTWPRLAELGWTFDILVMDHTFGY
                     AGRSGGHMNHEQFLEEVAAARANGVIDDETRIYATHIAHHSNPAHGELVKLAADHGYL
                     IGYDGLTIDTAEKGSLSGTNARTAAI"
     gene            3178..>3558
                     /locus_tag="VFQ54_01150"
     CDS             3178..>3558
                     /locus_tag="VFQ54_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163615.1"
                     /translation="MSHTEFPPLRVTSRLARLRTRFAASTGRRRSDRRMHAGGRAIHG
                     VSAIVLVLGLFLPMYAAGNVAASPQSSSPVDPSAQDSNTVIAAPSQVSITGDFGQFDL
                     TSYDGVWKGVFAVQPGQYNYQFVVT"
BASE COUNT          653 a         1081 c         1202 g          622 t
ORIGIN      
        1 gcggcaggaa ggtcggcggg aagcggagcc cctcgatgag tgtcttgaac gagccgagca
       61 acatccagat gaacgggaag atcgtgacgg cggcgccgaa gaccaggatc gcggtcgtga
      121 tccagcgcgt gcggttcgat ccgatgaatt ggcgcgtcga gccgcgagcg gccccggcct
      181 gggcgatctg atccattggc aatcctactg gtagtgaacg cggcgaccgg cgacgcggaa
      241 ctggagcaag gtcagggcga gcaggatgaa gaacagaagg agggagaccg ccgcggcata
      301 cccgacgcca ttcgcgcgtt cgtagaagtt gcgatagatg tagacggtga tcgtcagcgt
      361 cgtgtcgagc gggccgccgg cctcgctgcg tccgacattc gaattccggg tcagcgtata
      421 gatcgggttg aaggcctgga acgcgttgat cgtgctgacg acgagcagga agaaggtcgt
      481 cggcgagagc agcggccaga tcacggttcg catgatcgtc cactcggtag cgccatcgag
      541 tctcgccgcg tcactgaggt cgcgtgagat cgaggtgagg ccggagaggt agacgatgat
      601 cgcgaagccg agcgacgacc agatcgagaa gatgatgatg acgaccagcg cctggctcgg
      661 acccgccgcc cagtcgggaa cgttgtcgag cgcgtgaatc ccgaagtggt cgaggatctc
      721 gcggagcgcc gggttcggat ccatcagcca ggtctggacc gggagaccga tccagtcgaa
      781 gagcgcgttg gcgagacccg tctgcgcgtt gaagatccag gcgaagacga gggtgacggc
      841 aacactcgcc gtcacgttcg gcaggaagta gagcgtgcgc aggatcccct cgcccttcac
      901 gccgcgaaag agcatgtagg cgaggacgaa cgcgactgcc agcgtgattg gcaccgtgcc
      961 gatcacgtag tagacggtga cgatcaggct gttcaggacc tcgccgggaa ccaggtttcc
     1021 gttgtagtcc cgcgtcagca tcccgtcgcc gaagagctgc cggtagttgt cgagcccgat
     1081 gaagttgagc gggcggacat cccagcgcca gaggctgatc cagatgctga agacgatcgg
     1141 ccagaactcg aagaggatca ggatcgcgaa ggcggggagg atatagagca accctcccca
     1201 gccacttgtc cggcggctct cccgtcgcag gcgaccgagc ccgaagcggg ccgggatcgc
     1261 cggggtggct gcctcagatt gctgcggtac gagcgttcgt gccactgagg gatccctttt
     1321 ccgcggtgtc gatcgtcagg ccgtcgtagc cgatgagata gccgtgatct gccgccagct
     1381 tcacgagttc tccgtgcgcg gggttgctgt gatgcgcgat gtgcgtcgcg tagatccggg
     1441 tttcgtcatc gatgacgccg ttggcgcgag cggcggcgac ttcctcgagg aactgctcgt
     1501 ggttcatgtg gccacccgag cgaccggcat agccgaaggt gtggtccatc accaggatgt
     1561 cgaatgtcca gccgagctcg gcgagccgcg gccaggtgtc cgccggcatc gagccggtgt
     1621 ccgttccata gaagaggcgt ccaccgcgtt cggtgtcctc gattgcgaag agcatcgcct
     1681 cgacacccat gtcgtggttg gcgacaacag tctggacgcg gtaggggccg acctggacct
     1741 cctgccacgg cgcgatcgtg tggatgtcga gatagaactc ggcctggacc tcgggatcgc
     1801 tgaacgagcg ctcgcgcttg ccgaggccga ggacctcgtc catgcgggcg acgctggatg
     1861 ccgagcagaa atactgggtg acggcgagtc cctcgacctg gcacatgcgg gcacgaccga
     1921 agagtgtcgt tccggcgaga tggtcgctgt ggggatgcgt ctgcagggcg taggggatgg
     1981 tgtggagacg gatgccgaac tgcatggcgg cgttcgcgat gtccggcccg aagtcgatca
     2041 ggaggtcatc gttgatgacg gcggcggaga gcatgcggtt cgacggcccg ccctcgacgc
     2101 gcgcggcttc gcagttgtcg caggcgcaga agggatcggg aaatccctcg gcggcggcgg
     2161 ttccgaggaa ggtcagcttc atggataact ccaggttggc gttcacgtgg gcaacgcgtt
     2221 ctgcccgatc accattgtgt tgtgagaagc cttacaggta ttaagacagt gccaacagat
     2281 tgtgaaagtc ctgagaggca aacttaaccg cgataacagt tcttgcctgg gacacccagt
     2341 cggccaccgc gtccgcgctg tcctccatct tctctcctcc caagcgtgtc gagggaccgc
     2401 tcgggcacca cgtgtcgtgc cgacaacgcc tcccaaggca cgtcctcccg agcccgtcga
     2461 cggaccgcgc aggcactccc atgcttcctg cgcaacgcct tctcaacagc gtcatcgcga
     2521 gcgagcgccg acgcgccgcc gtcaggcacg catccgtagg ggcgatcctc gtgatcaccc
     2581 gggtcgtccc gttcatgacg ccgcccctcc ttccggcgaa cgcgtcctgg aggggagcat
     2641 gtggacgccg gctgatccct cgataagccc gggaggacgt attttgcagg gcgttgcgtc
     2701 ggaaccgcgg ggacgaaacc ggtccctcga caggctcgga ggacgtgttg tgtagcgcgt
     2761 cgggaggacg cgtcccggaa cggcgacaag cgccgtgggg acgggaatgg gacgctgtaa
     2821 ccaaacttca cctccgggcg ttctgcttga tgagaggcac ccggattccg gcttttgagc
     2881 tttccccacc caaatctctt cgctccgcct ctcactggtt ctccctgggt cacggtccct
     2941 ccggttgtgg cggagggccg ccggcccggg gagccggttt tctggcaaat ggccggaatc
     3001 atcccgtctc atgggatgat gggtggcagt cagtcaaacg ttcaggcgac gacaacacga
     3061 cgcgcgggga tgagatcgac aatgcagacg aatgaagacc caggcgctac gatggtggca
     3121 tcgatggaac cggatcaacg cacccctatc ggtatcgatc cctcagggag gagttccatg
     3181 tctcacaccg agtttccccc cctgcgtgtg acgtcgcggc tcgctcgcct gcgcacgcgc
     3241 ttcgccgcgt cgaccggccg tcgccgatcc gaccgccgga tgcatgccgg tggccgcgcc
     3301 atccacggtg tcagcgcgat cgtcctcgtg ctcggtctct tcctgccgat gtacgcggct
     3361 ggaaacgtcg ccgcgtcacc gcagtcatcc tcccctgtcg atcccagcgc ccaggacagc
     3421 aacacggtga tcgccgcgcc gagccaggtc tcgatcacgg gcgatttcgg gcagttcgat
     3481 ctcaccagtt atgacggtgt ctggaagggc gtcttcgccg tccagcccgg tcaatacaat
     3541 taccagttcg tcgtgacc
//
LOCUS       DASTMD010000077         1520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1702991, whole genome shotgun sequence.
ACCESSION   DASTMD010000077 DASTMD010000000
VERSION     DASTMD010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702991"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFQ54_01155"
     CDS             complement(<1..309)
                     /locus_tag="VFQ54_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged-helix domain-containing protein"
                     /protein_id="HEU0163616.1"
                     /translation="MSLSPHTNGASLASSVVSDVARSALASELMPGALADADLAQIPD
                     IVIRRLPIYVRTLRALSERDVQSVSSEDLADLIGVTAAQIRRDLSYFGKFGKQGKGYD
                     T"
     gene            complement(591..1040)
                     /locus_tag="VFQ54_01160"
     CDS             complement(591..1040)
                     /locus_tag="VFQ54_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0163617.1"
                     /translation="MSSSSSDAGSSSYQSASGLSDVKIVEALAREFFLALDARDPRRL
                     RNTLSANATFRAHPHSAPIRPADEIVAYFGTVVSSYPNAHWDVTDVIATNDRGAVQFV
                     IREYSARLGRELISEQVAMIRVNAGKIVSLVGYYDSMEFQRVFWAGE"
     gene            complement(1147..>1520)
                     /locus_tag="VFQ54_01165"
     CDS             complement(1147..>1520)
                     /locus_tag="VFQ54_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0163618.1"
                     /translation="LISVKNDPDLDTIPPYQPAPAAYRSPRGRAPIELLAEFRRLRQA
                     TCSVLRSLPDDAWRRQGTSRLGYDVTIRGLAEGLAEHDYRYLRAMDQALDRSGAREGL
                     ATVQKAHLDELLRLVPETLKI"
BASE COUNT          292 a          487 c          469 g          272 t
ORIGIN      
        1 cgtgtcgtag cccttcccct gcttgccgaa cttcccgaag taggagagat cgcgacgaat
       61 ctgggcggcc gtgacaccga taaggtcggc aaggtcttcc gaggagaccg actgcacgtc
      121 acgctcggag agggcgcgca aggtccggac gtagatcggc agccgtcgga tcacgatgtc
      181 cgggatctgc gcgagatcgg catcggccag agcgccaggc atcaattcgc tggcgagggc
      241 actacgagcg acatccgaca ccaccgatga cgccaggctg gctccattgg tgtgcgggga
      301 cagagacaca tgatcccttg gcttcacggt tcaggctccc cgggaatgca ttgcgattga
      361 actggatggt cgggcgttgg gagccggcat cgagtctgcg gcgacggtca acttggccaa
      421 ccttgagtgg cggcggcgcg ggcaacaccc gaggatcgtg ccccatcaaa cgactggcaa
      481 tgcaccactt cattgtcagc cgaccgagct catcgctcat gccttacgcg acaagtgtac
      541 agactccgat tgtccggtcg ccactttttc gcaaacgacg ggcgttcgcg ctactctccg
      601 gcccagaaca cccgctgaaa ttccatactg tcgtaatacc cgacaagact cacgatcttt
      661 ccggcattga cccggatcat cgcgacctgt tccgagatca actcccggcc caatcgcgcc
      721 gagtactccc gaatcacgaa ttgcaccgcc ccccggtcgt tcgtcgcaat cacgtccgtg
      781 acgtcccaat gggcattcgg gtacgacgaa accaccgtcc cgaagtaggc gacgatctcg
      841 tccgcgggtc gaatcggagc ggaatgcgga tgcgcccgaa acgtggcgtt ggccgagagc
      901 gtgttccgca gccgtcgcgg gtcgcgggca tcgagggcga ggaagaactc gcgggccaat
      961 gcctcgacga tcttgacgtc ggaaagaccc gaagcgctct ggtatgacga tgatccggcg
     1021 tcggaagaag atgagctcat gaatatccga ttctgttctt gcctgaccgg cgcgactccg
     1081 tcgactcgag ccggggatca ggctgcgcga atcccaccca cacgattcag tttgcgtgga
     1141 atcgcgtcag atcttcaacg tctctgggac gagtcttaac agttcatcga ggtgcgcctt
     1201 ctggacggtc gcgagccctt cgcgcgcacc cgatcggtcg agtgcctgat ccatcgcccg
     1261 caggtagcga tagtcatgtt ccgccagccc ctccgccagc ccccgaatcg tcacgtcgta
     1321 cccgaggcgg ctcgtgccct gccgccgcca ggcgtcgtcc ggcaggctgc ggagcaccga
     1381 gcaggtcgcc tgccgcaacc ggcgaaactc ggcgagcaac tcgatcggcg cccgaccgcg
     1441 aggtgatcga tatgcggcgg gcgcgggctg atagggcggg atcgtgtcca gatcgggatc
     1501 gttcttcacg ctgatcaggt
//
LOCUS       DASTMD010000078         3958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_453652, whole genome shotgun sequence.
ACCESSION   DASTMD010000078 DASTMD010000000
VERSION     DASTMD010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453652"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFQ54_01170"
     CDS             <1..225
                     /locus_tag="VFQ54_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163619.1"
                     /translation="EQALKYPADVIFNSARPGTLTAEQLRAHPTWGLHPAVKAGQIGS
                     WNQDFIMSYQGMAAALEATIAPIRTAEKVT"
     gene            632..1366
                     /locus_tag="VFQ54_01175"
     CDS             632..1366
                     /locus_tag="VFQ54_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0163620.1"
                     /translation="MELAGKVATVSGGASGIGKATSSALAARGVKVVIADLNKDGGQA
                     TVDLIVENGGEAVFQYCDVTKTDDLVTLFDRAVSEYGSLDIAFNNAGIGEPDFFSDDL
                     GRWKRVIDINLIAVIEATRLAVLTMRRGGKPGAIVNTSSIVGLWPMPLSPVYAAAKAG
                     VIHFTRSLGYLKKDDEIRVNTICPELVDTPLVAPMGDEYLAKARAEGNILQPEEIAAA
                     VIELIEDESRAGAVMTIMTAEGRSYV"
     gene            complement(1357..1857)
                     /locus_tag="VFQ54_01180"
     CDS             complement(1357..1857)
                     /locus_tag="VFQ54_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0163621.1"
                     /translation="MSDDSQATLRKHVLALLAGRDARMTFDDAVVDFPAAHFNTKPEN
                     VPYTPWHILEHLRLTQLDILEFIRPEKYVEKAWPADYWPAHDAVTDQAGWDATIAGVQ
                     KDLAEIEAIVGDPGTDLFAVVPNGDAPHYTFLREANVVADHNSYHIGEFGILRQVMGT
                     WPDGHT"
     gene            complement(1910..2668)
                     /locus_tag="VFQ54_01185"
     CDS             complement(1910..2668)
                     /locus_tag="VFQ54_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU0163622.1"
                     /translation="MTSRRYFMLVIGHRGARNEAPENTLAGFAYAKRLGLDGVEFDVR
                     LSSDGQLVVIHDATVDRTTNGHGAVSDFTAVQLANLDARAQFPDWKGSCGVPLLTEVL
                     DVLGDDLRMMIEIKKDTPGNEERIIHGVMQELGLRGLMERATLTSFDTVALEIVQREF
                     PDQRRGYIGAWDSEEFIETAQRLGCAHGDAHRTSASPAVIAKAQDSGMTMLGWMCDTR
                     EEFDLLSAWGVDAVTTDNPGVILALRGALTTAAW"
     gene            complement(2702..3535)
                     /locus_tag="VFQ54_01190"
     CDS             complement(2702..3535)
                     /locus_tag="VFQ54_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEU0163623.1"
                     /translation="MSESSRQSISPSAGRPGALAAGYIFDLDGTIYLGDEVLPGAVET
                     IRTLRAAGRRVLFLSNNPTRAIDAYVAKLTGFGIPVTADEILTTLETTTRWLLRHAPG
                     KTVFPIGEAPLISALTAAGIRISEDPAEIDFVIASYDRGFDYRKLQIAFDAIHVHKRA
                     RLIATNPDRYCPFPGGRGEPDAAAIVGAIEGCTGARCEYVFGKPDPFMLDAAMQRLGL
                     SVDECMMIGDRLGTDIAMAVAAGMRSALVLTGETTPELLAASTIRPTWVLARIDDLIP
                     G"
BASE COUNT          749 a         1248 c         1269 g          692 t
ORIGIN      
        1 gagcaggcgc tgaagtaccc ggcggacgtc atcttcaact cggcgcgtcc gggaacgctg
       61 acggcggagc agctccgggc gcatccgacg tgggggctgc atcccgcggt caaggcgggt
      121 cagatcgggt cgtggaacca ggacttcatc atgagttacc agggaatggc ggccgcgctc
      181 gaagcgacga tcgccccgat ccggaccgcc gagaaggtca cgtagaacgg gatcgggggc
      241 gcccatcgcg tcccggggat gcaggatgtg cgtcggcacc cagttgcggc aagacacaag
      301 gtcttgcctt acaaacccga tgcgtcgcca agtcgcgggc cgctcccaac gctggtgcag
      361 agcgccgttc atgccggcat caggcgggac attcctccat ccagagaccg gacatcgctc
      421 cggcagacat cgcgatcgcg ggtctttgga ttccgcagtc tcgccctacg tacgaccgct
      481 gggcgaacaa tcctgtgcgg tcgagcgaca ccagtgaatc agtgcggatc gtggctacgt
      541 tcgcaatcgc gaagaagcaa tccgttcctt ctggtctatg atggcgatca ggatcatcct
      601 gatccagcgt cggcacagca agggatgttt catggaactg gcaggcaagg tcgcgacggt
      661 cagcggcggc gcatccggga ttggcaaggc cacttcatcg gcgctggcgg cacgtggcgt
      721 caaggtcgtg atcgccgatc tcaacaagga tggcggccag gcgacggtcg atctcatcgt
      781 cgaaaacggc ggtgaggcag tcttccagta ctgcgacgtg acgaagacgg acgatcttgt
      841 caccctcttc gatcgcgcag tcagcgaata cggatcgctc gacatcgcct tcaacaacgc
      901 aggcatcggc gagcccgatt tcttcagcga cgatctcggc cgctggaagc gcgtcatcga
      961 catcaatctc atcgccgtga tcgaggcgac ccggctcgct gtgctgacca tgcgtcgcgg
     1021 tggcaaaccg ggtgcgatcg tcaacaccag ctcgatcgtc ggactctggc cgatgccgct
     1081 gtcgccggtc tacgccgcgg cgaaggcggg cgtcatccac ttcacccgat cgctgggata
     1141 tctcaagaag gacgatgaaa tccgcgtcaa cacgatctgc ccggaactcg tcgatacccc
     1201 actggtggca ccaatgggtg acgagtacct tgccaaggca cgtgccgagg gaaacatcct
     1261 ccaaccggag gagatcgccg ccgcagtgat cgaactgatc gaggacgagt ctcgtgccgg
     1321 ggccgtgatg acgatcatga ccgccgaggg ccgctcctat gtgtgaccgt ccggccaggt
     1381 gcccatgacc tgacggagga tgccgaactc tccgatgtga tacgagttgt gatccgccac
     1441 cacgttcgcc tcccgcagga aggtgtagtg cggcgcgtcg ccgttgggca cgaccgcgaa
     1501 gagatcggtc cccggatcac caacgatcgc ctcgatctcc gccaggtcct tctggacgcc
     1561 ggcgatcgtc gcatcccacc cggcctggtc ggtcaccgca tcgtgagccg gccagtaatc
     1621 cgccggccac gccttctcga cgtacttctc cgggcggatg aactcgagga tgtcgagttg
     1681 tgtcaatcgc agatgctcga ggatgtgcca cggcgtgtag ggaacatttt ccggcttcgt
     1741 gttgaaatgg gcggccggga aatccacgac cgcatcgtcg aacgtcatgc gtgcatcacg
     1801 accggcgagc agcgcgagca cgtgcttgcg aagcgtggcc tgggaatcgt ctgacatcga
     1861 atggctcctt ccagcgaaag atcaatgcat ccatcctagc ggatgatcgt caccaggcgg
     1921 cggtcgtcaa ggctccgcgc aatgcgagga tcacgcccgg gttgtcggtc gtcaccgcat
     1981 cgacgcccca ggccgagagc agatcgaact cctcgcgggt gtcgcacatc cagccgagca
     2041 tcgtcatccc ggaatcctgc gccttcgcga tgacggccgg ggacgcgctc gtccggtggg
     2101 catcgccgtg ggcgcaaccg aggcgctgcg ccgtctcgat gaattcctcc gaatcccacg
     2161 cgccgatgta tccccgccgc tgatcgggga actcgcgctg cacgatctcg agggcgacgg
     2221 tgtcgaacga ggtaagcgtc gcccgctcca tgaggccgcg gagtcccaac tcctgcatga
     2281 cgccgtggat gatccgctcc tcgtttccag gggtgtcctt ctttatctcg atcatcatcc
     2341 ggaggtcatc gccgaggaca tcgaggactt ccgtgagcag cggcacgcca cacgatccct
     2401 tccagtccgg aaactgggcc cgtgcatcga gattcgcgag ctgaaccgcc gtgaagtcgc
     2461 tcacggcgcc gtgcccgttc gtcgtgcgat cgaccgtcgc atcgtgaatg accacgagtt
     2521 gcccgtcgga cgagaggcgg acatcgaact cgacgccgtc gaggccgagc cgcttcgcgt
     2581 aggcgaaacc ggcgagtgta ttctccggcg cctcattccg ggcgccacgg tgaccgatca
     2641 cgagcatgaa gtacctccgg gatgtcacga attctcgggt gaaactggct tcaggaacgg
     2701 gtcagccggg gatcaggtca tcgatcctgg ccagaaccca ggttgggcgg atcgtcgacg
     2761 cggccagcaa ttccggggtc gtctcgccgg tgagaacgag cgcagagcgc ataccagcgg
     2821 cgaccgccat cgcaatatcg gtcccgaggc gatcgccgat catcatgcat tcatcaacag
     2881 aaagaccgag ccgctgcatc gcggcgtcga gcatgaaggg atcgggcttg ccgaagacgt
     2941 attcgcaacg cgcgccggtg catccctcga tcgcgccgac gatcgccgcg gcgtccggct
     3001 cgccgcggcc acccggaaat gggcagtagc ggtcgggatt ggtcgcgatc aggcgtgccc
     3061 gcttgtggac atggatcgcg tcgaaggcga tctggagctt ccggtagtcg aagccgcgat
     3121 cgtagctcgc gatcacgaag tcgatctcgg cgggatcctc ggagatgcga attccggccg
     3181 ccgtgagcgc ggagatcagt ggcgcttccc cgatcgggaa gaccgtcttc cccggcgcat
     3241 gccggagcag ccagcgcgtg gtcgtctcga gtgtggtcag gatctcgtcc gcggtgaccg
     3301 ggattccgaa tccggtcagt ttcgcgacgt aggcatcgat tgcccgcgtc gggttgttcg
     3361 agaggaagag cacccgccga ccggccgcgc ggagcgtccg gatggtctcg accgcaccgg
     3421 gcaggacctc gtcgccgaga tagatcgtgc cgtcgagatc gaagatgtac ccggctgcca
     3481 gcgccccggg acgccccgcg gatggggata tcgattgtcg ggatgactcg ctcaccggga
     3541 ttcgtctttc ggactgtcag gaggacagga cgttccgtga gttaaccacg aatcccggaa
     3601 tctgagggag ttcgttctct gcaccgatgt gcgcgcgggc tgcgcgtggt cctgcgcaca
     3661 ttcgtgcagg caaagcatcg cagaacgttt acaatcggcc gaaccccaga aactatcaat
     3721 gaataagcga tacgcgttca ccacaggcgt agtcgtctta ccgatacacc ggctgtcccg
     3781 aagatcgatt cgaacgcacc aatcacaaaa ggggagaagt ccatgaagac gtcgatgtcc
     3841 cgacggcgat ttgccgtggc caccggtagc gccgctgtcg tcgcgcttgg cggcgcgaag
     3901 ctcgccggcg cgtcctcgtc gcgctacggc atgccggcag tcattcgcca gcagaatg
//
LOCUS       DASTMD010000079         1590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_7283, whole genome shotgun sequence.
ACCESSION   DASTMD010000079 DASTMD010000000
VERSION     DASTMD010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7283"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFQ54_01195"
     CDS             <1..423
                     /locus_tag="VFQ54_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163624.1"
                     /translation="PIMSVVAILRPAWQMRPVVRAVNPGAQRRQITVIGDLSGLDVGD
                     AEELGKLLGRGHGAIVMHGQPDERRLGAFRRGLTANEFPPVVIYLPTERKAINRALRT
                     ARKNDIVLMFPAGDPGPSIRAAARFDESALVSPDDSDD"
     gene            complement(1001..>1590)
                     /locus_tag="VFQ54_01200"
     CDS             complement(1001..>1590)
                     /locus_tag="VFQ54_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEU0163625.1"
                     /translation="IGDRIVGERIQVIGMITGVRRITTKTNRTMAVLEVEDLTGSIEI
                     VAFPDSFDQHGSRLEPDAILAFTAKIDERGESRQLILDSVTDELPEIKAVVTSGPTVA
                     IQLPATSDVWSDIDIMQSIERVLRRYEGEQPVVFLVPTQGGNLRLKSRTRRVEWCDEL
                     VRDLDGVLGSNMVTIERPKRAVAAVGDAELQAVAD"
BASE COUNT          281 a          542 c          488 g          279 t
ORIGIN      
        1 cccatcatgt ccgttgtggc gatactgcga cccgcgtggc agatgcggcc ggttgtacgc
       61 gccgtcaacc ccggcgccca gcggcggcag atcaccgtga tcggcgatct ctccggtctc
      121 gacgttggcg acgccgagga actgggcaag ctgctcggac ggggtcacgg cgcgatcgtg
      181 atgcacggac agcctgacga gcgacgcctc ggcgcgtttc gccgtggtct caccgcgaac
      241 gagttccccc cggtcgtgat ctacctgcca acggagcgga aggcgatcaa ccgggcattg
      301 cgaaccgcgc gcaagaacga catcgtcctc atgttcccgg ctggcgatcc ggggccgagc
      361 attcgcgccg cggcgcgctt cgacgaatcc gccctcgtct ccccggacga ctccgacgac
      421 tgaccgccct cccccgttgg gcagcacgaa atcgcacccg cgtccttgcg gaaccagctg
      481 tcgagcttcg tagctctatg ggatatgttg tgtagggata acctggagag cacgaacatc
      541 gtcgtgcttt agagcgaaat ccgggatcgt cgcgacgggg agctcgatcc gtgggcaaac
      601 gccaggcggg acaggccgtc cggccctccg gcccggcagc ggcggtcccg ctccttcgac
      661 aagctcagga cgggctcccg aacgaccggc tgctccgcgg aatacagatt cggtccagca
      721 gcaccttctg caaccgtttc ggatttcgcc gtagaaacgt cgcacacttc ctgcgtgact
      781 catgggatat gttgcgtcag catactcgcc caggggaaca gggctggcga cgcacgcggc
      841 accgactcgg caaagcgcgc ttgcttcgcc tgaagcgaaa tccggccgtc agcatattcg
      901 atggcgccgg ggttggtagg gcgggatttt ataccccgcc gcaaagcgaa ctcgcgggca
      961 gcacccaagg cgccgaatcg aacgcgccgt cccgctcggg ctaatcggcc acagcctgca
     1021 gttcggcgtc tccgaccgcg gcgaccgcgc gcttcggacg ctcgatcgtg accatgttcg
     1081 aaccgaggac gccatcaaga tcgcgcacga gttcgtcgca ccactccacg cgacgcgtcc
     1141 ggctcttgag tcgcaggttg cccccctgcg tcggaacaag gaagaccacc ggttgctcgc
     1201 cctcgtaacg gcgaagcaca cgctcgatcg actgcatgat gtcgatgtcg ctccagacat
     1261 ccgatgtcgc cgggagctga atcgcgaccg tcggcccgga cgtcacgact gccttgatct
     1321 cgggaagctc gtcggtcacc gaatcgagaa tcagctgcct gctctcccct cgctcgtcga
     1381 tcttcgccgt gaacgcgagg atcgcgtccg gttccagccg ggatccatgt tgatcgaagg
     1441 aatccgggaa ggccacgatt tcgatcgagc ccgtcaggtc ctcgacctcc agcacggcca
     1501 tcgttcgatt ggtcttcgtg gtgatccggc ggacgccggt gatcatcccg attacctgga
     1561 ttcgctcgcc aacgatccga tccccgatct
//
LOCUS       DASTMD010000080         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_721919, whole genome shotgun sequence.
ACCESSION   DASTMD010000080 DASTMD010000000
VERSION     DASTMD010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721919"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFQ54_01205"
     CDS             <1..305
                     /locus_tag="VFQ54_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163626.1"
                     /translation="GMSDKPVIARLTNGAEYGFASAELALKKHPDATIVSYQGGAPYE
                     AATAEKSVAEPPAAPTTSPPAADAAKGDNQPDAPETASAVDVASAPIPEHDGQAGG"
     gene            305..712
                     /locus_tag="VFQ54_01210"
     CDS             305..712
                     /locus_tag="VFQ54_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163627.1"
                     /translation="MATYDATTARALLERAIANTSKPALDDEAVDLLLQIAASEDGSY
                     STEALNSAAATGWGWKSGMLADQYNLGGGSGVYLTRSQWFEQCRQIQADFASGATSVD
                     GSGIVSENAMIGDVITISSDFLGGTDDRYEGWT"
     gene            709..1086
                     /locus_tag="VFQ54_01215"
     CDS             709..1086
                     /locus_tag="VFQ54_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163628.1"
                     /translation="MSTANDLDPVWADARAILADAFHTDTYEIWRPTDASNGRGGNAK
                     TLAQIESGDCVLRVDTNRSGQRVSSDLVVTTTAYVAELPIASMLMTTDTLKVNGRTFD
                     VQDVKRGGEWGLFTQVQMVEHMP"
     gene            1083..1427
                     /locus_tag="VFQ54_01220"
     CDS             1083..1427
                     /locus_tag="VFQ54_01220"
                     /inference="COORDINATES: protein motif:HMM:NF016754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEU0163629.1"
                     /translation="MSLLGKLTIEKNRFPQLVSAFPNAVAKLMNNAVMNVIYQADPLT
                     PVDTGALRANKTILLADPGKQEASVTWNQGYAVYQEFGTVHLEGAHFAQGAVDKITPG
                     YNADLGNLEGQL"
     gene            1427..1870
                     /locus_tag="VFQ54_01225"
     CDS             1427..1870
                     /locus_tag="VFQ54_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163630.1"
                     /translation="MFATRLVAPTIYEKLTTDPTISAAIGDRAFSTTTVPAGKAFPVA
                     LFYAEYSSYDGAIGTGLAGQLNMEQLRYTIRLICTGEDDTPILAAAEAQFAIFTKGRF
                     DISVDGVHYTISTRANSEVPLNTLLEGTDFYTQLGTTYDVTVVRG"
     gene            1884..>2367
                     /locus_tag="VFQ54_01230"
     CDS             1884..>2367
                     /locus_tag="VFQ54_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163631.1"
                     /translation="MVSTTNQIVQLQREASPGVLLKTAMKQYMALKLMPAWAVNGDSD
                     FKASGFKVNTAHIPGNVTGAWSVDGIQCYNAIGLAAACVLGYVPGTSTTTPEDATIAK
                     KHVFSPLAKGADPLSTYSVQYGEATKEAIEAAYLVFNSLGISVESGNLGFTSAAISKE
                     P"
BASE COUNT          471 a          753 c          770 g          373 t
ORIGIN      
        1 gcggcatgag cgacaagccc gtgatcgccc gcctcacgaa cggggcggaa tacggcttcg
       61 ccagtgctga gctggcgttg aagaagcacc cggacgcgac gatcgtcagc tatcagggcg
      121 gcgcgcctta cgaagctgcg actgccgaga agtcggtagc cgagccaccg gcggcgccga
      181 ccacatctcc gcccgcggca gatgcggcca agggtgacaa ccagccggac gcgccggaga
      241 cggcgtctgc ggttgatgtc gcctcggcgc cgatccccga gcacgacggc caggctggcg
      301 ggtaatggcg acctacgacg ccaccacagc ccgggcattg ctcgaacgcg ctatcgcgaa
      361 tacgagcaag ccggcactcg acgatgaggc ggtagacctg ctcctgcaga tcgccgcctc
      421 cgaggatggc agctattcga ccgaggcgtt gaacagcgct gcagcgacgg gctggggatg
      481 gaagtcgggg atgctcgccg atcagtacaa cctgggcgga ggctcgggag tctatctgac
      541 gcggtcacag tggttcgagc aatgtcgcca gatacaggct gatttcgcgt ccggagcgac
      601 ctcggtggat ggatcgggga tcgtgtccga gaacgcgatg atcggcgacg tgatcacgat
      661 cagcagcgac ttcctcggtg gcacggacga cagatacgag ggatggacgt gagcacggcg
      721 aacgatctcg atccggtgtg ggcagatgca cgggcgattc tcgccgatgc cttccacacc
      781 gacacctacg agatctggcg accgaccgac gcctcgaatg gccgcggcgg gaatgccaag
      841 acgctcgccc agatcgagtc cggcgattgc gtgctacgcg tcgataccaa tcgatcgggc
      901 cagcgcgtct cgtccgatct ggtcgtgacg accacggcgt acgtcgccga gttgccgatc
      961 gcgagcatgc tgatgacgac cgacaccctc aaggtcaacg gacgcacctt cgacgtccag
     1021 gacgtgaagc gcggcgggga atggggcctg ttcacgcagg tgcagatggt ggagcacatg
     1081 ccatgagcct gctcggaaag ctgaccatcg agaagaaccg gttcccgcag ctcgtcagcg
     1141 cgttcccgaa cgctgtcgcg aagctgatga acaacgccgt gatgaacgtg atctaccagg
     1201 cggatccgct caccccggtc gataccgggg cactgcgggc gaacaagacc atcctgctcg
     1261 ccgatccggg caagcaggaa gcctcggtga cctggaacca gggctacgcc gtctatcagg
     1321 agttcggcac ggttcacctc gagggagcgc atttcgcgca gggcgcggtc gacaagatca
     1381 cgcccggcta caacgccgat ctcggcaacc tcgaagggca gctctgatgt ttgcgactcg
     1441 cctcgtggcg ccgacgatct acgagaagct caccactgat ccgacgatct cggcggcaat
     1501 cggcgatcgc gccttcagca cgacgacggt cccggccggc aaggcctttc cggtcgcgct
     1561 cttctatgcg gagtattcga gctacgacgg ggcaatcggg accgggctcg ccggtcagct
     1621 caacatggag cagctgcgct acacgatccg gctgatctgc acgggcgagg acgatacgcc
     1681 gatcctcgcc gcggcggagg cccagttcgc gatcttcacc aaagggcggt tcgacatcag
     1741 cgtcgatggc gtccactaca caatctcgac ccgggcgaac agtgaggttc cgctgaacac
     1801 gctgctcgag ggaacggatt tctacacgca gctcggcacg acctacgacg tcaccgtcgt
     1861 acgcggataa cggaggatca ctcatggtgt ctacgacgaa tcagatcgtc cagctgcagc
     1921 gcgaggcgag tcctggcgtg ctgctcaaga cggcgatgaa gcagtatatg gcgctcaagc
     1981 tgatgccggc ctgggccgtc aatggcgact cggacttcaa ggcgagcggc ttcaaggtca
     2041 acaccgcgca cattccgggc aatgtcaccg gtgcgtggag cgtcgacggt atccagtgct
     2101 acaacgcaat cggcctcgcc gctgcctgcg tcctcggcta cgtcccgggc acgagcacca
     2161 cgaccccgga agacgcgacg atcgccaaga agcacgtctt ctcgccgctg gcgaagggcg
     2221 ccgatccgct ttcgacctat tccgtccagt acggcgaggc gacgaaggaa gcgatcgagg
     2281 cggcctatct cgtcttcaac tcgctcggga tctcggtcga atccgggaac ctcggcttca
     2341 cgtcggccgc gatctcgaag gagccgg
//
LOCUS       DASTMD010000081         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2864950, whole genome shotgun sequence.
ACCESSION   DASTMD010000081 DASTMD010000000
VERSION     DASTMD010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2864950"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFQ54_01235"
     CDS             complement(<1..569)
                     /locus_tag="VFQ54_01235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0163632.1"
                     /translation="MSTLVADASVEGRVRTFALTSLFLALFMELLDATIVNVALPSIE
                     KSLGASGGEQQWMIAAYTLALAVTLMTGARLGDIFGRKRLFIIGLVGFTVCSALCGLA
                     QNPGMLIASRALQGCAAAMMIPQVLSSIQVMYKPTERGAAMASFSALAGLAAVSGPIL
                     GALLTEADLFSWGWRTIFLVNIPVGIFSVV"
     gene            706..1242
                     /locus_tag="VFQ54_01240"
     CDS             706..1242
                     /locus_tag="VFQ54_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0163633.1"
                     /translation="MPRMSDFPPIHSNGHPFNRFPDEGSDPRAEFQEQVSRELAAQFA
                     AFMLLNVAIAEKVGIGVSDMHAMRIAWELGGCSIGTIGEQLHLTSGAVTKMVDRLVAG
                     GFVERLPDATDRRRAIVRAIPERLNEVGVAFDPLGARVMTFLEGLSPDQVAVIRAWTA
                     FSTEAALEESESLRSREE"
     gene            complement(1647..>2306)
                     /locus_tag="VFQ54_01245"
     CDS             complement(1647..>2306)
                     /locus_tag="VFQ54_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0163634.1"
                     /translation="DLIIRPTMNSIVIVAISVVGIVAFAAMSAYSFTFLQFPLRRLLF
                     TLVFVLLLVPGVLTLIPLYLQIKRLDFFGISNSWGGLILPYIAAGQAFAIFVLRTFFG
                     GISRDLIDAARVDGASEFHIFTRIVVPLSYPVIASVVVVNVVPLWNDYLLPSLLLEDK
                     YRTLTMALVSLQGNAQTHSSSQFGTLMAAYVIACVPLAVVFAFLMRYYVQGLTSGALK
                     L"
BASE COUNT          480 a          730 c          726 g          370 t
ORIGIN      
        1 acgacggaga agatgccgac cgggatgttg acgaggaaga tcgtccgcca gccccagctg
       61 aagagatcgg cctcggtcag gagcgcgccg agaatcggcc cggaaactgc ggcgagcccg
      121 gcgagcgccg agaatgacgc catcgccgcc ccgcgctcgg ttggcttgta catgacctga
      181 atactggaga gcacctgcgg gatcatcatc gccgcggcgc atccctgcag cgcgcgggac
      241 gcgatcagca tccccgggtt ctgggcgagg ccgcagagcg ccgaacacac cgtgaatccg
      301 acgaggccga tgatgaagag ccgctttcgt ccgaagatat ccccgagacg agcgccggtc
      361 atcaaggtca ccgcgagcgc gagcgtgtag gcggcgatca tccactgctg ctcgccaccg
      421 ctcgcgccga gcgacttctc gatcgaaggc agcgccacat tgacgatcgt ggcgtccagc
      481 aactccatga agagggccag gaagagactg gtcaaggcaa atgtgcgaac tcttccttcg
      541 acggaagcat ccgcgaccaa cgtggacatg tataccttct cccgcaaacc aacaaaccct
      601 atccgtacgt acatcgcttg cgtagtatct tactctggaa gatacttcca gtgtcaagca
      661 tgttgatcag aggttcacga tcgttcgcca aggatccagt cgccgatgcc gagaatgtcg
      721 gattttccac ccatccactc caatggccat cccttcaaca ggtttccgga cgaaggctcg
      781 gacccccgcg ccgagtttca ggaacaggtc tcgcgtgaac tcgccgcgca attcgccgcc
      841 ttcatgctgc tcaacgtcgc gatcgcggag aaggtgggga tcggcgtgtc ggatatgcac
      901 gcgatgcgga tcgcctggga gctcggcggt tgcagtatcg gcacgatcgg cgagcagctt
      961 cacctgacca gcggcgcggt cacgaagatg gtcgaccgtc tcgtcgccgg aggattcgtc
     1021 gaacgcctgc cggatgccac cgatcgccgc cgggcgatcg tccgcgcgat tccggaacgg
     1081 ttgaacgagg tcggcgtcgc cttcgatccg ctcggcgcca gggtgatgac cttcctcgag
     1141 ggattgagcc ccgatcaggt cgcggtgatc cgtgcgtgga cggcgttctc gaccgaggcc
     1201 gcgctcgaag agtcggaatc actccgctcc cgcgaagaat gacgccgggc gagtctcgcc
     1261 gaattcccgt cgggaccggt cgcgccatag gcgtgtcgat cgggccgttc gtgtttgcgg
     1321 cggggtatga aaccccgccc taccaacccg gtcggttcca acgaccttcc acgcctatgg
     1381 aacatcaacc gcaatcacgg atgccgcggt cgtgcgtacg gcgggacacg gggtcccgcc
     1441 ccgacctccc ggttcccgtc ccggcgaccc acgtggtcgc ctcgggtcga cagtgtcgac
     1501 ccgattccgg ggggcgtctt gtgccgaatg ttcccgtcag gatccgccgg cgccggatgc
     1561 actccgcacc gggatttctc gacaggctcg aaacgacgaa ccttcaagcg aaagcgttga
     1621 tccgacagac cccacgatgc cgcgcatcac aacttgaggg caccgctcgt cagtccctgg
     1681 acgtagtacc gcatcaggaa ggcgaagacg accgcgagcg ggacgcaggc gatcacgtag
     1741 gccgccatca acgtgccgaa ctgggatgac gaatgcgtct gcgcgttgcc ctggagactg
     1801 acgagcgcca tcgtcaatgt ccggtacttg tcttcgagca gaagcgacgg gagaagatag
     1861 tcgttccaga gcggcacgac gttgacgacc acgacgctcg cgatcaccgg gtacgaaagc
     1921 ggcacgacga ttcgcgtgaa gatgtggaac tcgctcgccc catcgacccg cgcggcatcg
     1981 atcagatccc gcgagatacc accgaagaag gtgcggagaa cgaagatcgc aaacgcctga
     2041 ccggcagcga tgtacggcag gatcaggcca ccccagctgt tggaaatccc gaagaagtcg
     2101 agccgcttga tctgcaggta gagcgggatc agtgtcagca cgccgggaac cagcaggagc
     2161 acaaagacga gtgtgaagag caggcggcgc agcggaaact gcaggaaggt gaacgagtag
     2221 gcgctcatcg ccgcgaaggc gacgatcccg accacgctga tcgccacgat cacgatcgag
     2281 ttcatcgtcg gccggatgat gaggtc
//
LOCUS       DASTMD010000082         1972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3132710, whole genome shotgun sequence.
ACCESSION   DASTMD010000082 DASTMD010000000
VERSION     DASTMD010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3132710"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1010)
                     /locus_tag="VFQ54_01250"
     CDS             complement(141..1010)
                     /locus_tag="VFQ54_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0163635.1"
                     /translation="MSASTPPIPGEVRVLRAKKLAITAWRELAPVIVTILFLMPFIVM
                     LSTSFKSADETFALPPRLWSSHWVTSNYHAVFVEMPFWRYLWNTIFLSIMTVICTLFS
                     CPLVAYSLAKVPWKGRGPLFILILSTMMLPPQVTMIPIYIMWNQTPLMGTYWPLIVPN
                     FLGSAFSIFLLRQFFRGIPNDLLDAARLDGASEIRIYWNVVLPLARPALATIGLFAFV
                     GAWTDFLNPLIYLSDPNKYTLSIGLYNFFSEHGVEWGPLMAACAIFSIPLIVIFIFAQ
                     RQFIEGISLTGLK"
     gene            complement(1013..1930)
                     /locus_tag="VFQ54_01255"
     CDS             complement(1013..1930)
                     /locus_tag="VFQ54_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0163636.1"
                     /translation="MRTLRLPSRTLSPSTVGVLFALPWIIGFLVFQLYPIGASANYSF
                     TDYSLFTSPNWIGLANYRKLIHDDLFYKSVYNTLYMTVIGVPLGLCFALICALALNTP
                     SKLQPFYRAMVYLPTVVPIVAATYLWRWILNSQYGYVNSALGGVGLTQPLWQEDAAWT
                     KPALLLIGLWTVGGTAIIYLAALKEVPRSYYEAAEIDGANWWSKFWNITWPAISPVTL
                     FQLVIGVIGSLQYFTQAYLFAQQRMNDASGGPKNSLLMYGMYIFQNAFSYIDMGYASA
                     LSWVLFLISLALTAGILGMANRWVHYEGD"
BASE COUNT          421 a          547 c          673 g          331 t
ORIGIN      
        1 cgactgagga ccggctctgc cggtcccgcg tctacggttt cgggtacgga tctgcgagtg
       61 cggcgtggcc aatgccggca tcgtatcgac ctgcggtcga tgggtcacac ggggtgaccc
      121 gctacgaaat tcgatggcca ctacttcaag ccggtgaggg agattccctc gatgaactgg
      181 cgttgggcga agatgaagat cacgatcagc gggatgctga agatcgcgca ggcggccatc
      241 aacggacccc actccacacc gtgctccgag aagaagttgt agagtccgat cgagagcgtg
      301 tacttgttcg gatcgctcag gtagatcagc gggttcagga aatcggtcca ggctccgacg
      361 aaggcgaaga gcccgatcgt tgccagcgcc ggtcgcgcaa gcgggaggac cacgttccag
      421 tagatgcgga tctccgaggc gccgtcgagg cgggccgcgt cgagcagatc gttcgggatg
      481 ccgcggaaga actgccgcag caggaagatc gagaaggcgg atcccaggaa gttcggcacg
      541 atcagcggcc agtaggttcc catcagcggc gtctggttcc acatgatgta gatcgggatc
      601 atcgtcactt gcggcggcag catcatcgtc gagaggatga ggatgaagag cggaccgcgt
      661 cccttccacg gcaccttcgc gagcgagtag gcgacgagcg ggcaggagaa gagcgtgcag
      721 atcacggtca tgatgctgag gaagatcgtg ttccagaggt aacgccagaa cggcatctcg
      781 acgaagacgg cgtgatagtt cgaggtgacc cagtggctgc tccagagtcg gggcggcagc
      841 gcaaaggtct cgtccgccga tttgaacgac gtgctcaaca tgacgatgaa gggcatgagg
      901 aagagaatcg tcacgatcac cggggcaagc tcgcgccagg cggtgatcgc gagcttcttc
      961 gcccggagga cgcgaacctc gccaggtatc ggaggggttg atgcggacac gatcaatctc
     1021 cttcgtagtg gacccagcga ttcgccatgc ccaggattcc ggccgtcagg gccagcgaga
     1081 tcaggaagag cacccacgac aacgcagagg cgtatcccat gtcgatgtag gagaaggcgt
     1141 tctggaagat gtacatgccg tacatcagca gggagttctt cgggccgccg ctcgcgtcat
     1201 tcatccgttg ctgcgcgaag aggtaggcct gcgtgaagta ctggagcgag ccgatgacac
     1261 cgatcacgag ctggaagagc gtgacgggcg agatcgccgg ccaggtgatg ttccagaact
     1321 tgctccacca gttggcgccg tcgatctccg ccgcctcgta gtaggagcgc gggacctcct
     1381 tcaacgccgc gaggtagatg atcgcggtgc cgcccaccgt ccagagcccg atcaggagca
     1441 gcgccggctt cgtccaggcc gcgtcctcct gccagagcgg ctgcgtcagt ccgacgccac
     1501 cgagcgcgga gttgacatag ccatactgcg agttgaggat ccagcgccag agataggtcg
     1561 ccgcgacgat cgggaccacg gtcgggaggt aaaccatcgc gcggtagaag ggctgcaact
     1621 tcgacggcgt gttcagcgca agcgcgcaga tcaacgcgaa gcagagcccg agcggcacgc
     1681 cgatcacggt catgtagagc gtgttgtaga cgctcttgta gaagagatcg tcgtggatca
     1741 gcttccggta attcgccagg ccgatccagt tcggcgaggt gaagagactg tagtcggtga
     1801 agctgtagtt ggcggatgcg ccgatcggat agagctggaa caccaggaag ccgatgatcc
     1861 aggggagtgc gaagagcact cccacggtcg aaggcgaaag ggttcggctc ggcagccgca
     1921 gagttcgcat gacgtgtcct gacgatgacg gttggttcct tgaatgcgcc gc
//
LOCUS       DASTMD010000083         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_544300, whole genome shotgun sequence.
ACCESSION   DASTMD010000083 DASTMD010000000
VERSION     DASTMD010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544300"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1395)
                     /locus_tag="VFQ54_01260"
     CDS             complement(97..1395)
                     /locus_tag="VFQ54_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0163637.1"
                     /translation="MELSRIWLVARREWTTRVQQRSFRIVTVIQILLIVVGACLPTIF
                     GRFTNDNGKTDHIVVVDEANIGAASKLAPYFAVSGDTSGVDRIVIDPAPANADQARQW
                     VNEDEIDGALIVSRDQSGNLAFVLETKSGDTSTTSQQVNAAASALSIQDRLAQAGVSQ
                     QAFDQATAPPAFALQGTDTKSSSDNANDGARYGVGFVFTILMFMAIMLYGQWVAQGVV
                     EEKSSRIMEIMVNAATPRDLLFGKVIGIALAGFTQLVPMMVAGGITFAAQPRIADALN
                     VKDSDITAIDFSSISVSAVGSFAVFFLIGFTLYASLFASVGSLVSRQEEVSQAVSPLT
                     TVMMVGYLGGLFTVGSPDSLFARIISIFPLTAPFSMVMRIVVGNPSGWEIALSIGLLA
                     ITAVGGVMFAARVYRVGVLMYGQKPSWRAVFNPNAVRAAR"
     gene            complement(1449..2411)
                     /locus_tag="VFQ54_01265"
     CDS             complement(1449..2411)
                     /locus_tag="VFQ54_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0163638.1"
                     /translation="MALTIDHITKHFGNFTAVDDVAFTVTPGRIFGFLGTNGAGKTTT
                     MRMVLDIIRPDKGSITWNGMPNTDVPRREFGYLPEERGLYPKMPVDDQLLFLAQLYGA
                     SKQQAAKELDEWLERLEVTENKHKRVEQLSKGNQQKIQFLAAILHDPEILILDEPFSG
                     LDPVNAEQMKQAFLTMKDRGKTIIFSTHQLDDAQELCHDVAIIHRGHLVISGDVPSVR
                     QSAGQQIVRLAIDGDSQVAWLDQMERVQVLRRREDFVEMSVPSEQEARAVLSEAMRRE
                     LPVTRFEISYPSLNDIFLGAVRKLPFQEQQIPAVREFAPEPIVV"
BASE COUNT          486 a          866 c          764 g          473 t
ORIGIN      
        1 cccttgggaa gaagaggcgg gaccgccgct gccggacctg ctgtccggac ggcccgtccc
       61 gcctctcttc tcccgatgcg tattcctcgc cgtcccctaa cgcgccgcgc ggaccgcgtt
      121 cggattgaag acggcgcgcc aactcggctt ctggccatac atcagcacgc cgacccggta
      181 gacgcgtgcc gcgaacatca cgccgccaac cgccgtgatc gccagcaacc cgatgctgag
      241 cgcgatctcc cagccactcg ggttgcccac gacgatccgc atcaccatgc tgaacggcgc
      301 cgtcagcggg aagatcgaga tgatccgggc gaagaggctg tcaggcgatc ccaccgtgaa
      361 gagcccgccg agatagccga ccatcatcac cgtcgtcaac ggcgagacgg cctggctcac
      421 ctcttcctgc cggctcacga gggagccgac agaggcgaag agcgaggcgt agagcgtgaa
      481 gccgatcagg aagaagaccg cgaacgagcc aaccgcggag accgagatcg aactgaagtc
      541 gatcgcggtg atatcgctgt ccttcacgtt gagcgcgtcc gcgatccgcg gttgcgcggc
      601 gaaggtgatg ccaccggcga ccatcatcgg gacaagctgg gtgaaccccg ccagggcgat
      661 cccgatcacc ttcccgaaga gcaggtcccg tggcgtcgcc gcgttgacca tgatctccat
      721 gatccgcgaa ctcttttcct cgacgacacc ctgcgcgacc cactgcccgt aaagcatgat
      781 cgccatgaac atcaggatcg tgaagacgaa gccgacgcca tagcgtgcgc catcgttcgc
      841 gttgtcactt gagctcttcg tatcggtccc ctgcagggca aacgcgggag gtgccgtcgc
      901 ctgatcgaac gcctgctgcg aaacacccgc ctgcgcgagg cgatcctgga tgctgagggc
      961 ggaggcagcg gcgttgacct gctgcgaggt cgtgctcgtg tccccggatt tggtctccag
     1021 cacgaacgcg agattgcctg attgatcgcg ggagacgatc agcgcgccat cgatctcatc
     1081 ctcgttgacc cattgccgcg cctggtcggc gttcgccggc gccggatcga tcacgatccg
     1141 atccacaccg ctcgtatcgc cgctcaccgc aaagtacggt gcgagcttgc tcgcagcgcc
     1201 gatgttcgcc tcgtcgacaa cgacgatgtg atcggttttc ccgttgtcgt tggtgaaacg
     1261 gccaaagatc gtgggcaggc aggccccgac aacgatcagc aggatctgga tcacggtcac
     1321 gatccggaag cttcgctgct gcacccgggt cgtccactcg cgccgggcga ccagccagat
     1381 tctcgacaac tccatggttc cgtcttcctc aaccagccaa cacaggaaaa tctcgacgtt
     1441 tgcacgcgct agacgacgat cggctccggc gcaaactccc ggaccgcggg aatctgctgc
     1501 tcctggaatg gtagcttccg gacagccccg aggaagatgt cattcaacga cgggtagctg
     1561 atctcgaagc gcgtcaccgg caactcccgc cgcatcgcct ccgagagcac cgcccgcgcc
     1621 tcctgctcgg acggcacgga catctcgacg aaatcctcac ggcgtcgcag cacctgcacg
     1681 cgttccatct ggtcgagcca ggccacctgg gaatcgccgt cgatcgcaag ccggacgatc
     1741 tgctgccccg cgctctgccg gaccgacggc acatcgcccg agatcacgag atggccgcga
     1801 tgaatgatcg cgacgtcatg gcagagctcc tgggcgtcat cgagctggtg ggtcgagaag
     1861 atgatcgtct tgccgcgatc cttcatcgtc aggaatgcct gcttcatctg ctcggcattg
     1921 acgggatcga gcccactgaa cggctcgtcg aggatgagga tctcgggatc gtggaggatc
     1981 gccgcgagga actggatctt ttgctgattg cccttgctca gctgttcgac ccgcttgtgc
     2041 ttgttctccg ttacctcgag tcgctccagc cactcgtcca gctccttcgc cgcctgctgc
     2101 ttcgaggcgc cgtagagctg ggcgaggaag agaagctgat cgtcgaccgg catcttcggg
     2161 tagagaccgc gctcctccgg caggtagccg aactcgcggc gcgggacgtc cgtattcggc
     2221 atcccattcc acgtgatcga gcccttgtcc ggccggatga tgtcgagcac catccgcatc
     2281 gtggtggtct tgcctgctcc gttcgtcccg agaaatccga agatccggcc cggagtcacc
     2341 gtgaatgcga cgtcatcgac tgccgtgaag ttcccgaaat gcttggtaat gtgatcgatc
     2401 gtcaaggcca tggacagcgc cccctcatct cgcgcggcgg tcgatcgtgc gggcacggaa
     2461 tccggatcca ccacgcttct gccgcgacgg cacaacattc catctcgccg tattcgtaga
     2521 agtatcaaac ccgattgtca cgaattctcg aacacggggt gacatcctgt attcatccac
     2581 ccattcttt
//
LOCUS       DASTMD010000084         1610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1883149, whole genome shotgun sequence.
ACCESSION   DASTMD010000084 DASTMD010000000
VERSION     DASTMD010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883149"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VFQ54_01270"
     CDS             <1..885
                     /locus_tag="VFQ54_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163639.1"
                     /translation="PLATSFASPETTLAAAAASSSCKGIATWYPKVDGLAAGIMKDEA
                     SNTATSDKYRTLVSELKDANVSAAKDASTSLEEGYTELASYLEDSSTLGQLGAGFSDY
                     ATAIENLSDARHKILSGYLDWSKLGVDCGLVTKFQVKDRKGCGGTTDFLTRAAADYTS
                     WWQFWTVATSQVQRDAEQPTPNSSAGEAMSTPESLSALAYEAALDLAVAPASKYVLDL
                     QKEIVQNFLDLSDLYAIEMISLWGGLLGVDAATQNQITQQFSDQSNLTIKEHTDIDTA
                     WSKRAKSCGVDVAFPALP"
     gene            977..>1610
                     /locus_tag="VFQ54_01275"
     CDS             977..>1610
                     /locus_tag="VFQ54_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0163640.1"
                     /translation="MMGDLLPGERIRRIRLELGLSQVELAGLLGVSNVTVNRWEHDKS
                     TPQPETRSRLLRAEVLGIGALQARPVAVASAPARSGPGTSDLVGRERDLARLLVELQP
                     GRVVTIVGPGGVGKTSLALEAGDLLADRFPDGIWFVDLTSLGVRQSITDEVARVMGIR
                     AATSAKLAERVRSRLATGAALVVLDNCEHVTAGCREFIDSCFDDSGQAAIF"
BASE COUNT          288 a          500 c          533 g          289 t
ORIGIN      
        1 ccgctggcga cgtcgttcgc gtcgcctgaa acgacactcg ccgcggccgc ggcgtcatcc
       61 tcctgcaagg ggatcgcgac gtggtatccg aaagtcgatg gtctcgcggc gggcatcatg
      121 aaggacgagg cgagcaacac cgcgacatcc gacaaatatc gcacgctggt ctcggagctc
      181 aaggatgcga acgtctccgc cgcgaaggac gccagcacgt cgctcgaaga gggctacacc
      241 gagctggcat cctaccttga ggactcgtcg acactcggcc agctcggcgc gggattctcg
      301 gactacgcga cggcgatcga aaatctcagc gatgccaggc acaagatcct gagtggttat
      361 ctcgattggt cgaagctcgg tgtcgattgc gggctggtca cgaagttcca ggtcaaggac
      421 cgcaagggat gtggcggcac gaccgacttt ctcacccgtg cagcggccga ctatacctcg
      481 tggtggcagt tctggaccgt ggcgacctcg caggtgcagc gcgatgccga gcagccgacg
      541 ccgaattcga gcgccggtga ggcaatgtcc acgccagaga gtctttcggc gcttgcgtac
      601 gaagccgcac ttgatcttgc cgtggcgccg gcttcgaaat acgtgctcga tctccagaag
      661 gagatcgtcc agaacttcct cgatctctcc gacctctacg caatcgagat gatcagtctc
      721 tggggcggat tgctcggcgt ggacgccgcg acccagaacc agatcacgca gcaattcagc
      781 gatcagtcga acctgacgat caaggaacat accgacatcg acactgcctg gtcgaagcgg
      841 gcgaaatcgt gcggcgtaga tgtcgcgttc cccgctctgc cgtgatcgga ggtcggcgat
      901 cgcagcttct cgggaagttc acagtatgat ggagtccacg ggacctgctt atcggcgctt
      961 gacctgacga tcgaagatga tgggcgacct gcttcccggg gaacgcattc gaaggatcag
     1021 gcttgagctc ggcctctccc aggtcgagct cgcggggctt cttggtgtct ccaacgtcac
     1081 cgtgaaccgg tgggagcatg acaagtcgac accgcagcca gagacgcgga gccggttgct
     1141 ccgggcggag gtcctcggga tcggggcgct gcaggcgcga ccagttgccg tcgcttcggc
     1201 gcccgctcgc tccggaccgg gtacgagtga tctcgtcggt cgcgagcgcg atctggcgag
     1261 gctgctggtc gaactccagc ccggccgcgt cgtgacgatc gtcgggccgg gcggggtcgg
     1321 caagacgagc ctcgcgctcg aggcggggga cctcctcgcg gatcgctttc ccgacggcat
     1381 ctggttcgtg gacctcacgt cgttgggcgt ccgtcaatcg atcacggacg aggtggcgcg
     1441 cgtcatggga attcgcgccg caacgagtgc caagctcgcg gagcgcgtgc gttcccgcct
     1501 cgcgacggga gcggcgctcg tcgttctcga caactgcgag catgtcaccg ccgggtgccg
     1561 ggagttcatc gattcctgct tcgatgacag tggccaggcg gccatcttcg
//
LOCUS       DASTMD010000085         4194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1526187, whole genome shotgun sequence.
ACCESSION   DASTMD010000085 DASTMD010000000
VERSION     DASTMD010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526187"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFQ54_01280"
     CDS             complement(<1..186)
                     /locus_tag="VFQ54_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163641.1"
                     /translation="MIRVRHATVDDAPRIAEIHVRTWQSAYRGQLPGVFLDGLEPAQR
                     EPGWKRTIAESPSNVVVL"
     gene            complement(188..751)
                     /locus_tag="VFQ54_01285"
     CDS             complement(188..751)
                     /locus_tag="VFQ54_01285"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0163642.1"
                     /translation="MSASTPTFTIRRAQLADAPVIADIHVRAWQTTYKGIMPDDFLAG
                     LAPEQRYARWERDITVPDREMTVIVAVEADSDRVAGFCSVCPQKHLEPGDESLPPAGE
                     LHTMYVDTGFKGGGMGRALITTGVDALRDLGYTRAVVWMLADNAPARAFYERMGWTVD
                     GVSSFVRYGDGQIREIRLARSLVDADS"
     gene            complement(748..1944)
                     /locus_tag="VFQ54_01290"
     CDS             complement(748..1944)
                     /locus_tag="VFQ54_01290"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEU0163643.1"
                     /translation="MNRIVRRDQESTHQAANVLELFYDLVFVFAITQVSHLLLSHLNW
                     TGVVQSVIALMAVWWSWNYTTWFTDEVDPESNWIRIFLLALMLLSLLMAVALPHAFGD
                     RATLFAVAYVAIQVGRHGFLTFVAAGPASIERERAGRILAWFVVAGIFWIGGSFADGW
                     TQIWLWIIAVAIDYGAPLATFWLPGRPRLVGDNWQVAPEHFTERFQLFILLALGESIV
                     VTGSTTSDLDLTTSRVFAFAISFIGSAALWWLYFSSGAKYSYQYLEYSDDPTILARDA
                     YTYLHLVLAGSIILSAVGDEFAIAHPTHHLTHPQLAVVVLGPAIYLLAQVLFAWRMAG
                     IVSWIRVLGIVGCLVVGLIGLSTPAIVVAALLVVVQIAVIVAQEFVPSQRFGPHPAFA
                     AGHHAA"
     gene            complement(1991..2557)
                     /locus_tag="VFQ54_01295"
     CDS             complement(1991..2557)
                     /locus_tag="VFQ54_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0163644.1"
                     /translation="MDNRVFAWFEGTSRMPIPSSRRTPNRPHRQVWAAYLHSQTKLYR
                     LLERELQEAHGISMAEYDVLAQLIVSPERRIRIKELAGRILYSTGGLSRLLDRMEISG
                     YVQRVRTPEDRRGVFAAITDEGITTIRAAGRTHLAGVQRHFAAKLHDDEFDAVKAFLD
                     RLMEDSEDDIQQAQALGNGTSAARDRIR"
     gene            2653..3561
                     /locus_tag="VFQ54_01300"
     CDS             2653..3561
                     /locus_tag="VFQ54_01300"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU0163645.1"
                     /translation="MADATITPVSPSTVQLTPRTVSRRWALGDPRPTSQSDRARMVLD
                     LMDPAVTDPFVVLAEDWFSEVGFDWHPHRGFETVTYVIDGELEHKDSTGGHAVLTPGD
                     VQWTTMGHAVLHAELAYQKKGVHTLQLWLNSPAALKMIEPGYQNLSASTVPTIERDGA
                     RVRVYAGSVQGVTGPAATIWPATVIDARLSAGGHFTHELPSTFAGLMYVIEGKVRAGS
                     DGTVVATGEAAWFDHIDLREDGRAPSATGIEIVADEETLLIFYAAAPILEPVVAYGPF
                     VMNTREEIIQTYEDYQSGKFPAYPNW"
     gene            3605..4117
                     /locus_tag="VFQ54_01305"
     CDS             3605..4117
                     /locus_tag="VFQ54_01305"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU0163646.1"
                     /translation="MNVNEAIRTVLAVRAFQDKPVPAGIRQEILEAAHLSASGSNKQP
                     WHFIAIEDPERIKGLARFTPTGPYVAEAPFVVVVAAKKGLNGLSDASRAIQDMILVAW
                     SEGVGSNWVGWGNTLDGVKEFVGIPSESDLEIIAVLPFGYPVESVGKGKKNRKPFDEV
                     IHRETFSGVA"
BASE COUNT          817 a         1339 c         1339 g          699 t
ORIGIN      
        1 aagcaccaca acgttcgacg gtgattccgc gatcgtacgc ttccagccag gctcgcgctg
       61 ggcgggctcg agaccatcga ggaagacgcc tggaagctgt ccccggtagg ccgactgcca
      121 ggtgcggaca tgaatctcgg cgatcctcgg tgcatcgtcg accgtcgcat gacgaacgcg
      181 gatcatgtca ggaatccgca tcaacgagcg atcgtgcgag gcggatctcg cgaatctgcc
      241 cgtcgccata ccggacgaag gaactcacgc cgtcgaccgt ccatcccatc cgctcgtaga
      301 acgcccgggc cggggcgttg tccgccagca tccagacgac ggcccgggtg tatccaaggt
      361 cgcgcaaggc atcgacaccg gtggtaatca gggcacgccc catcccgcct cccttgaacc
      421 cggtgtcgac atacatcgta tgcagctcgc cggcgggagg aagggattcg tcccccggtt
      481 cgagatgctt ctgcggacag acgctgcaga agcccgcgac acgatcgctg tccgcttcga
      541 cggcgacgat cacggtcatc tcgcgatcgg gaacggtgat gtcgcgctcc caccgcgcat
      601 atcgctgctc cggggcgagc ccggcgagga agtcgtccgg catgatcccc ttgtaggtcg
      661 tctgccacgc ccggacatgg atatccgcga tgaccggcgc atcagccagt tgggcgcggc
      721 gaatcgtgaa cgttggcgtc gatgcactca tgcggcgtga tgtcctgcgg cgaaggcggg
      781 atgtggaccg aatcgctgcg acggcacgaa ttcctgggcg acgatcaccg cgatctggac
      841 gacgaccagc agcgccgcga cgacgattgc cggggtcgaa agcccgatta atccgacgac
      901 gaggcatcca acgatcccca gcacacgaat ccagctcacg atcccggcca tccgccacgc
      961 gaagagcacc tgcgcgagca ggtagatcgc aggccccagg acgacgacgg cgagttgcgg
     1021 gtgagtcaga tggtgcgtgg gatgcgcgat cgcgaattcg tcaccaaccg cgctgaggat
     1081 gatcgagccg gccagcacga gatgcaggta ggtataggca tcacgcgcga ggatggtggg
     1141 gtcgtcggaa tactcgagat actggtacga atatttcgcg cccgacgaga aatagagcca
     1201 ccagagcgcg gcgctcccga tgaaggagat cgcgaaggcg aagacgcggg acgtcgtcag
     1261 atcgagatcc gaggtcgtac tcccggtgac gacgatcgat tcgccgagcg cgagcaggat
     1321 gaagagctgg aagcgttcgg tgaaatgctc gggcgcgacc tgccagttgt cgccaacgag
     1381 ccgcggtcgc ccgggaagcc agaaggtggc gaggggtgcg ccgtaatcga tcgccaccgc
     1441 gatgatccag agccagatct gggtccagcc gtcagcaaac gaaccgccga tccagaagat
     1501 tccggcgacg acgaaccagg cgaggatgcg cccggcccgc tcgcgctcga tgctggcggg
     1561 accagcggcg acgaacgtga ggaatccgtg ccgcccgacc tggatcgcga cgtaggcgac
     1621 ggcgaagagt gtcgcgcgat cgccgaaggc gtgcggcaac gccaccgcca tcagcaggct
     1681 gaggagcatc agcgcgagca ggaagatccg gatccagttc gattccgggt cgacctcgtc
     1741 cgtgaaccag gtcgtgtagt tccacgacca ccagaccgcc atcaacgcga tcaccgactg
     1801 gacgaccccg gtccagttca gatgggacag aagcaggtgc gagacctgcg tgatcgcgaa
     1861 gacgaagacg agatcgtaga agagttccag gacattcgcc gcctgatgcg tgctctcctg
     1921 gtcgcgccga acgatgcggt tcatctttcc tcccccgaga agcggattcc ccaaccgata
     1981 tcagttcatc ctagcggatt cggtctctcg cggcactcgt gccgttgccc aaagcctgtg
     2041 cctgctggat gtcgtcctcc gaatcctcca tcaggcgatc gaggaacgcc ttgacggcat
     2101 cgaactcgtc gtcgtggagc ttcgcggcga agtgacgctg gaccccggcg agatgggtgc
     2161 gaccggcggc gcggatcgtg gtgatcccct catcggtgat cgcggcgaag accccgcggc
     2221 gatcctccgg tgttcgcacg cgctgcacgt agccggatat ctccatccga tcgagcaggc
     2281 gactgagccc accggtgctg tagagaatcc tcccggcgag ttccttgatc cggattcgcc
     2341 gctccggcga gacgatcaac tgggcgagca cgtcgtattc cgccatcgaa atgccatgtg
     2401 cctcctgcag ttcgcgctcg agcaggcgat acagcttcgt ctggctgtga agatacgccg
     2461 cccacacctg gcgatgcgga cgattcggtg tgcggcgtga cgacgggatt ggcatccggg
     2521 aggtcccttc gaaccaggcg aaaacgcgat tgtccacgca ttgtagtttc tctggaagtc
     2581 gttgcattgg aaactatatt ttggtaagct gctcacataa ggaaatcagg gctattcccc
     2641 tggaggtacc acatggccga tgcgacgata actcccgttt ctccctcaac cgtgcagctg
     2701 acgccccgga cggtcagccg tcgctgggcg ctcggcgacc cacgcccgac gtcgcagtcg
     2761 gaccgggcgc ggatggtgct cgatctcatg gatcccgcgg tcaccgaccc cttcgtcgtc
     2821 ctcgccgagg actggttcag cgaggtcggc ttcgactggc atccacaccg tggcttcgaa
     2881 accgtcacct acgtgatcga tggcgaactc gagcacaagg acagcacggg cgggcacgcc
     2941 gtcctcaccc ccggcgacgt gcaatggacg acgatggggc acgccgtgct tcacgccgaa
     3001 ctcgcctatc agaagaaggg cgtccacacg ctccagctct ggttgaactc gcccgccgcg
     3061 ctgaagatga tcgagccagg ctaccagaac ctctcggcgt ccaccgtgcc gacgatcgag
     3121 cgggacggcg cccgcgtgcg cgtctacgcc gggtccgtgc agggcgtgac cggtcccgcg
     3181 gcgacgatct ggcccgcgac cgtgatcgac gcgaggctct cggccggcgg gcacttcaca
     3241 cacgaactgc catcgacctt cgccggtctc atgtacgtga tcgaggggaa ggttcgcgcc
     3301 gggagtgacg gcaccgtcgt tgcgacgggt gaagcggcct ggttcgacca catcgatctc
     3361 cgggaggatg ggagggcccc ttcggcaacc ggtatcgaga tcgtagccga cgaggagacg
     3421 ctgttgatct tctacgccgc ggcaccgatc ctcgagcccg tcgttgccta cggtcccttc
     3481 gtgatgaata ctcgtgaaga gatcatccag acctacgagg actaccagtc cggaaagttc
     3541 ccggcttacc cgaactggta accggtcaga gcatcgtgcc tttcgtatgc aaggagtacc
     3601 ccacatgaac gtcaacgaag cgattcgcac cgtcctcgcc gtccgcgcct tccaggacaa
     3661 gccggttccg gccgggatcc ggcaggagat actcgaggcc gcgcatctca gcgccagcgg
     3721 ctccaacaag cagccgtggc acttcatcgc gatcgaggat ccggagcgga tcaaagggct
     3781 cgccaggttc acgccgaccg gcccttacgt cgccgaggct ccgttcgtcg tcgtcgtcgc
     3841 ggcgaagaag ggattgaacg gtctctccga cgcgagtcgc gcgatccagg acatgatcct
     3901 cgttgcctgg tcggaaggcg tcggttcgaa ctgggtcggc tggggaaaca cgctcgatgg
     3961 cgtgaaggaa ttcgtcggca tcccgtccga gagcgatctt gagatcatcg cggtccttcc
     4021 gttcggctat ccggtggaga gcgtcggcaa gggcaagaag aatcgcaagc cgttcgacga
     4081 ggtgatccat cgcgagacct tcagcggcgt cgcgtagtca cccgcctcac gtatggcatc
     4141 caagacgggg cccgggatca actgatcccg ggcttcgcca cgtcctccaa tcgc
//
LOCUS       DASTMD010000086         7032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3132982, whole genome shotgun sequence.
ACCESSION   DASTMD010000086 DASTMD010000000
VERSION     DASTMD010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3132982"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..807)
                     /locus_tag="VFQ54_01310"
     CDS             complement(268..807)
                     /locus_tag="VFQ54_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163647.1"
                     /translation="MTIRQRLAITATMLTLAFASCSPLAAHAATGTPAASPITVPTVT
                     GNGCDQLGSYFKQLAKLTAENDGLTILATVNNDALALTSAKAAEVARSLDDLIAELKA
                     VTPPTAAKTYHLAYIDFITWYRDLALHRDAAAHQRLINADKRIFPALGRGISAGQAIC
                     GADAWNAAQQAAFPPTPTP"
     gene            1012..2508
                     /locus_tag="VFQ54_01315"
     CDS             1012..2508
                     /locus_tag="VFQ54_01315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0163648.1"
                     /translation="MSAAAGTTDRLRIDGWRGKALLPVLIYSALVMSVLSTLGSPMIP
                     TIARDQGVSLDAAQWILTVTLLIGAVSTPITGRLADGPYRKLTILGSLILVLIGSILA
                     ATSPNFAVLITGRALQGLGLGLVPLAISVARDCLPADKVRGGIAILSITTAAGAGLGY
                     PITGLIAQEFDYRAGFWFAAIISVIAIALVATVVPTGSLRASHPLDLIGSVLLGTTLT
                     ALLLAISQGEVWGWGSATIVGLFIATIVLGAIWVWQALRTTHPLVDLRLMTNRSVLTA
                     DLVSLLMGVSLYAMSALINRYVQTPKGAGYGFHAGLIATGLMLAPLSIGSLLSTRTSR
                     FLTGRFGPAQVLPIGSIIVGLDMLFIALERTSRWEIVVAMILLGLGIGTTFAAMPLLI
                     IRAVPASETGSATSMNTVLRSVGGAIGSAASIALLTSYTPAGDVLPTDNGYTVTFVVG
                     AVICLASAVVSVIMLPRTPVSGAGLQAPVLERIPATPVVEPQAQAQRG"
     gene            2530..3162
                     /locus_tag="VFQ54_01320"
     CDS             2530..3162
                     /locus_tag="VFQ54_01320"
                     /inference="COORDINATES: protein motif:HMM:NF037150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEU0163649.1"
                     /translation="MATTAETDGISAGTEVGGGGVRKRDASATRQALLEAANELFGTK
                     GYDHTSLREIGERAGVDAALIARYFGGKVQLYSAALRADAETTEGTEDRSTPELILRR
                     LIRRADNHGLGPIIRALVAAEVDPDLRQTAREHISQRLLEPLTERIAGLGVPDPELRA
                     ELIVAAIVGVITIRASGAFPTIAVADPDEIVAVIGPMLEGIATAAPGDRV"
     gene            3159..4550
                     /locus_tag="VFQ54_01325"
     CDS             3159..4550
                     /locus_tag="VFQ54_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0163650.1"
                     /translation="MSEGVTTAGSRAVRIISVAPFFSTEWPSGVFAFGNAAAADLTLA
                     HVRVEVEDRAGRTAEGWGAIFLDHAWAFPGDDLSDEAKDQIMRAIIGMAGNRLIADGD
                     YGHPLDHFLAIEPQLGEIAIGRGGGGTIDVPLLAALVACSPLDAAIHDAYGKLHGVSS
                     YDALGPDFVAWDLGRVLGERFAGRYLPDFLRSQPVDFLPIMHAVGMSDPLTPAEQGTS
                     AYHCLTDWIAIDGVHCFKVELRGKDLAWDIARLVDVYTTASESVGLAGEVRIFADLDE
                     QGSSPEYVRSLLDGVETRSAAAFAALDAIQQPVRRDPGAGAADFRDVSSRVPVVLDEG
                     LTSLAAIDHAVALGWNGIVLETGKTQSLVLLALAKASELGLHVSVQDLTNPGIALLQS
                     VGLAARLPRIMPVENNQRQYFPATSKPEERVFPWIFAVRDGGVPANELTGPGLGYGDL
                     ARIDRDIFSAPPV"
     gene            4582..5841
                     /locus_tag="VFQ54_01330"
     CDS             4582..5841
                     /locus_tag="VFQ54_01330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0163651.1"
                     /translation="MVSATATTSATTRELSPARRWSMLGLGMGAQAAGTVFVNAPAFL
                     IPTLHDERGLSLARAGLLVGMPTLGMMLTLILWGALVDRVGERWVLAGGLLLTALAGL
                     GAVMAGSLVATGAFLLLGGMAAASANAASGRVVVGWFPPEKRGFAMGIRQMAQPIGVG
                     VAALTIPLLAAHEGLNTALIVPTILTAVVGVVCAIAVIDPPRPSRKQADSSQLANPYR
                     QSDLLWRIHGVSILLVIPQFTVWNFALVWLISRHGMSETTAGLVIVVSQILGAIGRMA
                     VGILSDRIGSRMRPLRTISVAAICSMLLLGLTDWTGSWIAVVVMLVASVITVADNGLA
                     FTSVAEIGGPFWGGRAMGMQNTGQFLAAAVVPSVIGGLIGWVGYPLTFALVSIFPAIA
                     TPLVPKVDRIEPPAAPVLVPGPEPKVA"
     gene            6298..6828
                     /locus_tag="VFQ54_01335"
     CDS             6298..6828
                     /locus_tag="VFQ54_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163652.1"
                     /translation="MNLERELGAPAEQVVPQSHEQKRRDFVLQIIQPGLAGMMDGSVS
                     TLAPIFAAAFAVSNPHTVFLIGLAAALGAGVSMAFAEALSDDGSLTGRGSPWLRGGVE
                     GAMTFVGGFGHTLPFLLPHMGMALTLAYVVVAIELVIISWIRFHYFRMNFVRSSLQVL
                     GGGLIVVIIGVVLGQS"
BASE COUNT         1043 a         2229 c         2414 g         1346 t
ORIGIN      
        1 cgtgctcgtg ccatcggcgc gtcccgggaa gcggatcggc tgcgccgacc cgtgtccccg
       61 ggaacgtcgt gcaccgcatg ttcccgttgg cgtccgcctg cggcggatgt gcttcgcacg
      121 gggatttctc gacaggctcg aaatgacgaa tgtgcacgcg acagcgtagg gcatgccgcc
      181 ctaccaaccc ccgcactgcc agtgatgtcg acggttctgg attcttttcc tacgccggcc
      241 tctatggctt ctatctgcac cggcgcccta tggcgtcgga gtcggcggga aggcggcctg
      301 ttgcgccgcg ttccaggcgt cggcgccgca gatcgcctgg ccggcactga tgccgcgccc
      361 gagcgccggg aagatccgct tgtcggcgtt gatcagccgc tggtgcgcgg cggcgtcgcg
      421 atgaagcgcg agatcgcggt accaggtgat gaagtcgatg taggcgaggt gataggtctt
      481 cgcggcggtc ggcggcgtca cggccttcag ctcggcgatg aggtcgtcga gtgatctcgc
      541 gacctccgcc gccttggcgc tggtcagggc gagggcgtcg ttgttcacgg tggcgaggat
      601 ggtcaggcca tcattctccg ccgtgagctt cgccagctgt ttgaagtagg acccgagctg
      661 gtcgcaaccg tttcccgtca ccgtcggcac cgtgattgga ctggcggcgg gtgtgcccgt
      721 ggcggcatgc gcggcgagcg gggagcatga cgcgaatgcg agcgtcaaca tcgtggcggt
      781 gatcgcgagg cgctggcgga tagtcatcgt ttttcctcat ggtcgcgcgc atggacgttt
      841 ctcacagtat cgcaagcggg gcgcttgtgc gcagtacctg tgagcatccc cacggtctgt
      901 aaattgcctg tgagaaatac cttgtcattg tgtgatgtca tggtatgatg acttatggat
      961 ttgacaccgg ttgggtgcca tggatacggt cacgacaggt acgagaagac gatgagcgcg
     1021 gcggcaggaa cgacagaccg attgcgaatt gatgggtggc ggggaaaggc gctgctgccg
     1081 gtgctgatct attccgccct ggtgatgtcg gtgctctcga ccctcggctc gccgatgatc
     1141 ccgacgatcg ctcgcgacca gggcgtctcg ctcgatgcgg cgcagtggat cctcacggtg
     1201 acgctgctga tcggcgcggt ctcgaccccg atcacgggcc gcctggcgga cggaccgtac
     1261 cgaaaactga cgatcctcgg gagcctgatc ctcgtgctga tcgggtcgat tctcgccgcg
     1321 acgtctccca atttcgcggt cctgatcacc ggtcgcgcat tgcagggtct tgggctcggc
     1381 ctcgtgccgc tcgcgatctc ggtcgcgcgt gattgcctcc ccgccgacaa ggtgcgcggt
     1441 ggcatcgcga tcctctcgat cacgacggcc gccggcgccg gtctcggata cccgattacc
     1501 ggtctcatcg cgcaggaatt cgactaccgt gccggattct ggttcgcggc gatcatcagc
     1561 gtgatcgcga tcgcactcgt cgcgacggtg gtcccgacgg gatcgctgcg ggcgagccat
     1621 ccgctcgatc tcatcggcag cgtcctgctc ggcacgacgc tgaccgcgct gcttctcgcg
     1681 atcagccagg gcgaggtgtg gggatgggga tcggcgacga tcgtcgggct cttcatcgcg
     1741 acgatcgtcc tcggcgcgat ctgggtctgg caggcattgc ggacgacgca tccgctcgtc
     1801 gatctccgcc tgatgacgaa tcgctcggtg ttgaccgcgg atctcgtctc cctgctgatg
     1861 ggagtcagcc tctacgcgat gtcggcgctg atcaaccgct acgtccagac accgaagggc
     1921 gccggctacg gttttcacgc cggcctgatc gccaccggat tgatgctggc gccactctcg
     1981 atcggcagtc tcctctcgac gcgaacctcc cgcttcctga ccgggaggtt cggacccgcc
     2041 caggtattgc cgatcggctc gatcatcgtc gggctcgaca tgctcttcat cgcgctcgag
     2101 cgaaccagcc gctgggaaat cgtcgtggcg atgatcctgc tcggtctcgg tatcgggacg
     2161 accttcgcgg cgatgccgct cctgatcatc cgcgcggtgc cggcatccga gacgggaagc
     2221 gcgaccagca tgaacaccgt gctgcgctcg gtcggtggcg cgatcggcag cgcggcgagc
     2281 atcgccctcc tcacgagtta cacgccggcg ggtgatgtcc ttccaaccga taacggatac
     2341 acggtaacct tcgtggttgg ggcggtgatc tgcctcgcct cggcggtggt cagtgtcatc
     2401 atgttgccac gcaccccggt ttccggggcg gggctgcaag cgccggtcct ggagcggatt
     2461 cccgcgacac cggtggtcga gccgcaagca caggcgcagc gcgggtaggt cctgaggaag
     2521 gaaatgacga tggccacgac agccgagaca gacggcatca gcgcgggcac ggaggtcgga
     2581 ggcggtgggg tgcgcaagcg cgacgccagt gccacgcgcc aggcgctgct cgaggcggcc
     2641 aacgaactct tcgggacgaa gggctacgat cacacctcgc tccgcgagat cggcgaacgc
     2701 gccggtgtcg atgccgcgtt gatcgcccgc tacttcggtg gcaaggtgca actttactcg
     2761 gcggcgctgc gggctgatgc cgagacgaca gaagggaccg aagatcgctc gacgccagag
     2821 ctgatcctgc ggcggttgat ccggcgggcc gacaatcacg gcctgggacc gatcatccgg
     2881 gcgctcgtcg cggccgaggt ggatcccgat ctccggcaga ccgcccggga gcacatctcg
     2941 cagcgccttc tcgagccgtt gacggagcgc atcgcgggac tgggagttcc ggatccggaa
     3001 ctccgcgcgg agctgatcgt cgcggcgatc gtcggtgtga tcacgattcg ggcatccggg
     3061 gcctttccca ccatcgccgt cgccgatcca gacgagatcg tcgccgtgat cggtcccatg
     3121 ctggagggca tcgcgaccgc cgcgccggga gatcgcgtat gagcgagggt gtcaccacgg
     3181 ctggttcacg ggcggtgcgg atcatatccg tagcgccctt cttctcgacg gaatggcctt
     3241 ccggcgtctt cgccttcggg aatgcggctg cggctgatct cacgctcgcg cacgtgcggg
     3301 tcgaggtcga ggatcgcgcc ggtcggaccg cggaaggatg gggcgcgatc tttctcgacc
     3361 acgcctgggc atttcctggg gacgatctga gcgacgaggc gaaggaccag atcatgcgcg
     3421 cgatcatcgg catggccgga aatcgcctga tcgcggatgg cgactacggt catccgctcg
     3481 atcactttct cgcgatcgag ccgcagctcg gggagatcgc gatcgggcgg ggcggcggtg
     3541 gcacgatcga cgtcccgctt ctcgccgcgc tggtcgcctg ttccccgctc gatgccgcca
     3601 ttcacgacgc ctacggcaag ctccacggcg tcagttcgta cgatgccctc ggtccggact
     3661 tcgtcgcgtg ggacctggga cgagtgctcg gcgagcgttt cgccggtcgc tacctgccgg
     3721 atttcctgcg atcgcagccc gtcgacttcc tgccgatcat gcatgcggtc ggcatgtccg
     3781 atcccctgac cccggcagaa cagggaacca gtgcctacca ctgcctgacc gactggatcg
     3841 cgatcgacgg tgtccactgc ttcaaggtcg aactcagggg caaggatctc gcctgggaca
     3901 tcgcgcggct cgtcgatgtc tacacgaccg cgagcgaaag cgttgggctc gccggcgagg
     3961 tgcggatatt cgccgatctc gacgagcagg gatcgtcgcc ggaatacgtt cgatcactgc
     4021 tcgacggtgt ggagacgcgt tcggcagcgg cgttcgcggc gctcgacgcc atccagcagc
     4081 cggtgcgtcg ggatcctggt gcgggtgcgg ctgatttccg ggatgtctca tcccgggttc
     4141 ccgtcgtact cgacgagggg ctgacgagtc tcgccgcgat cgatcacgcc gtggcgcttg
     4201 gctggaacgg gatcgtgctg gagactggca agacgcagag cctggtgttg ctcgcactgg
     4261 cgaaggcgag cgagcttggc ctgcatgtct ccgtgcagga cctgacgaat cccggcatcg
     4321 cgctccttca atcggttggt ctcgcggcgc ggttgccgcg gatcatgccg gtagagaaca
     4381 accagcgaca gtatttcccg gcgacatcga agccggagga gagggtcttc ccctggatct
     4441 tcgccgtgcg ggacggtggt gtcccggcca atgagctaac cggtcccgga ctcgggtacg
     4501 gcgatctcgc tcgcatcgat cgcgatatct tctccgcccc gccggtctag acgtttccgg
     4561 tacgacccga gagagcacga catggtatcg gctacggcca ccacatctgc cacgacgaga
     4621 gaactgagtc ccgcacgacg ctggtcgatg cttggactgg gaatgggcgc gcaggccgcg
     4681 gggaccgtct tcgtcaatgc gccggccttt ctgatcccga ccctgcatga cgagcgcgga
     4741 ctgagtctcg cccgcgccgg cctcctcgtc gggatgccga cactcggcat gatgctgacg
     4801 cttatcctct ggggcgcgct cgtcgatcgc gtcggcgagc ggtgggtgct cgcgggtgga
     4861 ctcctcctca cggcactggc cgggttgggg gcggtgatgg cggggtcgct ggtcgcgacc
     4921 ggcgcgttcc tcctgctcgg cggaatggcg gcggcgagcg cgaacgccgc gagcggacgc
     4981 gtcgtcgtcg gctggttccc accggagaag cgcggtttcg cgatgggcat ccgccagatg
     5041 gcgcaaccga tcggcgtcgg cgtcgccgcg ctgacgatcc ccttgctcgc cgctcacgag
     5101 gggctcaata ccgcgctgat cgtcccgacg atcctcacgg cggtggtcgg cgtcgtctgt
     5161 gcgatcgcgg tgatcgatcc gccgcgacca tcgcggaagc aggcggacag cagccagctg
     5221 gcgaatccgt atcgccagag cgatctgttg tggcgaattc acggggtctc gatcctgctc
     5281 gtgattcccc agttcacggt ctggaacttc gcgctcgtct ggctgatctc gcgccacggg
     5341 atgtcggaga cgacggccgg tctggtgatc gtggtctcgc agatcctcgg tgcgatcggt
     5401 cgcatggcgg tcggcatcct ctccgacagg atcggaagcc ggatgcgccc gctccgcacc
     5461 atctcggtcg ccgcgatctg ttcgatgctc ctgctcggtc ttaccgactg gacgggctcc
     5521 tggatcgcgg tcgtggtgat gctcgtcgcc tcggtgatca cagtggccga taacggactc
     5581 gcgttcacct cggtcgcgga gatcggaggc ccgttctggg gtggccgggc gatggggatg
     5641 cagaacaccg ggcagttcct ggcggcggcg gtcgtgccgt cggtgatcgg tggactgatc
     5701 ggctgggttg gttacccgtt gacgttcgcg ctggtctcga tcttcccggc gatcgcgaca
     5761 ccgctcgtgc cgaaggtgga ccggatcgaa ccacctgccg ctccggtgct cgtccccggt
     5821 cccgagccga aggtcgcata gtcggccccg gtcctgcctc accgtatcgt ccaggccagg
     5881 cgcatcgtgc gggaggcatg tggacgccgg ctgatccctc gacaggctcg ggaggacgtg
     5941 tcttgtgcgg cgtcgtcggg ggaggcatgt gctgattccc gttcccacgg cgcttgtcgc
     6001 cgttcccgga aggcgtcgtg cgggaagcag gtgggaccca gctgatccct ctacaagctc
     6061 gggaggacgg ttggtggctc gggaggacgt gtcttgtgcg gcgtcgtggg ggaggcatgt
     6121 gctcattccc gtccccacgg cgcttgtcgc cgccgttccc ggagggcgtc gtgcgggagg
     6181 cgcggggact ccgacccggg aggtcgtata tgagaatgat tattgtttag aaatgagtct
     6241 cgatgtagac tggtgcaacg ggcgtcgcct cggggcgccg gctacaggag attcacgatg
     6301 aatttggaac gcgaactcgg cgccccggca gaacaggtgg tgcctcaatc ccacgaacag
     6361 aaacggcgcg acttcgttct ccagatcatc cagcccgggc tcgccgggat gatggatggt
     6421 tccgtctcga cactcgcccc gatcttcgcg gcggccttcg ccgtcagcaa tccgcatacc
     6481 gtcttcctga tcggtctcgc cgccgcgctc ggcgccggcg tctcgatggc cttcgccgag
     6541 gcgctctccg acgacggctc gctcaccgga cgtggttcgc cgtggcttcg cggcggcgtc
     6601 gagggcgcga tgaccttcgt cggtggcttc gggcacacat tgccgttcct gcttccccac
     6661 atggggatgg ccctcacgct cgcctacgtc gtcgtcgcga tcgagctggt catcatctcg
     6721 tggatccgct tccactactt ccggatgaat ttcgtccgct cgtcgcttca ggtgctgggc
     6781 ggcggtctca tcgtcgtcat cattggggtg gtcctcggtc agtcctgatg gaggatgcat
     6841 tcaacggaga agggccggtg gcaatcacgc caccggccct tcttgtgttg aatcagaccc
     6901 agacgtatag agcggctgcg aaaagatgtg cctgcggaag tgctcgacgg ggcatggcgt
     6961 gccggacgaa cgcgttggag tccggctgcg ccggatgcgc ttcgcgcggg gatccttcga
     7021 caagctcagg at
//
LOCUS       DASTMD010000087         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1079011, whole genome shotgun sequence.
ACCESSION   DASTMD010000087 DASTMD010000000
VERSION     DASTMD010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079011"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VFQ54_01340"
     CDS             <1..380
                     /locus_tag="VFQ54_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU0163653.1"
                     /translation="VTPVLTIPTIELARRYSIPTVIGSFTPTEILTAWQAGATFVKVF
                     PASAVGPGYLKDVRGPLPQVKLIPTGGVTLQNAGDFIRAGASAIAVGSNLVDAKTVKA
                     GDWKTLAERGAAFIDAVKSARAS"
     gene            493..780
                     /locus_tag="VFQ54_01345"
     CDS             493..780
                     /locus_tag="VFQ54_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163654.1"
                     /translation="MPILRRLVEQAMADERFRAVARGDLDEALRSNGYTLNDRERTLV
                     FRFRDALNEAGIDVLLAHTGGQDLGSFLDDMDPGDLEALVRIAPKPGATPD"
     gene            complement(819..1469)
                     /gene="upp"
                     /locus_tag="VFQ54_01350"
     CDS             complement(819..1469)
                     /gene="upp"
                     /locus_tag="VFQ54_01350"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014471148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU0163655.1"
                     /translation="MNDIVRPPSLHVSMHPLVRHKRTLLSDKQTDSKTFRELVRELTR
                     LVLYEATADIPTDSVTYSTPMEETVGDEISIKIGLVPILRAGLGMVDAAVDALPSAEV
                     WHLGMYRDEKTHEPVSYYNRLPSRCPDDLIIVLDPMLATGGSAVDAVNVLKQWGATWI
                     KFAGIIAAPEGARALHAAHPDVDIFVAALDRELDDDRFIRPGLGDAGDRLFGTHDT"
     gene            complement(1499..>2091)
                     /locus_tag="VFQ54_01355"
     CDS             complement(1499..>2091)
                     /locus_tag="VFQ54_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0163656.1"
                     /translation="GDWAAGDRGIAAQPDRREEFGVGVHQAVRYALTLHPPRINCLAG
                     KMNDDDDRSDAVLISNVNFAAEALKALDVQLVVEPVNDKDVPTFGIPTTRAALELIAE
                     VESDNVGLQYDVYHSMMMGEDPFAIIPDLAGQISHLQIADVPGRHQPGTGAIDFARLL
                     EVIDDSGYAGWTSLEYNPEGATADGFGLLRDMGLLG"
BASE COUNT          357 a          651 c          706 g          377 t
ORIGIN      
        1 tcgtgactcc cgttctcacg attcccacga tcgagctcgc ccgccgctac agcatcccga
       61 cggtgatcgg gtcattcacg ccgaccgaga tcctgaccgc ctggcaggcc ggagcgacct
      121 tcgtcaaggt cttccccgcg agcgccgttg gtcccggcta tctcaaggac gtgcgtggtc
      181 cgctcccgca ggtgaagctg attcccacgg gaggagtgac gctccagaac gcgggtgact
      241 tcatccgggc gggagcgtcg gcgatcgcgg tgggaagcaa cctcgtcgac gcgaagacgg
      301 tgaaggccgg tgactggaag acgctcgccg agcggggagc cgccttcatc gatgcggtga
      361 agagcgcgcg ggcatcgtga ggacgtacaa tcagcgacac caaccttggt gtcgaagcgg
      421 cgaagaggag ggcttccggc agagcggagg ctcgtccgtg agtagtgttc aatgagtcaa
      481 gatccaattc gcatgcccat cctcaggcgt ctggtcgagc aggcgatggc ggatgagcgt
      541 ttccgggccg tcgctcgcgg tgatctcgac gaggcgctca ggagcaacgg ctacacgctc
      601 aacgatcgcg agcggacgct ggtctttcgc tttcgcgatg cgctgaatga ggccgggatc
      661 gatgtcctgc tcgcccacac gggcggccag gatctcggct cgttcctcga tgacatggat
      721 cccggggacc tcgaggcgct ggtccgcatc gcgccgaagc cgggcgccac tccggactga
      781 gcgcggacgc ccggctggcg gatttgcgag cggggatgtc aggtgtcgtg cgtgccgaag
      841 aggcgatcgc cggcatcgcc gagaccgggg cggatgaacc ggtcgtcgtc gagttcgcga
      901 tcgagcgccg cgacgaagat gtcaacgtcg gggtgcgccg catggagcgc ccgcgcaccc
      961 tcgggggcgg cgatgatccc ggcgaacttg atccacgttg ccccccattg cttcaggaca
     1021 ttcaccgcgt cgaccgccga gccacccgtg gcgagcatgg gatcgagcac gatgatcagg
     1081 tcgtccgggc agcggctcgg gagccggttg tagtagctca cgggttcgtg cgtcttctcg
     1141 tcccggtaca ttccgagatg ccagacttcg gcgctgggaa gcgcgtcgac cgcggcgtcg
     1201 accatgccaa gaccggcgcg caggatcggc accagcccga tcttgatcga gatctcgtcg
     1261 ccaaccgtct cttccatcgg ggtcgaatag gtgacggaat cggtggggat gtcggccgtc
     1321 gcctcgtaga ggacgagacg ggtcagttcc cggacgagtt cgcgaaaggt cttgctgtcg
     1381 gtctgcttgt ccgagagcag ggtgcgcttg tgtcgcacca gcggatgcat gctgacgtga
     1441 agcgaggggg gacgaacgat gtcgttcatg gacttctccg cgtgttgttg gcgatcgatc
     1501 agccgagcag ccccatgtcg cggagcagtc cgaatccgtc cgcggtcgcc ccttccgggt
     1561 tgtattcgag acttgtccat cccgcgtagc ccgaatcgtc gatcacctct aagagtcgcg
     1621 cgaagtcgat cgcaccggtt cctggctgat ggcgacccgg aacgtcggcg atctggaggt
     1681 gcgagatctg gccggcgaga tccgggatga tcgcaaacgg gtcttcaccc atcatcatcg
     1741 agtggtagac atcgtactga agaccgacgt tgtcgctctc gacctcggcg atcagctcca
     1801 gtgccgcgcg agtggtcggg atgccgaacg tcggaacatc cttgtcattc acgggctcga
     1861 ccacgagctg gacgtcgagc gccttcagcg cctccgcggc gaagttgacg ttcgagatca
     1921 ggaccgcatc gctgcgatca tcgtcgtcat tcatcttgcc ggcgaggcaa ttgatccgcg
     1981 ggggatgcaa ggtcagtgca tacctgaccg cctgatgcac gcccacgcca aactcctcgc
     2041 gacggtctgg ctgggcggcg atgccacggt cccctgccgc ccagtcccct g
//
LOCUS       DASTMD010000088         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1971984, whole genome shotgun sequence.
ACCESSION   DASTMD010000088 DASTMD010000000
VERSION     DASTMD010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971984"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..436)
                     /locus_tag="VFQ54_01360"
     CDS             complement(185..436)
                     /locus_tag="VFQ54_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163657.1"
                     /translation="MFSLWDERSLDPVGMYRTLDDALAVVREGVELNGPGDTISLSLV
                     EDLPGGELRQIANGARLAAMANAAGAVKSKPPARSSVAD"
     gene            complement(973..1566)
                     /locus_tag="VFQ54_01365"
     CDS             complement(973..1566)
                     /locus_tag="VFQ54_01365"
                     /inference="COORDINATES: protein motif:HMM:NF021453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2165 domain-containing protein"
                     /protein_id="HEU0163658.1"
                     /translation="MSRSNRAVAGDADPLPRWLQLGSLPVVAAILVALNGLYILFVAF
                     GNITDYGTNQPFVEHVLSMDTTNFGAGEGQDLDSDVMWRAIDNRTLQNAAYVLIIVWE
                     SLTAIVLLGSLFYWVTERGRGYRIARAASSMGLVMLLLLFMGGFIAIGGEWFQMWKSS
                     DWNGLDSAFRVAVLAFISLVLIHIPSSHWNDMGPGSS"
     gene            1920..2531
                     /locus_tag="VFQ54_01370"
     CDS             1920..2531
                     /locus_tag="VFQ54_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0163659.1"
                     /translation="MHNAILDAARLIVDEKGVDKLTLRGIAQHLGYSAGAIYEYFDSK
                     EAIIEALYFTSTGGLGQYMIETMAAVPPETDAVERMRTLAFAYREFAHRNAELYWLTF
                     SAMKQRPERLLRGHDEGLFSGFEGLVAVIEQGVADGLLIDTIPPLEIAFTAWSAVHGF
                     VSLELSGHLKDHPATGEPLAADSEDHFFDQLIEILFHGFVRHR"
     gene            2622..>3430
                     /locus_tag="VFQ54_01375"
     CDS             2622..>3430
                     /locus_tag="VFQ54_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU0163660.1"
                     /translation="MFASWGRFVAFHRRPVAIVALLSIILSVAVMVTVAPDLSSEGFL
                     SDNAESSRVDDTVSQQFGQGGDTLIFLFDTTGKMTDPGMQAKVETALAPVTQSGDFRQ
                     IVTTWSTGNSALISNDGASTYAIAYEKPGYSVSNGKADSLTSQVEDAAKSQGLTVETG
                     GSLAVGSAISSQVEKGIVRAESVAIPLTIIMLLLVFGTLVAAGLPLLIGVLAILLSIG
                     GLFVLSQEAFSSVFAINVITMLGLGLGIDYSLFMVTRFREEIARRSPEEAI"
BASE COUNT          682 a         1106 c          986 g          656 t
ORIGIN      
        1 ccacgctcga tgcaacctgg ggttccgccg aggactacct gcgcaaggtg ggcctgacca
       61 atgcccagct cgattcgctg cgcacgaacc tcctcgagcc ggcgtcgtag tcgctcagtc
      121 gagttgaacc acaaggaaga ggacgggacg gtctctggag accgtcccgt cctcttcctc
      181 tggctcagtc ggcgacactc gaacgcgccg gtggtttcga tttcaccgct ccagcagcgt
      241 tggccatcgc ggcaagcctg gctccattcg ctatctgccg aagttcgcca cctggcagat
      301 cctccacaag ggagagggaa atggtatcgc cgggtccatt cagttccacg ccttcgcgaa
      361 caaccgcaag cgcgtcgtcc agcgtccgat acatgccgac aggatcaagg gagcgctcgt
      421 cccacaggga aaacatgcgc cacctccttt atctacgagg gaacacctga tctaggtgtt
      481 cgacatactt gagcatatca tgacgaatct ctccatgtgc ccaaagggtg tagtgacctt
      541 cagtctcgag gaacgtccga agaaacgtga tcctgtcatc atcatccggg atggaaagag
      601 tcgcgatgaa cagacttcag actggctgac gaccattcct gcgacctgcg atcagacgtt
      661 cagcatggcg aagagctgat tcaagggaat cgaaaaacga aaggcctcga taggcttcct
      721 gcgcctccgg actcctgctt ctcaacggga ttccacgttc ttcgtgtgtc aggaagtcgt
      781 gctccgtggg aggatcctgc tcgacgagtc gatacagcct cgtcgaaatg gctcctccat
      841 cgatttcatg ccacgtggac gtgtttcaca gattcacgga cttctgaccc gatggaattc
      901 tacggcccag gttccctccg gcgcacgtca gtcgggccag accgtaatgc cgggcaacga
      961 ctcgccgcac acctacgatg atccaggacc catgtcattc cagtgcgacg aggggatgtg
     1021 gatcaggacg aggctgatga aggcgaggac cgcgacgcgg aaggccgagt cgagcccgtt
     1081 ccagtccgat gacttccaca tctggaacca ctcgccaccg atcgcgatga aaccacccat
     1141 gaaaagcagg agcagcatca ccagccccat gctcgaggcc gcccgcgcga tcctgtatcc
     1201 gcgtccccgt tccgtcaccc agtagaagag cgatcccaga agcacgatcg cggtcaggct
     1261 ttcccagacg atgatcagga cgtaggcggc attctggagc gtccggttgt cgatcgcacg
     1321 ccacatcaca tcggagtcga gatcctgacc ctcgccggcg ccgaaattgg tcgtgtccat
     1381 cgagaggaca tgctcgacga acggctgatt cgtgccgtaa tccgtgatgt taccgaaggc
     1441 gacgaagagg atgtagaggc cgttcagggc aacgaggatc gcggcgacaa ccggcagcga
     1501 tccgagctgc agccaacggg ggaggggatc ggcatcccct gcaacggcac gattcgaacg
     1561 cgacataaga ccatcctcct cgatacatga acgggtgtgc gcctcgtcgc acggtagcgc
     1621 atcggggatt cgctatccat aggagaatca tccgccgatc ggcccccacc ggaaccgccg
     1681 gcatcccgga tcgggtcggc gtcacgattc cactctcgct ccgctcgcaa ccccgttccg
     1741 ctgcctatcc agaacgcgag aatattctcc gaacatcgtt cagcaaacct attgacattc
     1801 acagatccgc tccctagagt agggacataa caactgaacg tcgttcggaa aggtaatcaa
     1861 tgtccacctc gtctcccgtt tccggtgccg cagcacgtcg agcgcgtaac cacaacgaga
     1921 tgcacaacgc gatcctcgat gcggcccgcc tgatcgtcga tgagaaagga gtcgacaagc
     1981 tcacgctccg cggcatcgcg caacacctcg gatactccgc cggagcgatc tacgaatact
     2041 tcgattcgaa ggaagcgatc atcgaggcgc tctacttcac cagcaccggc gggctcggcc
     2101 agtacatgat cgagacgatg gcggcggtcc ccccggagac ggacgccgtc gaacggatgc
     2161 gaacgctcgc gttcgcctat cgcgaattcg cgcaccgcaa cgccgagctc tactggctga
     2221 ccttttccgc gatgaagcag cgtccggagc gactgcttcg cggtcacgac gagggactct
     2281 tctccggatt cgaaggactc gtcgccgtca tcgagcaggg cgtcgccgac ggactcctga
     2341 tcgacacgat tccgccgctc gagatcgcct tcaccgcctg gtcggccgtt catggtttcg
     2401 tctcgctcga gctcagcggc catctcaagg atcatcccgc caccggtgaa ccactcgccg
     2461 ccgacagtga ggatcatttc ttcgatcagt tgatcgagat cctgttccac gggttcgtcc
     2521 gtcaccgctg atccgcaacc agcgcccgcg gcctggctcg atccacttgc caatctgcct
     2581 atcgcttgcc tgtcgcacgt cagattcagg agaagttcgc catgtttgct tcatggggcc
     2641 gcttcgtggc tttccatcgt cgtcccgtcg ccatcgtcgc cttgctctcg atcatcctct
     2701 ccgtcgccgt gatggtcacc gtcgctcccg atctctcctc ggagggattc ctctccgaca
     2761 acgccgagtc cagccgcgtc gacgacaccg tctcgcagca gttcgggcag ggtggcgaca
     2821 cgctgatctt cctcttcgac acgaccggca agatgaccga ccccggcatg caggcgaagg
     2881 tggagaccgc cctggcgcca gtgacccaga gtggggactt ccgtcagatc gtcacgacct
     2941 ggagcaccgg gaactccgcg ctgatctcga atgacggcgc gtcgacctat gcgatcgcgt
     3001 acgagaagcc ggggtacagc gtgtcgaacg gcaaggccga ttccctcacg tcccaggtcg
     3061 aggatgcggc gaagagccag gggctgacgg tcgagacggg tggctcgctc gccgtcggtt
     3121 cggcgatctc gagccaggtc gagaagggaa tcgtacgtgc cgagtcggtc gcgatcccgc
     3181 tgacgatcat catgttgcta ctcgtcttcg ggacgctcgt ggcggctggg ttgccgctcc
     3241 tgatcggcgt cctcgcgatc ctgctttcga tcggcggtct cttcgtgctc tcgcaggagg
     3301 cattcagttc cgtcttcgcg atcaacgtga tcacgatgct cggtctcggt ctcggcatcg
     3361 actactcgct cttcatggtg acgaggttcc gcgaggagat cgccaggcgg tcgccggagg
     3421 aggcgatcga
//
LOCUS       DASTMD010000089         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_455177, whole genome shotgun sequence.
ACCESSION   DASTMD010000089 DASTMD010000000
VERSION     DASTMD010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455177"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VFQ54_01380"
     CDS             complement(<1..886)
                     /locus_tag="VFQ54_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0163661.1"
                     /translation="MVSPRRRPTQLDVARAAGVSQAVVSYVLNDNQTISIAPETRQRV
                     LDAIATLGYIPNTAARSLRSRTTHTIAAIIPDITNPYYPEFVRGIQDVATAQGYDVLS
                     FNTDGEYEMERQSLDVVRRGRADGVILTAFHLTMDELRPLLEEGTPIALLSDLMDEYL
                     DQPIPLDALAAGGDFAAESLVTYLIERGHTRIGMIAGIESTPPRETRVLGYRHALANH
                     GLTADEIMIRGGDFTEAGGYESMSELLSMSPRPTAVFAANDLMAMGALLACREAGVRV
                     PEDVALAGFDDIPAAKLVH"
     gene            1805..>3098
                     /locus_tag="VFQ54_01385"
     CDS             1805..>3098
                     /locus_tag="VFQ54_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEU0163662.1"
                     /translation="MSQRPHPQPGTATVPHTRTPLIGRRDDLATVCELLRRDDVPLVT
                     LTGPGGVGKSRLALAVVATIGEHFTDGTFFVPLAPVRHAELVIPTIASALGLADMGDA
                     PIADRLTAHLRAQHLLLVLDNLEHVIDIAPDLAEMLARCPRVTILATSRIALHLSDEH
                     EILVAPLDVAEAVQLFTTRAKAASPGFALVVQNAATVAAICTRLDGLPLAIELAAARI
                     RALTPAALLGLLEPSLPLLTDGFRDHPDRLRTMRNAIAWSYDLLTSREQGLFRRLAIF
                     VGGFGLAGVMAVGGDDGIVLSDLISLVNANLVRQEGDLLHDEPRYRMLETTREFAMEL
                     LTLSDENASIHAAFTGHMAKLVDDAASALDGSLQQAWYDRLEAELPNLRSALAWAIAG
                     DADLALQLGGGIHFFWLTRGHLAEARDYLERALTVTGGT"
BASE COUNT          528 a         1037 c          986 g          547 t
ORIGIN      
        1 gatggacgag cttcgccgcg gggatgtcat cgaatcccgc gagcgccacg tcctccggga
       61 cgcggacgcc cgcttcgcgg caggcgagca gcgcgcccat cgccatcagg tcgttcgccg
      121 cgaagaccgc ggtagggcga ggcgacatcg aaagcagctc gctcatcgac tcatacccac
      181 cggcctcggt gaaatcgcca ccccggatca taatctcgtc cgcggtaagc ccgtggttgg
      241 caagcgcgtg ccggtagccg agcacgcggg tctcgcgcgg tggtgtcgat tcgatgccgg
      301 cgatcatgcc gattcgcgtg tgaccgcgct cgatcagata ggtgacgagg gattccgccg
      361 cgaaatcgcc gccggcggcg agggcgtcga gcggtatggg ctggtccagg tactcgtcca
      421 tgaggtcgga gagcagcgcg atcggcgttc cttcctcgag gagcggtcgg agctcgtcca
      481 tcgtgaggtg gaaggcggta aggatcacgc catccgcccg accacgccgg acgacgtcga
      541 gcgattgccg ctccatctcg tactcgccat cggtgttgaa ggagaggaca tcgtagccct
      601 gcgcggtcgc gacatcctgg atgccacgga cgaactcggg atagtagggg ttggtgatgt
      661 ccgggatgat cgccgcgatc gtgtgggtcg tccgcgagcg caggctccgg gcggcggtgt
      721 tcgggatgta gccaagagtg gcgatcgcgt cgagcacgcg ctgacgcgtc tccggcgcga
      781 tcgagatcgt ctggttgtcg ttgagcacgt aggagacgac cgcctgggag actccggcag
      841 cgcgtgcgac atcgagttgg gttggtcggc gacgtggtga gaccaaggtt cggcgtccat
      901 cgggaatggc gacgccagcg gcgccgcggg aaccaatacg tatcagtgat acgtatcatc
      961 ctaccatcgg cccgaaaaga cgcaaccccc ttgtgcagaa tcggctccca cgttcggcag
     1021 cgagcggtga ccatttggca ggacggcact cccgcgagga gaggcgggac atcgaagtcc
     1081 cgccctacga acgcacgctg attccatgcg catttccccg ctgaccacac tggtcgtgac
     1141 ttggcgaatg caccatgtag cggcactgca gcgggtctcg tgtgacccat cgcgcgcagt
     1201 tcccgcgaac cgaacgtccc acacacctct cggaacggcc atctggacgc gatgttcaat
     1261 gcggacgatt cggttcgcgg cggggtatga aaccccgccc taccaatccc gactcggccg
     1321 aaacgccacc atgacgagag ttcgtcgcgc gccggcctcc gggtcaacat gcgttcattc
     1381 ccgtccctac ggcgcttgtc gccgttccgg agacgtcctg cgggccagca gcaggcggga
     1441 cattcctccg ccccacgaac gaccgggctc accagaccag cgcgtggatg cggtgcgcgg
     1501 acggagggcg gaacggtcgt tgccagatcg aagcgctggc ggggaatgtc ctgccgcatc
     1561 ctgccgaacc ccgcagccgc ccgctccctg aatgcgacag gattggaatg tcctcccgct
     1621 agctgactga ccggcggatg ccgtcgcccc acccggccac cggtgctctg gatgccgatc
     1681 gcccgcgcgc gtggggcaaa agcggcacaa atggttgcat tgtgataact catcgatccg
     1741 gtggcgtgat ttaatggaac ctgctgagcc ttcgcatatc cgtcgcgccg gagtcaacgc
     1801 gatcatgtcc cagcgtcccc atccacagcc aggtacggcc actgttccgc acacgcgaac
     1861 gccgcttatc gggcgccgcg acgatctcgc cacggtatgc gagctccttc gccgcgacga
     1921 tgtccccctc gtcacactga ccggaccggg cggtgtcggc aagtcgcgcc tcgccctggc
     1981 cgtcgttgcc acgatcggcg agcacttcac tgacggcaca ttcttcgtcc cgttggcgcc
     2041 ggtgcgccat gcggaactcg tcattcccac cattgcgagc gcactcggac tggccgacat
     2101 gggtgacgcg cccatcgccg acaggctcac cgcccatctg cgagcgcaac acctgctcct
     2161 cgtcctcgac aacctggaac acgtgatcga catcgcaccc gatcttgcgg aaatgctggc
     2221 gcgatgcccc cgcgtgacga tcctcgccac gagcaggatc gctcttcatc tctcggatga
     2281 gcacgagatc ctcgtcgccc cgctcgacgt cgccgaggcg gtgcagctct tcaccacccg
     2341 tgcgaaggcg gcgagtcccg gcttcgccct cgtcgtccag aacgccgcca ccgtcgccgc
     2401 gatctgtacc cgcctcgacg gcctcccgct cgcgatcgaa ctcgccgccg cccgcatccg
     2461 cgccctgacg cctgcggcgt tgctgggatt gctcgagccg tcgctcccgc tccttaccga
     2521 tggctttcgc gatcaccccg atcgactgcg gacgatgcgc aatgcgatcg cgtggagtta
     2581 cgacctcctc acttcgcggg agcagggcct ctttcgccgt cttgcgatct tcgttggcgg
     2641 tttcggactc gcaggagtga tggccgtcgg tggtgacgac ggaatcgttc tttccgatct
     2701 gatctcgctg gtcaacgcaa acctcgtgcg acaggaaggc gatctgctgc atgacgagcc
     2761 gcgatatcgc atgcttgaga cgacacgaga attcgcaatg gaactgctca ccctgagtga
     2821 cgagaatgcg tcgattcatg cggcgtttac cggtcacatg gcaaaactgg tcgatgatgc
     2881 ggcgtcggcg cttgacgggt cgttgcagca ggcctggtac gatcgcctcg aggcggagct
     2941 ccccaacctg cgctccgcgc tggcctgggc aatcgccggg gacgccgatc tggcgctgca
     3001 gctgggaggg ggaatccact tcttctggct gacccgcggt catctcgcgg aagcacggga
     3061 ttaccttgaa cgtgctctca ccgtgaccgg aggaacgt
//
LOCUS       DASTMD010000090         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1616019, whole genome shotgun sequence.
ACCESSION   DASTMD010000090 DASTMD010000000
VERSION     DASTMD010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616019"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VFQ54_01390"
     CDS             complement(<1..767)
                     /locus_tag="VFQ54_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163663.1"
                     /translation="MSAEDTRKVDAGDGDETSSSPDSTEQRSENGAPLQTGVSAPSAG
                     RNIIDTGYGADAIDPAVRKPRIKPPRKAGPQVNPELSGKLRMQGKHPGDRYVKVIRQN
                     KEDFLRIGPGYLVAGEEANAGRGQFGRNFNKLKRVLIGAPLTTASAAHERLTKVKALA
                     VLSSDALSSVAYATEQILAILVLAGASALGVSLPIAGAICVLLAIVGFSYRQTIKAYP
                     HGGGSYIVAKDNLGNLPALVAGGALLTSYTLTVAVSIS"
     gene            1296..>3170
                     /locus_tag="VFQ54_01395"
     CDS             1296..>3170
                     /locus_tag="VFQ54_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163664.1"
                     /translation="MKNATNRKGLAGHFRRLRVKSGSRRPRALGVVSALILVLSLTPA
                     FTTGALAQQSSTTKAKIDVTFLQCSPTSDSADAVQQRSYDDLKTDCTTPDPGVSFHLT
                     NTGNNDDAPKTSDAKGKVTYSALAPGTYTAYTDIPRGSAYEVTWCTADNGTPYQKTFN
                     ESVVTTFEDLQTEQITCSWFVVPTSGATAADAATPQAGVPTLAASDPTSTPATATDLA
                     SISVNLLNCPQASDLTADSGYADLAANCTTRDAGVTLHLTDTSNNNDNPQLSDNAGPI
                     VYPNLQPGTYTAYTDIPRGSATEVVWCSADNGTPYQKTFNQDIVTTFEDLQAEQIVCS
                     WFVIPASASPTTQPATATATATAVPPTVAPTQVPPTATSTPAPSTVAAPTQTPATAAT
                     AAPTTNPGAAQAGTSSIAVNLLLCPQNSSYSSSSSYSDLSSNCTTADPDITVHLTNAG
                     SNVDDPAVSDANGGVTYGNLAAGTYTAYTDIPRATATEVTWCVDNGGTPYQKTFNENI
                     VTTFEDVDSDQVVCSWFVIPTAATPTQTPPPTATVPAGDRTTQTGASVIVHLAACPTD
                     YTGSDLFTDCHGNGIANMEFDLTGPGGTTTENTTVPETPGPGIATFTGLQPGDYTLTG
                     G"
BASE COUNT          597 a         1062 c          967 g          544 t
ORIGIN      
        1 gagatactca ccgccaccgt gagggtgtag gacgtgagca gtgcaccgcc agcgacgaga
       61 gcgggaagat tgccgagatt gtccttcgcg acgatgtagg agccgccacc gtgggggtag
      121 gccttgatcg tctggcggta ggaaaatcca acgatcgcca gcaggacgca gatcgcaccg
      181 gcgatcggaa ggctcacccc gagcgcgctc gcgccggcga gaacgagaat cgcgagaatc
      241 tgttccgtgg cgtaagcgac cgaggagagg gcgtccgacg agaggacggc cagcgccttc
      301 accttggtca ggcgctcgtg cgccgccgaa gccgtggtga ggggagctcc gatcaggacg
      361 cgcttgagct tgttgaagtt gcgcccgaac tgcccacgac cggcattggc ctcttcaccc
      421 gcgacgagat agccgggccc gatgcggagg aagtcttcct tgttctggcg gatgaccttc
      481 acgtagcggt cgccggggtg cttgccttgc atccggagct tgccggagag ttcggggttg
      541 acttgtggtc ccgccttgcg cgggggcttg atcctcggct tgcggacggc cggatcgatc
      601 gcgtcggcgc cgtagccggt atcgatgatg ttccgtcccg cggacggagc cgacaccccg
      661 gtttggaggg gggctccatt ctcgctgcgt tgttcggttg aatcaggtga ggaagacgtc
      721 tcgtcgccgt cgccggcatc gaccttcctc gtgtcctcag cggacatgaa tagcgaactc
      781 cctggaggga ttcacccgaa atcgaggcgg ggatcgtgat gatcgctccg cccaggccgg
      841 gaatccgagt ccttacccca ttttcgatgg tggcgcggta tgaacccgtg ttctccgcga
      901 aggcagttct gcccggacca tcctgacagg acgtgcatga atgcctggaa ggacatccga
      961 cgcggcgaat agtacgatct tcgggagacg atgcacaagc cgcctgcggg tcgacatccg
     1021 gcgttcgagt cacatgtgta cacaagatgt gggccgtgcc gtgtctccca tccccagctg
     1081 cgcccaagaa atgcgcggcg ttgccttcga tcccgcttgc gagcatgatg gaaacgatgt
     1141 tgcaggcaga cgcgttctag ctggtggaag gtaacgtgtc gagtggggcc gcagccccga
     1201 cggttgcgac gcttccatcg ccacgatcca gccggacgca tcactctcga tcgttggggg
     1261 gctggaatcg acaggagtgt ggagaggaaa ctcagatgaa gaacgcgacg aatcggaaag
     1321 gtctggcggg tcatttccgt cgattgcgtg tgaagtccgg aagccgtcga ccgcgggccc
     1381 ttggtgtcgt ttcggcgttg atcctcgtcc tctcgctcac cccggcattc acgacggggg
     1441 cgctcgccca gcaatcatcg accaccaagg cgaagatcga tgtcaccttc ctccagtgct
     1501 cccccaccag cgacagcgcc gacgctgtgc agcagcgctc ctacgatgat ctcaagactg
     1561 actgtacgac cccggatccc ggcgtctcgt ttcatctgac caacaccggc aacaatgacg
     1621 atgccccgaa gacctcggat gccaagggca aggtgaccta cagtgcgctc gctccgggta
     1681 cctacacggc ctacacggac attccgcgtg gttctgccta cgaggtgacc tggtgcaccg
     1741 ccgacaacgg cacgccctat cagaagacct tcaatgaaag tgtcgtgacg acgttcgagg
     1801 atctccagac cgaacagatc acctgctcct ggttcgtcgt gccgacgtcc ggcgccactg
     1861 cggccgatgc cgcgacgccg caggcgggcg tgccgacgct ggccgcatct gatccgacgt
     1921 ctacccccgc gaccgccacc gatctcgcga gcatttccgt caacctgctg aactgcccgc
     1981 aagcctccga tctgaccgcc gacagcggct acgccgatct tgcggcgaac tgcacgaccc
     2041 gggatgccgg cgtcaccctg catttgacgg acacgtcgaa caacaacgac aacccgcagc
     2101 tgagcgacaa cgcgggtccg atcgtctatc cgaatctcca acctggaacc tataccgcgt
     2161 acacggacat cccgcgcggt tccgcgaccg aggtcgtctg gtgctcggcg gataacggga
     2221 cgccctatca gaagaccttc aaccaggaca tcgtgacgac gttcgaggat ctccaggccg
     2281 aacagatcgt ctgctcctgg ttcgtgatcc cggcctccgc ctcgccgacg acgcagccgg
     2341 caaccgcgac ggccaccgcc accgcggtgc cgccgacggt cgctccgacc caggttcctc
     2401 cgaccgccac gtcgacgccg gcaccatcca cggtggctgc gccgacgcag acacctgcca
     2461 ccgcggcgac ggcggctccc acgacgaatc ccggcgccgc ccaggccggg acgtcgagca
     2521 tcgcggtcaa cctgttgctc tgcccgcaga attcctccta ctcgtcgagc agcagctata
     2581 gcgatctctc gagcaactgc accaccgccg atcccgatat caccgttcac ctgaccaacg
     2641 ccggatcgaa tgttgacgat cccgcggttt cagacgcgaa tggcggcgtg acatatggaa
     2701 atctggctgc cggcacctac actgcctaca cggatatccc gcgtgccacg gcgacggaag
     2761 tgacctggtg tgtcgacaac ggtggcacgc catatcagaa gaccttcaac gagaacatcg
     2821 tcaccacctt cgaggatgtc gattccgatc aggtcgtctg ctcctggttc gtgattccga
     2881 ccgccgcgac gccgacccag accccgccgc ccacggcgac ggtcccggct ggcgatcgca
     2941 ccacccagac gggcgcgtcc gtcatcgtgc atctcgcggc ctgcccaacc gattacaccg
     3001 gcagcgatct cttcaccgat tgccatggca acggcatcgc gaacatggag ttcgatctca
     3061 ccggtcccgg cgggacgacg accgagaaca cgaccgtccc ggagacaccg ggtccgggga
     3121 tcgcgacctt caccggtctc cagcccggcg actacacgct gacgggtggg
//
LOCUS       DASTMD010000091         7179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_187578, whole genome shotgun sequence.
ACCESSION   DASTMD010000091 DASTMD010000000
VERSION     DASTMD010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187578"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFQ54_01400"
     CDS             <1..340
                     /locus_tag="VFQ54_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163665.1"
                     /translation="TVSYRRVRLRRATIWYPDASDSREELRRVAAESGMAMLDETTPD
                     GGLIVLLPESGDDGAAVEEDLLKALRAAGYVGQWSTSPARPSSSQSAAIEPSVTPASS
                     QRRPRRPRQS"
     gene            491..2062
                     /gene="gltX"
                     /locus_tag="VFQ54_01405"
     CDS             491..2062
                     /gene="gltX"
                     /locus_tag="VFQ54_01405"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU0163666.1"
                     /translation="MTESTITNDSVVRPAAIPEHLTEVRLRFAPSPTGSLHIGGAKQM
                     MYNILFAHGEAKRAGLKGTVILRIEDTDQKRFVADSVAGIQNGLRWLGFEWDEGPDVG
                     GPYGPYIQSERKHHYKEAAELLVATGHAYPCFCTPERLQQVREERQAKGLPPGYDRHC
                     RNLPADEVRQRLLAGESHVIRFKMPLTGETRFIDLIRGEIVYQNDKIDDLVILKSDGF
                     PTYHLAVVVDDHAMKITHVTRGPEWIPTAPIHVQLYRAFDWDAPQFLHMPLILDPKGG
                     KLSKRNGAVSLDDFRKNGYLPEALVNYLALLGWSLDGETEIFTWQDLLDKFTVERVSA
                     SPSTFDRPKLDWLNQQWINHQISVDDLAVRVLPFLIDAGTIASGPLDDSHPKYAAVRD
                     ASALLKDRIKFLTEAPELMSYFLQDELEAYDPALLIPKKVEPELARKALAALVAIFPE
                     LDVANEEGTEERLRALADELELKPGQLFMPIRVAVTGRTQSPGLFETLKVIGQDRVVA
                     RLRQANDLLDREFTA"
     gene            complement(2052..2777)
                     /locus_tag="VFQ54_01410"
     CDS             complement(2052..2777)
                     /locus_tag="VFQ54_01410"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0163667.1"
                     /translation="MSETAVPANWQTSKRVKTFRKIQDEAIRLFLEKGFDATTIDEIA
                     ETAGVSHMTVFRHFPTKESLVLTDEFDALMEPAIRRRPPEEGPVEALVNVIRDLMASM
                     ADADFDRALARAKLMASTPSLRGGVWANWTMAKEVVTKALLDRANGEADIFQIRVLTA
                     VLIVIAMTASEAWAEQDGQVNLVTLIDRGLATLADEAEILKSLSASAVRVWGQGVYEK
                     SLAHHSEAGQPVCDPERSPMPTR"
     gene            complement(2774..4285)
                     /locus_tag="VFQ54_01415"
     CDS             complement(2774..4285)
                     /locus_tag="VFQ54_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU0163668.1"
                     /translation="MGALALAMLVVGLDATVLNVALPTIAGDLHASTSQLQWVASAYV
                     LALAGAILPAGVLGDRFGRKKLMLVGLGISLLGSIGGALTDSIGPLIAWRTVMGIGAA
                     IITPIVMAMVPLLFNDEDRPKAVGVMTASISLGLPLGPIVGGYLLNHYHWSSIFWINV
                     PFVLLALVAVTLLVQESRDPSAPALDYLGAVLSAAGIVTMVYGFVEAPDKGWVSFQGL
                     GFIVIGLALIAAMIVWQTRTASPLIDLSLFRNTRFVGGIVAMILLTFVLYGLLFTLPS
                     YLQAVRGNDAFGTGLRLLPMMVGLLIAGGGSKRLLTRVGAGTGVAAGTIIVAATMGAL
                     SFLTVDTSMWWIGIALAVFGLGLGMAMTSAMDAVLGALPREKAGSGSGVLNTMRQVSG
                     ALGVAVLGSILSSVYTGKLDDATMASLPAPAAKAVKDSVVGASSVAESMGSQGVSLQH
                     MAGVAYTDAMSVLLIVCAIGGVVAAAISGYILRDRKEMQAASSPTAAQERLQL"
     gene            4522..5010
                     /locus_tag="VFQ54_01420"
     CDS             4522..5010
                     /locus_tag="VFQ54_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163669.1"
                     /translation="MVSSTDEIAKRAYTFGIVAGLRSLTPLALLAFRRNQAPDSAGWK
                     NWPVLSSDRGRKILLAAAAGELIMDKLPNTPSRLKPLSLFGRVTLGSIAGAAIGSEAE
                     GGGEVLPGAIAGAAGGLVGSFGGFLVRKVLTKVFRLPDKLVALSEDGVAIAAAAHVTR
                     LD"
     gene            complement(5007..5453)
                     /locus_tag="VFQ54_01425"
     CDS             complement(5007..5453)
                     /locus_tag="VFQ54_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163670.1"
                     /translation="MSENHDIDVVRTFHEKLGARDISGLLDLATEEIGVGGGRGHGEG
                     KEFFLEWVRYASITVTPRRWFRKVNLEREGRHGMPGELMSTLVVEEDAVWHAARTGRE
                     TGRMRFATIYEVENGRLAGIARYGNIGEAVTTAGMYEENEIAVVAS"
     gene            5599..6795
                     /locus_tag="VFQ54_01430"
     CDS             5599..6795
                     /locus_tag="VFQ54_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0163671.1"
                     /translation="MKITDLKCAVIGDNPIVRIVTDEGISGYGEVESYKPYLAPIVLM
                     LKPALIGEDPTNVERVMRKIRTRGGFKPWGAAVSAVEMALWDVAGKAAGLPVYKLLGG
                     KVRDKVRAYNGAMRTPLAGYTPEDYAASTAAMKARPENFRLIKQAIAFHTPMYAEIPD
                     FFYGEPQQRAFHSHINRGLMTEKGLKHVIACVEAMKEVLGDEVGLALDCGPGWMLPDA
                     IRFAKAVEHLNITWLEDLLTGDYVHWVNADVYRELTTSTSTPIHTGEQIYLRHNFKQL
                     IETHAVNVIGPDPGDVGGIAELKWIAEYADLHGILMAPHGTGNGLLGLAALVQVCATL
                     PDNYVAFETPTGKPEWWFDIVDGLPDPLIVDGTIEVWDRPGMGIELNVEKAAKYLSAG
                     DADFFA"
BASE COUNT         1334 a         2451 c         2200 g         1194 t
ORIGIN      
        1 gacggtcagc tatcgccgcg tgcggctccg gcgcgcgacg atctggtacc cggatgcgtc
       61 cgactccagg gaggaattgc gtcgcgtcgc cgccgagtcc ggaatggcga tgctggacga
      121 gacgactccc gatggcggcc tgatcgtgct gctgccggaa agcggcgatg acggcgcggc
      181 ggtcgaggaa gatctgctca aggcgctgcg cgccgccgga tacgtgggac agtggtcgac
      241 ttccccggcc cggccatcgt catcgcaatc cgccgcgatc gaaccctccg tcacccccgc
      301 ctcgtcccaa cgacgaccgc gacgtcctcg ccaatcctga tttcgcgtca ccatgccgcg
      361 ttatactggg agttcggcct gcggccgtcc ctcccctgca ccagatgcca tatccacgcc
      421 gggagcgggt cgacgagctt gaagcgccag tcgactcctt cgctccgctc tgacatgagg
      481 taccgagacc atgaccgaat ccacgatcac gaacgacagt gtcgtccgtc ccgccgccat
      541 tcccgagcac ctcaccgagg tccgcttgcg gttcgcgccg agcccgaccg ggtcgctcca
      601 catcggcggc gccaagcaga tgatgtacaa catcctcttc gcccacggtg aagcgaagcg
      661 cgcgggcctg aagggcaccg tcatcctccg catcgaggat accgaccaga agcgattcgt
      721 cgccgattcg gtcgccggga tccagaacgg cctgcgctgg ctcggcttcg agtgggacga
      781 gggacctgac gtcggcggcc cctacggccc ctacatccag agcgagcgca agcatcatta
      841 caaggaagcg gcggagctgc tcgtcgcgac cggtcatgcc tatccctgct tctgcacgcc
      901 ggaacgactc cagcaggtcc gcgaggagcg ccaggcgaag ggattgccgc cgggatacga
      961 tcgccattgc cgcaatctgc cggcggatga agtccggcag aggctgctcg ccggcgaatc
     1021 ccacgtcatt cgcttcaaga tgccgctgac cggggagact cgcttcatcg acctcattcg
     1081 gggcgagatt gtctaccaga acgacaagat cgacgatctc gtgatcctga agtcggatgg
     1141 gttcccgacc tatcatctgg cggtcgtcgt cgacgatcac gcgatgaaga tcacccacgt
     1201 cacccgcggt cccgagtgga tcccgacggc gccgatccac gtccagctct accgggcctt
     1261 tgactgggac gcgccgcagt tcctgcacat gccgctgatc ctcgatccca aggggggcaa
     1321 gctctcgaag cgaaacggcg ccgtctccct cgatgatttc cggaagaacg gctacctgcc
     1381 ggaagcgctc gtcaactacc tcgcgctgct cggctggagt ctcgatggcg agacggagat
     1441 cttcacctgg caggatctgc tggacaagtt caccgtcgag cgggtgagcg cctcgccgag
     1501 caccttcgac cggcccaagc tcgactggct gaaccagcag tggatcaacc accagatctc
     1561 ggtcgatgac ctcgccgtcc gcgtcctgcc gttcctgatc gacgccggca cgatcgcgtc
     1621 cggaccgctc gacgattcgc atccgaagta cgcggccgtt cgcgacgcct cggccctgct
     1681 caaggaccgg atcaagttcc tcaccgaggc gccggaactg atgtcctact tcctgcagga
     1741 tgaactcgag gcgtacgacc cggcactgct gatcccgaag aaggtcgagc cggagctggc
     1801 gcgcaaggcg ctggcggcgc tcgtcgcgat cttcccggaa ctcgatgtcg ccaacgaaga
     1861 ggggacggag gagcggctcc gcgcgctcgc ggacgaactc gaactcaagc cgggtcagct
     1921 cttcatgccg atccgcgtcg ccgtcaccgg tcgcacgcag tcaccggggc tcttcgagac
     1981 gctgaaggtc atcggtcagg atcgcgtcgt cgcccgcctc cgccaggcga acgacctgct
     2041 cgaccgcgag ttcaccgcgt aggcatcggg ctccgttcgg ggtcgcacac cggctgtccc
     2101 gcctcggagt ggtgtgcaag cgacttttcg tatacgcctt gcccccagac cctgaccgcc
     2161 gaggcggaga gcgacttcag tatctccgcc tcgtctgcca gcgtcgccag cccgcgatcg
     2221 atcagcgtca cgaggttgac ctggccgtcc tgttccgccc aggcctcgga agcagtcatt
     2281 gcgatcacga tcaggacggc agtcaggacg cggatctgaa agatgtcggc ctcaccgttg
     2341 gcgcgatcga gcagcgcctt ggtcacgacc tccttcgcca tcgtccagtt ggcccagacc
     2401 ccgccgcgca acgacggggt cgacgccatc aactttgccc gggcaagcgc gcggtcgaag
     2461 tccgcgtccg ccatcgacgc catgaggtcc cggatgacgt tcacgagggc ctcgaccggg
     2521 ccttcttccg gcggccgccg ccggatcgcc ggctccatca atgcgtcaaa ctcgtccgta
     2581 agaacgagcg attccttcgt cggaaagtga cgaaagaccg tcatgtggga aacacccgcg
     2641 gtctcggcga tctcgtcgat cgttgtcgcg tcgaagcctt tctccaggaa gaggcggatc
     2701 gcctcatcct ggatcttgcg aaaggtcttg acgcgtttgc tcgtctgcca gttcgccggg
     2761 acggccgtct cactcacaat tgcaaccgct cctgtgcagc agtcggggaa ctcgctgcct
     2821 gcatttcctt ccggtcgcgg aggatgtatc cactgatcgc cgcggcgacg acgccaccga
     2881 tcgcgcagac gatcagcagc accgacatcg cgtcggtata ggcaactcct gccatgtgtt
     2941 gcaatgatac gccctgggag cccatcgact cggcaaccga ggacgcgccg acgaccgaat
     3001 ccttgacggc cttcgccgca ggcgccggca acgaggccat ggtcgcgtca tccagcttcc
     3061 cggtatagac cgaggagagg atgcttccaa gcaccgcgac cccaagcgcc ccggagacct
     3121 gccgcatcgt gttcagcacg ccggaaccgg aacccgcctt ctcccgcgga agggcgccga
     3181 gaacggcgtc catcgccgac gtcatcgcca tccccagtcc aaggccgaag accgcgagcg
     3241 cgatcccgat ccaccacatg ctggtgtcga cggtcaggaa cgagagcgcg cccatcgtcg
     3301 cggcaacgat gatcgtgccc gccgcgacac ccgttcccgc gccaacccgg gtcaggaggc
     3361 gcttgcttcc cccaccggcg atcagcaatc ccaccatcat cggcaacagg cggagcccgg
     3421 ttccgaacgc atcattgccg cgcaccgcct gcaggtagga agggagtgtg aagagcaagc
     3481 cgtagagcac gaatgtcagc aggatcatcg cgacgatgcc accgacaaag cgcgtattcc
     3541 ggaagagact gagatcgatc aacggcgacg ccgtgcgggt ctgccacacg atcatcgcgg
     3601 caatgagcgc gagaccgatc acgatgaaac cgagcccctg gaacgatacc caccccttgt
     3661 ccggcgcttc gacgaatccg tagaccatcg tcacgatgcc cgccgcggag aggaccgccc
     3721 cgaggtaatc gagcgcgggc gccgacggat cccgggattc ctggacgagc agcgtcaccg
     3781 cgacgagcgc gaggagcacg aacgggacgt tgatccagaa gatcgaactc cagtgatagt
     3841 gattcagcag gtaaccgccg acgatcggtc caagcggtag accgagactg atcgaggccg
     3901 tcatcacgcc gaccgccttc gggcggtcct catcattgaa caggagcggc accatcgcca
     3961 tcacgatcgg cgtgatgatc gccgccccga tccccatcac ggtccgccat gcgatgaggg
     4021 gaccgatcga atccgtcaac gcgccgccga tcgacccgag gagagagatc ccgagaccga
     4081 ccagcatcag cttcttgcga ccgaagcgat cgcccaggac accggccggc aggattgcgc
     4141 ccgccaacgc cagcacatag gcgctggcaa cccactgcag ctgactcgta cttgcgtgga
     4201 gatccccggc gatcgtcggc aacgccacgt tcagaacggt agcgtccagt ccaaccacca
     4261 gcattgcgag cgccaatgcg cccaacgcca accaactcga tcttgcggtc atgatttgcg
     4321 taccccgtct gttagtcact aacattccac cagagacatg ttagtgacta acattctacc
     4381 tgtcaagtcc tcgcctgagc gcagagctgc acgtttcgcg gtcgccggaa tacgcgtagg
     4441 atggtcgcgc cgcgtaccat tgcgcgagat cgtcgccctg cgctgacgaa tgtggattcg
     4501 tatcgatgaa gaggagatca gatggtgagt tcgaccgacg agatcgccaa gcgcgcatac
     4561 acgttcggga tcgtcgcggg attgcgttcg ctgacgccgc ttgccctgtt ggcgttccgg
     4621 cgcaaccagg cgcccgactc ggccgggtgg aagaactggc cggtcctgag cagtgaccgt
     4681 gggcgcaaga tcctgctggc cgccgctgcc ggcgagctca tcatggacaa gctgccgaac
     4741 acgccgtcac gcctcaagcc gctgtcgctc ttcggtcgcg tcacgctcgg cagcatcgcc
     4801 ggcgccgcga tcggcagcga ggctgaggga ggcggcgaag tgcttccggg cgcgatcgcc
     4861 ggggccgcgg ggggactcgt cggatcgttc gggggcttcc tcgtgcgcaa ggtcctcacg
     4921 aaggtcttcc ggctcccgga caagctggtc gcgctgagcg aggacggcgt cgcgatcgcg
     4981 gccgccgccc acgtcaccag gctcgactag gacgcgacga cggcgatctc gttttcctca
     5041 tacatgccgg ccgtcgtgac cgcctcgccg atattgccat accgggcaat gccggcgagg
     5101 cgtccgtttt cgacctcgta gatcgtcgcg aaccgcattc ggcccgtctc ccgccccgtg
     5161 cgggcggcat gccagacggc atcctcctcg acgaccaggg tgctcatgag ctcccccggc
     5221 atcccgtgcc ggccttcgcg ctcgagattc accttccgga accagcgtcg aggcgtgacc
     5281 gtgatgctcg cgtagcgcac ccattcgagg aagaattcct tcccctcacc atggccgcgc
     5341 ccgccgccaa cgccgatctc ctcggtcgcc aggtcgagca gaccggagat atctcgcgcg
     5401 ccgagctttt catggaacgt ccgcacaaca tcgatatcgt gattctcgct catgatcgct
     5461 tcccgttttc ctgttgcgac gccggatgct cggtcggatc gcacacgtgg cgaaggtcgt
     5521 ggacagcgtt gtctccctcg gtataatcct cccaaagatc agccccgaca accgaacgta
     5581 ctccggaaag gacgcctcgt gaagatcacg gacctcaagt gcgccgtcat cggcgacaac
     5641 ccgatcgttc gcatcgtgac cgacgaaggg atcagcggct acggcgaggt cgaatcctac
     5701 aagccctacc tcgcaccgat cgtcctcatg ctgaagccgg cgctgatcgg cgaggatccc
     5761 acgaacgtcg agcgggtgat gcggaagatc cgcacgcggg gtggcttcaa gccgtggggc
     5821 gcggccgtca gcgccgtcga gatggcgctc tgggacgtgg cgggaaaggc cgcgggtctc
     5881 ccggtctaca agctgctcgg cggcaaggtc cgggacaagg tgcgtgccta caacggtgcg
     5941 atgcgaacgc cgctcgcggg ctacacgccg gaggattacg ccgcgagcac ggcggcgatg
     6001 aaggctcgac ccgagaactt caggctgatc aagcaggcga tcgcctttca cacgccgatg
     6061 tatgccgaga tcccggattt cttctatggc gagccacagc agcgggcatt ccacagccac
     6121 atcaatcgcg gcctgatgac ggagaagggg ctcaagcacg tcatcgcctg cgtcgaggcg
     6181 atgaaggagg tcctcggcga cgaggtcggg ctcgcgctcg actgcggtcc cggctggatg
     6241 ctgccggacg cgatccgctt cgcgaaggcg gtggagcacc tcaacatcac ctggctcgag
     6301 gatcttctca ccggcgacta cgtccactgg gtgaatgccg acgtctatcg cgaattgacg
     6361 acgagcacgt cgacgccgat ccacaccggc gagcagatct acctgcgtca caacttcaag
     6421 cagctgatcg agacccacgc ggtgaacgtg atcggcccgg atccgggcga tgtcgggggt
     6481 atcgccgaac tgaaatggat cgcggagtac gctgatctcc acggcatcct gatggctccc
     6541 cacggtaccg gcaacggcct gttgggactg gcggcgctgg tccaggtctg cgcgaccctg
     6601 cccgacaact acgtcgcctt cgagacaccg accgggaaac cggaatggtg gttcgacatc
     6661 gtcgacggtc ttcctgatcc gctgatcgtg gatggaacga tcgaggtctg ggatcgtccc
     6721 ggcatgggta tcgagctgaa cgtcgagaag gcggccaagt acctctccgc cggtgatgcc
     6781 gacttcttcg cctgaaccgg gctcccattc ccgtccccac ggcgcttgtc gccgtttcgg
     6841 atcggctgcg gctagacatg acatccaaca ctgtcgcgta aaggttcgtc atttcgagcc
     6901 tgtcgagaaa tccccgtgcg aagcacatcc ggcccgttcg acaggctcag ggtaaactcc
     6961 gccgggcccg aacacggcca ttccatgcaa cacgcgctgc ggaaacggat cggttgcgcc
     7021 gcattgaccc gggacgagaa cgaaccgcgc accgggagcg tcgaccgcga cccgttctcc
     7081 gctccgcccg gttcatgcga cggcgttgga gatgaacgcc cgctccacaa acccggtggt
     7141 tccaccacgt cccgttcgcg cggcccgttt gtctgcccc
//
LOCUS       DASTMD010000092         3541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2776234, whole genome shotgun sequence.
ACCESSION   DASTMD010000092 DASTMD010000000
VERSION     DASTMD010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2776234"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFQ54_01435"
     CDS             complement(<1..264)
                     /locus_tag="VFQ54_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163672.1"
                     /translation="MSSPTNKLSRRAFTGAAAGAAALAAAPLLRAGAQGASPSPVAQG
                     ASPSPVASAPLAVGTPEDQLEIFSWWTSGGEAAGLQKLFDSFTV"
     gene            complement(731..2308)
                     /locus_tag="VFQ54_01440"
     CDS             complement(731..2308)
                     /locus_tag="VFQ54_01440"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEU0163673.1"
                     /translation="MTRESTTTLVGQSMMFRFEGPVFTAGAQEAFREIRPGGVIFFGD
                     NITSRTQIHALTAELRDHAAKLGMPPLFIAADQEGGIVSRFSPDMTTVPGAMALSATG
                     ELADIRTSARITGEQLAAVGINTNFAPVVDVNNNPVNPVIRTRSFGDTVEQVIAGSLA
                     AIDGLNDAGIIGTVKHFPGHGDTSVDSHLGLPVVNHSRERLDAVELAPFRAAIAAGVP
                     GVMTTHILFPELDQDPATLSRAILTGVLRNDLGFDGVIFTDSLSMAAIEARYGHGDAA
                     IRCKLAGVDVLEANESIAHQRSRFHALIDAARDGTVPLAIFERTVERLVALRSRFAIG
                     RDPGPLGGADPAWDTTAREIAARAIVALPEGGFLPIRPDANLGVIDFQRLRATEAEDP
                     FNRAGVFRTAAMETFTNARVVTLGNTPRQEEIDEAISLASDVETLVVMTRDAALNPNQ
                     VEIGTQAISRAGEETRIIHVALRGPYDRGIFGDVDATILTFGDPAVTLRVLPGALGGA
                     TSITARLPIRLGGATDR"
     gene            complement(2622..3257)
                     /locus_tag="VFQ54_01445"
     CDS             complement(2622..3257)
                     /locus_tag="VFQ54_01445"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0163674.1"
                     /translation="MAEAAAAEGSAGGSRKGAAKRGPYAKSAGRRREIIDEVLSVYNQ
                     RGIDGTSLRAIARAIGVTHPVLVHHFGTREELFLEVLREYDERHLALLRESGVLREFI
                     DSAADYSISEPGLMALLNSMVALALETDNEHSRARFSARYAELRELLVRLLQDGQRAG
                     TVRSDIPLEDTASLLIAAADGLTTQWLLDGAADFKRGLQLLTRLLEPPVTA"
BASE COUNT          647 a         1074 c         1193 g          627 t
ORIGIN      
        1 taccgtgaag gaatcgaaca gcttctggag gccggcggcc tcaccaccgg atgtccacca
       61 ggagaagatc tcgagctgat cctccggtgt gccgaccgca agtggcgcac tcgcgacagg
      121 agagggactg gcgccctgcg caacgggaga gggactcgcg ccctgggcgc cggcgcgcag
      181 caacggcgcg gcggcgaggg cggccgcgcc ggcggcggct ccggtgaagg cgcgacggga
      241 aagtttgttc gtgggggaag acatgtccag gaaacctccg tgaaccggga ccggcaacgc
      301 tgccgggatt ccgggcgaaa ctatcgaatg cgtgcgcatc aatacgaatc ggactcgacg
      361 acgtggtcga gtgacgggag tgagtgggct ggagattccg aacgaagcgc gcagttcgtc
      421 accggggaaa tgcaccaacg cctggaggac cctcctttcg gaaattgagg taccaaacat
      481 accggtgaag caacggcgtg cgtgcgatct ctgcggaatc tgggcgaagt atggggacgt
      541 cggggcaatc ttttcaatgt cgttggcaga attacaatat gttgtcaacg atttccgaac
      601 gtgagcctcg gccgtggagc tactccaaca ggtcacctgg cgcagtttcg tgtctcctgg
      661 cgcccgggag cggacgcttc gctggtcagg cgagtcgctc gccgatcgtg acccaaggaa
      721 aacgaatgat ctaccggtcg gtcgcgccgc caagtcgaat cgggagcctt gccgtgatcg
      781 atgtcgcccc gccgagggcg ccaggcagaa cgcggagcgt caccgcggga tcgccgaagg
      841 tcaggatcgt cgcatcgacg tcgccgaaga tgccgcgatc gtaggggccg cgcagcgcga
      901 cgtgaatgat tcgtgtttct tcgcccgcac gggagatcgc ctgggtgccg atctccacct
      961 ggttgggatt gagcgcggcg tcgcgggtca tcacgacgag cgtctccacg tcgctcgcga
     1021 gcgagatcgc ctcgtcgatc tcctcctggc ggggggtgtt gccgagcgtg acgaccctgg
     1081 cgttcgtgaa ggtctccatc gccgcagtcc ggaagacgcc cgcgcggttg aaggggtctt
     1141 cggcttccgt cgcgcggaga cgctggaagt cgatcacgcc cagattcgca tccgggcgga
     1201 tcgggaggaa gccaccttcc ggcagggcga cgatcgcgcg cgccgcgatc tcgcgtgccg
     1261 tggtgtccca cgcgggatcg gcgccaccga gtggtcccgg atcgcggccg atcgcgaatc
     1321 gggagcggag cgcaacaagc cgctcgactg ttcgttcgaa aatcgcgagc ggaaccgtgc
     1381 cgtcacgcgc ggcgtcgatc agggcgtgaa accggctgcg ctgatgggcg atcgactcgt
     1441 tcgcctcaag gacatcgacg cctgccagct tgcagcggat cgcggcgtcg ccgtggccgt
     1501 agcgagcctc gatcgcagcc atcgacagcg aatccgtgaa gatcacgccg tcgaagccga
     1561 ggtcgttccg gagaaccccg gtcaggatcg cccgggaaag cgttgccggg tcctgatcga
     1621 gctccggaaa gaggatgtgg gtcgtcatca caccggggac tcccgccgcg atcgccgccc
     1681 ggaatggcgc gagttcgacc gcatcgagac gttcccgcga gtgattgacg acgggcagcc
     1741 cgaggtggga atcgacgctc gtatcgccgt gaccgggaaa gtgcttcacc gtcccgatga
     1801 tcccggcgtc gttcaggccg tcgattgcag ccagcgaacc cgcgatcacc tgctcgacgg
     1861 tatcgccgaa cgaccgtgtg cgaatgacgg gattcacagg gttgttgttg acgtcgacaa
     1921 ccggcgcgaa gttggtgttg atgccgacgg cggcgagttg ctcgccagtg atcctcgcgc
     1981 tggttcgaat gtccgcgagt tccccggttg ccgagagcgc catcgccccg ggcacggtcg
     2041 tcatgtccgg cgagaagcgg ctgacgatgc cgccttcctg atcggcggcg atgaagagtg
     2101 gcggcatccc gagctttgcc gcatggtcgc ggagttcggc ggtgagtgcg tggatctggg
     2161 ttcggctcgt gatgttgtcc ccgaagaaga tgacgccgcc cgggcgaatt tcgcggaacg
     2221 cctcctgcgc cccggcggtg aagaccggtc cctcgaagcg gaacatcatg ctctgaccga
     2281 cgagcgtggt agtggattcc ctggtcatga tcgtccttca ggcgtaacgc gaatctgctg
     2341 ccccagtatc cgggcatcgt gctgacgacg cccgcggccc gatcggcgtt ggagtccggc
     2401 ggagtttttg ctctgagcct gtcgaatggg ccggatgtgc ttcgaactgg tccccagaag
     2461 ttggactgag aaagtcagat caattctggg agcagtatca tgggtgacgg aagatccttg
     2521 tccgaggcgg agcgacgggc agccgtcgcc ttgttcgcgg cgagctcggg acgatgctac
     2581 tgcaaaaata taggtgtgga tagtttggga ttggcgaact tctaggcggt gacgggaggt
     2641 tcaaggaggc gtgtgagcag ctgcagcccc ctcttgaagt ctgcagcccc gtccagcaac
     2701 cattgggtcg tcagcccgtc ggcggcggcg atgagcagag aggcggtgtc ctcaagcggg
     2761 atgtcggagc gcacggttcc ggccctctgg ccgtcttgca acaggcgcac gagaagttct
     2821 cgcaactcgg cgtaccgcgc agagaaacgt gctcgagagt gctcattgtc ggtctcgagc
     2881 gctagcgcca ccatgctgtt aagcaaagcc atgagcccgg gctccgagat gctgtagtcg
     2941 gcagcgctgt cgatgaactc gcggagcaca ccgctttcac gcaaaagtgc caagtggcgc
     3001 tcgtcgtact cgcgcagtac ctccagaaag agttcctccc gcgttccgaa atggtgaacg
     3061 agcaccgggt gggtcacgcc gatcgcccgt gcgatagccc tcaacgacgt tccgtcgata
     3121 ccgcgctggt tgtacaccga gagcacctca tcgatgatct cccgccgtcg gccggcactc
     3181 ttggcatagg ggccgcgctt ggcagcaccc ttacgagatc cacccgcgga gccttccgcc
     3241 gccgccgcct cagccatcgg ccgcctccca ccatgttcca gattgcaacg atcgaaatat
     3301 ccacacggat attttgtgat attgtaatgc ggcaaggccg cgtccgacgg cgatccacaa
     3361 gggccgacat tcgagtgtcg gtcatgattc aacggagaac aacgtgactg gcattgtgca
     3421 atccccaacc gctgagttcg cccgcttcga gcggagcacg tccgaccccg actggtggcg
     3481 tcaagcggtc gtctaccagg tgtatccgcg gtcgttcgca gacgccaacg gcgatggcgt
     3541 c
//
LOCUS       DASTMD010000093         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_8508, whole genome shotgun sequence.
ACCESSION   DASTMD010000093 DASTMD010000000
VERSION     DASTMD010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8508"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..621)
                     /locus_tag="VFQ54_01450"
     CDS             complement(349..621)
                     /locus_tag="VFQ54_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate o-methyltransferase 1"
                     /protein_id="HEU0163675.1"
                     /translation="MVDDRSLTEAEATRIANLAVDEIGGPRSVYRNPRMAFSQQARRE
                     VEIEGWKVDIRYGEISTPAIATVGGWVFEIHDEEIELLMRPPKPRR"
     gene            824..1174
                     /locus_tag="VFQ54_01455"
     CDS             824..1174
                     /locus_tag="VFQ54_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163676.1"
                     /translation="MVTLARFRPDIDEFPSGVTVDCRWRHEYPEPGSHHAATTRSVAG
                     GKLTPGAGGNHAVGKDLSQVTGERPGSYLSIGSVCVEHRIDQTRHQPAPDKVALVADI
                     VTRVDRVLMTLDSP"
     gene            1233..2438
                     /locus_tag="VFQ54_01460"
     CDS             1233..2438
                     /locus_tag="VFQ54_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0163677.1"
                     /translation="MVTTPNTLKSEIDEILPGVIADRRHLHEHPELGFQEHETAAFVA
                     QRLQTLGVEDIRTGINKTGVTGLIHGTKPGAGKVVLLRADMDALPITEENEVEYVSQN
                     PGVMHACGHDAHTSILLGVARILMEKRSLFSGTVKVLFQPSEELFPGGAVGMIAEGVL
                     EDPHVDAVFGLHMAQESPVGTVLISAGPIMAAPDSFKIDIQGKGGHAARPQAAIDPVV
                     IGSEIVVAIQTLVSRETDPMDNAVVSTTYFNSGDAFNVIPDIASLGGTVRTFKPETRD
                     YMEKRLPELVEGVGAALGAKVEATFTRGYPATVNDPEQAERVRQAAIKVVGEEKVLPK
                     KPAMGGEDFSYFLEQRPGAYFFVGTKNEEKGLIWGHHHPRFDIDEESLGVGIETTVET
                     VFSYLNDEA"
     gene            2442..>2758
                     /locus_tag="VFQ54_01465"
     CDS             2442..>2758
                     /locus_tag="VFQ54_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163678.1"
                     /translation="MVTTTAIKSEIDEILPGVIADRRHFHENPELAFQEFETAKIVAS
                     RLESLGVEDIRTGINITGVTGLIRGTGTGPNADRVILLRAYMDALPILEENEVDYVSQ
                     NPGV"
BASE COUNT          507 a          972 c          813 g          466 t
ORIGIN      
        1 cgccgcacgg atgacggcgg cgaacatggt gtcccgccac agccgagagc ttcaccacgc
       61 gccgccttac gcgtcacgat cactgtcgga gatatggggg gtatgggcac cgtccacgga
      121 cgtggcaccg ggcgaccacc agggtcgccc ctacggagtt accgaccatc gtaggggcga
      181 ccccggtggt cgcccactgt tgggcatccc cgtctctggc gagcacccaa cgtcgacagg
      241 accgtcctcc cgagcttgtc gagggatcgc atgggtgcca catgttcccg gcacgacgct
      301 gtatcgtcat tctgagccaa cggcgaagga tccccgcaca cttcaggatc acctccgcgg
      361 tttcggggga cgcatcagga gttcgatctc ctcgtcgtga atctcgaaga cccacccgcc
      421 aaccgtcgcg atcgccggcg tcgaaatctc gccgtagcga atgtcgacct tccagccctc
      481 gatctcgact tcgcggcgcg cctgctgact gaatgccatt cggggattgc ggtagacgct
      541 ccgcggtccc ccgatctcat cgaccgccag gttcgcgatc cgcgtcgcct ccgcttccgt
      601 caagctccgg tcatcaacca tggttccctc attcgtcctg gattgccaaa caacgccccg
      661 gcatcgctgt gcaacccgac cagttcacga aatcgcctgc atctggtacc ttgctccgaa
      721 atcgtcgacc gtcgagtctg cgcgacatcc gcttcccgcc ttttgagcga atcgcacagc
      781 gaccgtcgac ccacccgccg tatcccagga gtactcgccg cgaatggtaa cgctggcacg
      841 ctttcgacca gatattgacg agttcccctc tggcgtcacc gtcgactgcc gctggcgtca
      901 cgaatatccc gaacccggct cccaccacgc cgcgacgact cgctccgtcg ccggcggcaa
      961 gctcacgccc ggtgccggcg ggaatcacgc cgtcggcaag gacctctcgc aggtcaccgg
     1021 ggaacgccct ggatcatact tgtccatcgg ttccgtctgc gtcgagcacc gcatcgacca
     1081 gacccgacat caaccagcgc ccgacaaggt tgcgctcgtc gccgatatcg tcacgagggt
     1141 cgatcgcgtc ctgatgacgc tcgactcgcc gtgacgtcga tcgtttcgcg tagcagttct
     1201 tttcatctcg atgtaccccg gaaggaatac ccttggtcac cacgccgaac acgttgaagt
     1261 ctgaaatcga tgagatcctg ccaggggtca ttgccgatcg ccgccacctc cacgagcacc
     1321 cggaactcgg cttccaggag catgagaccg ccgccttcgt cgcccagcgc ctgcagacgc
     1381 tcggggtcga ggacatccgg accgggatca acaagaccgg cgtcaccggg ctgatccacg
     1441 gcacgaagcc gggcgccggc aaggtcgtcc tcctgcgcgc cgacatggac gcgttgccga
     1501 tcaccgaaga gaacgaggtc gagtatgtct cccagaaccc gggcgtgatg cacgcctgcg
     1561 gccacgatgc ccacacctcg atcctgctcg gcgtcgcccg catcctgatg gagaagcgca
     1621 gcctcttctc cggcaccgtc aaggtgctct tccagccgtc cgaggaactc ttccccggcg
     1681 gcgccgtcgg catgatcgcc gagggcgtcc tcgaggatcc gcatgtcgat gccgtcttcg
     1741 ggctccacat ggcgcaggaa tcgccggtcg gcacggtcct catctcggca ggaccgatca
     1801 tggcggcgcc ggacagcttc aagatcgaca tccagggcaa gggcggtcat gccgcccgtc
     1861 cgcaggcggc gatcgacccg gtcgtgatcg gttccgagat cgtcgtcgcg atccagacgc
     1921 tcgtctcccg cgagaccgat ccgatggaca acgccgtcgt cagcacgacc tacttcaact
     1981 ccggcgacgc cttcaacgtg atcccggata tcgcatcgct cggcggcacc gtccgcacct
     2041 tcaagccgga aacgcgcgac tacatggaga agcggctccc cgagcttgtc gagggtgtcg
     2101 gcgcggcgct cggcgccaag gtcgaggcca ccttcacccg cggctatccg gcgaccgtca
     2161 acgatccgga gcaggcggaa cgcgtccgcc aggccgcgat caaggtcgtc ggcgaggaga
     2221 aggtcctccc gaagaagccg gcgatgggcg gcgaggactt ctcctacttc ctcgagcagc
     2281 gcccgggcgc ctacttcttc gtcggcacca agaacgagga gaagggcttg atctggggtc
     2341 atcaccaccc gcgcttcgat atcgacgagg agtcgctcgg agtcgggatc gagaccacgg
     2401 tcgagaccgt cttcagctac ctgaacgacg aggcctgacc gatggtcacg accaccgcaa
     2461 tcaagtcgga gatcgacgag atcctgcccg gagtgatcgc cgatcgccgt cacttccatg
     2521 agaatccgga actcgccttc caggaattcg agaccgcgaa gatcgttgcc tctcgtctcg
     2581 aatcgctcgg tgtcgaggac atccggaccg ggatcaacat caccggcgtc accgggctga
     2641 tccggggaac ggggacaggc ccgaacgccg accgcgtgat cctgctccgc gcctacatgg
     2701 acgcgctgcc gatcctcgaa gagaacgagg tcgactacgt cagccagaac ccgggtgt
//
LOCUS       DASTMD010000094         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2954970, whole genome shotgun sequence.
ACCESSION   DASTMD010000094 DASTMD010000000
VERSION     DASTMD010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2954970"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1163
                     /locus_tag="VFQ54_01470"
     CDS             228..1163
                     /locus_tag="VFQ54_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163679.1"
                     /translation="MTPYVPNWGKTPAAGPGLTTPLSDQSSPEATGGTETTPSDNQKS
                     PSGTDGDLSSASPDSSVAPIEPGGRLYVDDSQDVLGSTVDPSDASTSTSGLTSDQLQW
                     PKGETGTVVGWRDGVVFYDRQDDQGQISYFIADVDGSGNLSGEQNLGSDPGKAWLPTG
                     APAYRYQDGIVVQTAGGWVVATSHGARFTKSINPGETLTTVRVDGVVSPSGFIAFQTS
                     GGIEIDYLDSGESTVVDVGNVVDFDLSGDGSQLVISTGSDIEIWSVDGGQHVSRYANS
                     QGISVGSVLWYGDTIYFVDTQTNAVMTIGLPTGGQ"
     gene            1327..1965
                     /locus_tag="VFQ54_01475"
     CDS             1327..1965
                     /locus_tag="VFQ54_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEU0163680.1"
                     /translation="MRPDELSHLPGETGESGDVHADEEAVRFGRAIIIASARPILDQP
                     VAAIVCPANRRGVMGVGLSGLVRTTGGIEIEREAMALAPLTIGTAVATTSGNLAARGV
                     RVILHAVVSDALGSATRADIVRRATVSTLQAVDRQRLKSVAFPPLGSGLASIGLTSMM
                     VLAYMIDEIVAHLRRFTSRLERIVLVCRDQREVREIGAALREVRQDWDGLRA"
     gene            2023..>2639
                     /locus_tag="VFQ54_01480"
     CDS             2023..>2639
                     /locus_tag="VFQ54_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163681.1"
                     /translation="MTDRDQNEFRYQPPGDGASMARMGDSPAPVPGVVDAAPAIRQDA
                     VSIRAWRYQPDAWRWVKRFNHRYANSVLRLRSAKVMPASIRARFSQMGLPPEIIEETL
                     ASIRKLDQWSDAWTETAQLFMGEYRRQISAKNDDQAAKSQWLAAMSYHAAQILTVPGD
                     ERTAVTCRAAAASLFRHAQPWIYPKIRRLEIPWRTGVLPAYMQVP"
BASE COUNT          467 a          839 c          865 g          468 t
ORIGIN      
        1 catcgttttt gcaggcttat ctggccggac gtacagggca aagtgccgag gaagtgatgc
       61 cacttcttga agagctgccg gtaggatgga gtctcgatag ggaaacaacc gacatcacag
      121 acgaggtcaa cacaatccag cctactcccg cacccccttc gccaacggcg acaacggtcc
      181 ctcggccgac aacagcccct cggccgacgc ggacaccgta cccaacggtg actccctatg
      241 ttcccaactg ggggaaaact cccgctgccg gtccgggcct gacaactccg ctttcggatc
      301 agtcgagtcc ggaagcgacc ggtggcacgg agacgacgcc gagcgacaac cagaaatccc
      361 caagcgggac cgacggcgat ctctcctcgg cctccccgga cagcagcgtg gcgccgatcg
      421 agccgggcgg tcgcctctat gtcgatgaca gccaggatgt cctcggctcc acggtcgatc
      481 cgtccgatgc ctccacctcg acgtcggggc tcaccagcga tcagctccag tggccgaagg
      541 gcgagaccgg gacggtcgtc ggctggcgcg atggggtcgt cttctacgat cgccaggacg
      601 accaggggca gatcagctac ttcatcgccg atgtcgacgg gagcgggaat ctctccggcg
      661 agcagaatct cgggagtgat cctggcaagg cgtggctacc gaccggtgcc cctgcctatc
      721 gctatcagga tggcatcgtg gtgcagaccg cgggcggctg ggtcgtggcg acgtcgcacg
      781 gcgcacgctt cacgaagagc atcaatccgg gggaaacgtt gacgaccgtg cgcgtcgatg
      841 gcgtggtcag tccctccggc ttcatcgcct tccagacgag cggcggcatc gagatcgatt
      901 atctcgacag cggcgagtcg acggtggtgg atgtggggaa cgtggtggac ttcgatctct
      961 ccggcgacgg cagtcagctg gtgatcagta ccggcagtga tatcgagatc tggtcggtcg
     1021 acggtggaca gcatgtgagt cgatacgcga acagccaggg tatctccgtc gggagcgtgc
     1081 tctggtatgg ggacacgatc tacttcgtcg acacccagac gaatgccgtg atgacgatcg
     1141 gccttccgac cggcgggcag tagcggcgtc gattgtcgag gagggagtcc acccgggtgg
     1201 actccctcca tttttcggag aagtacggtt cggatgtttc agatccgtgt cgcaaaactg
     1261 atagactcca gagttgttct tggtgaacgc gcttcgcgag gaaattgctc tggaggtttg
     1321 ggcgcgatgc ggccggacga actatcccat cttcccggtg agaccgggga atccggtgac
     1381 gtccacgcgg atgaggaagc cgtacgtttt gggcgggcga tcattatcgc ctcggcccga
     1441 ccgattctcg atcaacccgt cgccgccatc gtctgcccgg caaaccggcg gggcgtgatg
     1501 ggcgtggggc tctccggtct ggttcgcacc accggcggaa tcgagatcga acgcgaggcg
     1561 atggcgttgg cgccgctgac gatcggcacc gcggtcgcga ccacgtccgg caatctcgcg
     1621 gcccgcggtg tccgcgtcat ccttcacgcg gtggtctcgg atgcgctcgg ctccgcgacg
     1681 cgtgcggaca tcgtgcgccg cgccacggtc tcgaccctgc aggcggtcga tcggcaacgc
     1741 ctgaaatcgg tggcgtttcc gccgctcggt tccggtctcg cctcgatcgg gctcaccagc
     1801 atgatggtgc tcgcctacat gatcgatgag atcgtcgctc acctgcggcg gtttacctcg
     1861 cggctggagc ggatcgttct ggtctgccgc gatcagcgcg aagtccggga gatcggcgcc
     1921 gccttgcgag aggtacggca ggactgggac ggacttcgcg cctaggtcaa tggacgcttc
     1981 ggttcgggat agggacgtca tcatgacaag aatcgatgca ctatgactga tcgcgaccag
     2041 aacgagttcc gataccagcc gcccggcgac ggcgcaagca tggcgcggat gggcgactcg
     2101 cccgcaccgg tacccggcgt cgtcgatgcc gcccccgcga tccgccagga cgcggtctcg
     2161 atccgcgcat ggcgctacca gccggatgcc tggcgttggg tcaagcgctt caatcatcgc
     2221 tatgcgaatt cggtgctgcg cctgcgatcg gcaaaggtga tgcccgcgtc gatccgtgcg
     2281 cgcttctcgc agatgggact tcccccggag atcatcgagg agacgctcgc ctcgatccgg
     2341 aagctcgacc aatggtcgga cgcctggacg gagaccgcgc agctcttcat gggtgagtac
     2401 cggcggcaga tttcggcgaa gaacgacgat caggccgcca agtcacaatg gctcgccgcg
     2461 atgagctatc acgccgccca gatcctgacc gtccccggcg acgagcgaac ggcggtcacc
     2521 tgccgcgccg ccgcggcgtc gctctttcgc catgcccagc cctggatcta tcccaagatt
     2581 cgccgcctcg agatcccgtg gcggaccggg gtgcttcctg cgtacatgca ggtgcccga
//
LOCUS       DASTMD010000095         5150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1079510, whole genome shotgun sequence.
ACCESSION   DASTMD010000095 DASTMD010000000
VERSION     DASTMD010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079510"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="VFQ54_01485"
     CDS             complement(<1..1235)
                     /locus_tag="VFQ54_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEU0163682.1"
                     /translation="MSLDVQSYMSAVPEMDRFLTVDELHADLDRIVATYPEIARLERV
                     GSSTLGEPIRMLSIGSGSKNIFLFGCPHPNEPIGAMMLHHLSEKLCTDATLREAYDYT
                     WNLIPTIDPDGTRLNEGWFAGPFTPTHYARNFYRPAGPQQVEWTFPNSYKLNYFDASL
                     RETQTLMRVIDQLKPEFMFSLHNAGFGGVYYYLTREAPDLYPVFAELPTWEELPLRLG
                     EPEMPYVTEYAPAIFHLITAENTYDYLEANGADMTNSHHGGSSAQYAEPYGTLGLVTE
                     MAYFDDPRVNDLTVTETKRRDAILAGLDLSDEGTTVVSGHFDAVKGELRTKSTFEVAV
                     GWFAESAKKSKGAQRHWAETDPETDRPATVAELFSSRQEVQFYRLLTLGMLVRMLEGE
                     VAVGNGTPIIRKHLKEARST"
     gene            complement(1407..3017)
                     /locus_tag="VFQ54_01490"
     CDS             complement(1407..3017)
                     /locus_tag="VFQ54_01490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0163683.1"
                     /translation="MTSRSSRLSRQLQTSESPDGKSAFSSPAMNAALFCVLLGAIDLT
                     VIAAVLPGMIADLRVNTSDIDRYIWIVSGYLIAYIVAIPLIGRVSDFAGRRFAFLLAI
                     AVFLVGSIVCALANSLDSLIVGRCIQGFGGGGLLPVAIALTGDTLPRRRRLAGIALVS
                     AVDTLGWVIGPVYGALIDSFAPGATEHWRWIFWINIPLLLVAAALVARAFPAPEPGES
                     LRALRRLDLPGAVFLTGLLVAVNLALSTGGELGSQTGSGLRALGGTTNPLARYVWLFV
                     VLAVVCVLLLAVTQRRSASPILPVTLLRNRTFLFAGISNFVLGVVLMVGMVDVPVVVT
                     LLHTSGSSSSSNVSAAILASYTICIMATSLASARLIRRFGLMIVMLGGLVCAGGGLAL
                     LYPLLEGDHVWRMIPGLVVAGCGLGTLIAPLSSAALDEANEEERGAAASTSLVFRLLG
                     MTIGISALSSIAVKRLQVLTDRLPPVSQQPNESTAAFLLRQQQFLQDRVIPLAVQVIQ
                     ETFLIAAALTLLALIPVIRIARTNRSKP"
     gene            complement(2974..3714)
                     /locus_tag="VFQ54_01495"
     CDS             complement(2974..3714)
                     /locus_tag="VFQ54_01495"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEU0163684.1"
                     /translation="MTSRSIKSASILAVLSLFFMSWGTGGNVLAASDQTPPAADVLAS
                     ASKSLASTKTAHFVLKIDGDTYLDAAKSLKLISAEGDILRPDRVSVQFQVQVLGAGNV
                     SIKMITIGDQSWTTDLITGKWGAAPSEFGYNPAILFDAKSGLGPIMDKLTDAKVEGTE
                     KVDGNEAYKITGTSTAGEIKDVTSGTMTGTSFPVEIWIDTKTSKPLKITLAESSDSGK
                     EHPATWTMTLSGYDHDVSIESPVQATPD"
     gene            4019..4933
                     /locus_tag="VFQ54_01500"
     CDS             4019..4933
                     /locus_tag="VFQ54_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEU0163685.1"
                     /translation="MSLIQTDLDLVVSSVTESAGRPTNEDAILVATLPDAANPSESGY
                     VLAIADGMGGLERGEVASKLAIDLLADLFARERPSDIALALKQAYRRANDAIYKEIET
                     NPGEGEIGTTLVSAIVHGKYVTIANVGDSRAYLMRANQITQITQDHSVVAEQVSQGRI
                     SAQEARTSPQRNVLTHALGTAPQLDRRLPSIYELTLLPEDRLLLCSDGFYDVMQSEDY
                     LRVLGSAASSESARQLSALAQERGTTDNVSAIVVSVSPSLATVQRAQIGTEIASQAGS
                     RFGAIVFPAIVLLIVIVAIAVGAIVYLM"
     gene            4943..>5150
                     /locus_tag="VFQ54_01505"
     CDS             4943..>5150
                     /locus_tag="VFQ54_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163686.1"
                     /translation="MTDIAGSLTTPEGTAGTRGIIDAHTHLFPDEVIRDRARFLAADS
                     WFGETYGHPKAIAASVDLLIASMDD"
BASE COUNT          968 a         1505 c         1751 g          926 t
ORIGIN      
        1 gtggagcgtg cttccttgag gtgttttcga atgatcggcg tcccgttgcc gacggcgact
       61 tcgccctcga gcattcgaac gagcatgccg agggtcagga gccggtagaa ctgcacttcc
      121 tgcctgctgc tgaacagctc ggcgacggtc gcggggcgat ccgtttccgg gtcggtttcc
      181 gcccagtgtc gttgcgcgcc cttgctcttc tttgcggatt cggcgaacca gccgacggcg
      241 acttcgaacg tcgacttcgt gcggagctcg cccttgaccg cgtcgaagtg gccggagacg
      301 acagtcgtgc cttcgtccga gagatcgaga ccagcgagga tcgcgtcgcg acgcttcgtt
      361 tcggtgacgg tcaggtcgtt cacgcgggga tcgtcgaagt aggccatctc ggtgacgagt
      421 ccgagcgtgc cgtatggctc ggcatactgc gcgctcgacc cgccgtgatg ggagttggtc
      481 atgtcggcgc cgttggcctc gagatagtcg taggtgttct cggcggtgat caggtggaag
      541 atcgcagggg cgtactcggt gacgtacggc atctccggct caccgaggcg gagcgggagt
      601 tcctcccagg tgggaagctc cgcgaagacc ggatagaggt ccggcgcctc acgtgtcagg
      661 tagtagtaga cgccgccgaa tcccgcgttg tggagcgaga acatgaactc gggcttcagc
      721 tgatcgatga cgcgcatcag cgtctgcgtc tcgcgaagcg acgcgtcgaa gtagttcagc
      781 ttgtatgagt tcgggaatgt ccactcgacc tgctgggggc cggcgggtcg gtagaagttg
      841 cgggcgtagt gcgtcggcgt gaatggtccg gcgaaccacc cctcgttgag ccgcgttccg
      901 tcaggatcga tcgtcgggat caggttccac gtgtagtcat atgcctcgcg aagcgttgcg
      961 tccgtgcaga gcttttcgga aagatgatgg agcatcatcg cgccgatggg ctcgtttgga
     1021 tgaggacagc cgaagagaaa gatattcttc gagccgctgc cgatcgagag catccggatc
     1081 ggctcgccga gggttgacga cccaacgcgt tcgaggcggg cgatctccgg gtacgtcgcc
     1141 acgatccgat cgagatcggc gtgaagttcg tcgacggtca ggaagcgatc catctccggc
     1201 acggcggaca tataggactg gacatccagt gacatggtgg tctttcacga gggcgaacag
     1261 actgggccaa cggtggacgt ttttaccaaa cgcaccgcga cgatatctga gcccggaaag
     1321 actgtcaatt ggctcaatag ttgattcttg tgcgaaccgc acaaggatag ttgtgccgtc
     1381 atcctgcgct cacatggttt gcacaatcac ggcttggatc gattggtgcg cgcgatacgt
     1441 atgaccggaa tcagcgcgag aagggtcagc gccgccgcga tcaggaacgt ttcctggatg
     1501 acctggacgg cgagcgggat gacgcgatcc tggaggaact gctgctggcg gagcaggaag
     1561 gcggccgttg attcgttcgg ttgctgggag accgggggca ggcggtcggt caggacctgc
     1621 aaccgcttca cggcgatcga tgagagggcc gaaatcccga tcgtcattcc cagcaggcgg
     1681 aagacgagcg aggtcgatgc cgccgccccg cgctcctcct cgttggcttc gtcgagggcg
     1741 gccgagctca ggggggcgat cagcgtgccg agaccacagc cggcgacgac cagcccggga
     1801 atcatccgcc agacatggtc gccctccaga agcgggtaga ggagagcgag accgccgccg
     1861 gcgcaaacga gcccgcccag catcacgatc atgagtccga agcgccggat caagcgggcg
     1921 ctggcgagtg aggtcgccat gatgcagatc gtgtagctgg cgagaatcgc cgcgctcacg
     1981 ttgctggaac tggagcttcc cgacgtgtgg aggagcgtga ccacgaccgg gacatcgacc
     2041 atcccgacca tgaggacgac gcccagaacg aaattcgaga ttccggcgaa gaggaaggtg
     2101 cgattgcgga gcagcgtcac cggaagaatc ggcgaggcgg atcggcgctg ggtgacggcg
     2161 agcagcagga cgcacacgac ggccaggacg acaaagagcc agacgtatcg ggcgagcggg
     2221 ttcgtcgtgc cgccgagtgc ccgcaggccg gatcccgtct ggcttccgag ttcacccccg
     2281 gtcgagagcg cgagattgac ggcgacgagc agtccggtca ggaagacggc gcccggaagg
     2341 tcgagacggc gcagcgcgcg gagggactcg ccgggttccg gcgcggggaa cgcccgcgcg
     2401 acgagcgccg ccgcgacgag gaggagcggg atattgatcc agaagatcca ccgccagtgc
     2461 tcggtggcgc ccggtgcgaa cgaatcgatc aatgccccgt agacgggccc gatgacccag
     2521 cccaacgtgt cgaccgcact gacgagggcg atgccagcga ggcgacgacg cctcggcagg
     2581 gtatcgccgg tcagggcgat cgccaccggc agtagcccgc cgccgccgaa tccctggatg
     2641 cagcggccga cgatcagcga atcgagtgag ttcgcgagag cgcagacgat cgagccgacg
     2701 agaaagacgg cgatcgcgag caggaaggcg aaacgccgcc ccgcgaagtc cgagacgcga
     2761 ccgatgaggg ggatcgcgac gatgtaggcg atgaggtagc cgctgacgat ccagatgtac
     2821 cggtcgatgt cggatgtatt gacccggaga tcggcaatca tccccggcag cacggcggcg
     2881 atcaccgtga gatcgatggc gccgaggaga acgcagaaga gcgccgcgtt cattgccggg
     2941 gaggaaaacg ccgacttccc gtctggcgat tcgctagtct ggagttgcct ggacaggcga
     3001 ctcgatcgag acgtcatgat cgtatccgga aagcgtcatc gtccacgttg ccggatgctc
     3061 cttgccgcta tctgacgact ccgcgagggt gatcttcaac ggcttcgagg tcttggtgtc
     3121 gatccagatc tcgacaggga acgaggtccc ggtcatggtt cccgaggtga cgtccttgat
     3181 ttcgccggcc gtcgaggttc cggtgatctt gtatgcctca tttccgtcga ctttttccgt
     3241 gccctcgacc ttcgcgtccg tcagcttgtc catgatcgga cccagtccgc tcttcgcgtc
     3301 gaagagaatg gccgggttgt atccgaattc tgacggcgcc gcgccccact ttccggtgat
     3361 gagatcggtc gtccaggact ggtcaccgat cgtgatcatc ttgatcgaca cgtttccggc
     3421 gccgaggacc tgcacctgga actgcacgct gacgcggtcg gggcggagga tatcaccttc
     3481 agcgctgatc agcttgagcg acttggcggc gtcgaggtac gtgtccccgt cgatcttcag
     3541 gacgaaatga gcggtcttcg tgctggcgag gctcttgctc gcactcgcga ggacatcggc
     3601 ggcgggaggg gtctggtccg atgcggcgag gacgttgccg ccagtgcccc atgacatgaa
     3661 gaagagtgaa agaacggcga ggatggacgc tgacttgatg gatctggaag tcacggttgc
     3721 actcttgctc cggattggct ttcgaagcgc gtcggtgtgt tggcggccgc gacggaaccc
     3781 gtgtcgcttc gaagcaatct tatcagccgg agatggacgt gatttcaacg ttttgtcgcc
     3841 gttctgtgtc ggttcgatga acaggttgga gctttgcggg aacagcgtcg ataatgcggt
     3901 gacatccgga ttcagtctgg gatgccacca tggtcgtccg ctggtcatga atggcggcga
     3961 tcgatgagcc gaacgaaagc gcgtcgcaga cgcgctccag acataacgga gttccacctt
     4021 gagtctcatc cagaccgacc ttgatctggt tgtcagcagt gtcaccgaat cggcgggccg
     4081 cccgacgaac gaggacgcga tcctcgtggc gacgcttccg gacgcggcga acccgtccga
     4141 aagcggatat gtgctcgcga tcgccgatgg aatgggtggt ctcgagcgcg gggaggtcgc
     4201 cagcaagctt gcgatcgatc tgcttgccga tctcttcgcg cgcgagcgac cgtcggatat
     4261 cgcgctggca ctcaagcagg cgtatcgccg agcgaatgac gcgatctaca aggagatcga
     4321 gacgaacccc ggcgagggcg agattggcac gacgctcgtc tccgcgatcg tccatgggaa
     4381 gtacgtcacg attgccaacg tcggggacag ccgcgcttac ctgatgcgtg caaaccagat
     4441 cacgcagatc acgcaggatc attccgtcgt cgccgagcag gtctcgcagg gcaggatctc
     4501 cgcgcaggaa gcgcggacga gcccacagcg aaacgtgctg acccacgcgc tcggaaccgc
     4561 gccgcagctg gatcggcggc tcccgagcat ctatgagttg acgctgctgc ccgaggatcg
     4621 cctgctgctc tgcagcgacg gcttctatga cgtgatgcag tcagaggact atctccgggt
     4681 gctcggatcg gccgcgtcgt cggagtcggc gcggcagctc tcggcgctcg cccaggaacg
     4741 gggtacgacc gacaacgtct ccgcgatcgt cgtctcggta agcccgtcgc tggcgaccgt
     4801 gcaacgggcg cagatcggga ccgaaatcgc atcgcaggcg ggaagccgct tcggggcgat
     4861 cgtgttcccg gcgatcgtcc tcctcatcgt tatcgtggcg atcgctgtcg gcgccatcgt
     4921 ctacctgatg tagatgtaga tgatgaccga tattgccggg tcacttacca caccggaagg
     4981 aaccgcgggg acgcgaggca tcatcgacgc ccacacccac ctctttcccg acgaggtgat
     5041 ccgggatcgc gcgcggttcc tggctgccga ttcgtggttc ggcgagacgt acggtcatcc
     5101 gaaggcgatc gccgcctcgg tcgatctcct gatcgcgagc atggatgatg
//
LOCUS       DASTMD010000096         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2686839, whole genome shotgun sequence.
ACCESSION   DASTMD010000096 DASTMD010000000
VERSION     DASTMD010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2686839"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VFQ54_01510"
     CDS             <1..754
                     /locus_tag="VFQ54_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0163687.1"
                     /translation="PWTDIRVRQAFSHIVDRDAMKSQIWTSQANPAPSFLAPGFPASN
                     VEALKDIQAFDPDKAKQLLSDAGFPDGKGFKKLTITQRGGAATYEIATIQAYAAMVKQ
                     YLNIDTEIQEVDRDTFYANMKTIDFGFVSYGMDYFDASNMLGIWVTGGRHPWTNAEYD
                     KQVKAASVFGGAADERTKMFQAAEKILVTDVPAAFTFFITPNQLAKSYLTGPAFQPDH
                     NGIKAVHWPGYGTASTVPEELWLAADAPEGRS"
     gene            926..2131
                     /locus_tag="VFQ54_01515"
     CDS             926..2131
                     /locus_tag="VFQ54_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0163688.1"
                     /translation="MAATTTATDLVAAPELESPETLKSFYLLDPDITFLNHGSFGACP
                     RPVFEEYQAWQLELERQPVHFIGRRQEALLDAARARIGTYLNADPDSLSFVVNATSGL
                     NVIARSIELKPGDEVLTTNLEYGALDMTWDYLCKKSGATYVVQEIPVPFTTPQAVVDA
                     LWEGVNEHTRAIFLSHITSATATILPVAEICARARKEGILTIIDGAHAPGQIDLDMEA
                     LGCDIYSGNFHKWLQSPKGSAFLYVRPEHHDWIESLTISWGWVRGNSFVSRNQQQGTR
                     DVAAFLSVPAAIDFQEQHNWPAVRARAHQMLAMTRARMHEKLGTTPLYGGSADDDTTW
                     YNQLGLITLPKLEDRGAFQNRLLNHYGIEIPVSGHNDDWYFVRVSVQGYVTQKDLDTL
                     ESALIAELS"
     gene            2152..>2477
                     /locus_tag="VFQ54_01520"
     CDS             2152..>2477
                     /locus_tag="VFQ54_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0163689.1"
                     /translation="MTDARPSSPTIPLRVIGACPHDCPDTCAFVTEVRDGRAVKIEAR
                     KDHPVTQGWLCAKVSPYLERVYHEGRLTHPLRRVGPKGSGEWERISWDDALDEIASRW
                     KTIIAG"
BASE COUNT          494 a          856 c          723 g          404 t
ORIGIN      
        1 gccgtggacc gatatccgcg tccgccaggc attcagccat atcgtcgatc gcgacgcgat
       61 gaagtcccag atctggacca gccaggccaa tcctgcaccg agcttcctcg ctcctggttt
      121 cccggcatcc aatgtcgagg cactgaagga tatccaggcc ttcgaccccg acaaggcgaa
      181 gcagctcctt tccgacgccg gcttcccgga tggcaagggc ttcaagaagc tgacgatcac
      241 ccagcgcggc ggcgccgcga cctacgaaat cgcgacgatc caggcctacg cggcgatggt
      301 gaagcagtat ctcaacatcg acaccgagat ccaggaagtc gaccgcgaca ccttctacgc
      361 caacatgaag acgatcgact tcggcttcgt ctcctacggc atggactact tcgacgcatc
      421 gaacatgctc gggatctggg tgactggcgg gcgccatccg tggacgaacg ccgagtacga
      481 caagcaggtc aaggcggcct ccgtctttgg cggtgcggcg gacgagcgca cgaagatgtt
      541 ccaggcggcg gagaagatcc tcgtcacgga cgtcccggcc gcgttcacct tcttcatcac
      601 gcccaaccag ctggcgaagt cctacctgac gggaccggcc ttccagccgg atcacaacgg
      661 aatcaaggcc gtccactggc cgggctacgg gacggcgagc accgtgccag aggaactctg
      721 gctcgcggca gacgcgccgg aaggccgctc ctgatcgcgg aacggggagc gctgataccg
      781 gcgctcccac acttcacatc ctgaggcaat cgagagcatt gtgcggaacg agattcatcc
      841 gcacaatgct ctcactttgt gtgggtgctt tttggaaaga tcggcggcca cccggcaacc
      901 gatcatcgcg ttacaaggac gatgcatggc agcgaccacc acggcaaccg atctcgtcgc
      961 ggcaccggaa ctcgagagcc cggagaccct caaatccttc tacctcctcg atcccgatat
     1021 cacgttcctc aatcacggat cgttcggcgc ttgcccgcga ccggtcttcg aggagtacca
     1081 ggcgtggcaa ctcgaactgg agcggcagcc agtccacttc atcggtcgcc gtcaggaagc
     1141 actgctcgac gccgcccgcg cccgaatcgg aacctacctc aacgccgatc ccgacagtct
     1201 ttccttcgtg gtcaacgcga cgtccggtct caacgtcatc gcccgctcga tcgagctcaa
     1261 gcctggcgat gaggtcctga ccacgaatct cgaatacggc gccctcgaca tgacgtggga
     1321 ttacctctgc aagaagtccg gggcaaccta cgtcgtccag gagatccccg tccccttcac
     1381 gacgccgcag gcggtcgtgg atgccctctg ggagggcgtc aacgagcaca cgcgggcgat
     1441 cttcctcagc cacatcacct cggccacggc gacgatcctg ccggtcgccg agatctgcgc
     1501 gcgggcgcgc aaggaaggca tcctcacgat catcgacggg gcgcacgcgc ccggccagat
     1561 cgatctcgac atggaagccc tcggctgcga catatactcc ggcaacttcc acaagtggct
     1621 gcagtcccca aagggatcgg ccttcctcta cgtccgcccg gaacaccacg actggatcga
     1681 atcgctgacg atcagctggg gatgggttcg cggcaactcc ttcgtctcac gcaaccagca
     1741 gcaaggcacg cgcgacgtcg ccgccttcct ttcggtgccg gcggcgatcg acttccagga
     1801 gcagcacaac tggccggcgg tccgcgcccg cgcccaccag atgctcgcca tgacgcgagc
     1861 ccggatgcac gagaagctcg gcacgacgcc gctctatggc ggcagcgccg atgacgacac
     1921 gacgtggtac aaccagcttg gcctgatcac gctcccgaag ctggaggatc gcggcgcatt
     1981 ccagaaccgt ctcctgaacc actacgggat cgagatcccc gtcagcggcc acaacgacga
     2041 ctggtacttc gtccgcgtct cggttcaggg atacgtgacg cagaaggatc tcgacacgct
     2101 cgagtccgcg ctgatcgccg aactctcgta gcttctccga aggcatcgcc gatgaccgac
     2161 gctcgaccat cctccccgac cattccgctc cgcgtcattg gcgcctgccc gcacgattgc
     2221 cccgacacct gcgccttcgt cactgaggtg cgagacggac gcgccgtgaa gatcgaggct
     2281 cgcaaggacc atcccgtcac ccagggatgg ctctgcgcca aggtcagccc gtatctcgaa
     2341 cgcgtctacc atgaagggcg gctcacgcac ccgctccgtc gggttggacc gaaggggagc
     2401 ggcgagtggg agcgcatctc atgggacgat gcgctcgatg agatcgcctc caggtggaag
     2461 acgatcatcg ccgggca
//
LOCUS       DASTMD010000097         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_634355, whole genome shotgun sequence.
ACCESSION   DASTMD010000097 DASTMD010000000
VERSION     DASTMD010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634355"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFQ54_01525"
     CDS             complement(<1..341)
                     /locus_tag="VFQ54_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEU0163690.1"
                     /translation="MKTELLYRPSFAIARVELAQGESVRSEGGAMLSKSANVSIETSA
                     TGGLMKSLKRGILGGESFFMNTFRADGGTGEVIFAPSLPGDVVTVDIGTTPLLVNSGS
                     FLAAEMGVQVDT"
     gene            complement(535..1728)
                     /locus_tag="VFQ54_01530"
     CDS             complement(535..1728)
                     /locus_tag="VFQ54_01530"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU0163691.1"
                     /translation="MRQNTRKVSIRALVAAVIAAASLLMTMAMPFGTAAQSASSDWSA
                     PRTVYIPETGHTLDQLFLDMWRNAGGANAYGYPITNEITESNGHVVQYLQYARFEYWP
                     EGDADGNTAFVANLGDELRPMAVQRTVGTFSSQQSKTSSALDTARQIQAWFPVSASKF
                     NLDDGSVYYSETTKHAVQHGFLDYWQNVIGSSYLGEPLTEEYTLNGVTYQVFERGQLA
                     WTQEKDTWLVPVGQLLADKYKLNQAATDQGDAPTYSEDLFVPPPAPVASTPGGDCVDA
                     ELWVDVNLSSQSMDVYCGDQVILSTLVSTGRPGFETPTGEFFVNSKLPSQTMEGVIGG
                     EYYNVPDVPDVMYFTDEGHALHGAYWHNNFGNVMSHGCVNLPMDVADWLYSIAPVGMR
                     VEIHY"
BASE COUNT          417 a          673 c          719 g          421 t
ORIGIN      
        1 gtatcgacct gtacccccat ctcggcggca aggaaggacc cggaattgac gaggagcggc
       61 gtcgttccga tatcgacggt gacgacatcg cccggaagcg atggcgcgaa gatcacttcg
      121 cccgtgccac catcggcgcg gaaggtgttc atgaagaacg actcgccgcc gaggatgccg
      181 cgcttgaggg acttcatgag accgccggtg gcgctggtct cgatgctgac attcgcggac
      241 ttgctgagca tcgccccgcc ctcggagcgg acggactcac cctgggcgag ctcgacgcgg
      301 gcgatggcaa acgatggtcg gtagagcaat tcggtcttca caacagtctc cctgtcatcg
      361 aaaggatcag gcatacagat cgcgatcaac gggtgttgac gcgggaagag aacgcacatg
      421 atggtgactt cgtaaaatgt agcgtgcccg gaagtgggga aacaacgaag ccgacggtga
      481 tattccgacg gcttcgttcg tttttgttcg gttacgaggg ttgcggtcgg gagatcagta
      541 gtggatctcc acgcgcattc ccaccggagc gatggagtat agccagtcgg cgacatccat
      601 cggcagattg acgcagccgt ggctcatcac gttgccgaag ttgttgtgcc agtaggcgcc
      661 gtgaagggcg tggccctcat ccgtgaagta catgacgtcc ggcacgtccg gcacgttgta
      721 gtactcgccg ccgatcacgc cctccatcgt ctgtgatggg agcttgctgt tgacgaagaa
      781 ctcaccggtc ggcgtctcga agccgggtcg gccagtgctg accagcgtcg agaggatcac
      841 ctgatcgccg cagtagacgt ccatcgactg cgaggagaga ttgacatcca cccagagttc
      901 cgcgtcgacg caatcgccgc cgggtgtgct ggcaaccgga gccgggggcg ggacgaagag
      961 gtcctcgctg tacgtcgggg cgtcgccctg gtcggtcgcg gcctggttga gcttgtactt
     1021 gtccgcgagg agctgcccca cgggaacgag ccaggtgtcc ttctcctggg tccaggcgag
     1081 ctggccgcgt tcgaagacct ggtaggtgac accgttgagc gtgtattcct cggtcagggg
     1141 ttcaccgagg taactcgagc cgatgacgtt ctgccagtag tcgaggaagc catgctggac
     1201 ggcgtgcttc gtcgtctcac tgtaatagac agacccgtca tcgagattga acttcgatgc
     1261 cgacaccggg aaccacgcct ggatctggcg ggcggtatcg agggccgagg aggtcttgct
     1321 ctgctgcgag gagaaggtgc caaccgtgcg ctggacggcc atcggacgaa gctcgtcacc
     1381 gagattcgcg acgaacgccg tattgccgtc ggcatcgccc tccggccagt actcgaagcg
     1441 tgcgtactgg aggtactgca cgacgtggcc gttggactcg gtgatctcgt tcgtgatcgg
     1501 gtagccatag gcgttggcgc cgccggcgtt tcgccacatg tcgaggaaga gctgatcgag
     1561 ggtgtgaccc gtctccggaa tgtagacggt acgcggggca gaccagtcac tggatgcgga
     1621 ctgggccgcc gtgccgaacg gcatcgccat cgtcatcagg agtgatgccg cggcgatcac
     1681 ggccgcgacg agcgccctga tcgatacctt ccgcgtattc tgccgcacgc ccctcgtcgg
     1741 cgacgcaaac gccggatacg aaactgccgc ctgttccctc acccctgctc tccctttcct
     1801 gttccacgcc ggactcgcgt gtctggcatc gcgcatcgtt cccgcgcgag ccggatccgg
     1861 gcgctgccgc tgtcggatcg ccggcgcgac cagcgtcgcg atcgattccc cccggaatgc
     1921 agcgcctttc gtttaactaa acgcagctca cgagaaagag gtttcgtttc aggtatgacc
     1981 ccgcgcctcg gatcggagga tccgatagtg tacgtacact atacctcacg ctacgttcca
     2041 cgtggcaccc tgcatcgacg acgcccagac caacgtgtct ggcgtcgcgc agagctgcgc
     2101 agatgctagc gaaccctacc acgaccggga cagcgcggct cccgaatcgc gcgattgcgc
     2161 gtgaacgctt tcgaaagatc gtgaatatat gcagcagtac gcccggtatt gtgatcgcgc
     2221 ggggcgaatc
//
LOCUS       DASTMD010000098         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2508792, whole genome shotgun sequence.
ACCESSION   DASTMD010000098 DASTMD010000000
VERSION     DASTMD010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2508792"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFQ54_01535"
     CDS             <1..524
                     /locus_tag="VFQ54_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0163692.1"
                     /translation="IDGVIMRFTDILLAIPLILLLITAASLFQPSLKTTIIVIGVSSW
                     PGAARLVRGQFLQLKGLEYVTAARAIGASPVRLMARHLFPNALPIIIVETTLWLSYAI
                     LLEASLSYLGLGVQIPTPSWGNMLQEGQSELLFGAWWLTLFPGLAIFITVLAFNMLGD
                     GLRDAFDPRHRAR"
     gene            1262..1615
                     /locus_tag="VFQ54_01540"
     CDS             1262..1615
                     /locus_tag="VFQ54_01540"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0163693.1"
                     /translation="MTADQLSTVFGALADPTRRAILARLTKGDANVAELAAPFAMSQP
                     AISKHLKVLERAGLISRTRQATARLSHLEAEPLREATSWLITYREYWEESYSRLDELL
                     TTLQANQQETERTDS"
     gene            1612..2106
                     /locus_tag="VFQ54_01545"
     CDS             1612..2106
                     /locus_tag="VFQ54_01545"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU0163694.1"
                     /translation="MNTTSNNQLHLIAEPNSHAIVGIREFDAPPALVFRAFTEPELLK
                     QWLGPRRLETKVETFEVRDGGRWRFLHIDTDGTEYAFRGVFHGTPSVEGIIQTFEFEG
                     APGEVLLERMTFEDLGGRTRIHGDSISLTVAGRDAMIESGMESGMIEGYEKLDELLAR
                     LINE"
     gene            complement(2187..>2675)
                     /locus_tag="VFQ54_01550"
     CDS             complement(2187..>2675)
                     /locus_tag="VFQ54_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0163695.1"
                     /translation="EGMTDAEYLWEPATGCWSIRPDATGKWVQDRTRPEPQPTPFTTI
                     AWRMMHIADVFGRRASNQFGDGSFDLANSSLPDSVAGGLDFLRAQYENWHQGVAALDD
                     TGLARPCGPTEPYYPDNPLAGLVLHIHREFLHHAAEIHLMRDLYGHTVGRDALTLLAP
                     SR"
BASE COUNT          502 a          849 c          839 g          485 t
ORIGIN      
        1 tcatcgacgg cgtgatcatg cgcttcacgg atatcctgct cgcgatcccg ctgatcctgc
       61 tcctgattac cgcggcgtcg ctcttccaac ccagcctgaa gacgacgatc atcgtgatcg
      121 gtgtgtcatc gtggccggga gcggcgcggc tcgtgcgcgg gcaattcctg cagctgaagg
      181 ggctggaata cgtgacggcg gcgcgggcga tcggcgccag cccggtccgg ctgatggcgc
      241 gtcatctctt cccgaacgcg ttgccgatca tcatcgtcga gacgaccctg tggctctcct
      301 acgcgatcct gctggaggcg agcctgagct atctcggcct cggcgtgcag atcccgacac
      361 cgtcctgggg gaacatgctg caggagggac agagcgagct gctcttcggc gcctggtggc
      421 tgacgctctt ccccggcctc gcgatcttca tcacggtcct agccttcaac atgcttggcg
      481 atggtctccg tgacgccttc gatccccgcc accgcgcgcg atagacgctc cgtggaagga
      541 accagcggac gctggttcct gggcgaccac aagggtcgcc cctacgatca tccacgggtg
      601 gtgcctcgct gttgtccgac gcgaggattg tcgagggatc catatgcgtc ccacagcccc
      661 atcccattcg tcatcctgac cgcagggaag gacccccgtg cgaagcacag tcgagcaccg
      721 ctcgacacga tcgtcccatg catcacgctc gccgaccgcc gacgtttgat gaaccgtaga
      781 cgcgaccccg ttcggctgcg ctcagggcaa gctcttgtgg tcgtcacgcg ttggccgaag
      841 ccgatgcgcg tccatgggtg aacagcgcga tcgcataacg cgttgtacga acgtttccgt
      901 ttgcggcgag agatgaactc catcgggaga acgccgcgag tcgaaagttc cctttggcgt
      961 ccggctgcgc cggatgtgct tcgcacgggg gtccttcact ccgttcagga tgacggatac
     1021 ggggttgcgc ggaacgcatg tggatgccaa ccgatccctc gacaagctcg ggaggacggt
     1081 cgggcggttc tgcaaggcat cgttcgtggg gcgttcagat cgacctgcgg ccgataggcc
     1141 agacgagctg atccgcaacg ggctgattgg gaccctgggg ttgggccagc cgaaagaggt
     1201 tgacaaagcc agattcgatg ctaatatata accatagagt tatagaagaa agtggttatg
     1261 gatgaccgca gatcaactca gcaccgtctt cggtgcactt gccgatccga cccgccgcgc
     1321 gatcctcgcc cggctcacga aaggagatgc caatgtcgcc gaactcgctg ccccgttcgc
     1381 gatgtcccag cccgcaatct cgaagcatct gaaagtgctc gaacgggcgg ggctgatctc
     1441 gcgaaccagg caggcaaccg cccgcctcag ccacctcgag gccgagccac tccgggaggc
     1501 gacctcctgg ctgatcacct atcgcgagta ttgggaggag agctacagcc gactggatga
     1561 gctcctcacc acccttcagg caaaccagca agaaacggag aggaccgatt catgaacacg
     1621 accagcaaca accagcttca cctcattgcc gagccgaatt cgcacgccat cgtcgggatc
     1681 cgcgagtttg acgctcctcc cgcccttgtc ttccgcgcct tcaccgagcc ggaactcctc
     1741 aagcagtggc tcggaccgcg ccggctcgag acaaaagtcg agacgttcga ggtccgcgac
     1801 ggtggtcgct ggcgcttcct ccacatcgac accgacggca ccgaatatgc cttccgcggc
     1861 gtttttcacg gcacgccgtc ggtcgagggc atcatccaga ccttcgagtt cgaaggtgcg
     1921 cccggggaag tcctgttgga gcggatgacc ttcgaagatc tcggcggtcg cacccggatc
     1981 cacggggatt cgatctccct gaccgttgcg gggcgcgacg cgatgatcga atcgggtatg
     2041 gaatccggca tgatcgaggg ctatgagaag ctcgacgaac tcctcgcccg cctcatcaac
     2101 gagtagcagg cacgggcacg gtacggtacg gcacatgaag atcccggaac cacatggtcc
     2161 cgggatcttc atgtgggttc acgacatcaa cgagagggtg caagcagggt cagcgcgtcg
     2221 cgaccgacgg tatgaccgta aagatcccgc atcaggtgaa tctctgccgc atggtggaga
     2281 aattcccggt ggatgtggag cacgaggccg gcaagcgggt tgtctggata gtacggctcg
     2341 gtcggaccac acgggcgagc gaggcccgtg tcatcgagcg ccgcgacgcc ctggtgccag
     2401 ttctcgtatt gcgcccggag gaagtcgaga ccaccggcga cggaatccgg cagcgagctg
     2461 ttggcgagat cgaacgaccc gtccccgaac tggttgctcg cgcggcgtcc gaagacgtcg
     2521 gcgatgtgca tcatccgcca ggcgatcgtc gtgaacggcg tcggttgtgg ctccggtcga
     2581 gtgcgatcct gcacccactt cccggtggcg tctggacgga tcgaccagca gccggtggcg
     2641 ggttcccaga gatactcggc gtcggtcatt ccctc
//
LOCUS       DASTMD010000099         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1705015, whole genome shotgun sequence.
ACCESSION   DASTMD010000099 DASTMD010000000
VERSION     DASTMD010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705015"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..787)
                     /locus_tag="VFQ54_01555"
     CDS             complement(161..787)
                     /locus_tag="VFQ54_01555"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HEU0163696.1"
                     /translation="MAAVATSWTVLIYRVPTEPASGRVSIWRDLKQMGALYLQQCACI
                     LPAREDLTPKVTRVIGKIADLGGEHWLLAIPTVPEADEARLTSSFRELRNKEYAEIIE
                     ECETKFVKEIEFEHFRKNYSFEEAEEIGQDLEKIRRWFRRVTERDWFDADRRPEVEAW
                     IDRCAEMLAQFEEVVYRQAGDDFAPATPPLAIPNLAPDEPDAKTEEIQ"
     gene            1214..>2325
                     /locus_tag="VFQ54_01560"
     CDS             1214..>2325
                     /locus_tag="VFQ54_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0163697.1"
                     /translation="MQQSQPPWRNSRQKALSRRAALGAGVALVAGLGGVAWALDSNDK
                     LPGTSPTPTPAPTSTPPPPTATTIPTPTPTATRAPASPVSSPAASPAAIAAADLLVEA
                     KLSDLRAAIDNGSVTIVELVQATLDRVTAMDGNDTDTVLHAVIETNPDALAIAKTLDE
                     ELQAGESRGPLHGIPVMVKDVFASGDQMKTTAGALALAENTVVEDSFLVARLRDAGMV
                     LMGKANMSEWSAFRSSGLGSGWSGRGGQASNPYVLDRSPWGSSAGSAIAVASSYVPFA
                     LGAETDGSILCPSSACAVVGLKPTVGLTSRVGVIPISSSQDSPGAIARNVYDLAVFFS
                     AIAGYDAEDSAYNEFSGSAPASEFSTWPVPDVGSVD"
BASE COUNT          384 a          772 c          734 g          435 t
ORIGIN      
        1 gcgccgacaa tgatcgccag cacgacgagc gcgacggcgg tgccgatgag tggcgatcgc
       61 cagccacggg tgacgcccat cgcgaagatg atcgtcagcg cctccaccat ttcaacggtg
      121 ctcgcgagaa aggctcccag cacgagcagg atgtctgtgg tcactggatt tcctccgttt
      181 tggcatcggg ttcgtccggg gcaaggtttg gtatcgccag gggcggcgtc gcaggtgcga
      241 agtcgtcgcc cgcctggcgg tagacgacct cctcgaactg ggcgagcatc tcggcgcagc
      301 gatcgatcca tgcttcgact tccggtcgtc gatcggcatc gaaccagtca cgctcggtga
      361 cccgccggaa ccagcggcgg atcttctcga ggtcctgccc gatttcctcg gcctcctcga
      421 acgaatagtt cttccggaaa tgctcgaatt cgatctcctt tacgaacttc gtctcgcact
      481 cctcgatgat ctcggcgtac tccttgttcc tcaactcgcg aaagctggac gtcagcctcg
      541 cttcgtcggc ctccgggacc gtagggatcg cgagcagcca atgctcgccg cccagatcgg
      601 cgatcttgcc gatgacccgc gtgactttcg gggtgagatc ctccctcgcc gggaggatgc
      661 aggcgcactg ctggagatag agcgcgccca tctgcttgag gtcgcgccag atcgagaccc
      721 ggccgctcgc cggctcggtg ggcacccgat agatcagaac ggtccaactc gttgcgactg
      781 ccgccacatt gcttccttcg cgcgactgct acagattcag cgatgttaca cctgtacatc
      841 atggagtgat cgggatcgaa acgcaaggcg tcacgggcgt gatcatgtgg atgagccggt
      901 ccagaaccgg cgtgaacata cctggaaggt gatgtccggc tttctccaag gtgcgcccgc
      961 tacgcttcag ggagagacgg gcgttgacgc acggctccgg ggacgatcgg ttgaacctcc
     1021 tggcggagtg cactatgctc cctgccaaca tggaagccgt acgtacatgc ggctcccacc
     1081 cgctctccgt ttccggggaa ccagtctggc tgggctgagt cctgatcgga gacttccacc
     1141 acgcatctca ggtcgtttgc cgccccgttg agggtgggcg gtacgcgtgc ctggtcagga
     1201 tcccgcttct cgcatgcagc aatcgcaacc cccctggaga aactcgcgac agaaggcact
     1261 ctctcgtcgc gccgcgcttg gtgccggcgt cgcccttgtc gccggtctcg gtggtgtggc
     1321 ctgggcgctc gacagcaatg acaagttgcc cggcacgagt cccacgccga caccggcacc
     1381 gacctccaca ccaccgccgc caaccgccac gaccatcccc accccgactc cgaccgctac
     1441 ccgggcgccg gcgtctcccg tctcttcgcc ggcagcgagt ccggcggcga tcgcggcagc
     1501 cgatcttctc gtcgaggcga agctctcgga tctccgcgcg gcgatcgaca acggctcggt
     1561 cacgattgtc gagctcgtgc aggcgaccct cgatcgggtc accgcgatgg atggcaacga
     1621 taccgacacg gtgctccacg ccgtgatcga aacgaacccg gatgcgctcg cgatcgcgaa
     1681 gacccttgat gaggaactac aggccgggga atcccgcggg ccacttcatg gcattcccgt
     1741 gatggtgaag gacgtcttcg cgtccggcga tcagatgaag acgaccgccg gcgccctcgc
     1801 cctcgccgaa aacaccgtcg tcgaggattc cttcctcgtc gcccgcctgc gtgatgccgg
     1861 gatggtgctg atgggcaagg cgaacatgag cgagtggtca gccttccgtt cgtccggtct
     1921 cggaagcggc tggagcggtc gtggcggaca ggcgagcaat ccctacgtgc tggaccgatc
     1981 gccgtgggga tcgagcgcgg gctcggcgat cgccgtggca tcgagctacg ttccgttcgc
     2041 gctcggcgcc gagacggatg ggtcgatcct ctgcccgtcg tccgcctgcg ccgtcgtcgg
     2101 gctgaagccg accgtggggc tgacgagccg ggttggtgtg atcccgatct cgagttcgca
     2161 ggattcgcct ggtgcgatcg cccggaacgt ctacgatctc gcggtcttct tctccgcgat
     2221 cgccgggtac gacgcggagg attccgccta caacgaattc tcgggaagcg ccccggcgtc
     2281 ggaattctcg acgtggcctg tccctgacgt cgggtcggtg gatta
//
LOCUS       DASTMD010000100         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_634800, whole genome shotgun sequence.
ACCESSION   DASTMD010000100 DASTMD010000000
VERSION     DASTMD010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634800"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1197)
                     /locus_tag="VFQ54_01565"
     CDS             complement(130..1197)
                     /locus_tag="VFQ54_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0163698.1"
                     /translation="MEQLGLEDIETGIGGATGVTALIRGKKSGSDKVVLVRADMDALP
                     ILEENAVEYVSQNPGVMHACGHDAHTAMLLALSRVLMDRRDEFSGTVKVLFQPAEELP
                     PGGAIGMVKAGVLENPHVDAVLGMHVSSQLEAGKIGLRPAGPGSAAGDRFRITIQGKG
                     GHAARPQVAVDPVVIGSQIVLALQTLVARETDPMASAVVSTTAFLAGEAFNVIPDTAE
                     LRGTVRTISPEVREHMAKWLPEIAKGIGEAGRADVTVDWRPGYPSVINDEAMIAFVEA
                     AAIEVVGEENVVQSPLGMGGEDFSYFSIERPGCFFNVGTRSEERGITWGHHHPRFDID
                     EEGLSAGIGTMTNAVLHFLAS"
     gene            complement(1424..>2589)
                     /locus_tag="VFQ54_01570"
     CDS             complement(1424..>2589)
                     /locus_tag="VFQ54_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0163699.1"
                     /translation="IMPGVIADRRWMHEHPELGFEEFETAKFVADRLRQLGVEDIQTG
                     IDGATGITGIIRGTKPGSDKVVLVRADMDALPILEENAVEYVSQNPGVMHACGHDAHT
                     SMNLALARILMSRRDEFSGTVKVLFQPAEELPPGGAIGMIRAGVLENPHVDAVLGLHV
                     SSQVPAGKIGIKAPGPGCAAADRFRITIQGKGGHAARPQVAVDPVVIGSQIVLALQTI
                     VSRETDPMDNAVVSTTAFIAGEAFNVIPDTAELRGTVRTLNEDVRQNISRRLPEIAKG
                     IGEAGRAEVTVDWMWGYPSMILHESMVGFVREGAIAAVGEENVVDMGVQLAGEDFSYF
                     TLERPGYFFNVGTRNEERGITWGHHHPRFDLEEEGMAAGIGTMVNTVLAYLAS"
BASE COUNT          428 a          858 c          856 g          447 t
ORIGIN      
        1 caggctcgga atgacgttca tgcggaggtg tcgtgcgggc gggcgcggag gtcgaccggc
       61 ggcgagggaa cacgagacgg ggccaggatt actcccggtc ccgtctcatg tctgtatctg
      121 tgcgagggtc tacgacgcga ggaagtggag gacggcgttg gtcatcgtgc cgatgcccgc
      181 gctgagacct tcctcgtcga tatcgaaccg cggatgatgg tgaccccagg tgatgccacg
      241 ctcttcgctg cgcgtgccga cgttgaagaa gcatcccggg cgctcgatcg agaagtagga
      301 gaaatcctcg ccacccattc ccaacgggga ctggacgacg ttctcctcgc cgacgacctc
      361 gatcgccgcc gcctcgacga aggcaatcat cgcctcgtcg ttgatcacgc tcgggtagcc
      421 cgggcgccaa tcgacggtca cgtcggcgcg accggcttcg ccgattccct tcgcgatctc
      481 cggtagccac ttcgccatgt gctcgcggac ttccgggctg atcgtgcgca ccgtgccacg
      541 cagttccgcc gtgtccggga tcacgttgaa tgcctcgccg gcgaggaacg cggtcgtcga
      601 gacgacggcg ctcgccatcg gatccgtctc acgcgcgacc agggtctgca gcgcgaggac
      661 gatctgcgat ccgatgacga ccggatcgac ggcgacctgg gggcgtgccg catgaccgcc
      721 cttgccctgg atcgtgatcc ggaagcgatc gccggcggca gatcccggac ccgccggacg
      781 gaggccgatc ttgccggcct cgagctggga cgagacgtgc atgccgagca cggcgtcgac
      841 gtgcgggttt tcgagcacgc ccgccttcac catcccgatc gcgccaccgg gcggcagttc
      901 ctccgccggc tggaagagca ccttcaccgt gccggagaac tcgtcgcggc gatccatgag
      961 gacgcgtgag agggcaagca gcatggccgt gtgggcatca tgtccgcagg catgcatcac
     1021 gccggggttc tgggagacgt actcgacggc gttctcctcg agaatcggca acgcatccat
     1081 gtcggcacgg acgagcacta ccttgtcact gccgctcttc ttgccgcgaa tcagggcggt
     1141 cacaccggtc gcgccaccga tcccggtctc gatatcctcg agtccgagtt gttccagtcg
     1201 cgctgcgacg aacttcgcgg tctcgaactc atggaaaccg agttaggggt gctcatggag
     1261 ccaccggcgg tcggcaacga cgcccggcat gatctcgtcg atgtctgtac gtaatgtcgt
     1321 agccgtgacc atcggtaccc tcctcgaagt gtcgatgcgc gatacaggag tgcgcagtgt
     1381 caatgagtat ggcgcgacct cgtgtcgcgc ccgttgatcg aacctacgac gcgaggtagg
     1441 cgaggacggt gttgaccatc gtgccgatcc cggccgccat cccctcttcc tcgagatcga
     1501 agcgcgggtg gtggtggccc caggtgatgc cacgctcctc gttccgggtg ccgacgttga
     1561 agaagtagcc cgggcgctcg agggtgaagt aggagaaatc ctcgcccgcc agctgcacgc
     1621 ccatgtcgac gacgttctcc tcgcccactg cggcaatcgc gccctcgcgg acgaagccga
     1681 ccatcgactc gtggaggatc atgctcgggt acccccacat ccagtcgacg gtgacctccg
     1741 cgcggccagc ctcgccgatg cccttcgcga tctccggcag ccgcctcgag atattctggc
     1801 ggacgtcctc gttcagcgtg cgcacggtcc cgcgcagttc cgccgtatcc gggatcacgt
     1861 tgaaggcctc cccggcgatg aacgcggtcg tcgagacgac ggcgttgtcc atcggatcgg
     1921 tctcgcggga gacgatcgtc tgcaacgcga gaacgatctg ggaaccgatg acgacgggat
     1981 cgaccgcgac ctgcgggcgg gcggcgtgac cgcccttgcc ctggatcgtg atccggaagc
     2041 gatcggccgc ggcgcagccc ggacccggcg ccttgatccc gatcttcccg gccggaacct
     2101 gggatgagac gtggagaccg agaacggcgt cgacgtgggg attctcgagc accccggccc
     2161 ggatcatccc gatcgcgccg cccggcggga gttcctccgc cggctggaac agcaccttca
     2221 ccgtgccgga gaattcatcg cggcgcgaca tcaggattcg ggcgagcgcg agattcatcg
     2281 aggtgtgggc gtcgtggccg cacgcatgca tcacgccggg attctgggaa acatactcga
     2341 cggcgttttc ctcgaggatc ggcagcgcgt ccatgtccgc gcgaacgagg accaccttgt
     2401 cgctgccggg cttcgtgccg cggatgatgc cggtgatacc ggtcgcgccg tcgatgccgg
     2461 tctggatgtc ctcgacgccc agctggcgca ggcggtcggc gacgaacttc gcggtctcga
     2521 actcctcgaa accgagctcg ggatgctcgt gcatccagcg gcgatcggcg atcacgccag
     2581 gcatgatct
//
LOCUS       DASTMD010000101         4475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1973399, whole genome shotgun sequence.
ACCESSION   DASTMD010000101 DASTMD010000000
VERSION     DASTMD010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973399"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VFQ54_01575"
     CDS             complement(<1..665)
                     /locus_tag="VFQ54_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163700.1"
                     /translation="MVTATFAEVGIDTKGRVSGQVKTKCPRCDHDRKPAHRHDRPLSV
                     NIDDGVWLCHNCGFTGSLKPEDWQTPIKPERHYDRPQIAAERRLGERARAFLAERAID
                     PELAIELGVYSNPEDTAIAFPYVRDGEVVHVKYRRIREKAFWSTQNTELVMYGLDECR
                     DADTVVICEGELDRLALMTAGITTVMSVPNGAIQPGANPGAKLDCLHSAEQIIANAKR
                     VVIA"
     gene            complement(659..1507)
                     /locus_tag="VFQ54_01580"
     CDS             complement(659..1507)
                     /locus_tag="VFQ54_01580"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0163701.1"
                     /translation="MGALRMIHDEDVPRYDGQAVPRQTRFAMIPQAVRSLRDPYAKAV
                     YMTLAFYADSDGKCFPSRQTIADETGCSMRKVDNVLATLEDAGLIVIETSDKAIKRAR
                     MIHLVRNPPVRNNPETTHLHEVHITEEIPEESHMQEVQVEEPDVHQVRADVQEVRVDV
                     HHVPNDVQEVHPNKTQEQDPTNKTQEQERSTALAPADGFDAFWLAYPKHAEKKQAVDQ
                     WRRLKPSLELQATILAAIVAQKSSRKWREGYIKAPHRWLRDQNWQDEVEPVTTSGKGP
                     LGLASW"
     gene            complement(1548..1925)
                     /locus_tag="VFQ54_01585"
     CDS             complement(1548..1925)
                     /locus_tag="VFQ54_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU0163702.1"
                     /translation="MASFARIAVFGNLGSDPETKYTPSGAMVVSFSIAVNPRRKGANG
                     AEEKPVWYRVSAWDRLAERIDRLAQQQYIAKGRQLYVEGSFEPREFSGNDGSTRISYD
                     ITMTDFQFVGGGDQSSSKEEAPF"
     gene            complement(1939..2271)
                     /locus_tag="VFQ54_01590"
     CDS             complement(1939..2271)
                     /locus_tag="VFQ54_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163703.1"
                     /translation="MSGSSLVEAIARAGFEAAEREIEYRAKQSGGYRHARIWEEASEL
                     DRIWWRYVVDKGAQSPQDIFERRMEGAYGVKPWMKKSDRERIVWERISNAMNAARRRA
                     SIDAMAAD"
     gene            complement(2268..2948)
                     /locus_tag="VFQ54_01595"
     CDS             complement(2268..2948)
                     /locus_tag="VFQ54_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163704.1"
                     /translation="MAEITRQAFNPNEHLMKLKGKDYLEVKWRLVWFRQEYPHGRITT
                     AIHQITDTHAIVTAQVDDGVGGTGSGIGSESVKDFGDFIEKAETKAIGRALAALGFGT
                     QFAPELEEGERIVDSPVARPQSTPARPRTESPAPKATDQQRNFIIGLGKQLGMVKADG
                     HHDGEKLDQALSAAVGTTLADLSVPQAKEAIDRMKKKVAALPKATGPAPVADLDGNIS
                     HATRHMNA"
     gene            complement(3001..3288)
                     /locus_tag="VFQ54_01600"
     CDS             complement(3001..3288)
                     /locus_tag="VFQ54_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163705.1"
                     /translation="MQQLYRTSKPTRTYRPVQKPARDAGERAVRSAIAALEEEVEMVY
                     DQLYDAMRSRKSKTSHHHLDHLLALGDRYDELQADLQAMAGEGSQANPVAA"
     gene            complement(3341..3703)
                     /locus_tag="VFQ54_01605"
     CDS             complement(3341..3703)
                     /locus_tag="VFQ54_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163706.1"
                     /translation="MAATTRIIQLQNRFDAFVERVEERDDYVAEIVADHVVTAEEWEG
                     YRARQRVIEADTEDIRNEMSIVSDAHTLAQVVLNAGAVTPWVERQTRTRAQDYLRLVE
                     RPQNIVDFPDGTEPQRAA"
     gene            complement(4152..>4475)
                     /locus_tag="VFQ54_01610"
     CDS             complement(4152..>4475)
                     /locus_tag="VFQ54_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163707.1"
                     /translation="TIAVYRKTPTSNDLWLPVFKGNDLTKARTALDLAANRADTTEMQ
                     TIGVWGRWGADDYTDIRSDISADGEVVYKIRNAQRSDAEHRPIYQTLVLPDVLPVIAV
                     AAKAA"
BASE COUNT          713 a         1381 c         1408 g          973 t
ORIGIN      
        1 gcgatcacaa cgcgcttcgc gttggcgatg atctgctcgg ccgagtggag acaatcgagc
       61 ttggcccccg gattggcgcc gggctgaatc gcgccattcg gaaccgacat gacggtcgtg
      121 atccctgccg tcatcaatgc caggcgatcg agctcgccct cgcagatcac gaccgtgtcg
      181 gcatcacggc actcgtctag cccgtacatc accaattcgg tattctgggt cgaccagaac
      241 gctttctccc ggatgcgacg gtacttcaca tgcacaacct cgccgtcacg aacgtacggg
      301 aacgcgatcg ctgtgtcctc gggattcgaa tagacgccga gttcgatcgc gagttcggga
      361 tcgatggcgc gttcggcgag aaaggcgcga gcccgctcac cgagccgacg ttctgccgcg
      421 atctgcgggc gatcgtagtg ccgctccggc ttgatcggcg tctgccaatc ctccggcttg
      481 aggcttccgg tgaatccgca gttgtggcag agccagacgc cgtcgtcgat gttcacggag
      541 agcgggcgat cgtggcgatg cgccggcttg cggtcgtgat cgcaccgtgg gcacttcgtc
      601 ttgacctgtc cgctgacgcg gcccttcgtg tcgataccca cctcggcaaa ggttgcagtt
      661 accatgacgc caaccccaac ggtcccttac cggacgtcgt cacaggctcg acttcgtctt
      721 gccagttctg gtcgcggagc cagcgatgcg gtgccttgat gtaaccctct cgccatttgc
      781 gggacgactt ctgcgccacg atcgccgcga ggatcgttgc ctggagttcg agagacggtt
      841 tgagccggcg ccactggtcg accgcctgtt tcttctcggc gtgtttcggg tacgcaagcc
      901 agaacgcgtc gaatccgtcc gcaggtgcca acgcggtaga tctctcttgt tcttgggttt
      961 tgttcgttgg gtcttgttct tgggtcttgt tagggtgcac ctcctgcaca tcattcggca
     1021 cgtggtgcac atcaacacgc acctcctgca catcagcgcg cacctggtgc acatcaggtt
     1081 cctctacctg cacctcctgc atatgcgatt cttcggggat ttcctccgtg atgtgcacct
     1141 cgtgcagatg cgtcgtttca gggttatttc gcacgggagg gttcctgacg agatggatca
     1201 tccgggcacg cttgatggct ttgtcgctcg tttcgatgac gatcaggccg gcgtcctcga
     1261 gcgtggcgag gacgttgtcg accttgcgca tggagcaccc ggtctcgtcg gcgatcgtct
     1321 ggcgggacgg aaagcacttc ccgtcactgt ccgcatagaa cgcgagtgtc atatagaccg
     1381 ccttggcata gggatcgcga agactgcgga cggcctgggg gatcatggcg aaccgggtct
     1441 ggcgaggcac agcttgcccg tcatagcggg gcacgtcctc gtcgtgaatc atgcgaagtg
     1501 cgcccatgtg tccccccagg tgtcagtcgt cgtcgcgatc gtcggcatta gaacggcgcc
     1561 tcttccttgg acgacgactg atcgccacca cctacgaact ggaagtccgt catcgtgatg
     1621 tcgtaggaga tccgcgtcga tccatcgttt ccggagaact cccgcggctc gaacgatccc
     1681 tcgacgtaga gttgtcttcc cttggcgatg tactgctgtt gggcaagacg atcgatccgc
     1741 tcggcgaggc gatcccatgc gctcacgcga taccagaccg gcttttcctc ggcaccgttg
     1801 gctcccttgc gacggggatt cacggcgatg ctgaaggaca cgaccatcgc cccgctcggc
     1861 gtgtacttgg tctccggatc acttccgagg tttccaaaga cggcgattct ggcgaaactt
     1921 gccattggtg ttctgctcct agtccgcggc cattgcatca atagatgcac ggcgccgtgc
     1981 ggcattcatc gcgttggaga tccgttccca gactatgcgt tcccggtcgc tcttcttcat
     2041 ccagggcttg acgccgtagg cgccttccat gcgtcgctcg aagatgtcct gcgggctctg
     2101 ggcacccttg tcgacgacgt agcgccacca gatccgatca agctcgctgg cctcttccca
     2161 gatgcgagcg tgtcgatagc caccgctctg cttggcgcga tattcaatct cgcgttccgc
     2221 agcttcgaaa cctgcgcgcg cgatggcctc gaccagagac gagccgctca tgcgttcatg
     2281 tgccttgtgg cgtgtgagat gttgccgtcc agatcagcca ccggagccgg acctgtggct
     2341 tttgggagcg cggcgacctt cttcttcatg cggtcgatcg cttccttcgc ctggggaact
     2401 gagaggtcgg cgagcgtcgt ccccacggct gccgatagtg cctggtcgag cttttccccg
     2461 tcatgatggc cgtccgcctt caccatcccg agttgcttgc cgagcccgat gatgaagttg
     2521 cgctgttggt ctgttgcctt gggcgccgga gattcggtac gaggtcgtgc cggagttgat
     2581 tgcggccgcg ccacggggga atcgacgatc cgctccccct cctccagctc gggggcaaat
     2641 tgggttccga aaccgagggc ggcaagcgcg cgcccgattg ccttcgtctc cgccttctcg
     2701 atgaaatcgc caaaatcctt cacggactcg gagccgatgc cggaaccggt tccgccgacg
     2761 ccgtcgtcca cctgggcagt gacgattgcg tgcgtatccg tgatctgatg gatcgccgtc
     2821 gtgatccggc cgtgcgggta ttcctgccgg aaccatacga ggcgccattt cacctcgagg
     2881 tagtccttgc ccttgagctt catcagatgt tcattggggt tgaaggcttg tcgtgtgatc
     2941 tctgccatcg tgattcctca acaggaacgg gcggccatgc gaccgcccgc gatccggtgt
     3001 ttacgctgca acagggttcg cttgagatcc ctcgccggcc atcgcctgca gatccgcctg
     3061 gagttcgtcg tagcgatcgc cgagcgcaag caaatgatcg aggtggtgat gtgacgtctt
     3121 ggacttgcgg gaccgcatgg cgtcgtagag ctggtcgtag accatctcga cttcttcttc
     3181 gagcgcggcg atcgcgctgc gcactgcgcg ttcgccagcg tcccgcgccg gcttctgtac
     3241 tggacgatag gttcgcgttg gcttggatgt gcggtacaat tgctgcattg gtatttctcc
     3301 ttggagcgct ttcacccttg cccggtggag gcgcttttcg ttaggccgcg cgctgcggct
     3361 cggtcccgtc cgggaagtcg acgatgttct gcggtcgctc cacgagccgg agatagtcct
     3421 gcgcccgcgt cctcgtctgc cgttcgaccc agggcgttac cgcccctgcg ttgagcacga
     3481 cctgcgcgag cgtgtgtgca tccgagacga tcgacatctc gttgcggata tcctccgtgt
     3541 cggcctcgat cacgcgttgc cgcgcgcgat acccctccca ttcctccgcc gtcacgacgt
     3601 gatcggccac gatctcggcg acgtagtcgt cgcgctcttc gacacgctcg acgaacgcgt
     3661 cgaaccggtt ctgcaactgg atgattcggg ttgttgctgc cactgcgggg accattcctg
     3721 atgggtacat acgccggtgc tttgccggca ctacagtaag aagacggacc gggacggttg
     3781 ttttgtccgt acgccgaagc tgcacgaccg tcccgtaccg ctgaacggct gcaggcatgt
     3841 cgctcaacga cgcccgcttg agttgtgggg tgctcgccat tacagcaagc ggcgcatagg
     3901 tcactccttt cgtgtgactc aggccggatg gatagcccga gggtctgacc ttgagcaggg
     3961 aggggcatgt cgcgacctgg ggggactcag cgacaagtgg ggagaacctg ctcaaggtca
     4021 gaccctccgg agaccggagg agctgagcgg tcgcggctga ggaagccggg agttcgaccg
     4081 cggggaaccg acgaagacgt gacggggacg gagccgcttg ggagcgggcg gagagaacgg
     4141 tgggattcat atcaggcggc cttcgccgcg acggcgatga ccggcagaac gtcgggcagc
     4201 acaagcgtct ggtagatcgg gcgatgttcg gcgtcggagc gctgggcgtt gcggatcttg
     4261 tagacgacct cgccgtccgc gctgatgtcg gaccggatgt cggtatagtc gtccgcgccc
     4321 cagcgacccc agacgccgat cgtctgcatc tccgtggtgt cggcacgatt cgccgcgaga
     4381 tcgagggccg tgcgcgcctt ggtgagatcg ttgcccttga agaccgggag ccagagatcg
     4441 ttgctcgttg gcgtcttgcg gtagacggcg atcgt
//
LOCUS       DASTMD010000102         1985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2151791, whole genome shotgun sequence.
ACCESSION   DASTMD010000102 DASTMD010000000
VERSION     DASTMD010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151791"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VFQ54_01615"
     CDS             <1..458
                     /locus_tag="VFQ54_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163708.1"
                     /translation="REWASAPDAPVVVVEFADHSGDETTEQFVSELRRLASDLEFPLD
                     DDNLLIVLAAARWVVPDRHDFEQAVHALATIAIVKPDGPLNLDEAMIVSDDLDDGVEK
                     AVERAPEVISETRKLIQTWIQANGGRYRRLGTALQAVALRLGGVAGSRV"
     gene            complement(805..1332)
                     /locus_tag="VFQ54_01620"
     CDS             complement(805..1332)
                     /locus_tag="VFQ54_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163709.1"
                     /translation="MNTTDTIVTAMWTGILAALVASLVWFLIFRLIRPRIRIAPIGTG
                     DPATGWIRIKVLNGSRRDLIDLRFELDVMRPRVSPRGVTYGRSRVSLAGPAPVIIAGR
                     GRPDDSNAYRVAAQLDPTDVFTKDRAYFVRFRVFGRDALSGVGRVIEMRYDTLSTFVE
                     GEYEKGQTFQIVRDV"
     gene            complement(1329..1571)
                     /locus_tag="VFQ54_01625"
     CDS             complement(1329..1571)
                     /locus_tag="VFQ54_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163710.1"
                     /translation="MDVDVAWPFRLSFPLTRLLGLVLHDEEEEEEDGGYWVPARVKDK
                     GASARPTRFSPSASTTREPDAADPAPGLHVDIEVEE"
     gene            complement(1640..>1985)
                     /locus_tag="VFQ54_01630"
     CDS             complement(1640..>1985)
                     /locus_tag="VFQ54_01630"
                     /inference="COORDINATES: protein motif:HMM:NF028211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0163711.1"
                     /translation="PARAAVLFERYGTVAREVAAFLAEGPDAPVVGERLSTRELTWMV
                     ERERVVHLNDVLLRRTDLGFTGALGDDVVEAVATALADAAGWDAARRDAELARAREEL
                     AARHGILAPATR"
BASE COUNT          336 a          643 c          651 g          355 t
ORIGIN      
        1 ctcgcgagtg ggcttccgcc cctgacgcac ccgtagtggt cgtcgaattc gcagatcact
       61 ctggcgacga gacgaccgag caattcgtct cagaactacg gaggttggct tccgacctgg
      121 agttcccgct cgacgacgac aacctactga tcgtccttgc ggcggcgcga tgggtcgttc
      181 ccgatcggca cgacttcgag caagcggttc acgccctcgc cactattgcg atcgtgaagc
      241 ccgatggccc tttgaatttg gacgaggcga tgatcgtttc ggacgacctc gatgacggcg
      301 tggagaaggc ggttgaacga gcgccagagg tgatttctga aacccgtaaa ttgatccaaa
      361 cttggattca ggctaacggt ggtcgctaca ggcgcctcgg aactgctttg caggcggtcg
      421 cccttcggct tggtggcgtg gctggttctc gtgtttaggc gcgtggcgag cggactcagc
      481 cttagctggg ttaaagcatg gagcgccgag agcctcctcg tggccgaatc ctggcaccgg
      541 cttgcttcag gacggctagg actgcggtct tcgagaccgt attcctcacg gccacagcgc
      601 gcgtgctcag acccgattcg tagtcttcaa cgatcttgcg ccggcggatc atcgtgagtc
      661 gagcaacgat cggaggccga gatcgtcgtt ttaccgggga tttgcttacg acaagggtat
      721 tgctcttcag tgaggacagg accatcaagt cgagggcccg gtcgtccgac ccggcccttc
      781 ttcgtgtgcg atgaagaacg tggtttagac atcccggacg atctggaacg tctggccctt
      841 ctcgtactct ccctcgacga aggtcgagag ggtgtcgtaa cgcatctcga tcacacggcc
      901 gacaccgctc agggcatctc ggccgaatac ccggaaacgc acgaagtatg cccggtcctt
      961 cgtgaacacg tcggtgggat ccagctgcgc cgcgacccgg tacgcattcg agtcatccgg
     1021 gcgtcctcgt cctgcgatga tcaccggagc cgggccggcg agcgagacgc ggctcctccc
     1081 gtatgtcacg ccacgcgggc tgactcgcgg acgcatcacg tcgagctcga aacggagatc
     1141 gatcaggtcg cgtcggctgc cattgaggac cttgatccga atccacccgg tcgcgggatc
     1201 cccggtgcca atcggagcga tccggatgcg gggacggatc agtcggaaga tcaggaacca
     1261 gacgagcgac gccacgaggg cggcgaggat gccggtccac atcgccgtga cgatcgtgtc
     1321 agtggtgttc actcctcgac ctcgatgtcg acgtgcaacc cgggtgcggg gtcagcggcg
     1381 tcgggctctc ttgtcgtcga ggcggagggg ctgaagcgcg tcggccgagc gctcgcaccc
     1441 ttgtccttca ctctcgccgg cacccagtag ccgccgtcct cctcctcttc ttcctcatcg
     1501 tggagaacga gacccagaag tcgcgtcagc gggaacgaga gtcggaaagg ccaggcgacg
     1561 tcgacgtcca tggagatctt gctaccagac ctcgtcgctg attcggagga ggtcgggacg
     1621 atcctcgccg ctcgcgttct cagcgcgtcg cgggcgcgag gatcccgtgc cgcgccgcga
     1681 gctcctcgcg ggcccgcgcg agctccgcgt cgcgccgcgc cgcgtcccat ccagccgcgt
     1741 ccgcgagggc ggtcgcgacc gcctcgacca cgtcgtcgcc gagggcgccc gtgaagccga
     1801 ggtcggtgcg ccggaggagc acgtcgttca gatgcacgac ccgctcccgc tcgaccatcc
     1861 acgtgagctc gcgcgtcgaa aggcgctccc cgaccaccgg agcgtcgggg ccctccgcga
     1921 ggaacgccgc gacctcgcgg gcgaccgtgc cgtagcgctc gaagaggacc gcggcgcggg
     1981 ccggg
//
LOCUS       DASTMD010000103         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1795735, whole genome shotgun sequence.
ACCESSION   DASTMD010000103 DASTMD010000000
VERSION     DASTMD010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795735"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VFQ54_01635"
     CDS             <1..1360
                     /locus_tag="VFQ54_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU0163712.1"
                     /translation="ALLGLFVIGIPFIGFLGLGAAVAVGMSVLIALGLMPALLRLLGG
                     PLDRFAFVPNRQRPVESTFGFKWVSRIQKLPAVWLVGALIVLGGLGSVALFGAQLGSS
                     DAGTNPADSTTRKAYDLISQGFGPGANGPLLVVIENANGGKVDQASLDKLAVAFGSTS
                     GVVAKPVAVPNPAGDTAIMTVIPTTSPQDTKTEDLIKDLRKNVIPPVADPNGLHVYIG
                     GATATFADLSSTTSSRLPIFIALVAGLSVILLMAVFRSVLIPIKAAVVNLLSFFAGYG
                     VLVAIFQKGFLGINDWLFGVDKLGPIESFLPVFLFSILFGLSMDYEIFLVSRVHEAWG
                     HYHDNGKALRNGIGASGRVVLSAGAIMTCVFFAFTLGSQRSIKEIGLGLGSAILIDVL
                     IVRMIVVPSFMSLAGNANWWFPAWLDRILPNLNVEGDTASTDHSDPTDGTTPKPVIAT
                     QS"
     gene            1658..2629
                     /locus_tag="VFQ54_01640"
     CDS             1658..2629
                     /locus_tag="VFQ54_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163713.1"
                     /translation="MVAATPSGGIHVVAAVDIGSNTIKMSIARQAEANANGFEELASG
                     SHTVRLGAGLAETGRLADDRIASALETLTAFAGEARRHGATRLVGVATAATRQAANGP
                     EFLASVANETGWDVRVVSGDEEAALTFRGLASEHDLSGIVVVADIGGGSTEVIVAEHE
                     VIRSSQSLPLGSGALTERFVVSDPPATAELDACATAAIDALTVIDWPAAGGRAHLIVV
                     GGTAEAVAKLLPPKAPVTRTAIEQAIATCRDLSSTELSDRFELPPARARVLPAGLTII
                     AELARMVAPDRISLARSAIRSGLLLATFDDLRAQTSAPTSPSSSGGD"
     gene            2654..>2997
                     /locus_tag="VFQ54_01645"
     CDS             2654..>2997
                     /locus_tag="VFQ54_01645"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEU0163714.1"
                     /translation="MTKTPAPERENHDATYKAAMRQAIAARWADTWRELAKVAKSEDP
                     EAVHATRVASRRLRAAMDAATDAFPRKWYRKLHKMAKTITSELGTVRDTDILLQELQA
                     ERARADASEWVGL"
BASE COUNT          483 a          968 c          978 g          568 t
ORIGIN      
        1 cgcgctcctc ggtctcttcg tgatcggtat tccgttcatc ggcttcctcg gtcttggtgc
       61 tgcggtggcc gtgggtatga gcgtgttgat cgcacttggc ctgatgccgg cgctgctgcg
      121 cctgctcggt ggcccgctcg accgcttcgc cttcgtgccg aatcgtcagc gcccggttga
      181 gagcaccttc ggcttcaagt gggtgagccg catccagaag ctgccggccg tctggctcgt
      241 cggcgcgctc atcgttcttg gcgggctcgg ctccgtcgcg ctcttcggcg cccagctcgg
      301 ttcgtccgat gccggcacca acccggctga ttcaacgacc cgcaaggcct acgacctgat
      361 ctcgcagggc ttcggcccgg gtgccaacgg tccgctcctg gtcgtgatcg agaacgcgaa
      421 cggcggaaag gtcgaccagg catcgctcga caagctcgct gttgccttcg gttcgacctc
      481 tggcgtggtc gcgaagccgg ttgccgtgcc aaatccggct ggtgacacgg cgatcatgac
      541 cgtgatcccg accacgtcgc cgcaggacac gaagacggaa gacctgatca aggacctccg
      601 gaagaacgtg atcccgccgg tcgcggaccc gaacgggttg catgtctaca tcggcggcgc
      661 gaccgcgacc ttcgccgacc tctcgtcgac gacctccagc cggttgccga tcttcatcgc
      721 actcgtcgcc ggtctcagcg tgatcctgct gatggcggtc ttccgttcgg tcctgatccc
      781 gatcaaggcc gcggtcgtga acctgctctc cttcttcgcc ggctacgggg tgctggtggc
      841 gatcttccag aagggcttcc tcggcatcaa cgactggctc ttcggggtcg acaagctcgg
      901 gccgatcgaa tccttcctgc cggtcttcct cttctcgatc ctcttcgggc tctcgatgga
      961 ctacgagatc ttcctcgtca gccgcgtcca tgaggcctgg gggcactacc acgacaatgg
     1021 gaaggcgctc cggaatggca tcggcgcctc gggtcgcgtg gtgctgagcg caggcgcgat
     1081 catgacgtgc gtcttcttcg ccttcacgct cggctcccaa cgatcgatca aggagatcgg
     1141 actcggactc ggttccgcga tcctgatcga cgtgctgatc gtccgcatga tcgtggtccc
     1201 gtccttcatg tcgctggcgg gaaatgccaa ctggtggttc ccggcgtggc tcgaccggat
     1261 cctgccgaac ctgaacgtcg agggcgacac tgcgtcgacg gatcacagcg atccgaccga
     1321 tggcacgaca ccgaagccgg tgattgccac ccagtcctag tcgttccatc actcgatgcg
     1381 cggttgacaa cggtgtgaac cgatgcggaa gcggcccggg gattcttccc cgggccgctt
     1441 cctgtttccg gcatccgtct tcgtcgccgc gcgagccgac cgggtggcaa gctgccgttt
     1501 gaggaccggg tcacgtcaat atgagacgta tggcgcggcg tcacaggacc aggcgctggc
     1561 agcatccgct ggcgatgcat ataaccgcat gcgaaaataa gcgttaatca aaggataatc
     1621 atttctgttc tcgcgctcat aagagaggtt gctgacgatg gttgctgcta ccccttccgg
     1681 tggaattcac gttgttgctg ccgtcgatat tggctcgaac acgatcaaga tgagcatcgc
     1741 gcggcaggct gaagcaaatg cgaacggatt cgaggaactc gcctcgggca gtcacactgt
     1801 ccgtctcggc gcaggtctcg ccgaaaccgg gcgcctcgcg gacgatcgca tcgcctccgc
     1861 cctcgaaacg ctcaccgcgt tcgctggcga ggcgcgtcgg catggtgcga cgcgcctcgt
     1921 cggtgtggcg acggcggcga cgaggcaagc ggcgaatgga ccggagttcc tggcgagcgt
     1981 cgcgaacgag acgggctggg atgtccgcgt cgtcagcggt gacgaggagg ccgcgctcac
     2041 cttccgtggg ctggcgagcg agcacgatct ttccgggatc gtggtcgttg ccgacatcgg
     2101 tggcggcagc accgaagtga tcgttgccga gcacgaggtg atccggtcga gtcaatcgct
     2161 gcccctggga tcgggggcgc tgaccgagcg tttcgtcgtc tcggacccgc cggctaccgc
     2221 cgaactggac gcgtgtgcta cggcggcgat cgatgcgctg accgtcatcg attggcccgc
     2281 tgccggcggt cgtgcccacc tgatcgttgt cggcgggacg gccgaggcgg ttgcgaagct
     2341 gctcccgccg aaagcgccgg tgacccggac ggcgatcgag caggcgattg cgacctgccg
     2401 ggacttgtcg tcaacggaat tgagcgaccg cttcgaactc ccgccggcgc gggcacgggt
     2461 cctgccggca ggattgacga tcatcgcaga actcgcgcgg atggtcgcgc cggatcgcat
     2521 ttccctggca cggagcgcca tccggtccgg cttgttgctt gcgacgttcg acgatctccg
     2581 cgcccagacc tctgcgccga ccagtccctc ctcttccggg ggtgattgac gcgcatttac
     2641 cgaagggact ctcatgacga aaacgccggc tcccgagcgg gagaatcacg acgcgacata
     2701 caaggcggcg atgcgacagg cgattgcggc gcgctgggcc gatacgtggc gcgagttggc
     2761 gaaggtcgcc aaatccgaag accccgaggc tgtccacgcg acccgcgtcg cctcgcgccg
     2821 gttgcgggcg gcgatggatg ccgccacgga tgcattcccc cgcaagtggt atcgaaagct
     2881 gcacaagatg gcgaagacga tcacgagcga actcggcacc gtccgtgaca cggacatcct
     2941 tcttcaggaa ctgcaggcgg agcgcgccag ggcggatgcc tccgaatggg tgggact
//
LOCUS       DASTMD010000104         4059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2063231, whole genome shotgun sequence.
ACCESSION   DASTMD010000104 DASTMD010000000
VERSION     DASTMD010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2063231"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /gene="rpsF"
                     /locus_tag="VFQ54_01650"
     CDS             complement(<1..440)
                     /gene="rpsF"
                     /locus_tag="VFQ54_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU0163715.1"
                     /translation="MVGYAREPRAYELMVMIVPDISDEEISEELGRINGYISDIGATI
                     KEVLTDSPWGRRRLAYSIRHNSVDYRDGYYVIYHFDTRPNSIGDMERELKLDVRVIRY
                     LLVHDDPKWGSQNQDSQEGGEGGAEDGASSSPATPVAAVAAPAAP"
     gene            complement(618..1817)
                     /gene="cysS"
                     /locus_tag="VFQ54_01655"
     CDS             complement(618..1817)
                     /gene="cysS"
                     /locus_tag="VFQ54_01655"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU0163716.1"
                     /translation="MRLYNTQSESTEEVVPRDGHIGIYVCGVTPYDTTHAGHAFTYIV
                     YDVLIRYLRAQGVRVTYVQNVTDIDDDVLRKAKELGVDWKDLGRRETDRFLQDMRDLH
                     ALPPDHYVAATDHIPEVIAITSKLVDLGLAYESSGSVYYSVEKDPDFGKLSHLAKDEM
                     LPVANEHGNFPDDPKKRNPIDFVLWQAMAPGEPFWESPWGPGRPGWHIECSAMSTKYL
                     GSQVDIHGGGSDLIFPHHECEIAQTEPVTGTEPFVRYWMHAAMVAYQGEKMSKSLGNL
                     VKVSDVFQTYSSDALRLYLLSNHYRTPWEFEDDRLDDWASVADALTEAAEVQVSGIGD
                     ALDTRVLRDRFFTALDNDLDTPTAIAVLQEFGQAILEAPEDDDIRDAQSELRELGEIL
                     GLTLRDA"
     gene            2130..2816
                     /locus_tag="VFQ54_01660"
     CDS             2130..2816
                     /locus_tag="VFQ54_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163717.1"
                     /translation="MKEIRSARDEISRYGRRIVTILAIVAIAILPQLTAAQSDGNDPD
                     ATPPVNPSAGDATKPCFNGLLRVRDLEQADATLVAGMADAEKKAKASFQPDVRLYSLR
                     LGCPLLASGLQWNGTYFSETAQAYYSTDTGEVQAAEDLPNTIPTLDITKLSFRDVYRS
                     LLRAGFGGDLMITASGGVTVKESTDTQPFGPPNAPKNVVYVHLAIEDRNEVKDIWVDA
                     TNGTVYRYEL"
     gene            2851..3678
                     /gene="otsB"
                     /locus_tag="VFQ54_01665"
     CDS             2851..3678
                     /gene="otsB"
                     /locus_tag="VFQ54_01665"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF014418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEU0163718.1"
                     /translation="MKAASVADVLDKVIDVLRHEPAGVLADFDGTLSPIAPVPEAAIL
                     HPALVDVLPLLKDQLNLLAVITGRAASDAQAKVGVVDMLYVGNHGLEWLEGGDYEAHP
                     AGVEAQQGIVSALTEIEADLRNAGVDVGGFVFENKRLSASIHYRVAADPERVEAILPG
                     ITAARAAEHGLQMTTGKMMVELRPTSTISKGSALVEIASRRGLKGVVFLGDDITDVDA
                     FRSLRRMREDGTIASGLAIGVLSEEVKQPIVDESDILLEGVDAVAELLVALSAHLTR"
     gene            3756..>4059
                     /locus_tag="VFQ54_01670"
     CDS             3756..>4059
                     /locus_tag="VFQ54_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEU0163719.1"
                     /translation="MTATLRHIVAIFLIAIAAIGLTAPSIAMAQAVAPDAPPWLQPAP
                     TPPAQATITVGSTRLDVQIADTPFLQELGLGYRNGLADGTGMLFINGQPASQSYWMK"
BASE COUNT          748 a         1269 c         1278 g          764 t
ORIGIN      
        1 ggagcggcgg gggccgccac cgcagcgacc ggagtcgccg ggctgctgga agcgccatct
       61 tctgcgccgc cctcgccacc ttcctggctg tcctggttct gcgaacccca cttcggatcg
      121 tcatggacga gcaggtagcg aatcacgcga acatcgagct tgagttcgcg ctccatgtcg
      181 ccgatcgagt tgggccgggt gtcgaagtgg tagatgacgt agtagccatc acggtaatcg
      241 acgctgttgt ggcgaatcga ataagcgagg cggcggcgtc cccaggggga gtccgtcagc
      301 acttccttga tcgtggcgcc gatgtcgctg atgtaaccat tgatccgacc aagctcctcc
      361 gagatctcct cgtccgagat atccgggacg atcatgacca tcaattcgta ggcgcgtggt
      421 tcacgagcat aaccaaccaa ttgaagaacc ccttccttcg ggtttccccc ctgtggctta
      481 ccgcaagcgg cgggattaca cgggaggaga aaggacgcgg atcgatccgc gttctagacg
      541 acacaccaag aaggccggat gaactccggc ccacggcggg caagtatacc atcaacgcac
      601 gttgtccgac gggcggctta cgcgtcccga agagtcaggc cgaggatctc cccgagttcg
      661 cgaagttccg actgcgcatc ccggatatcg tcgtcttcgg gggcctcgag aatagcctgg
      721 ccgaactcct ggaggacggc gatcgccgtc ggcgtatcga gatcgttgtc tagcgcggtg
      781 aagaagcgat cgcggagaac tcgcgtatcg agtgcatcgc cgatcccgga tacctggact
      841 tcggcggcct ccgtcagcgc gtcggcgacc gaagcccagt cgtcgaggcg gtcgtcctcg
      901 aactcccatg gcgtgcggta gtgattcgag aggaggtaga gacgaagcgc gtccgacgaa
      961 taggtctgga agacatcgga gaccttgacg agattgccaa gggacttcga catcttctcg
     1021 ccctggtagg cgaccatcgc ggcgtgcatc cagtagcgga cgaacggctc ggtccccgtt
     1081 acgggttcgg tctgcgcgat ctcgcattcg tgatgcggga agatcagatc cgagccaccc
     1141 ccgtgaatgt cgacctgcga gccgaggtac ttcgtcgaca tcgcgctgca ctcgatgtgc
     1201 cagccgggac ggcccggtcc ccacggcgac tcccagaagg gttcgccggg cgccatcgcc
     1261 tgccagagca cgaagtcgat cggattgcgc ttcttcggat cgtccggaaa gttcccgtgc
     1321 tcgttggcga ccggcagcat ctcgtccttc gcgaggtggc tcagcttgcc gaagtcggga
     1381 tccttctcga cggaatagta gacactgccg ctggattcat aggcgagacc gaggtcgacc
     1441 agtttcgacg tgatcgcgat cacctcgggg atgtggtccg tcgccgcgac ataatgatcg
     1501 ggcggcaggg cgtgaagatc gcgcatgtcc tggaggaagc gatccgtctc gcggcgcccg
     1561 aggtccttcc agtcgacgcc gagttccttc gccttgcgga ggacatcgtc atcgatgtcc
     1621 gtgacgttct ggacataggt cacgcgaacg ccctgcgccc ggaggtagcg gatgaggacg
     1681 tcgtacacga tgtaggtaaa cgcgtgcccg gcgtgcgtcg tgtcgtacgg tgtgacaccg
     1741 cagacgtaga tgccgatgtg gccatcgcgc gggacgactt cttccgtgga ttcggactgg
     1801 gtgttgtaca gccgcatgca ggcatgctcc gcagggtgat gtggatcgcg caacacgacg
     1861 cgcgatctcg aatcgtcaag tctctcgtga tcgcgaccgg gatctggtta tttcaccggg
     1921 agttgtcctg gagatgcgct cgtcatcgag cccgaatcca ggctccgagc ttgtcgcgag
     1981 taacgtcctg agtatatctc agtaactcac cccgcacgcg cggttcgttc ggttcgtccg
     2041 gatgccggga ggacgatcgc gaccgcgtga tatgatcgaa tccggatcgg ttgtcgtgag
     2101 tgtatgtgca gggaggtcaa ggcttcgtga tgaaggagat tcggtcggcg cgagatgaga
     2161 tcagccgcta cggccgcaga atcgtcacga tcctggcgat cgtcgcgatc gcgatccttc
     2221 cccagctcac cgccgcccag tccgacggaa atgaccccga cgcgacaccc cctgtcaatc
     2281 cgtcggccgg cgatgccacg aagccctgct tcaacggcct gcttcgcgtt cgggatctcg
     2341 agcaggccga tgccacgctt gttgccggaa tggcggacgc ggagaagaag gcgaaggcat
     2401 ccttccagcc tgacgtccgg ctctacagcc tccgcctcgg atgcccgctt ctcgcatccg
     2461 gcctccagtg gaacggaacc tacttctcgg agaccgcgca ggcgtactac agcaccgata
     2521 ccggtgaggt ccaggccgcc gaagaccttc cgaataccat ccctaccctc gatatcacga
     2581 agctctcgtt tcgggacgtc tatcgatcgc tgctccgcgc cggctttggc ggcgacctga
     2641 tgatcaccgc gtcgggcggt gtcacggtca aggaaagtac ggatacccaa ccgttcggcc
     2701 ctcccaacgc accgaagaac gtcgtctacg tccatctcgc gatcgaggac cgcaacgagg
     2761 tcaaggatat ctgggtggat gcgacgaacg gaaccgtcta ccgctacgag ttgtagcact
     2821 cctcgcaatc acgacccaaa ggaacttcga atgaaagctg caagcgttgc cgacgtgctc
     2881 gacaaggtca tcgatgtgct ccggcacgaa ccagcgggtg tgctcgcgga cttcgacggc
     2941 acgcttagcc cgatcgcgcc ggtgcctgag gccgcgatct tgcatcccgc gctcgtcgat
     3001 gtattgcccc tgctcaagga ccagttgaat ctcctggcgg tgatcaccgg acgcgccgcg
     3061 agtgatgccc aggcgaaggt tggcgtcgtc gacatgctct atgtcggcaa tcatggcctg
     3121 gagtggctcg aaggtgggga ttacgaggct catcccgccg gcgtcgaggc tcagcagggc
     3181 atcgtctcgg cactcaccga aatcgaggct gacctgcgga acgcgggcgt cgacgtcggc
     3241 ggcttcgtgt tcgagaacaa gcggctttcc gcgtcgattc actatcgcgt tgccgccgat
     3301 cccgaacgcg tcgaggcgat cctgccggga atcaccgcgg cgcgggcggc ggagcacggc
     3361 ctgcagatga cgaccggcaa gatgatggtc gagcttcggc ccacctcgac gatctcgaag
     3421 ggatccgccc ttgtcgagat cgcgagccgg cgcggactca agggtgtcgt ctttctcggc
     3481 gatgacatca cggacgtcga tgcgtttcgc tccctgaggc ggatgcgcga ggatggcacg
     3541 attgcctcgg gtctcgcgat cggtgtcctc tcggaggaag tgaagcagcc gatcgtcgac
     3601 gagagcgaca tcctccttga aggcgtcgac gcggtggcag aattgctggt ggcgctctcg
     3661 gcacacctga cgcgctgatt gcacagccac gagccttcct gcatcatggt atcgaagctc
     3721 gcccctgatg tacccgggaa cacgggagac caccgatgac cgccaccctc cgccatatcg
     3781 tcgccatctt cctgatcgcg atcgccgcga ttggtctcac cgcgccgtcg atcgccatgg
     3841 ctcaggccgt cgctcccgac gctccaccct ggttgcagcc tgcgcccacg ccgccggcgc
     3901 aggcgacgat cacggttggc tcgaccaggc tggacgtgca gatcgcggat acgccgttcc
     3961 ttcaggagct tggactcggc tatcgtaatg gactcgccga tggtaccggg atgctgttca
     4021 tcaacggcca acccgcctcg cagtcctact ggatgaagg
//
LOCUS       DASTMD010000105         1590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2598834, whole genome shotgun sequence.
ACCESSION   DASTMD010000105 DASTMD010000000
VERSION     DASTMD010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2598834"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(331..1269)
                     /locus_tag="VFQ54_01675"
     CDS             complement(331..1269)
                     /locus_tag="VFQ54_01675"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEU0163720.1"
                     /translation="MATHHLRPSRETLHGTFSHDFPPVLTIEPDDTVVFETLDAGWTI
                     EAPKDQLTEGTVFSPKDPEKDRGHALVGPVFVSGAKPGMVLVVEIGAIVPGSYGWNFG
                     SVNVNGVNDRLGLTGDRIRLGWNLDSEKGIATDQYGHSVKMRPFFGVMGIAPAEDGYL
                     LTSPPRVTGGNIDCKELVAGTTLFLPIAVEGALFSCGDGHARQGDGEVSTTAIECPIA
                     SGTLTFSLRDDLSLTTPMAWTPEGWLTFGFHEDLDEAALIALNAMLDLLERDHGIERK
                     VGLALASVVVDLRVTQIVNGVRGVHAVLPHGAIEGL"
     gene            complement(1335..>1590)
                     /locus_tag="VFQ54_01680"
     CDS             complement(1335..>1590)
                     /locus_tag="VFQ54_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163721.1"
                     /translation="PLTVYVISVIFIVMAPLFQGIGIVDVGIVIALERLGVPATMAIS
                     STLISRILGLWLPLAIGILTQFVEAWWSRRRTARSHSPAI"
BASE COUNT          288 a          546 c          494 g          262 t
ORIGIN      
        1 ctgcgcagtc ccgccccacg tgccaccgct gcgtcaatgg tgattcccgg gcatccgtcc
       61 cggcacccca cgtgggtgcc tccccgacgt cagggcaacg cgccacggaa acgaaacgtg
      121 agacggcgag gggggacatt gaagtccccc cgtactcacg accgccgcat ccacgatcgc
      181 gaccggtggt catcacccat ggcacgtcgt tggaacggcc ggggtttgtg gagttcgccc
      241 tgagcttgtc gaagaggcgg cagttatctc tggccgtgaa cgaggacgtc ccatccctgt
      301 cccatccctg gggtcctgca tccctgccgt ctacaggccc tcgatggccc cgtgcggcag
      361 gaccgcgtga acaccgcgca caccgttgac gatctgcgtc acacgcaggt cgacaacgac
      421 gctcgcgagc gccagcccga ccttgcgctc gatcccgtga tcgcgctcca ggaggtcgag
      481 catcgcattc agggcgatca gggctgcctc gtcgagatcc tcatggaaac cgaaggtcag
      541 ccagccttcc ggcgtccacg ccatcggtgt cgtcagcgaa agatcgtcgc gcagcgagaa
      601 ggtgagggtg cccgaggcga tcgggcactc gatcgccgtc gtcgagacct cgccatcgcc
      661 ctgccgcgca tgaccgtcgc cacaggagaa gagcgcaccc tcgaccgcga tcggcaggaa
      721 gagcgtcgtc ccggcgacga gttccttgca gtcgatgttg ccgccggtca cgcgcggcgg
      781 tgaggtcagc aggtagccgt cctcggccgg ggcgatgccc atcacgccga agaagggccg
      841 catcttcacc gagtgaccgt actgatccgt cgcgatgccc ttctcgctgt cgagattcca
      901 gcccagacga atccggtccc cagtcagtcc gaggcgatcg tttactccgt tgacgttcac
      961 gctgccgaag ttccagccgt agcttcccgg cacgatcgcg ccgatctcga cgacgaggac
     1021 catgcccggt ttcgccccgc tgacgaagac cggcccgacg agcgcatgcc cgcgatcctt
     1081 ctccggatcc ttcggcgaga agacagtacc ctccgtcaat tgatccttcg gcgcctcgat
     1141 cgtccacccc gcgtcgagcg tctcgaagac gacggtgtcg tccggctcga tcgtcaggac
     1201 cggtgggaaa tcgtgggaaa aggtgccgtg cagcgtctcg cggctcgggc gcaggtgatg
     1261 cgtcgccatc gggatggttc tcctcgccgg ccattcgtgc cggacagctt cttcggttga
     1321 aacgggaagg acgactagat cgccggggaa tggctccggg cggtgcggcg gcgcgaccac
     1381 caggcttcca cgaattgcgt caggataccg attgccaggg gaagccacaa tcccaggatg
     1441 cgcgaaatca gcgtcgaact gatcgccatc gtcgccggca cgccaagacg ctcgagcgcg
     1501 atcacgattc cgacatcgac gatgccgatc ccctggaaca acggcgccat cacgatgaag
     1561 atcacgctga tcacgtagac cgtcagcggc
//
LOCUS       DASTMD010000106         3557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2599223, whole genome shotgun sequence.
ACCESSION   DASTMD010000106 DASTMD010000000
VERSION     DASTMD010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2599223"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFQ54_01685"
     CDS             complement(<1..664)
                     /locus_tag="VFQ54_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0163722.1"
                     /translation="MSHTVPIYLAGEWVETADELDVVNPYDGSVVGTTYLAGQPQLET
                     AIQAAVDVFPTFGRLPVFERVELLKALAAKLRENRQLVIETIVREAGKVIGEATTEVD
                     RGVFTIETAAEEAKRIEGDVIPLDLLPSSKGRFGVVRRFPIGPVAGISPFNFPLNLAL
                     HKVAPAIAAGNPIVLKPPSLAPLTMLLIARLVDEIGLPKGALSVLPMNREVGNAMVGD
                     PRF"
     gene            complement(779..1288)
                     /locus_tag="VFQ54_01690"
     CDS             complement(779..1288)
                     /locus_tag="VFQ54_01690"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEU0163723.1"
                     /translation="MFDEVKGFGVTLKRMVSPKTTTEYPEEKRPMEPRFRGLPSLRSD
                     PDTGEALCVACGLCARICPTSCLEMHVIPSEEGDRELGEFILKAGRCMFCGLCSQVCP
                     VDAITMSQEYELAALEREGLIYTKTELSTIGAQAPIWWDSGVGEENQQTNAPTRYDRF
                     REQASKLYE"
     gene            complement(1350..>3557)
                     /locus_tag="VFQ54_01695"
     CDS             complement(1350..>3557)
                     /locus_tag="VFQ54_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0163724.1"
                     /translation="CATPLGEGMEVMTHSPDALQARQFVLQMYLIDHALDCPTCDKSG
                     ECYLQDNTYLHNINANPYRRPKLSQPYEHFSPTIDYKWDRCIMCNRCTRVCDEVIGVT
                     AIESANRSLEATITPAFGMDLSDGTCTNCGMCIAVCPVGALTDRHFGHHPWELDTTET
                     ICGFCDGGCTINVESNKGIVRRTTHLWERGVNHGYTCEKGQWGHEQIQSPSRLFYPRI
                     REENGDVFESTWEEAIARVADQFGHYQGSKFATLASGENTNEEAYALQQFSRAVMQSN
                     NVNRFLSPAQSAVERVTRQAFGVDVSSTNNLQELFTAAKNIMVVGPSIFEHAPIVSYW
                     INHSRLYRESRIVVVSTDRYPLCDRAAIWLKPNEGTTNLLLDAIGSVILSTALLSPTA
                     ATMDGYAELAERLGAIDLESVATQTGVPVESITDAAKIFVTGDAEFAGAIPDGGFVPA
                     AVFHTLAHVDEQANANAYGDPALVAAACANLASLTGNVGRAGGGVASMRGPANYQGTT
                     DMGLTPDLLPGGATISDANARGRFEDAWSSRWVSEAITSNGFVQRQTLPTTPGIELDA
                     LVDAIDAGTIEAMWIEGGYATRHEVLDSRLYESLKKLKYLVVVDAYDSPLTAIADVVL
                     PLALNLEKDGTFTSYDRTVQRVRAAVPPMGESRSELQILGAVAAQLGYSLAWDHPAQA
                     MTEISRLVPDYAGVTYARLERGGIVTPVATFGEQGETVLTTERLSSRFVNLFA"
BASE COUNT          623 a         1085 c         1207 g          642 t
ORIGIN      
        1 tgaagcgcgg atcgccgacc atcgcgttgc cgacctcgcg attcatcggc agcacgctca
       61 gcgctccctt gggaagcccg atctcgtcga cgagcctcgc gatgagcaac atcgtcagcg
      121 gggcaaggga gggcggcttg agcacgatcg gattgcccgc ggcgatagca ggagcgacct
      181 tgtgcagggc gagattcaag gggaagttga atggcgagat gccggcgacc ggcccgatcg
      241 ggaatcggcg gaccacgccg aagcgccctt tcgatgacgg caggagatcg agggggatga
      301 cgtcgccttc gatccgcttc gcctcttcgg ccgccgtctc gatcgtgaag acgccacgat
      361 cgacctccgt cgtggcctcg ccgatcacct tgccggcttc acgaacgatg gtctcgatca
      421 cgagttgccg gttctcgcgg agcttcgccg cgagtgcctt caggagttcg acccgctcga
      481 agacggggag tcgcccgaag gtcgggaaga cgtcgacggc cgcctggatc gcggtctcga
      541 gttggggctg cccggcgagg taggtcgtgc caacgacgct gccgtcgtac ggattgacga
      601 cgtcgagctc gtccgccgtc tcgacccatt cgcccgcgag atagattgga accgtgtgtg
      661 acataaaatc tccaggaatg cgctagtcaa gtatcgagta caggctcacg gggaaagcat
      721 acgcccggac cgcaagagca gcccgggcgt ttcgcgattc gtgagtgagg aatgaccgtt
      781 attcgtagag cttggatgcc tgctcgcgga accgatcgta gcgcgtgggg gcgttggtct
      841 gctggttctc ttcgccaacg ccggagtccc accagatcgg agcctgggcg ccgatcgtcg
      901 aaagttcggt cttcgtgtag atgagaccct cgcgctcgag cgcggcgagt tcgtattcct
      961 ggctcatcgt gatcgcatcg accgggcaga cctgggagca gagcccacag aacatgcagc
     1021 gtccggcctt gaggatgaac tcgccaagct cacggtcgcc ttcctcggag gggatgacgt
     1081 gcatctccag gcacgaggtc ggacagatcc gggcgcagag cccgcaggcg acgcagagtg
     1141 cctcgccggt atccggatcg gaccggagcg acgggagacc acgaaagcgc ggctccatcg
     1201 gtcgcttctc ttccggatac tcggtcgtgg tcttgggtga gaccatccgc ttcagcgtga
     1261 cgccgaaacc ctttacttca tcgaacatcg tggcgacctt tccgccgact cctcgcgccg
     1321 gcccgagcaa agatgcagga gtgtgcgtgc taggcgaaga gattgacgaa ccggctcgag
     1381 agtcgttccg tcgtcaggac ggtctcaccc tgctcgccga acgtcgcgac cggggtgacg
     1441 atgccgccac gctcgagccg ggcgtaggtg acgcccgcgt aatccggcac cagtcgggag
     1501 atctcggtca tcgcctgtgc cggatgatcc catgccagac tgtaaccgag ttgcgccgcg
     1561 accgcgccga gaatctgaag ctcgctccgc gactctccca tcggcggcac cgccgcgcga
     1621 actcgctgga ccgtccggtc gtagctcgtg aaggtgccgt ccttctcgag attgagcgcc
     1681 agtggcaaga cgacatcggc gatcgccgtc agcggcgaat cgtacgcatc gacgacgacg
     1741 agatacttga gcttcttcag ggattcgtac agacggctgt ccaggacctc atgacgcgtc
     1801 gcgtagccgc cctcgatcca catcgcctcg atcgtgccgg cgtcgatcgc gtcgacgagg
     1861 gcatcaagct cgatcccggg cgtcgtcggc agtgtctggc gctggacgaa accgttcgag
     1921 gtgatcgcct ccgagaccca acgcgacgac cacgcatcct cgaagcgacc gcgcgcattc
     1981 gcgtcggaga tggtggcgcc tccgggcagc agatccgggg tcagacccat atcggtcgtg
     2041 ccctggtagt tggcgggtcc ccgcatcgag gcgacaccgc cgccggcccg accgacgttg
     2101 ccggtcagcg aggcgaggtt cgcacaggcc gcggcgacga gcgcggggtc accataggca
     2161 ttcgcgttcg cctgttcgtc gacatgcgcc agcgtgtgga agacagcggc tggcacgaaa
     2221 cctccgtccg gaatcgctcc tgcgaattcg gcgtcgccgg tgacgaagat cttcgccgcg
     2281 tcagtgatcg attcgaccgg gacaccggtc tgcgtcgcaa ccgattcgag gtcgatcgcg
     2341 ccgagccgct ccgcgagttc cgcgtatccg tccatcgtcg cggcggtggg tgagaggagt
     2401 gccgtggaaa gaatgacgga gccgatcgcg tcgagcagga gattggtggt gccttcgttc
     2461 ggcttgagcc agatcgccgc ccggtcgcag agcggatagc gatccgtgct gacgacgacg
     2521 atccgcgact cccggtagag gcgggagtga ttgatccagt aggagacgat cggcgcatgc
     2581 tcgaagatcg acggcccgac gaccatgatg ttcttcgcgg cggtgaagag ttcctggaga
     2641 ttgttcgtgg aggagacgtc gacgccgaac gcctgccgcg tcacacgctc gacggcggac
     2701 tgggccggcg agaggaagcg gttgacgttg ttcgactgca tgaccgcgcg ggagaactgc
     2761 tggagggcat acgcctcctc gttcgtgttc tcaccggaag ccagggtggc gaacttcgat
     2821 ccctggtagt ggccgaactg atcggcgaca cgcgcgatcg cttcctccca ggtcgactcg
     2881 aagacatcgc cgttctcttc ccggatccgc gggtagaaga ggcgggaggg tgactggatc
     2941 tgctcgtgtc cccactggcc cttctcgcag gtgtagccgt ggttgacgcc gcgctcccag
     3001 aggtgcgtgg tccggcgaac gatgcccttg ttggattcca cgttgatcgt gcagccaccg
     3061 tcgcagaagc cgcagatcgt ctcggtcgta tccagctccc agggatggtg gccgaaatga
     3121 cgatcggtga gggcgccgac cgggcagacc gcgatgcaca tcccgcagtt ggtgcaggtg
     3181 ccgtcactga gatccattcc gaaggctggg gtgattgtcg cctcgaggct gcggttcgcc
     3241 gattcgatgg cagtgacgcc gatgacctca tcgcagacgc gcgtgcagcg gttgcacatg
     3301 atgcagcggt cccacttgta gtcgatcgtc gggctgaagt gctcgtatgg ctgggagagc
     3361 ttggggcgac gataggggtt cgcgttgatg ttgtggaggt aggtgttgtc ctgcaggtag
     3421 cactcgccgg acttgtcgca ggtcgggcaa tcgagcgcgt gatcgatgag gtacatctgg
     3481 aggacgaact ggcgcgcctg aagggcgtcg ggcgagtggg tcatcacttc catgccctcg
     3541 ccgagcggcg tcgcgca
//
LOCUS       DASTMD010000107         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_813745, whole genome shotgun sequence.
ACCESSION   DASTMD010000107 DASTMD010000000
VERSION     DASTMD010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813745"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VFQ54_01700"
     CDS             <1..1307
                     /locus_tag="VFQ54_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEU0163725.1"
                     /translation="PTQQNGLFEKDVDGDGTLVTMRIKDPAGPWTPSSHDPRIMRRRG
                     PDEFGGDYYFVLPEGEIQNWDGASVPLAPDFYGIDANRNFPADWAPAWVQYGAGAYPL
                     SEPETKALAEFMMAHPNIHGSQHFHTYSGCILRPPTSYPTADMPALDRAVFTAIGKMG
                     EEETGYPCIGIYDDFAYDRKKAMKGGLLDWVYEQLGVIPFSTELWSLTAKAGIEVTDF
                     IEFFRNRSDEVDAAMLRVLDEEVGGEGFREWTPFAHPQLGPVEIGGWRRTFTWVNPPG
                     PMLEEVTSTNAKFVLRAAQTAPRLEIRDATAELLADGLYKVSLVAHNLGFLPTYVTDV
                     ARSSGVSKPVVATISLAEGAELVTGDLETELGHLQGRANTLGEMSFNDTYPIQNRARA
                     EWVVRQPAGSSVTVTVACARAGTSRAEIILSQDPALSEPRSL"
     gene            complement(1329..2306)
                     /locus_tag="VFQ54_01705"
     CDS             complement(1329..2306)
                     /locus_tag="VFQ54_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU0163726.1"
                     /translation="MKAIVYQTYGSPDVLELREIEKPPVKDGEVLIRVHAASANPADW
                     HLMRGSPFLARLGTGLLKPKRTTLGVDVAGTVEAVGRGVTQFRPGDEVFGERFQSGGS
                     GFAEYVAVAEDRIALKPANLSFEAAAAAPLAAMTALQGLRDKGRIQSRQKVLINGASG
                     GVGTFAVQIANWFGAEVTGVCSARNLDMVRAIGAQHVIDYTQEDFTRSGKTYDLVYCT
                     VGNRSAADYRRALTANGISVVSGFTTMSHMLLQMLLLGTWVSVTGSRKVGMMGTVSPN
                     REDLVFIKDLLEDGKVVPVIDRRYSLSETADAIRYLEEGHARGKVVITV"
BASE COUNT          575 a          975 c          838 g          535 t
ORIGIN      
        1 atcccacaca gcagaacgga ctcttcgaga aggacgtcga cggcgatggc accctggtaa
       61 cgatgcggat caaggatccc gccggaccgt ggacgccctc gtcgcacgat ccacgcatca
      121 tgcgacggcg cggaccggac gagttcgggg gcgactatta cttcgtgctg cccgaaggcg
      181 agatccagaa ctgggacggc gcctcggtcc cgcttgcgcc cgacttctac gggatcgacg
      241 ccaaccgcaa cttcccggcc gactgggcgc cagcctgggt gcagtacggc gccggcgcct
      301 atcccctctc cgaaccggag acgaaggcgc tcgccgagtt catgatggcg catccgaaca
      361 tccacggctc ccagcacttc cacacctatt ccggctgcat cctgcggccg cccacgtcct
      421 atccgaccgc ggacatgcca gcgctcgacc gcgcggtgtt cacggcaatc ggcaagatgg
      481 gcgaggagga gaccggctat ccctgcatcg gcatctacga cgacttcgcc tacgatcgga
      541 agaaggcgat gaagggtggc ctgctcgact gggtctacga gcagctcggt gtgatcccct
      601 tctccaccga actctggtcg ctcacggcaa aggcggggat cgaggtcacg gacttcatcg
      661 agttcttccg caaccggtcg gacgaggtcg atgccgcgat gctccgcgtc ctcgacgagg
      721 aagtcggcgg cgaaggattc cgcgagtgga cgccgttcgc gcacccgcag ctcggaccag
      781 tcgagatcgg cggctggcgg cgcaccttca cgtgggtgaa tccgccggga ccgatgctcg
      841 aggaggtgac gtcgaccaat gcgaaattcg tgctgcgcgc ggcgcagacc gctccccggc
      901 tcgagattcg cgacgccacg gcggaactcc tcgccgacgg tctctacaag gtctcactcg
      961 ttgcccacaa tctcggcttc ctgccgacct acgtcaccga cgttgcccgc agcagcggcg
     1021 tgagcaaacc cgtggtggcg acgatctccc tcgccgaggg agcggaactg gtgaccggcg
     1081 acctggagac cgaactcgga cacctccagg gccgcgcgaa cacgctcggc gagatgtcgt
     1141 tcaacgacac ctatccaatc cagaaccgcg cccgtgcgga atgggtcgtt cgccagcctg
     1201 ccgggtcatc ggtgacggtg accgtcgcct gtgcaagagc cggaacctcc cgcgccgaga
     1261 tcatcctcag ccaggacccg gcgctcagcg agccgcgatc cctgtagaaa tcagggaatc
     1321 ccccggtttc agacagtgat gacgactttt cctcgagcgt gcccttcctc gagataccga
     1381 atggcgtcag ccgtctcact gagcgaatag cgtcgatcga tgaccggaac gaccttgcca
     1441 tcctcgagaa gatccttgat gaagaccaga tcttccctgt ttgggctcac cgtccccatc
     1501 atgccgactt tcctgctccc cgtcactgac acccaggtgc caaggagcag catctgcaac
     1561 aacatgtgcg acatggttgt gaatccggaa acgacagata ttccgttggc ggtcaatgca
     1621 cgcctgtaat ccgcggcgga acggttgccc acggtacagt agaccaggtc atacgtcttt
     1681 ccgctccgtg tgaagtcctc ttgcgtgtag tcgatgacgt gctgtgcacc aatcgcgcgc
     1741 accatatcca ggttccgcgc actacacacc cccgtcacct cagcaccaaa ccagttggcg
     1801 atctgcaccg caaacgtccc tacaccacca gatgccccat tgatcagcac cttttgccgt
     1861 gactgaatcc gtcccttgtc gcggagcccc tgcagtgcgg tcatggccgc cagcggcgcg
     1921 gcagcggccg cttcgaacga gaggttcgcg ggtttcaacg ctatcctgtc ttccgcaacg
     1981 gccacatatt cggcaaaacc actcccgccc gactggaatc tctcgccaaa gacctcatcg
     2041 cccggtcgga actgcgtgac accacggcca accgcttcta ccgtccccgc tacgtcaact
     2101 ccgagcgttg tacgtttcgg cttgaggagc cctgttccaa ggcgcgcaag aaatggcgat
     2161 cctcgcatga gatgccagtc ggccgggttt gcggatgcag catgaacccg tatcaggact
     2221 tcaccgtcct tgacaggggg cttctctatc tcccgcaact caaggacatc gggcgatcca
     2281 taggtctgat agacgatagc cttcatgcga atctgcttcc ttgtgtatcg agaccagctg
     2341 tcgctacgcc gtcaccaggg agcccttgca aggattctcg cgagccaatc gtgctttggc
     2401 atcgctgatc ctgcgatgag aaatccacct cacaaccagg agagacacgt gattcaacca
     2461 attttcggag agaagctctc tacgcatact acacccgggg tccattcccg tcgggatgat
     2521 cctcgtgacg gcggtcgtgg tggttcttct cgcggaatca cgccactacc agtcgtcagg
     2581 gacctcgcac ggccgcgccc cgctccgcgc gaccgcaccg ctgaaacggc aacggttcgt
     2641 agagcgtcac acccatttcc ctgcagttgt gaccggaacc gttccattaa caggacaggc
     2701 gatgtccgcc ttcgcatcca tgatgcaggt atcccgatgc tgcgggatgt gcgttcgtaa
     2761 aggggggaaa ccggatggat ctactcgatc gcatgctgga tcacgatcgc tggacgacgg
     2821 cacgtgtgct ggacctgagt cgcggtctga gcgacgcgga actcgatcga cggttcgaca
     2881 tcggacacga gacgctccgc gcgacgtacg agcacatgat ctt
//
LOCUS       DASTMD010000108         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_902578, whole genome shotgun sequence.
ACCESSION   DASTMD010000108 DASTMD010000000
VERSION     DASTMD010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902578"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFQ54_01710"
     CDS             complement(<1..300)
                     /locus_tag="VFQ54_01710"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0163727.1"
                     /translation="MTKTTVYLVRHGETDWNRIRRYQGTTDIPLNATGEQQAAQLAAY
                     LGTHELAPEGDAIVSSPLMRAFVTARAIGDALGITEILTDDDLMERAYGVAVGSTL"
     gene            583..>2217
                     /locus_tag="VFQ54_01715"
     CDS             583..>2217
                     /locus_tag="VFQ54_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163728.1"
                     /translation="MRNLLGRLNARSLDSLRRIARFWSIALPGGDERGRFVGALYRTM
                     TDIRSIRQAWEQLDATSQRIIRELALSEADALSVEDIAAAVALPVDQTRDALVRLFQA
                     GILAREGDSQELPIGAVPKLFLPREMGLLFRRLQDEIDAGDLSRSSLRVLLEITDDAD
                     VEAAATRWGIRVIPGMRRRADLIDEILRNVSQPERIERLMRELPTDAAAIWSIVRGSG
                     HDGAMPLEDVVRRVWPSGFSDPRDGLRNEIRMREALATLETSLLVMHAYRRDGTRRLF
                     IPQEILHPGRVPTTLPLRPLQPLPPDTVTAPQTRHPDALAWDLMTVLREISGFGAPVW
                     VPGESLSRSWQRRVNGRLWFGGSEIPPTGYLGFLLTLGLGVGVIEPAAETVSPTTDRN
                     AIRPALATRIRSWRTMSFEDQTAHLKTAWLATDQWTEGREREEIDVWGADWRGFRHRL
                     LEALANVPADEWVLIDDLGARLAEQNPALIGTTFTAASARFADDTRDSRIATIARIIA
                     VEVETALSWFGLVELSPVSGRGIAVHVTDAGRAMSQT"
BASE COUNT          357 a          780 c          698 g          382 t
ORIGIN      
        1 gagcgtcgat cccacggcga cgccgtaggc gcgctccatc aggtcgtcat cggtcaggat
       61 ctcggttatg ccaagggcgt caccgatcgc gcgggcggtg acgaacgccc gcatcagcgg
      121 gctgctgacg atcgcgtcac cctccggcgc cagttcatgc gtcccgaggt acgccgccag
      181 ttgcgccgcc tgctgttcac ccgtcgcgtt gagagggata tccgtcgtgc cctggtagcg
      241 ccggatccgg ttccagtccg tctcgccgtg ccgaaccagg taaaccgtcg tcttcgtcat
      301 caattcactc actgtaccgg ccccgcaggc tcccgtcgtc atgaccgaag tattcccact
      361 cgtaccaacg accactcgaa cgatcgacga acaaacgtcc agtacatcct aaagccattt
      421 cccggcgggc gccttaccgt atcgtgaagc tccggggcca ctcggcacgc atgtcacaat
      481 cgtttcgcct tcatttcctt tcaatgtggc acgattgaga cataatcgaa ggaatgacgg
      541 gcggctcgcg gcacaatcag gcgcatggaa aggaggtgaa tgatgcgcaa tcttctcggt
      601 cggctcaatg cccgctcgct ggacagcctc cgccgcatcg cgcgcttctg gtcgatcgcg
      661 ctccccggcg gtgacgagcg tggccggttc gtcggcgcac tctatcgcac gatgacggat
      721 attcgctcca tacgccaggc gtgggaacag ctcgacgcca cgtcccagcg gatcatccgc
      781 gagctcgccc tctcggaggc cgatgcgctc tccgtcgagg atatcgcggc ggcggtcgcg
      841 ctgccggtcg accagacgcg ggatgcgctc gtccgcctct tccaggccgg aatcctcgcg
      901 cgggaaggcg actcgcagga actgccgatc ggcgccgtgc cgaagctctt cctcccgcgg
      961 gaaatgggac tgctctttcg ccgcctccag gacgagatcg atgccggcga tctcagccgc
     1021 agttcgctgc gcgtcctgct cgaaatcacc gacgatgccg atgtcgaggc ggccgctacg
     1081 cgctggggta tccgcgtcat tcccggcatg cgccgtcgcg ccgacctgat cgacgagatc
     1141 ctgcgcaacg tctcgcagcc ggagcggatc gaacgcctga tgcgcgagct gccgacggat
     1201 gcagccgcga tctggagcat cgttcgcggc agcggtcacg acggcgcgat gccgctcgaa
     1261 gatgtcgtgc gccgcgtctg gccgtccggt ttcagcgatc cccgggatgg gcttcgcaac
     1321 gagatccgga tgcgcgaggc actcgccacg ctcgagacat cgctcctcgt gatgcatgcc
     1381 taccggcgcg acggcacgcg gcgcctcttc attccccagg aaattctcca ccctggtcgc
     1441 gttccgacga cgcttccact ccggcccctg cagccactcc ctccagacac ggtgacggcg
     1501 ccgcagaccc gccatcccga cgcgctcgcc tgggacctga tgacggtgct ccgtgaaatc
     1561 tcgggattcg gcgccccggt ctgggtaccc ggcgaatccc tctcccgctc gtggcaacga
     1621 cgcgtcaacg ggcgcctctg gttcggggga agcgagatcc ctcccaccgg ctacctcggt
     1681 ttcctgctca cactcggcct cggggttggc gtgatcgaac cagctgccga aaccgtctca
     1741 ccgacgaccg atcgcaacgc gatccggccg gcgctcgcga cgcgcatccg tagctggcgg
     1801 acgatgagct tcgaggatca gacggcgcac ctgaagaccg cctggctggc gaccgaccag
     1861 tggaccgagg ggcgtgagcg cgaggagatc gacgtctggg gcgcggattg gcgcggcttc
     1921 cggcaccgcc tgctcgaggc gctcgccaat gtccccgccg acgagtgggt gctgatcgat
     1981 gatctcgggg cgcgtctcgc ggaacagaat ccggcgctga tcggtacgac gttcacggcg
     2041 gcatcggcgc gattcgccga cgacacgcgc gattcccgga tcgcgacgat cgcccgcatc
     2101 atcgccgtcg aggtcgagac ggcgctctcc tggttcggcc tcgtcgagct gagtcccgtc
     2161 tccgggcggg gcatcgcggt tcacgtcacc gacgccggac gcgcgatgag ccagacg
//
LOCUS       DASTMD010000109         1837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2866959, whole genome shotgun sequence.
ACCESSION   DASTMD010000109 DASTMD010000000
VERSION     DASTMD010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2866959"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFQ54_01720"
     CDS             <1..827
                     /locus_tag="VFQ54_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEU0163729.1"
                     /translation="NVTGSDQVFDMSTFKLLADGQEVYVDTGNQWVAGMLGYSPAYGN
                     VDQITWAAGESHQFALTFLAPTDAQSLVLEAGDQQIDLTTSISSLSALEKAKTKPDAV
                     VPTTIEAKVVKVVNAETILVDINGRQQEVRYLGVDAPTDTDCYAKDATAANSALVKGQ
                     TVRIERQATDVDARGNWVRDVWAKDGDGDYQLVSNSLIGEGAAKADISEPNTRFDGWL
                     TASQADAQSQGLGLWGSCDNETPDSTVPAAPTPAAPTPAASLNGDTTAIRNDDRIS"
     gene            986..>1837
                     /locus_tag="VFQ54_01725"
     CDS             986..>1837
                     /locus_tag="VFQ54_01725"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU0163730.1"
                     /translation="MREIGIFSGTAHLDLAREICAFLDIPLNHAEISRFSNDCLQVQL
                     QANCRENDIFLVQPLVPPVQEHLMELLLMLDAARGASAARITAVIPHYSYARSDKKDM
                     PRISIGGRLVADLLVTAGADRVLTMALHAPQVHGFFSVPVDHLNALNVLAEHFRKTDL
                     TNAVVVSPDLGNAKNATQFARALGVDVAAGSKRRIADDKVVIDTIVGTVEGKHAIVLD
                     DEIATGGSILQLIESLRERDVHKISVACTHGLFTGKAIERLRDATEIDEIVTTNTVPI
                     PQEKRLPN"
BASE COUNT          375 a          633 c          555 g          274 t
ORIGIN      
        1 agaacgtgac cggcagcgac caggtcttcg atatgagcac cttcaagctg ctcgccgacg
       61 gccaggaagt ctatgtcgac accgggaacc agtgggttgc ggggatgctt ggttattcac
      121 cggcatacgg gaacgtcgac cagatcacct gggcggcagg tgagtcgcat cagttcgcac
      181 tgaccttcct cgctcccacc gacgcccagt cgctcgtgct cgaggccggc gaccagcaga
      241 tcgacctcac gacatcgatc tcgtcgctct cggccctcga gaaggcgaag accaagccgg
      301 acgcagtcgt gccgaccacg atcgaggcga aggtcgtgaa ggtcgtcaat gcggagacga
      361 tcctcgtcga catcaacggt cgccagcagg aagtccgcta cctcggcgtc gacgcaccga
      421 ccgacaccga ctgctacgcg aaggacgcga ccgccgccaa ctccgcgctc gtcaagggcc
      481 agaccgtccg gatcgaacgg caggccacgg atgtcgatgc ccgcgggaac tgggttcgcg
      541 atgtctgggc gaaggatggc gatggagact accaactcgt gtcgaacagc ctgatcggcg
      601 agggagcggc aaaggcggac atctccgagc ccaacacccg cttcgacggc tggctgacgg
      661 cgagccaggc agacgcccag tcgcagggac tcggcctctg gggatcctgc gacaacgaga
      721 ccccggattc gaccgtcccg gcggccccga cgccggcggc gcccacaccg gccgcgagcc
      781 tgaacggcga cacgacggca atccgcaacg acgatcgaat ctcctaaacg acaagcgcga
      841 aggcccggag cagttgctcc gggccttccg tgcgtgtaag cctttacaca aaattgattg
      901 cccgatcccc accggcaatg tacgatcccc tcggcaacgg cgccgtcacc gagtccaaaa
      961 ccagcaacga tattctggag acatcatgcg cgagatcggg atcttcagcg ggacggcgca
     1021 tctggacctg gcacgcgaga tttgcgcgtt cctcgatatt ccgctcaacc acgcggaaat
     1081 cagccgtttc agcaacgact gcctgcaagt acagcttcag gcgaattgcc gcgagaacga
     1141 catcttcctc gtgcaaccac tcgtgccgcc ggtgcaggag cacctcatgg agctcctcct
     1201 gatgctcgac gccgcgcgcg gcgcctcggc ggcccggatc acggccgtga tcccgcacta
     1261 ctcctacgca cgctccgaca agaaggacat gccgcgcatc tcgatcggtg gccggctggt
     1321 cgccgatctc ctcgtcaccg ccggcgccga tcgtgtcctg acgatggcac tccatgcacc
     1381 gcaggtccac ggcttcttca gcgtcccggt cgatcacctc aacgccctga atgtgctcgc
     1441 ggagcatttc cgaaagaccg acctcaccaa cgcggtggtc gtctccccgg acctcggcaa
     1501 cgcgaagaac gcgacccagt tcgcacgcgc cctcggcgtg gacgtcgcgg ccggcagcaa
     1561 gcgccggatc gcggacgaca aggtcgtgat cgacacgatc gtcggcacgg tcgagggcaa
     1621 gcacgcgatc gtcctcgacg acgagatcgc gaccggagga tcgatcctcc agctgatcga
     1681 atcgctgcgc gagcgggacg tccacaagat ctcggtcgcc tgcacgcatg gactcttcac
     1741 cggcaaggcg atcgagcgtc ttcgcgacgc cacggagatc gacgagatcg tgacgacgaa
     1801 cacggtgccg atcccgcagg aaaagcgcct gcccaac
//
LOCUS       DASTMD010000110         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_367677, whole genome shotgun sequence.
ACCESSION   DASTMD010000110 DASTMD010000000
VERSION     DASTMD010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367677"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1069
                     /locus_tag="VFQ54_01730"
     CDS             215..1069
                     /locus_tag="VFQ54_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163731.1"
                     /translation="MVAKSDRILTTHTGSLPRPKDLIPMLYARDRGEAYDEVKIAERT
                     HAAVSELVARQREVGIDVVNDGEAGKVLYSTYVQDRLTGYTGTAPAPQFPSLEAQEFP
                     EFYERLMADFKDSRARIAACEGPVTYRGFSALSDDLANLKDAIKALGKLDEVPAGVFI
                     SAASPGIIATYLPDQYYGDDEKYVNAIAAAMKTEYDAIHYAGFILQLDCPDLAFEYTK
                     VLASGRGLSEFRAMVEYRVAALNEATRDIPADRMRMHICWGNGATPHHYDVPLKDIVD
                     LILEARPA"
     gene            1115..1384
                     /locus_tag="VFQ54_01735"
     CDS             1115..1384
                     /locus_tag="VFQ54_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163732.1"
                     /translation="MWESTKLPDGKYLIPGVIDSTTNFIEHPELVAERILRYASVVGP
                     DRVMAGVDCGLATAAGSTMVDREIAWAKLGSLVEGTRIANAQLSQ"
     gene            1549..1764
                     /locus_tag="VFQ54_01740"
     CDS             1549..1764
                     /locus_tag="VFQ54_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163733.1"
                     /translation="MIDSTTNYIEHPDLVAERLTNLAGVVGADRVVPGADCGLSTVAR
                     SSLVGPDVAWAKLGSLVAGTRLASGQA"
     gene            1788..>2296
                     /locus_tag="VFQ54_01745"
     CDS             1788..>2296
                     /locus_tag="VFQ54_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HEU0163734.1"
                     /translation="MNVLFVGDMVGSESCTWLAGRLPDIKREYDIDLTIVNAENIRIE
                     SGETGVTSGMEASAVQKLLDAGVDVITSGNHAWDGPDAETLLAHPQVIRPLNMPEGWA
                     GKGSITLDVAGETVTVVNLADKDAIPVTTPLYGGWSPAAEHGTVIVDMHGTPPAKYGF
                     AHTNDGKVAA"
BASE COUNT          439 a          708 c          753 g          396 t
ORIGIN      
        1 gcgatgctct cggcgggacg ggagtacctg cggcagcagt ggtggatcgc gaccttcccg
       61 ggcatcgccc tggcgctgac ggcgctcggg ttcaacctgc tcggtgacgg gctccgcgac
      121 atcctcgatc ctcgcatgac gaagtagcga cggcgcgttt tatctgcgtt ccgggcgccc
      181 acgagggcgc catacgaaag gatccgacgt cacaatggtt gcgaagagcg atcgcattct
      241 cacgacccat accggcagcc ttccgcgacc caaggatctc atcccgatgc tctacgcccg
      301 cgatcgcggc gaggcgtacg acgaggtcaa gatcgcggag cggacgcatg ccgccgtctc
      361 tgaactggtc gctcgtcagc gcgaggtcgg tatcgatgtc gtgaatgacg gcgaggcggg
      421 caaggtcctc tattcgacct acgtccagga tcgtctgact ggctacacgg gaaccgctcc
      481 cgcgccccag tttccttcgc tcgaagcgca ggagtttccg gagttctatg aacggctgat
      541 ggccgacttc aaggattcgc gagcacggat cgccgcctgc gagggaccgg tgacctatcg
      601 cggtttcagc gccctgagcg acgatctcgc gaacctgaag gacgccatca aggcgctcgg
      661 caagctggat gaggttcccg cgggcgtctt catcagtgcc gcgtcgccgg gaatcatcgc
      721 gacgtatctg cccgaccagt actacggtga tgacgaaaag tacgtcaatg ccatcgccgc
      781 cgcgatgaag accgaatatg acgcgatcca ttacgccggg ttcatccttc agctcgattg
      841 cccggacctc gcgttcgaat acaccaaggt gctggcgtcg ggccgaggct tgagtgaatt
      901 ccgggcgatg gtcgaatacc gcgtcgcggc actgaacgag gcgacgcgcg acatcccggc
      961 ggaccgcatg cgcatgcaca tctgctgggg caacggtgcg acgccgcacc actatgacgt
     1021 tccgctcaag gacatcgtcg atctgatcct ggaagcgcgg ccggcctgac tctcgttcga
     1081 aggggcgaat ccgcgtcacg gtcacgagtg gaaggtgtgg gaatccacga agttgccgga
     1141 cggcaagtac ctcatcccgg gtgtcatcga ttccacgacg aatttcatcg agcatccgga
     1201 actcgtcgcc gagcgaatcc tgcggtatgc ctctgtcgtt gggccggatc gcgtgatggc
     1261 cggcgtggac tgcggtcttg cgacggcggc gggcagcacg atggtcgatc gcgagatcgc
     1321 ctgggcgaag ctcggcagcc tggtcgaggg tacccgcatc gcgaacgcac agttgagcca
     1381 atagcgaccg gcgctcgcgg cccgccagcg cgccgtgggc atcgacaccg tgaacgacgg
     1441 cgaggcgatc aaggttctgc actcgtccta tgcgcagatc atctcgacgg gtggtggagg
     1501 atctggaatg acctcgaact cccggccgga aagtaagtca ccccgggcgt gattgactcg
     1561 accaccaact acatcgaaca tccggaccta gtggcggagc gattgaccaa cctcgccggg
     1621 gtcgtcggcg ccgacagggt cgtgccgggt gccgactgcg gtctttcgac cgtcgcgcga
     1681 agcagcctcg tcggtccgga cgtggcatgg gccaagctcg gaagtctcgt cgcaggaacg
     1741 cgcctggcga gtggccaggc ttagcttaaa gagagcaggg ggcggagatg aacgttcttt
     1801 tcgtcggaga catggtcgga tctgagtcgt gcacctggct tgcgggtcga ctccctgaca
     1861 tcaagcgtga atacgacatc gatctcacga tcgtcaatgc ggagaatatc cgcatcgaaa
     1921 gtggtgagac tggcgtgaca tccggaatgg aggcgagcgc ggttcagaag ctcctggacg
     1981 cgggcgtcga cgtcattacc tccggcaacc acgcctggga tggacccgat gccgagacgc
     2041 tcctcgcgca tccacaggtc atccgcccgc tgaacatgcc ggagggctgg gcaggcaagg
     2101 gatcgatcac gctggacgtg gccggagaga ccgtcaccgt cgtcaacctc gcggacaagg
     2161 acgcgattcc tgtgacgaca ccgctctacg gcggctggag tccggcggcg gagcacggta
     2221 cggtgatcgt cgacatgcat ggcacaccac cggccaagta cggcttcgcc cacacgaacg
     2281 atgggaaggt cgcggc
//
LOCUS       DASTMD010000111         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2778093, whole genome shotgun sequence.
ACCESSION   DASTMD010000111 DASTMD010000000
VERSION     DASTMD010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2778093"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFQ54_01750"
     CDS             complement(<1..259)
                     /locus_tag="VFQ54_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163735.1"
                     /translation="MKGPIVALPVTEHPDTEQGSSHDRPFEERYVAAMEDAQLSRNLT
                     NFQQGWKASREPLVEEIDFQQLRHEMKAAKTDAIDNLDHYLD"
     gene            complement(453..1022)
                     /locus_tag="VFQ54_01755"
     CDS             complement(453..1022)
                     /locus_tag="VFQ54_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEU0163736.1"
                     /translation="MTDSSFGNLYDRFSQRLGTAGGHTHQMTGTGELASAIDTLHPTT
                     GPEPRVVWIAGETATLAPELLDALRARGLEPRVSTGPAEVRDQPLGLAIARATIAETG
                     SSLMVEPTVEGRSVTLMTDILIVLCESDQLVPSLMEAAPILRDVSRDGASYATFITGP
                     SRTADIERTLAVGVQGPGVFHVIFTDRIA"
     gene            complement(1192..2778)
                     /locus_tag="VFQ54_01760"
     CDS             complement(1192..2778)
                     /locus_tag="VFQ54_01760"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEU0163737.1"
                     /translation="MASSPAPILGHSTRGKVSLHDVAVLLHPQDNLLIAKKALLPRTI
                     VPLEDGTEVKIAQMIPPGHKVAVREIAEGEAVRRYGQIIGFASEPIKPGQHIHTHNVA
                     VHQGELKLDYAFGEDYTPVEFVPEAERRTFMGYRRADGRVGTRNYVALLASVNCSSSA
                     TRRVADYFRDNPIMDQYPNVDGVIGLATKGGCGAHYGSDYLGQLQKTMAGIVDHPNVG
                     AYIILSLGCETNQPDDMIANTSLSGEQPLVLTIQQDGGFQKTVERAIEAVKQLLPEAN
                     KATREPISVSELVLALQCGGSDGWSGITANPALGRAADEIVRQGGTVVLGETSEVYGG
                     EHLLTRRAASPEVAQKLVDKIHWWEDYTSMFGASIDNNPSPGNKLGGLTTIYEKSLGA
                     IAKAGTTPMNDVVGYADRITAKGFVHMDTPGYDPVSATGQVAGGCTIIAFTTGRGSAF
                     GFKPAPSIKLATNSDLYAKQEPDMDINCGRMLDGISLDEMGLEIFEKILSVASGEASK
                     SELADVGEEEFNPWIIGATL"
     gene            complement(2817..>3007)
                     /locus_tag="VFQ54_01765"
     CDS             complement(2817..>3007)
                     /locus_tag="VFQ54_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0163738.1"
                     /translation="AYLHRYHAGEEDETRKQLHARQPIGRMGRPEEIAPLALYLASDE
                     AAYVTGSQMVIDGGWTAR"
BASE COUNT          495 a          934 c         1001 g          577 t
ORIGIN      
        1 gatcgaggta gtggtcgaga ttgtcgatcg cgtcggtctt ggcggccttc atctcgtggc
       61 ggagttgctg gaagtcgatc tcctcgacca gcggttcccg cgacgccttc cagccttgct
      121 ggaagtttgt caggttacgc gagagctgcg catcctccat cgccgcgacg tatcgctcct
      181 cgaagggacg atcgtggctg gagccctgct cggtatctgg atgttcggtc acgggaagcg
      241 ccacgattgg acctttcaag ctcgaatcac cttaccgggc gatctctcta caaactccgg
      301 ttgatcggag tcgtgtctct ctgaacgcgt ttgctgtcgt cgccgcacga caaacctggg
      361 tgagtgattc gtcgatgcag cgggcgttcg tagggcggga cttcaatgtc ccgccgcccg
      421 agacgccgaa ggcgtctcgg ggtcagcgcg ggtcaagcga tccgatccgt gaagatgacg
      481 tggaagacac cgggaccctg gaccccgacg gcgagcgtcc gctcgatgtc ggcggtccgg
      541 ctcggaccgg tgatgaacgt cgcgtacgag gcgccgtccc tcgagacgtc ccgaagaatc
      601 ggcgcggctt ccatcaggct cggcaccagt tgatccgatt cacagagcac gatcaggata
      661 tccgtcatca gcgtcaccga gcgcccctcg accgtcggtt cgaccatcag ggacgagcct
      721 gtctcggcga tcgtcgcccg cgcgatcgcg agaccgagcg gttgatcgcg gacttccgcc
      781 gggccggtcg aaactcgcgg ctcgagaccg cgcgcccgca gggcatcgag gagttccggc
      841 gcgagtgtcg ccgtctcccc ggcgatccag acgacgcgtg gctcgggccc ggtcgttgga
      901 tgaagggtat cgatcgcgct ggcgagttcg ccagttcccg tcatctgatg cgtgtgtccc
      961 ccggcggtgc cgaggcgctg ggagaagcgg tcgtagagat tgccaaagga agaatctgtc
     1021 atctcaatgg tttccagggg cgacatagaa gtcgccccgt actcacgaac gctgtacgga
     1081 aatcggtcgt gagtacggcg ggacttctat gtcccgcctt tcggaactga cccacagacc
     1141 gccaacaccg cgttcccggg cgactatcga attggcgcgt gatcggtgcg attacagcgt
     1201 cgcgccgatg atccatggat tgaactcttc ttcgccgaca tccgcaagct cgctcttgct
     1261 cgcctcgcca gatgcgacgg agaggatctt ctcgaagatc tccagcccca tctcgtcgag
     1321 cgagatgccg tcgagcatcc ggccgcagtt gatatccatg tccggttcct gcttcgcgta
     1381 cagatcgctg ttcgtcgcca gcttaatcga gggagccggc ttgaagccga aggcggagcc
     1441 gcgaccggtg gtgaaggcga tgatggtgca gccgccggcg acctgaccgg tcgcggagac
     1501 gggatcgtag ccgggtgtgt ccatgtggac gaagcccttc gccgtgatcc ggtccgcgta
     1561 gccgacgaca tcgttcatcg gcgtcgtgcc ggccttggcg atcgcaccga gcgacttctc
     1621 gtagatcgtc gtcaggccgc cgagcttgtt gcccggtgac gggttgttat cgattgaggc
     1681 accgaacatc gacgtgtagt cttcccacca gtggatcttg tccaccagct tctgggcgac
     1741 ttccggcgag gcggcgcggc gcgtcaggag atgctcgcca ccgtagacct cactcgtctc
     1801 cccaaggacg acagtaccac cctggcggac gatctcgtcg gcggcgcgcc cgagcgcagg
     1861 gtttgcggtg atgccggacc agccgtccga gccgccgcac tggagcgcga ggacgagttc
     1921 cgagaccgat atcggctccc gggtcgcctt gttcgcttcc ggcaggagct gcttcaccgc
     1981 ctcgatcgcg cgctcgaccg tcttctggaa gccgccgtcc tgctggatcg tgaggacgag
     2041 tggctgctcg ccgctcagcg acgtgttggc gatcatgtcg tccggctggt tcgtctcgca
     2101 gccgagactg aggatgatgt aggcgccgac attcggatga tcgacgatcc ccgccatcgt
     2161 cttctggagc tgaccgaggt agtcggagcc gtagtgggcg ccgcagccac ccttcgtcgc
     2221 gaggccgatc acgccgtcga cgttcggata ctggtccatg atcgggttgt cgcggaagta
     2281 gtccgcgacg cggcgagtcg ccgaagagga gcagttgacg gaggccagaa gcgcgacgta
     2341 gttgcgggta ccgacgcgac catcggcccg tcggtagccc atgaaggtcc ggcgctccgc
     2401 ttccggcacg aactcgaccg gcgtgtagtc ctcaccgaag gcgtagtcga gcttcagttc
     2461 gccctggtgg acggcgacgt tgtgcgtgtg gatgtgctgg cccggcttga tcggctccga
     2521 ggcgaaaccg attatctggc cgtagcgacg caccgcctcg ccctccgcga tctcgcgaac
     2581 ggccaccttg tgacccggcg ggatcatctg cgcgatcttc acttccgtac cgtcttccag
     2641 cggcacgatc gtgcggggga gcagcgcctt cttggcgatc agcaggttgt cctgcggatg
     2701 gaggagcacc gcgacgtcgt ggagcgaaac cttgccgcgt gtcgagtgtc caagaatcgg
     2761 ggcgggagaa gaggccatgc gaacgttcct ttgcaatgga aggagggaaa tgactgctag
     2821 cgggcggtcc agccgccgtc gatcaccatc tgggatccgg tgacataggc tgcttcgtct
     2881 gacgcgagat agagcgcgag cggggcgatc tcctccgggc gacccatgcg gccgatcggc
     2941 tggcgggcgt ggagctgctt gcgcgtctcg tcctcctcgc cggcgtggta gcgatggaga
     3001 taggcat
//
LOCUS       DASTMD010000112        11633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_813909, whole genome shotgun sequence.
ACCESSION   DASTMD010000112 DASTMD010000000
VERSION     DASTMD010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11633
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813909"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VFQ54_01770"
     CDS             <1..1161
                     /locus_tag="VFQ54_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163739.1"
                     /translation="GYYPVEPGSYVYPAAPTAAMLSGVQHLATGGAVGKANIPDLARQ
                     AAEFEQVIGAPFERYFKVMLGSDPDYGNDWLGGIPSKYRAAVEDWGRKFEDATEIPQV
                     DLTGVYDDAAKELLRKMGPHGGAILGDWEELKNGYWYNVNDGSPRYGMIAKGIKVEDG
                     SYVNPDFAGTPKHYTPAPMKEDAKTIEQFLDLYREQTDGILPASAPPWDLIKNAVAWA
                     GDTRDDENKLPSQAKDVITDLTGLLNGDRADTRGLIDNLTTQEKALADLNKDGTHPGA
                     GIASDQVKRAINRLTTGTARTTLPDNAPQSYGLPERIYGSMSPSTPQPNTHRRTNTPN
                     YGTSTGNVQNYTVVNTVESDRLQQLISDSQKGRVAHDHQRLSIQARSTRTVR"
     gene            1304..3109
                     /locus_tag="VFQ54_01775"
     CDS             1304..3109
                     /locus_tag="VFQ54_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163740.1"
                     /translation="MSSPPVVPNRTYPLYTSDPITSLPVTFTATCVDATDQANTHVYA
                     AITTRNNKIITIKDMTRSGSNYSTSVRAPGTSHKINFKPVSGVPTSGYWVARLFGAQS
                     NPIPWNASASDIAATIEALPDLNEGDTLVSGRMVDPGGVTITLRGNRVGVGYLMDYNV
                     GWWATSRLKVGSTSVDIVHTIVTQGTGDIPDVGNGGHGDWYWFAFSYRAGGDGRYSGG
                     ATNPRQADRTDPWPWWYSNAAGLTLTRPDGSDPTFAGRAPLIQWAINQTNSSRIVYQE
                     VNIVDVDLGKSVWFWATAPTRPDGTSTGTDRKLCDFSGDLDDREYRTPYVLHHLHNYR
                     CGVRIFNDAGQVSFVDHTFSIVFTAASPVSSLTAFATNTGSYIDFSWPAYTVSGFDGY
                     EIRYRHASDAWGDASDTTVWSSGSTADDMALTSVRVMEFPLGVPIVFGHFVRTIAAGE
                     NLISNPREWTPPSGTLGLYGVTVISETVSPRRYVVFPYRQPRAIAVNFDIDDRVPWGQ
                     ELPIVQRSDTFFQGITCTYPLLYGNDPNVAAINDLDTVATYKQMSKSLQLKLYRDGRG
                     NMIYGHIKPWNTNEPEDQRITEISLDFIETSGAAS"
     gene            3106..4269
                     /locus_tag="VFQ54_01780"
     CDS             3106..4269
                     /locus_tag="VFQ54_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163741.1"
                     /translation="MTNVTNLRPVDAPIRPTYGAIRSRYEMWIRNSQNDANRTQRDIT
                     SQVIGATVKVDTSADIAWTLDAQIAGLNTVEPYRDWLAPYLVVEWLAPDGSLLNVRRQ
                     LGLYMVMPSDSEHTRATSVVTVDGRDPTWLLAQQTVRQRMTFGYGLDCGYVARVLFQG
                     HDTQIRLNVPNTGIYTGKVRRIAANEVALPAANEVMQAGGYWPFAADETGYITTSAAY
                     RLQQVDPRRAVYSSQGDHFDSVQLAPDKTAFKNQVTLQSVDPDATKALKDGYTARATN
                     PADKYSTVNIGTIGYTISDTSDMSLDTLKQQAEILLENRFSQWEQVSLDVVPDPRVTL
                     HECWWLDIRNDQGQTIQKPGRWRLISTEFELAANAKVRQTATLGRLATLQEIP"
     gene            4269..5039
                     /locus_tag="VFQ54_01785"
     CDS             4269..5039
                     /locus_tag="VFQ54_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163742.1"
                     /translation="MAFANDLDTMLNDATASLRKLQGLKIAQLLGRGITPDDGFGEDD
                     KIANKVAAIAYGSDTDTDVDALLRLASVNVLSKGDGLIATILGDQGQGIPILLGRIAK
                     GKYDNLELIYKRTAFATHVVPVQASQTDYIDDKDIVTTVNIPTNTNTTILMFAYAQAR
                     VPTTATQLDMRLRIGEETTFADPSTTNFSGAVGPPSSCGKDTSIQSALTVHARTFSVS
                     SDTVDVTPRNINIRWSCFGGAGNTSIRQALMFGVKVVW"
     gene            5039..7480
                     /locus_tag="VFQ54_01790"
     CDS             5039..7480
                     /locus_tag="VFQ54_01790"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163743.1"
                     /translation="MQYDLDLTQTQVGYTVGAAFNVDLLTAWLTSGGITVTSITIDPV
                     YLWIAHVDASAEPDMTDSWPAYWNQVDWQTGDPLPTPPNIDDGEPPNNTPDTTTYTLD
                     LADTTLGWTAGSDIRADQISQYLTDKGAPAQSVSLDHDGAGGLTGQIDVVRLASGATI
                     SPTDWAAYNGPASVTTYGQTSVGEPPINAADDQSPQQGTRYGDGSNASGTKSTAIGKS
                     ASASNSSTTAIGDNAQATNADAIAMGVDTTASGTEAIAIGVEALASKSATIAIGMQAQ
                     ATGSQGVAIGYQAAANTASTAMGTTAQATGISAAAYGSGAQATGVTSTAIGQGAIANN
                     SSTLALGQTALASGNLASAIGTASQALGAFSVAIGASASASGVGATAVGQAATASGGG
                     SAAYGKNAQATGTSSTAIGIASQATADFAAALGNSAVASATFALSLGTGSAGTGDHGI
                     AIGVPATTRSKYGIALGGNATVDASSPYSMAIGYSAYVPVSTPFQTIWMASDFRVDTP
                     LVASGDTAPSAESTTITLRSPDATLGTIAVTNTDGITVNGNALATASALASEASTRST
                     NDGTLATAISTETTNRTNADSTEVTNRNAAIATHAALTATHGATGAVVGTTNTQTLTN
                     KTLTAPTIADLTNMQHDHSAANKGGTLPQSSITGLVAALAAKQDDLIEKTGPRTGTST
                     QTSSGATQGTTFGSAVTQVTTADGYDPAKAYWVTISAEIFVTLASGQQAAVAPKRQDL
                     SSLGIGGFELALSTFLSEQRISSSWSFHLLANGLPGGNGVAANVLETRLQFWCSGTGT
                     INFRDGTTTVLMVPV"
     gene            7518..7763
                     /locus_tag="VFQ54_01795"
     CDS             7518..7763
                     /locus_tag="VFQ54_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163744.1"
                     /translation="MDWLEYAKVGGPGVVALVILGKGLQWLIDLILAEAKEALENAKK
                     EIKQLREDNEDLQEENDGLKDDVRELRAKLQIARRPS"
     gene            7760..7948
                     /locus_tag="VFQ54_01800"
     CDS             7760..7948
                     /locus_tag="VFQ54_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163745.1"
                     /translation="MSPIKRRVVRVLEWIAAHTSANFRFFRAEKEASIERQRLRQRPI
                     AEPTGNLWSDMYHGRDGR"
     gene            7945..8304
                     /locus_tag="VFQ54_01805"
     CDS             7945..8304
                     /locus_tag="VFQ54_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163746.1"
                     /translation="MIDIQWDAWSSSVSLLLMFLGFLSLAIILARRTRRWNGVGRAMQ
                     ALMVFAALLYAQGAYNTAIREWGSLPHIPVEVRIGTRFLAVLSVFYAIYKVSTVDLVA
                     VKNLTSRDQVDPERTHT"
     gene            8301..9995
                     /locus_tag="VFQ54_01810"
     CDS             8301..9995
                     /locus_tag="VFQ54_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU0163747.1"
                     /translation="MTNFDSQDVGPLGLGVSGFQNFDEVANKAIYDACVAMKIPADYL
                     KGIVRRESSGDWVANARTSNIRPQSGPLLPYIGVFQSAAYSRSASVPEMKPSLRDLFD
                     ACVADPKHTQAEQIMVLAGVTRSQYNEIKQQDKTYNWLNVASYHYSGNPKPSDWEDEI
                     GSGTSTSYVANIKTWWQQLDAAPQFYVPDVPATSTDTPPVTSPPVTTPATLTFGKVPF
                     PPFVDRQSVVANKPEGVGWSNLGQRHPRFVVLHRMLGTLTGTDGYFPQPGVAALTDYG
                     LGTANVDGANLDGVIYQWTNPRGYRMAWASGPVSAPYGDGLKWLNWGNAHGYDNNMPN
                     LCGVSIENSGDYNDPMTDKAFAQLAAFVAYWFDQFQVPWDKAPIGPDGISCITWHQEW
                     TIGTGKVCPGDWLMNNTNRLISAAAAIMKPYQTGTSVGETESGPSVPVTVPTPVYAKP
                     KPIDALAAFGKSNADGAKDAKAKDGTKFIWVYDDYKVIKETRRRQYADGPKYDSVPAE
                     QTVNRDTGPHLKVGEKVTILWRCTAVDDKRDYGITSYWTRVLLSDLERLTDTPQAA"
     gene            10018..10626
                     /locus_tag="VFQ54_01815"
     CDS             10018..10626
                     /locus_tag="VFQ54_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163748.1"
                     /translation="MSTKPTVVQPESVPMATEPVLTVQKQHMIVDTAAVLITGLGFKL
                     TQDQVSGAYQWIEVLWPVAYLIYTLVMNKHVGQVIRDSVWSPASVKQKVEEAIDFVHA
                     VMPGDQAVTIATPPNSATAFGLSGTPPNTDTVPTKTATPVGTPSVPVGTFIATNTPAT
                     PPAAPSEPITATDATPTTTDAPAINSETEASDGQVVRITKTA"
BASE COUNT         2385 a         3774 c         3499 g         1975 t
ORIGIN      
        1 ggctactacc ctgttgagcc ggggtcttac gtctacccgg cggcaccgac cgccgcgatg
       61 ctctccggcg tccagcacct tgccaccggt ggtgcggtag gcaaggcgaa catcccggac
      121 ctcgctcggc aagcagcgga attcgagcag gtcatcggtg cgccattcga gcgctatttc
      181 aaggtgatgc tcggcagcga tcccgattac ggcaatgact ggctcggcgg cattccgtcg
      241 aaataccgcg cggcggtcga ggactgggga cggaagttcg aggacgcgac cgagatcccg
      301 caggtcgacc tgacgggtgt ctatgacgac gctgcgaaag aattgcttcg caagatgggg
      361 ccacacggcg gtgcgattct tggcgattgg gaggaactca agaacggcta ctggtacaac
      421 gtcaacgacg gcagtccgcg gtacgggatg attgccaagg ggatcaaggt agaagacggc
      481 agctatgtga atcctgactt cgccggcacg ccgaagcatt acacccccgc gccaatgaag
      541 gaagatgcca agacgattga gcagttcctc gatctgtacc gggagcaaac agacggcatc
      601 ctcccggcct cggcgccccc gtgggacctc atcaagaacg ctgtggcgtg ggccggggac
      661 acacgggacg acgagaacaa gctgcccagc caggcgaagg acgtgatcac ggacctgacc
      721 ggcctcctca acggcgatcg cgccgacacg cgtgggctga tcgataatct cacgacccag
      781 gaaaaggccc tcgccgatct gaacaaggac gggacacatc ctggcgcagg tatcgcctcg
      841 gatcaggtca aacgggcgat caaccggctg acgaccggga cggcccggac aacgctgccc
      901 gacaacgcgc cgcaatccta tggactccct gaacggatct atggatcgat gagtccgagc
      961 acgccgcaac ccaatacgca tcgccgcacc aacacgccga attacggaac gagcacgggg
     1021 aatgttcaga actacaccgt ggtcaacacc gtcgagagcg accggttgca gcagttgatc
     1081 agcgattcgc agaagggacg cgttgcccac gatcatcagc gcctctcgat tcaggcgaga
     1141 agtacgcgaa ccgtgcgata aggcgcgcat cctccccctc gagcatgtcg gggatgctaa
     1201 aatacaggca atagcgaagg ccggccgccc gtgcgcgcag tgcccctcca gtagggacgt
     1261 tgcattcacg ggcgttcctg cgtgcaccca ctgagcgcgc cgcatgtctt cgccgcctgt
     1321 tgttccgaat cgaacgtatc cgctctacac ctccgacccg atcacgtcgt tgccggtcac
     1381 cttcacggcg acgtgtgtcg atgcgacgga tcaggcgaac acgcacgtct acgcggcgat
     1441 cacgacccga aacaacaaga tcatcacgat caaggacatg acgcgttccg ggtccaacta
     1501 cagcacgtcg gtccgggcgc cgggaacgtc gcacaagatc aacttcaagc cagtctccgg
     1561 tgtccccaca tctggctact gggtcgcccg cctcttcggc gcccaaagca acccgatccc
     1621 ctggaacgca tccgcctccg acatcgccgc gaccatcgag gccctgcccg atctcaacga
     1681 gggggacacg ctcgtcagcg gtcgcatggt cgatccgggc ggggtgacga ttacgcttcg
     1741 cgggaatcgc gtcggtgtcg gctacctgat ggactacaac gtcggttggt gggccacgtc
     1801 ccggctcaag gttggcagta cctccgtcga catcgtccac acgatcgtta cccaaggcac
     1861 cggcgacatc cccgatgtcg ggaacggcgg tcacggcgac tggtactggt tcgccttttc
     1921 gtaccgtgcc ggtggcgatg gccgctatag cggtggtgcg acgaatcccc ggcaggcaga
     1981 ccgtaccgat ccctggccct ggtggtacag caacgcggcg ggtctgaccc tgacccgccc
     2041 ggacggcagc gatcccacct tcgcggggcg tgcgccgttg atccagtggg cgatcaacca
     2101 gaccaattca tcccggatcg tctatcagga agtcaacatc gtcgatgtcg acctgggcaa
     2161 gagcgtctgg ttctgggcga cggcaccgac gcgaccggac ggaacgtcga ccggcaccga
     2221 tcgcaagctg tgcgacttca gcggcgatct ggacgatcgg gaataccgca cgccctacgt
     2281 cctgcaccac ctccacaact accgctgtgg cgtgcgcatc ttcaacgacg cgggacaggt
     2341 ctccttcgtc gatcacacct tcagcatcgt cttcactgca gccagtcccg tttccagcct
     2401 gaccgcattc gccacgaaca ccggcagcta catcgacttc agctggccgg cctacacggt
     2461 cagcgggttc gatggctacg agattcgcta ccggcacgcg agtgacgcct ggggtgatgc
     2521 gtcggacacg accgtctgga gcagcggcag taccgcggac gacatggcgt tgacttcggt
     2581 gcgggtgatg gagttcccgc tcggggttcc gatcgtcttc ggtcacttcg tgcgcacgat
     2641 cgccgccggc gagaacctga tctccaatcc ccgcgaatgg acacccccaa gcggcacgct
     2701 cgggctctac ggcgtcacgg tcatctcgga gacggtttct ccccgccgct atgtcgtctt
     2761 tccctaccgt caaccgcggg cgatcgctgt caacttcgac atcgacgacc gcgtgccgtg
     2821 ggggcaggag ttgccgatcg tgcaacgcag cgacacgttc tttcagggca ttacctgcac
     2881 gtatccgctg ctctacggca atgaccccaa cgtcgcggcg atcaacgacc tcgatacggt
     2941 cgcgacgtac aagcagatga gcaagagtct ccagctcaag ctctatcgcg atgggcgcgg
     3001 gaacatgatc tacggccaca tcaagccgtg gaacacgaac gaacccgaag accaacggat
     3061 caccgagatc tccctcgact tcatcgagac aagcggagcg gcgtcatgac caacgtgacc
     3121 aacctccgtc ctgtcgatgc cccgatccgc cccacctacg gtgcgatccg cagccgctat
     3181 gagatgtgga ttcgcaactc gcagaacgac gccaaccgga cgcagcgaga tatcaccagt
     3241 caggtcatcg gcgcgacggt caaggtcgac acctcagcgg acatcgcctg gacgctcgac
     3301 gcccagatcg cgggactgaa caccgtcgag ccctaccgcg actggttggc gccgtacctg
     3361 gtcgttgaat ggctggcgcc ggacggctcg ctcctgaacg tccgccggca actcggcctc
     3421 tatatggtga tgccatcgga ctccgaacac actcgagcga ccagcgtcgt gaccgtggat
     3481 ggacgcgatc cgacgtggct gctcgcacag cagacagtcc gccagcggat gacgttcggc
     3541 tacgggctcg attgcggcta cgtggcgcgt gtgctcttcc agggacacga cacccagatc
     3601 agactcaacg ttcccaacac cggtatctat acagggaagg tgcggcggat cgcggcgaac
     3661 gaggtcgccc tgccggcggc caatgaggtg atgcaggccg gcgggtactg gccgttcgcc
     3721 gcagatgaaa ccggatacat cacgacgtcg gctgcctacc gcctccagca ggtcgaccca
     3781 cggcgagcgg tctacagctc acagggcgac cacttcgact cggtccagct cgctcccgac
     3841 aagacggcgt tcaagaatca ggtgacgctc cagagtgtcg atcccgatgc gacgaaggcg
     3901 ttgaaggacg gctacaccgc tcgcgcgacg aatccggccg acaagtactc cacggtcaac
     3961 atcggcacga tcggctacac gatctccgac accagtgaca tgtcgctgga caccctgaag
     4021 cagcaggccg aaatcctcct cgagaaccgt ttctcccaat gggagcaggt ctcgctggat
     4081 gtcgtccccg atccgcgcgt cacgctccac gagtgctggt ggctcgacat ccggaacgat
     4141 caggggcaga ccatccagaa gcccggccgc tggcgattga tctccactga attcgaactt
     4201 gccgcgaatg ccaaggtccg acagaccgcg acccttgggc gcctcgccac gctccaggag
     4261 atcccgtaat ggccttcgcg aacgacctcg acaccatgct caacgacgcc accgcgagcc
     4321 tgcgcaagtt gcagggactc aagatcgcgc aacttctcgg gcgcggcatt acgcccgacg
     4381 atgggttcgg cgaagacgac aagatcgcga acaaggtcgc ggcgattgcc tatgggtcgg
     4441 acaccgacac cgacgttgat gcgctgctac ggcttgcctc ggtgaacgtc ctcagcaagg
     4501 gcgacgggct gattgcgacg atcctcggcg atcaggggca gggaatcccc atcctgctcg
     4561 ggcgcatcgc caaggggaag tacgacaacc tcgaactgat ctacaagcgg acagcgtttg
     4621 ccacccacgt cgtacccgtg caggcatcac agacggacta catcgacgac aaggacatcg
     4681 tgaccacggt caacatcccg accaatacca acacgacaat cctcatgttc gcgtacgctc
     4741 aagcgcgagt ccccaccacc gcgacacagc tggacatgcg tctccgtatc ggcgaggaga
     4801 ccacgtttgc cgacccctcg accaccaact tctccggtgc ggtcgggccg ccctcgtcct
     4861 gtggcaagga cacctcgatc cagtcggcgc ttacggtgca tgcccggacg ttcagcgttt
     4921 cctccgatac cgtcgatgtc acaccgcgaa acatcaacat ccgctggagc tgcttcggtg
     4981 gtgctgggaa cacgtcgatc agacaggcgc tcatgtttgg cgtgaaggtg gtgtggtgat
     5041 gcaatacgac cttgacctca cccagaccca agtcggctat accgtcggcg ccgccttcaa
     5101 cgtagatctc ctgaccgcat ggctgacgag cggcggcatc acggtcacct cgatcacgat
     5161 cgatccggtt tatctctgga ttgcgcacgt cgatgccagt gcagagccgg atatgacgga
     5221 cagttggccg gcgtattgga accaagtcga ctggcagacg ggcgatcccc tcccaacccc
     5281 gcccaacatt gatgacggcg aaccaccgaa caatacgccg gatacgacca cctataccct
     5341 cgacctcgcc gacaccacac tcgggtggac ggcgggaagt gatatccgcg ccgaccagat
     5401 cagccagtac ctcacggaca aaggcgctcc cgcccagtcg gtctccctcg atcacgatgg
     5461 tgccggtggg ttgaccggcc aaatcgacgt ggtgcggctt gccagcggcg cgactatttc
     5521 gccaaccgac tgggctgcct ataacggtcc ggcgagcgtc acaacgtacg gtcagacctc
     5581 ggtcggtgag cctccgatca acgctgccga cgatcagtct ccgcagcagg gcactcggta
     5641 cggcgatggc tccaacgcga gcggcaccaa gtcgacggcg atcggcaaga gcgccagcgc
     5701 ctctaactcc agcacgacgg cgatcggtga caacgcgcag gcgactaatg ccgatgccat
     5761 cgcgatgggc gtcgacacca ccgcgagtgg cacggaggcg atcgcgatcg gcgtggaagc
     5821 actcgcctcg aagagcgcga cgatcgcaat cggcatgcag gcgcaggcga ccggctcgca
     5881 gggtgtggcg atcggctacc aggccgcggc gaataccgca tcgacggcga tggggacgac
     5941 ggcgcaggcg accgggattt ccgctgcggc gtacgggagc ggcgcccagg cgaccggcgt
     6001 cacgtccacc gccatcggtc agggggcgat cgcgaacaac agctcgaccc tcgcgcttgg
     6061 tcagacggca ctcgcaagcg ggaacctggc gagcgccatc ggtacggcat ctcaggcatt
     6121 gggcgccttc tcggtggcga tcggcgcatc ggcaagtgcg tccggtgttg gggcgacggc
     6181 tgtcggtcaa gcggctaccg cgtccggcgg agggagtgct gcctatggca aaaacgccca
     6241 agccaccggg acgtcctcga cggccatcgg gatcgcatct caagcaacgg cggacttcgc
     6301 agcggcgttg gggaacagcg ccgttgcgag cgcgaccttt gccctgagcc tcgggacggg
     6361 atcggccgga acaggagatc acggaattgc gatcggtgtt ccggcgacga cacgttcgaa
     6421 gtacggaatt gcgctcggtg ggaatgcgac ggtcgacgca agttccccgt attcgatggc
     6481 cattgggtat agcgcgtatg tgcctgtgag cacgccgttc cagacgatct ggatggcctc
     6541 tgacttccgg gtcgatacac cgctggtggc atccggagat accgcgccca gtgcagagtc
     6601 gacgacgatc acgttgcgat cccctgatgc gacactcgga acgattgcgg tcacgaacac
     6661 ggacggaatc accgtcaatg gcaatgccct cgcgacggca tccgcgctgg cgtccgaggc
     6721 gtccacgcgc tcgaccaatg acgggacgct cgcaacggcg atctcgaccg aaaccaccaa
     6781 ccggaccaat gcggacagca ccgaggtcac aaaccgcaat gccgccatcg ccacgcatgc
     6841 ggcgctgacg gcgacccatg gggctaccgg cgccgtcgtg ggcacgacca atacccagac
     6901 actcaccaac aagacgctga ccgcgccgac gatcgccgat ctcacgaaca tgcagcacga
     6961 ccattcggcg gccaacaagg gcggcactct cccccagagt tcgatcaccg gactggtcgc
     7021 ggcgctcgcg gccaagcaag acgacctgat cgagaagacc gggcctcgta ccggaacgag
     7081 cacacagacc tcgtccggag cgacgcaagg cacgacgttc ggttccgcgg tgacgcaggt
     7141 gacgaccgcc gatggctacg acccggcgaa agcgtactgg gtcacgatct cggccgagat
     7201 cttcgtcacg ctcgccagtg gccagcaagc cgccgttgcc ccgaaacgcc aggacctttc
     7261 atccctcggc atcggcgggt tcgaactggc gctgagcacc ttcctttccg agcaacgcat
     7321 ttcgtcctcg tggtcgttcc acctgctcgc caacggcttg ccgggtggca acggtgtggc
     7381 tgcgaatgtc ctcgaaacgc gcttgcagtt ctggtgctcc ggcaccggca ccatcaattt
     7441 ccgcgatggg acaaccacgg tgctcatggt gccggtgtga tgaaggagac cgcagatgta
     7501 tcaggtggat atcagtgatg gattggctgg agtacgcgaa ggtcggtggt cccggagtcg
     7561 tagccctggt gatcctgggc aaggggctgc agtggctcat cgatctcatc ctcgccgaag
     7621 cgaaggaagc gctcgagaac gccaagaagg aaatcaagca actgcgcgag gacaacgagg
     7681 acctgcaaga ggagaacgac gggctcaagg acgacgttcg cgagctccgc gccaagctgc
     7741 agattgcgag gcgaccatca tgagcccgat caagcggcgc gtggtgcgcg tcctcgaatg
     7801 gatcgccgcg catacctccg ccaacttccg gttcttccgc gccgaaaagg aagcgtcgat
     7861 cgaacgccag cgtctccgac aacggccgat cgccgagccg accggcaatc tctggtcgga
     7921 catgtatcac gggagggacg gcagatgatc gatatccaat gggacgcctg gagcagttcc
     7981 gtctccctcc tgctgatgtt cctcggcttc ctgtcgctgg cgatcatcct cgcgcgacgg
     8041 acgcgccgct ggaacggggt ggggcgggca atgcaggcat tgatggtctt cgccgcgctg
     8101 ctctatgccc agggcgccta caacacggcg attcgcgagt ggggcagtct gccgcacatc
     8161 ccggtcgagg tgcggatcgg gactcgcttc ctcgccgtgc tctccgtctt ctacgccatc
     8221 tacaaggtca gcaccgtcga tctcgtcgcc gtcaagaacc tgacctcgcg tgatcaggtc
     8281 gaccccgaaa ggacacacac atgaccaatt tcgacagtca ggacgtcggc ccgctcgggc
     8341 tcggcgtctc cggtttccag aatttcgacg aggtcgcgaa caaggcgatc tacgacgcat
     8401 gtgtcgcgat gaagatcccg gcggactacc tcaagggtat cgtccggcgc gagtcctctg
     8461 gtgactgggt ggcgaacgca cggacgtcca acatccgtcc ccagagcggt ccgctcctgc
     8521 cgtacattgg cgtctttcag tctgcggcgt attcccggag cgcttccgtg ccggagatga
     8581 agcccagtct tcgtgacctg tttgacgcct gtgtggccga tccgaagcac acgcaggcag
     8641 agcagatcat ggttctcgct ggtgttaccc gcagccagta caacgagatc aagcagcaag
     8701 acaagaccta taactggctg aacgtcgcgt cctatcacta ttcggggaac ccgaaaccta
     8761 gcgactggga ggacgagatc gggtccggca cgagcaccag ctacgttgcg aacatcaaga
     8821 cctggtggca gcaactcgac gcggcgccgc agttctatgt cccggacgtg ccggcgactt
     8881 caacggatac accgccagtg acgtcgccgc cggtcaccac gccggcgacc ctcacgttcg
     8941 gaaaggtacc gttccctccg ttcgtggatc gtcagtccgt tgtcgcgaat aagccggaag
     9001 gagttggttg gtcgaatctc ggtcagcgcc atccgcgctt cgtggtgctg caccggatgc
     9061 tgggaaccct caccgggacc gatggctact tcccgcaacc cggcgtagcc gccctcaccg
     9121 actacggact cgggaccgcg aacgtcgacg gcgccaatct ggatggggtg atctatcaat
     9181 ggacgaaccc ccgcggatac cggatggcgt gggcgtcggg tcctgtcagc gcgccgtatg
     9241 gcgacggact caagtggctc aactggggaa acgcgcacgg gtatgacaac aacatgccga
     9301 acctgtgcgg ggtctcgatc gagaactccg gggactacaa cgatccgatg acggacaagg
     9361 cgtttgctca gctcgccgcg ttcgtcgcct actggttcga tcagttccag gtgccatggg
     9421 ataaggcgcc gatcggtccg gacggtattt cctgcatcac ctggcatcag gagtggacca
     9481 tcggcaccgg caaagtctgc cccggtgact ggctgatgaa caatacgaat cggctcattt
     9541 ccgcagcggc cgcgatcatg aagccgtacc aaaccggaac gtctgtcggt gaaacggaaa
     9601 gcggaccatc tgttcccgtc accgtaccga cgccggtcta tgcgaagccc aagccgatag
     9661 atgcgctcgc ggcgttcggc aagagcaacg ccgatggtgc caaggacgca aaagccaagg
     9721 acggcacgaa gttcatctgg gtctatgacg actacaaggt gatcaaggag acacggcggc
     9781 ggcaatacgc ggacggtccg aagtacgaca gcgtgcccgc ggaacagaca gtcaaccgtg
     9841 acaccggtcc acacctgaag gttggcgaga aggtgacgat cctgtggcgg tgcaccgccg
     9901 tcgacgacaa gcgcgactac ggcatcacgt cgtactggac gcgcgttctg ctctccgatc
     9961 tcgagcgcct caccgataca ccgcaagcgg cctgatccgc acccataagg agttctcatg
    10021 agcaccaaac ccaccgtcgt gcagccggag agcgtgccga tggccaccga acccgtcctg
    10081 accgtgcaga agcagcacat gatcgtcgat accgcggccg tgctgatcac cggcctcggc
    10141 ttcaagctca cccaggatca ggtgagcggc gcgtatcagt ggatcgaagt cctctggcca
    10201 gttgcctacc tgatctacac gctcgtcatg aacaagcatg tcggccaggt gatccgcgat
    10261 tcggtctggt cgccagccag cgtcaagcag aaggtcgaag aagcaatcga cttcgttcac
    10321 gccgtcatgc cgggcgatca ggcggtcact atcgcgacgc ccccgaacag cgcgacggca
    10381 tttggactct cggggacgcc cccgaatacg gataccgtcc ccacgaagac cgccacacct
    10441 gtcgggacgc ccagtgttcc tgttggaacg ttcatcgcaa cgaacacacc cgcgacgccg
    10501 ccggcagcac cgtccgagcc catcacagcc accgacgcta cgccaaccac gaccgacgca
    10561 cctgcgatca actcggagac ggaagcgtcg gacggccagg tcgtccgcat caccaagacg
    10621 gcgtagtcga aacaagaggg cgggagacac agaaagcccg ggcgccgttg cgcccgggct
    10681 ctctcttaac ttctgataaa caacacccgt tcgcgatccc cattctcatc ctcgatgatg
    10741 agcttggatc gacctcgctt cgcctcacga aggaactgct cggtctcgat cgcatctcgg
    10801 acgaccctcg tcactgaggt attccgttcc ttcgccatcg acttgatcgt cgcagcaacc
    10861 tcggccggaa ccttcaatga caaacgcaca ggctccgctg cctcgcgctg ctctgccgtc
    10921 ctattttcca tgggtttgcc cttctatgta ggtagttatc tccgacaacg atctccgctc
    10981 cttgatcggc attatgcact catagtacgc acaatgtgcg ccccgagatg ctgatcgtgt
    11041 acactttcga gtcgtgctcc gcgcacgaac aagggccaga ggttgcaccc cctgaccctt
    11101 gcacgaaggc tcacgcgaat gggcctagct tcccttcttc accgggacgt gctgaacgac
    11161 cgtcgtcttt ggatgcttcg ctgcgtaact gggtttgaca aacttcccag ttaccgcgct
    11221 gcgtgcaatg gtgacggtct tcgtgctgtt gctcatagcg gcaggtacct ccttcccgta
    11281 ggattcgcct tttggcgtgt gtatatagta caccaaaagt gcgcacctgt gagaacgatg
    11341 aggcgtgagc gaagacatag ccgtctgcgc gatatactga gagcaatcca gttacctgga
    11401 ggtggagtgg cgaaagccct cgcccaagac gagcagtcca tcgtcttttc gattgctgaa
    11461 gtccctgcct gctgtggtgg aggggcggta cccaatccgc ctaggctttc ggttctggtg
    11521 gcaacaagag actccggtga gtggaatgct caccggagtc tcttgcgaat gtgcgatttc
    11581 acgagcggct agagggcggg ggagacggag tgtcgggcag aacatccgtc cta
//
LOCUS       DASTMD010000113         1790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_991994, whole genome shotgun sequence.
ACCESSION   DASTMD010000113 DASTMD010000000
VERSION     DASTMD010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991994"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..798
                     /locus_tag="VFQ54_01820"
     CDS             430..798
                     /locus_tag="VFQ54_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163749.1"
                     /translation="MPRPISIDAFGSQENVRPRTSPGASVVIPFVVQRGAQLFLLAAE
                     AAEWVLAELRFDETTCTYAELRKVRYEWPREAFGALLSRVAVAGDVDSDLVEETSEEF
                     SRWLGLQFQLSAADFPEPDA"
     gene            complement(1110..1658)
                     /locus_tag="VFQ54_01825"
     CDS             complement(1110..1658)
                     /locus_tag="VFQ54_01825"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEU0163750.1"
                     /translation="MAIETGRLGGYRERKDTYFKDHENSPLTPDQKEIFNGLSYFPEN
                     ENLSCTLEVDEIGEGVGEVVTIATVSGVEKQYVRVGRVHFPVNGEDTTLSVFQDTKSG
                     KFFLPFRDGTSGKETYAVGRYLDPKATPDGKLVVDFNLAYNPMCAYNEGWACPIPPFE
                     NFLKVPIHAGEVLPTFVDHSDH"
BASE COUNT          327 a          540 c          577 g          346 t
ORIGIN      
        1 gtggttgccc ggatatcgac gatcttcacg cgggctggat cctttcctga agcaacgggc
       61 cggcggcccc gaccggggac gcggagggga gggcaggagc gggcgtatgc atgaagagat
      121 ctaatgactt gatgagtcca ttgtcaatga cctgcggctg ttcgaagccg ccgttcgccg
      181 cgagcctcat cgggcggttt tgccttgaag gggctttcgc aatgatgcta cgctgacctc
      241 tgaacacgtc ggccttaccc tgacgaactc ccccattttc acggggtgtc gcaacgccgg
      301 aaatgcccgg tatgtcgaca gggtgtggtc gagaaggatt gctcgaccat gtgccagccc
      361 cgctcccacg aacaagcaga tgaatgccgg atgtccagcg gctgtggcga tgggcagatg
      421 acgaggcatg tgccgcgccc gatttcgatc gatgcgttcg gttcgcagga gaacgttcgc
      481 ccaaggactt cgccgggggc gagcgtggtg atcccgttcg tggtgcagcg gggggcgcaa
      541 ctcttcctgc tggcggcgga agcggcggag tgggtattgg ccgaactccg atttgacgag
      601 acgacctgca cctatgccga gctccgcaag gttcgctacg aatggccgcg tgaggccttc
      661 ggcgcgctcc tgagccgggt tgcggtggcc ggagatgtcg acagcgatct cgtcgaggag
      721 acgagcgagg agttctcgcg gtggctgggg ctgcagttcc agctcagcgc cgccgatttc
      781 cccgagcctg acgcctagtc ctggggcggg acccagtctc gaggcgggac atagaagtcc
      841 cgccgtactc acgaacgctg cccgtgtggc ctcgccaggc gataccctcg catccgggat
      901 gcggggtctg tagggcggcg agggggcatc tctcgccgca agcccaacgt caatgcacga
      961 cgcctccgcc aactaatcgc tcgcgaacgt tcggctccgc tgcgtcgatc cgaaagggcg
     1021 acgagcgcca cggggacggg aatgggcagg acgcaagaca cccccggagc aaacgctccg
     1081 ggggtgtctg agttaccgat tatcgaatct caatggtccg agtgatcgac gaacgtcggg
     1141 agaacctcgc cggcgtggat cggcaccttc aggaagttct cgaacggcgg gatcgggcac
     1201 gcccatccct cgttgtacgc acacatcggg ttgtaggcga gattgaagtc caccaccagc
     1261 ttgccgtccg gtgtcgcctt cgggtcgagg taacgaccca ccgcgtaggt ctccttgccg
     1321 gaggtcccgt cgcggaatgg caggaagaac ttgccgctct tcgtgtcctg gaagacgctc
     1381 agcgtcgtgt cctcgccgtt gaccgggaag tggacgcgac cgacgcgcac gtactgcttc
     1441 tcgacaccgc tcaccgtcgc gatcgtgacg acctcaccga cgccttcgcc gatctcatcg
     1501 acctccagcg tgcagctgag gttctcgttc tcgggaaagt aggaaagacc attgaagatc
     1561 tccttctgat cgggagtcag cggggagttt tcatgatcct tgaagtacgt atccttgcgc
     1621 tcgcgataac cgccgagccg ccctgtttca atcgccatag gtatcatccc cctcgtgatc
     1681 cggcgaggct tcctgccccg cctttaccat tactttactt tgtatagtat agaacatctg
     1741 tcaaggtcga gacagctgga tcgagagacg aaagaggcgc tccctgcggt
//
LOCUS       DASTMD010000114         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2867578, whole genome shotgun sequence.
ACCESSION   DASTMD010000114 DASTMD010000000
VERSION     DASTMD010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2867578"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFQ54_01830"
     CDS             <1..244
                     /locus_tag="VFQ54_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163751.1"
                     /translation="HPAQRDGRLGGGDVFDGHCGPPCPRDVSGVAMGDDAKRMRAHPI
                     TRNPRIDSLFIASVNPWLMIPDATFRILPQMDRWPS"
     gene            305..1057
                     /locus_tag="VFQ54_01835"
     CDS             305..1057
                     /locus_tag="VFQ54_01835"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU0163752.1"
                     /translation="MRVIAHRGASTEFPENTPGAFRRAIELKPFGTELDIHLSADGVA
                     VVIHDRTVDRTTNGHGDVSSLTLAELKTLDAGNGEQIPTLDEVIALVGDKLHLFLEVK
                     DANTIELVLATMQRHPSVSWTMLSGDPAPVQGVRRRYPGVELCVGAGRLRGDLDAGAR
                     ATQLESAIASAREVSAAILMVHDSAVDDTVVRRVQEAGFAIWAWTVNDVPRMAELARM
                     GVDYLCTDDAGSAIAGWSEPTSVLAGKHTQTG"
     gene            1081..>1648
                     /locus_tag="VFQ54_01840"
     CDS             1081..>1648
                     /locus_tag="VFQ54_01840"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU0163753.1"
                     /translation="MRVYAHRGASIERPENTLSAFRRALELKPAGIELDLQLSRDGVP
                     VVIHDESVDRTTNGSSPVSSFTVAELRELDAGDGEYIPTLREVMDLVASTPSSCRARM
                     ILEVKNEDVVPALLAEVSSRADVDWSILSLNWEALRDIRAIDANADLWMNTFGTSDVL
                     RIFQPESPSLRMLDEALEVLREIKASTLA"
BASE COUNT          289 a          508 c          528 g          323 t
ORIGIN      
        1 acatcctgcc caacgcgatg gtcgtttggg tggtggtgat gtctttgatg gtcattgcgg
       61 gcctccctgt ccacgcgacg tgagtggtgt cgcgatgggt gatgatgcga aacgaatgcg
      121 tgctcatcct atcacgcgaa atccgcgcat tgatagcctt ttcattgcgt ccgtcaatcc
      181 atggttgatg attccggacg caacgttccg catactcccc cagatggacc gctggccgag
      241 ttgatcgttg gggcacgtgc cgccacggtc ccggacccag gatcgttcac gggagttgtc
      301 gttcatgcga gttatcgcgc atcgtggtgc gtcgaccgag tttccggaga atacccctgg
      361 cgcctttcgc cgcgcaatcg agctgaagcc gttcggcacc gaactcgata tccacctctc
      421 cgccgatggc gtcgccgtcg tcattcacga caggacggtc gatcgcacca ccaacggtca
      481 cggcgatgtc tcgtcgttga ccctggccga gctgaagacg ctcgatgccg ggaacggcga
      541 gcagatcccg acgctcgacg aggtgatcgc gctggtcggc gacaagcttc atctcttcct
      601 cgaagtgaaa gatgccaaca cgatcgagct cgtgctcgcg acgatgcagc ggcatccgag
      661 tgtgagctgg acgatgctct cgggagatcc cgccccggtg cagggcgtgc ggcgccggta
      721 tccgggtgtc gaactctgcg tgggagcggg ccgcctgcgc ggggatctcg atgccggagc
      781 acgcgcgacg caactggaat ccgcgatcgc atcggcgcgc gaggtcagcg cggcgatcct
      841 gatggtccac gacagtgctg tcgacgatac ggtggtgcga cgtgtccagg aggctggatt
      901 cgcgatctgg gcgtggacgg tgaacgacgt cccgcggatg gcggaactgg cccggatggg
      961 cgtcgattat ctctgcaccg atgatgcagg gagcgccatc gccgggtggt cggagccgac
     1021 ctccgttctt gcagggaagc acactcaaac tggatagcaa tctcctgggg tgatttcaac
     1081 atgcgtgtct acgctcatcg cggtgcctcg atcgaacgac cggagaacac gctctccgca
     1141 tttcgacggg cgttggaact gaaaccggct ggcatcgaac tcgacctgca actctcccgg
     1201 gatggtgttc ccgtcgtcat ccacgacgaa tcagtcgatc gcacgaccaa cggcagtagc
     1261 cccgtctcat ccttcaccgt cgccgaactg cgggaactcg acgccgggga cggggagtac
     1321 atcccgaccc ttcgcgaggt gatggatctc gtcgcatcga ccccgtcgtc gtgtcgggcg
     1381 cggatgattc tcgaagtcaa gaacgaggat gtcgtaccag cgctgctcgc cgaggtctcg
     1441 tcgcgggcgg acgtcgactg gtcgatcctc tcgctcaact gggaggcgct ccgcgacatc
     1501 cgcgcgatcg atgcgaatgc cgatctctgg atgaacacgt tcgggacatc ggacgtgctc
     1561 cgtatctttc agccggagtc gccgtcgctc cggatgctcg acgaggcgct cgaggtcctg
     1621 cgcgagatca aggcttcgac cctcgcgc
//
LOCUS       DASTMD010000115         1811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2956968, whole genome shotgun sequence.
ACCESSION   DASTMD010000115 DASTMD010000000
VERSION     DASTMD010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2956968"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..676)
                     /locus_tag="VFQ54_01845"
     CDS             complement(350..676)
                     /locus_tag="VFQ54_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163754.1"
                     /translation="MNDATRDSIEMIAGVPLVLVLPALVELVKQQGVPARWAGISSIA
                     IATFLVAVGDLALGNSGEPGWLVRAAIWIVQGIVYGLAAAGLYSQRELILPGQKTKAP
                     PTSAKS"
     gene            complement(673..1032)
                     /locus_tag="VFQ54_01850"
     CDS             complement(673..1032)
                     /locus_tag="VFQ54_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163755.1"
                     /translation="MNEQPVRVIAGDGSDTPDDPFPPPETTRDLRESRQVVVTVPLLR
                     ARRSADTTQPIVAEWPRGHRFWVRGWIIGETVEQNPVWWLTGKGKPTDRRWRVWSGGT
                     SIAGRDVLALPVAGGDA"
     gene            1223..1486
                     /locus_tag="VFQ54_01855"
     CDS             1223..1486
                     /locus_tag="VFQ54_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163756.1"
                     /translation="MVHVEGPGGDEMFPRERGDLNDHEQRALDGFRCPTCGSPLDLFE
                     RQREAAPGTTPGDDECCELMAACRQCMLTFTKEDWQARGSLPS"
BASE COUNT          324 a          588 c          585 g          314 t
ORIGIN      
        1 gccggtccgg atctgcgatc tgaaagccgc ggcaatgccc cgccccacgc acgcggtgag
       61 agacaccgca tgaatactgg catcttgcga tgcaacaccg tcggaacgac ctggggtttg
      121 tagggcggga gcttgtctct cgccgcgacg cgaacgtcac cctcacgatg tcttgcgatc
      181 accgatcctc gtgacaacga atcgacgcag cgacccggaa gcggccacaa gggccgcggg
      241 gacgggtttc gtcggcacgt gaacggcgag gcatcgatcg tcacaacgca ggagaggcgt
      301 cccggtcagt gccgggacgc ctctcctgat ttcagattca cgcaatcgct catgatttgg
      361 ccgaggtcgg cggtgccttc gtcttctgtc ccggcaggat gagttctcgt tgcgaataga
      421 gtcccgccgc cgcaagcccg tagacgattc cctgcacgat ccagatcgcg gcgcggacga
      481 gccatccggg ttcacccgaa ttccccaacg ccagatcgcc caccgccacg aggaaggtcg
      541 cgatcgcaat cgaggagata ccggcccagc gagccggaac gccttgctgc ttcacgagtt
      601 cgacgagggc gggaaggacg agcaccaggg gaactccggc aatcatttcg atcgagtcac
      661 gtgtcgcgtc gttcatgcgt cacctcccgc gacgggcagg gcgaggacat cacgaccggc
      721 gatgctcgtg ccgcctgacc agacccgcca gcggcgatcg gtcggcttgc ccttccccgt
      781 cagccaccag accggattct gctcgacggt ttctccgatg atccagccgc ggacccagaa
      841 gcggtgaccc cgcggccatt cggcgacgat cggttgcgtc gtgtccgccg atcgtcgcgc
      901 cctgagcagc ggcaccgtga cgacgacctg ccggctttcg cgaaggtcgc gggtcgtctc
      961 gggcggcgga aagggatcgt ctggagtgtc cgatccgtcg cccgcgatga ctcggacggg
     1021 ctgctcgttc attttggact tgtgatttcg cagtgtatga tccatttgag atatctccta
     1081 tggaagaagt ttgcgggagg cgggcagcgt gaggatcgca tgatccggca ccgtggacga
     1141 tcgacccggg gaatgccaga tttcgtgtaa tactggaatc gtggcgatgg gcgagcgtca
     1201 aatgaacacg aggaggtcac cgatggttca cgtcgaaggc cccgggggcg atgaaatgtt
     1261 cccgcgcgaa cgtggggacc tgaacgatca cgaacagcgc gctctggacg gattccgctg
     1321 tcctacctgt gggtcaccac tcgatctctt cgagcgccag cgcgaagctg cccccggcac
     1381 gacaccgggc gacgacgaat gctgcgaact gatggccgcc tgtcgccagt gcatgctcac
     1441 attcacgaag gaagattggc aggcgcgggg atcgctccca tcgtgacgct tgcctgcggc
     1501 gaaaaacgga acgatttcca catactgccg ggtcgggctc gacgtcccgc acggcatccg
     1561 gtcgttcgta gcgcgggacc gccgctgccg gaccgcagtc cggacggcct gtcccgcgtc
     1621 gcgttcgtcc ggggatcgct cccatcgtga cgctcgccta gacctcgacg agggtcagca
     1681 tgaccgtcgc gtctccccag cgaccggcat cggcgggccg cgccaccttc tccgtcatcc
     1741 cgacgatccg gacccggcgt tccgccggtg cggcgtcgta gtcgagatcg cgaaaggtga
     1801 gcgtcgtgcc g
//
LOCUS       DASTMD010000116         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1349277, whole genome shotgun sequence.
ACCESSION   DASTMD010000116 DASTMD010000000
VERSION     DASTMD010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349277"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..1269
                     /locus_tag="VFQ54_01860"
     CDS             337..1269
                     /locus_tag="VFQ54_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0163757.1"
                     /translation="MEQRRLGTSGLVVSTIGLGGNTFGATVDGDEAVKTIQHAIEQGI
                     TFIDSADVYSQGRSEELVGQAVQGIRSSVVIATKMGNQMNSGPHAVGLSRRWVHQAVE
                     DSLRRLNTDYIDLFYAHRPDPHTPLEETLRAFDDLVRSGKVRYIGCSNYPAWEVAYGV
                     GISKELHLDRWVAAQNRWNLIDGLDSPELEQAADFLGVGIIPFTPLASGILTGKYKAG
                     EEPQAGTRFADLAGLRGRLTAEKVEKAARVQKWAEERGHTAAEAAIAWLLAHPVVSSV
                     IAGARNPEQLTANIAAGEWTLTPEERDELSALVA"
     gene            complement(1709..>2131)
                     /locus_tag="VFQ54_01865"
     CDS             complement(1709..>2131)
                     /locus_tag="VFQ54_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU0163758.1"
                     /translation="WLTIRGLKTLAVRMDRHCQNATEIAHFLEGHPAVKRVFYPGLES
                     HPGHEIACRQMKAFSGMISFEVNGGFEKAKDVVERTELFLLAESLGGVESLIEHPGKM
                     THISVEGTGVSVNHDLIRMSVGIENVQDLIADLEQALA"
BASE COUNT          383 a          690 c          667 g          391 t
ORIGIN      
        1 gagatcccag tccttgttga tatcagtcga cgtgaggttg agtggcttgc ccgcaccggg
       61 aagattgtcg aactcgccac gatcgcgcgc ctcgcggagt tccttctcga tccacgaatc
      121 ccaatcgtga atctcgaccc gggcgtcgcc ttcgtgtcgg atgggcattt ctttcctcca
      181 cttcgttgtg ggtggtcgac tgactgcacc gcattgtgaa ccttttcacg attctagcat
      241 ggtgggtcaa gacttgcgcc atttccgttt cgggacatac tgcaaaaagg aaagcgctcg
      301 cgagagcgcg gcgtatcgag cctgacagga gaacagatgg aacagcgacg attgggaacc
      361 agcggattgg tcgtctcgac gatcggcctt ggcggcaaca cgttcggggc aacggttgac
      421 ggcgacgagg cggtaaagac gattcagcac gcgatcgagc agggaattac gttcatcgat
      481 agcgccgacg tctactcgca gggacgctcg gaagaactgg tcgggcaggc ggtccagggg
      541 attcgctcct cggtcgtcat cgcgacgaag atgggcaacc agatgaacag cggcccccac
      601 gcggtcggac tctcccgccg ctgggttcac caggccgtcg aggattcact ccgccgcctg
      661 aacacggact acatcgatct cttctacgcc catcgtccgg atccgcacac gccgctcgag
      721 gagacactgc gggcattcga cgatctcgtc cgctcgggca aggtgcgcta catcggctgc
      781 tcgaactacc cggcgtggga agtcgcatac ggcgtcggca tctccaagga gctgcatctc
      841 gatcgctggg tcgcggcgca gaaccgttgg aacctgatcg acggtctgga cagcccggag
      901 ctggagcagg cggccgactt cctcggcgtc ggcatcattc ccttcactcc gctcgcatcc
      961 ggcattctga ccggcaagta caaggccggg gaggaaccgc aagcgggcac ccgcttcgcc
     1021 gatctcgccg gcctccgggg tcgcctgacc gcggagaagg tcgagaaggc cgcccgggtc
     1081 cagaagtggg cggaggaacg cggtcacacg gcagcggaag ccgcgatcgc ctggctgctc
     1141 gcgcatccgg tcgtctcgtc cgtgatcgcc ggcgcccgca accccgagca gctgacggcc
     1201 aacatcgccg ccggtgagtg gacgctgaca cccgaagagc gcgacgaact ctccgccttg
     1261 gtcgcgtagg ttcggcacgg tcgtcgttcg cgggatgtcc ggaatagacc gttcgtcggg
     1321 gcggacctgg tgtccgcccc tacggaatct cgtactgtac gggaacctct cgacagaatg
     1381 cggcaggact tatgccaagc cgcatcgtgg tgcatccacc aagtcccggg cgcgatggcg
     1441 tgacctcaaa ggcgggacat agaagtcccg ccctacgaac ggacgctggg cgtatgccgg
     1501 tcgtccgtag ggtgggactt ttatgtcccg ccttctgaag cggcataagc gcagtcaaca
     1561 ccttggggga ccgcgtgtgt attctgctgt gcgcccggtc gttcgtaggg cgggaccgcc
     1621 gctgccggac cgcaggtccg gacggcctgt cccgccttcc gttcgcccac ggatcgtgct
     1681 ccccgtcgcg acgactccgg atctggcgct aggccagcgc ctgctcgaga tcggcgatca
     1741 ggtcctggac gttctcgatt cccaccgaca tccggatcag gtcgtgattc acgctgacac
     1801 cggtgccctc aacgctgatg tgcgtcatct tccccggatg ctcgatcagc gactcgacac
     1861 cgccgaggct ctccgcgagc aggaagagtt cggttcgctc gacgacgtcc ttcgccttct
     1921 cgaagccccc attgacctcg aacgagatca ttcccgaaaa cgccttcatc tggcggcagg
     1981 cgatctcgtg accgggatgg ctctccagac cgggatagaa cacccgcttc acggcgggat
     2041 ggccctcgag gaaatgcgcg atctcggtcg cgttctggca gtgccgatcc atccgcaccg
     2101 cgagcgtctt caacccgcga atcgtcagcc a
//
LOCUS       DASTMD010000117         1868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1974974, whole genome shotgun sequence.
ACCESSION   DASTMD010000117 DASTMD010000000
VERSION     DASTMD010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974974"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..989
                     /gene="nadC"
                     /locus_tag="VFQ54_01870"
     CDS             108..989
                     /gene="nadC"
                     /locus_tag="VFQ54_01870"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU0163759.1"
                     /translation="MTNAPILTAPRHFGLDQIVDLALQEDIGTGDVTTLATVPADRHA
                     TGTILAKEAGVISGIEVARFVFARVDSAIEFTALVSDGDRVEVRTDLARIAGPARSLL
                     VAERTALNLMQRLSGIATIAARYVAATEGTKAKIVDTRKTTPGLRELEKRAVQHGGAR
                     NHRFGLADGILIKDNHLAAIGGPDRITKAIVAARDFAPHTLRVEIEVTNLAELDEALQ
                     AGADIILLDNMDTPTMAVAVMRRDAVGSAALLEASGGITLGRIPEIAAAGVDLISVGA
                     LTHSAPALDISLDLNLS"
     gene            complement(1260..>1868)
                     /locus_tag="VFQ54_01875"
     CDS             complement(1260..>1868)
                     /locus_tag="VFQ54_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163760.1"
                     /translation="QNAYLIETPEGNILWDLVALIDEATIAEIEARGGIKAIAISHAH
                     YYTTMVEWSHALGHVPIFLHDSNRQWVMRPDSVVEYWSGDTLKPIPGITVIRTGGHFP
                     GGMVLHWSGTPDGRGALFTGDIVKVVADPNWVTFMYSYPNTIPLDPATVARMATQLAR
                     FRFDRVHDAFDKHVATDGNRAIQRSAERYISHVLGHAGPTIG"
BASE COUNT          311 a          622 c          610 g          325 t
ORIGIN      
        1 agcactacga gaacggcccg gccaacctgc tcagccagcg aaagcgcgat cctgacgcga
       61 tcgttggcga atcctgattt ccgaccccac cccggagacc catcaccgtg accaacgcgc
      121 ccatcctgac tgctccgcgt cacttcggcc tcgaccagat cgtcgatctc gccttgcagg
      181 aggacatcgg gacgggtgac gtcacgaccc ttgcaacagt ccccgcggat cgtcatgcga
      241 ccgggacgat tctcgcgaag gaagccggcg tgatctcggg gatcgaggtc gcgcgtttcg
      301 tcttcgcccg tgtggacagc gcgatcgagt tcacggcgct cgtcagtgat ggcgatcggg
      361 tcgaagtgcg gaccgatctc gcccggatcg ccggtccggc gcggagtctc ctcgtcgcgg
      421 agcggaccgc gctcaacctg atgcagcggc tctccggcat cgccacgatt gccgcgcgct
      481 acgtcgcggc gaccgagggg acgaaggcga agatcgtcga cacgcgcaag acgacgccgg
      541 gtctccgtga gctggagaag cgggcggtcc agcacggtgg cgcccgcaat caccgcttcg
      601 gtctggccga cggcatcctg atcaaggaca accacctcgc ggcgatcggc ggtccggacc
      661 ggatcacgaa ggcgatcgtg gcagcgcgtg acttcgcgcc ccacaccctg cgggtcgaga
      721 tcgaggtcac gaacctcgcc gaactcgacg aggccctcca ggccggcgcc gacatcatcc
      781 tgctcgacaa catggacacc ccgacgatgg cggtcgccgt gatgcgtcgt gatgccgtgg
      841 gaagcgcggc gctgctcgaa gcgtccggtg ggatcacgtt gggtcggatc cccgagatcg
      901 cggcggccgg ggtcgatctg atctcggtcg gcgcactcac gcattccgcc ccggcgctgg
      961 atatcagtct cgatctcaac ctttcctagt ggtctgtcac cggtgttttg ccggttggca
     1021 tcgtctgcca acgatcatat gcggcttcgc cgcatgggcc acacggggtg gcccgctacg
     1081 agatcaccgt ggcaatccag ctgtgacgct ccattagaac gagaagcgtc tggtgagacg
     1141 acgacggcca ggcgccgtga gcgggagacc gcttcgcggc gtattgaatg cggcggtcgt
     1201 gttcgaggcg ggacttctat gtcccgcctc gaatcgcgtc ccgcctcgaa ccgctgcggt
     1261 cagccgatcg tcgggccggc gtggccgagg acgtggctga tgtagcgctc cgccgagcgc
     1321 tggatcgcgc gattgccgtc cgtggcgaca tgcttgtcga aggcatcgtg gacgcggtcg
     1381 aagcggaagc gcgcgagttg cgtcgccatc cgggcgaccg tcgccggatc gagcgggatc
     1441 gtgttcgggt agctgtacat gaacgtcacc cagttgggat cagcgacgac cttcacgata
     1501 tcccccgtga agagtgcacc cctcccgtcc ggcgtcccgc tccagtgcag caccatcccg
     1561 cccgggaaat gaccgccggt gcggatcacc gtgatgccgg gaatcggctt cagggtgtca
     1621 ccgctccagt actcgaccac gctgtccggg cgcatcaccc actggcgatt gctgtcgtgg
     1681 aggaagatcg gcacgtgccc cagtgcatga ctccactcga ccatcgtcgt gtagtagtgg
     1741 gcgtgggaga tcgcgatcgc cttgatcccc ccgcgcgcct cgatctcggc gatcgtcgcc
     1801 tcgtcgatca gcgcgacgag atcccagaga atgtttcctt ccggcgtctc gatcaggtag
     1861 gcattctg
//
LOCUS       DASTMD010000118         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2331903, whole genome shotgun sequence.
ACCESSION   DASTMD010000118 DASTMD010000000
VERSION     DASTMD010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331903"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFQ54_01880"
     CDS             complement(<1..390)
                     /locus_tag="VFQ54_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU0163761.1"
                     /translation="MLDYHVHTTRSIDCHTPILASCEAAIARGVREIAFTDHVDFEPA
                     DEGYGFYDYEGYLSDIERARDRFGDRLTILAAAEIDFNTRNTGQIEAFLGDHAYDFVI
                     GSVHYGENGEIIFSRYFDRRTLDDVFVP"
     gene            582..1361
                     /locus_tag="VFQ54_01885"
     CDS             582..1361
                     /locus_tag="VFQ54_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEU0163762.1"
                     /translation="MQDHLQRFLKDVGSPLGHVAEGIIQILILGAITFYLARYAGKFV
                     GRAMARRSFGRNGALLVGRLSSVAIWIAGISAIMATLGVSSTGILTLISASTVAISLS
                     LQDVLKNFVSGIFLLVERPFKVGDRIRVRDVVGEVQGIDIRTTLIRNTEGALVMVPNA
                     TIFTEILINRSHYRTRRLDITVTCGRNQLATINERIAAAVDGIEGVRRPLPAPTMRSA
                     TPEETIIELSLLIETSPDVEQNVIRRLIDALGDAKLEVTKP"
     gene            1361..1990
                     /gene="nadD"
                     /locus_tag="VFQ54_01890"
     CDS             1361..1990
                     /gene="nadD"
                     /locus_tag="VFQ54_01890"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU0163763.1"
                     /translation="MSANRIGIFGGTFDPIHIGHLAVAERACDELGVERVVFVPALRP
                     PHKPHRTISPIEDRLHMLELAIAGNSRFSWSDVDLHPDEPSYTVRMLERLGAEYPGAE
                     LYFIIGGDSLRDFDSWYQPDEILRLATLAVADRPGIQVDESIYHALPGLREKVVRFAA
                     PLLEVSSTALRERVGANKSIRYLVSEPVYRYIKERELYRRDSVLPDDGG"
BASE COUNT          374 a          638 c          631 g          387 t
ORIGIN      
        1 cggaacgaag acgtcgtcca gcgtgcggcg atcgaagtag cgcgagaaga tgatctcgcc
       61 gttctctcca tagtgaacgg agccgatcac gaaatcgtac gcgtggtctc cgaggaacgc
      121 ttcgatctgg ccggtgttgc gggtgttgaa gtcgatctcg gccgcggcga ggatcgtgag
      181 gcgatcgccg aagcggtcac gcgcgcgctc gatgtcggag agataaccct cgtagtcgta
      241 gaacccgtac ccttcgtcgg ccggttcgaa gtcgacgtga tcggtgaacg cgatctccct
      301 cacgccgcgc gcaatcgcgg cctcgcatga cgcgagaatc ggcgtgtgac aatcgataga
      361 tcgagttgtg tggacgtgat aatcgagcac agaagcctcc ccaccggcag cgagtgtacc
      421 tgagcgccga aggtctgtaa cagatgtacg tgcgcccaaa agtcacgttc cgggcgagac
      481 cgtcccccct cttcgggcgt cctcgtgcat ctgaccttcg atctcacgta ttctgtccct
      541 gcttggcaca cgagcgcgcg ggcgtcattc cggggagaaa gatgcaggac cacctccaac
      601 ggttcctcaa ggatgtggga tcgccgcttg ggcacgtcgc cgagggaatc atccagattc
      661 tgattctcgg cgcgatcacg ttctacctcg ctcgctatgc gggaaagttc gtcggacggg
      721 cgatggcccg gagatccttc ggtcgaaacg gggcgcttct cgtcggcagg ctctcgtccg
      781 tcgcgatctg gatcgccggg atcagcgcga tcatggcgac gctcggggtc agttcgacgg
      841 gaatcctgac gctgatcagc gcgagcacgg ttgcgatcag cctctcgctg caggacgttc
      901 tcaagaactt cgtctccggc atcttcctgc tcgtcgagcg cccgttcaag gtgggcgatc
      961 ggatccgggt gcgcgacgtc gtgggtgaag tccagggcat cgatatccgg accacgctga
     1021 tccggaatac cgagggcgcg ctggtgatgg ttccgaacgc gacgatcttc acggagattc
     1081 tcatcaaccg ctctcactac cggacgcgcc gcctggacat caccgtgacc tgcggtcgca
     1141 accagctggc gacgatcaac gagcggatcg cggcggcggt cgacggcatc gagggcgtgc
     1201 ggcgtccgct gcctgcccct acaatgcgat ccgcgacacc agaggagacg atcatcgagc
     1261 tgtcgctcct gatcgagaca tccccggacg tcgaacagaa tgtcattcgg cggctcatcg
     1321 acgcgttggg tgatgccaag ctggaggtca ccaagcccta gtgtcagcga atcgaatcgg
     1381 tatcttcggc ggcaccttcg atcccatcca catcgggcac ctcgcggtcg ccgaacgcgc
     1441 ctgcgacgaa ctcggcgtcg agcgggtcgt cttcgtcccg gccctgcgac cgccgcacaa
     1501 gccccatcgg acgatcagcc cgatcgagga tcgccttcat atgctggagc tcgcgatcgc
     1561 gggcaattca cgattcagct ggagcgatgt cgatctgcat ccggacgaac cgtcgtatac
     1621 tgtgaggatg ctggagcgtc tcggggccga gtatcccggc gccgaactct acttcatcat
     1681 cggcggtgac tcgcttcgcg actttgacag ctggtatcag ccggacgaga ttctgaggct
     1741 ggccaccctg gccgtcgccg atcgcccggg catccaggtc gacgagagca tctaccacgc
     1801 cctgccagga ctccgggaga aggtcgtgcg cttcgccgcg ccgcttctcg aagtgtcatc
     1861 aaccgccctc cgggagcggg tcggcgcgaa caagtccatc cgctacctcg tttccgagcc
     1921 cgtctaccgg tacattaagg agcgggaact ctatcggagg gattccgtct tacctgacga
     1981 cggcggatga attcatccgt gacaagtttg accgggcttc gtcgcaatcc
//
LOCUS       DASTMD010000119         5903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1171450, whole genome shotgun sequence.
ACCESSION   DASTMD010000119 DASTMD010000000
VERSION     DASTMD010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171450"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2021
                     /locus_tag="VFQ54_01895"
     CDS             <1..2021
                     /locus_tag="VFQ54_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163764.1"
                     /translation="PITACRTLEVAGLPTPTPTETSVPTNTPSPTPVPSLTINSGTDT
                     LIHLNLGDWIIYQTENVPTLAFFSGGGCLPQNYLSEYTNPDSGALNTSGFAPNYFIDN
                     QTSIQGYSGSFNAAGQPITTCRTIAVAGLPTATPTQTNVPTDTPTQTATVTATPTETA
                     TAPPTQTATPTPSLTINGATDDVTHVDYFSGNVTLDWTNIQLLNLYATADCTGALVNN
                     YTTGSGSRSYSAMFWATTVGGYVFSVKGFTSPNPTALTDCRAVIMDNAPVTPTLTSVP
                     SPTATATAAPPTATPTSVPTNTSTLVPTDTPTPQPTDTATLVPTDTPTVIPTSTATPA
                     PTDTLVPTDTATTIPTDTPPLSPTSTPTLVPTDTATPIPTDTPTATPEPVATETPVPT
                     TTATAEPTQTATIAPPETAVPTQPPTTEPTAIATQTATVTKTAVPTSIATETLVPTNS
                     ASATAISASTMTPESTATEISTTAVPPTATVTQTASNTPTATAAEGMLQIQVDTTDGG
                     DIPAGTTWSLSGVTPQGSSSGAGRVFASQTNTTQSGTLASPLPGGSLLPNTNPVAFGI
                     YTLTIDAPGYQLFIESIDHLGSTGTETPITVRLVAIPAASPVITAVPTGTTSASITPV
                     PTRMVAVNELPNTGAGTPGTGSWSLLLLMIAGGLAIGGIVPGFRLRRR"
     gene            complement(2036..3307)
                     /locus_tag="VFQ54_01900"
     CDS             complement(2036..3307)
                     /locus_tag="VFQ54_01900"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0163765.1"
                     /translation="MIAALPVRRDRKPLLALIISMTISLIGSQFTLIALPWFVLTTTG
                     SAAKAGLVGFAELLPALAVGIFGGVYVDRIGFKRVSVIADVVSGLGIVAIPLLYDTIG
                     LAFWQLLVLVFIGSLLNVPSVTAHRSMVPELAALAGIPLARVNGFVESLRSLATLLGT
                     PLAGFCVAWLGARQVLWLDAASFAFSAALVALLVPGAYFLAKPAIAANGYVKEVFAGL
                     KFIRKDQLLWPMVIVLALMNALTGSIGGLVLPVYAREEFGSAASLGVMLAATGAGAFL
                     GSTLYGVFAERLSRTLVWVVAFLLMPFELWIFTLSPSVPVLVVVFLAGGIISGPLNPL
                     MVTIRMERSPEALRGRVFSTYSAVAGAAQPLGMLVIGNMIQGAGFTPAVVATAICAQT
                     LGIVVILIPAFRRMDALREDRPVTAPAASRA"
     gene            complement(3304..3987)
                     /locus_tag="VFQ54_01905"
     CDS             complement(3304..3987)
                     /locus_tag="VFQ54_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0163766.1"
                     /translation="MAGEVEQERNTDDQLRESNVRVIDDPETLRLIADPLRLRLLELL
                     RAQPRTVTELADLLDVQRTKLYYHVKLLEAHDLIAVDRTRVVSGITEKRYRVTAYQLS
                     VDKALLGAPDSGQEPLDLLLSFILGEAAADIRRAVRAGLIDLDRTQEDAIGPRRLVIG
                     RSWYRLTPADVEVFEAAYEEFRAKIADLQGGFDPRPEADPGGGPDALLYEWLVAFYPI
                     VSPDDEAEQ"
     gene            complement(4312..>5903)
                     /locus_tag="VFQ54_01910"
     CDS             complement(4312..>5903)
                     /locus_tag="VFQ54_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163767.1"
                     /translation="SGQALDVESPTSGFTFQLPTLTANLAVTLNTSDNGDIPAGSTWS
                     LADPSGAAIDGGTLADAIPSGGTLPISVNPLIDGTYALTIDAGSGYEPYTGSIPVTSD
                     PTDFTAQIVTAGQTSTESTVQYTIQTDDGTDIPAGSTWQLTQADSNYTPVASGAVPPH
                     STSPFTVSIPNVPYGTYVLFFVSPDYIYYDAQTVAVDQPIFAYTTTLVHQQGDLAATI
                     ATSDGASVPDGTTWSLTDADGTVVQSGTVGDVDALPITNPIDYGVYVLQVTAPGYLGF
                     AAEIAIAQPSSSISIVLQKPAPTTGTVNLTVQTADGGAIPTNTIITIGGVSYVVPAGA
                     DQASVSAADVASSAVIPFDDVPAGTQPVSVTNADPYLDYSGTTDVVAGSAIDTTIVLQ
                     LAAVPSVTPEPTATTVPGASPTPTTVPGTTPTIAPGSTPTSTTTPLETPTTAPTTVAT
                     GTVPASTVTPTVGAATPIATVTVVPSKPGGGNVAVTTLPNTGQGAGTSDSRWELFALL
                     AAMVLIVGIGFTTTRLRSSRR"
BASE COUNT         1157 a         1930 c         1919 g          897 t
ORIGIN      
        1 agccgattac ggcctgccgc acgcttgaag ttgcaggcct cccaacaccc acgccgacgg
       61 aaacgagtgt cccgacaaat acgccgtccc caacgccggt gccgagcctg acgatcaaca
      121 gtgggacgga cacgttgatc cacctgaacc tcggcgactg gatcatctat caaaccgaga
      181 atgtgccgac gctcgccttc ttcagcggcg gcggttgcct tccccagaac tatctcagcg
      241 agtacacgaa ccccgacagc ggtgccctca atacgagtgg cttcgcgccg aattacttca
      301 tcgacaatca gaccagcatt cagggctatt ccgggagctt caacgccgcc gggcagccga
      361 tcaccacctg ccgcaccatt gcggtcgctg gcctcccgac ggccacaccg acgcaaacga
      421 acgtcccaac agatacgccg acacagaccg ccaccgtaac ggccacgccg acggagaccg
      481 caaccgcccc cccgacacag accgctacgc cgacgccgag cctgacgatc aacggcgcca
      541 cagacgacgt gacgcatgtc gactatttca gtggcaacgt gacgctggac tggaccaaca
      601 tccaactgct gaatctctat gccaccgcgg actgtacggg cgcgctcgtc aacaactaca
      661 ccaccgggag cggctcgcgt tcctattccg cgatgttctg ggcaacgacg gtgggtgggt
      721 atgtcttcag cgtgaagggc ttcacttcgc ccaaccccac ggcgctgact gattgccgtg
      781 ccgtgatcat ggacaatgcc ccggtcaccc caacgctgac atcggtccct tcgccgaccg
      841 ccaccgccac cgctgctcca ccaaccgcga cgccgacatc agtgcccacg aacacgtcga
      901 cgctggttcc aaccgacaca ccaacgccgc agcccaccga taccgcgacg ctggttccaa
      961 cggacacacc gaccgtgatc ccaacgagca ccgcaacgcc ggctccgacg gataccctgg
     1021 ttccgaccga cacagcaacc acgatcccca cggatacgcc gccattgtcc ccgaccagca
     1081 cgccgacgtt ggtgccaacg gatacagcga cgccgatccc aaccgacacc ccgaccgcca
     1141 cgccggagcc ggttgcaaca gaaacacctg tcccgacaac tacggcaacg gcagaaccga
     1201 cccaaaccgc aacgattgcc ccaccggaga cggctgttcc gacgcagccc cccactacgg
     1261 agccaaccgc gatcgcaacg cagaccgcga cggttacgaa aacggccgtt ccgacgagca
     1321 tcgcgacgga aacactcgtg ccgacgaatt cggcgagtgc cacggctatc agtgcgtcga
     1381 ccatgacacc ggagtccacc gcgacagaga tctcgaccac agcggttccg ccaaccgcga
     1441 ccgtcacgca aacggcctcg aacactccaa ctgccacggc tgcggaagga atgttgcaga
     1501 tccaggtgga cacgaccgat ggcggcgaca tccctgccgg cacgacctgg tcgctgagtg
     1561 gggtcacgcc tcagggatcg tccagtgggg ctggacgcgt cttcgcgtcc caaaccaaca
     1621 cgacccagag cgggactctg gcatctccgt tgcccggcgg gagtctgctg ccgaacacga
     1681 atccggttgc gttcgggatc tacacgctga cgatcgatgc acccggatac cagctgttca
     1741 ttgaatcgat cgaccatctc ggatcgactg gcacggagac gcccatcacg gttcgcctgg
     1801 tcgcgattcc cgcggcttca cccgtgatta cggcagtgcc caccgggacg acgagcgcat
     1861 cgatcactcc cgtaccgacg aggatggtgg ccgtcaacga actgccgaac accggagcgg
     1921 ggacgcccgg gaccggatcg tggtcgctgc tcttgctgat gattgcgggt ggtcttgcaa
     1981 tcggcgggat cgtgcctgga ttccggttgc gtcggcgata ggccgggaag acccgttacg
     2041 ccctgctggc cgccggcgcc gtcaccggtc gatcctcccg cagtgcatcc atgcggcgga
     2101 atgccgggat caggatcacg acgatcccga gtgtctgcgc gcagatcgcg gtcgcaacca
     2161 ccgccggggt gaagccggca ccctggatca tgtttccgat gacgagcatt cccagcggct
     2221 gcgcggcccc ggcgacggcc gagtaggtac tgaagacccg tccgcgcaac gcctccggac
     2281 ttcgctccat gcggatcgtc accatcaagg ggttgagcgg accactgatg atgccgccgg
     2341 cgaggaacac gacgacgagg acggggaccg acggggagag cgtgaagatc cagagttcga
     2401 acggcatcag caagaaggcg acgacccaga cgagggtacg ggagagccgc tccgcgaaga
     2461 cgccgtagag ggtcgaaccg agaaaggcgc ccgcgcccgt cgccgccagc atcacgccga
     2521 gtgatgccgc gctcccgaac tcctcacggg catagaccgg gaggacgagg ccgccgatcg
     2581 agccggtgag ggcgttcatc agcgccagca cgatcaccat cggccagagc aactggtcct
     2641 tgcggatgaa cttcagcccg gcgaagacct ctttcacata cccgttcgcg gcaatcgccg
     2701 gcttcgccag gaaataggct cccggtacga ggagcgcgac gagcgccgcc gagaacgcga
     2761 acgaggcggc atcgagccac aggacctgcc gtgcgccaag ccaggcgacg cagaatcccg
     2821 ccaatggcgt cccgagcagc gtcgcgaggc tgcgcaacga ttcgacgaag ccgttgacgc
     2881 gcgcgagtgg aattccggca agcgcggcaa gttccggcac catcgaacgg tgcgcggtca
     2941 ccgaggggac gttcagcagg gagccgatga agacgagcac gagcagttgc cagaacgcga
     3001 gcccgatcgt gtcgtagagg agcgggatcg ccacgatgcc gagaccactg acgacgtccg
     3061 cgatcacgct gacgcgcttg aagccaatgc gatcgacgta gacgccgccg aagatcccca
     3121 ccgcgagcgc cggcaggagt tcggcaaatc cgacgagacc ggctttcgcc gcgctgccgg
     3181 tcgtggtgag gacgaaccag ggcaacgcga tcagcgtgaa ctggctgccg atcaacgaga
     3241 tcgtcattga gatgatgagg gcgaggaggg gtttgcggtc gcgacggacg gggagcgcgg
     3301 cgatcattgc tctgcctcgt cgtccggcga aacgatcgga tagaaggcga ccaaccactc
     3361 gtagagcagg gcgtcgggac cacccccagg gtcggcttcg gggcgagggt cgaatccccc
     3421 ctggagatcg gcgatctttg cgcgaaattc ctcgtaggcc gcctcgaaga cctcgacatc
     3481 ggccggagtg agccgatacc aggagcgccc gatcaccagc cggcgcggcc cgatggcgtc
     3541 ctcctgcgtc cgatcgagat cgatcagacc ggcccggacg gcacgacgaa tatcggcggc
     3601 ggcctcgccg aggatgaatg agagcagcag gtcgagcggt tcctggccgg aatccggcgc
     3661 gccgagcaag gccttgtcga cgctcaattg gtaggccgtg acccgatacc gcttctcggt
     3721 gatcccggaa acgacccggg tgcggtcaac ggcgatcagg tcgtgcgcct caaggagctt
     3781 gacgtggtag tagagcttcg tgcgttggac atcgagaaga tcggcaagct ccgtgacggt
     3841 acggggctgc gcgcggagga gttcgaggag gcggaggcga agcgggtcgg cgatgaggcg
     3901 cagggtctcg gggtcgtcga tgacgcggac attgctctct cgaagctgat cgtctgtgtt
     3961 gcgttcctgc tcgacctcac cggccaccgg tcacctgcca ttcacagtca ttagaccatt
     4021 caactctatg tgaatgatac ggggcgggga tggtgttcga caagcgtgcc ggtggactcc
     4081 aacgggggct ggcattcgcg gcgcgcccac ggaacgtctc gccgtgccag atcacatcgg
     4141 accggaacgg cgacaagcgc cgtggggacg gaagccgcgg ggacggaact cgagcgtgtc
     4201 acgcatgcct gtcttcaggc agacagggag gtccaggagg cgggaagggc aatgagaagg
     4261 gcgggccagg tggcccgccc ttctcgttta cgtgatatcg acagggaacg actaccggcg
     4321 cgatgaacgc aggcgggtgg tcgtgaagcc gatgccaacg atgagcacca tcgccgcaag
     4381 gagggcgaag agttcccagc gtgagtcact cgttcccgcg ccctgaccgg tgttcggcaa
     4441 cgtggtcacc gccacgttgc caccacccgg cttcgacggg acgacggtaa ccgtcgcgat
     4501 cggggtcgcg gcaccaaccg tcggcgtgac tgtcgacgcg ggaacggtcc ccgttgccac
     4561 cgtcgtgggg gcggtcgtcg gcgtctccag cggtgtcgtt gtcgatgtcg gggtggaacc
     4621 cggcgcgatc gtcggggtgg taccgggcac cgtcgtcggc gtcggcgagg cacccggaac
     4681 ggtcgtcgcg gtcggctccg gagtcaccga cggaacggcg gcgagctgaa gaacgatcgt
     4741 ggtgtcgatc gccgatccgg cgacgacgtc tgtcgtcccg ctgtaatcga ggtacggatc
     4801 cgcgttcgtg accgaaaccg gttgcgtgcc ggccgggaca tcgtcgaacg gaatgaccgc
     4861 actactggcg acatccgccg cgctcaccga ggcctggtcc gcaccggcgg gaacgacgta
     4921 gctcacgccg ccgatggtga tgatcgtatt ggtcgggatc gccccgccgt cagccgtctg
     4981 cacggtcaga ttgacggtac ccgtcgtcgg cgccggtttc tggagcacga tcgagatgct
     5041 gctcgatggc tgggcgatcg cgatttcggc cgcgaagcca aggtagcctg gcgctgtcac
     5101 ctgcagcacg tagactccat agtcgatcgg gttcgtgatc gggagcgcat cgacgtcacc
     5161 gacggttccg ctctggacaa ccgtgccatc ggcatcggtg agcgaccagg tcgtgccatc
     5221 cgggacgctc gcgccatccg atgtcgcgat cgtggccgcg agatccccct gctgatgcac
     5281 gagggtcgtg gtgtaggcga agatcggctg atcgaccgcg accgtctgcg catcgtagta
     5341 gatgtaatcc ggcgagacga agaacagcac gtaggtcccg tagggcacat tcgggatcga
     5401 caccgtgaag ggcgaggtcg aatgcggcgg cacggcgcca gacgcgaccg gcgtgtagtt
     5461 gctgtcggcc tgggtcagct gccaggtgct gccggccgga atatccgtgc cgtcatcggt
     5521 ctggatggtg tactggaccg tcgattcggt cgatgtctgc ccggccgtca cgatctgggc
     5581 cgtgaagtcg gtcgggtcgc tcgtgacggg aatcgaaccg gtgtagggct cgtatccact
     5641 gccggcatcg atcgtcagcg cataggtgcc gtcgatcaat ggattgacgg agatcggcag
     5701 cgtgccgccg ctgggaatcg catcggccag cgtgccgccg tcgatcgccg cgccggaagg
     5761 atcggcaagt gaccaggtcg atcccgccgg gatgtcgcca ttgtcgctgg tattcagggt
     5821 cacggcgaga ttcgccgtca gggtcggcag ctggaaggta aagccgctcg tcggcgactc
     5881 gacgtcgagc gcctggccgg aac
//
LOCUS       DASTMD010000120         1677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_458167, whole genome shotgun sequence.
ACCESSION   DASTMD010000120 DASTMD010000000
VERSION     DASTMD010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458167"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VFQ54_01915"
     CDS             <1..518
                     /locus_tag="VFQ54_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MSMEG_1061 family FMN-dependent PPOX-type
                     flavoprotein"
                     /protein_id="HEU0163768.1"
                     /translation="IRDYIHASPFFLLGTSDALGNCDVTPRGDTTSVIRILDDQTLVI
                     PDRPGNKRADSFRNILGNPHVGLLFVIPGVEEALRINGRATITKDPEILAEMSIQGKQ
                     PVLGIAVEIDEVFVHCARAMLRARLWKPETWPDDDAIPTLAAIWNEQKNLPPPDESAG
                     KRREEYRERLY"
     gene            complement(512..913)
                     /locus_tag="VFQ54_01920"
     CDS             complement(512..913)
                     /locus_tag="VFQ54_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU0163769.1"
                     /translation="MTIGEIAARAGMNTSALRFYERVGLLPVARRVNGQRRYDPDVLA
                     QLALIRMAQEANFTIEEIRTLTTGFPEGTPPSERWQELAQRKLPEVAAMIDRLETVRL
                     VLEESLRCDCLTLDACARLGWNAQASMPANQ"
     gene            1014..1316
                     /locus_tag="VFQ54_01925"
     CDS             1014..1316
                     /locus_tag="VFQ54_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163770.1"
                     /translation="MEERVIACSLADVELARRSDLVRRDLFAGALVRAELAAGYAFRF
                     PGDGDWDARIQEFVGIERRCCSFFQFEIVFEAGMGSIWLRLTGPEGTKQFIEQAFE"
     gene            1360..>1677
                     /locus_tag="VFQ54_01930"
     CDS             1360..>1677
                     /locus_tag="VFQ54_01930"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0163771.1"
                     /translation="MNERRSRPLPVIQPDACVSNPVDPPPMAEETRVTLLATFKALSD
                     ATRFEVFRFIAGQDAPICACDIVDRFDVSQPTIAHHLKVLRQAGLITATKSGVWVYYA
                     LDPR"
BASE COUNT          287 a          511 c          555 g          324 t
ORIGIN      
        1 tgatccggga ctacattcac gcctcgccgt tcttcctgct tggaacctcg gatgccctcg
       61 gcaactgcga cgtgacgccg cgcggcgata cgacgagcgt gatccggatt ctcgacgatc
      121 agacgctggt gatcccggat cgcccgggga acaagcgggc cgattcgttc cggaacatcc
      181 tcgggaaccc gcacgtcggg ctgctcttcg tgatcccggg tgtcgaggag gcgctgcgca
      241 tcaacggtcg ggcgacgatc acgaaggatc cggagatcct ggccgagatg tcgatccagg
      301 ggaagcagcc agtgctcggg atcgcggtcg agatcgatga ggtcttcgtc cactgcgcgc
      361 gggcgatgct ccgggcccgg ctctggaagc cggagacgtg gccggatgat gacgcgatcc
      421 caacgctcgc cgcgatctgg aacgagcaga agaacctgcc gccgccggac gagtccgccg
      481 gcaagcgtcg cgaggaatac cgggagcgtc tttactgatt cgccggcatt gatgcctgtg
      541 cgttccagcc gaggcgcgcg caggcgtcca gcgtgaggca atcgcagcgc aggctctcct
      601 cgagcacgag gcggacggtt tccagccggt cgatcatcgc cgcgacttcc ggtaacttgc
      661 gctgcgcgag ttcctgccag cgttcggaag gcggggtccc ctccgggaag cccgtcgtca
      721 gcgtccggat ttcctcgatc gtgaaattgg cctcctgcgc catccggatc agcgccagtt
      781 gggcgagcac gtccggatca taccggcgct gcccgttgac ccggcgtgcc actggcagca
      841 gccccacgcg ttcgtaaaat cgaagcgccg aggtgttcat tcctgcccgt gctgcaatct
      901 caccgatcgt catggccgct ctcctcttga cttgaagttc acttcaagtc ctaggctaca
      961 cgggacgtca attcagggcg tcgccgctgt cggcccgtga ggagggattg gcgatggaag
     1021 agcgtgtgat cgcatgcagt ctggcggacg tcgaacttgc caggcgatcg gacctggtcc
     1081 ggcgcgatct ttttgccgga gcgctggtgc gagcggaact tgccgccggt tatgcattcc
     1141 gctttcccgg tgatggcgat tgggacgcga ggatccagga gttcgtgggg atcgagcggc
     1201 ggtgctgctc gttcttccag ttcgagatcg tcttcgaggc ggggatggga tcgatctggc
     1261 ttcgactgac cggtcccgag gggacgaagc agttcatcga gcaggccttc gaatgacaag
     1321 atattgacag acgtctatgt cttgcgctag catcgcggca tgaacgaacg acggtcccga
     1381 ccactcccgg tcattcaacc tgatgcatgt gtcagcaatc ccgtcgatcc gccgccgatg
     1441 gcggaagaga cgcgggtgac gttgctcgcg acgttcaagg cgctttcgga tgcgacccga
     1501 ttcgaggtct tccggttcat cgccgggcag gacgcaccga tctgtgcgtg cgatatcgtc
     1561 gatcggttcg atgtcagcca gccgacgatc gcccatcacc tgaaagtgtt gcgccaggcc
     1621 gggttgatca cggcgacgaa gagtggcgtc tgggtgtact acgcgctgga tccgcgc
//
LOCUS       DASTMD010000121         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_636683, whole genome shotgun sequence.
ACCESSION   DASTMD010000121 DASTMD010000000
VERSION     DASTMD010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636683"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFQ54_01935"
     CDS             <1..691
                     /locus_tag="VFQ54_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU0163772.1"
                     /translation="VPFIIARPRVRWTWVLAGIQLVGVALTFSRGSLIAIVAGCALAL
                     ALAGYRRQIVGLAGVVVVIAALLFVFARDRLLDPGGGGHEPTRFALWRSAWHMIRDRP
                     LFGVGPDQFLYAYWRRYVEPVGWPERYTSHPHNMILDVWLRLGVIGLASFATLIVGVV
                     SQAVQIWREKRDDWLVRGSVVALFGGIVHGMFDNSFFLADLATMTWLFVALIAVSARP
                     QQSPVVAGAPA"
     gene            688..>2086
                     /locus_tag="VFQ54_01940"
     CDS             688..>2086
                     /locus_tag="VFQ54_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU0163773.1"
                     /translation="MTVGLPIDPARGWAVLLAVGTFGVLVLLPFPESCFAVAVAAGIV
                     VAWRSPATMLGLLAASIPVQAEGSFHIAGLPFTWTRAALLCLVIGWLLARERQPVRID
                     PIAWGYIAILLFLGTSILASPAVGGWAGEIYRWLAATVVYIIARTILRSRSDVVTAIS
                     GIALGVIGICLVGFYQVITVTGPPSFVVDGILRAYGSFGEPNPFAAYLELSVLLLIGA
                     FAATQRGWADTSRHTRVLIGTGIVLGMAMLFLTQSRGGWIGFAAGLLTLGLVLDRRQR
                     WAAAGVGVVLACLVLVSPVGSKVTDRFASLTSSADVQVTSANWANQERRAHWGAAISM
                     MKANPWTGIGAGAFNDRYREYTPVWRFRIGRGHAHDGFLQLAAEAGIPGLLAFSSWFL
                     AMLVTLGRTLRRMRGDRSCAPLLVAGLATVVAFGVHSLVDYLNVLSLGIELSIVVAVV
                     LAAGEISFRDRRPTNP"
BASE COUNT          274 a          661 c          691 g          460 t
ORIGIN      
        1 cgtgccgttc atcatcgctc gcccgagggt gcgttggaca tgggtactgg cgggaatcca
       61 gctggtcggc gtcgccctga cgttctcgcg gggatcgctg atcgcaatcg ttgccggatg
      121 cgccctggcg ctggccctcg cgggctatcg cagacagatc gtcggattgg ccggtgtggt
      181 cgtcgtgatc gcggccctgc ttttcgtctt cgctcgcgat cgattgctcg acccgggtgg
      241 cggcggccac gagccgacgc gattcgcgtt gtggcgctcg gcgtggcaca tgattcgcga
      301 ccgtccgctc tttggcgtgg ggccggacca gtttctctat gcgtattggc gccgctacgt
      361 ggaacccgtg ggatggcccg agcggtacac ctcgcatccc cacaacatga tcctcgatgt
      421 ctggctccgg ctgggagtga tcgggctcgc gtcctttgcg acgctcatcg tcggcgtcgt
      481 cagccaggcc gtccagatct ggcgggagaa acgggatgac tggctggttc gcggcagtgt
      541 cgtcgccctc ttcggaggta tcgtccacgg tatgttcgac aacagcttct tcctggccga
      601 cctcgcgacg atgacctggc tcttcgttgc actgatcgcc gtcagcgcgc gaccgcagca
      661 gtcgccggtc gttgccgggg cccccgcatg accgtcggac tgccgatcga cccggcacgc
      721 ggctgggccg ttctcctcgc ggtcggcaca ttcggcgtcc tcgtgctcct tccgtttccc
      781 gagagttgct tcgccgtcgc ggttgccgcc ggcatcgtgg tggcctggcg atcgccggcc
      841 acgatgctgg gactgctcgc ggcgtcgatt ccggttcagg ccgagggaag cttccacatc
      901 gcgggacttc cattcacgtg gacccgcgct gctctcctgt gcctcgtcat tggttggctt
      961 ctggcgcgag aacgtcagcc ggtccgcatc gatccgatcg cctggggata tatcgcgatc
     1021 ctgctcttcc tcgggacgtc gatcctggcg tctcccgcgg tcggcggttg ggcaggcgag
     1081 atctatcgtt ggctggcggc gaccgtcgtc tacatcatcg cgcggacgat cctgcgctcc
     1141 cggtccgatg tcgtcaccgc gatctcgggg attgccctcg gcgtgatcgg gatctgcctc
     1201 gtcgggttct accaggtcat cacggtgacc ggaccgccat cattcgtcgt cgatggcatc
     1261 ctgcgggcct atggctcgtt cggggagccc aacccgttcg ccgcctatct cgaactcagc
     1321 gtgctgctgc tgatcggagc gttcgcggcg acgcaacgtg gttgggccga tacctcgcgt
     1381 catacgcgtg tcctgatcgg aaccgggatc gtgctcggca tggcgatgct ctttctcacg
     1441 cagtcgcgtg gcggctggat cggctttgcc gcggggctcc tcacgctcgg attggtcctc
     1501 gatcgacggc agcgttgggc cgccgccggg gtcggcgtcg ttcttgcgtg cctggtcctg
     1561 gtctctcccg tggggagcaa ggtgacggat cgctttgcgt cactcacgtc atccgccgat
     1621 gtgcaggtga cgtcggcgaa ctgggccaat caggaacgac gcgctcactg gggcgcggcg
     1681 atcagcatga tgaaagcaaa tccgtggact ggaatcgggg ccggggcgtt caatgaccgc
     1741 tatagggagt acactcctgt ttggcgattc aggatcggtc gcggccacgc acacgacggt
     1801 ttccttcaac tggccgcgga agccggaatt cccgggttgc tcgcattctc ctcctggttt
     1861 ctggcgatgc ttgtgacact tgggagaacg ctgagacgta tgagaggtga cagatcgtgc
     1921 gcgccacttc tcgtcgccgg acttgcgact gtcgtggcgt ttggagtgca cagcctggtt
     1981 gactacttga acgtcctgag tctcgggatc gaactgagta tcgtcgttgc ggtcgtgctg
     2041 gccgccggcg agatttcctt ccgggaccga cgtccaacca atccgc
//
LOCUS       DASTMD010000122         1710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1707368, whole genome shotgun sequence.
ACCESSION   DASTMD010000122 DASTMD010000000
VERSION     DASTMD010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707368"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFQ54_01945"
     CDS             <1..335
                     /locus_tag="VFQ54_01945"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HEU0163774.1"
                     /translation="EGYAAAQGPEATVVLDTTLTPELVQEGIARDFVRAVQDARKQLE
                     LNIEDTIALTYAAPVEISAAVTAFGDYIAREVLATEILAADDVTGGEGYTQVKVGTET
                     VDIAISKR"
     gene            452..1564
                     /gene="ychF"
                     /locus_tag="VFQ54_01950"
     CDS             452..1564
                     /gene="ychF"
                     /locus_tag="VFQ54_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU0163775.1"
                     /translation="MATKLVIIGLPSTGKTTVFNALTHADAATGTFGSSGDEPNLATV
                     KVPDPRLDALTALFKPESKVPADVQYLDVAGIAKGIAEKGMGGQLLGHLSQADALVHV
                     ARAFEDPNVPHPEETVDALRDIETLDLELLFSDLALIEKRILRIESQLGKMRGPEKEI
                     NERELAVLVTFKAALENDQPLREIVDDIDPDEYKLVRGFGFLTAKPLLILLNTGENQL
                     GEPTEKLLAEARAKFSGKKRSIDAIAGKIEMEIGQLDPEDAEVFMSDLGIAESGLDRI
                     IRESFNLLGLMPFFTVGPEECRAWTIRRGATAVEAAGEIHSDIQRGFIRAVVIGYEAM
                     IKAGSMAAVRAAGELRREGKTYVVEDGDIIEFLFNV"
BASE COUNT          336 a          529 c          544 g          301 t
ORIGIN      
        1 gggaaggcta cgccgcagcc cagggcccgg aggcgaccgt cgtgctcgac acgacattga
       61 cgcctgagct cgtccaggaa ggtatcgctc gcgacttcgt ccgcgcggta caggatgcgc
      121 gcaagcagct cgagttgaac atcgaggaca cgatcgccct cacctatgcg gctccagtcg
      181 agatctccgc ggcggtgacc gccttcggcg actacatcgc gcgggaggtt ctggccaccg
      241 agatcctggc ggctgacgat gtcactggcg gcgaagggta cactcaggtg aaagtcggca
      301 ccgaaaccgt cgacatcgcc atctcgaaac gctgacccgc cgatgacgcg aaggtcatca
      361 tccaaaccga cgtgacgcga ggcgttcgca aggcgggact ccaatgtccc gccttcgcat
      421 gagcgcggag caaggagaag tcgaaggcaa cgtggctacc aaacttgtga tcatcggtct
      481 cccctcgacg ggaaagacca ccgtcttcaa cgcgctgact cacgccgacg cggcgaccgg
      541 gacgttcggg tcgagtggcg acgagcccaa cctggcgact gtgaaggtgc cggatcctcg
      601 cctcgatgcg ctcaccgcgc tgttcaagcc cgagagcaag gtgccggctg atgtccagta
      661 cctcgatgtc gccggcatcg cgaagggaat cgccgagaag gggatgggtg gccagttgct
      721 cggtcatctc tcgcaggccg atgcactcgt ccacgtagcc cgtgccttcg aggatcccaa
      781 cgtgccgcat ccggaggaga cggtcgatgc cctccgcgat atcgagacgc tcgatctcga
      841 actgctgttc agtgatctcg cgctgatcga gaaacggatc ctgcgcatcg agagccagct
      901 cggcaagatg cgcggcccgg agaaggagat caacgagcgc gagctcgcgg tgctggtcac
      961 cttcaaggcg gcgctcgaga atgaccagcc gctccgcgag atcgtcgacg acatcgatcc
     1021 cgacgaatac aagctcgtgc gtggatttgg cttcctgacc gcgaagccgc tgctgatcct
     1081 cctcaacacc ggggagaacc agctcggcga gccaaccgag aagctgctgg ccgaagcccg
     1141 cgcgaagttt tccggcaaga agcggtcgat cgatgcgatc gccggcaaga tcgagatgga
     1201 gatcggtcag ctcgatcctg aggatgccga ggtcttcatg tcggatctcg gtatcgcgga
     1261 gtcgggtctc gaccgcatca tccgcgagtc attcaacctc ctcggcctga tgccgttctt
     1321 cacggtcggt ccggaagagt gccgggcctg gacgatccgc cgcggcgcga cggctgtcga
     1381 ggcagcaggt gaaatccact ccgacatcca gcgcggtttc attcgcgccg tcgtcatcgg
     1441 ctacgaggcg atgatcaagg ccggctcgat ggccgccgtc cgcgccgccg gcgaactccg
     1501 tcgcgagggc aagacctatg tggttgagga tggggatatc atcgaattcc tcttcaacgt
     1561 ctagcaaatt cacgaggctg ttcgatcgga acgagagggg atggccgttg ggccgtcccc
     1621 tctcgttcgt taccatcgat gcgttcttgt gtttagcatg gcgtagaacc ttccccggac
     1681 aagatgccgg atgatgaaat ccttggtggg
//
LOCUS       DASTMD010000123         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2243376, whole genome shotgun sequence.
ACCESSION   DASTMD010000123 DASTMD010000000
VERSION     DASTMD010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2243376"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VFQ54_01955"
     CDS             <1..757
                     /locus_tag="VFQ54_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163776.1"
                     /translation="CVKQGDDHVLPLVTTDYLARLGALPSTRTRSTRAGNASDLLPLA
                     TSALRDPTGIHLWPAEMLPDGRVEVSATADGASRQAAFSSTYYYVFLKNNDRWYLDEA
                     TVVSRGGYTEPSRALPDSREEAQNVPNPLGVHLQLDVDPEGILSGDAGVMASLISQLQ
                     LALAPYAADDAYCQLTSITIATTAPNGEQVSAKIWELFQQKESELITEPGVLNSIFPM
                     VYLVAGSTAAVPGTVTIDLYFPVTCRPPVLATP"
     gene            897..2795
                     /locus_tag="VFQ54_01960"
     CDS             897..2795
                     /locus_tag="VFQ54_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0163777.1"
                     /translation="MESNQSRGPLARLYASLSKGHISRRQFIERTGALGVGASLAYTL
                     ANAGSAMAAGDTNPFRNGFISLAQDATPQAIPSNGTDSQTRGDGGELKILQWQAPSEL
                     SPHVATGTKDFMAAALVLEPLMQYLEDGSLVANIITELPSVDNGLLAADLTSVTYKLI
                     PNITWSDGEPLTADDIQYTWQWVMNKDNASVHYGVYAPIKNIEVIDPLTAKLTFNAPN
                     PYWAESFAGTDSGYIYPKHILEPGGQKANDAFRLKPIGTGPYIVDSFSPNDQAVFVIN
                     DKFRFPTKPFFSKVTIKGGGDAASAARAVLQTADYDFAWNLAIEPELLNSMATADSPG
                     VVLSSPGSSVEQIQLQFADPDKTVDGQKAEMNTPHPFFTDKTVRQAFALAIDRDTIAN
                     KFYLGGDKEPAVANVVSGIPAIESPNNAVEFDIDKANQMLDAAGWTKDGNTRKKDGVE
                     LKLSYATTIASVRQKTQAVIKQDLEKIGFSVQLVQVDAGIYFDSSPSNDQNRGHFYWD
                     LEEYANQALSPRPLSYVNSWYSGKDRSNIAQKSNQWSGGNANRYVNPDFDKLYEEAVS
                     ETDAEKLAQLYIQMNDVVVQDVAVVALVRTSTKCAASKRLNPANFAFGPFTYQYWNIA
                     NWNLAPGQ"
     gene            complement(3077..>3359)
                     /locus_tag="VFQ54_01965"
     CDS             complement(3077..>3359)
                     /locus_tag="VFQ54_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0163778.1"
                     /translation="AQQSNQWSGQNYTRWQDADYDAAWEQAQTETDAETLAQLFIKMN
                     DLVITNNVIVPLVRVATINGFSRKLNLDNVALAAFSYDTWNIANWRTNS"
BASE COUNT          644 a         1100 c          999 g          616 t
ORIGIN      
        1 ctgcgtgaag cagggcgacg accacgtgct gccgctagtg acgacggact atctcgcccg
       61 actcggtgca ttaccgtcca cccggacgcg atccactagg gccgggaatg cgagcgacct
      121 cttgccactt gccacgagcg cgctcaggga cccgaccggt atacacctct ggccggcaga
      181 gatgctgcct gacgggcgtg tcgaggtaag cgcgacggcg gacggcgcat cacgccaggc
      241 cgcgtttagt tccacgtact attatgtctt tctgaaaaat aatgaccgct ggtatctcga
      301 cgaggcgacg gttgtttctc gcggggggta tacggagccg tcgcgggcat tgcccgactc
      361 gcgagaagaa gcacagaatg tcccgaatcc cctcggcgtc catctgcaac tggacgtcga
      421 tcccgaaggt atcctcagtg gcgatgccgg ggtcatggca tcgttgattt cacaactcca
      481 gcttgccctg gcaccctatg ctgcggacga cgcctactgc cagctgacga gtatcacgat
      541 cgcgacgacc gcccccaacg gcgagcaggt ctcggcgaag atatgggagc tgttccagca
      601 gaaggagtcg gaactgatca ctgaacccgg ggttctgaat agcatcttcc cgatggtcta
      661 tctggtcgcc ggttcgaccg ccgctgttcc aggaaccgtg acgatcgatc tctacttccc
      721 ggttacctgt cgtcccccgg tccttgccac accataggca tttcgcacaa ggaaattagc
      781 atgagcgcat tacccgcttg ccgggaatgc gcttttgtgt gcatatgtgg ctgatccatc
      841 tctctgggcg gcaggggtcc gctcacgttg tctcgctttc caggagttgt caattcatgg
      901 aatccaacca gtcgcgcggc ccgctcgcgc gcctctatgc atccctgtcc aaaggacaca
      961 tcagccgccg tcagttcatc gagcgaacgg gcgccctcgg cgtcggcgcc agcctcgcct
     1021 acacgctggc gaatgccggg tcggcgatgg ccgccggtga caccaacccg ttccgcaatg
     1081 gcttcatcag cctcgcccag gacgcgacgc cgcaggcgat tccatccaat ggcaccgaca
     1141 gccagacccg cggcgatggc ggcgagctca agatcctgca gtggcaggcg ccgagcgagc
     1201 tctcgccaca cgtcgccacc ggcaccaagg acttcatggc cgccgcactc gtgctcgaac
     1261 cgctcatgca gtacctcgag gatggatcgc tcgtcgcgaa catcatcacc gagctgccga
     1321 gtgtcgacaa cggtctcctt gccgccgatc tcaccagcgt cacctacaag ctgatcccga
     1381 acatcacctg gagcgatggc gagccgctga cggccgacga catccagtac acgtggcaat
     1441 gggtgatgaa caaggacaac gcctcggtgc actacggcgt ctacgcgccg atcaagaaca
     1501 tcgaggtcat cgatccgctg acggcgaaac tgaccttcaa tgcgccgaat ccctactggg
     1561 cggagtcctt cgccggcacc gacagcggct atatctatcc gaagcacatc ctcgagccgg
     1621 gcggccagaa ggcgaacgat gccttccgcc tgaagccgat cggcaccggt ccgtacatcg
     1681 tcgattcgtt ctcgccgaac gatcaggcgg tcttcgtgat caacgacaag ttccgcttcc
     1741 cgaccaagcc gttcttcagc aaggtcacga tcaagggtgg cggcgacgcg gcctcggccg
     1801 cccgcgcggt cctccagacc gccgactacg acttcgcgtg gaacctcgcg atcgagccgg
     1861 agctcctgaa cagcatggcg acggcggaca gcccgggcgt cgtgctctcg agccccgggt
     1921 cgtcggtcga gcagatccag cttcagttcg ccgaccccga caagacggtc gatggccaga
     1981 aggcggaaat gaacacgccg cacccgttct tcaccgacaa gacggtgcgc caggccttcg
     2041 ccctggcgat cgatcgcgac accatcgcca acaagttcta tctcggtggc gacaaggaac
     2101 ctgcggtcgc gaatgtcgtc tccggtatcc cggcgatcga atcaccgaac aacgccgtcg
     2161 agttcgacat cgacaaggcg aaccagatgc tggacgccgc gggctggacc aaggatggca
     2221 acacccgcaa gaaggatggc gtcgagctga agctctccta tgcgaccacg atcgcctcgg
     2281 tccgccagaa gacgcaggcc gtgatcaagc aggacctcga gaagatcggc ttctccgtcc
     2341 agctcgtcca ggtcgacgcc ggcatctatt tcgacagttc gccgtcgaac gaccagaacc
     2401 gcggtcactt ctactgggac ctcgaggaat acgccaacca ggcgctttcc ccgcgtccgc
     2461 tctcctatgt caacagctgg tattccggca aggaccgctc caacatcgcg cagaagtcga
     2521 accagtggag cggcggcaat gccaatcgct atgtcaatcc ggacttcgac aagctctacg
     2581 aggaagccgt cagtgagacg gatgccgaga agctcgcgca gctctacatc cagatgaacg
     2641 acgtggtcgt gcaggatgtc gcggtggtcg ccctcgtgcg cacgagcacg aagtgcgcgg
     2701 cgtcgaagcg cctgaacccg gcgaacttcg cgttcggacc gttcacctat cagtactgga
     2761 acatcgcgaa ctggaatctc gcgccggggc aatagacccg gtccgacaac ctgcgtcgcg
     2821 ttgcatgagg ggagagccgg agattgctct cccctcatgc catgtcgccc gcgggcccga
     2881 cgtctcttcg aattgcccgg gtttgtgggg caagaccgtg tgtcttgccc ttgcttgccg
     2941 cggcgccggt ttctctgaat cgtgcggtgt cccagatgat cgtcgcgcaa gaagggggtg
     3001 gcggatatcc cgccaccccc ttcttctcgt tctgcgcgtg ccgcgactgc atccggatcc
     3061 ggatgttgcc ggccggttac gagttggtgc gccagttggc gatgttccac gtgtcgtagg
     3121 agaacgccgc gagggcgaca ttgtccaggt tcagcttgcg cgagaagccg ttgatcgtcg
     3181 cgacgcgcac gagcggcacg atcacgttgt tcgtgatcac caggtcgttc atcttgatga
     3241 agagctgggc cagcgtctcg gcatcggtct cggtctgcgc ctgttcccac gccgcgtcat
     3301 agtccgcgtc ctgccagcgg gtgtagttct gaccggacca ctggttcgac tgctgggcg
//
LOCUS       DASTMD010000124         1927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_548028, whole genome shotgun sequence.
ACCESSION   DASTMD010000124 DASTMD010000000
VERSION     DASTMD010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548028"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFQ54_01970"
     CDS             <1..427
                     /locus_tag="VFQ54_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0163779.1"
                     /translation="STRGDQPTTSFSGLGIHVVGAGEFMTLTAVGGVSIERDREAEFI
                     AIATFAEIRARIDAWLERLHPLLAKLTEAELAARNTHERYQDRVWTNAQVLLHSLDHS
                     ALHLGHAQIQRQLWNDERAGKRTDGVQRITDEAAGHTTA"
     gene            complement(744..965)
                     /locus_tag="VFQ54_01975"
     CDS             complement(744..965)
                     /locus_tag="VFQ54_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163780.1"
                     /translation="MEVEGRLEGIQRFTIHGGPYGRLFFSLPEDPETVHQCQLPEEAF
                     DRDLAPGDAIVITMLIRTVMEVRKAPESS"
     gene            complement(1034..1780)
                     /locus_tag="VFQ54_01980"
     CDS             complement(1034..1780)
                     /locus_tag="VFQ54_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163781.1"
                     /translation="MTTEPPHSPGDEPVAPESLSLSNAPEPASAPVAPYLPDASLAED
                     VPPDVRGRRPLVFCGMCGALNPATSFYCAACGTTLVDAFHATEGLRVFERPDSASRLI
                     EIVPSGKELEVVEDANAPEDFVRVRLEYGRLGYIRLQDVATMAQAAGTGAGTPSAADL
                     AINTDARGCVGTTGALAAIGLVVILGALMIAIIARADIYDQGTLGIVFCFGLGPLLAL
                     TVGLYLYARGREERFMAAEDERQEHDRSGQ"
BASE COUNT          351 a          665 c          605 g          306 t
ORIGIN      
        1 gtcgacacgt ggcgaccagc cgacgacgag cttctccggc ctcggcatcc atgtcgttgg
       61 cgccggcgag ttcatgacgt tgacggccgt cggcggcgtg tcgatcgaac gcgaccggga
      121 agcggagttc atcgcgatcg cgaccttcgc cgagatccgc gcccgcatcg acgcctggct
      181 ggagcgactc cacccgttgc tcgcgaagct gacggaggcg gaacttgccg cccgcaatac
      241 gcacgagcgc taccaggatc gggtctggac gaacgcccag gtgctccttc attcgctgga
      301 ccattcggcg ctgcatctcg gccacgccca gatccagcgg cagctctgga acgacgaacg
      361 cgccggcaag cggaccgatg gcgtgcagcg gatcaccgac gaagcggccg gccacaccac
      421 cgcctgagtc gccggcggcg atcttctgtc tcccgccaca tcccgtaggg gcgaacctgg
      481 tgttcgccct ggtcgtccga aacgagcgcc ctgcaccgcg gcctgctagc cgcccacgtc
      541 atccgttacg gtggaggtgc ggcgccgtct ggcccgcgac gaagcgcagt cgagggatcg
      601 catcccatgt acgatgcctc ccctgcccgc ctcttttgcc aacgggtcgt accgggagca
      661 cgcgacgacc agccgatccc tcgacaggct cgggaagacg tgctcgggaa gacgtacgtg
      721 tctggccccg gtttccgtga aggctacgac gattcggggg ccttccggac ctccatgacc
      781 gtgcggatga gcatcgtgat cacgatcgcg tcacccggcg caagatcacg atcgaacgcc
      841 tcctccggca actggcattg atggacggtc tccggatcct ccggcaggct gaagaagagc
      901 cgcccatatg gcccgccgtg aatcgtgaac cgctgaattc cctcgagtcg cccctcgact
      961 tccacacgca ctcctcatgt atccggctga ttcatctggc cagccaggag attcatctac
     1021 gatatcatcg taatcactgg ccggatcggt catgctcctg ccgctcgtct tccgccgcca
     1081 tgaaacgttc ctcgcgcccc cgcgcgtaga ggtagagccc caccgtcaac gccaggagcg
     1141 gaccgagtcc gaagcagaag acgatcccca gcgtcccctg atcgtagatg tccgcgcgcg
     1201 cgatgatcgc gatcatcagc gcgcccagga tcaccacgag gccgatcgcg gcaagagcgc
     1261 ccgtcgtgcc gacgcagccg cgcgcatccg tgttgatcgc caagtcggcc gccgacggtg
     1321 tcccggcccc ggtgcccgcc gcctgcgcca tcgtcgccac gtcctggaga cgaatgtacc
     1381 ccaggcgacc gtattccagc cggacgcgga cgaaatcctc gggcgcattg gcatcctcga
     1441 cgacctcgag ctccttgccg gagggcacga tctcgatcag gcgcgaagcg gaatccggac
     1501 gttcgaagac acgcaatcct tccgttgcat ggaaggcatc gaccagcgtc gtgccgcacg
     1561 ccgcgcagta gaagctcgtg gcaggattga gcgcgccaca catgccgcag aagacgagcg
     1621 gacggcgtcc ccggacatct ggcggaacgt cctctgcgag cgaagcgtcg ggcagatacg
     1681 gggcaacagg ggccgatgcg ggctccggcg cgttcgaaag cgagagactt tccggcgcga
     1741 ccggctcatc cccgggtgaa tgcggtggtt cggtagtcac gaggagcgcg acctccggat
     1801 tcgacgatcg atcgccatcg tgacgtgcag ccggcggacg agcgagcgat gcacccggca
     1861 accgatgatg gcgagaaagc gtttacgttc tgataacgta ccggactgta ctccatcacg
     1921 acgggtg
//
LOCUS       DASTMD010000125         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1975750, whole genome shotgun sequence.
ACCESSION   DASTMD010000125 DASTMD010000000
VERSION     DASTMD010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1975750"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1669
                     /gene="treY"
                     /locus_tag="VFQ54_01985"
     CDS             <1..1669
                     /gene="treY"
                     /locus_tag="VFQ54_01985"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEU0163782.1"
                     /translation="TVGYEFAASVSGLFVDAQAESLFNRIYGRFTGDDIRYGELVYEM
                     KLRQIREAFASELNVLVNMLGRLSEHDRHSRDFTNAEFREVLRETIACFAVYRTYVTF
                     DDVGAADRDRRAIQSAIAMAARKRGAIDRTVFSFLEQTLLAEGPVDDEVTRQRRAFTM
                     KLQQLTGPVMAKGVEDTAFYRFNRLVSLNEVGGDPSRFGASVEDFHRQNRARLRSWPD
                     GLLAGSTHDTKRGEDVRAWISVLSEIPTQWRAALNRWSRLNRKLKVKREGILLPHRVD
                     EYVIYQTLIGIWPPESMSNAERTALGTRLGDYMIKVAREASRATSWIDPDQGYEDALS
                     GFIQGLLERRRSRLFLEDFEGFMERVRPAGMLNALGQHLLRLTAPGVPDIYQGTELWD
                     DSLVDPDNRRPVDFSIRATNAQAFPQMASPAMTLANPAVKQFVTARTLAVRAAHPDLF
                     ARGAYLPLIVEGPGAEHVVAFARTATDACAIVIVPRLVLGFGGGASDAGIRAGNTLTL
                     PKEFNESRWKSVFADATIPVERADDGTSFIQMDQALVGLGVALLISV"
     gene            1700..>2271
                     /locus_tag="VFQ54_01990"
     CDS             1700..>2271
                     /locus_tag="VFQ54_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU0163783.1"
                     /translation="MSNETWQGTRITESGVEFAVWAPGARAVELEFINDEGPWYVSLD
                     RQENGVWTADVAEAAAGTRYKYRVDGGYPCPDPRSRFQPDGVHGPSEVVDPGAYGWTD
                     QAWPGIQAENQVFYELHVGTYTGDGTFAALERELAELKRLGITAIELMPIAQSPGERN
                     WGYDGVDLFAPSSIYGRPDDLRSLVDAAHR"
BASE COUNT          418 a          705 c          755 g          393 t
ORIGIN      
        1 cacggtcggc tacgagttcg cggcgtcggt gtcgggtctc ttcgtcgatg cccaggcgga
       61 gtcgctcttc aatcggatct acggaaggtt cacgggcgac gatattcgtt acggcgaact
      121 cgtatacgag atgaagctgc gccagattcg tgaggcattc gccagcgaac tcaacgtcct
      181 ggtcaacatg ctcggcaggc tctccgaaca cgatcggcac tcgcgggact tcacgaatgc
      241 ggagttccgg gaggtgctgc gggagacgat tgcctgcttc gcggtctacc gaacctacgt
      301 cacgttcgac gatgtggggg ccgccgatcg cgaccgccgg gcgatccaat ccgcgatcgc
      361 gatggcggca cggaagcggg gcgcgatcga tcgcacggtg ttcagctttc tcgagcagac
      421 gttgctcgcg gagggaccgg tagacgatga ggtcacccgg cagcgacgcg cgttcacgat
      481 gaagctccag cagttgaccg gacccgtgat ggcaaaggga gtcgaggata ccgccttcta
      541 tcgcttcaac cgtctcgtct cgctgaacga ggttggcgga gaccccagcc ggttcggggc
      601 cagcgtcgag gacttccacc gccagaaccg ggctcgtctc aggagctggc ctgacggcct
      661 gctcgccggc tcgacgcacg acacgaaacg tggcgaggat gtgcgggcgt ggatcagcgt
      721 gctcagcgag attccgaccc agtggagagc ggcgctcaac cgctggagcc ggctcaaccg
      781 caaactgaag gtgaagcgcg agggaatcct cctgccccac cgcgtcgacg agtatgtgat
      841 ctaccagacg ctgatcggga tctggccgcc ggaatcgatg tcgaatgccg agcggaccgc
      901 gttgggaacg cggctgggcg actacatgat caaggtcgcc cgcgaagcga gccgggcgac
      961 gagctggatc gatcccgatc agggctacga ggatgcgctc tccggcttca tccaggggtt
     1021 gctcgaacga cgacgctcgc ggctgttcct cgaggatttc gagggcttca tggaacgcgt
     1081 ccggcccgcc ggaatgctga acgcactcgg gcagcatctc cttcgcctga ccgcgccggg
     1141 cgtgccggac atctatcagg gaacggaact gtgggatgat agcctggtcg acccggacaa
     1201 ccggcgaccg gtcgatttct cgatcagggc gacgaacgcc caggcattcc cccagatggc
     1261 gtcgcccgcc atgacactcg cgaaccccgc agtcaagcaa ttcgtgacag ccaggacgct
     1321 cgcagtgcga gccgcccacc cggacctgtt cgcgcgggga gcatatcttc ccctcatcgt
     1381 cgaaggcccc ggtgccgagc atgtcgtcgc attcgctcgc accgccaccg atgcctgcgc
     1441 gatcgtgatc gttccccggc tcgtcctcgg attcggtggc ggtgcgtccg atgccggaat
     1501 ccgggcggga aacacgctca cgcttccgaa agaattcaat gagtcgagat ggaagagcgt
     1561 ctttgccgat gcgacgattc ccgtcgagcg ggccgatgac gggacatcgt tcatccagat
     1621 ggatcaggca ctggtggggc tgggcgtggc gctcctgatc tccgtgtgat tgaaccgact
     1681 gtgggagaag agggaatcaa tgtccaacga gacctggcag ggtacgagaa tcacggaaag
     1741 cggcgtcgag ttcgccgtct gggcgcccgg agcgcgggcg gtagagctcg aattcatcaa
     1801 cgatgagggc ccctggtatg tgtcgctgga ccgccaggag aacggggtct ggacagccga
     1861 cgtggcggaa gcggcagccg ggacacgcta caagtatcgt gtcgacgggg gatatccgtg
     1921 tcccgatccc cgctcccggt ttcagccgga cggcgtccac ggtccatccg aagtcgtcga
     1981 tcccggcgcg tacggctgga ctgaccaggc gtggccgggc atacaggcgg agaatcaggt
     2041 cttctacgaa cttcatgtcg gcacctacac tggcgacggc acgttcgcgg ccctcgagcg
     2101 cgagcttgcc gaactcaagc gccttgggat caccgcgatc gagctgatgc cgatcgccca
     2161 gtctcccggt gagcggaact ggggctacga cggcgtcgat ctcttcgccc catcgagcat
     2221 ttacggtcgt cccgacgacc tgcgctcgct cgttgacgcg gcgcatcgga t
//
LOCUS       DASTMD010000126         1892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1082622, whole genome shotgun sequence.
ACCESSION   DASTMD010000126 DASTMD010000000
VERSION     DASTMD010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082622"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VFQ54_01995"
     CDS             <1..806
                     /locus_tag="VFQ54_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642236.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU0163784.1"
                     /translation="FRCGFLGLLHMEIIQERLEREYNLDLLATAPSVQYEVEMHSGEI
                     RKIDNPSDMPNLGEVNAIREPWMHLSIIAPSRYIGTIMDLATTRRGVFDEMTYLDEDR
                     VQIKYDIPLGELIVEFYDHLKSRTQGYASLDYTIAGMRAADLVKLDVLVNGQAVDALS
                     IITHRDDAYYKGRELVQTLRSLIPRQMFDVPVQASIGSRIISRETIKAMRKNVLAKCY
                     GGDISRKRKLLEKQKEGKSRMKMVGSVEIPQEAFMAVLSLGSGDKAAAK"
     gene            803..>1892
                     /locus_tag="VFQ54_02000"
     CDS             803..>1892
                     /locus_tag="VFQ54_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEU0163785.1"
                     /translation="MSSPGDARITIVGLGPGDPDRRTIAVQRALDAADRIVLRSFDHP
                     GLDDLIADPRVTDASSVSARIDGDTRDWAAGARYACDLAQSGNRVVVAAPGHPMYGER
                     LVIETLHEAAEHGIPTVVLDGLSTLDLSSSALAFDAMVDEVQIVDGAAIVHAIGDGPF
                     GGGQFPFTPLRPMLISRVYNQHITSRLQALLSRLFPTTHPITIVDAAGHPTEQAIREI
                     PLADLAQEPGARRLSLWVPPMDPLTAGRDPRTLQYIGARLRRPDGCPWDREQTHASLA
                     PSFVDEVYEVVDAIEAGHTANLAEELGDIYLHIVMQAQIAEEAGEFTLEDVFEGISAK
                     IVRRHPHVFGDDEVKSSIDVVGLWQRVKD"
BASE COUNT          354 a          602 c          606 g          330 t
ORIGIN      
        1 gcttccgctg cggtttcctc ggcctgctgc acatggagat catccaggag cgactggaac
       61 gcgaatacaa tctcgatctc ctcgcgaccg cgccgagcgt gcaatacgaa gtcgagatgc
      121 acagcggcga gatccgcaag atcgacaatc cgtcggacat gccgaatctc ggcgaggtga
      181 atgcgatccg ggagccatgg atgcatctca gcatcatcgc gccatcccgc tacatcggca
      241 cgatcatgga cctcgcgacg acgcgacgcg gcgtcttcga cgagatgacg tatctggacg
      301 aggatcgcgt ccagatcaaa tatgacatcc cgcttggcga actgatcgtc gagttctacg
      361 accacctcaa gagccgcacc cagggatacg catcgctcga ctacaccatc gccgggatgc
      421 gcgccgccga tctcgtcaag ctcgacgtgc tcgtgaacgg gcaggcggtc gacgcgctct
      481 cgatcatcac ccaccgcgac gatgcctact acaaggggcg ggagctcgtg cagacgttgc
      541 ggagcctgat cccgcgacag atgttcgacg tgccggtcca ggcatcgatc ggcagccgga
      601 tcatctcccg cgagacgatc aaggcgatgc gcaagaacgt tctcgcgaag tgctatggtg
      661 gcgacatcag ccgcaagcgc aagctcctcg agaagcagaa ggagggcaag tcgcgcatga
      721 agatggtcgg cagcgtggag atcccgcagg aagccttcat ggcggtcctc agcctcggat
      781 ccggcgacaa ggcggcggcg aagtgagttc cccgggggac gcgaggatca cgatcgtcgg
      841 actcggccct ggtgaccccg atcgccgcac gattgccgtg caacgcgcac tcgacgctgc
      901 ggatcggatc gtgctgcgct ccttcgatca tcccggtctc gacgatctga tcgcagatcc
      961 gcgcgtcacc gatgcaagtt cggtatcggc gcgtatcgac ggagataccc gcgactgggc
     1021 cgccggggca cgatatgcat gcgatctcgc gcagagtggt aacagggttg tcgtcgcggc
     1081 gccgggtcac ccgatgtacg gcgagcggct tgtgatcgag accctccatg aagccgcgga
     1141 gcacggcatc ccgacggtcg tattggacgg actcagtacg ctcgatctca gctcgtccgc
     1201 gctcgccttc gatgcgatgg tggacgaggt tcagatcgtc gacggcgcgg cgatcgtcca
     1261 cgcgattggc gacggcccgt tcggcggcgg ccagtttccc ttcacgccgc tgcgacccat
     1321 gctgatctcc cgtgtctaca accagcacat cacgagccgg cttcaggcgt tgctgtcgcg
     1381 gctcttcccc acgacacatc cgatcacgat agtcgatgcg gcgggtcatc cgaccgagca
     1441 agcgatccgt gagattcccc tcgccgatct ggcgcaggag ccgggtgcgc gtcgcttgtc
     1501 gctctgggtg ccgcctatgg atcccctgac cgcgggccgc gatcctcgaa cgctgcagta
     1561 catcggagcg cgactgcggc gacccgacgg ttgcccgtgg gatcgcgagc agacgcacgc
     1621 gtcgctggcg ccgtccttcg tggacgaggt ctacgaggtc gtcgatgcga tcgaggccgg
     1681 acacacggcg aatcttgccg aggagctggg tgatatctat cttcatatcg tgatgcaggc
     1741 gcagatcgcc gaggaagcgg gcgagttcac gctcgaggac gttttcgagg gcatctccgc
     1801 caagatcgtt cgccgccacc cccacgtctt cggcgacgac gaggtgaagt cctcgatcga
     1861 tgtcgtggga ctctggcagc gggtgaagga tg
//
LOCUS       DASTMD010000127         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3048035, whole genome shotgun sequence.
ACCESSION   DASTMD010000127 DASTMD010000000
VERSION     DASTMD010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3048035"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFQ54_02005"
     CDS             complement(<1..507)
                     /locus_tag="VFQ54_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163786.1"
                     /translation="MSSKMDESRDGTFDTEENHPSATLNRRKLIGTGAALSAGLAVSA
                     LAARAAGAQDAATPAAATPESQTEASGAQFPVATSLGDAIPPEFTGDPTNWPAESFNL
                     AATRYVTESKISSSTVSQLGVAWNLPIGVSATFGALVANPVIADGILFQQDAQSNVYA
                     VKRDTGEKV"
     gene            complement(814..1068)
                     /locus_tag="VFQ54_02010"
     CDS             complement(814..1068)
                     /locus_tag="VFQ54_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163787.1"
                     /translation="MVPSTYYMQPFAFHREAPERLVVRDEAGRWYLWAGDGSDEIVEV
                     PARLAHWMIDRREMQVLPAPHMWFERDSLPVRTQLPVFGD"
     gene            complement(1369..1773)
                     /locus_tag="VFQ54_02015"
     CDS             complement(1369..1773)
                     /locus_tag="VFQ54_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU0163788.1"
                     /translation="MAAIRSESDASVTSTSAEASDPRLEEDADILAELEMSMEHLDRH
                     SHSYAGDKAKLLARLRRIEGQVRGVARMIEDDKYCIDVLTQISAVMASSRAVGMLVLQ
                     DHIRGCVIDGPPEEREDRIEELNLAIERFTRI"
     gene            complement(1931..2545)
                     /locus_tag="VFQ54_02020"
     CDS             complement(1931..2545)
                     /locus_tag="VFQ54_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEU0163789.1"
                     /translation="MKLKRADPAFFTLFERAATFVVEASDALEVLFASEIIDAEAFAL
                     LDEIEHKADSNTHDLLSLLERGAEPPLSRRNTRRLINEIDSIVDSAEEAGELALLCRV
                     GRATPIAHEMTVVLAKTAREISSLVGYLNGGTGYRPYVARIHEYEHEGDDLWMRAFGD
                     LFTGEVEPLDIIRWKEIYARIEDAIDRCEETAKFIERALAGTTD"
BASE COUNT          518 a          848 c          871 g          548 t
ORIGIN      
        1 gaccttctcg ccggtatccc gcttcactgc gtagacattg ctctgggcgt cctgctggaa
       61 gaggatgcca tcggcgatga ccgggttggc gacgagggcg ccgaacgtgg cgctcacacc
      121 gatcggcagg ttccacgcga cgccgagctg gctgacggtg ctgctgctga tcttgctctc
      181 ggtgacgtag cgcgtcgcgg cgaggttgaa gctctcggcc ggccagttcg tcggatcacc
      241 ggtgaactcg ggcgggatcg cgtcgccaag cgaagtcgca accgggaact gcgcgccgga
      301 agcctcggtc tgcgactccg gggtcgcggc ggccggggta gcggcatcct gggcgcccgc
      361 ggcgcgggca gcgagcgcgc tgacggcgag accggcgctc aatgcggcgc ccgtcccgat
      421 cagcttgcgc cggttcaggg ttgccgaggg atgattctcc tcggtatcaa acgttccgtc
      481 acgcgactcg tccatctttg acgacatagc ctgttgactc ctcgatcgtc gagacctggc
      541 tcggcgttat ggtttgtaga ttcggcggcg tcgctcgccg aatttgtaaa agacgcctgt
      601 ctattatcag gcttgtttcg tacacagcaa gtactatttt tcgaaccatg cacgtatgcc
      661 caaccgctga ctaccttctc ccgaaaagat ggcggcaaga gcgtgccatc agggggtcgt
      721 gggcagcaag aagccggatg aaccgtgacg tccatccggc tttcgcttcg ggtgcgggga
      781 ggctggggag tacgatggca aggtcccgaa agatcagtct ccgaagacag gcaattgcgt
      841 tcggaccggc agggaatcac gctcgaacca catgtgcggc gcgggaagaa cctgcatctc
      901 ccggcggtcg atcatccagt gggcaagcct ggcgggaact tcgacgatct cgtcgctgcc
      961 gtcgcctgcc cagagatacc agcgaccggc ctcatcgcga acgaccagtc gttccggcgc
     1021 ctcgcggtgg aaggcaaatg gctgcatgta atacgtgctg gggaccattg atacccaaac
     1081 ctttcaagac aaagatggaa ctgggaggac aaatttggat cgtttagtcc cagagacccg
     1141 atgtttgagg tgtcttggca ccacgaacga aaatccggtt tgcaatctct gtgccacaac
     1201 atgatgtacg gtcatcacgc agcggtagac gccttcatct catgcgtatg caatctcgag
     1261 gagaacgatt tggggatcca gatcgggagg ttgccatggc acaacgacgc gtcacgtcat
     1321 tgagcgaaca tgcgagccca gtatatccca ccatttgcca tacgtgaact agatacgtgt
     1381 aaagcgttcg atcgcgagat tgagttcttc gatccgatct tcacgttctt ccggcggacc
     1441 gtcgatcacg cagccgcgga tgtggtcctg gagcacgagc atgccgactg cccgtgacga
     1501 cgccatgacc gcggagatct gcgtcagcac atcgatgcag tacttgtcgt cctcgatcat
     1561 gcgggcaacg ccgcgaacct gaccctcgat ccggcggaga cgggcgagca gttttgcctt
     1621 gtcacccgcg taggagtggc tgtgacgatc caggtgctcc attgacatct cgagttcggc
     1681 gaggatatcg gcatcctcct ccagccgggg atcgcttgcc tcggcggagg tcgaagtcac
     1741 tgaagcatcc gattcgcttc tgatggcggc cattagggga aagctcctta ttcattgctc
     1801 cggaaaggcg gggatgaggc ttgaatgctc cgggtgcgat cggccgacgc ccatatccgg
     1861 cacgccgggc cggcatcttc attatgacgt cttccgaatc ctccggctac cgaccgcgcc
     1921 agccgaacgg tcagtcggtc gtgccggcga gcgcccgctc gatgaacttc gcagtttcct
     1981 cgcagcgatc gatcgcatcc tcgatccgcg cgtagatctc cttccagcgg atgatgtcga
     2041 gcggctcgac ctcgccggtg aagaggtcgc cgaacgcgcg catccagaga tcatcgcctt
     2101 catgctcgta ctcgtggatc cgcgcgacat acgggcgata gccggtgccg ccgttgaggt
     2161 aacccacgag cgacgagatc tcgcgcgccg tcttcgcaag cacgaccgtc atctcgtgtg
     2221 cgatcggcgt cgcccgcccg acgcgacaga gaagggcgag ttctcccgcc tcctccgcgg
     2281 agtcgacgat cgagtcgatc tcgttgatga gtcgtcgcgt gttccggcgc gagagcggtg
     2341 gctcggcgcc acgctcgagc agggagagga gatcgtgcgt gttgctgtcg gccttgtgct
     2401 cgatctcatc gagcagggcg aacgcctcgg cgtcgatgat ctcggaggcg aagagcacct
     2461 ccagggcatc ggaagcctcg acgacgaagg tagcggcacg ttcgaagagg gtgaagaagg
     2521 ccggatcggc ccttttgagc ttcatccagg atttcggcat cgtgttcgct cccatgtgcg
     2581 cgtcatacgt tacgtccagt ttgccacatt cccccttttg cggcggtacg cacgacacct
     2641 tcccgaacac gtcctcccga gcctgtcgag ggaccagctg gcgtccacgt gcttcccgca
     2701 cgacgccgcc ccgaacggcg acaagcgccg tggggacggg aatgagtacg tgccgtgcgt
     2761 ataacgcctc ctcggatacg tcctc
//
LOCUS       DASTMD010000128         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1440647, whole genome shotgun sequence.
ACCESSION   DASTMD010000128 DASTMD010000000
VERSION     DASTMD010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440647"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..945)
                     /locus_tag="VFQ54_02025"
     CDS             complement(154..945)
                     /locus_tag="VFQ54_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163790.1"
                     /translation="MELSDVRTNQAIGAWLRAAGGNPEPLTTTEFDEPVWVARFGGDK
                     LPAIVITAGSHADEVAGVFAALELARDLKTDRAVYVIPCRDPLGWNGFAATLAQAAPD
                     AGSVGSYEEAAAVLRDGDVIFDDGSFVIARVGDLTFATEPSGKWTSTQITRHKLPRML
                     REDPELAAKLRNTRIIVPGNVETNDGRTPYTYGGHTAYIGDGFFAQLNRFFDREDGPV
                     EVTALRDFVDRIKPGLTLDLHEVSPPATTSSCTSPVPRKPTSWPA"
     gene            complement(958..1734)
                     /locus_tag="VFQ54_02030"
     CDS             complement(958..1734)
                     /locus_tag="VFQ54_02030"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0163791.1"
                     /translation="MIIDFHAHARRDQGDPANLINAMDENGVDLMVLHPIVPTAAGVG
                     ESSNEFIAELVARYPDRFAAFACVQPLEESAPEELKRLVETYGFKGLKLHPPIQQFEL
                     SNSAIYPTLRMAEQLELPVLIHTGPIFPRGARLAYGDPLPADDLAYIFPDLDMIIAHA
                     DPFGHQPTIAGRHPRVYTDTAVVFTRFARLIPGVGEDMLDWMSIGGQPGYTKLLFGTD
                     ANPRNTLRLSQTIDAIKAMDIDDEQKAAILGGTAQKLLRL"
     gene            complement(1731..>2121)
                     /locus_tag="VFQ54_02035"
     CDS             complement(1731..>2121)
                     /locus_tag="VFQ54_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0163792.1"
                     /translation="GVQICYDISFPEVSRVLALKGVEINLVLSAGPDEFRESWGPLLR
                     VRSSENAFWSVYANTVGLQKETNFFGGSRIVGPDGSTRTMGPFDEEACVIGEIDLHES
                     RMQRRSTLRFRERVPSLYRPITDGELA"
BASE COUNT          367 a          639 c          728 g          387 t
ORIGIN      
        1 cgaacgaggc gtgcgcatcg gcgcccggag gacccatcgt gaagatcccc tcccccttcg
       61 agaggatgcc ggcggcggtc gccgggcccc agacgggttc gagctcctcc cgggtcgcga
      121 tcgcaccgcc gctcgcgcgg acggcgtcga tcatcacgct ggccagctcg taggtttccg
      181 ggggactgga gaagtgcacg aagaggtagt agctggagga gaaacctcgt gcaggtcaag
      241 cgtgagcccc ggcttgatcc gatcgacgaa gtcgcgaagc gccgtgacct cgaccgggcc
      301 gtcctcgcga tcgaagaagc gattcagctg ggcgaagaag ccgtcaccga tgtaggcggt
      361 gtgaccgcca taggtgtatg gcgtgcggcc atcgttggtc tcgacgttgc cgggaacgat
      421 gatgcgggta ttccgcagct tcgcggcgag ttcgggatcc tcccgcagca tccgcggcaa
      481 cttgtgccgc gtgatctggg tggatgtcca cttcccgctc ggctcggtcg cgaaggtgag
      541 atcgccaacc cgggcgatca cgaacgagcc gtcgtcgaag atcacgtcgc catcgcggag
      601 gactgccgcg gcttcctcgt acgaaccaac gctgccggcg tcgggcgccg cctgagcgag
      661 ggtcgcggcg aagccgttcc agccgagcgg atcccggcag gggatgacgt agaccgctcg
      721 gtccgtcttc aggtcacgcg cgagttcgag cgcggcgaaa acgccggcca cttcgtcggc
      781 gtggctgccg gcggtaatca cgatcgcggg gagcttgtcc ccgccaaacc tggcgaccca
      841 gaccggttca tcgaattcgg ttgtcgtcag tggctcagga ttgccccccg ccgctcgcag
      901 ccatgcgccg atggcctgat tggtgcggac atctgagagt tccatcgatc ttctcctcta
      961 gagacgaagc agcttctgtg cggtaccgcc gagaatggcg gccttctgct cgtcgtcgat
     1021 atccatcgcc ttgatcgcgt cgatggtctg cgagagtcgg agggtattcc ggggattggc
     1081 atcggttccg aagagcaact tcgtgtagcc gggttggcca ccgatgctca tccagtcgag
     1141 catgtcctca ccgacgcccg ggatcagtcg tgcgaaccgg gtgaagacga ccgcggtgtc
     1201 ggtgtagacc cgtggatggc gcccggcgat cgtcggttgg tgaccgaagg gatcggcgtg
     1261 agcgatgatc atgtcgagat cggggaagat gtaggcgaga tcgtcggcag gcaggggatc
     1321 gccgtaggcg agccgggcgc cgcgcggaaa gatcgggccg gtgtgaatga gtacgggaag
     1381 ctcgagctgt tccgccatgc gcagtgttgg atagatcgcg gaattgctga gctcgaactg
     1441 ctggatcggt gggtgaagct tcaggccctt gaatccgtag gtctcgacga gccgcttgag
     1501 ttcttccggc gccgattctt ccaggggttg cacgcaggcg aacgcggcga agcgatccgg
     1561 gtatctggcg accagctccg cgatgaattc gttcgaggat tcaccaaccc cggcggcggt
     1621 cggcacgatc gggtgcagca ccatcaggtc gacgccgttc tcgtccatcg cgttgatcag
     1681 attggccgga tctccctggt cgcgacgtgc gtgggcgtga aagtcgatga tcatgcaagt
     1741 tctccgtcgg tgattggtcg atagagggag ggaacgcgct cccggaagcg caacgtcgac
     1801 cgtcgttgca tccgggattc gtggagatcg atctcgccga tcacgcaggc ctcctcgtcg
     1861 aacgggccca tcgtgcgtgt cgatccgtcc ggccccacga tccggctgcc accgaagaag
     1921 ttggtctcct tctggagccc gaccgtattc gcgtagacgc tccagaaggc attctcggaa
     1981 ctgcggacgc ggagaagcgg tccccagctc tcccggaatt cgtccggccc ggccgagagc
     2041 acgaggttga tctcgacgcc cttgagcgcg aggacgcgac tgacctcggg gaaactgatg
     2101 tcgtagcaga tctggacacc g
//
LOCUS       DASTMD010000129         1613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1440746, whole genome shotgun sequence.
ACCESSION   DASTMD010000129 DASTMD010000000
VERSION     DASTMD010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440746"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..148)
                     /locus_tag="VFQ54_02040"
     tRNA            complement(75..148)
                     /locus_tag="VFQ54_02040"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Glu,seq:ctc)
     gene            complement(234..875)
                     /gene="pdxH"
                     /locus_tag="VFQ54_02045"
     CDS             complement(234..875)
                     /gene="pdxH"
                     /locus_tag="VFQ54_02045"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEU0163793.1"
                     /translation="MNALSDMRQNYRKGELVEDHVQPDPIAQFGIWFDEVRSTDILEA
                     NAVIVATVGADGAPSARTVLLKSFDHEGFVFYTGYESQKGREIAQNPAVALTFYWPAL
                     ERQVRINGDAVRIAPELSDAYFATRPPGSQLGAVASPQSEVIPDRDWLANRVAEVSAD
                     IDDEHPLRRPETWGGYRVVPHTVEFWQGRPDRLHDRLRYRQVEGGWVIERLAP"
     gene            1085..>1613
                     /locus_tag="VFQ54_02050"
     CDS             1085..>1613
                     /locus_tag="VFQ54_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163794.1"
                     /translation="MERRPNSHWRGTAAKRVLVSSALAGSLLLTVLDATGTAVGAAAQ
                     TASPSPAVATCSLDPVTLPLFDATPPGQIVAQSSPAASPSAGGVTRPASSAEQAELTS
                     AIDVIVACMNTGNPAQIYGIFSDRYLAAMFADPAKAYLPAFEQSLAGPTIPEVPPYTL
                     TSADDFSFLENGSAEL"
BASE COUNT          271 a          529 c          508 g          305 t
ORIGIN      
        1 gctgctgcaa ccccgcaact cgatctcaaa gcaatacaca aaaccccttc caaatggaag
       61 gggtttcgac gttgtagcgc ataggggatt cgaacccctg gtctccgcct tgagagggcg
      121 gtgtcctagg ccgctagacg aatgcgccgg gtcgctttcg ggcaacgggc gatagattac
      181 catgcgtgga acctcccgtc aactcgggcg ttttcgggcc ggggcgcttc gctctacggt
      241 gcgagacgct cgatgaccca tcctccctcg acctgccggt agcgcagccg atcgtggagt
      301 cgatccgggc gcccctgcca gaactcgacc gtgtgcggga cgacgcggta tccgccccag
      361 gtctcggggc gtcgcagcgg atgttcgtcg tcgatgtccg ccgaaacctc ggcgacccga
      421 ttcgccagcc aatcgcgatc cgggatcacc tcgctctggg gtgaggcgac cgcaccaagc
      481 tggctccccg ggggtcgcgt cgcgaagtag gcgtcggaca gttcaggggc gatcctcacg
      541 gcatccccgt tgatccgcac ctgtcgctcg agtgccggcc aatagaaggt gagcgcgacc
      601 gcgggattct gtgcgatctc gcgtcccttc tggctctcgt agccggtgta gaagacgaat
      661 ccctcgtggt cgaatgactt gaggaggacc gtgcgggcag atggcgcgcc gtcggcgcca
      721 accgtggcga cgatcaccgc gttcgcctcg aggatgtcag tgctccggac ctcatcgaac
      781 catatcccga attgggcgat cgggtcgggt tggacgtggt cctcgaccag ctcgcccttg
      841 cggtaattct gtcgcatgtc ggaaagcgcg ttcattgtga cgtgatccag gtgaatgtgg
      901 cgtgggagcc attccgtcga tatcgtccgg ctgtatgatg acgaagcccg gcccggcatc
      961 cgcgggccca gctactcccg gtaggatacg cgacgatcat cccggatcgt gctcttcatc
     1021 gtctcgtttg tcaccaacgg gtggccgcca ggaacaatga tttgaccgag tggaggaact
     1081 cctgatggaa cgacgtccga attcgcattg gcgcgggacc gccgcgaagc gtgttctcgt
     1141 gtcctcggcg ctggcgggaa gtctcctgct gaccgtactc gatgcgaccg ggacggcggt
     1201 cggcgccgcc gcacagacag cgtcgccatc gcccgccgtt gcgacatgct cgctcgatcc
     1261 cgtgacgttg ccgctcttcg acgcgacacc accgggtcag atcgtcgccc agtcttcccc
     1321 ggctgcctca ccgtcggcag ggggagtcac gcgtccggcc tcgtccgccg agcaagccga
     1381 gctcacgtcg gcgatcgacg tcatcgtggc ctgcatgaat accgggaatc cggcgcagat
     1441 ctacgggatc ttcagcgatc gctaccttgc cgcgatgttc gccgatccgg cgaaagcgta
     1501 cctgcctgcc ttcgagcagt cactcgccgg gccgacgatc ccggaggtac cgccctatac
     1561 cctgacctcc gccgatgact tcagttttct ggagaacggg agcgccgaac tca
//
LOCUS       DASTMD010000130         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_280387, whole genome shotgun sequence.
ACCESSION   DASTMD010000130 DASTMD010000000
VERSION     DASTMD010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280387"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..388
                     /locus_tag="VFQ54_02055"
     CDS             104..388
                     /locus_tag="VFQ54_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163795.1"
                     /translation="MTDAQREEQIQLAREIGEELTEAFIAYVTGEVAFDDLTFGVYDA
                     LSDLHVIASGDYELEDEASEASASDVVDTTFPGDDEDIVTAEQPRDIPSS"
     gene            423..1214
                     /locus_tag="VFQ54_02060"
     CDS             423..1214
                     /locus_tag="VFQ54_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163796.1"
                     /translation="MSDISGAHHQRRRQRRVRNTLIVIVVIFVIVAVPLAINDQLRLD
                     VAYKVGMAPGGSIERVAQGDGVESLIVIPFKAKEDTAANSLYRSRAQYLATAGADSTT
                     LFNIVTGKTVTIPLTSLDYIAASNDGSKVFIRQGDAPSAYASVLLDTRTDKVDSLPAG
                     ASAPTIAGDWTTPIWSKTNGRCGPRSVTGKYIACFPRSHLAQYLAGDWQLNIQVYGDY
                     RQHEDLFRGLGFLPTVGWNDDDSEVWFANEKGIWRAKIDPSKFSI"
     gene            complement(1224..1724)
                     /locus_tag="VFQ54_02065"
     CDS             complement(1224..1724)
                     /locus_tag="VFQ54_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU0163797.1"
                     /translation="MSETIDPAIRKRVLREFTYGLYAVSAIHDGERGIFTANWLSQVS
                     FDPLLVAVSIERESSTLPLIEASGRFAVATFRSDQRDLAAALGKPKVRAGDKFAALDL
                     EIVETASGIPVPGNLLGYVECALVSVTGAGDSVVVIGAVTEVRSLAEEDAAPLTMREA
                     GFRHAG"
     gene            2015..2563
                     /locus_tag="VFQ54_02070"
     CDS             2015..2563
                     /locus_tag="VFQ54_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163798.1"
                     /translation="MQTLLTILLAISVLAGLLRLARPVGMRIEGEVFLRTSVPLLARL
                     RMHAATSVAGLILALVLIVAFGMAPLIVLVPIVSYLILAAIPVTYTLTTAGIRVGWTR
                     FRRWTEFAGVSRARGGASLQGVHGSRSLRIWLSGSRGDDEFLHLLRHAIKQGYKGQNR
                     TEIVSFGDSAERMADDPTIITI"
BASE COUNT          495 a          876 c          794 g          492 t
ORIGIN      
        1 aaccgactcg tatgcggagg ctggcatcat cccggctcca ccttatgtat gttacttgga
       61 tgtgatttca acggcgcgtg cccatgcgat caggagatag atcatgacag acgctcagcg
      121 ggaagagcag attcagcttg cccgcgaaat cggcgaagag ctcacagagg cgttcattgc
      181 gtacgtcacc ggcgaggtcg cgttcgatga cctcactttt ggcgtctatg acgccctcag
      241 cgatctccac gtgatcgcaa gcggggatta cgaactcgag gatgaggctt cggaggcgtc
      301 ggcatcggac gtcgtcgaca cgacctttcc cggcgatgat gaagacatcg tgaccgcgga
      361 acagccgcgc gacatcccga gctcgtgagc gggacgggat cccgaccatg cgatcgccag
      421 gcatgagtga catcagcggg gcccatcatc agcgccgtcg ccaacgacgg gtgcgcaata
      481 cgctgatcgt catcgtggtg atcttcgtga tcgtggcggt cccccttgcc atcaacgacc
      541 agcttcgcct ggacgtcgcc tacaaggtcg gcatggcgcc tggaggcagc atcgaacggg
      601 tcgcccaggg cgatggcgtc gagtcgctca tcgtcattcc gttcaaggcg aaagaggata
      661 cggccgcaaa cagcctctat cgcagccgcg cccagtacct cgccaccgcg ggcgccgact
      721 cgacgaccct cttcaacatc gtgaccggca agacggttac gatccccctc acgtcactcg
      781 actacatcgc cgcatcgaac gatgggtcga aggtcttcat tcgtcagggc gacgcacctt
      841 cagcctacgc gtccgtgctc ctcgacacgc gcacagacaa ggtggattcc ctccctgccg
      901 gggcatcggc acccacgatc gccggcgact ggacgacgcc gatctggagc aagacgaatg
      961 gccggtgcgg cccacgatcc gtcaccggga agtacatcgc gtgcttcccc agatcccacc
     1021 tcgcgcaata tcttgccgga gactggcaac tgaacatcca ggtgtatggc gactaccgcc
     1081 agcacgagga tctcttccgc ggactcgggt tcctcccgac cgtcggctgg aacgacgacg
     1141 actccgaagt ctggttcgcg aacgagaagg ggatctggcg ggcgaagatc gacccgtcga
     1201 agttctcgat ctgacgcgac ctctcacccg gcgtgacgga atcctgcttc ccgcatcgtg
     1261 aggggagcag catcctcttc cgccagcgac cgcacctccg tgactgctcc gatcacgacc
     1321 acggaatcgc ctgccccggt gacggaaacc agcgcgcatt cgacgtaccc gaggagattc
     1381 ccgggaacgg gaatgccgct cgccgtctcg acgatctcga gatcgagcgc cgcgaacttg
     1441 tcgccggcac ggaccttcgg cttcccgagc gcggcggcga ggtcacgctg gtcggaacgg
     1501 aaggtcgcga ccgcgaatcg cccgctcgcc tcgatcaacg gcaatgtcga gctttcacgc
     1561 tcgatcgaaa ccgcgacgag cagcggatcg aacgagacct ggctgagcca gttcgccgtg
     1621 aagatgcccc gttcaccgtc gtggatcgcg gagaccgcgt agagtccata ggtgaactcc
     1681 cggaggacgc gcttacggat ggcgggatcg atcgtttcgg acattgactg cctccgttga
     1741 ccggatactc atggccgccg tgacgcgctg cccagcgttc gcgcgtttga tttgggcggg
     1801 cgaccaacag acgttcgggt ttgcctgccc gtctttatca ttgcgcaacc atcattatcc
     1861 gaatggcgta gtacgataga ggtgcgggca gacatcaccc gcacatgtca cctttacgca
     1921 ggtgatgatg tttcccggcg cacccgcacc gatgacaacg aaatctccgc cgggtttcca
     1981 gctcttcccc tttttgcaac ccggatttga tcgaatgcaa acccttttga caattctcct
     2041 cgcgatcagc gtccttgccg gattgcttcg tcttgcacga ccggtcggca tgcggatcga
     2101 aggcgaggtc tttcttcgaa cgtcagtgcc gctgctggcc cgcctgcgca tgcacgccgc
     2161 gacatcggtc gcggggctca tcctggcgct cgtgttgatc gtcgccttcg ggatggcgcc
     2221 gctcatcgtt ctcgtgccga tcgtctccta cctcatactc gcggcgatcc cggtcaccta
     2281 caccctgacg accgccggca tccgcgtcgg ctggacgcgg tttcgccgct ggacggagtt
     2341 cgccggggtc agccgtgccc gcggcggcgc cagtctccag ggcgtccacg gaagccggag
     2401 cctgcggatc tggctctcgg gatcgcgcgg tgatgacgag ttcctgcatc tcctgcgcca
     2461 tgcgatcaag cagggataca agggtcagaa ccggaccgag atcgtcagct tcggcgattc
     2521 cgccgagcga atggcggacg atccgacgat catcaccatc tagcacgaca tcccgttcag
     2581 catctggtcg ttcccagtgc gggaccgccg cagtccagac ggcctgtccc gcgcctagga
     2641 gacgacgccg tcgatca
//
LOCUS       DASTMD010000131         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_101779, whole genome shotgun sequence.
ACCESSION   DASTMD010000131 DASTMD010000000
VERSION     DASTMD010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101779"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VFQ54_02075"
     CDS             complement(<1..1155)
                     /locus_tag="VFQ54_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEU0163799.1"
                     /translation="MSFVPLVPIGDALGSLVDDRTASEQRAALAVLEDRLRSRRTEVH
                     DGWGAQYIERVHKKGKLTARERLAKLIDPGTRPFEVGTFVNYGEVFPGDQKSPGAGVV
                     TAFARIEGRWCMVIANDNTVASGAWWPRTPEKIERAQTMAMRLRIPTVYLVDCSGLFL
                     PEQSKSFPGALGAGHIFKMNSLLSAAGVPQIAGVFGDCIAGGGYMPIISDRVYMTEQA
                     YMVIAGAALIKGAKSQKLTSLDIGGPEVHVHRSGCADVRVPDDETLLISIRRDVARLP
                     SSGADFYRGDIGPIDPRFASAELAALIPSDHREAYDAHQVLARLCDQSLFWEVMPDLG
                     EEMICGVGRVNGLYAGFVINRQGLVEDPEHPGRRHPSGILYKQGIAKISAF"
     gene            complement(1182..1721)
                     /locus_tag="VFQ54_02080"
     CDS             complement(1182..1721)
                     /locus_tag="VFQ54_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163800.1"
                     /translation="MRLLVLVLVLGCDAGTPTTPAVTAIPVDAKPARPPMPVPREDEM
                     PKAIPEGMLVLTQVHGFTGSRSWLVIQRDGSVESGDRPHRYGKIPSERIAAIDALFRK
                     QRVKSPHYDDRISAQDAPTTWFNYVAPNGKVVSVVDRYASDPAVRAFENKLYALADSI
                     TWQDLPAAKSATSDLPPGR"
     gene            complement(1769..2245)
                     /locus_tag="VFQ54_02085"
     CDS             complement(1769..2245)
                     /locus_tag="VFQ54_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163801.1"
                     /translation="MKSILLMVVVACDAGEPKPGWNETPLTPSEAQRIPEGMLVLTHR
                     GGWNHDWTVFHRDGYVERLDRSGRGREVGLLLPGHIVKIDLLFLEHRVKSGNYIDQRY
                     VVDDGDSDRIEYEAPNGTRVVVDEYEHNAPQNVHDLYRDLDEIERSITWRNVPIAQ"
BASE COUNT          424 a          734 c          766 g          439 t
ORIGIN      
        1 gaacgcgctg atcttcgcga tgccctgctt gtagaggatg ccggacggat ggcggcggcc
       61 cggatgttcc ggatcctcga cgagaccctg gcgattgatc acgaatccgg cgtagaggcc
      121 gttcacgcga ccgacgccgc agatcatctc ttcgccgagg tcgggcatga cctcccagaa
      181 cagggactgg tcgcacaggc gtgcgaggac ctgatgggca tcgtaggcct cgcggtgatc
      241 gctcgggatc aaggcggcga gctctgcgga tgcgaagcgc ggatcgatcg ggccgatgtc
      301 accgcgatag aagtccgcgc cgctcgatgg caggcgcgcg acatcgcgac ggatcgagat
      361 caacagcgtt tcatcgtccg gaacccggac atccgcacaa ccggagcggt ggacgtgcac
      421 ctcgggtccg ccgatatcga gcgatgtcag cttctgcgac ttggcacctt tgatcagcgc
      481 cgcacccgcg atcaccatgt acgcctgctc ggtcatgtac acgcgatcgg agatgatcgg
      541 catgtagccg ccacccgcga tgcaatcgcc gaacacgccc gcgatctggg ggacgccggc
      601 ggcggacagg agcgagttca tcttgaagat gtggcctgca ccgagggcgc ccgggaagct
      661 cttcgattgc tcgggcagga acaggccact gcaatcgacg aggtagaccg tggggatgcg
      721 gaggcgcatc gccatggtct gcgcgcgttc gatcttttcg ggcgtccgcg gccaccatgc
      781 gccggaggcg acggtgttgt cgttcgcgat caccatgcac cagcggcctt cgatccgtgc
      841 gaacgcggtg acgacgccgg cgccgggcga cttctgatcg ccggggaaca cctcgccgta
      901 gttgacgaac gtgccgacct cgaacgggcg cgtgccagga tcgatcagct tcgcgaggcg
      961 ttcgcgggcg gtgagcttgc ccttcttgtg aacgcgctcg atgtactgcg cgccccaacc
     1021 atcgtgaacc tcggtgcgcc gtgatcgcag tcgatcttcg agcacggcca gcgcggcgcg
     1081 ttgctctgaa gcggtgcggt cgtcgacgag cgaaccgagc gcatccccga tcgggaccaa
     1141 cggcacgaac gacatgtgga gggcacccta ccacggcgat ttcacctacc gggcggaagg
     1201 tccgacgtag cgctcttggc ggctggaagg tcttgccagg tgatcgaatc agcgagcgcg
     1261 tagagtttgt tctcgaacgc gcggaccgct ggatcgctcg cgtatcggtc gactaccgat
     1321 acgacctttc cattcggagc cacgtagttg aaccaggttg ttggcgcgtc ttgggcggag
     1381 atccggtcat cgtagtgtgg gctcttcacc cgttgtttcc ggaacagggc atcgatcgcg
     1441 gcaattcgtt cgctaggaat cttgccgtac ctgtgcgggc ggtctccgct ctccaccgag
     1501 ccgtcgcgct ggatgacgag ccacgagcga gagcccgtga atccgtgaac ctgcgtgagt
     1561 accaacatgc cttccgggat ggctttcggc atctcgtcct cacgcgggac gggcatcggc
     1621 ggcctcgcag gcttggcgtc gaccggaatc gcagtgactg cgggagtcgt gggcgtgccc
     1681 gcgtcgcatc ccagcaccaa caccaacacc aacaaccgca tggatggaag aacggtagct
     1741 cggaacgacc tggaagcgaa cggcgatttc actgggcgat tggaacgttc cgccaggtga
     1801 tcgatcgttc gatctcgtcg aggtcgcgat acaggtcgtg aacgttctgt ggtgcgttat
     1861 gttcgtactc gtcgaccacc acgcgagtgc cgttgggagc ttcgtattcg atgcggtccg
     1921 aatcgccgtc gtcgacgacg taccgttggt cgatgtaatt cccactcttc acgcgatgct
     1981 cgaggaacag gaggtcgatt ttcacgatgt gaccgggaag cagcaggcct acttcacgcc
     2041 cgcgaccgct ccgatccaaa cgttcgacgt agccatcgcg gtggaagacc gtccaatcat
     2101 gattccaccc accgcgatgg gtcaggacca gcatcccttc cgggattcgc tgcgcctcgg
     2161 atggggtgag cggagtttcg ttccagcccg gcttcggctc gcccgcgtca cacgcgacga
     2221 ccaccatcag caagatcgac ttcatctggc tgggacgtcc caccaggtga tcgaatgttc
     2281 gagctcgagc agatcgcgtt cgagatctcg aacattctgc ggcgccgctt ccgcgtggtc
     2341 acgcagcgag acgtgcgtgc cgt
//
LOCUS       DASTMD010000132         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_459085, whole genome shotgun sequence.
ACCESSION   DASTMD010000132 DASTMD010000000
VERSION     DASTMD010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459085"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..1800
                     /locus_tag="VFQ54_02090"
     CDS             496..1800
                     /locus_tag="VFQ54_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163802.1"
                     /translation="MIATTLRSIRRLALMVPFLVAGFFATSAIGGGQAALATVACGTS
                     LGELVTNGGFETPVLDPDSQVFWPDGTGWTPSDPSQVLLTSYEASAGSQSAVSVGATI
                     SQTLDTTGLDGFTLWVSLDTATGGAITVGSGAPQAFGWNGGGWGPFTTSYVVGPGESS
                     VALVFGLAGGEGDQMNIDNVSVTYGMPCPTPTPTDTPTVTPTLVPTDTPTVTPTPAAT
                     ETSVATSTLIPTETPESTATAAPTSEATGTTTSVVTPTAGTTGDVALTVLTGDGGAIP
                     DSASICLADQCTVAGAAVSSAAASGSSFSFTGVASGAQSLTITNVAPYADLTATIDVV
                     ADTTTTTSVTLDPAAVPTEPGNATPITTVTAMPTAGVNPTQPGGGGNGGGAPSSGGGV
                     PVKMLPNTGTGSGGDSSSILILFGAMLLTVATAAIAWRRRYR"
     gene            2036..2431
                     /locus_tag="VFQ54_02095"
     CDS             2036..2431
                     /locus_tag="VFQ54_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU0163803.1"
                     /translation="MIDRSIPDSSNPATQSSGAAEAPGSDLSIAVSPSTTATPARPLV
                     RFYMTSDLRAATGLTRSHLDFYLREGVIVPTARTESGYLLFDETELSTLRQVIIWRQD
                     GVGIRDIRARLSREADETATPGVATQPND"
     gene            2530..>3193
                     /locus_tag="VFQ54_02100"
     CDS             2530..>3193
                     /locus_tag="VFQ54_02100"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0163804.1"
                     /translation="MSSAIPAPPVIRLAAIYRSLGLSEDPFAIGTIPADPAPLAPFVD
                     GRERLVDWIRDRRAGSPSLALVIGANGSGRSTLLADVITHLAKEPATRPIVVTPDAEK
                     TTDARLLRAIVHAFGGEPIGRTGLELVNEIRQLAIASLPDALPVIAIDGVEFTGSRLE
                     LLRSLLTPPADAPDGYDLRIVVTGNPDLSDRLARRRTLAHQVGRTIALAPLTNDEVGQ
                     LVR"
BASE COUNT          553 a         1128 c          957 g          555 t
ORIGIN      
        1 ccgccaggcg ggggctgttg gctcaccgac ggtcgtgaat gtacacgatc gccagcgcga
       61 tagggagagt ataccgatcc ccccggattc tggccgcgca tgatcgtgtc atgccgtccc
      121 gcacatcctg aacgtactcc cgttctccat tatgaacgaa cgagaacccc atgcgtttcg
      181 atgtcgcgcg atcccggcca ttccggcagc ggttggcatc cggaggtacc ccagacctta
      241 gcgaacgtgt tcccgggtgc tacgctccgg agcggggcat cggtgtcact ttttgcgaaa
      301 catcacggaa gcacggcaac ctccctgggc ggaaacccgt ccagggcgaa cgtttgccat
      361 acgcctgcca ggtgtccacg ttctcgagga ggtacgagtt cgtttcatcc tgaggaaccc
      421 agggaccgat cgtcatcttc gcggctccag ggctggctgc cgcgccactc gacacatgag
      481 cattgggaga agaatgtgat tgctacgaca ctgcgatcca tccgccggct ggcgctgatg
      541 gttccattcc ttgtggctgg tttctttgcc acatcggcga tcggtggtgg ccaggccgca
      601 ctcgcgaccg tcgcatgcgg cacgtcactc ggcgagctcg tgaccaacgg gggtttcgag
      661 acgccggtcc tcgatcccga ttcacaggta ttctggcccg atggaacagg ctggacgccg
      721 agcgacccca gccaggtgct cctgacgagc tacgaagcct ccgccggcag tcaatccgcg
      781 gtctcggttg gagctacgat ctcccagacc ctcgatacga ccgggcttga tggatttacc
      841 ctgtgggtct ccctcgacac cgccaccggt ggcgcgatca ccgtcggatc gggtgctccc
      901 caggcattcg gctggaatgg cggcgggtgg ggtcccttca cgacgagcta cgtcgttggc
      961 cccggcgaat catcggtcgc actcgtcttc ggtctcgccg gaggcgaggg cgatcagatg
     1021 aatatcgaca atgtctcggt cacctacggc atgccatgcc caaccccgac gcccaccgac
     1081 acgccaaccg ttaccccaac cctggttcca accgacacgc cgaccgttac tccaaccccg
     1141 gcggcaaccg agacttcagt cgcgacatcg acgctgattc cgaccgagac tccggaatca
     1201 accgcaaccg ccgcgccgac gtcggaagcc accggcacca cgacttcggt cgtaacgccg
     1261 acggcgggca ccacgggtga cgtcgccctg acggtgctca ccggcgatgg cggtgccatt
     1321 ccagattctg cttccatctg tcttgccgac cagtgcacgg tcgccggggc ggccgtgagt
     1381 tccgccgcgg catccggaag cagcttcagt ttcaccggcg tcgcttccgg tgctcaatcg
     1441 ctgacgatca cgaacgtagc cccctacgcc gatctcaccg cgacgatcga cgtggttgca
     1501 gacaccacca ccacgacgtc cgtcaccctc gatcccgcgg ccgtcccgac cgagccgggc
     1561 aacgccacgc cgatcacgac ggtgaccgcc atgccgacgg caggcgtcaa cccaacgcag
     1621 ccgggtggtg gtggcaacgg cggcggcgct ccgtccagcg gtgggggagt tccggtcaag
     1681 atgctcccga acaccggtac aggcagcggt ggcgactcaa gcagcatcct gatcctcttc
     1741 ggggcgatgc tgctgacggt ggcgacggcc gcgattgcct ggcgacgccg ctaccgctag
     1801 agcgaggtga ccgcgtcgct gttccatcca gatcggtctt cgctgcagga tccgcattcc
     1861 acgaggatag tcacgcgagg cgccttttcc ggatgaccca ccgggccagg attgcaccgc
     1921 tccttgtgac aattgggaaa gcgtttactt tcgattacgc gccgcctact atcgttgata
     1981 tgcaattgca taccacccgg gtttcttccg ggtggagacg tgaggggcgc ttttcgtgat
     2041 cgatcgcagc ataccggatt cctccaaccc agccacgcag tcatccggcg ccgccgaagc
     2101 tcccggcagc gacctctcga tcgccgtctc gccgtcgacg accgccacgc cagcgcgacc
     2161 gctcgtccgc ttctacatga cgagcgatct ccgtgccgcc accggtctta cccgcagcca
     2221 tctcgacttc tatcttcgcg aaggcgtcat cgtcccgacc gcccgtaccg agagtgggta
     2281 tctgctcttc gacgagaccg aactctcgac gcttcgccag gtcatcatct ggcgccagga
     2341 cggcgtcggc atccgcgata tccgcgcccg gctctcccgc gaggccgacg agacggcgac
     2401 gccaggcgtc gccacgcaac cgaacgattg acgcaatgac ccgaagccga cggatgaaat
     2461 gacgtgcagc aaccagcagc gccaccatca gcagggacga cgcgatcatc acgggaggga
     2521 atccacgtgg tgagttcagc gatcccggcg ccacccgtca tccgcctcgc ggccatctac
     2581 cgatcgctcg gtctctccga ggatccgttc gcgatcggca ccatccccgc ggatccggcc
     2641 cctctcgccc ccttcgtcga tggccgggaa aggctcgtcg actggatcag ggaccgtcgt
     2701 gccggcagcc catcgctcgc gctcgtgatc ggcgcgaacg gctccggccg ctcgacgctc
     2761 ctcgccgatg tcatcaccca cctcgcgaag gaaccggcga cgcgaccgat cgtcgtcacg
     2821 ccggacgcgg aaaagacgac cgatgcgcgg ctcctccgcg cgatcgtcca cgctttcggc
     2881 ggcgagccga tcggtcgcac cggactcgag ctcgtcaacg agatccgtca gctcgcaatc
     2941 gcaagccttc cggatgcgct tccggtgatc gcgatcgacg gcgtggagtt caccggctcc
     3001 cggctcgagc tgttgcgctc gctcctgacg ccgccggcgg atgcgccgga cggctatgac
     3061 ctgcggatcg tcgtgacggg caacccggat ctcagcgatc ggctcgcccg ccggcggacg
     3121 ctggcgcatc aggtcggacg tacgatcgca ctcgcacctc tcaccaacga tgaggtcggg
     3181 cagctggtgc gct
//
LOCUS       DASTMD010000133         1715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_12142, whole genome shotgun sequence.
ACCESSION   DASTMD010000133 DASTMD010000000
VERSION     DASTMD010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12142"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFQ54_02105"
     CDS             complement(<1..507)
                     /locus_tag="VFQ54_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0163805.1"
                     /translation="MPQLAVITDELSEDLDHALAVCKELDIHVVELRSVWNTNVVNMG
                     SDDLARLETTIRRAGVTVCAIASPFLKCHIHEDGAAAGPLHSATETPRSAQWDILASS
                     LDLADRLDAPMVRTFSFWRLEDPGSARDEILDVLRDATSRVATRGRQLALENEHSCNI
                     GSGEEAAWY"
     gene            complement(610..1182)
                     /locus_tag="VFQ54_02110"
     CDS             complement(610..1182)
                     /locus_tag="VFQ54_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU0163806.1"
                     /translation="MDITDSTIRETYPALFALNRWGNVTFTGFHLLAEWPPLDLIQSV
                     NVVPFVGDRCVAIFLWNGHAMLPGGTRELGETLEETARRELIEETGASFKTCFPFAYW
                     ESFSADPKPWRPFLAHPHFIRAVAWADVELDGLPTNPPDGEQIATVELLTRTEAEARF
                     LRDDRPELAAIYALAWDVRARSRNEETAAS"
     gene            complement(1206..>1715)
                     /locus_tag="VFQ54_02115"
     CDS             complement(1206..>1715)
                     /locus_tag="VFQ54_02115"
                     /inference="COORDINATES: protein motif:HMM:NF019903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0163807.1"
                     /translation="TAIIRTKADEFGVRIADDIIEYVASKDQTNIRELEGALNKILMM
                     AQLYNRPLSLQLAMEALTDAVGTNRRGTITTDDVLNAVMDSYRISEKDLIGRQRKREI
                     VMPRQVAMYLLREETESSLMDIGRALGGRDHTTILHGVEKIEQLLETDTQLRSQIMTI
                     RETLLTSRR"
BASE COUNT          287 a          536 c          563 g          329 t
ORIGIN      
        1 ataccaggcg gcttcctcgc cactcccgat gttgcaggag tgctcgttct ccagcgccag
       61 ttgccgtccc cgcgtcgcga cgcgggatgt ggcgtccctg aggacatcga ggatttcgtc
      121 ccgtgccgag cccggatcct cgagccgcca gaacgagaag gtccgtacca tcggcgcatc
      181 gagacgatcg gcgagatcga gcgagctcgc gaggatgtcc cactgcgcgg atcgcggcgt
      241 ttcggtcgcc gaatgcaacg gaccggcggc cgcgccatcc tcgtggatgt ggcacttcag
      301 aaagggcgac gcgatggcgc agacggtgac gccggccctc cggatcgtcg tttcgagccg
      361 ggcgagatcg tcgctcccca tgttgactac gttcgtgttc cagacgctgc ggagctcgac
      421 gacgtgaatg tcgagttcct tgcagacggc gagggcgtga tcgagatcct cgctgagttc
      481 atccgtgatg acggcaagtt gaggcacccg gtctcccttt cacgtggcga cggtgacggc
      541 atcagcgatg ttcggccgaa gccacggcat gctagtcagc cagtgagaag gcgtcaagac
      601 gaccgccgcc taggacgccg ccgtttcctc gttcctgctg cgggctcgta catcccaggc
      661 cagggcatag atcgccgcca actcgggacg gtcgtccctc agaaagcgcg cttcggcttc
      721 cgtcctcgtc agcaactcta cagtcgcgat ctgctcgcca tccggtggat tggttggcaa
      781 gccgtcgagc tcgacatccg cccaggctac ggcgcggatg aaatgcggat gagcaaggaa
      841 cgggcgccac ggtttggggt cggccgagaa gctctcccaa taggcgaacg gaaagcacgt
      901 cttgaaggat gcgccggttt cctcgatgag ctcgcggcgc gcggtctctt ccagcgtctc
      961 gccgagctcg cgcgtaccgc cgggcagcat cgcgtgaccg ttccacagga agatcgcgac
     1021 gcagcgatcg ccgacgaagg gaacgacgtt cacgctctgg atgagatcga gtggtggcca
     1081 ttcggcgaga agatggaacc ccgtgaacgt gacattgccc cagcggttga gggcgaagag
     1141 cgcagggtag gtttcgcgaa tcgtgctgtc ggtgatgtcc aacgaacttc ccccggatgc
     1201 cggcattagc ggcggctggt cagcaacgtc tcgcgaatcg tcatgatctg gctgcggagt
     1261 tgggtgtcgg tctccaggag ctgttcgatc ttctcgacgc cgtggagaat cgtcgtgtga
     1321 tcccgaccgc cgagcgcgcg accgatgtcc atgagggaac tctcggtctc ctcgcgcagg
     1381 agatacatcg cgacctgccg cggcatcacg atctcgcgct tgcgctggcg gccgatgaga
     1441 tccttctcgc tgatccggta gctgtccatc accgcattga ggacgtcgtc cgtggtgatc
     1501 gtgccccgcc ggttcgtgcc gacggcgtcg gtcagggcct ccatcgccag ttgcaggctg
     1561 agcgggcggt tgtagagctg ggccatcatc aggatcttgt tcagcgcgcc ctcgagctcg
     1621 cggatattcg tctggtcctt gctcgcgacg tactcgatga tgtcgtccgc gatgcggacc
     1681 ccgaactcgt ccgccttggt ccggatgatc gccgt
//
LOCUS       DASTMD010000134         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1172879, whole genome shotgun sequence.
ACCESSION   DASTMD010000134 DASTMD010000000
VERSION     DASTMD010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172879"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2140
                     /locus_tag="VFQ54_02120"
     CDS             <1..>2140
                     /locus_tag="VFQ54_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU0163808.1"
                     /translation="FGIAEFVERCKQRVWKFSERQTDQSKRLGYFMDWDNSYYTMSDE
                     NNYTIWHYLKTCYDRGWIYKGHDVMPWCPRCGTGLSEHEIVTEGYQDRTHLSIYAAFP
                     LVDEDASLLIWTTTPWTLAANVAVAVHPELTYVKVLQEDKVFYIAKDALARTVRGDHE
                     VLAELPGSDLVGKTYVGPFDELPANEGVNHRVIAWTEVSPDEGTGLVHNAPGCGKEDF
                     ALSKEHDLQVIGPIDESGIYLKGFDWLTGRAAQNVAKEIADNLREKGYLYRAENYTHR
                     YPVCWRCGTDLLFRLVDEWFISMNELRPQLKNVTEDIRWIPSFGKERELDWLEHMDDW
                     MISKKRYWGLALPIFDCQECGKFEVIGSETELKERAISGWEKFEGHTPHRPYVDEVKI
                     ACSGCGAPVSRIKDVGNPWLDAGIVAMSTLNYRHDREYWNEWYPADLISESFPGQFRN
                     WFYALITESTALTGSKPTKNVFSYALMRDEKGEEMHKSKGNAIWFDDAADEIGVDTMR
                     WMFSTVNPSTNILFGYHATDEVRRRFILPLWNSYAFFTTYARIDGWTPPVTPTPVAER
                     SLLDRWLLSRVHELTLTVRDRMSDFDVSTGPRAIENFVVEELSNWYIRRNRRRFWKPE
                     SDADKAAAYETLYEVLVIVTKLMAPFIPFVTESMYQNLVRSISDNQPESIHLCDYPVA
                     DEALIDRQLTREMSAVLAVVKLGRAARSEAN"
BASE COUNT          424 a          667 c          659 g          390 t
ORIGIN      
        1 ttcgggatcg cggaattcgt ggagcgctgc aagcaacgcg tctggaagtt ctccgagcgc
       61 cagaccgacc agtcgaagcg tctcggctac ttcatggact gggacaattc gtattacacg
      121 atgtccgacg agaacaacta cacgatctgg cactacctga agacctgtta cgaccgtggc
      181 tggatctaca agggccacga cgtgatgccg tggtgcccgc gctgcggtac cggcctctcc
      241 gagcacgaga tcgtgacgga gggttaccag gatcgcacgc atctttcgat ctatgccgcg
      301 ttcccgctgg tcgatgaaga cgcgtcgctc ctgatctgga ccacgacccc gtggacgctc
      361 gccgcgaatg tcgccgtcgc cgttcatccc gagttgacct acgtcaaggt ccttcaggag
      421 gacaaggtct tctacatcgc aaaggacgcg ctcgcacgga cggtacgtgg cgaccacgag
      481 gtgctcgccg agcttccggg aagcgacctg gtcggaaaga cctacgtcgg tcccttcgat
      541 gagctgccgg cgaacgaggg cgtcaatcat cgcgtcatcg cctggaccga ggtctcaccc
      601 gacgaaggaa ccggtctcgt ccataacgcc cccggttgcg gcaaggagga cttcgcgctc
      661 tcgaaggagc acgacctcca ggtcatcgga ccgatcgacg agtcgggcat ctacctgaag
      721 ggcttcgact ggctgaccgg ccgcgccgcg cagaatgtcg cgaaggagat cgccgacaac
      781 ctccgcgaga agggctatct ctaccgcgcc gagaactaca cccaccgcta tccggtctgc
      841 tggcgttgcg gcaccgatct gctcttccgc ctcgtcgacg agtggttcat ctcgatgaac
      901 gagctgcgtc cgcagctgaa gaacgtcacg gaagacatcc gctggatccc ctccttcggc
      961 aaggagcgtg aactcgactg gctcgagcac atggacgact ggatgatctc gaagaagcgg
     1021 tactggggtc ttgccctgcc gatcttcgat tgccaggagt gcggcaagtt cgaggtgatc
     1081 ggttcggaga ccgaactcaa ggaacgagcg atcagcggct gggagaagtt cgagggccac
     1141 acgccgcatc gaccttatgt cgatgaagtc aagattgcct gctccggttg cggcgctccg
     1201 gtctcacgga tcaaggatgt cggcaacccc tggctcgacg ccggcatcgt cgcgatgtcg
     1261 accctcaact atcggcacga tcgcgagtac tggaacgagt ggtacccggc cgacctgatc
     1321 tcggagagct tcccgggtca gttccgcaac tggttctacg cgctgatcac ggaaagcact
     1381 gcgctcactg gcagcaagcc gacgaagaac gtcttctcct acgccctcat gcgtgatgag
     1441 aagggcgagg agatgcacaa gagcaagggg aacgcgatct ggttcgacga tgcggcggac
     1501 gagatcggcg tcgacacgat gcgctggatg ttctcgacgg tcaacccgag cacgaatatc
     1561 ctcttcggct atcacgcgac ggacgaggtg cggcggcgct tcatccttcc gctctggaac
     1621 tcgtacgcct tcttcaccac ctatgcccgg atcgacggct ggacaccacc cgtcactccg
     1681 acgccggtcg cggaacgcag tctgctcgac cgctggctgc tctcccgcgt ccacgagttg
     1741 acgttgaccg ttcgcgaccg gatgtcggac ttcgatgtct cgacggggcc gcgcgcgatc
     1801 gagaacttcg tcgtcgagga actctcgaac tggtacatcc gccgcaatcg gcgccgattc
     1861 tggaagccgg agagcgacgc cgacaaggct gccgcgtatg agacgctcta tgaggtgctg
     1921 gtgatcgtca cgaagctgat ggcgcccttc atcccgttcg tgacggagtc gatgtaccag
     1981 aatctcgtcc gctcgatcag cgacaaccag ccggaatcga tccacctctg cgactatcca
     2041 gtcgccgacg aggcgttgat cgatcggcag ctgacccgcg agatgagcgc ggtgctcgcg
     2101 gtggtgaagc tgggacgtgc ggcgcgatcg gaggcgaaca
//
LOCUS       DASTMD010000135         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_548812, whole genome shotgun sequence.
ACCESSION   DASTMD010000135 DASTMD010000000
VERSION     DASTMD010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548812"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1848
                     /locus_tag="VFQ54_02125"
     CDS             <1..1848
                     /locus_tag="VFQ54_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0163809.1"
                     /translation="THPEAKDRTAIWLVPVQPLPAPVVAADEPSADAEATADESSDVA
                     AAAAPVETGDNVAESTTVASTDELPDEAIVAATETEDVEAVAAPEEEPVAEPAPVDER
                     VPVILSDHPTASDRSPRWSPDGQLLAFVRYLDGRDVIALASTDGAFPVAAEILTWSDS
                     HDREPAWSRDGLFLAFTRKLNIAPEHADIIVFVLASGEITNLTGEKTPAIRHSLEWVP
                     GRNLIAFVTRESEWLSIAVINSDNKAGWTVTREAGDKTDPRFNPKEARLVYIRNEGFT
                     SVICERGLHASGALALDPGEGVASFARWGEEKRVVYGFSAPQRPFGFLVQDNLVSNDR
                     IPVNIPGISLTAGQTFRQPSPFEFEVGPEEQFSGLLYRTSGVAGKVPAIVYMPEGPLT
                     TRRGGFYPEEQALASTAMAVLTPVIHGATGWGVDVERDLADYADTEIEISDIAEAGTK
                     LGEVSDIDGSKVAVVGTGYGGALALLAAGARPGIYSAIAAVDPITDWSIEFEEASASW
                     RNWVSRQYGMPLVERDRYAMRSPETFAGTIEVPMLLVSTKGAPRTRRTQLEAFMAYLD
                     EVGVTYDYIDAGDVSLPAALKQVGSWLASHYLEGRESVQVVEELRADDM"
BASE COUNT          338 a          705 c          701 g          333 t
ORIGIN      
        1 acacatccgg aggcgaagga ccgcacggcg atctggctcg tgccggtgca gccacttccc
       61 gcgccagtcg ttgccgccga tgagccgtcc gcggacgcgg aagcgaccgc cgacgaatcc
      121 tccgatgtgg ccgccgccgc ggctcccgtg gagaccggcg acaacgtcgc agaatcaacc
      181 accgtggcaa gtaccgacga attgccggac gaggcgatcg tcgctgcaac ggagaccgag
      241 gacgtcgagg ccgttgccgc gccggaggaa gagccggttg ccgaacccgc accggtcgac
      301 gagcgcgtgc cggtgatcct gagcgaccac ccgaccgcgt ccgatcgctc gccgcgctgg
      361 tcgccggacg gtcagttgct tgccttcgtg cgctatctcg acggccgcga cgtgatcgcg
      421 ctcgccagta ccgacggcgc cttcccggtc gccgccgaga tcctgacctg gtccgattcc
      481 cacgatcggg agccggcctg gtcgcgcgat ggcctcttcc tcgcgtttac gcggaagctc
      541 aacatcgcgc cggagcacgc ggacatcatc gtcttcgtgc tggcaagcgg cgagatcacg
      601 aacctgaccg gggaaaagac cccggcgatc cgccactcgt tggagtgggt gcccggtcgc
      661 aacctgatcg ccttcgtgac gcgggagagc gaatggctct cgatcgcggt catcaactcg
      721 gataacaagg ctggctggac ggtgacccgg gaagccggcg acaagaccga tccccgcttc
      781 aacccgaagg aagcccgcct cgtctacatc cggaacgagg gcttcacctc cgtcatctgc
      841 gagcgcggcc tgcacgcctc cggtgcgctc gcgctcgatc ccggcgaggg tgtcgcgagc
      901 ttcgcgcgct ggggcgagga gaagcgcgtt gtctacggct tcagcgcgcc gcagcgacca
      961 ttcggcttcc tcgtccagga taacctcgtc tccaacgatc gcatcccggt gaacattccc
     1021 ggcatctcgc tgacggccgg gcagaccttc cgtcagccgt cgccgttcga attcgaggtc
     1081 ggcccggagg agcagttctc cggtctcctc tatcgcacga gcggggtggc cggaaaggtg
     1141 cccgccatcg tctacatgcc ggagggaccg ctcacgacgc gtcgtggtgg cttctatccg
     1201 gaggagcagg cgctggcaag caccgcgatg gcggtgctga ccccggtgat ccacggcgcg
     1261 accggctggg gtgtcgatgt cgagcgggac ctcgctgact acgccgatac ggagattgaa
     1321 atttccgata tcgccgaggc cggcacgaag ctcggcgagg tcagcgacat cgacggcagc
     1381 aaggtggcgg tcgtgggcac cggctacggc ggcgccctgg cgctgctcgc ggcgggcgcc
     1441 cggcccggca tctactcggc gatcgcggcg gtcgacccga tcaccgactg gtcgatcgag
     1501 ttcgaggagg cgagtgcgtc gtggcggaac tgggtgtccc gccagtacgg gatgccgctg
     1561 gtcgagcgcg accgttacgc gatgcgttcc ccggagacct tcgccggaac gatcgaggtg
     1621 ccgatgctgc tggtttcgac gaagggcgcg ccgcgcactc gccggacgca gctcgaggcc
     1681 ttcatggcgt atctcgatga ggtcggcgtg acctacgact acatcgacgc tggtgacgtc
     1741 tcgctgccgg cggcgctgaa gcaggtgggg agctggcttg ccagccacta tctcgagggg
     1801 cgtgagagcg tccaggtggt cgaggagctg cgcgccgacg atatgtaggt atagagtggc
     1861 ggagatcaat caccacacga gccagacaag gagaggggcg gggtcacaac ggccctcgcc
     1921 cctctccttg ttcttcagga tggttccgcg gtagttctcc tcacccgtgc cccgggttac
     1981 tccgtcccct gggccccgac tgtccacccg atccgccaac cgtccacccg gttcgccaaa
     2041 ccgtcctccc gagcccgtcg agggagcagc cggcatc
//
LOCUS       DASTMD010000136         1780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3048516, whole genome shotgun sequence.
ACCESSION   DASTMD010000136 DASTMD010000000
VERSION     DASTMD010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3048516"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VFQ54_02130"
     CDS             complement(<1..947)
                     /locus_tag="VFQ54_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0163810.1"
                     /translation="MTIESPQVAEVRTILASHGLASEQLTTATGVVNNVWLTPELVVR
                     LNGGRFPDAFGHEARILDLLPATIPHPEAIAHGSRVEGGEYLVVTRVPGVTLDEAWPD
                     LDPDGRQRVIHELAAIVQTLHALPVAAGMRNPWIDDALTIPIPADAYHASPLFLPVLL
                     ASARNVRPDLATTVDQVKRLTDDRLDAFGDEPDVLVHADIHVRNILVDGEHISGLIDW
                     EGSRPGIADNELDTLIRFLGASRQVGSEQTNDYSGLIHWFREGYPELFAHPRLVDRLE
                     VYEAMWHLVQIHHWRPEYVTMVDPAAAFDDLLAGAFRAR"
     gene            1098..1454
                     /locus_tag="VFQ54_02135"
     CDS             1098..1454
                     /locus_tag="VFQ54_02135"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEU0163811.1"
                     /translation="MSETHEWMLADGMDVIGADGEKLGEVQEARGDYFIAGKGWLFST
                     EFYIPATAIVSADEQAVYLNVTKEQALGQGWDRPMQSATIINEGVYETFERPASGHDH
                     TPGGASPQAVAGDPRS"
BASE COUNT          324 a          545 c          590 g          321 t
ORIGIN      
        1 atccgcgccc ggaaggctcc ggcgagaaga tcgtcgaagg cggcggcggg atcgaccatc
       61 gtcacatact ccggacgcca gtgatggatc tggaccaagt gccacatcgc ctcgtagacc
      121 tcgaggcgat ccaccagccg gggatgggcg aagagctccg gatagccttc ccggaaccag
      181 tgaatgagcc ccgagtagtc gttcgtctgc tccgacccga cctgccggct ggcgccgagg
      241 aagcggatca gggtgtcgag ttcgttgtcc gcgatcccgg gacggcttcc ctcccagtcg
      301 atcaggcccg agatgtgctc gccatcgacg aggatgttgc ggacgtggat atcggcatgc
      361 acgaggacgt ccggctcgtc gccgaacgca tcgaggcgat cgtccgtcag tcgcttcacc
      421 tgatcgacgg tcgtggcgag gtctggacgc acgttccgcg cgctcgcgag gagcaccggc
      481 aggaataatg gtgacgcatg gtacgcatcg gcgggaatcg ggatcgtcag tgcgtcgtcg
      541 atccagggat tgcgcatccc ggcggcaacc ggtagcgcgt gcaacgtctg gacgatcgcg
      601 gcgagttcgt ggatgactcg ctgtcgtccg tccggatcga ggtccggcca tgcctcgtcg
      661 agcgtgacgc ccggcacgcg cgtgacgacg agatactcgc caccctccac gcgcgaaccg
      721 tgggcgatgg cctccggatg cgggatcgtc gccgggagca ggtccaggat tcgggcctcg
      781 tgaccgaatg cgtcggggaa gcggccgccg ttcaggcgca cgacgagttc cggcgtgagc
      841 cagacattgt tgacgacgcc ggtcgctgtg gtcagttgct cgctggcgag accgtggctg
      901 gcgaggatcg tccgcacttc agcgacctgc ggggattcga tcgtcatcgg agcccgccct
      961 gtggattctg gcgacgcatc attccctcct ttactcgcat gaaatgcctg gaacattgtc
     1021 cgttacgcca ttctggcatg tcgtctgcgt aaatcctggg atgaggcaga tgcagactca
     1081 ggcaaggaaa cccgacgatg agcgagaccc acgagtggat gctggcggac ggaatggacg
     1141 tgatcggcgc tgatggcgag aaactgggcg aagttcagga agcgcgcggc gactacttca
     1201 tcgccggcaa gggctggctc ttctcgaccg agttctacat cccggcgacg gcgatcgtca
     1261 gtgccgacga gcaggcagtc tatctcaacg tgacgaagga gcaggcgctc ggacaagggt
     1321 gggatcgtcc gatgcagagt gcgacgatca tcaacgaggg tgtctacgag acattcgaac
     1381 gccccgcctc gggtcatgac cacacgccgg gtggcgccag tccccaggca gtggcgggag
     1441 atccgagatc ctagaagcgc gatggtgacg catacatctc cggaagggac acctcaacgg
     1501 gtgtcctttt cgctgcggca cagagtgcct cgggacctgt tgacgcgatt gctatagtgg
     1561 aaagtcgcac gccgaacaat cgagtcgaag aagcgaaacg gtctgcgttc agtttgggag
     1621 gtctctgtcc tcgatggcta ccgacgcatt ctcccggagc agtgccgatc cgcgcgccct
     1681 ttcctccgcc cccgttcgcg tccgggttcg cagcatgctc cggctcgccg tctccctcgt
     1741 ctggacatcg tcaccgatca tgacgaccgt gctcttctcg
//
LOCUS       DASTMD010000137         1744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1173019, whole genome shotgun sequence.
ACCESSION   DASTMD010000137 DASTMD010000000
VERSION     DASTMD010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173019"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFQ54_02140"
     CDS             <1..240
                     /locus_tag="VFQ54_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163812.1"
                     /translation="MENPRVDAVTKERMRVWITEAETELVSRFGEGIGHSLVDAVRSG
                     WKSAKTEPALWEMKAGIREPETVADQNRYQWSAWS"
     gene            440..649
                     /locus_tag="VFQ54_02145"
     CDS             440..649
                     /locus_tag="VFQ54_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163813.1"
                     /translation="MISPQVKARRNINCPADFLFLAASLCMESARTAKRIHIDSPAAR
                     LVIAEHVKRARANNHRGLLARRYGA"
     gene            687..911
                     /locus_tag="VFQ54_02150"
     CDS             687..911
                     /locus_tag="VFQ54_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163814.1"
                     /translation="MDSTQAITEYERLLRLTEEIDGWIEDFIDYQALDPEQEMWRRHW
                     REILDYRDDLTDKIWGIEKQLGIDVEDSRI"
     gene            978..1214
                     /locus_tag="VFQ54_02155"
     CDS             978..1214
                     /locus_tag="VFQ54_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163815.1"
                     /translation="MKIEEPVYCMEESWEWLLRDLRIRRIVHRSRINPDLYQDYHVEH
                     WGPVTDNHYAWTAVNGGLTYATYDEALAVLEAQP"
     gene            1211..1513
                     /locus_tag="VFQ54_02160"
     CDS             1211..1513
                     /locus_tag="VFQ54_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163816.1"
                     /translation="MTLTPEQRERYAKIAYDAHQSWWNSRPETMLAENIPWEEAHEVI
                     TMDYPPMVEAVALAAQRDLIERMKEYYQSGDAETYAIWGDILEWLDSYVPAEAAHD"
BASE COUNT          399 a          501 c          557 g          287 t
ORIGIN      
        1 atggagaacc cgcgcgtcga cgccgtcacg aaggagcgca tgagggtttg gatcacggag
       61 gcggagaccg aactcgtgag ccgcttcggg gaggggatcg gtcactccct cgtcgacgcg
      121 gtccgtagcg gatggaagag cgcgaagacg gagccggcgc tctgggagat gaaggcgggg
      181 attcgggagc cggagacggt ggccgaccag aaccgctatc agtggagcgc ctggtcgtga
      241 ggaggacgta cgagcgagag atcgccaagc tcgaggaacg cgtcgcgtat tggatggaga
      301 tcgcaagact cacaccgtcc gaggacaagc agaatcgcgg gatctgcgtc gcgaacgccc
      361 gcaccgccaa ccgaacgatc gtctacctcc agcgtatcga ccgcctacct gtccagcccc
      421 ctcgttgagg agagttcacg tgatcagtcc acaggtcaag gcacggcgca acatcaactg
      481 cccggccgat ttcctctttc tcgccgcgtc gctctgcatg gagagcgcgc ggaccgcaaa
      541 gcgtatccac atcgacagtc cggccgcgcg gctcgtaatc gcggaacacg tcaagcgagc
      601 acgggcgaac aatcaccgcg gactactcgc tcgccgctac ggcgcctaac gtccgccagc
      661 tgggcggcca ctgaggagag gctgagatgg actcgacaca agcaattacc gaatacgagc
      721 gactgttacg actcaccgaa gagatcgatg gatggattga agattttatc gattaccagg
      781 cactcgatcc ggagcaggag atgtggcgcc gacactggcg cgagattctc gactatcgag
      841 acgatctcac tgacaagata tggggtatcg agaaacaact cggaatcgat gtcgaggaca
      901 gccggatcta acccacccac cagcacacac gggctcacct cgggctcgtg tgtgccgcac
      961 cacgtcagga gcagacagtg aagatcgaag agcctgtgta ttgcatggaa gaatcgtggg
     1021 agtggctgct tcgcgacctg cgcattcgac ggatcgtgca tcgcagtcgg atcaacccgg
     1081 atttgtacca ggattaccac gtggagcatt ggggtcccgt caccgacaac cattacgcgt
     1141 ggacggcggt caatggtggc cttacttacg caacctacga tgaggcgtta gccgtgttgg
     1201 aggcccagcc atgacgctca ccccggagca gcgcgagcgg tacgcgaaga tcgcatatga
     1261 cgcgcatcag tcttggtgga atagtcgtcc cgagacgatg ttagcagaga acatcccgtg
     1321 ggaagaggcg cacgaggtga tcaccatgga ctacccacca atggtcgaag ccgtcgcgct
     1381 cgcggcccag cgggatctga tcgaacgcat gaaggagtac taccagtccg gagatgcgga
     1441 gacgtacgcc atctggggcg acattctaga atggctcgat tcatacgttc cagcggaggc
     1501 tgcacatgac tgaccgcacg gatgaatacg agccctctga cgatccggac tggctcgact
     1561 ggctggcgga tgcgatcgcc gaggagtggg aggacgaagc agcatgaagg atgtcaagtg
     1621 cccgaactgc ggacatgttt tcacacctgg acgtgatccc gaatgggcca aacggatgcg
     1681 cgaccgacga gaaacggcgg ggatttccct gcggtcgctc gcggcaatcc ttggcttcag
     1741 cgcc
//
LOCUS       DASTMD010000138         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2154503, whole genome shotgun sequence.
ACCESSION   DASTMD010000138 DASTMD010000000
VERSION     DASTMD010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2154503"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..2537
                     /gene="pheT"
                     /locus_tag="VFQ54_02165"
     CDS             81..2537
                     /gene="pheT"
                     /locus_tag="VFQ54_02165"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU0163817.1"
                     /translation="MNVPYKWLQEFVPLNIAPRELAHRMTMAGLEAEKIEELGAGWDK
                     VYVGEVTHVERHPDADKLVLADVDAGEHSLRVVTGAPNIAEGQKVALAIAGARLVDAY
                     AEEYKLKTLKPGVIRGVKSEGMVCSEKELGISDEHEGILVLDPAAPKGVPLKEWLGDS
                     VIEFEITPNLVHAFSVLGIAREAGAVTSLPVKAPEVATLAADLETDTLVEVAAPDYCR
                     RYSAIVIDGVQIAPSPDWLAQRLTAAGIRPISNVVDVTNYVMLELGQPLHAFDLSTIS
                     TGKILVRTAAPGEKIETLDHQVRELSADALLITDSGRPVGLAGIMGGVNSEIKDETTS
                     LLLEAANFDMKNIRHTSRALKLRTDASARYERGLDPELCGIANLRATKLILDLCPNAR
                     VRVWQDLYPNPVQPRTVSLPFSRIERVVGIEIPGPEALDALGRLGFAPTLDDATGILT
                     VNVPSWRTDVTIPEDVIEEVARIVGYDRLPATLITGTTPSIERDPTFLLERHIRETLV
                     ASGLFEARTYVTTSDEDIARWSAVETGGLLHAAGESQQVRLRNPIQSDRAVLRTSLIP
                     ALVAATAENLKHERTVRLFEIGHVYIGTEPDQLPNEPSTLGIVFAGYRDAFDRFNARP
                     GEDDQLDYFDVKGAIDAILLRHQHPDLTWDRAEHPALHPGRSAAFALPDGRRLGIIGE
                     VRPDLAAELGIEGVRLIVAEFNTQLLLEQQQDRHAISHLGIERFLPVEQDFAVVVASD
                     VPEAAVEKALLFGAGPLVTGTTLFDVFEGAQLGENRKSLAFRVTFTAPDRALTDAELL
                     KVRKKIERTLAQQVNGTLRA"
     gene            2560..3924
                     /gene="hisD"
                     /locus_tag="VFQ54_02170"
     CDS             2560..3924
                     /gene="hisD"
                     /locus_tag="VFQ54_02170"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEU0163818.1"
                     /translation="MTKEPVSIRIAEGYDEAAAILERRGDFVEPGMAPRVAARTAEVF
                     GAAITAREAVDRIVIAVRDRGDEAVREFTIAFDGAAPDQWEVPREEIEAAWEATSPEL
                     QAALQTSAARIRAFHERQPSVSWIHPEPLGTYGQIVRPLERVGLYAPAGTAPLPSTLL
                     MTAVPARVAGVDEIIVASPPSAGGHVHQIILAAAKVAGVDRVFAIGGAQAIAAMAFGT
                     ESVPHVDKILGPGNLFVALAKQRVYGAVDIDQIAGPTETLVIADESADPMLVAADMIA
                     QAEHDVVASATLLTTSRELAQLVEAQLTIQLADLERGEIARESLRRNGLIAIVESLEA
                     AVSLSNAYAPEHLCLLVANPWDLVPLVRNAGGIFLGEQSAEALGDYTAGPSHVMPTGG
                     TARFSSPVNLRDFQKVISLIGANERALNELGQATSALARAEGLTGHARAIEFRTSVES
                     HRIP"
BASE COUNT          708 a         1396 c         1282 g          669 t
ORIGIN      
        1 cctcagcatc tagtcgttcg cgggcggcca tttcggtcgc ccgccggaac cggggaatca
       61 tcaagggaag acaccacggc attaacgttc catacaaatg gttgcaggaa ttcgttccgc
      121 tcaacatcgc gccgcgcgag cttgcccacc ggatgacgat ggccggactc gaagcggaga
      181 agatcgagga actcggcgcg ggctgggaca aggtctacgt cggcgaagtg acacatgtcg
      241 agcgtcatcc ggacgccgac aaactcgtcc tcgccgatgt cgacgccggt gagcactcgc
      301 tgcgtgtcgt caccggcgcc ccgaacatcg cggagggcca gaaggtcgcc ctcgcgatcg
      361 cgggcgcccg cctcgtcgat gcctacgcgg aagagtacaa gctgaagacg ctcaagcccg
      421 gcgtcatccg cggcgtgaag tcggaaggca tggtctgctc cgagaaggag ctcggcattt
      481 ccgacgagca cgagggcatc ctcgtcctcg atccggcggc gccgaagggg gtcccgctca
      541 aggaatggct cggcgactcg gtgatcgagt tcgagatcac gccgaatctc gtgcacgcct
      601 tctccgtcct cggaatcgcg cgtgaggcgg gtgcggtgac gtcgctccca gtcaaggcac
      661 ccgaggtcgc gacgctcgcg gccgatctcg aaaccgacac cctcgtcgag gtcgccgcgc
      721 cggactactg ccgtcgctac tcggcgatcg tgatcgacgg cgtccagatc gcgccatcgc
      781 cggactggct cgcgcagcga ctgactgccg cgggcatccg cccgatcagc aatgtcgtcg
      841 acgtcacgaa ctacgtgatg ctcgaactcg gccagccgct ccacgccttc gatctctcga
      901 cgatctctac cgggaagatc ctggtccgca cggccgcgcc gggcgagaag atcgagacac
      961 tcgatcatca ggtccgcgag ctctccgcgg atgcactgct gatcacggat agcggccgcc
     1021 cggttggtct cgccgggatc atgggtggcg tcaactccga gatcaaggat gagacgacgt
     1081 cactcctcct cgaagcggcg aacttcgaca tgaagaacat ccgccacacg tcgcgggccc
     1141 tgaagctccg cacggacgca tcggcgcgat acgaacgtgg tctcgatccc gagctctgcg
     1201 gcatcgccaa cctgcgcgcg acgaagctga tcctcgatct ctgcccgaac gccagggtcc
     1261 gtgtctggca ggatctctat ccgaacccgg tccagccgcg aaccgtctcg cttcccttct
     1321 cccggatcga acgcgtcgtc gggatcgaga ttccggggcc ggaggcgctg gatgcgctcg
     1381 gtcgcctcgg cttcgccccg acgctcgacg atgccacggg catcctcacc gtcaatgtcc
     1441 cgtcgtggcg gacggatgtc acgatccccg aggacgtgat cgaggaagtc gcccgcatcg
     1501 tcggctacga tcgcctgccc gccacgctga tcaccggaac cacgccgtcg atcgagcgcg
     1561 atccgacctt cctgctggaa cgccacattc gcgaaacgct cgttgcttcc ggactcttcg
     1621 aggcccggac gtatgtcacg acctcggacg aggacatcgc gcgttggtcg gcggtcgaga
     1681 ccggcgggtt gctccacgct gccggggaga gtcagcaggt ccgtctgcgc aatccgatcc
     1741 agtcagatcg agccgtgctc cggacgtcac tgatcccggc cctcgtcgcc gccaccgccg
     1801 agaacctgaa gcacgagcgc accgtccgtc tgttcgagat cgggcatgtc tatatcggga
     1861 ccgaaccgga tcaactgccg aacgagccct ccacgctcgg tatcgtcttc gccggctacc
     1921 gcgacgcctt cgatcgtttc aacgcgcgac cgggtgagga cgatcaactc gactacttcg
     1981 acgtgaaggg agcgatcgac gcgatccttc tccgccatca gcatcctgac ctgacctggg
     2041 atcgtgccga gcatccggct ctccatcccg gacgctcggc ggctttcgcg ctgccggatg
     2101 gtcgacgact cgggatcatc ggcgaggttc gacccgacct cgccgccgaa ctgggcatcg
     2161 aaggggttcg cctcatcgtg gccgagttca atacgcaact gcttctcgag cagcagcagg
     2221 atcgtcacgc gatcagccat ctcggcatcg agcgattcct gccggtcgaa caggacttcg
     2281 cggtggtcgt tgcgtccgac gtgccggagg ccgcggtcga gaaggccctg ctcttcggcg
     2341 ccggtcccct cgtcactggc acgaccctct tcgatgtctt cgagggcgcc cagctcggcg
     2401 agaacaggaa gagcctcgcc ttccgcgtta ccttcaccgc gcccgaccgc gcgctgaccg
     2461 atgccgagct cctcaaggtc cgcaagaaga tcgaacgaac gctggcccaa caggtgaatg
     2521 ggactctccg cgcatgatgc gcacgatcgg aacaagacga tgacaaagga accggtttcg
     2581 atccggattg ccgaaggcta tgacgaggcg gcagcaatcc tcgaacgcag gggggacttc
     2641 gtcgagccag ggatggcacc ccgcgtcgcc gcacgcaccg ccgaagtctt cggagctgcg
     2701 atcacggcac gcgaagccgt cgatcgcatc gtgatcgcgg tccgcgaccg cggcgacgag
     2761 gcggtccgcg aattcacgat cgcgttcgat ggcgcagcgc cggaccagtg ggaagttccc
     2821 cgcgaggaaa tcgaggcagc ctgggaggcg acatcgccgg aactccaggc tgccctccag
     2881 acatcggcag cccgcatccg ggcgttccac gagcgtcagc cgagcgtttc atggatacat
     2941 cccgagccgc tcggcaccta cgggcagatc gtccgcccgc tcgaacgcgt cggtctctac
     3001 gcgcccgccg gaaccgcacc cttgccgtca acgctcctga tgaccgccgt gcctgcccgc
     3061 gtcgcgggtg tcgacgagat catcgtcgcc tccccgccga gtgcgggcgg gcacgtccac
     3121 cagatcatcc ttgccgcggc gaaggttgcc ggggtcgatc gcgtcttcgc aatcggcggg
     3181 gcccaggcga tcgcggcgat ggcgttcggc accgagtcag tgccacacgt cgacaagatc
     3241 ctcggaccgg ggaatctctt cgtcgcgctg gcgaagcaac gcgtctacgg cgccgtcgat
     3301 atcgaccaga tcgccggtcc gaccgaaacc ctcgtgatcg ccgacgagtc ggcggatccg
     3361 atgctcgtcg ccgccgacat gatcgcccag gccgagcacg atgtcgtcgc gtcggcgacg
     3421 ctgctgacga cgtcgcgcga gctggcccaa ctcgtcgaag cacagctgac gatccagctc
     3481 gccgacctcg aacgcggcga gatcgcgcgc gagtcgcttc gccggaatgg cctgatcgcg
     3541 atcgtggaat cgctggaggc ggcggtctcg ctttcgaacg cctacgcacc ggagcatctc
     3601 tgcctgctgg tggcgaaccc gtgggatctc gtaccgctcg tccgcaacgc ggggggcatc
     3661 ttcctcggcg agcagagcgc cgaggcgctt ggcgactaca ccgctgggcc gagccacgtc
     3721 atgccgactg gcggcacggc gcgtttctcg tcgccggtca atctccggga cttccagaag
     3781 gtgatctcgt tgatcggcgc gaatgagcgc gcattgaacg aactgggaca ggcgacatcg
     3841 gccctcgccc gcgccgaggg tctgacgggc cacgcccgcg ccatcgagtt ccggaccagc
     3901 gtcgagtcgc accgcatccc gtagcgcgcc agctggtctg gcccatcgag cgcagctcga
     3961 tatagtgccg gtgtgctgct cccgcgtccg ctttgcggcg ggggatgaga cccaatgctg
     4021 tcgcataaag gcttgtcatt tcgagcctgt cgaga
//
LOCUS       DASTMD010000139         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2690346, whole genome shotgun sequence.
ACCESSION   DASTMD010000139 DASTMD010000000
VERSION     DASTMD010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2690346"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(761..>2099)
                     /locus_tag="VFQ54_02175"
     CDS             complement(761..>2099)
                     /locus_tag="VFQ54_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEU0163819.1"
                     /translation="KLLAPSDNNPLNLRPWWEDPHPPGGVAGVKQDFLQFDSPVSCVR
                     EWRRRIVDDPSYKKGAYVEAMTLAEMVAIYAPAGDVHPITGTDNADIGYADAVASMLR
                     RFASLEVTESPAKGGQNADDLAPTAFAPPAFLPFLDDASSRELIVREGENVFFRIDAE
                     VEAIRPTPRYARADVASSRLGKDMAIGERASVEWGWIARDGTFWMYSSYATRFKGVDF
                     IPVGASIAVPGPLPGTPTIPEPVPGTITAGNYRLVENPETLLPAIVWRGSTNFFPNRH
                     GRGKAIAIVYHCTDDLVLANTIGWFQNPASKASAHFVIDRDGTVHQFVSSVDAAWTNG
                     DWQVNGDPGYRSDIDWLVDAIEAGDNVNDYTIAYEFVATPETPPTEAQYVAAIGLSRY
                     FCHPRVLGINANRSHQLRHADINRISRGYCPGPGFDLARIIRELGGDPARIAG"
BASE COUNT          371 a          704 c          639 g          385 t
ORIGIN      
        1 aacgagatca ccctgctcgt gcagaaggac gatctcttca tccgtatctc cgccgccagc
       61 gtcggcgccg acccgacgcc cagggccgcg accatcctcc agacgatcct cgccagggcg
      121 tagatccccg tcattcacgc aagaacgccc ggctcgccga tgtggcgagc cgggcgttct
      181 tgcggccaga tcttgatttc gacgcgatca caaatccctt cgcggaatac gtgattttac
      241 gaatactccg tgcggtgacc agatggcaga atcttcatgg gttcaggaaa tgacgcgcga
      301 gcgcatggat gagcagcctg ggaaccaccg ccgggaacgg tgtagccctg ctgtgtccgt
      361 cttctcctgc gacgttccgg agccccccgc cgcacgtcgc aattccgctg gtggtgccgg
      421 ctgcgacgtg cgggcggtgc gggatatccg tctagcagtc tgtcacccgt gtttttccgg
      481 ttggcatcgt aggccaacga tcatatgcgg ctccgccgca tgggccacac ccttcggcga
      541 actcagggca ggctcgttgg tggcccgcta cgggatcacc gtggcaattc agctgtgacg
      601 cacctctagg agtctgcaca ccgaggtgca gctgccggac cgccacgtga cgtgccgcgg
      661 tcgttcgtag ggcgggaccg ccgctgccgg atcgcagatc cggacggcct gtcccgcctc
      721 tcgctcatag cctgccgcct gttcctgccc aatccatcgc ctacccagcg attcgcgcgg
      781 gatcgccgcc aagctccctg atgattcgcg cgaggtcgaa ccccggaccc gggcagtagc
      841 cacggctgat ccggttgata tcggcatgac ggagctgatg cgagcggttc gcgttgatcc
      901 cgagcaccct cggatggcag aagtagcggc tcaggccgat cgccgcgaca tattgcgcct
      961 ccgtcggcgg cgtctccggc gtcgcgacga attcgtacgc gatcgtgtag tcgttgacgt
     1021 tatcccccgc ctcgatcgca tcgaccagcc agtcgatatc ggaccgatat ccggggtcgc
     1081 cattgacctg ccagtcacca ttcgtccagg cggcatccac gctactgacg aactgatgga
     1141 ccgtcccgtc gcgatcgatc acgaaatgcg cggatgcctt gctggccgga ttctggaacc
     1201 aaccgatcgt attcgccagc acaagatcgt ccgtgcagtg gtagacgatc gcgatcgcct
     1261 tcccccgccc atgacgattc gggaagaagt tggtcgatcc cctccagacg atcgccggca
     1321 ggagcgtctc cggattctcg acgaggcggt agttccctgc cgtgatcgtc cctggcactg
     1381 gttccgggat cgtcggggta ccgggcagcg gacccggcac cgcgatcgac gcaccgacag
     1441 gaatgaagtc gactcccttg aaccgggtcg catagctcga gtacatccag aaggtgccgt
     1501 cccgcgcgat ccatccccat tcgacggagg cacgctcgcc gatcgccatg tcctttccga
     1561 gccgcgacga cgcgacatcc gcgcgcgcat atcgtggcgt cggccggatc gcctcgacct
     1621 cggcatcgat ccggaagaag acgttctctc cctcgcgaac gatcagttca cgagaactgg
     1681 cgtcgtcgag aaacggcagg aacgccggtg gcgcaaacgc ggtgggtgca aggtcatcgg
     1741 cgttctgacc acccttcgcc ggcgattcgg ttacctcgag actcgcgaac cggcgcagca
     1801 tcgaagcgac ggcgtccgca tagccgatgt ccgcgttgtc cgtcccggtg atcggatgca
     1861 cgtcacccgc tggtgcgtag atcgcaacca tctcggcgag cgtcattgcc tccacgtaag
     1921 cacccttctt ataggaagga tcatcgacga tccgtcgtcg ccattcgcgc acgcaggaga
     1981 ccggcgaatc gaactgcagg aagtcctgct tcaccccggc gaccccaccc ggcgggtgag
     2041 gatcctccca ccacggccgg agattgagcg gattgttgtc ggagggggcg aggagcttg
//
LOCUS       DASTMD010000140         3059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1262314, whole genome shotgun sequence.
ACCESSION   DASTMD010000140 DASTMD010000000
VERSION     DASTMD010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262314"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="VFQ54_02180"
     CDS             complement(<1..1297)
                     /locus_tag="VFQ54_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0163820.1"
                     /translation="MSKQERPSNFGAIASTTLDRRAFVGKTAAAGVALTAGVAALGAG
                     IAPARAAGGGTLVVDLGSEPENLDIQQGLAATTSLLTQQIFESLLRVKPGTLDVEPWL
                     AESYESSKDGLTWTFKLRQGVKFHDGTDLDADAVKFNYDRQFDTTNEYYKLGQWSNGG
                     YFDFIKSVTVSDASTVVFTLNSTFNKFDFRMAGFSIVSPAGVKKFRETFTENPVGTGP
                     YKFDHWDKGQQVVLAPNDTYWGTAPTLDQLVFKAITEAGSRAAALLSGETQIAVEVTP
                     EIMEQLASNDAFSVVTGPTGSLWFLAMNVKDPHFTDVRVRQAVNYAIDKETICSAVLN
                     GTADVAHGPLSPAFADYNPAVENLYPFDADKAKSLLAEAGWNSDTEVTFYCSIGGSGM
                     LSPEEMATVIQDNLHDVGFKTKIVTMELVSWMGTIRDPKN"
     gene            complement(1398..2546)
                     /locus_tag="VFQ54_02185"
     CDS             complement(1398..2546)
                     /locus_tag="VFQ54_02185"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0163821.1"
                     /translation="MNLDAIPVIDTHAHPFPPQQGAVSWGLLRDAVSESLRAETTDEN
                     DSMLLMRMLVKRLAKLLDCPADRDSVLAARNALAANPGAYHQMLFADANITAVLVDPG
                     FPSKLIEATDFATVIPCPVYEGYRIERFARGTSQQVPFDYDAYDSFTDLVEAFRAQLD
                     AEAAKPGFRFYKSIIAYRTGLKLTNPSEADVLAAWKSRPGYRTPGEKQLRDFLFHETA
                     LKALEHDVAFQVHTGHTSHDQPWPNANPIEMTLFLNQPRMDEVRFVLVHGGYPFNTEA
                     GYITSAYPKVFLDLSLMIPWSSIGVARRIEETLEFAPTTKVMYGSDGIMVPELFWISA
                     HNGRKALGKVLDGLIADEVLDTDEANDIAHDVLHRTAARVYGVALDRA"
BASE COUNT          552 a          894 c         1016 g          597 t
ORIGIN      
        1 cgttcttggg atcgcggatc gtgcccatcc aactgactaa ttccatcgtc acgatcttgg
       61 tcttgaaccc cacgtcgtgg aggttgtcct ggatgaccgt cgccatctct tctggcgaga
      121 gcatgcccga accgccgatc gagcagtaga aggtgacttc ggtgtcggag ttccagccgg
      181 cctcggcgag gagcgacttc gccttgtccg catcgaacgg atagagattc tcgaccgccg
      241 ggttgtagtc ggcgaaggcg ggagagaggg gaccatgcgc gacgtcggcg gtgccgttga
      301 ggacggccga gcagatcgtt tccttgtcga tcgcatagtt cacggcctgt cgcacgcgga
      361 catcggtgaa gtgcgggtcc ttgacgttca tcgcgaggaa ccagagcgag ccggtgggac
      421 cggtcacgac gctgaatgcg tcattcgagg cgagctgctc catgatctcg ggcgtcactt
      481 cgaccgcgat ctgggtctcg ccgctcagca gtgcggcggc gcgggagcct gcctccgtga
      541 tcgccttgaa gacgagctgg tcgagcgtcg gcgccgttcc ccaataggtg tcgttgggag
      601 cgagtacgac ctgctgaccc ttgtcccaat ggtcgaactt gtacggtccg gtcccgaccg
      661 gattctccgt gaaggtctcg cggaatttct tcacacctgc cggcgagacg atcgagaagc
      721 ccgccatccg gaagtcgaac ttgttgaagg tcgagttcag ggtgaagaca accgtcgagg
      781 cgtccgagac cgtgaccgac ttgatgaagt cgaaatagcc accgttggac cactggccga
      841 gcttgtagta ctcgttggtg gtgtcgaact ggcggtcgta gttgaatttc accgcgtcag
      901 cgtcgaggtc cgtcccatcg tggaacttca cgccctggcg cagcttgaag gtccaggtca
      961 ggccatcctt gctgctctcg taggactcgg ccagccacgg ctcgacgtcg agcgtccccg
     1021 gcttgacgcg aaggagcgac tcgaatatct gctgggtgag caggctggtc gtcgccgcaa
     1081 gtccttgttg gatatcgaga ttctccggct cgctgccgag atccacgacg agcgtgccgc
     1141 cacccgctgc ccgggcgggc gcgatccctg cgccgagcgc cgcgacgcct gcggtcaggg
     1201 cgacaccggc ggctgccgtc ttgccgacga atgcgcgacg atcgagggtc gttgatgcga
     1261 ttgcaccgaa gttcgacggg cgttcctgct tggacatgtt cgacctcact cgtgacgaaa
     1321 agcgaatgcc tgtgacctct acggtcgggg tcccggtacg aaagacgccg gcggcgttcc
     1381 tcctgtcgcc tggcctgtca ggcgcgatcc agtgcgacgc cgtagacacg cgccgcggtg
     1441 cgatgcaaga cgtcgtgagc gatatcgttg gcctcgtcgg tgtcgagcac ctcgtccgcg
     1501 atgaggccat ccagcacctt gccgagcgcc ttgcgtccgt tgtgggcgct gatccagaag
     1561 agctccggca ccatgatccc gtccgagccg tacatcacct tcgtcgtcgg cgcgaattcg
     1621 agcgtctcct cgatccggcg cgcgacgccg atcgaggacc acgggatcat cagggagaga
     1681 tcgaggaaga ccttcggata ggcggacgtg atgtatccgg cctccgtgtt gaagggatag
     1741 ccgccatgaa cgagaacgaa gcggacctca tccatccgcg gttggttcag gaagagggtc
     1801 atctcgatcg gattggcgtt cggccacggc tgatcgtgcg aggtgtgtcc cgtgtgaacc
     1861 tggaacgcga catcgtgttc gagcgccttg agcgcggtct catggaagag gaaatcgcgg
     1921 agttgcttct cgcccggcgt ccggtagccg ggacgcgact tccaggcggc gagaacatcc
     1981 gcctcggacg gattggtcag cttcagtccg gtacggtagg cgatgatcga cttgtagaac
     2041 ctgaatcccg gcttcgccgc ctccgcatcg agctgggcgc gaaatgcctc gacgaggtcg
     2101 gtgaaggaat cgtaggcgtc gtagtcgaaa ggcacctgct gggaggtgcc ccgggcgaag
     2161 cgctcgatcc ggtacccctc gtagacggga caagggatca ccgtcgcgaa gtcggtcgct
     2221 tcgatcaact tcgatggaaa gccgggatca acgaggaccg cggtgatatt ggcatccgcg
     2281 aagagcattt gatggtaggc gccaggattc gcggcgagcg cgttccgggc cgcgaggacc
     2341 gagtcgcgat cggccgggca atcgagaagc ttcgccagcc gcttgacgag catccgcatc
     2401 agcagcatcg aatcgttctc gtcggtcgtc tccgcccgca gggactcgct gaccgcgtcg
     2461 cggagcaatc cccacgagac cgccccctgt tggggcggga atgggtgcgc gtgcgtgtcg
     2521 atcaccggaa tcgcgtcgag attcattccc tgtgtgccct tcagtcgtgg ttggctgtcg
     2581 atgaactgat ctgatgccgg ggaggctcgt cgcccgaaac ccggaatgtg tgtgcgacga
     2641 caggcgagtc ttggtcatta cgcacaatgt actaaccgta ttgaacgttt gtcggccaat
     2701 ctgcatggac gtgttacaac tgtgttgcag cataggtggg ggctgataga ctgtcaacgg
     2761 catgtgggcg acgaaattca gaagatttga ccagttcgtc caaccattgc cggcccggat
     2821 gttgcgtttg atacagtcgg gcgaaacggg ttccacaggc aggggcacgc gagtgtgcca
     2881 gctgttacac gacgacgaag ccggcacggc ggtcgcgcca ttcacggaaa cgccgggatc
     2941 cagactcacc ggcgcgcgtg cgtcgatgag cgttgtttgg agtgggtgat cgatgcagaa
     3001 gccgttgcac ctgggactcg ccgaggatct gattgcccgc atcgatcgcg gcgagtgga
//
LOCUS       DASTMD010000141         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1976710, whole genome shotgun sequence.
ACCESSION   DASTMD010000141 DASTMD010000000
VERSION     DASTMD010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1976710"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..818)
                     /locus_tag="VFQ54_02190"
     CDS             complement(159..818)
                     /locus_tag="VFQ54_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU0163822.1"
                     /translation="MSELLPIFPLGTVVFPGMPLPLHIFEERYRRMLADRVEHDPAFG
                     IVLLRTGRDNGTEQTLFSVGTAARIVSRRTRRDGRSDIVIEGTRRFDIEDIRWDLGYG
                     LATIQWRDEMIGNTDHLEATRDIAIEQFQRYVRGVTKITGRRFRGIRLGEDPTSASYD
                     LASRLPLHTWERQDLLEAESVEARFESLLRIVRREVALLAKSGTAGLAINHPGQTFTL
                     N"
     gene            987..2582
                     /locus_tag="VFQ54_02195"
     CDS             987..2582
                     /locus_tag="VFQ54_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0163823.1"
                     /translation="MASGAAAILASASVREVVGQEATPAWSPADGLRLAGPAGGLMTL
                     DPAVARDFQEMSILRQVFRGLLGYSTKLETVPDLAASVTASEDVRVFAVALRPGIAFH
                     DGRAITSRDVHDSFVRALNPATAGGDASGLSAAIYLGGIVGAADVLAGTTTTLAGFAI
                     SSDATFEIHLTGADSAFPQKLASVPAAIVDASTAGTGGVPNGTGPFRVDSFVPNSALA
                     LSASSRWYEGAPEITSLSYRLGAGAGNPLNLYQAGEIDILDGIADDQMELLADPASGA
                     SLGDVTKTNLFSLYYLALGSGQPPLDDVHVRRALQLAFPAAKFAAAGGATVTPARGFI
                     PPGMLGEQWSATLPAMNLDAARSELRKSRYARAADVPPITIYGGQGQGAGPLRNVGVA
                     MRDVVGAELGLTIEPVWIDWNDYLLGLPQGLYPTYSITWVADYPDPESMLWVLFGSDS
                     SENYSGYRNDAFDALLASARAEPDDARRASIYTDAQQVLIDDAVVMPFFFDISYTVAR
                     PGIAGVPASAIGMLGLESVRKST"
BASE COUNT          450 a          889 c          895 g          505 t
ORIGIN      
        1 ctctatgcgg tgcagcggga tcgtctcgcc tcggcttttg ccgaggcgga tgtcgtcatc
       61 gattcggcca ccaacgaccc cgaccaggtc gcggcccgcg tcctcggatg gctgcgccag
      121 cacccgccat cttgccgggg ctcaggtcgg ccgaatctct agttgagggt gaaggtctgg
      181 ccgggatgat tgatcgcgag tccggctgtg cccgacttcg cgagcagcgc gacttcccgc
      241 cggacgatcc tgagcaggga ctcaaagcga gcctccacgc tttcggcctc cagcaggtcc
      301 tgccgctccc atgtatgaag cgggaggcgt gaggcgaggt cgtacgaggc gctggtggga
      361 tcctcgccga gccggatccc gcggaatctg cgtccggtga tcttcgtcac accgcggacg
      421 tagcgctgga attgctcgat cgcgatgtcc cgcgtggctt cgagatggtc agtgttgccg
      481 atcatctcgt cacgccattg aatcgtggca aggccgtagc cgagatccca ccggatgtcc
      541 tcgatatcga aacggcgcgt tccctcgatc acgatatccg agcgaccatc ccggcgcgtg
      601 cggcgggaga cgatgcgtgc cgcggtgccg accgagaaga gcgtctgctc cgtcccgttg
      661 tcccgtcccg ttcgcaggag cacgatcccg aacgccggat cgtgttcgac gcgatcggcg
      721 agcatccgcc ggtaacgctc ctcgaagata tggagcggca gtggcatgcc agggaagacg
      781 acggttccga gtggaaagat gggaagcagt tcggacatgg ggctgatgat cgatccctgt
      841 agaagactgc gaacaaatcg ttcaaccatt ctacggtgcg ctggaagcaa ccacactgcc
      901 catgtggttg cttccagcgg gctgcgtgaa aagcggcaca atcgcagtac acttttctca
      961 tgaagacgac gtcgcgacga caactcatgg cttccggcgc ggcggcgata ttggcgagcg
     1021 catcagtgcg cgaggtcgtt gggcaggagg cgacccccgc ctggtcgcca gccgatggtt
     1081 tgcgcctcgc aggaccggcc ggcggcctga tgacgctgga tcccgccgtt gcgagggact
     1141 tccaggagat gtcgattctc cgccaggtct ttcgcgggct cctcggctat agcacgaagc
     1201 tcgagaccgt ccccgatctc gccgccagcg tgacggcgtc ggaggatgtc cgggtgttcg
     1261 ccgtcgcgct gcgtcccggg atcgcgtttc acgatggtcg tgcgatcacc tcgcgggacg
     1321 tgcatgactc gttcgtccgc gcgttgaacc cggcgaccgc gggtggtgac gcgtccggcc
     1381 tgtcggccgc gatctacctg ggtggaatcg tcggcgcggc cgatgtgctc gcggggacga
     1441 cgacgacgct cgccggattc gcgatctcgt cggacgcgac cttcgagatc cacctcaccg
     1501 gcgccgattc cgcctttccc cagaagcttg cgagtgtccc ggcggcgatc gtcgacgcgt
     1561 cgacggccgg gaccggcggc gtgccaaacg gaaccggtcc gttcagggtg gattccttcg
     1621 tgccgaattc cgcgctggcc ctctcggcga gttcgcgctg gtatgaggga gcgccggaga
     1681 taacctcgct ctcgtatcgt ctcggcgccg gcgcgggcaa ccccctgaac ctctatcagg
     1741 cgggggagat cgatatcctc gacgggattg cggacgacca gatggaactg cttgccgatc
     1801 ccgcgagtgg ggcgtcactc ggcgacgtga cgaagacgaa cctcttctcc ctctactacc
     1861 tcgcgctggg ctcgggtcag cccccgctcg acgacgtgca cgtgcggcga gcgttgcagc
     1921 tcgcttttcc cgccgcgaag ttcgccgccg cgggcggtgc aactgtcacg cccgcgcggg
     1981 ggttcatccc gcccgggatg ctgggcgagc agtggagtgc cacgcttccg gcgatgaacc
     2041 tcgacgccgc gcgctccgaa ctccgcaagt cacgttatgc gcgtgccgcg gacgttcccc
     2101 cgatcacgat ctatggcggc cagggacagg gcgcgggccc gctgcgcaat gtcggcgtcg
     2161 cgatgcggga tgtcgtgggc gcggaactcg ggttgacgat cgagccggtc tggatcgact
     2221 ggaacgacta tctgctcggc ctgccgcaag gactctatcc cacctacagc atcacctggg
     2281 tcgccgatta tcccgatccg gaaagcatgc tgtgggttct ctttggaagc gacagcagcg
     2341 agaattactc agggtatcgt aatgatgcct tcgatgcgct cctcgcgagc gcgcgcgcgg
     2401 agccggatga cgccaggcga gcctccatct acacggacgc ccagcaggtg ctgatcgacg
     2461 atgccgtcgt gatgccattc ttcttcgata tcagctatac ggtcgctcga ccgggaatcg
     2521 ccggcgtgcc ggcatccgcg atcggcatgc tcggactcga atccgtacga aagtcaacat
     2581 gaagcgcgtc tcgctccaca tcccccagtc ccatcgccgc caggagatcg atattcgtcc
     2641 gttaacccca gcggacgtcg aacgcgtgat catcgatcca cgtcgtggca tcgacgctga
     2701 cgagttgatg gcgctcgtcg tccaggcgcc cggcatgtc
//
LOCUS       DASTMD010000142         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_816925, whole genome shotgun sequence.
ACCESSION   DASTMD010000142 DASTMD010000000
VERSION     DASTMD010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816925"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="VFQ54_02200"
     CDS             complement(<1..1224)
                     /locus_tag="VFQ54_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0163824.1"
                     /translation="MIVQVNARHDPDTSVDRWRAGRKQKRRRMRTEKRGMVAVEQFDV
                     AIIGAGQAGGPLGSAFGNAGRKTIVIERIHVGGTCVNEGCTPTKTMIASGDIAYRARR
                     GADFGVGTGEITISMEKIRDRKRTNVRIWREGSENGLTSTPNVELLMGEASFTGPKTL
                     RVAANDGTVREIAAGTIVINAGERPAPPRVEGAEHVSYLTSTTIMELDVVPEHLVVIG
                     GGYVGLEFAQLFRRLGSDVTVVQHGNQLLSREDPDVAEALHDILVDDGLTIYLNAQTT
                     HVSGADGAIELTIAVGGETVDIRGSHLLAAAGRIPNSDSLALDAAGIETDARGYIVTD
                     DRLETNVPGVYAVGDIRPGPKFTHISYDDYRILQANLIDGGERSILDRQEPYTVFTEP
                     QLGRIGLSEEDAKRQG"
     gene            1252..1749
                     /locus_tag="VFQ54_02205"
     CDS             1252..1749
                     /locus_tag="VFQ54_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0163825.1"
                     /translation="MHCQQCGGTTETQRMADGHERPVCSACGAVTYLDPKLAVTVVIE
                     RDGAILLGKRGEPTRNAGLWSFPAGFIDRGEAVEAAAIREVGEETGLTIELGPILDVY
                     STAGDSTVLLVYPATSITGEATAQDDLLELGWFPPSEFPNLPLAFAHDLDILATWQSW
                     RAARA"
     gene            1911..>2640
                     /gene="trpS"
                     /locus_tag="VFQ54_02210"
     CDS             1911..>2640
                     /gene="trpS"
                     /locus_tag="VFQ54_02210"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU0163826.1"
                     /translation="MPKDQRLLTGDRPTGALHLGHLVGTLRNRVRLQHDYECYFIIAD
                     LHMLTTKSEPEHIAAVDTNARGLVLDAISAGLEPESVTFYLQSAIPEIHELAGLLQSL
                     VIVSRLERLPALKEMARDANQEMSLALLGYPVLQSADIFSVRAHAVPVGKDNYPFVEI
                     SREIARRFNARYGEVFPVPQIVPGEVPTLIGTDGQRKMSKSLGNSIELRDSAEDVRRK
                     VMRMYTDPNRLTADTPGTVEGNPVC"
BASE COUNT          473 a          914 c          779 g          474 t
ORIGIN      
        1 gccctgtcgt ttcgcatcct cttcggagag cccgatccgg cccaactggg gctcggtgaa
       61 gaccgtgtac ggctcctggc gatcgagaat cgaacgctcg ccaccgtcga tcaggttcgc
      121 ctgaaggatc cggtagtcat cgtaggagat gtgcgtgaac ttcggccccg ggcggatatc
      181 accgacggcg tagacaccgg gcacgttcgt ctcgaggcga tcgtcggtga caatgtatcc
      241 acgggcgtcg gtttcgatcc ccgctgcatc aagcgccagc gaatcggaat tcgggatacg
      301 tcccgccgcc gccaggaggt gggatccgcg gatgtcgacc gtttcgccac cgaccgcgat
      361 cgtgagctcg atcgcgccat cggctccgct tacatgcgtc gtctgcgcgt tgaggtagat
      421 cgtgaggccg tcgtcgacaa ggatgtcgtg caacgcctcg gcgacatccg ggtcttcgcg
      481 cgagagcagc tggttgccat gctggacgac cgtcacgtcg gaaccgagcc ggcggaagag
      541 ctgcgcgaac tcgaggccga cgtagccgcc accgatcacc acgaggtgct ccggcaccac
      601 atcgagctcc atgatcgtgg tcgacgtcag gtaggagacg tgctcggcac cctcgacgcg
      661 cggtggcgcc ggacgttccc cggcgttgat cacgatcgtc ccggccgcga tctcgcgcac
      721 ggttccgtca ttcgcggcga cgcgaagcgt cttcggcccg gtgaagctcg cctcgcccat
      781 caggagctcg acgttcggcg tgctcgtgag accgttctcg ctgccctcac gccagatccg
      841 gacgttcgtc cgcttgcgat cgcggatctt ctccatcgag atcgtgatct cgcccgtgcc
      901 gacgccgaag tccgcaccgc gacgcgcgcg ataggcgatg tcgccgctgg cgatcatcgt
      961 cttggtcggt gtgcatccct cattgacgca tgtgccgcca acgtggatcc gttcgatcac
     1021 gatcgtcttg cgaccggcat tgccaaacgc ggatcccaag ggtccgccgg cctgccccgc
     1081 gccaatgatt gcaacatcaa actgctccac agccaccata cccctctttt ctgttcgcat
     1141 tcgtctgcgt ttttgcttgc gaccggcacg ccatctgtct acgcttgtat ctggatcgtg
     1201 gcgagcgttt acctgcacga tcatatatga cagcggatgg acgagtcggc aatgcactgc
     1261 caacaatgcg gcgggacaac cgaaacacaa cgcatggcgg acggacacga acgtcccgtc
     1321 tgttcggcgt gtggcgccgt cacctacctc gatcccaagc tggcagtgac cgtggtgatc
     1381 gaacgcgacg gcgcgatcct gctcggcaag cggggcgaac cgacgcgtaa cgccggcctc
     1441 tggtcgtttc ccgccgggtt catcgatcgc ggcgaagccg tggaagcggc cgccattcgt
     1501 gaagtcggcg aagagaccgg cctgacgatc gaactcggac cgatcctcga tgtctactcg
     1561 acggcaggcg attcgaccgt gctcctcgtc tacccggcga catccatcac cggcgaggcg
     1621 acggcgcaag acgatctcct cgagctcggc tggttcccgc cctccgagtt cccgaacctc
     1681 ccgctcgcct tcgcgcacga tctcgacatc ctcgcgacct ggcagagctg gcgcgccgcg
     1741 cgcgcctgat acctacccca ccaggggaga ttctccccac cccatcttgc gaatccgata
     1801 cgtgacaccg tcgcggcggg acaccgcgtc cagaatcgcc gttccgcggc gcaagatgga
     1861 cgtccacccg actccacggt ctcgcacacg atgctgaaag ttgaaccaag atgccaaaag
     1921 accaacggct gctcaccggc gaccgtccga ccggggccct tcatctcggc catctcgtgg
     1981 gaacgctgcg caatcgcgtc cgcctgcagc atgactacga gtgttatttc atcatcgccg
     2041 acctccacat gctcacgacg aagagcgagc ccgaacacat cgccgccgtc gatacgaatg
     2101 cacgcgggct cgtgctcgac gcgatcagcg cgggtctcga accggaatcc gtcaccttct
     2161 acctgcaatc ggcaattccg gaaatccatg aactggccgg tctcctgcag agcctcgtca
     2221 ttgtcagtcg actcgagcgc cttcccgccc tcaaggagat ggcgcgcgac gccaaccagg
     2281 agatgtccct cgccctgctc ggttacccgg tgctgcaatc ggcggacatc ttctccgtcc
     2341 gcgcccacgc cgtgccggtc gggaaggaca actacccctt cgtcgaaatc tcgcgcgaaa
     2401 tcgcccgccg cttcaacgcg cgctacggcg aggtcttccc ggtgccgcag atcgtccccg
     2461 gcgaagtccc gacgctgatc ggtaccgacg gccagcgcaa gatgagcaag agtctcggca
     2521 attcgatcga actccgggac tccgccgagg atgtccgtcg caaggtgatg cggatgtata
     2581 ccgatcccaa ccgcctcacg gcggacacac ccggcacggt cgaggggaat cccgtatgca
//
LOCUS       DASTMD010000143         1760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_102937, whole genome shotgun sequence.
ACCESSION   DASTMD010000143 DASTMD010000000
VERSION     DASTMD010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102937"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VFQ54_02215"
     CDS             complement(<1..1239)
                     /locus_tag="VFQ54_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0163827.1"
                     /translation="MPLWRDPRRLKTNLVSIGRGARETWRAVRQVLALVWATSRILTS
                     GLAATSLFQSVLPAIQVWLAGRLIDDVVSGFNAGGDDSHIRGVIILASIQLGILVASS
                     LMQSIGNICQQLLQERLTIHVQLEIMDLAKSLDLADFEDATYYDQLQQAQTESSRRPV
                     QMVQQVFGLMQSLITFSTMVALLTSLSWWIAVAALASPIPAFISQSKYGWQGFHMMRR
                     QSPLRRSMSYLTMVMTTDQYAKEVKLFTIGDYFITRYKRIAASYYDEVRSLLMRRYLS
                     SFGWGSITTIASSGTFLYVAVLAIRGQISLGSLTVYTQAANQVQNAFQGILGGFQGIY
                     ENGLYLQTYYDLLEKKPRIVAPEHPAPIHVPFRQGVEFRDVSYRYAGREEWALRHLSF
                     TIEPGETVALVGRNGAGKSTI"
BASE COUNT          340 a          514 c          559 g          347 t
ORIGIN      
        1 gatcgtcgac ttgccggcgc cgttgcgtcc gaccagtgcg accgtctctc ccggctcgat
       61 cgtgaacgag aggtgcctga gcgcccactc ctcacgtccc gcatagcgat agctgacatc
      121 gcggaactcg accccctgcc ggaacggcac gtggatcggc gccggatgct ccggtgcgac
      181 gatgcgcggc ttcttttcga gcagatcgta gtaggtctgc aggtacagcc cgttctcgta
      241 gattccctgg aatccaccga ggatcccctg gaaggcgttc tgcacctggt tcgcggcctg
      301 ggtgtagacc gtcagcgagc cgagcgagat ctgtccccgg atcgcgagga cggcgacgta
      361 gaggaacgtc ccggaagagg cgatcgtggt gatcgatccc cacccgaacg aggagaggta
      421 ccgccgcatc aggagcgagc ggacttcgtc gtaataactc gcggcgatgc gcttgtagcg
      481 cgtgatgaag tagtcaccga tcgtgaagag cttgacttcc ttcgcgtact gatcggtcgt
      541 catcaccatc gtcaggtagc tcatcgagcg ccggagcggc gactggcgcc gcatcatgtg
      601 gaagccctgc cagccgtact tcgactgcga gatgaacgcc gggatcggac tggcgagcgc
      661 ggcgaccgcg atccaccagg agagactggt gagcaacgcg accatcgtgc tgaaggtgat
      721 cagcgattgc atcaatccga agacctgctg gaccatctgg accggacgtc gtgacgattc
      781 cgtctgcgcc tgctgaagct ggtcgtagta ggtcgcatcc tcgaaatcgg ccagatccaa
      841 cgatttcgcg agatccatga tctccagctg gacgtggatc gtgaggcgct cctggagcag
      901 ctgctgacag atgttgccaa tcgactgcat caggctcgag gcaacgagga tgcccaactg
      961 gatcgacgcg aggatgatca cgccacggat atgggagtcg tcgccaccgg cgttgaagcc
     1021 gctgacgaca tcgtcgatca atcgacccgc cagccagacc tggatcgcgg gaagcacgct
     1081 ctggaagagg ctggtggcgg cgagaccgga ggtgaggata cgactcgtcg cccagacgag
     1141 ggcgaggacc tgacgcacgg cgcgccaggt ttcacgggca ccgcgtccga tcgagacgag
     1201 attcgttttc agccgtcggg gatcgcgcca gaggggcaca gaagaatctc cccgtcggga
     1261 tcctggatcc ggtggggagt ctggtgtcat gggggggact tcgatgggcc gtttgttgtt
     1321 tctgtcgctg ttcgaacggg gacgtggccc gaggttgccg ttcggcgatg ttggatgttc
     1381 atccatgcag taagcatctc tccttccagg gaggttcgcg atcttcatca tcgcgttcaa
     1441 atctttgtta cgtctataga accataaata ggatgtcgct acaactcccc atctgtcgag
     1501 tcatcagcgc catactggac gaaaggttag tcgaattccc gtgatacccg actttcctga
     1561 ggaatttacc ttccgatggg tctcgatgcc ccgtcaatcc gctgtaacac gcgggagaca
     1621 aggcctggtc aagggctacg gaacttcgta ggagagaacg ggttgctcgc cgcggtcctc
     1681 tgaccatcgc gtcaccctcg ttcgtagggg tgcgtcgcgg tcctttgccc atcacgtcgc
     1741 cgtcgtccgt aggggcgaac
//
LOCUS       DASTMD010000144         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2691075, whole genome shotgun sequence.
ACCESSION   DASTMD010000144 DASTMD010000000
VERSION     DASTMD010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2691075"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1379
                     /locus_tag="VFQ54_02220"
     CDS             282..1379
                     /locus_tag="VFQ54_02220"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU0163828.1"
                     /translation="MSAEITVLDLFAGAGGLTAGLHKASDRFRTVGAVEWDQAAAASY
                     EATYGKGIVYAGDIKTWLDEETVPQADLLIGGPPCQGFSTLGKQDVEDERNFLWKEYA
                     RTIRKAMPKYFVVENVAEFKKSPQYRQFKAETSDGGTLPEYDFEATVLNAADFGAPQS
                     RRRTVIIGWHRDLGFPGFPQVTHSANPVRDGLKAHVTVGDALKRVPRSPDRDDVFDSK
                     RTRFAGHEFAGAFRARDLHWSRNYTDLSLRRFAVIPAGGNRRHLEPYPDLMPRCWVDH
                     KTGSGDVMGRLHWDRPSVTIRTEFFKPEKGRYLHPEEDRAITHYEAALLQGFGPKHRF
                     VGSRTDIARQIGNAVPIPLGTAIGRLLAGVL"
     gene            1480..>1629
                     /locus_tag="VFQ54_02225"
     CDS             1480..>1629
                     /locus_tag="VFQ54_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine zipper domain-containing protein"
                     /protein_id="HEU0163829.1"
                     /translation="MSHSKARLAPAGRLILVQRVLSGRPVSHVAKEMGVSRQCAHRWV
                     SRFRSF"
BASE COUNT          283 a          501 c          548 g          297 t
ORIGIN      
        1 ccgcgccgag gatcggcgga tcgacattct gaacgagcga gacctgctca tggaaatgcg
       61 cggcatacgc tgctgcggcc gcatcgccga tcaccggtgc gaacctgctc accgcggctt
      121 cggacgtgta cttgctcgcc cagccggcga agaccggata gaaatccatg tcaacgccat
      181 caccccctgc gctcatgctg gaaacgatat cggaggcccc ggacgtcgag tcggccggcg
      241 tgccggctta ggacgtcggt gccccgaaat agactctccg catgtccgcc gagatcactg
      301 tgctcgacct gttcgcaggc gctggcgggc tgacagcagg cctgcacaag gcatccgacc
      361 gtttccggac ggtcggggcg gtcgagtggg accaggctgc ggcggcgtct tacgaggcca
      421 cctatggcaa ggggatcgtc tatgcgggcg atatcaagac ttggctcgat gaggagaccg
      481 tgccgcaggc cgacctcctg atcggaggac cgccgtgtca ggggttctcg actctcggca
      541 agcaggacgt ggaggacgag cgcaactttc tttggaagga gtacgcgcgg acgatccgta
      601 aggcgatgcc gaaatacttc gtggtcgaga acgtggcgga gttcaagaag tcaccgcagt
      661 accgtcaatt caaagcggag acctctgacg gcgggacgct ccccgagtat gacttcgagg
      721 cgacggttct caacgcggcg gatttcggtg cgccgcagtc gcgtcggcgc acggtgatca
      781 tcggctggca tcgtgacctc gggtttccgg ggttcccgca ggtgacgcat tccgcgaatc
      841 ctgttcggga cggcctgaag gcgcacgtca cggtggggga cgcgctgaag cgagttcctc
      901 gctcaccgga tcgcgacgat gtcttcgact cgaagcgcac gcggttcgcc ggccacgagt
      961 tcgccggggc attccgggcg cgcgatctcc actggagtcg caactacacc gatctttcgc
     1021 ttcgtcgctt cgccgtgatc ccggcgggag gcaaccggcg gcacctcgag ccgtatccgg
     1081 acctcatgcc ccggtgctgg gtggatcaca agaccggttc cggcgacgtg atgggcagac
     1141 tccactggga ccgtccgtcc gtcacgatcc gcacggagtt cttcaagccc gaaaagggaa
     1201 ggtatctcca ccctgaggag gatcgggcga tcacccacta cgaggcagcg ttgctccagg
     1261 gcttcggacc gaagcatcgg ttcgtgggct ctcgcacaga catcgcgcgg cagatcggca
     1321 acgcggtccc gattcctctc gggacggcga tcgggcgcct gctcgctggc gtgctttgag
     1381 cgcctagctg tactcggtca gatcgttggt gacacggaag tgaggagaag gccttcattc
     1441 tgagtggtgt ctagatcact cgaagaggaa ggccttctct tgtcccacag taaagcgcgg
     1501 ctggctccgg ctggtcgcct gatcctggtg cagcgggtgc tgtccgggcg tccggtgtcg
     1561 catgtcgcga aggagatggg cgtctcgcgg cagtgcgcac accgctgggt gtcccggttc
     1621 cggagcttc
//
LOCUS       DASTMD010000145         1515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1621212, whole genome shotgun sequence.
ACCESSION   DASTMD010000145 DASTMD010000000
VERSION     DASTMD010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621212"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..1180
                     /locus_tag="VFQ54_02230"
     CDS             377..1180
                     /locus_tag="VFQ54_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU0163830.1"
                     /translation="MITSRNHPQIRAFRRLLTRKGRQDSGSFIAEGIRQVTTLIESPY
                     VVETLIVAPDLLASATAWDVVARHRSGGGTCLMVSADVFASISVRENPQGLAAVASSG
                     IRRLDEVDASTRRLWVALIDPQDPGNVGTIARTCDAVGAAGIILIGNAVDPYHPRSIR
                     ASVGAVFTQEIVTCSGGDLVAWSRANELPLVGTSDTADLDYRQLPSTREMVLVMGSER
                     QGIPDAMRTHLDSVVRIPMSGSCDSLNLAVATSVILYEIAYGKARREAV"
     gene            1271..>1515
                     /locus_tag="VFQ54_02235"
     CDS             1271..>1515
                     /locus_tag="VFQ54_02235"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEU0163831.1"
                     /translation="MIALISDNLDKIAELCREYRIRKLDLFGSAATGAFDPETSDVDF
                     VVDLGEYERGLAKRLYAFADALESLLGRDVDLVMEDA"
BASE COUNT          267 a          481 c          469 g          298 t
ORIGIN      
        1 cgccgtgagt ccaactgcct gatctgtaac cttggagggg gagcgggacg gtatacgaaa
       61 cgcgtcgtgc ccgatatact cgggatgagt tcctctgtcc ggcttcgtag atggtgtgaa
      121 atgattcgat tgctctgaac ccacgcttcg ccgattcatc ggcaagcatc ctcggcctct
      181 cgtgaggtgt cgggttggtt tgagcatcgc catcgaatat gaagcacgtg tgatacatcg
      241 ctgcgccgca gatgcgtggc ggtcttcacg gccttcctct cgaaccacaa ctccaccctc
      301 aacgtgaatc ctgggatgcg tccgctccga agcgataggt tcttgtcgcc tcggtcacac
      361 gcaacaggac actcttgtga tcaccagtcg caatcacccg cagatccgcg cctttcgtcg
      421 cctcctaacg cggaagggcc gacaggattc cggatcgttc atcgccgaag gaattcgcca
      481 ggtcacgacg ctcatcgaga gcccgtacgt tgtcgagacg ctcatcgttg cgcccgatct
      541 cctcgcgagc gcgacggcct gggatgtcgt cgcccgccat cggtccggcg gtggcacctg
      601 cctcatggtc tcggccgatg tcttcgcgag tatctcggtt cgcgagaacc cgcagggcct
      661 cgccgccgtc gcgtcctccg ggatcaggcg gctcgatgaa gtcgatgcgt cgacccggcg
      721 gctctgggtc gcgctgatcg atccccagga cccgggaaat gtcggcacga tcgcacgaac
      781 gtgcgacgcc gttggcgcgg cagggatcat cctgatcggc aacgccgtcg atccctacca
      841 cccgcggtca attcgcgcct cggtcggcgc ggtcttcacc caggagatcg tcacctgttc
      901 cggcggcgac ctcgtcgcct ggtcacgcgc gaacgaactt ccgctcgtcg gaacctcgga
      961 caccgccgac ctcgactacc ggcagcttcc gtcgacgcgg gagatggtgc tcgtgatggg
     1021 aagcgagcgt cagggcattc cggacgcgat gcgaacgcat ctcgacagcg tggtccggat
     1081 cccgatgagc ggatcgtgtg attcgctcaa cctcgcggtc gcgacctccg tgatcctcta
     1141 cgagatcgcc tatgggaagg cccgccggga agcggtgtaa ggcggaaggt ctgaccctga
     1201 actgcgtcgt cgcgtcgtgc tgggcgctac aatcgtctca ggattgctcg acggcacagg
     1261 gacgacacgc atgatcgcac tcatctccga caacctggac aagatcgcgg aactctgccg
     1321 ggagtaccgg attcgcaagc tcgacctctt cgggtcagcg gccaccggtg ccttcgatcc
     1381 ggagacgagc gatgtcgact tcgtcgtgga tctcggagag tacgaacgag ggctggccaa
     1441 gagactctat gcctttgccg acgcgctgga gtcactgctt ggcagggatg tcgacctggt
     1501 tatggaggat gcgat
//
LOCUS       DASTMD010000146         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1888126, whole genome shotgun sequence.
ACCESSION   DASTMD010000146 DASTMD010000000
VERSION     DASTMD010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888126"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFQ54_02240"
     CDS             complement(<1..839)
                     /locus_tag="VFQ54_02240"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0163832.1"
                     /translation="MASDQSSNGRDGESFDTRFNSKTLYDGPSRAGARSMMKSIGFTD
                     EDLQKPIIGIANTWIETMPCNYNLRRLAKHVKEGVRAAGGTPMEFNTVAISDGVTMGT
                     EGMKTSLVSREVIADSVELVGRGHMFDAIIALGACDKTLPGLSMALARLNVPSFILYG
                     GTIMPGYWQGKEVTVQSVYEAIGAHAAGKMTLEDLKSLEDVACPGAGACAGQFTANTM
                     AMAMEFIGLSPLGTASAPAIDPRKDEIGLKAGELIMDLLKRNVRPLDVLTKASFENAI
                     AGVA"
     gene            1152..2048
                     /locus_tag="VFQ54_02245"
     CDS             1152..2048
                     /locus_tag="VFQ54_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0163833.1"
                     /translation="MSDTVPSIPLSFGLQTVQTWTWPETKERWQWFESLGYQSLWLPD
                     HFVPTASPEGPMFEAWTLLSALATFTERVRIGVLVSSNTFRHPAMLAKQAVTVDHVSN
                     GRLELGVGAGWYVNEHQMFGLDFPSTGELVDRYAEALDLLDRYLGNDITSFEGEYYTL
                     RDAYNRPAAIQSPRPPLMIGAHGPRMIRLAAKYADTWNSRGTPEEMRERNQLMDRALL
                     ANGRQPTDVKRSMLFVPPMMPNEHPWDSIDAFTDFVGRFSEAGVHDFILQTPAVEDFA
                     IVERVAVDVLPGLRARSASFAD"
     gene            2260..2511
                     /locus_tag="VFQ54_02250"
     CDS             2260..2511
                     /locus_tag="VFQ54_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU0163834.1"
                     /translation="MGIVNIVLWILNILSLLILVRALMSWFDPSFRSSIGRAIFDITE
                     PVLAPLRRVIPPMGAIDISTIVAIVLIQILGRLIASSGA"
BASE COUNT          486 a          879 c          851 g          533 t
ORIGIN      
        1 gcaacaccgg cgatcgcgtt ctcgaaggag gccttcgtca ggacatcgag cggccgcacg
       61 ttccgcttga ggagatccat gatcaactcg ccggccttga ggccgatctc gtccttgcgg
      121 ggatcgatcg ccggcgcgga ggcggtgccg agcggcgaga gtccgatgaa ctccatcgcc
      181 atcgccatcg tgttcgcggt gaactggccg gcgcaagcgc cggcgcccgg gcaggcgaca
      241 tcctcgagcg acttcagatc ctccagcgtc atcttgccgg cggcgtgggc gccgatcgcc
      301 tcgtagacgg actggaccgt cacttccttg ccctgccagt agcccggcat gatcgtgcca
      361 ccgtagagga tgaagctggg gacgttgagg cgggcgagcg ccatcgagag acccggcagc
      421 gtcttgtcgc aggcgccgag cgcgatgatc gcgtcgaaca tgtgaccgcg gccgacgagc
      481 tcgaccgaat ccgcgatcac ctcacgcgag acgagggagg tcttcatgcc ctcggtaccc
      541 atcgtcacgc cgtcggagat ggcgacggtg ttgaactcca tcggcgtgcc gccggcggcc
      601 cggacacctt ccttgacgtg cttcgcgagc cggcgcaggt tgtagttgca cggcatcgtc
      661 tcgatccacg tattggcgat gccgatgatc ggcttctgca gatcctcgtc cgtgaatccg
      721 atgctcttca tcatcgaacg cgcgccggcc cggctcgggc cgtcgtaaag cgtcttgctg
      781 ttgaagcgcg tatcgaacga ttcgccgtcg cgtccattgg acgactgatc ggaagccata
      841 tatcctccct gaaaatgtga acctgcatcg ggctggcgtg aaattcgcga acgactgtcc
      901 ttgtctgtcc ggctgaccac ggcgcctggt gccatgacgt catacgtccc ggtcgcggca
      961 aggatacacg cgctatccgg cccgtcgttc gtcacttcga tgggcattct gcacgaggtc
     1021 atctcatcgc acggcaagtt tcacgctcct gcgaagaatt ggcaagtacg cacctacgtc
     1081 gcacaatgga ggatgttcga agcatctccg ggtccctgac tatccgtcgc ctgagaagaa
     1141 tgagagcccc aatgtcggac accgtccctt ccattcccct ttcgtttggc ctccagaccg
     1201 ttcagacctg gacgtggccc gagacgaagg aacgttggca atggttcgaa tcgctcggtt
     1261 accagagtct ctggctgcca gaccacttcg tccccacggc cagcccggaa ggaccgatgt
     1321 tcgaggcctg gacgctcctg agcgccctgg ccacgttcac ggagcgtgtc cgcatcggtg
     1381 tcctcgtcag cagcaacacc ttccgccatc ccgcgatgct ggcgaagcag gccgtcaccg
     1441 tcgaccacgt ctcgaacgga cggctcgagc tcggtgtcgg cgcgggctgg tatgtcaacg
     1501 agcatcagat gttcggactc gatttcccgt cgacgggcga gctggtcgat cgatacgccg
     1561 aggcgctgga tcttctcgac cgctatctcg gcaacgacat cacgagtttc gaaggggagt
     1621 actacacgct gcgtgacgcc tacaaccggc ccgccgcgat ccagtcgccc cgtccgccgc
     1681 tgatgatcgg cgcccacggt ccacggatga tccgtctcgc cgcgaagtac gccgacacct
     1741 ggaactcccg cggcacgccg gaggagatgc gcgagcgaaa ccagctcatg gaccgcgcgc
     1801 tcctcgccaa tggccgccag ccgaccgatg tgaagcgttc gatgctcttc gtaccgccga
     1861 tgatgccgaa cgagcacccg tgggattcga tcgacgcctt caccgatttc gtcggtcgtt
     1921 tctcggaggc gggcgtccac gatttcatcc tgcagacgcc ggccgtcgag gacttcgcga
     1981 tcgtcgagcg cgtcgcggtg gacgtcctcc ccgggctgcg tgcccgcagc gcctccttcg
     2041 cggactagcc cggagccagc ccgcggggcg atcgtcacgc gaatgtgacg atattgcggc
     2101 gaaaccggcg gggaagatgg gttcgcgcat gaacgcatcg ccgcaggaga agatgcggcg
     2161 ttcgatcgtg ctcaccacct ataatcagaa ggcgtttccg atgttgttcg gcgccgcgga
     2221 ccgtgacgta acggtcttga cgtcggaagg tttgcccaga tgggtatcgt caatatcgtt
     2281 ctctggattc tcaacattct cagcctgctc attctcgtgc gggcgctgat gtcctggttc
     2341 gacccatcat ttcgatcgag catcgggcgg gcgatcttcg acatcaccga accggtcctg
     2401 gcaccgctcc ggcgggtgat cccgccgatg ggcgccatcg acatctcgac gatcgtcgcg
     2461 atcgtgctga ttcagatcct ggggcgcctg atcgcgagtt cgggcgcctg atcggacgcc
     2521 ggaatcgtga tgaactggtc acatctctgg acctgtcgcg aagaatcccg taaagtcagc
     2581 gccagaacgc gatctggtcg cgagatgaac tgcacatggt gaaggggcgg atcgaacgga
     2641 ccgtgaggtc cgggacgaga tccgggttag cttggggagc gaagtcatgt tcttgcgact
     2701 gtttctgctg ttgtcgatca tcggccatgt cggcccaagc aactactac
//
LOCUS       DASTMD010000147         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_727819, whole genome shotgun sequence.
ACCESSION   DASTMD010000147 DASTMD010000000
VERSION     DASTMD010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727819"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFQ54_02255"
     CDS             complement(<1..275)
                     /locus_tag="VFQ54_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163835.1"
                     /translation="MNPPAAIGISRSTTDGIPWIENDIDVHDRSDSHPGNSPSRAITS
                     HPRRNTSLVLRRIFRRRVEPDVQIEKGLEKTRKGVFGEITKLFDRTD"
     gene            472..1677
                     /locus_tag="VFQ54_02260"
     CDS             472..1677
                     /locus_tag="VFQ54_02260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0163836.1"
                     /translation="MQRRELILAVYLPTALLAVGQGVLLTTLPFLAESLGVGLTAISL
                     IASAAAIGTLVTDVPAGALLHKIGLRRSMTIGATMVIIGTLSLVLPLNPVAVTSLRVL
                     AGIGTALWELSRHSFITQAVPPAERGRAISTFGGLNRIGMFGGPALGGLIATVFGVHI
                     SFLITGIMAILALASAMIFMSPAHLQQMHYAGSARNRWTMVGDTLRNNAGDMAAASIA
                     QLFAQMIRQGRQLLIPLYGATELGLNPAHVGLVMTISSMVDMSMFFPAGIIMDRYGRK
                     FASVPSFAIMAVGIGIVPFASSFFELILVGILIGLGNGLGSGAMMTLGADLAPPKATG
                     EFLGIWRLIGDVGSVVGPLTVGVVGEWLGLHGSSIFLMFAGLCSSVILLKLVKETRQI
                     KQPEPVSVS"
     gene            1718..>2423
                     /locus_tag="VFQ54_02265"
     CDS             1718..>2423
                     /locus_tag="VFQ54_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-opening dioxygenase subunit LigB"
                     /protein_id="HEU0163837.1"
                     /translation="MPLVAAQIAPHGWDIVDGFTEDAGGALTTRASMKQVGKLFHDAG
                     VEVIVLVGPHGTRVEGAMALTNVARGAGTMIRQGLTWETNVPVDRQFVLEIDRRAQAA
                     GVNTALVGYAGNRADQSAIVMDWGAIVPLTFNGHPENRVGFGTVLGGVPEDDHGPQVV
                     LITPSRAMGRQAMVDFGRVLGEAFRDDPRKIGFIASCDWGHAHTADGPYGFHPDAAKV
                     DAKVVDAIEKNELLSLI"
BASE COUNT          401 a          773 c          753 g          496 t
ORIGIN      
        1 atatcagtgc ggtcgaagag tttcgtgatc tccccgaaga cgccttttcg cgtcttctcg
       61 agaccctttt caatttggac gtcgggttcg actcgacgcc ggaagattct gcgtagaacc
      121 aaggaagtgt tcctccgtgg atgagaggtg atcgctcggg aaggcgagtt gccgggatga
      181 gaatcggagc gatcgtgaac gtcgatgtcg ttttcaatcc agggaatgcc atctgtcgtc
      241 gatcgggaaa tgccgatggc ggctggcgga ttcactccgg aagtatactc cagtggcccc
      301 cttgccctac acggcgcgtg tcaaccctga ccgctttcga ctcccgcgac gccggacaaa
      361 gtgacgcgtc caggataaat atcggtgcag gggtccttcg caggtcggga atcctctgat
      421 aggatcgcca aggactggtc agtcacttgc tcttctcatc cagggacctc gatgcaacgc
      481 cgtgaactca tcctcgccgt ctatttgcct accgcattgc tggcggtcgg gcagggcgtc
      541 ctgctgacga ccttgccgtt cctcgccgag tcactcggcg ttggcctgac ggcgatcagt
      601 ctgatcgcct cggctgccgc gatcgggacg ctggtcaccg acgtcccggc cggagctctc
      661 ctccacaaga tcgggctgcg tcggtcgatg acgatcggtg cgacgatggt gatcatcggc
      721 acgctgagtc tcgtgttgcc cctcaatccc gtcgccgtga cgtcgctacg cgtcctcgcg
      781 ggaatcggca cggccctgtg ggagctctca cggcactcgt tcatcacgca ggcggtcccg
      841 cccgctgaac gaggtcgcgc gatctcgacc ttcggcgggc tcaaccgcat cgggatgttc
      901 ggtggacccg cgctcggcgg cttgatcgct accgtcttcg gcgtccatat ctcgttcctg
      961 atcaccggga tcatggcgat tctcgctctt gcttccgcca tgatcttcat gtcgcccgcg
     1021 cacctgcagc agatgcacta cgccggctcg gcccggaatc gctggacgat ggttggcgac
     1081 acgctcagga acaatgccgg agacatggcc gcggcatcga tcgcccagct tttcgcgcag
     1141 atgatccgcc aggggcgcca gctcctgatt ccgctttacg gcgcaaccga actcggcctc
     1201 aaccccgccc acgtcggtct cgtgatgacg atctcgtcga tggtcgacat gtcgatgttc
     1261 ttccccgccg gcatcatcat ggatcggtac ggcaggaagt tcgcctccgt tcccagcttc
     1321 gcgatcatgg cggtcgggat cggtatcgtc ccgttcgcct cgtcgttctt cgaactgatt
     1381 ctcgtcggca tcctgatcgg tctggggaac ggcctcgggt ccggcgcgat gatgacactc
     1441 ggcgccgacc tcgcgccgcc gaaggcgacc ggcgaattcc ttggtatctg gcgcctgatc
     1501 ggcgatgtcg gcagcgtcgt cgggccgctg acggtcggag tcgtcggcga atggctcgga
     1561 cttcacggaa gctcgatctt cctgatgttt gcgggtctct gctcgtctgt tatcttgctg
     1621 aaactggtca aggagacgcg ccagatcaag caaccggaac cggtcagcgt ctcataacgc
     1681 ttcacgctcg taccgatctc gaaaggaata ccccctcatg cctctcgtcg cggcacaaat
     1741 cgctcctcat ggttgggaca tcgtcgacgg attcacggag gacgccggcg gtgcgctcac
     1801 gactcgcgcg tcgatgaagc aggtcggcaa gctcttccac gatgcgggag tcgaggtcat
     1861 cgtgctcgtc gggcctcacg gcacccgtgt cgagggtgcg atggccctga ccaacgtcgc
     1921 ccgcggcgcc ggcacgatga tccggcaggg attgacctgg gagacgaacg tcccggtcga
     1981 tcgtcagttc gtgctcgaga tcgatcgccg cgcgcaggcc gccggcgtca acacggcgct
     2041 ggtcggctac gccggaaatc gcgcagacca gagcgcgatc gtgatggact ggggcgcgat
     2101 cgtcccgctg acgttcaacg gtcatccgga gaaccgtgtt ggcttcggga cggtcctcgg
     2161 cggagttccg gaggacgacc acggtcctca ggtcgtgctg atcaccccga gccgagccat
     2221 gggtcgccag gcgatggttg atttcggtcg cgtcctgggt gaggcattcc gtgatgatcc
     2281 gcgcaagatc ggcttcatcg cctcctgcga ctggggacac gcccacacag ccgacggccc
     2341 gtacggattc catcccgatg ccgccaaggt cgatgcgaag gtcgtcgacg cgatcgagaa
     2401 gaacgagctc ctctcgctga tcg
//
LOCUS       DASTMD010000148         6494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2602931, whole genome shotgun sequence.
ACCESSION   DASTMD010000148 DASTMD010000000
VERSION     DASTMD010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2602931"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VFQ54_02270"
     CDS             complement(<1..1062)
                     /locus_tag="VFQ54_02270"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEU0163838.1"
                     /translation="MNEIALSRDEARWLAVTAQGLDRRPFRRKPTKAEILATIRKIGC
                     VQLDTISVISRSHETVLWSRLGSFNPALLAELYDPDHALTEYWAHAAAILPVELMPYF
                     RSYMEWHEEKYGWAADPENRAMMDRLIATMEKDGPISSLDFDAPEEKSELTHAWDWYG
                     KKPEREALNVMWTQGTVMLRRRDGFRRVFDLPHRIAPAVWEGPMMSDDERHRGLVTRA
                     LSALGVATPRWTADYFRSGSPRHVPPKAAKDWLVRLADEGIAIPVTVPGIDEPVWMDA
                     ALVERLELMREGKQRPTLTTLLSPFDNLTWNRPRGEQLWDFHYRIEVYTPAHKRVYGY
                     YSLPILHRGKIVGRLDPSFD"
     gene            1205..2311
                     /locus_tag="VFQ54_02275"
     CDS             1205..2311
                     /locus_tag="VFQ54_02275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0163839.1"
                     /translation="MRVTLVHDYLTQYGGAERVLEAMHDLYPEAPTYTSVYAPDHLPR
                     DFASWDIRTGLIDRLPGAAATHRLWLPLYPATFSRIGESIGDADVILADSSAWSHHVA
                     MTTRIPVICYCHSPARFLYGDRDYLESTAAQRLARYALAPLFGNLRGRDRRAAARVRR
                     YVTNSQAVADRVMSCYGIPAEVIYPPIETTRFRPAQLADPEDWFLVVSRLVPHKWIDR
                     AVRACTEAGFRLKIIGEGRARADLQRIAGPGIEFLGQLGDAAVVDHMQRCRALILPGV
                     EDFGMTAVEVQAAGRPVIAARGGGALETVVDGETGFFFDPADPASLTGALRAASTREW
                     DSAAIVEQTKRFDIARFNREIRAVVDEVATEGAR"
     gene            complement(2321..3085)
                     /locus_tag="VFQ54_02280"
     CDS             complement(2321..3085)
                     /locus_tag="VFQ54_02280"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEU0163840.1"
                     /translation="MSSSSEQPRDDATAHASIIVVIPTYNERENLPGIVGEVLDLGDG
                     YSVLVVDDDSPDGTGQLADDLARQHPGRVDVLHRKTKEGLGRAYVAGLTRAIAKGGDL
                     IVQMDADHSHQPSDLARMVAAAADYDLVLGSRYIAGGDTHGWPWHRKLISRAGGIYAG
                     LVLSVPIRDLTGGFKVWKRATLEAIDLQSIHSDGYSFQIETTYRALTRHFTVKQVPIT
                     FTDRVAGKSKLSRSVVIEAVIMVWRLRWRQVRHKPW"
     gene            complement(3093..3476)
                     /locus_tag="VFQ54_02285"
     CDS             complement(3093..3476)
                     /locus_tag="VFQ54_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0163841.1"
                     /translation="MSSRILIVEDQADVAQLIEVVLVGEGHTVAVAPDGAQGLMMARD
                     WEPDLILMDIMLPGVDGGTLISRLRREKDTAGLPIIAMSASRTLRDRTAELEADALLS
                     KPFDVDALLVQVQFLLERGRSDSEQ"
     gene            4047..4727
                     /locus_tag="VFQ54_02290"
     CDS             4047..4727
                     /locus_tag="VFQ54_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163842.1"
                     /translation="MLRTVPARRVAWSLTVVTLLLIGSLGLAQGQSGYPTMAISTPTT
                     RPGPTAQEDFGCSLQIAGTDFDRNLYGTCTVSDPWWYSFSCAQTPIQGEPWKPAADLP
                     VDTSFVWHPWLKLPSSHGEVVVTLWWGNRPIPAANASGVSPFGKFSISVTFPVPVRSV
                     ELVATTPQGGSSLKTAGEVYEYFHDARIWNVDEIRPPVAGCWSYTILATTEQGDKMNL
                     PFTFVVTD"
     gene            complement(4855..6195)
                     /gene="murA"
                     /locus_tag="VFQ54_02295"
     CDS             complement(4855..6195)
                     /gene="murA"
                     /locus_tag="VFQ54_02295"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU0163843.1"
                     /translation="MIGTTAPSIDSSLDAAERILRIRGNHRLTGKVRIGGAKNAALKA
                     LAATLLTTDEVVLNNVPMIADVISMAELLRAFGADVEIDSERERVVVRAKEIVRTDAP
                     PKLFKATRASVVVTGPILARCGEISFSMPGGDDIGKRPIDMHLRGFMRMGAEVTQDQE
                     MIVARASRLQGARIYMDYPSHTGTEALMMASTLATGTTVIANASAEPEIVSLGEMLNR
                     MGARISGLGTSIITIEGVERLHGCSDVCIPDRLEAGTFAIAAVITGGEVRLENVREAH
                     MLPLTEKLKEAGAEVWFDDNRMLVRAGDRLDSVDIQTLPFPGFPTDLQATFMPLLTQA
                     NGVSRVHERVYEDRLRYTDELIKMGADIHVARWGNHGEMLATSATVRGPVKLHGAHVR
                     SLDIRSAVSLVLAGLVADGETELSDVYHIDRGYARFVEKLTELGADIDDTFLGA"
BASE COUNT         1144 a         2138 c         1992 g         1220 t
ORIGIN      
        1 atcgaacgac ggatcgaggc ggccgacgat cttcccccga tggaggatcg gaaggctgta
       61 gtaaccgtag acccgcttgt gcgccggtgt gtagacctcg atccggtagt ggaaatccca
      121 gagctgctcg ccacgcgggc gattccaggt caggttgtcg aagggcgaga gcaacgtcgt
      181 caatgtcgga cgttgcttcc cctcccgcat cagttcgagt cgctcgacga gcgcggcatc
      241 catccagacc ggctcgtcga tcccgggaac cgtgaccgga attgcaatac cctcatccgc
      301 aagccgaacc agccaatcct tcgcggcttt ggggggaacg tgccgcgggc tgccggaccg
      361 gaagtagtcc gccgtccagc gcggcgtcgc gacgcccagc gcgctcagcg cccgcgtgac
      421 gagaccacga tgacgctcgt cgtccgacat catcggtcct tcccacacgg cgggtgcgat
      481 gcgatgcgga aggtcgaaga cgcggcggaa tccgtcacgc cggcgcagca tcaccgtgcc
      541 ctgcgtccac atgacgttga gggcctcccg ttccggcttc ttgccatacc aatcccaggc
      601 gtgggtcagc tcggacttct cctccggggc atcgaaatcg agcgacgaga tcggaccgtc
      661 cttctccatc gtcgcgatca gccgatccat catcgcccga ttctcgggat cggcggccca
      721 gccatatttc tcttcgtgcc attccatgta cgatcggaag tacggcatca gctcgacggg
      781 aaggatcgcc gcggcgtgag cccagtattc ggtcagcgca tgatcgggat cgtagagttc
      841 ggcgagcagg gcggggttaa aggatccgag gcgactccag aggaccgtct cgtgcgatcg
      901 actgatcacg ctgatcgtat ccagctggac gcacccgatc ttccggatcg tcgccaggat
      961 ctcggccttc gtcggtttgc gccggaacgg acgccggtcc agtccctgtg ccgtcaccgc
     1021 gagccacctg gcctcgtcgc gactcaacgc gatctcgttc atcttcactc cccgtgacct
     1081 gtcgcgccga ccagcaatcg tcgccgaata gaacgcatgt ttgcacgact atactaagcc
     1141 gcgacaatcg cttccaccca atcgctcacc agcgattcct cgtccgggaa ggatccctcc
     1201 tgtcatgcgt gtgacgctgg tccacgacta cctgacccag tacggcggcg ccgaacgcgt
     1261 tctcgaggcc atgcacgatc tctacccgga agctcccacg tacacgtcgg tgtatgcacc
     1321 cgatcatctg ccccgcgact tcgcatcctg ggacatccgc acgggcctca tcgatcgcct
     1381 ccccggcgcg gcggcgaccc atcgcctgtg gctgccactt tatcccgcta cgttttcacg
     1441 tatcggcgaa tcaattggcg acgcggatgt catcctcgcc gattcgagcg cgtggagtca
     1501 tcacgtcgcg atgacgacca gaatcccggt gatctgctac tgtcattcgc cagcccgctt
     1561 tctctacggc gatcgcgact atctggaatc cacggcagcg caacgactcg cgcggtacgc
     1621 tctcgcgcca ctcttcggaa acctgcgcgg gcgagatcgc cgcgccgccg cgcgcgttcg
     1681 tcgctacgtc accaattcgc aagcggttgc agatcgtgtt atgtcgtgtt acggaattcc
     1741 cgccgaggtc atctacccgc cgatcgaaac gacacgattc cgcccggcgc agctcgcgga
     1801 tccggaggac tggttcctcg tcgtctcacg gctcgtcccc cacaagtgga tcgaccgcgc
     1861 cgtccgcgcc tgcacggagg cgggattccg cctcaagatc atcggtgaag gccgcgcccg
     1921 cgccgatctc cagcggatcg ctggacccgg gatcgagttc ctcggccaac tcggtgatgc
     1981 cgcggtcgtc gatcacatgc agcgctgccg ggcgctgatt ctgccgggcg tggaggattt
     2041 cgggatgacg gcggtagagg tccaggcggc ggggcggccc gtcatcgccg cgcggggcgg
     2101 cggcgcgctc gagacggtcg tcgatggaga aaccggattc ttcttcgatc cggccgatcc
     2161 ggcatcgctc acgggtgccc tgcgagcggc ctcgacgcgg gaatgggact ccgcggcgat
     2221 cgtcgagcag acgaagcgat tcgacatcgc gcgcttcaat cgggagattc gggcagtcgt
     2281 cgacgaggtt gccaccgagg gcgcccggtg attgccgttt ctaccagggc ttgtgcctga
     2341 cctgccgcca gcggagtcgc cagaccatga tcacggcctc gatcacgaca cttcgtgaga
     2401 gcttcgactt tcccgcgacg cgatccgtga acgtgatcgg cacctgcttc acggtgaagt
     2461 gccgggtcag cgcccggtag gtcgtctcga tctggaagct gtatccatcg gagtggatcg
     2521 actgcaggtc gattgcttcc agggtcgccc gcttccagac cttgaagccg ccggtgagat
     2581 cccggatcgg cacgctcagc accagcccgg cgtagattcc gccggcgcgg ctgatcagct
     2641 tccggtgcca cggccagcca tgcgtatcgc caccggcgat atagcggctc cccaggacga
     2701 gatcgtaatc ggccgcggcg gccaccatcc gcgccagatc gctcggctga tgcgaatgat
     2761 cggcatccat ctgcacgatg aggtcgccac ccttcgcgat cgcccgcgtc aggcccgcga
     2821 cataggcgcg tccgagacct tccttcgtct tgcgatggag gacgtcgacc cgacctggat
     2881 gttgcctggc gaggtcgtcg gcgagttgtc ccgtaccgtc cggagaatcg tcgtcgacga
     2941 cgagcacgct gtagccatcg ccgagatcga ggacttcgcc cacgattccg ggaagatttt
     3001 cgcgctcgtt gtaagtggga atgacaacga tgatcgatgc gtgagccgtc gcgtcatcgc
     3061 gcggctgttc gctgctgctg ctcaatggct actcactgtt cggagtcgct ccgtccgcgt
     3121 tccagcagga attgcacctg cacgagaagg gcatcgacat caaagggctt ggagagcaag
     3181 gcatcggcct cgagctcggc ggtgcgatcg cgcagcgttc ggctggccga cattgcgatg
     3241 atcggcaatc cggcggtatc cttctcacgg cggagccgcg agatcagcgt gccaccgtcg
     3301 acaccaggaa gcatgatatc catcaggatg agatcgggtt cccagtcgcg ggccatcatc
     3361 aggccctggg cgccgtccgg cgccaccgca accgtgtgtc cctcaccgac caataccact
     3421 tctatcaatt gagcgacatc cgcctgatct tcaactatca gtattcgtga actcaccggg
     3481 tagatacccc ttcagatccg cggttcaagg cgacgaaaaa cgacgcatcc gacgtccgcc
     3541 accatgcacg cgatagcaac gcatgcaacc actgacttct cgacctgagt cccgagacgg
     3601 gaatcggctg gacgtcccag catgttctcg tcacgtacga acaatatagg cgaaacgggg
     3661 ctcccggcaa gcgattcggc atcttacgaa cgcatcgcca ccaatccgct tcatgtttac
     3721 agcgctcccc tactaccttt tgccttgcca cgattccggt caggtctgac ggcccggtat
     3781 gcgcacatcg gctttcgtcg attgtcgggg ccgctgaagt ctcggatcga gggccttgaa
     3841 ccacgcgttg ctatctggca aggatcaccg acaatgtgcg taaccttttc cacgtggagt
     3901 tcaacggaga aacagagggt cccgcccaac tcggcggacc cgggcgtcgc gtagagttgg
     3961 tccattggtc tatacggatc tgtgggccca ggtggttgct ctaatccact cgtgttgggt
     4021 cagtagccac gcgaaaggtg gcagcaatgc tcaggacagt tccggctcgg cgggtcgcct
     4081 ggtcactgac agtggtgacg ctgctcctga tcggcagtct tggcctcgcc caaggacagt
     4141 ctggctatcc gaccatggcc atcagcacgc caacgacgcg ccccggcccg acggcccagg
     4201 aggatttcgg atgctcgctc cagattgcgg gaacagattt cgatcgcaac ctctatggca
     4261 cctgcaccgt ttccgaccct tggtggtatt ccttttcctg tgcccaaacg ccgattcagg
     4321 gggagccatg gaagcccgca gctgatctcc cggtggacac ctcgttcgtt tggcatccgt
     4381 ggctgaagct tccgtctagc cacggagagg tcgtcgtcac actttggtgg ggcaatcgcc
     4441 cgatcccggc tgcgaacgcc agcggcgtca gcccctttgg aaagttttcg atttctgtca
     4501 cctttccggt gccggtcagg tcggtcgaac tcgtcgcaac gaccccacag ggcggctcgt
     4561 ctctaaaaac tgcgggcgaa gtgtatgagt actttcacga tgcccgtatc tggaacgtcg
     4621 atgagatacg accgccggtc gccggatgct ggtcgtacac gatcctcgcc acgactgagc
     4681 aaggggacaa gatgaacctg ccattcacct tcgtcgtgac ggattagcga ggtgactcat
     4741 gaacgtaagc cattcccgtg aagtccgatt gaggcgagac tccgttgcgc cggcgccgtt
     4801 gctcgtcctg atcccatagt cactcgcccg acgagcggga tgagaccggg gcgttcaggc
     4861 tcccaggaag gtatcgtcga tatcagcgcc gagctcggtg agcttctcga cgaaccgggc
     4921 gtagccgcgg tcgatgtgat agacgtcgct caactcggtc tcgccatcgg cgacgagtcc
     4981 cgcgaggaca agactcaccg ccgagcggat atcgagcgag cggacgtgcg caccgtggag
     5041 cttgaccggg ccgcgcaccg tcgccgacgt ggcgagcatc tcgccgtgat ttccccaccg
     5101 cgcgacgtgg atatcggctc ccatcttgat cagttcatcc gtgtaacgca gccggtcctc
     5161 gtagacccgc tcgtggacgc gggagacgcc gttcgcctgc gtcagcagcg gcatgaatgt
     5221 cgcctggaga tcggtcggga agccgggaaa cggcagcgtc tggatatcga cggagtcgag
     5281 ccgatcgccc gcgcggacga gcatccggtt gtcgtcgaac cagacctctg cccccgcctc
     5341 cttcaacttt tcggtgagtg gcagcatgtg agcttcgcga acgttttcga gccgcacctc
     5401 gccaccggtg atcaccgccg cgatcgcgaa ggtgccggcc tcgagccgat cgggaatgca
     5461 gacatcggaa cacccgtgca ggcgctcgac gccctcgatc gtgatgatcg acgtcccgag
     5521 gccggaaatg cgggcgccca tccggttcag catctcgccg agggagacga tctccggctc
     5581 cgcggacgcg ttcgcgatca ccgtggttcc cgtcgcgagc gtggaggcca tcatcagcgc
     5641 ctcggtcccg gtgtggctcg ggtagtccat gtagatccgg gcgccctgca ggcggctggc
     5701 gcgcgcgacg atcatctcct gatcctgggt cacctcggcg cccatccgca tgaagccgcg
     5761 aagatgcatg tcgatcggac gcttgccgat gtcatcccca cccggcatcg agaacgatat
     5821 ctcgccacat cgggcgagaa tcgggcctgt gacgacgacc gaagcccgcg tcgccttgaa
     5881 gagcttgggc ggtgcatcgg tgcgcacgat ctccttcgcc cgcacgacaa cgcgctcgcg
     5941 ctcggaatcg atctccacgt cggcgccgaa cgcgcgcagc aactccgcca tcgagatgac
     6001 gtcggcgatc atcggcacgt tgttgagcac gacctcgtct gtcgtcagca aggtcgcggc
     6061 aagcgccttc aaggcagcgt tcttcgcgcc gccgatccgt accttgccgg taagccggtg
     6121 gttgccgcgg atgcgcagga tgcgctcggc ggcgtcgagg ctggagtcga tggatggcgc
     6181 ggtggttccg atcatgatgg ccctctcctc gcgcaaacgc acgggaacgt cacgcatcgt
     6241 gcgtctggga tgtgctggcg gggatctcgc ccgaacgtcg ggcgcggtgt gtgccagcct
     6301 ggcgggagca tggcaacgga tggtgattca ggacagatgt ggctccgggt cggcccgcgg
     6361 atgcgcctcc cagtatacga gagtggcttc gatcgctgcc cgggagtcac acccgtcgcg
     6421 acaccgcccg ctgccgccgc gtctcgatcc gtcggcgagc ctccgcatcc tctccacaac
     6481 cagtcgattg gaga
//
LOCUS       DASTMD010000149         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1442279, whole genome shotgun sequence.
ACCESSION   DASTMD010000149 DASTMD010000000
VERSION     DASTMD010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442279"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFQ54_02300"
     CDS             <1..243
                     /locus_tag="VFQ54_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEU0163844.1"
                     /translation="EGSRAAAILYRTDDGWRVSMRSMTSDVDVAAIAARFGGGGHPRA
                     AGCQIHGGVEARDSFLSQVAEILAEPAPASISPANA"
     gene            285..1151
                     /gene="truB"
                     /locus_tag="VFQ54_02305"
     CDS             285..1151
                     /gene="truB"
                     /locus_tag="VFQ54_02305"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU0163845.1"
                     /translation="MIDKPGGWTSHDVVSRVRRILGERRVGHAGTLDPAAVGVLPIAV
                     GLATRTVEYLSEADKSYRAEITFGLETDSADGDGVVIGVARAVTVGEIELRNALGDFV
                     GPQGQVPPMHSALKVDGKRLYELARAGEEIEVQPRTITIHAIALVNWAPPVATVDITC
                     SKGTYVRSIARDLGRRLGTGAYLSNLVRTSTGPFVLRDAMRLGELEEFAGRGTDWAQM
                     AFHPDTVLLDRPAVILDPESALAWIQGKVVAQPGCSGTIRVYDDAGNWLGVGTADATS
                     QTIKADKVVPTE"
     gene            1175..2128
                     /locus_tag="VFQ54_02310"
     CDS             1175..2128
                     /locus_tag="VFQ54_02310"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU0163846.1"
                     /translation="MFTSFDALPEGATVATIGTFDGVHRGHQHLLTVVRDRARELDVR
                     SLVVTFEPTPAQFFRPASFAGRLVTPEQKVELILGCGIDAVLVHPFDQAFADLTPAAF
                     MTTLVESGHPRELWIGADFALGHARAGNADVLTSLGADLGVAVKVIDRVDIDGTAVSS
                     SAIRKLVAAGEIDQANTLLGHRFAIPGEVIKGAQVGRTIGYPTANVPLPEDLVLPSDG
                     IYATYAQIGDDPAQRPAMTYIGTRPALNTGSRLIETHLIDFVGDLYGQELTTSFVRRL
                     RPDSDFVSVELLVKQLQDDEVHTREVLGLAPATALSEISEV"
     gene            2187..2819
                     /gene="infC"
                     /locus_tag="VFQ54_02315"
     CDS             2187..2819
                     /gene="infC"
                     /locus_tag="VFQ54_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU0163847.1"
                     /translation="MNRLFCMSDKYRQQFRRQAISRPVQTRVNERIRIREVRLIDDEG
                     RQIGIIPTREALEMARERGLDLVEVAPNAVPPVCRLMDYGRFRYEQSRKERESRKSQH
                     VTRVKEVRVEPQISSHDLETKGRQAAKFLDEGDKVKLSILFRGRRITHPELGRDLLDQ
                     LQEQLKDHGTVEQTARMEGRTLTMHMAPLRARPAGEKREDSSDSEQQDQA"
     gene            2797..3006
                     /gene="rpmI"
                     /locus_tag="VFQ54_02320"
     CDS             2797..3006
                     /gene="rpmI"
                     /locus_tag="VFQ54_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU0163848.1"
                     /translation="MSNKIKLKTHKGAKRRFKITATGKIMHAKGMKSHNRRKKAPRVL
                     RQLDRMQEMDSTNAKRMKLLLPNGL"
BASE COUNT          604 a          889 c         1038 g          561 t
ORIGIN      
        1 gaaggatcgc gggcggccgc gatcctctat cgcacggatg atggctggcg tgtcagcatg
       61 cggtcgatga cgagcgacgt cgatgtcgcg gcgatcgccg cgcgtttcgg tggcggcggt
      121 catccccgcg ccgcgggctg ccagatccac ggcggcgtcg aggcgcgtga ctcgtttttg
      181 agtcaggtcg ccgagattct cgctgaacca gcgccagcct cgatttctcc cgccaacgcc
      241 tgatgccacg caatcgccga agcgccaacc gccatggatg gatcgtgatc gacaagcccg
      301 gtgggtggac gagtcacgat gtcgtcagcc gggtgcgacg gatcctcggc gagcggcgtg
      361 tcggccacgc gggaacgctg gatccagctg cggtgggggt ccttccgatc gccgtgggac
      421 tggcgacgcg gacggtcgaa tatctctccg aagcggacaa gtcctaccgc gctgaaatca
      481 cctttggtct cgaaacggat tccgcggacg gcgatggcgt cgtgatcggt gtcgccaggg
      541 cggtcacggt tggcgagatc gaattgcgca atgcgctggg cgacttcgtc ggaccccagg
      601 gccaggtgcc gccgatgcat tcggcgctga aggtcgacgg caagcgattg tacgaactcg
      661 cccgggcggg ggaggagatc gaggtccagc cacgaacgat cacgatccat gcgatcgcgt
      721 tggtgaactg ggcgccgccc gtcgcgacgg tcgatatcac gtgctccaag ggcacgtatg
      781 tgcgatcgat cgcccgggac ctcggacgcc ggctcgggac cggtgcatac ttgtcgaacc
      841 tggtgcgcac atcgacaggt cctttcgtat tgcgagacgc gatgcggttg ggcgagcttg
      901 aggagttcgc aggccgtggc actgattggg cgcagatggc gtttcatccg gacaccgttc
      961 tgctcgaccg tccggcggtg atcctcgatc cggaatcggc gctcgcgtgg attcagggga
     1021 aggtggtcgc gcagcctggt tgttcgggca cgatacgggt gtacgatgac gcgggtaact
     1081 ggctcggtgt cggtacggcc gacgcgacca gccagaccat caaggcagac aaggtcgttc
     1141 cgacagagtg agaactgatt ccgtgggaaa cgctgtgttc acttcattcg acgcgctgcc
     1201 ggaaggtgcg accgtcgcga ccattggaac gttcgacggc gtccatcgcg ggcaccagca
     1261 cctgctgacg gtcgttcgcg accgggcacg cgaactcgat gtccgctccc tggtcgtcac
     1321 gttcgagccg acgccggcgc agttcttccg cccggcctcg ttcgcgggtc gactggtgac
     1381 accggagcag aaggtcgaac tgatcctcgg ttgtggaatc gacgccgttc tggtgcatcc
     1441 cttcgaccag gcgttcgcgg acctcacacc ggccgcgttc atgacgacgc tggtcgaatc
     1501 cggtcacccg cgggagctct ggatcggcgc cgacttcgca ctcggtcacg cgcgggcggg
     1561 caatgccgac gtcctgacct cgctgggcgc ggatctcggc gtggcggtga aggtcatcga
     1621 tcgcgtggat atcgacggga cggcggtcag cagcagcgca attcggaagc tcgtcgccgc
     1681 aggcgagatc gaccaggcga acacccttct cggtcaccgg ttcgcgattc cgggagaggt
     1741 gatcaagggt gcccaggtcg gtcgcacgat cgggtatcca acggcgaacg tgccgctgcc
     1801 ggaagacctg gtcctgccgt cggacggcat ctatgcgacc tacgcgcaga tcggtgacga
     1861 tcctgcacag cgaccggcga tgacgtacat cggaacccgg ccggcgttga acacgggaag
     1921 cagattgatc gagacgcatc tgatcgattt tgtgggtgat ctctacgggc aggaattgac
     1981 gacgtcgttc gtccggcggt tgcgcccgga ctcggatttc gtcagcgtcg aactcctggt
     2041 caagcaattg caggacgacg aggtacatac ccgggaagtc ctgggattgg cgcctgcgac
     2101 ggcactttcg gagatctcgg aagtttgatt cggtcataac atcatggtat tcttgacagg
     2161 cgttgtcgct gacggaacgt cggtctatga accggctttt ttgtatgtcc gacaagtacc
     2221 gtcagcagtt caggaggcaa gctatttctc gaccagtaca gactcgcgtg aatgagcgaa
     2281 ttcgcatccg cgaggtacga ctcattgatg acgaagggcg ccagatcgga atcatcccga
     2341 cgcgagaagc gttggaaatg gcgcgcgagc ggggactcga cctcgtcgaa gtggcgccta
     2401 atgcggtgcc ccccgtttgc agattgatgg actacggcag gttccgatac gagcagagcc
     2461 gtaaagagcg tgaatcccgt aagagccaac atgtgacgcg cgtcaaggaa gtccgggtcg
     2521 agccgcagat cagctcgcac gacctggaga cgaagggccg tcaggccgcg aagttccttg
     2581 atgaaggcga taaggtaaag ctcagtattc tgttccgagg acgtcgcatc acccacccgg
     2641 agctcgggcg tgatttgctc gatcaacttc aggaacagct gaaggatcac gggacagtcg
     2701 aacagacggc tcggatggag ggccgcacac tgaccatgca catggcgccg cttcgggccc
     2761 ggcctgcagg tgagaagaga gaggattctt ccgacagtga gcaacaagat caagcttaag
     2821 acccacaagg gtgccaagcg ccgtttcaag atcacggcga caggcaagat catgcacgcg
     2881 aagggtatga agagccacaa ccggcgcaag aaggcgccgc gagtgctccg ccagttggat
     2941 cgcatgcagg aaatggactc gacgaacgcc aagcgcatga agcttctgct tcccaacggg
     3001 ctttaggcga actagaaccg atccggtgcg attcgtgtgt gatcggagca gaggttaaac
     3061 agagatggcc agagtcaagc gtggtgttgc gg
//
LOCUS       DASTMD010000150         5213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1977372, whole genome shotgun sequence.
ACCESSION   DASTMD010000150 DASTMD010000000
VERSION     DASTMD010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977372"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFQ54_02325"
     CDS             <1..360
                     /locus_tag="VFQ54_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163849.1"
                     /translation="AIAAARGGWAKAISAPIGRLATFLTDQLAIPFEGILRANITGEE
                     VARTVTSVIVIIVIAMIWYTIAVLPSWERWNATQVNRFFRSQRPDTDRGSEYFRWVRW
                     SVPIVVGLSLWAIASIH"
     gene            496..1896
                     /locus_tag="VFQ54_02330"
     CDS             496..1896
                     /locus_tag="VFQ54_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0163850.1"
                     /translation="MALTTNTTSAGRPGGDDDPGYRWRPGLTPVSLFLVLLLFWIMTQ
                     IQIVMVLGLLALLFGTILEGPVALLERRRIPRPAAIAMVYAVIIGGLALLIIAIIPVI
                     ADQADTFREEVPNQIRQLEHDWRASSNPLLNGVGADGLQRALDFFDKPSNGVSGDTAE
                     RILPVVISIGTGIVSTIGLLVIVFYYLMEKALIRRVIIAQLPERSQTRVDRVWGDVER
                     KVGGWMRGQLLLCLIIGTIATVSYGIIDLPFWPLLGLWAGMTEILPIVGPWIGGVPAV
                     ILALTVGWDKAIVTAIIIIAMQTLENWFLVPRVMRGAVGLTPLTVFIAILAGQQFLGI
                     IGAVLAIPVAAAIQVIITDWLNARRQHDPTPAVSSWRWMLSRSAREDVSTGEPLPAQD
                     AVPSPPPADHQEQIVSDVAPRASVTTDPPTGDPAAVPNPRTEPPAVDRTWSWQKKTLN
                     PNQEISKRNDNEHPAK"
     gene            2040..2822
                     /locus_tag="VFQ54_02335"
     CDS             2040..2822
                     /locus_tag="VFQ54_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU0163851.1"
                     /translation="MSSSHKIKISVQVQPQQGTYEKQRAAWIAADELGVDVIFNWDHF
                     YPLYGEPDGAHFEAYTLLAAMAEVTERAQIGSLVTCNSYRNPNLLADMSRTIDHISGG
                     RFILGIGSGWAERDYFNYGYEFGTAASRLQDLARDLPIIEDRFAKLNPGPVNGSIPIL
                     IGGDGAKVTLRLVAKHARIWHAFGDVETITRKSAILADWCAKIGRDPQEIERSTGISK
                     ETQSNLDAYIAAGVTHFTIGTGGPDFDLGLVKELVAWRDSRN"
     gene            3007..3807
                     /locus_tag="VFQ54_02340"
     CDS             3007..3807
                     /locus_tag="VFQ54_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU0163852.1"
                     /translation="MTEPTSRPQQLKVSVQVGLQLVPYAEQRAAWIESERLGVDTIFN
                     WDHFFPSLPGADADGTNLEAYTSLAAMAEVTERVQIGCLVTCNSYRNPNLIADMARTI
                     DHISGGRFILGMGSGWMVRDYEEYGYELGTPASRLQALARDLPIIEDRLGKLNPGPVN
                     GRMPLLIGGGGEKVTLRLVAKHADIWHGGGDVETIARKVSILDEWCAKIGRDPHIIER
                     SVGINAQQIADLDKYVSLGVTHFTTTLGGPTYDYGLLRELLAWRDSHR"
     gene            4051..5190
                     /locus_tag="VFQ54_02345"
     CDS             4051..5190
                     /locus_tag="VFQ54_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0163853.1"
                     /translation="MHVVQIAPPWFPIPPTGYGGIERVVHDLTEGLLAAGCEVTLCAP
                     AGSMTNANLIPTVARPVGLNLTEAQKSRHFQRASRAAYKAALEIGADIIHDHTDHIPQ
                     RTFPVPIVRTVHGPATEAAMLLYREASRRGDHLVAISQRQRQLFLKRAAERWGDGVTL
                     NFDDVIHNPIDIDGTPFYPAGEKRGYAAFLGRCHWEKSPDGAIRVAQTADVPLMMALR
                     VTTEEQPYFEAVVAPLTRSIKNLAKLVGEVTGHEKDELIGHAGVVLFPSPWEEPFGLV
                     LTEAGARGTPVVTLARGSAPEIVVDGVTGILCADEEEMARAIPRAMALDPEACRAHVA
                     ARFNRELIAGQYLDLYERILARQADIQAGPRELAPALGQLRPAGS"
BASE COUNT          961 a         1769 c         1591 g          892 t
ORIGIN      
        1 gcgatcgccg ccgcccgtgg cgggtgggcg aaagcgatca gcgccccgat cgggcgcctc
       61 gccaccttcc tcaccgacca gctggcgatt cccttcgaag gaatcctgcg agcgaacatc
      121 accggagaag aggtcgcgcg aacggtgacg agcgtcatcg tcatcatcgt gatcgcgatg
      181 atctggtaca cgatcgccgt cctgccctcg tgggagcggt ggaacgcgac gcaagtcaac
      241 cggttcttcc gaagccagcg accagacacc gatcgcggga gcgagtactt ccgctgggtt
      301 cgctggtcgg tgccgatcgt cgtcggcctg tcgctctggg cgattgccag cattcactga
      361 cttccagaac gcatcgaccc gatcctcatc gaacgcatcg gggtacagtt agctgtgccc
      421 gcgcgttgtt cgggaagatg tttcgtccac gtttcccacc gcccatgagg cgcattgtga
      481 ggagagtttg ccgaaatggc attgacgacc aacaccacct ctgctggtcg cccgggaggc
      541 gatgatgacc ccggctaccg gtggcggccc ggcctgaccc cggtctccct cttcctggtc
      601 ctgctcctct tctggatcat gacccagatc cagatcgtga tggtcctcgg ccttctcgca
      661 ttgctcttcg gcacgatcct cgaaggaccg gtcgccctcc tcgagcgacg acggattccc
      721 cgtcccgccg cgatcgcgat ggtctacgcc gtgatcatcg gcggtctcgc gctgctcatc
      781 atcgcgatca tcccggtcat cgccgatcag gcggacacgt tccgcgaaga ggtgccgaac
      841 cagatccgcc aactcgaaca cgactggcga gcgagctcga atccgctcct gaacggcgtc
      901 ggggcggatg gcctccagcg cgcgctcgat ttcttcgaca agccatcgaa cggcgtctcc
      961 ggcgacacgg ccgagcgcat cctgccggtg gtcatctcga tcggcaccgg gatcgtctcg
     1021 acgatcggtc tgctcgtgat cgtcttctac taccttatgg aaaaggcgct gattcgccgc
     1081 gtcatcatcg cccaactacc ggaacgatcg cagacacggg tcgacagggt ctggggcgat
     1141 gtcgagcgca aggtcggtgg ctggatgcgc ggccagctgt tgctctgcct catcatcggc
     1201 acgatcgcga ccgtcagcta cggcatcatc gacctcccct tctggccgct cctcggactc
     1261 tgggcgggga tgacggagat cctgccgatc gtcggcccct ggatcggcgg cgtgccggcg
     1321 gtgatcctcg cgctgaccgt cggctgggac aaggcgatcg tcaccgcgat catcatcatc
     1381 gcgatgcaga cgctggagaa ctggttcctc gtcccgcgcg tgatgcgggg cgccgtcgga
     1441 ctcacgccgc tgacggtctt catcgcgatc ctcgccggtc agcagttcct cgggatcatc
     1501 ggcgccgtcc tcgcgattcc ggtcgcggcc gcaatccagg tgatcatcac cgattggctg
     1561 aacgcccgcc gccagcacga cccgacaccc gcggtctcca gctggcgctg gatgctctcc
     1621 cgctcggcgc gcgaagacgt atcaacgggt gagccgcttc ccgcacagga cgcggtgccg
     1681 agtccgccgc ccgccgacca tcaggagcag atcgtcagcg atgttgcgcc acgagcttcc
     1741 gtcaccactg acccgccgac gggtgatcct gccgccgttc ccaacccgcg gaccgaaccg
     1801 ccagcggtcg atcgaacgtg gagctggcag aaaaagacgc tcaacccgaa ccaggagata
     1861 tcgaaacgga atgataacga acacccggct aagtaatcga cagttgtcgg gtatcgcaat
     1921 aacgaaaaag catcctcgct cgacgtgacc ggaagtggac atatcgctta cgagtggtta
     1981 gccttccgaa gggggcgatg gttgcgccct ctatggtcta gccggaagga ttccttcgcg
     2041 tgtcgagttc gcacaagatc aagatcagcg ttcaggttca gcctcagcaa gggacatacg
     2101 agaagcaacg cgcggcgtgg attgccgcgg atgagctggg cgtggatgtc atcttcaact
     2161 gggatcactt ctacccactc tacggtgagc cggatggcgc tcacttcgaa gcctacacac
     2221 tgctggcggc aatggcggaa gtcaccgagc gtgcccagat cggctcgctt gtcacctgca
     2281 actcctaccg caaccccaat ctgctcgccg acatgtcgcg caccatcgat catatctccg
     2341 gtggccgctt catcctcggc atcggctccg gttgggccga gcgcgactac ttcaactacg
     2401 gctatgaatt cggaaccgcc gcgagccgcc tccaggatct cgcccgcgac cttccgatca
     2461 tcgaagaccg gttcgccaaa ctgaacccgg gcccggtcaa cggaagcatt ccgatcctga
     2521 tcggcggcga tggcgcgaag gtgaccctcc gcctcgtcgc gaagcacgcc aggatctggc
     2581 acgccttcgg cgacgtcgag acgatcacgc gcaagagtgc gatcctcgcc gactggtgcg
     2641 cgaagatcgg acgcgacccg caggaaatcg agcgctcgac gggaatctcg aaggaaacgc
     2701 agtcgaacct cgacgcctac atcgcggcgg gcgtgaccca cttcacgatc ggtaccggcg
     2761 gtccggactt cgacctcggc ctggtgaagg aactcgtcgc ctggcgagac agccggaact
     2821 aatctccagc tagagagccg cgatcgaagc cgtcgccacc gggagcatgt tcctcgggcg
     2881 aacgcgaggc gggacaggcc gtccggactg cggtccggca acggcggtcc cgccctgcga
     2941 acgaccggat gcaggcaacg tccgtctgcg cgcacccttt cgtccttacg aaacgagaac
     3001 cacgacatga ccgagcccac ttcgcgaccg cagcaactca aggtgagcgt gcaggtcggc
     3061 ctccaactcg tgccctacgc cgaacagcgt gcggcctgga tcgaatcgga acgactcggg
     3121 gtcgatacga ttttcaactg ggatcatttc ttcccctccc ttcccggcgc ggatgctgac
     3181 gggaccaatc tcgaggccta cacctcgctc gcggcgatgg cggaagtgac ggagcgcgtc
     3241 cagatcggct gcctcgtcac gtgcaactcc tatcgcaatc cgaatctgat cgcggacatg
     3301 gcgcgcacga tcgaccacat ctccggcggc cgcttcatcc tcggcatggg gtccggctgg
     3361 atggtgcgcg actacgagga gtatggctac gagctcggca cacccgcgag ccgcctccag
     3421 gccctggccc gggatctccc gatcatcgag gaccggctcg gcaagctcaa ccccggcccg
     3481 gtcaacggtc ggatgccgct gctgattggc ggtggtggcg agaaggtgac gctgcgcctc
     3541 gtcgccaagc acgccgacat ctggcacggc ggaggggatg tcgaaaccat cgcgcgcaag
     3601 gtctccattc tcgacgagtg gtgcgccaaa atcggacgcg atccccatat catcgagcgc
     3661 tctgtcggaa tcaacgcaca gcagatcgcc gatctcgaca agtacgtttc gctcggagtc
     3721 acccacttca ccacgacgct gggtggcccc acgtacgact acggactgct tcgcgaactc
     3781 ctcgcctggc gcgacagcca ccggtagcgg tgccgatccg ggcggggcac cgtgtcccgc
     3841 ccgggacact cgtcccggcg atcctcgcgg tcgccgggac gcgactctcc tcgccggatc
     3901 ggcgccccag ccgaatgttg tgatcgcaat ccctaaacgt gctgcatgat ctcaaccgcg
     3961 gcgtggatct tgctgtatat tcgtcgctcc cttcgcctac aaacggagta atcgacagcc
     4021 atcgattccg cctgcgctga aaggcaaccg atgcacgtcg tacagatcgc tcctccctgg
     4081 ttccccatcc cgccgaccgg atacggcgga atcgaacgtg tcgtccacga tttgaccgag
     4141 ggtctgctcg ctgccggatg tgaggtcacg ctctgtgcgc cggccggctc gatgaccaat
     4201 gcgaacctca tccccaccgt cgcgcgtccg gtcggtctca atctcacgga agcccagaag
     4261 tcccgccact tccagcgcgc aagccgtgcc gcatacaagg ccgcgctgga aatcggggca
     4321 gacatcatcc acgatcacac cgaccacatc ccgcagcgga ccttccccgt gccgatcgtc
     4381 cgcaccgttc acggaccggc gaccgaggcc gcgatgctgc tctaccgcga ggcgagccga
     4441 cgcggcgacc atctcgtcgc aatcagccag cggcagcgac agctcttcct gaagcgggcc
     4501 gcggagcgat ggggcgacgg cgtgacactg aatttcgacg acgtcatcca caaccccatc
     4561 gatatcgacg ggacgccctt ttacccggca ggcgaaaagc gcggctacgc ggcgttcctg
     4621 ggacggtgcc attgggagaa aagtcccgat ggagcgattc gcgtcgccca gacagccgat
     4681 gtgccactga tgatggcgct gcgggtgacg accgaggaac agccctactt cgaggcggtc
     4741 gtcgcgccgc tgacgcgctc gataaagaac ctggccaaac tggttggcga ggtcacggga
     4801 cacgagaagg acgaactgat tggacacgcc ggtgtagtcc tcttcccgtc accgtgggag
     4861 gagccattcg ggctggtact tacggaggcg ggagcccgcg gcacccccgt ggtgacgctt
     4921 gcccggggat ccgcacccga gatcgtggtc gacggcgtca cgggaatcct ctgcgcggac
     4981 gaggaggaga tggcgcgggc gattccacgc gcgatggccc tcgatcccga agcgtgccgg
     5041 gcgcatgtcg ccgcgcgttt caatcgcgag ctgatcgccg ggcaatatct cgatctctac
     5101 gagcgaatcc tcgcccggca ggcagacatc caggcggggc cacgcgaact cgcccctgcg
     5161 ctcgggcagc tacggccagc cggtagctga agcatcgtct cgacgggaga ccg
//
LOCUS       DASTMD010000151         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_550129, whole genome shotgun sequence.
ACCESSION   DASTMD010000151 DASTMD010000000
VERSION     DASTMD010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550129"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFQ54_02350"
     CDS             complement(<1..402)
                     /locus_tag="VFQ54_02350"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HEU0163854.1"
                     /translation="MSAPTESTTGVPGIPLPIVALNRWMLVVGIGVAFVFQLPWITTI
                     ILVILLGSIVFGPRGSLVFQVGNRLLAGPVAKAKAGGHVEDRRLMRFNNSIAVILLAI
                     AQVAFLAGTGVVGWVLSLMVVVAAAVALAGFC"
     gene            complement(599..1549)
                     /locus_tag="VFQ54_02355"
     CDS             complement(599..1549)
                     /locus_tag="VFQ54_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0163855.1"
                     /translation="MIRDPEEMKTHLRGALAFPITPYAADGSVDLDAVRENTAFLASS
                     EIAAIVAPSGTGEIFALTPDEAIAIVRATVQSAGNKPVIAAAGFGPRLGAELARASEA
                     AGAAAIMIVPPYYGKPDPQGLLDYYAEIAAATSLGIIPYARDSALLTPKMVEQLVQRV
                     PNAIAFKDGRADVRLFLQIREYVTERLGTDRLVWLAGAGDDLVGPYFAAGAEGFTSSL
                     ACFWPEASVELYRLAANGDYAGLADYHHRVVRPIYAMRQRKPGYEVSVMKAAVELLGF
                     RAGPARPPLANLAPDERDELAVLLRDLAVPTAADRTAAVR"
     gene            1730..>2174
                     /locus_tag="VFQ54_02360"
     CDS             1730..>2174
                     /locus_tag="VFQ54_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163856.1"
                     /translation="MKIREMKVTPIAIADAPLRNVVGIHEPYVNRIIVELVGEDGNSG
                     FGEAAFSNRTWQDLLALRSDVIGHDSLNLNVLSNVVAARLGRPGALDLDPVLRNFELA
                     PRRTNSAVPRTLSPIEVAALDLSGRSLGVPVCDLLGGRVRDAAPFS"
BASE COUNT          399 a          687 c          726 g          362 t
ORIGIN      
        1 gcagaatccg gcgagagcga ccgcggccgc gacgacgacc atcaacgaga gcacccagcc
       61 gacgacaccc gtgcccgcga ggaaggcgac ctgggcgatc gcgagcagga tcaccgcgat
      121 cgagttgttg aatcgcatca agcggcgatc ctcgacgtgc ccgcccgctt tcgccttcgc
      181 gacgggaccg gcgaggaggc gattgccgac ctggaagacg aggctgcctc gcggcccgaa
      241 gacgatcgat cccagcagaa tgacgaggat gatcgtcgta atccacggga gctgaaaaac
      301 gaaagcaacg ccgatgccaa cgacgagcat ccagcgattc agggcgacga tcggaagcgg
      361 aatccccgga actccggtcg tcgattcagt tggagcggac atctggtctg atcaccgatc
      421 actatcgttc ggaaagcttc atgacttcac aatagtgttg gtttgaaaca tcgtcaacca
      481 tgcaaagccc atggattgcg ggatcttttc cccaatccac gggctgcggg ctgcagctgt
      541 aattcggtgc cggatgtttt cgccatggcg cgggtccctg gaccgccgtc ggcccagcct
      601 agcggaccgc agctgtccgg tcggcggcgg tcgggacggc gagatcacgc agcaggacgg
      661 cgagctcgtc acgttcgtct ggagcgaggt tcgccaatgg tggacgggcc ggtcccgccc
      721 ggaaaccaag caactcgacg gcagctttca tcaccgagac ctcatacccc ggtttgcgct
      781 ggcgcatcgc gtagatcgga cgtacgacgc ggtggtgata atcagcgaga ccggcgtagt
      841 cgccattggc agcgaggcgg tagagctcga ccgacgcttc cggccagaaa caggcgagtg
      901 atgaggtgaa gccctcggct ccggccgcga agtagggacc gacgagatcg tcgccggcac
      961 ccgcgagcca gaccagtcga tcggtgccga ggcgctcggt gacgtattcg cggatctgga
     1021 ggaagagccg gacatcggcg cggccatcct tgaaggcgat cgcgttcggg acgcgctgga
     1081 cgagttgctc gaccatcttc ggggtcagga gcgcggaatc gcgggcgtac ggaatgatcc
     1141 cgagcgatgt cgccgccgcg atctccgcgt agtagtcgag caatccctgg ggatccggct
     1201 tcccataata gggcggcacg atcatgatcg cggccgcgcc cgcggcttcc gaggctctgg
     1261 cgagttccgc gcccaggcgt ggcccgaatc cggcggccgc gatcaccggc ttgttcccgg
     1321 cggactggac tgttgcgcgc acgatcgcga tcgcttcgtc cggggtcagg gcgaagatct
     1381 caccggtgcc gctcggggcg acgatcgccg cgatctcact gctcgccagg aacgcggtgt
     1441 tctcacgaac ggcatcgagg tccaccgatc catccgcggc atacggagtg atcgggaacg
     1501 cgagcgcgcc ccgcaggtgg gtcttcatct cctcgggatc gcggatcatg gcgtcctcct
     1561 gacgcgatcg accgccggat cgactcgccc atcgtcgctt gcccggcgtc gaatcctgat
     1621 tgacacgttt cgtctgggat cgataatcgc cgcagacgaa gcacaagtca acgacacggg
     1681 gtcccggcgc cgggagcgtc ggcatctcac ggacggagga gagagacgcg tgaagatccg
     1741 ggagatgaag gtcacgccga tcgcgattgc ggacgctcca ttacgcaacg tggtcggcat
     1801 tcacgagccg tacgtcaatc gcatcatcgt tgaactcgtc ggtgaggatg ggaacagcgg
     1861 tttcggcgag gcggcgttct cgaacaggac gtggcaggat ctgctcgcgc tgcggtcgga
     1921 cgtcattggc cacgactcgc tgaacctgaa tgtgctttcg aatgtggttg cggcccgcct
     1981 cgggcgcccg ggggcgctgg atctcgatcc ggtcctcagg aacttcgagc ttgctccccg
     2041 gcgcacgaac agcgccgtgc cgcgcaccct cagcccgatc gaggtcgctg cgctcgatct
     2101 cagcggacgc tcgcttggcg tccccgtctg tgacctgctc ggcggacgcg tccgcgacgc
     2161 cgcgccgttc agtg
//
LOCUS       DASTMD010000152         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_192690, whole genome shotgun sequence.
ACCESSION   DASTMD010000152 DASTMD010000000
VERSION     DASTMD010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192690"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFQ54_02365"
     CDS             complement(<1..476)
                     /locus_tag="VFQ54_02365"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HEU0163857.1"
                     /translation="MQSRDNLNPPPTDSTRSPQPARPTGINPELILPLRLFLGGMFLY
                     AGIQKLTAANFFNKDGSGSIGEQLHGYVADGSPLSFLIRHIALPNAVLIGVLVALAEL
                     WIGLSTLTGLLPRIGAAGGAAMSLLFFLTASWSIRPVFLGPDLPYMAAWITLYLTGP"
     gene            complement(749..1969)
                     /locus_tag="VFQ54_02370"
     CDS             complement(749..1969)
                     /locus_tag="VFQ54_02370"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEU0163858.1"
                     /translation="MSLTDRKMNRRVVVATAVAGAGIAVARPAFAQDATPAASPVASP
                     VASPAATPVAGGGLPPLPEGAVVVAQGLWNPGDIALGDDGTLYIAETGVAGGGDSTSE
                     PAAATPNAEGTPPAGAPPLIPPQISKVAPDGTQSVLTTEAGGIGIGLGAGVVYVSTGG
                     GSVGSGFTPLPQENTVSAVDSTTGEVTLLASLGPYEVANNPDGLDVNPNLYGLAVDAS
                     GLLYVADAGGNTIYTVDPTTKTFALFAVVPNLTDLTGATPTAEEQAQQAGPRQPVPTS
                     VVIDAGGNINVVLLGEMWSGPSVLSYAPDGTYSVGISGLSMIVAATIGPDGLLYVSQL
                     SADFTSAQPAPGAVIRIKADGTTENAVEGLFFPSGITFDADGNLYVATNSIISGPDAP
                     LGQVARIDGVATPA"
BASE COUNT          433 a          716 c          801 g          387 t
ORIGIN      
        1 gggccggtga gatagagcgt gatccacgcc gccatatatg gaagatcggg tccgagaaag
       61 accgggcgaa tcgaccatga cgcggtcagg aagaagagga gactcatcgc ggcgccaccg
      121 gccgcgccga tccgcgggag caatcccgtc agcgtcgaga gcccgatcca gagctcggcg
      181 agcgcgacga ggacaccgat cagcacggca ttcggcaacg cgatgtgcct gatcaggaag
      241 gagagcggtg aaccgtccgc cacgtacccg tgcaactgct cgccgatcga ccccgacccg
      301 tccttgttga agaagttggc ggccgtcagc ttctggatcc cggcgtagag gaacatccct
      361 cccaggaaga gacgcagcgg caggatcagt tccggattga tgccggtcgg tcgcgcaggc
      421 tgcggtgaac gggttgaatc ggtgggtggc ggattgaggt tgtcccggga ttgcatgatc
      481 cgctttctct ggtcgcgaag gcgatgaacg cgtcgtccgt tcggcgttgt tgcgggtggc
      541 gggcgatgcc cacgccatcc ggattcccgc gccgggtgaa gactcacagc ggatcgtaat
      601 cggcaaacct gaagggatgt cgcccgtaac gggcaattag cgcgcagatc gtgcattcat
      661 gaacgatgca tgacgaaaac gagaaggagc ggctcgagat tcgagccgct ccttctcgtt
      721 tgatcgggtt ccgaaaccga cggagagatt acgccggcgt ggcgacaccg tcgatgcggg
      781 cgacctggcc gagcggagcg tccgggccgg agatgatcga gttggtggcg acgtagaggt
      841 tgccgtccgc gtcgaaggtg atgccggacg ggaagaagag cccctcgacg gcattctcgg
      901 tggtgccgtc cgccttgatc cggatcaccg cgccgggagc cggctgggcg ctggtgaagt
      961 cggcggagag ctggctcacg tagagcaggc cgtccggtcc gatcgtcgcg gcgacgatca
     1021 tcgagagacc ggaaatgccg acggagtacg tgccatccgg cgcgtaggag aggacggacg
     1081 gtccgctcca catttcaccg agcagcacga cgttgatgtt gccaccggcg tcgatgacga
     1141 cgctcgtcgg aaccggctgg cgcgggcccg cctgctgcgc ctgttcctcg gcggtcggcg
     1201 tcgcgccagt gagatcggtc aggttcggca cgacggcgaa gagcgcaaac gtcttcgtcg
     1261 tcggatcgac cgtgtagatc gtgttgccac cggcgtccgc gacgtagagc agaccgctcg
     1321 cgtcaacggc gagaccgtag aggttcggat tgacgtcgag gccatccggg ttgttggcaa
     1381 cctcatatgg gccaagcgac gccagcaggg tcacttcacc ggtcgtcgaa tcgacggcgc
     1441 tgacggtgtt ctcctgcggc aacggtgtga agccactgcc caccgagccc ccaccggtcg
     1501 aaacgtagac gacaccggcg ccgagaccga tgccgatgcc gccggcttcg gtcgtgagaa
     1561 cggactgcgt tccatccggc gcgaccttgc tgatctgcgg cgggatcagc ggcggtgccc
     1621 cggcaggcgg ggtgccttcc gcgttcggcg tggcggctgc cggttccgag gtgctgtcac
     1681 caccaccggc gacaccggtt tcagcgatgt agagcgtgcc atcatcgccg agggcgatgt
     1741 cgcccgggtt ccagagcccc tgggcgacga cgaccgcgcc ttccggcagc ggcggcaatc
     1801 caccaccggc gaccggagtc gctgccggcg aggcgacggg ggacgctaca ggtgacgcgg
     1861 cgggcgttgc gtcctgcgcg aaggccggac gggcgacggc aatgccagca ccagcaacgg
     1921 ccgtcgcgac gacgacgcga cggttcatct tgcgatcggt caaggacatg tcagattcct
     1981 cccacggtat ggcgcggatc atgattccag cgaacgcgtt caattgtgct gatggggtcc
     2041 ccgtacgcgg ggttctcatg ccgggattcg acaaggactg cgcacttctc tcccagcata
     2101 ccgggttatt tgccacatgg aaagatgttg cctatccgaa tcgatgttcc ccgaccggca
     2161 tcacgtcaaa gggatccgga taacgatcaa ccatcccgct ccctgaatga agacctgcgg
     2221 acgttacggc aagacggccc cgacattgaa aggtggcgcg ttcatcctat cgcttcgcaa
     2281 ccccgacgaa gcgcaagacc tggcgcccgg cgcgcagacg tgtttcctcc ttatggg
//
LOCUS       DASTMD010000153         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2513457, whole genome shotgun sequence.
ACCESSION   DASTMD010000153 DASTMD010000000
VERSION     DASTMD010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2513457"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFQ54_02375"
     CDS             complement(<1..655)
                     /locus_tag="VFQ54_02375"
                     /inference="COORDINATES: protein motif:HMM:NF023354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3445 domain-containing protein"
                     /protein_id="HEU0163859.1"
                     /translation="MNFSTPLHELAWPFDGDEYRYSTNVHSAGSPVPTAAGSWGRSIV
                     EIDAQYAEVIRYRADIVANDPARVQSLPGMSAATWDALVFVLRALADDYPGLMSLDLV
                     DNRYVWKNRLLDVEQTFVFGDDRSLPANPLGFLISQIEEDAFVLTEREGHLHLDAFAS
                     TFAGSWSPVFDLGMTFSELHGPVPRLHGMGMIDRTERFLLSMKPGEIFRRTNWALHVD
                     "
     gene            complement(652..1581)
                     /locus_tag="VFQ54_02380"
     CDS             complement(652..1581)
                     /locus_tag="VFQ54_02380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEU0163860.1"
                     /translation="MRIARINAICAGVREFVLVPVDPSVQLSPYVPGSHIAVEAGAVV
                     NTYSLTDDGYLPDAYRISVRRQDRGGGSDWLHDHVLPGDQLGISKPRSAFAPVLTATS
                     HLLIAAGIGITPILSHLKAARRWNRAFSLVYGVSAERAPHLDEVLALAGDRVTIAEGR
                     VALTAAATDALRQQPIGTHAYACGPAGFLDRFIKDATDLGWPEERLHVEHFDAPGLDP
                     GTPFLAKVAGSDREVAVPAGVSLLEVLENAGIEVPHLCRRGVCGECRTIVHAGVPDHR
                     DLIQTGAERERNDTIYPCVSRSLSPVLELELDA"
     gene            complement(1593..>2249)
                     /locus_tag="VFQ54_02385"
     CDS             complement(1593..>2249)
                     /locus_tag="VFQ54_02385"
                     /inference="COORDINATES: protein motif:HMM:NF041259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylamine monooxygenase subunit DmmA family
                     protein"
                     /protein_id="HEU0163861.1"
                     /translation="RRSAAVSLSGSRALRKVAPMPTSTPIPPDSMDDRTERVSIPSSP
                     KTSVPDWTAGIPRLDERASSWSLLSLGAMPGGAPMIEWKDALTQHGTPFDEVAIDPCD
                     LAVWTANLGSWFPRFVADRVVGWRIAIAGEEFAVLAVRAAALKAGLLECEIIAHVDSR
                     DRFLVYCPDCRTHSLSSRDQDEPMRCQSCQRPLEVHRHVSRRLGSYLGSVSSPETGGH
                     "
BASE COUNT          426 a          684 c          767 g          372 t
ORIGIN      
        1 cgtcgacgtg aagcgcccag ttcgtgcggc ggaagatctc gcccggcttc atgctcagga
       61 ggaagcgttc ggtgcggtcg atcattccca ttccgtgcaa ccgggggacg gggccgtgaa
      121 gctcgctgaa ggtcatgccg agatcgaaga ccggcgacca cgatccggca aaggtcgagg
      181 cgaaggcgtc gagatgaagg tgaccctccc gttcagtgag gacgaaggcg tcctcctcga
      241 tctgggaaat caggaatccc agaggattgg ccgggagcga tcgatcgtcg ccgaagacaa
      301 acgtctgctc gacgtccagt agccggttct tccatacata acgattgtcc acgagatcga
      361 gagacatcag gccgggatag tcgtcggcaa gcgcccggag gacgaagacc aacgcgtccc
      421 aggttgctgc ggacataccc gggagtgatt gcacgcgcgc gggatcgttg gcgacgatat
      481 cagcgcggta gcggatcacc tccgcgtact gggcatcgat ctcgacgatc gagcgacccc
      541 aggaacctgc cgccgtcgga actggcgaac cggcgctgtg gacgttcgtc gaatagcgat
      601 actcgtcgcc gtcgaacggc caggcgagtt cgtggagcgg agtcgagaag ttcatgcgtc
      661 gagctccagt tcgaggacgg gcgagaggga tcgggagacg cagggataga tcgtgtcgtt
      721 gcgctcgcgt tcggcacccg tctggatcag gtcgcggtga tcgggcacac ctgcatggac
      781 gatagtccgg cactctccgc agacaccacg ccggcagagg tgaggaacct cgatcccggc
      841 attctcgaga acctcgagaa gactgactcc cgcgggcacg gcgacctcgc gatcgctccc
      901 cgcgactttc gccaggaacg gcgttccagg atcgaggccc ggtgcgtcga aatgctcgac
      961 atgcagtcgc tcctcgggcc aaccgagatc ggtcgcgtcc ttgatgaatc gatcgaggaa
     1021 cccggcgggt ccgcacgcgt acgcatgggt tccgatcggc tgctgccgta gcgcgtcggt
     1081 cgcggcggca gtcaacgcga cccgcccttc agcgatcgtg acccggtcgc cagcaagggc
     1141 gagcacttcg tcgaggtggg gtgctcgctc ggccgagacg ccatagacga gtgagaaggc
     1201 tcgattccag cggcgcgctg ccttgagatg ggacaggatc ggcgtgattc cgatccccgc
     1261 ggcgatcagc aggtgactcg ttgccgtgag aaccggcgcg aaggccgacc ggggcttcga
     1321 gattccgagc tgatcgccgg gaaggacatg gtcatgcagc cagtcggagc cgccgccccg
     1381 gtcctgcctc cggacggaga tgcgataggc atctggcaga tatccgtcgt cggtcagcga
     1441 gtacgtgttt acgacggctc ctgcctcgac cgcgatgtgg gacccgggca cgtagggaga
     1501 tagctggacg gatgggtcca cgggcacgag gacgaattcg cgtacgcccg cacagatcgc
     1561 attgatcctg gcgatgcgca cagtcgtctc catcagtgtc caccggtctc ggggctggat
     1621 acgctgccga gatagcttcc cagtcgacgc gagacgtggc gatgaacctc cagcgggcgc
     1681 tgacagcttt ggcaccgcat gggctcatcc tgatctcgag atgacaggga atgggtgcgg
     1741 cagtcggggc agtagaccag aaatcgatcg cgggaatcga catgcgcaat gatctcgcat
     1801 tcgagcaagc ccgccttgag cgccgccgcg cgaactgcga ggaccgcgaa ttcctccccg
     1861 gcgatcgcta ttcgccagcc gacgacccga tcggcaacga accgtggaaa ccaggatccg
     1921 aggttcgcgg tccagaccgc gaggtcacat gggtcgatcg ccacctcgtc gaatggcgtg
     1981 ccgtgttggg tcagcgcgtc cttccattcg atcattggcg cgccgccggg catggcgccg
     2041 agggagagga gtgaccacga gctcgcccgc tcgtccagcc tggggattcc ggccgtccag
     2101 tccgggacgg aggttttcgg tgacgatggg atactgacgc gttccgtccg gtcgtccatc
     2161 gaatcaggcg ggatcggcgt ggaggtcggc atcggcgcga cctttcgcaa agcgcgtgaa
     2221 ccggacaacg agaccgccgc gcttcggcg
//
LOCUS       DASTMD010000154         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_371403, whole genome shotgun sequence.
ACCESSION   DASTMD010000154 DASTMD010000000
VERSION     DASTMD010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371403"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..1714)
                     /locus_tag="VFQ54_02390"
     CDS             complement(245..1714)
                     /locus_tag="VFQ54_02390"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEU0163862.1"
                     /translation="MRFAIGYFAHETNTFCPGLTELADFEASLWEVGDEILVNHRGVR
                     DDLGGMIAAGERLGIDLVATLATTTQPSATVSRHAYETIRDEMFAHLTAAGAVDAIVL
                     TLHGAGSAEDMDDMEGVFLKELRDLVGTEIPIVVSLDLHGNTTEAMLEHTNALLYCHE
                     YPHIDMYERGDEAVELAVKIVRGEVKPVMHLEKLPAIIPPSTTFTGPAKIVNDYCLAW
                     EQKPGIIDCNFTHGFPDTDVSVIVTSILVTTDDDPALAASVARDVAAFTLGMIGEFLQ
                     DLPNAEDAVAQALAATELPVVIAEVSDNPGGGAPGSGTHLLRALLAANEPDTCFGFVW
                     DPETAAQAHAAGPGATIQVRLGGFTDELHGAPIETEAYVKLVTDGRFILTTPMGAGMR
                     EDYGKMARLVIGNVDVIVGTVRSQTFDAELFLLHGIDVRRMRIVALKSQQHFRAGFLD
                     VAGTIIRADTPGFTTSNLLNLPYRRITRPIWPLDPLPVG"
     gene            complement(1828..>2409)
                     /locus_tag="VFQ54_02395"
     CDS             complement(1828..>2409)
                     /locus_tag="VFQ54_02395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0163863.1"
                     /translation="VVVAAGIYTPRLLAPLLAPIGLTLPISVTMVAAIQSIPLPRMLS
                     QVIGVASGRVAGRQEVSGRFRMTGTSMPWSEDGFHSAETTRPVFGQIQDTIERAIRVV
                     PALAGARLVNTWGGLIDQSPDAIPVIDRPAAIDGLVVAAGFSGHGFCLGPITGEILAD
                     LATAGTTPHPIDAFRLDRFPAQATPPEAVHLHG"
BASE COUNT          425 a          748 c          828 g          408 t
ORIGIN      
        1 cgggatgacg tgtcttgcga ggcgttgtat ggaaaagatg gagattccac gaccagggcg
       61 atcaccagag cctgtcccag cagctgcacc ggagcgcagc cgagggaatc gcccctacgg
      121 aatggcgtaa gggaacggaa agtcgtaggg gtcagcctgg tgctgacccg cggccgcagg
      181 ccgccctcgt cgctcgcagg gtcaacccac gccttcgacc atggctcgct gcgcgaatcc
      241 cggatcagcc gacaggcagc ggatcgagcg gccagatcgg acgggtgatt cgccggtagg
      301 ggaggttcag caggttgctc gtcgtgaaac ctggggtgtc ggcgcggatg atcgttcccg
      361 ccacatccag gaatccggcg cggaaatgct gctgcgactt caaggcgacg attcgcatcc
      421 ggcggacgtc gatcccatga aggaggaaga gctcggcgtc gaaggtctgc gagcgcaccg
      481 tgccgacgat gacgtcgaca ttgccgatca cgaggcgcgc catcttgccg tagtcctcgc
      541 gcatcccggc gcccatcggg gtcgtcagga tgaagcgacc gtcggtgacg agcttgacat
      601 acgcctcggt ctcgatcggg gcgccgtgga gttcatccgt gaagccgccg agcctgacct
      661 ggatcgtcgc gccaggtccg gcggcgtggg cctgggcggc ggtctccgga tcccagacga
      721 agccgaagca ggtatccggt tcgttcgccg cgagaagcgc ccgcaacaga tgcgttccgc
      781 ttcccggcgc gccgccacca gggttgtccg agacctcggc gatcacgact ggcagttccg
      841 tggcggcgag cgcctgggcg acggcgtcct cggcgttcgg aagatcctgc aggaactccc
      901 cgatcatccc cagcgtgaag gcggcgacat ccctggcgac gctcgcggcc agcgcgggat
      961 cgtcgtcggt cgtgacgaga atcgaggtga cgatcacgga gacatcggta tccgggaagc
     1021 cgtgggtgaa gttgcagtcg atgatccccg gtttctgttc ccacgcgagg cagtagtcgt
     1081 tgacgatctt tgccggtccc gtgaaggtcg ttgaaggcgg gatgatcgcc ggcagcttct
     1141 cgaggtgcat taccggcttg acctcgccgc ggacgatctt caccgccagc tcgaccgcct
     1201 cgtcgccgcg ctcgtacatg tcgatgtgcg ggtactcgtg acagtagagc aacgcattcg
     1261 tgtgctcgag catcgcctcg gtcgtgttgc cgtggagatc gagcgagacg acgatcggga
     1321 tctcggtgcc gacgagatcg cggagttcct tgaggaagac accttccatg tcatccatgt
     1381 cctcggcaga ccccgctccg tgcagcgtga gcacgatcgc atcgacggcg cccgcagcgg
     1441 taaggtgggc gaacatttca tcgcggatcg tttcgtaggc gtgacgggag acggtcgccg
     1501 acggttgcgt cgtcgtcgcg agcgtggcga ccaggtcgat gccgagacgc tcaccggcgg
     1561 cgatcattcc gccgagatcg tcacgcacgc cgcggtggtt gacgagaatc tcgtcgccga
     1621 cctcccagag cgatgcctcg aaatccgcga gttcggtcag ccccgggcag aaggtgttcg
     1681 tctcgtgggc gaaatagccg atcgcaaagc gcatcgttcg tctcctcgaa gggtggatgg
     1741 tcggcagaag tcaactgcag gcgatcctat cgcggaacct atccgagggg agaatgttct
     1801 cgatcgcgtc atcgaaggtc ggtgattcta tccgtgcagg tgcacagctt ccggcggcgt
     1861 cgcttgtgcc gggaagcgat cgaggcggaa cgcgtcgatc ggatggggtg tcgtcccggc
     1921 ggtggcgaga tcggcgagga tctctccggt gatcggtccg aggcagaatc cgtggccgct
     1981 gaatcccgcc gccacgacga gcccatcaat cgcggccggc cggtcgatga cgggaatcgc
     2041 atccggcgac tggtcgatca gtcctcccca ggtattcacg aggcgagcac ccgcgagcgc
     2101 gggcacgacc cggatcgccc gctcgatggt gtcctggatc tgtccgaaga cggggcgggt
     2161 cgtctcggcg gaatggaatc cgtcctcact ccacggcatg gatgtccccg tcatccggaa
     2221 gcgccccgaa acctcctggc gaccggcgac gcgaccagat gcgacgccga tcacctgcga
     2281 aagcatccgc ggcagcggga tgctctggat cgcggcgacc atcgtcacgg agatgggaag
     2341 ggtaagcccg atcggcgcga gcagcggcgc gagcagcctc ggcgtataga ttcccgccgc
     2401 gacgacgac
//
LOCUS       DASTMD010000155         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_995953, whole genome shotgun sequence.
ACCESSION   DASTMD010000155 DASTMD010000000
VERSION     DASTMD010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995953"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFQ54_02400"
     CDS             complement(<1..321)
                     /locus_tag="VFQ54_02400"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU0163864.1"
                     /translation="MNPEYSGLLGLVGDIKIHEANLRQRRDQNAPFDQAIAALESHGL
                     AIGNLEMPLSTRGYRVPKHSNIRSNPEVIAEVLTMGIQAVSLANNHMMDYGPDALIDT
                     LATCA"
     gene            complement(321..1235)
                     /locus_tag="VFQ54_02405"
     CDS             complement(321..1235)
                     /locus_tag="VFQ54_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0163865.1"
                     /translation="MTTLSLAATTPPDIREATKHSSRSFWRIAWRRFRRNRIALASAI
                     FLIAICAVALFAPAITPHNPNAVNILMPHGKPSSDHLLGTDESGRDVFSRLLVGARVS
                     IAVGITSMIISITIGTLVGSLAGFSGRWIDALLMRITDGMMAIPYFFLVLIVVAVFGT
                     SFRNIVLVIGVTSWIVVARIVRSEVLRYRNYEFVTAARALGATNARQLFRHVLPHTLP
                     SIIVAATLGVANAILLESALSYLGLGIQPPQASWGNMLSNSQAYLFDNPLLPLYPGTL
                     ILVTVLAFNFLGDALRDALDPQYKEGSH"
     gene            complement(1248..>2151)
                     /locus_tag="VFQ54_02410"
     CDS             complement(1248..>2151)
                     /locus_tag="VFQ54_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0163866.1"
                     /translation="LFIVSMVTFALIHSAPGGPAILANPDLSRDQIQRMSNQLGLNDP
                     LPVQYGRWISNVFHGNFGVSYNTIEPVGSLIGNRLPNTLLLAGVALLISVTIAIPLGI
                     ICAMKRNSILDRLVTSFTFLGVSIPVFWLGIMLIVLFSVRYRWLPAGGMSTVGNGFSL
                     HDRIDHMILPVFVLVIGNLAELTRYTRSSMITVLGEEYIRTARAKGLTQKSVITGHAL
                     RNALIPVITIIGVLLPRAVSGAAITEAVFSWPGMGSLAVEAATTRDYPVVLATTLTVA
                     VVVVLSSLLTDLAYGFLDPRIRLG"
BASE COUNT          421 a          592 c          769 g          369 t
ORIGIN      
        1 ggcgcaggtc gccagggtgt cgatgagggc gtccgggccg tagtccatca tgtgattgtt
       61 ggcgagggag acggcctgga tccccatggt gaggacttcg gcgatcacct ccgggttcga
      121 ccggatattg gaatgcttgg ggacccgata gcctctcgtc gagagcggca tctcgagatt
      181 cccgatcgcc agcccgtgcg attcgagtgc ggcgattgcc tgatcgaatg gcgcgttctg
      241 atcgcgtcgt tggcggaggt tcgcctcgtg gatcttgatg tcgcccacga gcccgaggag
      301 accggagtat tccgggttca tcagtggctt ccttccttat attggggatc gagggcatcc
      361 cggagggcat cgccaaggaa gttgaaggcg agcaccgtga cgaggatgag cgttcccggg
      421 tagagcggaa gcaggggatt gtcgaagaga taggcctgcg agttgctgag catgttgccc
      481 cagctcgcct gcggtggctg gatgccgaga ccgagatagc tcaatgcgga ttcgagcagg
      541 atcgcgtttg ccaccccgag cgtcgcggcg acgatgatcg aggggagcgt gtgcggcagc
      601 acatgccgga agagctggcg cgcgttggtc gcgcccagtg cgcgcgcggc ggtaacgaat
      661 tcgtagttgc gataccgcag gacctcactg cggacgatgc gggcgaccac aatccagctc
      721 gtgacgccga tgacgagcac gatgttgcgg aacgatgtcc cgaagaccgc gacgacgatc
      781 agcacgagga agaagtaggg gatcgccatc atgccgtcgg tgatccgcat caggagcgca
      841 tcgatccagc gaccgctgaa tccggcgaga ctcccgacga gtgtgccgat cgtgatcgag
      901 atgatcatcg acgtgatgcc gacggcgatg gaaacccgcg cgccgacgag aagacgggag
      961 aagacgtcgc gcccgctctc gtccgttccg aggagatggt cggacgacgg cttgccgtgc
     1021 ggcatcagga tgttgaccgc gttcggattg tgcggcgtga ttgccggtgc gaagagcgcg
     1081 accgcacaga tggcgatcag gaagatcgca ctcgcgaggg cgatccggtt gcgccggaag
     1141 cgtcgccacg cgatccgcca gaatgaacgc gaggagtgct tcgtcgcctc gcggatgtcg
     1201 ggcggtgtcg tcgcggcgag ggagagtgtc gtcatggtgg agcccatcta tccgagacgg
     1261 atgcgggggt cgagaaaccc gtaggcgaga tcggtcaaca ggctgctgag gacaacgacg
     1321 accgcaacgg tcagcgtcgt cgcgaggacg acgggatagt cgcgggtggt ggcggcttcg
     1381 accgcgagac tgcccatgcc gggccaggag aagacggcct ccgtgatcgc ggcaccgctg
     1441 acggcgcgcg gaaggaggac gccgatgatc gtgatgaccg ggatcaacgc gttgcgcagg
     1501 gcatgtccgg tgatgacgct cttctgggtg agcccctttg cccgcgcggt gcggatgtat
     1561 tcctccccga ggaccgtgat catgctcgac cgggtgtagc gcgtcagttc ggcgagattg
     1621 ccgatgacga gcacgaagac cggcaggatc atatggtcga tgcggtcatg gagcgaaaac
     1681 ccgttgccga cggtgctcat gcctcctgcc gggagccagc gataccggac cgagaagagc
     1741 acgatgagca tgattcccag ccagaagacc ggaatcgaaa cgccgaggaa ggtgaagctc
     1801 gtgaccagcc gatcgaggat ggagttgcgc ttcatggcgc agatgattcc cagcggaatc
     1861 gcgattgtca ccgagatgag cagcgcgacc ccggcgagca gcagcgtgtt cggcaggcgg
     1921 ttgccgatga ggctcccgac gggttcgatc gtgttgtagc tgacgccgaa gtttccatgg
     1981 aaaacgttgg aaatccagcg tccgtactgc accgggagcg gatcgttcag gccgagttga
     2041 ttggacatcc gctggatctg gtcgcggctg agatccgggt tcgccagaat cgccggtcca
     2101 ccgggtgccg agtgaatcag cgcgaaagtc accatgctca cgatgaagag c
//
LOCUS       DASTMD010000156         1579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1800289, whole genome shotgun sequence.
ACCESSION   DASTMD010000156 DASTMD010000000
VERSION     DASTMD010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800289"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFQ54_02415"
     CDS             complement(<1..644)
                     /locus_tag="VFQ54_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0163867.1"
                     /translation="MKRYTWKNLTWEIWMIIVALIFCLPFYILISVALKPSSDVFTTS
                     PLAFPTTPDFSNFSQAWAAPAGGGLGPALVSSLIITVGAVVGLIIFGSLCAYALARRP
                     GRLSNALYFLFVLGIIIPFQLSVIPLYVAFRQAGLIGTYLGMILLWIGIGSPLSVFLY
                     TGFIRSLPKDYEEAARLDGAGIFRIYWFVVFPLLRPITGTVAILLGLFIWNDFFV"
     gene            complement(641..>1579)
                     /locus_tag="VFQ54_02420"
     CDS             complement(641..>1579)
                     /locus_tag="VFQ54_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0163868.1"
                     /translation="MTTTSSTTTAPGPRVRRRRIYLGSVKVPWLFAVPAIVIILLLRY
                     IPSIAGGAYAFTDWNGSSLSANWAGVSNFQKIFADPAVSGALWHTLVLAVLFVIIVNI
                     VGLALALSLRTTLKTKSVLRALFFLPFALSQLATAYIWQFVFAYDGPLNVVLKAVGLG
                     GSVKSWLSDPQFALIAILVVVVWQYTGLTMIIYLAGLEGISEEITDAAAVDGASFLQR
                     FRWVTLPLLAPAMTIAISLTLIFGLGVFDQILGLTGGGPVDSTQTIATQVWQQTFVYG
                     NFAQGSALALLFAALVSVLALLQVFILRIRERRLEK"
BASE COUNT          363 a          457 c          524 g          235 t
ORIGIN      
        1 acgaagaagt cgttccagat gaacagaccc agcaggatcg ccaccgtccc agtgatcggc
       61 cggaggagag ggaacacgac gaaccagtag atgcggaaga tgcccgcgcc gtcgagtcgc
      121 gccgcctcct cgtaatcctt cgggagtgac cggatgaagc ccgtatacag gaagaccgaa
      181 agcggggaac cgatgccgat ccacagcagg atcatcccga ggtaggtccc gatcagcccc
      241 gcttgccgga acgcgacata caacgggatg acgctcaact ggaaggggat gatgatcccc
      301 aggacgaaga ggaagtacag ggcgttgctg agacgtccgg gccgcctcgc cagggcgtac
      361 gcgcagagcg atccgaaaat gatcagtccg acgacggctc cgacggtgat gatcaggctg
      421 ctgacgagcg ccggacccag cccaccaccg gccggggccg cccatgcctg gctgaagttc
      481 gagaagtcgg gtgttgtcgg gaacgccaaa ggactcgtgg tgaagacatc gctggatggc
      541 ttgagggcca cgctgatgag gatgtagaag gggaggcaga agatcagcgc aacgatgatc
      601 atccagattt cccaggtgag gttcttccag gtgtatcgct tcacttctcg agacgccttt
      661 cacgaatgcg caggatgaat acctgcagca gagccagaac cgagaccagc gctgcgaaga
      721 gcagggccag agctgatccc tgcgcgaagt ttccgtagac gaacgtctgt tgccagacct
      781 gggtcgcgat cgtctgcgtc gagtcgacgg gacctccgcc ggtcaggccg aggatctggt
      841 cgaagacgcc gagaccgaag atcagggtca aactgatcgc gatcgtcatc gccggcgcga
      901 gcagcggcag agtcacccag cggaaacgct gaaggaacga ggcgccatcc acggcggccg
      961 cgtcggtgat ctcttccgag atcccctcga gacctgccag gtagatgatc atcgtcaggc
     1021 ccgtgtactg ccataccacg accaccagaa tggcgatgag cgcgaactgg gggtcggaca
     1081 accacgactt gacggagccg ccgagcccga cggctttcag aacgacgttc aaggggccgt
     1141 cgtaggcgaa gacgaactgc cagatgtacg ccgtcgccaa ctggctcaga gcgaagggca
     1201 ggaagaacaa ggcccgcaga acgctcttgg tcttgagggt cgtgcgcaag ctgagcgcga
     1261 gggccaaccc gacgatgttc acgatgatca cgaagaggac cgcgagcacc agggtgtgcc
     1321 acagggcgcc gctgaccgcg ggatccgcga agatcttctg gaagttggac actcctgccc
     1381 agttcgcgga gaggctactg ccgttccagt cggtgaacgc gtatgcgccg ccggcgatac
     1441 tcggaatgta gcggagcagc aagatgatca cgatcgccgg cacggcgaag agccagggaa
     1501 ccttcacgga tccgaggtag atgcgtcgac ggcgcacgcg cggacccggc gccgttgttg
     1561 tagaagaggt ggttgtcat
//
LOCUS       DASTMD010000157         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3138797, whole genome shotgun sequence.
ACCESSION   DASTMD010000157 DASTMD010000000
VERSION     DASTMD010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3138797"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            793..2700
                     /locus_tag="VFQ54_02425"
     CDS             793..2700
                     /locus_tag="VFQ54_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0163869.1"
                     /translation="MRPRPARGGGPGRGRPGRSRPGRSRRLNPEDDVPVTPETIRRFP
                     WRRLWRYTRDVRGRMLISAIALLVSSGTMIAFPALIGDGIGHVIERGSRSDLNLLVLT
                     LLGVFFAGAVANYVNSYMLSFTGQTINRRIREDVFGKIVGLDAPFHATHRSGELASRL
                     SNDTTTLQSIFTGMLPSLLSGLLGLVGTIVVMIGLNPQLTLFMFISVPAIMIVAITIG
                     RRLERLATAGQDQLAAVNAQATEVFTNLPIVKSFTAETYETNRYENALSELFAINIRN
                     ARYSAANVALITFLAFSGIAGVVWFAGRQVIDGQSSIGTITTFLIYGIQIAMNFGQLS
                     SLFTQFKQASGASLRVFEILDSDLTVPEPEHPRPLAGISPTIAFDDVSFRYGADLVLD
                     DVSFTVGAGETVALVGPSGAGKSTIVELLQRSYLPEAGTIRLGGIDISEVTSHDVRSE
                     MAVVSQMAPLFKGTVRANIAYGKQEATEAEIVAAAKAAQAHEFIERLPEGYDTELGDN
                     GAGISGGQRQRLAIARAMVKRAPFLLLDEATSALDNTNEALVQQALNELMRDTTTLVV
                     AHRLTTIEGADRIVVLDEGRVIEEGTHIALLAKGGFYADLHARTIREHGEGEDRAIID
                     ERSPEVANIIR"
BASE COUNT          586 a         1063 c          966 g          616 t
ORIGIN      
        1 gtcaaacgcg atcgcggttg cctcggccaa ctcatcaaca tctggatcgt gatcgaagaa
       61 cacgtcggtc gtaagaatca tggtcatcgc ttcgatcacg cattcgccag atacgctgtg
      121 cctcgtcgcg gtcctcgtca gatattttca tttgcatccg ctcgaattcg gaccgctcat
      181 taatgccatt gtatcgcgca taggccttca atgcggcaac cacggggcga gtgactgggt
      241 cggtgaagcc atccccgtgg agtggaaaca gcactcctgt cggcttttgc tgataggtgc
      301 gtccgttcac ggtaaaacgg tcacccgctc ggtgtgccag cccctcattg attgcgtgaa
      361 tatcggcctg aacgtcaacg cctggttcta tgaacgtcgt caacgtatcc gcgagcatcg
      421 tcgattttat gcgcgtgatg aaatgtcgat cagcaggcat tctcgcgagc gttcgaggtc
      481 gattgtccgg tgaacgtccg cctgattccg tcacctttgg catgccttgc gagatattcg
      541 gatcgcacac atcgtctcgg gcccgaatga tcctaccatc tcgagcaact cctgcggtcg
      601 tccatcgccc ttccttctca ggaagcgtgg atcgactgcc gggtcgccca cccagtcggg
      661 tgatgcagag cacatggctc atcctgtaca acttggcaac tcgctcgcga ttcccgcgta
      721 tacacacgag gatcgccatc gctgcccggg caacgacgcc ccatcacctc catcgaaagg
      781 aacgcgagaa ccatgcgacc aagaccagca cgcggtggcg gcccgggacg tggccgcccc
      841 ggacgctccc gccccggacg ctcccgccgc ctcaatcccg aggacgacgt tcccgtcacg
      901 ccggaaacga tccgccgctt tccctggcgt cgcctctggc gttacacccg cgatgtccgc
      961 ggtcgcatgc tcatctcggc gatcgcgctc ctcgtcagca gcggcacgat gatcgccttt
     1021 cccgccctca tcggggacgg tatcgggcac gtgatcgagc gtggcagtcg ctccgatctc
     1081 aatctcctcg tcctgaccct gctcggcgtc ttcttcgccg gcgcggtcgc gaactacgtc
     1141 aattcctaca tgctctcgtt caccgggcag acgatcaacc ggcgaattcg cgaggatgtc
     1201 ttcggaaaga tcgtcggcct cgacgccccg tttcacgcga cccaccggag tggagagctc
     1261 gcttcccgcc tctcgaacga cacgacgacg ctccagagca tcttcaccgg gatgctgccg
     1321 tcgctcctga gcggattgct cggtctggtc gggacgatcg tcgtcatgat cggactcaat
     1381 ccgcagctga cgctcttcat gttcatctcc gtgcccgcga tcatgatcgt cgcgatcacg
     1441 atcggtcggc ggctcgagcg cctggcgacc gccgggcagg atcaactcgc cgctgtcaac
     1501 gcgcaggcga ctgaggtctt cacgaatctc ccgatcgtca agtcgttcac cgccgagacc
     1561 tatgagacga atcgctacga gaacgcactc agcgaactct tcgcgatcaa catccgcaac
     1621 gcccgctact cggcggcgaa tgtcgcgctg atcacgttcc tcgccttcag cgggatcgcg
     1681 ggtgtcgtct ggttcgccgg tcgccaggtg atcgatgggc agtcgtcgat cggcacgatc
     1741 acgacctttc tcatctacgg catccagatc gcgatgaact tcgggcagct gagctcgctc
     1801 ttcacccagt tcaagcaggc gagcggcgcc tcgcttcgcg tcttcgagat tctcgacagc
     1861 gatcttacgg tccccgagcc ggagcatccg cgtccgctgg ccggaatctc gccgaccatc
     1921 gccttcgacg atgtgagctt ccgctacggc gcggatctcg tcctcgacga tgtctcgttc
     1981 acggtcgggg cgggggagac ggtcgccctg gtcggtccga gtggcgccgg gaagtcgacg
     2041 atcgtcgagc tgctccagcg ttcctacttg ccggaggccg ggacgatccg gcttggtggg
     2101 atcgatatct ccgaggtgac atcacacgat gtccggagcg agatggcggt ggtgtcgcag
     2161 atggcgccgc tcttcaaggg aacggtccgc gcgaacatcg cctatggcaa gcaggaggcg
     2221 acggaagccg agatcgtcgc cgcggcgaag gcggcccagg cccacgagtt catcgagcgc
     2281 ctgccggagg gctacgacac cgagctcggg gataatggcg ccgggatcag tggtggccag
     2341 cggcagcggc tcgcgatcgc acgggcgatg gtgaagcgcg cgcccttcct gctcctcgac
     2401 gaggcaacga gcgcgctcga caacacgaac gaggcgctgg tccagcaggc gctcaacgag
     2461 ttgatgcgcg acacgacgac gctggtcgtc gcccaccgcc tgacgacgat cgagggcgcg
     2521 gaccggatcg tggtgctcga tgaggggcgc gtgatcgaag aggggacgca catcgcgttg
     2581 ctggcgaagg gcggcttcta cgccgatctc cacgcccgaa cgatccgcga gcacggcgag
     2641 ggcgaagacc gcgcgatcat cgacgagcga tcgccggagg tggcgaacat catccgttga
     2701 cgacccgcat tcgtagcggg ccacctcgtc ccgatatgat gcctgcatct atggagtttg
     2761 ccgatccccc aacctcggat gaacagtaga cgcggtgctt gtcatcgcct ggggtcggca
     2821 tagccgatcc cgtgcccgct ggcgaacgat gcggaccggc ggcgcgttcc cgtttgcggc
     2881 ggggtataaa accccgccct accaaccccg gctccaccaa tcgtgtcgat agctccggat
     2941 tgtgctgtgg caagaacggc cttgtgtcgg cccgcgaacg gaatcgttgg cacaacgcgt
     3001 atcagtttga tacctcgaca cggcactacg cgggcacgga caattgcctg atcggacgcc
     3061 gtaccgtgac tatgcattga tggagatcgc cgtgcctacc cttccacccc cagaaagttc
     3121 cgaaccagac gggatctcga tcgaaagtgc ccgcaacctg acgatcatct acagcttgct
     3181 gatcgggctc gcctccgtcc tcgcgatcat cgcgggcaag gacaactggg c
//
LOCUS       DASTMD010000158         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2514034, whole genome shotgun sequence.
ACCESSION   DASTMD010000158 DASTMD010000000
VERSION     DASTMD010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2514034"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            987..2069
                     /locus_tag="VFQ54_02430"
     CDS             987..2069
                     /locus_tag="VFQ54_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU0163870.1"
                     /translation="MRPAGWGIQRATPRRVAVATLAVLVLMIGAVGTSLADDQYRYVV
                     QSGDTLQSVATEFGVDPDAIVASSYMPDGTTLDPGQVIIIPEQGQSPSDAAAMASERE
                     GTSPYASAAYTVQWGDTVDSIASQFGVDPGTLADLNGISDPTSIRSGDRLVIPGSADG
                     SPSSNAGAASATTTSDSSGLQVPNVSTHVQERNLSCEYASVYIATAAFGNPIPEDVMI
                     NNIPVTLNPHDGFRGNIDGPWGNDTDYGIYPEALTSTLSDYGFASDIFYGEGDTTQLK
                     AELDAGHPVLTWIAMQGNTREQFNDDGTYVVVPGDHVMVAYGYDDSGIYLSDPAHGEY
                     TYYTWDDFVYMWKIMDGMSMAVYPAS"
BASE COUNT          429 a          706 c          747 g          420 t
ORIGIN      
        1 gaggaacgcg gacggaacga tcgccgggca cgcgtcgcgc gccgggattg cttccgggga
       61 atgatcggcc tggatggcga tggcaatgaa ctgagccacg atcgcgtcct gttccggtcg
      121 agcatcgcga atcgcgcgtg cgatcgcagg acatgccgct tctgacctca tgatattcgg
      181 caccccgcaa ccggcgcaac ccccatcgcg gggacatcgt acgtcgattc ttcccgcctg
      241 ccgttcgttc atcaggaaac gacgcattgc gtgcgggaca taacgatgga ttccgcgagt
      301 tctttgcgga cgccgcgaca tcaacgctgg tccggcgctt gcgcatcaag catcagcgcc
      361 gggttgccag tctgtcacaa ggcgagtatt ctgcttgtgt acgtacaccc atggacacac
      421 ccgtgtggat cacggggcac tcgttgaaca gagacggcat cccgaggctc gattcccgcc
      481 gcttgcccgg gagcggaggg actcttgata tggcacggca tcgtgactcg tggctgcaga
      541 acgccgattt cgatccgtcg catgtctggc gcaaccacgg tgaatcgaac ggatgcatcg
      601 tcctcggatc cggagaggtg gctgaccgtc tcaacgatcg cccggggatc tggagcgagg
      661 agcgcgcgtt accgcggcat cgggctgatg atgagttcgt gcccggagaa ccggggcacc
      721 agatttcgac gtatcgagag tgcggtctcg tcagcatgta tgcttctcgt agccgccgtt
      781 cgatgcgacc ttcgggatcg tcatcgatcg ataccgaaac cgggcggccc ccggtttcgt
      841 tcgggagacg gagacaggcg cattacgatg cgcttcatcc gcagcgtcgg cgtgaataca
      901 gacgaattcg ttggggggga ttggggatgc ttgtccgttc gggacgagaa gcggagacgt
      961 ttgcggcaag cggacgggag cggaagatgc gcccggcggg atggggcatc cagagggcga
     1021 caccacggcg cgtggccgtc gcgacgctgg ccgtgcttgt cctgatgatc ggcgcggtgg
     1081 gcaccagtct cgccgacgat cagtaccggt atgtcgtgca gtccggagac acgcttcagt
     1141 ccgtggcgac cgaattcggc gtcgatccgg atgcaatcgt ggcttcgagc tacatgccgg
     1201 atgggacgac gctcgatccc gggcaggtca tcatcatccc tgagcagggg caatccccgt
     1261 cggatgcggc agcgatggcc tcggagcgcg agggcaccag cccgtatgcg tccgctgcct
     1321 acacggtgca gtggggggat acggtcgaca gcatcgcgtc gcagttcggc gtcgatccgg
     1381 gaacgctggc cgatctcaac ggcatctccg atccgacatc gatcaggtct ggcgatcgtc
     1441 tcgtgatccc ggggtctgcc gatggctccc cgtcgtcgaa cgcgggagct gcgtcagcga
     1501 ccaccacgtc cgattcctcg gggctccagg tgccgaatgt ctcgacccac gtccaggaac
     1561 gaaacctcag ctgcgagtac gcctcggtgt atatcgcgac cgctgccttc ggaaacccga
     1621 tccccgagga tgtgatgatc aacaacatcc cggtcacgct gaatccacat gacggctttc
     1681 gcggcaacat cgatgggccc tgggggaacg acaccgacta cggcatctac ccggaggcgc
     1741 tcacctcgac gctcagcgac tatggtttcg cgtccgacat cttctacggc gagggtgata
     1801 ccacccagct gaaggccgag ctcgatgccg gccatccggt cctgacctgg atcgcgatgc
     1861 agggcaacac ccgcgagcag ttcaatgatg acggaaccta cgtcgtcgtt ccgggcgatc
     1921 acgtgatggt ggcctacggc tacgacgaca gcgggatcta cctctcggat ccggcccatg
     1981 gtgaatacac ctattacacg tgggacgatt tcgtctacat gtggaagatc atggacggca
     2041 tgtcgatggc ggtctacccg gccagctgat cgccgagctt ccaaccgact ttgagcaaac
     2101 gaccccgccg gcacccgccg gcggggtcgt tgtccgtgtg aactcgcctg cgatcgatcc
     2161 acgtggcggg tcaccccgta cgggtgatgc atagtcaggg tttgatcatg aacggacacg
     2221 gcgtcggcga cgatggaagg tgcccactcc ggggcgcggc tgaccagtgg aaaggcccca
     2281 gccatggatc ccgacgtcga ca
//
LOCUS       DASTMD010000159         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2514155, whole genome shotgun sequence.
ACCESSION   DASTMD010000159 DASTMD010000000
VERSION     DASTMD010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2514155"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFQ54_02435"
     CDS             complement(<1..644)
                     /locus_tag="VFQ54_02435"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU0163871.1"
                     /translation="MDALFDRPIDDSGWARLRRHGRLWRRFLMLAVVREAQFRAHFVA
                     TVVVGLAQVVVALIPILLIFNFTSAVHGWSQADLIVLLGIHQLVSALLAIAIAPNVTR
                     MTEYITHGDLDLMLIRPVDAQFYVTFRWLQPAEAFNVAIGIGLVVVGLARAGEMPGLV
                     AIGQGVLLLLCGVILLTCAWSAIAFCIFWVSSLHLGMTIFYDVMDAGRYPVVFYP"
     gene            complement(648..1457)
                     /locus_tag="VFQ54_02440"
     CDS             complement(648..1457)
                     /locus_tag="VFQ54_02440"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU0163872.1"
                     /translation="MTVPQSLRLIRRMTAVNMAVTLAYRLDFAFYMLSAIISPIISVL
                     VWRAAIASGADLPVSASYLTTYFVLFAVVSVATGVWLATFLADSIRSGNISIWLARPG
                     SPLYEMVANNLAEKAFKAVVLAPMILIFGWFLSDSLALGVAPWRWLLFVLSTVLAAVI
                     AFSLDVMVASLAFWIDDVGGVVRGLGIVSGVLAGRIVPLALMPEWSRGFVDVQPFRFT
                     LSFPLEMIVGDLSGREVAAGFGLQAIYAAAFILAARWIWQRGQLVYSAVGA"
     gene            complement(1454..2521)
                     /locus_tag="VFQ54_02445"
     CDS             complement(1454..2521)
                     /locus_tag="VFQ54_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0163873.1"
                     /translation="MMQTIPGTFVPAESSTLRSAEASTIAIDAVGLTKTYTFHRQSPG
                     LAGAFRSVFRRQTETRLAVDRVSFRIARGEIVGLLGPNGAGKTTTLKMLTGLLHPSDG
                     RIDVLGFQPFDRKTEYLRRIALVMGQKTMLWWDVPAAESFLLHKEMYGLPDAVFRDSV
                     DELATMLDVEDLLNVQVRKLSLGERMKMELLAALLHRPEVLFLDEPTIGLDVVAKARV
                     RTFLAEVNQRRGTTILITSHDMDDIEALCSRVMIVNHGRIAYDGGLVDLVQSVQPRKL
                     IRATFAAPVDASALGGDVTRIPVEDDDGRVVALEVPRDGLSDLLELLPRLGPMIDLSV
                     ADADVEEIIRSLFAGETVVAR"
BASE COUNT          618 a          968 c          951 g          436 t
ORIGIN      
        1 gggtagaaga cgaccgggta tcgccccgca tccatcacgt cgtagaagat cgtcattcca
       61 aggtgcagcg aactcaccca gaagatgcag aaggcaatcg ccgaccacgc gcatgtcagc
      121 aggatgacgc cacagagcag cagcagcaca ccctgcccga tcgcgacgag acccggcatc
      181 tcgccagccc tcgcgagtcc aaccacgacc agcccgatcc cgatcgcgac gttgaaggct
      241 tccgccggct gcagccagcg gaacgtcacg tagaactggg catcgaccgg ccgaatcagc
      301 atcagatcga ggtcgccgtg cgtgatgtac tcggtcatcc gggtcacgtt cggcgcgatc
      361 gcgatcgcga gcagcgccga cacgagctga tggatgccga ggaggacgat caggtccgcc
      421 tgcgaccagc cgtgaaccgc gctcgtgaag ttgaagatga gcaggatcgg aatcagcgcg
      481 acgacgacct gcgcgagccc gaccacaacg gtcgccacga agtgcgcgcg aaactgcgcc
      541 tcccggacga cggcgagcat gaggaagcgt cgccacaggc ggccatgccg ccgcagacgc
      601 gcccatcccg aatcgtcaat tggtcgatcg aagagtgcgt ccatcagtta cgcccccacc
      661 gcgctgtaca cgagttgacc gcgctgccaa atccagcggg cggcgaggat gaacgcggcc
      721 gcgtagatcg cctgcagccc gaacccggcc gcgacctcgc gcccgctcag gtcgccaacg
      781 atcatctcga gcggaaacga gagcgtgaag cgaaagggct ggacatcgac gaatccccgc
      841 gaccattcag gcatcagcgc caggggcacg atgcgccccg cgagcacgcc ggagacgatg
      901 ccgagaccac ggacgacccc gccgacgtcg tcgatccaga aggcgagcga ggccaccatc
      961 acgtcgagcg agaaggcgat caccgcggcg agcaccgtac tcagcacgaa gagcagccat
     1021 cgccacgggg cgacgccaag cgccagcgag tcactcagga accagccgaa gatgaggatc
     1081 atcggcgcga gcaccaccgc cttgaacgcc ttctccgcca gattgttcgc gaccatctcg
     1141 tagagcggtg accctgggcg tgcgagccag atcgagatgt tgccactccg gatcgaatcc
     1201 gcgaggaagg tggcgagcca gacacccgtc gccaccgaga cgaccgcgaa gagcacgaaa
     1261 taggtcgtca ggtaggaggc ggagaccgga agatcggcgc cgctggcgat cgccgcccgc
     1321 cagaccagca ccgagataat cggactgatg atggcgctca acatgtagaa ggcgaagtcc
     1381 aggcgatagg caagcgtcac cgccatgttg accgcggtca tccgccggat cagtcgcagg
     1441 gattggggga ccgtcatcgt gccaccaccg tctctcccgc gaagagtgac cggatgatct
     1501 cctcgacatc ggcgtcagcg acactgagat cgatcatcgg gccaagtcgc ggcagcagtt
     1561 cgagcagatc gctgagtccg tcgcgcggca cctcgagcgc gaccacgcgg ccatcgtcgt
     1621 cctcgaccgg aatcctggtc acgtcgccac ccaacgctga cgcatcgacc ggcgcggcga
     1681 aggtcgcgcg gatcaacttg cgcggttgca cgctctggac gagatcgacg agaccgccgt
     1741 cgtaggcgat ccgcccatgg ttgacgatca tcacccgcga acagagcgcc tcgatgtcgt
     1801 ccatgtcgtg actcgtgatc aggatcgtcg tcccgcgccg ctgattgact tccgcgagga
     1861 aggtgcggac acgggccttg gcgacgacat cgagcccgat cgtcggctcg tcgaggaaga
     1921 gcacctccgg cctgtggagg agcgccgcga ggagttccat cttcatgcgc tcaccgagcg
     1981 agagcttgcg cacctgcacg ttgagcagat cttcgacatc gagcatcgtc gcgagctcgt
     2041 ccacgctgtc gcggaagacg gcgtccggca ggccatacat ctccttgtgg aggagaaagc
     2101 tctcggccgc cgggacgtcc caccagagca tcgtcttctg gcccatcacg agggcgatcc
     2161 ggcgcagata ctccgtcttg cggtcgaacg gctggaatcc gaggacgtcg atccggccgt
     2221 cactcggatg gaggagaccg gtaagcatct tcagcgtcgt cgtcttgccg gcgccgttcg
     2281 gtccgagcag gccgacgatc tcgccgcgcg cgatccggaa gctgacccga tcgacggcga
     2341 ggcgcgtctc tgtctgacgc cggaagacgc tccgaaaggc accggcgaga cccggtgact
     2401 gacggtgaaa ggtgtaggtc ttggtgagac cgaccgcatc gatcgcgatc gtggaagctt
     2461 cggcggaacg aagcgtcgac gactccgcgg gcacgaaggt gcccggaatt gtctgcatca
     2521 tgtaattcac tcgcctgtcc gagatcgaat gtagcacgaa ccgatggcga ccacgctcgg
     2581 gtgggggtcg cgcttcgcat tcccggtgcg gcaaatgccg gagggatgcg atcggatcgt
     2641 aatccgggac ctagtgggtc aggttattac agatgctgcg cataaagacg cctttcattg
     2701 ccgacctggt ctcggaccat cctagcacaa cggctacggg cgtcgtagcg gtcagcttgt
     2761 ctggcccagc ggcgccggtc aatcaaggcg cgacaaccca caagatgcct cgccatcgca
     2821 cgtcttccgc accgtggcgg gtcaccccgt acgggtgatg catagtcagg gtttgatcat
     2881 gaacggacac ggcgtcggcg acgatggaag gtgcccactc cggggcgcgg ctgaccagtg
     2941 gaaaggcccc agccatggat cccgacgtcg aca
//
LOCUS       DASTMD010000160         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1085920, whole genome shotgun sequence.
ACCESSION   DASTMD010000160 DASTMD010000000
VERSION     DASTMD010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085920"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..>2913
                     /locus_tag="VFQ54_02450"
     CDS             69..>2913
                     /locus_tag="VFQ54_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163874.1"
                     /translation="MVEPNDQPPQATDLGTDAGCATSPNAHGGQDVFKWTVDDAGAAK
                     RWTISTSAIPGQAVKLEIYTVTIDANGVVTNPVKLTGVSGDSGQAASIDNVLVAKGTY
                     FVATAIGGGGAYELTVSPGDPVPAANDTGDHASPETALPLQDAFALSGDRASAVDDFA
                     WTLDDRAAATHWTLMLQGPIGTSPGLSIFGSDGTTLFSDNAGADGKLVIPDLGLAAGT
                     YVLSVGSSSDAAAPYILSAVAGDLRSPQAEDEPNDGIASGMPIALNGNSTTIHGRLAT
                     LDGNGSNTDYYTLTGDDSTAGRYLDVRVVWQSGPSRKLCLEDPAGNALQCAEGDTGAA
                     LHDLVLGKGAYTISISGDTDPAAEYLLKVDLKQKAVAGFETEPNDAQAQASAMVTAPG
                     GSFQGSGRFSAGDDDFFKFTVSGDAQLWEVEIMGTGVSHLGVVDASGTSGSSRNVAAG
                     TTDPTRIDDLYLTPGDHWLDIEGANGDYAVTVKPLGPPDPEGEREPNDNLDFSQAINL
                     QETRNGRITDAGDTDVYRFSLQNDTYVTIGLDSPADAVLGMSIREAAMTYTSLSAANP
                     GDALHYDAYLQAGDYSIWITASTPSEANYHLRIAINDPFDLPADLEPNDSIDEAQPLP
                     ASMIATGTVDSLASGGDIDWYILPASLEGQQVTVEYTPGTTITLWTAQTKDTSQSSVP
                     LISGTVDGSYLAALPAEVLTYLEVSGTGSYQVAIVPDGATPPAIASLVAATPGIATPP
                     ASPVAVNGVTASLKLDTTPVAAFWSNGQRLGGALTLTNDSAESRDLALTARTGNALWT
                     VDLDQTKVTVKPGATISIPVAVHIAPDAWSGQPIYVAIAASFTGTTDAASTFVLVVPN
                     ADAPPVNSEQYDPLPASLLGGLDVAWTGLGATPMPADGQDPTEVAQLFDQLTTTGTGL
                     SVDVSSLPQDYTVELAGTDPVPVAGFTLDPRGAG"
BASE COUNT          526 a          988 c          922 g          477 t
ORIGIN      
        1 gcgtgacggg ggcggacgcc gtagcggcgc agggagcgac gccgtcggga tcgtcgaact
       61 gtacgaccgt ggtcgaaccc aacgatcagc cgccgcaggc gaccgatctc gggacggacg
      121 cgggctgcgc cacgtcccca aacgcgcatg gtggccagga tgtcttcaag tggacggtcg
      181 atgacgccgg cgccgcgaag cgctggacga tctcgacctc cgcgattccg ggacaggccg
      241 taaagctcga gatctacaca gtcacgatcg atgcgaacgg cgtcgtcacg aatcccgtga
      301 agcttaccgg ggtgagtggc gactccggac aggcggcgtc gatcgacaac gttctggtcg
      361 ccaagggcac gtactttgtc gcgacggcga tcggcggcgg tggcgcgtac gaactcacgg
      421 tgtcgccggg cgatccggtg ccggcggcga acgacaccgg cgatcatgct tcgccggaaa
      481 cagcgctgcc gctgcaggac gccttcgcgc tttcgggcga tcgcgcaagc gctgtcgacg
      541 acttcgcgtg gacgctcgac gatcgagcgg cggcaaccca ctggacgctg atgctccagg
      601 ggccgatcgg gacatcgccc gggctttcca ttttcggctc tgatggcacg actctctttt
      661 ccgacaatgc cggagccgac gggaagcttg tcattcccga cctcgggctc gccgcgggga
      721 cctatgtgct ctccgttggg tcctcgtccg acgcagccgc tccgtatatc ctgagcgccg
      781 tcgccgggga cctccgttct ccccaggccg aggatgagcc gaacgacggg atcgcctccg
      841 gcatgccgat cgccctgaac ggcaattcga cgacgatcca cgggcgtctg gcgacgctcg
      901 atggcaatgg cagcaacacc gattactaca cgctcaccgg cgatgactcc accgccggac
      961 gctacctcga cgtgcgtgtc gtctggcaat ccggcccatc ccggaagctc tgcctggagg
     1021 atcccgcggg caatgcgctc cagtgcgccg agggcgatac cggcgccgcg cttcacgacc
     1081 ttgtgcttgg caagggcgcg tatacgatct cgatcagtgg cgataccgat cccgctgccg
     1141 aatacctgct gaaggtcgat ctcaagcaga aggcggtcgc gggcttcgag accgaaccga
     1201 acgatgccca agcacaggcg agcgcgatgg tgaccgcgcc cggcggctcc ttccagggga
     1261 gtggtcgttt ctccgccggc gacgatgact tcttcaagtt cacggtatcc ggggatgccc
     1321 agctctggga ggtggagatc atgggaaccg gggtgtcgca tctcggagtc gtcgatgcct
     1381 cggggacatc gggttcctcg cgcaacgtcg ccgccggcac gaccgacccc acgcgaatcg
     1441 acgatctcta tctgacgccg ggcgatcact ggctggatat cgaaggcgcg aatggagact
     1501 acgccgtcac cgtcaagccg cttgggccgc ccgatcccga aggggagcgc gagccgaatg
     1561 acaacctcga cttctcccag gcgatcaatc ttcaggagac gcgcaacgga cggatcaccg
     1621 acgcgggtga caccgatgtc tatcgcttct ctctgcagaa cgatacctac gtcacgatcg
     1681 gtctcgattc accggcagac gcggtactcg gcatgtccat tcgcgaggcc gcgatgacct
     1741 atacgtccct ctccgcggcc aatcccgggg acgcgctgca ctatgacgcc tatctccagg
     1801 cgggcgacta ctcgatctgg atcaccgcat cgacaccaag tgaggcgaac tatcacctca
     1861 ggattgcgat caacgatccg ttcgatcttc cggccgatct cgaaccgaac gactcgatcg
     1921 acgaagcgca accgctaccg gcgtcgatga ttgccacggg aacagtcgat tccctggcgt
     1981 ccgggggcga catagactgg tacatcctgc cagcctccct cgagggtcag caggtgacgg
     2041 tcgaatacac gccgggaacg accatcacgc tctggacggc ccagacgaag gacaccagcc
     2101 agagttcggt cccgctcatc tcggggaccg tcgacgggag ctatctcgca gcattgccgg
     2161 cagaggtcct gacctatctc gaggtatcgg gaacgggttc ctaccaggtc gcgatcgtcc
     2221 ccgacggtgc gacaccgcca gcgatcgcat cgctcgtagc ggcgacaccg gggattgcga
     2281 cgccgcccgc gtcgccagtc gcggtgaacg gtgtcaccgc atccctgaag ctggatacga
     2341 ccccggtcgc ggcattctgg tcgaacggcc agcggctcgg cggcgcgctc acgttgacca
     2401 acgacagcgc cgaatcccgc gatctcgcct tgacggcgag gacagggaac gcactctgga
     2461 ccgtcgacct cgaccagacg aaggtgaccg tgaagccggg cgcgacgatt tcgattccgg
     2521 tcgccgttca catcgctccc gacgcctggt ccggccagcc gatctacgtc gcgatcgcgg
     2581 catcgttcac ggggaccacc gacgccgcga gtacttttgt cctcgtcgtg ccgaatgccg
     2641 acgccccccc ggtcaacagc gaacagtacg atcccctccc ggcgtcgctg ctcggtggac
     2701 tcgacgtcgc ctggacggga ctcggcgcga caccgatgcc ggccgacggc caggacccca
     2761 ccgaagtcgc ccagctcttc gatcaactga cgaccacggg gacgggcctg agcgtcgacg
     2821 tctcgtcatt gcctcaggac tacacggtcg aactcgccgg aacggatccg gtgcctgtcg
     2881 ccggcttcac gctcgatccc cggggcgctg gtg
//
LOCUS       DASTMD010000161         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1085943, whole genome shotgun sequence.
ACCESSION   DASTMD010000161 DASTMD010000000
VERSION     DASTMD010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085943"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFQ54_02455"
     CDS             <1..586
                     /locus_tag="VFQ54_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEU0163875.1"
                     /translation="EAVTELRELMDFLGRLGVPRHAYKIDLALARGIDYYTGPVYEAI
                     VEEPKVGSVAGGGRYDGLIGMFLGRDVPATGISLGIERILEVVQEFDLFPIPKTTTDV
                     FVIALPDTLADAAHLARVLREQGINADQSVQITRGIGDQLKYADRLHIPLALIPGSAE
                     LANGKVSVKDLKSGEQKEIDISLLTEEIVRMLGR"
     gene            653..1357
                     /locus_tag="VFQ54_02460"
     CDS             653..1357
                     /locus_tag="VFQ54_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEU0163876.1"
                     /translation="MAEEPQNDHQTYMWVVAHPDDAEFSSAGTITKFSKEGKKTIIIQ
                     VTSGNRGTADRSFTPETLIATREAEEREAAKRMGASELVFLKQPDGGLVPDLELREKI
                     VRMIRTYRPDVIITHDPFRPYALHPDHRAVGMATHDAVYPTARDFLYYPEHLEAGLEP
                     HKTAEIWYFGAESPDVFIDITDTFDTKIEALKAHKSQVGEAVELADRLRERAAEVAAD
                     QPFELAESFKSIKMRR"
     gene            1444..>2431
                     /locus_tag="VFQ54_02465"
     CDS             1444..>2431
                     /locus_tag="VFQ54_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HEU0163877.1"
                     /translation="MSFWGEEELKTQAIATARTAFGESWTPSRWAIGPGRLELLGNHV
                     DYNGGLVLAGAIDRAVMIGISETGSPDKVEFITPDISSGRWEIDLDTIGDWHAAPGES
                     GTEAYARGVAASLLGRGIAICDELQLSIVGNVPLGFGMSSSAGLCVSLVLALAADTPS
                     DRDIVLIAQDGEHRAGAPVGAMDQSASVAGGIIIFDGADISFRRILPDLGDYVFAVAD
                     SGVDHSNATSSYPARVQESRAALEILRERLDPDLASLGAITPEQWSEVESSGDRWLDQ
                     TLLERTRHVVTEMARVREGIGAVKTADWSRFGQLMNASGRSSAIDYAISHPRV"
BASE COUNT          457 a          799 c          751 g          424 t
ORIGIN      
        1 cgaggccgtc accgaactcc gcgagctgat ggactttctc ggtcggctcg gggttcccag
       61 gcacgcctac aagatcgatc tcgccctggc gcgcggcatc gactactaca cgggcccagt
      121 ctatgaggcg atcgtcgagg agccgaaggt cggctccgtt gccggcggtg gccgttacga
      181 cggcctgatc gggatgttcc tcgggcgcga cgtcccggcg accgggattt cgctggggat
      241 cgagcggatt ctcgaagtcg tccaggagtt cgatctcttc ccgatcccga aaaccacgac
      301 cgacgtcttc gtcatcgccc tgcccgatac gcttgcggac gccgcgcatc tcgcaagagt
      361 tctccgcgag cagggcatca acgccgacca gtcggtccag atcacgcgtg gcatcggcga
      421 tcagttgaag tacgcggatc gcctgcacat tccgctggcg ctcattccgg gctccgccga
      481 gctcgcgaat ggcaaggtga gcgtgaagga cctgaagtcg ggagaacaga aagagattga
      541 catctctctc ctgacggagg aaattgtgcg gatgctggga cgttaggggc aattcgctgc
      601 atcacccggc aatccaccac gaccgcaaga tcatcgcatg ggggagactc aattggctga
      661 agaaccacag aacgaccatc agacctatat gtgggtggtc gcccacccgg acgatgccga
      721 attctcctcg gcgggaacga tcacgaagtt ctcgaaggaa ggcaagaaga cgatcatcat
      781 ccaggtgacg tcgggcaatc gcggaaccgc cgatcgctcg ttcacgccgg agaccctgat
      841 cgcgacccgc gaggcagaag aacgcgaagc cgcgaagcgg atgggcgcca gcgaactcgt
      901 cttcctcaag cagccggacg gcggcctcgt ccccgacctc gagcttcgtg agaagatcgt
      961 ccgcatgatc cgcacctatc gcccggacgt gatcatcacc cacgatccgt tccgcccgta
     1021 cgcgctgcat ccggaccacc gtgcggtcgg catggccacg cacgacgccg tctatccgac
     1081 ggcccgtgat ttcctctact acccggaaca cctcgaagcc ggtctcgagc cacacaagac
     1141 cgccgagatc tggtacttcg gcgcggagtc gccggatgtc ttcatcgaca tcaccgacac
     1201 cttcgacacc aagatcgagg cgctcaaggc ccacaagtcg caggtcggcg aggccgttga
     1261 actggccgat cgcctgcgcg agcgtgccgc cgaagtcgcc gccgaccagc cgttcgagtt
     1321 ggccgaatcg ttcaagtcga tcaagatgcg ccgctgatcc cgagacaccg cactacattc
     1381 cgttcaacca tgtggcggga cgttcgcgtc ccgccttcct ccgatgtggg agccatccag
     1441 acaatgtcgt tttggggcga ggaagaactg aagacgcagg cgattgcgac tgcaagaacg
     1501 gcgttcggcg aaagctggac gccttcgcgt tgggcgatcg gaccgggtcg actcgaactg
     1561 ctgggcaatc acgtcgacta caacggcggg ctcgttctcg ccggcgcgat cgatcgcgcc
     1621 gtcatgatcg ggatctcgga gaccggctcg ccggacaagg tcgaattcat tactcccgac
     1681 atcagctccg gcagatggga gatcgacctc gacacgatcg gggactggca tgccgcgccc
     1741 ggcgaatcgg gcacggaagc ctatgccagg ggcgttgccg catccctgct cggtcgcggg
     1801 atcgcgattt gcgacgagct ccagctctcg atcgtgggca atgtcccgct cggcttcggc
     1861 atgagttctt cggcgggcct ctgcgtctcg ctcgtccttg ccctagccgc cgacacccca
     1921 tcggaccgcg acatcgtcct gatcgcgcag gacggcgaac accgggcggg ggcacccgtc
     1981 ggcgcgatgg atcaatcggc gtccgttgcg ggcggcatca tcatcttcga cggcgccgac
     2041 atctccttcc ggcggattct tcctgatctc ggcgattatg tcttcgccgt tgcggactcc
     2101 ggtgtcgacc acagcaacgc gacatcctca taccccgcac gtgtccagga gtcccgcgcc
     2161 gcgctcgaga tccttcgcga gcgacttgat cccgatctcg cctcgctcgg cgcgatcacg
     2221 ccggagcagt ggtcggaagt cgaatcatcc ggcgacaggt ggctcgacca gacccttctt
     2281 gagcgaacca ggcacgtcgt gacggagatg gcgcgcgttc gcgaagggat cggggccgtc
     2341 aagacagcgg actggagtcg cttcggccag ctgatgaacg cctcgggacg ctcgtcggcg
     2401 atcgattacg cgatcagtca tccccgggtc g
//
LOCUS       DASTMD010000162         3094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2425275, whole genome shotgun sequence.
ACCESSION   DASTMD010000162 DASTMD010000000
VERSION     DASTMD010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2425275"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VFQ54_02470"
     CDS             <1..921
                     /locus_tag="VFQ54_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0163878.1"
                     /translation="SVRFDVVNPSNIGDTVHGTTILVPLMSRITGELMDRFEGLRLIQ
                     QWGAVLEGVDINAATARGIAVGNIPTTTSGNANSVAEWCVMAALAMSRQLLDHNLLMR
                     EGTRWGSPIGQALYGRRAGIIGLGGIGNVLARRLGAFEMDVQAVTRSPDAASAAASGV
                     RGVRGMESLGELLAESDYLFLTLPLTPETRHLIGENALAAMKPGAYLINAGRGGLVDP
                     GALIAALDRGQLGGAALDVFEQEPLDPASPLLTHPRILVSPHVAGVTDIFYRSAASVF
                     VDALDAIAGGRPLTHCVNWSAVAKHFYAEP"
     gene            complement(977..1570)
                     /locus_tag="VFQ54_02475"
     CDS             complement(977..1570)
                     /locus_tag="VFQ54_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU0163879.1"
                     /translation="MSAAIEVMNEHGLDKVSLRAIATRLDIRQPGLYHHFASKADLLD
                     AVADEILDRWHTDRLPEGDERWDEFVARNARSLRRAMLSVRDGARLIASVGSRSPRLD
                     NAIAQLALLENAGFSGPEAVLALIAVSRYAIGSAVEQQTARDRGDIVINTDRTDAAAS
                     HLIEIARQVVALGQDHEFEIGLAALLRGLAPTAPGVR"
     gene            1918..2121
                     /locus_tag="VFQ54_02480"
     CDS             1918..2121
                     /locus_tag="VFQ54_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163880.1"
                     /translation="MKQEDAWTATVLKKSRALFDGSNLYRQVTIRRDDDETETIRVNR
                     ALWKEINVGDRLVKDPGQEPRRE"
     gene            complement(2170..>3094)
                     /locus_tag="VFQ54_02485"
     CDS             complement(2170..>3094)
                     /locus_tag="VFQ54_02485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEU0163881.1"
                     /translation="LMSRAVIYDKFGGPEVLELREVPEPHPGQGEVRVHVSACGLNPM
                     DWGIAGSPEAAARFGIILPSGFGYDFAGVIDEVGDGAEGYAIGDRVYGGALARAAADF
                     VVVATTDNGLFHTPEGITDEVAATLPVAGHTAAAALAAVGLQSGDTILIGGAAGGVGV
                     FAVQLARLAGATVIGTASPSTFEFVRELGAVPVAYGAGLADRVRALAPNGVTAATDLF
                     GTETVETALALGVPAARISTIAAGPNPPGGVRATGGIEGGPDSMKRITDAILAGTLTV
                     PIAATFPIEQIRDAVTLQAGRHVHGKIVITL"
BASE COUNT          558 a          975 c         1044 g          517 t
ORIGIN      
        1 tcggtgcgat tcgacgtcgt gaatccctcg aacatcggcg atacggtcca cgggacgacg
       61 attctggtgc cgttgatgtc ccggatcacc ggtgaactga tggatcgttt cgaggggctc
      121 cggctgatcc agcagtgggg agcagtactc gaaggggtcg acataaacgc ggcgacggcg
      181 cgggggatcg cggtcgggaa catcccgacg acgacgtccg gaaacgcgaa ctcggtcgcg
      241 gagtggtgcg tgatggcggc gctcgcgatg agtcgccaac tcctcgacca caacctgctg
      301 atgcgcgaag ggacgcgttg gggaagtcca atcgggcagg cgctctatgg caggcgagcc
      361 ggaatcatcg gactcggcgg catcggaaac gtcctggcgc ggcgtctggg cgcgttcgaa
      421 atggatgtcc aggcggtcac acggagcccg gacgcggcgt ccgcggcggc gtctggagtc
      481 cgtggcgtac ggggcatgga gtcgcttggc gagctactcg ccgagtcgga ctatctcttc
      541 ctgaccctgc cgctgactcc cgagacgcgt cacctgatcg gagagaacgc gctggcggcg
      601 atgaagccgg gcgcctacct catcaatgcc gggcgtggcg gcctcgtcga tccgggggcc
      661 ctgattgccg cgctcgatcg tggccaactt gggggtgcgg cgctcgatgt cttcgagcag
      721 gagccgctgg atcccgcgtc gccactcctg acccatcccc gaatcctcgt ctcgccccat
      781 gtcgcgggtg tgaccgacat cttctatcga tcggcggcgt cggtcttcgt cgatgcactg
      841 gatgcgatcg cgggcggaag gccgctgacg cattgcgtca actggtctgc cgtcgcgaaa
      901 catttctacg ccgaaccatg aacgaaccag cgggagtccg ccgcggtggg caagagcgag
      961 ctccggaaga caatcctcaa cgaactccgg gagccgtggg agcgagtcct cggaggagcg
     1021 cggccaggcc gatctcgaac tcgtggtctt ggccgagggc gacgacttgt cgtgcgatct
     1081 cgatgaggtg actcgccgcc gcatcggttc gatccgtgtt gatgacgatg tcaccacgat
     1141 cgcgcgcggt ctgctgttcg accgccgaac cgatggcata gcgggagacg gcgatcagag
     1201 cgaggactgc ctccggtcca ctgaagccgg cattctcgag gagggccagc tgggcgatcg
     1261 cattgtcaag acgtgggctc ctggaaccca ctgatgcgat cagccgggca ccgtcgcgga
     1321 cgctcagcat cgcgcgcctc agactccgcg cattcctggc gacaaactca tcccatcgct
     1381 catcgccctc aggaaggcgg tcggtatgcc agcggtcaag gatttcgtcc gcaacggcat
     1441 cgagcaagtc ggctttgctt gcgaaatggt ggtagaggcc cggttggcgg atgtcgaggc
     1501 gcgtggcgat cgcccggagc gagaccttgt cgagtccgtg ctcgttcatc acctcgatcg
     1561 cggccgacac gatcgatggc ctgtccgtgc gtcgtggatg tggcatggat cggacactac
     1621 cctgtgagtt cgttcagcga taacgcatgc gatgagacag aagatgccgg gctcgctgcc
     1681 aagtctagcg gaactgcgat gcgtccaacc tgggggacgg gtacggcgat cgtgtcgacc
     1741 gggcagggac agaccccggt gacgaggata tctgccgccg cgaccgttca cgtcgcagtc
     1801 tccctcaccc ggttctcact gcactccagg ggtctcggac gcttgcacga acgaccatcc
     1861 tggataaact atcaatgata accttcgatc cggtgcgaac gcacgaaagg gaatgacatg
     1921 aagcaggaag acgcgtggac cgcaacggtg ctgaaaaagt cgcgcgcgct gttcgatggc
     1981 tcgaacctgt atcgacaggt caccatccgc agagacgacg acgagaccga gacgatccgg
     2041 gtcaaccgag cgctatggaa ggagatcaac gtcggtgacc gcctcgtgaa ggaccccggt
     2101 caggagcctc gtcgcgagtg acgaatgcgc gtatctgccg gcgacctgac cggacggaat
     2161 caggcgttgt cacagcgtga tcacgatctt gccgtgaacg tggcgcccgg cctggagcgt
     2221 caccgcgtcg cggatctgtt cgatcgggaa ggtcgcggcg atgggcaccg tcagcgtgcc
     2281 ggcaaggatc gcatcggtga ttcgcttcat cgagtccggg cctccctcga tgccgccggt
     2341 ggcgcgcacg ccgccgggag gatttggacc tgcggcgatc gtggagatcc gcgcggccgg
     2401 cacgccgagc gcgagcgcgg tctcgacggt ctcggtgccg aacagatccg tcgccgcggt
     2461 cacaccattg ggagccaacg cccgcacccg gtccgccagt cccgcgccat aagcgacggg
     2521 caccgcacca agttctcgca cgaactcgaa ggtgctcggg gaagcggttc cgatcacggt
     2581 cgcgccggcg agcctggcga gttgcacggc gaacacgccg actccgcccg cggcgccacc
     2641 gataaggatg gtgtcgccgg actgcagacc gacggccgcc agagcggcag cggcggtgtg
     2701 gccagccacc ggcaacgtcg ccgctacctc gtcggtgatg ccttccgggg tgtggaagag
     2761 gccattgtcg gttgtcgcca ctaccacgaa gtcggcggca gccctcgcga gcgcaccacc
     2821 gtagacgcga tcgccgattg catatccttc cgcgccatcg ccgacctcgt cgatgacccc
     2881 ggcgaagtcg tagccgaatc cggaaggcag gatgatgccg aaccgggcag ccgcttcggg
     2941 gctgccggcg atgccccagt ccatcggatt caggccgcat gctgaaacgt gaacgcggac
     3001 ctctccctgg ccagggtgag gttcgggaac ctcgcgcaac tccaggacct cggggcctcc
     3061 aaacttgtca tagatcacgg ctcggctcat gagt
//
LOCUS       DASTMD010000163         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_104460, whole genome shotgun sequence.
ACCESSION   DASTMD010000163 DASTMD010000000
VERSION     DASTMD010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104460"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /gene="coaBC"
                     /locus_tag="VFQ54_02490"
     CDS             complement(<1..1217)
                     /gene="coaBC"
                     /locus_tag="VFQ54_02490"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEU0163882.1"
                     /translation="MSAPDATWRGLRGAKVVLGVTGGIAAYKAADLASKLVQAGAIVN
                     VVMTEAATQFVQPMTFTALTHRAVYTDIFDGWSEESRGHVTLAAEADLVVIAPATANT
                     IARLAHGLVDDMLAAVCLATSAPIVVAPAMEHHMWHHPATVANIATLRERGVTIVDPE
                     SGYLASGASGDGRLASTGRILAVLRGVQGRGGPLAGSRVVVTAGGTQEALDPVRYLGN
                     GSTGTMGLALVEAAIDLGADVTAIVGPTVTPGPTTAKIVRIVSAREMATAVNDATGDA
                     DVLVMAAAVADFRPREFSDQKIKKTPGQDDLQIDLVRNPDIIAGVKQPGLLKIGFAAE
                     TERLEEYAMGKLRGKGLAMIVANDAATTIGSPDSEATLLFPDREPVRLPKMSKEDVAR
                     AVLSEVARLLHVAT"
     gene            complement(1204..2316)
                     /locus_tag="VFQ54_02495"
     CDS             complement(1204..2316)
                     /locus_tag="VFQ54_02495"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEU0163883.1"
                     /translation="MSTASRTDASISTTQDASPRFIDLRSDTVTRPTPEMRRAMAESL
                     VGDDQYGEDPTVNELEAQAAELMGKEAAVFVPSGTMGNLSSIMARCQRGVEAIVGSES
                     HILWYESGGPSTLAGVPLFTIPNDRWGRLDLDQVRASIREDRAGFPQTGVICIENTHN
                     RCGGVVLSVDYLRDLRAVAQERGVPIHMDGARIFNAAAALGVPVSDLAAQVDSVQFCL
                     SKGLAAPIGTLVTGDAAFIQSVRKQRKVLGGAMRQAGVVAAAGLVAFETMIDRLPEDH
                     RRARTLAEGLAQIPGVAVDLETVQTNIIVFKVPAGRDRLAAIDAFKERGLMVSDYGLK
                     GLRLVTHYEIDDAAVASALTIIESVLTAPEGARVSA"
     gene            complement(2467..>3553)
                     /locus_tag="VFQ54_02500"
     CDS             complement(2467..>3553)
                     /locus_tag="VFQ54_02500"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0163884.1"
                     /translation="GAIHQAGLVQRDMKPQNILIGTDGQARVADFGIAQVEVDTGLTA
                     AGSAIGTAAYMAREQAQGGRTTPATDLYAVGVVLYEMLTGVLPFTAPTSMAMMLAHIQ
                     QQAAPPSLRSNGRPVPPDLDGIVMQALAKDPRDRFRSAQAMMQALRGDSTTIAATQAT
                     RMTAPQQAATQRFDSGAIRPGATTQTPRRAAAGGTPPPPPTRLDWLEDERRGGGPGGA
                     LAGLLLVVLIGLVAFGGYIWWNDRQDNGVASGTPTPTVTTFVTVTAPPTDVPTEQPPP
                     TDDAQLIEPQNTIASEPTATDAPTEPPPTAAPTQVPPTQEPSSTAAPTLAPTANEIIP
                     EPSATTAASDPGDSPPIQPIGTAAGQG"
BASE COUNT          554 a         1243 c         1160 g          596 t
ORIGIN      
        1 gttgcgacat gcagcaaccg ggcgacttcg ctcaggaccg cgcgtgcgac gtcctccttg
       61 gacatcttcg gcaaccgaac gggctcgcga tcggggaaga gcagcgtcgc ctcgctgtcc
      121 gggctcccga tcgtcgtggc cgcgtcgttg gccacgatca tcgccaatcc cttgccgcga
      181 agtttcccca tcgcatattc ctccagacgt tccgtctccg ccgcgaagcc gatcttcaac
      241 aggccgggtt gcttcacccc ggcgatgata tccggattgc gcacgaggtc gatctggagg
      301 tcgtcctgtc ccggcgtctt cttgatcttc tgatcggaga actcgcgcgg acggaaatcc
      361 gcgaccgccg ccgccatgac caggacatcg gcgtccccgg tcgcatcgtt taccgccgtc
      421 gccatctctc gcgccgaaac gatgcgcacg atcttcgccg tcgtcggacc cggcgttacg
      481 gtcggaccga cgatcgccgt cacgtccgcg ccgagatcga tcgccgcttc gacgagcgcc
      541 aggcccatcg tgccggtcga tccgttcccg agatagcgca cggggtcgag cgcctcctgc
      601 gtgccgccgg ccgtgaccac aacgcggctc ccggcgagcg gcccgccgcg gccctggaca
      661 ccccggagta ccgcgagaat ccgcccggtc gaggcaagtc tcccgtcacc ggacgcaccg
      721 gacgcgagat agccggattc gggatcgacg atcgtcactc cccgctcgcg gagcgtcgcg
      781 atattcgcaa cagtggcggg gtgatgccac atgtgatgtt ccatcgccgg tgcgacgacg
      841 atcggcgccg aagtcgcgag gcagaccgcc gccagcatgt cgtcgacaag gccgtgcgcc
      901 agacgcgcaa tggtgttcgc cgtggccggc gcgatgacga cgagatcggc ttccgccgcc
      961 agtgtgacgt gaccgcgcga ttcctcactc cacccgtcga agatatccgt gtagacggcc
     1021 cgatgcgtca acgccgtgaa ggtcatcggc tgcacgaact gcgtcgccgc ctccgtcatc
     1081 acgacattga cgatggcgcc ggcctgaacg agcttgctgg cgagatctgc cgccttgtag
     1141 gccgcgatcc cgccggtaac gccaaggacg accttcgcgc cgcgaagccc gcgccaggtc
     1201 gcatcaggcg ctgacacgcg cgccctccgg agcggtcagc acggattcga tgatcgtcaa
     1261 cgcggaggca accgccgcat cgtcgatctc gtaatgggtc acgagtcgaa gccccttcag
     1321 tccgtaatcc gaaaccatca gaccacgctc cttgaaggcg tcgatcgcgg cgaggcggtc
     1381 acggccggct ggcaccttga agacgatgat gttcgtctgg accgtctcga gatccacggc
     1441 cacgccgggg atctgcgcga gcccctcggc caacgtccgg gcgcgccggt gatcctccgg
     1501 aaggcgatcg atcatcgtct cgaaggcgac gagtcccgcc gccgcgacga ccccggcctg
     1561 tcgcatcgca ccgccgagca ccttgcgctg cttgcgaaca ctctggatga acgccgcgtc
     1621 gccggtcacc agggtcccga tcggtgccgc gagccccttg ctgaggcaga actgcaccga
     1681 atcgacctgg gccgcgagat cggagaccgg cacgccgagt gccgccgccg cattgaagat
     1741 gcgcgccccg tccatgtgaa tcgggacacc gcgctcctgg gcgactgcac gcagatcgcg
     1801 cagatagtcg accgagagca cgacgccgcc acagcggttg tgcgtgttct cgatgcagat
     1861 cacaccggtc tgcgggaatc ccgcgcgatc ctcgcgaatc gacgcccgca cctggtcgag
     1921 atcgagacgc ccccagcggt cgttcgggat cgtgaagagg ggcactccgg ccagcgtgga
     1981 tggtccaccg ctttcatacc agaggatgtg cgattcgctg ccgacgatcg cttcgacgcc
     2041 tcgctggcag cgcgccatga tcgacgagag atttcccatc gtgccgctgg gaacgaagac
     2101 cgcggcttcc ttccccatca actcggcggc ctgcgcctcg agctcgttga cggttggatc
     2161 ctcgccgtac tggtcgtcgc cgacgagcga ctccgccatc gcgcggcgca tctccggtgt
     2221 cggccgggtg acggtgtcgg accgcaggtc gatgaaacgc ggggacgcgt cctgggtggt
     2281 cgatatcgag gcatcggttc gcgaggctgt gctcatggat ctcctccgga actgggttct
     2341 acgggttgct atggtacgcg aatcgatgtc cgctcttgcc ccgtatcatc gcatgccagc
     2401 cgctcgatcg cgtcccaccc tcgggatcgc tctcccgatt ccgagcgggc gcggatccga
     2461 cccggctcat ccctgaccgg ctgccgtgcc gatcggctgg atcggcggcg aatcgccagg
     2521 atccgaggcc gccgtcgtcg ccgatggctc gggaatgatc tcgtttgccg tcggcgccag
     2581 ggtcggcgcc gcggtcgaag aaggttcctg tgtcggcgga acctgtgtcg gcgcagccgt
     2641 cggtggcggc tccgtgggag cgtcggtcgc cgttggctcc gacgcgatcg tattctgcgg
     2701 ttcgatcaac tgcgcatcat cggtcggcgg cggctgctcg gtcgggacat ccgttggggg
     2761 cgccgtgaca gtcacgaacg tcgtcaccgt tggggttggc gtacccgagg cgacgccatt
     2821 gtcctgacga tcgttccacc agatgtatcc gccgaatgcc acgagcccga tcagcacgac
     2881 gagcaacaga ccggcgagag ccccgccggg accgcctccc cggcgctcat cctccaacca
     2941 gtcgagccgc gtcggaggtg gaggcggcgt ccccccggcg gccgcgcgcc gaggcgtctg
     3001 cgtcgtggct cctggccgga tggcaccgga atcgaaccgc tgtgtcgccg cctgctgcgg
     3061 ggcggtcatc cgggtcgcct gcgtcgcggc gatcgtcgtc gagtcgccac gaagcgcctg
     3121 catcatcgcc tgcgccgatc ggaagcgatc gcggggatcc ttcgccagcg cctgcatcac
     3181 gatcccgtcg agatcgggag gaaccgggcg cccgttgctg cgaagcgatg gcggcgcggc
     3241 ctgctgctga atgtgcgcga gcatcatcgc catcgaggtc ggcgccgtga atggcaggac
     3301 tcccgtgagc atctcgtaga ggacgacccc gacggcgtaa agatccgtcg ccggcgtcgt
     3361 acgtccaccc tgcgcctgct cgcgcgccat gtaggcggcc gtgccgatcg cggaacccgc
     3421 ggccgtcagc ccggtgtcga cctcgacctg ggcgatcccg aagtcggcga cacgtgcctg
     3481 gccatcggta ccgatcagga tgttctgcgg cttcatatcg cgctgcacga gtccggcctg
     3541 gtgaatcgcc ccg
//
LOCUS       DASTMD010000164         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1800973, whole genome shotgun sequence.
ACCESSION   DASTMD010000164 DASTMD010000000
VERSION     DASTMD010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800973"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2326)
                     /locus_tag="VFQ54_02505"
     CDS             complement(<1..2326)
                     /locus_tag="VFQ54_02505"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0163885.1"
                     /translation="MASQSASTATSPLVTHTLPNGLTIFLRPNHAAPVASFWAWYRVG
                     SRNEIPGYTGVSHWVEHMQFKGTPTQAKGSIFGRVSREGGSLNAMTSMDWTAYYETLP
                     ADRIDLSLAIESDRMTNSLFEADEVESERTVILSERLGAENRQTYFLAEEVMGAAFQA
                     HPYRHMVIGYEGDLRHISRDDLYGHYRRFYAPNNAFLTVAGDFDADEMVVRLEKAFGE
                     IPANPDPLPYVAQEPPQRGERRVIVRKASPAAYMMMAYRMPEARHPDIAPLLVADAVL
                     SGAKPMGMGGGSAMGRSSRLYRALVATGLARSASSGTGLHIDPHLWTFSATALPGVEP
                     ERIEAAVEAEIHRLQTELATEDEFTKARKQIRAQYVYSNETVTSQAYWLGQMEIVDHA
                     SRVDTLASELEAVTPEDVRRVAQTWLTPASRIVGWQLPEETVISAAPGGDDAVGDVAN
                     FTAAEPHRAWGYAGEEISTHGFVRTELPNGIVVLAQPRPGSPAISAAISVEAGQVSTG
                     DTVAGRSSMVARMLNRGTEKHRFEELNEATDGLGAMLGVDSEREAIDVSFHGLTEDFD
                     RLLELASEVVRTPIFPDDELERVRQQSLTGPREQEDDTGAMAGRALRELLYPEGHPYR
                     LPLGGDIESVSAMTRSELAAYHERTFGPSVTTISVVGGIENLEQATDAIQRVFGDWTA
                     SVPAPLDPPAVHPIAATERSFRLVPGKSQSNLTIAYPTIDRHHPDYFALTTANVILGQ
                     LGLMGRLGASVRDLQGLAYHVSSSVGGGMVNSLWT"
     gene            2489..2857
                     /locus_tag="VFQ54_02510"
     CDS             2489..2857
                     /locus_tag="VFQ54_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163886.1"
                     /translation="MQNRPSPTYDERLVDRVIAYLQTRDPRIFPFTAAFDEERERAFI
                     RDLRDGLADLSDSGSKRGTSSTGFLMNDWRLIQIVEEWAQARGGWPQGTDPAEPITRL
                     AAFDPPKSEEMDQVPFAAGE"
BASE COUNT          531 a          978 c          987 g          517 t
ORIGIN      
        1 ccgtccagag gctgttgacc atcccgccgc caacggaact cgagacatga taggcgagcc
       61 cctggagatc gcgcacgctg gcgccgagcc ggcccatcag acccaactgg ccgaggatca
      121 catttgccgt cgtcagcgca aagtaatccg ggtgatggcg atcgatcgtc ggataggcga
      181 tcgtcaggtt cgactggctc ttgccgggga cgaggcgaaa gctccgctcg gtggcggcga
      241 tcggatgaac cgccggcggg tcgagcggcg cgggaacgga cgcggtccag tcgccgaaca
      301 cgcgctgaat ggcgtcggtc gcctgttcaa ggttctcaat cccgccaacg accgagatcg
      361 tcgttaccga gggcccgaat gtccgctcgt gataggccgc gagttcgctg cgcgtcatcg
      421 cggagacaga ctcgatatcg cctccgagcg gcaggcggta cggatggccc tccggataga
      481 gcaattcacg cagggcacgc cccgccatcg cgcccgtatc gtcctcctgc tcgcgcggac
      541 cggtcaacga ctgctggcgg acgcgctcca gttcgtcgtc cgggaagatc ggtgtccgca
      601 cgacctcgga ggcgagttcg aggagtcgat cgaagtcctc ggtcagcccg tggaaggaga
      661 cgtcgatcgc ttcccgctcg gaatcgacgc cgagcatggc cccgagaccg tccgtcgcct
      721 cattcagttc ctcgaagcgg tgcttctcgg tgccgcgatt gagcatccgc gcgaccatcg
      781 aggagcggcc ggcgacggta tcccccgtcg agacctgacc ggcctcgaca ctgatcgccg
      841 ccgagatcgc cggggaaccg gggcgcggct gcgccagcac cacgatcccg ttcggaagtt
      901 cggtccgcac gaaaccatgc gtggagatct cctcgccagc gtagccccag gcgcgatgcg
      961 gttccgccgc ggtgaagttc gcgacatccc cgactgcgtc atcgccgcca ggcgccgccg
     1021 agatgaccgt ctcttctggc agctgccagc cgacgatacg actcgccgga gtcaaccagg
     1081 tctgggcgac gcggcgcacg tcttccggcg tcaccgcttc gagttcgctg gcgagcgtgt
     1141 caacccggga cgcgtgatcg acgatctcca tctgtccgag ccaatacgcc tgcgacgtca
     1201 ccgtctcgtt cgagtagacg tactgggcgc gaatctgctt ccgtgccttc gtgaactcgt
     1261 cctcggtcgc cagctccgtc tgcagtcggt ggatctcggc ttcgaccgcc gcctcgatcc
     1321 gctccggttc gacaccgggc agtgccgtgg ccgagaaggt ccagagatgc ggatcgatgt
     1381 gcagcccggt gcccgatgac gccgagcggg cgagtccggt cgcgacgagc gcgcggtaga
     1441 ggcgcgacga tcgtcccatc gcgctccctc cgcccatgcc catcggcttc gcaccggaaa
     1501 gaacggcgtc ggcgaccagc agcggggcga tgtccggatg gcgggcctcc ggcatccggt
     1561 acgccatcat catgtaggcc gccggactcg ccttgcgcac gatcacgcgg cgttcgccac
     1621 gctggggcgg ttcctgcgcg acgtagggga ggggatccgg attcgccggg atctcgccga
     1681 atgccttctc cagcctcacg accatctcgt cggcatcgaa gtcgcctgcc accgtcagga
     1741 aagcgttgtt cggcgcgtag aagcggcggt agtgaccgta gagatcgtcc cggctgatgt
     1801 ggcggaggtc gccctcgtag ccgatcacca tgtggcggta gggatgagcc tggaaggccg
     1861 cacccatcac ttcttcggca aggaagtagg tctggcgatt ctcggcgccg aggcgttcgg
     1921 agagaatgac cgtccgctcg ctctcgacct catcggcttc gaagagcgag ttcgtcatcc
     1981 ggtccgattc gatcgccagc gagagatcga tgcggtcggc agggagtgtc tcgtagtagg
     2041 cagtccagtc catcgaggtc atcgcgttga gcgatccgcc ttcacggctg acgcgtccga
     2101 agatcgaccc cttcgcctgg gtcggcgtgc ccttgaactg catgtgctcg acccagtgcg
     2161 agacaccggt gtagccggga atctcgttgc gactgccgac ccgataccag gcccagaaag
     2221 acgcgacggg agcggcatgg ttggggcgta ggaagatcgt gaggccgttc ggaagggtgt
     2281 gcgtcacgag cggactggtc gcggttgatg ctgattgaga agccatggaa tcgggagtcc
     2341 ttctgcaccg gcagcgacgc cggaagacga aaatgcaatg cgaaaaggat acgcggtcgc
     2401 attgcgtccc gctgagtcgt tcgggcagta caattcgttc cagtcagcga aggtttgcca
     2461 aacaatctca acgaggagcg tggaaaccgt gcagaaccgt ccctccccga cctacgacga
     2521 gcgactcgtc gaccgcgtca tcgcctacct gcagacgcgc gatccgcgaa tcttcccgtt
     2581 caccgcggca ttcgatgagg aacgtgagcg agcgttcatc cgcgacttgc gggacgggct
     2641 cgccgatctc tctgacagcg gcagcaagcg cgggacgtca tcgaccggat tcctgatgaa
     2701 cgactggcgg ctgatccaga tcgtcgagga atgggcccag gcgcggggcg gctggccaca
     2761 aggcacggat ccggcggagc cgatcacgcg gctcgccgcc ttcgatccgc cgaaatccga
     2821 ggagatggac caggttccct tcgccgcggg cgagtagtcc ggcccctgcc tcgccattca
     2881 cagtcttccg tactcccgta gatgcggcga gagatgaact ccaacggtgc cgcatgcaag
     2941 tttgtcattt cgagcttgtc gagaaatccc cgtgcgaagc acatccggcg tagccggact
     3001 ctgacgccag tgt
//
LOCUS       DASTMD010000165         1675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_283369, whole genome shotgun sequence.
ACCESSION   DASTMD010000165 DASTMD010000000
VERSION     DASTMD010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283369"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            560..1036
                     /locus_tag="VFQ54_02515"
     CDS             560..1036
                     /locus_tag="VFQ54_02515"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0163887.1"
                     /translation="MFRHLDLHISLLTHADVDACFAIGTSLPSWFGDEVGLAEMRRSL
                     AMDEGYVARIHDRVVGFVTCARPFVETWEIAWLAVVEDLHRRGVGRKLVDAVIAKAHS
                     ECVSMLTVKTLADTHPSPEYAETRAFYAGIGFVRIVALPEYWSRDNPCLILARSLD"
     gene            complement(1161..>1675)
                     /locus_tag="VFQ54_02520"
     CDS             complement(1161..>1675)
                     /locus_tag="VFQ54_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163888.1"
                     /translation="SAVDIAIGVGDELGIDSGPAPTAAPKPTKTPKATEEPQPTEESG
                     TLTKSEYLADIRKEVDARDTEMTKLLAILDRVSSQTETDQDYTDLANIITTWMALPDY
                     DAPAGVSEIDDTYKAMNKNLSEGALNFTTALTGGSEADTDLLSQAIGQFQDAQSLIAQ
                     LDDLLTAEGV"
BASE COUNT          261 a          525 c          569 g          320 t
ORIGIN      
        1 ccgttcgcgg ggaggcgagg cgtcgccagc aggcgggctg cgtccccgga actaccaaac
       61 ggtcgtccga gaccatgggc ggggccgggg gacgctgcca ccccccaaac atacaagggc
      121 ggggctttat gccccgccgc agacacaaac gccccgttcg caatgcctcg cgatccacga
      181 ccgtccggaa atggatcggc gtcgtcgcat tgatccgggg cgaccacgtg ggtcgccggg
      241 acgggctgcc aacggagcct gccccgagcg cagccgaggc gggacacgac gccgaccgcc
      301 gcgcagacga ccttgctgac gaccgggttg gccgggtggg gctttatgcc ccgccgcacc
      361 ctcagcctca atgcacggcg ccttgcgacc cacgaacggc gacccggaac ggcgacaagc
      421 gccgtgggga cgggggtggt gggagtgggg acggggtttg gaagatggcg ggttgcggcg
      481 ggacaggcgc ggatcacggt atcctgtcac gaacgtcgct cagtcccgtc ataccgcccc
      541 gacctcgagg atcgcgacca tgtttcgcca tctcgatctc cacatctcgt tattgaccca
      601 tgccgatgtc gacgcctgct tcgcgatcgg cacgtcgctc ccgtcgtggt tcggggatga
      661 ggtggggctt gccgagatgc gacgatcgct ggcgatggat gagggatacg tcgcgcgtat
      721 ccacgatcgt gtcgtcggat tcgtcacctg cgcgcgacca ttcgtcgaga catgggagat
      781 tgcctggctg gccgtggtcg aggacctgca ccggcgcggc gtcgggcgca agctcgtcga
      841 tgccgtgatc gcgaaggccc attccgaatg tgtctcgatg ctgaccgtga agacgctcgc
      901 cgacacgcat cccagtcccg agtatgccga gacgcgggcc ttctatgcgg ggatcggctt
      961 cgtccgcatc gtcgccctgc ctgaatactg gtctcgcgac aatccctgcc tcatcctggc
     1021 gcgatcgttg gactgacggt cctcaacgcc gggttgatag cgcgcggggc ggtgttgcca
     1081 tcgcgccggg aaaggaaagg cggcggctcc gttaccggaa ccgccgcctt tctcccctgc
     1141 tgagtcgaac gaaggtgtct ctacacgcct tcggccgtga gcagatcgtc aagttgcgcg
     1201 atcaggcttt gtgcgtcctg gaactgaccg atcgcttgcg agagcaagtc tgtatcggcc
     1261 tcggatccgc cggtgagggc ggtcgtgaag ttgagcgcgc cttcgctgag gttcttgttc
     1321 atcgccttgt aggtgtcgtc gatctcgctg accccggccg gagcgtcgta gtccggcagg
     1381 gccatccagg tcgtgataat gttcgcgaga tcggtgtagt cctgatcggt ttcagtttgg
     1441 ctgctaacgc gatcgaggat cgccaatagc ttcgtcatct cggtatcgcg ggcatcgact
     1501 tccttgcgga tgtcggcgag atattccgac ttggtgaggg tgccggattc ctcggttggc
     1561 tgcggctcct cggttgcctt cggagtcttg gtcggcttcg gcgcggcggt cggcgccggg
     1621 ccactgtcaa tgccgagctc atcgccgaca ccaatcgcga tgtcgaccgc agagt
//
LOCUS       DASTMD010000166         1831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_283426, whole genome shotgun sequence.
ACCESSION   DASTMD010000166 DASTMD010000000
VERSION     DASTMD010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283426"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..>1831)
                     /locus_tag="VFQ54_02525"
     CDS             complement(222..>1831)
                     /locus_tag="VFQ54_02525"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEU0163889.1"
                     /translation="LWVGGLASLALWWVIGQRLKSETSTETVPLPLARFSTIALVLFI
                     VAVVTGLANAGFSFPTKTYSDLNDWKLPAVSKLWSSDYGYVLIAKSLVLLLPFGIAAY
                     HHRKIAAAAKSGVVLVASALTKTLRIEALLVLAVALGGSIMALSAPPTISQNALSKVV
                     LTAFAYPATGDPHDLVHLTIDPATQGNNSLAIRLTDNMGKVPADASSDPARIALDFTS
                     LSHGTVRDDVSVSPVDLADATYASEGLDLSFTGWWEIDATIARANQPSATAKFYMILP
                     DPNVHGFGAPPSPKSDEQAQALYADALNRMISLTSLRRDEYIGSGSDALVISDYAWTT
                     GANQRPASFKSSTLYSGGFQTYANGSPPPLPSLDSLSQVAIGGDSWIVGQDGSFTPEQ
                     PILYQPISQWASTFTGAEQFQLGMTQVIDGEKCQIVSFHTPNAPGQSEAWFSWWIGVD
                     SGQMRQMTMVANQHYMIWHNYDFDQDLTIEGPSESQVSPTTPAATVATPSPATPVPNA
                     YANPNVWPGATPAASPAASPVATPASN"
BASE COUNT          308 a          581 c          636 g          306 t
ORIGIN      
        1 ccgttcgcgg ggaggcgagg cgtcgccagc aggcgggctg cgtccccgga actaccaaac
       61 ggtcgtccga gaccatgggc ggggccgggg gacgctgcca ccccccaaac atacaagggc
      121 ggggctttat gccccgccgc aaagcggatt cggcagaaac acgccctgcg ccgaggagcg
      181 ccgaccgcga cccgttctcc ggcccgccct cgtcttcccg gtcagttcga tgcgggtgtg
      241 gccacggggg acgcggcggg actggcggca ggggtagcgc ccggccagac gttcggattg
      301 gcatacgcgt tcggaaccgg cgtcgccggc gatggcgtcg ccacagtcgc ggccggggtg
      361 gtcggcgaga cctgcgactc gctcggtccc tcgatcgtga ggtcctgatc gaagtcgtag
      421 ttgtgccaga tcatgtagtg ctggttggcg accatcgtca tctgccgcat ctggcccgaa
      481 tcgacgccaa tccaccagct gaaccacgct tccgattgcc ccggcgcgtt cggcgtgtgg
      541 aacgagacga tctggcattt ctcgccatcg atcacctgcg tcatccccaa ctggaactgc
      601 tccgcaccgg tgaaggtcga tgcccactgc gagatcggct ggtagaggat cggctgctcc
      661 ggcgtgaacg agccgtcctg tccgacgatc caggagtcac cgccgatcgc aacctgactc
      721 agggagtcga gcgatgggag cggcggtggc gatccgttgg cgtaggtctg gaacccgcca
      781 ctatacagcg tgctcgactt gaacgaggcc ggacgctgat tcgcgcccgt cgtccatgcg
      841 tagtccgaga tcaccagcgc gtccgagccc gacccgatgt attcgtcgcg ccggagggac
      901 gtcagggaga tcatccggtt cagggcatcg gcgtagaggg cctgcgcctg ttcgtcgctc
      961 ttcggggacg gtggggcgcc gaacccgtgc acgttggggt cgggcaggat catgtagaac
     1021 ttcgccgttg cactcggctg gttggctcgc gcgatcgtcg cgtcgatctc ccaccatccc
     1081 gtgaagctga gatcgagccc ttccgatgca taggtcgcat cggcgagatc gaccggcgag
     1141 acgctgacgt cgtcgcgcac cgtgccgtgg ctgaggctcg tgaagtcgag ggcgattcgc
     1201 gccgggtccg aggatgcgtc cgccgggacc ttccccatgt tgtccgtcag acggatcgcg
     1261 agcgagttgt ttccttgcgt cgccggatcg atcgtgaggt ggacgaggtc atggggatcg
     1321 cctgtcgcgg gataggcgaa tgccgtgagc acgaccttgc tcagcgcgtt ctgggagatc
     1381 gtcggcggcg cggagagcgc catgatcgat ccacccaggg ccacggcgag cacgagcagc
     1441 gcctcgatcc gcagcgtctt cgtcagcgca ctggcgacga ggacgacgcc ggacttcgcc
     1501 gccgccgcga tcttgcgatg gtgataggcc gcgatcccga acggcagcag aaggacgagc
     1561 gattttgcga tcaggacgta gccgtagtcg gatgaccaga gcttcgagac tgcgggcagc
     1621 ttccagtcat tgaggtccga gtacgtcttc gtcgggaagg agaagccggc gttcgccagt
     1681 cccgtcacca cggcgacgat gaagaggacg agggcgatcg tcgagaaccg ggccaacggg
     1741 agggggaccg tctctgtcga cgtctcgctc ttcagccgct ggccgatcac ccaccagagc
     1801 gcgagcgagg caagaccgcc gacccacagc g
//
LOCUS       DASTMD010000167         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_104751, whole genome shotgun sequence.
ACCESSION   DASTMD010000167 DASTMD010000000
VERSION     DASTMD010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104751"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..>1678
                     /locus_tag="VFQ54_02530"
     CDS             19..>1678
                     /locus_tag="VFQ54_02530"
                     /inference="COORDINATES: protein motif:HMM:NF020120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEU0163890.1"
                     /translation="MIDDRLTVSTLRTEYLVEPLGIDVLRPRLSWVLSSDRRGVTQTA
                     YEVRVASARAALDRDEGDLWASGRVESGDSARIVYAGSTIRARQRAWWKVRVWDGDGV
                     VSDWSDVTFWERGLSGFSRLSGETPADTWTADWIADPDLRPDPDLPEAVELDALLPAP
                     LFRTSFTVTKEIVRARLYATARGVYEARLNGAKVGDHQLAPGWTDYARRIQYQTFDVT
                     DALRPGENVLGAMVGTGWFAGRVAFAPGARHYGSTPQFLAQLHLDYADGTTEVVATDR
                     SWTTRPGPIRYSDFLMGEYYDAGQERPGWDAPGYDGDGWRPVQLQSKNATPFVSEPAE
                     PVRTQDEFAPAGRTEIEPGVFVFDLGQNIAGWVRLKAAGPAGTTIRLRFAEILNPDGT
                     LYVTNLRSARATDTFVLAGNGLEVFEPRFTWHGFRYVEVSGYPGVPEIEDVTGVFVGS
                     DTRPVGTFTSSDELVNQLQRNIVWGQRGNFLGVPTDCPQRDERLGWLGDAQVFVGTAV
                     GNMDVAGFFTKWMRDVADAQSIAGAFPDVAPRLADLSDGAPAWAD"
BASE COUNT          256 a          538 c          593 g          291 t
ORIGIN      
        1 gaggaaagga tgtttgtcgt gatcgacgac cgcttgaccg tttccacgct ccgcacggag
       61 tacctggttg aacccctcgg gatcgatgtc cttcgcccgc gtctttcctg ggtcctgtcg
      121 tcggaccggc gcggtgtgac gcagacggcg tacgaggtgc gcgtggcgtc ggcccgggcc
      181 gcccttgacc gggatgaagg agatctctgg gcgagcgggc gtgtcgaaag cggggacagc
      241 gcacggatcg tctatgcggg gtcgacgatt cgcgcgcgac agcgcgcctg gtggaaggtc
      301 cgtgtctggg acggcgacgg ggtcgtctcg gactggagcg acgtcacatt ctgggagcgg
      361 gggctctccg gattctctcg cctctctggc gagaccccgg ccgacacgtg gacggcggac
      421 tggatcgccg atcccgacct gcgtccagat cccgaccttc cggaggcggt ggaactcgac
      481 gccctgctgc ccgcgccgct cttccggacg tcgttcacgg tgacgaagga gatcgtccgg
      541 gcccggctct acgcgacggc gcgcggcgtc tacgaggcgc gactcaacgg cgcgaaggtg
      601 ggagatcacc agctggcgcc gggctggacc gattacgcca ggcgcatcca gtaccagacc
      661 ttcgatgtga cggacgcgct ccgaccgggt gagaacgtgc tgggggcgat ggtcgggacc
      721 ggctggttcg cggggcgcgt cgccttcgcg ccgggcgccc gccattatgg gtcgacgccg
      781 cagtttctcg cgcagttgca cctggattac gccgatggca cgaccgaggt cgtcgcgacc
      841 gatcgctcgt ggacgacccg gcccggtccg attcgctatt ccgatttcct gatgggcgag
      901 tactacgatg ccggccagga gcgtccgggt tgggatgctc ccggctacga cggtgatggc
      961 tggcgtcccg tccagctgca gagcaagaac gcaaccccgt tcgtcagcga gccggcggag
     1021 ccggttcgca cacaggacga gttcgcgccc gccggccgga ccgagatcga acccggtgtc
     1081 ttcgtcttcg acctcggcca gaacatcgcc ggatgggtgc ggctcaaggc ggccggtcca
     1141 gcgggaacga cgatccggct gcggttcgcc gagatcctga atccggacgg caccctctac
     1201 gtcacgaatc tccggagtgc gcgcgccacc gataccttcg tccttgcggg aaacggcctc
     1261 gaggtcttcg agccgcgctt cacctggcat ggctttcgat acgtcgaagt cagcggatat
     1321 ccaggggttc cggaaatcga ggatgtgacc ggggtcttcg ttggctcgga tacacgaccg
     1381 gtcggcacgt tcaccagttc cgatgaactc gtgaaccagt tgcagcgaaa catcgtctgg
     1441 ggccagcgcg ggaacttcct cggcgtgccg actgactgcc cgcagcgcga cgagcgactc
     1501 ggctggctcg gcgacgccca ggtcttcgtc gggacggcgg tcgggaacat ggatgtcgcc
     1561 ggattcttca cgaagtggat gcgtgacgta gccgacgcgc agtcgatcgc cggggcattc
     1621 ccggatgtcg cgccgcgtct ggccgatctc tcggacgggg cgcccgcctg ggcggact
//
LOCUS       DASTMD010000168         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2693069, whole genome shotgun sequence.
ACCESSION   DASTMD010000168 DASTMD010000000
VERSION     DASTMD010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2693069"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /gene="cydB"
                     /locus_tag="VFQ54_02535"
     CDS             complement(<1..969)
                     /gene="cydB"
                     /locus_tag="VFQ54_02535"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEU0163891.1"
                     /translation="MLPTVWFILIAVLWIGYLFLEGFDLGVGMHLLFFTREEREKRVM
                     LNAIGPVWDGNEVWLITAGGATFAAFPDWYASLFSALYIPLVLVLVGLIARAVAIEYR
                     GKGTSARWRRGWDLALGLGSFVAAFGVGAMLALTTTGFPLDANGDRVGGAFAWATPWA
                     FIGGFAVVGFSLVHASLFLALKTDDAVRVRSNRFVARVLPFALIPIVVWVVGIQLATG
                     TGITWAITLVAAAFAVAAWIANRRGREGRAFVFLGAFLLAGVAAIFGAVYPVVLPSTL
                     SHAFDLTVANASSAPYTLSVMSVVACFGIPLVLAYQGWTYWVFRKRI"
     gene            complement(978..>2142)
                     /locus_tag="VFQ54_02540"
     CDS             complement(978..>2142)
                     /locus_tag="VFQ54_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU0163892.1"
                     /translation="AYFIIVANSWMQHPVGVELVNGHPVMTDVWAVLTNNTAIVAFIH
                     TIFGSFGVGGGFLLGISWYQLWQRRRDGIDTIDADKRVVVGEAPEIPGRDKDDHRVWI
                     TSLRIGAVVAIVAFGGVALSGDAQAKLMFQQQPLKMAAAEGACHTGSSFSVLSIGDPG
                     SRDCSNVQGIIEIPGILSFLANGNFTTDVKGINELIPEYQAKYGTTLPDNPLYGARAG
                     EKIEYVPIMEVTYWGFRLMIGFGILAAAAALLALWLTRRGTVPRPRWLMQLAIASIIA
                     PFAANSAGWIFTEMGRQPFVVAPNPTGVDGVYMFTAAAVSPGVSFGEILFSVITFTVV
                     YAVAMVFEIRLLVSYVRGGVASALPELAHPAPSGGADPDDPDAGSDDVLAFAY"
BASE COUNT          420 a          685 c          740 g          297 t
ORIGIN      
        1 gatgcgcttg cggaacaccc agtacgtcca cccctggtac gcgagcacga gcgggatgcc
       61 gaagcaggcc acgaccgaca tcacactgag cgtgtagggc gccgatgacg cgttcgcgac
      121 ggtgaggtcg aaggcgtggc tgagggtcga ggggaggacc accggataga cggcgccgaa
      181 gatcgcggcg accccggcga gcaggaacgc cccgaggaac acgaaggcgc gaccctcccg
      241 gccacggcgg ttggcgatcc aggcggccac ggcgaacgct gcggcgacga gagtgatcgc
      301 ccaggtgatg ccggttccgg tcgcgagttg gatccccacg acccagacga cgatcggaat
      361 gagcgcgaag ggaagaactc tcgcgacgaa tcggttgctg cgcacgcgca ccgcgtcatc
      421 cgtcttgagg gcgaggaaga gcgacgcgtg aacgaggctg aacccgacga cggcgaagcc
      481 tccgatgaat gcccagggtg tcgcccaggc gaacgcgccg ccgacccggt caccattggc
      541 gtcgagtgga aagcccgtgg tcgtgagagc gagcattgca ccgaccccga aggcggccac
      601 gaacgagccg aggcccagtg cgagatccca tccgcggcgc cagcgtgcgc tcgtgccctt
      661 gccccggtat tcgatggcga cggcgcgcgc gatcaatccg acgagcacga gcacgagtgg
      721 gatgtagagg gcggagaaca gcgaggcgta ccaatcgggg aacgcggcga acgtcgcgcc
      781 gcccgcggtg atgagccaga cctcgttgcc gtcccagacg gggccgatcg cgttgagcat
      841 gacgcgcttc tcgcgctcct cccgcgtgaa gaacagcaga tgcatgccga cgccgaggtc
      901 gaagccctcg aggaacaggt agccgatcca gaggacggcg atgaggatga accagaccgt
      961 gggaagcatt gtcactccta atacgcgaag gcaaggacgt cgtcggatcc ggcgtccggg
     1021 tcatccggat cggctccgcc tgacggcgct gggtgggcga gttcgggcag cgcactcgcg
     1081 acgccgccgc gcacgtagct caccagcagc cggatctcga agaccatggc gacggcgtag
     1141 acgacggtga aggtgatgac ggagaagagg atctcgccga aactgacgcc cggcgagacc
     1201 gcggccgcgg tgaacatgta gacaccgtcg acgcccgtgg gattcggcgc gacgacgaag
     1261 ggctggcgtc ccatctcggt gaagatccag ccggccgagt tcgccgcgaa cggagcgatg
     1321 atgctcgcta tcgcgagctg catgagccag cgcggccgcg ggacggttcc ccggcgcgtg
     1381 agccagaggg ccaggagagc ggcggccgcc gccaggatgc cgaacccgat catcaaccgg
     1441 aagccccagt acgtgacctc catgatcggc acgtactcga tcttctcgcc agcgcgggcg
     1501 ccgtacagcg ggttgtcggg caacgtcgtg ccgtacttgg cctggtactc ggggatcagt
     1561 tcgttgatcc ccttcacatc ggtcgtgaag ttgccgttcg cgaggaacga gaggatcccg
     1621 ggaatctcga tgatgccctg tacgttgctg cagtcgcgtg agcccgggtc gccgatcgac
     1681 aagaccgaga agctcgatcc cgtgtggcag gcgccctcgg ccgcggccat cttgagcggc
     1741 tgctgctgga acatcagttt cgcctgggcg tcacccgaca acgcgacacc gccgaacgcg
     1801 actatcgcga cgaccgcgcc gatgcgcagc gacgtgatcc acacgcggtg atcgtccttg
     1861 tcgcgaccgg ggatctcggg cgcttcgccg accaccacgc gcttgtccgc gtcgatcgtg
     1921 tcgatcccgt ccctgcggcg ctgccacagc tgataccagg agatgccgag aaggaacccg
     1981 ccaccgacgc cgaacgaacc gaagatcgtg tggatgaacg cgacgatggc agtgttgttc
     2041 gtcagcacgg cccagacatc cgtcatcacg ggatggccgt tgaccaattc cacgccgacg
     2101 ggatgctgca tccacgagtt cgcgacgatg atgaagtacg cg
//
LOCUS       DASTMD010000169         1878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_729491, whole genome shotgun sequence.
ACCESSION   DASTMD010000169 DASTMD010000000
VERSION     DASTMD010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729491"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1140
                     /locus_tag="VFQ54_02545"
     CDS             97..1140
                     /locus_tag="VFQ54_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEU0163893.1"
                     /translation="MRVTVVGAGAIGGTTGVLLHQAGHDVTLVDRDAEHVAKINRDGF
                     RISGLVETTERVPAVTASELPSFIDRTGPLGMVVLAVKAMDTEAAATQIRPYLAPDGF
                     IVSYQNGLNEETIAKIVGPERTIGAFVHFGADLVEPGHVVLGTRPRTYISELDGRETP
                     RIHEVAGVLSAVTEVEVTANLEGYLWGKLCYAALAFVVSSVDAPIDEVVMSPDARIAI
                     RGAVAEVVDVARAQGIQLEDIHGFQPSLFDRHNPDLITDTDALFERWAEEGRMAIKRH
                     MGIHRDIKVRKRRTEVDFQVAPVSQRGRTAGVPTPVTDQLVELIHQIEAGTRPQDWSA
                     IVELSRLPAVPSA"
     gene            complement(1147..>1878)
                     /locus_tag="VFQ54_02550"
     CDS             complement(1147..>1878)
                     /locus_tag="VFQ54_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0163894.1"
                     /translation="PRFVESTPSSTGLSLLDLPAQLREHGYDALQLCHFHLPSRSASY
                     LAELRESLAGSRIELDALLVDDGDLTSDKDGDLVEGWIGEWLDTAATLGARRVRLSAG
                     RSTPSAETVQESATRLARLAANHPEVRVITENWQELTPDAGTVLSLLELAGDGVGLMI
                     DLGNWSGPDKYAELARIAPLAESCHAKCHFTGSVADGEDYLASLAILKESGYDGPLAL
                     IYDGRDDDEWTKLDVEYEMVTRVFA"
BASE COUNT          352 a          583 c          615 g          328 t
ORIGIN      
        1 atcgtacgtg gggcggtttt ttcgtctttc gctatggacg gcgacaatcg tcacggccat
       61 ttcggcgcga gtgggactca tcctgaaagg aacaagatgc gcgtaacagt ggttggggca
      121 ggagcgatcg ggggaaccac cggtgttctc ctccaccagg caggacacga tgtcaccctg
      181 gtggatcgtg acgccgaaca cgtcgcgaag atcaatcgcg acggattccg catcagcggt
      241 ctcgtcgaaa cgacggaacg cgtacccgcg gttacggcct cggagttgcc cagcttcatc
      301 gatcgaacgg gcccgctcgg catggttgtg ctcgcggtca aagcgatgga caccgaggcg
      361 gcggctacgc agatccggcc gtatcttgcc ccggacggct tcatcgtctc ctatcagaat
      421 ggattgaacg aggagacgat cgcaaagatc gtcggtcctg agcgaacgat tggcgcgttc
      481 gttcatttcg gagccgacct cgtcgaaccc gggcatgtcg ttctcggaac ccgccccagg
      541 acctacatca gcgagctcga cgggagggag acgccacgga tccacgaagt cgccggcgtc
      601 ctctcggcgg tgacagaggt ggaggttacc gccaacctgg aagggtacct ctgggggaaa
      661 ctctgctatg ccgcgctggc gttcgtcgtc tcctccgtcg acgcgccgat cgacgaggtt
      721 gtgatgtcac ccgacgcccg cattgcgatc cggggggcag tcgcggaggt cgtcgacgtc
      781 gcccgggcgc agggaatcca gctggaggat attcacggct tccagccgtc cctcttcgat
      841 cgccacaatc ccgacctgat taccgacacc gacgccctgt tcgaacgatg ggcagaggaa
      901 ggcagaatgg cgatcaagcg acacatgggt atccatcggg atatcaaggt ccgcaagcgc
      961 cggacggaag tcgacttcca ggtcgctccc gtcagccagc gcgggaggac cgcgggcgtg
     1021 ccgacaccgg tcaccgatca gctcgtcgag ctgatccacc agatcgaagc cgggacgcgc
     1081 ccgcaggact ggtccgccat cgttgagctg agcaggctcc cagcggttcc gtcggcatag
     1141 gcgccgttac gcgaagaccc gggtaaccat ctcgtactcg acgtcgagct tggtccattc
     1201 gtcatcgtcg cgaccatcgt agatgagcgc aagcggaccg tcgtatccgc tttccttcag
     1261 gattgccagc gacgccagat agtcttcgcc atcggcgacc gatccagtga agtgacactt
     1321 tgcatgacac gattcggcga gcggtgcgat gcgggcgagt tccgcatact tgtcgggacc
     1381 gctccagttt ccgagatcga tcatgaggcc gaccccatcg ccggccagct cgaggagaga
     1441 gagcacggtc ccggcgtccg gcgtcagctc ctgccagttc tccgtgatga cgcgtacttc
     1501 cgggtgattc gcggcaagcc gggcgaggcg ggttgcgctt tcctggaccg tctccgccga
     1561 gggcgtcgag cgcccggcgc tcaggcgcac ccgccgggca ccgagcgtgg cagccgtgtc
     1621 caaccactcc ccaatccagc cttcgacgag gtcgccatcc ttgtcgctcg tgagatcgcc
     1681 gtcgtcgacg aggagtgcgt cgagttcgat ccgggacccc gcgagcgact cgcgtagctc
     1741 tgcgagatag gaagcggagc gcgagggcag gtggaaatga cagagctgaa gtgcgtcgta
     1801 cccgtgctcc cgcagttgag cgggaaggtc gagaagcgag aggccagtcg aggacggtgt
     1861 ggactcgacg aaacgcgg
//
LOCUS       DASTMD010000170         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_462184, whole genome shotgun sequence.
ACCESSION   DASTMD010000170 DASTMD010000000
VERSION     DASTMD010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462184"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFQ54_02555"
     CDS             <1..343
                     /locus_tag="VFQ54_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0163895.1"
                     /translation="SRHVATHAPQIEIRRLDGGMLPSYTPVEHPLAEVVRGAVEAGFG
                     QRPIDIPLVGGSLPDATWTKTLGIPSFLVPYGAPEQANHSPNESYRIERLWQGIATSA
                     TLLALLSAAEI"
     gene            complement(589..1074)
                     /locus_tag="VFQ54_02560"
     CDS             complement(589..1074)
                     /locus_tag="VFQ54_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163896.1"
                     /translation="MVKALTTDVYFMKEPQPGALIEATAVAFGVPVESVVVAPVVSET
                     GRVSVKDPAVAVLWRREVEDQGGDFPIRFNLMIPEEERGMIFDRLDGVETHLQIPFIT
                     DAENDYELRLYLPNGEVLVIPDTMSDDDEYEMHLTPAQLDAINRASIEAGARPRVSIA
                     S"
     gene            complement(1052..1456)
                     /locus_tag="VFQ54_02565"
     CDS             complement(1052..1456)
                     /locus_tag="VFQ54_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163897.1"
                     /translation="MKAPAARHYINRITAKTAVKEVTTLIDPGIPVEDDMRGIRGGRA
                     IRDGDRFTINGRTYQQEPNGTMFPVDGDGLIPRVPRGVMNALLAFARYNEINERSEFE
                     IASREISDEDVEMAREIWRRRERYRDGQGTDN"
     gene            complement(1638..>2384)
                     /locus_tag="VFQ54_02570"
     CDS             complement(1638..>2384)
                     /locus_tag="VFQ54_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0163898.1"
                     /translation="EYGFRSIKFKGGVLPPDDEIATVFAMREAFGPEMPIRIDPNCGW
                     SVETSIRVGTELNGTLEYFEDPTGEIDGMAAVRKGLLEKGIDLPLATNMCVTSFDDVA
                     EAVAKDAVQVILGDHHYWGGLKAVVELGRICTTFGLGLSMHSNSHLGVSLMAMAHLAV
                     ATPGLTYDADTHYPWLHAEDEILVGGKLQFVGGTIPVTGAPGLGIEVDRDALARGNER
                     FERIPYRDRDDVGYMRRTVDPTWEKLLPRW"
BASE COUNT          432 a          791 c          712 g          449 t
ORIGIN      
        1 ctcccgccac gtcgcgacgc acgcgccgca gatcgagatc aggcggctcg acggcggcat
       61 gctcccgtcc tacacgccgg tcgaacaccc gctcgcagag gtcgtgcgtg gcgcggtcga
      121 ggctggcttc ggccagcgcc cgatcgacat cccgctcgtg ggcggcagcc tcccggacgc
      181 gacgtggacc aagacgctcg gcatcccgtc cttcctcgtg ccatacggcg caccggagca
      241 ggcgaatcac tcccccaacg agagctaccg catcgagcgt ctctggcagg gcatcgccac
      301 ctcggccacc cttctcgccc tcctctccgc cgcggagatc taaagcgaga tctggaaccg
      361 ccgacacgtt tggtgccgcc ggggttggta gggcggggtg ttataccccg ccgaaaaagc
      421 ggattcgggg gaagcacgcc ccgccccagg gtggctatcg ccgcgggcct ctggatggtg
      481 attcgatccc gtgggctatc gaaaaatcac cgcacaggga tcgaccgctc ccgaccgtca
      541 gcgtcaagaa tctgtgccga tggtccggtc gccgtacaag agggcaggtt agcttgcgat
      601 ggagacccgg ggacgagctc cggcctctat cgaggctcga ttgatcgcat caagttgtgc
      661 gggggtaagg tgcatctcgt actcgtcgtc gtcggacatc gtatccggga tgacgagtac
      721 ctctccgtta gggagataga ggcggagttc atagtcattc tcggcgtccg tgatgaaggg
      781 aatctgcaaa tgggtctcga cgccgtccag gcgatcaaag atcattccac gttcctcttc
      841 cgggatcatg aggttgaacc ggatcggaaa atcgccgccc tgatcttcaa cctcacgccg
      901 ccagagcacg gcaacggctg gatccttgac ggagacgcgt ccagtttcac tgacaacagg
      961 tgcgacgacc acggattcga cgggcacgcc gaatgcaacc gccgtggctt caataagagc
     1021 gccgggttgt ggctctttca tgaaatagac gtcagttgtc agtgccttga ccatcgcgat
     1081 accgctctct cctgcgccag atctctcgcg ccatctcgac atcctcgtcc gagatctcac
     1141 gactcgcgat ctcgaattcg gaccgttcat tgatttcatt gtaccgggcg aaggcaagca
     1201 gtgcgttcat cacaccccgc gggacgcggg gaatgaggcc gtcaccatcg accggaaaca
     1261 tggttccgtt gggctcttgc tgataggtgc gaccgttgat cgtgaacctg tcgccatccc
     1321 ggatggcccg tccgccccga ataccgcgca tgtcatcttc gaccgggata cccggatcga
     1381 tcagggtcgt cacttccttc accgcggtct ttgctgtgat ccggttgata tagtgccgag
     1441 cggccggcgc cttcacggga cgcttcggct tgttttgagc gacctcctcg ccttgctctt
     1501 ccagtgcccg cctccccaca gatgaccggc cggatcgccc ccctgtcgct cacgagatat
     1561 tcaggttcaa tcttgccatc gttgaaggca ccgaacgcgt ccttcgacca gattgcgggc
     1621 ggctgagggc agggaaccta ccaccgcggg aggagcttct cccaggtcgg gtcgacagta
     1681 cggcgcatgt agccgacgtc gtcgcgatcg cggtacggga tccgctcgaa acgctcgttg
     1741 ccacgcgcca gcgcgtcgcg atcgacctcg ataccgagtc cgggcgcacc agtcaccggg
     1801 atcgtccctc ccacgaactg caacttgccg ccaacgagga tctcgtcctc ggcgtgcagc
     1861 cacgggtaat gagtgtcggc gtcgtaggtg aggccgggcg tcgcgacggc gaggtgggcc
     1921 atcgccatca gggagacgcc gaggtgggag ttcgagtgca tgctgaggcc gagcccgaac
     1981 gtcgtgcaga tgcggccaag ttccaccacc gccttcaatc caccccagta atgatgatcg
     2041 ccaaggatca cctgcacggc atccttcgcc accgcctcgg cgacatcgtc gaagctcgtg
     2101 acgcacatgt tggtcgccag cggcagatcg atacccttct cgagaaggcc cttgcgaacc
     2161 gccgccatcc cgtcgatctc acccgtcggg tcctcgaaat actcgagtgt cccgttgagc
     2221 tcggttccga cgcgaatcga ggtctcgacc gaccacccgc agttcggatc gatccggatc
     2281 ggcatctccg gaccgaaggc ttcccgcatc gcgaagaccg tcgcgatctc gtcgtccggc
     2341 gggaggactc cccccttgaa cttgatgctc ctgaagccgt actc
//
LOCUS       DASTMD010000171         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1086913, whole genome shotgun sequence.
ACCESSION   DASTMD010000171 DASTMD010000000
VERSION     DASTMD010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086913"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1985)
                     /locus_tag="VFQ54_02575"
     CDS             complement(<1..1985)
                     /locus_tag="VFQ54_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163899.1"
                     /translation="MVRTRRGRTTRLVVMAFALLGLILNAGNIAAAQEASPVVPTPTV
                     ASTSTSTATQAPTATATTAPTATSAIQGQATDTVNVTVRLWVCPDNSGATVDYFYDAA
                     SSAPDRSGANCDLLPSNGIPDLPVYLLDASENVIASTQTNGGIATFQNIAPGSYSVAS
                     SVVPLDFEFDATIAADTTPLVDVIYTGDAIASTATALPVEYLTCENAAKAGSYDFLIQ
                     TLDLGISSTSSCVPGPLATDGTTAVQLNGATTYGPSALTPSGSAYFFPITPGDYTATV
                     LVGGSPTATSDSFNISSTYFTPSELQIIHYVTPDTIPTPTPSVGSTGDVAVLTSYCDD
                     PSREGEIDFLINAGYFSPASTTDCQAAFVTSTQLQLINTTTNDVYEPQGDYYYYEGSA
                     FPDLPPGTYVLKDVASGTTSDPFTVSGGSTTYIQMNIYAAAPQLGLVLIAPFTCLDPS
                     RAGQTEYTILTDYGYAESSTSNCEEIYGSNVTPEIYTLTNTETGDTYPTSSYDTPTGE
                     GYAVVPAGTYTVTNESGAVSDQFQVTASSYYYYANLTVVIAKNFVGDDEFTPVPTANP
                     EDENSAYFNLMPYLCTTNDPDKVGAEFLIASDVYTENGAAAVPTVAAPTQNCAPGTTQ
                     PFQFEAIPVGGGDPIPLELCACSSYYAYTLPDIYVAPG"
     gene            complement(2374..>3178)
                     /locus_tag="VFQ54_02580"
     CDS             complement(2374..>3178)
                     /locus_tag="VFQ54_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163900.1"
                     /translation="LSDDYTFGFFMLSSLLPTPTPTATLVPTETPTATATTTAIATEP
                     ATSTATTAPSETAIPTGTTASTATATITAVPTGSPVDVTGADITLSTADGGAIPDDGQ
                     ICVGDLCQSTGATASAASFSAAAASGSAYSFTGLPAGTLPITVTNVAPYADFTGTITI
                     SSGEITSQAVTLSLIPVTVAPTTTTTTQASPVVNPTEPPVTATTIPATTVPATTVSPT
                     KAAGTPGVKSLPNTGQGTAGRDNSLALVLFGLASLLLAVVAIGARRRRA"
BASE COUNT          551 a          871 c         1125 g          631 t
ORIGIN      
        1 ccaggggcga cgtagatatc agggagggtg taagcgtaat aggaactaca cgcgcagagc
       61 tcgagcggga tcggatcgcc gccaccgacg gggatcgctt cgaactggaa tggttgggtc
      121 gtcccgggcg cgcagttctg cgtgggtgcg gctaccgtcg gcaccgcggc agcgccattc
      181 tccgtgtaga catcgctggc gataaggaac tcggcaccga ccttgtcagg atcgttcgtg
      241 gtgcagagat agggcatcag attgaagtag gcgctgttct catcctcggg gttcgcggtc
      301 gggacggggg tgaactcgtc gtcgccaacg aagttcttgg cgatgacgac ggtcagattc
      361 gcatagtagt agtagctgct cgccgtgacc tggaactgat cggaaacggc gccactctcg
      421 tttgtcaccg tataggttcc ggctggcacg accgcgtagc cctcgcccgt tggcgtgtcg
      481 tagctgctcg tcggataggt atcgcccgtc tcggtattcg tgagcgtata gatctccggg
      541 gttacgttcg agccgtagat ttcctcacag ttcgaggtgc tcgactcggc gtagccgtag
      601 tcggtcagga tcgtatattc ggtctggccg gctcgcgatg ggtcgagaca ggtgaacgga
      661 gcgatcagaa ccagaccgag ctgtggcgct gccgcgtaga tgttcatctg gatgtaggtc
      721 gtgctgccgc cactgacagt aaaggggtcc gaggtcgtgc cggaggcgac atccttcaac
      781 acgtatgtgc cgggcgggag gtcagggaag gcgctccctt cgtagtagta gtaatcgccc
      841 tggggctcgt agacgtcgtt cgtcgtcgtg ttgatcagct gcagctgcgt cgaggtgaca
      901 aatgcagcct ggcaatccgt cgttgatgca ggggagaagt accccgcgtt gataaggaag
      961 tcgatctcgc cctcgcggct cggatcgtca caataggatg tcagcacggc gacatccccg
     1021 gttgatccca ccgacggggt tggtgtggga atggtgtcgg gggtgacgta atggatgatc
     1081 tgcaactcgg acggcgtgaa ataggtagaa gaaatgttga acgagtccga cgtcgcggtc
     1141 gggctgccgc caacgaggac tgttgcggtg tagtcgccgg gcgtgatcgg gaagaagtat
     1201 gccgagccgg acggcgtgag cgcggacggc ccatacgtcg tcgcgccgtt caattgaacc
     1261 gcagtcgttc catcggtcgc cagggggccc ggcacacacg acgaggtact ggagatgccg
     1321 aggtcgagcg tctggatcag gaagtcatag ctccctgcct tggccgcgtt ttcgcacgtc
     1381 aggtactcga cgggtaaggc agtcgccgtc gaggcgatgg cgtcgccggt atagatgacg
     1441 tcgacgagcg gcgtcgtatc ggcggcaatc gtcgcgtcga actcgaagtc gaggggcacg
     1501 acgctggacg caacggaata gctacccggc gcgatgttct ggaaggtggc aatgccgccg
     1561 ttcgtctgcg tgcttgcgat cacattctcg gatgcatcca gcaggtagac cggaaggtct
     1621 ggtattccat tggacggcaa aagatcgcaa ttggcaccgg agcggtcggg tgccgaggag
     1681 gcggcgtcgt agaaataatc aacggtcgcg ccactgttgt ccgggcagac ccagaggcgg
     1741 acggtgacgt tgacggtgtc ggtggcctgg ccctggattg cggaggtggc cgtaggcgcc
     1801 gtcgtagccg tagccgttgg cgcttgtgtc gcggtggatg ttgacgttga cgccacggtc
     1861 ggcgtcggca cgaccggcga cgcttcttgt gcggcagcga tgttgcccgc gttaaggatc
     1921 aaccccagta gcgcgaacgc catcacaacg agacgcgtcg tgcgccctct ccgagtgcga
     1981 accatgccga tcttctcccc ccgattactc acgactgtcg atgaacaaac tccgccgtgg
     2041 caatgttctc taatgtaaac atgtcccact tgagaaatat cacgttcaat ggaagtggtc
     2101 aagagtgtga ctgatatgaa cggtcatgct tcgcgagacg gtcgtcggcc gcgtctggtg
     2161 accctggaat actgtccctc gcagtcaccg tcagaggttg ggttttgtgt ggcggcacaa
     2221 tggtattggc cgttccgcgc tcgtcctccc gagcggttcg ggtcgctccg atcatatcct
     2281 cccggccgct attccgtccg aacgtgagga gcccgcgtcc tgaccgggac gcgggctcca
     2341 ttaacgtgac gaagacgaat aggttgcgtg ggcttacgcg cggcggcggc gagcaccgat
     2401 cgcgacgacg gccaggagca gacttgccag accgaagagc accagtgcca gcgaattatc
     2461 gcggcccgca gtgccctgac cggtgttcgg cagggacttg acccctggtg ttcccgcggc
     2521 cttcgtcggc gaaaccgtcg ttgccgggac ggtcgtggcc gggatggtcg tcgcggtgac
     2581 cggaggctcc gtcgggttca cgaccggcga ggcctgcgtc gtggtcgtgg ttgtcggcgc
     2641 gaccgtgacc ggaatcaggc tgagggtgac cgcctggctc gtgatctcac cgctgctgat
     2701 cgtgatcgtg ccggtgaagt cggcgtacgg agcgacgttg gttaccgtga ttggcagcgt
     2761 tccggcaggg aggccggtga agctgtaggc ggacccggaa gcagctgcgg cgctgaagga
     2821 ggcggcggag gcggtcgcgc cggttgactg gcagagatcg ccgacgcaga tctggccgtc
     2881 atccgggatc gcgccgccat cggcggtgga gagcgtgatg tcggctccgg tcacatccac
     2941 tggcgagccg gtgggaaccg cggttatggt cgccgttgcc gtggaggccg tcgtgccggt
     3001 cgggatcgcg gtctcactcg gtgcggtggt tgcggtcgat gttgctggct ccgtcgcgat
     3061 cgccgtggtt gttgccgtgg ccgttggcgt ctccgtcggg accaatgtgg cggttggcgt
     3121 cggggttggc agcagcgacg acaacatgaa gaagccgaag gtgtagtcgt ccgagagg
//
LOCUS       DASTMD010000172         1970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_373411, whole genome shotgun sequence.
ACCESSION   DASTMD010000172 DASTMD010000000
VERSION     DASTMD010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373411"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFQ54_02585"
     CDS             <1..769
                     /locus_tag="VFQ54_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU0163901.1"
                     /translation="ATVIAFIVPGNVTSFPDDPFSGPDFSLVGDFSFGFFSKIGFLSS
                     VLAILSLMLSDFFDTMGTLIGVGSRAGYLDENGQLPDAEKPLLVDSVAAIAGGVVSSS
                     SATSYIESAAGVSVGGRTGLVVVTTGALFLLALPFVALVNAIPGVATAPALIVVGFLM
                     TGVLSEQESVGADGRIQKTKAINFGDIEEGLPVILTMLLMPFTMSITNGIGAGFITYV
                     LVKIARGKARLVHPAVYVVALAFLVYFLRWALFDAKF"
     gene            826..1794
                     /locus_tag="VFQ54_02590"
     CDS             826..1794
                     /locus_tag="VFQ54_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEU0163902.1"
                     /translation="MPDVSKIVVAMGDENGLADGLRAAHPNLTIVGAGDKDDILREIV
                     DADGVICWRIPNESLDVAERLKWVHVAAAGVEALLAMPGFREKDLLLTNSSGVSAPNM
                     AEHAIAMILAFGRRFPALIRSQGRHAWREWDSFGGAFEIGGQTVALIGLGAIGSETAK
                     RAKALGMHVVGVKRTAAGELPNVDEIHAIDDIDTALAQADHVVSSLPSTSETRGLFDA
                     GRFAAMKEGSFFYNIGRGTTVVQDELIAVLKSGHIAGAGLDVTDPEPLPAESPLWDLE
                     NVLITSHTSGNSPRVRERVLELAIENVRRFVAGEELLNIVDQSLGY"
BASE COUNT          331 a          581 c          644 g          414 t
ORIGIN      
        1 ggcgaccgtc atcgccttca tcgtgccggg gaacgtgacg agctttccgg acgatccgtt
       61 ttccggcccg gacttctcgc tcgtcggcga cttcagcttc gggttcttct cgaagatcgg
      121 attcctctca tccgtgctcg cgatcctgag cctgatgctc tcggatttct tcgacacgat
      181 gggaaccctg atcggcgtcg ggtcgcgggc cgggtacctc gacgagaacg gccagctgcc
      241 cgacgccgag aagccgctcc tggtcgacag tgtcgccgcg atcgccggcg gagtcgtcag
      301 ttcatcgtcg gcgacgtcgt acatcgagtc agcggccggt gtcagtgtcg gcggtcgaac
      361 cggtctcgtg gtggtcacca ccggcgcact cttcctcctg gcgctgccgt tcgtcgccct
      421 cgtcaatgcg atcccgggcg tcgcgaccgc accggccctg atcgtcgtcg gctttttgat
      481 gacgggtgtg ctctccgaac aggagagcgt cggggcggac gggcgcatac agaagacgaa
      541 ggcgatcaac ttcggtgaca tcgaagaggg attgccggtc atcctgacga tgttgctgat
      601 gccgtttacc atgagtatca cgaacgggat cggcgccggg ttcatcacct atgtgcttgt
      661 caagatcgcg cgcggcaagg cgcgtctggt ccatcccgcc gtgtatgtcg ttgccctcgc
      721 attccttgtc tacttcctgc ggtgggcgct cttcgacgcc aagttctgac gtgttcgcca
      781 ccctctccgt ctcggttggg tgattcagtg aagggtgacg gaaccatgcc ggatgtctcg
      841 aagattgttg tggcgatggg agacgagaac ggattggcgg acggacttcg tgccgcgcat
      901 ccgaacctta cgatcgtggg agcgggggac aaggacgaca tcctccgtga gatcgttgac
      961 gccgacggcg tgatctgctg gcggattccg aacgaatcac tcgatgtcgc cgagcggctg
     1021 aagtgggttc atgtcgccgc cgccggtgtg gaagcgctcc ttgcgatgcc agggtttcgc
     1081 gagaaggacc tgcttctcac gaacagcagc ggggtcagcg cgccgaacat ggcggagcac
     1141 gcgattgcga tgatccttgc cttcgggcga cgtttcccag cgctgatccg ctcccagggg
     1201 cgtcacgcct ggcgggagtg ggattccttt ggcggcgcgt tcgagatcgg cggacagacc
     1261 gtcgcattga ttggtctcgg cgcgatcggc tccgagacgg cgaagcgggc gaaggccctc
     1321 ggcatgcacg ttgttggcgt caagcgcacg gcggccggcg aactgccgaa cgtcgatgag
     1381 atccatgcaa tcgacgatat cgataccgct ctcgctcagg ccgaccatgt cgtgagcagt
     1441 ctcccatcca cgagcgagac gcgcggcctc ttcgatgccg gtcgcttcgc cgcgatgaaa
     1501 gaagggagct tcttctacaa cattggtcgc ggtacgacgg tggtgcagga cgagttgatc
     1561 gcggtgttga aatccggtca catcgctggc gccggtctcg acgtcacgga cccggagccg
     1621 ctccccgcgg agtctccgct gtgggatctc gagaacgttc tgatcacctc ccacacgtcc
     1681 ggaaattccc ccagggtgcg ggagcgcgtg ctggagctcg cgatcgagaa cgtccgccgt
     1741 ttcgttgcgg gcgaagaact tctcaacatc gtcgaccagt ccctgggtta ttaggtcggt
     1801 gccaccgcgg gagtggttcg atttcgagga atttgaaggt tttcagatcc ggatgcggtt
     1861 cgagcttggc gagacgcatg gaaagaatat cgacaagttc ctgtggcagc tggagattga
     1921 aatcgagggt cgctggtatc ctgtcctgcg atttgacacc gagcattacg
//
LOCUS       DASTMD010000173         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1712548, whole genome shotgun sequence.
ACCESSION   DASTMD010000173 DASTMD010000000
VERSION     DASTMD010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712548"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(518..769)
                     /locus_tag="VFQ54_02595"
     CDS             complement(518..769)
                     /locus_tag="VFQ54_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163903.1"
                     /translation="MTGSAVAGAQEATPGATPIATTTPTTVAPTTAAGAPTAAVVALP
                     NTGQGSSSSNNGSIILLLGAMTLVAGAAAYSWQQRRSNN"
     gene            complement(1365..>2075)
                     /locus_tag="VFQ54_02600"
     CDS             complement(1365..>2075)
                     /locus_tag="VFQ54_02600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU0163904.1"
                     /translation="EVVDALPAHHLVVDGDEFRERFVTWDEAAAWFGTTLGPLSAEVG
                     DSRVVNALGSQGVVPGALPDGAILEVSPRAAGWIREVAQGIERGYALIVDYGYPAAEL
                     YRGHRLGGLLRGYREHTVTDDPFTAIGEQDLTTHVDFSWLASAARGAGMHEIGLTTQA
                     DFLAALGLGDLLVTFQHDGETSIDEYYQAQAAVLRLIDPGGMGRFRVLGLSKGVTNAD
                     GVHPVRGFVAPALDYPEF"
BASE COUNT          403 a          643 c          645 g          384 t
ORIGIN      
        1 ccacgttggg cgatgacgcg caacgtccgg tcgcgttcct gaacttcgga cacctgatcc
       61 gactggcgtt acgccagccg gcgcatcatg cccacaatct cgcacgtcca ccgggatacc
      121 gatggcttcc ctgcccggga ctgactgtgt gcatggcatc ctgacatcga tcaataccag
      181 caaggcgatc tcgttatcaa gaaaatgacg gaatcccccg tcagatgagg tgatcggtct
      241 ggtactggaa cgatgcccag agcgtaggcc ggatattccg gaaagtcaac gcgcgaggcg
      301 gcggcgatga cctgaagaag tcatgtgagg ggcgattgga cccggtcagg atgacggatg
      361 tgccgtccaa agacatggcg tcgcgttcgt ctcatgggga gagcgaacgc gacaggcgaa
      421 agacaagaac ggacccctga ttgtgtcagg ggtccgttct tgtgggtgat ctttggttgg
      481 atgcgccggt tagttccggc gcatccatcc gaacgaacta gttgttcgag cggcgctgct
      541 gccacgagta cgccgcagcc ccggcgacca gcgtcatcgc accaagaagg aggatgatcg
      601 agccgttgtt gctgctggac gaaccctgac cggtgttcgg cagggcaacg acggccgcgg
      661 tcggcgcacc ggcagcggtg gtcggcgcga ctgtggtcgg cgtggtggtt gcgatcggag
      721 tcgcacccgg cgtggcttcc tgggcgcccg caacagcgga acccgtcacc agaaggctca
      781 aggaaaggag caaaatcact ccaagtcgac gaatgttcat tcctgtttct cccctcccct
      841 ggatgtccag gggtctccat ttttgctacg gtgattgcac ccacacgcgc tcactcgtag
      901 cgttccatgc ctgcgctcac gacaactcat tcgaccggga ttgcaagacc cgcatcaatg
      961 atgcggatcc ctcctgcact cagccgagtg gactcgcagc gcgaggcggc atcggacgcc
     1021 gcgtttccac tctaccacct gaggaattcg cccgcaatcc tcaaccggaa ggagcggacg
     1081 attcgccgcg ttttggcggt tctttttgtg tttttcccga tcatatccat tcgtgcaatc
     1141 cttgtgatgt ccagatttgg gcagatcgca cagcggccgc tgccgtattt gtcgcgtccc
     1201 ggattcggat tatgacggat ccgggacgga ttatgggcaa ttccgaggcc aataattgcc
     1261 taaggcgaat aatgaggccc tgattggggt aactgccgtc cattttcgcg cgggaatgtc
     1321 caaacggccc ggcgatggcc agtttgatgc gccggcgcaa gcgatcagaa ttcaggatag
     1381 tcgagagcgg gtgcgacgaa tccccgcacc ggatggacgc cgtcagcatt ggtcaccccc
     1441 ttcgagagac caagcacacg gaaccgtccc atgccccccg ggtcgatcag gcgcaggacc
     1501 gcagcctggg cctgatagta ctcgtcgatg gacgtctcac cgtcgtgctg gaacgtgacg
     1561 agcagatccc ccaggccgag tgcggcgaga aaatccgcct gcgtcgtcag cccgatctcg
     1621 tgcattccag ccccccttgc cgcagacgcg agccacgaga agtcgacgtg tgtggtcaga
     1681 tcctgctcgc cgatcgcagt gaaggggtcg tcggtcacgg tgtgctcgcg atagccacga
     1741 agcagcccgc cgagacgatg cccgcggtaa agctcggcgg cggggtagcc atagtcgacg
     1801 atcagggcgt atccacgctc gatcccctgc gcgacctcac ggatccagcc ggcggcgcgt
     1861 ggcgatacct cgaggatcgc gccatccgga agtgctcccg gtaccacccc ctgtgaaccg
     1921 agggcattca cgacgcggga gtccccgacc tcggcgctga gtgggccgag cgtggtcccg
     1981 aaccaggccg ccgcttcgtc ccacgtcacg aatcgttcgc ggaactcgtc gccatcgacg
     2041 accagatggt gagcggggag ggcatcgacc acctc
//
LOCUS       DASTMD010000174         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2873229, whole genome shotgun sequence.
ACCESSION   DASTMD010000174 DASTMD010000000
VERSION     DASTMD010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2873229"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFQ54_02605"
     CDS             complement(<1..404)
                     /locus_tag="VFQ54_02605"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU0163905.1"
                     /translation="MMIDDTNLLERTPIQVSHMPREIRIVAVSGAISNPSKTTALVRT
                     IAEHAVGCLGGESSVFDIAGLQPEIGQSLVAGELTPTLRCAVSAIEQADLLIAASPVF
                     NGGYSGLFKHLFDLVDPRALRGTPTILGATGGT"
     gene            complement(430..1767)
                     /locus_tag="VFQ54_02610"
     CDS             complement(430..1767)
                     /locus_tag="VFQ54_02610"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HEU0163906.1"
                     /translation="MSEQTRRHIQFGVFFQGVNFSTVWHWPESGSQTDFESFRRIAQT
                     AERGIFAAFFLGEGLRLREHLGKIHDLDVVGRHDPLTLVAALAGITDKIGLAVTQSTT
                     FNEPADLAYRLANLNLISGGRAAWNMVTTHNAWTGENFRRGGYLDAEDRYNHAESFVR
                     AVNAVWDAWEADSLATSTCAESWSKGDHTAVYKDDYYDIAVQRTLPAVPNGRVVTIQA
                     GDSSEGRDFAVRNANIIFSAHAELNDAATFAADIRQREAAAGRKDGDLKIFPGQIFIL
                     DDTEKAAQEKARWIHESQITPETAVKYLEQFWGRDLGEFAPDGPFPDVDPVVATTGAT
                     RGTGFQLAKAQEMATKWRTIAQENNFTLREFVIHWTSQGLGRGLVGTPLTIADEIQKY
                     VDNRAVDGFNITPFLVPSGLDDIVNDLIPELQNRDLYPTEYAGDTLREHLGIS"
     gene            complement(1760..2872)
                     /locus_tag="VFQ54_02615"
     CDS             complement(1760..2872)
                     /locus_tag="VFQ54_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0163907.1"
                     /translation="MATPLFLAIHLDGEGAHPAAWRAANHPPTALLTGKRIARIVTQA
                     ETAGFSIATFGGGHLPPGSAPNITARLDPVHQAAFAGPLTSRIGLVPEVQATFVEPFH
                     TANQLSSIDHSSRGRGGWIITADGSAAAARTYGAGAIDDPDALRREAHDVLEVSRLLW
                     DSWEDDAVIRDTTTWRYVDRDKLHYADFKGETFSVKGPSFVPRPPQGQLVVFGESDLV
                     DPREVEVTIVHAPDLAGLKAAAQDAHVNGATRVSADLEVILDSRGVPAAERLQRLNAH
                     AVWEQGANARLIGTADELIAAIGELADAVDIVRLIPAVIDTDLQELQFAVLRPLLRDG
                     IITAPLAGATLRETLGLEHPANRFMAVAQEERSRNV"
     gene            complement(2899..>3586)
                     /locus_tag="VFQ54_02620"
     CDS             complement(2899..>3586)
                     /locus_tag="VFQ54_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163908.1"
                     /translation="REDEEHSETLAIFWALLISVGIIAESTAHPSWSTISRTRDIPDW
                     WFGFFSYVDSGPPAERLELILALQRAGLVTFVGGDSRFEIDDDQGVFVAATGQHPETV
                     TARFMIDGFHPPRAVENSAEPVLRDIITTHQGYELVLGDREHPVPTGRLAVDEATAQV
                     LLPDGTPHPRRYAVGDFTSAPAAGTFARPGTNARIFRANDSIARSLLETIARASALHP
                     ALPMPESTRS"
BASE COUNT          565 a         1101 c         1296 g          624 t
ORIGIN      
        1 gtgccgcccg tggcgccgag aatcgtcggc gtcccccgga gtgcccgggg atcgacgaga
       61 tcgaagagat gcttgaacag gccgctgtag ccaccgttga agacgggtga cgccgcgatc
      121 agcagatctg cctgctcgat cgcggagacc gcgcaccgga gcgttggtgt cagctcgccg
      181 gcgacgaggc tctgaccgat ctccggctgg agtcccgcga tatcgaagac ggacgactcg
      241 ccgccaagac agccgacggc gtgctcggcg atggtgcgca cgagggcggt cgtcttcgat
      301 ggattgctga tcgcgccgga aacggcgacg atcctgatct ctcgcggcat gtgactcacc
      361 tggatcggcg tgcgctcaag aaggttcgtg tcatcgatca tcatgactgc tcgcggtgaa
      421 agcgacgggt caggagatcc ccagatgctc gcgcagcgtg tcgccagcgt attccgtggg
      481 atagagatcc cggttctgga gttccggaat caggtcgttc acgatgtcat cgagaccgga
      541 tggcacgagg aacggcgtga tgttgaagcc gtccaccgcc cggttgtcga cgtacttctg
      601 gatctcgtcg gcgatcgtca acggtgtgcc cacgagtccg cgcccgagcc cctgcgacgt
      661 ccagtggatc acgaactcac gcagcgtgaa gttgttctcc tgggcgatcg tccgccactt
      721 cgtcgccatc tcctgcgcct tcgcgagctg gaacccggtg ccgcgcgtcg cgccggtcgt
      781 ggcgacgact gggtcgacat cggggaacgg tccgtccggc gcgaactcgc cgagatcgcg
      841 tccccagaac tgctcgaggt acttgaccgc ggtctcgggc gtgatctggc tctcgtggat
      901 ccagcgcgcc ttctcctgcg ccgctttctc ggtgtcatcg aggatgaaga tctgccccgg
      961 gaagatcttg agatcgccgt ccttgcgccc ggccgcggct tcacgttggc ggatgtccgc
     1021 ggcgaaggtc gcggcatcgt tcagttcggc gtgggcagaa aagatgatgt tggcgttgcg
     1081 gaccgcgaag tcgcgtcctt ccgacgagtc accggcctgg atggtgacga cgcgaccgtt
     1141 cggcaccgct ggcagcgttc gctggacggc gatgtcgtag tagtcgtcct tgtagacggc
     1201 ggtgtgatcg cccttcgacc acgactcggc gcaggtcgag gtcgcgagac tgtcggcttc
     1261 ccaggcatcc cagactgcgt tcaccgcccg cacgaacgac tcggcgtggt tgtagcgatc
     1321 ctcggcgtcg aggtagccgc cgcgccggaa gttctcgccc gtccaggcgt tgtgggttgt
     1381 caccatgttc caggcggcgc ggccaccgga gatcaggttg aggttggcga ggcggtaggc
     1441 gagatcggcc ggctcgttga acgtcgtcga ctgcgtgacg gcaagcccga tcttgtcggt
     1501 gatcccggcg agggcggcga ccagcgtcag cgggtcgtgg cgaccgacga catcgagatc
     1561 gtggatcttg ccgaggtgct cgcgcaggcg gaggccctcg ccgaggaaga aggccgcgaa
     1621 gatgccgcgc tcggcggtct gcgcaatccg acggaaggac tcgaagtcgg tctgcgatcc
     1681 gctctccggc caatgccaga ccgtcgagaa gttgactccc tggaagaaga cgccgaactg
     1741 gatgtggcgc ctggtctgtt cagacattcc tgcttctttc ttcctgcgcc acagccataa
     1801 agcggttggc cggatgttcg agaccgaggg tttcgcgcag ggtcgcaccg gcaagtgggg
     1861 cggtgatgat cccgtcgcgc aggagtggac ggagtaccgc gaactggagc tcttgcaggt
     1921 cggtatcaat gacggcggga atcaggcgca cgatatcgac ggcatcggcg agctcgccaa
     1981 tcgcggcgat cagctcgtcc gcggtgccga tgagccgggc gttggctccc tgttcccaga
     2041 ccgcgtgcgc gttcagtcgt tgcaggcgct cggcggcggg gacgccgcgg ctgtcgagga
     2101 tgacctcgag atcggcgctg acccgcgtcg cgccgttcac atgagcgtcc tgggccgctg
     2161 ccttcagccc ggcgagatcc ggcgcgtgca cgatcgtcac ctcgacctcg cgcggatcga
     2221 cgagatcgct ctcgccgaag accacgagct gcccctgcgg cgggcgcggc acgaacgacg
     2281 ggcccttgac gctgaaggtc tcacccttga aatcggcgta atgcagcttg tcgcgatcga
     2341 cataacgcca ggtcgtcgtg tcgcggatga ccgcgtcgtc ttcccacgaa tcccagagca
     2401 ggcggctgac ttccagcacg tcgtgggcct cacggcggag ggcgtccgga tcgtcgatcg
     2461 ccccggcgcc ataggtgcgg gctgccgccg ccgagccatc ggcggtgatg atccagccgc
     2521 cgcgaccgcg cgagctgtga tcgatcgagg agagctggtt ggcggtgtgg aagggttcca
     2581 cgaaggtcgc ctggacctcg gggacgaggc cgatccgcga ggtcaacgga ccggcgaacg
     2641 ccgcctgatg gacggggtcg agccgggcgg tgatgttcgg ggcggaacct ggtggcaagt
     2701 gaccgccgcc gaaggtcgcg atgctgaagc ctgccgtctc ggcctgggtc acgatccgcg
     2761 cgatgcgctt gcccgtcagc agcgcggttg gcgggtgatt cgccgcacgc caggccgcgg
     2821 ggtgggctcc ttcgccatcg agatggatgg cgaggaagag gggcgtcgcc atggatgtcc
     2881 tccttggggg tgcgatcgtc acgatctcgt cgattcaggc atgggaagcg cggggtgcag
     2941 ggcgctggcc cgcgcgatgg tctcgagcag ggagcgcgcg atgctgtcgt tggcgcggaa
     3001 gatccgggcg ttggtgccgg gccgggcgaa ggtgccggcg gccggcgcgc tcgtgaagtc
     3061 gccgacggcg tagcggcggg gatgcggcgt gccatcgggg agaagcacct gggcggtcgc
     3121 ttcgtcgacc gcgaggcgac cagtgggaac gggatgttcg cggtcaccga gcacgagctc
     3181 gtaaccctgg tgcgtcgtga tgatgtcgcg gaggacgggc tcggcgctgt tctcgaccgc
     3241 gcgcggcggg tggaagccgt cgatcatgaa gcgggcggtg acggtctccg gatgctggcc
     3301 ggtggctgcg acgaagaccc cctggtcgtc gtcgatctcg aatcggctgt ctcccccgac
     3361 gaaggtgacg agaccggcgc gttgcaaggc gaggatcagt tccaggcgct cggctggcgg
     3421 tccactgtcg acatagctga agaaaccgaa ccaccagtcg gggatatcac gcgtgcggga
     3481 gatcgtcgac cacgacgggt gagcggtgct ctcggcgatg atgccgacgc tgatcaggag
     3541 cgcccagaag atcgcgagcg tctcgctgtg ctcctcgtcc tcgcga
//
LOCUS       DASTMD010000175         1913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2873254, whole genome shotgun sequence.
ACCESSION   DASTMD010000175 DASTMD010000000
VERSION     DASTMD010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2873254"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VFQ54_02625"
     CDS             complement(<1..1283)
                     /locus_tag="VFQ54_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEU0163909.1"
                     /translation="MATMMQVPPRGEDEGIGLSPDQIAILKEIEQRVLWLATLIVHHA
                     NNVRPNPDKPTKVGGHQASSASVATILTALYFQFLKGGDRVSVKPHASPVFHACQYLL
                     GNLDRKYLTMLREMGGLQSYPSQTKDPDPVDFSTGSVGVGAVAPLFASMAAKYAEMRF
                     GSVTSERFITLLGDAELDEGNIWEAVIDDTLTQLNNVIWIVDLNRQSLDRVIPGIKAA
                     KLKRLFASAGWEVFEAKYGTSLEAVMQAPGGDAIRNRIDTMPNEEYQALIRIADGAEV
                     RRRMADVADRAAHDAILAALVDVSDEQLPAIVANLGGHDLPKLLQVLQAADAVPDRPV
                     VIFAYTVKGYGLPMAGDPMNHSQLLTNAQIDQVRELIGIPEDQIWDPFPADSPAGEWC
                     AEAGERLKKVSLGIELDPSLVPDDVATRHPNATST"
     gene            complement(1544..>1913)
                     /locus_tag="VFQ54_02630"
     CDS             complement(1544..>1913)
                     /locus_tag="VFQ54_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163910.1"
                     /translation="LAIAFAFAPWIQYQTRSGAVATHNAVGSDGVFLVIVATIAVVSL
                     VFVGFMDDPAVPATAAFAATFVGLLIAGSNWIWVDSIHPVDASIWQAPTTGWGVVAAT
                     FACLLGSIYTWSVMRIARVF"
BASE COUNT          341 a          572 c          671 g          329 t
ORIGIN      
        1 tgggtggatg tggcgttcgg atgacgggtc gcgacatcgt ccggcacgag gctcgggtcc
       61 agctcgatcc cgagcgagac tttcttgagg cgctcgccag cctcggcgca ccactcaccg
      121 gcgggtgaat ccgcggggaa cggatcccag atctgatcct ccgggatgcc gatcaactcg
      181 cgcacctgat cgatctgggc attggtcagg agctgggaat ggttcatcgg gtcgccggcc
      241 atcggcaggc cgtacccctt caccgtgtag gcgaagatga cgaccgggcg atcgggcacg
      301 gcgtcggccg cctggaggac ctggaggagc ttcggcaggt cgtgaccgcc gagattggcg
      361 acgatcgccg ggagctgctc gtcggagacg tcgacgaggg ccgcgagaat cgcatcgtgg
      421 gcggcacgat cggcgacatc ggccatgcga cgccgcacct cggcgccgtc ggcgatccgg
      481 atcagcgcct ggtactcctc gttcggcatc gtgtcgatcc ggttgcggat cgcgtcgcca
      541 ccgggcgcct gcatcacggc ttcgagcgag gtgccgtact tcgcctcgaa gacttcccag
      601 ccggcgcttg cgaagaggcg cttgagcttg gctgccttga tgcccgggat gacgcggtcg
      661 aggctctggc gattgagatc gacaatccag atcacgttgt tcaactgggt gagcgtatcg
      721 tcgatgacgg cctcccagat gttcccctca tcgagttcgg cgtcaccgag gagcgtgatg
      781 aagcgttcgc tggtgaccga tccgaagcgc atctcggcgt acttggccgc catcgaggcg
      841 aagagcggcg cgacggcgcc gacgccgacc gatccggtcg agaagtcgac ggggtccgga
      901 tccttcgtct gcgacgggta ggactggagt ccgcccatct cgcggagcat cgtcaggtac
      961 ttgcggtcga ggttgcctag caggtactgg caggcgtgga agacgggcga ggcatgcggc
     1021 ttcacggaga cgcgatcgcc gcccttgagg aactggaaat agagcgcggt caggatcgtc
     1081 gcgaccgagg ccgacgaggc ctggtggcca ccgaccttgg tcggcttgtc cggattcggg
     1141 cggacattgt tcgcgtgatg aacgatcagc gtggcgagcc agaggacgcg ctgctcgatc
     1201 tccttcagga tcgcgatctg atcgggagag agtccgatcc cttcatcctc gccgcgtggc
     1261 ggcacctgca tcatggttgc catcgaagtg gttccttgtt ggtagtcgtc atcgcacgtg
     1321 tcgcgcgagg ttgccgcatc gggagacccg gggaacgaag gtcatccgca ttctgtcgaa
     1381 atctggggaa tgacaacatc cgtggaggtg atggtacaac gcgcggcatc gaatggaaat
     1441 gcccgtgggc cagagggcgt tgtggtctga cgtcacatct tttggcgaaa cggcgggcca
     1501 atcgccggca ttcgcgagct cggcgccgag tggttctggc gcgctagaag actcgcgcga
     1561 tccgcatcac actccaggtg tagatcgaac cgaggagaca ggcgaaggtc gccgcaacta
     1621 cgccccagcc ggtcgttggc gcctgccaga tgctggcatc cacggggtgg atgctgtcga
     1681 cccagatcca gttagaaccg gcaatcagca aaccgacgaa ggtcgccgcg aacgcggccg
     1741 tcgccgggac cgccggatca tccatgaacc cgacgaagac gagcgagacg acggcgatgg
     1801 tggcgacgat cacgaggaag accccgtcgg aaccgaccgc gttgtgcgtc gccacggcgc
     1861 cggagcgcgt ctggtactgg atccagggtg cgaaggcgaa ggcgatcgcc agg
//
LOCUS       DASTMD010000176         4219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2337362, whole genome shotgun sequence.
ACCESSION   DASTMD010000176 DASTMD010000000
VERSION     DASTMD010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2337362"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..666
                     /locus_tag="VFQ54_02635"
     CDS             55..666
                     /locus_tag="VFQ54_02635"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEU0163911.1"
                     /translation="MKTTRLEAFSDGVFAIAITLLIIEVKVPEVESGALWHALRDQWS
                     SYAGFVVSFLVIGVIWVNHHTMFDTIVKADEKLLFLNLILLMVVAFIPFPTALLAHYL
                     EAGHDAHVAAYVYSATMLAMGLAYTAVWAYAMRTKGIVTDRLDSRARRVLLNRSLLGC
                     VIYAVSFGAAAIGAELALAFHAVVALLFVLSPRPQVAEEGLAG"
     gene            complement(736..1071)
                     /locus_tag="VFQ54_02640"
     CDS             complement(736..1071)
                     /locus_tag="VFQ54_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163912.1"
                     /translation="MTASFDTTIETITRSLTIPTTIGATAAIQRLRAADEAMRASSSW
                     LKPEAVTVTWESTSAAIAGTAFGFPVTGEVDATDAAVTITLHIPWTAKALLASFGPRL
                     EAQLGQLLA"
     gene            complement(1085..1387)
                     /locus_tag="VFQ54_02645"
     CDS             complement(1085..1387)
                     /locus_tag="VFQ54_02645"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEU0163913.1"
                     /translation="MPSSVVKVTHTLGQAEAVDRLKNILASAKEQYGDRISDLQENWN
                     DKGGTFSFKAMGFKISGELAVTDTDATITGEFPWAAKPFQKTIETTLRERAERLLA"
     gene            complement(1495..2526)
                     /gene="thiS"
                     /locus_tag="VFQ54_02650"
     CDS             complement(1495..2526)
                     /gene="thiS"
                     /locus_tag="VFQ54_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEU0163914.1"
                     /translation="MNLIVNQQPLIVEREAATVADFLRDRNLPEKAIVVAINGDIVAK
                     TDWPTTVLKEGDDVEIVRAVSGGDHVTFEPDPLVIAGRAFSSRLFLGTGKYPDTRSMT
                     DALAISGTEMVTVAIRYMNLDALNGDATSLLDELDLTKYHLLPNTAGAYTTADAVKMA
                     RLARAATGTNWIKLEVIGDQQTLWPDVAATIEATKLLVAEGFVVLPYTSPNLVAALRL
                     EEAGAATVMPLASPIGSGQGMQDWTSIQRIVERISVPVVVDAGIGVPSDAALAMEIGA
                     DAVLVNTAVARANDPVRMALAMKLGVEAGRHSHLAGRIARTTHANASSPTTGVPLATA
                     AAGAGGVIE"
     gene            complement(2523..3170)
                     /gene="thiE"
                     /locus_tag="VFQ54_02655"
     CDS             complement(2523..3170)
                     /gene="thiE"
                     /locus_tag="VFQ54_02655"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU0163915.1"
                     /translation="MALFERPHLQYITEVTPLLGSEGFDRIVAAVEGGVDSIQLRERG
                     VSARTMFDIASRLIPIAATHDVAIAINDRIDVAAALDGVGLHLGGHSLPIATAREIAG
                     PRIIGVSTHSLDDARIQAASGADYVTFGHIFATTSHPGEIPAGLATLREVAAVVNVPI
                     LAIGGVDRGNIAGVLAAGAAGITVISAIRNAFDPYVATRELRHALDHLPAPGAIA"
     regulatory      complement(3269..3372)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3454..4032
                     /locus_tag="VFQ54_02660"
     CDS             3454..4032
                     /locus_tag="VFQ54_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEU0163916.1"
                     /translation="MKGFGFRLIVATMLFSVTMMDGGGADAAKRVTPTPDTFTVDGAE
                     PHKLPKDAEKMIVHKVVDGDTVQLTYPNDDYYYVTRIIGINAPESVKPNSPVECFGPQ
                     ASDRLKELLPIGSVVYVSRDVSDEDQYGRRLRNVWIRDADSGEAFLVSEILVRGGYAD
                     AREYRPDDEYDDELAAAERDAKQDDAGMWGTC"
BASE COUNT          708 a         1287 c         1445 g          779 t
ORIGIN      
        1 ggatgatttc ccatgtccgg ggcggatgga gattcggcaa caggggatgg gaccatgaag
       61 accacgagac tcgaggcctt ttccgatggc gtcttcgcga tcgcgatcac gctcctgatc
      121 atcgaagtga aggtgccgga agtcgagtcc ggcgcactct ggcacgcctt gcgggaccag
      181 tggtcatcgt atgcgggctt cgtcgtcagc ttcctcgtca tcggcgtgat ctgggtcaat
      241 catcacacga tgttcgacac catcgtcaaa gcggacgaga agctgctctt cctcaacctc
      301 atcctcctga tggtcgtcgc gttcatcccg ttcccgacgg cgctgctcgc gcactatctc
      361 gaggcggggc atgacgccca cgtcgcggcg tacgtctaca gcgcgacgat gctggcgatg
      421 gggctggcct acacggcggt ctgggcgtat gcgatgcgga cgaagggcat cgtcaccgac
      481 cggctggata gccgggcacg gcgcgtcctg ctcaatcgct cgctgctcgg ctgtgtcatc
      541 tatgcggtgt cgttcggcgc ggcggcgatc ggcgccgaac tcgcgctcgc cttccacgcc
      601 gtcgtcgcgt tgctcttcgt cctcagcccg cgtccccagg tggcggaaga ggggctggct
      661 ggatgaaacg cggaagcgcc gtgtggatga gattccacac ggcgcctcct gcattcctgt
      721 cgcgatccga acgaatcagg cgagcagttg tccgagttgc gcctcgaggc gcggtccgaa
      781 gctggccagc agcgccttcg ccgtccacgg aatgtggagc gtgatcgtca ccgcggcatc
      841 ggttgcatcg acctcaccgg ttacggggaa gccgaaggct gttcccgcga tcgcggccga
      901 ggtcgactcc caggtgacgg tcacggcctc cggcttcagc caggaggacg aggcgcgcat
      961 cgcctcgtcg gcggcacgga gccgctggat cgcggcggtg gcgccgatcg tggtcgggat
     1021 cgtcagcgat cgggtaatgg tctcgatcgt cgtatcgaag gacgcggtca ccgggcggac
     1081 cttcctacgc gagcaggcgc tcggcgcgct cgcggagcgt cgtctcgatc gtcttctgga
     1141 acggcttcgc agcccacggg aactcgcccg tgatcgtcgc atcggtatcg gtcaccgcga
     1201 gttcgcccga gatcttgaaa cccattgcct tgaaggagaa cgtcccgccc ttgtcgttcc
     1261 agttttcctg gagatcggaa atgcggtcgc catactgctc cttggcgctg gcaaggatgt
     1321 tcttcaggcg gtcgacggct tccgcctgac cgagcgtgtg ggtaaccttg acgactgagc
     1381 taggcatgaa cggctgactc ctctatgggc gtgatggtgg cgaagaaaac cggcgtggat
     1441 tcgagatgca tgtgcttccg cgccggatga cgccctcaag gatagtcgat gggtctattc
     1501 gatgacgccg ccggctcctg ccgccgcggt ggcaagcgga acaccggtcg tcgggctcga
     1561 ggcgttcgcg tgggtggtcc gcgcgatccg tcccgcgagg tggctgtgac ggcccgcctc
     1621 cacgccgagc ttcatcgcga gcgccatccg caccggatcg ttcgcgcggg cgaccgccgt
     1681 attgacgagc acggcgtccg cgccgatctc catcgcgagc gcggcatccg aaggtacccc
     1741 gatcccggca tcgacaacga cgggaaccga gatgcgctcg acgatgcgct ggatgctggt
     1801 ccagtcctgc atcccctgcc cggatccgat cggcgaggca agcggcatca cggtcgccgc
     1861 gcccgcctct tcgagccgta gcgcggcgac gagattggga cttgtatatg gaagaacgac
     1921 aaagccctcg gcgacgagga gcttcgtcgc ctcgatcgtg gcggcgacat cgggccagag
     1981 ggtctgctgg tcgccgatga cctcgagctt gatccagttc gttcccgtcg cggcgcgcgc
     2041 gagtcgtgcc atcttcaccg cgtcggcggt cgtataggcg ccggcggtat tcggcaggag
     2101 gtggtacttc gtgagatcga gctcatcgag cagcgacgtc gcgtcgccgt tcagggcgtc
     2161 gagattcatg tagcggatcg cgaccgtcac catctcggtg ccggagatcg cgagcgcatc
     2221 cgtcatcgac cgcgtgtccg ggtacttgcc ggtgccgagg aagaggcggg aggagaacgc
     2281 tcgtccggca atgaccagcg gatcgggctc gaaggtcacg tgatcgcctc cggataccgc
     2341 gcgcacgatt tccacgtcgt ctccctcctt gaggaccgtt gtcggccagt cggttttcgc
     2401 cacgatgtcg ccgttgatcg cgaccacgat cgccttctcg gggagattgc gatcgcgcag
     2461 gaagtccgcg actgtcgcgg cctcgcgttc gacgatgagt ggttgctgat tgacgatcag
     2521 gttcatgcga tggctcctgg tgccggaaga tggtcgagtg cgtgccggag ttctcgcgtc
     2581 gcgacgtagg gatcgaatgc gttgcggatc gcggagatga cggtgattcc ggcggctccg
     2641 gccgcgagga caccggcgat gttcccgcga tcgaccccgc caatcgcgag gatcggcacg
     2701 ttcaccacgg cggcgacttc cctgagcgtg gcgaggccgg cgggaatctc accgggatgc
     2761 gaggtcgttg cgaagatgtg gccgaacgtg acgtagtccg ccccgctcgc cgcctggatt
     2821 cgggcgtcat caagcgaatg cgtggagacg ccgatgatcc gcggcccggc gatctcgcgg
     2881 gcagtcgcga taggcagcga gtgaccgccg agatgcagcc cgacgccgtc gagcgcggcg
     2941 gcgacgtcga tgcggtcgtt gatcgcgatg gcgacgtcgt gggtagccgc gatcgggatg
     3001 agacggctcg cgatatcgaa catcgtccgg gccgaaacgc cacgctctcg cagttgaatc
     3061 gagtcgacgc cgccttcgac cgcagcgacg atgcgatcga atccctcgct gccgagcagc
     3121 ggcgtcactt cggtgatgta ttgcaggtgg gggcgctcga atagtgccat ggcgacctcg
     3181 catgattgcg ggcgccgtct gaaaacagcg acgccgcgct cgaaatcgat cgatggcgcg
     3241 gcggagaagc gatgggtaaa ccatggtcgc ttgctcttcc gcgctttcct ccgccggtcc
     3301 taaccggttc aggttcaaag ggactctctc agccgggtct cgtcgagacc gtcagcaccc
     3361 ctagcgcgtt tcgaggatgg tagcacggcg gcaatcggtt ggcccatggt atgttgggga
     3421 aactgatgtc gatcaagatg gggtggggtt ttgatgaagg gattcggatt ccggctgatc
     3481 gtggcgacca tgcttttctc ggtcacgatg atggatggcg gcggtgccga cgccgcgaag
     3541 cgcgtcacgc cgacgcccga cacgttcacc gttgacgggg cggagccaca caagttgccg
     3601 aaggatgccg agaagatgat cgtgcacaag gtcgtcgatg gcgatacggt gcaactcacc
     3661 tatccgaacg atgactacta ctacgtgacg cggatcatcg ggatcaacgc gccggaatcg
     3721 gtgaagccca attccccggt cgaatgcttc ggcccgcagg cgagcgatcg cctgaaggag
     3781 ctgcttccca tcggatcggt cgtctacgtc tcgcgggatg tctccgacga ggatcagtac
     3841 gggcgaaggc tgcgcaatgt ctggatcagg gacgcggatt cgggcgaggc gttcctggtc
     3901 tccgagatcc tggttcgcgg cggctacgcc gatgctcgcg aataccgacc ggacgacgag
     3961 tacgacgacg aactcgcggc tgccgagcgc gatgcgaagc aggatgacgc cgggatgtgg
     4021 ggcacctgct aggcgacagc aaggtggaac cccgcttcgc ggtgcattct tagccggttg
     4081 gggccaccgt caatgacacg gggggtgacg actacagcga aatccagaac ggttgccgaa
     4141 ggtgctactg gaacggatcc ggtattccgc ggtgcgcccg gtcgttcgta gggcgggact
     4201 gccgctgccg gaccggagg
//
LOCUS       DASTMD010000177         3331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1444954, whole genome shotgun sequence.
ACCESSION   DASTMD010000177 DASTMD010000000
VERSION     DASTMD010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444954"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFQ54_02665"
     CDS             complement(<1..699)
                     /locus_tag="VFQ54_02665"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU0163917.1"
                     /translation="MLEPKVAKDHQYYQDLVGGAAIGILTPEEYAELEAYMRTSDGES
                     LREELAEFRAVVDAIPLSLDAQAPSPELRDRIEAMIAPEAIDRELPSATTPPALFSLP
                     TSPLAPPETLSLPTPLAPRRQATGVYLAVAAALIIAIVAGALVGRYLLSDNDTTDKGQ
                     EFALSFANPAPDESAKLTYYPDRQLFVLNSVDLPALPDNKVYQVWLIGASGAKPVGTV
                     NGSEYATVADIDQFQ"
     gene            complement(748..1329)
                     /locus_tag="VFQ54_02670"
     CDS             complement(748..1329)
                     /locus_tag="VFQ54_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0163918.1"
                     /translation="MTPQPPDDTDLIQGIAERESQALMQLYDRYNRLCFAVAYRIVDD
                     ASLAEEVVQDAFLQVWNRASTFDLERGRNVRAWLLTIVHHRSIDFRRRNHDRQVPQVS
                     LDDVDNLLSAPDVWKDVSANLTREQMRAAIDTLPPDQRRTIELAYFEGLSHGEIAERE
                     STPLGTIKGRLRLGLKKLHAELLPMMEQANAPP"
     gene            1551..2105
                     /locus_tag="VFQ54_02675"
     CDS             1551..2105
                     /locus_tag="VFQ54_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0163919.1"
                     /translation="MTPPSAIPILTGERIVLRPFVAADAPIVQRLLQRPEISATTLNV
                     PYPYPEGTALAWIAGHPEQAALGRGFAWAICRKDDGELMGTIGLGVTPRHQRGTLGYW
                     LGVDYWNQGFTTEAARLVTTFGFEKIGLHRIDAACLPENIGSSRVMEKSGLRYEGTQR
                     GYYRKGDRFLDVAWYAAIATDPRS"
     gene            complement(2378..>3331)
                     /locus_tag="VFQ54_02680"
     CDS             complement(2378..>3331)
                     /locus_tag="VFQ54_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEU0163920.1"
                     /translation="DRDWPSDYDLDNARIHARATRRHVLKIGAAAGVTAFGIGAVARG
                     AVAQATATSATPDATAVAATPATSMCVLTPELTEGPYYLDDLLVRNDITEGKAGIPLD
                     LKITVLDSSTCTPLANAAVDVWHCDANGFYSGYTDKNPGGGDAYVDDGSDPDTFLRGV
                     MLTDDNGLAEFQTIYPGWYTGRDIHIHMKVHVGGATEDGTYDGGTVAHTGQLAFEDSL
                     TDEVAKIEPYASRTTTFLRFEDDGVFGGVEDGDTSYFLTMPPKNADSLADGFTGTINV
                     GVDPTANHTEDAGMGGGGGNPGGDPGSGNPPGGNPPDGNGN"
BASE COUNT          575 a         1075 c         1074 g          607 t
ORIGIN      
        1 ctggaactgg tcgatgtcgg caaccgttgc atactcgcta ccgttcacgg ttccgacggg
       61 tttcgcgccg ctcgccccga tcaaccagac ctgatagacc ttgttgtccg gcagcgccgg
      121 caggtcgacc gaattcagga cgaagagttg ccgatccggg tagtaggtca gcttcgccga
      181 ttcgtccggg gccggattcg cgaaactgag tgcgaactcc tgccccttgt ccgtggtgtc
      241 gttgtcagaa agcaggtagc gaccgacgag tgccccggcg acgatcgcga tgatgagtgc
      301 ggccgccacc gcgaggtaga cgccggtcgc ctgccgccgc ggcgcgagcg gggtcggcaa
      361 cgataacgtc tcgggcgggg ccagcggaga cgtgggcagg gagaagagcg ccggtggtgt
      421 tgtggcggac gggagttcgc gatcgatcgc ctccggggcg atcatggcct cgatccggtc
      481 gcgaagctcc ggcgatggcg cctgcgcgtc gagcgaaagc gggatcgcgt cgacgaccgc
      541 ccggaactcg gccagttcct cgcgcaggga ttccccatcg ctcgtgcgca tgtaggcctc
      601 gagctcggca tattcctccg gcgtgaggat gccgatcgcg gcgcctccca cgagatcctg
      661 ataatactga tggtcctttg cgaccttcgg ttccaacatg tccaccttga tatgccgtca
      721 gctcacgcgc gtcccgggcg atccggatca cggcggggcg ttcgcctgct ccatcatcgg
      781 aagcaattcc gcgtggagct tcttcaatcc gagtcgaagc cgtcccttga tcgtgcccag
      841 cggggtcgat tcacgctccg cgatctcacc atgggagagc ccttcgaaat aggccagttc
      901 gatcgtgcgt cgttgatcgg gaggaagcgt atcgatcgcc gcccgcatct gctccctcgt
      961 cagattcgcc gagacatcct tccagacatc cggcgccgag agcaggttgt cgacatcgtc
     1021 gagcgagacc tggggaacct gacgatcgtg attgcggcga cgaaagtcga tcgagcgatg
     1081 atggacgatc gtcagcagcc aggcgcgcac gttacggccg cgttcgagat cgaaggtcga
     1141 cgcgcggttc cagacctgga gaaaggcatc ctgcacgact tcttccgcga gcgacgcgtc
     1201 gtcgacgatc ctgtaggcaa cagcaaagca gagccggtta taccggtcgt aaagctgcat
     1261 cagcgcctgc gattctcgct cggcgatgcc ttggatgaga tcggtatcgt cgggcggctg
     1321 cggcgtcacg cggtggaaga cctcccattg ctctcttcaa cgctgcgctt ggcggaatgg
     1381 atcatttcct tccccgaacc tcgccaaaag gagagggaag gacggatggc ggcaagccgg
     1441 gctcgggtac aatcagccga tgtcgcgtcc cggggatcga tcgctggctg atgcgtccaa
     1501 tcatacctga ccggaaacac cctgaattca tccttcctct tcgagaaccc atgactccac
     1561 caagcgccat ccccatcctc acgggcgagc gtatcgtgct ccgccccttc gtggccgccg
     1621 acgctccgat cgtccagcgc ctgcttcagc gaccggagat ttccgcgacg acgctgaatg
     1681 tcccctaccc gtacccggaa ggaacggcgc tggcatggat cgccggacat ccggagcagg
     1741 cggcgctcgg tcgcggcttc gcctgggcga tctgccggaa agatgacggc gagttgatgg
     1801 ggacgatcgg actcggcgtg acgccaaggc atcagcgcgg caccctcggc tactggctcg
     1861 gcgtcgacta ctggaaccag ggattcacga cggaagctgc ccgcctcgtc accaccttcg
     1921 gcttcgagaa gatcggcctg caccggatcg acgccgcctg tctccccgag aacatcggct
     1981 catcccgcgt gatggagaag tccggtctgc gctacgaggg aacgcagcgc ggctactacc
     2041 ggaagggcga ccgcttcctc gatgtcgcct ggtatgccgc catcgccacg gaccctcgct
     2101 cgtagggaca gtcgtcccgc gtaacgggcg gggatgccac gccattctgc gagtgggtgt
     2161 ccgaatccgt gacgccatgg cacgcgctct gcattacgtc atgtgagggc taccccgccc
     2221 tccccctcat gccctggctc gcctcccccc aaactccagc caaatcgagc cagggctccc
     2281 ctgaaaccag atggtgaacg cccgggatcg acactgatcc cgggcattca tttcgcgaac
     2341 atggatgttg cgctggttgg agcggctcag caggatccta gttcccgttg ccgtccggag
     2401 gattgccgcc cggcggattg ccgctgcccg ggtcaccgcc ggggttgcca ccgccgccac
     2461 ccattccggc atcctcggtg tgattggccg tcggatcgac gccgacattg atcgttcccg
     2521 tgaacccatc ggcaagggag tcggcattct tcggcggcat cgtcaggaag tagctcgtgt
     2581 ccccatcctc gacaccgccg aagacgccgt cgtcctcgaa gcggaggaac gtggtcgtcc
     2641 tgctggcgta gggctcgatc ttcgcgacct catcggtgag ggaatcctcg aaggcgagct
     2701 ggcccgtatg cgcgacggtg ccgccgtcgt aggtgccatc ctcggtcgcg ccgcccacgt
     2761 gcaccttcat gtggatgtga atgtcgcgcc ccgtgtacca gccgggatag atcgtctgga
     2821 actcggcaag cccgttgtca tcggtcagca tgacgccgcg aaggaaggtg tcgggatcgg
     2881 atccgtcgtc gacgtaggca tcgccaccgc cgggattctt gtccgtgtag ccggaataga
     2941 agccgttcgc atcgcaatgc cagacgtcga cggcggcatt ggcgagcggg gtgcaggtgg
     3001 aggaatcgag gacggtgatc ttgagatcga gcggaatccc cgccttgccc tcggtgatgt
     3061 cgttccggac gaggagatcg tcgagataat acggtccctc cgtaagttcc ggcgtcagaa
     3121 cgcacatcga ggtggctggg gtggcagcga ccgccgtcgc atctggcgtc gccgatgtcg
     3181 cggttgcctg ggcgaccgcg ccgcgcgcaa cggcgccgat cccgaaggcg gtcaccccgg
     3241 ccgccgcgcc gatcttgagg acatggcggc gcgttgcccg cgcgtgaatt cgcgcattgt
     3301 ccagatcgta atcggagggc cagtcgcggt c
//
LOCUS       DASTMD010000178         1976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_642007, whole genome shotgun sequence.
ACCESSION   DASTMD010000178 DASTMD010000000
VERSION     DASTMD010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642007"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFQ54_02685"
     CDS             complement(<1..725)
                     /locus_tag="VFQ54_02685"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU0163921.1"
                     /translation="MDESNDAMALATVIAVAAGYHSRVAHARFLTGGISARIVAFDLV
                     DGTGPLVTRLYGVNDVVSNPDIAHLEFDVLHHVSACGIPVARPIMVIDPGVLGPRPGL
                     VTTLIDGDPGQRPADDMAVAGILANALRAIHGVHVSPKLRSQLPVAMFQPERWSTRWR
                     EIGISPQLMERLTLFSRQGHATRDVLLHGDFWPGNVLWANGSLSGIVDWEDVHFGDYR
                     FDVAGGFLELVLATTLEAGQAFLA"
     gene            1222..>1976
                     /locus_tag="VFQ54_02690"
     CDS             1222..>1976
                     /locus_tag="VFQ54_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163922.1"
                     /translation="MNQPSWNHQQTSPMPFHRYVPYEQRVHIPAFERTWPTRRLTHAP
                     LWVPVDLRDGNQALAEPMGPDRKRTMLDLLLRVGFKEIEIGYPSASQADFDFVRMLVE
                     KDLIPDDVTVVAFTPARPDLIARTFEAIDGMANVVVHMYTATAPIWRERVLRHSRESL
                     LDLIVAGGEAVLRGAGEREGIRFQFSPEVFNQTEPDYILEVCKRMTDLWEASPERPVI
                     LNLPAVEIATPNVYADQIEYMHRNLDRRESVILS"
BASE COUNT          417 a          650 c          547 g          362 t
ORIGIN      
        1 gccaggaacg cttgacccgc ctccagggtt gtggcaagaa ccagctccag aaagcctccc
       61 gccacatcaa accggtaatc accgaaatgg acgtcctccc aatcgacgat tccggagagc
      121 gatccgttgg cccaaaggac attccccggc cagaaatctc cgtggaggag aacgtctctc
      181 gttgcgtgac cctggcgcga gaaaagtgtg agtcgctcca taagttgtgg cgagattcca
      241 atctcccgcc atctggtgct ccagcgctca ggttggaaca tcgcaacagg cagctggctt
      301 cgcagtttcg gggatacgtg tacgccatga atagctcgca acgcgttcgc caatattccg
      361 gccactgcca tatcgtcggc aggacgctga cctggatccc cgtcgatcag cgtcgtaacc
      421 agccccggac gagggccgag aactcccgga tcgatcacca tgatcggcct cgccacggga
      481 atgccacatg cggaaacatg gtgaagtacg tcaaactcca gatgtgcgat gtcgggattt
      541 gatacgacat cattgactcc atagagtctc gtcaccaatg gcccggtacc atccacaagg
      601 tcgaaggcaa caatccgtgc agaaattccc ccggtgagaa atcgggcatg tgcaactcgg
      661 gagtgatatc cggcggcaac ggcgataaca gtcgccagcg ccatcgcgtc attggattcg
      721 tccacgaaat gacctcctgg ctggcagtgc ctgaccagtc cctgatccac atcggtccat
      781 cagatgacaa ccctgtcgaa ctcgaaaatc ccacaagcat tcggtgagca ccgtcgatcg
      841 agcacgctag gtcgaggtgc tctaccgtat ccacgtgcca caacatggcc gaccactcgc
      901 atacttgcgc attcgttgag tccgaaccga cagaccttcc cagagagacc tccgtgtcct
      961 gccgcaggaa gtccagcctt ggggataacg cgatcgaggc cgcgacttgg tggatacacc
     1021 acgtaccaga tgcgatcgcg aaggaggtcg gagacctgtt ggaccagcgg gtagcgtcga
     1081 atcgtcatcg tgcggtatcg tgctcctgac tcctcagaca agctgatgag ttcctgtcgt
     1141 aatccgccgt tccgcgaacg ccgggccgct cgcgccgagg attgccatgc gtggcggcat
     1201 cgtacatcga aggatccttc catgaatcag ccgtcctgga atcaccagca gacctccccg
     1261 atgccgttcc atcgctacgt cccgtacgag caacgcgttc acatccccgc attcgagcgg
     1321 acgtggccaa cacggcgact gacacatgca ccgctctggg tacccgtcga tctccgcgac
     1381 ggcaaccagg cgcttgctga accgatgggt ccggatcgca agcgcacgat gctcgatctc
     1441 ctcctccgcg tcggcttcaa ggaaatcgag atcggctacc cctcggcgag ccaggccgac
     1501 ttcgacttcg tccgcatgct cgtcgagaag gacctgatcc ccgatgacgt caccgtcgtc
     1561 gccttcaccc ccgcccggcc cgatctgatc gcccggacgt tcgaggcgat cgatggaatg
     1621 gcgaacgttg tcgtccacat gtataccgcc accgcgccga tctggcgcga gcgcgtcctc
     1681 cgtcattcac gcgaatcgct gctcgacctg atcgtcgccg gcggtgaggc cgtgctccgc
     1741 ggcgcgggcg agcgcgaggg catccgcttc cagttctcgc cggaagtctt caaccagacc
     1801 gaaccagatt acatcctcga ggtctgcaag cggatgaccg atctctggga ggcatcgccg
     1861 gagcggccgg tcatcctcaa tctcccggcg gtcgagatcg caacaccgaa tgtctacgcc
     1921 gaccagatcg agtacatgca tcgcaacctc gaccgccggg agagcgtgat cctctc
//
LOCUS       DASTMD010000179         1938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_195792, whole genome shotgun sequence.
ACCESSION   DASTMD010000179 DASTMD010000000
VERSION     DASTMD010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195792"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1027)
                     /locus_tag="VFQ54_02695"
     CDS             complement(143..1027)
                     /locus_tag="VFQ54_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0163923.1"
                     /translation="MSQVALTGRATTTGRRNTTTSSGASWGSPVTYLVAMVLISICIA
                     PVLYIIIGGFRSNSQITVSPAGWPDPWVLTNYTSVLQSGLFWQQFGNSMISAITTTVV
                     AVALALMASFVIARYSFAGRNAMYALFAAGLMFPMTVAITPLYLLVKNLGLTNNLLGV
                     ILPQIAFSLPTTVIILVPFLAAIPKELEEAASIDGASRLGFFFRMVLPLSLPGVITTG
                     ILVFIQTWNQYLLPLFILNDSKSYTLPLGVQSFSSEHSVDTAAVLAFTSLSMIPALVF
                     FSIFERRIVGGLTGAVKG"
     gene            complement(1027..>1938)
                     /locus_tag="VFQ54_02700"
     CDS             complement(1027..>1938)
                     /locus_tag="VFQ54_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0163924.1"
                     /translation="DWRKRFEIAVLSGPAIVMFLAFVIFPVFMAAYYGFFRWKGFGVP
                     TNFVGFGNYVTIFNDQNFRDALTHNGFILVMSLVMQGPAAVAFALLLNQNIRGRSLIR
                     VLIFVPYVISEVIVGTGWGLMLQYTGALNDLLTNMGLGAFAHDWIADPNIAIWTLMVI
                     ISWKYIGFAVILMLAGIQGIPEELYEAAAIDGASYWQIQRWITLPLLGPTIRVWAFLS
                     IIGSLQLFDLVYIIWGQYVASTAGTSTMATYMVREGRLAGNYGFGNAVAVVIFVISLA
                     IALTYQRFVLRRDTAGALTEAPGKKQN"
BASE COUNT          412 a          544 c          676 g          306 t
ORIGIN      
        1 tcaagcgtca tgcgcgagag caactcgctg acgcgagggg agacctcggg gatcgcgggg
       61 atcgaggcgg acgacgtgga cacagggccc agctccttca tgggttggtg atcgggattg
      121 acgcaggcaa ggatcctgcg cgtcagccct tgacagcgcc ggtgagccca ccaacgattc
      181 ggcgttcgaa gatgctgaag aacaccagcg cggggatcat ggacagtgaa gtgaacgcga
      241 ggacggcagc cgtgtccacc gaatgctcgg acgagaacga ctggacaccc agcggcaggg
      301 tgtagctctt ggagtcgttc aggatgaaca ggggcagcag gtactggttc caggtctgga
      361 tgaagaccag gatgccggtg gtgatgacgc ccggcaggga caacggcagc accatgcgga
      421 agaagaagcc cagccggctg gcgccgtcga tggaggcggc ctcctccagt tccttcggga
      481 tggctgcgag gaagggcaca aggatgatca ccgttgtcgg cagcgagaag gcgatctgcg
      541 gcaggatgac cccgagcagg ttgttggtca gtccgaggtt cttcaccagc aggtacagcg
      601 gcgtgatcgc gacggtcatc gggaacatca gtcccgcggc gaacagcgca tacatcgcat
      661 tgcggccggc gaagctgtag cgggcgatca cgaagctggc catcagggcc agtgcgacgg
      721 ccaccacggt cgtggtgatc gccgagatca tcgagttgcc gaattgctgc cagaacaacc
      781 cgctctgcaa cacggaggtg tagttcgtca gcacccacgg gtcgggccag ccggccgggc
      841 tgaccgtgat ctgcgagttg ctgcggaagc cgccgatgat gatgtacagc acgggggcga
      901 tgcagataga gatcaggacc atcgccacga ggtacgtgac cggactgccc caactggcac
      961 cggagctggt tgtcgtgttg cggcgaccgg tggtggtggc acgtccggtg agggctacct
     1021 gggacatcaa ttctgcttct ttcctggagc ctcggtcagg gcacccgcgg tgtcgcgacg
     1081 caggacgaac ctctggtagg tcaacgcgat ggcgagcgag atgacgaaga tgaccactgc
     1141 aacggcgttg ccgaagccgt agttccccgc aagcctgccc tcacgcacca tgtaggtcgc
     1201 catcgtcgag gtccccgcag tggaggcgac gtactggccc cagatgatgt agacgaggtc
     1261 gaacagctgc agcgaaccga tgatcgagag gaaagcccag acccggatgg tcgggccgag
     1321 cagcggcaac gtgatccagc gctggatctg ccagtacgag gcgccgtcga tggctgctgc
     1381 ttcgtagagc tcctcgggaa tgccctggat gccggcgagc atcaggatga cggcgaagcc
     1441 gatgtacttc caggagatga tcaccatcaa cgtccagatg gcgatgttcg ggtcggcgat
     1501 ccagtcgtgg gcgaaggcgc cgagccccat gttcgtgagc aggtcgttga gggcgccggt
     1561 gtactgcagc atcaggcccc agcccgtacc gacgatgact tccgagatca cgtaggggac
     1621 gaagatcagg acccggatca gggacctacc ccggatgttc tggttgagca ggagggcgaa
     1681 cgcaaccgcg gccgggccct gcatcacgag ggacatcacg aggatgaagc cgttgtgcgt
     1741 cagggcgtcg cggaagttct ggtcgttgaa gatcgtgacg tagttgccga atccgacgaa
     1801 gttcgtgggg acgccgaaac ccttccaccg gaagaagccg tagtaggcag ccatgaacac
     1861 agggaagatg acgaaggcaa ggaacatgac gatcgccggt ccggagagga ccgcgatctc
     1921 gaagcgcttc cgccagtc
//
LOCUS       DASTMD010000180         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_195797, whole genome shotgun sequence.
ACCESSION   DASTMD010000180 DASTMD010000000
VERSION     DASTMD010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195797"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFQ54_02705"
     CDS             complement(<1..703)
                     /locus_tag="VFQ54_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0163925.1"
                     /translation="MTSLESLPATLDPSVTTRVLEAIEAQKDDLIAIVQNLVRIPSEN
                     PKLVDTTVAGEADVQDYVATILAEIGATADRWEPLPGRPNLVGTIAGSGGGHSLAING
                     HIDVVPAGNHDDWARSPYSGEIAEGKIWGRGAFDMKGGVGSMLFAALVLKRLGITLKG
                     DLFLESVIDEETGGPGTRATIERGYRPEFALVVEPTSNLAVIPVEGGLEWLRVTVTGI
                     SGHSAWRYKSVHAGGQ"
     gene            complement(989..1897)
                     /locus_tag="VFQ54_02710"
     CDS             complement(989..1897)
                     /locus_tag="VFQ54_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0163926.1"
                     /translation="MSEASLPATTTVTLASSTAMRSNGAKNRMWHRRLRRDKVAMIAF
                     GILLAFVILAICAPILPLADPIKTDPRQKALAIGSAGHLLGTDQVGRDILARIIWGAR
                     TTLMLGVSAALMSTITGLIFGLIAGYFGNWVDTVIMRIVDVMLAFPSILFAIVIVAIL
                     GPSLRNALLAIALLGVPFYIRLVRAEVLTLRTSDFITAARTIGAPSWRILARHLLPNI
                     LAPIIVTSSIHIGTLILEAGGLSFLGLGAQPPSPEWGAMVASGRNYLLLAPHVVLVPS
                     VVIFIVVLAFNLLGDGLRDALDPKSN"
     gene            complement(1928..2887)
                     /locus_tag="VFQ54_02715"
     CDS             complement(1928..2887)
                     /locus_tag="VFQ54_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0163927.1"
                     /translation="MAGYILRRLTQMIPVLILTTIFVFSMLHLAPGDPVSMILNSKSR
                     PYTQEDFDRTAHDLGMDKPLPVQYLDWVGGVLTGNFGTSIFMRRSIGPILVQRMGATA
                     LLALGALIFALPIGIGGGIFSALWRGSIWDKVINAVIVGGIAFPTFALGLFLIVLFGL
                     EWKILPVGGMTSFGDGSLVDRLKHLVLPAVSLGVGPAAIIARLTRSAMLEVLNQDYIR
                     TARAKGLKYSVVVREHAFRNVLVPVLTVVGLQVGFLLGGAVLVEIVFSWPGMGELIVN
                     GIQQRDFPVVQAGVLVIATMFILINMLVDVIYAVVDPRVRYAK"
BASE COUNT          617 a         1016 c         1000 g          498 t
ORIGIN      
        1 cctgtccccc cgcgtggacg ctcttgtagc gccaggcgga gtgaccggag ataccggtca
       61 cggtcacccg cagccactcc agtccccctt cgaccggtat cacggcgaga ttggacgtgg
      121 gctcgacgac cagcgcgaac tcgggtcggt agccgcgttc gatcgtcgcc cgcgttccgg
      181 gtccacccgt ttcctcgtcg atcacgctct cgagaaagag atcgcccttg agggtgatgc
      241 cgagtcgctt caggaccagg gcggcaaaga gcatcgaacc gacgccaccc ttcatgtcga
      301 aggcgcctcg accccagatc ttgccctcgg cgatctcccc ggagtagggg gaccgggccc
      361 agtcatcgtg attcccggcc ggaacgacgt cgatgtgacc gttgatcgcc aggctgtgcc
      421 cgccaccact tcccgcgatc gtcccgacca gattcggccg gccgggcaac ggttcccaac
      481 gatccgcggt ggcgccgatc tcggcgagaa tcgtcgcgac ataatcctga acgtcagctt
      541 ccccggcgac ggtcgtgtcg accagcttcg gattctcgga ggggatgcgg accagattct
      601 ggacgatggc gatcaggtcg tccttttgcg cctcgatcgc ttcgagaacc cgtgtggtaa
      661 cggaaggatc cagcgtagcg ggtagtgatt cgagtgaagt catgaagcga aatcctttca
      721 tacaacagga tgacagtggg gacggagaag tcctcccgta cgaacacacg ccgtgcccaa
      781 cgcgatcgtg catacggcgg gacctccgtg tcccgcctcc gtgtccgtcc gccatgtgct
      841 ggcaccgcgc tcgcgacgtt catatcgggc tgcgcccgat cggtgcgctg ccgggccaga
      901 ccagctgacc cgctgcggag ataatggacg tcaatgacgt cgtcgtttgt accgcgcgac
      961 acaggttcgc gcgccaacac gcgattggtc agttcgattt cgggtcgagt gcatcgcgca
     1021 ggccgtcgcc aagcaggttg aatgcgagca cgacgatgaa gatcacgacc gaaggcacga
     1081 gcacgacgtg cggcgcgagg agcaggtagt tccggcccga tgccaccatc gcgccccact
     1141 ccggtgaagg cggttgcgcg ccgagcccga ggaagctcag gccacccgcc tcgaggatca
     1201 gcgtgccgat atggatgctc gaggtcacga tgatcggggc gaggatgttc gggagcagat
     1261 gccgggcgag gattctccag ctcggggcgc cgatcgtccg cgccgccgtg atgaagtccg
     1321 acgtccgcag cgtcagcact tccgcccgca cgagccggat gtagaacggc acgccgagca
     1381 gcgcgatcgc caggagcgcg ttccgcaggc tcggcccgag gatggcgacg atcacgatcg
     1441 cgaagaggat cgacggaaac gcgagcatca catcgacgat ccgcatgatc accgtgtcga
     1501 cccagttgcc gaaatagccc gcgatcaatc cgaagatgag gccggtgatc gtggacatca
     1561 gcgccgcgct tacgccgagc atcagcgtcg tccgggcgcc ccagatgatg cgggcgagga
     1621 tgtcgcgacc aacctgatcg gtaccgagca ggtgccccgc gcttccgatc gccaacgcct
     1681 tttgccgggg gtcggtcttg atcggatcgg cgagcggcag gatcggcgcg cagatcgcca
     1741 ggatcacgaa ggcgagcagg atgccgaacg cgatcatcgc gaccttgtcc cgccgcagcc
     1801 ggcgatgcca catccggttc ttcgcgccgt tcgatcgcat tgcggtagac gaagccaggg
     1861 tgactgtcgt cgtggcggga agcgacgcct cactcatagc catgcccgcc tttcactgga
     1921 gcccacatca cttcgcataa cgcacccggg gatcgacgac ggcgtaaatg acatccacca
     1981 gcatgttgat gagaatgaac atcgtcgcga tcacgagcac gcccgcctgc acgaccggga
     2041 agtcgcgctg ctggatgccg ttgacgatca gttcacccat cccgggccag gagaagacga
     2101 tctcgacgag caccgcgccc ccgagcagga aaccgacctg gagcccgacg acggtcagta
     2161 cgggcaccag cacgttccgg aaggcatgct cgcggacgac cacgctgtac ttcagcccct
     2221 tcgcccgtgc cgtgcggatg tagtcctggt tcaggacttc gagcatggcg gagcgcgtca
     2281 gccgcgcgat gatcgccgcg ggaccgacgc cgagactgac cgcgggcagg acgaggtgct
     2341 tcaggcgatc gaccagactg ccgtcgccaa agctggtcat gccgccgaca ggcaggatct
     2401 tccattcaag accgaagagc acgatcagga agagccccaa cgcgaacgtc ggaaaggcga
     2461 ttccccccac gatgaccgcg ttgatcacct tgtcccagat cgatccccgc cacagggcgg
     2521 agaatatccc tcccccgatg ccgatcggca acgcgaagat gagcgcgccg agcgcgagca
     2581 gcgccgtcgc tcccatccgc tgcaccagga tcggaccgat cgagcgccgc atgaagatcg
     2641 acgttccgaa attgccggtc agcacgccac cgacccaatc gaggtactgg acgggaaggg
     2701 gcttgtccat cccgaggtca tgagccgtac ggtcgaaatc ctcctgggtg tacggtcggg
     2761 atttcgagtt caggatcatc gagaccgggt cgcctggcgc caggtgcaac atggagaaga
     2821 cgaaaatcgt cgtcaggatc aggaccggga tcatctgggt cagtcgccga agaatgtagc
     2881 cagccatgat gtgaccctcc acgatggaaa tgaacgtcgc gatgacggcg tgcgccactc
     2941 gcggtcggag cccgcgcccg gcgtgcgatc gggctccaga acagccagcg actggctcgg
     3001 gatggacgtg cggtgcatca gtcggaaacg gtgacgcctt cgaggatgag atccatgctc
     3061 ggcaccgccg cgaatccctg cacccgcttc gatgtcccga agagctgctg gcgatggcag
     3121 accggcacga t
//
LOCUS       DASTMD010000181         6474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2070555, whole genome shotgun sequence.
ACCESSION   DASTMD010000181 DASTMD010000000
VERSION     DASTMD010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2070555"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1924
                     /locus_tag="VFQ54_02720"
     CDS             122..1924
                     /locus_tag="VFQ54_02720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0163928.1"
                     /translation="MTRTIDQPATDIIVFWIAGMASFTLAASWGFWNLMGGLPRIRVS
                     RHSISLATGTGIAILAAASVARFWDLARYPTILDSDEGFFMTAARSARHGDLPNPFHL
                     RFFSVPELYLAVEGLISRPFPDDVASYRILSAFCGVLTVYATWRIGRRMLGEAIGLMG
                     AAILAFMPLHLWASRNGMNNVFDSTTLALPILFVDRAIVRRSRLDALICGLILGFGQY
                     GYYGARIFLPILLVCLAVAVVLPTTRISILEAGRLGLWILVGFASSSAPLIAHYIANP
                     SDFWARINAVSADNGANDPSLIDRAANIGRAVIYPIIDENRGGGIGAGFWHGLNPMLG
                     WAIAPLIAIGGVTWLVWLVLGWVRPGTGKPRTAVLLVVWAAICAGVAQTVEMQSQRFL
                     AVTWIWALAAATGIAVIGMAIQRVAPVPVPMPWMRVAGAVAVIAIAIWHAQFFFSEDR
                     QVEAYGDDRTLAAYDLGTRFEHLDPATYPQVIVGSPGSFNYNGFGNWILLAPDLVTDV
                     TELPWDQDFINDPPLMQPDQLLVMSAERPLPELCSIQRRNPQSHIGEAFSRTGVPLYF
                     LFSTGRDLILPTGETPGQTSLQPVESLDCQGIAA"
     gene            1921..4182
                     /locus_tag="VFQ54_02725"
     CDS             1921..4182
                     /locus_tag="VFQ54_02725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0163929.1"
                     /translation="MSEGVPGTDGGNQTTGAERHVHEAVSAGHSPYPRRPRWTSLNTL
                     SPSTVKTRTVLATARAQRQSSWSAIALIIGAAAIVLAQLVMISDRSHATGIGFLIYAF
                     GVVLIGLAGWSRLWSYAGGVPAADTRATDRTSPAWLIGLGIASTAIAIGRAWHRGTNQ
                     SVNDIVVYWFAAIAFFVIAACWGMWEGKRITLPLRITFRTPAVTWPLIAGCAILIVAA
                     FARLWELDRFTTIHDSDQGYYSIQARAFRDGKIANPFHLFFFTVPQLYVAVEGWLSRP
                     FAESWAIYRVPSAVLGIVSVYATWRIGRRLLGDGIALMGAAILAVMPLNLWASRVSQN
                     NIVDCAALALGILFIDRAIAHRSRLSALVCGLILGFAMYGYYGSRIFLPIIFVCLVAA
                     IILPGSRLPILEAIRLAGWSALGFVSAAAPIIAHYLDHPRDFTARIDAVTKAKGHGPT
                     LLDRAEDIGKALIYPMLDEGRGNYMTGFWRGLNPMLGWALAPFIAIGGVTWIVWLIRE
                     WIHPRRERPRPAFLLIAWGVICTGVAQTPDMQSQRFLAVTWVWALAAATGIAVIGMAI
                     QRLMPEPVPIPWMRVAGGVAVAAIAIWHAQFFFNEDRQIGAYGDVRSMATYDLGWRFH
                     QLDSSSYPRVIIASPSKFTYSGFGNWIFDAPALGPLVTNVPLDIDLIANPPVLEPGEL
                     IVISPELPKAQICSVANRNPTATVGEGFNRAGTLLYIVFSSSTDLILPTNTSPGGTVL
                     RAIDTLDCQEPQS"
     gene            4179..5123
                     /locus_tag="VFQ54_02730"
     CDS             4179..5123
                     /locus_tag="VFQ54_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU0163930.1"
                     /translation="MSERILVAGGAGFIGKHLCRALVAQGHRVFCLDNFSTSDPMARH
                     ELEDIGVHVDEADVTMAIDRPYDAIYHLASPASPVHYRRMALSTMWANALGTKRLVEL
                     ANASGGRFLLASTSEIYGDPLVHPQTEDYWGNVNPIGERACYDESKRFAETLTWEYRR
                     VYNVNARIVRIFNTYGPGMMLDDGRAIPEFTVSALKGRPIRIQGSGEQTRSFCYVDDL
                     VRGLLLVGQDPKADGQVYNLGSPGEFTVSELANLVKEISGSSSEIVYGDAVPDDPKRR
                     KPNIDRIKAAYNWEPTTVLRDGLAPTIADIASRLDTAS"
     gene            complement(5548..>6474)
                     /locus_tag="VFQ54_02735"
     CDS             complement(5548..>6474)
                     /locus_tag="VFQ54_02735"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEU0163931.1"
                     /translation="ISGQDHLDASLLLGLILIIVGLGFKMAAVPFHFWTPDAYDGAPT
                     PVSAYMSVVPKAAAVAAAIRILVQGLAPLRDDWVTLVAILAVITMIFGNIVAISQRNI
                     KRMLAYSSIAHTGYIMAGLAAYRVSDGFSAGSSSSAAISSVLYYMLAYTFMNIGAFAI
                     MGWMQQRGRGMMLEDFNGLASQHPLPAAAMAIFLISLMGVPPTIGFYGKYYVIVALIN
                     SDLLWLAIAIVVVTAISAFFYLRVAAAMYFSEAKETEYEKRFDGVRTPLLDTGIGVMV
                     AAVILLGLFSSPIVNLADQWTTALTLTAVVHP"
BASE COUNT         1090 a         2158 c         2056 g         1170 t
ORIGIN      
        1 ccggacattc attccctcgc cgcattccgc aatccaggct ccgccgatcg cccattcgca
       61 ggtcccggca tggttgattg gcaccggaat cttctgctct ctcgtcacct acgaacgagc
      121 cttgacgcgc acaatcgacc aacccgcgac agacatcatc gtcttctgga tcgccggcat
      181 ggcgtcattc acgctcgctg cttcctgggg attctggaat ctgatgggcg ggttgccgcg
      241 tatccgcgtc tcccggcact cgatatccct ggcgacggga accgggatcg ccatccttgc
      301 cgccgcatcc gtcgcacggt tctgggatct cgctcgctat cccacgatcc tcgacagcga
      361 cgagggattc ttcatgaccg cggcccgcag cgcccgtcac ggggacctcc cgaacccgtt
      421 ccatctcagg ttcttctcgg ttccggagct ctacctggcc gtcgagggac tgatctcccg
      481 gccgttcccg gacgacgtcg cttcctaccg gatcctcagc gcgttctgcg gcgttctcac
      541 ggtctacgcg acgtggcgga tcggtcgccg gatgctgggc gaggcgatcg gcctgatggg
      601 agcggcgatc ctcgccttca tgccgcttca cctgtgggcg agccggaatg gcatgaacaa
      661 tgtcttcgac agcacgacgc tcgcgctgcc aatcctcttc gtcgaccggg cgatcgtccg
      721 gcggtcccgt ctcgacgcgc tgatctgcgg gctcatcctg ggcttcggcc agtatggcta
      781 ctatggcgcc aggatcttcc tgccgatcct tctcgtttgc ctcgcggtcg ccgtcgtact
      841 ccccacgacc cggatctcga tcctcgaggc aggtcgcctc ggtctctgga tcctggttgg
      901 attcgccagt tcctcggccc ccctcatcgc tcattacatc gccaacccga gtgatttctg
      961 ggcccgtatc aacgcggtct ctgccgacaa cggggcaaat gacccgtcgt tgattgaccg
     1021 cgccgccaat atcggacgcg ccgtcatcta tccgatcatc gacgagaatc ggggcggcgg
     1081 aatcggggcc ggattctggc acggtctcaa tccgatgctt ggctgggcaa tcgcccccct
     1141 gatcgcgatc gggggcgtca cgtggctcgt ctggttggtg ctgggatggg tgcgaccggg
     1201 taccggcaaa ccgcgaacgg cggttctgct cgtcgtatgg gcagcgatct gcgccggcgt
     1261 cgcgcagacg gtagaaatgc agagccagcg cttcctcgcc gtgacgtgga tctgggcgct
     1321 ggcagccgcg accgggatcg ccgtgatcgg gatggcgatc caacgcgtgg ctccggtacc
     1381 ggtgccaatg ccatggatgc gtgttgccgg cgcggtcgcc gtcatcgcga tcgcgatctg
     1441 gcacgcgcag ttcttcttca gcgaagaccg tcaggttgaa gcctacggtg acgatcgcac
     1501 gcttgccgcc tacgacctcg gcactcgctt cgagcacctc gatcccgcga cgtatccgca
     1561 ggtaatcgtg gggagtcccg gcagcttcaa ctacaacggg ttcgggaact ggatcctcct
     1621 cgcgccggac ctcgtgaccg atgtgacgga actcccgtgg gaccaggatt tcatcaacga
     1681 tccgcccctg atgcaaccgg atcagctgct cgtgatgtcc gccgagcgtc cgctgcccga
     1741 actctgctcg atccaacggc ggaatccgca atcccacatc ggcgaagcct tcagccggac
     1801 gggcgttccc ctctacttcc tcttcagcac cggacgtgac ctgatcctcc cgaccggtga
     1861 aacacccggg cagaccagcc tgcagccagt cgagtccctc gactgccagg ggattgccgc
     1921 atgagcgagg gggttccagg gacagacggc ggaaaccaga cgactggcgc cgaacggcac
     1981 gtgcacgagg cagtgagcgc tggccactcg ccatacccac gccgcccgcg ctggacgagc
     2041 ctgaacacgc tctccccatc gaccgtgaag acgcggacgg tgctggcgac tgccagggcg
     2101 cagcgtcagt ccagctggag cgcgattgcc ctgatcattg gcgcggcggc catcgtgttg
     2161 gcgcagttgg tgatgatctc ggaccgctcg cacgcgactg gaatcggctt cctgatctat
     2221 gcgttcggtg ttgttctgat cgggctcgcc ggctggtcgc ggctctggtc gtacgccggt
     2281 ggcgtgccgg cggcagatac gcgcgcgacc gaccgcacat caccggcgtg gctgatcggc
     2341 ctcggcatcg cgagtaccgc gattgcgatc ggtcgcgcct ggcacagggg caccaaccaa
     2401 tccgtcaacg acatcgtcgt ctactggttc gcggcgatcg cgttcttcgt catcgccgcg
     2461 tgctggggga tgtgggaggg gaagcgaatc accctgccgc tccgcatcac gttccggacc
     2521 ccggcagtca cctggccgct gatcgccggt tgcgcgatcc tgatcgtcgc ggccttcgcc
     2581 cgcctctggg agctcgaccg cttcacgacg atccacgaca gcgaccaggg ctactactcg
     2641 atccaggcac gcgccttccg ggacgggaag atcgcgaatc cgttccacct cttcttcttc
     2701 acggttcccc aactctatgt cgccgtcgaa ggctggctct cgcgaccctt cgcggagagc
     2761 tgggcgatct accgcgtacc gagcgccgtc cttggcatcg tcagcgtcta cgcgacgtgg
     2821 cgcatcgggc gacgcctgct cggggatggc atcgcgctga tgggcgcggc gatcctcgcc
     2881 gtcatgccgc tgaacctgtg ggcgagccgc gtctcccaga acaacatcgt cgactgcgcg
     2941 gcgctggcgc tgggaatcct cttcatcgat cgcgcgatcg cccatcgctc ccgcctcagc
     3001 gcgctcgttt gcggactcat cctcggcttc gcgatgtacg gctactatgg ctcgcgaatc
     3061 ttcctgccga tcatcttcgt ctgcctcgtc gcggcgatca tcctgcccgg cagccgattg
     3121 ccgatcctcg aggcgatccg cctcgccgga tggtccgcgc tcggattcgt cagcgcggcg
     3181 gcgccgatca tcgcccacta cctggatcat cccagggact tcactgcgcg gatcgatgcg
     3241 gtcacgaagg cgaagggaca cggccccacc ctgctggacc gggccgagga catcgggaag
     3301 gcgctcatct acccgatgct cgacgaagga cgaggcaact atatgaccgg attctggcgc
     3361 ggcctgaatc cgatgctcgg ctgggcgctc gcccccttca tcgcgatcgg tggcgtgaca
     3421 tggatcgtct ggttgatccg ggagtggatt catccccgcc gggaaaggcc gcgaccggcg
     3481 ttcctgttga tcgcctgggg cgtcatctgc accggcgtcg cgcagacgcc tgacatgcag
     3541 agccagcgct tcctcgccgt gacgtgggtc tgggcactgg cggccgcgac cgggatcgcc
     3601 gtgatcggga tggcgatcca gcgcctgatg ccggaaccgg tcccgatccc ctggatgcgc
     3661 gtcgccgggg gtgtcgccgt cgcggccatc gcgatctggc atgcgcagtt cttcttcaac
     3721 gaggaccgcc agatcggcgc ttatggcgac gtgcgctcga tggccaccta cgatctcggc
     3781 tggcgcttcc atcaactcga cagcagctcc tacccgaggg tgatcatcgc cagcccgtcg
     3841 aagttcacct actccggctt cgggaactgg atcttcgatg ccccggccct gggacccctg
     3901 gtcaccaacg ttccactgga catcgacctg atcgccaatc cgccggtact cgagccggga
     3961 gagctgatcg tgatctcgcc ggagcttcca aaagcgcaga tctgctcggt cgcgaaccgg
     4021 aatccaaccg ccacggtcgg cgaggggttc aaccgagcgg gtacactcct ctacatcgtg
     4081 ttcagttcca gcaccgatct cattctcccg accaacacgt ctccgggcgg caccgtcctg
     4141 agagcgatcg acaccctaga ttgtcaggag ccccaatcgt gagcgagcgt attctggtgg
     4201 ccggcggggc cgggttcatc ggcaagcatc tgtgccgggc actcgtcgcg cagggacatc
     4261 gtgtcttctg cctcgacaac ttctcgacgt ccgatcccat ggcgcggcat gaactggaag
     4321 acatcggcgt ccacgtcgat gaagccgatg tgacgatggc gatcgaccgc ccgtacgatg
     4381 cgatctatca cctcgcctca ccggcgagcc cggtccacta ccggcggatg gcgctctcga
     4441 cgatgtgggc gaacgcgctc ggcacgaagc ggctcgtgga actcgcgaat gcgagcggtg
     4501 gtcgtttcct cctcgcctcc acctccgaga tctacggcga tcccctcgtc cacccgcaga
     4561 ccgaggacta ctgggggaac gtcaatccga tcggcgagcg cgcctgctac gacgaatcga
     4621 agcgattcgc ggagaccctg acgtgggaat atcgccgcgt ctacaacgtc aacgcgcgga
     4681 tcgtccgcat cttcaacacc tacggcccgg gcatgatgct cgacgacggc cgcgcgatcc
     4741 cggagttcac cgtttccgcg ctcaagggcc gcccaatccg gatccaggga agcggcgagc
     4801 agacgcgatc cttctgttat gtcgacgacc tcgtgcgcgg gctgttgctg gtcgggcagg
     4861 acccgaaggc ggacggccag gtctacaatc tcggctctcc gggcgaattc acggtcagcg
     4921 agctggcgaa tctggtgaag gagatctccg gcagctcgag cgagatcgtc tacggcgacg
     4981 ccgttccgga cgatccgaag cgacgcaagc cgaacatcga ccggatcaag gccgcctaca
     5041 actgggaacc gacgaccgtg ctccgcgacg gcctggcgcc gacgatcgcc gacatcgcga
     5101 gccggctcga caccgcgtca tgacgtcgtg agggcagatt ccgaagtgaa gtggttgatc
     5161 ggctgcacgg agccggcgat gggtgcggcg ctgtcgaccc atcgcccgga atggcaggcc
     5221 gcgatccaca catcgagatg cacagcggtc gttcgtacgg cgggacctcc gtgtcccgcc
     5281 tcgaccatgc cgcgagacca ttcccgtccc cacggcgctt gtcgccgttg atgcggcgtt
     5341 ggggcgggag cacgtggatg cagaccgatc cctcgacagg ctcgggaaga cgttcatggg
     5401 acgggtcgct cggtgaacat gggggaggca aaggaaaggg cgaacctggt gttcgcccgg
     5461 acatccggtc gttcgtaggg cgggaccgcc gctgccggac ctcagtccgg acggcctgtt
     5521 ccgcctctcg ttcgcccatg ggttgctcta cgggtggacg acggcggtga gggtgagggc
     5581 ggtcgtccac tggtcggcga ggttgacgat cgggctcgag aagagaccga gcaggatcac
     5641 cgccgcgacc atcacaccga tgccagtatc gagcagcggc gtgcggacgc cgtcgaagcg
     5701 cttctcgtac tccgtctcct tcgcctcgct gaagtacatc gcggcagcga cgcggaggta
     5761 gaagaaggcg ctgatcgcgg tgacgacgac gatcgcgatc gccagccaga gcaggtccga
     5821 gttgatgagg gcgacgatca cgtagtactt gccgtagaag ccgatcgtcg gcggcacacc
     5881 catcagggag atcaggaaga tcgccatcgc cgccgccggc agcggatgct gcgaggcgag
     5941 accgttgaag tcctccagca tcatcccgcg tccacgttgc tgcatccagc ccatgatcgc
     6001 gaaggcaccg atgttcatga aggtgtaggc aagcatgtag tagagcacgc tcgagatcgc
     6061 cgcactggat gagctaccgg ccgagaaacc gtcgctcacc cggtacgcgg cgagaccagc
     6121 catgatgtag ccggtatggg cgatcgatga gtaggcgagc atgcgcttga tgttgcgctg
     6181 ggagatcgcg acgatgttgc cgaagatcat cgtgatcacg gcgagaatcg cgacgagcgt
     6241 cacccagtca tcgcgcagcg gcgcgagccc ctggacgagg atgcggatcg cggcggcgac
     6301 ggcggccgcc ttcggcacga cggacatgta ggcggagacc ggtgtcggcg cgccgtcgta
     6361 ggcatccggc gtccagaagt ggaatggaac agccgccatc ttgaagccga gaccgacgat
     6421 gatcaggatc agtccgagca gcaatgaggc gtcgagatga tcctggccgg agat
//
LOCUS       DASTMD010000182         2742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2337740, whole genome shotgun sequence.
ACCESSION   DASTMD010000182 DASTMD010000000
VERSION     DASTMD010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2337740"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFQ54_02740"
     CDS             <1..353
                     /locus_tag="VFQ54_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0163932.1"
                     /translation="LDALIAPTAPVPAVIDSYGDAGFTGSSAQISALAGYPAINLPIG
                     LVRELPVGMNIFGRAFSEPTLLQIAYGFEQILQARTPPTYIPTIGPDPTPESEASPAD
                     DDTPVDEDTPADGE"
     gene            859..2433
                     /locus_tag="VFQ54_02745"
     CDS             859..2433
                     /locus_tag="VFQ54_02745"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU0163933.1"
                     /translation="MLDAEIRNASVAELRAALDAGELTSRALVESCLARIEALDRQGP
                     TLGAVIEINPDALTIADALDAELKTTGARGPLHGIPILLKDNVATIDPMETTAGSLAL
                     VGARPVKDAFLAARLREAGVVILGKANLSEWSNFRSTRAVSGWSGRGGQAVNPHQLDR
                     SSSGSSSGPGVAVAAGYVPFAVGTETNGSIVSPAGSNGIVGIKPTIGLVSRQGVVPIS
                     HNQDTAGPMARSVADAAVLLQVLAAPDPDDLASHEGERPKDVPSYPARPARISAGEAD
                     YVAALDADGLRGARIGVLRRQEGPGWVPGDPILLDIIATLKDAGAEVIDPVLIPEGAE
                     QKWGVSNIDNMLWEFKATIADYLATFAPDFPIKDLAGLIAFNNEHAEEEMPWFGQDLF
                     ERSEAMTGLDDPAYIELEMRLQRNSRELGLDAMLAEHNLDAIVALTNGPAWKIDPLLG
                     DRPSAGSSGLAALAGYPLVTLPAATYGGMPIGLTFMAGAYSEETLIRLAYSFEQRTHA
                     RRDPQFLPGAVYPAEK"
BASE COUNT          443 a          911 c          914 g          474 t
ORIGIN      
        1 agctggacgc gctgatcgcg ccaaccgctc cggtgccggc ggtgatcgat tcctacggtg
       61 atgccgggtt cacaggatcg tccgcccaga tttctgccct cgcggggtat cccgcgatca
      121 atctgccgat cggtctcgtc cgtgagcttc cggtcgggat gaatatcttc gggcgcgcgt
      181 tcagcgagcc gacgctcctc cagatcgcct acggcttcga gcagatcctg caggcgcgaa
      241 ccccaccaac ctacatccct acgatcgggc ccgatccgac gccggaaagc gaggcttcgc
      301 ccgccgatga tgacaccccg gtcgacgaag acactcctgc cgacggcgag tgaggtgacc
      361 cgtccaacca cgacgggatc tcgcgggtcg tcatggcatc gaccgggatg tttcacgata
      421 tccgcgccgc gtcatttcga gcttgtcgag aaatccccgc gcgaagcgca ctcgccgcag
      481 gcgggctcaa acgggaacat tggagcgagg ttcaggtcgg cggcgacacc ggttgtcgca
      541 ctccgttcaa atgatcggcg ttcgcggcaa gacacaaggt cttgccctac agacccgggg
      601 gtgtcgacac cggggcgagc gacccgaact gcgttgtagg atcgcgttgg cgaccggcgg
      661 cgtttccccc tgatcctgtc gcatgggccg cgtctccggt ttcatggaag aacgagcaag
      721 gcataagcgc ttgcgatcgt ttgtgcctag tgcacagggg acgtgtcgct ccggacctat
      781 cccggcgcga gatcgctgcg tacaatcccc tccagtacgt cgccgtcctt caaatcacct
      841 attgaaaggc agacatccat gctggatgcg gagatcagga acgcctcggt cgcggaattg
      901 cgcgcggcgc tggatgccgg ggagctgacc tcgcgcgcgt tggtcgaaag ctgcctcgct
      961 cgtatcgagg cactcgatcg ccaggggccg acgctggggg ccgtgatcga gatcaatccc
     1021 gacgccctca cgatcgccga tgcgctggac gccgaactca agacgaccgg cgcgcgcggc
     1081 ccgctgcacg ggattccgat tctgctcaag gacaacgtcg cgacgatcga tccgatggag
     1141 acgacggcgg ggtcgctggc gctcgtcggt gcgcgaccgg tgaaggacgc cttccttgcc
     1201 gcccggctgc gggaagcggg tgtcgtcatc ctcggcaagg cgaacctgag cgagtggtcg
     1261 aactttcgct cgacgcgcgc ggtcagcggt tggagtggtc gtggcgggca ggcggtgaat
     1321 ccgcatcaac tcgatcgttc gtcctcggga tcgagttccg gaccgggtgt cgccgtcgcg
     1381 gcgggatacg tgcccttcgc ggtggggacc gagaccaacg gctcgatcgt ctcgccggcg
     1441 ggctcgaacg gcatcgtcgg aatcaagccg acgatcggcc tggtcagccg ccagggtgtg
     1501 gtcccgattt cccacaacca ggacaccgcc ggtccgatgg cacgctccgt cgccgatgcc
     1561 gcggtcctgc tccaggtcct cgccgcgccc gatcccgacg atctcgcctc gcacgagggc
     1621 gagcggccga aggacgtccc ctcctacccc gctcgcccgg cgcgaatctc ggctggtgag
     1681 gccgattatg tcgcggcgct cgatgcggac ggtctccgtg gcgcccggat cggtgtcctg
     1741 cgccggcagg agggcccagg ctgggtgccg ggcgatccga tcctcctcga catcatcgca
     1801 acgttgaagg acgctggcgc cgaggtgatc gatccggtcc tgattccgga gggcgccgag
     1861 cagaagtggg gcgtttccaa catcgacaac atgctctggg agttcaaggc gacgatcgcc
     1921 gactacctcg cgaccttcgc gcccgacttc ccgatcaagg atctcgccgg gctgatcgcg
     1981 ttcaacaacg agcatgccga ggaggagatg ccctggttcg ggcaggacct cttcgagcgt
     2041 tcggaggcaa tgaccggcct cgacgatccc gcctatatcg aactcgagat gcgcctgcaa
     2101 cgcaactcgc gtgagcttgg tctcgatgcg atgctcgcgg agcacaacct cgacgcgatc
     2161 gtggccctga cgaacggtcc cgcctggaag atcgacccat tgctgggcga tcgaccgtcg
     2221 gcggggagtt ccggtctcgc ggctctcgcg ggatatcccc tggtgacgct cccggcggcg
     2281 acctacggcg ggatgccgat cggactcacg ttcatggccg gcgcctattc ggaggagacg
     2341 ctgattcgcc tcgcctattc gttcgagcag cggacgcacg ctcgccgtga cccgcagttc
     2401 cttccgggcg cggtctaccc ggccgagaag taggcagtcc cttggatccg ggcgagcggg
     2461 atcggcactg gccggtcccg ctcgccttgt cttgttggtc atcccgagct tgtcgaggga
     2521 gcagccggcg tccacgtgtt ccccttgcga cgccgcccaa gacacgtcct cccgagcctg
     2581 tcgagggacc agttggcgtc cacgtgtttc cggcacgaca cgcttccccg gagggggcag
     2641 gcacgcgagc catcgcgcat gggacgtggt cccgggcgaa caccaggttc gcccctacgg
     2701 acgggcgcct gacggcggga cgtgtggggc cggaggcgtt gg
//
LOCUS       DASTMD010000183         4169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_196198, whole genome shotgun sequence.
ACCESSION   DASTMD010000183 DASTMD010000000
VERSION     DASTMD010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196198"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..645
                     /locus_tag="VFQ54_02750"
     CDS             235..645
                     /locus_tag="VFQ54_02750"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEU0163934.1"
                     /translation="MRLMDGELVIRVVAMPGDTNPDGDMFGGWVLSQMDLGAYVQARK
                     YTHTKIVTVAIDNIVFHRPVLVGDCLLCYATTERIGRTSITIRIDAMVERKEGRHLEQ
                     VTEGRFVFVAIGDDRKPVPIAQPMESGEDTESVS"
     gene            complement(811..2208)
                     /locus_tag="VFQ54_02755"
     CDS             complement(811..2208)
                     /locus_tag="VFQ54_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU0163935.1"
                     /translation="MSFSPSPRRRTQSVAGTGSPVRGDPARRGKRATTIWRLGFLGSG
                     AIAIAVVLIALLGSVGAQPALAKDAPKYSADYLDNGGSFSISQFKGQVLLLNIWATWC
                     TPCQREIPEVNALDEQFKSQGLKVIGVSIDTTQSDSQIQQFATKHGATYPLAKDPSND
                     FSSIFRTTGVPVTVLIDRAGNIAKLWPGQLTAAEGADNRQIIQQTLDSTGAVDTSTLP
                     KAAAVGFIAAFGAGLLSVLSPCVFPLIPTYAAYITGISVDEMLKQKQEAERRKTRRTA
                     LRNGLFFVLGFSLIFIAMGASASAIGGLLHDYRVWIARIGGLLLLVMGAHMLGLLRIP
                     VLDRIIRPNIGGNMGRTTNPLGTLAVGMAFGAGWTPCIGPALASILTLAAATASVGRG
                     IALLSVYSLGLAVPFLLGTVLIDRFIRRRAGFGPWLPRLERISAVLIVAIGVLMITGF
                     FTQLAAWTSGIPSLA"
     gene            complement(2205..2714)
                     /locus_tag="VFQ54_02760"
     CDS             complement(2205..2714)
                     /locus_tag="VFQ54_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU0163936.1"
                     /translation="MTATATGPRSSDDREAPTAHARELARAAAQDAIRKKARSRVYRR
                     GGYIMIALLLIVTLLSVIYIVTNRHNEDLANSGLQFVIPNGASETVAIPTINSAIAIP
                     TSIVFKPGEPAVLSIRNEDSVANRAGPWVVGPGQTYTAKFDKPGVYKYVCSVNAAESV
                     TITVEGDDS"
     gene            complement(2754..3365)
                     /locus_tag="VFQ54_02765"
     CDS             complement(2754..3365)
                     /locus_tag="VFQ54_02765"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEU0163937.1"
                     /translation="MNDIRLSRRRLVALGAAIPLTGLIAQRVSAAQDDPGATPAASPA
                     ATPTVNTGTAAVYFKIQNNASNQDTLTGATTTAAQSCEIHNMVMEGNVMSMIPVPSGV
                     EIPAGGMLSFDPNSYHLMLVNLNHDLLPGSTFEVTLHFQNAGDVSITVSVTTDTPDDS
                     VEFTTGHITVQAPWCLPAPMITAGNDMSGMDMGTPAATATPGM"
     gene            complement(3398..4030)
                     /locus_tag="VFQ54_02770"
     CDS             complement(3398..4030)
                     /locus_tag="VFQ54_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU0163938.1"
                     /translation="MFKSRQKNIIFGAGTVVLVLIVALGAWLAFFRSDSAYAYKGGFY
                     EPPNAASPLNLVDQNNKPFTLDQVKGKVTLLYFGYTYCPDFCPTTLTDFGKVKKELGT
                     QASDVDVVFVTVDPDRDTPARMKQYLAFFDPTFIGLYGKQDQLTPIEQAYGIQVVKDA
                     ATPGTDYYSVSHSTSLYAIDQQGNLRLTWPYGTTPEDITSDVKHLLSSGS"
BASE COUNT          757 a         1280 c         1386 g          746 t
ORIGIN      
        1 gagatcttga tgcccgaatc ccggccacat tcgctcccca acgcagacta cgtgccacat
       61 ggaacagatc ctttacgcca aataatggct tatgcatgaa acaccatacg gatatctcgc
      121 agccgcgcgc tcatccaacc cggcgacagc gcctgggcgc ctcgacgctc gggacaatga
      181 cgggtaaggt gcgtcgttgt gcgtgatccg ataggtgagg aatcgattgg gagcatgagg
      241 ctgatggatg gtgaattggt gatccgtgtc gtcgcgatgc ctggtgacac gaatccggat
      301 ggggacatgt tcggcggctg ggtgctcagc cagatggatc tcggcgccta tgtgcaggcc
      361 cggaaataca cgcacaccaa gatcgtgacg gtcgcgatcg acaacatcgt cttccaccgc
      421 cccgttctcg tcggcgattg cctgctctgc tacgcgacga ccgaacgaat cggacgcacc
      481 agcatcacga tcaggatcga cgcaatggtc gagcgcaagg agggccgtca cctcgagcag
      541 gtgacagagg gccgcttcgt cttcgtcgcg atcggcgatg accggaagcc ggtaccgatc
      601 gcgcagccca tggagtccgg cgaagacacg gaatccgtct catagcgaac accgaagcga
      661 ttgccacacg aacgacgcgt cccgccctcc gcacgtcgcg tcgaccggca tcagtcggac
      721 tctcgtcact cgcctcacgc agaaagccct ggaccaatgt ggtccagggc tttcccgtgg
      781 ctttgcgaac cgtccgaacg ccgcggagcg tcaggcgagg gacgggattc ccgatgtcca
      841 cgcggcgagt tgggtgaaga agccggtgat catcaggacg ccgatcgcca cgatcagcac
      901 cgcgctgatc cgctcgagcc gtggcagcca cgggccgaat ccggcccggc gccgaatgaa
      961 gcggtcgatc agcaccgtcc ccagcaggaa cggcaccgcg agtccgagcg agtagacgct
     1021 cagcagggcg atgccgcgtc ctaccgacgc ggtcgccgcg gcgagcgtca ggatgctggc
     1081 cagggcgggc ccgatgcagg gtgtccaccc ggcgccgaac gccatcccca ccgcgagcgt
     1141 gcccagcggg ttggtcgtcc gacccatgtt cccgccgatg ttcggtcgga tgatccggtc
     1201 gagaaccggg atccggagca gcccgagcat gtgggcgccc atcaccagca gcagcaggcc
     1261 gccgatgcgg gcgatccaga cgcgatagtc gtggaggagt cccccgatcg ccgaggcgga
     1321 agcgcccatc gcaatgaaga tcagactgaa cccgagcacg aagaagaggc cgttgcggag
     1381 cgcggtccgc cgcgttttcc ggcgttcggc ctcctgtttc tgcttgagca tctcatcgac
     1441 gctgatcccg gtgatgtagg cggcgtacgt cgggatcagg ggaaagacgc agggcgaaag
     1501 gacgctcagc aagccggccc cgaacgccgc gatgaacccg accgccgccg ctttcggcaa
     1561 cgtcgaggta tcgacggcgc cggtactgtc gagcgtctgc tggatgatct gccggttatc
     1621 ggcaccctcg gccgcggtga gctgccccgg ccagagcttg gcgatgttcc cggcgcgatc
     1681 gatgagcacg gtgacgggaa cacccgtcgt cctgaagatc gacgagaagt cgttcgacgg
     1741 atccttcgcc agcggatagg tcgcgccgtg tttggtagcg aactgctgga tctgggaatc
     1801 ggactgggtc gtatcgatgc tgaccccgat caccttcagc ccctggctct tgaactgctc
     1861 gtcgagcgcg ttgacctcgg ggatctcccg ctggcagggc gtgcaccagg tcgcccagat
     1921 attgaggagc agcacctgcc ccttgaactg cgagatagag aacgatccgc cgttgtcgag
     1981 atagtcggcc gagtacttcg gcgcatcctt cgccagcgcc ggctgcgcgc cgacactccc
     2041 gagcagcgcg atcagcacga cggcaatcgc gatcgctccg cttccgagga atcccagccg
     2101 ccagatcgtt gttgcgcgct tcccgcgtcg cgccgggtcg ccgcgtaccg gcgatcccgt
     2161 tccggcgacg gattgcgtcc gtcgccgggg tgatggactg aagctcatga gtcgtcgcct
     2221 tcgacagtga tcgtcacgga ttccgcggca ttgaccgagc agacgtactt gtagacgcca
     2281 ggtttgtcga atttggcggt gtaggtctga ccgggcccga cgacccacgg tcccgcccgg
     2341 ttcgcgaccg agtcctcgtt acggatcgag aggaccgccg gttcgccggg cttgaagacg
     2401 atcgaggtcg gaatcgcgat cgccgagttg atcgtcggga tcgcgacggt ttccgaggct
     2461 ccgttcggaa tcacgaactg aagcccggaa ttcgccagat cctcgttgtg ccggttcgtg
     2521 acgatataga tcacgctcaa cagcgtcacg atcaggagca gcgcgatcat gatgtagccg
     2581 ccgcgccggt agacgcggga ccgcgccttc ttccggatcg catcctgcgc ggcggcacgc
     2641 gccagctcgc gcgcgtgggc ggtcggcgcc tcgcgatcgt cagaacttcg gggaccagtt
     2701 gcggtggcag tcatgcgacg gtgctcctga tcgaaacgcg ccggatatcc aggctacatg
     2761 cccggggtcg cggtcgcggc cggtgtaccc atatccatcc cgctcatgtc gttcccggcg
     2821 gtgatcatcg gggccgggag gcaccacggc gcctgaacgg tgatgtgacc ggtggtgaat
     2881 tccaccgaat cgtccggcgt gtcggtcgtc acggaaaccg tgatcgacac gtcgcccgcg
     2941 ttctggaaat gcagtgtgac ctcgaaggtc gagcccggca acaggtcgtg gttgaggttg
     3001 acgagcatca ggtggtagct gttcggatcg aaggagagca ttccaccggc cgggatctcg
     3061 acgccggacg gcaccgggat catcgacatc acgttgcctt ccatcaccat gttgtgaatc
     3121 tcgcaggact gcgcggcggt cgtggtggcg ccggtcaggg tgtcctgatt gctggcgttg
     3181 ttctggatct tgaaatagac ggcggcggtg ccagtgttga cggtcggcgt cgcggcggga
     3241 gaagccgccg gcgttgcgcc agggtcgtcc tgggccgcgg atactcgctg cgcgatgagg
     3301 ccggtcagcg gaatcgccgc gccgagcgcc acgagtctgc gacgggagag gcggatgtcg
     3361 ttcatgagat gggcttcctt tcgtctcgtt cggtcgatca ggaaccggac gagagcagat
     3421 gcttcacgtc ggaggtgatg tcttccggtg tcgtgccgta cggccaggtc aggcgaaggt
     3481 tcccctgctg gtcgatcgcg tagagcgatg tcgaatggct gaccgagtag tagtcggttc
     3541 ccggcgtcgc ggcatccttc acgacctgaa ttccgtaggc ctgctcgatc ggcgtcagct
     3601 gatcctgctt cccatagagc ccgatgaagg tgggatcgaa gaaggcgagg tactgcttca
     3661 tgcgcgccgg cgtgtcgcgg tcggggtcga ccgtgacgaa gacgacatcg acatcgctcg
     3721 cctgcgttcc gagttccttc ttcaccttgc cgaaatcggt cagcgtcgtc gggcagaaat
     3781 cagggcagta ggtatagcca aagtagagca gggtcacctt gcccttcacc tggtcgagcg
     3841 tgaatggctt gttgttctgg tcgacgaggt tcaggggcga cgctgcattc ggtggctcgt
     3901 agaacccacc cttgtaggca taggcggagt cgctccggaa gaaggcgagc catgcgccga
     3961 gcgcgacgat aagaacgagt acgacggttc cggcgccgaa gatgatgttc ttctggcgtg
     4021 acttgaacat ggcgagatcc tcttgcacca atcgatcgcg gaggacgggc atcatcgact
     4081 cgcgcacgcg ccatcacgcg accgccgcaa tggcaggtgc gtgtggagtg ggtccaaccg
     4141 gaccgtacca gggcgtgaac gtcgatcag
//
LOCUS       DASTMD010000184         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1445681, whole genome shotgun sequence.
ACCESSION   DASTMD010000184 DASTMD010000000
VERSION     DASTMD010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445681"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFQ54_02775"
     CDS             <1..273
                     /locus_tag="VFQ54_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163939.1"
                     /translation="DQTPEKGGFNLSRWVSPDYDKLNDQQLVEFDDAKRMALIEQASD
                     IVNDEVAECVLFFPKGATGSATTLHNFTPNAYGDFWQAPFVWIDQK"
     gene            complement(340..699)
                     /locus_tag="VFQ54_02780"
     CDS             complement(340..699)
                     /locus_tag="VFQ54_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163940.1"
                     /translation="MAFSEDDMRRLHGWLEQRDDHAIEQWFTELPIMFPDTEANDVID
                     SVTPLSAMLMKRLGLRTTHGELTYLSVQPRVEDQILELGQCLLNELTRRYLTSDPRAG
                     TPLRDPGANEASTASQA"
     gene            complement(1399..2733)
                     /locus_tag="VFQ54_02785"
     CDS             complement(1399..2733)
                     /locus_tag="VFQ54_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU0163941.1"
                     /translation="MKRIHAPRHLAVVLVMFALFVLPSGPAFAAAIPAPAGIVTTSAP
                     IDGATPVLEGPTTTPTGITTPSGTVAPTTTPSEAPTATPTHPATATPPASGVNAAATD
                     CTPGAGKALAACVYDGQDLAGANFSGSDLTGATFNGANVSGSNFSNANLTAATFDGAN
                     LSCSNFNGAVLNATTLNGANLAGSTFDGATFVSVTWGSSTTCPDGTLSGNNGGTCVNN
                     LDAIACGAATVTPTTGPTAVPTATTIPVNTPPGTGPTATVAPGETPPGGLPTSVPTTR
                     AEATGTAGATLPTPTATTAGGSGPVSTGIPPAQTPTPTGGNGDSGELTPTIVGASPQT
                     IPTQVPTGGGGGSTNGQPTPLGGGGSSTGSEGGDTSSNDTTDSNAGQFGSAVNTLASP
                     VSLSGLPNTGSIPAEKTDDITSRMLILLAAIGIVTAFAIRKRRNLTRQDCGR"
     gene            3326..4084
                     /locus_tag="VFQ54_02790"
     CDS             3326..4084
                     /locus_tag="VFQ54_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0163942.1"
                     /translation="MKIWLLRNATLVLDYGERTILVDPMLDPAGARPAVANTPNDLRN
                     PLVELPLPIDEILSGIDALLVTHLHQDHFDSTAKERLAKTLPLIGQPEDVERLTVDGF
                     TNLLPVDDERLWEGIAIRRTRAQHGTGEIAKLMAPVSGFVLEAEGEPTLYLAGDTIWY
                     EPVAAVLATHRPDVIVLNGSGARFNVGDPILMTAEEIAAVQQAAPWATVIVDHLEAIN
                     HCLETRAYIDRRLGELGARDRVIIPADGELALAS"
BASE COUNT          689 a         1260 c         1444 g          779 t
ORIGIN      
        1 gaccagacgc cggaaaaggg tggcttcaac ctctcgcgct gggtgagccc tgactacgac
       61 aagctgaacg accagcaact cgtcgagttc gacgatgcga agcggatggc gttgatcgag
      121 caggccagcg atatcgtcaa cgacgaagtc gcggagtgcg tcctcttctt cccgaagggg
      181 gcgaccgggt cggcgacgac gctgcataac ttcacaccga acgcatacgg tgacttctgg
      241 caggcgccgt tcgtctggat cgaccagaag tagggcaacg ccagccaacg caccccatcc
      301 gggatcaccg ggtggggtgc gttgtgttgc ctcggacggt caggcctggc tggcggtcga
      361 tgcctcgttc gctcccgggt cccgcaatgg cgttccggcg cggggatcgg aagtcagata
      421 tcgccgcgtc aactcgttga ggaggcactg gccgagttcg aggatctggt cctcgacgcg
      481 gggttgaacc gagagatacg tgagttcgcc gtgcgtcgtc cgcagtccaa gccgcttcat
      541 cagcatcgcc gagaggggcg tgacggaatc gatgacgtcg ttcgcttcgg tgtcgggaaa
      601 catgatcgga agctcggtaa accattgctc gatcgcgtgg tcgtcccgct gctcgagcca
      661 gccatgcaga cgtcgcatat cgtcttccga gaatgccact ccgtcgacct cgatgatcat
      721 gagatacctc gcattccgct gacgtgtgcc tccagtcgac atcacaccgt accagggtcg
      781 agggtcggaa tccggaactg atttcccatt cccggaccat gccggctgga gcgcatcgat
      841 gccgacatcc gtatcaagat tcgcgccatc aaacgcagcg ctggggtggg ctcgattcgc
      901 cacggccggg gaaacgccct gcgttcaggg ctcgtcccgc cgcaacctgc gagcaagctc
      961 ccacgcggcg gcttcgatgc gatccagccg gtcgctcgag cgaccgggcg cctcgttcgg
     1021 gaacgtccgc ttgtcgccgt ttcggatcgg tccatgcggc aagacacaag gtcttgccct
     1081 acagaccccg gctaatcgga gcgccttgcg gggtgggtgt tccggcgaag ggcgctccgt
     1141 tcccgtcgtg cgctccccgt cccggcgacc cacgtggtcg cccgggtcgg tccattcggc
     1201 aagcccagga taaactccgc cgattcgtct tcggggccca gtggcgtgga tggtgccgcg
     1261 ggagcctccc gcggccgatg gtccacgcca gggaggatgc agcttcgctc gaaatgacga
     1321 cctctttatg cgacagccct gggtcttgtg cgccgctgca tagcgggctc gaaagaagca
     1381 cgccgcgccc cgacacggct accgtccgca gtcctggcgg gtcagattcc gtcgcttgcg
     1441 aatcgcgaac gcggtcacga tcccgatcgc tgccaggagg atgagcatgc ggctcgtgat
     1501 atcgtccgtc ttttccgcgg ggatgctccc cgtatttggc agtccactca ggctcacggg
     1561 ggaggcgaga gtgttaaccg cactgccgaa ctgccccgcg ttcgaatcgg tggtgtcatt
     1621 tgaagacgta tcgccgcctt ccgatccggt ggaggatcct cctccgccca atggcgtcgg
     1681 ctgcccgttc gtggatccgc ccccgccccc ggtcggaacc tgagtcggga tcgtctgggg
     1741 actcgccccg acgatggtcg gcgtgagttc gccactgtca ccgttgccgc cagttggcgt
     1801 cggtgtttgt gcgggcggaa tgcccgtcga gaccgggccg cttccgccgg cagtcgttgc
     1861 cgtcggggtg gggagcgtgg cgcccgcggt tccggtggcc tcggctcggg tcgttgggac
     1921 gctcgtcgga agtccgccgg ggggtgtttc gccgggggcg accgtagcgg tgggacccgt
     1981 gccgggtggc gtgttgacgg ggatggtcgt tgcggtcggg accgcggtgg ggccggtcgt
     2041 cggtgtcacc gtggcggctc cgcatgcgat cgcgtccagg ttgttgacgc aggttccgcc
     2101 attgttgccg ctgagcgtgc catcagggca ggtcgtggac gatccccagg tgacactgac
     2161 gaacgtggct ccgtcgaagg tcgatcccgc caggttagcc ccgttcaggg tggtcgcatt
     2221 caggactgcg ccgttgaagt tcgaacagct gagattcgcg ccatcgaagg tcgccgcggt
     2281 gaggttggca ttgctgaaat tggagccgct gacgttggct ccattgaagg tcgctccggt
     2341 caggtcggat ccgctgaaat tcgctcccgc gagatcctgc ccgtcgtaga cacaggcggc
     2401 cagtgccttg cctgcccctg gcgtgcaatc cgtggcggcg gcgttgacgc cggatgcggg
     2461 tggcgttgcc gtcgcggggt gggtgggtgt cgccgtgggt gcttcggatg gtgtcgtggt
     2521 tggtgcgacg gtgccggatg gcgtcgtgat gccggtgggt gtcgtggttg gaccttcgag
     2581 taccggtgtg gcgccgtcga tcggagccga ggtcgtcacg atcccggctg gtgccggtat
     2641 cgcggcggcg aacgccggcc ccgatggcag cacgaacagc gcgaacatga cgagaacgac
     2701 cgcgagatgg cgcggtgcgt ggattcgttt cattccaggt ctttcacgag gctgcctgcg
     2761 aactgcagcc gggggctcgg tcgccaacgg cggccaagtg atggggctcg acgcgcggaa
     2821 gggaagagga gcgaccgcat gtcgcgaggt gagttcgagc ggagacactg aggaggagaa
     2881 tgcggacgcc tgaacagacg tcatacggag aatgattccg ggaccagagg gaggcgccga
     2941 acatcttccg gggaatactt ccaaacatac cagacgatca atgccgttgc ctatagtaca
     3001 agagacctat agggcaaaat ttgctcgaaa atgaatttcg acattggcat gacgaaaatg
     3061 ctccgacgct tcgccacacc gctcgccacg gtggcctctg ggtgctcgca tgggggcatc
     3121 gcgttcgctg gcgagccgcg tcgggcaagg aagtatccga gggaggccgc gcgcgcgggc
     3181 gctgccgtta atgaagcggc gagcagatac tggaggtcga tattgaagga gtggtgggcg
     3241 cggattcgct gcgtattccc gtgattgtgc gggatacttg cggtcgttct gtttgttcgt
     3301 gcctcgatct gacggagatc cgcctgtgaa gatatggctg ctgcgcaacg cgacgctggt
     3361 gctcgactat ggcgagcgga cgattctcgt cgatccgatg ctcgaccccg ccggcgcgcg
     3421 acccgcggtc gcgaacacgc cgaacgatct gcggaatcca ctggtcgagc ttccgcttcc
     3481 gatcgacgag atcctcagcg gtatcgacgc gctgctcgtc acgcatctcc atcaggacca
     3541 cttcgattcg acggcgaagg agcggctggc gaagacattg ccactcatcg gtcagcccga
     3601 ggatgtcgag cgcctgaccg tggacggatt cacgaatctc ctccccgtcg acgacgagcg
     3661 tctctgggaa gggatcgcga tcaggcggac ccgcgcgcag catggcacgg gcgagatcgc
     3721 gaagctgatg gcgccggtat ccggcttcgt gctggaggcc gagggtgaac caacgctcta
     3781 cctcgcgggg gacacgatct ggtacgagcc ggtcgcggcg gtgctcgcga cccatcgacc
     3841 cgacgtgata gtcctgaacg gcagtggcgc tcgcttcaac gtgggcgacc cgatcctgat
     3901 gacggccgag gagattgccg ccgttcaaca ggcggcgcca tgggcgacgg tgatcgttga
     3961 ccacctcgag gcgatcaacc attgcctcga gacgcgcgca tatatcgacc gaagattggg
     4021 ggagcttggc gcgcgtgacc gggtgatcat tccagcggac ggggagcttg ccctcgcctc
     4081 ctgacgcttc accggccgcc ggcgcaacgg tccgtcttgc gtggacgtcg aggctcggct
     4141 aaggtcgcta cggtggattt cgacgggaat gg
//
LOCUS       DASTMD010000185         1905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2338065, whole genome shotgun sequence.
ACCESSION   DASTMD010000185 DASTMD010000000
VERSION     DASTMD010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338065"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VFQ54_02795"
     CDS             complement(<1..545)
                     /locus_tag="VFQ54_02795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0163943.1"
                     /translation="MPTIPTTRTWVRVAYTMFCIGWGANMFAPLLLLYRDRLHLSDTV
                     GSEIFGVYALALIPSLLIGGKLSDMHGRRPILRIAAFASLLASLLLIGGSDREWLLFA
                     GRGMAGIASGLAFSAATAWVKELSFAESPGTGARRAAVAMAGGFGSGGLFGGMIAQWA
                     PAPAHTPYLVHIGMIVVAIGVV"
     gene            complement(633..>1905)
                     /locus_tag="VFQ54_02800"
     CDS             complement(633..>1905)
                     /locus_tag="VFQ54_02800"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEU0163944.1"
                     /translation="DLEEPPIERVLRLTRLRIETAASGSKDARIVFHSLGQDDAQRFR
                     QELLRARQHAIAGEQPATEPAAAGVTPSLDPATARTLDESELIRKLSTRDLILAGATS
                     GRVGPAAALVGILSQFANEYMPRSWWDHVPWRDATSGNAGIIAGLVVTFGVIVWILAI
                     GSTVLTHAGFELRHAGDQLTIQHGLLERRRSTIPLRRIQAVVLLEGLFRQPFGYGELK
                     FESAGQAGGDDGRSGVLFPFLPMREMESLIARITPEYALEIAPAAVRTLPPRAMQRYV
                     VPGLGWTLGLGVLVNVLFWKWLDGIQWWTVAVFALVPIVTYIRWARYRAAGWFVSPVH
                     LMMRWRTITGRVTMITRVQRLQHRSLTANPFQRRARLVTFRASVASGSSGGHFALPQV
                     DRADAEHLLWQLNRRNARAAAAAATEASARK"
BASE COUNT          350 a          599 c          666 g          290 t
ORIGIN      
        1 acaacgccta tggcgacgac gatcatcccg atgtgaacga ggtacggcgt gtgggcgggc
       61 gccggcgccc actgcgcgat cataccccca aagagaccgc cacttccgaa gccacctgcc
      121 atcgcgaccg cggcccggcg cgcaccggta cccggcgact ccgcgaagga gagttccttc
      181 acccaggcgg tcgccgcact gaaggcgagg ccggatgcga ttcctgccat cccgcgtccg
      241 gcaaagagca gccattcacg atcgctaccg ccgatcagga gcagcgatgc aaggagcgac
      301 gcgaaggcgg cgatccggag gatcggtcgt cgcccatgca tgtcggagag ctttcctccg
      361 atcaggagcg atgggatgag ggcgagcgca tagacgccga agatctcgct gccgacggta
      421 tccgagagat ggagacgatc gcggtagagc aggaggagcg gtgcgaacat gttcgcgccc
      481 cagccgatgc agaacatcgt gtaggcaacg cgaacccagg tcctggttgt gggaatcgtc
      541 ggcatggtga ccttgagaat cgatcgggag ttcgaaagga ggcgcggtta cgtttcgtcg
      601 gtcacgaccg atgggcgtgg cgtggctgtg ttctatttgc gggcactcgc ctccgtcgcc
      661 gctgccgcag cggcgcgtgc gttccggcga ttgagttgcc agagcaggtg ctccgcgtcg
      721 gcgcgatcga cctggggaag cgcgaagtgg ccgccggaac tgccggacgc aacggatgcc
      781 cggaaggtga cgagccgtgc cctgcgctgg aatggattcg cggtcagcga gcgatgctga
      841 agtcgctgca cgcgcgtgat catcgtgacg cgaccggtga tcgtccgcca gcgcatcatc
      901 aggtgaacgg gagagacgaa ccagccggcg gcgcggtagc gtgcccagcg gatgtaggtc
      961 acgatcggga ccagggcgaa gacggccacc gtccaccact ggattccgtc gagccatttc
     1021 cagaaaagga cattgacgag gactccaaga ccgagtgtcc agccaagtcc gggcacgaca
     1081 tagcgctgca tcgcgcgcgg cggcagcgtc cgcaccgccg ccggcgcgat ctcgagcgca
     1141 tattccgggg tgatgcgggc gatcagggac tccatctcgc gcatcgggag gaacggaaaa
     1201 aggacaccgg agcggccatc atcgccgcct gcctgacccg cgctttcgaa cttgagctcg
     1261 ccatagccga agggctgccg gaagagcccc tccaacaaca cgacggcctg gatccggcgc
     1321 agcgggatcg tcgagcggcg gcgctccaga agaccatgct ggatcgtcag ttggtcgccg
     1381 gcgtggcgta gttcgaatcc cgcgtgggtg agaaccgtgc tgccgatcgc gaggatccag
     1441 acgatgacgc cgaaggtgac gaccagtccg gcgatgatcc cggcgtttcc cgaggtcgcg
     1501 tcgcgccagg ggacgtgatc ccaccagctc cgcggcatgt attcattcgc gaactgggag
     1561 aggatgccga cgagggcggc cgcgggaccg acccgtcctg atgtcgcgcc ggcgaggatg
     1621 agatcgcggg tcgagagctt gcggatcaac tcgctttcgt ccaacgtacg cgcggtcgcg
     1681 gggtcgagtg acggcgtcac gccagccgcg gcaggttccg tcgctggctg ttcgcccgcg
     1741 atcgcgtgtt gccgcgcacg caggagttcc tgacggaacc gctgggcgtc gtcctgcccc
     1801 aacgagtgga agacgatcct ggcgtccttg ctgccgctcg ccgcggtctc gatccggagc
     1861 cgagtcagcc ggaggacgcg ctcgatcggt ggctcctcca gatcg
//
LOCUS       DASTMD010000186         3316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1625200, whole genome shotgun sequence.
ACCESSION   DASTMD010000186 DASTMD010000000
VERSION     DASTMD010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625200"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2074)
                     /locus_tag="VFQ54_02805"
     CDS             complement(<1..2074)
                     /locus_tag="VFQ54_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU0163945.1"
                     /translation="MLDQSSRLLQGLNNDQQRAVITTEGPVLVVAGPGSGKTRVLTHR
                     IAYLIEEKGITPNQILAVTFTNKAAREMRDRIERLINRETAEGLVMGTFHSLGVRILR
                     QNPGLVSDKLDLRPNFLIYDSGDQAETAKRAITTIGQDPKQIAPRRMLSRISAAKSVL
                     MSPDEMAAETQTYDDEIAARVYKEYERQLRANNAVDFDDLLGLPIRLFDQAPSLLERY
                     QRQYRYIMVDEYQDTNRVQYVMVAALSEHYRNLFVVGDPDQSIYGWRQADIKNILDFE
                     KDYPDANQIHLEINYRSTQRIVQAADRLIRDNEQRIDRKIRTENPEGEPIAVRELSDQ
                     NHEAQFVVSEIRRLTTSGRFKSDDIAIMYRTTAQSRVLEEAFRVTDIPYRIVGGVRFY
                     ERKEVRDVLAVLRLLHNPADRLSLERMIDNLPVGRGFGPKAMDTAVTWAAQNQRPVID
                     AFLAVVPSSFPSEFDDLERPPFSGPGKTGAERIGKTLANLREKAKTSSLAELFDTIVQ
                     QTGFRESFNDGSEEDLQRWANVLELRADLERFDMVDPADALAAYLEQVSLIADVDSME
                     DDDRGQVTLITLHSAKGLEFPVVFITGVEEGLLPISRAVEAEAFDRTPMEEERRLFYV
                     GVTRAEKLLYLTYAASRISYGRFQAGVASRFLQSIPAEAVRSSTRSGMRSSANGRLSS
                     VTSGPLASR"
     gene            2251..2325
                     /locus_tag="VFQ54_02810"
     tRNA            2251..2325
                     /locus_tag="VFQ54_02810"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2283..2285,aa:Met,seq:cat)
     gene            2796..>3316
                     /locus_tag="VFQ54_02815"
     CDS             2796..>3316
                     /locus_tag="VFQ54_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163946.1"
                     /translation="MVVPGSYGSLSAPDVAAQVSNHVTGLTLQCNPPGWPYGQATLYV
                     EKPSGAPIEVDVYIGPSATGPWQAAPSSTVYVTTNSGPYFEAYNFQVAIPSSGRNYIQ
                     TDVEGYESSGNIFGPCPSNDIPTPTPTPTKIPTATPTHTPSPVPTNTPTSTPTDTPTP
                     VPTSTPTPVPTSTT"
BASE COUNT          602 a         1008 c         1067 g          639 t
ORIGIN      
        1 cccgactcgc gagcggacct gacgtgaccg acgagagccg gccattcgcc gagcttcgca
       61 ttcccgaccg cgtcgaactc cgcacggcct cggccgggat gctctggagg aagcgggagg
      121 ccacgcccgc ctggaagcga ccgtaggaaa tgcgactcgc cgcataggtg agatagagca
      181 gcttctcggc gcgggtgacg ccgacataga aaagacggcg ctcttcttcc atcggcgtgc
      241 gatcgaacgc ctctgcttcg acggcacggc tgatcgggag gagaccttcc tcgacgccgg
      301 tgatgaagac cacggggaat tccaggccct tcgccgagtg gagcgtgatc agcgtcacct
      361 ggcctcgatc gtcgtcttcc atcgagtcga catcggcgat cagcgatacc tgctcgagat
      421 aagccgccaa tgcgtcggcg gggtcgacca tgtcgaatcg ctcgagatcg gcccggagct
      481 cgaggacgtt ggcccagcgc tggaggtcct cttccgagcc atcattgaag gattcgcgaa
      541 agcccgtctg ctgcacgatc gtgtcgaaca gttcggccag gctcgaggtc ttcgccttct
      601 cgcggagatt cgccagcgtc ttgccgatcc gctccgcacc agtctttccg gggccactga
      661 atggcggccg ctcgagatcg tcgaattccg acgggaacga cgacgggacg accgcgagga
      721 aggcgtcgat gaccggacgc tggttctggg ctgcccatgt cactgcggta tccatcgcct
      781 taggaccgaa gccgcggccg accgggagat tgtcgatcat ccgctcgagg ctgaggcgat
      841 ccgcgggatt atgcaagagc cggagcacgg cgaggacgtc acgaacctcc ttccgctcgt
      901 agaagcggac gccaccgacg atgcggtagg ggatgtcggt gacgcggaac gcctcctcga
      961 gaacgcggct ctgggcggtc gtgcgataca tgatcgcgat gtcatcgctc ttgaatcgcc
     1021 ccgaggtcgt caagcgtcgg atttcgctga cgacgaactg ggcctcgtga ttctgatccg
     1081 agagttcgcg tacggcgatc ggttcgcctt cgggattctc cgtccggatc ttgcgatcga
     1141 tgcgctgctc gttgtcgcgg atcaggcgat cggccgcctg gacgatccgc tgcgtggacc
     1201 ggtagttgat ctcgaggtgg atctggttgg catccggata gtccttctcg aagtcgagga
     1261 tgttcttgat atccgcctgg cgccagccgt agatcgattg atcgggatcg ccaacgacaa
     1321 agaggttgcg gtagtgctcg gaaagcgcgg cgaccatcac gtactggacg cggttcgtgt
     1381 cctggtactc atcgaccatg atgtagcggt actgacgctg gtagcgctcc aggagcgaag
     1441 gcgcctgatc gaagagccgg atcggcagac cgagcaggtc gtcgaaatcg accgcgttgt
     1501 tggcgcgaag ctggcgctcg tattccttgt agacgcgagc ggcgatctcg tcgtcatagg
     1561 tctgcgtctc cgccgccatc tcgtcgggtg acataagcac gctcttggcg gcggagatcc
     1621 gcgagagcat gcgacggggc gcgatctgct tcggatcctg gccgatcgtc gtgatcgcac
     1681 gcttggcggt ctctgcctgg tcgccgctgt cgtagatcag gaagttgggg cgcaggtcca
     1741 gcttgtcgga aaccagcccc ggattctggc gcaggatgcg gacaccgagc gagtggaatg
     1801 tgcccatcac gagaccctcg gcggtctccc ggttgatcag gcgctcgatg cggtctcgca
     1861 tctcgcgggc ggccttgttc gtgaacgtca cggcgaggat ctggttcggc gtgatgcctt
     1921 tctcctcgat cagataggcg atccggtgcg tcagcacgcg tgtcttcccg gatccggggc
     1981 cggcgacgac cagcacgggt ccttcggtgg tgatcaccgc gcgctgctgg tcgttgttga
     2041 gcccttgaag cagccgggaa gattgatcga gcatggtggg catggtcctt gatcgtaaag
     2101 gagttgggca cagggatggc gaaaggtgat cccggagcgc gacgattgac tctgctgagt
     2161 ttagcactcg cgttcgactt tccccgtgac agaggggtgc gttggttgac gcgccggggt
     2221 gactttggta gagtatccgc cgttgccaat gtcgacgtag ctcagcggca gagtagggga
     2281 ctcataagcc cttggtcggt ggttcgaatc cacccgtcga caccacatga ttcgcggccc
     2341 cgaagtttcg gggctttttg cattttcgag accccgcgag cacgcggttc accggtgtcc
     2401 ccggtggcga tgatgccgta tcgaccggcg tcgtcccgac cgcgatgccg gcgaagatcg
     2461 acgctccgtc gtccacgacc ggcgagatca tgccagagca atcgaaagag gacggccatg
     2521 agcccggacg caatcacgtc cgggctctct tgccgagcgg cggcttccac tcgcgatcgc
     2581 gtctcggggc atgacggcgt ctggacatcg atgcgacaat aaatgcgtga tgtctctctg
     2641 accgggtctt gccccggagc aaccggattt ccgacctccg tttcttctgt gacgtgcgta
     2701 tcgttgccaa tatggggaag gggcttcctg tgacgagagt cgtaggcatt gtgcgacggg
     2761 tgattctact cgcgttgatg agtctatttg cgagcatggt cgttcctgga tcctacgggt
     2821 cgctcagcgc acctgacgtc gcggcgcagg tctcgaacca tgtgaccggc ctgacgctcc
     2881 agtgcaatcc accgggatgg ccgtacggtc aggcgaccct ctacgtcgag aagccaagcg
     2941 gggcaccgat cgaggtcgac gtgtatatcg gcccgtcagc aaccggtccc tggcaggcgg
     3001 cgccaagcag cacggtgtat gtcacgacga attccggacc atatttcgag gcgtacaatt
     3061 tccaggttgc gatcccatcg agcggcagga actacatcca gacggatgtg gaggggtacg
     3121 aatccagcgg gaatatcttc gggccgtgcc cttccaacga tatcccgacg cccacaccca
     3181 cgccgacgaa gatcccaacg gccactccca cgcatacgcc gtcgcccgta ccaacgaaca
     3241 ctccgacgtc aacaccgacg gacaccccga caccggtacc aaccagcacc ccgacaccgg
     3301 taccaaccag taccac
//
LOCUS       DASTMD010000187         4280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2427840, whole genome shotgun sequence.
ACCESSION   DASTMD010000187 DASTMD010000000
VERSION     DASTMD010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427840"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..1465
                     /locus_tag="VFQ54_02820"
     CDS             302..1465
                     /locus_tag="VFQ54_02820"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU0163947.1"
                     /translation="MLLARHQRMSIPASSRSTDRWRDFDFVMFLTMLVLTGFGVVTVW
                     SAVGLPPLLSNNEGTKQALFAAVGVVLLFISSNINYRYLESAAWVLYILGIGALLVVL
                     SPLGYAVDNTGAQNWINLGFTTIQPSEFTKITTIIGLSAFISSRGDAMKEFGNFVLAG
                     LIVALPTGLILVGPDLGQAMVYLSLWASALIVARTRRIFLWGLIIVLPAAIWFAWNYV
                     LQDYQRTRILVSYNPNLDPQGDGYQIIQARISIGAGGLLGSGIRGGTQSTLNLLGVRE
                     SDFIFAHASGMFGFVGMVALFLCLVIFLWRCVKVAEISRDTFGQCIAMCTAGVLFFQS
                     FLNIGMNVGIMPVAGITLPFVSAGVSSLWTFMILTGILQSIRMHHRKLVFERR"
     gene            complement(1462..2292)
                     /gene="rsmI"
                     /locus_tag="VFQ54_02825"
     CDS             complement(1462..2292)
                     /gene="rsmI"
                     /locus_tag="VFQ54_02825"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU0163948.1"
                     /translation="MGTLYVVATPIGNLEEMSPRAIATLKSVNLIACEDTRRSATLLR
                     AFNIETPMISYHKHNLRSREDRLLRALTEGDVALISDAGTPGIADPGLELVEAAHREG
                     IRVVPISGPSAVASAVSVAGFVKGPFLFLGFLTRTGDERRAELARAAAVGYPVVIYES
                     PVRLGETIADLAAVFGGRRAMVARELSKLHEELAHGTLVDLRDRYADAEVKGEIVIVI
                     DGATESVSVEIDVRAVIRGMLEQGMKPSRIAREAASLTDMPGSDVYELVRELQRETPS
                     "
     gene            2504..>4280
                     /gene="dnaX"
                     /locus_tag="VFQ54_02830"
     CDS             2504..>4280
                     /gene="dnaX"
                     /locus_tag="VFQ54_02830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU0163949.1"
                     /translation="MTDGSLFNLSGLEQASPAQQSGDAGSLGQSQSLYRKYRPQTFAE
                     DDLFGQEHVVNTLRNAIALNRIAHAYLFCGPRGTGKTTTARLLAKAVNCLDPDPYKRP
                     CNVCASCVAINKGATTDVIEIDAASNRGIDDIRDLRERVKYAPTQLRTKFYIIDEAHQ
                     ITGAAANAFLKTLEEPPAHTKFVLATTDPEELLQTIVSRCQRFDFRRINLEAMQACIR
                     KVAGEESIAIDDDAVMAIARHATGSLRDALGLLDQLAVYREDDQHDRSRVTLDLVRTV
                     LGVSRNDRVEAIVRALADRDAAAGLQAIGGALDDGEDMRQLGRQLVSYIRMLLLQRAG
                     GSDDADQTARELAERFSLHEIAGLVKLFADIDYKVKHATLSQLPLELAIVEGTLRGQS
                     PAEVPRREAPGLSPAPVESMPARRELREEWTPPEEPERPVERPRTSLRDRVRGAPSAP
                     AERQVRTGGAENPSPAATPISQPSSSTPQPAPVTSTPPPVVAVAAAQVAASNDGPEIR
                     FEMVIELWPKIKSDVKAVNRRIEALLQQIDPVAIVGDKLILVSAYEFHRNRVNADEAR
                     NVIEEVVGRLLKRRLAVSAVTREEAQ"
BASE COUNT          764 a         1461 c         1266 g          789 t
ORIGIN      
        1 aagatcaatc cgaaaaagaa cgcgcccaga agaagcgcat cgagaaaatc atgaatgggg
       61 aacatcgtcg ttctcctgca cgtcgtgtga atggcccacg atcgatcgtc cgtctcgtaa
      121 ttttacgtct tgcagggaca ctttcgggtg gcctcgatac gattcgccca taactgcctg
      181 gttcgtccga acatctgtgg catcataggc gacgacaacg cctcgggtcc cgttcaacca
      241 cgtcttgccg accccggagc gaccagcatc caaccagtgc atcctaccca aaggtctcgc
      301 aatgctcctt gcccgtcacc agcgcatgtc gatcccggcg tccagcagaa gcaccgaccg
      361 ctggcgcgac ttcgacttcg tgatgttcct gactatgctc gtcctgaccg gattcggcgt
      421 cgtcacggtc tggagcgccg tcgggctgcc gccgctcctc tcaaataacg agggcaccaa
      481 gcaggcgctc tttgctgccg ttggcgtcgt cctcctcttc atctcgtcca acatcaacta
      541 ccgctacctc gaatcggccg cctgggtgct ctacatcctg ggcatcggcg ccctgctcgt
      601 cgtgctcagt ccgctcggct acgcggtcga caataccggc gcccagaact ggatcaacct
      661 cggcttcacg acgatccagc cctccgagtt caccaagatc acgacgatca tcggcctctc
      721 ggccttcatc tcgtcgcgcg gcgatgcgat gaaggagttc ggcaacttcg tgctcgccgg
      781 cctgatcgtc gcgctgccga ccggtctcat cctcgtcggc cccgaccttg gtcaggcgat
      841 ggtctacctc tcgttgtggg catcggcgtt gatcgtggcg cgcacgcgac ggatcttcct
      901 gtggggactg atcatcgtcc tcccggccgc gatctggttc gcctggaact acgtgcttca
      961 ggactaccag cgtacgcgca tcctcgtctc gtacaatccg aatctcgatc cccagggcga
     1021 cggctaccag atcatccagg cccggatcag catcggcgcc ggcggcttgc tcggctcggg
     1081 catccgtggc ggcacgcaga gcacgctgaa cctgctcggc gtccgcgaat cggacttcat
     1141 cttcgcccac gccagcggca tgttcggctt cgtcggaatg gtcgcgctct tcctctgtct
     1201 cgtgatcttc ctctggcgct gcgtcaaggt tgccgagatc tcgcgcgaca ccttcggtca
     1261 gtgcatcgcc atgtgtacgg cgggcgtcct cttcttccag tcgttcctga acatcgggat
     1321 gaacgtgggg atcatgcccg tcgcgggaat cacactcccc ttcgtctcgg cgggcgtttc
     1381 ttcgctctgg accttcatga tcctcaccgg gatcctccag agcatccgca tgcaccatcg
     1441 caagctggtc ttcgagcggc gctagctcgg cgtctcccgc tggagttcgc gaaccagctc
     1501 gtagacgtcg gagccgggca tatccgtcag cgacgccgcc tcgcgagcga tgcggctcgg
     1561 tttcatcccc tgctcgagca tcccgcgaat gaccgcgcgc acatcgatct cgacggaaac
     1621 ggattccgtc gccccgtcga tcacgatcac gatctctcct ttgacctccg catccgcgta
     1681 ccgatcacgc agatcgacga gcgtcccgtg cgccagttcc tcatggagct tggagagttc
     1741 ccgcgcgacc atcgcccgcc gtccgccaaa gacggcggcg agatcggcga tcgtctcgcc
     1801 cagccgcacg ggcgattcgt agatgacgac cgggtatccg accgccgccg ccctggcgag
     1861 ttccgcgcgt cgctcatctc cggtccgggt caggaatccc aggaagagaa agggcccctt
     1921 gacgaagccg gcgacgctca ccgccgaagc gaccgcggag ggtcccgata tcggcacgac
     1981 gcggattccc tcgcggtgag cagcctcgac cagctcgaga cccggatcgg cgatgcccgg
     2041 cgtccccgcg tccgagatca gggcgacgtc cccctccgtc agagcacgga gcagccgatc
     2101 ctcacgggat ctgaggttgt gcttgtggta gctgatcatc ggcgtttcga tgttgaaggc
     2161 gcgcagcagc gtcgcgctgc gccgggtgtc ctcgcaggcg atgaggttca ctgacttcag
     2221 cgtcgcgatc gcccggggcg acatctcttc cagattgccg atcggcgtcg ccaccacgta
     2281 gagtgtcccc atgagttccc ttcagaaatg acgcccaact cgagtcgagg acttcaccgt
     2341 cgattgagga acgtttgttc gcacggctat acttcgagac acagaatcga cgccggcgtc
     2401 aacgacgcgt accacggcct gatgacggtc cgaacgtcac ttctccccgc tgccaacgta
     2461 tctacacaca ttatgacgca ttactggaga atggcttgcg ctcatgaccg acggatcgct
     2521 cttcaacttg tccgggctgg agcaagcgag ccccgctcaa caatccggtg atgccggatc
     2581 gctgggacaa tcccagtcgc tctaccggaa gtatcgaccg cagacctttg ccgaggatga
     2641 cctcttcggg caggaacatg tggtcaatac gctgcgtaac gcgatcgcac tcaaccggat
     2701 cgcccacgcg tatctcttct gcggtccccg cggcacgggc aagacgacca cggcgcgcct
     2761 gctcgccaag gcagtgaact gcctcgatcc ggatccgtac aagcggccct gcaatgtctg
     2821 cgcatcgtgc gtcgccatca acaagggcgc aacgacggat gtcatcgaga tcgacgcggc
     2881 gagcaatcgc ggcatcgatg atattcgcga tctgcgtgag cgcgtgaaat acgcgccgac
     2941 ccagctccgc acgaagttct acatcatcga cgaagcccac cagatcactg gcgccgcggc
     3001 gaatgcgttc ctgaagacgc tcgaggaacc tcctgcccac acgaagttcg tgctcgcgac
     3061 gacggatccc gaggaactcc tgcagacgat cgtctcccgc tgccagcgtt tcgacttccg
     3121 tcgcatcaat ctcgaggcga tgcaggcctg catccgcaag gtcgccggcg aggagtcgat
     3181 cgcgatcgac gacgacgccg tgatggcaat cgcacgtcac gccaccggca gtcttcgcga
     3241 cgcgctcggt cttctcgatc agctcgcggt ctaccgcgag gacgatcagc acgatcgatc
     3301 gcgcgtcacc ctcgatctcg tgcgaaccgt gctcggcgtc agccgcaacg atcgtgtcga
     3361 agcgatcgtc cgtgcgctcg ccgatcgcga cgccgccgcg ggcctccagg cgatcggcgg
     3421 cgcgctcgac gacggcgagg acatgcggca gctcgggcga cagctcgtga gctacatccg
     3481 gatgctcctc ctccagcggg ctggcggctc cgacgatgcg gatcagacgg cgcgcgaact
     3541 ggccgagcga ttctcgctcc atgagatcgc gggactcgtc aagctcttcg ccgacatcga
     3601 ctacaaggtg aagcacgcga cgttgagtca acttccgctg gaacttgcga tcgtcgaggg
     3661 aacgctccgc ggtcagagtc cagcggaagt tccacgccgg gaggcgcctg gtttgtcccc
     3721 ggcaccggtg gagtcaatgc cagcccggcg cgagctccgc gaggagtgga caccgccgga
     3781 ggaacccgag cggcccgttg agcgcccccg gacgtccctg cgggaccgcg tgcgtggcgc
     3841 tccgtccgcc cccgctgaac gccaggttcg cacgggcgga gcggaaaacc cgtcgcctgc
     3901 ggccacgccg atttcacagc cgtcgtcctc gacgccacaa ccagcgccgg tcacctccac
     3961 gccgccgccg gtggtcgccg tggccgccgc acaggttgcg gcgagcaatg atggtcccga
     4021 gatcaggttc gagatggtga tcgagctctg gccgaagatc aagagcgatg tgaaggcggt
     4081 caaccggcgg atcgaggcac ttctccagca gatcgatcct gtcgcgatcg tcggggacaa
     4141 gttgatcctc gtctcggcct acgagttcca ccggaatcgc gtcaacgccg acgaagctcg
     4201 caatgtcatc gaagaggttg tcgggagatt gctcaagcgg cggctggcgg tctcggcggt
     4261 tacccgtgaa gaagcccaga
//
LOCUS       DASTMD010000188         1778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2695277, whole genome shotgun sequence.
ACCESSION   DASTMD010000188 DASTMD010000000
VERSION     DASTMD010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2695277"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFQ54_02835"
     CDS             <1..636
                     /locus_tag="VFQ54_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163950.1"
                     /translation="GAFGNAVILDGEILVFGRTADVIEEVIAQSDGTTASMLDTVGVV
                     ETLNAFSPEMVSAIGVSSKAFQTSYPTMTPEQVKEVKSHLAESDDAVGKMPAIEVGAF
                     GVLAGAVLASGDSEGTPEATPVDASSAIAQTRLFLGSASKAKQAAKVVDWRWNHLDSL
                     LIEVPYNQIMTLESAGASDVVDTVAAIDFGGSTAAGNWSRLILTGDVLPFS"
     gene            complement(718..>1778)
                     /locus_tag="VFQ54_02840"
     CDS             complement(718..>1778)
                     /locus_tag="VFQ54_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163951.1"
                     /translation="ETESESSSVSRWSWRAEPTPEESETAANSAGTVPDDEPDADSDA
                     SVAATALESSESPEQAPSSEASPAEPVTSLTPEAADEEGESLSSWAARWNADADEEDE
                     QVAAEAEPAAPEASAAEPEASSTDSEANEREPETPSEPESEPVADTPTDVAPQAELES
                     EPQAEPESKSEPQPEPESEPQATDEPTPAPEEPDLLAGMTAIPAGSSSNEYSWKSTPD
                     PESEPEPEVGLAPASDAAAAKASSDAVLRAESLLDELRDLLPSLAAATTTEDETPPAP
                     GIDTNAVADELSAALPESDRFSDLRETLETARANPRDIDTMLSIVGRAGDLIDLLDTR
                     DALESAISTAIARLRNDS"
BASE COUNT          259 a          541 c          628 g          350 t
ORIGIN      
        1 ggtgcgttcg gcaatgcggt gatcctggat ggcgaaatcc tcgtctttgg ccggacggcc
       61 gacgtgatcg aggaagtgat cgcgcagtca gacggaacga cggcctcgat gctcgacacc
      121 gtcggcgtcg tggagaccct caacgccttc tcgccggaga tggtctcggc gatcggcgtt
      181 tcctcgaagg cctttcagac gtcctatccc acgatgacac cggaacaggt gaaagaggtg
      241 aagagtcatc tcgccgagtc ggacgacgcc gtgggcaaga tgcccgcaat cgaggtcgga
      301 gcgtttggcg tgcttgccgg ggctgtcctc gcgagcggcg acagtgaggg cacgccggaa
      361 gcgacgcccg tcgacgcatc cagcgcgatc gcccagaccc ggctcttcct tggctcggcg
      421 tcgaaggcga agcaggcagc gaaagtcgtc gactggcgat ggaatcatct cgactcgttg
      481 ctgatcgagg tgccctacaa ccagatcatg accctggagt cggcgggtgc ctcggatgtc
      541 gtcgacaccg tcgccgcgat cgactttggc ggaagcaccg cggccgggaa ctggtcgcgg
      601 ttgatcctga ccggggacgt actgccgttc tcctgatcct tgcgtgcaca tccatgcgaa
      661 aaccccggcc agatcgcttc tggccggggt tttcgcattg tctcgcaacc gatcgcgtca
      721 gctgtcgttg cgtagtcggg cgatagccgt cgagatcgcg gactcgagtg cgtccctggt
      781 gtcgagaaga tcgatgagat cgcccgccct gccgacgatc gaaagcatcg tgtcgatgtc
      841 gcgcgggttg gcgcgcgcgg tctcgagcgt ttcgcgaaga tcggagaagc gatccgattc
      901 cgggagtgcg gccgagagtt cgtcggcgac ggcattcgta tcgatgccgg gagccggcgg
      961 cgtctcgtcc tcggtggtgg tggccgccgc aagcgacggc aggaggtcgc gcagttcgtc
     1021 gagcagggat tctgcccgga ggacggcatc actgctcgcc ttcgccgcag cggcatcaga
     1081 cgcgggggcc agcccaactt ccggttccgg ttccgactcg ggatcgggcg tcgatttcca
     1141 gctgtattcg ttggaggatg atccggccgg gatggcagtc attccggcaa ggagatccgg
     1201 ttcttccgga gcgggcgttg gctcgtctgt cgcctgcggt tcagattcgg gctccggctg
     1261 cggttcggac ttggactcag gttccgcttg cggctcggat tcgagctccg cttgtggtgc
     1321 gacatcggtc ggggtgtcgg cgaccggctc gctttcgggt tcgctcggtg tttcgggctc
     1381 gcgttcattt gcctcggagt cagtggacga ggcttcgggc tcggcggcgg acgcctcggg
     1441 agcggcgggt tccgcctcgg ccgcgacctg ctcgtcctct tcgtctgcgt cggcattcca
     1501 gcgcgccgcc caggaagaaa gcgattcgcc ttcctcatcg gcagcttcag gggtgagcga
     1561 tgtcaccggc tctgctggcg atgcctcgga ggatggtgcc tgttccgggg actcgctgga
     1621 ttccagggcc gtggcagcga cggacgcgtc cgaatcggcg tctggctcgt catccgggac
     1681 ggtgcccgcg gagttggcgg cggtctcgga ttcttccggg gtcggctccg ctcgccacga
     1741 ccagcgcgag acggatgacg actcggactc cgtctcgg
//
LOCUS       DASTMD010000189         1849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_17162, whole genome shotgun sequence.
ACCESSION   DASTMD010000189 DASTMD010000000
VERSION     DASTMD010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17162"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1094)
                     /locus_tag="VFQ54_02845"
     CDS             complement(90..1094)
                     /locus_tag="VFQ54_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0163952.1"
                     /translation="MVETGSGDRLRVAIVGAGGWGNQHARAFSARSDIDLCGIYSRSS
                     DRAVERAATYKTRPYTDIDEMLRTEKPDFVSVCLPNDAHFAPTLRVIEANIPLLVEKP
                     LVFDLLEAEQLLEEAERRGLFFAINFNHRYAGPVARARVAIEEGKLGEISFATWRFGG
                     EGSTTHHPHANLIETQCHGIDMLEQLVGPITSVAAEMTDKFAPGYSTLAVALGFANGA
                     VGTLLGSYDSSYAYPQTHLLEINGTLGRILVEDTVKRYTYSRTGDATREVWEAGYFDD
                     RGRDFHQAFDNHLEALLGAFRRGDPPPVPARDGQRVLQISYRIIESFETRRIVDVPHS
                     "
     gene            1293..1466
                     /locus_tag="VFQ54_02850"
     CDS             1293..1466
                     /locus_tag="VFQ54_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163953.1"
                     /translation="MKWQAYASVIVLLYVVSTLLIVQRADPDDRPRRGATFARLSLPV
                     LVAWLAVTSTLLF"
     gene            complement(1482..>1849)
                     /locus_tag="VFQ54_02855"
     CDS             complement(1482..>1849)
                     /locus_tag="VFQ54_02855"
                     /inference="COORDINATES: protein motif:HMM:NF040123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6518 family protein"
                     /protein_id="HEU0163954.1"
                     /translation="IVLGLSLDSYNDPIAWAIGGVTAGPLLAASGYLWRNGSDRWRIV
                     STALLAATFVSEGIYIVNTLGYEIGYAFIGVGATFAVAMAGAWRNLAFAMVLAALLSV
                     ALWAAYRYGYTRAHDAIAR"
BASE COUNT          327 a          557 c          625 g          340 t
ORIGIN      
        1 ctgccaacga tcatatgcgg cttcgccgca tgggccacac ggggtggccc gctaagagat
       61 cacggtggta attcggccgt gacgaatctc tagctgtgcg gaacgtcgac gatgcggcgg
      121 gtctcgaagg actcgatgat cctgtaggag atctggagga cccgttggcc gtcccgcgcc
      181 ggaacgggcg gcggatcccc acgccggaaa gcaccgagca aggcctcgag atggttgtcg
      241 aatgcctggt ggaaatcgcg gccacggtcg tcgaagtagc ctgcctccca gacctcgcgg
      301 gtcgcatcgc cagtgcgcga gtaggtgtat cgcttcaccg tgtcctcgac gaggatccgc
      361 ccgagggtgc cgttgatctc gaggagatgc gtctgcgggt aggcgtagga cgagtcgtag
      421 ctgccgagga gtgtcccgac ggcgccgttc gcgaagccga gcgcaacggc gagcgtgctg
      481 tacccggggg cgaacttgtc cgtcatctcg gcggcgaccg aggtgatcgg tccaacgagc
      541 tgctcgagca tgtcgatccc atggcattgc gtttcgatca ggttcgcgtg gggatgatgc
      601 gtcgtcgatc cctcgccgcc gaatcgccac gttgcgaagc tgatctcgcc gagcttccct
      661 tcttcgatcg cgacgcgcgc ccgcgcgacc ggcccggcgt agcggtggtt gaagttgatc
      721 gcgaagaaga gaccgcgccg ttccgcctcc tcgagcaatt gttcagcttc caggaggtcg
      781 aagacgagtg gtttttcgac gagcagcgga atgtttgcct cgatcacgcg gagcgtcggg
      841 gcaaagtgcg cgtcgtttgg caggcagacc gagacgaaat cgggcttttc cgtccgcagc
      901 atctcgtcga tatcggtgta ggggcgcgtc ttgtaggtgg cagcgcgttc cacggcccgg
      961 tccgatgagc gcgagtagat gccgcagagg tcgatatcgc tgcgtgcgct gaacgcccgg
     1021 gcatgctggt taccccagcc gccggcaccg acgatcgcga cgcgcaggcg gtctcccgat
     1081 ccagtttcaa ccatagatcc acccatcccg cgtcgaacga cgcgattcag ttgttgtcag
     1141 aagcaatgtt tgcgaagact aacgccggaa accgccgggc gtcaatgcgg gcgccggtat
     1201 ggagaccgtt tcatcgccat ttcatgagag agatcgatat tcgaggtagg ttggaggcag
     1261 tgatcgaccg catcgatcag gagagtcgcc ccgtgaagtg gcaagcgtat gcctcggtaa
     1321 tcgtgcttct ctatgtcgtc tcgacgttgt tgatcgtgca acgcgccgat ccggatgaca
     1381 ggccacgccg tggcgcgacg ttcgcgcgcc tcagcctccc ggtcctggtc gcgtggctcg
     1441 cggttacctc gaccctgctt ttctgaggac gaggacgcgg cctaccttgc gatcgcgtca
     1501 tgggcgcgtg tgtagccgta acggtaggca gcccagaggg cgacactcag cagggccgcg
     1561 agcaccattg cgaatgcgag gtttcgccag gcgccggcca tcgcgactgc gaaggtcgcg
     1621 ccgacgccga tgaaggcgta gccgatctcg tatccgagcg tgttcacgat gtagatgccc
     1681 tcggagacga aggtcgccgc gagcaacgcc gtcgagacga tccgccagcg atcggatccg
     1741 tttcgccaga gatagccgct cgcggcgagc aacggcccgg ccgtgacgcc gccgatcgcc
     1801 caggcgatgg ggtcgttgta gctgtcgagc gagagaccga ggacgatcc
//
LOCUS       DASTMD010000190         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_554227, whole genome shotgun sequence.
ACCESSION   DASTMD010000190 DASTMD010000000
VERSION     DASTMD010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554227"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..381)
                     /locus_tag="VFQ54_02860"
     CDS             complement(67..381)
                     /locus_tag="VFQ54_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163955.1"
                     /translation="MGYPEITEDQREMVVEMLANAIRKDAHLRARADRPLPDGTPLRA
                     NLMRRRAEPSQRYIDGMRDLVRVLFVDGQTLATACLEDAYRRAMGISAPVANDGDAPQ
                     AD"
     gene            533..1318
                     /locus_tag="VFQ54_02865"
     CDS             533..1318
                     /locus_tag="VFQ54_02865"
                     /inference="COORDINATES: protein motif:HMM:NF004203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0163956.1"
                     /translation="MTNDGQFAGKVALVTGGGSGIGRAAAIELGRQGASVAVLARQQH
                     RIDEAVESVIAAGGQAFGISADVKSEDALLDAVRQVEEKWGRLDHVVANAGANGVFAP
                     LEELTLDEWHHTMDTNLTSTFLTVKSSLRLLKERGGSIVVVSSVNGSRNFNYPGAVAY
                     STSKAGQLVFAKMVAIELAQMKIRINVVCPGAITTNIDEATFKRNTDSVRWPVEFPKG
                     HSPLRGGPAEASEVANVIAFLLSDAASNVSGTEMWVDGGDTLL"
BASE COUNT          284 a          506 c          515 g          248 t
ORIGIN      
        1 cgaggcatga accccgcgct gccaaccccg gcagcaccgg acgcgccggc ggttcgggat
       61 agcgcgtcag tcagcctgcg gggcgtcgcc gtcgttcgcg accggagcgg agatgcccat
      121 cgcccgccga tacgcatcct cgaggcaggc ggtcgccaac gtctggccgt cgacgaagag
      181 cacgcggacg agatcgcgca tgccatcgat atagcgttgc gacggctccg cccgccgtcg
      241 catcaggttc gcgcgcagtg gggtgccatc cggcagcggt cgatccgcgc gggcacggag
      301 atgcgcatcc ttgcggatcg cattggcgag catctccacc accatttcac gctgatcctc
      361 ggtgatctcg ggatatccca tgcgtctcac tctttcgata atcgcttcac cgcgtatcgt
      421 gctgctgaag atcatagcca ccaaactcat cccgcgagga gtctttcctt cgcgccgccc
      481 agatgtgaga cttgagcgca cggccaatcg cattcgcaaa aaggatcgtt acgtgaccaa
      541 tgatggacag tttgcaggca aggtagcgct cgtcaccggc ggtggttccg gaatcggacg
      601 agcggccgcg atcgagctcg gtcgccaggg agcgtcagtc gcggtccttg cccgccagca
      661 gcaccggatc gacgaggcgg tcgaatccgt gatcgccgcg ggcggtcagg cgttcgggat
      721 cagcgccgac gtcaagagcg aggacgcgct gctcgatgcc gtccgccagg tcgaggagaa
      781 gtggggacgg ctcgatcacg tcgtcgccaa cgccggcgcc aacggggtct tcgcaccgct
      841 cgaggagctg acgctcgacg agtggcacca cacgatggat accaacctga cgagcacctt
      901 cctcacagtg aagtcaagcc tgcgcctgct gaaggagcgc ggcggctcga tcgtggtcgt
      961 ctcctcggtg aacggatcgc gcaacttcaa ctacccgggc gcggtcgcct actcgacgag
     1021 caaggcggga cagctggtct tcgcgaagat ggtcgcgatc gagctcgcgc agatgaagat
     1081 ccggatcaac gtcgtctgcc cgggcgcgat cacgacgaac atcgacgagg cgaccttcaa
     1141 gcgaaacacc gattcggtgc gctggccggt cgagttcccg aagggccatt ccccgctccg
     1201 cggcggcccg gcggaagcgt cggaggtggc gaacgtgatc gccttcctgc tctcggatgc
     1261 ggcgagcaac gtcagcggca ccgagatgtg ggtcgacggc ggcgacacgc tgctgtaacc
     1321 gaaccctacc ctcaaactcg tagcgggcca ccccgtgtgg cccatcgggc gcagcccgat
     1381 atcaagccga gtgagactga cgtggactcg gaactgggcg gccaccttga ggatccggtg
     1441 ccatccggat cctttcggcg atcgcatagc caccgacgag gggtgagcgc ggaccgacga
     1501 agcgtttcga gccgacccct tcgcccgcgt agcgggggag gagctggatg tgg
//
LOCUS       DASTMD010000191         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1981158, whole genome shotgun sequence.
ACCESSION   DASTMD010000191 DASTMD010000000
VERSION     DASTMD010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981158"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFQ54_02870"
     CDS             <1..265
                     /locus_tag="VFQ54_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163957.1"
                     /translation="VGVIGAVVLALPERLGERMQRRRDTISIAHSRGSDGLLPEGPRG
                     ETPIAVDPNWVGLEPNGVRTLIMTKDPDEYTRVGVPQGQDRDE"
     gene            746..1054
                     /gene="nuoK"
                     /locus_tag="VFQ54_02875"
     CDS             746..1054
                     /gene="nuoK"
                     /locus_tag="VFQ54_02875"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU0163958.1"
                     /translation="MGHLTVNHFLFLSAALFIIGMMGMLTRRNVLIIFMCVELMVTAV
                     NISLAAFAYETNTLDGQVFVLFTLAVAAAEAAVGLAIILALSRRGKSVDVDEIRTLRD
                     "
     gene            1163..>2193
                     /locus_tag="VFQ54_02880"
     CDS             1163..>2193
                     /locus_tag="VFQ54_02880"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU0163959.1"
                     /translation="MRYPRHHEWSQQIEGVLMENLLFLIPLLPLLAFLVNGLIGRFVI
                     RANAHWVSAPAVFISWALSVGVLIQIHHDNNPIHQRLFTWIPAGDFNVAASLYADQLT
                     AVMLVVVTTVGLLVHVYSIGYMHGDDGYYRFFAYLPLFVFSMLMLVLADNLLVLFVFW
                     EAVGLCSYLLIGYYFRRRSAVNAAKKAFIVNRIGDVGFGIGIIYTFWTFKTINFYGAN
                     GIFADAANRTALGIAGGTLTAIGILLFIGAMGKSAQFPLHVWLPDAMEGPTPVSALIH
                     AATMVTAGIYMVAWTHVIYMQSETAMLVVALIGAFTAFMAATIAITQNDIKRVVAYST
                     LSQLGYMAIA"
BASE COUNT          371 a          725 c          665 g          432 t
ORIGIN      
        1 cgtcggcgtg atcggcgccg tcgtcctcgc gctgccggaa cggctcggcg agcggatgca
       61 gcgtcgtcgc gacacgatca gcatcgccca ctcccgtggc tcggatggac tgctgccgga
      121 gggaccgcgt ggggagacgc cgatcgcggt ggatccgaac tgggtcggtc tcgagcccaa
      181 cggcgtgcgc acgctgatca tgacgaagga tccggatgag tacacgcggg tcggcgtccc
      241 gcaggggcag gaccgcgatg aataacgcgc aaaagcgacc tcttctatcc ccgggaactg
      301 agtggagcgg catcgccgtc cccagtacgt catcccgagc ggagtgcagc ggcatcgccg
      361 gcaggcgagc cgcgaagcgc agtcgaggga tcagctggca agcaacgtgt cccccggtcg
      421 gaggggagcc cggcggcggt attcggcaga acattctgca tggtgagtgg cccctcgact
      481 gcgctcgatc gctttcccga cgatcgggaa tggaccgctc cactcgggat gacgtaaaag
      541 gggatgtggg aaacgccggg gtttgtaggg cgggaattca tctctcgctg caacgcgacg
      601 tgacgcccca cgatccttcg cggcaacgat cgccgcaaac gcggacgtcc ctagggcagg
      661 acttctatgt cctgccagat gccaacattg cgatcgaccc gccatcgcgg aagacacccg
      721 gcaagaaggc aaggcaggta ccggcatggg ccatctgacc gtcaaccatt tcctcttcct
      781 tagcgcggcg ctcttcatca tcgggatgat gggcatgctg acgcgccgga acgtgctcat
      841 catcttcatg tgcgtcgagc tcatggtgac ggccgtcaat atctcgctcg ctgccttcgc
      901 gtacgagacg aatacgctcg acggccaggt cttcgtgctc ttcacgcttg ctgtggcggc
      961 ggcggaagcc gcggtcgggc tcgcgatcat cctggcgctc agtcgccgcg gaaaatcggt
     1021 cgatgtcgac gagattcgga cgctccgtga ctagtctgca catccgtgcg tcggactccg
     1081 tagatgcgac ccttgtggtc gccttgccgc gaagcggcgc atcgaccgta ggtcgacctt
     1141 gtcacggata cagttcgctg gagtgcggta tccccgccac cacgagtggt cgcaacagat
     1201 tgagggcgtg ctgatggaga acctgctctt cctgattcca ttgctgccgt tgctcgcgtt
     1261 cttggtgaac ggcctcatcg gccgcttcgt catacgcgcc aacgcgcact gggtatcagc
     1321 accagccgtc ttcatctcct gggcgctctc ggtcggtgtg ctgatccaga tccaccacga
     1381 taacaacccg atccaccagc gtctcttcac ctggattccg gccggcgatt tcaacgtcgc
     1441 cgcctcgctc tacgccgacc agctcactgc cgtgatgctc gtcgttgtga cgaccgttgg
     1501 cctgctcgtc cacgtctact cgatcggcta catgcacggc gacgacggct actaccgttt
     1561 cttcgcctat ctgccgctct tcgtcttctc gatgctgatg ctggtgctgg cggacaatct
     1621 gctcgtgctc ttcgtcttct gggaagcggt cggtctctgc tcctacctgc tgatcggcta
     1681 ctacttccgg cgccgctcgg ccgtcaatgc cgcgaagaag gcgttcatcg tcaaccgcat
     1741 cggcgacgtc ggtttcggga tcggcatcat ctacaccttc tggaccttca agacgatcaa
     1801 cttctatggc gccaacggca tcttcgctga cgccgccaac cgcaccgcgc tcggcatcgc
     1861 cggcggcacg ctgaccgcga tcgggatcct gctcttcatc ggcgcgatgg gcaagagcgc
     1921 ccagttcccg ctccacgtct ggttgccgga tgcgatggaa ggcccgacgc cggtttccgc
     1981 cctcatccac gcggcaacga tggtcaccgc tggcatctac atggtggcgt ggacccacgt
     2041 catctacatg caatccgaga ccgcgatgct ggtcgtcgcg ctgatcggtg ccttcaccgc
     2101 cttcatggcg gcgacgatcg cgatcaccca gaacgacatc aagcgcgtcg tcgcctactc
     2161 gaccctgagc cagctcggct acatggcgat cgc
//
LOCUS       DASTMD010000192         1946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1714244, whole genome shotgun sequence.
ACCESSION   DASTMD010000192 DASTMD010000000
VERSION     DASTMD010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714244"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VFQ54_02885"
     CDS             complement(<1..408)
                     /locus_tag="VFQ54_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163960.1"
                     /translation="MTSLDWYREALHESSHARRTRIAQWFRGWPFLKLHLSVFAFAIV
                     VLFALNVARTSGGLWIGTVALAWLAVIVIHAAVGGIIWALDLLRQDPDRLEAEDLPWT
                     AINLRPLGGGGGGRGEPQDIDYRMARESAPAVSD"
     gene            complement(452..961)
                     /locus_tag="VFQ54_02890"
     CDS             complement(452..961)
                     /locus_tag="VFQ54_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163961.1"
                     /translation="MSSASLGSTEQDRSNVVNDLVATYRELNLRIRPLSESQLAADRG
                     GSSIKSLVGDLRDEEVAFAQLLKEEVTGITVDEALLADAPRTQVESGEDSTAMIISQF
                     GTARATTLSLLKSLDDAGWDKPTAEGKTIYQLATATATRDKSYLDRILSGQQGATSPQ
                     VTGSSFAAS"
     gene            1424..1816
                     /locus_tag="VFQ54_02895"
     CDS             1424..1816
                     /locus_tag="VFQ54_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163962.1"
                     /translation="MNHPAPVSTPRDRIRVHTPMHCPICAKELGSVIVRDLGGVTADI
                     VWQLHAGECPEHGWFQTEFVSRPPREIFAVTRPFGAARRIVVNGHEYFSFSTAWNDLT
                     PQEKRQRVNPLEEKYWQTRPVQNVATAS"
BASE COUNT          371 a          654 c          577 g          344 t
ORIGIN      
        1 gtcagaaacc gccggcgcgg attcccgagc catccggtag tcgatatcct ggggctcgcc
       61 tcgacctccg ccgcccccac cgagcgggcg caggttaatc gcggtccacg gcagatcctc
      121 cgcctcgagc cggtccggat cctgcctgag caaatcgagc gcccagatga tgccgccaac
      181 ggcggcgtgg atcacgatca ccgccagcca ggccagcgcc accgtcccga tccagagacc
      241 gccactcgtc cgcgcgacgt tgagcgcgaa gagcacaacg atcgcgaagg cgaagacgga
      301 gagatggagc ttcaggaacg gccagccccg aaaccactgc gcgattcgcg tccggcgggc
      361 atgcgaactc tcgtgcaatg cttcccgata ccagtccagt gaagtcatgg aacgattcat
      421 cggaacctcg aggctatccg ggagtttctc gttacgaggc ggcgaaggag ctgcctgtca
      481 cctggggcga tgtcgcccct tgctgtcctg ataatatcct gtcgaggtag gacttgtcgc
      541 gagtggccgt cgcggtggcc agctgataga tggttttccc ctccgccgtc ggcttgtccc
      601 atcccgcatc gtcaagtgac ttgagcagcg acagggtcgt ggcgcgagcg gttccgaatt
      661 gggaaatgat catcgccgtc gagtcctccc cggattcgac ctgcgtccgc ggcgcatccg
      721 caagaagcgc ctcatcgacc gttatcccgg tcacctcctc cttcagcaac tgggcaaagg
      781 caacctcttc atcgcgcaga tcccccacga gcgacttgat ggacgaccca cccctgtcgg
      841 cggcgagctg ggattcagaa agcggacgga tgcgaaggtt gagttcgcga taggtcgcga
      901 cgagatcgtt gacgacgttc gagcgatcct gctctgtaga accaagcgac gctgacgaca
      961 tgatgcctga cctccgttga agcattccgt gactcggacg gcgttggccg ttcccggcgt
     1021 agctcggata tggatgtcgc cccacccgat cgagcggaga tagcacaaca gtgcgcagga
     1081 accacgccag cctcattcct gcccggatgg gcaatagttg ttcatcgcac acaaccaggc
     1141 tgcagtagaa acggtgataa ctggtcatgg gacgatccgg cgtcctctcc ggccaaacgg
     1201 cccgcctggt cgggaagaaa ttcatatgaa tgcttcgatg ggaactgcta gactagctga
     1261 aactgatctg cggcctgcac gatcaggcgg gtaattccgg tcctcggcgt catcccgcgc
     1321 attcctccgg tcgctggcgt gcctctcctc cacgttcccc catccgccac gttcgtcctc
     1381 cttcatatcc aggcgctccc gacagcgcag agagggtaga ccgatgaacc accccgcccc
     1441 cgtctcgacc ccacgcgatc gcatccgggt tcatacgccg atgcactgcc cgatctgcgc
     1501 caaggaactc ggcagcgtca tcgtgcgtga cctcggtggc gtcaccgccg acatcgtctg
     1561 gcaactgcat gccggcgagt gcccggaaca tgggtggttc cagacagagt tcgtctcgcg
     1621 gccaccgcgc gagatcttcg cggtcacgcg gccgttcggc gcggcgcggc ggatcgtggt
     1681 caatggccac gagtacttct ccttctcgac cgcctggaac gacctcaccc cccaggagaa
     1741 gcggcagcgc gtgaatccgc tggaggaaaa gtactggcag acgcgacccg tccagaatgt
     1801 ggcgaccgcg agctagtcac atcccgcgaa gtcgaacgat gcccctggta gcggaacccg
     1861 ttgccagggg catttcccgc cgccgaccat tgacccgact ggccgcttcg ggcccttgcg
     1921 acccagcgtc actatccgga cagcat
//
LOCUS       DASTMD010000193         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2874809, whole genome shotgun sequence.
ACCESSION   DASTMD010000193 DASTMD010000000
VERSION     DASTMD010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2874809"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VFQ54_02900"
     CDS             <1..947
                     /locus_tag="VFQ54_02900"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0163963.1"
                     /translation="DAFGYRQVIPAREPMSVDTIFDLASLTKVVATLPAILHLVQTRV
                     FSLDDPLKRHLPIPATHPLGDATIEQLLTHSAGLPPRTSLNQYGHARRDMLRGLLDSP
                     LDAPPGANVIYSNRGFLILGLLIESVSSLSLDRYVSKHVWTPLGMHETWFSPPEAVFS
                     RVAPTEFRERLGSCQRGTVHDENAALLGGVAGHAGVFSTARDLARFCAVIMASVRQAS
                     PPILDPELVRLSLRDHTPTLNESRGLGWEIETGEDAEELIFSHLGFTGTSIHLNPARD
                     MFVLLLANRVHPTRDDPDSIREIRREVARLCWRLADRH"
     gene            999..1277
                     /locus_tag="VFQ54_02905"
     CDS             999..1277
                     /locus_tag="VFQ54_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163964.1"
                     /translation="MAVTLPNHLERQLRELIEQGTFEDPEDVLEHAFALLSEESRNTR
                     LKAALAESAQDVAEGRTAPLTPELITRLTAEAAEMVRKGVRPDPDVAF"
     gene            1700..1981
                     /locus_tag="VFQ54_02910"
     CDS             1700..1981
                     /locus_tag="VFQ54_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163965.1"
                     /translation="MAVKISPENQRRIQEHIDRGVFKDADDFVTRTLDLTDEIERRRA
                     RLAAIIDESEADIQAGRVYEMTPELWAQIRDESEEMHRRGVPLDPDVYP"
     gene            complement(2263..>2716)
                     /locus_tag="VFQ54_02915"
     CDS             complement(2263..>2716)
                     /locus_tag="VFQ54_02915"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163966.1"
                     /translation="YAGHSAGGHLAVWASRRHLLPPGSPGSRGSALSVSGVVALAPVL
                     DLGEAFRLDLGGGAVAALLGGPPESFPDRYRVADPVRLRGAGPKVTIIHGDEDRHVPI
                     GMSRTYRDVVGAELIELPGVEHFALIDPESDAWQKVAEAFGSTPAIES"
BASE COUNT          563 a          875 c          777 g          501 t
ORIGIN      
        1 acgacgcgtt cggttaccgt caggtcatcc cggcaaggga accgatgagc gtggacacga
       61 tcttcgatct tgcctcgctc acgaaagtcg tcgccactct cccagcgatc ctccatctcg
      121 tacaaacgag agtcttctcg ctcgacgatc ccttgaagcg gcatctgccg attccagcaa
      181 cgcacccgct cggcgacgcg acgatcgagc agcttctgac ccatagtgcg ggtctccctc
      241 cgaggacgag cctgaaccaa tatgggcatg ctcgccggga tatgttgcgc ggactgctcg
      301 attcacccct ggatgcccca cccggcgcca acgtgatcta cagcaatcgc ggctttctca
      361 tcctcggcct gttgatcgag tcagtcagca gcctctcgct ggatcgctac gtcagcaagc
      421 acgtctggac tcccctcggg atgcacgaga cctggttcag cccgcctgaa gcggtcttca
      481 gccgcgtcgc gccaacggaa ttccgcgaac gtctgggaag ttgccagcgc ggaaccgtcc
      541 acgatgaaaa cgcggccttg ctcggcggag tcgctgggca cgcgggcgtc ttctcgaccg
      601 ctcgcgacct cgcccggttt tgcgccgtga tcatggcttc cgtgcggcag gcgtcgcctc
      661 cgatcctcga tccggagctg gtgcgtctct cgcttcgcga tcacacgccg acgctgaacg
      721 agtctcgcgg tctgggatgg gagatagaga ccggcgaaga cgccgaggaa ctcatcttca
      781 gccatctcgg attcacgggc accagcatcc atctcaatcc agctcgggac atgttcgtcc
      841 tcttgctggc gaaccgggtt cacccgactc gcgacgatcc cgattcgatc cgcgagattc
      901 gccgggaggt ggcgcgcctc tgctggcgat tggctgaccg gcattgacga tttccaccat
      961 cgtatccgct atgcttgaaa caatgaaagg acaagcccat ggccgtgaca ctccccaacc
     1021 accttgaacg tcaactccgg gaattgatcg agcaggggac cttcgaagac ccagaggatg
     1081 ttctggagca cgcgtttgcg ctgttgagtg aagaatcccg aaacactcgc ctcaaggcag
     1141 cactcgccga gtcggcacaa gacgtcgccg agggacggac cgctccgctg acgccggaac
     1201 tcatcacgcg tctcactgct gaggcagcgg aaatggttcg aaagggagtg cgcccggatc
     1261 ccgatgtcgc attctgaggt tcgactcgag ctatcatccc gcgcccagcg cgatatccgg
     1321 aacactatgc gtcgggtctc ctgctgtgag ggacaggtgc cgccgcaacg tatcagggcc
     1381 gcattctcga gaagcttgaa ctcattcgcc tcaatccgct tatcgggagg acttgccgcg
     1441 acctgggaga cgaacttaga agctttccgg tcgagagaca catcatcgtg tacgagattc
     1501 gcgacaatat cgtgttcgtt cgaaacatca tccatagtcg tatggatatc cccggaaacc
     1561 tcaacaagaa acccagccaa taagtgataa ccacgagact cgccgggggg tggcgagcct
     1621 ctgctggctg ccggccgatc gcaattgaca atctcgctcc ccgcttccgc tatgcttgtg
     1681 gcagcgaaag gatgcctcca tggccgtcaa gatttccccg gaaaaccagc ggcgtatcca
     1741 ggaacacatc gaccgtggcg tgttcaagga cgccgacgat ttcgtaacgc gcacgctcga
     1801 cctgacggac gagatcgaac gccgacgtgc gcgtcttgcc gcgatcatcg acgagtccga
     1861 ggctgatatc caggcgggtc gcgtctatga aatgaccccc gaattgtggg cccagatccg
     1921 cgacgagtcg gaagaaatgc accgtcgcgg cgtaccgttg gaccccgatg tctatccctg
     1981 agaatcgagc caggtattcg gcccgcgcca ttcgcgatct ggggcgcatc cgccgatacg
     2041 ggctggagaa ttggggacca acgacaaccg aagactacgt tacgaggatt gcggacactg
     2101 tttcacttct ggtggagaat ccgcagctgg gagttctccg cgaggatatt ctccagaacc
     2161 tccggagcat tccgtcggaa caacacgtag tcttttactc gatcattcgc ggagagatcg
     2221 tcgtccgcgc aatcgttcat cagcgtatga atctcgacgc gatcaacttt cgatagcagg
     2281 ggttgatcca aacgcctccg cgactttctg ccacgcgtct gactcagggt cgatgagcgc
     2341 aaagtgctcg acgccgggca gttcgatcaa ctccgccccg accacatccc gataggtcct
     2401 cgacattccg atcgggacgt gacggtcctc atcgccatga ataatcgtca ccttcggccc
     2461 agccccacgg agccgcacgg gatcggcgac gcgataacga tcggggaacg actccggggg
     2521 accaccaagc aacgccgcaa ccgccccgcc accgagatcg agccggaacg cctcgcccag
     2581 gtcaagaacc ggggcgagtg cgaccactcc agaaaccgat agcgcgctgc ctcgcgaccc
     2641 gggcgatccc ggtggcagca gatgtcgccg cgatgcccag accgcgagat ggccaccggc
     2701 ggaatgacca gcgtag
//
LOCUS       DASTMD010000194         3278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_286147, whole genome shotgun sequence.
ACCESSION   DASTMD010000194 DASTMD010000000
VERSION     DASTMD010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286147"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1609
                     /locus_tag="VFQ54_02920"
     CDS             320..1609
                     /locus_tag="VFQ54_02920"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0163967.1"
                     /translation="MSSAFRDYRTRTFASLSLYNYRLFFIGQCISQSGTWMQTIAQGL
                     LVLQITGSGTALGIVTALQTLPVLIFGAWGGVFADRMNKRSVLYVTNSIFGIGSLATG
                     ILTVSGDIQLWMVYIVGLVFGFTKVFDNPARQTFIREMVGNDYLQNAVSLNSTQMNLA
                     RVIGPSIAGLTAATIGLGACFIFDAASYAIVFSMLHRMRESELHPAKKLARAKGQLVE
                     GFRYAAETPMLRTILLMMAIIGTFTFEFSVLLPLFAEFTFHRGAGGYAALTSAMGVGA
                     VVGGLYTASRKVRGTTTLVLSAALFGVSVAAASLAPTLIFAVAAFVVVGFFSINFTSL
                     ANSTLQMHTIPAMQGRVMALWSIAFLGTTPIGGPIMGFVGQHAGARWGLLLGGIAAII
                     AAGAGLISVSRSGANVIPATVMQAVPSSGTAKASGAD"
     gene            1736..2455
                     /locus_tag="VFQ54_02925"
     CDS             1736..2455
                     /locus_tag="VFQ54_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0163968.1"
                     /translation="MHHSPDTRTRNGSGVPATILVVEDDPNVRYVIVGGLRLAGYEIE
                     IETTGRAALERLRTNGDQIGLVVLDVMLPDLDGFEVCRRLRRDRLETPIIFLTARDTL
                     VDRVEGLTIGGDDYLAKPFGIEELVVRISVILRRVGRHASMMPLTSGPLTLDDDAHVV
                     RLRDTEVSLSPTEYKLLRYLMLNKGRALSRGQILDHVWDYGFAGESTVVESFISTLRK
                     RVDPEGTLIQTIRGVGYRLEG"
     gene            2458..>3278
                     /locus_tag="VFQ54_02930"
     CDS             2458..>3278
                     /locus_tag="VFQ54_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0163969.1"
                     /translation="MTLLTRLMAIAGIALVISAAGGALLSQSIRTTELAPLDDQLRAA
                     LPVALTLPVGETASPLAAIGNLYLARVAPDGTRDVIARPVTVPDREPRLPGSLSNSSA
                     PDPVTEGSVSGSGSWRVVYVASSPGDVILAVPTIGRHDAPRVVTASLFVGRLVVLIIV
                     ALVGWWLIRLALRPISEATRVATAVASGDRTRRLAVGRTRSEGNQMARAFNQMLDEQY
                     AGEERLRRFIADASHELRTPVAAIGGFADLYRLGGISQDELDDAMRRIGQESARMR"
BASE COUNT          579 a         1061 c         1009 g          629 t
ORIGIN      
        1 attgcccgca accgattcca attcgaacga accgatgcat aatgccgcca gaccagcggt
       61 gacagcaatt cgacggtggg gcgcgggcct cacgacatga catgacataa ggtttcgccg
      121 gtagaccaat cggaaacacg gagaagggga ggggaagaaa aaacatgaac atgccttccg
      181 gccctgactc ggcaataaca ggacagtcat caatggagcg ccaggaaaag tcgtctcgcg
      241 gcagattcaa gacatggttg gatcacaacg gagcaacggg gatgtacaac acatcacccc
      301 cgatcaaagg agtcgcgcga tgagcagcgc attccgcgac taccgcacac gtacgtttgc
      361 ctcgctgtcg ctctataact accggctttt ctttattggg caatgtattt cgcaatccgg
      421 cacctggatg cagacgatcg cccagggcct cctcgttctc cagatcacag ggtccggtac
      481 cgcactcggg atcgtgaccg cattacagac gctaccggtc ttgatctttg gcgcgtgggg
      541 cggcgtcttc gcggatcgca tgaacaagcg cagcgttctc tacgtgacga attcgatctt
      601 cggcatcgga agtctggcaa ccggtattct gacggtcagt ggcgacattc aactatggat
      661 ggtctatatc gttggtctcg tattcgggtt caccaaggtc tttgacaatc cggcccgcca
      721 gacattcatc cgcgagatgg tcgggaacga ctacctgcag aacgcggtct ccctcaactc
      781 gacgcagatg aacctcgccc gcgtcatcgg tccgtcgatc gccggtctca cggcggcgac
      841 gatcgggctc ggcgcctgct tcatcttcga cgcggcatcc tatgccatcg tcttctcgat
      901 gctgcatcgg atgcgcgaaa gcgaactcca cccggcgaag aaactcgcac gggcaaaggg
      961 gcaactggtg gagggattca ggtacgcggc ggaaacgccg atgctgcgga caatcctgct
     1021 gatgatggcg atcatcggta cgttcacctt cgagttctcc gtactcctgc cgctcttcgc
     1081 cgagttcacg ttccaccggg gcgctggcgg atacgcggcg ctgacctccg caatgggtgt
     1141 cggcgccgtc gtcgggggtc tctacacggc gagccggaag gtgcggggaa cgacgacgct
     1201 cgtgctctcg gcggcactct tcggtgtttc cgttgccgcg gcatcgcttg cgccgacgtt
     1261 gatcttcgcg gtggccgcct tcgtcgtggt cggcttcttc tcgatcaact tcacgtcgct
     1321 cgccaactcg acgctccaga tgcacacgat tcctgcgatg caaggtcgtg tgatggcgct
     1381 ctggtcgatc gcgttcctcg gcacgacgcc gatcggtggt ccgatcatgg gctttgtcgg
     1441 tcagcacgcc ggcgcgcgct ggggcctgct cctcggcggt atcgcggcga tcattgccgc
     1501 gggcgccggt ctgatctcgg tcagccgcag cggagccaac gtcatccccg cgacagtcat
     1561 gcaggcggtg ccgagcagcg gcaccgccaa ggccagcggg gccgactgat aacccgatgg
     1621 cgaaggcgtc gcacgtgcat gtggatcgag atgacccggc caactatgat ggccgggtca
     1681 tctccaccgg gccgcgcacc gggcgccgtc cgagcatctc gtcgaatagt cagccatgca
     1741 tcattctcca gacacccgca ccaggaatgg atccggggtt cccgccacaa ttctcgtcgt
     1801 ggaggacgat cccaacgttc gctacgtcat cgtcggcggt ctccgtctcg ccgggtacga
     1861 gatcgagatc gagacgaccg ggcgcgccgc actcgagcgt ctgagaacga atggcgatca
     1921 gatcggcctc gtcgtactcg acgtgatgct cccggatctc gacggcttcg aggtctgccg
     1981 aagactccgg cgcgatcggc tcgagacgcc gatcatcttc ctcactgcgc gggatacgct
     2041 cgtcgaccgg gtcgaggggc tgacgatcgg cggtgacgac tatctcgcca agccgttcgg
     2101 aatcgaggag ctggtcgtcc gcatctccgt catccttcgt cgggtcggca gacatgccag
     2161 catgatgcct cttacgtccg gtcccctcac actcgacgac gacgcccacg tcgttcgtct
     2221 tcgcgatacc gaggtgtcgc tctcgccaac cgaatacaag ctgctgcgct acctcatgct
     2281 gaacaagggc cgggcgctga gccgcggcca gattctcgat catgtctggg attacggatt
     2341 tgccggcgaa tcgacggtcg tcgaatcctt catctcgacg ctgcgcaaga gggtcgatcc
     2401 cgagggaacg ctgatccaga ccatccgcgg cgtcggctac cggctcgaag gctgaccgtg
     2461 acgctcctta cccgcctgat ggcgatcgcc ggcatcgcgc tcgtcatcag cgcggcgggc
     2521 ggcgcgctgc tctcgcaatc gatccgtacc accgagctgg cgccgctgga cgaccagctt
     2581 cgcgccgcac taccggtcgc gctgaccctg ccggtcgggg agacggcgtc accgttggcc
     2641 gcgatcggca atctctacct cgcccgtgtc gctcccgatg gcactcgtga cgtcatcgcg
     2701 cgcccggtca cggtgccaga ccgcgagcca aggctgcctg gatcattgtc gaattcatcg
     2761 gccccggatc ccgtcacgga aggctccgtc tccggttcgg gatcgtggcg cgtcgtctac
     2821 gtggcgtcgt caccgggcga cgtgatcctg gctgttccca cgatcggacg acatgacgcc
     2881 cctcgcgtcg tgaccgcttc gctcttcgtc ggccgcctcg tcgtcctcat catcgttgcg
     2941 ctcgtgggat ggtggctgat tcgcctcgct ctccgcccga tcagcgaggc gacgcgagtg
     3001 gcgaccgccg ttgcgtctgg tgaccgcacc cgtcggctcg cggtggggag aacccgatcc
     3061 gagggcaacc agatggcgcg ggcgttcaat cagatgctcg acgagcagta tgccggtgaa
     3121 gagcgattgc ggcgcttcat cgccgacgca tcgcacgaac tccgcacgcc ggtggcggca
     3181 atcggaggct tcgccgatct ctatcgtctc ggtggaatct cgcaggatga actcgacgat
     3241 gcgatgcgca ggatcggaca ggaatccgcg cgcatgcg
//
LOCUS       DASTMD010000195         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2428444, whole genome shotgun sequence.
ACCESSION   DASTMD010000195 DASTMD010000000
VERSION     DASTMD010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2428444"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VFQ54_02935"
     CDS             complement(<1..514)
                     /locus_tag="VFQ54_02935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU0163970.1"
                     /translation="MSSSAPIAIPHRTREHIRVGVVFPQTEIGDAPADIREFATTVED
                     VGFVHLLAYDHVVGGQLDHYPKLQGRYTSETPFHEVFVLFGYLAAITTKLELVSGVII
                     LPQRQTTLVAKQAAEIDLLSGGRLRLGIGIGWNDIEYEALNEDFRNRGRRIEEQISVL
                     RALWTEDVLTF"
     gene            739..>1517
                     /locus_tag="VFQ54_02940"
     CDS             739..>1517
                     /locus_tag="VFQ54_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163971.1"
                     /translation="MPDADRRDHQHVIPFTRRRFLAGLAGAASGLLIPLSAERAAAQD
                     QVIEPMDPTATETPATTDVPTTDTSSGSTQIESTEPTPSDSQGADLSSADVADTTKAL
                     YFAAPGHNLTTPFLEAWTALGGEAVLGQPLSEGRFLGATGTIQQAFETLTLEYNPALD
                     APLDMQATHLDSAFVTSTAPSASRTKVNGCGGADASCQFFPDSGHTVSETFADFWTAH
                     SGLQMLGVPLSESFKKGSVTSQVFERAVLDQDTKGNVTIRK"
BASE COUNT          280 a          490 c          470 g          277 t
ORIGIN      
        1 tgaaggtgag aacgtcttcc gtccacaggg cgcggaggac ggagatctgc tcctcgatcc
       61 gccgcccgcg gttacgaaag tcctcgttca gtgcctcgta ctcgatgtcg ttccagccga
      121 ttccgatccc gagtcgcagc cggcccccgc tcagcaggtc gatctcggct gcctgcttgg
      181 cgacgagcgt cgtctggcgc tgcgggagaa tgatcacgcc ggaaacgagt tccaacttcg
      241 tcgtgatcgc cgcgagatag ccgaagagca cgaagacttc atggaagggt gtctcgctgg
      301 tgtagcggcc ctgcagcttc gggtagtgat cgagttgacc gccaaccacg tggtcgtagg
      361 cgaggaggtg gacgaatccg acgtcctcga ccgtcgtggc gaactcgcgg atatcggccg
      421 gtgcgtcccc gatttcagtc tgcgggaaga cgactcccac ccggatatgt tctcgcgtcc
      481 ggtgtggaat tgcgatcggt gccgaagaac tcatatgctg gaaacctccg tgatgcctgc
      541 ttcgttaaag cacacgagcc cacgcgcaac agcggtgaga acgtggctcc ctgaaaggtg
      601 atacgacgca acgagtgatg cgtctcggcc cgctgttcct gcttcgtacc gctctccccg
      661 ccgggatttt ttccggagat ggacggggat caatcctacc gttgggatag tgactgtcaa
      721 cagggaaggt gagtctccat gcctgacgcc gatcgccggg atcaccaaca cgtcatccca
      781 ttcacccgac gccgttttct tgccggtctg gccggagctg ccagcggact cctgatcccg
      841 ctttcggccg agcgtgccgc cgcacaggat caggtcatcg agccgatgga tccaaccgcc
      901 acggagacgc cggccacgac cgacgttccc accaccgata cgtcatccgg atcgacgcag
      961 atcgaatcga ccgagcccac tccgtcggac agccaggggg cagacctctc ctcggccgat
     1021 gtcgccgata ccacgaaggc cctctacttc gcggctcccg gacacaatct gaccacgccg
     1081 ttcctcgaag cgtggactgc cctcggcggc gaggccgtcc tcggccagcc gctctcggag
     1141 ggacggttcc tcggtgcgac ggggacgatt cagcaggcgt tcgagacgct gacgctcgag
     1201 tacaatccgg cgctcgacgc gccgctggac atgcaggcga cgcatctgga ctccgccttc
     1261 gtgacatcga cggcgccgtc cgcatcacgg acgaaggtca acggctgtgg cggcgccgat
     1321 gccagctgcc agttctttcc ggattccggt cacacggtga gcgaaacgtt cgccgacttc
     1381 tggacggccc acagcggcct ccagatgctg ggcgtgccgc tctcggaatc gttcaagaag
     1441 ggaagcgtca cgagtcaggt cttcgagcgg gcggtcctcg atcaggatac gaaggggaat
     1501 gtcacgatcc gcaagat
//
LOCUS       DASTMD010000196         1561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_375367, whole genome shotgun sequence.
ACCESSION   DASTMD010000196 DASTMD010000000
VERSION     DASTMD010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375367"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..707)
                     /locus_tag="VFQ54_02945"
     CDS             complement(180..707)
                     /locus_tag="VFQ54_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0163972.1"
                     /translation="MFDHIIAVLSGSTLDDTALSIAADEALRHNAVLVLLRTIDPGRE
                     STLERNLRGQTGRQGEAGSGLLEVTARSEAVAYLEAISRRHHLYSRVELVVRKDMVSS
                     AIIDEATCRQRSLVVMPFGAEEGHIPGMRQGTLPLLLALLGCPLMFVGSNTNPPRQYA
                     AEVFERNDAYAGIPF"
     gene            1097..>1561
                     /locus_tag="VFQ54_02950"
     CDS             1097..>1561
                     /locus_tag="VFQ54_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163973.1"
                     /translation="MILTVNAGSSSLKVGLFHGEGSDRSEISSTIERLDSRGGRWTFV
                     SGHEQGEGSRVHSTHDDALVELLEWLDARGYRDRIEAAGHRVVHGWITHVEPTVVDEA
                     LLGDLERLIPMDPGHLPQAIACMRILTRSLPELPQIACFDTAFHHTMPRVARM"
BASE COUNT          288 a          463 c          499 g          311 t
ORIGIN      
        1 gtcccggcgg gctcactctc ctacccgggt tacccgggtg ggacgagcga gcgcaggatt
       61 ccaaggcgaa gtcgggagct gccgacaggg ttcgtgatgc gcggggcttt atgccccgcc
      121 gcaaagcgga ccctgaagca acacgccgta tcccggggtg gttcctgtcc cggtcgccgc
      181 tagaacggga tcccggcgta ggcgtcgttc cgctcaaaca cctcagccgc gtattggcgc
      241 gggggattcg tgttcgaacc aacgaacatg agcggacatc ccagcagagc gagcagaagc
      301 ggaagcgttc cttggcgcat acccggaatg tggccctctt cggcgccaaa cggcattacc
      361 accagtgaac gctgccggca cgtcgcctcg tcaatgatcg cgctggagac catgtccttt
      421 ctcacgacga gttcgacccg gctatagaga tgatggcggc gggagatcgc ttcgagatac
      481 gccaccgcct cgcttcgcgc ggtgacttcg agaagaccgg aaccggcttc tccctgcctc
      541 cccgtctgcc cgcggaggtt tcgctcgaga gtggattcac gcccgggatc tatcgtgcgc
      601 agcaggacaa gaaccgcgtt gtgacgtagc gcctcgtcag ccgcgatcga gagtgcggtg
      661 tcatcgagtg tcgagccgct gagtacggcg atgatgtgat cgaacatcac ggtgcctccc
      721 ttcgatggag cgatcgtcca gaactccatg acctgtcggt catgcgatca gcttctcatt
      781 caggtcatcg caatcgactc tcaaattcct cgctaccatt tcaaatgaat gtcagttcgg
      841 gatgccgggc cgggcacgaa cttgtaacgc ggatgccggt cagtttgacc tttcttcaat
      901 gaatgcccgg tacgatggat tcgtgaggat tcgtcatcca cgtgccagct gcgtcgcacg
      961 gactccggtg tcgagacgcg tgacacggag gatcccccgt gggggtggct ggaccgttcg
     1021 atcagtctga tggcaacctc tgtgcttcag atcggatacg acggtggcgg atcgcgatga
     1081 gttcccggga gtgcccatga tactgaccgt aaatgccgga tcgtcgagcc tgaaggtcgg
     1141 actctttcac ggtgaaggga gcgatcgttc cgagatttcg tcaaccatcg agcgcctcga
     1201 cagtcgaggg gggcgctgga cattcgtctc gggccacgag cagggggaag gttcgagggt
     1261 tcactcgacg cacgatgacg cgctggtgga gctgctcgag tggctggatg cccgaggtta
     1321 tcgggaccgg atcgaggccg ccgggcatcg tgtggttcac ggatggatca cccacgtcga
     1381 gccgacagtg gtcgatgagg cgttgcttgg cgatctggag cgtctgattc cgatggatcc
     1441 ggggcatctg ccccaagcca tcgcgtgcat gcggatcctg acacgatcgt tgccggagct
     1501 tccccagatc gcctgcttcg atacggcctt tcaccatacg atgccgcgcg tcgccagaat
     1561 g
//
LOCUS       DASTMD010000197         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1178208, whole genome shotgun sequence.
ACCESSION   DASTMD010000197 DASTMD010000000
VERSION     DASTMD010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178208"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFQ54_02955"
     CDS             <1..626
                     /locus_tag="VFQ54_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0163974.1"
                     /translation="LRDQRDYIQSRDYLNEWGAYHPGVTLLSEMTLGLLWMGEIARPI
                     ARVARAFNMRVIYWDIAQFPELEQEIGMEFVSWDELFRQSDVVSTQLALNEKTEGIIG
                     AKEFALMKPTALFTNSARGRLVDQEALIAALQSGAIGGAALDVYYDEPLPTDSPLHTL
                     HEDGSHRVILTPHSAAQGPWTWVHDSQDLWFNIRACLDGQPLKYLVE"
     gene            1056..1349
                     /locus_tag="VFQ54_02960"
     CDS             1056..1349
                     /locus_tag="VFQ54_02960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEU0163975.1"
                     /translation="MIVLLAKYYVKEGNMDAVLAAFAEAVPLVLANEPECSRYEISRA
                     VEDPNLILINERYADMAAVDFHRATPHFKEIVQGRIWPLLDNRVPEFFETVIA"
     gene            1360..2397
                     /locus_tag="VFQ54_02965"
     CDS             1360..2397
                     /locus_tag="VFQ54_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0163976.1"
                     /translation="MPDPRPRPQFGYCLPIFANPSAGLFRTPNLERVHAPETLALGRY
                     AEEIGFDSLWVADHLMLGRDEAVLEGWTILSALAGSTSRARIGMIHQAHYFRSPSLAA
                     KMIGTLDQVAEGRLILFYDFGRQQREHLAYHLPYPDDVDVRVRETIDGLKLILDLWSA
                     NSPLSTTRGPYGVTNAVANHGPVQQPHPPIWFGEVEEGLLDACAEFGQGWNTTPCGID
                     ELNRRFGLLRNDCDRAGRDYDEIEKSVELQVLLTTEEGSRAKLRDLLAKAPDQDAIDP
                     ELLAYANGESAAPPASFTDMTLIGSPAEVEVQLRAYVDAGADHFLLWFLDAPERTGMD
                     LFAREVAPAFA"
     gene            2519..>2936
                     /locus_tag="VFQ54_02970"
     CDS             2519..>2936
                     /locus_tag="VFQ54_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163977.1"
                     /translation="MTFTRMNRRTILQASAIASGVGGLGLLAASTMAQGAPPSGGQGG
                     PNGTPGAGGGQGGPGGTPPAGGGGQGGPGGSQTLEDFIGVTTNGTPLENLYPVFATGV
                     SMEPVKKAADAFLATLDETRLAATQLEVDDDIFRQWT"
BASE COUNT          529 a         1026 c          878 g          503 t
ORIGIN      
        1 gcttgcgcga ccagcgcgac tacatacaat cgcgtgacta cctcaacgag tggggcgcct
       61 atcatcccgg cgtcacgcta ctgagcgaga tgacgcttgg attgctgtgg atgggcgaga
      121 tcgcccgccc gatcgcccgt gtcgcccgcg ccttcaacat gcgggtgatc tactgggaca
      181 tcgcccagtt cccggaactc gaacaggaga tcgggatgga attcgtctcc tgggatgaac
      241 tcttccggca gtcggatgtc gtctcgaccc agctcgcact gaacgagaag acggaaggaa
      301 tcatcggggc gaaggagttc gcgctgatga agccgaccgc gctcttcacg aactccgccc
      361 gcggcaggct cgtcgaccag gaggcgctga tcgcggcgct ccagtccggc gcgatcggcg
      421 gcgcggcgct ggacgtctat tacgacgaac cgcttcccac cgacagcccg ctccacacac
      481 tgcatgaaga tggcagccac cgggtaatcc tgacaccgca ctccgcggca cagggtccgt
      541 ggacgtgggt ccacgactcg caggatctct ggttcaacat ccgtgcctgt ctcgacggcc
      601 aaccgctgaa atacctcgtc gagtgataac aacccgagac gcccagtgat gacggtcacc
      661 aacccagact cacacacctc cgtcatcctt cgctccgctc aggatgacga ggggatgtgc
      721 tggcacgcgg tcgttcgtag gggaggactg agtcgcgaag accgggcgag ggacggaatg
      781 tcctccctcc cgctgtcccg gcgcatgttc ccgccccacg cacgaccgcg gcgtcccatg
      841 ctattcggct gcgctccgat acaactaccg ggatgaactc cgccgatcca tgcggcgttc
      901 gtacgtaggg tgggaccgcc gttgccggac cgcagtccgg acggcctgtc ccgcctctcg
      961 cgtcccgccg acgttgccct cttgctcctt cgcacgcagc atggcacgct ggtgcaacgt
     1021 catcagttcg atagattccc ggaaaggatc ccctcgtgat cgtgctcctc gcgaagtatt
     1081 acgtcaagga aggcaacatg gacgcggtcc tcgccgcctt cgccgaggcc gtgccgctcg
     1141 tcctcgccaa cgagcccgaa tgctcgcgct acgagatcag ccgcgccgtc gaggacccga
     1201 acctgatcct catcaacgag cgttacgccg acatggccgc cgtcgacttc caccgcgcga
     1261 cgccgcattt caaggagatc gtccagggcc ggatctggcc gctgctcgac aaccgggtcc
     1321 ccgagttctt cgagaccgtc atcgcctgag gtatgcacga tgcctgatcc gcgaccacgc
     1381 ccgcagttcg gatactgcct gccgatcttc gccaacccct ccgccgggct cttccggacg
     1441 ccgaatctcg aacgcgtcca cgccccggag acgctcgccc tcgggcgata tgcggaggag
     1501 atcggcttcg attcgctctg ggtcgcggat catctcatgc tcggcaggga tgaggccgtg
     1561 ctcgaaggct ggacgatcct ctccgcgctc gccggatcga ccagccgcgc gaggatcggc
     1621 atgatccatc aggcgcacta tttccgctcc ccctcgctcg ccgcgaagat gatcggcacg
     1681 ctcgatcagg tcgcggaggg ccgcctcatc ctcttctacg acttcggtcg ccagcaacgc
     1741 gaacatctcg cctatcacct gccgtacccg gatgatgtcg acgtccgggt acgggagacc
     1801 atcgatggcc tcaagctgat cctcgatctc tggtcggcga actcgccgct ctccacgaca
     1861 cgtggcccct acggcgtgac gaacgccgtc gccaaccacg gaccggtcca gcagccacat
     1921 ccgccgatct ggtttggcga ggtggaggaa ggactgctcg atgcctgcgc cgaattcggt
     1981 cagggctgga acacgacgcc ctgcggtatc gacgaactga accggcgatt cggtctcctc
     2041 cggaacgact gcgatcgcgc gggtcgcgat tacgacgaga tcgagaagag cgtcgaactc
     2101 caggtcctgc tgacgacgga ggagggttcg cgcgcaaagc tccgcgatct cctcgcgaaa
     2161 gcaccggatc aggatgcgat cgatccggaa ctcctcgcct acgccaacgg cgagtccgcc
     2221 gcaccgccgg catccttcac cgacatgacg ctgatcgggt cgccggcgga agtcgaagtg
     2281 caactccgcg cttacgtcga tgctggcgcc gatcacttcc tgctctggtt cctcgacgcc
     2341 ccggagcgca cggggatgga ccttttcgcg cgcgaggtcg ctcccgcctt cgcgtgacgg
     2401 caccacccac aacagatccc atacacggac catatgctgg ctcaggcatc ctccttgcag
     2461 gctgcaatcc ccgaccggtg tggattgccg aactcctcac gtaccaaggg gaatgacctt
     2521 gaccttcact cgtatgaatc gccgcaccat cctgcaggcg agtgccattg cctcgggcgt
     2581 tggcggactc ggcctcctcg ccgcatcgac catggcccag ggagcgccac cttccggagg
     2641 tcagggcggg ccgaacggga cgccgggagc cggcggtggc cagggtggcc ccgggggaac
     2701 accgccagct ggaggcggcg gccagggtgg ccctggtggt agccagaccc tcgaggattt
     2761 catcggcgtc acgaccaacg ggactccact cgagaacctc tatccagtct tcgcgaccgg
     2821 ggtctccatg gagccggtca agaaggccgc cgatgccttc cttgccaccc tcgatgaaac
     2881 ccggctcgcc gcaacccagc tcgaagtcga tgacgatatc ttccgccagt ggacca
//
LOCUS       DASTMD010000198         1528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1981545, whole genome shotgun sequence.
ACCESSION   DASTMD010000198 DASTMD010000000
VERSION     DASTMD010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981545"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VFQ54_02975"
     CDS             complement(<1..961)
                     /locus_tag="VFQ54_02975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0163978.1"
                     /translation="MRKRRTFVNRRTLKAPHTPSTMRIAERRREAGHDQDAIYIGPFR
                     ITFGLVFFLILVAAAAVRFAGIDWGSSYYLHPDERYVTEVTAAMKWPGSLSAYFDSAT
                     STLNPFNDPKYTSYVYGDFPLLVSKFLGTIVGDTVYGNAHTPGRWLSAISDIGTVAMA
                     GWASRTLFGRIAGLFTATLLAFTALMIQAAHYYTTDSMTGFFVTATMAFVVYASKKRS
                     WAGYMVAGLMFGLAVACKPNAAASVGFFAIPLLEQIRLRGWRSILPQSRARRAEDRFP
                     ILIASLVAGFIALWTFRFAQPYAFLGPHWWSFKLDPRWTDNLSY"
     gene            complement(954..>1528)
                     /locus_tag="VFQ54_02980"
     CDS             complement(954..>1528)
                     /locus_tag="VFQ54_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0163979.1"
                     /translation="SHIVCSEVLEHLPDDGKAVAEMFRVLKPGGVLTVTVPSAKFPFG
                     WDPGNWVLDRISGRQLRGERPWSGIWYGHQRLYTKSNLGALIAGHGFTVEETRGLTHY
                     SPPFAHLVMYGIGKPLIQKGLVPAKLKKQADRRMDDAPPPSGLTGIAMRVLEWIDAPN
                     DASDLDDRKDSFVAIAIRARKPITPSTRDA"
BASE COUNT          283 a          456 c          522 g          267 t
ORIGIN      
        1 agtagctgag gttgtccgtc caccgggggt cgagcttgaa tgaccaccag tggggaccga
       61 ggaaggcgta gggctgggcg aagcgaaagg tccagagcgc gatgaatccc gccaccaggc
      121 tcgcgatcag gatcggaaaa cgatcttcgg cgcgccgcgc gcgggattgc ggcaggatcg
      181 atcgccagcc gcggaggcga atctgctcca ggagcggaat cgcgaagaag ccgaccgacg
      241 ccgcggcgtt cggcttgcag gcaacggcga gtccgaacat cagcccggcg accatgtacc
      301 cggcccagct tcgcttcttc gatgcgtaga cgacgaaggc catcgttgcg gtaacgaaga
      361 accccgtcat cgagtccgtc gtgtagtagt gggcggcctg gatcatcagg gcggtgaacg
      421 cgagcagggt cgccgtgaag agaccggcga tgcggccgaa gagcgtccgg ctggcccagc
      481 ccgccattgc cacggtgccg atatcggaga tcgccgagag ccagcgcccc ggggtatgag
      541 cgttcccgta cacggtatcg ccgacgatcg tgccgaggaa tttgctcacc aggagcggga
      601 agtcaccgta gacgtagctc gtgtatttcg ggtcgttgaa gggattcagg gtcgatgtcg
      661 cgctgtcgaa atacgcgctc aggcttcccg gccacttcat cgccgccgtc acttccgtga
      721 cataacgctc gtcggggtgg aggtagtagc tcgagcccca gtcgatgccg gcgaagcgca
      781 ctgctgccgc cgccacgagg atgaggaaga agaccaggcc gaacgtgatc cggaacggcc
      841 cgatgtagat cgcatcctga tcgtggccgg cttcgcggcg gcgttccgcg atccgcatcg
      901 tggacggagt gtgcggagcc ttgagcgtac gccggttgac gaacgttcgg cgcttacgca
      961 tcgcgagtgg aaggcgtgat cggttttcgg gcgcgaatgg caatcgcaac gaaggaatcc
     1021 ttgcggtcgt cgaggtcgga ggcgtcgttc ggggcgtcga tccattcgag cacccgcatc
     1081 gcgataccgg tcagtcccga tggtggtgga gcatcgtcca tccggcggtc tgcctgcttc
     1141 ttgagtttcg ctggcacgag ccctttctgg atcaatggct ttccgatacc gtacatcacc
     1201 aggtgggcga agggggggct gtagtgcgtg agtccccgag tctcctccac cgtgaagcca
     1261 tgaccggcga tcagcgcgcc gaggttcgac ttcgtgtaga gccgctgatg gccgtaccag
     1321 atgcccgacc agggacgctc gccacggagc tgccgcccgc tgatcctgtc gagtacccag
     1381 tttcccggat cccagccgaa cgggaacttc gcgctgggaa cggtgacggt gaggacaccg
     1441 cccggcttca gtacgcggaa catctcggcg acggcctttc cgtcgtctgg cagatgctcg
     1501 agaacttcgc tgcagacgat atgcgaga
//
LOCUS       DASTMD010000199         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_17773, whole genome shotgun sequence.
ACCESSION   DASTMD010000199 DASTMD010000000
VERSION     DASTMD010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17773"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1384
                     /locus_tag="VFQ54_02985"
     CDS             149..1384
                     /locus_tag="VFQ54_02985"
                     /inference="COORDINATES: protein motif:HMM:NF017810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HEU0163980.1"
                     /translation="MNATRRFITVGLLLLSFVLWGSSSITVHAQTATPKGAAPVKPIE
                     FSLNPKGESDGSFFDVEAKAGETKDLTVSFANLYDQPLTLRSYVADAYTVVNGGFGVR
                     DEGVVAKAPTTWMTYPAATYTLKPKEGKEIGFSVSVPRDAKPGSYISALVLRTAAPIP
                     IEGTEMFDQIIQKALAVVIRVPGAETPSFSIGSATYASNQAGTTVSVDITNTGNVFLK
                     PTGTFAVVDGSGRQVLTAPVAMGSVYTGDTTQLQFTFAQPLAPGTYTIELSLKDGTTI
                     ASAGTEPLAFTVAAAATATASPVQVTDLAVAPGPDATSPIFATITGTIVNTGDPVPNA
                     RLTVHVRKDGKLVEDFQLIPSLSLAQGDTPINQRYIPATGFTKGTWTFDLTLESVETS
                     NQAATVILDQAVPGSIIIP"
     gene            1628..>2176
                     /locus_tag="VFQ54_02990"
     CDS             1628..>2176
                     /locus_tag="VFQ54_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEU0163981.1"
                     /translation="MAENVTLTAPVAAELQPFVSDEALAFVAGLQRKFNARRLELLAA
                     RETRQRKLLDGGDLDFLEETAPIRADPDWRVAPAPADLNDRRAEITGPCDRKMVINAL
                     NSGAKVFMADLEDASSPTWHNVIDGQRNLHDAVRRTITFDNPDGRVYKLNGEIATLLV
                     RPRGWHLNERHILVDGEAASGSL"
BASE COUNT          408 a          717 c          668 g          383 t
ORIGIN      
        1 gatcgtgcga acagagagcc gtccccgaaa ggggggcggc tctttctttg tctgagggat
       61 cacgagttgt gggtccggca tcgtacactc catgcaagca tgcactgccg gttccatcgc
      121 gctcaacccg aggaaaacgt acgtgatcat gaacgccacc cgccgcttca tcaccgtcgg
      181 tctgctcctc ctcagcttcg tcctgtgggg cagctcctcc atcacggtcc atgcccagac
      241 cgcgaccccg aaaggcgcgg cgccggtgaa gccgatcgag ttcagcctga atccgaaggg
      301 ggagtccgac ggctccttct tcgacgtcga ggcgaaggcc ggagaaacga aagacctcac
      361 ggtttcgttc gcgaatctct acgatcaacc gctcacgttg cggtcctatg tcgcggacgc
      421 ctataccgtg gtcaacggcg ggttcggcgt gcgtgacgag ggcgtcgtcg ccaaggcccc
      481 gacgacgtgg atgacctatc cggccgcgac gtatacgctc aagccgaagg aaggaaagga
      541 gatcgggttt tcggtgagcg tgcctcgcga cgcgaaaccc gggagctaca tcagtgccct
      601 cgttctccgc accgccgcgc cgatcccgat cgagggcacg gagatgttcg atcagatcat
      661 ccagaaggcc ctggcggtgg tgatcagggt gccgggcgcc gaaactccat ccttcagcat
      721 tggctccgcg acctacgcgt cgaatcaggc gggaacgacc gtcagtgtcg atatcacgaa
      781 cacgggcaat gtcttcctca aaccaaccgg caccttcgcg gtcgtcgatg gttccggcag
      841 gcaggtcctc acagctccgg tcgcgatggg gagtgtctac acgggggaca cgacgcaact
      901 ccagttcacc ttcgcgcagc cgctcgcacc cggtacctat acgatcgagc tttcgctgaa
      961 ggatgggacg acgatcgcaa gcgccgggac agagcccctc gcgttcaccg ttgccgccgc
     1021 cgcgacggcg accgccagtc cggtccaggt caccgacctc gcggtggctc ccggcccaga
     1081 cgccacgtca ccgatcttcg cgacaatcac ggggacgatt gtcaacaccg gcgatcccgt
     1141 cccgaatgcg cgcctgacag tgcatgtgcg caaggatgga aagctcgtcg aggacttcca
     1201 gttgatcccc tccctctcgc tcgcccaggg agacacgccg atcaatcagc gctacatccc
     1261 ggcgacgggt ttcacgaagg gaacgtggac cttcgacctc acgctcgagt cggtagagac
     1321 atcgaatcag gcggcgacgg tcatcctcga ccaggcggtt ccgggatcga tcatcatccc
     1381 gtgacgtgac gtgacgtgac gtgacgtgac gtgacgtgac gtgacgtgac gtgacgtgac
     1441 gtgacgtgcc gacgccacgc cgtccggagt ggcgacgcag ggcagcggtg cggtcgattc
     1501 gtgatcgcac cgcgatcgtc atcctcttgc gcgacgtggg tctggagtgg aacatgatcg
     1561 cgtagcatct tccatattca acggtgatga caccgtggtc gtcaccggat caggagagat
     1621 ttcgatcatg gccgaaaacg tcaccctgac tgctcccgtc gccgcggagt tgcaaccgtt
     1681 cgtctccgat gaggccctgg ccttcgtcgc gggcctgcag cggaagttca atgcccgtcg
     1741 ccttgaactg ctggccgcgc gtgagacgcg ccagcggaaa cttctcgacg gtggcgacct
     1801 cgatttcctc gaggagacgg cgccgattcg ggccgatccc gactggcgtg tcgctccggc
     1861 accggccgac ctcaacgacc gccgcgccga gatcaccggt ccctgcgatc gcaagatggt
     1921 gatcaacgcc ctgaactccg gcgcgaaggt cttcatggcc gacctcgagg acgcttcttc
     1981 gccgacctgg cacaacgtga tcgacgggca gcgcaatctc catgatgcag ttcgccgcac
     2041 gatcaccttc gacaaccccg acggtcgcgt ctacaagctc aacggtgaga tcgcgaccct
     2101 gctggtgcgg ccgcgcggct ggcatctcaa cgagcggcac atcctggtcg atggcgaggc
     2161 agcctctggc agcctc
//
LOCUS       DASTMD010000200         1561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_197611, whole genome shotgun sequence.
ACCESSION   DASTMD010000200 DASTMD010000000
VERSION     DASTMD010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197611"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1242)
                     /locus_tag="VFQ54_02995"
     CDS             complement(55..1242)
                     /locus_tag="VFQ54_02995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU0163982.1"
                     /translation="MEPITVTIDRIVGDGKGIGFADGRTIFVARTAPGDVVKARVMRI
                     QGKVFHAEIAEIVTPSPLRIEPRVPDYDESGGCDLQHIGYADQLKVKAGIIEDSLRRI
                     AKIDPVPTVSVTPSPQEWGYRSRAEFQIDHEKKHVGYFAENSHRVVDVEESPVLTPEV
                     QVLLTTLRDDVDAGFVPTAAREYRAVQGDTGSLLEPTATQRSKQVMRTVGGDLYRFSG
                     ECFFQANVPIAEEITRHVLRIAEEARLTPGIAIDLYCGVGLFTVPLARRFKRTIGVES
                     YAPATKYAEINLSEANLDTARIVTSPVERWLAGDRTPLGRVALAVFDPPRTGAGPEVI
                     AAIARMKPTHIAAVSCDPATFARDIRGLLDGGYELVDVQGYDMFPQTHHVEIVGHLRR
                     IEA"
BASE COUNT          241 a          461 c          577 g          282 t
ORIGIN      
        1 tgacgcccgg tgggagcggc aacgtcagcg cgagcgtgac gggatcgcgc ttcgtcacgc
       61 ctcgatccgg cggagatggc cgacgatttc gacgtgatgg gtctgcggga acatgtcgta
      121 gccctggaca tcgaccagtt cgtagccgcc gtcgaggagg ccgcgaatgt cgcgggcaaa
      181 cgtcgcggga tcgcaggaga ccgcggcgat atgcgtcggc ttcatcctcg cgatcgcggc
      241 gatcacttcc ggaccggcgc cggtccgtgg cggatcgaag acggcgagcg cgacgcgccc
      301 gagcggcgtg cggtcgccgg ccaaccaccg ctcgaccggt gaggtgacga tccgcgccgt
      361 gtcgagattg gcttcgctga ggttgatctc ggcgtacttg gtcgccggag catagctctc
      421 gacaccgatc gtgcgcttga accggcgagc caggggaacg gtgaagagac cgacaccgca
      481 gtagagatcg atcgcgatcc ccggcgtgag gcgcgcttct tcggcgatgc ggagcacgtg
      541 gcgggtgatc tcttccgcga tcgggacgtt tgcctggaag aagcactcgc cgctgaaccg
      601 atagagatcg ccgccaacag tgcgcatcac ctgcttcgaa cgctgggtcg ccgtcggctc
      661 gagcaggctg ccggtgtcgc cctgaacggc gcgatattcg cgcgccgccg tcgggacgaa
      721 gccggcgtcg acatcgtcgc ggagggtcgt gagcaggacc tgcacctctg gcgtgaggac
      781 gggactctcc tcaacgtcga cgacgcgatg gctgttctcg gcgaagtagc cgacgtgctt
      841 cttctcgtga tcgatctgga actcggcgcg cgagcggtag ccccactcct gcggcgacgg
      901 tgtgaccgaa acggtgggca cggggtcgat cttcgcgatc cggcgaaggc tgtcctcgat
      961 gatgcctgcc ttcaccttca gctgatcggc gtatccgatg tgctggagat cgcaacctcc
     1021 gctctcgtcg taatcgggca cacgtggctc gatccggagc ggcgacggcg tcacgatctc
     1081 ggcgatctcg gcgtggaaga ccttgccctg gatgcgcatc accctcgcct tgacgacgtc
     1141 cccgggggcg gtgcgagcga cgaagatggt gcgaccgtcc gcaaagccga ttcccttgcc
     1201 atcaccgacg atgcgatcga tcgtgaccgt gatcggctcc atgtcgggaa cggcgcggtc
     1261 cgaggcgagt ggtcgtttcg gcgacggatg gttgccgtgg gctggtcgcg gttcgcgtgg
     1321 tgggcgttcc ggcgacggag cgacggcgtt ggcgttgtcg gggcgacctg gtcggcccga
     1381 tcccggccgt gttggtcggg cggggcgggg cgcggacggg cgcgagggac gggattgtcg
     1441 ggtggggggc atgcgtctgt cgtttcgttg aatggttggt ggaagcgggc aaaaggggtg
     1501 catcagaacc gatcctggca tgatgcatgt gccgtgatgt gtctattgta gcgggatagt
     1561 g
//
LOCUS       DASTMD010000201         5250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2518118, whole genome shotgun sequence.
ACCESSION   DASTMD010000201 DASTMD010000000
VERSION     DASTMD010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2518118"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="VFQ54_03000"
     CDS             complement(<1..1460)
                     /locus_tag="VFQ54_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0163983.1"
                     /translation="MTDWMPQHLLYSHESLLWLLVLVPVVALLPLLRNRRRRPSRLSI
                     VLRIVVLALLVFSIAGPLAAYDGNSVTTIFAIDRSNSIDPSANDAANSWVADAIGKAR
                     ADDAASIVTFGRQPSLAVPPESAGLVGNAWQQPGVDAVTNGDASDLSSALALARSLPV
                     GESRRIVLLSDGAENQGDALSQADQAASEGISIDVVPLAGVDAADLRLASVSGPTALW
                     QGDSLSVLATISVGAGGPVSIQLVIDGTVAATQNVTLQPGTTSYTLTHGALAPGFHAI
                     DVRVSGSAEIDRIAENDDGALGLVVRDRPSVLMIAPDGSDPSKLTSALQSQGAEVKVV
                     TPDDAPTTESGFAGYQAIVLNNVPAWTLSADQQTALVASTKDGGGLIAIGGSAAFGPG
                     SYAGTTLEQALPVTVKVTDGRQRPKVAVMIVVDKSGSMAYDPSEANTSKLDLAKQGIL
                     TAASALTTGDQIGVIAFNDEPTWALPMTTLTGSGDQ"
     gene            complement(1457..3472)
                     /locus_tag="VFQ54_03005"
     CDS             complement(1457..3472)
                     /locus_tag="VFQ54_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0163984.1"
                     /translation="MMTFLAPVGFVAGLSLIGIILLHMRRQVPATRSVPSLRFWTPAK
                     RDDLERTKLRKPPLSWLLLLQLLAALALTIALARPVSSGILGEFGGHTDPRHQIAILD
                     GSTSMQALIALGTDQTRFTAARGKVVDLLDSWQPGDITTVLVAGSQIQTYTATDQQQA
                     NTLRHFFGKLAPPGGRADMNAALRLAGDLVLPDRANQITMITDGAVTVDPAIAAGISA
                     PISLDDVGTGSETTNDAITDISARTDPTQRDHLRLAFTVSHFSDASATVPYTVQSDGN
                     DLANSTVTLGPDESRQIELSIPADASAVNVSIPQQDILLADNSASLQLQRDALSQLNV
                     MLVTDNPGDVQRALDAIPGTHVDTFPGSTPGLKAVSAGYDLAVFEGITPAADDLPSIP
                     MVLFQPPPLGDTFAIDGSMSAPTIDKVDAGASILNGVDLAGVTFSDVPTYTLPAGATE
                     IVGGLAADGTSGPLIWQGTLGGESYVATSFTTQSSNIGDRVAFPILMARTVQSLTKAQ
                     IPGALALGDPLVYSPSGDVVSLKIDTPSRGSETIAIPTSGASALDRTITFTDTSDSGV
                     YTIHELAADGSTLRQGSFVVNAGQRQESNLQPNPSLASSISQASASSGTTGSGAADHS
                     DLWPLLVAGALAAIVLEWLLVARRGGRRPSFEAPAATTTPGAEGGRS"
     gene            complement(3469..4458)
                     /locus_tag="VFQ54_03010"
     CDS             complement(3469..4458)
                     /locus_tag="VFQ54_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU0163985.1"
                     /translation="MNRIPRPLRLGPSRRQPAPGHPPSSVTSLYDEQLFARLRNLVIR
                     SRHLSASGLSGEHRSKRRGLSPEFSDFKPYSPGDDFRRIDWKTYARFDELFVRESETT
                     TEYDLHLLIDASPSMDWSGDESRPTKLRYAQQLAGALAYLSLWHFDRVTVGPIHTPDG
                     KVFGPIQGRNQIAPVFRYVERLQTAANTGEALAPSLARYVHARSRPGILIVISDCLDD
                     DPHELERSLRNLAARGWETTLVRIEDPAEVDPALLIEDGVNLELADRESGLRMDVHGA
                     QATFGQYREDRAAWSAAIDLLGVHQGTTVISARTDETVDSLIFTRLRALGVVR"
     gene            complement(4455..>5250)
                     /locus_tag="VFQ54_03015"
     CDS             complement(4455..>5250)
                     /locus_tag="VFQ54_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163986.1"
                     /translation="SQRAPTSQQPSSSDTGGQTDPGSGIAAAPGIASDSQPEQSQSSS
                     ASGSNSQDSSAPGSAPGSQGDQSQPSQSGDTNGAQGQQSSGSSSSSSTTTQSSSSQGS
                     GQPSQSTDGSQAEQSSQSASDSQSSTGSGASGQQSSDSSSQTQQGKPGANGQQGDDSK
                     TAQQGQASDDPPKGGDTSDAGDSDTGTNQNPNQSLVLQGTSDNDNVASGNDIGTSSTG
                     SGSGTGAASGSGTQGQVGPAGPDSNHVPDEYRDIVKGYFDGEGTSP"
BASE COUNT          829 a         1588 c         1913 g          920 t
ORIGIN      
        1 tgctggtcgc cgcttccggt gagggtcgtc atcgggagcg cccaggtcgg ctcgtcgttg
       61 aaggcgatga cgccgatctg gtctcccgtc gtcagtgcac tggccgcggt gagaatgccc
      121 tgcttggcaa gatcgagctt cgacgtgttc gcttcgctcg ggtcgtatgc catcgaaccc
      181 gacttgtcga ccacgatcat gaccgcgact ttcggacgct gccggccgtc ggtgaccttg
      241 acggtgacgg ggagcgcctg ctcgagcgtc gttccggcgt aggatcccgg tccgaatgcc
      301 gcggagccgc cgatcgcgat caggcctccc ccgtccttcg tacttgcgac gagggcggtc
      361 tgctgatcgg cactgagcgt ccaggcggga acattgttga gcacgatcgc ctggtagcct
      421 gcgaatccgg attcggtcgt tggcgcatcg tcgggcgtaa cgaccttgac ctcggcgcct
      481 tgcgactgga gcgcggacgt gagcttcgag ggatcggagc cgtccggcgc gatcatgagc
      541 acgctcgggc ggtcgcgaac gacgagcccg agtgcgccgt catcgttctc ggcgatccgg
      601 tcgatctcgg ctgatccgga aacgcggacg tcgatggcgt gaaatccggg cgcgagcgcg
      661 ccgtgggtga gcgtgtagga ggtcgtgccg ggctggagcg tcacgttctg ggtggcggcg
      721 accgtaccgt cgatcacgag ttgaatgctg accggaccac cggcgccaac ggagatcgtc
      781 gcgaggacgg agagcgagtc gccctgccag agagcggtgg gaccggagac ggaggcgagg
      841 cggagatcgg cggcgtcgac accggcgagg ggcacgacgt cgatggagat tccctcggat
      901 gcggcctggt cggcctgcga gagggcatcg ccctggttct cggcgccgtc cgacagcaga
      961 acgatccgac gcgactcgcc gaccgggagg gatctcgcaa gggcgagcgc cgaggagaga
     1021 tcgctggcgt cgccgttggt gacggcgtcc acgcccggct gctgccacgc gttgccgacc
     1081 aatccggccg attccggggg aacggcgagc gacggttgcc gtccgaaggt cacgatcgaa
     1141 gcggcatcgt ccgccctggc cttgccaatg gcatcggcga cccatgagtt cgcggcatcg
     1201 ttcgccgatg gatcgatgct gttcgagcgg tcgatcgcga agatcgtggt gaccgagttg
     1261 ccgtcgtagg cggcgagcgg tcccgcgatc gagaagacga gcagcgcgag aaccacgatg
     1321 cggagaacga tcgagagccg tgagggcctt cggcggcgat tcctcaggag cggcagaagg
     1381 gccacgaccg gcaccagcac gagtagccag aggagcgact cgtgggagta aaggagatgc
     1441 tgcggcatcc agtcggtcac gaacggcccc cttccgcccc cggtgtggtg gtcgcggcgg
     1501 gcgcctcgaa cgatggtctc ctgcctccgc gccgtgcgac gaggagccat tcgagcacga
     1561 tcgcggccag cgcaccggcg acgaggagtg gccagagatc ggaatggtcg gctgcgccgg
     1621 agccggtcgt tcctgacgac gccgaagcct gggagatact ggatgcaagc gacgggttgg
     1681 gctggaggtt ggattcctgt cgctgtcccg cgttcacgac gaacgatccc tgccgcaacg
     1741 tgctgccatc ggcagcgagc tcgtggatcg tgtagacgcc ggagtcagac gtatctgtga
     1801 aggtgatcgt ccgatcgagc gcggaggcgc ccgacgtcgg gatcgcgatc gtctcgctgc
     1861 cccggctcgg cgtgtcgatc ttcaggctga cgacgtcacc ggatgggctg tagacgagcg
     1921 gatcaccgag cgcgagcgcg cccgggatct gggccttggt gagggactgg actgtgcggg
     1981 ccatcaggat cgggaaggcg acacgatcgc cgatattcga cgattgcgtc gtaaaggacg
     2041 tcgccacata ggactcaccg ccgagcgtcc cttgccagat cagtggaccc gaggttccgt
     2101 ccgcagccag accgccgacg atttcggtgg cgccggccgg cagggtgtag gtcgggacat
     2161 cgctgaaggt caccccggcg agatcgacac cgttgagaat cgatgccccg gcgtcgacct
     2221 tgtcgatcgt tggtgcggac atcgaaccat cgatcgcgaa ggtatcgccg agcggtggcg
     2281 gttggaagag gaccatcggg atcgaaggga gatcatcggc ggccggggtg atcccctcga
     2341 atacggcaag atcgtaaccg gctgacaccg ccttcaatcc gggtgtcgat ccggggaagg
     2401 tgtcgacgtg ggtgccgggg atcgcgtcga gcgcgcgctg gacatccccg ggattgtccg
     2461 tcacgagcat gacgttgagt tgactcaggg catcccgctg gagctggagc gacgccgagt
     2521 tgtcggcgag gaggatgtcc tgctgcggga tcgagacgtt cacggccgag gcatcggcgg
     2581 ggatcgagag ttcgatctgc cggctctcat ccggtcccaa cgtcacggtc gagttggcga
     2641 gatcgttccc gtccgattgc acggtgtacg gcacggtggc ggatgcatcg ctgaaatggg
     2701 agaccgtgaa ggcgaggcga agatgatcgc gctgcgtggg atcggtgcgc gccgagatgt
     2761 cggtgatcgc gtcgttcgtc gtctcggatc cggtcccgac gtcgtcgagg gagatcgggg
     2821 cgctgatgcc ggcggcgatc gcgggatcga ccgtgacggc gccgtcggta atcatcgtga
     2881 tctgattcgc gcgatccggg aggacgagat ccccggcaag ccgaagggcg gcgttcatgt
     2941 cggcgcggcc accgggtggc gcgagtttgc cgaagaagtg ccgaagggtg tttgcctgtt
     3001 gctgatcggt tgccgtgtac gtctggatct ggctgccggc gacgagcacc gtcgtgatgt
     3061 ccccgggctg ccaggaatcg aggagatcga cgaccttgcc gcgggccgcg gtgaatcgcg
     3121 tctgatccgt ccccagcgcg atcagtgcct gcatgctcgt ggagccgtcg agaatcgcga
     3181 tctggtgccg gggatcggtg tgaccaccaa actcgccgag gatgccgctc gagactggcc
     3241 gggcgagggc gatcgtcagc gcgagcgcgg cgaggagctg gagcaggagc aaccagctca
     3301 ggggtggctt gcgcagcttc gtccgttcga gatcgtcgcg cttcgcgggt gtccagaagc
     3361 ggagggatgg caccgacctg gtcgcgggga cctgccgccg catgtggagg aggataatcc
     3421 cgatcaacga gagtccggcg acgaagccga ccggcgcgag gaacgtcatc atcgcacgac
     3481 tcccagggcg cggagacggg tgaagatgag gctgtctact gtctcgtcgg tacgggcgga
     3541 gatgacggtc gtgccctggt ggacgccgag cagatcgatt gccgccgacc aggcggcgcg
     3601 atcctcacga tactgcccga aggtggcctg cgccccgtgg acatccatgc ggagacctga
     3661 ctcacggtcc gcgagctcga gattgacgcc atcctcgatc aggagcgccg gatcgacctc
     3721 ggcgggatcc tcgatccgga ccagggtcgt ctcccagcct cgcgccgcga ggtttcggag
     3781 cgaccgctcg agttcatgcg gatcgtcgtc gaggcaatcc gagatcacga tcaggatccc
     3841 tggtcgcgac cgcgcgtgca cgtagcgggc gagtgacggt gccagcgcct ctccggtatt
     3901 ggcggctgtc tggagtcgtt cgacataacg aaagacaggg gcgatctgat tccgtccctg
     3961 gatcggcccg aagaccttcc cgtccggcgt atggatggga ccgacggtca cgcggtcgaa
     4021 gtgccagagc gagagatagg cgagtgcccc ggcaagctgc tgggcgtagc gcagcttcgt
     4081 cgggcgcgac tcgtcgccgc tccaatccat ggacgggctg gcgtcgatca acaggtgcag
     4141 atcgtattcg gtcgtggttt cactctcgcg gacgaagagt tcgtcgaagc gggcgtaggt
     4201 cttccagtcg atccggcgga aatcgtcccc gggggaatac ggcttgaagt cgctgaactc
     4261 cggtgagaga ccgcgtcgct tcgagcgatg ctccccggag agcccgcttg ccgagaggtg
     4321 ccgcgagcga atgacgagat tgcgcaggcg ggcaaagagc tgttcgtcat agagggacgt
     4381 cacgctggag ggcggatgcc ccggcgcagg ttgacgccgg gacggtccca ggcggagcgg
     4441 gcgcgggatg cggttcatgg cgacgttccc tcgccatcga aatagccctt gacgatgtcc
     4501 cggtattcat ccgggacgtg attgctgtcg ggtcccgccg gaccgacctg gccctgcgtg
     4561 ccggaaccgg acgccgcgcc ggtgccgctt cccgatcccg tgctcgaggt gccgatgtcg
     4621 ttgccggacg cgacgttgtc gttgtccgag gtcccctgaa gcacgagtga ctggttcgga
     4681 ttctggttcg tgccggtgtc gctgtccccg gcgtcactgg tatcgccacc cttcggtgga
     4741 tcgtcgctcg cctggccctg ttgtgcggtc ttgctgtcgt ccccttgctg cccgttggcg
     4801 ccgggtttgc cttgctgcgt ctgcgagctg ctgtccgacg actgctgtcc gctcgcgccc
     4861 gatccggtcg acgactggga atcgctcgcg gattgactcg actgctcggc ctggctgcca
     4921 tccgtcgact gcgagggctg tcccgatccc tgggaggaac tgctctgggt cgtggtcgac
     4981 gatgaggagg agcttccgct cgactgctgc ccctgggccc cgttcgtatc gccggattgc
     5041 gacggttgtg attggtcgcc ctgggacccg ggcgcactcc ccggagcgga ggagtcctgg
     5101 ctgttgctgc ccgatgcgct gctcgactgg ctctgctctg gctgcgagtc gctggcgatg
     5161 cccggcgcgg cggcgatgcc gctaccagga tcggtctgcc cgccggtgtc cgacgatgac
     5221 ggctgctggc tcgtgggcgc ccgctgcgac
//
LOCUS       DASTMD010000202         2925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1178599, whole genome shotgun sequence.
ACCESSION   DASTMD010000202 DASTMD010000000
VERSION     DASTMD010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178599"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VFQ54_03020"
     CDS             complement(<1..875)
                     /locus_tag="VFQ54_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0163987.1"
                     /translation="MVAQSEPSPDAGARTTGHDRLPRYAQPLVGRGRETNELQALFLR
                     TDEARPVSIVTLVGSGGVGKTRLAVRIATILLDHLADGAVFVPLASIDDPDLVPLAIA
                     DALGAGGFPPVAGDAEHRVRATLEHRAILIVLDNVEQIPGFADRLSTLSEQLPNVLWL
                     VTSRSPLHLAHEIVYPVAPLPIPDVDEPAAASPSIQLFASQARQANAAFEVTAANLPA
                     VRTICRRLGGLPLAIELAAARIRSFGPEEMTHILDRATGALDLLEDGSAEREERHSSI
                     REALAWSSSLLAPSA"
     gene            complement(940..1353)
                     /locus_tag="VFQ54_03025"
     CDS             complement(940..1353)
                     /locus_tag="VFQ54_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163988.1"
                     /translation="MAAAVTEIDLLVDRLVVEPMGWNKVWSLRTRIDIPFDDIVAIEQ
                     DRRLAENGPTGVRFPGANIPGVYLAGTFWKFWGDRKSRSFWLRRRADRCITIRLRNNS
                     FDYVTVDVADPAGEIRRITSSLHDRGIELGASGLA"
     gene            1661..>2925
                     /locus_tag="VFQ54_03030"
     CDS             1661..>2925
                     /locus_tag="VFQ54_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0163989.1"
                     /translation="MTTTFEPNPVPPNPLAANQPTESKNPIDRERLDLWRGVTAGGVA
                     GIIMLVTIILLRSFTGIISFLDVLADALLLVLPISWFAALLSLFGAQAKTLLLVGLMA
                     LLILLGAWVGRSYAHHTAGSRRAQWARAAAYGISMYAGFAAFTALFVSDQDPDLAAGG
                     GILKVLGLLGVAIIVYTVAMPVGLRILRRQDPAPRFLWGDEVANAADAEALAAGTYDR
                     RRLFTRIGFGIATIAGIGVLGKEVLRVANRKITSSENAGQMPSPITSNDDFYVISKNF
                     VDPSPQRGDDWSIEIAGLVTTPMTLKKTDLEAFGVQEFVSTLNCISNPVGGPLISTAK
                     WTGVPLAKVLRKAGVGNGAVKLIADADDGYQDSISIEKALSPEPFIVWEMNGENLPRL
                     HGVPVRLIVPGIYGMKNVKWLTKLTVTNQ"
BASE COUNT          579 a          936 c          915 g          495 t
ORIGIN      
        1 tgcgcggagg gcgcgagcag cgaactgctc cacgccagcg cctcgcgaat gctggaatgt
       61 cgctcctccc gctccgccga accatcctcg aggagatcga gggcgcccgt ggcgcgatcg
      121 aggatgtggg tcatctcctc gggcccgaag gaacggatgc gcgccgccgc gagttcgatc
      181 gcgaggggca gcccgccgag ccggcgacag atcgtccgca cggccgggag attcgccgcg
      241 gtcacctcga acgcggcatt tgcctgtcgc gcctgcgatg cgaagagctg aatggaaggc
      301 gatgcggcgg cgggttcatc gacgtccggg attggcagcg gtgcgaccgg atagacgatc
      361 tcgtgcgcga ggtgcagcgg ggagcgactc gtcacgagcc aaaggacatt cggcaactgc
      421 tcgctcagcg tcgagagccg gtcggcaaat ccgggaatct gttcgacatt gtcgagcacg
      481 atcaggatcg cccgatgctc cagcgtcgct cgcacgcggt gttcggcgtc accggcaacc
      541 ggagggaatc cccccgcccc gagcgcatcg gcgatcgcaa gcggaaccag gtcgggatcg
      601 tcgatcgacg caagcggcac gaagacggcg ccgtcggcca gatgatcgag aaggatcgtc
      661 gcgatccgca cggccagacg cgtcttgcca acaccgcctg atccaaccag cgtcacaatc
      721 gagacgggtc gcgcctcatc ggttctgaga aaaagcgcct ggagctcgtt cgtctcccgc
      781 ccacgtccga cgagcggctg cgcataccgg gggagacgat cgtgaccggt cgtcctggcc
      841 ccggcatctg gcgatggctc ggattgggcg accatgcgac ctgccttcca cgagcaacga
      901 tacgaagtgc gaacgagcag gaagaccgga gagcggtgat caggcgaggc cggaagcacc
      961 aagctcgatg ccacgatcgt gaagtgaaga cgtgatccgg cgtatctcac cagcaggatc
     1021 tgctacatca actgtgacat aatcgaagga gttattccgc aaacgaatcg tgatgcatcg
     1081 atcggcgcgg cggcggagcc agaaggaacg cgacttgcgg tctccccaga acttccagaa
     1141 cgtcccggcc aggtagacgc caggaatatt ggcgccaggg aaccggactc cggtcgggcc
     1201 attctcggca aggcgacgat cctgctcgat cgcaacgatg tcgtcgaacg ggatgtcgat
     1261 ccgggtccgg agcgaccaga ccttgttcca ccccattggt tcgaccacca accgatcgac
     1321 gagaagatcg atttccgtca ccgcagccgc catgccgtcc gagatccttt ccaaggaaga
     1381 acctgcccgg tatccaccca gtggcggaag gcgggcaggg aacaattcat accatcgtga
     1441 tgtcctgcgt agtcacaacc gtggatgatg atcccaaagg cgcgagacga cgtttatcag
     1501 aacataggat catcatcagt gccgggtttc cggcaccagt caagggtcag catatcagga
     1561 atgccgcggc aaatgacgcc gaatccatga gacacgttgc cacattctac gcgcggccca
     1621 catgcacgga ttcttcccgt gcgggtcgaa ggaacagacc gtgaccacaa ccttcgaacc
     1681 gaatcccgtt cccccgaacc ctctggcggc gaatcaaccc acggaatcga agaacccgat
     1741 cgaccgcgaa cgcctcgatc tctggcgggg tgtcaccgcc ggtggcgttg ccggcatcat
     1801 catgctggtg acgatcatcc tcctgcgatc cttcaccggg atcatctcct tcctcgatgt
     1861 tctcgccgat gcgctccttc tggtgctgcc aatctcctgg ttcgccgcct tgctcagcct
     1921 cttcggcgcc caggcgaaga ccctgttgct cgtcggcctg atggcattgc tgatcctgct
     1981 cggcgcctgg gtcggtcgct cgtacgcgca tcacacggcg ggatcccgcc gcgcacagtg
     2041 ggcgcgtgcc gccgcctacg gcatctcgat gtatgccgga ttcgccgcct tcacggccct
     2101 cttcgtcagc gaccaggatc ccgatctcgc cgcgggaggc ggcatcctga aggtcctcgg
     2161 tcttcttggg gtggcgatca tcgtctacac ggtcgcgatg ccggtgggat tgcgcatcct
     2221 caggcggcag gatcctgcac cccgcttcct ctggggcgat gaggtcgcca acgcggcgga
     2281 tgccgaggca cttgccgccg gaacctacga ccggcgtcgc ctcttcaccc ggatcgggtt
     2341 cgggatcgcg acaatcgccg gaatcggggt gctcgggaag gaggtcctcc gggttgcgaa
     2401 tcgcaagatc accagcagcg agaacgccgg gcagatgcct tcaccgatca cgtcgaacga
     2461 cgatttctat gtgatctcga agaacttcgt cgacccgagc ccgcagcgtg gcgatgattg
     2521 gtcgatcgag atcgccgggc tggtcaccac gccaatgaca ttgaagaaga ccgatctcga
     2581 ggcattcggg gtacaggaat tcgtctcgac actcaattgc atcagcaacc ccgtcggcgg
     2641 cccgctgatc agcacggcga agtggaccgg ggtcccgctc gcgaaggtcc tgcgcaaggc
     2701 cggcgtcggc aacggcgcgg tgaagctcat cgccgatgcg gacgacggat accaggactc
     2761 gatcagcatc gagaaggcgc tctccccgga accgttcatc gtctgggaga tgaatggcga
     2821 gaacctgcca cgcctccacg gcgttcccgt tcgtctgatc gtaccgggaa tctacggcat
     2881 gaagaatgtg aagtggctga ccaagctgac ggtcacgaac cagga
//
LOCUS       DASTMD010000203         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1268408, whole genome shotgun sequence.
ACCESSION   DASTMD010000203 DASTMD010000000
VERSION     DASTMD010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268408"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..825)
                     /locus_tag="VFQ54_03035"
     CDS             complement(235..825)
                     /locus_tag="VFQ54_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEU0163990.1"
                     /translation="MITTLLSGIGVSASCGLNATLPLLILALADRFSGTVNLDQPYDF
                     FSSSWGIIILLVILPIELIADKIARVDHANDLLHTVILPAAGALSMMAIASQDDHINP
                     VAALILGLVLGGGVHWFKTINRPLITIRTHGIGNPIVSVAEDLVAIVVTIVALFVPYA
                     VIVALPLLGWLLYRLYVRLRSGRSSLFGLLAGVNRV"
     gene            complement(941..1579)
                     /gene="lexA"
                     /locus_tag="VFQ54_03040"
     CDS             complement(941..1579)
                     /gene="lexA"
                     /locus_tag="VFQ54_03040"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU0163991.1"
                     /translation="MKELSRRQQAILDFIAAFLDENDYPPTIRDIQRELGISSTSVVD
                     YNLKVLEERDVIRRNRNISRGIELVGRVPMRAGVISIPVLGQIAAGIPIQAAMDLETA
                     ESVDTIELSPELAPDAGRNLFALRVKGYSMVDALINDGDIVVLKRQETCENGETVAVW
                     LEDEKETTLKKFYLEGNRVRLQPANVTMDPIYTAATNVAIQGKLVTVVRSIS"
     gene            1792..>2082
                     /locus_tag="VFQ54_03045"
     CDS             1792..>2082
                     /locus_tag="VFQ54_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU0163992.1"
                     /translation="MTASNVLEKLEFGEIQRRLSVHCHFSLAATRALEIGPSGDRRIV
                     RYLLDVTQEAVDLVNGFPQLTVGGARDIRDQLEKAEKGGRLMPPELLTVIDTI"
BASE COUNT          421 a          610 c          643 g          408 t
ORIGIN      
        1 ggcctcgttc aattccttga acttgtcctc ggcctgtgga tcctcccggt tgacgtccgg
       61 atggagcttg cgggccttcg cacggaaggc gcgcttgatc gcggcgtcat ccgccgtgcg
      121 ctcaattccg agtaccttgt agtaatcctt gaaatcaaca ggcatatgca tcaccccaga
      181 ctattggtct gagttgagta taaatcttga gtcgatacga cgcaaggttg gacgtcacac
      241 gcgattgacg ccggcaagca gaccgaagag cgacgaccgt ccgctccgga ggcgaacgta
      301 gagccggtag agcaaccagc cgagcagcgg cagggcgacg atcaccgcgt acgggacgaa
      361 gagcgcaacg atcgtgacga cgatcgcgac gaggtcttcg gcgacgctga cgatcggatt
      421 gccgatgcca tgggttcgaa tcgtgatgag tgggcggttg atcgtcttga accagtggac
      481 gccgccgccg aggacgaggc ccaggatcag cgcggcgacc ggattgatgt ggtcgtcctg
      541 gctggcgatc gccatcatcg agagcgcccc ggcggcaggc aggatcacgg tatgaaggag
      601 atcgttggcg tgatcgacgc gcgcgatctt gtccgcgatc agttcgatcg gcaggatcac
      661 gagaaggatg atgatgcccc aactggaaga gaagaaatca tacggctgat cgagattgac
      721 tgtgcccgag aaccgatccg cgagagcgag gatcagcagc ggcagcgtcg cattcaatcc
      781 gcacgacgcc gagaccccga tgccggagag gagggttgtg atcaacaatt caccccatga
      841 gctgaaagcc gcgtacagac acgacgacgc gttcgcgaga gtatacgcgg ctcattgccg
      901 accagaggtg atggatatcg ggcattgcgg cccggaaaga ctaactgatc gagcgaacga
      961 ccgtaaccag cttgccctgg atcgcgacgt tggtggcggc ggtatagatc ggatccatcg
     1021 tcacgttcgc cggctggagc cggacccgat ttccctcgag gtagaacttc ttgagggtcg
     1081 tttctttctc atcctcgagc cagacggcga ccgtctcgcc gttctcgcag gtttcctggc
     1141 gcttgaggac aacgatgtcg ccatcgttga tgagcgcatc gaccatcgaa taccccttga
     1201 cgcggagcgc gaagagattg cgaccggcgt cgggggcgag ttccgggctc agctcgatcg
     1261 tgtcgacgga ctcggcagtc tcgaggtcca tcgctgcctg gatcggaata ccggcggcga
     1321 tctggccgag aacgggaatc gagatcacgc cggcccgcat cgggacgcgc ccgacaagtt
     1381 cgatgccgcg cgagatattg cggttgcgcc ggatgacatc ccgctcctcg agcaccttca
     1441 ggttgtagtc gacaacactg gtcgaggaga ttccgagctc gcgctggatg tccctgatcg
     1501 tcggcggata gtcgttctca tcgagaaacg ccgcgatgaa gtcgagaatc gcttgttggc
     1561 gtcgagagag ttctttcatc atcggagtcc tctatctcta gggatttcgc gggcggcagc
     1621 gtgcgggtat tcgctccggg tatgattgtc taccgatgcg attatagtaa cgaacacttg
     1681 ttcgtgtcaa tctctggcat tccctgaata cgttctagcc gtgcattctg tacgatcctg
     1741 ctgacactgc cttccgcgcc cgaccccgca ccctctggac tggaatctct tgtgaccgca
     1801 agcaacgttc tcgagaaact tgagttcggc gagattcaac gccgtctctc cgtacattgt
     1861 cacttcagcc tcgccgcgac ccgggccctc gaaatcggcc cgagcggcga ccgccgcatc
     1921 gtccgctacc tcctcgatgt cacgcaggag gcggtcgatc tcgtcaacgg ctttccccag
     1981 ctgaccgtcg gcggcgcgcg ggatattcgc gatcaactgg aaaaggccga gaagggcgga
     2041 cgcctgatgc cgcccgaact gctcacggtc atcgacacga tc
//
LOCUS       DASTMD010000204         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1536458, whole genome shotgun sequence.
ACCESSION   DASTMD010000204 DASTMD010000000
VERSION     DASTMD010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536458"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..577
                     /locus_tag="VFQ54_03050"
     CDS             65..577
                     /locus_tag="VFQ54_03050"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU0163993.1"
                     /translation="MTRSATGRIASSPAGQSIVLERTFNAPIGDVWKSVTEPERMNRW
                     IGTWKGTAGTGKQVSFAMTAEGDVDYHPVDILLCDEPHQLIVDSIIPGFGTWHLTLEL
                     VENEGVTTLTFSHLLASEENVGSFAAGWEYYIDRLVATYTGEEFADFGEYYPDQSPYW
                     EEAYAAAVQG"
     gene            complement(582..1022)
                     /locus_tag="VFQ54_03055"
     CDS             complement(582..1022)
                     /locus_tag="VFQ54_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEU0163994.1"
                     /translation="MSDPAALTDIRDRDDIAELVVAFYTSAFADEQLGPIFTDIVKLD
                     LDAHLPYICDFWETVVFQAGLYRRDSLAIHRDVNDLVPLTKEHFQRWEDLWHETVDAR
                     FAGPRAIVAKVHASRMAVSMQNRLKLGVVERLIQIGPFPFPQDR"
     gene            complement(1015..1341)
                     /locus_tag="VFQ54_03060"
     CDS             complement(1015..1341)
                     /locus_tag="VFQ54_03060"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0163995.1"
                     /translation="MAARTVEHPPACDITAVDVFASLGDQTRIAIVRTLCAAGGEMPW
                     GSFDLPIARSTLSHHLKVLRNAGIVRCRQDGTRSFVSLRLDDLDRRFPGLLQIVLSDT
                     EVPAHE"
     gene            1458..>2036
                     /locus_tag="VFQ54_03065"
     CDS             1458..>2036
                     /locus_tag="VFQ54_03065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEU0163996.1"
                     /translation="MALSLSILDLAPVVEGSDVRTALEDSLVLAQHAEKFGYARYWVA
                     EHHNMIGVASSATSLLIGYLAAGTSKIRVGSGGIMLPNHSPLVIAEQFGTLETLYPGR
                     IDLGVGRAPGSDGYAMRAMRRDPRAGDEFPQDVQELQILLGSGIPNQAVFATPGRDTN
                     VPIYILGSSMFGAQLAAMLGLPYAFASHFAPDA"
BASE COUNT          393 a          662 c          626 g          355 t
ORIGIN      
        1 agtcggaggc cgccaccaac ccgaagccac cactgtccac gatcaacgca aaggagcgat
       61 ccgcatgacc cgatccgcca ccggacgaat tgcctcatct cccgcgggcc agagcattgt
      121 gcttgagcga accttcaacg ccccgatcgg cgatgtctgg aagagcgtga ccgagcccga
      181 acgcatgaat cgctggatcg gcacctggaa gggaaccgcc gggaccggca agcaggtctc
      241 attcgcgatg accgcggaag gcgacgtcga ctatcatccg gtcgacatcc tcctttgcga
      301 cgagccccat cagctgatcg tggactcgat cattcctggt ttcggcacgt ggcacctcac
      361 gctcgagctc gtcgagaacg aaggcgtgac cacgctgacg ttcagccacc tgctggcttc
      421 tgaagagaat gtcggttcat tcgcggcggg atgggaatac tacatcgacc gtctggtcgc
      481 gacctatacc ggcgaggaat tcgccgactt cggcgagtac tatcccgacc agagcccata
      541 ttgggaagaa gcgtacgccg ccgccgtgca ggggtagata cctaccgatc ctgcgggaac
      601 gggaatgggc cgatctggat caggcgctcg acgacgccga gcttgagccg attctgcatc
      661 gagaccgcca tccgcgaggc atggaccttg gcgacgatcg ccctggggcc ggcgaagcgc
      721 gcgtccaccg tctcatgcca gagatcttcc cagcgctgga aatgctcctt cgtcagcggg
      781 accagatcgt tgacgtcccg gtgaatcgcg agcgaatccc tgcggtagag accggcctgg
      841 aagaccaccg tctcccagaa gtcacaaata tatggaagat gggcatcgag atccagcttc
      901 acgatgtccg tgaagatcgg cccgagttgc tcgtctgcaa acgcgctcgt gtagaacgcg
      961 accacgagtt ccgcgatgtc gtcgcgatcg cggatgtccg tcagcgcagc cggatcactc
     1021 atgggcggga acctcggtat cactgagcac gatctggaga aggccgggga agcgacgatc
     1081 gagatcgtcc agccgaagcg agacgaaact gcgcgtgcca tcctgccggc accgcacgat
     1141 tccggcgttg cgaagcacct tgagatgatg gctcagggtc gatctcgcga tcggaagatc
     1201 gaacgatccc cagggcatct ctccgcctgc cgcgcagagc gtccgcacta tcgcgatccg
     1261 ggtctggtcg ccgagcgagg caaagacatc cacggcggtg atatcgcatg ctggtggatg
     1321 ttcgacggtt ctggcagcca tgacgctccc cgcagcgccg gtctcgacgc gccaatcaac
     1381 agatggtagt gtaaatcatt cgatgttcga tgaacgacga acatccagcg acttttcgcc
     1441 gacaccgagg cactgacatg gctctctcgc tttcgattct cgatctcgcc cccgtcgtgg
     1501 agggctcgga cgtgcgcacg gccctcgagg acagcctcgt gctggcacag cacgccgaga
     1561 agttcggata cgcgcggtac tgggtggcgg aacaccacaa catgatcggc gtcgcctcgt
     1621 cggcgacctc gctgctgatc ggctacctcg ccgccggcac gtcgaagatc cgcgtcggct
     1681 cgggcgggat catgctgccg aaccactccc ccctcgtgat cgccgagcag tttggcacgc
     1741 tggagacgct gtaccccggc cggatcgatc tgggcgtcgg gcgtgccccg ggctcggacg
     1801 gctacgcgat gcgggcgatg cgccgcgatc cccgcgcagg ggacgagttt ccccaggatg
     1861 tccaggaact ccagatcctg ctcgggtccg gaattccgaa ccaggccgtc ttcgcgacgc
     1921 cgggacgcga tacgaacgtg ccgatctaca tcctcggttc gagtatgttc ggcgcgcagc
     1981 tggcggcgat gctcggcctc ccctatgcct tcgcctctca cttcgctccg gacgcc
//
LOCUS       DASTMD010000205         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_732870, whole genome shotgun sequence.
ACCESSION   DASTMD010000205 DASTMD010000000
VERSION     DASTMD010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732870"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFQ54_03070"
     CDS             complement(<1..530)
                     /locus_tag="VFQ54_03070"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEU0163997.1"
                     /translation="MKSQGLPTYLVEPGKKFDLAKWETSTRGPFDDEEDSLAEFDHQR
                     KRIQSYQERLWAEHKQSLLIVLQAMDTGGKDGTIRHVFAGVNPQGCRVSSFKQPSAEE
                     LDHDFLWRIHQRTPGKGIITIFNRSHYEDVLVVRVHKIAPKPVWKSRFDQINEFEKIL
                     TENGTTILKFFLHISR"
     gene            complement(973..1095)
                     /locus_tag="VFQ54_03075"
     CDS             complement(973..1095)
                     /locus_tag="VFQ54_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0163998.1"
                     /translation="MTENERRAQDVLTREGLMLRSISAVGLIAMFLLVLLVVNP"
     gene            complement(1439..>3032)
                     /locus_tag="VFQ54_03080"
     CDS             complement(1439..>3032)
                     /locus_tag="VFQ54_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEU0163999.1"
                     /translation="VNEPAPETPQATAPAVSPELPVSATTQHAVTDASLPEGTIVAES
                     GDATAAAIVAPGKKSAMVSTADWFDDRLGVREWLWPIATHLSPKVNWWYVFGSATLIA
                     FVMQIVTGVALAFTYVPSPNSAYQSLQWITDDATLGSVVRGIHYWGASAMMVLVFIHM
                     CQVFAFGSFKFPRELNWLTGVLLLLLTLALAFTGQLLRWDQDAYWAVYVVSEQVARVP
                     FIGNWLMQIIIAGESVGGNTLTRFYATHVFLLPAATMGLIGVHLYLVIRHGISEMPEP
                     GKLVDPKTYREEYHKLMEEDGEPFWPDAAWKDVVFAVLVGAVVLILAIYPGPKHLGEV
                     ADPTNLNAHPRPDWYFQWLFAVLALMPPKMEDYLIIIGPLLAIVALFAVPFIANRGER
                     HWGRRPWALGIISFSVIAFVILTVLAFKAPWSPDLDPGPLSPAVEARAEQAGLAKGAE
                     IFQNDACINCHRVGDVGGDKGPNLTYIGDDLTQEQLTWRILNGGNGMPAYGETLSTSD
                     VNAVVQFLLQQKKEHEDEDATP"
BASE COUNT          560 a          922 c          977 g          573 t
ORIGIN      
        1 tcccgcgaga tatgcaggaa gaacttgagg atcgtcgtcc cgttctcggt gagaatcttc
       61 tcgaattcat tgatctggtc gaagcgggat ttccagaccg gcttcggcgc gatcttgtgc
      121 acgcggacga cgaggacatc ctcgtaatgc gagcggttga agatcgtgat gatgcccttg
      181 cccggtgtcc gctggtggat gcgccagaga aaatcgtggt cgagctcttc cgcgctgggc
      241 tgcttgaagg atgagacgcg acatccctgc ggattcactc ccgcgaagac atgacggatg
      301 gtgccgtcct tgccgcctgt atccatcgcc tggaggacga tcaggagcga ctgcttgtgc
      361 tcggcccaga gacgttcctg gtacgactgg atgcgcttcc gttggtggtc gaactcggcg
      421 agcgaatcct cctcgtcgtc aaacggaccc ctggtggacg tctcccactt cgcgagatcg
      481 aatttcttgc cgggctcgac gaggtaggtg ggcaatccct gacttttcac ggtgatcctt
      541 tcggctggcg tgcgtcgcgg tagggtggcg atgcgacttc gcgatctcgt gggcgaactg
      601 aaccacaatc gttaaccaga gatcaacccg gacctgcggc gtgccgcgaa atccgctgtc
      661 gtggcgtcgg gcccccgggg gatgcgccgt gcatgtcgtt tggtcccggg gtgaagcccc
      721 ggtgacgccg cggaactcgt gatccgggac tccgcccagg gcggccgcac gggccgtgtc
      781 catgcgatcg acgttgagcg aagggagttc acgcggagac gatcgtaccc ggctgtgccc
      841 cacccgtcac agagcgtgac tcgcggaggg gatgcggtga aatgaaatga gggctccgcc
      901 ataccggcgg agccctcatc ggtgtcatga tgtgtcacac gtgtggtcgt gtgttgttga
      961 tgacgacgtt gcttacggat tgaccacaag cagtacgagc aggaacatcg cgatcaggcc
     1021 aacagccgaa atacttcgca gcatcaaccc ttcgcgcgtc aggacatcct gcgcccgtcg
     1081 ctcattctcg gtcatggtat tgaatctccc cctgggtcat cgcagacgag ctacccgcca
     1141 tcctcggcgg gatccttcga cctgcaagag cttcgcgtgt cgcgcctcaa ccgagacgag
     1201 tccttgtcct gtccctcaac aagacttcgg ggcgaagtgt acgtacgccg taacaactct
     1261 gctaagagca taggcggttc gtgccgccat gtcaatataa cgagcaacat gcacaaacgg
     1321 tttcggtgcg atcgaatgcg ttttgttagg tgtaatcgta cgtacgtacg cggttcggct
     1381 ccgcgcgcga cagccgcacg tacagagccg aaccggaaaa tccccggtac gcgttcactt
     1441 atggcgtcgc gtcttcgtcc tcgtgttcct tcttctgctg aagcaggaac tgcacgaccg
     1501 cgttgacgtc cgaggtcgag agcgtctcgc cgtaggccgg catcccattg ccgccgttga
     1561 gaatgcgcca ggtgagttgt tcctgtgtca ggtcgtcacc gatataggtg aggttggggc
     1621 ccttgtcgcc accaacatct ccgacgcggt ggcagttgat acaggcgtcg ttctggaaga
     1681 tctcggcgcc tttcgccaac cccgcctgtt ccgcccgcgc ctcgactgcc ggcgagagcg
     1741 gacccggatc gagatccggc gaccacggcg ccttgaatgc gagcaccgtc aggatcacga
     1801 acgcgatcac gctgaaggag atgatgccca atgcccaggg acgtcgaccc cagtggcgct
     1861 cgccacggtt ggcgatgaac ggcaccgcga agagcgcgac gatcgccaga agcggaccga
     1921 tgatgatcag gtagtcttcc atcttcggcg gcatcagggc gagtaccgcg aagagccatt
     1981 ggaagtacca gtcgggacgc ggatgcgcgt tcagatttgt cggatcggca acctcgccca
     2041 ggtgtttcgg acccgggtag atcgccagga tcaggacgac cgcgcccacc agcaccgcga
     2101 agacgacgtc cttccaggcg gcgtccggcc agaacggctc gccgtcctcc tccatcagct
     2161 tgtggtattc ctcgcgatag gtcttcggat cgacgagctt gccgggctcc ggcatctccg
     2221 agattccgtg acggatcacg aggtagagat ggacgccgat gagtcccatc gttgccgcgg
     2281 gaaggaggaa gacgtgcgtc gcgtagaagc gcgtcagtgt gttccctccc accgattcac
     2341 cggcgatgat gatctgcatc agccagttac cgatgaacgg cacgcgcgcg acctgttcgg
     2401 agacgacgta gaccgcccag taggcgtcct ggtcccagcg aagcagctga cctgtgaagg
     2461 cgagcgcgag ggtcaggagg agcaggagca caccggtgag ccagttcagc tcgcgcggga
     2521 acttgaagga cccgaacgcg aagacctggc acatgtggat gaagacgagc accatcatcg
     2581 cgctggcgcc ccagtagtgg atgccgcgga cgacgctgcc gagcgtcgca tcgtcggtga
     2641 tccactggag gctctggtag gcgctgttcg gtgacggcac gtaggtgaac gcgagcgcga
     2701 cgccggtcac gatctgcatc acgaaggcga tgagcgtcgc gctgccgaag acgtaccacc
     2761 agttcacctt cggggagagg tgggtcgcga tcggccagag ccattcgcgg acgccgaggc
     2821 gatcgtcgaa ccagtccgcg gtcgagacca tcgccgactt cttgccaggc gccacgatcg
     2881 cggccgcggt cgcatcgccg ctctcggcga cgatcgtgcc ttccggcagc gacgcgtctg
     2941 ttacggcgtg ctgcgtcgtg gccgataccg gcagctccgg tgagaccgcg ggcgccgtcg
     3001 cctggggcgt ctcgggagcg ggctcgttta cc
//
LOCUS       DASTMD010000206         5266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_465384, whole genome shotgun sequence.
ACCESSION   DASTMD010000206 DASTMD010000000
VERSION     DASTMD010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465384"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..622)
                     /locus_tag="VFQ54_03085"
     CDS             complement(302..622)
                     /locus_tag="VFQ54_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164000.1"
                     /translation="MIQSLIDWYKAKNRHDRNVIGGFVIGLSFGLVFILLSPTIWYAG
                     LIGGIILGIIMAKLFALDADHDMQEQRVSRLANPVLTQRLGTQPAGNTSTPATPADPV
                     DHEA"
     gene            834..1520
                     /locus_tag="VFQ54_03090"
     CDS             834..1520
                     /locus_tag="VFQ54_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU0164001.1"
                     /translation="MSKKVDRRPKAVIWDLDGTLIDSEEWHWLSWQEILHAEGYPITY
                     EEFLKGFGRRNDEVLREYFGADADPARLLQIGEDKEARYRQYIEQHGIVLMPGVEDWL
                     GYLGESGWLMGVATSAPNRNLNIILDVSGLALYFDAIVGKDDVTNGKPDPEVFLTAAS
                     RLEVPPVRSIVVEDVVAGIAGAHAAGMAAIGVGERHAELGADLSVPRLDELEDDAFET
                     LLAAHSATES"
     gene            2140..2730
                     /locus_tag="VFQ54_03095"
     CDS             2140..2730
                     /locus_tag="VFQ54_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU0164002.1"
                     /translation="MAEVSEKPATKAAGLAANEDHWTVAADSNVQTADAVLFRDTINS
                     LLLLLPTIIRRMGAIVGECPAGYNPTLAQKRVMCYVAVRGKATVSQIAEALDMSRPGA
                     SEMIDRMVEIGYVMRESNPADRRQVLISLTPDAATIVDALTSRRREVLIESLGDLDNE
                     ELRGFLKGMHGMARVLAPDYSSEVEAVSLRNCPTEQ"
     gene            2872..4512
                     /locus_tag="VFQ54_03100"
     CDS             2872..4512
                     /locus_tag="VFQ54_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU0164003.1"
                     /translation="MATSRSSTNAPARETNPWAVLVVLCTAVFMLLLDTTIVNVAQRK
                     IQLGLDANFSQIQWVMDAYILAFAVLLLTFGRLGDIFGRRLLFAIGLVVFTASSALCA
                     ASSGLGDVFGISGANALIISRVIQGIGGALMMPQSLSMLTVVFPAEKRGAALGIWGSI
                     VALGAVIGPVLGGYIVTNFAWEWVFIINIPVGIAAVIGTIRLVPESTDPNASRSIDWG
                     GVLLSGAGIFALVYALIEGPRRGWTDSLILGLFVASAVLMVAFVIWEIRYRDPIMKLE
                     LFRIRNFWVANVLGAIISFGVMGMLFPLSAFLQVALGFSPIRSGLATVPIAASMMLLA
                     PFVGRLSEKYGPRWFLVTGYALMTVGVATTIMQISLHTDWQHLFLPMVITGIGMSFSF
                     PPMTTMALRQVPPRIAGSASGMINTTRNIGQVLGIAVLGSVLQSRMAADGKSQLAGVG
                     IDQGTRDQIIDLAKSNRFDEVVKVIPADQQQLIPQLLNALQHTFADAVQTTLWVAVAV
                     TAVGAVVALAVKNPVRQPVTQANPTPEGEKAAPVFVLE"
     gene            complement(4654..>5266)
                     /locus_tag="VFQ54_03105"
     CDS             complement(4654..>5266)
                     /locus_tag="VFQ54_03105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0164004.1"
                     /translation="NLEIPSKMTNSTLVRIETPVGAITVALDGEHTPITTANFLHYID
                     GGFYTGGRFHRTVRTDNNSNAGLRISDTDGGIDPNADRRQLPNDSIVIEVIQGGVNPD
                     RIGEQAGPIALERTSETGLRHLDGTISMARSTPDSAISDFFICVNDQPSLDFGGLRNP
                     DGQGFAAFGQVVEGMDVVRAIQTSPSEGQSLTPPVAIVGITRV"
BASE COUNT          917 a         1574 c         1738 g         1037 t
ORIGIN      
        1 cggcgatgcc gtaggaatcg gtgatcagtt gaacgagcag tacgcaggcg agcgcccgca
       61 tcgcgagtgg gatacgtccg acgaaaccgg ccacgaaaaa tccgaacgct cccggctcag
      121 ccagaagcgc acgaaaagtc ggggcgtcag ttgtcgacgt cgtctgcgtc atattgcctt
      181 gttgttgtgc ggatacggat cgaatcgtgc caatgtcaca gtatcacacg cgccgggacg
      241 gatggtgtca tcgcgtcgcc ggtcgcgtgt ggagggtgac tggcagaatc ggaagggacg
      301 gtcaggcctc gtggtcgacc ggatcggcgg gcgtagcagg ggtactcgtg tttccggctg
      361 gctgcgtgcc gaggcgctgg gtcaggaccg gattcgcgag ccgggagacc cgctgttcct
      421 gcatgtcgtg atcggcgtcg agggcgaaga gcttcgccat gatgatgccg aggatgatgc
      481 cgccgatgag accggcgtac cagatcgtgg gcgagaggag gatgaagacc agcccgaacg
      541 agagaccgat cacgaatcca ccgatcacgt tgcgatcgtg ccgattcttt gccttgtacc
      601 agtcgataag agattgaatc atggatatca gctccccgcc gcgtcttgac gtgccctgtg
      661 cccgacgctc atggatcgcg gcacgttgcg ggtattgtac cgggactttg ccaaagcaga
      721 attccctgga gcgcgattcg tgatggcgtt ccgcagctga tgtgcgatgc tttttcgctt
      781 gagagttgat cgacgaccac gacgtaccac tcgggatgaa agcgagcgca gtgatgagca
      841 agaaggtgga ccgccggccg aaggcggtga tctgggatct cgatggcacc ctgatcgatt
      901 ccgaggagtg gcattggctg agctggcagg agatcctcca tgccgagggc tatccgatca
      961 cgtacgagga attcctcaag gggttcggac gtcgtaacga cgaggtcctt cgcgagtatt
     1021 tcggcgccga tgccgatccc gcgcgactcc tccagatcgg ggaggacaag gaagcgcggt
     1081 accggcagta catcgagcag cacgggatcg tcctgatgcc gggtgtcgag gattggcttg
     1141 gctacctcgg cgaatcgggc tggctgatgg gtgtcgccac ctcggcgcca aaccggaacc
     1201 tgaacatcat tctcgatgtc tccgggctgg cgctctattt cgatgcgatc gtcgggaaag
     1261 atgacgtgac gaacgggaag ccggatccgg aggtcttcct gacggcggcg tcgcggctcg
     1321 aggtgccgcc ggtgcgatcg atcgtggtgg aggatgtcgt tgcgggtatc gccggcgcgc
     1381 atgcggcggg gatggcggcg atcggggttg gggagcgtca cgccgagctg ggtgcggacc
     1441 tctccgtgcc gcgtcttgac gagctcgagg acgatgcgtt cgagactctg ctcgccgccc
     1501 actccgccac ggaatcctag ccactgcctg gcgccaacgc atcatcccag catccagggt
     1561 acgtcctccc gacacccagg acccgtcctc ccggcacccg ggaaacgtcc tcccgagctt
     1621 gtcgagggac cagttggcgc tcacgtgctt ccctcacgag gtcgtacaaa acacgtcctc
     1681 ccgagcttgt cgagggatca gccggcgtcc gcgtgctccc ggcaagacgc gtcgccagcc
     1741 ggggtggaac ggggagcatc gcgcatggga cgtggtcccg ggcgaacacc aggttcgccc
     1801 ctacggacgc gcgtctgacg gcggcgcgtc ggcgctccgc ccgggatgac atgcgaggga
     1861 atgctgcggg acgcccgttt ccaacgatcc ggaactgcga caagcgccgt ggggacgggc
     1921 cccatatggc gggcgacgaa aaggggacag gatctgtctc catttgggtc tacgatcgga
     1981 gtcagattca acgcggaatc cggcttcctg gtcgccgctg accagttcgt tgcggcctct
     2041 tgccaaagtc gttcggcagg tatatgattc gggtcacgaa tggttcggaa gacgactcat
     2101 tcggatggcg aacggaatca gttcaggagg atgcgacaca tggcagaggt atcggaaaag
     2161 ccggctacga aagccgccgg gcttgctgcc aatgaggatc attggaccgt tgctgcggat
     2221 tccaatgtcc agactgcgga tgcggtgctg tttcgggaca cgatcaattc cctgctgctc
     2281 ctgctgccaa caatcatcag gcgaatgggc gcgatcgttg gggaatgtcc cgcaggctat
     2341 aacccgaccc tggcccagaa gcgggtgatg tgctatgtcg cggtgcgcgg caaggcaacg
     2401 gtcagccaga tcgccgaggc gctcgacatg tcgagaccgg gagcatccga gatgatcgat
     2461 cgcatggtcg agatcggcta cgtgatgcgg gaatcgaatc ccgcggaccg gcggcaggtc
     2521 ctcatctcgt tgacgccgga cgccgccacg atcgtcgacg cgctcacttc caggcgccgc
     2581 gaggtgctga ttgaatcgct gggtgatctg gataacgagg aacttcgcgg tttcctgaag
     2641 ggcatgcatg ggatggccag ggtcctggcc ccggattatt cctcggaagt cgaagcggtt
     2701 tctctcagga attgccccac ggaacaataa cgagtctccc tcttcacgcc accgaccgca
     2761 gcgtcgaacg tggcaatccc tctctcgaat gctcccgaac gagcctggca cgacaggctc
     2821 tgggatggtg ttgtctctgt cagataccgg tctcctcgga aggacgtgaa cgtggctact
     2881 tcccgatcat caacaaacgc accagccagg gagacgaatc cctgggcagt gctggtggtg
     2941 ctctgcactg ccgtcttcat gttgctgctt gacacgacga tcgtgaatgt cgcgcagcgc
     3001 aagattcagc tcggtctcga cgcgaacttc tcccagatcc agtgggtgat ggacgcctat
     3061 atcctcgcct tcgccgtgtt gctgctgacc ttcggtcgcc tcggtgacat tttcgggcga
     3121 cgactgctct tcgcgatcgg tctcgtcgtc tttaccgcgt catccgcgct ctgtgccgcc
     3181 tcgagcggtc ttggggacgt cttcgggatc tccggcgcaa acgcactgat catctcccgc
     3241 gtgatccagg gcatcggcgg cgcgttgatg atgccacagt cgctttcgat gctcaccgtc
     3301 gtcttcccgg cggagaagcg cggcgccgcg ctcggcatct ggggcagcat cgtcgccctt
     3361 ggcgccgtga tcggcccggt gctcggtggc tacatcgtga ccaacttcgc ctgggagtgg
     3421 gtcttcatca tcaatatccc ggtcgggatc gccgccgtga tcgggacgat ccgcctcgtg
     3481 ccggaatcga ccgatccgaa cgcatcacgt tcgatcgact ggggcggtgt attgctctcc
     3541 ggagccggca tctttgccct cgtctacgcc ttgatcgagg gaccgcgtcg cgggtggacg
     3601 gatagcctga tcctcggtct cttcgtcgct tccgccgtgt tgatggttgc cttcgtcatc
     3661 tgggagatcc gttaccgcga cccgatcatg aagctggagc tcttcaggat ccgcaatttc
     3721 tgggttgcga acgtgctcgg cgcgatcatc tcgttcggcg tgatgggcat gctcttcccg
     3781 ctcagcgcct tcctccaagt tgcgctcggc ttctcgccga ttcgttccgg tcttgcaacg
     3841 gtgccgatcg cggcttcgat gatgctgctg gccccgttcg tcgggcgctt gtccgagaaa
     3901 tacggcccgc gctggttcct ggtgaccggc tacgcgttga tgacggtcgg tgtcgccacc
     3961 acgatcatgc agatcagcct gcataccgat tggcagcacc tcttcctgcc gatggtgatc
     4021 acgggtatcg gcatgagctt ctcgttcccg ccaatgacga cgatggcgct gcggcaggtg
     4081 ccgccgcgga tcgccggcag cgcctcgggc atgatcaaca cgacccgtaa catcgggcag
     4141 gtgctcggca tcgcggtact gggctcggtg ctccagtcac ggatggcggc agacggcaag
     4201 tcgcagcttg ccggcgtcgg gatcgaccag ggaacgcgcg accagatcat cgatctggcg
     4261 aagtcgaacc gcttcgacga ggtcgtgaag gtcatcccgg cggatcagca gcaactgatt
     4321 ccgcaactgt tgaacgcgct gcagcacacc ttcgccgatg cggtgcagac gacgctctgg
     4381 gttgcggtgg cagtgacggc cgtcggtgcg gtggtcgcgc tggcggtcaa gaacccggtg
     4441 cgtcagcccg tcacgcaggc gaatccaacg cccgagggtg agaaggcggc tcccgtcttc
     4501 gttctcgagt aggttccact cagcgcccat ccacaatgaa gcaatcccgg catccatgcg
     4561 atgccgggct tgcttcgttt gtgcctgcgg gtcgatctcg atcggagagg tcgccccggc
     4621 agggatgatc gtgccggata tcgggcggct gggtcagacc cgggtgatgc cgacgatcgc
     4681 gaccggtggt gtcagtgatt gaccctctga cgggctcgtc tggatcgcgc ggacgacatc
     4741 catgccctcg acgacctgac cgaaggccgc gaagccctgg ccgtcgggat tgcggaggcc
     4801 accaaagtcg agcgacggct ggtcgttgac gcagatgaag aagtccgaga tggcactgtc
     4861 cggcgtcgaa cgcgccatcg agatcgtgcc gtcgagatgg cggaggccgg tctcgctcgt
     4921 gcgctcgagt gcgatcggac cggcctgctc gccgatgcga tcgggattga cgccgccctg
     4981 gatcacctcg atcacgatcg aatcgttcgg cagctggcga cgatcggcgt tcggatcgat
     5041 cccgccgtcg gtatcgctga tccggagtcc ggcgttcgag ttgttgtccg tgcgaacggt
     5101 gcggtggaag cgaccgccgg tgtagaaccc accgtcgatg tagtggagga aattcgccgt
     5161 ggtgatcggc gtgtgctcgc cgtcgagggc gaccgtgatc gcgccgaccg gtgtctcgat
     5221 tcgaaccagc gtggaattcg tcatcttcga aggtatctcc aggttc
//
LOCUS       DASTMD010000207         3583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2875552, whole genome shotgun sequence.
ACCESSION   DASTMD010000207 DASTMD010000000
VERSION     DASTMD010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2875552"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFQ54_03110"
     CDS             complement(<1..438)
                     /locus_tag="VFQ54_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0164005.1"
                     /translation="MSASVVSPGATPATTSTAATAPVLALDNVHTYYGHIHALQGLTL
                     HVDAGEIVTLIGANGAGKSTTLKTISGLLSPRSGQITFEGRKINGMPAHKVAATGIAQ
                     SPEGRRVFPRMTVLENLEMGAFIHKRVDPTDLERVFSLFPRLKE"
     gene            complement(435..1322)
                     /locus_tag="VFQ54_03115"
     CDS             complement(435..1322)
                     /locus_tag="VFQ54_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0164006.1"
                     /translation="MVAIEPTPARYATPAEAEPVLVADNVTMRFGGLVAVDSVTMRVP
                     RGSISSVIGPNGAGKTTFFNMIAGIYKPTRGAILFEGRPLHRENRGPLGGSLRPDQIT
                     SLGISRTFQNIRLFSNMTAIENVLIGMHSRLKTNPVLAMVRTPGVRREELMAREKAYS
                     ILDFVGLGRFAEVTSKNLSYGDQRRLEIARALASEPRLLLLDEPTAGMNPRETAELTE
                     FIRGLRSSLDLTILLIEHDMKLVMGISDHVTVLDHGTKIAEGVPVEVQRNPQVVEAYL
                     GKGAAEILERGNKGAEVAS"
     gene            complement(1325..2566)
                     /locus_tag="VFQ54_03120"
     CDS             complement(1325..2566)
                     /locus_tag="VFQ54_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0164007.1"
                     /translation="MAIAVNSSGVSRSEAWFAARRTVERRRLVYLAIAVVVACLYPFA
                     DKAFGWNQLGAFVPMMVFVILAMGLNIVVGYAGLLDLGYVAFFIIGAYSSAFLTSPTS
                     YFVDKGWIPGFLQNFWAAMALSWLIAAVFGVLLGAPTLRLRGDYLAIVTLGFGEIVPA
                     FFTNAQSLTNGPQGIGQIATPPNIKLWGGHEIIFDATHQRNWFWLIIVVVVLSLWLIT
                     RLYDSRIGRAWQAIREDEIAAASMGINLTGTKLWAFGLGASFSGFAGAVYSGYVRVVY
                     PDQFQFTVSTTILAMVILGGLGNVYGVIIGALIIGFFDRILARELNSPLHWLGNHISD
                     AGASWLGNPITNHDVTTDKFLIFGLALVLVMLLRPGGLFPSRQRAAEMSGEDDGIVNG
                     NPANDFLEGPEPELGPLEGRG"
     gene            complement(2593..>3583)
                     /locus_tag="VFQ54_03125"
     CDS             complement(2593..>3583)
                     /locus_tag="VFQ54_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0164008.1"
                     /translation="MHIDWTLFTQFFIIGLATGSLIALIALGYTLVYGIIELINFAHG
                     EVFMMGAFFAATIAAASGVNSQSAPILIILVLALCLVGAMVFSGVLNVAIDTFAYRRL
                     RNAPRLAPLIAAIGVSFILQNIGIYWKGSRPFSGPNLIPQKYRTYNILKEWPILKDIF
                     GDTGLRLRVLDLFVIGVTVPLLIILSWFVFKTRIGTAMRATAHDREASALMGIDINRT
                     IATAFLLGGGLAGAAGMIALFYNNSGRFQMGFQYGLFAFTAAVLGGIGNLSGAVIGGY
                     IIGMIWSYSDVFMGEYISGWGSQWTSSVIFGILVIVLIFRPSGLFGTSTSEKV"
BASE COUNT          751 a         1101 c         1115 g          616 t
ORIGIN      
        1 ctccttcagg cgcggaaaga ggctgaagac gcgttcgaga tcggtcggat caacccgctt
       61 gtgaatgaag gcgcccattt ccagattctc gagcaccgtc atccggggga agacccgccg
      121 accctccggt gactgcgcga ttccggtcgc cgcgaccttg tgggcgggca tcccgttgat
      181 cttgcgcccc tcgaacgtga tctggcccga tcgcggcgag agaaggccgg agatcgtctt
      241 cagcgtcgta ctcttgcccg cgccgttggc cccgatcagc gtcacgatct cgccggcatc
      301 gacgtgcagc gtcagtccct ggagggcgtg gatgtggccg taataggtgt gaacattgtc
      361 cagtgccagc acgggcgcgg ttgcggccgt gctggtcgtc gccggggttg ctcccggcga
      421 cacaacggat gcgctcatga cgccacctcg gctcccttgt ttcctcgctc gagaatctcc
      481 gccgcgccct tgccgagata cgcctccacg acctgcgggt tccgctgtac ctcgaccggc
      541 accccctcgg cgatcttggt gccgtgatcg agaacggtca cgtgatcgga gatacccatc
      601 accagtttca tatcatgctc gatcagcagg atcgtcagat cgagactcga gcgaagaccg
      661 cgaatgaatt cggtcaactc cgccgtttcg cgcgggttca ttcccgcggt cggctcatcg
      721 aggaggagaa gccggggttc gctggccagg gcacgcgcga tctcgagtcg ccgctggtca
      781 ccataggaaa gattcttcga cgtcacctcg gcaaagcggc cgagcccgac aaagtcaaga
      841 atcgagtagg ccttctcgcg ggccatgagc tcttcccgac gcacgccggg cgtccttacc
      901 attgcaagca cgggattcgt cttcagccgg ctgtgcatgc cgatgagaac gttctcgatc
      961 gcagtcatgt tgctgaagag ccggatgttc tggaacgtgc gcgagatgcc gagtgacgtg
     1021 atctgatcgg gacgcaagga tccgccaagc gggccgcgat tctcacgatg cagcggtcgc
     1081 ccctcgaaaa ggatcgctcc ccgggtgggc ttgtagattc cggcgatcat gttgaagaag
     1141 gtcgtctttc ccgcgccgtt cggtccgatc acactcgaga tgctgcccct ggggacccgc
     1201 atcgtcaccg agtcaaccgc aaccagaccg ccgaaacgca tcgtcacgtt gtcggcgacc
     1261 agcaccggct ccgcttcggc gggtgttgcg taccgagcag gggtgggttc tatcgcaacc
     1321 atcgctaccc cctcccttcc agcgggccga gttctggctc aggtccctcg aggaaatcgt
     1381 ttgccggatt accgttgacg atcccgtcgt cctcgcccga catctccgcg gcccgttggc
     1441 gtgacgggaa gaggccaccg ggcctcagca gcatcaccag aaccagggcg agaccgaaga
     1501 tgaggaattt atcggtcgtg acatcatgat tcgtgatcgg attgccaagc cagcttgccc
     1561 cggcgtcgct gatgtggttg ccaagccagt gcaggggcga gttcagttcg cgggcgagaa
     1621 tgcgatcgaa gaagccgatg atcagcgcgc cgatgatcac gccgtagaca ttaccgagac
     1681 caccgaggat caccatcgca agaatcgtcg tcgagacggt gaactggaac tgatccgggt
     1741 agaccacccg cacgtacccg gaatagaccg cgccggcgaa tccggagaac gaagcaccga
     1801 gaccgaaggc ccagagcttg gttcccgtga gattgatccc catgctcgcg gcggcgatct
     1861 cgtcctcacg gatcgcctgc caggcgcgtc cgatccggga atcgtacagc cgggtgatca
     1921 gccagagcga aaggaccacg acgacgatga tcagccagaa ccagtttcgc tggtgggtcg
     1981 cgtcgaagat gatctcgtgc ccgccccaga gcttgatgtt gggcggcgtg gcgatctgcc
     2041 cgatcccctg cggaccgttc gtaagcgact gcgcattggt gaagaaggcg ggcacgatct
     2101 caccgaagcc gagcgtcacg atcgcgagat agtcaccccg gagccgaagc gtcggcgccc
     2161 cgagcagcac accgaagacc gcggcgatga gccaggagag cgccatggct gcccagaagt
     2221 tctggaggaa cccggggatc catcccttgt ccacgaagta gctggtcgga gatgtcagga
     2281 acgccgacga ataggcgccg atgatgaaga acgcgacgta gccgaggtcg aggagaccgg
     2341 cgtagccgac cacgatattg agacccattg ccaggatcac gaagaccatc atcggcacga
     2401 acgcgccgag ctggttccag ccgaaggcct tgtcggcgaa cggatagagg cacgccacca
     2461 cgaccgcgat cgccagatac acgagacgcc gtcgttcaac ggtgcgccgc gccgcgaacc
     2521 aggcttctga ccgggagacc cccgacgaat tcactgcgat tgccatcact caccccttcc
     2581 gggtccagga ccctagacct tctcggacgt tgacgtcccg aagagaccgg agggacggaa
     2641 gatcagaacg atgacgagga tgccgaagat gacggaactc gtccattggc ttccccagcc
     2701 gctgatgtat tcacccatga atacatcgct gtaggaccag atcatgccga tgatgtagcc
     2761 cccgatcacg gcgccgctga ggttcccgat gccgccgaga accgcggctg tgaaggcgaa
     2821 gagtccatac tggaatccca tctggaagcg cccggaattg ttgtagaaga gcgcgatcat
     2881 gcccgcggca ccggcaagcc cgccaccgag aaggaaggcg gtggcgatcg tgcgattgat
     2941 atcgatcccc atcaacgccg acgcctcgcg atcatgggct gtcgcccgca tcgccgtccc
     3001 gatccgcgtc ttgaagacga accaggagag gatgatcagg aggggaaccg tgacgccgat
     3061 cacgaagaga tcgagcacgc gcaggcggag tcccgtatcg ccgaagatgt ccttcaggat
     3121 cggccattcc ttgaggatgt tatacgtacg gtacttctgc ggaatgaggt tcggaccgga
     3181 aaacggacgc gagcccttcc agtagatgcc gatgttctgg aggatgaaac tgacgccgat
     3241 cgccgcgatc aggggcgcca gccgtggtgc gtttcgcagg cgcctgtatg caaacgtatc
     3301 gatcgccaca ttgaggacac cactgaagac catcgccccg acgagacaga gcgcaaggac
     3361 gagaatgatg aggatcggcg cggactggga gttcacaccg gatgccgccg cgattgtcgc
     3421 ggcgaagaac gcccccatca tgaatacttc gccatgcgcg aagttgataa gctcgatgat
     3481 gccgtagacg agggtatagc ccagcgcgat cagcgcgatg agggagcctg tggcaagacc
     3541 gatgatgaag aactgagtaa aaagcgtcca gtcgatatgc atg
//
LOCUS       DASTMD010000208         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3054868, whole genome shotgun sequence.
ACCESSION   DASTMD010000208 DASTMD010000000
VERSION     DASTMD010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3054868"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..487)
                     /locus_tag="VFQ54_03130"
     CDS             complement(26..487)
                     /locus_tag="VFQ54_03130"
                     /inference="COORDINATES: protein motif:HMM:NF042667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEU0164009.1"
                     /translation="MTTQSFDTADELRRMIGDGRISADALQAITGIQDETLASFLNGS
                     RSETIGLSRTPSSLSADESTRLSHLAAQLTEGFQIDDDVRLMAIIETLTAQCHLTHQN
                     IAALTRTGLDDLQGFLRDPDTLPLERKYQLAIRASYLINAVANAASASARH"
     gene            634..894
                     /locus_tag="VFQ54_03135"
     CDS             634..894
                     /locus_tag="VFQ54_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164010.1"
                     /translation="MTIIGVAVSVLTGMHGIPERFVWNATTYRVTDTPTRLDLDYATI
                     THPPAVPLGWRFQGTSDTGETRMFDVMFEPVRGEWLLLHTYE"
     gene            1391..>1542
                     /locus_tag="VFQ54_03140"
     CDS             1391..>1542
                     /locus_tag="VFQ54_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU0164011.1"
                     /translation="MALGTVKWFNAEKGFGFIAPDDGSPDVFAHYSAIATSGYKSLDE
                     NQKVEF"
BASE COUNT          279 a          461 c          500 g          302 t
ORIGIN      
        1 ggctgcggcc cgggcgaacg gccgctcagt gccgtgcgga agccgatgcg gcgttggcga
       61 cggcgttgat gaggtaggac gctctgatcg cgagctggta cttcctctcc aacggaaggg
      121 tgtcaggatc gcgcaggaag ccctgcaggt cgtcgagtcc ggtccgcgtc agcgcggcga
      181 tgttctgatg ggtcagatgg cattgggccg tcagcgtctc gatgatagcc atgagtcgca
      241 cgtcatcgtc gatctgaaac ccctccgtca gctgcgccgc gagatgcgac aggcgggtgc
      301 tctcgtcggc ggacagcgat gacggcgttc tggagagtcc gatcgtctcg gatcgagatc
      361 cgttcaggaa cgacgccaac gtctcgtcct gaatgcccgt gatggcctgc agggcgtcgg
      421 cggagatccg gccgtccccg atcatgcgtc tcagctcatc cgctgtatcg aacgactgtg
      481 tcgtcacgcc tgttcctccc tcatcaagat gcgtgaaccg cggtggctcg gtctcgctct
      541 gcccaactct gccccaccag ctttggcgtg tcgcggcggt tggtcgtaca gtctcagtct
      601 atcgaacaaa gattcgaatg tcgggaggcg gttatgacga tcatcggtgt ggccgtctcg
      661 gttctgacgg ggatgcacgg catcccggaa cgtttcgtct ggaacgccac cacctatcgg
      721 gtgaccgata ctccgactcg gctcgatctg gactatgcga cgatcacgca tccgcctgcg
      781 gttcctctcg gctggcgctt ccagggaacc agcgacacgg gcgagacgcg gatgttcgac
      841 gtgatgttcg agccggtgcg aggcgaatgg ctgctcctgc acacgtacga gtgacaccgc
      901 agaggtgcgg cggcagcgat caacccaggt cggcaacgat ctcggcgatg cgatgttcgt
      961 ggggctcggg cggcttcgca tccgacatct gcgtgacgaa caccccctgc gcgatggcga
     1021 gagcccttcg aacgccgcct gcctcccggc ggcctccaac gcggcagcga ggtccgacgg
     1081 tggacgagaa ggtggcgggc acgatggtcg cttcatctcc ggccgatttg ggggtctact
     1141 tccgttcgga gtccggctcg gcggcgaccg gcggagaccg tcgaaaacat tcatattgct
     1201 tttctgtcac acatggtcgc tttttccggc tttacggcta cgctagggcc tgcagttgca
     1261 gtacctcgca catcttgaat ggtgggaacc ccacggggag ccttgatctt tcgggagcgg
     1321 acgatgaaca ccgcgacgag cggatccgac ggtcggattt gcgcgaagaa actcctgaag
     1381 gagaaaaata atggcactag gaaccgtgaa gtggttcaac gctgaaaagg gctttggatt
     1441 catcgccccc gacgacggca gcccggatgt gttcgcacac tactcggcta tcgccacgag
     1501 cggctacaag tcgctcgacg agaaccagaa ggtcgaattc ga
//
LOCUS       DASTMD010000209         1691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2160900, whole genome shotgun sequence.
ACCESSION   DASTMD010000209 DASTMD010000000
VERSION     DASTMD010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2160900"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="VFQ54_03145"
     CDS             complement(<1..1321)
                     /locus_tag="VFQ54_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164012.1"
                     /translation="MGSKTSPDIAKQWSDWSISRRSLFKTSLAGVAAVAIANGGALPI
                     SSVAAQDDATPTAGGTLAMSLADDDVQSFDPIVPTDNMSIWTMLLIYDQLIRVASDGT
                     TLEPGLATTWDKSADGLTYTFHLRDAKFHDGTAVTADDIIYSLDRAAHDPTSTWASMV
                     TVIKSLAAPDPKTAVVTLSSIWAPFEADVALFACSIIPKAAHVAQKQALFDKPIGSGP
                     FVFDSWEKGSKITLKKNPSYWEPGKPYLDQLEFTVLTDANARMLQFQGGDLDVVTAVP
                     YSNIQSLQSNPDVVVLQDSVARIDYIGMNNARAPWNDKNLRQAINYAVDKDSLLQNVL
                     FGSGKAANTYLPLMAGHDDTIAGYPFDLDKAKELVAQSAGKDGWKGELIVGAGNTVGN
                     QIAQIIAAQMKQIGGDITLTTLEPAASRDRVRTKGDYDWTIGYYTTDI"
BASE COUNT          297 a          485 c          581 g          328 t
ORIGIN      
        1 tgatgtcggt cgtgtagtac ccgatcgtcc agtcgtagtc gcccttggtg cggacgcggt
       61 cgcggctggc ggcgggttcg agggtggtca gcgtgatgtc gccgccgatc tgcttcatct
      121 gggcggcgat gatctgcgcg atctggttgc cgaccgtgtt gccggcgccg acgatcagct
      181 cgcccttcca gccatccttg ccggccgact gggcgacgag ctccttcgcc ttgtcgagat
      241 cgaaggggta gccggcgatc gtgtcatcgt gaccggccat gaggggcagg taggtgttcg
      301 ccgccttgcc ggaaccgaag agcacgttct ggagcagcga atccttgtcg acggcgtagt
      361 tgatcgcctg gcgcaggttc ttgtcgttcc acggcgcgcg ggcgttgttc atgccgatgt
      421 agtcgatacg tgccaccgaa tcctgcagca cgacgacatc cggattcgac tgcagcgact
      481 ggatgttgct gtagggaacg gcggtcacga catcgagatc gccaccctgg aactggagca
      541 tgcgggcatt ggcatccgtc agcaccgtga actcgagctg gtcgaggtag ggcttgcccg
      601 gctcccagta gctcggattc ttcttgagcg tgatcttcga gcccttctcc cacgaatcga
      661 agacgaaggg gccggaaccg atcggcttgt cgaacagcgc ctgcttctgg gcgacgtggg
      721 ctgccttcgg gatgatcgag caggcgaaga gggcaacgtc cgcctcgaac ggcgcccaga
      781 tcgaactgag agtgacgacg gccgtcttcg gatccggcgc ggcgagcgac ttgatgacgg
      841 tcaccatcga cgcccaggtc gaggtcggat cgtgcgcggc acggtcgagg gagtaaatga
      901 tgtcgtccgc ggtgacggcg gtaccgtcgt ggaacttcgc gtcgcgaagg tggaaggtgt
      961 aggtcaggcc gtcggcgctc ttgtcccagg tcgtggcgag acccggctcg agggtggtgc
     1021 cgtccgacgc gacgcggatc aactgatcgt agatcagcag catcgtccag atcgacatgt
     1081 tgtccgtcgg cacgatcgga tcgaagctct ggacatcgtc gtcggcgagg ctcatcgcga
     1141 gcgtgccgcc ggcggtcggg gtggcgtcat cctgagccgc gacggaactg atcggcagtg
     1201 cgccgccgtt ggcgatcgcc accgcggcga cgccggcgag gcttgtcttg aagagcgaac
     1261 gacgcgagat gctccagtcg gaccattgct tcgcgatgtc gggtgacgtt ttcgagccca
     1321 tcgaatacct ccatgagaat gacgaatgat gaaccagacc gccgccggtt tcgcacgcgc
     1381 gcgaaaaccc gtggcgaccg atggatgtgt tcaccgggtc gcatctgtgc gcggagcgct
     1441 ggtgagatgc cccgaaagtc ccgcaacgtg tatcggcaag gatggtatgt cacgaccggg
     1501 tgagcaacaa gagtgtttcg gtgtgcagcg agcacaacat ggcagattac gtctcatgat
     1561 gacacgcggg acttgtgcaa tccgcccgta tgcctgccga tcgatctgtc acgaagcgat
     1621 ccgggatcgt cgcgagacgg gcgcggtgta caagtcgacg atcgtgtgat gtgatgccga
     1681 tacacgattt c
//
LOCUS       DASTMD010000210         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_555749, whole genome shotgun sequence.
ACCESSION   DASTMD010000210 DASTMD010000000
VERSION     DASTMD010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555749"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFQ54_03150"
     CDS             <1..189
                     /locus_tag="VFQ54_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164013.1"
                     /translation="IMSEFDCGKRVIYGHTPTREPLVLPNKIGLDTLPRSAGKLTVVA
                     LDDENPDAPPRFMFQPAF"
     gene            1277..1732
                     /locus_tag="VFQ54_03155"
     CDS             1277..1732
                     /locus_tag="VFQ54_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164014.1"
                     /translation="MPVRSLAKSGIPHWADEDSETASGKIERPRDIAARRFDRESRVV
                     VKSTAEAAASGRSRMPFSGPIRKAEATFAPFCDGLATRRAVPVGSCEIVLRTIGVDIS
                     AIRLAIATSSMRGPRFEDVRIGSLNLNHYFMHFPGNGVSLTLPEPGVTT"
     gene            1729..2025
                     /gene="rpsP"
                     /locus_tag="VFQ54_03160"
     CDS             1729..2025
                     /gene="rpsP"
                     /locus_tag="VFQ54_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU0164015.1"
                     /translation="MIKLRLRRMGAKKRPFYRIVAAEHSSPRDGRFIEIVGHYNPLTE
                     PATVVVKEDRARYWISVGAQPTETVAGLLTRAGVLADGKLVPAGEAAPVAEAKA"
     gene            2022..2384
                     /locus_tag="VFQ54_03165"
     CDS             2022..2384
                     /locus_tag="VFQ54_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU0164016.1"
                     /translation="MSARDDREAVDDTAVADEDDVELEGLTDGYQEDPSDSTIESLRG
                     LITFLVENLVDEPASAEIEVEQHGSAVQIRVNVPEEELGKIIGRGGRIAKAMRTALMI
                     AGSRHHLRVSLDIEGKPA"
BASE COUNT          479 a          729 c          796 g          490 t
ORIGIN      
        1 atcatgtcgg agttcgactg tgggaagcgc gtgatctacg gacacacgcc gacgcgtgag
       61 ccgctggtgt tgccgaacaa gatcggtctg gacacgcttc cgcggagcgc cggcaagctg
      121 accgtggtcg cgctggatga cgagaacccg gacgcgccgc cgcgattcat gttccagccg
      181 gcattctgag gcggttgatt ccggatccag gcggtcgtcc acagcgggcc gtcgtgaggc
      241 tcgctttccg cgtactgcgg tcacgcgcct cgcgcgttcc cgtcccggcg atgcgcgcga
      301 ccgcctcgta ttcgggaatc ctgcccgcca ttcgcgcgtt gaataggagg ttggggtccg
      361 cggcgagaga taaactcccg ccctacaaac ccctgtggtt cgaaatcatg tcttgcgcga
      421 ggtgattgcc cgaaaagccg tccgcgacgc ggtcgtgagt acggagggac ttctatgtcc
      481 cgcccgaggg cagtccgctg cggcggatcg gctccgccgg gagatttctc gaccgacagg
      541 ctcgaaatgg cgggatgggg ccgttggggg caggtgcgcg gaccgggaca cgcccggaat
      601 atgtgtgcag agaacgaggg aggggaggcc gatcggcctc ccctccctcc aaataggcga
      661 tttccggatt ttgatcttag tcagcgacgg aggcttgaga ctgcggcgtc atcatctggt
      721 ccggattaat gctcttcgat ttgcagcagg catgtacaac ggcagtagtc aaacattggg
      781 agaacccaag ttcccgcgca cggtcaacga tctcattggg tccctggcga tcgacgagag
      841 cctcggcgat aacgacgcta ggacggagat cccggatttc gggcttggga agagcggtca
      901 tgagggcggc attcctttct tttgcatgcg caatcaattc gtgaggtgtg catctgaccc
      961 gaatcccggc tcaaccaggc cgccgagaat ctgcggcgga cccacctgcg gatcgtctcc
     1021 aacttctcgt caagagcgag aagcaggaat caaatgcccc cctatcgtaa cgatccaacg
     1081 gtgcattagc aataggttga taatgcattt tgttgcaaat gattggcaac tcctgtgacc
     1141 agacgaccag agttgacgtc tttcgagcct cgcgaaggtg tacgaacacc tcgtttcgga
     1201 ttcgctcatc acggtcgcgc gtcttcgaac cttgtgacgt ttgggcacga agaccaatca
     1261 cgacagaagt cgaaggatgc cggtccggag cctcgcgaaa tcaggcatcc cccattgggc
     1321 ggacgaggat tcggaaactg catcgggaaa gatcgaacgg ccccgggata tcgcagcccg
     1381 tcgttttgac cgtgaatcgc gcgttgtggt aaagtcaacg gctgaagccg ccgcaagcgg
     1441 acgttctcgt atgccctttt ccgggccaat ccggaaagcg gaggcgacgt tcgcgccatt
     1501 ttgcgatggg ttggcgacgc gtcgtgcggt cccggtggga tcgtgcgaga tcgttttgcg
     1561 gacgatcggt gtggatatca gtgccatccg cctcgcgatc gcgacgtcat caatgcgtgg
     1621 tcctcgcttc gaggatgttc gaatcggctc gctgaatctg aatcactatt tcatgcattt
     1681 tccgggtaac ggcgtttcgt tgacgttgcc cgagccggga gttaccacgt gatcaagttg
     1741 cgactgcgcc gcatgggtgc caagaagcgc ccgttctacc gaatcgttgc cgccgagcac
     1801 agctccccgc gcgacggacg gttcatcgag atcgtcggtc actacaatcc gctcacggag
     1861 ccggcgacgg tcgtcgtcaa ggaagaccgc gcgcgctact ggatcagcgt tggcgcccag
     1921 ccgaccgaga cggttgccgg tctgctcacc cgcgccggtg ttctcgccga tggcaagctg
     1981 gttcctgccg gtgaggctgc tccggttgcc gaagccaagg catgagcgcc cgggacgatc
     2041 gcgaagcggt cgatgacacg gcggtggccg atgaggacga tgtcgaactc gaggggttga
     2101 cggacgggta tcaggaagat ccgagcgatt cgacgatcga atccctgcgt ggcctgatca
     2161 cctttctcgt cgagaacctg gtggacgagc ccgcgtccgc cgaaatcgag gtcgaacagc
     2221 acggttccgc cgtccagatc cgggtcaacg ttcccgaaga ggaactcggc aagatcattg
     2281 ggcgtggcgg ccggatcgcg aaagcgatgc ggacggcgct gatgatcgcc ggatcgcgtc
     2341 atcacttgcg ggtcagcctc gatatcgagg ggaaacccgc ctgatccttc ggcgagtaat
     2401 cccgtccatg gacagcgtgc ggaatgcctc accggcattc gcacgctgtt tcgcgtttcc
     2461 agcgcatgca gaacttcctc catgcggaag acag
//
LOCUS       DASTMD010000211         4243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2339807, whole genome shotgun sequence.
ACCESSION   DASTMD010000211 DASTMD010000000
VERSION     DASTMD010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339807"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..867
                     /gene="phnC"
                     /locus_tag="VFQ54_03170"
     CDS             70..867
                     /gene="phnC"
                     /locus_tag="VFQ54_03170"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEU0164017.1"
                     /translation="MLEINDLRVVYPNGYEALKSVTLKADDGEIVALIGRSGAGKSTI
                     LRCINGIQPVTSGRILLHGVDVTSMSGSELQLLRRRVGFIWQEHNLVERLTVMKNVLT
                     GRLGYINSMQSFFHWFGAEHRQIAVSSLERVNMTHRATQRADRLSGGEKQRVSIARAL
                     AQQPEIIVADEPVASLDVELAWQVMNDLVRVSRSEKVPTLISIHDVDLAKAFCDRIVG
                     LAQGEIVFDGPPSALDQVSMDLIYRFDKPIDLSAATGPTPIAIYGGA"
     gene            870..1754
                     /gene="phnE"
                     /locus_tag="VFQ54_03175"
     CDS             870..1754
                     /gene="phnE"
                     /locus_tag="VFQ54_03175"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEU0164018.1"
                     /translation="MASRSTPIPAVATATDKDMKRLLDPKPHIGFKAIGTIVVLVLVY
                     LWSIRGTDASPAELVRGWPYIWRFLSRLFPIQWQTTDWELGIGPWHWGTWTTTEVLPA
                     LFETIQMAIIGSTLSIIISLPFGLLAARNISPHWTVYQTMRFILNTIRAVPEIIIALI
                     FVAAVGLGPFAGVLALAIGSVGTTSKLYAEAIEAIDPTQVQAITATGANRLQTFMYAV
                     VPQALPLVASYSLLLFESNVRSASILGLVGAGGVGLILNTYMALFKYHELMGALLLLI
                     IAVTIIDRVSDYLRRRII"
     gene            1960..3048
                     /locus_tag="VFQ54_03180"
     CDS             1960..3048
                     /locus_tag="VFQ54_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0164019.1"
                     /translation="MKKFKMIPSAMMALVFAFTIPMFSTVAAQSATPEASPIASPAAT
                     PKVYDKTGWPKELDCGLFGGADAASALTGAQPIADYLESYLGIPMKYEVGTSYNAVIE
                     SMASNHTNCGTVGPFSYILAVQEAGAEALAVQVTPGTASGVTAVYDPTAAPSYYSVIS
                     VKKKSGIRTLPELKGKTLSFVDPASTSGYLIPSSELQSAGLDPKKDLKTVFAGDHPTS
                     VYALWNDKVDAAASTEDTLVNLAAEGQIDYCGFKDGIIGKVRTQDEINALWDSCPDGS
                     IVPILYSAPIPNTPFAVSKNLPESLKQAITDALVSIKDNPELVAKIGYWYVDPTQTDP
                     SLKNVDALYDPLREIAKQLNLDLKSFGN"
     gene            3210..>4243
                     /locus_tag="VFQ54_03185"
     CDS             3210..>4243
                     /locus_tag="VFQ54_03185"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF011990.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HEU0164020.1"
                     /translation="MSNLTVTNATVVLPDHILEETTVRIDDGIITAIETGSVSSLTEQ
                     ETIVDADGAYLIPGVVDLHNDNLEFEIHPRANANLPLHFALSTMERRLAGAGVTTEFH
                     AVSFQNMRGKGRTITDAEHKAEFLAAFDDDDRRAVRHNILHRLDVRTPTSIDSALPSL
                     RRVKRAYASLNDHTPGQGQYRDVDRLIKMAAESQAMRNAAPTEREWYLERMRQGLADT
                     EAVPAYYRHLQDELVHTPVILSTHDDDTIEKVDAQLALGATISEFPITFEAAERARAG
                     GMVILVGAPNIVRGGSQSGNLSAIELAAAGLADAIVADYHAPCLIASAFRLVNDGVRD
                     LPAAIAMITR"
BASE COUNT          767 a         1441 c         1249 g          786 t
ORIGIN      
        1 gcgaccactc ccgaatcacc ggatggtgcg gctccgtttc ctacgtcgtc tctcgtggag
       61 gtctaagtca tgctggagat caacgacctt cgggttgtct atcccaacgg gtacgaggcg
      121 ctgaagtccg tcacgctcaa ggcggatgac ggcgagatcg ttgcgctcat cggtcgctcc
      181 ggcgccggta aatcgacgat tctgcgctgt atcaacggca tccagccggt gacctcgggg
      241 cgaatcctgc tgcacggtgt cgatgtcacc tcgatgagtg gctcggaact ccagctgctg
      301 cgccgccggg tcgggttcat ctggcaggag cacaacctgg tcgagcgcct gacggtcatg
      361 aagaacgtgc tgaccggtcg cctcggctac atcaacagca tgcagagctt cttccactgg
      421 ttcggcgccg agcatcggca gatcgcggtc agcagcctgg agcgcgtgaa catgacgcac
      481 cgcgcgacgc agcgcgccga tcgcctctcc ggtggcgaga agcagcgcgt ctcgatcgcc
      541 cgcgccctcg cccagcagcc ggaaatcatc gtggcggacg agccggtggc atcgctcgat
      601 gtcgaactcg catggcaggt gatgaacgac ctcgtgcggg tctcccgctc cgagaaggtg
      661 ccgaccctga tcagcatcca cgatgtcgat ctggcgaaag ccttctgcga ccgcatcgtc
      721 ggtctcgccc agggcgagat cgtcttcgac gggccgccga gtgcgctcga ccaggtctcg
      781 atggatctga tctatcgctt cgacaagccg atcgaccttt ccgctgcgac cggcccgacg
      841 ccgatcgcta tctatggcgg agcgtagcca tggcctcccg ttcaacacct attcccgccg
      901 tcgccaccgc gacggacaag gacatgaagc gcctgctcga tccgaagccg catatcggat
      961 tcaaggcgat cggcaccatc gtcgtcctcg tgctcgtcta tctctggtcg atccggggaa
     1021 ccgacgcgtc accggcggag ctggtgcgcg gttggccgta catctggcgc ttcctctccc
     1081 gtctcttccc gatccagtgg cagacgaccg actgggaact cgggatcgga ccgtggcact
     1141 gggggacgtg gacgactacc gaagtcctgc cggcgctctt cgagacgatc cagatggcga
     1201 tcatcggatc gacgctctcg atcatcatct cgctgccgtt cggactcctc gccgcgcgca
     1261 acatctcccc gcactggact gtctaccaga cgatgcggtt catcctgaac accattcgcg
     1321 cggtgcctga aatcatcatc gccctcatct tcgtcgccgc ggttggtctc ggcccgttcg
     1381 ccggtgtcct cgcgctcgcg atcggctcgg ttggaacgac gtcgaagctc tatgcggagg
     1441 cgatcgaggc gatcgatccg acgcaggtgc aggcgatcac ggcaaccggg gcgaatcgcc
     1501 tccagacatt catgtacgcc gtcgtgcctc aggcacttcc cctcgtggcg tcctattccc
     1561 tgttgctctt cgagagcaac gtccgctcgg cgtcgatcct gggtctcgtt ggtgctggtg
     1621 gtgtcggact catcctcaac acctacatgg cgctcttcaa gtaccacgaa ctcatgggtg
     1681 cgcttctcct gctcattatc gcggttacca tcatcgatcg cgtcagcgat taccttcgaa
     1741 ggaggatcat ctagagcagc catccatcgc ctacccgtca cgagctaccc ggcatgatgg
     1801 atgtggtccg cagtgcgcaa cagctgtcca aagcccacgg ctgccggagg atcatccaga
     1861 accacgacgc gcaagaccta gttccgtgtt ctgctgctac cgccttggct tgttgtcaga
     1921 ggccagtgac tggccgttcc tttcgatcag gagtcttcga tgaagaaatt caagatgatc
     1981 ccttcggcga tgatggcgct cgttttcgcg ttcaccatcc cgatgttctc cacggttgcg
     2041 gcgcagtccg cgaccccgga agcttccccg attgcctccc cggcggcgac gccgaaggtg
     2101 tatgacaaga ccggctggcc gaaggagctc gattgcggtc tcttcggtgg cgccgacgct
     2161 gcttccgccc tcaccggcgc gcagccgatc gctgactacc tcgagagcta cctcggcatc
     2221 ccgatgaagt acgaagtcgg cacgagctac aacgccgtca tcgagtcgat ggcgtccaac
     2281 cacacgaact gcggcacggt tggcccgttc tcctacatcc tcgcggtgca ggaggctggt
     2341 gccgaggccc tcgccgtcca ggtcacgccg ggcaccgcct cgggcgtcac cgcggtctac
     2401 gacccgacgg ccgcgccgag ctactactcc gtgatctcgg tcaagaagaa gagcggcatc
     2461 cgcacgcttc ccgagctcaa gggcaagacc ctctccttcg tcgacccggc ctcgacctcc
     2521 ggttacctga tcccgtccag cgagcttcag agcgccggcc tcgatccgaa gaaggacctc
     2581 aagaccgtct tcgccggtga ccacccgacc tcggtttacg ccctctggaa tgacaaggtc
     2641 gacgcggctg cctccaccga ggacaccctc gtcaacctcg ccgccgaagg ccagatcgac
     2701 tactgcggtt tcaaggatgg catcatcggc aaggtccgca cccaggacga gatcaacgcc
     2761 ctctgggatt cctgcccgga tggctcgatc gtgccgatcc tctactcggc tccgatcccg
     2821 aacaccccgt tcgccgtttc caagaacctc ccggagagcc tgaagcaggc catcaccgat
     2881 gccctggttt cgatcaagga caatccggaa ctcgtcgcga agatcggcta ctggtacgtt
     2941 gacccgaccc agaccgaccc gagcctaaag aacgtcgatg cgctctacga tccgcttcgc
     3001 gagatcgcca agcagctgaa cctcgacctc aagtcctttg gtaactaggt agcagagcga
     3061 ccacaggatt cccggcaagg cgatcgcgcc agacctccct ctggtgcgat cgcccccggg
     3121 cgatacactg aatacacgaa cggcacgacg accggccatc gtacctggtg gccggtcgtc
     3181 gcgatcatga tcccggaggc agtctgtcca tgagcaatct caccgtcacc aacgcaacgg
     3241 tggtgctccc cgatcatatt cttgaagaga cgaccgtccg cattgacgat ggcatcatca
     3301 ccgccatcga gaccggttcc gtcagttcac tcacggagca ggagacgatc gtcgatgccg
     3361 acggcgccta tctgatcccc ggcgtggtcg atctccataa tgacaatctc gagttcgaga
     3421 tccatccccg cgccaacgcg aatctccccc tgcatttcgc gctcagcacg atggagcgcc
     3481 ggcttgcggg cgcgggtgtg acgacggagt tccatgccgt ctccttccag aacatgcggg
     3541 gcaaggggcg cacgatcacc gatgccgagc acaaggcgga attccttgcg gccttcgatg
     3601 atgacgaccg tcgcgcggtt cgtcacaaca tcctgcaccg gctcgacgtc cgcacgccca
     3661 cctcgatcga ttcggcgctg ccgtcgctgc gacgggtgaa gcgggcctat gcctcgctga
     3721 acgatcacac gccggggcag gggcagtacc gcgatgtcga ccgcctgatc aagatggcgg
     3781 cggaatcgca ggcgatgcgc aacgcggctc cgaccgagcg cgagtggtac ctggagcgca
     3841 tgcgccaggg gctcgccgac accgaggcgg tgccggccta ctatcgccac ctgcaggatg
     3901 aactcgtcca cacgccggtg atcctttcga cgcatgacga cgacacgatc gagaaggtcg
     3961 atgcccagct cgcgctgggg gcgacgatct ccgagttccc gatcaccttc gaggctgcgg
     4021 agcgggcgcg tgccggcggc atggtgatcc tcgtcggggc gccgaatatc gtgcgcggtg
     4081 gatcgcagag cggaaacctg agcgcgatcg aactggccgc ggcgggtctc gccgacgcca
     4141 tcgttgccga ttaccatgcg ccatgcctga tcgcctccgc cttccggctc gtgaatgacg
     4201 gcgtgcgcga cctcccggcc gcgatcgcga tgatcacgcg aaa
//
LOCUS       DASTMD010000212         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2339872, whole genome shotgun sequence.
ACCESSION   DASTMD010000212 DASTMD010000000
VERSION     DASTMD010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339872"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="VFQ54_03190"
     CDS             <1..1331
                     /locus_tag="VFQ54_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU0164021.1"
                     /translation="QRLMLDRYAGLEPLRAEVATGVQHWRDVKRRIVSLSTGAREREQ
                     RIDLLRFQIDEIAAAELQPGEDEALMRERDVLQNADRLRQDAELAMATISGDDAVTDV
                     PSVSSMLRAVEHAIRDLAEIDRNSLALSERATDLVVLAEDLARDLSGYLDDLPSDPER
                     LAAVDERLSLIQLLKRKYGATIEEISGFANAAAEELEGLTGNSFDIESLQSELATMEG
                     TLVDLTARLSSARQVAAVELAGRVETSIAELRMGRSELSIRVARRPDPDGIAVNGERV
                     HVDESGIDDVEFLIASSAGETLRPFARIASGGETARIMLALKSILSDVDATPTLVFDE
                     IDVGVGGRSGQVVGEKLWSITANHQVIVVTHLPQIAAFANHHLRIVKQEHGNRIVSVV
                     EEIRDDERIDELAAMLDGTPITPTSRSSAIEMLRRSEASRDAAGVPVRIP"
     gene            1449..1751
                     /locus_tag="VFQ54_03195"
     CDS             1449..1751
                     /locus_tag="VFQ54_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU0164022.1"
                     /translation="MQPNMKMIQQMQNRMAKIQQELEDTIIEGSAGGGVVKAEVTGQR
                     GLKSVKIDPSAVDPEDVEMLEDLVLAAIQDAMERAQKLAEDKMSALTGGIKIPGLT"
     gene            1773..2030
                     /locus_tag="VFQ54_03200"
     CDS             1773..2030
                     /locus_tag="VFQ54_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEU0164023.1"
                     /translation="MVLKPPLPIYMDDVVRLRKPHPCGSYDWVVVRIGADIGLRCLTC
                     GRRVLLPRRTVEKRLKTFLERGPEYPGDAGPEAIASPESAA"
     gene            2027..>3147
                     /locus_tag="VFQ54_03205"
     CDS             2027..>3147
                     /locus_tag="VFQ54_03205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164024.1"
                     /translation="MSRASTSANENGSENASIGAVLRNGPFLRLWLVQLTTQTCQNMI
                     NFALLLRVRDIVEFHHLPQANTAISLVILAFSLPAVLFGPVAGVIADRVNKRYLMSGL
                     NFARAIAVLLFLTIQTDWHVETILAANYLIVFLFGMAGQFFAPVEGATIPLVVKRQQL
                     LAANSLFSITNTAAQLVGFAAVGPILVKVLGIDSLFEITIVLYLVCSLIVLTLPKTPT
                     PPQREDLMHLGLMNRLWVDLKEGLLFILQDPMLMRAIGYLTIAATTFLMLAALAPAFV
                     TSVIGLSKEDIGYLVAPAGLGVLSGVFLVSRMVRWIGREWLIDLALTVAGILLFALAA
                     TRDALTWLLPGAPSTTLIAITCAVIAYCMGLCNAFILVPS"
BASE COUNT          577 a          998 c          995 g          577 t
ORIGIN      
        1 aacaacgact gatgctcgat cgctacgccg ggctcgagcc gttgcgcgcc gaggtcgcga
       61 caggcgtcca gcactggcgc gacgtaaagc gccggatcgt ctcgctttcc accggagctc
      121 gcgagcgcga gcaacggatc gatcttcttc gcttccagat cgacgagatc gcggcagcgg
      181 agctccagcc cggggaagac gaagcactga tgcgggaacg tgacgtcctg cagaacgcgg
      241 accggctgcg ccaggacgcc gaactcgcga tggcgacgat ctcgggagac gatgccgtga
      301 ccgatgttcc ctccgtgtcg tcaatgttgc gggccgtcga acacgccatt cgcgacctcg
      361 ccgagatcga tcgcaattcc ctggcgctct ccgagcgtgc caccgatctc gtcgtgctgg
      421 ccgaagacct cgcacgggac ctctccggct atctcgacga tcttcccagc gatcccgagc
      481 gattggcggc cgtggacgag cgactgagcc tgatccagtt gctgaagcga aagtatggcg
      541 cgacgatcga ggaaatctcc ggcttcgcga acgccgccgc ggaagaactc gaaggactga
      601 ccggaaactc attcgacatc gagtcgttgc agagcgagct tgccacgatg gaggggacac
      661 tcgtcgacct gaccgcccgc ctgtcgtcgg cgcgtcaggt agcggctgtc gaactggcgg
      721 ggcgggtgga gacgagcatc gccgaactgc gaatgggcag gtccgaactc tcgatccgcg
      781 tcgcgcgtcg tccagacccg gacggtatcg cggtcaacgg cgagcgggtg cacgtcgacg
      841 aatcgggtat cgacgacgtc gaattcctga tcgcctcgag tgccggcgag acgcttcgac
      901 cgttcgcgcg catcgcgtcc ggaggagaga cggcgcggat catgctcgcc ttgaagtcga
      961 ttctctccga cgtcgacgcg acacccacgc tcgtcttcga cgagatcgat gttggggtcg
     1021 gcgggcgctc cgggcaggtc gttggcgaga agctctggtc gatcacggcg aatcaccagg
     1081 tgatcgtcgt gacgcacctc ccgcagatcg ccgccttcgc caatcaccac ctgcggatcg
     1141 tcaagcagga gcatggcaat cgcatcgtct ccgtcgtcga ggagattcgg gatgacgagc
     1201 ggatcgatga gctcgcggcg atgcttgacg gcacgccgat caccccgaca tcgcgaagca
     1261 gcgcgatcga gatgttgcgc cggagcgagg cttcgcgtga cgcggcggga gttccggtca
     1321 gaatcccgta gtcacccgag atcgccgact atacttgcag cgcaccgcct atggcttaga
     1381 cgggaggggt gccccagcac ccctttctcg ggcggggcga ggattgaaac aaacaacagg
     1441 agcgatcaat gcagccaaac atgaagatga tccagcagat gcagaatcgc atggcgaaga
     1501 ttcagcagga gctggaggat acgatcatcg agggctccgc cggcggcggc gtggtcaagg
     1561 cggaagtaac cggtcagcgc ggcctcaagt ccgtgaagat cgatcccagc gccgtcgatc
     1621 ccgaagacgt ggagatgctc gaggacctcg tgctcgccgc gatccaggac gcgatggaac
     1681 gggcgcagaa gctcgcggaa gacaagatga gcgcgctgac gggcggcatc aagatcccgg
     1741 gactcacgta aatcgaatcg ggacatactg ccatggtgtt gaagccgcca ttgcccatct
     1801 acatggatga cgtggttcgt ctgcgcaagc cacacccctg tggatcctat gactgggttg
     1861 tcgtgcgcat aggcgcggat attggcttgc gttgccttac ctgcgggcgg cgggtgctgc
     1921 tgccccgccg gaccgtcgag aagcggctca agacgttcct ggagcgagga ccggaatatc
     1981 ccggtgacgc cggacccgaa gccatcgcaa gccccgagag tgcggcgtga gccgcgcatc
     2041 gacctccgca aacgagaacg gatccgaaaa tgcgtcgatt ggggccgttc tccggaacgg
     2101 cccgtttctg cgactctggc tcgttcaact caccacccag acctgccaga acatgatcaa
     2161 cttcgcgctc ctgcttcggg tgcgcgatat cgtcgaattt caccatctcc cccaggcgaa
     2221 cacggcgatc agtctggtga ttcttgcgtt ctcgctgccg gcggtgctct tcgggccggt
     2281 ggcgggcgtg atcgccgatc gcgtcaacaa gcgctacctg atgtccgggc tcaacttcgc
     2341 gcgggcgatc gccgtccttc tcttcctcac gatccagacc gactggcacg tcgagacgat
     2401 cctggcggcc aactacctga tcgtcttcct cttcgggatg gcgggtcagt tcttcgcgcc
     2461 cgtcgaggga gcaacgatcc cgctggtcgt caaacgacag cagctgcttg ccgcgaactc
     2521 cctcttcagc atcacgaata ccgcagcaca actcgttgga ttcgccgcgg tgggaccaat
     2581 tctggtcaag gtcctcggga tcgatagcct cttcgagatc acgatcgtgc tctatctcgt
     2641 ctgttcgctg atcgtgctga cgctcccgaa gacgccaacg ccgccccagc gcgaagatct
     2701 catgcatctt ggactgatga accgcctctg ggtcgatctc aaggagggac tgctcttcat
     2761 cctccaggac ccgatgctga tgcgtgcgat cggctacctg acgatcgctg ccacgacgtt
     2821 cctgatgctg gccgcgttgg cgcccgcctt cgtcaccagc gtgatcggat tgagcaagga
     2881 agacatcggc tatctcgtgg cgccggcggg actcggtgtc ctctcgggtg tcttccttgt
     2941 ctcgcggatg gtgcgctgga tcgggcggga gtggctgatc gacctcgcgt tgaccgtcgc
     3001 cggcatcctg ctcttcgccc tcgccgcgac gcgcgacgcg ctgacatggc ttctgcctgg
     3061 cgcgccgtcc acgaccctga ttgcgatcac gtgcgcggtg atcgcctact gcatggggct
     3121 atgcaacgcg ttcattctcg tgccgtc
//
LOCUS       DASTMD010000213         4081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1983344, whole genome shotgun sequence.
ACCESSION   DASTMD010000213 DASTMD010000000
VERSION     DASTMD010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983344"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1353
                     /locus_tag="VFQ54_03210"
     CDS             31..1353
                     /locus_tag="VFQ54_03210"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU0164025.1"
                     /translation="MNAQPEGVSELAPNATVKESDFHERATSVLEGTSQGSRLKRILP
                     FLGPAFIASIAYVDPGNFATNIQAGAKYGYMLVWVIVASNIMAILIQSLSAKLGIATG
                     HNLAEMCRERFPRPVVFGMWGIAELVAIATDIAEFIGAALGFNLIFGIPLFPAALLTA
                     VTTFLILGLQRYGARPLEAVISAMLAVIAVCYLIETVLEKPHWLGIGEAVVKPKFDGT
                     DSVLLAVGILGATVMPHVIFLHSALTQDRVRVRDPRLQRKLFRFELVDVIIALSIAGC
                     VNAAMLIMAATTFFNAGLSDIASIEDAHKTLEPLLGSGASLVFGISLLASGLASSTVG
                     TMAGQVIMQGFLRRHIPLWARRSITMVPSLFIIGLGLDPTNTLVVSQVVLSFGIPFAL
                     VPLVMFTRNPSIMGKALVNRQLTTAIASVVAALIISLNLFLLWSTIFD"
     gene            1432..2352
                     /locus_tag="VFQ54_03215"
     CDS             1432..2352
                     /locus_tag="VFQ54_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0164026.1"
                     /translation="MTQSSVLVPLDGSHLAEAVVPLAGRLATALSAKVMLLHVLEDDA
                     PAEVHGERHLSEAASARTYLEEVRAGLLAQGVAVVDIHVHENREHDVARAIVQHAREF
                     AVDLIALANHGRGGWREFFFGTIAQQVIRQEAAPVITIPVRSANEMAASAPLTLITMA
                     TNGSVEAEAALEPTVRMAKAFGVPVQVLMAVETLGTLSPDDRALASMVPSATRAVLDI
                     QAEQTQIYLDEVVDLLAERGVKAHGLMLRGDPADAIVQQATKDGSGLLVLATHGRSGL
                     SSIWTGSVGSKILARYGKPLLLVHAPDPES"
     gene            2453..3628
                     /gene="hemW"
                     /locus_tag="VFQ54_03220"
     CDS             2453..3628
                     /gene="hemW"
                     /locus_tag="VFQ54_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEU0164027.1"
                     /translation="MIVAPALPESPVGIYIHIPFCAHICPYCDFTTYAGKSAIIPRYV
                     EAVVRELELAGASHDGRSVATIFVGGGTPSLLTVAQMTAIIDACRASFQVEADAEVSI
                     EANPNGLTAELLAGYRAAGVNRLSIGAQTFDRRGLRTLGRHHEADDVLVALKAAQEAG
                     FDNVSLDFIFGWPGQTLDRWAEDLDRLITLGGDGLDHLSLYSLIVEPGTPFADAAARG
                     ILHIPDDDATADMYELAMTKLADAGWHHYEVSNWSRTPERFSRHNALYWRHGDYYGIG
                     AGAHGTVRGLRTMNQPLPETYCAEVEASRSPHSNSEVIDLVTAQGETMMVGLRLLREG
                     VNASAFAARYDATLDQIYGETIQDLIAIGMLERTDDGVRLTARGLMLANDVAARFLA"
BASE COUNT          707 a         1293 c         1322 g          759 t
ORIGIN      
        1 ccagcgcatc aacgtcgtcg gcaggtatgc atgaacgcac agccagaagg ggtgtcggag
       61 ctggcgccca acgcaaccgt taaagagtca gattttcacg aacgcgcaac gagcgttctc
      121 gagggaacga gccagggttc ccggctgaag cgaatccttc cttttctcgg ccctgcgttc
      181 atcgcaagca tcgcttatgt cgaccctggg aacttcgcga cgaacatcca agccggtgcg
      241 aaatacggct acatgctcgt ctgggtcatc gtcgcgtcga atatcatggc gatcctgatc
      301 cagtcacttt ccgcgaaact cggcattgcg actggacata acctcgcaga gatgtgccgg
      361 gagcgcttcc cgcgaccggt cgtcttcggc atgtggggta tcgcggagct cgtcgcgatc
      421 gcgacggata tcgccgagtt catcggcgcg gcgcttggct tcaacctgat cttcgggatc
      481 ccgctcttcc cggcagcatt gctcacggcc gtcaccacat tcctgatcct cgggctccaa
      541 cgctacggtg cccgtccgct ggaggccgtg atcagtgcga tgctcgccgt gatcgcggtc
      601 tgttacctga tcgagaccgt tctcgagaag ccgcattggc tcgggatcgg tgaggcggta
      661 gtgaagccga agttcgacgg gaccgacagc gtgctgcttg cggtcggcat cctcggcgcg
      721 accgtgatgc cgcatgtgat cttcctgcac tcggcgctga cgcaggatcg ggtccgggtc
      781 cgggatccac ggctgcaacg aaagctcttc cggttcgaac tggtcgatgt gatcatcgcc
      841 ctctcgatcg ccggatgcgt gaacgccgca atgctgatca tggccgcgac gaccttcttc
      901 aatgccggcc tgtccgatat cgccagcatc gaggatgcgc acaagacgct cgagccgctg
      961 ctcggctcgg gggcgagtct cgtcttcggg atctcgttgc tggcctcggg tctggcgtcc
     1021 tcgaccgtgg ggacgatggc gggccaggtg atcatgcagg ggttcctgcg gcgtcacatc
     1081 ccgctctggg cgcggcgttc gatcacgatg gtgccatcgc tcttcatcat cgggctcgga
     1141 ctcgatccaa cgaacaccct cgtggtgagc caggtcgtcc tttccttcgg cattccgttc
     1201 gccctggtcc cactcgtgat gttcacgcgc aatccgagca tcatgggcaa ggcactggtg
     1261 aaccggcagc tgaccacggc gatcgcctcc gtcgtcgccg cgcttatcat cagcttgaat
     1321 ctcttcctgc tctggagcac gatctttgac tagacgcatg gatgaatggt tccagtcgcg
     1381 tgagctgggc gcaatgcccg ggttggatct cggagaggga gtcggatcgt gatgacccag
     1441 tcctcggtac tcgttccact cgacggctcg catctggccg aagcggtggt gccgcttgcg
     1501 gggcgccttg caaccgcgct ctcggcgaag gtgatgctcc tccacgttct cgaggatgac
     1561 gcaccggcgg aggtgcacgg cgagcggcat ctttccgagg ccgcctcggc gcggacttac
     1621 ctcgaggaag tccgcgccgg ccttctcgcg cagggggtcg ccgtcgtcga catccacgtc
     1681 cacgagaatc gcgagcacga tgtcgcgcga gcgatcgtgc aacacgcgcg ggagttcgcg
     1741 gtggacctga tcgcgctcgc gaaccatggg cgcggtggtt ggcgggagtt cttctttgga
     1801 acgatcgcgc agcaggtgat ccgccaggag gcggcgccgg tgatcacgat tccggttcgc
     1861 tcggcgaacg aaatggcggc gagcgctccg ctcacgctga tcacgatggc gaccaacggc
     1921 agtgtcgagg ccgaggcggc gctggagccg acggttcgca tggcgaaggc gttcggcgtt
     1981 cccgtgcagg tcctgatggc ggtcgagacc ctcggcacgc tctcgccgga tgaccgcgcg
     2041 ctcgcgtcga tggtcccttc cgcgacgcgc gccgtgctgg acatccaggc cgagcagacg
     2101 cagatctatc tggacgaggt cgtggacctc ctcgcggagc ggggagtcaa ggcgcacggt
     2161 ctcatgctgc gcggcgatcc cgccgatgcg atcgtccagc aggcgacgaa ggacggttcc
     2221 gggcttctcg tgctcgcaac gcacggaagg agcggcttga gcagcatctg gacggggagc
     2281 gtcggctcga agatcctggc tcgctatggc aagccgctcc tgctcgtcca cgcgccggat
     2341 ccggaatcct gatccggccg ggccgggaat cagtccaccc gggtggacgt ttggctcagg
     2401 atcgcgcacg cttgtgggag tgtcagttct ctcctagcga atcctggaat ccatgatcgt
     2461 cgccccagca ctccccgaat cccccgtcgg catctacatt cacatcccgt tctgcgccca
     2521 catctgcccc tattgcgatt tcacgaccta cgcgggaaag agtgcgatca tcccgcgcta
     2581 cgttgaggcg gtggtccgcg aactcgagct cgccggcgcg tcccacgacg ggcgatccgt
     2641 ggcgacgatc ttcgtcggtg gcggcacccc gagcctcctg acggtggcgc agatgacggc
     2701 gatcatcgac gcctgccgcg cgtcattcca ggtcgaagcc gatgcggagg tctcgatcga
     2761 ggcgaatccg aacggcctga cggccgaact gctcgccggg tatcgcgccg ccggggtgaa
     2821 tcgcctgagt atcggcgccc agacattcga ccgacgtgga ctgcgcaccc tcggtcgcca
     2881 tcatgaggcg gacgacgtcc tcgtcgcgct gaaggcggcg caggaagccg gtttcgacaa
     2941 cgtcagtctc gacttcatct tcggatggcc gggccagacc ctcgatcggt gggcggagga
     3001 tctcgatcgg ttgatcacgc tcggtggtga cgggctggac catctctcgc tctacagcct
     3061 gatcgtcgaa ccggggactc cgttcgccga tgcggcggca cgggggattc tccatatccc
     3121 ggacgatgat gcgaccgccg acatgtacga actggcgatg acgaagctgg cggatgccgg
     3181 ctggcaccac tacgaggtct cgaactggtc gcgcacacca gagcgttttt ctcgacataa
     3241 cgcgctgtac tggcggcacg gtgactacta cggcatcggc gccggcgcgc acggcacggt
     3301 ccggggtctc cgcacgatga accagccgct accggagaca tactgcgccg aggtcgaggc
     3361 gagtcgttcg ccacattcga actcggaggt catcgacctc gtgacggcac aaggggagac
     3421 gatgatggtc ggcttgcgac tgctccggga gggggtgaat gcgagcgcct tcgcagctcg
     3481 ctacgatgcc accctggatc agatctacgg agagacgatc caggatctga tcgcgatcgg
     3541 gatgctcgaa cggaccgacg acggcgtccg cctcaccgcg cggggactga tgctcgcgaa
     3601 cgacgtcgcg gcgcgattcc tggcgtagag cgagcggaaa gcccttcgca ggaacacacg
     3661 gccccgcggg gtattctgtg cagtcggggt ctgcaagcag ttccagaagg cgggatatag
     3721 aagtccctcc ctacggacga ccgattctcc gccgtagcga gtcaccccat gtgacccatc
     3781 gggcgcagcc cgatatcccg cccgagaagt gaatgccatc gcacccagga tttggtggat
     3841 gcgccaatag agcgaggttc agggcgatcg caacaccggt ttcacgcgcc gcgcaggtga
     3901 tctcgtttgc ggcaagacct tgtgtcttgc cctacaaacc cgggtaatcc gaagagacct
     3961 gaagcgcgcg cgagttcatc tctcgccgcg aactcaacat caatgcaaag cgccttgcat
     4021 caaggggaga agggcgagca cgaggctcgc ccctacggga tcatggtaat gcggctttgg
     4081 g
//
LOCUS       DASTMD010000214         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_199154, whole genome shotgun sequence.
ACCESSION   DASTMD010000214 DASTMD010000000
VERSION     DASTMD010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199154"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /locus_tag="VFQ54_03225"
     CDS             complement(<1..1788)
                     /locus_tag="VFQ54_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU0164028.1"
                     /translation="MIASRSLTTTLKDVGIETSIVVRHLIVWMVEALVLIGLAKWIPG
                     IRITGFGDALVVILALATINAFVMPGLFRIAVHMRAFLFPIATFFLDGSTLVALDSLL
                     PNWDVDGWLVSGVIAGILTIVASVLGSILALSDEQAWQRYSLGPMRSRYLRGQATIPQ
                     AHGFIFLEIDGLAEPVLRDAIARGYVPNMASWLRSGSHCLARWECDLSSQTSASQAGI
                     LLGNNANVPAFRWYDKELRRVIVSNHFRDSALLQERLSSGDGLLRDNGASRGNLFSGD
                     APDSLFTFSTLGDRGQGGNNKYWFFYANLFNIARTLALFVADIFRELAANLWQQLRNE
                     RPRVHRGGVYPFARAATTSLLREFSTFTVAGDMMRGVASVYTTYLAYDEVAHHSGIAR
                     GDTLRVLKAVDRDIGRLARVAADTTRPYHLVVLSDHGQSQGATFRQEYGVTLRELVEQ
                     TIAGYPGHHAGEAVIGNGNGGTDEGWQGLSALLTDVLVRDRQAHPMMQRAFRRKITDG
                     EVHLGYQEREFAADAGVVVLASGSLGLISFPGWAERVTLEEIEVEFPALIPALVEHPG
                     IGFIVVATKENGPVVLGSKGVYILDEDRAV"
     gene            complement(2168..2650)
                     /locus_tag="VFQ54_03230"
     CDS             complement(2168..2650)
                     /locus_tag="VFQ54_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0164029.1"
                     /translation="MNQADVLAVLNDPVSRRLIDSSNPVRLAYTGKDGFPRVVPLAFH
                     WNGSQFVIGTIPGSAKTGTLAVNPKVAMTIYTTAFPPNVLLVRGTASLELVDGVPDEY
                     IEANRKQIPAGMFPEWQNGVRHLYKEMVLIKVTPEWAKVMDFETRLPRSVEKAVRAAF
                     "
BASE COUNT          516 a          911 c          941 g          482 t
ORIGIN      
        1 gaccgcgcga tcctcgtcga ggatgtagac acccttgctg cccagcacga ctggtccgtt
       61 ctctttcgtc gcgacgacga tgaagccgat gcccgggtgt tcgacgagtg ccgggatcag
      121 ggcaggaaat tcgacttcga tttcctcgag cgtcacccgc tccgcccacc cggggaagga
      181 gatcagcccc agactgcctg atgccagaac gacgacaccg gcatcagccg caaactcccg
      241 ttcctgatag ccgagatgca cctcgccgtc ggtgatcttt cgccggaagg cacgctgcat
      301 catcgggtgc gcctggcgat cgcgtacgag cacgtccgtc agcagcgcgc tcagtccctg
      361 ccacccttcg tccgtgcctc cgttgccatt gccgatcacg gcctccccgg cgtggtggcc
      421 gggatagccg gcgatcgtct gctcgacgag ctcgcgcaag gtcacgccgt attcctgccg
      481 gaaggtcgcg ccctggctct gcccgtggtc cgagagcacg accaggtgat atgggcgcgt
      541 cgtgtccgct gcgacacgcg ccagccgccc gatatcgcga tcgaccgcct tcaggacccg
      601 cagcgtatcc ccgcgcgcga taccggagtg atgcgcgacc tcatcgtagg caaggtaggt
      661 cgtgtagacg gacgccacgc cgcgcatcat atcgccggcc acggtgaacg tgctgaattc
      721 gcggaggaga cttgtcgtcg cggcgcgcgc gaacgggtag acaccacccc ggtgaaccct
      781 tggccgctcg ttgcgcagtt gctgccacag attcgccgcc agttcccgaa agatgtcggc
      841 gacgaagagc gcgagcgtac gggcgatgtt gaagaggttc gcgtagaaga accagtactt
      901 gttgttgccg ccctgcccac gatcgcccag cgtcgagaag gtgaagaggc tgtccggcgc
      961 atccccggag aagagattgc cgcggctcgc cccgttgtcg cggagaagcc cgtcgccact
     1021 cgagagccgc tcctggagga gcgcgctgtc ccggaagtgg ttcgagacga tcactcggcg
     1081 aagctccttg tcataccagc gaaaggcggg aacgttggca ttgttgccga ggagaatccc
     1141 ggcctggctg gccgaggtct gcgaggagag gtcgcattcc caccgcgcga gacaatggga
     1201 gccacttcgc agccaactcg ccatgttcgg gacgtacccg cgtgcgatcg cgtcccggag
     1261 cacgggctcg gccaggccgt cgatctcgag gaagatgaat ccgtgcgcct gcggaatcgt
     1321 cgcctggccg cggagatagc gcgaccgcat cggaccaagc gagtagcgtt gccaggcctg
     1381 ttcgtcgctg agcgcgagga tgctgccgag cacactcgcg acgatggtca ggatgccggc
     1441 gatgacgccg gagacgagcc agccatcgac gtcccagttc gggaggaggc tgtcgagcgc
     1501 gacgagggtg ctgccgtcga ggaagaaggt ggcaatcggg aagaggaacg cgcgcatgtg
     1561 gacggcgatc cggaagagac ccggcatgac gaaggcgttg atcgtcgcga gggcgaggat
     1621 gacgacgagc gcatcgccga agccggtgat ccggatcccg ggaatccact tggcgagacc
     1681 gatgaggacg agggcttcga ccatccagac gatcagatgc cggacgacga tgctcgtctc
     1741 gatcccgacg tctttcaggg tggtggtcag cgagcgcgat gcgatcatct gggcaggata
     1801 agggatcgca cgacccgcga ggtaggtcgg ggccgtccat cacccgcatc ccttcccgct
     1861 ccccgacgcg cacttcctgt cctcacggcc cgccaacccc gtcctcacgg cccactaacc
     1921 ccgtccccgc gacccatgtg gtcgcccggg accacatccc attcgcgatg cctcgcctgc
     1981 tcgccccacg tccggcaacc gtgtcgcgcc gggagcacgc ggatgccggc tggtccctcg
     2041 acaggctcgg gaggacgtgt tgtgtacggc gccgtgaggg gagcacgcgg atgccggctg
     2101 gtccctcgac aggttcggga gggcgtgttt gttgatgcgg ggtgatgggt ggggtcgggg
     2161 tggcgaatca gaatgcggcg cgcacggcct tctcgaccga gcgcggcagg cgtgtctcga
     2221 aatccatcac cttcgcccac tcgggcgtca ccttgatcag caccatctcc ttgtagaggt
     2281 gccttacccc gttttgccac tcggggaaca tcccggcagg aatctgcttc cggttcgcct
     2341 cgatgtattc atccgggaca ccatccacca attcgagact ggccgttccc cgcacgagca
     2401 ggacgttcgg ggggaatgcc gtcgtgtaga tcgtcatggc cactttcggg ttgaccgcga
     2461 gcgtccccgt cttcgccgaa ccggggatcg tcccgatcac gaactgcgaa ccgttccagt
     2521 ggaacgcgag cggcaccacg cgcggaaacc catccttgcc ggtgtatgcg aggcgaaccg
     2581 gattgctgga atcgatcagg cgccgggaaa cgggatcgtt gagaacggcg agaacatcag
     2641 cctggttcat gaagagttcc tttcggcgag cgagtgcaat cccaccggat gaccgcggcg
     2701 gcacggatga cagaggaccc ggcgcgacga tggttcacgc cacatcgtat tcctgccgtg
     2761 ggaaagatgt ttcttcacga ttgcggtctg aggcgaaaat tcgaaagggg ggaatgcgga
     2821 actcctgcgc atttcgcggc gcacagggat
//
LOCUS       DASTMD010000215         1786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2965745, whole genome shotgun sequence.
ACCESSION   DASTMD010000215 DASTMD010000000
VERSION     DASTMD010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2965745"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFQ54_03235"
     CDS             <1..453
                     /locus_tag="VFQ54_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164030.1"
                     /translation="AARFDVEEDPQQTQPLTGGIPDREPPSPWALNGSSSARWSPDST
                     ETAYTSPINATSVSTPPIATRFDVDSTAEAMNPGRTDVWAADYDGPSPGIVTDQGEYA
                     IEEESLRGFFLGVTAIVLIIIVAAIYGWTITPDGSFRDTIQGWINDIS"
     gene            507..>1786
                     /locus_tag="VFQ54_03240"
     CDS             507..>1786
                     /locus_tag="VFQ54_03240"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU0164031.1"
                     /translation="MLSVSTTVGFPNQSIAATHSWERRRPFPTYATTGMIGAAHPLTV
                     EAGLYILRSGGNAIDAAVGAGLVAAVVMPEMCGLGGDLFAVVHRPNPTGGKGDVVSVQ
                     GSGISPRSASIEHMRKHGREDGLKMPYRGALAIGVPGMVDAYFKLLEKYGTKSFAEIA
                     EPAIAHARDGFAVMPHGAASLAGTAPELANDPAAAAVFLAGGKPVGAGEKLVQADLAR
                     TLEELGRDGVDSFYRGAIARRILDYLTSVGGLFTAEEFAEHTTDFTDPVATTYRGQTV
                     YQTAIPSQGLILLESLNIIEQGEIDPDLSPETIHLLIEAKKRAYADRLAYTADPNFFD
                     SPIETLLSKPWAASRYASIDLQQASTNVPAGEYNDGDTTYLNVVDKDGMMVSLIQSVS
                     SSFGSCVVGGDTGVVLNNRVGRGFSLVDGHPNIYA"
BASE COUNT          302 a          618 c          551 g          315 t
ORIGIN      
        1 gccgctcgct tcgacgtcga ggaggatccc cagcagacgc aaccactgac gggcggcatt
       61 ccggatcggg agccgccgtc accgtgggcg ctgaatggct cgtcctccgc ccgctggtcg
      121 cctgattcga ccgagaccgc atatacctca ccgatcaacg cgacgtcggt ttcgaccccg
      181 ccgatcgcca cccggttcga cgtcgattcc acggcggagg cgatgaatcc cggccgtacc
      241 gacgtctggg cggcggacta cgatggtcca tctcccggaa tcgtgacgga tcagggagaa
      301 tatgccatcg aggaggagag tcttcgcgga ttcttcctgg gtgtcaccgc catcgtgctg
      361 atcatcatcg tcgccgcgat ctacggctgg acgatcactc cggatgggag cttcagggat
      421 accatccagg gctggatcaa cgacatctcc tgaccagtcg tgcttcctca ctcgtcgtct
      481 atccgtttca ggacaggagt tcccaggtgc tttccgtctc cacaaccgtc ggctttccga
      541 accagtccat cgcggctacg cattcctggg agcgccgccg tccgtttccg acgtatgcga
      601 ccacgggcat gatcggcgca gcgcacccgc tgacggtcga agccggtctt tacatcctcc
      661 gatccggcgg caacgcgatc gacgcggcgg ttggcgccgg tctcgtcgcg gccgtcgtga
      721 tgccggagat gtgcggtctc ggcggcgatc tcttcgccgt cgtccatcga ccgaatccga
      781 ccgggggcaa gggtgacgtc gtctcggtcc agggcagcgg catctcgcca cgcagcgcgt
      841 cgatcgagca catgcgcaag cacggccgcg aggacgggct gaagatgccg taccgcggag
      901 cgctcgcgat cggcgttccg ggcatggtcg acgcctactt caagctgctc gagaagtacg
      961 gaaccaaatc cttcgccgag atcgccgaac ccgcgatcgc ccacgcgcgg gacggcttcg
     1021 cggtgatgcc gcacggcgcc gcctcgctgg cgggaaccgc accggaactc gccaatgacc
     1081 ccgcggcggc ggcggtcttc ctggcgggcg gcaagccggt cggcgccggc gagaagctcg
     1141 tgcaggccga tctcgcccgc accctcgagg agcttggtcg cgacggcgtc gacagcttct
     1201 accgcggcgc gatcgccagg cgcatcctcg actacctgac ctcggtcggt ggtctcttca
     1261 cggcagagga attcgccgag catacgacgg atttcaccga tcccgtcgcc acgacctatc
     1321 gtggccagac ggtctatcag acggcgatcc cgagccaggg cctgatcctt ctcgagagtc
     1381 tgaacatcat cgagcagggc gagatcgatc ccgatctctc gccggagacg atccacctcc
     1441 tgatcgaggc caagaagcgc gcctatgccg atcgcctcgc ctacacggcc gatccgaact
     1501 tcttcgattc gccgatcgag accctgctct cgaagccgtg ggcagcgtcc cgctacgcct
     1561 cgatcgacct ccagcaggcg tcgaccaatg ttccggccgg tgagtacaac gacggggaca
     1621 cgacctacct gaacgtcgtc gacaaggacg gcatgatggt ctcgttgatc cagtccgtct
     1681 cgtcgtcgtt cggcagctgc gtcgttggcg gcgacaccgg cgtggtcctg aacaaccgcg
     1741 ttggccgcgg cttctcgctc gtcgacggcc atcccaacat ctacgc
//
LOCUS       DASTMD010000216         1980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2609400, whole genome shotgun sequence.
ACCESSION   DASTMD010000216 DASTMD010000000
VERSION     DASTMD010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2609400"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFQ54_03245"
     CDS             <1..605
                     /locus_tag="VFQ54_03245"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEU0164032.1"
                     /translation="WHAVVGGFAVVGFALVHAGAFLALKTDGAVRQRARRFVVTFASV
                     LLVPIVIWVFAVLATDGNRYTWPLIAVAAIAASVAWLRMRAGHEGQAFAGMAIFLIAG
                     AAAIFGSVYPVVLPSTLNPAWNLTIDSASSSPYTLKLMSIIAAFGVPLVLVYQGWTYW
                     VFRQRISEHHIPDAHTVPVAVAPPSPTPTIRTHTQAGQPL"
     gene            602..>1980
                     /locus_tag="VFQ54_03250"
     CDS             602..>1980
                     /locus_tag="VFQ54_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEU0164033.1"
                     /translation="MKAHASRSPFANLPASARRTLPLLALLSAGKAIALVCLAGALAS
                     GIVSAIDGTDRWKVDLLWGIGAGVTRAIIAWATPVVANRAALGAKEELRAQLAERAAV
                     RGGRDLPQGVGELTTLATRGLDDLDPWFTQYLPALMSATTIPLLVGVRILTADWISAL
                     IVALTIPLIPVFMILIGWQTQERMANAADALARLSDHLVELARGLPVLVGLGRADAQT
                     RTLRDVSGRYREKTMATLRIAFLSSLALELIATISVALVAVVIGVRLVHGDLGLDVAL
                     LVLILAPECYLPFREVGAAHHASEDGIEALQRAKAVIATPVSQPLLREIRPVAFGLAV
                     EDLTVQYADRSRPAVERLNFRANPGQIVALVGPSGAGKSTALAAIAGLIGDGGESGAR
                     VEGRIDGVDPAKLAWVPQHPVTYAESVREEIALACDIPRADIIDTVLRRVDAGNLADR
                     HPGELSPGEL"
BASE COUNT          285 a          681 c          657 g          357 t
ORIGIN      
        1 actggcatgc cgtcgtcggt ggatttgccg tcgtcggctt cgcgctcgtc cacgctggcg
       61 cgttcctcgc gctgaagacg gacggcgcgg ttcgccaacg ggcccggagg ttcgtcgtga
      121 ccttcgcgtc ggttcttctc gtgccgatcg tgatctgggt attcgccgtg ctggccacgg
      181 atggcaaccg ctacacgtgg ccgctgatcg cggtcgcggc gatcgccgca agcgtcgcct
      241 ggctccggat gcgcgccggc catgagggcc aggcgttcgc cgggatggcg atcttcctga
      301 ttgccggagc cgccgcgatc ttcggttccg tctacccggt cgtcctgccc tcgacgctga
      361 acccggcctg gaacctcacg atcgactcgg catcttcctc cccctacaca ttgaagctga
      421 tgagcatcat cgcggcgttc ggggtgccgc tcgttctcgt ctaccaggga tggacctact
      481 gggtcttccg ccagcggatc agcgagcatc acattcccga cgcccatacc gttcccgtgg
      541 ccgtcgcgcc gccatcgcca acccccacca tccgcacaca cacccaagca ggtcaaccgc
      601 tgtgaaagct cacgcatccc gatccccctt tgcgaatctg ccggcgagcg cacgccggac
      661 cttgccattg ctcgcgctcc tgagcgccgg gaaggcgatc gcactcgtct gcctggcggg
      721 agcactggcg tccggtatcg tcagcgcgat tgatggaacc gatcgctgga aggtcgatct
      781 cctctggggg attggtgcgg gagtcacgcg ggcgatcatc gcctgggcga ctcccgtcgt
      841 cgcaaaccgg gccgcgctcg gggcgaagga agaactccgc gcccagctgg cggaacgggc
      901 cgcggttcgc gggggacgcg atctgccgca gggggtcggc gaactgacga ccctcgcgac
      961 gcgtggcctc gacgatctcg atccctggtt cacgcagtac ctgccggcgc tgatgagcgc
     1021 gacgacgatc ccgctgctgg tcggtgtccg catcctcacg gcggactgga tcagcgccct
     1081 gatcgtcgcc ctgacgatcc ccctgatccc cgtcttcatg atcctgatcg gctggcagac
     1141 gcaggagcga atggccaatg ccgccgatgc tctcgctcgc ctctccgatc acctcgtcga
     1201 actggcgcgt ggattgccgg tcctcgtcgg gctcggccgg gcggacgccc agacgcggac
     1261 gctgcgtgat gtctccgggc gctaccgcga gaagacgatg gcgacgctgc ggatcgcctt
     1321 cctctcctca ctcgcgctgg aactgatcgc gacgatctcg gtggcgctcg tcgccgtcgt
     1381 catcggcgta cgcctcgtgc acggcgatct cggtctcgat gtcgcactgc tcgtcctgat
     1441 cctggcaccg gagtgctatc tgccgttccg ggaggtcggg gctgcgcatc acgcctccga
     1501 ggatgggatc gaggcgttgc agcgggcaaa ggccgtgatc gcgacgccgg tgtcgcagcc
     1561 gttgctccgg gaaatccgac cagtcgcgtt cgggcttgcc gtggaggacc tgaccgttca
     1621 gtatgccgat cgttcgcggc cggcggtcga gcgcctgaac ttccgggcga atccgggtca
     1681 gatcgtcgcc ctcgtcggtc ccagcggcgc cggaaaatcg acggccctcg ccgcgatcgc
     1741 gggactgatc ggagacggtg gagagagcgg agcgcgtgtc gagggacgga tcgacggcgt
     1801 cgacccggcg aagctcgcct gggttccgca gcatcccgtc acctatgccg aaagcgtgcg
     1861 tgaggagatc gcgctggcgt gcgacatccc gcgcgccgac atcatcgaca cggtgctgcg
     1921 gcgcgtggat gccggaaacc tcgcggatcg tcatcccggc gaattgagtc cgggcgaact
//
LOCUS       DASTMD010000217         1551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1983667, whole genome shotgun sequence.
ACCESSION   DASTMD010000217 DASTMD010000000
VERSION     DASTMD010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983667"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VFQ54_03255"
     CDS             <1..851
                     /locus_tag="VFQ54_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164034.1"
                     /translation="KPPYGYRDWWPIGLGFNCMKAPFDDANIRWAINYAIDRDKLIQF
                     AYKGAGEGSILPFPKFPALVAYTDEIQDLQDQLNEHGQDQVDAKMTGAGWAKNGDGKW
                     AKDGKTFDMVILSQTLFQDVAPILSQQLQDAGFNASFKNTIGNEFSDSVYTGNMDAFI
                     YGHGGGVRDPYFTLNLYHSRYSLPTGQRAQQPYRWVNADYDKLVEQMALAEEGSQEVH
                     DLFKQAMAIWVKDLPDVQLAQWFHRIPMNSTYWTNLPNEDNPYINSAFWHRCSPLWIN
                     TIKPAK"
     gene            1114..>1551
                     /locus_tag="VFQ54_03260"
     CDS             1114..>1551
                     /locus_tag="VFQ54_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164035.1"
                     /translation="MPHKPGQSLFLTRVDDPDLTRIRSDPASTRCPPHAERVGLRMPT
                     SLPPNGLVLWPDHISSPVPTEEETTVTDAVPTTPDMTVADHHRPQYHVTAPQNWLNDP
                     NGLIQWRGIYHMFYQYTPDNPLGGVKVWGHAISHDLVNWEHLPV"
BASE COUNT          311 a          535 c          448 g          257 t
ORIGIN      
        1 acaagccgcc ctacggctat cgtgactggt ggccgatcgg tctcggcttc aactgcatga
       61 aggcgccgtt cgacgatgcg aacatccgct gggcgatcaa ctacgcgatc gaccgcgaca
      121 agctgatcca gttcgcctac aagggcgccg gcgaggggtc gatcctgccg ttcccgaagt
      181 tccccgccct cgtcgcctat accgacgaga tccaggatct tcaggaccag ctcaacgagc
      241 acggccagga ccaggtcgat gccaagatga cgggcgccgg ctgggcgaag aatggcgatg
      301 gcaagtgggc taaggatggc aagaccttcg acatggtcat cctcagccag accctcttcc
      361 aggacgtcgc gccgatcctc tcgcagcagc tgcaggatgc cggattcaac gcctcgttca
      421 agaacacgat cggcaacgag ttctcggaca gcgtctacac cggcaacatg gacgccttca
      481 tctacggtca tggcggtggc gttcgcgatc cctacttcac gctgaacctc taccacagcc
      541 gctattcgct gccgacgggt caacgcgcac agcagcctta ccgctgggtg aacgccgact
      601 atgacaagct cgtcgagcag atggcgctcg ccgaggaggg atcgcaggag gtgcacgatc
      661 tcttcaagca ggcaatggcg atctgggtca aggacctccc ggacgtccag ctggcgcagt
      721 ggttccaccg gatcccgatg aactcgacgt actggaccaa cctcccgaac gaggacaatc
      781 cgtacatcaa cagcgcgttc tggcatcgct gttcgccgct ctggatcaac acgatcaagc
      841 cggcgaagta gtcgattccg ggcggtcgaa acgaaaacga gggcgcgatc gagtcgatcg
      901 cgccctcgtt ccttcgaagc cctggggccg cggcaagcgg caagacacaa ggtcttgccc
      961 tacagacccg ggctgatcgg gacgccttgt ggggaggatg ttcccgcctc caccgtcccg
     1021 ttcccgtccc ggcgacccac gtggtcgccc cggatcggct ccgccgatcc gtctttgggg
     1081 ggcatgatga gtgccgcggc aggacacacg gtcgtgcccc acaaacccgg gcagtccctc
     1141 ttcctcacgc gtgtggacga tcccgacctg acacgaatcc gctccgatcc ggcgtccaca
     1201 cgttgcccgc cgcacgccga acgcgtcggc ctgcgcatgc cgacttccct ccccccgaac
     1261 gggctggtat tgtggccgga ccacatcagc agccctgtcc cgaccgagga ggaaacgacc
     1321 gtgaccgacg ccgtcccgac aactccagac atgaccgtcg cggatcatca ccgaccgcaa
     1381 taccacgtca ccgcgccgca gaactggttg aacgatccca acgggctgat ccaatggcgc
     1441 ggcatctacc acatgttcta ccagtacacg ccggacaatc cccttggtgg cgtcaaggtc
     1501 tggggccacg ccatcagcca cgatctcgtc aactgggagc atctcccggt c
//
LOCUS       DASTMD010000218         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2162306, whole genome shotgun sequence.
ACCESSION   DASTMD010000218 DASTMD010000000
VERSION     DASTMD010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162306"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFQ54_03265"
     CDS             <1..282
                     /locus_tag="VFQ54_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0164036.1"
                     /translation="DGYIAANITPEDSPLVQAFQESVRAVTGKEPEYFVQWAGMTDGR
                     FYRQHDIDTVGMGPRGEGAHGANESISIDDLVLEGRIYAQTIATLLNAG"
     gene            complement(669..1610)
                     /locus_tag="VFQ54_03270"
     CDS             complement(669..1610)
                     /locus_tag="VFQ54_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0164037.1"
                     /translation="MTALKAGLAIRRGSAPDFVSRIVTAENQGVGQIWTTVGGISPDP
                     LTALAAAVVKTSSVVVGTSITPTYPRHHLALAAQAIAIDELAPGRLRLGIGPSHRPTI
                     EGLYNIPMGKPLSHLREYLTILRGLLWDGKVDFKGEYYQIPASLPDGAPAPKIPIPIS
                     ALRQNAFTLAGELADGAISWVTPVHFLVNTALPAMRAGAESAGRAKPPLIGHVPVAVS
                     TDRDAARNAFRAQFPAYSRLPFYVAMFEDAGFPVTPQGTMTDELVDNLAVSGTPDQVR
                     ARLEAILAEGVDELLISHVIVNDEAAELAELSAILAG"
     gene            complement(1736..>3104)
                     /locus_tag="VFQ54_03275"
     CDS             complement(1736..>3104)
                     /locus_tag="VFQ54_03275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0164038.1"
                     /translation="TPDGDRLVVNTTKVPVINEIIRKTSLWHVAISGGVLHHIADYPG
                     GAGSTVVRVIDGNLVAVSIASDGRDDPAASIWVVPLAGGTPRNLTPGYTGTFFHLEPD
                     PTDRAQVIAKIVEGVHSRIFRVNLATGDIAPIAPAGLAENGTISSDPSIAADGSAVAM
                     IWESSDKPREVFVGELGSEVTAITDFGSSFDERLFAGEKVTWKSTDGVKIEGILIRPP
                     GFVEGTRVPLIVEIHGGPSWQWEDRMMLDWHDWAQMLASRGYAVLLPNPRGSTGYGSD
                     FEKLLISDVGGGETQDIISGALAMVERGIADPEKLGVGGWSWGGFLTASTITQTTIFH
                     AAVMGAGVANLISDHAAGDIPEANMAYYPGHPYTHWDLYAKASPIRLAANAKTPTLIL
                     HGDSDIRVHPTQGQEFYRALKENGVPVQFVKYPREGHGFQERAHQIDLMKRIVDWYER
                     YIPAI"
BASE COUNT          530 a          963 c         1067 g          544 t
ORIGIN      
        1 gacggctaca ttgcggcgaa catcacgccg gaggattcgc cgctggtgca ggcgttccag
       61 gagagcgttc gtgccgtgac cggcaaggag ccggagtact tcgtgcagtg ggcgggaatg
      121 accgatggtc gcttctatcg ccagcacgat atcgacacgg tggggatggg gccgcgcggc
      181 gagggtgccc acggcgcaaa cgagtcgatc tcgatcgacg acctcgttct cgagggacgc
      241 atctacgcgc agacgatcgc gacgttactg aacgccgggt agaacgtcag gcgggacggg
      301 ccgtccggac ccgtagcggg tccgctggtc tggcccggca gcgcaccgat cgaccgcagg
      361 tcgatctgat cccggcatgg accacgcccg cgggcccgtc ttcgccccgg gtcccgccct
      421 acgaacgacc gctgcatcaa cggcgtcgca ttgtgttcgc catgtacgga acaccgttcc
      481 cgcagcgagg ggaataggga acgcgtcgca ccgggagcgc tttctggcgg gacatagaag
      541 tcccgccgta ctcacggacg ctgtatcaat ggcgtcgcac cgtattcgtc atgcggggaa
      601 caccgttgga tcggccgggg tttggaaggc gagatttcat atcccgccgc gacggcgact
      661 ccggctggtt atccggcgag gatcgccgag agttcggcga gctcggccgc ctcgtcgttc
      721 acgatgacgt gggagatcag caattcgtca acgccctcgg cgaggatcgc ttcgagccgg
      781 gcgcggacct ggtcgggtgt gccggagacg gcgaggttgt cgaccagctc gtccgtcatc
      841 gttccctgcg gcgtgaccgg gaaaccggcg tcctcgaaca tcgccacgta gaaggggaga
      901 cggctgtagg ccgggaactg ggcacggaac gcgttccgcg cggcgtcgcg gtccgtcgag
      961 acggcgaccg ggacatgccc gatcaggggt ggtttcgcgc gaccggccga ttcggcgccg
     1021 gcccgcattg ccggcagggc cgtgttgacg aggaagtgaa ccggggtcac ccaactgatc
     1081 gcgccatcgg cgagttcacc ggcgagcgtg aaggcgttct gccgcagcgc ggagatcgga
     1141 atcgggatct tcggagcagg tgcgccgtcc ggcaggctgg cggggatctg gtagtactcg
     1201 cccttgaaat cgaccttgcc atcccagagg aggccgcgca ggatcgtcag gtactctcgc
     1261 agatgggaaa gcggcttgcc catcgggatg ttgtagagcc cctcgatcgt ggggcggtgg
     1321 ctcggtccga tcccgaggcg gagccgtccc ggagcgagtt cgtcgatcgc gattgcctgc
     1381 gccgccagcg ccaggtgatg acgcgggtac gtcggtgtga tcgacgtccc gacgacgacc
     1441 gatgacgtct tcaccacggc ggcggcgagc gccgtcaacg ggtccggcga gatcccgcca
     1501 accgtcgtcc agatctggcc gacaccctgg ttctcggccg tgacgatacg ggagacgaaa
     1561 tcgggcgccg agccgcgtcg gatcgcgagt cctgccttga gtgctgtcat aggtgatgtc
     1621 cttctcgtgc gtttccgatg cgaacgatga acggtggacc cctggaaaag aggtccgccg
     1681 ttcatcttac ttcaatgcta tgttttggga aggaactgat gttcgagata gcgatctaga
     1741 tcgcggggat atatcgctcg taccagtcga cgatccgctt catgaggtcg atctggtggg
     1801 cgcgctcctg gaatccgtgg ccttcgcgcg ggtacttcac gaattgcacc ggcacgccgt
     1861 tttccttgag ggcgcggtag aactcctggc cttgcgtcgg atgaacgcgg atatcgctgt
     1921 cgccatggag gatcagcgtc ggggtcttcg cattcgcagc gagacggatc ggcgaggcct
     1981 tcgcgtagag gtcccagtgc gtgtagggat ggccgggata gtaggccatg ttcgcctcgg
     2041 gaatgtcccc cgccgcatga tcggagatca ggttggcgac gccggcgccc atcacggccg
     2101 cgtggaagat cgtcgtctgg gtgatcgtcg aggcggtcag gaagccgccc cagctccagc
     2161 cgccgactcc gagcttctcc ggatcggcga tgccccgctc gaccatcgcc agggcgccgc
     2221 tgatgatgtc ctgcgtctcg ccacccccga cgtcggagat gagcagcttc tcgaaatcgc
     2281 tgccgtatcc ggtgctccca cggggattcg ggaggagcac ggcgtagccg cgtgaggcaa
     2341 gcatctgtgc ccagtcgtgc caatcgagca tcatccgatc ttcccactgc cacgatggac
     2401 caccgtggat ctcgacgatc agcggcacac gggtgccctc gacgaaaccg ggcggcctga
     2461 tcaggatgcc ctcgatcttg acaccgtcgg tgctcttcca ggtgaccttc tctccggcga
     2521 agaggcgctc gtcgaacgac gagccgaagt cggtgatcgc cgtcacctcg ctaccgagtt
     2581 cgccgacgaa gacctcgcgc ggcttgtcgg aggactccca gatcatggcg accgcactac
     2641 cgtccgccgc gatcgacgga tcggaggaga tcgtgccgtt ttcggcgaga cccgccggtg
     2701 cgatcggcgc gatatcgccc gtcgcgaggt tcacgcggaa gatgcgtgaa tgcacaccct
     2761 cgacgatctt cgcgatcacc tgcgcgcggt cggtcggatc gggctcgaga tggaagaagg
     2821 tacccgtgta gcccggcgtc aggttccgtg gcgtcccgcc ggccaggggg acgacccaga
     2881 tcgaggcagc tggatcgtca cgaccgtcgc tggcaatcga cacggccacg aggttgccgt
     2941 caatgacgcg gaccacggtc gagccggcgc cgcccggata gtcggcgatg tgatggagaa
     3001 cgccgccgga aatcgcgacg tgccagagtg acgtcttccg gatgatctcg ttgatgacag
     3061 gaaccttcgt cgtgttgacg acgaggcggt cgccatccgg cgtc
//
LOCUS       DASTMD010000219         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1358411, whole genome shotgun sequence.
ACCESSION   DASTMD010000219 DASTMD010000000
VERSION     DASTMD010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358411"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..911)
                     /locus_tag="VFQ54_03280"
     CDS             complement(159..911)
                     /locus_tag="VFQ54_03280"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0164039.1"
                     /translation="MNKLENKVAVVTGASKGIGAAIAKQLASAGASVVVNYATSRQGA
                     DMVVREIIDGGGTAIAMPADVTNESEVDALFLDVKDRYGRVDIVVNNAGIYSFAPLGG
                     ITTELYRKIFDINVLGVLLVSRAALPLFPESGGTIINISSVVSALTPPTASIYASSKA
                     AVDAITKSLAKELGPRKIRVNALNPGVIRTEGLAAAGIADSELEAQLVSMSAIARVGY
                     PHDVALPAVFLASDDARYITGATLFVSGGAGM"
     gene            complement(908..1978)
                     /locus_tag="VFQ54_03285"
     CDS             complement(908..1978)
                     /locus_tag="VFQ54_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0164040.1"
                     /translation="MNHATHHTAPTQFVEANGIRFAYRRFGRKEGIPLVFNQHLMGNL
                     DSWDPAVTDGLARTREVILFDNAGVASSSGEVPQTFALIAKDAEAFIDALGLTRVDVL
                     GFSIGSMVSQLIALDRPDLVRRLILVGSSPRNGDSLPLTPESEVVFGTEYADPDDFWI
                     NGFFTHSPASLAAGQAFLKRRDLRTVDRDVPPDDRVQAAQFAAFTEWANPVGDRFAYL
                     QDIKMPVLIVNGKSDIVVYAINSLHLEQNLPDAQLIIYPDSAHGSLFQYPELFVEHAT
                     MFLRDAREKPTDERQATVNEKRREAIVLSEPMHAGRDHQEDTIDEALLETFPASDPIA
                     VTSAGMHSRQRPDSDHVSSARQ"
BASE COUNT          431 a          726 c          835 g          463 t
ORIGIN      
        1 gctgcgggtg atgaggcggt acttcttcga ggcggtcgtc atgcggaagg ctcctgaggc
       61 gggggtgggc tcaggagtta tcggcggcga tggggagagc ttgaggcgtg ggctgccacc
      121 agctcggatt tcgaccgcga aagcgggcgg gccgctcgtc acatgccggc gccgccagag
      181 acgaacagcg tggcgcccgt gatgtagcga gcatcgtccg acgcgaggaa aacggcggga
      241 agtgcgacgt cgtgcgggta tcccacacgc gcgatcgccg acatcgaaac cagctgcgcc
      301 tcgagctcgg agtccgcgat gccggcggct gcgagtcctt ccgttctgat cacgccaggg
      361 ttgagggcgt tgactcgaat tttcctcggg ccgagttcct tcgccaggga cttggtgatc
      421 gcatccacgg cagccttcga actcgcgtag atcgacgctg tcggcggggt gagcgcgctc
      481 accacagagc tgatgttgat gatcgttcca cccgactccg gaaagagcgg aagcgccgct
      541 ctggatacga ggaggacgcc gaggacgttg atgtcgaaga tctttcggta gagctcggtg
      601 gtgatccctc ccagcggcgc gaagctgtag atccccgcgt tgttcacgac aatatccacg
      661 cgaccgtagc ggtccttcac gtcgaggaag agggcgtcga cctcgctctc gttcgtgacg
      721 tcggccggca tcgcgattgc cgtcccgccc ccgtcgatga tctcgcgaac gaccatgtcc
      781 gcaccctgac gactggtcgc gtagttgacc accaccgagg cgcctgcgct ggcgagctgt
      841 tttgcgatgg cggcgccgat ccccttggat gcgcccgtca ccactgcgac cttgttctcg
      901 agcttgttca ttggcgtgcg ctcgacacgt gatccgaatc aggccgctgg cgcgaatgca
      961 tgccggcgga agtgacggcg atcgggtcgc tggccgggaa tgtctcgagc agcgcttcgt
     1021 cgatcgtgtc ttcctgatgg tcgcgccccg cgtgcatcgg ctcggacagt acgatcgcct
     1081 cgcgacgctt ctcgttcacc gtagcctgcc tttcgtccgt cggcttctcc ctcgcgtcgc
     1141 gaaggaacat ggtcgcgtgt tcgacgaaca gctccgggta ctggaagagc gagccgtgtg
     1201 cggaatccgg gtagatgatc agctgcgcat cgggaaggtt ctgctcgaga tgaagcgaat
     1261 tgatcgcgta gacgacgatg tcggacttcc cgttgacgat cagcaccggc atcttgatgt
     1321 cttgcaggta ggcaaaacga tcgccgacgg ggttcgccca ctccgtgaac gcggcgaact
     1381 gcgcggcttg cacccgatcg tcgggtggga cgtcccggtc gacggtccgc agatcgcgcc
     1441 gcttcagaaa ggcctgcccg gcggcgagac tcgccggcga atgcgtgaag aagccgttga
     1501 tccagaagtc gtcgggatcg gcgtactcgg taccgaagac cacctcggat tcgggcgtga
     1561 gcgggaggct gtcgccgttg cgcggggaac tgcccacgag gatgagcctg cggacaagat
     1621 cgggacgatc caacgcgatg agctgcgaca ccatcgaacc gatcgagaaa cccaggacgt
     1681 cgacccgcgt caagccgagc gcatcaatga atgcctcggc gtccttggcg atgagcgcga
     1741 aggtctgcgg aacctcgccg ctcgagctcg cgactccggc gttgtcgaac aggatcactt
     1801 cacgggtccg cgcaaggcca tctgtgacgg caggatccca gctgtcgagg ttgcccatca
     1861 ggtgctggtt gaagaccagg ggaatgcctt cttttctccc gaagcggcgg tatgcgaagc
     1921 gaatgccgtt cgcttcgacg aactgagtcg gtgcggtgtg gtgcgtggcg tggttcattg
     1981 aagtctctgg tctggagtga ggggggcggg cgtgccgcca tgtcggtacg gtcgtggggg
     2041 gcaatgcgac gggatcgcta cggcacgggc agcgacctcg cgcgccggtc cggggcgtct
     2101 cggtcgtttc tgcgcgccgg gatggttgga tcacctggcg agccggacgg tctcactcaa
     2161 gagggattct caagcgtaaa aacgatacga gccgtttcgt atccgtccat tgccaagaat
     2221 acgcttcgta tcgtttcgta tgtagaatcg catcgattct ttttcaggca ccacgaacga
     2281 tcatggagcg atcgctggaa gcagtcagag gaagggggcg cccgcgcagc gaccgtctgc
     2341 accaagcgat tctcaaagcg gcgctcgacc tggtgctgga cgtcggtttc cgggcggtca
     2401 gtgtcgaagc aatcgcggcc aggaccggcg tcggcaagac gaccatctat cggcg
//
LOCUS       DASTMD010000220         1990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_824255, whole genome shotgun sequence.
ACCESSION   DASTMD010000220 DASTMD010000000
VERSION     DASTMD010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824255"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(605..>1990)
                     /locus_tag="VFQ54_03290"
     CDS             complement(605..>1990)
                     /locus_tag="VFQ54_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164041.1"
                     /translation="SSDPVGFGTKIIPSLAKSWKRLDDLTMEVVLRDDILFHDGTPVT
                     ADDVKYTIDRIIDPKADPKLQVVIVAFQTYDHVDVIDTHTLHIVTKAPDIALEQRFAL
                     LYIVSRAAAEAQGLAGYALKPIGAGPYKVTEFVANDHLTFESFDQYYGGLPPFSKITV
                     RAIPEVATRIAGILSNEVQLISDVTPDQIPSLQSDDSIQVNSIASANAHFFFYNTKMP
                     VLADKRIRQGLNMAIDRQLIIDTLWMGNADMMSSYQVPDWGDMYNPDRPTPKYDPDQA
                     KQLIQAAGYDGTEIKFRVPTTYYTLGADAAQAIVSMWQDVGVKASVELNDDPHGDKPH
                     LMVCFWSAALTPADPTVTFSNIWAEKGEPQTYFWTPADPKFNQNLDFLTTSLDAKARY
                     TAYQQILDIWDDEAPASMLYRVHSIYGQKKTVGWTPNTTFEMDLRASNIGKGDASDAT
                     PGASPEASPAS"
BASE COUNT          332 a          578 c          683 g          397 t
ORIGIN      
        1 cgacgggcac gccatccttc gagacgtgga tgtcgagctc gatcaggtcg gcctcgatcc
       61 ggatggcgtt ggcgaatgcg gcgagggtat tttcgggggt cccggtggac ccgccccgat
      121 gggcaacgac gcgcatgaga tgacccttcc cgtaactaca gcgaaaactg aaacggttgc
      181 cccttggggg cggggcgtac ttcgcccgag tccgctttgc ggcggggtat gaaaccccgc
      241 cctaccaacc ccagcaccag caaaacgggt cgacggttct ggatttcgat gtaaagtcgt
      301 gcgatcgaat tggtcctggc gcgtcagccc gggatcgggc tgcggcgaga gatgaactct
      361 cgccctgcaa ccccgggtcc ccccttcgga ggagatgcga cgccgcgtca ggggtgccgt
      421 ccgccgccgg ttcgtgttgg cggcgagaga tggactctcg ccctacaaac cccggtccca
      481 ccgaaaatca gtcgcgtgag gcatctttgt ctcagcggtc gttcgtacgg cggacccatt
      541 cggctccgct cagggcaggc ttttcatgtc ccgcctccgg aggagtcgaa gagccctccg
      601 gtacctacga cgcggggctt gcctccgggg aagcccccgg tgtcgcgtcc gacgcgtcgc
      661 ccttgccgat gttgctggcg cggagatcca tctcgaaggt ggtgttcggc gtccagccga
      721 cggtcttctt ctggccgtag atggagtgga cgcggtagag catggaggcc ggggcctcgt
      781 catcccagat gtcgaggatc tgctggtagg cggtgtagcg cgccttcgca tcgaggctcg
      841 tggtcaggaa atcgagattc tggttgaact tcggatcggc cggcgtccag aagtaggtct
      901 gcggttcccc cttctccgcc cagatgttgg agaaggtcac ggtcggatcg gccggggtga
      961 gcgccgccga ccagaagcag accatcaggt gcggcttgtc gccgtgggga tcgtcgttga
     1021 gctcgacgct tgccttgact ccgacgtcct gccacatcga gacgatcgcc tgcgcggcgt
     1081 cggcaccgag cgtgtagtag gtggtgggga cgcggaactt gatctcggtg ccgtcgtagc
     1141 cggcggcctg gatcagctgc tttgcctgat ccggatcgta tttcggcgtc ggacggtccg
     1201 ggttgtacat gtctccccag tccggcacct ggtagctgct catcatgtcg gcgttaccca
     1261 tccagagggt gtcgatgatg agctggcgat cgatcgccat gttcaggccc tggcggatcc
     1321 gcttgtcggc gaggaccggc atcttcgtgt tgtagaagaa gaaatgagcg ttcgccgatg
     1381 cgatcgagtt gacctggatc gaatcatccg actgcagcga cgggatctgg tccggggtga
     1441 catcggagat gagctggacc tcgttgctga ggatgccggc aatgcgggtc gcgacttccg
     1501 gaatcgcgcg aacggtgatc ttcgagaagg gcggcaggcc accgtagtac tgatcgaagg
     1561 attcgaaggt gaggtgatcg ttggcgacga actcggtgac cttgtacggt cccgcgccga
     1621 tcggcttcag ggcgtagccg gcgagtccct gtgcctcggc cgccgcccgg gagacgatgt
     1681 agaggagggc gaagcgctgc tcgagggcga tatccggggc cttggtcacg atatgcaggg
     1741 tgtgggtatc gatcacgtcg acgtggtcgt aggtctggaa ggcgacgatc acgacctgca
     1801 gcttgggatc ggccttcgga tcgatgatcc gatcgatcgt gtacttcaca tcgtccgccg
     1861 tcaccggcgt gccatcgtgg aagaggatgt cgtcacgaag cacgacttcc atcgtgagat
     1921 catcgaggcg cttccatgac ttcgcgagcg acgggatgat cttcgtcccg aagccgaccg
     1981 ggtcgctgga
//
LOCUS       DASTMD010000221         3487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1807278, whole genome shotgun sequence.
ACCESSION   DASTMD010000221 DASTMD010000000
VERSION     DASTMD010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807278"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFQ54_03295"
     CDS             complement(<1..187)
                     /locus_tag="VFQ54_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164042.1"
                     /translation="MSDEKGLEQQPGSAPLFSAGMCRVFHHTISSHKSQQPYNMPVRR
                     CHPMNAIIVQNRFRGEVC"
     gene            217..1947
                     /locus_tag="VFQ54_03300"
     CDS             217..1947
                     /locus_tag="VFQ54_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0164043.1"
                     /translation="MLRRFFSYYARYKTLFFVDFGCAVLAGLLELCFPLAVKLFIDKL
                     LPSHDWGLIGITAVVLLVIYALNTGLMAVVNYWGHALGISIETDMRRQAFDHIQRLSF
                     RYFDNNRTGHLITHVTKDLEEVGEIAHHGPEDVFIAIMTFIGAFILMFFIEPKLALIT
                     AVIVPVIGFVTSRYGGRMTKTMREMFGRVGEFNARIENNVGGIRVVQAFANEDHEREL
                     FAVDNKAYRKTKLEAYRTMTASLTLSYLTMRLTQLIVMVAGTYFVINGELSNGGFVSF
                     LLLIAVFFRPVDKINAVLESYPKGIAGFKRYQELLDIDPDIADTPGSRDVSNLRGEIR
                     YEHVDFGYTSDRLILNDLSLVIHPGETIAFVGPSGAGKTTISSLLPRFYEVLAGRITI
                     DGIDIRDMTLASLRREIGIVQQDVFLFGGSIRENIEYGKLGAPVEEILEAARRARLDE
                     VIAGLPAGIDTVIGERGVKLSGGQKQRLAIARMFLKNPPILILDEATSALDTETERAI
                     QQSLQELAVGRTTLVIAHRLATIQNADRIVVVDKTGVAEQGRHEDLVQLNGIYRRLHD
                     AQYGTAAVAD"
     gene            2206..>3487
                     /locus_tag="VFQ54_03305"
     CDS             2206..>3487
                     /locus_tag="VFQ54_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0164044.1"
                     /translation="MEENRQAMREALKLVESQLGQRYPIVIGGERRETGQWITSINPG
                     NLDQVVGEVAKGSAEDVELAIAAAQRAFESWRRIPVEGRASVLFKMAAILRRRRYELS
                     AWMTYELDKTWDEADGEVAEAVDFLEWYGRQALKLAERVPLSHLEGEATEYRYQPVGV
                     GIAIPPWNFPCAIFTGMTMAPVAVGNAVIVKPATNTPVIGYKMFEIMEEAGVPAGVVN
                     FLPGSGAEIGDKLVEHPKTRFVSFTGSRDVGVRIYERAAKVQPGQRWLKRVVAEMGGK
                     DAIIVDASADIEAAVEGIVTSAFGFSGQKCSACSRVIVDASVAEAVTNGVVERTKRLV
                     SVGSAIAGETSTGAVVDKNQYESIMKYIELGGSEGREVYRGEVPANANGYYVPPVIFA
                     DVPPTARIACEEIFGPVLAIVPVASFDEALDVAND"
BASE COUNT          637 a         1132 c         1102 g          616 t
ORIGIN      
        1 ggcagacctc gccgcgaaat cgattttgca cgatgatcgc attcatgggg tgacagcggc
       61 ggaccggcat attataaggc tgctgcgact tatgagagga gatcgtatga tggaaaactc
      121 ggcacattcc agcgctgaat agcggtgccg atccgggctg ctgttccagt cctttctcat
      181 cagacatcag gtcttctgcg cccctggagg gcactcatgc tgcgtcgctt cttctcctac
      241 tatgcgcgtt acaaaacgct gtttttcgtc gatttcggct gcgccgtcct ggccgggctg
      301 ctcgaactct gctttccgct ggcggtcaag ctcttcatcg acaagctgct gccgagccat
      361 gactggggcc tgatcggcat caccgccgtc gtgctgctcg tcatctatgc gctgaatacc
      421 gggctgatgg ccgtggtcaa ttactggggc catgcgctcg gtatctccat cgagacggac
      481 atgcgccggc aggccttcga ccatatccag agattatcct tccgttattt cgacaacaat
      541 cgcaccgggc atctcatcac ccacgtcacc aaggatctgg aggaggtcgg cgagatcgcc
      601 caccacggtc cggaggacgt gttcatcgcg atcatgacct tcatcggcgc cttcatcctg
      661 atgttcttca tcgaaccgaa gctcgccctg atcaccgccg tcatcgtccc ggtgatcggc
      721 ttcgtcacgt ctcgctacgg cggccggatg acgaagacga tgcgcgagat gttcggtcgc
      781 gtcggcgagt tcaacgcccg gatcgagaac aacgtcggcg gcattcgcgt cgtccaggcg
      841 ttcgcgaacg aggatcacga gcgggagctc ttcgcggtcg acaacaaggc ctaccgcaag
      901 accaagctcg aagcctaccg gacgatgacg gcaagcctga cgctcagcta cctgacgatg
      961 cggctgacgc agctgatcgt gatggtcgcg ggcacctact tcgtgatcaa cggcgagctt
     1021 tcgaacggtg ggttcgtcag cttcctcctg ctgattgccg tcttcttccg accggtcgac
     1081 aagatcaacg cggtgctgga gtcgtatccg aagggaatcg ccggcttcaa gcgctaccag
     1141 gaactgctcg atatcgaccc cgacatcgcc gatacgccgg gctcgcgcga cgtgagcaac
     1201 ctccgcggcg agatccgcta cgagcacgtc gatttcggct atacctcgga tcgcctgatc
     1261 ctgaacgatc tcagccttgt gattcacccg ggtgagacga tcgccttcgt cggtccgtcg
     1321 ggtgccggca agacgacgat ctcgtcgctg ctcccgcgct tctacgaggt cctcgccggt
     1381 cgcatcacga tcgacggcat cgacatccgg gacatgaccc tcgcctcgct ccggcgcgag
     1441 atcggaatcg tgcagcagga cgtcttcctc ttcggcggct cgattcgcga gaacatcgag
     1501 tatggcaagc tcggggcgcc ggtcgaggaa atcctggagg cggcgcggcg ggccaggctc
     1561 gacgaggtga tcgccggcct cccggccgga atcgacacgg tcatcggcga gcgcggcgtc
     1621 aagctctcgg gcggccagaa gcagcggctc gcgatcgccc gcatgttcct gaagaacccg
     1681 ccgatcctca tcctcgacga ggcgacctcc gccctcgaca cggagacgga gcgcgcgatc
     1741 cagcagtcgc tccaggaact cgcggtcgga cgcacgacac tcgtgatcgc acaccggctg
     1801 gcgacgatcc agaacgccga ccggatcgtg gtcgtcgaca agacgggcgt cgccgagcag
     1861 gggcgccacg aggatctggt gcagttgaac ggaatctacc gccgcctcca cgacgcgcag
     1921 tacggcaccg ccgccgtcgc cgactagcat cccggaccaa gccgtcgcga gagtggcccg
     1981 gcacgcaggt gggatggacg acccccccgc gcgcgaggca gccgcgatgc atcgcgcggg
     2041 atacggggat cgtgcgaaac tgagcgtgct tgaatccgcc ggtcgggatg accggcgttc
     2101 tttggataca gctggcgatg gcacatcctc gccgcaatga cgcccagaag aggaccaaca
     2161 gcaatgcgtg gaccgttcag taatgagccc ggaatcgatt tctcggtgga agagaatcgc
     2221 caggcgatgc gcgaggcact gaagctggtc gaatcccagc tcggccagcg ctacccgatc
     2281 gtgatcggtg gcgaacgccg cgagaccggc cagtggatca cctcgatcaa cccgggcaat
     2341 ctcgatcagg tcgtgggcga agtcgcgaag ggctcggccg aagacgtcga actcgccatc
     2401 gccgctgccc agcgcgcatt cgagagctgg cgcaggattc cggtcgaggg gcgcgcgagt
     2461 gtgctcttca agatggccgc gatcctccgc cgtcgccggt atgagctctc cgcctggatg
     2521 acctatgaac tcgacaagac atgggacgaa gcggacggcg aggtcgccga ggccgtcgac
     2581 ttcctcgagt ggtacgggcg gcaggcgctg aagctcgccg agcgcgttcc gctctcccat
     2641 ctcgagggtg aggcgaccga gtatcgctac cagccggtcg gcgtcggcat cgcgatcccg
     2701 ccgtggaact ttccctgcgc gatcttcacg ggcatgacga tggccccggt cgccgttggc
     2761 aacgccgtca tcgtgaagcc ggcaaccaac accccggtca tcggctacaa gatgttcgag
     2821 atcatggagg aggccggcgt tccggccggc gtcgtcaact tcctgccggg cagcggcgcc
     2881 gaaatcggcg acaagctggt cgagcatccg aagacccgct tcgtctcctt caccggctcc
     2941 cgcgatgtcg gggtccgcat ttacgagcgc gccgcgaagg tccagccggg tcagcgctgg
     3001 ctgaagcgcg tcgtcgccga aatggggggc aaggacgcga tcatcgtcga cgcgagcgcg
     3061 gatatcgagg ccgcggtcga aggcatcgtc acctccgcct tcggattttc cggccagaag
     3121 tgctccgcct gctcccgcgt catcgtcgac gcctcggttg ccgaggccgt gacgaacggc
     3181 gtggtcgagc gcacgaagcg cctggtcagc gtcgggtccg cgatcgccgg tgagacgagc
     3241 accggcgcgg tggtcgacaa gaaccagtac gagtcgatca tgaagtacat cgaactcggc
     3301 ggatcggaag gacgcgaagt ctaccgcggc gaagttcccg ccaacgcgaa cggctactat
     3361 gtgccgccgg taatcttcgc cgacgtgccg ccaaccgcgc gcatcgcctg cgaggagatc
     3421 ttcggtccgg tcctcgcgat cgtcccggtt gcctcgttcg acgaggcgct cgacgtcgcc
     3481 aacgact
//
LOCUS       DASTMD010000222         3000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_824703, whole genome shotgun sequence.
ACCESSION   DASTMD010000222 DASTMD010000000
VERSION     DASTMD010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824703"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFQ54_03310"
     CDS             <1..675
                     /locus_tag="VFQ54_03310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEU0164045.1"
                     /translation="AHRSVDSMRDTPTLRASIMTIPSATFVSTGKPRILGIGGSMNAV
                     SRNRVVTRHLLDLAEDAGAETVFADVRELALPIYIEDTPYDEQPAALHWLIAQIQAAD
                     GFIIDSPNYHGTISGAVKNALDAVHILHGADRSYFDNRPVALACYGGPSSQNVINALY
                     HCVRGMRGLTVPTVVHVGSNQIEMAGDTGRISDERTLVRAQAMIGEVLDLIDLRRARD
                     LAAAAD"
     gene            692..1144
                     /locus_tag="VFQ54_03315"
     CDS             692..1144
                     /locus_tag="VFQ54_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0164046.1"
                     /translation="MSSRITPTGIHELVLEVGDLAASTHFYTEIVGLEVITRWGDDRP
                     AVWLDMGDTTALGLWPVETGGAKAIAGGRGGVHVHFAFRIPAGSTDAMQAQLEAKGVR
                     IVERVDFDDGNRSLYLDDPDGNCVELMDAIVDWSLNPIPSTASKGSPT"
     gene            1141..1506
                     /locus_tag="VFQ54_03320"
     CDS             1141..1506
                     /locus_tag="VFQ54_03320"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEU0164047.1"
                     /translation="MTATTIDSSLATERNGYLVTTGRTSGLPRETEIWFITGENAIHV
                     FSGEHNNKDWFRNLQKTPQVRFRIRDTWFDGTARIDSTAGADRTRLEQEFNDKYYGGQ
                     PSGEHDWPSRATLVTIDLA"
     regulatory      2173..2285
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2625..>3000
                     /locus_tag="VFQ54_03325"
     CDS             2625..>3000
                     /locus_tag="VFQ54_03325"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164048.1"
                     /translation="MGTYILRRAALAIPTLIFLTLAAFLLTSASRGDPAEAALRAGGI
                     EPTREAIAEYREKLGLNDPLIVRYGRWVFNLSHGDLGRSFLDGRPVSEIIGERLGPTF
                     RLGIAAFVTSTIMGIGLGIVFGL"
BASE COUNT          544 a         1040 c          896 g          520 t
ORIGIN      
        1 gcgcatcgaa gcgttgattc gatgcgcgac acgccgacct tgagggccag catcatgacg
       61 attccatccg cgaccttcgt ttcgaccggg aagccccgca ttctgggcat cggcggcagc
      121 atgaatgccg tctcccgcaa tcgcgtcgtc acgcgacacc tgctcgatct cgcggaggac
      181 gcgggtgcgg agaccgtctt cgccgacgtt cgcgagctcg cgctgccgat ctacatcgag
      241 gacaccccgt acgacgaaca gccggcggcg ctccactggc tgatcgcgca gatccaggcg
      301 gcggatggct tcatcatcga ctccccgaac tatcacggga cgatttcggg cgccgtgaag
      361 aatgcgctcg acgcggtgca catcctgcat ggcgccgacc gcagctactt cgacaatcgc
      421 ccggtcgcgc tggcctgcta cggcggtccg tcgtcgcaga acgtgatcaa cgcgctctat
      481 cactgcgtcc gcggcatgcg cggtctcacc gtgccaacgg tcgtccatgt cggcagcaac
      541 cagatcgaga tggcgggcga caccggtcgc atctcggacg aacgcacgct ggtccgggcc
      601 caggcgatga tcggcgaagt cctcgatctg atcgatctcc gccgggcgcg cgatctcgcc
      661 gccgcggcgg actaaggcaa gggagccatc gatgtcttcc cggattaccc cgaccggcat
      721 tcacgaactc gtgctcgagg tcggcgacct cgcggcatcg acgcacttct acacggagat
      781 cgtcgggctg gaggtgatca cccgctgggg cgacgatcgc cccgcggtct ggctcgacat
      841 gggggacacg acggcgctcg ggctctggcc ggtcgagacc ggcggtgcga aggcaatcgc
      901 cggcgggcgg ggcggcgttc acgtccactt cgcgtttcgc atccctgccg gatcgaccga
      961 cgcgatgcag gcgcaactcg aggcgaaggg cgttcgcatc gtcgagcgcg tcgacttcga
     1021 tgacggcaac cgctcgctct acctcgacga ccccgacggc aactgcgtcg aactcatgga
     1081 tgccatcgtc gactggtctc tcaatcccat tccgtctacc gcatcgaaag gatccccaac
     1141 ttgaccgcaa ccacgatcga ttccagtctc gcgacggaac gaaacggcta tctcgtcacg
     1201 accggtcgca cgagcggtct tccccgcgag accgagatct ggttcatcac cggagagaat
     1261 gcgatccacg tcttctcggg cgagcacaac aacaaggact ggttccgcaa tctccagaag
     1321 acgccgcagg tccgcttccg cattcgcgac acctggttcg acggcaccgc gcgcatcgac
     1381 tcgacagccg gcgccgaccg gacgcgcctc gagcaggaat tcaacgacaa gtactacggc
     1441 ggccaacctt ccggcgagca tgattggccg tcccgcgcga ccctcgtcac gatcgacctc
     1501 gcgtagagcg aaattcggaa tcgtcgcgac ggggagcacg atccgtgggc gaccggcagg
     1561 cgggacaggc cgtccggacc tccggtccgg cagcggcggt cccgccctac gaacgaccgg
     1621 gcgcaccgcg gactacgcat tcggtccagc agcacgttcg gcaaccgttt cggatttcgc
     1681 tgcagaccgc gcagggcgac caccagggtc gcccctacgg aattaccaac catcgcaggg
     1741 gcgaccctgg tggtcgccgt acccttcgcg tccgccgtgc ttccctcacg actccgcaca
     1801 aaatacgtcc tcccgagctt gtcgagcgac cagttgacat ccgcgtgttc cccctacgac
     1861 gcgctcgtcg aggagggagg cagcgaggca acgcgaatgg aacgtggtcc cgggcgaaca
     1921 ccaggttcgc ccctacggac gcgcgcctga cggcgccgcg tctgcgctcc gcccggatga
     1981 cgtgtcatgg cggggccgca ggacatcgat ttccgacacc cgggacccac agcaatcctt
     2041 tatgcaacac gcatatcttt gccatcctct ccctccttcc accgaaaatt ccggacgggg
     2101 aacatgaaat acgggttgac actcgcccct ctctccggta acgtcatatc cacaagtgaa
     2161 gaaacgcacg atgtctcatc aagaggagca gagggaacgg cccgatgaag ctccagcaac
     2221 cccccgtcac gattgccggg aaggtgctaa tgccgatcag tcgcgcaagc gctgaccgat
     2281 gagggattcg ttcgccagac tggtcaacca ccatttcggc aacacgagtc aacgcctctt
     2341 ccgttcagca acggaagagg cgttttttgt gcgtggacga ttgtcggccg gtcgcggacc
     2401 agtcgaccat tcagggagtt ccgaggtgag cgaacttcat ccaaatccag gctgtatccg
     2461 ggccagggcg atctgccttg tcctggccag cttctcctgt tgatctgatc cctccgcgct
     2521 gacgacgagc gcgccacgac ccgggtcatg tccgcgacga atgtcgcggg ccagcattgc
     2581 tgtatgccat tttcctgcga ccacgcgaca tcaggagtcc tttcttgggt acctacattc
     2641 tccggcgagc cgccctcgcc attcccaccc tgatcttcct gaccctcgcc gcctttctcc
     2701 tgacgagtgc ctcgcgcggc gatccggccg aggcagcact ccgcgccggt ggcatcgaac
     2761 cgacccgtga ggcgatcgcc gagtaccggg agaagctggg cctgaacgat cccctgatcg
     2821 ttcgctacgg acgctgggtg ttcaatctca gccacggtga cctcggtcgc tccttcctcg
     2881 acggtcgccc ggtctccgag atcatcggcg agcggctcgg cccgaccttc cggctcggga
     2941 tcgccgcgtt cgtcacctcg accatcatgg gaatcgggct cgggatcgtc ttcgggctgc
//
LOCUS       DASTMD010000223         3448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_646550, whole genome shotgun sequence.
ACCESSION   DASTMD010000223 DASTMD010000000
VERSION     DASTMD010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646550"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VFQ54_03330"
     CDS             <1..457
                     /locus_tag="VFQ54_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164049.1"
                     /translation="VMVATSLILCELLASSRNTIITFFVCHWAAAIGTILMLRLLSGL
                     GMDRATVLLTIPDAGSSASVHGAYAVAAMMLPPRLATIAYGILLGITVVLLFQQSLDA
                     AIAHLLAVGIGGAIGLLIFRPRMIATFTSSHAATDLATDPLQTETARGV"
     gene            complement(629..1552)
                     /locus_tag="VFQ54_03335"
     CDS             complement(629..1552)
                     /locus_tag="VFQ54_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164050.1"
                     /translation="MSDPGAASLSQSGYAQPRRGFRIRLSPETRRAFRKFRQSPLPLA
                     GLTIFVAIVLIAIFGPFFVPYPKDASNAVHFGDKLKAPSWDHWFGTDQAGRDILTRVV
                     VGARASLLAGFIVIILAVSIGTLLGALAGFFGGWIGNIIMRITDIFLTIPDLILAMAF
                     AAALGQWLFKVMIAISLVWWPGYCRLVRANVISLRQSQFVEAADALGASRMRILFRHI
                     LPNAFPTVLVKASMDVGFAVLTTAALGFIGLGSQPPAPDWGQMVSDGRNYIRDAWWFS
                     TFPGLAILLTVVASNLMGDGLRDVFDPRSRK"
     gene            complement(1596..2300)
                     /locus_tag="VFQ54_03340"
     CDS             complement(1596..2300)
                     /locus_tag="VFQ54_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164051.1"
                     /translation="MLVAMVIAVAVGVPLGVLAAVRADRLADHLSLIISLMFLSVPAF
                     WLAILMQLFLGLKLGWLPISGRLEALTIPPPHVTGLYLVDSLLAGKWSLFVDCLKHIM
                     LPAITLSVGAMATIARFTRASLLEVLGNEYVRTARAKGLTERSVIFRHALRNSFIPTL
                     TMIGLSFGWAMGGSVLVETVFDWPGIGLYATKSALTLDFMPIVGIALLYGIVFSLINI
                     LVDVSYGFLDPRVTHS"
     gene            complement(2388..2648)
                     /locus_tag="VFQ54_03345"
                     /pseudo
     CDS             complement(2388..2648)
                     /locus_tag="VFQ54_03345"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3037..>3448)
                     /locus_tag="VFQ54_03350"
     CDS             complement(3037..>3448)
                     /locus_tag="VFQ54_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164052.1"
                     /translation="ELNIKVTMTPLVWPDMVARAADPKTSPGMMAVYASADYIDPDNF
                     LWSQYQSGTIGSWANASQYANPDFDKLLVEARGTVDPAKRKDIYDQAQTMLVDQAVEI
                     WVYCEVSNDSWVAQLGPDGLSKVNGDIRVVQYTS"
BASE COUNT          680 a         1100 c         1110 g          558 t
ORIGIN      
        1 cgtgatggtc gcgacatccc tcatcctctg cgaactcctg gcgagcagtc gcaacaccat
       61 catcaccttc ttcgtctgcc actgggccgc ggcgatcggg acgatcctca tgctgcgcct
      121 gctttccgga ctcgggatgg atcgcgcgac ggtcctcctg acgattccgg acgccgggtc
      181 atccgcctcg gttcacggcg cctacgccgt cgccgccatg atgctgccac cccggctcgc
      241 gacgatcgcg tatggcatcc tcctcggcat cacggtcgtt ctgctcttcc agcagtcgct
      301 cgacgccgcg atcgcccacc tgctcgccgt cggcatcggc ggtgcgatcg gcctcctcat
      361 cttccggcct cgaatgatcg caaccttcac gtcgtcgcac gctgcaacag acctggcgac
      421 cgatccgctg caaacggaaa ccgcccgggg cgtttgagcg ccccgggcag ttctggttca
      481 tcgaccgcct gatgcggcgt gttgaatgca gccgttgtgt tcgaggcggg acatagaagt
      541 cccgccgtac tcacgaacga cggccctaga tgcggatatc ccggcggctc cattcgacag
      601 gctcagagcc agcttctgtg gccgcccgtt acttgcgcga gcgcggatcg aagacatcgc
      661 gcagcccgtc acccatgagg ttcgaggcga cgacggtgag caggatcgcg agaccgggga
      721 aggtcgagaa ccaccaggcg tcgcggatgt agttgcgacc atcggagacc atctggcccc
      781 aatccggcgc gggcggttgg ctgccaagcc cgatgaatcc cagggcagcc gtcgtcagca
      841 cggcgaagcc gacgtccatc gaggccttca ccagcacggt cgggaatgca ttgggcagga
      901 tgtggcggaa gagaatccgc atccggctcg caccgagcgc atcagccgct tccacaaact
      961 gggattgccg aagcgagatc acgttcgccc gcacgagacg gcaatacccc ggccaccaga
     1021 cgagcgagat cgcgatcatc accttgaaca accactgacc gagcgcggcg gcgaaggcca
     1081 tcgccaggat caggtcgggg atcgtcagga agatgtcggt gatccgcatg atgatgttac
     1141 cgatccagcc tccgaagaag ccggccagcg caccaagcag cgtcccgatc gagacggcaa
     1201 ggatgatgac gatgaatccc gccagcagcg acgcgcgagc acccacgacg acccgggtga
     1261 ggatgtcgcg tcccgcctga tcggtcccga accagtgatc ccaggaggga gccttcagct
     1321 tgtcgccgaa atggaccgcg ttgctggcgt ccttgggata agggacgaag aagggtccga
     1381 agatcgcgat cagcacgatg gccacgaaga tcgtgagacc cgccaggggc agggggctct
     1441 gccggaactt gcggaaggca cgccgcgtct ccgggctcag ccggatccgg aatccccggc
     1501 ggggctgcgc atagccggat tgggagagac tcgccgctcc tgggtcgctc atggagcctc
     1561 ccggaaaccc cgatcggggc agaggttgat tgcgatcatg aatgcgtcac ccgaggatcg
     1621 aggaagccgt agctgacatc gaccaggata ttgatcaggc tgaagacgat gccgtagagc
     1681 aaggcgatac cgacgatcgg catgaagtcg agcgtgagcg ccgacttcgt cgcgtagaga
     1741 ccgatccccg gccagtcgaa gaccgtctcg acgagaaccg atcctcccat cgcccagccg
     1801 aacgagaggc cgatcatcgt cagggtcggg atgaaggagt tccggagcgc gtggcggaag
     1861 atcacgcttc gctcggtgag ccccttcgcg cgagcggtgc ggacatattc gttgccgaga
     1921 acctcgagca ggctcgctcg cgtgaagcgg gcgatcgtcg ccatcgcgcc gacactgagg
     1981 gtgatcgcgg gcaacatgat gtgcttgaga caatcgacga agagcgacca cttgccggcg
     2041 agcagcgaat cgacgagata gagaccggtc acatgcggcg gcggtatcgt cagcgcctcg
     2101 aggcggccgc tgatcggcag ccagccgagc ttgaggccga ggaagagctg catcaggatc
     2161 gccagccaga atgcgggtac gctcaggaac atcagggaga tgatcagcga gagatggtcc
     2221 gcgagacggt cggcgcgcac agcggcgagg acgccgagcg gaacgccaac ggcgacggcg
     2281 atgaccatcg cgacgagcac gagttcgagt gtcgccggcc agtaggtgcg gagatcatcg
     2341 aagacgggac gccgggagag gatagacgtt ccccaatccc cctggaagag accggtgacg
     2401 tagttccagt actggacggg gatcggcttg tcgagcccga attcctggcg gatctgctct
     2461 tacatctgct gggtcgcctg cggccccgcc gcgagccggg ccggatcgcc tggcacgatg
     2521 tgcgagagcg cgaacgtcag cagcgagaca ccgaagagca ccggaatgat cagcagcagg
     2581 cgccgggtga tgtagcgtgt cagattcatg gcgcctccct tgtcaactgc gaacggcgac
     2641 cgagacatcg ttctcgaagg cgggacacgg aagtcccgcc ctacgaacgc gacgcgtcat
     2701 gcaagccgcc tacacaccga tgctcatctt ttcgagacac ggcaacgatc gaccggaact
     2761 ccacccgttc gcgatcaagc cgagagaccg ttcgtgagta cgggggactt ctatcccccc
     2821 ctcaaatccg acaccacgct gctcacgacc cggcagatgc accacgtagg acgggccttt
     2881 atgtcccgcc gcgaacagaa atgaacgtct ggtagtctgt caccggtatc ttgccggttg
     2941 gcatcgtctg ccaacgatca tatgcggctc cgccgcatgg gccacacggg gtggcccgct
     3001 acggaaccgt cctggcaatt caggcgtgac gcgccactag ctcgtgtact ggaccacgcg
     3061 gatgtcgccg ttcaccttgc tcaggccgtc gggcccgagc tgcgccaccc aggagtcgtt
     3121 ggagacttcg cagtagaccc agatctccac ggcctggtca accagcatcg tctgcgcctg
     3181 gtcgtagatg tccttgcgct tggccgggtc gacggtgcca cgcgcctcga cgagaagctt
     3241 gtcgaaatcc gggttcgcat actgcgacgc gttcgcccac gatccgatcg ttcccgactg
     3301 gtattgcgac cagaggaagt tgtccggatc gatgtagtcg gcggaggcgt agacggccat
     3361 catgcccggc gacgtcttcg gatcggcggc gcgcgcgacc atgtccggcc agacgagcgg
     3421 ggtcatcgtg accttgatgt tcagctcg
//
LOCUS       DASTMD010000224         1638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_646713, whole genome shotgun sequence.
ACCESSION   DASTMD010000224 DASTMD010000000
VERSION     DASTMD010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646713"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VFQ54_03355"
     CDS             complement(<1..1258)
                     /locus_tag="VFQ54_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU0164053.1"
                     /translation="MMIAGPWHAAAELSERAVWPPGALTAADEARFARYVESLSFYHG
                     EQWPAPTRKGTRQLVFNYARALIRKSASYVFPGPVTFSIAPGDIGSAAQAKANEAERL
                     LAGIVQALDLARLDLALAVDAAILGDAAVKVTWDTTRKRPRVVAVDPATLVVRTAADD
                     PRAIASVTQAYGLTGSQIAAMLPDGGTGIADLDPERGYPVVETWTDARWQMQVAGQIV
                     HDGPNPCGWIPYAIATNDPRPFAFWGQSDLTDLVDVCVEINQRMTVLSRVLELSGAPI
                     AVLENVDGSEGISVGPGAKWELPEGAKAYLLDLLEGGGTEVHLSYVAQLFSILHDLSE
                     TPKTAFGDSGRDLSGAALEVEIQPLVQKVARKRRMWDAFFAERNFRLLDLLERFGGHD
                     LGGLRQTTTLWPPVLPSDTDSAVRNAV"
     gene            1432..>1638
                     /locus_tag="VFQ54_03360"
     CDS             1432..>1638
                     /locus_tag="VFQ54_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164054.1"
                     /translation="MPRLFVTLALLILVGVVSPVSSVSAQDDPTMALLARLPGLESAY
                     ARKYMDASEFGVGTPATTGVDDVIV"
BASE COUNT          281 a          516 c          557 g          284 t
ORIGIN      
        1 caaccgcgtt tcgcacggca ctgtcggtat cgcttggaag aacaggtggc cagagcgtgg
       61 tggtctggcg gagaccgccg agatcgtgac cgccgaagcg ttcgagcaga tcgagcaggc
      121 ggaaattgcg ctccgcgaag aaggcatccc acatccgccg cttgcgcgcc actttctgca
      181 cgagtggctg gatctcgacc tcgagcgcgg cgccggaaag atcgcgaccg gaatcgccga
      241 aggcggtctt cggcgtctca ctcagatcgt gaaggatgct gaagagctgg gcgacgtagg
      301 agagatgaac ctcggtgccg ccgccctcca ggagatcgag gaggtaggct ttcgccccct
      361 ccggcaattc ccacttcgcg cccggcccga cgctgatccc ttccgagcca tcgacgtttt
      421 ccaggaccgc gatcggggcg ccggagagtt cgagcacgcg ggagaggacg gtcatccgct
      481 ggttgatctc cacgcagacg tcgacgagat cggtgagatc ggactggccc cagaaggcga
      541 acgggcgggg atcgttcgtc gcgatcgcat agggaatcca tccgcatggg ttcgggccgt
      601 cgtgaacgat ctgtccggcg acctgcatct gccaacgcgc atccgtccac gtctcgacga
      661 ccggatatcc gcgttcggga tcgagatcgg cgatacccgt tccgccatcg ggaagcatcg
      721 ccgcgatctg tgatcccgtc aggccatacg cctgcgtcac ggatgcgatc gcacgcggat
      781 cgtcggcggc cgtgcggacg acgagcgtgg cgggatcgac ggcgacgaca cgcggtcgtt
      841 tccgggtggt atcccaggtc accttcaccg cggcgtcgcc aaggatggcg gcgtcgaccg
      901 cgagcgccag gtcgagccgg gcgagatcga gcgcctggac gatgccggcg agcaggcgtt
      961 ctgcctcatt ggctttcgcc tgcgcggccg atccgatgtc gcccggcgcg atcgagaagg
     1021 tgaccgggcc gggaaagaca tagctggcgc tcttccggat caacgcccgg gcgtaattga
     1081 agacaagctg acgcgttccc ttgcgcgtcg gtgccggcca ctgctcgccg tgatagaagc
     1141 tcagcgattc cacgtagcgg gcgaagcgcg cctcgtccgc cgcggtgagc gcgccgggtg
     1201 gccagacggc gcgctccgac aactcggcgg cggcgtgcca tggtcccgcg atcatcatgg
     1261 tgtgactcca ttcatacgga tatcgtccgg tcgggccgcg gggttggtgc gattaccacc
     1321 gaggaaccgg ctccgttctg ccggcatgca tcatcgagag gcgccgccgg aagagttcac
     1381 gccagggaac catgcgacgg gaacacgcgt ttcctccggg aaggagggga tatgcccaga
     1441 ttgtttgtca cactcgcgct cttgattctc gttggtgtcg tcagtccggt ctcgagtgtg
     1501 tcagcccagg atgatcccac gatggcgctg ctcgcgcggt tgcccgggct ggaatccgcc
     1561 tacgctcgca agtacatgga tgcctccgaa tttggcgtgg ggacacccgc gacgactggc
     1621 gtcgatgacg tcatcgtc
//
LOCUS       DASTMD010000225         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1182179, whole genome shotgun sequence.
ACCESSION   DASTMD010000225 DASTMD010000000
VERSION     DASTMD010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182179"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFQ54_03365"
     CDS             complement(<1..282)
                     /locus_tag="VFQ54_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164055.1"
                     /translation="MTGEAGARDRSATNPDRPGDPFAEYDDFDQEVAENGRIGYSRYG
                     RLSSIGLAIVILIGVIGVAVADRVDNGNSNDSNLDANATAAVEKNRPAPD"
     gene            complement(369..929)
                     /locus_tag="VFQ54_03370"
     CDS             complement(369..929)
                     /locus_tag="VFQ54_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164056.1"
                     /translation="MNRRASAVAQRTAAPSQRRKQRGPHILSTLLFIAAIGFAVAAGY
                     LYLQDRDHTENRVPPTALPGRNDLGAVLQQFKDAGLSAGYGRITGHAEGIDEPGQSLT
                     VDGQTVLVFIYNDADKASSVKDRKAAAATLPDDLDGVAITSPSGTDLRKGQPIYMAQG
                     SNIITIMIGGDADLASKVQTVVEALP"
     gene            1356..1532
                     /locus_tag="VFQ54_03375"
     CDS             1356..1532
                     /locus_tag="VFQ54_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164057.1"
                     /translation="MSTLRRILSHANFLNNNSRFERYYSGVLQSGQGFPTADEARKDL
                     NQYDRAYMPYGWPR"
BASE COUNT          396 a          647 c          663 g          402 t
ORIGIN      
        1 gtcgggagcg gggcggttct tctcgacggc ggccgtcgca ttcgcgtcga gattggagtc
       61 gttgctgttg ccgttgtcga cacgatcggc gacggcaact ccgatcaccc cgatgaggat
      121 gacgattgcg agtccgatcg aggagagccg accgtagcgg ctgtagccga tccggccgtt
      181 ctcggcgact tcctgatcga agtcgtcgta ctcggcgaag ggatcgcccg gccggtccgg
      241 attcgtggcg gaacggtccc tcgcaccggc ttcgccggtc accgcgggcg gagtggagct
      301 gtcccccggc tcgggctccg aaggcgtctg gccggggatg tcgtcagatt gcgctggggg
      361 tagctggctc aggggagtgc ctcgacaacg gtttgaactt tggatgccag atctgcatcg
      421 ccgccgatca tgatcgtgat gatattgctg ccttgcgcca tgtagatcgg ttggcccttg
      481 cgaagatccg tccctgaggg ggaggtaatc gcgacgccat ccagatcatc cggcagcgtg
      541 gcagcggcgg ctttccgatc cttcaccgac gacgccttgt cggcgtcatt gtagatgaag
      601 acgagaacgg tctggccgtc gacggtgagc gactgcccag gttcatcaat gccctcggcg
      661 tggccggtga ttcgcccgta cccggcgctg agaccagcgt ccttgaactg ctggaggacg
      721 gccccgaggt cattgcgacc ggggagggcc gttggcggca cgcgattctc ggtgtgatcg
      781 cgatcctgaa ggtagagata tcccgcggcg acggcgaacc cgatcgcggc gatgaagagg
      841 agcgtgctga ggatgtgcgg gccgcgctgc ttgcgccgct gggaaggggc ggccgtgcgc
      901 tgggctactg cgctagctct tctgttcata cggtccgatc catcgtgcca accgacaaca
      961 ccgtcatcta ggtcatctcc ggggagcgat cgtcatcaac tgcgatcgcc tctgctgctt
     1021 gtggatgccg cgcggcatcc cgtctcgtcc gatctctccc acggatcggt ccaggcggcg
     1081 tgcacgtccc ctgcgaaaca cgcaggaagc accgtgccca ctcacgaagc ccccagccac
     1141 gcagatggta cgtcttttgg gtgaccggcg accagcagtc accgccagac atgggggaat
     1201 atccatgttt cgccatcaga tcacgatatc ttgatatcat gttgatatcg attcaacaat
     1261 tgatgcacat atctcgatag tatgatatac tgtcgtcagt gaggtgaccg atggggttca
     1321 ccagcagcga cgatgcacag aaaggtcaaa catccatgag cacgcttcgc cggattcttt
     1381 cgcacgcgaa cttcctcaac aacaacagcc gtttcgagcg ctactacagt ggcgttctcc
     1441 agagtggtca gggcttcccg accgcagacg aggcccgcaa ggacctcaac cagtacgacc
     1501 gcgcgtacat gccgtacggt tggcctcgct aatcaccacg aaggcacgat cgcggcatcg
     1561 gcgtcccgct acgtggtgaa ccaggcgtca aggtcagaga attgttcccg agctgaaact
     1621 gtcgaataag cttcgcccga caggcagttc cagactcggt cgataatgcg taagcccccg
     1681 gatggaaatg tcccatccgg gggctttttg cgctgttcca tgctcgtcga gtgatcatgc
     1741 ccgtcgccgc atgctccccc aacagcccct ccccgatccg tcatcctgaa cagagtgaag
     1801 gaccccaacg ccaacgattg atacgatgtc cctccgcgaa cctgggtcga tcgcgacgac
     1861 ccaaaacggc gacgagcgcc gtggggacgg gatgggaacg cgttgcccgg catcctggat
     1921 gcgacggcca gggcgatctc ctgacgatcg cccccacgga atcaactcag cgaacgtcga
     1981 gtgcgagttc atggagcagg gcgtcatccg gcgcatggaa gcgctccaac ggcaacgtgg
     2041 tcgaggcgtt gggaacgatc acgaggcgtg catcgggtcc gtgccgctcg atcgcggcat
     2101 cgatcgcc
//
LOCUS       DASTMD010000226         1635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_110282, whole genome shotgun sequence.
ACCESSION   DASTMD010000226 DASTMD010000000
VERSION     DASTMD010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110282"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..564)
                     /locus_tag="VFQ54_03380"
     CDS             complement(265..564)
                     /locus_tag="VFQ54_03380"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU0164058.1"
                     /translation="MSEYEVLPRAAEDLFLAVQYTRDRWGDAQVDRLQNQFDRVIESL
                     SEFPEIGRSRSDLLPGLRSMPVFRFHLLYVVENGVIVVVRFVHQKRNLTTLMFEE"
     gene            complement(561..938)
                     /locus_tag="VFQ54_03385"
     CDS             complement(561..938)
                     /locus_tag="VFQ54_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEU0164059.1"
                     /translation="MNVNLTPYWEKYIQEKIASGRYNNASEVIREALRNMEYVEEVKE
                     FRAYAKDALAPEHRDQRVKAGPNFMADIVERVRSGERATGSLPRYLTPPEGVEEALDL
                     DEDDEAVMRVAEAGGQYRADSSQ"
     gene            complement(978..>1635)
                     /locus_tag="VFQ54_03390"
     CDS             complement(978..>1635)
                     /locus_tag="VFQ54_03390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164060.1"
                     /translation="NAPERREQRTTPRTATVDDTPRPGVIALVRSSRRFQRLIVTTAP
                     WVFGAATVSMTVLPHLVADRVAGNELAFAGIITGLTQLTGVAVQPWAQRRERKGKRDA
                     LTIGMLMAAGGFLLSIVVASSGNPWWVVIAAPIFGVGFGVLLVSGIDEITRMADPRDL
                     GGFVALFYVLTYLGFALPILLAAILPAIDYQVSFAICAVLVLLSAPVVRQELARDSNH
                     "
BASE COUNT          323 a          507 c          485 g          320 t
ORIGIN      
        1 ttctgcgact gcgtatcatg gcccattgga atgcatccta tcgcgatgca ccgacaaacc
       61 atagaatgct caaccctgcc gcgcaagcac ccggacgtaa aacgatggtt atgtctgcgc
      121 attttccatg gataacggtt accgacgacc ggggaagcgt gtccgtcgga accgcgtcac
      181 ccgcgcgatt gcccagagat acgtgatgag gatcgccgcc atgaagatcc cgattcctgt
      241 cagggtgcga gtaacaacag tgcctcactc ctcgaacatc aaggtcgtca gattgcgctt
      301 ctgatgaacg aagcgcacga cgacgattac accattctcg acgacgtaga ggaggtggaa
      361 gcggaaaacc ggcatggatc gcagcccggg caggaggtcc gatcgtgatc gcccgatttc
      421 agggaactcg gagaggcttt caatgactcg atcgaactgg ttctgcaatc gatcgacctg
      481 agcatcgccc catcgatccc gtgtgtattg cacggccaga aagaggtcct ctgcggctcg
      541 cggcagaacc tcatactcac tcattgcgaa gaatcggcgc gatattggcc gccggcttca
      601 gcgaccctca taaccgcctc gtcgtcttcg tcgaggtcaa gcgcttcctc gacgccttca
      661 ggcggtgtca gataacgtgg aagtgatcct gtcgctcgct cgccacttcg gactcgttcg
      721 acaatgtcgg ccatgaaatt ggggccagcc ttgactcgct ggtcgcgatg ctccggtgcc
      781 agggcatcct ttgcgtaggc gcggaattcc ttgacctcct ccacatactc catgtttcgc
      841 agcgcctcgc ggatgacctc gctggcgttg ttgtagcgac cgctcgcgat cttttcctgg
      901 atgtactttt cccagtaggg ggtgagattg acgttcatcg gcgtcgctcc tttcactgcc
      961 catcctagcg gtcattgtca atgattgcta tctcttgcca attcctgccg aaccaccggc
     1021 gcgctgagga ggacgagcac cgcacagatc gcgaagctga cctgatagtc gatggcgggc
     1081 agaatcgcgg ccagaaggat cggaagtgcg aacccgaggt aggtgaggac gtagaagagc
     1141 gcgacgaagc caccaaggtc gcgtgggtcc gccatccggg tgatctcatc gatgcccgag
     1201 acgagaagaa cgccgaatcc cacgccgaag atcggcgccg cgatcaccac ccaccacgga
     1261 ttgccgctgg atgcgacgac gatgctgagc aggaatccgc ccgccgccat cagcattccg
     1321 atcgtcagtg cgtctcgctt ccccttccgc tcgcgtcgct gcgcccatgg ctgcacggcg
     1381 actccggtca actgcgtcag tccggtgatg atgccggcga aggcgagttc gttcccggcg
     1441 acacgatcgg cgacgagatg gggaaggacc gtcatcgaaa cggtcgctgc gccgaagacc
     1501 cacggtgcgg ttgtcacgat caggcgctgg aaccgccgcg atgagcgcac cagggcgatg
     1561 acgcccggtc gcggcgtgtc atcgaccgtt gcggtgcgtg gcgtcgtcct ctgctcccgg
     1621 cgctccggcg cgttc
//
LOCUS       DASTMD010000227         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_200980, whole genome shotgun sequence.
ACCESSION   DASTMD010000227 DASTMD010000000
VERSION     DASTMD010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200980"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFQ54_03395"
     CDS             complement(<1..263)
                     /locus_tag="VFQ54_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164061.1"
                     /translation="MTDSSTSSPVRIVLDPQESLPDLLARVRELSGRSVVIEILDHSP
                     ILLTATEFRTLGEAASRANIDLTLLTEDRLRIQLANMFKLIKAG"
     gene            complement(365..>2984)
                     /gene="alaS"
                     /locus_tag="VFQ54_03400"
     CDS             complement(365..>2984)
                     /gene="alaS"
                     /locus_tag="VFQ54_03400"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU0164062.1"
                     /translation="SNADIRRKFIEFFESKQHLHYPSSSLVPVNDKTVLLTTAGMQQM
                     VPYFLGLETPPRERMCTVQKCFRTVDIDEVGDESHCTFFFMLGNFSIRDYFKSSSLAW
                     SWEFLTEWMNIPADRLYPTIHPDDDEAYAIWRDEIGVPEDRIYKLEDNWWRAGPTGPN
                     GPDSEIYFDWGEHLQPDAKPGDDGPRFLEVWNNVFMQFNTTPDGVSSLMPKPSVDTGM
                     GLERLVQVVQGGKSLYDTDLYQPIIQRAAMLAGKAYGIDPQLDRSLRIIADHIRGSVF
                     LIGDGVLPGNEGRSYVLRRVLRKAIRHGRMLGIEGAFLGEMATIVIDEFGGQYPELVE
                     RRNQILRVLSYEEASFGKTLTSGMNRLQALTAELKANGETVIPGEEVFRLYDTYGFPY
                     DLTVELAQEEELTVDTDGFETAMAHQRVQSRSLDLFKDTSRDRANLYAGIQPRTTFLG
                     YEEERGEATVVALLGIDGLLEQAEAGQEIEIVLDRTPFYGESGGQVGDSGQIRTETGL
                     IDIDDTRKPAPDLFVHRGTVAEGFVRIGDSAVAKIDGYRRQQIRRNHTATHLLHKALR
                     TVIGTDTHQAGSLVAPDRLRFDFTSLDAIDAEQVREVAAIVNTEIANDRPVETRVMAQ
                     KDAIAEGAMALFGEKYGETVRVVQIDDYSKELCGGTHVKRTGEIGPFVITAEGSVASG
                     VRRIEALTGQAAIERILGQQQLVEDLGRDLRVTWTEVPTQVRALSERVRMQEREIARL
                     QGEVAGAASADLLQRVVQVGGDVPLLAAEVTVENKQGLRQLGDRLRDSLQSGVIVLGT
                     VVDDAPSLLVMVTPDIVARGVKAGALIGAIAPHIDGRGGGRPELAEAGGRNASGLATA
                     IASVPDALSGLVVQPA"
BASE COUNT          508 a          992 c          952 g          532 t
ORIGIN      
        1 ccggccttga tcagcttgaa catgttggca agctgaatcc gcagccgatc ctcggtcagc
       61 agcgtgagat cgatattcgc gcgcgacgcc gcctccccga gcgtccggaa ctccgtcgcc
      121 gtgagcagga tcgggctgtg atcgagaatc tcgatcacca ccgagcgacc gctcagttcg
      181 cgaacgcgcg ccagaagatc gggcagcgat tcctggggat cgagcacgat cctgactggg
      241 gatgaggtcg acgaatcagt catgaagcga cactatccgg gcacgaacct cgcgccgtaa
      301 tcagtgattc cgggagaggt tcggcgaatg aggtcgcgag agattccgca acctccacga
      361 ctcctcaggc aggctggacg acgagaccgg agagggcatc cgggacggac gcgatcgcgg
      421 tcgcgagacc ggacgcgttc ctgcccccgg cctccgcgag ttccggccgt cccccgccgc
      481 gaccatcgat atgtggcgcg atcgccccga tcagtgcgcc agccttcaca ccccgcgcga
      541 cgatatcggg cgtcaccatc acgagcaggc tcggcgcatc gtcgacgacg gtgccgagca
      601 cgatcacacc cgactgcagg gaatcgcgca gccggtcccc gagttgacgc aggccctgct
      661 tgttctccac cgtcacctcg gcggcaagga gcggaacgtc gccaccaacc tgaacgaccc
      721 gctgcagcag gtccgccgac gccgcgcccg cgacttcccc ttgcaggcgg gcgatctcgc
      781 gctcctgcat ccgcacacgc tcgctgagcg ccctgacctg ggtcggaacc tccgtccatg
      841 tcacgcgcag atcgcgcccg agatcctcga cgagttgttg ctgcccgaga atccgctcga
      901 tcgctgcctg accggtcagc gcctcgatcc ggcggacacc ggacgccaca cttccctcgg
      961 cggtgatcac gaagggaccg atctcgccgg tccgcttgac gtgggtgcca ccgcagagtt
     1021 ccttcgagta gtcgtcgatc tggacgacgc gaacggtctc gccgtacttc tcaccgaaga
     1081 gcgccatcgc cccctccgcg atcgcgtcct tctgcgccat cacccgcgtc tcgaccggcc
     1141 ggtcgttcgc gatctcggta ttgacgatcg cggcaacctc gcgcacctgc tccgcgtcga
     1201 tcgcatcgag cgacgtgaag tcgaagcgaa ggcgatccgg cgcgacgagc gatcccgcct
     1261 ggtgcgtgtc ggtaccgatc acggtgcgca gcgccttgtg caggaggtgc gtcgccgtgt
     1321 gattgcgccg gatctgctgg cggcgatagc catcgatctt cgcgaccgcc gagtctccga
     1381 tgcggacgaa accctccgcc acggttcccc ggtgcacgaa gagatcgggc gccggcttgc
     1441 gggtgtcatc gatatcgatg aggccggttt cggtccggat ctggccgctg tcgccgacct
     1501 gaccgccgga ttcgccgtag aacggcgtcc ggtcgagcac gatctcgatc tcctgccctg
     1561 cctcggcctg ctcgagcagc ccgtcgattc cgaggagcgc gacgaccgtc gcctcgcctc
     1621 gctcttcctc gtagccgagg aaggtcgtgc gcggctggat ccccgcatac aggttcgcgc
     1681 gatcgcggga ggtgtccttg aagagatcga gactgcggct ctgcacgcgc tggtgcgcca
     1741 tcgccgtctc gaagccgtcg gtatcgaccg tcagctcttc ttcctgcgcc agctctacgg
     1801 tgaggtcgta gggaaagccg taggtgtcat agaggcggaa gacctcttcg ccggggatga
     1861 cggtctcacc gttggccttc agctccgccg tcagcgcctg caggcggttc atgccggagg
     1921 tgagcgtctt gccgaagctc gcctcttcat aggagagaac ccgcaggatc tggttgcggc
     1981 gctcgaccag ttccggatac tggccaccga attcatcgat cacgatcgtc gccatctcgc
     2041 caaggaaggc gccctcgatc ccgagcatgc gaccgtgacg gatcgccttg cggagcacgc
     2101 gccggaggac gtaggagcgg ccctcgttgc ccgggagcac gccgtcgccg atcaggaaga
     2161 cggagccgcg gatgtggtcg gcgatgatcc ggagcgatcg atcgagctgg ggatcgatcc
     2221 catatgcctt gccggcgagc atcgccgcgc gctggatgat cggctggtag agatcggtat
     2281 cgtagaggct cttgccccct tgcacgacct gcaccaggcg ctcgagcccc atgccggtgt
     2341 cgacgctcgg cttcggcatc agcgaggaga cgccgtccgg cgtggtgttg aactgcatga
     2401 agacgttgtt ccagacctcg aggaagcgcg gtccgtcatc gcccggcttc gcatccggct
     2461 ggagatgctc gccccagtcg aaatagatct cggaatccgg tccgttcggg ccggttggcc
     2521 ctgctcgcca ccagttgtcc tcaagcttgt agatgcgatc ttccggcacc ccgatctcgt
     2581 cgcgccagat cgcgtacgcc tcgtcgtcgt ccgggtgaat cgtcggatac aggcgatccg
     2641 ccgggatgtt catccactcg gtgaggaact cccacgacca ggcgagcgag ctgctcttga
     2701 agtagtcacg gatcgagaag ttgccgagca tgaagaagaa ggtgcagtga ctctcgtcgc
     2761 cgacctcgtc gatgtcgacg gtgcggaagc acttctggac ggtgcacatc cgctcgcgcg
     2821 gcggtgtctc gagacccagg aagtagggaa ccatctgctg catgccggca gtggtgagga
     2881 gcaccgtctt gtcgtttacc ggcaccagcg aggagcttgg gtaatggaga tgctgcttcg
     2941 attcgaagaa ctcgatgaac ttccggcgaa tgtccgcgtt ggac
//
LOCUS       DASTMD010000228         7611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2075379, whole genome shotgun sequence.
ACCESSION   DASTMD010000228 DASTMD010000000
VERSION     DASTMD010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075379"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VFQ54_03405"
     CDS             complement(<1..883)
                     /locus_tag="VFQ54_03405"
                     /inference="COORDINATES: protein motif:HMM:NF017039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU0164063.1"
                     /translation="MASTSSVRRSAAAAPENDIVPLRKQYLQIKARYPDTVLFFRLGD
                     FYETFDGDAQIATEVLDIVLTGREMGKNLRVPMAGIPYHAAEGYIARLIAAGHKVAIC
                     EQIGEPTKGKGLVERDVTRVVTPGTVVDPSMLDAKTNNYIVAVAIDRNRAGISYADIT
                     TGEFATTEIIEQTAEEAILAAGRELLRLRAAEIVLAADLTDAQPLPMSSWLPEAVSLS
                     RTEAWRWTVDRGAEAIMRHFDVDSLDGFGVSGKPLAIRAAGGLLQYLEETQISGLQQV
                     TALSTYAIDSFMTLDAQT"
     gene            1087..1365
                     /locus_tag="VFQ54_03410"
     CDS             1087..1365
                     /locus_tag="VFQ54_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0164064.1"
                     /translation="MALFAVDLSLNSDLDRRGEVRPEHREYLRSLVDAGKLHESGPYL
                     DNSGALLIYIAESVADVQEILAADPYTKAGLVEGAKIREWNIVISKNA"
     gene            complement(1457..2467)
                     /locus_tag="VFQ54_03415"
     CDS             complement(1457..2467)
                     /locus_tag="VFQ54_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0164065.1"
                     /translation="MSDRMATYDVSQIDKLASDDLNGNDKIEVPEEITTQMADEIEPE
                     VDLERDLDVLQRLDPNFINDPVRLYLREIAETPLLTHAQEIDLAKRVENGDLQAVQQF
                     VRANLRLVVSIAKRYVNRGLTLLDLIQEGNIGLMRAVKKYDWRKGFRFSTYATWWIRQ
                     AITRAIADQSRTIRLPVHMGDSISRYRKTLNLLAQELGRQPTPEEVAEAMAVAPEKIH
                     QIVQAAQRTISLETPIGNEDDTSLGDLIADEVSETPYEAASESMLKRDVNAALDTLSA
                     REKLVLQLRFGLGQGHQHTLAEVGEQLQISRERVRQIENEALQKLRKLDGDRLFAYHQ
                     EL"
     gene            complement(2882..3250)
                     /gene="erpA"
                     /locus_tag="VFQ54_03420"
     CDS             complement(2882..3250)
                     /gene="erpA"
                     /locus_tag="VFQ54_03420"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEU0164066.1"
                     /translation="MITMTNEAVEQLRDFLVEQGTPDHALRVFVAPGGCSGLQYGMTI
                     DETSDEGDEIIETMGVRVLVDNFSAMYLEGAEIDYVNSLMGGGFTVRNPNAVASCSCG
                     HSFDTGENAQTSQGCGCGSH"
     gene            3456..4490
                     /gene="rsgA"
                     /locus_tag="VFQ54_03425"
     CDS             3456..4490
                     /gene="rsgA"
                     /locus_tag="VFQ54_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU0164067.1"
                     /translation="MPSISRNRPTRAQTNTDAQRNQSLVDVTGTVGPPAGSERGLVMR
                     AHGLWYEVLIGAGEEQRVVLATIRGQLKRRQRRTDIVAVGDRVHVSLLPDNEAVIEYV
                     EPRSRALVRTARHTRDTDQVILANPDQVLFVFATKDPTPHPRMLDRFLILAELQRIPV
                     WIAIGKMDLDDPLDSARVRFRDYERIYPVSYVSVSSGEGVPQLREAMAGKVTVVAGPS
                     GVGKSSLLNLLDPENQRDVGEISVATGKGRHTTVGSRLHRIGDGTFVADTPGMRSLAM
                     HAVTPDRLPACYREFLPYLENCFYQDCTHVHEPGCDVIAAVNDGAISRARYESYVALR
                     TGEEMMEGGW"
     gene            4490..4840
                     /locus_tag="VFQ54_03430"
     CDS             4490..4840
                     /locus_tag="VFQ54_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164068.1"
                     /translation="MARRRDHDTGPNGITVDDLIDGLFPIVDNLLSRVEGDEFTTNDF
                     IDLMLAVPEGVAAYEQAIVHWGEGERHSKMVVHGQAIPGAMRRSKRVEWTGYAHGEND
                     EFGVPAWWRLTGVT"
     gene            5206..7131
                     /gene="ftsH"
                     /locus_tag="VFQ54_03435"
     CDS             5206..7131
                     /gene="ftsH"
                     /locus_tag="VFQ54_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU0164069.1"
                     /translation="MATLIIALVGWYIWEFFGPSQSDGNLSVPYSVITQQVSDKNVKE
                     ATLSGTKITVKLDSQVAYDKDTEKVVADAPSNDQISRSSKLEATLPQGIQNDQLLSQL
                     EGSGAVIKGEVNSGSFWTSLLISFLPFLLFIGLIVFMGRQMSRGQQNVFGFGRSKARQ
                     NDPERPQVTFGDVAGEDEAKQELMEVVDFLRNPTKYHALGARLPRGVLLVGPPGTGKT
                     LTARAVAGEAGVPFFSVSASEFVEMFVGVGASRVRDLFDRAKAAAPAIIFVDEMDAVG
                     RQRFAGLGGSNDEREQTLNQLLVEMDGFETNQEVIVMAATNRPDVLDPALLRPGRFDR
                     QVTVGLPDRTGRVAILAIHTRGIPLSSKVDLEHIARSTTGFSGADLANLVNEAALTAA
                     RRNRKEILPADFDEALDKILLGVAKSGLTNPKEREVVAYHEAGHALVAHFTPGSDPLR
                     KVSIVPRGNAGGVTIQMPDEDRGLYSRTYLLGRLAGMLGGRAAEQVVFGEITTGAEND
                     LKQATSLARRMVGVWGMSEDVGPIYLGTGEEHVFLGREITQDKSFSDATAQRLDVAVH
                     GIVDNALKQAITLNNLHRDKLEALVAALLERETLDGPEVVAIFGAPPVPEDPEAGMTP
                     RQPVTPDAVFGGGSVAD"
     gene            7378..>7611
                     /locus_tag="VFQ54_03440"
     CDS             7378..>7611
                     /locus_tag="VFQ54_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164070.1"
                     /translation="MIEQRVDDLPDSNLIVAEPANGVEEVVVAAPGPDLPELVPEVYI
                     NRELSWLEFNRRVLREVAETPTPLIERAKFAAIF"
BASE COUNT         1407 a         2326 c         2461 g         1417 t
ORIGIN      
        1 gggtctgggc atcgagcgtc atgaacgaat cgatcgcgta ggtcgacagc gcggtgacct
       61 gctgcagacc ggagatctgc gtctcttcca gatactgcag caagcccccg gcggcgcgaa
      121 tcgcgagcgg cttgcctgag acgccgaagc catcgagcga gtcgacgtcg aagtggcgca
      181 tgatcgcctc ggcaccacga tcgaccgtcc agcgccatgc ctcggtccgg ctgagactga
      241 cggcttcggg aagccaggat gacatgggga gcggctgggc atcggtgaga tcggcggcaa
      301 gcacgatctc ggcggcgcgg agccggagaa gctcgcgacc ggcggcgagg atcgcttcct
      361 ccgcggtctg ctcgatgatc tcggtcgtcg cgaactcgcc ggtggtgatg tcggcgtagg
      421 agatgccggc ccggttgcga tcgatcgcga cggccacgat gtagttgttg gtctttgcat
      481 cgagcatcga tgggtcgacg acggtgcccg gcgtgacgac acgggtcacg tcccgctcga
      541 cgagtccctt ccccttcgtc ggctcgccga tctgctcgca aatcgcgacc ttgtgtcccg
      601 ccgcgatcag gcgggcgatg tagccctcgg cggcatggta gggaatgccc gccattggaa
      661 cgcgcagatt cttgcccatc tcgcgcccgg tgaggacgat gtcgagcacc tcggtcgcga
      721 tctgggcgtc gccgtcgaac gtctcgtaga agtcgccgag gcggaagaag aggaccgtgt
      781 cgggatagcg cgccttgatc tgcaggtact gcttgcgcag ggggacgatg tcattttcgg
      841 gagcggctgc agctgaacgg cggacggacg aggtactggc catatcatct cccgatgatg
      901 ggatgagcgc gcccggagag ccacgggcgg atgttcttta cgtacacagc ataccgtgag
      961 ttggcgaggg atttccggac gatcgatagg ctgccggcgc cggggagacg atggtatcgt
     1021 atggcgttcg tccgcgcgct tgaggcggat atcgaaatcg atgcacacag ggaatggaga
     1081 gcctgcatgg cgcttttcgc ggtggatctc agtctgaatt cggatctcga ccggcgtggt
     1141 gaggtgcgac ccgagcaccg cgaatatctc cgctcgttgg tcgatgccgg caagctccac
     1201 gagtccggac cctatctcga taacagtggc gcgctgctga tctacatcgc cgagagtgtc
     1261 gccgacgtgc aggaaatcct ggctgcggat ccatacacga aggccgggct ggtcgagggc
     1321 gcgaagatcc gcgaatggaa catcgtcatc tcgaagaacg cctgatcggc gcgccgttct
     1381 gacgcggcga tgtccaggaa cagaaacggg ggacggcaat cgccgtcccc cggactgtca
     1441 tgcctgattg cggaacctag agttcctggt gatacgcgaa gaggcggtca ccatcgagct
     1501 tgcgcagctt ctgcagcgcc tcgttctcga tctgtcgcac gcgttcgcgg ctgatctgga
     1561 gctgctcgcc gacttccgcc agcgtgtgct gatgaccctg accgagtccg aagcgaagct
     1621 ggaggaccag cttctcgcgg gccgagaggg tgtcgagcgc ggcgttgacg tcccgcttga
     1681 gcatcgactc gctggccgct tcgtacggcg tctcggaaac ctcgtccgcg atcaggtcgc
     1741 cgaggctggt gtcatcctcg ttgccgatcg gggtctcgag ggagatcgtg cgctgcgcag
     1801 cctggacgat ctggtggatc ttctccgggg cgacggccat cgcctcggca acttcctccg
     1861 gcgtcggctg gcgaccgagc tcctgcgcga gcaggttcag ggtcttgcgg tagcgggaga
     1921 tcgaatcgcc catgtggacc gggagccgga tcgtgcggct ctggtcggcg atggcgcggg
     1981 tgatcgcctg gcgaatccac caggtcgcgt acgtgctgaa gcggaagccc ttccgccagt
     2041 cgtacttctt gacggcgcgc atcaggccga tgttgccctc ctggatcagg tcgagcagcg
     2101 tcagaccgcg gttcacgtac cgcttcgcga tcgagacgac gaggcggaga ttggcgcgca
     2161 cgaactgctg gacggcctgg aggtcaccgt tctcgacgcg cttggcgaga tcgatttcct
     2221 gggcgtgcgt cagcagcggg gtctccgcga tttcccgaag atagagacga actggatcat
     2281 tgatgaaatt gggatcgaga cgctggagca catccaggtc tcgttcgaga tcgacttcgg
     2341 gctcgatctc gtccgccatt tgtgtggtaa tctcttcagg aacctcgatc ttgtcattgc
     2401 cattcaaatc gtcgctggca agcttatcga tctgcgagac atcataggtc gccatacggt
     2461 cgctcatttg cgtaaactcc ttaacattgc gtcggttcga cacccagacg ccacgtgagt
     2521 gacgccgggt ctctcatcag gaacgcgcag atagcgttcc acgacgtgag tggtgcctga
     2581 ccggtcactc taatctacgc cagaatccga catacggatt tctgaccatt cgactgtgcc
     2641 gccatgcggc gaacggaatc catcatcggg gtcgtgggcc atgatgccgc ccacggaacg
     2701 ccggcaggta tcaccggtgg gaccggcgca gcctttcaag tttcgcattg tatcgcgata
     2761 tgtggccact gtaaaccccg ttgacggatt ggcaactcac tgcagaaagc acagaaccgg
     2821 atcgcgccgc ggaagggttc cgcacgcgat ccggtcaggt gagttgccgg gacggcaagg
     2881 cctagtggct gccgcagcca caaccctgcg aggtctgcgc gttctcgccg gtatcgaagg
     2941 agtggccgca ggagcaggag gccacggcgt tcgggttgcg aaccgtgaag ccaccgccca
     3001 tgagggagtt gacgtagtcg atctcggcgc cctcgaggta catggcgctg aagttatcga
     3061 caagaacgcg aacgcccatc gtctcgatga tttcgtcgcc ctcgtcggag gtctcgtcga
     3121 tggtcatgcc atactggaga ccggagcatc caccgggagc aacgaagacg cgaagcgcgt
     3181 gatcgggcgt gccctgctca accaggaaat cgcgaagctg ctcgacggcc tcattggtca
     3241 tcgtaatcat gtcttggtgc tccctcgcac ggaatcatat ggcgcgaagc gagtggatcc
     3301 ggatgcaatc cacccaccta cctctcgtaa ggatagtcgt tccacccggt gtgtcaagat
     3361 actgtgaacg aagattcatg cccctgtata cacttcactc gatcgcgccg ttcgacgatg
     3421 ccgattctta ccccatggga cagcaggaaa cacatttgcc ttcgatctcg cgaaatcgcc
     3481 cgacgcgggc ccagacgaat accgacgcac agcgcaatca gagcctggtc gatgtgacgg
     3541 ggacggtcgg cccacccgcc gggagcgaac ggggtctcgt gatgcgtgcc cacgggctct
     3601 ggtacgaagt gctgatcggt gcgggcgagg agcagcgggt cgtcctcgcg acgattcgcg
     3661 gacagctcaa gcgtcgccag cggcggacgg atatcgtcgc cgttggcgat cgggtccatg
     3721 tctcgctgct gccggataac gaggcggtga tcgagtatgt ggaaccccgg agccgggcgc
     3781 tcgtcagaac cgcgcgccac acgcgggata ccgatcaggt catcctcgcg aatccggacc
     3841 aggtgctctt cgtcttcgcg acgaaggatc cgacgccgca tccacgcatg ctcgatcgct
     3901 tcctgatcct cgccgagctg caacggattc ccgtgtggat cgcgatcggc aagatggatc
     3961 tcgacgatcc gctcgacagt gcccgcgtcc gcttccgcga ctacgagcgc atctatcccg
     4021 tttcctatgt aagtgtgagt tccggtgagg gagtgccgca gcttcgcgag gcgatggccg
     4081 ggaaagtgac ggtcgtggcg ggaccgtccg gcgtcgggaa gtcgagtctc ctcaatctcc
     4141 tcgatccgga gaaccagcga gatgtcggag agatctcggt tgccacgggc aagggccgcc
     4201 acaccaccgt cggctcccgg ctccatcgca ttggcgacgg cacgttcgtc gcggacacgc
     4261 cggggatgcg ctcgctcgcc atgcacgcgg ttacgccgga ccggcttccg gcgtgttacc
     4321 gggagtttct tccctatctc gagaactgct tctatcagga ttgcacgcac gtgcacgaac
     4381 ccggctgcga cgtgatcgcc gccgtgaatg atggcgcgat ctcgcgggcc cgctacgaga
     4441 gctacgtcgc actgagaaca ggtgaggaaa tgatggaagg gggttggtaa tggcacggcg
     4501 ccgcgatcac gacaccggtc cgaacgggat caccgtcgat gatctgatcg acggactctt
     4561 tcccatcgtc gacaatttgc tctcgcgagt cgagggcgat gagttcacga ccaacgactt
     4621 catcgacctg atgctcgcgg tgcccgaggg cgtcgccgcc tacgagcagg cgatcgttca
     4681 ctggggcgaa ggcgagcgac acagcaagat ggtcgtccac ggccaggcga ttccgggggc
     4741 gatgcgacga tcgaagcgcg tggagtggac gggatacgcc cacggcgaga atgacgagtt
     4801 tggcgtgcca gcctggtggc gactcaccgg tgtgacataa tcgctccata ccagttcgat
     4861 ggagaactgg agcaaggtcg ggtggtgatt cgcaattaat ctctccctga tcgaatgatt
     4921 gggtgctttg gttgatatct gtgaactaag ctttgtctat actgggagat gaagctcgaa
     4981 acagtcattc atcccttcga gatcatcgtc ccggactggt tgagacaacg ctcatcgcgc
     5041 aagaaagaca gacgcaaaat gcaacccaag gatcctaaga gaccccagtt gcccggcccg
     5101 gacaggaacg gtaaccagca gcgcaaccaa cagaatggtg gcaatggtgg aaagggaggg
     5161 ccgcagtctc cactgcagcg tgctccccgc attccgacat ggatcgtggc gacgctcatc
     5221 attgccctcg tcgggtggta catctgggaa ttcttcggtc ccagccagag cgatggcaat
     5281 ctcagcgttc cgtactccgt catcacccaa caggtcagcg acaagaacgt caaggaagcg
     5341 acactctccg gtaccaagat cacggtcaag ctcgacagcc aggtcgccta tgacaaggac
     5401 acggagaagg tcgtcgccga cgccccgtcg aacgatcaga tttcccgcag ttccaagctc
     5461 gaggccacac tcccgcaggg aatccagaac gatcagcttc tgagccagct ggaaggctcg
     5521 ggtgcggtga tcaagggtga ggtcaactcc ggaagcttct ggaccagtct cctgatcagc
     5581 ttcctgccgt tcctcctctt catcggactc atcgtcttca tgggacggca gatgagccgg
     5641 ggccagcaga atgtcttcgg cttcggtcgg agcaaggcca ggcagaacga tccggagcgg
     5701 ccgcaggtca cgttcggcga cgttgccggc gaggacgagg ccaagcagga gttgatggaa
     5761 gtcgtcgact tcctccgcaa cccgaccaag taccacgcgc ttggcgcgcg cctcccgcgc
     5821 ggtgtccttc tcgtgggccc tccgggtacc ggtaagacgc tgaccgcccg cgccgtcgcc
     5881 ggtgaggctg gagttccgtt cttcagcgtc tccgcctccg agttcgtcga gatgttcgtt
     5941 ggtgtcggtg cgagccgtgt ccgcgacctc ttcgatcggg cgaaggccgc ggctcccgcg
     6001 atcatcttcg tcgacgagat ggacgccgtc ggtcgccagc gcttcgccgg cctcggtggt
     6061 tccaacgacg agcgcgagca gacgctgaac cagctgctcg tcgagatgga cggattcgag
     6121 acgaaccagg aagtgatcgt gatggcggcg acgaaccgtc cggacgtcct cgatcctgct
     6181 ctcctccgcc cgggccgttt cgatcgccag gtcaccgtcg gcctgccaga ccgcaccggt
     6241 cgcgtcgcga tcctcgcgat ccacacccgc ggcatcccgc tgagttccaa ggtcgatctc
     6301 gagcacatcg ctcgctcgac gacgggcttc tccggcgccg atctcgccaa cctcgtgaat
     6361 gaggccgcgc tcaccgcggc tcgccggaac cgcaaggaga ttctcccggc ggacttcgat
     6421 gaggcgctcg acaagattct gctcggtgtc gccaagagcg gcctgacgaa tccgaaggag
     6481 cgcgaggtcg tggcctacca cgaggccggt cacgccctgg tcgcgcactt cacgcccgga
     6541 tccgatccgc tgcgcaaggt cagcatcgtt ccccgcggta acgcgggcgg cgtcacgatc
     6601 cagatgccgg atgaggaccg cggtctctac agccgcacct atcttctcgg tcgcctcgcc
     6661 ggcatgctcg gtggtcgcgc cgcggagcaa gtcgtcttcg gcgagatcac gacgggtgcc
     6721 gagaatgacc tcaagcaggc cacgagcctt gctcgccgca tggtcggtgt ctggggcatg
     6781 agcgaggacg ttgggccgat ctacctcggt accggcgaag agcacgtctt ccttggtcgt
     6841 gagatcacgc aggacaagtc cttctccgat gcgaccgcgc agcgactcga cgtcgccgtt
     6901 cacggcatcg tcgacaacgc gctgaagcag gcgatcacgt tgaacaacct ccaccgcgac
     6961 aagctcgagg cgctcgtcgc cgcgctgctg gagcgggaga cgctcgacgg accggaggtg
     7021 gtcgcgatct tcggagcccc cccggtcccg gaggatccgg aggcaggcat gacgccgcgg
     7081 cagcccgtaa cgccggacgc cgtcttcggc ggtggctcgg tcgccgacta gcgccaaagc
     7141 caccggcatc ctgatctgtc tgtagtgaaa agccatcggc gttctcgccg gtggcttttc
     7201 catgtcctgc ccgcggacga tatcgattcg ttgacggaag cttcacgttc tcttcgcaat
     7261 tgatggtcgc cgggcttgcg gcaatggcgg atacttggag ccgaagtgac tcacggggac
     7321 ggtgatggat gcctacgtga cgacgaccat cacgacagcg tttgggagcg gcacctgatg
     7381 atcgagcaac gtgtggatga cctgccggat tcgaatctga tcgtggccga gccggcgaac
     7441 ggtgtcgagg aagtcgtcgt tgccgcgccg ggcccggatc tgcccgagct ggttcccgag
     7501 gtctatatca accgggaact gagctggctt gagttcaatc gtcgggtgct gcgcgaagtg
     7561 gccgagaccc cgacgccgct gatcgagcgg gcgaagttcg cggccatctt c
//
LOCUS       DASTMD010000229         1803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3057408, whole genome shotgun sequence.
ACCESSION   DASTMD010000229 DASTMD010000000
VERSION     DASTMD010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3057408"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..>1803)
                     /locus_tag="VFQ54_03445"
     CDS             complement(2..>1803)
                     /locus_tag="VFQ54_03445"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEU0164071.1"
                     /translation="GGWQEVPMHHVDNDRWAGAFDLTENAMWEYAVLAFPDPYSSWID
                     EIEKKLAADLDVSSELLEGRLIIEETLARANLPDTRVIQGALDAAESGGQRDAVNQLT
                     APVVAETILRNRSRLGAARSETLRVSVDRVRARFAAWYSMFPRSAGTVEGQSATFDDV
                     IRQLPRLEGMGFDVLYFTPIHPIATTNRKGRNNSLVAEPGDPGVPYAIGSPDGGHDAI
                     EPSLGTLDDFRRLVAAAADRGMEIALDVALQASPDHPWVAEHPEWFFIRPDGTIKYAE
                     NPPKKYQDIYPLNFNNQNWRALWDEILRVILHWVDNGVRTFRVDNPHTKPTAFWEWLI
                     AEVHRDHPDVIFLSEAFTRPKVMKSLAKAGFAQSYTYFTWRNFKQEIVEYGTELTQTE
                     MKEYFRGNFFTNTHDILPYILQEGGRPAFMTRFALAATMSSVYGIYSGFELCEATPVP
                     GKEEYLDSEKYEFKVWDWDRPGNITDFISRVNAIRREHPALQEYENLRFQQADDDNIL
                     SYSKSSADGEDTVLVVLNLDPFQAHGTTVSLPDELFASDDNAQIQVHDLLSGEPLLWT
                     GRRQYLHLDPHDNPVMFFGLRPWYHQQYAEISY"
BASE COUNT          340 a          548 c          547 g          368 t
ORIGIN      
        1 actagtagct gatctccgcg tactgctggt gataccacgg acgtagcccg aagaacatga
       61 ccgggttgtc atgaggatcc agatggaggt actgccgccg ccccgtccac agcaatggtt
      121 ctcccgagag aaggtcatgt acctgaatct gggcgttgtc gtcactggca aacaactcat
      181 cgggcaacga gaccgttgta ccgtgcgcct ggaacggatc caggttcagg acgaccagga
      241 cggtgtcctc gccatccgcg ctggatttgc tgtagctgag aatattgtca tcatccgcct
      301 gctggaagcg caggttctcg tactcctgga gagccgggtg ctcgcgccgg atcgcgttga
      361 cgcggctgat gaaatcggtg atgttccccg gtcgatccca gtcccagacc ttgaactcgt
      421 atttctccga gtccaggtat tcttccttgc ccggcaccgg tgtcgcctca cagagttcga
      481 agccgctgta gatgccatag acggatgaca tcgtggcggc gagtgcgaac cgcgtcatga
      541 acgcgggacg gccgccctcc tgcaggatat acggcaagat gtcgtgcgtg ttcgtgaaga
      601 agttcccgcg gaagtactcc ttcatctcgg tctgcgtcag ttcggtcccg tattcgacga
      661 tttcctgctt gaaattccgc caggtgaaat acgtgtagct ctgggcgaat ccggccttgg
      721 ccagcgactt cattaccttc ggccgggtga acgcctcgga gagaaagatc acatccgggt
      781 gatcgcgatg cacttcggcg atcagccact cccagaacgc cgtcggtttc gtgtgcggat
      841 tgtcgacgcg gaaggtcctc accccgttgt cgacccagtg aaggatcacg cgcaggatct
      901 cgtcccacag cgccctccag ttctggttgt tgaaattgag cgggtagatg tcctgatact
      961 ttttcggggg attctccgcg tacttgatcg tcccatcggg ccggatgaag aaccactcgg
     1021 gatgctcggc gacccatgga tgatccggcg acgcctgcag cgcgacgtcg agggcgattt
     1081 ccatcccccg gtcggcggca gcggcaacga gacgccggaa atcatcgagt gtgcccagtg
     1141 atggttcgat cgcgtcgtga cctccgtcag gactgccgat cgcataggga acgccgggat
     1201 cgccgggctc ggcgacgagg ctgttgttcc gtcccttgcg attggtcgtc gcgatcggat
     1261 ggatcggcgt gaagtagaga acgtcgaacc ccatcccttc caatcgagga agctgcctga
     1321 tcacatcgtc gaaggttgca ctctgcccct cgaccgtccc ggcggagcgc gggaacatcg
     1381 aataccaggc cgcgaaccgt gccctgaccc ggtcgacgga gacacgaagc gtctccgatc
     1441 tcgccgcacc gagacgggaa cgattgcgaa ggatcgtctc ggcgacgacc ggcgcggtca
     1501 actggttcac cgcgtcacgc tgtcctccgg attccgcggc gtcgagcgcg ccctggatga
     1561 cgcgcgtatc gggcagattg gcccgggcaa gcgtctcctc gatgatgagg cgaccctcca
     1621 gcaattcact cgagacatcg agatcggcgg caagcttctt ctcgatctcg tcgatccatg
     1681 aggaatacgg atcgggaaac gccaggacgg cgtattccca catcgcgttc tcggtaaggt
     1741 cgaatgcccc ggcccaccga tcgttgtcga catggtgcat cggtacttcc tgccagccgc
     1801 cgt
//
LOCUS       DASTMD010000230         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3146874, whole genome shotgun sequence.
ACCESSION   DASTMD010000230 DASTMD010000000
VERSION     DASTMD010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3146874"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VFQ54_03450"
     CDS             <1..764
                     /locus_tag="VFQ54_03450"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164072.1"
                     /translation="YLDRIRSGLAEQGIPFYVLVAPAKWDVYPEKLPDWAQSIRGSGP
                     LDQLLARYPDLPIIDVREPLREAKKDHDVYAAVNPHWTNYGGFVAWDAVATCLRSASP
                     ELGDLGSPAITGVTTEADRNEFSDFGIADPAPDWTVPTYATDLRPVRITDAKGTRTVP
                     GTREIDMLDLPLRTDAQGAATASTALIARDSFGNAFNIPADQEFTTTWQVRHNLDGAA
                     DTQPDLVKLAAQDHPDVLILELTQRYLAIVPAPTD"
     gene            complement(761..1495)
                     /locus_tag="VFQ54_03455"
     CDS             complement(761..1495)
                     /locus_tag="VFQ54_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0164073.1"
                     /translation="MSADAAIRIDDVWKSFRLYRERNQSLKSAIMRGRASIHEDFWAL
                     KGVSFDIRKGETFGLVGDNGSGKSTLLKCLAKILYPNRGSVSMDGRVAALLEVGSGFH
                     PELSGRDNIFLNGSILGMSKREITRKFDEIVDFSGVGEFIDQPVKNYSSGMYVRLGFS
                     VAINVDPDILLVDEVLAVGDESFQRKCAEKFAQFRREGRTVVIVSHALGALSDMCDRL
                     AWLQFGDLRAVGAPDDIIARYRAGEA"
     gene            complement(1492..>2098)
                     /locus_tag="VFQ54_03460"
     CDS             complement(1492..>2098)
                     /locus_tag="VFQ54_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164074.1"
                     /translation="LLPWIFFTNVVNQGMGSLVANESLIKKVYFPRSVLVLSTSASLA
                     VNWAFEMGVLIVAIIIAGAWQVLLWLPGVIVAMILLALFSTGIAMMLSIANAHFRDTQ
                     YFVSIALQLGMYLTPIIYPLSVVVAQSDRIGPVLGSVTLVDIYRLNPLERFTSVFRSL
                     IYDNTWPSVGDTIACLVATIVSLGFGWIIFRRNERSLAEIL"
BASE COUNT          410 a          706 c          651 g          331 t
ORIGIN      
        1 actacctcga ccggatacgc tccgggctcg cggagcaggg cattcccttc tacgtccttg
       61 tagccccggc gaagtgggac gtctaccccg aaaaactgcc ggactgggcg cagagcattc
      121 gcggctcagg tccgctcgac cagctgctgg cccgataccc ggatctaccg attatcgatg
      181 tccgcgagcc gctccgtgag gcgaagaagg atcacgacgt ctatgcggcg gtcaatccgc
      241 actggaccaa ctacggcgga ttcgtcgcct gggacgctgt cgcgacgtgc ctgcggagcg
      301 catctcccga gctcggcgac ctgggcagcc ctgccatcac cggggtcacg accgaggcgg
      361 accggaacga gttctcggac ttcgggatcg ccgacccggc tcccgactgg acggtgccga
      421 cctacgcgac agacctgcgc cccgtccgga tcaccgatgc gaaaggcacg cgcacggtcc
      481 ccggcacccg ggagatcgac atgctcgatc tcccgctccg gaccgatgcg caaggagccg
      541 cgacagcgtc gaccgcgctc atcgcccgcg attcgttcgg caatgccttc aacatcccgg
      601 cggaccaaga gttcaccacg acctggcagg ttcgacacaa cctcgacggc gcagccgaca
      661 cgcaacccga tctggtgaag ctcgccgcgc aggaccaccc ggatgtcctg atcctcgagc
      721 tgacgcagcg ctacctggcg atcgtgccgg cgccgaccga ctaggcctcc cctgcgcgat
      781 accgggcgat gatgtcgtcc ggtgctccca ccgcacgaag gtctccgaac tggagccacg
      841 cgagccgatc gcacatgtcg ctgagcgcgc cgagcgcatg gctcacgatc accaccgtcc
      901 ggccctcccg acggaactgg gcgaacttct cggcgcactt gcgctggaag ctctcgtcgc
      961 ccaccgcgag cacctcatcc acgagcagga tgtcgggatc gacgttgatc gccaccgaga
     1021 agccgaggcg cacgtacatc ccggacgagt agttcttcac aggctggtcg atgaactcgc
     1081 cgaccccgga gaagtcgacg atctcgtcga acttgcgcgt gatctcgcgt ttcgacatgc
     1141 cgaggatcga tccgttgagg aagatgttgt cgcgacctga cagctcggga tggaagccgg
     1201 atccgacctc gaggagggcc gcgacccggc cgtccatcga gaccgatccg cggttgggat
     1261 acaggatctt cgcgaggcac ttcaggagcg tcgacttacc cgaaccgttg tcgccgacca
     1321 gtccgaacgt ctcgcccttc cggatgtcga acgagacgcc cttcagcgcc cagaagtcct
     1381 cgtggatcga ggcgcgcccg cgcatgatcg ccgacttgag cgactgattg cgctcccggt
     1441 acaggcggaa gctcttccag acgtcgtcga tccggatcgc cgcgtctgcg ctcacaggat
     1501 ctccgccaag gagcgctcgt tgcgccggaa gatgatccat ccgaacccga gcgacacgat
     1561 cgtcgcgacg aggcaggcga tcgtgtcacc gacgctcggc cacgtgttgt cgtaaatcag
     1621 acttcggaac accgaggtaa accgctccag cggattgagc cggtagatgt cgacgagcgt
     1681 gaccgatccg agcacgggtc cgatgcggtc ggattgcgcc acgaccacgc tgagcggata
     1741 gatgatcggc gtgaggtaca tgcccagctg cagggcgatg ctcacgaagt actgggtgtc
     1801 gcggaagtgg gcgtttgcga tcgagagcat catcgcgatc cccgtcgaga acagagccag
     1861 gaggatcatc gcgacgatga cgcccggcag ccacagaagc acctgccagg cgcccgcgat
     1921 gatgatcgcg acgatcagga cgcccatctc gaaggcccag ttgaccgcca gagacgcgct
     1981 cgtcgagagg acgaggacgc tccgggggaa atagaccttc ttgatcaagc tctcgtttgc
     2041 gacgagcgac cccatgccct gattcaccac gttggtgaag aagatccagg gcagcaag
//
LOCUS       DASTMD010000231         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_21699, whole genome shotgun sequence.
ACCESSION   DASTMD010000231 DASTMD010000000
VERSION     DASTMD010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21699"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1424)
                     /locus_tag="VFQ54_03465"
     CDS             complement(48..1424)
                     /locus_tag="VFQ54_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU0164075.1"
                     /translation="MSSWSHDAIFYHIYPLGLLDAPLENAIEAPAVSRLPGLASWAPH
                     IRDLGVNALYLGPLFESGTHGYDTIDYRRVDRRLGTNEDLRALVETFHQHGIRVVLDG
                     VLNHVSREFPQFRDLRERGEFSRYRDWFVNVTFGQKSPFGDPFIYDGWSGHLNLVKLN
                     LANPEVREYLFGVVRQWFAEFGIDGLRLDAADVMDKAFLRDLAAVCREANPDCWLIGE
                     IVVGNYAEWAGPGMLDSVTNYEAFKGLYSSLNDRNLFEIAWSLNREFGPEGVYRGLPL
                     YNFADNHDVDRVASLLKNGDDLFPLYAMLFTMPGVPSIYYGSEWGYSGKKQGGDDGPL
                     RPAFAWPVARETMERPELMPWIARLAAIRHETPAIQVGDYLQLHVAAEQFAFLRHMQH
                     ESQSAVVVINAASIPATLSLPVPAIPDGTVLTDAIDISYTVCVKDGAIDLGAVPAATT
                     RILRTAIL"
     gene            complement(1515..2096)
                     /locus_tag="VFQ54_03470"
     CDS             complement(1515..2096)
                     /locus_tag="VFQ54_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0164076.1"
                     /translation="MGDAGRSNREMILAAALDLFADRGYNGVGVEELASSGGVTKPTL
                     YHYFGNKQGLLETLLRERYDPFLKELEAAADYRHDLPNRITKVATLFFDFGTREAKLY
                     RMVIAAGLTARDDVATKLMAQRIEVQDRILRNLFEQASHDHGNMKGRHHLHAAGLQAT
                     ILAYLQLAATGEMTLDDAAIWRLRQQFSHGIYS"
     gene            2368..3054
                     /locus_tag="VFQ54_03475"
     CDS             2368..3054
                     /locus_tag="VFQ54_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU0164077.1"
                     /translation="MRNGRPASAERVAVIGLGRFGSSLAGTLNELGYEVTAIDLDARR
                     IQEIANLVTLAAQGDGTDEALLRQLDVEHSDVAVVAQGENLEASVLTTLLLKKLEIPW
                     VVAKAKTSLHGELLRKVGADRVIFPEVDAGKRLAHSLGVRHISDYISLSPAVGVAKLE
                     VPAYLAGKSLANCKIHLESMIDVLLIQRGADLIVAPGDQEILQPHDEIVVVGADVDIE
                     SFVAPPGAPA"
     gene            3054..4478
                     /locus_tag="VFQ54_03480"
     CDS             3054..4478
                     /locus_tag="VFQ54_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEU0164078.1"
                     /translation="MAETRDRGNGTVRAGRRIIHLAEIPRRPGAPTVQMHAQRFVLAF
                     VAIVLIGWGLLALPVAAEAGQTTSPIDALFTSLSAASVTGLVVVDTQQHWSFFGEFVI
                     FVLMQIGGLGFMVGASLVLVALGRGSSLRDALMLQDGAPTLSVREAVDLSKRVMRFIL
                     VCEGIGAVLLTIRFWRDQPPLDAIWFGLFHAVSAFCNAGFDLEGGYRSMAGYETSPWM
                     NVVLIVLIQAGALSYMVLSETWSVRRWSKFSLDTKLVLLTNAILIVLGAMLFLVGEWN
                     GVLAKTGMLAKPMVAIFQSTSMRTSGITTVPFGDAHATTDFLSVGLMMIGGASGSTAG
                     GVKLATTAVIVIAVVSTLRGQSQTQAFGRRVPTSQVFRAMAIIVVYLAVHFAFTLALA
                     FTEDVLGGKNLPFLSLMLETMSATATAGLTSGIVPSLSDAGKLVLCVAMFFGRLGPLT
                     LVYALTRREQVTRYRYPESPIRMG"
     gene            4582..>5199
                     /locus_tag="VFQ54_03485"
     CDS             4582..>5199
                     /locus_tag="VFQ54_03485"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU0164079.1"
                     /translation="MKDLMRAIFYEQFGVTPRLVDLPKPEPSANGVVVQVEATGLCRS
                     DIFGWRGKDRDIVVPHVPGHELVGTIANVGAEVRNFAVGQRVTVPFVCACGNCDECAS
                     GNAQVCRNQTQPGFTHWGSYAEYVAIHDADINLIALPDGMDSGAAALLGCRFATAFRG
                     VVHQAKLTSGQLLLVVGCGGVGLSAVMIGLAVGARVIAVDISRNAL"
BASE COUNT          870 a         1660 c         1698 g          971 t
ORIGIN      
        1 gggttggtag ggcggggttt cataccccgc cgcaaacggg cgcgccccta caggatcgcc
       61 gttcgcagga tgcgcgtcgt cgcggcggga acggcgccga ggtcgatcgc gccatccttc
      121 acgcagaccg tgtaggagat atcgatcgcg tcggtcagaa cggtgccgtc cggaatcgcc
      181 gggaccggca acgacagcgt cgccggtatg gatgccgcat tgatcacgac caccgccgat
      241 tgcgattcgt gctgcatgtg gcgcaggaag gcgaattgtt ccgctgcaac gtgcagttgc
      301 aggtagtcgc ccacctggat cgccggtgtc tcgtggcgga tcgcggcgag ccgtgcgatc
      361 catggcatca actcgggacg ttccatcgtc tcgcgggcaa ccggccaggc gaaagcgggt
      421 cgcaggggac cgtcatcgcc gccctgcttc ttgccgctgt atccccactc cgacccgtag
      481 tagatggacg ggacgcccgg catcgtgaag agcatcgcat agagtgggaa gagatcgtcg
      541 ccgttcttca ggaggctggc gacacgatcg acatcgtggt tgtccgcgaa gttgtagagc
      601 ggcagtcccc ggtagacgcc ttccggtccg aactcgcggt tcagcgacca ggcgatctcg
      661 aagagattgc gatcgttgag gctcgagtag agccccttga acgcctcgta gttcgtgacc
      721 gaatcgagca tcccgggacc agcccactcg gcatagttgc cgaccacgat ctcgccgatc
      781 agccagcagt ccggattcgc ctcgcgacag acagcggcga ggtcgcgcag gaatgccttg
      841 tccatcacgt ccgccgcatc gagccgcagg ccgtcgatgc cgaactccgc gaaccactgc
      901 cggacgacgc caaagagata ctcccggacc tccggattcg cgaggttgag tttcaccaga
      961 ttgaggtgac cgctccaccc gtcgtagatg aagggatcgc cgaaggggct cttctggccg
     1021 aaggtcacgt tcacgaacca gtcgcggtag cgcgagaatt ccccgcgctc gcgaagatcg
     1081 cggaactgcg ggaactcgcg gctgacgtgg ttcagcacgc cgtcgagaac gacacgaatc
     1141 ccatgctggt gaaaggtctc caccaacgcg cgcagatcct cgttcgtccc gaggcggcga
     1201 tcgacgcgcc ggtagtcgat cgtgtcatag ccgtgggtgc cggattcgaa gagtgggccg
     1261 aggtagagcg cgttgacccc gagatcgcga atgtggggag cccagctggc cagtcccgga
     1321 agacgggaga cggcgggcgc ctcgatggca ttctcgagcg gggcgtcgag cagcccgagc
     1381 ggatagatgt gatagaaaat ggcgtcgtgg ctccatgaac tcatgggcgt tctcctctgg
     1441 atcgggcgcg atcgaccggt atggtgtcgg ggcaaaccgg gatcgagtgg ctggcggtcg
     1501 ctcgcgggac ggactcagct atagattccg tgcgagaact gctggcgcag tcgccagatc
     1561 gccgcgtcat cgagcgtcat ttccccggtc gcggccagtt gcaggtaggc gagaatcgtc
     1621 gcctgcagac cggcggcatg cagatgatgg cgccccttca tgtttccgtg gtcgtgactc
     1681 gcctgttcga agaggttgcg caggattcga tcctggacct cgattcgttg ggccatcagt
     1741 ttcgtggcga catcgtcgcg ggcggtgagg ccggcggcga tcaccatccg gtagagcttg
     1801 gcttcccgcg tcccgaaatc gaagaagagc gtcgccacct tcgtgatccg gttcggcaag
     1861 tcgtgccggt agtcggcggc cgcttcgagt tccttgagga agggatcgta tcgttcccgg
     1921 aggagggttt cgagcagccc ctgcttgttt ccgaagtagt ggtaaagcgt cggcttcgtg
     1981 acaccgccag agcttgcgag ttcttcgacc ccgaccccgt tgtatccacg atcggcgaag
     2041 agatccagcg ctgcggcgag gatcatctcc cggttcgaac gtcccgcgtc acccaccttg
     2101 tcctgcttcc attctggctg tataccgatc ggtacccagc tggcatcact ataccgatcg
     2161 gtatacgaca tgtcaagcgc gtcgtggagt ctcccgcatc aattcccgcg aagtgtgcgc
     2221 gaaatcgttc cacgcgcaag cccgacatcc atcgcgacga tcctcgcggt caacgcccgc
     2281 cgcttctggc cgcgtagaat gaatgcaacg aagaaaggca cgccacggca catggagcga
     2341 cgtcgatttc ccggattccc cggcttgatg agaaatggca gaccggcctc cgccgaacgc
     2401 gtggcggtca ttggtcttgg acgattcggc agcagcctcg ccgggacgct caacgaactt
     2461 ggctacgaag tcaccgccat cgacctcgac gcccggcgga ttcaggagat cgcgaacctg
     2521 gtgacgcttg ccgcgcaagg ggatggtacc gatgaggcgc tgctgcgcca gctcgatgtc
     2581 gagcactcgg atgtcgccgt cgtcgcccag ggcgagaacc tcgaggcaag cgtcctcacc
     2641 acgctcctgc tcaagaagct ggagattccg tgggtcgtgg ccaaggcgaa gacgtcgctc
     2701 cacggcgagc ttctccgcaa ggttggcgcc gatcgcgtga tcttcccgga ggtcgatgcc
     2761 gggaagcggc tcgcccactc gctcggtgtc cgccacatca gcgactacat ctcgctctcg
     2821 ccggcggtcg gcgtcgcgaa actcgaggtg cccgcctacc tggccggcaa gagcctcgcc
     2881 aactgcaaga ttcacctcga atcgatgatc gacgtgctcc tgatccagcg cggcgcggat
     2941 ctcatcgtgg cgccgggtga ccaggagatc ctccagccgc atgacgaaat cgtcgtcgtc
     3001 ggcgccgatg tcgacatcga gtccttcgtt gcgccccccg gggcgcccgc ctgatggccg
     3061 aaacgcgcga tcgcgggaac ggaacggtgc gcgccgggcg gcgcatcatt cacctggccg
     3121 agattccacg gcgacctggc gcgccgacgg tgcagatgca cgcgcagcgc ttcgtgctcg
     3181 ccttcgtcgc catcgtcctc atcggttggg gattgctcgc gcttcccgtc gccgcggagg
     3241 cgggacagac gacgagcccg atcgacgccc tgttcacctc gctctccgcg gcgtcggtca
     3301 ccgggttggt cgtcgtcgat acgcagcagc actggagctt cttcggtgag ttcgtgatct
     3361 tcgtcctgat gcagatcggc ggcctgggat tcatggttgg cgcatcgctc gtcctcgtgg
     3421 cgttgggacg aggcagcagc ctgcgcgacg cgttgatgct gcaggatggc gcgccgacgc
     3481 tctccgtccg ggaggcggtc gatctctcga agcgagtcat gcgcttcatc ctcgtctgcg
     3541 aggggatcgg cgcggtgctg ctgacgatcc gcttctggcg ggatcagcca ccgctcgatg
     3601 cgatctggtt cgggctcttc catgccgtct ccgccttctg caatgccggc ttcgatctcg
     3661 agggcggcta tcgatcgatg gcggggtatg agacgtcgcc ctggatgaac gtggtgctga
     3721 tcgtgctgat ccaggcgggg gcgctctcct atatggtact cagcgaaacg tggagcgtcc
     3781 ggcgctggag caagttcagc ctcgatacga agctcgtcct gctgacgaac gcgatcctga
     3841 tcgtgctggg agcgatgctc ttcctcgtcg gcgagtggaa cggcgtgctc gcgaagacgg
     3901 gaatgctcgc gaagccgatg gtggcgatct tccagagcac gtcgatgcgc acctccggca
     3961 tcacgacggt tcccttcggc gacgcccatg caacgacgga tttcctctcc gtcggattga
     4021 tgatgatcgg tggcgcttcg ggctcgactg cgggcggcgt caagctcgcg acgacagcgg
     4081 tcatcgtgat cgccgtcgtc tccacgcttc gcgggcagtc gcagacgcag gcattcgggc
     4141 gacgggtccc gaccagccag gtcttccggg cgatggcgat catcgtcgtc tatctcgccg
     4201 tgcactttgc cttcacgctt gcgctcgcct tcaccgagga cgtgctcggc ggcaagaacc
     4261 tgccgttcct ctcgctaatg ctcgaaacga tgagcgcgac ggcgacggcg ggactgacct
     4321 cgggcatcgt cccgtcgctt tcggacgcgg gaaagctcgt gctctgcgtc gcgatgttct
     4381 tcgggagact cgggccgctg acactcgtct acgccctgac ccgtcgcgag caggtgaccc
     4441 gctatcgcta tcccgagtcg ccgattcgca tggggtagct ctcgccgcgt cggcgttcgg
     4501 ggtatccgcc gttttcaggg aacgcgagat cgcgcacaat ggtctgcgtg caggccgaat
     4561 ctcctcgttt ccgtgaacca gatgaaagac ctcatgcgcg cgatcttcta cgaacagttt
     4621 ggcgttacgc cgcgcctcgt cgatcttccg aagccggagc cgagcgcgaa cggcgtcgtc
     4681 gtccaggtcg aggcgacggg tctctgccgg agcgacatct tcggctggcg ggggaaagat
     4741 cgcgacatcg tcgttcctca tgtcccggga catgaactcg tcgggacgat cgcgaacgtg
     4801 ggggcggaag tccggaactt cgcggtcgga caacgggtga cggtcccctt cgtctgcgcc
     4861 tgcgggaact gcgacgaatg cgcctccggc aatgcccagg tctgccgcaa ccagacgcaa
     4921 cccggcttca cccactgggg atcctacgcc gaatatgtcg cgatccacga tgccgacatc
     4981 aacctgattg ccctcccgga cggtatggat tccggcgccg ccgcgctgct cggctgccgc
     5041 ttcgccacgg cgttccgcgg ggtcgtgcac caggcgaagc tcacctccgg ccagctgctc
     5101 ctcgtcgtcg gctgcggcgg cgtcgggctc agcgcggtga tgatcggcct ggccgtggga
     5161 gcccgcgtca tcgccgtcga catcagccgg aacgcgctc
//
LOCUS       DASTMD010000232         1682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1182604, whole genome shotgun sequence.
ACCESSION   DASTMD010000232 DASTMD010000000
VERSION     DASTMD010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182604"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFQ54_03490"
     CDS             <1..600
                     /locus_tag="VFQ54_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164080.1"
                     /translation="GTMTARWGYRTAGLEVPADTHSAGISLNAGVSTFGSQGITISNE
                     TNTDLTVLQLIVTSGGETYPESSLNAAPSVSIQTLATATIDVPAGHLVVSATTIRLGQ
                     ANTSGGNTGDIGTIGVVLDGSIVMQPKKGEVGTRPDATTALTPQPNGKSVVVDTGGQY
                     VGGPNGEIAIGGASAGRAYVAEITTSTIPDNVATPVSGP"
     gene            complement(800..>1682)
                     /locus_tag="VFQ54_03495"
     CDS             complement(800..>1682)
                     /locus_tag="VFQ54_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0164081.1"
                     /translation="GWEASIVNLLSPGDRIVVTVTGDFGDRYAKTAAKLGLEVFRLDV
                     EWGTAVTAEMLARAFDEHGPFKAAFITHNETSTGLTNPLPELAKVARENDALVVVDAV
                     SSAAAIPLKVDEWDLDWVLSGSQKAWMCPPGLMISAVSDRAMAASEKTGFPRFFWDVQ
                     TMAAATAARTTPVTPALSLLYGLDAALIAMLDEGMEQIWARHARLGDVVRKGLTEIGL
                     ELLADPAYASDSITAFRPPAGVTSTEFHDRLRATSGIEIAIGQGHLAQSLLRLGHMGY
                     VEQPELDATLEAIAKIV"
BASE COUNT          310 a          584 c          514 g          274 t
ORIGIN      
        1 ggaacgatga ctgcccgatg gggatatcga acggcagggc tcgaagtccc ggcggacacg
       61 cactcggcag gaatctcgct gaacgcggga gtttccacct ttggcagcca gggcatcacg
      121 atctccaacg agacaaatac cgatctcacg gtcctgcaac tcatcgtgac atcaggcggt
      181 gagacctatc ccgaatcgag cctcaacgcc gctccctcgg tttcgatcca gacacttgcg
      241 accgccacga tcgatgttcc tgcgggccac ctcgtcgtat cggcaacaac gatccggctc
      301 ggtcaggcca acacgtccgg cggcaataca ggagatatcg gcacaattgg cgtcgttctg
      361 gatggctcga tcgtcatgca gccaaagaaa ggtgaggtcg gcacccgccc cgacgccacc
      421 actgcgctga cacctcaacc aaacgggaag tccgtcgtgg tcgataccgg cggtcaatac
      481 gttggtggtc cgaacggcga gatcgccatc ggtggcgcgt ccgctgggcg tgcgtatgtg
      541 gcagaaataa cgaccagtac catccccgac aacgttgcga cacctgtgtc cggcccatga
      601 cttgccgcaa gctcacggga tatgttgcgc aagtattgac aacagaagat ggcgggctct
      661 tcgcgagccc gccatcttct gttcgactgc attcggcgca gatccccggg gccccgccgc
      721 cggcagagga tccggtcgtt cgtagggcgg gaccgccgct gccgggccgc agtccggacg
      781 gcctcttccg cctcccggtc tagacgatct tcgcgatcgc ctccagcgtc gcgtcgagct
      841 ccggctgctc cacgtatccc atgtgaccga ggcgaagcag tgactgggca agatgcccct
      901 gcccgatcgc aatctcgatc ccggacgtcg cccgcagcct gtcgtggaac tcggtcgagg
      961 tcaccccggc cggaggccgg aacgccgtga tcgagtcgga cgcgtacgcc ggatcggcca
     1021 gcagctcgag accgatctcc gtcagtccct tgcggaccac gtcacccagt cgcgcgtggc
     1081 gcgcccagat ctgctccatc ccctcgtcca gcatcgcgat cagcgccgcg tcgagcccgt
     1141 agaggagact cagcgccggc gtcacgggcg tggtgcgggc agcggtcgcg gcagccatcg
     1201 tctggacatc ccagaagaag cgggggaacc ccgtcttctc cgacgccgcc atcgcccgat
     1261 cactcaccgc cgagatcatc agaccgggag ggcacatcca cgccttctgc gagccggaca
     1321 gcacccagtc gagatcccac tcgtcgacct tgagcggaat cgcggcggcg gaactcaccg
     1381 cgtcgacgac gaccagggcg tcgttctcgc gagcgacctt cgccagttcc ggcagcggat
     1441 tggtgagccc ggtggacgtc tcgttgtgcg tgatgaacgc cgccttgaaa ggaccatgct
     1501 cgtcgaacgc ccgcgccagc atctccgccg tcaccgcggt gccccactcg acgtcgaggc
     1561 gaaagacctc gagtccgagc ttggccgcgg tcttcgcgta gcgatcgccg aagtctcccg
     1621 tcacggtgac cacgatccgg tcgccgggcg agaggagatt gacgatcgac gcctcccagc
     1681 cc
//
LOCUS       DASTMD010000233         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1539840, whole genome shotgun sequence.
ACCESSION   DASTMD010000233 DASTMD010000000
VERSION     DASTMD010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539840"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFQ54_03500"
     CDS             complement(<1..276)
                     /locus_tag="VFQ54_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164082.1"
                     /translation="MLVQDILNEETAVDVTTSFQKWQRDTLLSGLLPVIGVAGTSGKS
                     TVVRLLDRIFRDAGLRTATWTNLGIEIRGRRQRGELSGWALALSRLAE"
     gene            complement(276..971)
                     /gene="rdgB"
                     /locus_tag="VFQ54_03505"
     CDS             complement(276..971)
                     /gene="rdgB"
                     /locus_tag="VFQ54_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEU0164083.1"
                     /translation="MVVLWKAANRMTTTSGEEVIDSKRLSVVLATENQGKVRELQALL
                     GPDVQVQDLTAAGISLPEEVGATFAENALAKAIFASERTGLPALADDSGLEVQALDGL
                     PGVRTARYAGEDATDADNRQKLLGVLKDVAVEDRGARFVSVIAVVVPGEEPQTFDGYC
                     QGTIGFAERGTGGFGYDSIFVLADGRTMAELTPEAKNAISHRGAAMRQAVPALLERLK
                     ATTAAGDSRSEVG"
     gene            complement(938..1765)
                     /locus_tag="VFQ54_03510"
     CDS             complement(938..1765)
                     /locus_tag="VFQ54_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU0164084.1"
                     /translation="MTELAGTTIELDVQPPAGLLIDTHCHLDDQSFREDLSEVLAESM
                     ASGVRSWINVGFEPERWDSTLRLVDRFPGMSCMLGIHPTSATAYSGETLDRMRRLLLA
                     SDAVAIGEIGLDLLRERETESIQRQAFEAQLDLALELRLPVVIHMRDAEAEMLSVLAA
                     RPTLPPLLFHSFDGGPALTEFILASGAYVGVGGLATRQKSESLRAELQKIPLQRMVLE
                     TDSPYLVPARQKARRNTPAQIRTIASFLATLLAIDEDQVVHTTTINAWSYFGRLPIA"
     gene            complement(1769..>2310)
                     /locus_tag="VFQ54_03515"
     CDS             complement(1769..>2310)
                     /locus_tag="VFQ54_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU0164085.1"
                     /translation="DDGGAASRAVQATIAALREMEGDTELAARIVRIASRVATSEHVF
                     TIGRGTGFASAQEAALKIKEGSYVHAEAFAAGELKHGAIALIEPGTPCLMFASSSGAD
                     AQLVNTSQELLSRGAYTIGVGMTAPEGFSDVIPFPHDTFVAEIVQVYVAQRIAYQTAI
                     LRGLDPDRPRNLAKSVTVR"
BASE COUNT          403 a          736 c          757 g          414 t
ORIGIN      
        1 ttcggccagg cgggaaagcg cgagcgccca gccgctcagt tccccccgct ggcgccgtcc
       61 tcgaatctcg ataccgagat tggtccaggt ggcggtgcgc agaccggcgt cccggaagat
      121 gcggtcgagc agacggacga ccgtactctt gccactcgtg ccggccacgc cgatgaccgg
      181 aagaaggccg gaaaggagcg tgtcgcgttg ccacttctgg aagctggtcg tgacatcaac
      241 agccgtctct tcgttgagga tgtcctgaac gagcatcacc cgacctcgct tcgagaatcc
      301 ccggccgcgg tggttgcctt cagccgttcc aacaaggcgg gaacggcctg tcgcattgcg
      361 gcgccgcgat ggctgatcgc gttcttcgcc tcgggtgtga gttccgccat ggtgcgaccg
      421 tcggcgagga caaagatcga gtcgtagccg aagccaccgg tgccgcgttc cgcgaaacca
      481 atcgtgccct ggcaatagcc gtcgaaggtc tgaggctcct cgccgggcac cacgaccgcg
      541 atgacgctca cgaaccgggc gccgcgatcc tccacggcga catccttcag aacgccgagc
      601 agtttctgac gattgtcggc atcggttgca tcctcgccgg cataacgcgc cgtgcgaacc
      661 ccaggaaggc cgtcgagcgc ctgcacttcg agaccggagt cgtcggcgag tgcgggcagc
      721 cccgttcgct ccgacgcgaa gatcgccttt gccagggcat tctcggcgaa cgtcgcgccg
      781 acttcctccg ggagggagat gccggcggcc gtcaggtcct ggacctgcac gtccggtccc
      841 agcagcgcct ggagctcgcg aaccttgcct tgattctccg tcgcgagaac gacggagagt
      901 cgtttcgagt cgatcacttc ttcacctgac gtcgtcgtca tgcgattggc agccttccaa
      961 agtacgacca tgcgttgatt gtcgttgtgt gaacgacctg gtcttcatcg attgcaagaa
     1021 gcgtcgcgag aaagctcgcg atcgtccgga tctgggcggg ggtgttacga cgggccttct
     1081 ggcgcgccgg gacgagatac ggggaatccg tctccaggac catacgctgg agcgggattt
     1141 tctgaagttc ggcgcgcagt gattccgact tctgcctcgt cgccaggcca cccacaccaa
     1201 cgtacgcccc actggcgagg atgaactcgg tcaatgcggg gccgccgtcg aacgaatgga
     1261 agagcagggg cggcagcgtg gggcgggcgg caaggaccga gagcatctcg gcctcggcgt
     1321 cgcgcatgtg gatcacgacc ggcaaccgga gttcgagggc gaggtcaagc tgggcttcga
     1381 acgcctgacg ctggatcgat tcggtctcgc gctcccggag gaggtcgagg ccgatctcgc
     1441 cgatggcaac cgcgtcggac gcgaggagca ggcgccgcat gcgatccagt gtctccccgg
     1501 aatacgccgt ggcgctcgtc gggtggatgc cgagcatgca ggacatcccc ggaaaccggt
     1561 cgacgagccg gagggtagag tcccaccgct ccggctcgaa gccgacattg atccagctgc
     1621 gaacgccgct cgccatgctc tccgcgagta cttcggaaag atcctcgcga aacgactggt
     1681 catcgaggtg gcaatgggtg tcgatcagaa ggccggcggg cggctgaacg tcgagttcga
     1741 ttgtggtgcc ggccaattcc gtcattgcct atctcacggt cacgctcttc gccagattgc
     1801 gtggtcggtc cgggtcgaga cctcgcagga tcgctgtctg gtaggcgatc cgctgcgcga
     1861 cataaacctg aacgatttcg gccacgaacg tgtcgtgggg aaaggggatc acgtcgctga
     1921 agccctccgg ggcagtcatg cctactccga ttgtatatgc tccacgactg aggagttcct
     1981 gagatgtatt gacgagttgg gcgtcagcac cagatgaact ggcgaacatt aggcagggcg
     2041 tgcccggctc gatgagcgcg attgccccgt gcttcaactc gccggcggca aacgcctcgg
     2101 cgtggacgta gctgccttcc ttgatcttca acgctgcctc ctgcgcgctc gcgaatcccg
     2161 tcccgcgccc gatcgtgaag acgtgttccg acgtcgcgac ccgggaggcg atgcgcacga
     2221 tccgggccgc gagttcggtg tcgccctcca tctcccgcaa ggcggcgatc gtggcctgaa
     2281 ccgcgcgcga ggctgcgcca ccgtcgtctc
//
LOCUS       DASTMD010000234         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2611730, whole genome shotgun sequence.
ACCESSION   DASTMD010000234 DASTMD010000000
VERSION     DASTMD010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2611730"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /gene="nrdJ"
                     /locus_tag="VFQ54_03520"
     CDS             <1..1440
                     /gene="nrdJ"
                     /locus_tag="VFQ54_03520"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-triphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HEU0164086.1"
                     /translation="WAKAVGKILAMKKPAKRIVLDFSEVRAAGIRLRGYGWISSGDGP
                     ISAALEQICRLLSAKASKLLTRIDLLDVMNLLGTTLSSRRSAEIALVPYGDPEWEEFA
                     LAKRDYWATGKEHRAQSNNSLLFWTKPSKAELYGIFYLMMMAGGSEPGFINAAHARRR
                     GPWFEGCNPCVEILLGDKSFCNLTETDLAKFNGDWEGLLKAHRLVARANYRQTCVNLK
                     DGVLQHSWHELNEYLRLCGVGVTGIVRWEYADEALRWRTLKAVARDGAMSMADELGLP
                     QPKAVTTVKPSGTLSKIMDTTEGVHRPLGRYIFNNVNFSKHDPLVDTLQDAGYRVIQN
                     PIDPTGVVVTFPVSYDDVAFDVTDDGREVNLEPAVSQLDRYKFLMDNYVDHNCSVTVS
                     YSPDEVPAIVNWLDENWDSYIGVSFLYRTDPTKTAADLGYPYLPQEVVTKEVFDKYAL
                     TLKAIDLDGVEAFDALIDDACATGACPVR"
     gene            1453..1725
                     /locus_tag="VFQ54_03525"
     CDS             1453..1725
                     /locus_tag="VFQ54_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164087.1"
                     /translation="MRCKFKVYHVVEQDRSVYDPLTHAMDKREATFSVFLSPVMNDGT
                     PENAKFFEATPAGQIELHGITPTAAAGMEEGAEFYVDFTLTAGKDA"
     gene            complement(1976..>2637)
                     /locus_tag="VFQ54_03530"
     CDS             complement(1976..>2637)
                     /locus_tag="VFQ54_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU0164088.1"
                     /translation="SNVILPQPRNDNQRLYLKALADDPQVVGLGPAGTGKTFLAASMA
                     AFLYLRSRVDRIVVTRPAVDCGEQHGFLPGKLEAKLAPWVRPVFEIIEEQLGKAKVID
                     MIRDGDLELAPLGMLRGRTLDNAFVILDEAQNATPRQMEMFVTRIGEGTRVVLNGDLG
                     QSDLSGESGLGVAVDLIRNHGVPAAIVEFTEADVVRSGLCKAWVDGYRSRREQGRNIH
                     A"
BASE COUNT          512 a          831 c          811 g          483 t
ORIGIN      
        1 tgggcgaagg cggtcggcaa gatcctcgcc atgaagaagc ccgccaagcg gatcgtgctg
       61 gacttcagcg aggtccgggc tgccggcatt cgccttcgtg gctatggatg gatcagctca
      121 ggcgacgggc cgatcagcgc tgcgctcgaa caaatctgcc ggctgctctc ggccaaggcc
      181 agcaagctgc tgacgcggat cgatctgctc gacgtcatga acctgctggg cacgaccctg
      241 tcgtcccgcc ggtcagccga gatcgccctg gttccctacg gcgatcctga gtgggaggag
      301 ttcgcactcg ccaagcggga ctattgggcg actggcaagg agcaccgagc tcagtcgaac
      361 aactccctgc tgttctggac gaagccctcg aaggcggaac tctacggcat cttctatctg
      421 atgatgatgg ccggtggctc cgagccgggc ttcatcaatg cggcccacgc ccgccggcgc
      481 ggcccgtggt tcgagggatg caacccgtgc gtcgagatcc tgctcggaga taaatccttc
      541 tgtaacctga ccgagaccga cctggccaag ttcaatgggg attgggaggg gctgctgaag
      601 gcgcaccgtc tggtcgcccg ggcgaactac cggcagactt gcgtcaacct caaggacggc
      661 gtgctgcagc actcctggca cgagctcaac gagtacctcc ggctctgcgg cgtcggcgtc
      721 accggcatcg tccgctggga atatgccgac gaagcgctcc gttggcggac gttgaaggcc
      781 gtggctcggg acggtgcgat gagcatggcg gacgagctgg ggctgccgca gccgaaggct
      841 gtgaccacgg tcaagcccag cggcacgctg tcgaagatca tggacacgac cgagggcgtc
      901 caccggccgc tcggtcgata catcttcaac aacgtcaact tctcgaagca cgacccgctg
      961 gtcgatacgc ttcaggacgc gggctatcgg gtcatccaga acccgatcga ccccaccggc
     1021 gtcgtcgtta ccttcccggt gtcctatgac gatgtcgcct tcgacgtcac cgacgacggc
     1081 agggaggtga acctggagcc ggccgtctcc cagctcgatc gatacaagtt cctgatggac
     1141 aactacgtcg atcacaactg ctcggtgacg gtgtcctatt cgcccgacga ggtgccggcg
     1201 atcgtcaatt ggctcgacga gaattgggac agctatatcg gcgtctcgtt cctgtatcgg
     1261 accgacccga cgaagaccgc tgcggatctc ggctacccgt acctgccgca ggaggtcgtc
     1321 accaaagagg tgttcgacaa gtacgccttg acactgaaag cgatcgatct cgacggcgtc
     1381 gaagcctttg acgccctgat tgatgacgcg tgcgcgaccg gcgcctgtcc ggttcgatag
     1441 gagagaaaga acatgcgctg caaattcaag gtctaccacg tcgtcgagca ggaccgatcg
     1501 gtatacgatc cgctgaccca cgcgatggat aagcgggagg ctaccttctc ggtcttcctg
     1561 tcaccggtga tgaacgatgg caccccggag aacgccaagt tcttcgaggc cacccccgcc
     1621 ggccagatcg agctgcacgg catcactccc acggcggctg cagggatgga ggaaggcgcc
     1681 gagttctacg tcgacttcac ccttacggcc gggaaggatg cgtaaccgat cgcgctgtct
     1741 ccggtccagc ttggcatagt agtgtgggca cctcggtgcc cacacttcat acatttggcc
     1801 tttcgcctca atagcggcag cggatcagat acctgccaca gaagcacgaa aggaacgagg
     1861 gctacggctc gatagtgcga gcacgatgga cacgtcaacc agcaccatcg cggctcgctt
     1921 aggggctccg gaacccacaa ggtcgccttc gtaaacatgg gtggaggcta ccatatcagg
     1981 catggatgtt cctaccttgt tctcgtcggg accgataacc atctacccat gccttgcaga
     2041 gaccggaccg gacgacatcg gcttcggtaa actcgacgat cgccgccggc acgccgtggt
     2101 tcctgatgag atcgacggcc acccccaggc cagactctcc cgaaagatcc gactgaccta
     2161 gatcaccgtt cagcacgacg cgtgtgcctt cgccgatccg ggtgacgaac atctccatct
     2221 gcctgggtgt ggcgttctgg gcctcgtcta ggatcacgaa ggcattgtcg agcgtccgtc
     2281 cccgcaacat ccccagcggt gccagctcga gatcgccgtc gcgtatcatg tcgatgacct
     2341 tggcctttcc cagctgctcc tcgatgatct caaacaccgg gcgtacccat ggtgcgagct
     2401 tggcctccag cttgcccggc aggaaaccgt gctgctcgcc gcagtccacg gccggccggg
     2461 tgacgacgat ccggtcaacc ctgcttcgca gatacaagaa ggccgccata gaggcggcca
     2521 ggaaggtctt accggtacct gccggtccca gtccgacgac ctgggggtcg tcggctaggg
     2581 ccttgaggta gagtcgctgg ttgtcgtttc gaggctgagg aaggatgacg ttggagc
//
LOCUS       DASTMD010000235         4477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_914021, whole genome shotgun sequence.
ACCESSION   DASTMD010000235 DASTMD010000000
VERSION     DASTMD010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914021"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFQ54_03535"
     CDS             complement(<1..284)
                     /locus_tag="VFQ54_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU0164089.1"
                     /translation="MTDTRTRTFNEVSTKVDFPAMERDLIEWWKAHEILEQYLHRNDG
                     SSERFSFIDGPITANNPMGVHHGWGRTYKDVFQRYNAMLGKKQRFQNGFD"
     gene            complement(692..1423)
                     /locus_tag="VFQ54_03540"
     CDS             complement(692..1423)
                     /locus_tag="VFQ54_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU0164090.1"
                     /translation="MALSSDSKTTSRWFLRDRLDPDQLAFTRGKRGISIATVLLGIFA
                     CLFFIVRTKRSSKLDARITTRLQAKDHPLWDRLMHLVSWPGFPPQSRLLPPSLAAILW
                     ARGFRLEAVFQLMAWGTGGISFTVKRIMRRSRPGPDDAGIRVIKANIGGSSFPSGHVI
                     NYVGVYGFLTFLAFSWIKPKKWRRAIVGGLGSLIALVGPSRIYLGHHWLTDVLASYTL
                     GGTYLILLTNLYRKVRLALGRHAQG"
     gene            1578..2384
                     /locus_tag="VFQ54_03545"
     CDS             1578..2384
                     /locus_tag="VFQ54_03545"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEU0164091.1"
                     /translation="MSRPVIQTIRTYWLWCAIVAAAVVVFLASPGATGNKAHSVLHGL
                     CAQTPSHTFTFGGQPLPFDGRMTGIYGGSILTFAWLWIRGRVLFYGNPPKRVIAVLTL
                     FVIALAVDGFNSLFHDLGIWYPYEPQNIYRLITGFGTGMSLSIILSWLLASSMWNLSR
                     PTTGIRSLRDVGAIALLAIPYGAAVLSGWSWLYLPLTALLLGSAWLTLSILMLVVVLL
                     LFRIDEKVRAVPQLHLPGAAASILGLVIMLTLAGGRFWLEHTFGITVDIS"
     gene            2543..3292
                     /locus_tag="VFQ54_03550"
     CDS             2543..3292
                     /locus_tag="VFQ54_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164092.1"
                     /translation="MRIIRHTTYIQQRKRRARGIAFVGFAVLAASLWIALNPSLLIPA
                     YGAMFVGFITFNIGMQQVGKWSRNPRNDQMLDRQLERLGERYTLIHFPPVGKHKAEHV
                     LVYPGGLLALTAREIDGKVETTGNKWRKRSAGLRRIFGFSGPQLGNPSSDTDAALGEL
                     ETFLEEHQLELDVEGIIVFVHPLVELEVEEPEYPVLHGEEVPSFVQSLPADESFSTAE
                     RDAVVALLGAGEVVEAPTRQTRRRPVKRRAA"
     gene            3308..4303
                     /locus_tag="VFQ54_03555"
     CDS             3308..4303
                     /locus_tag="VFQ54_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HEU0164093.1"
                     /translation="MERSQSRENWGVWGHGRTVRDLQRAAASSPRHSYIISGPASAGK
                     KTIALAFAKALSCASPPAPGLFCGECSACRRIDRGTFPDVTFVDLEMQSSREKSTSRN
                     TSLNIATVREISANVMLRPLESRWRIAIVDDVETMQETAQEAFLKTLEEPPAYTILLL
                     LTSDADLLLPTIRSRCLTLSLQAVPVKAIADGLESIGTASDEAGVIAEASAGLPGWAI
                     RAAADPALLESQVAAAGEAHAWARSGEYERLVTATRIADQFLKDREAVFSRLLTVQRA
                     WRGLLHDAVNGDASVSAESSVTALKSIETCLLDLESNVRPRLALQQMVLQWPVIR"
BASE COUNT          782 a         1427 c         1402 g          866 t
ORIGIN      
        1 cagtcgaagc cgttctggaa gcgctgcttc ttgccgagca tcgcgttgta gcgctggaag
       61 acatccttgt aggtgcggcc ccagccgtga tggacaccca tcggattgtt ggcggtgatc
      121 gggccgtcga tgaacgagaa acgttcggat gagccgtcgt tgcgatgcag gtattgctcg
      181 aggatctcgt gggccttcca ccactcgatc aggtcgcgtt ccatggcggg gaagtcgacc
      241 ttcgtcgata cctcgttgaa tgtgcgtgtt cttgtatcgg tcacgttcga tctccccgga
      301 ttgcgagtgt cgcaacgacg gtcctggacg aatgatggca acaaaaaagc ccgcccctag
      361 atagaggacg agcaaagccg cgataccacc tcagttggcc cggactcgcc gaacccacct
      421 cagacccgaa cgagtctcct cgccggatca tttccgcggt atcgggcgga ttccgatcgc
      481 gcctacttgg gatcgggaga tcccgttggg acgatggctc gggaaggatg tccgcttgca
      541 gttatcgaga ttcggcttcc atcgccccga aatcgctgga ctcacactgc agtggcgcgt
      601 tttccgtcaa cgccatgaat cttcgtttgc tttctgaatt atagcagttc gcgtcccgtt
      661 actggctact tgcggttgcc ataaagcgtc gctatccctg cgcgtggcga ccgagggcca
      721 ggcgaacctt gcggtagagg ttggtgagaa gaatgagata ggtgccgccg agggtatagg
      781 aggcgaggac atcggtcagc cagtggtggc cgagatatat cctgctcggc ccgacgagcg
      841 cgatcaggct cccgaggccg ccaacgatcg cgcgtctcca cttcttcggc ttgatccaac
      901 tgaaggcgag gaaggtcagg aagccgtaga caccgacgta attgatgacg tgaccgctcg
      961 ggaagctgct ccccccgatg tttgccttga tcacccggat cccggcgtca tccggtccag
     1021 gtcgggaccg ccgcatgatc ctcttgaccg tgaacgagat gccgccggtt ccccaggcca
     1081 tgagctggaa gaccgcctcg aggcggaatc cccgcgccca aaggatcgct gcgagcgatg
     1141 gcgggagcag gcggctctgc ggtgggaagc cgggccagga gacgagatgc atcaaccgat
     1201 cccagagcgg atgatccttc gcctggagcc tggtcgtgat ccgggcatcc agtttcgagc
     1261 ttcgcttcgt acgtacgatg aagaagaggc aggcgaagat gccgagcagc acggtcgcga
     1321 tggagatgcc ccgcttccct cgtgtgaacg ccagctgatc cggatcgagc cggtcacgaa
     1381 gaaaccaccg cgaagtcgtc ttcgaatcgg aggagagagc catcgtcgcc gcagccagcc
     1441 ttcactaagt gacgcggtcg aaccgagccg cgcgtcgcgt ttccatcaac ggaacgtttg
     1501 tcatcacgaa ccggacaccg taccgggcca agggtacact ttcgacgaac gtcgccaaac
     1561 cctcaggact tgcattcatg tcgcgaccag tcatccagac aatccgaacg tattggctct
     1621 ggtgcgcgat tgtcgccgcg gcagtggtcg tcttcctcgc gagccctggg gcgacgggga
     1681 acaaggcgca ttcggtcctc cacggactct gcgcgcagac gccgtcgcac acattcacct
     1741 tcggcgggca gccgctgccc ttcgacggtc ggatgacggg gatctacggt ggctcgatcc
     1801 tgacattcgc ctggctctgg attcgtggtc gcgtgctgtt ctacggaaac ccgcccaaac
     1861 gcgtcatcgc cgtgctcacg ctcttcgtga tcgcgctcgc ggtcgatgga ttcaactcgc
     1921 tctttcacga tctggggatc tggtatccgt atgagcccca gaacatctac cggctgatta
     1981 cgggattcgg gaccgggatg tcgctctcga tcatcctctc ctggctcctc gcgtcctcga
     2041 tgtggaacct cagtcgtccg accacgggga tcaggtccct gcgggatgtc ggcgcgatcg
     2101 cgcttctcgc gattccctat ggggcggccg ttctttcggg atggtcgtgg ctctaccttc
     2161 ccttgacggc gctgctcctg ggatccgcct ggttgaccct gagcatcctg atgctcgtgg
     2221 tggtcctcct cctcttccgg atcgacgaga aggtgcgtgc cgttccccag cttcacctcc
     2281 ccggtgcggc tgcctcgatt ctcggattgg tcatcatgtt gacgcttgcc ggtggtcgat
     2341 tctggctcga gcacaccttc ggcatcaccg tcgatatctc ctgatcgctc cgaattcgcc
     2401 cggatttcgc atggtagact ttggcgatag ttcccagaga ttcccacggt aacgacaccg
     2461 gttggcgaac ggctgggcga ccacttgtct tcacgacagc cctcaatcct ggccgggtct
     2521 cgccaggact gcaaaggact ccatgcgcat catccgtcac accacctata tccagcagcg
     2581 gaagcgccgc gcgcggggaa tcgcgttcgt cggctttgcg gtgctggcag cctcgctctg
     2641 gatcgctctc aatccgagcc tgctgattcc ggcctacgga gcgatgttcg tcggcttcat
     2701 caccttcaac atcggcatgc agcaggtcgg gaagtggtcg cgaaatcccc gaaatgacca
     2761 gatgctcgat cgtcagctcg agcgactcgg cgagcgctac acgctcatcc actttccgcc
     2821 cgtcggcaag cacaaggcgg aacacgtcct cgtctatccg gggggactgc tcgcgttgac
     2881 cgcacgggag atcgacggga aggtcgagac caccggcaac aagtggcgga agcgaagcgc
     2941 ggggctgcga cgaatcttcg ggttcagcgg tccgcaactc gggaacccga gctcggacac
     3001 cgacgcggcg ctgggtgagc tggagacctt cctcgaggag catcagttgg agctcgatgt
     3061 cgaagggatc atcgtcttcg tgcatccgct cgtcgaactc gaagtcgagg agcccgaata
     3121 tccggtactc cacggcgagg aagttccctc ctttgtccag agcctcccgg ccgacgagtc
     3181 cttctcgacg gcggagcgcg acgcggtcgt tgcgctgctt ggcgccggtg aggtcgtcga
     3241 agcgccgacc cgacagacgc gacggcgccc ggtcaagcgc cgcgccgcgt agagtcgaac
     3301 gacagccatg gaacgcagtc aatcccgcga gaattggggc gtctggggtc acgggcgaac
     3361 cgtccgcgac ctgcagcgtg ccgcggcgtc gagtcctcgg cactcgtaca tcatttccgg
     3421 tccggcgtcg gccgggaaga agacgattgc cctggccttc gcgaaggcac tgtcctgcgc
     3481 aagtcctccg gcgcccggtc tcttctgcgg cgaatgctcc gcctgccgtc gcatcgaccg
     3541 cgggacgttc ccggatgtga cgttcgtcga tctcgaaatg cagtcgagcc gcgagaagag
     3601 cacgtcacgc aacacgtcgc tcaatatcgc gacggtccgg gagatctcgg cgaatgtgat
     3661 gttgcgaccg ctcgaatccc ggtggcggat cgcgatcgtc gatgatgtgg agacgatgca
     3721 ggagacggcg caggaggcat tcctcaagac gctcgaggag ccccccgcct atacgatcct
     3781 cctccttctc acctcggacg ccgatctcct gctgccgaca atccgttcgc gctgcctgac
     3841 cctgtcgctg caagcggtcc cggtgaaggc aatcgcggat gggctcgagt cgattggaac
     3901 cgccagcgac gaggcggggg tcattgccga ggcgagcgcg ggtcttcccg gctgggcgat
     3961 tcgcgccgcc gccgacccgg ccctgctcga gtcgcaggtt gccgccgccg gtgaggcgca
     4021 cgcctgggcc cggtccggag agtacgagcg cctggtaacg gcaacccgta tcgcggacca
     4081 gttcctcaag gaccgcgagg ccgtcttctc gcgactcctt accgttcagc gcgcctggcg
     4141 cggtctgctt cacgatgcgg tgaacggcga tgcctccgtg agcgcggaga gttccgtgac
     4201 cgccctgaag agcatcgaga cgtgcctgct cgacctcgaa tccaacgtcc ggccccgcct
     4261 cgcgctccag cagatggtgc tccaatggcc ggtcattcga tgaaccgagt cggggctcgg
     4321 accgtgatcg gcgtgcggat ccagcacgag acgccggtcc gtttcgtcgc cgtcgatccc
     4381 gacctcgccg gtgccgcgcc atacacctgg cttgtcgtcg agcgcgcggg ggaggaactt
     4441 ccggcgatcg tcgtgatccc ggccgagctg atggtcg
//
LOCUS       DASTMD010000236         2942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3057677, whole genome shotgun sequence.
ACCESSION   DASTMD010000236 DASTMD010000000
VERSION     DASTMD010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3057677"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1023)
                     /gene="map"
                     /locus_tag="VFQ54_03560"
     CDS             complement(280..1023)
                     /gene="map"
                     /locus_tag="VFQ54_03560"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU0164094.1"
                     /translation="MVIVRRTEQLEGLQRANRETTRMLRYLQTVAQPGVTTKVLDDAA
                     RAFIAELGGEPVFATQAGFPGAINANPNDVVVHGVPGKYVLQKGDIFTIDGGMLLDGY
                     VGDAAITFPVGPMTPRHQALIDTTWGAMQAAIAAAKTGAHVGDVSWAMQSYAEEHGCN
                     VVRDFCGHGLGTRMWEDPQVPFAGNPKTGERLLEGMVITVEPVVMEGQMDYYMANQWE
                     ARSVDGKWVAQFERAVMVTKSGGVILSGD"
     gene            complement(1184..2185)
                     /locus_tag="VFQ54_03565"
     CDS             complement(1184..2185)
                     /locus_tag="VFQ54_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU0164095.1"
                     /translation="MNPIVVNGPSGMVARGPQDRLTTTRRPYRLVTYVSLVAMLFAGF
                     FAIPGVARAASDQTTSSVNFRTGPGTDYPSMTVIPSDASVEILGDPENGFYPIRYNDA
                     TGYVSSDYLSTGTSDADSGGDAGSSDVQSGGPTGTAYVIDGALNLRDGASLSASVILV
                     MPGDATVTLNGDSSNGFYGVTYKGTDGWASADFISQDAPAAETPAPTDPPVAETPAPT
                     DPPTVKTPVPTQAPATEAPVDNGNSGSTDIVSIIYAAADRYGQSRADMLRVATCESNL
                     DPNAVNPSSGTSGLFQFMPSTWASTPYADQSIFDAYANANAAGWMWSVGRRGEWQCQ"
BASE COUNT          555 a          977 c          891 g          519 t
ORIGIN      
        1 ggatgttttc cggggggaca tagaagtccc cccgtactca cgaccgccgc acgaacggtc
       61 tcggtgacaa gaagcgttac cccgaatgac gttggagacg atcggggtct gtagggcgag
      121 cgttcatctc tcgccgcaag cccgacaccg atgcgacgcg ttccgcgatc cacaatcgtt
      181 cccgaacgaa tcggcggagt tttcccgagc gccgtcgaat gggtcggctc ggaacggcga
      241 cgagcgccgt ggggacggat ttttcgggac gagatacgcc tagtcccccg agaggatcac
      301 accgccagac ttcgtcacca tcacggcgcg ctcgaactgc gcgacccact ttccgtcgac
      361 gctgcgtgct tcccactggt tggccatgta gtaatccatc tggccctcca tcacgaccgg
      421 ttcgaccgtg atgaccattc cctcgagaag ccgctcgccc gtcttcggat tgccggcgaa
      481 gggcacctgc ggatcctccc acatgcgcgt gccgaggccg tgcccgcaga agtcacgcac
      541 gacgttacag ccgtgctcct ccgcatacga ctgcatcgcc cagctgacat cgccaacatg
      601 ggcgcccgtc ttcgccgcgg cgatcgccgc ctgcatcgca ccccacgtcg tgtcgatcag
      661 cgcctggtgg cggggcgtca tcggtccgac cgggaacgtg atcgcggcat cacccacgta
      721 accgtcgagc agcattccgc cgtcgatcgt gaagatatcg cccttctgga gcacatactt
      781 cccgggcacg ccgtgaacca cgacatcgtt cgggttggcg ttgatcgcgc cagggaaccc
      841 ggcctgcgtc gcgaagaccg gctcccctcc gagttcggcg atgaacgccc gcgccgcgtc
      901 gtcgagcacc ttcgtcgtca ccccgggctg cgcgaccgtc tgcagatacc ggagcatgcg
      961 cgtcgtctcg cgattcgcgc gctgcaatcc ctcgagttgt tctgtccgcc tgacgatcac
     1021 catgatctcc cggaatcggg cgccttatgg cccggcttca actatctctg cctgacatcg
     1081 ttccataaac agctactccg gcgcaagcga tcgagtaacg agaatggggc agcatcacag
     1141 tgatgccgcc ccatttctca tcccaggttc ctctttctgg aggctactgg cactgccatt
     1201 cgccgcgtcg tcccacggac cacatccagc ccgcggcgtt cgcattggcg tacgcatcga
     1261 agatgctctg gtccgcatac ggggtcgacg cccacgtgct cggcatgaac tggaagagtc
     1321 cgctcgtccc gctgctcggg ttcacggcgt tcgggtcgag attcgactcg caggtcgcga
     1381 cacgaagcat atccgcccgc gactgaccgt agcgatcggc ggcggcgtag atgatcgaca
     1441 cgatgtcggt cgaaccactg ttgccgttat cgaccggcgc ctcggtggcg ggagcctgcg
     1501 tcggaaccgg agtcttgaca gtcggcggat cagttggcgc cggcgtctcg gcgacaggag
     1561 gatcggtcgg agccggggtc tcggcagccg gtgcatcctg gctgatgaaa tcagccgagg
     1621 cccagccgtc cgtgcccttg taggtcacgc cgtagaaccc gttcgagctg tcaccgttca
     1681 gcgtcaccgt cgcatcaccc ggcatcacga ggatcacgct cgccgagaga ctggcgccgt
     1741 cacggagatt gagtgccccg tcgatgacgt acgcggtacc agtcggtccg ccgctctgga
     1801 cgtcgctgct cccggcgtcg ccaccgctgt ccgcgtcgct cgtcccggtc gagaggtaat
     1861 cgctggagac ataaccggtc gcgtcgttgt agcggatcgg gtagaagccg ttctcgggat
     1921 caccgaggat ctcgaccgac gcatcggacg ggatgacggt catgctcggg tagtcggtgc
     1981 ccggaccggt gcggaagttg accgaactcg tggtctgatc ggaggccgcc cgcgcgacgc
     2041 ccgggatcgc aaagaatccg gcgaagagca tcgccacgag gctcacgtag gtgacaaggc
     2101 gatagggacg acgagtcgtc gtcagacgat cctgcggtcc acgagcgacc atcccgctcg
     2161 gaccattcac aacgatggga ttcaagaagt gctccttctc ccctcgtcgg cgacacacat
     2221 gcgcgcaccc gatgacggcc tggaattgac acaacatctc gtacgcgcgg tcttcagagc
     2281 tggacatccg gatcagccaa atcctggaat ccatcttttc ccaccgaaaa cggcgcagga
     2341 ttaccctgat cgtctcgcgg gagccgaaga acgtgtcaaa ccgattgttg gggcacggtg
     2401 cctgatcgga aaagtagatg tgaccccgcg acactcgtcc ctccaacggt gccgggttgc
     2461 gcctccgaac cttgtcaaac gatcgcggtc agccaccgaa atcgcgagtt cgaacgagcg
     2521 cgtcgcacaa acgaaaacgt gtctgtacga gcagtacggt cccatcctcc agccacggac
     2581 gttcgggacg tcgctcataa gcacacactt atgccacagc gacacagggg tgtcaacaag
     2641 gcacgccgcg gtggcccttt gtgacaacaa gatcacttag gattgaatca tgggtttacg
     2701 ggatgaccgt agcccggtcg caagcttcgt acgacgaggt ccacgccgta accgcgccgc
     2761 gatacccgcg tacgtacgaa cgttgcgggc gactcacagg actatgacga ccgcagatga
     2821 agcggtccca gtcgggacgg tgaaccaatt cccggaagac ccaggacctc cggtggccag
     2881 ggctcatccg cgggaaggag gaggcgccgc ccgcacccga gccggcgcca ccgtgccgat
     2941 ga
//
LOCUS       DASTMD010000237         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_379479, whole genome shotgun sequence.
ACCESSION   DASTMD010000237 DASTMD010000000
VERSION     DASTMD010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379479"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VFQ54_03570"
     CDS             complement(<1..704)
                     /locus_tag="VFQ54_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0164096.1"
                     /translation="MTLASSASALKVPIAAAPVTWNNNDLENWRPRIPFPDILDRIAG
                     AGYTATEYDGSFGTDSAIVTTEAGKRGLVFCGSYQWFDLQTKGDVIAETLNLVSTLEL
                     LQAIHCEHLIVADALRPERVAIAGAVPADGSAALGDDGFARIASNAHRVAAIAAGYGI
                     RSHYHNHVGTYVETPDEVESLVGELDLAQVDLCFDTGHFAFGGGDALRFVEAHLDTIG
                     YVHLKDVNATVLAEAKA"
     gene            complement(760..1365)
                     /locus_tag="VFQ54_03575"
     CDS             complement(760..1365)
                     /locus_tag="VFQ54_03575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0164097.1"
                     /translation="MDSQYSVPLTPIEAKIITALQQEDHAQREANLPIEARTRNISAA
                     TGPFLWQMVVGHKPRTILEIGSSTGLSTMWLASAASRIGARLLGTEVIVERAAQNNAQ
                     LRRAGLDGTARVADSDHRNVDDVKSTAWEFVFLDAEKDDYLAHLQAIEPYLAPGAVIL
                     TDNVISHDCTELQNYLRGSAKYSTITIPLDRGIEYSVLLPQ"
     gene            complement(1358..>1577)
                     /locus_tag="VFQ54_03580"
     CDS             complement(1358..>1577)
                     /locus_tag="VFQ54_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU0164098.1"
                     /translation="KGIEQLGLTAGEVVMVGDRLDTDILAGHRVGMTTALVLTGISTR
                     EEIATAPAIPDYVFADLPAITQEIVGHG"
BASE COUNT          284 a          466 c          529 g          298 t
ORIGIN      
        1 gcctttgcct ctgccagaac cgtagcgttc acgtccttta ggtggacata accgatggta
       61 tcgagatgtg cctcgacgaa gcggagagcg tcaccgccac cgaaggcgaa atggccggtg
      121 tcgaagcaga gatcgacctg ggcgagatcg agttccccga ccagggactc gacttcgtcc
      181 ggcgtttcca cataggtgcc gacatgattg tgatagtggg agcgaatccc gtagccggcg
      241 gcaatcgcgg ctacccgatg tgcattcgag gcgattcgcg cgaagccatc atcgccgagg
      301 gcggcactgc cgtcagcggg cacggcgccg gcgatcgcga cacgctccgg gcgaagggcg
      361 tcggcgacga tcaggtgctc gcagtggatc gcctggagca gctccagcgt agaaacgagg
      421 ttcagcgtct ccgcgatcac gtcccccttg gtctggagat cgaaccattg gtacgacccg
      481 cagaagacga gaccgcgttt ccccgcctcg gtggtgacga tcgcagaatc ggtcccgaaa
      541 ctgccgtcgt attccgtggc cgtgtagccg gccccggcga tgcggtcgag gatgtcggga
      601 aagggaatac gggggcgcca gttctcgaga tcgttgttgt tccaggtcac cggcgccgcg
      661 gcgatcggga ccttgagggc ggacgcggat gacgccaatg tcatgaaagc ctcctggagc
      721 tcgagttgtg gaaactggca atccgtacgt ccggatcggc tattggggga gcagaacgga
      781 atattcgatg ccgcggtcga gcggaatcgt gatcgtcgaa tacttcgccg aaccgcgcag
      841 gtaattctgc agttcggtgc aatcatgaga gatcacgttg tcggtcagga tgacggcgcc
      901 cggtgcgaga tacggctcga ttgcctggag atgcgcgaga tagtcgtcct tctcggcatc
      961 gaggaagacg aattcccagg cagtggactt cacatcgtcg acgttccgat gatcggaatc
     1021 ggcgactctc gcggtgccgt cgaggcctgc ccgtctcagt tgggcgttgt tctgcgcggc
     1081 ccgctcgacg atgacctccg tcccgaggag tcgggcgccg atgcgggatg cggcgctcgc
     1141 cagccacatc gtggacagcc ccgtcgagga tccgatctcg aggatcgtcc ggggcttgtg
     1201 accgacgacc atctgccaga ggaagggacc ggttgcggcg gagatgttcc gcgtgcgcgc
     1261 ctcgattggc aggttcgctt cccgctgcgc atgatcctcc tgctgcagtg cggtgatgat
     1321 tttcgcctcg atcggcgtga gcggcacgct gtactggcta tccatggccg acaatctcct
     1381 gcgtgatcgc gggaagatcc gcgaatacgt aatcgggaat cgcgggggcc gtggcgatct
     1441 cctcccgcgt ggagatgccg gtcaggacca gtgcggtcgt cattcccacg cgatgcccgg
     1501 caaggatgtc ggtatcgaga cggtcgccga ccatcacgac ctccccggcg gtgagaccga
     1561 gttgctcgat tcccttc
//
LOCUS       DASTMD010000238         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1360530, whole genome shotgun sequence.
ACCESSION   DASTMD010000238 DASTMD010000000
VERSION     DASTMD010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360530"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            881..2080
                     /locus_tag="VFQ54_03585"
     CDS             881..2080
                     /locus_tag="VFQ54_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0164099.1"
                     /translation="MTRRSLLFGIGGTGVMLGLAGCGGSGSDDKPTVTPEPTSTLEPT
                     QAPPTPTQAPISSPVPGYLDPTRWEGRTITVASAAAGDYLDALSAAYFDAFATATGAK
                     VRHTDFGRDGINGIGNQVKSAEIIWDVVLMPTENALPLAQKGYLTAIDYNVVDASNLY
                     PELAIQHAVGQAIYSTAMVYSSTASKPPTGWKDFWDLSLFGGQRALRKDPVGTLEFAL
                     LADGVEMKNLYPLDTARAFAALDRIRDATVFYEDSKQPVELVRSGQVGLASAWSARTD
                     LPDVASLVRTQWDGGMLCADSWGIPKGSANTDIAMSFINFATRAVPAASFTRLQPFGP
                     VNKQAFALLRDDVVAGLPNAPQNIGGQFFQDWSYWAGLQDDLTNQFNDWVLNPTGSPV
                     PSSTPAQ"
     gene            complement(2240..3826)
                     /gene="ggt"
                     /locus_tag="VFQ54_03590"
     CDS             complement(2240..3826)
                     /gene="ggt"
                     /locus_tag="VFQ54_03590"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU0164100.1"
                     /translation="MSRPTTLATNGMVASPHYLASAAGLRVLERGGSAMDAAIAANAV
                     LTVVYPDQTAIGGDCFFIAYDAASGKTFAYNGSGPASNHADPDGLRGQGYTAMPAKGG
                     PTVTVPGTIDAWFAGHDRFGKLEMSELLSSAISLARDGFPVSSRLAAVFQRDVDLINS
                     WPGLAAIFFAGGTIPAAGDRLRLTALAASLQKIATNGRDVFYGGHIADEISRSVQAAG
                     GWIATDDLAGYKGEWVDPVRTTYRGVDVLTIPPNSQGVTVQIALKLIEGIDLRSLTWG
                     SADHIHPLVEARKRAYAVRDAKFGDPRFIDIDLGTLLSDSAIANLWADYNPELAAAGP
                     YTIAGDTVYLCAVDRDGNAVSLIQSLFSNFGSAVVAGDTGIILQNRGSFFSLVEGSPN
                     ELLPGKRTLHTLMPNMLMKDDRLFGPIGTQGGDPQAQINLQLMTNVIDFGMEPQVAIE
                     SPRWISGGPGGTLPYQLLLESGFPDSTPALLGARGHEITLIGPWNPDAGHAQMILVDE
                     ARGVLQGGADPRADGTADGY"
BASE COUNT          757 a         1317 c         1251 g          711 t
ORIGIN      
        1 gatccgcacg cgaatctttt cgccgtcgat cgcgtgaatg aaggtcgcct cggtggccga
       61 tttcggaata gcatcctgag ccgccaccgt ggaggagagg acgaatggtg tcgctgcaat
      121 cacagcggat ccagcaacga atgatcgacg agacatgtgc accaccacag ggctcctttg
      181 ttaatgcctg accatcatgg tcaagtcatg ggcggatgaa gtttctcact atctcaatat
      241 ttacggtatc cccagtctta atgtctcttt cgatcctcgc tcgcgcattg ctgtcccatg
      301 tcagcatacg gcaatgggtc tccgcccatt caaagtgtcg tggtggaggt tcgggacatt
      361 ccgatcgttc cccaatcccg tccgtcgcga gacatccgta acatccgagg cgccgttgcg
      421 tctggcgact caccatccga ggaaccgaca gaaatcgggg ttcggcagcg tctggacgtc
      481 gcatacaggt cgtcgacgcc agaggcgcga cggtcaatag gccattctgg gtcaatcgcg
      541 ttcggacgtc cgatcttact catctccacg ccccgtcgga agcaggtcga cgacgaaggc
      601 atccggttga agcgcagcac gatactgtcg tcactgccga actgcccaca ccgccgttga
      661 ctatcgctta tcattcgcac ctgagacgca ccgtagaccg ggtaagccac ggcgacgggt
      721 atcgttcatg cagtagggcg atcagctatg cgagaaggcg gcatcgtccg cttcaaacat
      781 ctttctgcgc acaacacgaa tcaacacgac atatgaatcg aacatcatga ccgcttccgc
      841 acgacgggac aacgaccgtc ccgaccgatc atcatcgcgc ttgacgcgtc gatccctcct
      901 cttcgggatc ggcggtactg gcgtcatgct cgggctcgcc ggttgcggag gatcgggttc
      961 ggacgacaaa cccaccgtga ctccggagcc aacgtccacg ctggaaccaa cgcaggcgcc
     1021 gccgaccccg acgcaggcgc cgatctcgtc gcccgtgcct ggctacctcg acccgacgcg
     1081 ctgggagggc cggacgatca ccgtggcgag cgcggccgcc ggggattacc tcgatgccct
     1141 gagtgccgcg tacttcgacg ccttcgccac ggccaccggc gcgaaggtcc gccacacgga
     1201 tttcggtcgc gacggcatca acggcatcgg aaaccaggtc aagagcgccg agatcatctg
     1261 ggacgtcgtt ctcatgccga ccgagaatgc gctgccgctg gcccagaagg gctacctgac
     1321 ggcgatcgac tacaacgtcg ttgacgcgag caatctctat ccggaactgg cgatccagca
     1381 cgcggtcggg caggcgatct attcgaccgc catggtctac tcctcgaccg cctcgaagcc
     1441 gcccaccggc tggaaggatt tctgggatct gagtctcttc ggcgggcagc gcgcgctgcg
     1501 caaggatccc gtcgggacgc tcgagttcgc cctgctcgcc gacggggtgg agatgaagaa
     1561 tctctatccg ctggacacgg cgcgcgcctt cgccgcgctc gaccggattc gtgacgcgac
     1621 cgtcttctat gaggacagca agcagccggt cgaactcgtg cgcagcgggc aggtcggcct
     1681 cgcgagcgcc tggagtgcgc gcacggatct ccccgacgtc gcctcgctcg tgcggacgca
     1741 atgggacggc gggatgctct gcgccgattc gtggggaatc ccgaagggat ccgccaatac
     1801 ggacatcgcg atgagcttca tcaatttcgc gacgcgggcg gtaccggcgg cctcattcac
     1861 acggctgcaa ccgttcggcc cggtcaacaa gcaggcattc gccttgctcc gcgacgatgt
     1921 cgtcgccggt cttccgaacg cgccccagaa catcggcggc cagttcttcc aggactggag
     1981 ctattgggcc ggcctccagg acgacctgac gaaccagttc aacgactggg ttctcaatcc
     2041 aaccggatcc cccgttccct catccacccc ggcgcaatag cgaacactcg tcaggtgcag
     2101 tgacggaccc ccccgggaat ccacgaattc gtggatcggt cacggctttg cccaggacat
     2161 ccggtcgttc gggagccttc cctgagcttg ccgaacgggt ccggacggcc cgtcccgcat
     2221 ccacgattcc cgcggtgcct caataaccgt ccgccgtgcc atccgcccga ggatcggcgc
     2281 caccctgcag cacgccacgc gcctcgtcga ccaggatcat ctgggcgtgg ccggcgtccg
     2341 gattccatgg tccgatcagg gtgatctcgt gtccgcgcgc tcccagcaag gcgggcgtgg
     2401 agtccgggaa gccggattcg agcaggagct ggtagggaag cgtgccgccc ggtccgcccg
     2461 aaatccagcg cggggactcg atcgcgacct gcggctccat gccgaaatca atcacgttcg
     2521 tcatcagctg gagattgatc tgggcctgcg gatcgccacc ctgtgtcccg atcggtccaa
     2581 acaggcgatc gtccttcatc agcatgttcg gcatcagcgt gtgcagcgtg cgtttgcccg
     2641 gaagcagttc gttcggcgat ccctccacca gcgagaagaa cgagccgcga ttctggagga
     2701 tgatccccgt gtccccggcg acgacggccg aaccgaagtt gctgaacagg ctctggatca
     2761 gggagaccgc gttgccatcg cggtcgacgg cacataggta gaccgtgtcg cccgcaatcg
     2821 tgtatggacc ggcggcggcg agctcgggat tgtaatccgc ccacaggttc gcgattgcgc
     2881 tgtcggacag aagtgtcccg agatcgatgt cgatgaaccg gggatcgcca aacttcgcat
     2941 cccgaacggc gtaggcgcgc ttcctcgcct cgacgagcgg gtggatatga tcggccgatc
     3001 cccaggtcag cgaccgaaga tcgattccct cgatcaattt gagcgcaatc tggaccgtca
     3061 cgccctgcga attgggtgga atcgtcagta catcgacgcc acgatacgtg gtccgcaccg
     3121 gatcgaccca ctcgcccttg tatccggcga ggtcgtcggt cgcgatccag ccgcccgcgg
     3181 cctggaccga cctcgagatc tcatccgcga tgtgaccgcc gtagaagacg tcccgaccgt
     3241 tcgtcgcgat cttctggagc gacgccgcca gcgcggtgag gcgcagccga tcgccggcgg
     3301 cggggatcgt gccgcccgcg aagaagatcg ccgccagccc cggccaggag ttgatcaggt
     3361 cgacatcccg ctggaagacc gcggcgagac ggctcgaaac gggaaacccg tcccgcgcca
     3421 gcgagatcgc ggaggatagc agttcggaca tctccagctt gccgaagcga tcgtggccgg
     3481 cgaaccaggc atcgatcgtg cccggcaccg tcacggtcgg tccgcccttg gctggcatcg
     3541 ccgtgtagcc ctgcccacgt aatccatccg gatcagcgtg attcgaggca ggaccggagc
     3601 cgttgtaggc gaaggtcttg ccactcgcgg cgtcgtaggc gatgaagaag cagtcgccgc
     3661 cgatcgcggt ctggtcggga tagaccacgg tcagcacggc gttggcagcg atcgccgcgt
     3721 ccatcgccga cccgccgcgc tccaggacac gaagcccggc cgcactcgcc agatagtggg
     3781 gactcgcgac cataccgttg gtggcaagcg ttgtcggtcg actcatgggc atggttggat
     3841 tctccggtgg gatggcgatg gatcggcaaa gcggcggctt gcgaggacgt gcacggacga
     3901 cgctttcggc aggaggaaac gcacggggcc actgattcgt agataatact aggaggagtg
     3961 aatcgtcgaa cccgtgtgcg gcactcgccg aaacgattcg gcggcgctgg tcgtcgcacg
     4021 aacatcttga tcatgc
//
LOCUS       DASTMD010000239         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_826299, whole genome shotgun sequence.
ACCESSION   DASTMD010000239 DASTMD010000000
VERSION     DASTMD010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826299"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VFQ54_03595"
     CDS             complement(<1..1430)
                     /locus_tag="VFQ54_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEU0164101.1"
                     /translation="MTALRVLAALTLLLTGWFGAVTSTSAQSDSSVSATVTTANSVPS
                     LTYFALGDSYASGFGLLGDDNTHCDRSSNAYPYLVRDSLEQTYSTVDLRHLACAGATA
                     GTNTTDTSDPDKSISHQFASANAYLDQRDGITNPDPVLVTITIGANDIGLTDPTTLGQ
                     IIRPLSAIQPTVIAPDTVRIGLPQSGVDFFAWLDKKTAPIAPSLTAGIDTLLSHKNVH
                     IVLTDYPDPFRQGGFGACNDFFRSLSCDDAMNFLILRLNSIVLDQLIRIKDPGRLRVA
                     ALGPSFASHTADCAPFATSANAAETWFQQPDKDDPASNIVACVHPNQLGAQGIADAIL
                     ELVPTMLPAPVEAPPRTWTASVQVSLCDAPPNSGQDMNCQGTTGVVVNVSLASGEPLG
                     SCTSGEPEQFPGGDMISLCSIDGLPFNADLVASQDLSTVPAGYEPFSNSLTLHVDDLI
                     SGGGDQATFTFIDVPPDASGSAGVTSG"
     gene            complement(1725..2213)
                     /locus_tag="VFQ54_03600"
     CDS             complement(1725..2213)
                     /locus_tag="VFQ54_03600"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEU0164102.1"
                     /translation="MGALFNFGGRVGRSAYWGVIAATVALYAGYIVWAANLAPWERIQ
                     VYTSYSYYSGDSSTQISWGSDDAKPIYICFAIAAAVLLLISLSVSARRWHDLGKSAWW
                     SLISIVPIVGSLYVFVMLGFVAGSEGPNRYDDARRPGGIPVPSHAGEEPSPTPWWWKE
                     ES"
     gene            complement(2633..>3067)
                     /locus_tag="VFQ54_03605"
     CDS             complement(2633..>3067)
                     /locus_tag="VFQ54_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU0164103.1"
                     /translation="ASDNEQSGTADLAVDGNPSTMWMVTSSPSQQTVELVVDLGQLQP
                     VGGVSWWLGTTGTMPAYQVWLSDDGQTWHLQSSFEGSVQHPQLWWGDGVGFWARYVKF
                     LVPHADQSGLQQIGGISEIQIWRTPDGNAMSLQYYVTPVAGP"
BASE COUNT          611 a          946 c          949 g          561 t
ORIGIN      
        1 ccggaggtga cgccggccga tccagaggcg tcggggggta cgtcgatgaa ggtaaaggtt
       61 gcctggtctc cgccgccgga gatcagatca tcgacgtgaa gggtcagcga attactaaat
      121 ggctcgtagc cagcgggaac ggtagacaga tcctgcgaag cgacaaggtc cgcgttgaat
      181 ggcagtccgt ctatggagca gagcgagatc atatcgcccc ccggaaactg ctccggttct
      241 ccgctcgtgc atgatccgag gggttctccg gacgcaagcg acacgtttac caccacaccc
      301 gtcgtgccct ggcaattcat gtcttgaccg ctattggggg gtgcatcaca gagcgagacc
      361 tgaacggagg cggtccaggt tcgcggcggg gcctccacgg gagcgggaag cattgtaggc
      421 accaattcga gaatggcatc agcgatccct tgggcgccca gttgattggg atggacgcag
      481 gctacgatgt tggaggccgg atcatccttg tccggttgtt gaaaccacgt ctcggcggca
      541 ttggcgctgg tggcaaaggg cgcgcaatcg gccgtgtgag atgcgaagct gggtccgagc
      601 gccgcaacgc gcaagcggcc gggatccttg attcgaataa gctgatcgag cacgatcgag
      661 ttcagtcgca agatgaggaa gttcatcgcg tcatcgcagg aaagcgacct gaagaagtcg
      721 ttacaggcgc caaaaccccc ttgccgaaac gggtccgggt agtccgtaag cacaatatgg
      781 acattcttgt gagacagcag agtgtcgatc cctgctgtca aactgggagc tatgggcgcg
      841 gtcttcttgt cgagccaagc gaaaaagtcg acgccggact gggggagacc aatcctgacg
      901 gtgtcgggcg ctatcaccgt gggctggatg gccgataacg ggcggataat ctgccccaag
      961 gtcgtgggat cagtcagccc aatatcattc gcaccgattg tgatcgtcac gagcacggga
     1021 tcggggttgg tgatcccgtc ccgctgatcg aggtatgcgt tcgcgctcgc aaactgatga
     1081 ctgatggatt tgtcaggatc ggacgtatcc gtcgtgtttg tccccgccgt ggcccctgcg
     1141 caggcgagat ggcgcagatc gaccgtgctg tacgtttgtt ccaggctatc ccggacgagg
     1201 tacggatacg cgttggagga ccggtcgcaa tgtgtattgt cgtcaccgag tagaccaaaa
     1261 ccggatgcgt acgaatcgcc caaggcgaag taggtgagag acgggacgct gttcgccgtc
     1321 gtcaccgtcg cgctgactga gctgtcgctc tgcgcggaag tagatgtcac cgcgccgaac
     1381 cacccggtga gcagcagcgt cagcgccgcg agcacccgca gtgcggtcac cacaacttcc
     1441 cttcgcagat ccctgtgcct caccggtctt tcctttcctt gacgaccgtt taccgccatg
     1501 tgatggcgct cccggttaat cgatccaact tcgggaccct tccgatgaga attgccagat
     1561 cgagccttcg gcaggggccg gtccatagtc attggcgccg tcgtctcccg gcacgaatcc
     1621 cagaaagcca agggtgatca acgacgcgac tgccctgttg gtggtgtccc gacgcagtga
     1681 tacccacacg ccgtgcattc ccgttctctc tgcgtgcatt ggtttcagga ctcctccttc
     1741 caccaccacg gggtcggcga cggctcttcc ccggcgtgag agggtacggg gatcccacct
     1801 ggtcgccgcg cgtcgtcata ccgatttggc ccttcagatc ccgcgacgaa cccgagcatc
     1861 acgaagacgt agagcgaacc cacgatgggg acgatgctga tcaggctcca ccacgcgctc
     1921 ttgccgaggt cgtgccaacg ccgggcactg acggagagac tgatgagcaa caacacggcg
     1981 gccgcgatcg caaagcagat gtagatcggt ttcgcgtcgt ccgatcccca ggagatctgc
     2041 gtgctcgagt cgcccgagta ataggagtag gaggtgtaga cctgaatccg ctcccacgga
     2101 gccaggttcg ccgcccagac gatgtacccc gcgtagaggg cgacggtggc cgcgatcacc
     2161 ccccaatagg ccgacctgcc gacacgtcca ccgaagttga acaacgcgcc catcgatctc
     2221 tctcctccct gatgacgatt ccaaccatcg tgtagacgtt cccggtgatt gcctccctat
     2281 cggagcgtga cccggatcca cgccccgctc ttgttcagat cgcgccgccg ccgaacgctg
     2341 accgcgagtc cgcggctggc aggtgaggta cacgacgagc gcaatcagga ccgcaacttc
     2401 gccgctcgcg ctcgaaagga aggcgaaaaa gatcgaggag atcaccagga tttgccgggc
     2461 aaagcttcat gcctcgacgc gagcgggctc gcctgagccg acagcgaacc catcctggca
     2521 gcaacccgaa acgcgctctg gattcggagg tgaacagcga gacccccttt cgggactccc
     2581 gttgcgatga acgctgccgt cgggactggc gccggcgtcg acgtacccgt ggctacggtc
     2641 ctgccaccgg cgtgacgtag tactggagcg acatggcgtt tccatccggt gtccgccaga
     2701 tctggatttc ggagatgcca ccgatctgct ggagcccact ctgatcggca tgtggcacca
     2761 ggaacttgac gtaccgcgcc cagaagccga ctccatcccc ccaccagagt tgcggatgct
     2821 ggacggagcc ttcgaacgac gactgcaaat gccacgtctg cccgtcatcg gagagccaga
     2881 cctggtaggc cggcatcgtc ccagtcgtcc ccagccacca gctcaccccc ccgaccggtt
     2941 gcagctggcc gagatcgacg acgagctcga ccgtctgctg cgagggagac gaggtcacca
     3001 tccacatggt cgacggattg ccatcgaccg caaggtcggc ggtgcccgat tgttcgttgt
     3061 cgctggc
//
LOCUS       DASTMD010000240         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2433411, whole genome shotgun sequence.
ACCESSION   DASTMD010000240 DASTMD010000000
VERSION     DASTMD010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2433411"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(444..812)
                     /locus_tag="VFQ54_03610"
     CDS             complement(444..812)
                     /locus_tag="VFQ54_03610"
                     /inference="COORDINATES: protein motif:HMM:NF039377.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6204 family protein"
                     /protein_id="HEU0164104.1"
                     /translation="METRRYRTIVRGRFRDLDEERQTALVADAAGHDALKAAFTGEGT
                     LTYDRTLKFFAFRYEFDVTGENAADCDLEAELVAEDRAIGDLAAREITHGPLTVSWTC
                     MSEMRTERRKAPKRPKAAAS"
     gene            complement(860..2224)
                     /locus_tag="VFQ54_03615"
     CDS             complement(860..2224)
                     /locus_tag="VFQ54_03615"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0164105.1"
                     /translation="MTAPEIASAAQESLDRWNVPGAAIGILKDGQTSFASFGVTSLET
                     QQPVTPETLFRVASITKPFTATLAMTLVNDGLLDLDRPIGAYLPGVTLTDGDPERQAR
                     ITMRHLLSHTAGIDCELAVDLATVGNGEDALREAIGFYGGLHQWAEPGQIMSYGNTGF
                     WLAGHVIATLLGTTFEEAMRERVYKPLGLERSVFTAEEAIVYPVALGHQPKSATDGAH
                     RLIRSYAYPRARTPSGGVISTVVDLLTFAQWHIDGTGGPLGLSDERRRSMQEPYIRLR
                     DADNESWGIGWTIRYTTDGTKVIGHGGSYSGFQTQLTIVPSRHFAFAILTNSARGSLP
                     NHEIERHVLREELGLALDAPRTQPIATERLETFAGFYRQPLAAYEVDAVGDGLEIVAR
                     NFDLTGKPMPVPPPIRFEPIGEHDFIAREGAFTGTRADFILDPETNAPAYVRIGLRVA
                     ERVA"
     gene            complement(2384..>2808)
                     /locus_tag="VFQ54_03620"
     CDS             complement(2384..>2808)
                     /locus_tag="VFQ54_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164106.1"
                     /translation="PVDDIQAAYRQWQVCKALGSTYQQMALETDQYIREAIYSDARTM
                     TAYTEPTINELLDRLEEADEEFGQLALSPGSVAGMSPRAIDVNGSVNASADGTYVFVD
                     IVKVIDNGDGTYTFNPDGEMSFQLVDGVWKIDLDGQLP"
BASE COUNT          456 a          870 c          969 g          513 t
ORIGIN      
        1 gaagcatctc ctgctgcggg cgcgggcggc ggaagttcac gacgatccga ccggcacgaa
       61 tccctcgcgg gacgagggac gtccgccgga ggtcgcgggc tcgctcggcc tcggccggat
      121 ggggtaggga tcagggagga atggttcccg cagacgggcg gcggacagac gggcggcggg
      181 acaggccgtc cagatctgcg atctggcagc ggcggtcccg ccctacgaac gaccgccggc
      241 gtcatcacca ggtaatttcg ttcgacaccg ctcacccagc gttcgttcgt agggcgggac
      301 tatggaggcg tacgaccgga gcacaacggt ccttgcgagc atgacgcccg gtcgttcgcc
      361 ggaagtatgt cccgcctttg ttacgcccga ggatttcgcg acgcatggag cgttcgtgtc
      421 gcggcaagac acaaggtctt gccctacgat gcggctgctt tcgggcgctt cggagccttg
      481 cggcgctcgg tgcgcatctc gctcatgcag gtccagctga cggtgagcgg tccgtgcgtg
      541 atctcccgcg ccgccagatc gccgatcgcg cgatcctcgg caacgagttc cgcttcgaga
      601 tcgcaatcag cggcgttttc tcccgtcacg tcgaattcat atcgaaacgc gaagaacttg
      661 agcgtccggt cataggtgag ggtgccctcc ccggtgaagg cagccttgag cgcgtcatga
      721 cccgcggcat cggcaacgag cgcggtctgt cgttcctcgt ccagatcgcg gaatctcccg
      781 cgcacgatcg tgcgatagcg tcgtgtttcc attgtcccaa ctccactcgt tggcccggcc
      841 agtccaggga gcgatcgcct caggcgacgc gttccgcgac gcggagcccg atccgcacat
      901 aggcgggcgc gttcgtttcg ggatcgagga tgaagtcggc gcgcgtcccc gtgaaggcgc
      961 cttcgcgtgc gatgaagtcg tgctcaccga tcggctcgaa gcggatcggc ggtggcacgg
     1021 gcatcggttt ccccgtcagg tcgaagttgc gcgcgacgat ctcgaggccg tcacccaccg
     1081 cgtcgacctc ataggcggcg agcggctgcc ggtagaatcc ggcgaaggtc tcgagacgct
     1141 ccgtggcgat cggttgcgtc cgcggcgcgt ccagcgcgag accgagctcc tcccggagca
     1201 cgtggcgctc gatctcgtga ttcggcaggc tgccccgcgc gctgttggtg agaatcgcga
     1261 aggcgaagtg gcgggacggg acgatcgtca gctgcgtctg gaagccgctg tatgagccac
     1321 cgtgcccgat caccttcgtg ccgtcggtcg tatagcggat cgtccagccg atcccccagc
     1381 tctcgttgtc ggcatcgcgc aggcggatgt atggctcctg catcgagcgg cggcgctcgt
     1441 ccgacagccc gagcggtccg ccggtcccat cgatgtgcca ctgcgcaaaa gtcaggagat
     1501 cgacgacggt cgagatgacg ccacccgatg gcgtgcgggc gcgaggatag gcgtaggagc
     1561 ggatcaggcg atgcgcgcca tcggtggctg actttggctg gtgcccgagt gcgaccgggt
     1621 agacgatcgc ttcctcggcg gtgaagacgg agcgctcgag gccgagcggc ttgtagacac
     1681 gctcgcgcat cgcctcctcg aaggtcgtgc cgagcagcgt cgcgatcacg tgtccggcga
     1741 gccagaagcc ggtattgccg taggacatga tctgtcccgg ctccgcccac tggtgcagcc
     1801 cgccgtagaa accgatcgcc tcgcggagcg cgtcctcgcc attgccgacg gtcgccagat
     1861 cgaccgcgag ttcgcaatcg atgccggcgg tgtggctgag caggtggcgc atcgtgatcc
     1921 gtgcctggcg ctcgggatcg ccgtcggtga gggtgacgcc cggcagatag gcgccgatcg
     1981 ggcgatcgag atcgaggagg ccgtcgttga cgagggtcat cgcgagcgtc gccgtgaacg
     2041 gcttcgtgat cgaggcgacg cgaaagagcg tctccggggt gaccggctgt tgcgtctcga
     2101 ggctggtgac gccgaaggag gcgaagctgg tctggccatc cttgaggatg ccgatcgcgg
     2161 cgccagggac gttccagcga tcgagcgatt cctgggcggc tgatgcgatt tcaggggcgg
     2221 tcatggggag cctttcgcgg gggacggatc gaactccccg cacgatacga gcttttcccg
     2281 aaccgcgaaa gtggcgctcc gcccaaatcg cgtaagccca gatgcgaagg agcgccgggc
     2341 gattgcccag cgctccttct gtttccgagg gtgtacgtga gtgttacggc agctggccgt
     2401 cgaggtcgat cttccagacc ccgtcgacga gttggaagct catctcacca tcgggattga
     2461 aggtgtaggt gccatcgccg ttatcgatga ctttgacgat gtcgacgaag acgtatgtac
     2521 cgtcggctga tgcgttgaca gagccgttga catctatcgc tcgaggagac atccccgcga
     2581 cggatcccgg ggacagcgcc agctgaccga actcctcgtc ggcttcctcg aggcgatcaa
     2641 ggagctcgtt gatcgtcggt tctgtgtatg cggtcatcgt tcgcgcgtcg gagtaaatgg
     2701 cttcgcgaat gtactggtct gtctccagcg ccatctgctg gtacgtgctg cccagggcct
     2761 tgcacacctg ccactgccga tacgcggctt gtatgtcgtc aaccggga
//
LOCUS       DASTMD010000241         1719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_380022, whole genome shotgun sequence.
ACCESSION   DASTMD010000241 DASTMD010000000
VERSION     DASTMD010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380022"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..759
                     /locus_tag="VFQ54_03625"
     CDS             262..759
                     /locus_tag="VFQ54_03625"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0164107.1"
                     /translation="MDLLDRLLDHDRWTTARVLDLSRGLSDAELDRRFDIGHATLRAT
                     YEHMIFSLEVWTSGMVGQRLMSESDDLSVATLFERHERIYPIFADIARRMQDEQRLDE
                     TFVDPWIDSPVTLRTFGTGTAHVLLHDMQHRSETLHILKRLGVTDLPDGDPKEWEEAN
                     QIVQG"
     gene            899..>1719
                     /locus_tag="VFQ54_03630"
     CDS             899..>1719
                     /locus_tag="VFQ54_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164108.1"
                     /translation="MASSTSATPPTADARNSPGPAWTRGWGIAIAGAIVALILEAIFI
                     GSYVGALHDPKPHDALPVGIVAPAQTASQLVQQIDAQAAGIIDPRVETDETALRHAID
                     ERKIYGGLVFGPRGTTLIVADAAGSSAVTVLTTFAQGLATGQKIPLTVEHVHLLDSGD
                     PRGLTTIYLVLAWVFGGYFCATVLTTLRGTGYASRGHAALRLSLLAGYGVLSGIVGAL
                     LAGPVIGAIDGHFLAIALSGTLIVFAVAATTMALQLVLGAAGTLLVMIAFVLLGNP"
BASE COUNT          304 a          596 c          513 g          306 t
ORIGIN      
        1 cgggatgatc ctcgtgacgg cggtcgtggt ggttcttctc gcggaaccac gccattacca
       61 gtcttcaggg acctcgcacg gccgcgtctt gaccggcgcg atcctgatac cggaacagcg
      121 acggtccgca gagcgtcaca cccatttccc tgcagttgtg accggaaccg ttccattaac
      181 aggacaggcg atgtccgcat acgcatccat gatgcaggta tctcgatgct gcgagatgcg
      241 cgttcgcaaa ggaggtaacg gatggatcta ctcgatcgcc tgttggatca cgatcgctgg
      301 acgacggcac gtgtgctgga cctgagtcgc ggactgagcg acgcggaact cgatcgacgg
      361 ttcgacatcg gacacgcgac gctccgcgcg acgtacgagc acatgatctt cagcctcgag
      421 gtctggacct cggggatggt cggacagcga ctcatgtcgg agtcagatga tctatcagtg
      481 gccacgctgt tcgaacgcca cgagcgaatc tacccaatct tcgcggacat cgcacgccgg
      541 atgcaggacg agcaacgtct ggatgagacc ttcgtcgacc cctggatcga ctcgcccgtc
      601 acgctcagga cattcggaac gggaaccgcg cacgttctcc tgcacgacat gcaacaccgg
      661 agcgagacgc tccatatcct caaacgcctc ggcgttaccg accttcccga cggcgatccc
      721 aaggaatggg aagaagcaaa ccagatcgtc cagggctgaa gatcgggctg ctcccaggat
      781 cacctacaga agcaagaatt gcccgtgata tcaggggtgc catacaatcg cagtgcgaga
      841 tcgccgtgcg cggctggctt gaccgcgcca ttaccgcccc ggcttttgag gagatcccat
      901 ggcatcctcc acgtccgcca ccccgcccac cgctgacgcg cggaattctc ctggcccggc
      961 ctggacccga ggttggggga tcgccatcgc cggcgcgatc gtggccctga tcctcgaggc
     1021 gatcttcatc ggctcctacg tcggcgccct gcacgatccg aagccgcacg atgccctccc
     1081 cgtcggcatc gtggcgcctg cccagactgc cagccagctc gtgcagcaga tcgacgccca
     1141 ggccgccggg atcatcgatc cccgcgtcga aaccgacgaa accgctctgc gtcacgccat
     1201 cgacgagcga aagatctacg gcggtctcgt cttcgggccg cggggaacca ccctgatcgt
     1261 cgccgacgcc gcgggttcct ccgccgtcac cgtcctcacc accttcgccc agggactcgc
     1321 cactggccag aagatcccac tgaccgtcga gcacgtccac ctgctggaca gcggcgaccc
     1381 gcgcggcctc accaccatct atctcgtgct ggcatgggtc ttcggcggct acttttgcgc
     1441 cacggtactg acgacgctcc gcggcaccgg ctacgcgagc cgcgggcacg ccgccctgcg
     1501 cctctccctg ctggcaggat acggcgtgct ctcgggaatc gtcggcgcgc tcctcgccgg
     1561 tccggtgatc ggtgcgatcg acgggcactt cctggcaatc gcgctttcgg gcacgctcat
     1621 cgtcttcgcg gtcgcggcga cgacgatggc gcttcagctg gtgcttggcg ccgctggcac
     1681 gttgctcgtg atgatcgcct tcgtgctgct cgggaatcc
//
LOCUS       DASTMD010000242         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1986796, whole genome shotgun sequence.
ACCESSION   DASTMD010000242 DASTMD010000000
VERSION     DASTMD010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986796"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFQ54_03635"
     CDS             <1..408
                     /locus_tag="VFQ54_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0164109.1"
                     /translation="GTFQVTQLVLPQMLERGDGRIINISSQLAYKGRAGWAHYSAAKA
                     GIIAFTRVLAQEVSRQGVLVNCIAPGPIETDIVPKGTVTDPEQSARILDAIPLGRSGT
                     VEEVAPTAVFLASDDATYYVGQTLGPNGGDVML"
     gene            470..1240
                     /locus_tag="VFQ54_03640"
     CDS             470..1240
                     /locus_tag="VFQ54_03640"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0164110.1"
                     /translation="MDNREFTEKRVVITGAGSGIGRATALAFATQGASLILLDRSQTG
                     LDETVAQLPEGSTAATILCDVSRVSEIEAAFGEIEAEHGEIDILVNNAGINPAVPTSL
                     DIDESFYDEVMDVNAKGIFFCAREALRSMIASEKGVIVNLGSVSGMIGWGGSSIYSAS
                     KGAVIALTKALAMEVAPHGIRVNAICPGTVRTPMVANVIDARPDPEAGWAASANFHPL
                     GRVAEPSDIANGILYLASDRSSFVTGTTLVVDGGLTAR"
     gene            1258..2025
                     /locus_tag="VFQ54_03645"
     CDS             1258..2025
                     /locus_tag="VFQ54_03645"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0164111.1"
                     /translation="MIIDSHTHVDDFPGATWSDPPEMIVGLLDEAEIDRAVIMTYRDA
                     TGQGEDIIEYIAEAVRAYPDRLIGYARMNPRDGDAALDLFDRAIREYNLRGLKLHPVS
                     YVMHPMTPQTIALIEHAARLGVPTLFHCGDEEFTLPLQIADVADAVPQAEIILGHMGG
                     YFHVKDAIRVAKRHPNLILETSAMPYPAFVKQAVDEIGADRILFASDGPGCDPTIEVE
                     KIRRAGLTPEDEEKILFGNFNRLLKLDAPAAPTTPAN"
BASE COUNT          360 a          723 c          624 g          343 t
ORIGIN      
        1 ggcacgttcc aggtcaccca gctcgtcctg ccccagatgc tcgagcgcgg cgacgggcgg
       61 atcatcaata tctcctcgca gctcgcctac aaggggcgcg ccgggtgggc gcactactcg
      121 gcggcaaaag ccgggatcat cgccttcacc cgcgtcctcg cccaggaagt cagccgccag
      181 ggcgtgctcg tgaactgcat cgcgccgggt ccgatcgaga ccgacatcgt gccgaagggc
      241 accgtcaccg atcccgagca gtcggcgcgc attctcgacg cgatcccgct cgggcgctcc
      301 ggcaccgtcg aggaagttgc gccgactgcc gtcttcctcg cctccgatga cgccacgtat
      361 tatgtggggc aaacgctggg tcccaacggc ggggacgtca tgctctgatg ccgccgggag
      421 ccgccaccct gaatcacgac cactcgactg gaaaggaccc tgcgcgttca tggacaaccg
      481 tgagttcacg gaaaaacgtg tcgtcatcac cggcgccggt tcggggatcg ggcgcgccac
      541 cgcgctcgcc ttcgccaccc agggcgcatc cctgatcctg ctcgatcgct cccagaccgg
      601 tctcgacgaa acggtcgcgc agcttccgga aggctcgacc gccgcgacga ttctctgcga
      661 cgtctcgcgc gtctccgaga tcgaggccgc attcggggag atcgaggcgg aacatggcga
      721 gatcgacatc ctcgtcaaca acgccggcat caatccggcg gtccccacct cgctcgatat
      781 cgacgagtcg ttctacgacg aggtgatgga cgtcaatgcc aaggggatct tcttctgcgc
      841 ccgtgaggcg ctccgctcga tgatcgcgag cgagaagggc gtcatcgtca atctcggttc
      901 ggtgtcaggg atgatcggct ggggtggaag cagcatctac tcggcgtcga agggcgccgt
      961 catcgccttg accaaggcgc tcgcgatgga ggtcgcgccg cacggaatcc gcgtcaacgc
     1021 gatctgcccc ggcaccgttc gcacgccgat ggtcgcgaac gtgatcgatg cgcgacccga
     1081 tccggaggcg ggatgggctg ccagcgcgaa cttccatccc ctcggtcgcg tggccgagcc
     1141 gtccgacatt gccaacggca tcctctacct cgcttccgat cgcagttcat tcgtcaccgg
     1201 caccacgctc gtcgtcgatg gcggcctgac cgcccgctag attctcagga gagacgcatg
     1261 atcatcgatt cgcacaccca cgtcgacgac tttcccgggg ccacctggtc cgatcctccc
     1321 gagatgatcg tcggcctgct cgacgaggcc gagatcgatc gcgccgtcat catgacctac
     1381 cgcgacgcga ccggtcaggg cgaggacatc atcgagtaca tcgcggaggc ggtccgcgcc
     1441 taccccgatc gcctgatcgg gtatgcacgg atgaacccgc gggacggtga cgccgcgctc
     1501 gatctcttcg atcgcgcgat ccgcgagtac aacctgcgcg gcctcaagct ccatccggtc
     1561 agctacgtga tgcacccgat gacgccgcag acgatcgcgc tgatcgagca cgcggcgcgc
     1621 ctcggcgtgc cgacgctctt ccattgcggc gacgaggagt tcacgctccc gctgcagatc
     1681 gccgatgtcg cggatgccgt gccccaggcc gagatcatcc tcggccacat gggcggctac
     1741 ttccacgtca aggacgcgat ccgcgtcgcg aagcgtcatc cgaacctgat cctcgagacc
     1801 tcggctatgc cctaccctgc cttcgtgaag caggcggtcg acgagatcgg cgccgatcgc
     1861 atcctcttcg caagtgatgg tcctggctgc gatccgacga tcgaggtcga gaagatccgc
     1921 cgcgccggcc tcaccccgga ggacgaggag aagatcctct tcggcaactt caaccgcctg
     1981 ctcaagctcg atgcgcccgc tgccccgacg actccggcca actgaaaggt cccaccacga
     2041 tgcttatcga
//
LOCUS       DASTMD010000243         1568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1361434, whole genome shotgun sequence.
ACCESSION   DASTMD010000243 DASTMD010000000
VERSION     DASTMD010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361434"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFQ54_03650"
     CDS             complement(<1..557)
                     /locus_tag="VFQ54_03650"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEU0164112.1"
                     /translation="MASVLVGHRAVAAKQIAVGGAAVFPVEPDQLLQVVDLYGKQVAS
                     LVAFTGEHNEDRLSTPVTITMNSSIVLKMGDRLYSAQGVALFEIVDDTVGRHDLLTAP
                     LAIDMDLTSTVKSKVSTLDSLKQAATEAGLETADVSDPVNLFKHVVIRQKGELDVRES
                     FSERNDAVVLRALAPGRVIVANAYS"
     gene            778..>1568
                     /gene="chrA"
                     /locus_tag="VFQ54_03655"
     CDS             778..>1568
                     /gene="chrA"
                     /locus_tag="VFQ54_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEU0164113.1"
                     /translation="MNPRLKAVVLLALQLGFTAFGGPAAHIAMLREQAVVRRKWLTEQ
                     EYLDLLGVTNLIPGPNSTEMVMHVGYRRGGWPGLVLAGSAFILPAASITLVFAWLYVE
                     AGTTTQGEWILKGIKPVVLAVVLQAVWNLGLTAVKDRRLAAVGVAVFGLYLLGINEIA
                     LLFGGALVAAGSAWIQRRFQSRERSLPTLGLAPLPLIPALLQMADPAVSYSAWRLFLG
                     FLKIGALLYGSGYVLVAFLQSEFVDRLRWITEQQLLDAVAVGQFTP"
BASE COUNT          265 a          513 c          479 g          311 t
ORIGIN      
        1 tcgctgtagg cgttcgcaac aatgacgcga cccggcgcca gcgcgcggag cacgacggca
       61 tcgttccgct ccgaaaacga ttcccgcacg tcaagctcac ccttctgccg gatgacgaca
      121 tgcttgaaga ggttcacggg atcgctgaca tcggcggtct cgagaccggc ctcggtcgcg
      181 gcctgcttca ggctgtccag cgtcgagacc ttcgacttca ccgtgctcgt gagatccatg
      241 tcgatcgcca gcggcgccgt caggagatcg tggcgaccga cggtgtcgtc gacgatctcg
      301 aagagcgcca cgccctgcgc cgaatagagg cggtcaccca tcttgagcac gatcgacgag
      361 ttcatcgtga tcgtgaccgg cgtggagagg cgatcctcgt tgtgttcacc ggtgaaggcg
      421 accagactcg cgacctgctt gccgtagaga tccactacct gaagcagctg atccggttcg
      481 accgggaaca ccgccgcgcc gcccaccgcg atctgcttcg ccgcgaccgc gcgatgcccg
      541 accagtacgc ttgccatgac tatcttgctt cctgcttcgc gagagaatga tgagtacgca
      601 cccatgccac accacgctcg ttgagcgtta ttctcgcagg gaagatgcat cccggtccaa
      661 tgctccgcca acctgactaa attcctgtga acgccgctcg tcctgctcga caccaccgga
      721 gtgggaatgt catggtggtt ggatgagatg ccacgacgag aggatgacgc acgcttcatg
      781 aatccccgat tgaaagccgt ggttctcctg gcactccagc tcggattcac tgccttcggg
      841 ggaccggcgg cgcacatcgc gatgttgcgc gaacaggcgg tcgtccgccg caagtggctc
      901 accgaacagg agtatctcga cctgctcggc gtcaccaatc tcatcccggg cccgaactcc
      961 acggaaatgg tgatgcatgt cggatacagg cgcggcggtt ggccggggct tgttctggcc
     1021 ggcagcgcct tcatcctgcc ggcggcgtcg atcacgctcg tttttgcctg gctctacgtc
     1081 gaagcgggaa cgacgacgca gggagaatgg attctcaagg ggatcaagcc ggtcgtcctc
     1141 gccgtcgttc tccaggcagt ctggaatctt ggcctgaccg ccgtgaagga caggcgactg
     1201 gcagcggttg gtgtcgccgt cttcggactc tacctgctgg ggatcaatga gatcgcgttg
     1261 ctcttcggcg gcgccctcgt cgcggcgggg agcgcctgga tccagcggcg gttccagtcc
     1321 cgcgagcgga gcctgccgac gctgggtctc gccccgctcc cgttgatccc ggcgttgctc
     1381 cagatggcgg atcccgccgt ttcctactcg gcatggcggc tcttcctcgg attcctcaag
     1441 atcggcgccc ttctctatgg atcgggctac gtcctcgtcg cctttctcca gagcgaattc
     1501 gtggatcgcc ttcgctggat caccgagcaa caactgctcg atgccgtcgc ggtcgggcag
     1561 ttcacgcc
//
LOCUS       DASTMD010000244         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1630899, whole genome shotgun sequence.
ACCESSION   DASTMD010000244 DASTMD010000000
VERSION     DASTMD010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630899"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFQ54_03660"
     CDS             complement(<1..435)
                     /locus_tag="VFQ54_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HEU0164114.1"
                     /translation="MGDSADETLKRTALYDRHVAAGARLIPFAGWEMPVQYEGILAEH
                     RRVRSQAGLFDLGHMGQVDITGPDALQFLQYTTTNDIAKLQPGQANYGMLPNERGGVV
                     DDIIVYRKPAGEEGFMVVINASNMERDVEWWNELRTRRSDFDV"
     gene            complement(535..1992)
                     /gene="purF"
                     /locus_tag="VFQ54_03665"
     CDS             complement(535..1992)
                     /gene="purF"
                     /locus_tag="VFQ54_03665"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU0164115.1"
                     /translation="MKTGSAIQTDTVNLEDFFSDYEDKPHEECGVFGIYAPGEDVARL
                     TFFGLYALQHRGQESAGIATADGHDVAVHTSLGLVSNAFNEEDLRHLPGHIAIGHTRY
                     STSGSNQACNAGPIRVESDLGTIVFAHNGNVTNALALHRELEAEGVEFESTTDSEVLA
                     KLVARTPGITFVEKMRRAMPKLIGAYSLAVLVDDRMYAVRDPKGVRPLCIGKLAGHYV
                     VASETCALMTIGAEFEREVEPGEIIEFNDRGMISHGIGQAQDQALCLFEMIYFARPDS
                     EIKGRRLYLSRQRMGVELAREAPVDADVVVSMPDSATPAAIGYAREAGIPYAEALIKN
                     RYIGRTFIQPDQRLREIGVSLKFNVLPEVVEGKRVVLVDDTIVRGTTSRPIVQLLKQA
                     GAKEVHMRVHAPPMMWPCYLGVDLAKRDELIAARMTVPEIGEFIGADSIGYLSLEGLF
                     RAIGRGSTGFCTGCLTGNYPVPVFANSDKLALEAV"
     gene            complement(2023..>3428)
                     /locus_tag="VFQ54_03670"
     CDS             complement(2023..>3428)
                     /locus_tag="VFQ54_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEU0164116.1"
                     /translation="VECASRGGVGVDIDVAFVPRRETGMTAYEIMLSESQERMVLVTP
                     PDRVDEITEVLNRWSLSSAVIGRITDDGIVRVRDGEKVEVEVPVTFFIDECPVYEPVN
                     AFEPQDFVDRRTKDLSSLADLERDAVVPTIEKLLASPNIGSRRGIWEQYDHTILTNTV
                     TGPGRGDAAVLRVKGTDTAIAISLDCNSRYCYLDPFEGARRAVAESTRNVSCVGGTPL
                     AITNCLNFGNPERVPGNWQLMRAVEGMADACNALGIPVVSGNVSLYNETAGRPVFPTP
                     TVGCVGVLPEVTHTVGLGWKEGDTVVLLGGTNPSLAGSEYLSWIGGDVAGSLAPFDAA
                     AELAVQTLVRSLVSDSVVNAAHDIGLGGLALALVEMALANNVGATIELPGIVASSRLD
                     ETWFGESASRILVSVETARVDDVIGRAADAGVDAYVLGATGGDAISLAGFGSLALHAL
                     LETSESALRSDGIVANA"
BASE COUNT          620 a         1112 c         1080 g          616 t
ORIGIN      
        1 cacatcgaaa tccgaacggc gcgtgcgcag ctcgttccac cactcgacgt cgcgctccat
       61 gttggacgcg ttgatcacga ccatgaagcc ctcttcgccc gccggcttgc ggtagacgat
      121 gatgtcgtcc acgacgccgc cacgttcatt cggcagcatg ccatagttgg cctggccggg
      181 ctgaagcttg gcgatgtcat tcgtggtcgt gtactggaga aactggaggg catccggccc
      241 ggtgatatcg acctggccca tatgtccgag gtcgaagagc cccgcctgcg agcggacgcg
      301 ccgatgctcg gcgagaatgc cctcgtattg aacgggcatt tcccaccccg cgaacggaat
      361 cagtcgtgct cccgcggcga catgtcggtc gtagagcgcc gtgcgcttca atgtctcatc
      421 ggcagaatcg cccaccggac ctcctccagt ttgaaaccgc gcatgcgcgc gccaaacagc
      481 cacttcaccc tattccgatg aagatacccc gagaatcatg agttgcgcta tggatcagac
      541 cgcctcgagc gcgagtttgt cactgttcgc gaagacggga accgggtaat ttcccgtcag
      601 gcagccggtg cagaagccgg tcgagccgcg accaatcgcc cggaagagcc cttcgagtga
      661 aaggtagccg atgctgtctg ccccgatgaa ctcgccgatc tcggggaccg tcatccgcgc
      721 ggcgatcagc tcatcgcgct tcgcgagatc cacaccgagg tagcacggcc acatcatcgg
      781 cggcgcatgc acccgcatgt gcacttcctt cgctccggcc tgcttgagca gctgtacgat
      841 cgggcgactg gtcgtacctc gcacgatcgt gtcatcgacg agcaccaccc gcttgccttc
      901 gacgacttcc ggcaacacgt tgaacttcag cgaaacgccg atctcgcgaa gccgctggtc
      961 tggctggatg aaggtgcgtc cgatgtagcg gttcttgatc agcgcctcgg cgtatggaat
     1021 tcccgcctcg cgggcgtagc cgatcgctgc cggtgtcgcc gaatccggca tgctaacgac
     1081 gacatcggca tccaccggcg cttcgcgcgc gagctcgacg cccatccgct ggcgactgag
     1141 gtagagccgg cgacccttga tctcgctgtc cgggcgcgcg aagtagatca tctcgaagag
     1201 gcagagcgcc tggtcctgcg cctggccgat gccgtgggag atcatgccac ggtcattgaa
     1261 ctcgatgatc tcgcccggct cgacctcacg ctcgaactcc gcgccgatcg tcatcagggc
     1321 gcaggtctcg gaggcgacga cgtaatgacc ggcaagcttc ccgatgcaca gcggacgcac
     1381 gcccttcggg tcgcgcaccg cgtacatccg gtcgtccacg aggacggcga ggctgtaggc
     1441 gccgatcagc tttggcatcg cgcgtcgcat cttctcgacg aaggtgatgc ctggcgtgcg
     1501 tgcgacgagc ttcgcaagga cctcgctgtc ggtcgtcgat tcgaactcga caccctcggc
     1561 ctccagctcg cgatggagcg cgagtgcgtt cgtcacatta ccgttgtggg cgaagacgat
     1621 cgtcccgagg tccgattcga cccggatcgg acccgcgttg cacgcctggt tcgaacccga
     1681 tgtcgagtac cgcgtgtgcc cgatcgcgat gtgacctggc aggtgcctga gatcttcctc
     1741 gttgaacgca ttcgaaacga gcccgaggct cgtgtgaacg gcaacgtcgt ggccgtccgc
     1801 ggtcgcgatg ccggcgctct cctggccgcg atgctgcaag gcatagaggc cgaagaaggt
     1861 caggcgggca acgtcctcgc ccggcgcata gatcccgaag acgccgcact cctcatgcgg
     1921 cttgtcctcg taatcactga aaaagtcctc caggttgaca gtgtcggttt ggatggcgga
     1981 cccggtcttc atcctgtttt ctccacgaag aggcatgcgg cactaggcgt tggcgacgat
     2041 cccatcggat cgcagcgcgg attcgctcgt ttcgagcaag gcgtgaagag cgagcgagcc
     2101 aaacccggcg agggagatcg cgtccccacc ggtcgcaccg agcacatatg cgtcgacccc
     2161 ggcatcggcg gcgcgaccga tcacgtcatc gacgcgggcg gtttccacgc tgacgagaat
     2221 ccggcttgcc gattctccga accaggtctc gtccagtcgt gatgacgcca cgatcccggg
     2281 aagttcgatg gtggcgccga cgttgttggc caacgccatc tcgacgagcg cgagggcgag
     2341 tccaccgaga ccgatgtcat gtgccgcgtt taccacggaa tcggagacga gtgacctcac
     2401 cagggtctga acggccagtt cggcggcggc atcgaacggc gccagcgacc cggcgacgtc
     2461 gccgccgatc catgaaaggt attcgctccc ggcgagtgaa ggattcgtgc cgccaagcag
     2521 gacaacggtg tcgccttcct tccagccaag cccgacggta tgcgtgactt ccgggaggac
     2581 gccgacgcag ccgaccgttg gcgtcgggaa gacgggtcga ccggcagttt cgttgtagag
     2641 gctgacgttt ccgctcacga ccgggattcc cagggcgttg caggcgtcgg ccatgccctc
     2701 aacggcccgc atcagctgcc agtttccggg aacgcgctcg gggttgccga agttgaggca
     2761 gttcgtgatc gcgagcggag tgccaccgac gcagctgacg ttccgggtcg actccgccac
     2821 ggcacggcgc gcgccttcga aggggtcgag gtagcagtaa cgggaattgc agtcgaggga
     2881 gatcgcgatc gcggtatcgg tacccttcac gcgaagaacc gcggcatcgc cgcggcccgg
     2941 tcccgtcacg gtgttcgtga ggatcgtgtg atcgtactgc tcccagatcc cacgccggga
     3001 tccgatgttg ggagaggcga gaagcttctc gatggtcggg acgacggcat cgcgctcgag
     3061 atcggcaagt gaactcaggt ccttcgtccg ccgatcgacg aagtcctgcg gctcgaacgc
     3121 gttgactggc tcatagaccg ggcactcgtc aatgaagaag gtcaccggca cttcgacctc
     3181 gaccttctcg ccatcgcgca cgcggacgat gccgtcatcc gtgatccgtc cgatcaccgc
     3241 ggacgagagc gaccaccggt tcagaacctc ggtgatctcg tcgacacgat ctggcggcgt
     3301 gacgaggacc atgcgctcct ggctctcgct gagcatgatc tcgtaggcgg tcatcccggt
     3361 ctcgcggcgg ggaacgaagg cgacgtcgat atcgacgccg acgccgcctc gactcgcaca
     3421 ttcgacga
//
LOCUS       DASTMD010000245         3261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2255234, whole genome shotgun sequence.
ACCESSION   DASTMD010000245 DASTMD010000000
VERSION     DASTMD010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2255234"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFQ54_03675"
     CDS             <1..329
                     /locus_tag="VFQ54_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEU0164117.1"
                     /translation="SIRIHQREKLARILELMGHKKEDQADRFGAILDAFEYGAPPHGG
                     IAMGIDRFTMILADEASIRDVIAFPKNQRGYDLMFQAPSPVDTIQLDELGLALKPQPV
                     AADAGE"
     gene            complement(846..1820)
                     /locus_tag="VFQ54_03680"
     CDS             complement(846..1820)
                     /locus_tag="VFQ54_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164118.1"
                     /translation="MTRRKIPALITTALCLLVSLTTLMVAPAANAQSDSPGGHILYIR
                     SGDIWEWSPDHDDMLLKDGSATSPAWSPDESEVLYTRNGGSYSNLVLYRPTTGTRSAL
                     TNNESYDEKGSQDYVLGSSWANDPYWSASGIICFVSDVQSQNGEMALWVLASTDAQPY
                     LAASDGSDAGNIEHVSVDAAGTWAVYTVEAPDDATGGITYVALRNLNTGATSRIAEGA
                     TGAYDADISQDGTKVVMSIRNASGTSDLWLYDITSGNLTQLTAGQDASRARWSPDDTW
                     IAYIKPGDNTFETWAIPINANRDGASGDAVKLFSHDDIDAPGGLSWGS"
     gene            complement(1861..>3261)
                     /locus_tag="VFQ54_03685"
     CDS             complement(1861..>3261)
                     /locus_tag="VFQ54_03685"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU0164119.1"
                     /translation="GGLFAALTGAELDGHRYADAAVANGAVAILSERPLPLPIPQIVV
                     PDSRAALAILANAFFGYPSHELAAVIGITGTDGKTTTSYILENILRHAGRSVGVIGTV
                     GIRIGNGVSYDLGHQTTPESHLNQSYLREMADAGIEHAIVESTSHGLAMHRLDGTAFR
                     IAGVTNVTHEHLEYHKTIENYRRAKAILIERVAENHGVVVLNADDPGASSMSAWAAGA
                     DVVHYGIHRDDSHLSAHNVVARNDGSDFQLRIGIERNHLHLPLVGEFNVANALLAAGL
                     ANALGIAATTIAEALSKPVAVPGRMHRIDAGQPFNVVVDYAHTPESLTKILTLLRGLH
                     PGSRLIAVFGSAGDRDVQKRPMQGRVAAELADYAIFTNEDPRREDAEKILRDIASGAI
                     ERDGVEGRTFEIVVDRRAAIALAFERAQPGDCVLLAGKGHEQSISWNGIELPWDETTV
                     AEELLAERGYTSRILS"
BASE COUNT          535 a         1036 c         1085 g          605 t
ORIGIN      
        1 gcagcatccg aatccaccag cgcgagaagc tggcgcggat cctcgagctg atgggacaca
       61 agaaggaaga ccaggcggac cgcttcggcg cgatcctcga tgccttcgag tacggtgcgc
      121 cgccgcacgg cgggatcgcg atggggatcg accgcttcac gatgatcctg gccgacgagg
      181 cgagcatccg cgacgtgatc gccttcccga agaaccagcg cggctatgat ctgatgttcc
      241 aggcgccctc gccggtcgac accattcaac tggatgagct cggcctcgcc ttgaagccgc
      301 agcccgttgc cgccgacgcg ggcgagtagc gcctcgccct acgaacgacc gctggagccg
      361 gggatcgtcg cctggcggtc gccatgccct ctacggtgtg agaacgaccg gggtctgtag
      421 ggcgagagtt tatctccaac gctgtcgcct gcccattcgt catttcgagc ctgtcgagag
      481 atcccacggt tcgtaggggc gagcctggtg ctcgcccgcc gccccgttcg acgagctcag
      541 ggcgggctcc ggcggaccgc ggcggaatct cccacgcgcg acgcgctccc ggaggcggat
      601 cggcgcagcc gcattgatcc gaggcgatta cccgggtcgc cgggacggga accgagcgcg
      661 gcacagggag ggtgggacgg aacctccctg tctcgcccct cggcggcgct cagggcacgc
      721 tccgaagtct caccctacgg gcgacctgcg tacgtccagc gtccgttcgt agggcgggac
      781 ttctatgtcc cgccttctgg agctccaaac gcggacacga cgtgatgcga tcgtccccgg
      841 cgatcctagc tgccccagct gaggccgccg ggagcatcga tgtcgtcgtg ggagaagagc
      901 ttcacggcgt ccccggatgc gccgtcgcgg ttcgcgttga tcgggatcgc ccaggtctcg
      961 aaggtgttgt cgcccggctt gatgtaggcg atccaggtgt cgtccggcga ccagcgcgcc
     1021 cgggacgcgt cctgtcccgc cgtaagctgc gtgaggttgc cgctggtgat gtcgtaaagc
     1081 cagagatccg atgtgcccga ggcgttgcgg atcgacatca cgaccttcgt gccgtcctgc
     1141 gagatgtccg cgtcataggc accggttgct ccctccgcga tgcggctcgt cgctcccgtg
     1201 ttcaggttcc tgagcgcgac gtaggtgatg ccgccggtgg cgtcgtccgg cgcctccacg
     1261 gtatagacgg cccatgtccc ggcggcgtcg accgagacgt gctcgatatt gccggcatcg
     1321 ctgccgtcgg atgccgcgag atacggctgc gcgtccgtcg aggcgaggac ccagagcgcc
     1381 atctcgccgt tctgcgactg cacgtcggag acgaagcaga tgatgccgga ggcgctccag
     1441 tacggatcgt tcgcccagct ggagccgagg acgtagtcct ggctgccttt ctcgtcgtac
     1501 gactcgttgt tcgtgagggc gctgcgggta ccagtcgtcg ggcgatagag aacgaggttg
     1561 gagtagctgc cgccattccg ggtgtagagc acctcggact cgtcgggtga ccatgccggg
     1621 gacgtcgcgg agccgtcctt gagcagcatg tcgtcatgat cgggcgacca ctcccagatg
     1681 tcccccgatc ggatgtaaag gatgtgaccg cccggggaat cagactgcgc gttcgcggcc
     1741 ggcgcgacca tcaatgtcgt caggctgacg aggaggcaga gcgccgtcgt aatcaaggcg
     1801 ggaatcttcc ggcgcgtcac gatccgaggc cttgtcatcg tttcctgcct tacgggggcg
     1861 ctaactgagg atgcgactgg tatatccgcg ttcggcgaga agctcttccg cgaccgtcgt
     1921 ctcgtcccac ggaagctcga tcccgttcca gctgatgctc tgctcgtgac ccttgcccgc
     1981 gagcaggacg cagtcgccag gttgcgcgcg ttcgaaggca agggcgatcg ccgctcgccg
     2041 gtcgacgacg atctcgaatg tccgaccctc gacaccgtca cgttcgatcg ccccggaggc
     2101 gatatctctc aggatctttt ccgcgtcttc gcgacgagga tcttcgttag tgaagatcgc
     2161 gtaatccgcc aactcggcgg cgacgcgtcc ctgcatcggt cgtttctgga cgtcacgatc
     2221 gccggcgcta ccgaagacgg cgatcagtcg actcccggga tgaagcccgc ggagcagcgt
     2281 caggatcttc gtcagcgatt ccggagtatg ggcgtagtcc acgacgacat tgaatggctg
     2341 ccctgcgtcg attcgatgca tccgacccgg gaccgccacc ggtttgctca atgcttcggc
     2401 aattgtcgtt gccgcaatcc cgagcgcatt ggcgaggcct gccgcgagta gcgcattcgc
     2461 gacgttgaac tcacctacga gtggcaggtg gaggtggttt cgctcgatgc cgatccgaag
     2521 ctggaagtca ctgccatcgt tgcgcgcaac gacattatgt gcagacagat gcgagtcgtc
     2581 gcgatggatg ccgtagtgaa cgacatcagc accggccgcc cacgcactca tcgaacttgc
     2641 cccgggatcg tcggcgttga ggacgacgac gccgtgattc tccgccactc gttcgatcag
     2701 gatcgctttc gcgcggcgat agttctcgat cgtcttgtgg tattcgaggt gctcgtgcgt
     2761 cacgttcgtt acacctgcaa tgcggaaggc cgtgccgtcg agccgatgca tggcgagacc
     2821 atgcgaggtg gattcgacga ttgcgtgctc gatcccggca tcggccatct ccctcaggta
     2881 gctctggttg aggtgggatt ccggtgtcgt ctgatgtccg agatcgtagc tgacaccgtt
     2941 gccgatccga atgccgacgg taccgatcac gccgaccgat cgccccgcgt ggcgcaggat
     3001 gttctcgaga atgtaactcg tggtggtctt gccgtcggtg ccggtgatgc cgatgaccgc
     3061 cgccagctcg tgactcgggt agccgaagaa ggcattggcg agaatcgcga gcgcggcgcg
     3121 gctatccgga acgacaatct gggggatggg aagcgggagc ggacgctcgc tgaggatggc
     3181 gaccgccccg ttcgcaaccg cagcgtcggc gtatcgatgc ccgtcgagct cggccccggt
     3241 cagggcggcg aagagcccac c
//
LOCUS       DASTMD010000246         5067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_291281, whole genome shotgun sequence.
ACCESSION   DASTMD010000246 DASTMD010000000
VERSION     DASTMD010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291281"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..679)
                     /locus_tag="VFQ54_03690"
     CDS             complement(53..679)
                     /locus_tag="VFQ54_03690"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU0164120.1"
                     /translation="MTRYWSRGDQVLLRFRRSMNLALPLTVIEDGPEWTLLYAAPGTP
                     MKRRAQPDGSPIPREMGYAERMALPYAIADTIWATNHALIVIQPGVAHDVRFFWSGEW
                     EFRGSYVNLIQPVMRVATGFDTADHVLDIAVDPDGTWRWKDEGEFTDAVERGRFSVEE
                     AADVRAEGERMIPAIEARKWPFDGSFENWRPDPAWPVPVMPDNWNEDE"
     gene            complement(676..1350)
                     /locus_tag="VFQ54_03695"
     CDS             complement(676..1350)
                     /locus_tag="VFQ54_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU0164121.1"
                     /translation="MTESKIVTRDTANAAPFRQPGEQVLLRFTRPGYVYFVVPQTVVR
                     DDDEVTMTFTAGGTPILKRVYPDGKEIPRDLSYVERSRLPVVLGQGVWHPYHTLSITP
                     AGAEYDVRYCWHVDDWSFRGWYVNLQSPLRRVPTGFDSDDSMLDLTVTADGAWAWKDE
                     TELAEGVLVGRFSADEAAQIRAVGESVIPSIASRAYPFDGSLVEWRPDPAWPIPGIPD
                     GWDVTP"
     gene            complement(1629..3335)
                     /locus_tag="VFQ54_03700"
     CDS             complement(1629..3335)
                     /locus_tag="VFQ54_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164122.1"
                     /translation="MTQDQNKSHIHNQDAITLPSITTSRRSIVKGAGIAAGIAVAAAA
                     GPRITFAQTPETSPAASPGASDTVVPSGIEGVPDVYLKTPAVSRSYDGTPGNGGTVHA
                     FTISYNPPPPPKGQNQYWQELEKRLGVSWEIDMTPQPNYGEKSAAYLAGGDLPDLFYI
                     NPGQNATQQYQAMAQGAFLDLTPYVTGDAIKPFKNLASFPSYMWDNLKFQGKIFGVPN
                     PSLRAGNLPYYRTDWATNVGAEPTNPDSIAKMLVAFSKNDPNKDGSNNTWGMGRFNSG
                     WLVWDNLIGCYGFKVPNNWRLNADGTLTNAIETDEYKALIGYLAQLFKDGGYHPDAAS
                     MTFSDDQNAFIAGQTGLHYEGLTSFFGVGNVGYKLTQTTPSATIAPIVPVADDGSPGV
                     TYNNTGFFGFVGIPAKIGKDEDRVQELLRILDYLASPFGSVEQVFLANGIEGVHWNFD
                     ANGNRIVNDKGRSERSDLVYLMGSVQVLYYPEDPKIGLQVQKDLKSAATLGIDDPTLV
                     LFSQTNVDNGPQLNQLGTDSISSIVTGRASVDSLSDVIDQWKSQGGDQIRNEFQEALQ
                     GS"
     gene            complement(3496..4464)
                     /locus_tag="VFQ54_03705"
     CDS             complement(3496..4464)
                     /locus_tag="VFQ54_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0164123.1"
                     /translation="MAVTQPDAEFTQAVTVVGKKPLWPSRRSDARPVWMEEPGPAMRI
                     AKFLVLAVIVITMLFPIVYVVSMSFSSAVDAQRGGLVLWPEHPTLDAYRAIFDGGVVT
                     RALQISVMLTIVGTAAQMLFTTTMAYGMSRTSVLGSKVVLYIVLGALILSPGMIPSYL
                     LVKELGMINSYSALIIPGLISAWNLIIVRNFFMNLPQELLDAAKVDGANDFQIFMQVV
                     LPLSKAVLAVVALFYGVVIWNNFFNAILYLNDSTKWPIQVVLRQYVLMGSNLTSANEF
                     KAGQAPPPAQTIKMAIVVAGTVPILIVYPFLQRYFTKGVLTGSIKG"
     gene            complement(4464..>5067)
                     /locus_tag="VFQ54_03710"
     CDS             complement(4464..>5067)
                     /locus_tag="VFQ54_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0164124.1"
                     /translation="LISERIKRTIQSIVYLPYFLSWVIVVALWTQVFGGAGVINQALS
                     DHGMQTFNIMSDPSWFKPVMILETMWKEVGWGTIIFLAALTKIDIQLYEAAAMDGANG
                     RRRLLDVTLPGIRGVVILLLILRMGSMFSTGFEQYFLQRQAYGAHTAEVIDTLVYTLG
                     FQSGQWSFAAAVGLTRSLVAALLILGANWLAKRFGEEGLL"
BASE COUNT          933 a         1615 c         1559 g          960 t
ORIGIN      
        1 gcgacgacat cgccgtgctt ccattcggcg tccgggaagt gggtgtgatt tgtcattcgt
       61 cctcattcca gttgtcaggc atcacgggaa ccggccatgc cgggtcgggc cgccagttct
      121 cgaacgatcc gtcgaacggc cattttcgtg cctcgatcgc cggaatcatc cgttccccct
      181 ctgccctgac atctgctgcc tcttcgacac tgaatcgtcc ccgctccacg gcatccgtga
      241 actcgccctc atccttccaa cgccaggtcc catccggatc gaccgcgata tccaggacgt
      301 ggtcggccgt atcgaatccg gtcgcaacgc gcatcaccgg ctggatcagg ttcacgtacg
      361 agccgcgaaa ctcccattct cccgaccaga agaaccggac gtcgtgcgcc actcccggtt
      421 gaatcacgat cagcgcatgg ttcgttgccc agatcgtgtc agcgatcgcg tatggaaggg
      481 ccattcgctc ggcgtatccc atttcgcgag gaatcggcga tccatcgggc tgcgctcgac
      541 gcttcatcgg cgttcccggt gccgcgtaca gcaaggtcca ctccggaccg tcctcaatca
      601 cggtcagcgg cagtgcgagg ttcatcgaac gacgaaagcg gaggaggacc tggtcgccac
      661 gactccaata gcgcgtcacg gcgtcacatc ccagccgtcc gggatgccgg gaatcggcca
      721 tgccgggtcg ggtcgccact cgacgagcga tccgtcaaag ggatacgcac gcgatgcgat
      781 cgacgggatc accgactccc cgacagcgcg gatctgcgcc gcctcgtccg ccgagaagcg
      841 cccgacgagg acaccctccg ccagttcggt ctcgtccttc catgcccacg cgccatcggc
      901 ggtaacggtc aggtcgagca tggagtcgtc gctgtcgaag ccggtcggaa cgcggcgaag
      961 gggagattgc aggttcacat accagccgcg aaacgaccag tcatcgacat gccagcaata
     1021 gcggacgtcg tattccgcgc cagccggcgt gatcgagagc gtgtgatagg gatgccagac
     1081 gccttgaccg agtacgaccg gcagcctcga ccgctcgacg tacgagagat cgcgtggaat
     1141 ctccttgccg tcgggataga cgcgtttgag gatcggcgtc ccgcccgccg tgaaggtcat
     1201 cgtgacctcg tcgtcatccc gcacgacggt ctgcggcacg acgaagtaga cgtaccccgg
     1261 ccgcgtgaac cggagcagca cctgctctcc cggctgacga aagggcgcgg cgttggcggt
     1321 atcccgggta acgatcttgg attcagtcat cggtggaaaa ctccgtggat cggctgtccg
     1381 agatcccacc gtaccacgtt cgtagaattc ttcggaaggg actccactgt tgcgcgcttc
     1441 tccaacgtgg cacagggcga ccgccaggat cgcccctacg atattaccca tcttcgtaga
     1501 gacatctctg acggtcgccc gatcctccga ttgcccgtgt cttgcccatg ctcgacgtgg
     1561 cgaggggccg gtgcgccatt gcaccggccc ctcaccacgc acgcattcat tacgaagacc
     1621 gcagggccct acgacccctg cagcgcctcc tggaactcgt tccggatctg atccccgccc
     1681 tgggatttcc actgatcgat cacatcggag agcgaatcaa ccgacgcacg acccgtcacg
     1741 atcgaggaga tcgagtcggt gccaagctgg ttcagctgcg ggccgttgtc gacattcgtc
     1801 tgcgagaaga ggacgagcgt cggatcgtcg atcccgagcg tcgccgcgct cttgaggtcc
     1861 ttctggacct ggagtccgat cttcggatcc tccggatagt agagaacctg caccgagccc
     1921 atcaggtaga caaggtcgct gcgctcggag cgacccttgt cgttcacgat gcggttgccg
     1981 ttcgcgtcga agttccagtg gaccccttcg atgccattag cgaggaagac ctgctccacg
     2041 ctgccgaacg gtgaggcgag gtagtcgagg attcgcagca gttcctgcac gcggtcctca
     2101 tccttgccga tcttcgccgg gatgccgacg aagccgaaga aaccggtgtt gttgtaggtc
     2161 acgccgggcg aaccgtcgtc cgccaccggc acgatcggcg cgatcgtggc gctcggtgtc
     2221 gtctgcgtca gcttgtaacc aacgttcccg accccgaaga acgacgtcag tccctcgtag
     2281 tggagccctg tctgaccggc gatgaaggca ttctggtcgt cgctgaaggt catcgacgcc
     2341 gcatccggat ggtagccgcc gtccttgaag agctgcgcga gatagccgat cagcgccttg
     2401 tactcgtcgg tctcgatcgc gttcgtcagc gtgccgtccg cattcagacg ccagttgttc
     2461 ggcaccttga agccatagca gccgatgagg ttgtcccaga ccagccaccc gctgttgaac
     2521 cgccccatgc cccatgtatt gttcgagccg tccttgttcg gatcgttctt gctgaaggcg
     2581 acgagcatct tggcgatgct gtccgggttg gtcggttccg ccccgacatt ggtcgcccaa
     2641 tcggtgcggt agtacgggag gttgccggcg cgcagcgacg gattcggcac gccgaagatc
     2701 ttgccctgga acttcaggtt gtcccacatg taggagggga acgacgccag gttcttgaac
     2761 ggcttgatcg cgtcaccggt cacatacggc gtcagatcga ggaaggcgcc ctgcgccatt
     2821 gcctgatatt gctgcgtcgc gttctggccc ggattgatat agaagagatc cgggagatcg
     2881 ccacccgcga gataggctgc cgacttctcg ccgtagttcg gctgcggcgt catgtcgatt
     2941 tcccagctca cgccgagccg cttctccagc tcctgccagt actggttctg ccccttcggc
     3001 ggcggcggtg ggttgtagct gatcgtgaag gcgtgaacgg tgccgccgtt gccgggcgtc
     3061 ccgtcgtagc tcctggagac cgccggcgtc ttcagataga catccggcac cccttcgatt
     3121 cctgacggga cgacggtatc gctcgcgccg ggactcgccg cgggcgacgt ctccggtgtc
     3181 tgggcgaagg tgattcgcgg cccagcggca gcggcgaccg cgattccggc ggcaatcccg
     3241 gctcccttca cgatcgaacg gcgagacgtc gtgatggacg gcaacgtgat tgcgtcctgg
     3301 ttgtggatat ggctcttgtt ctgatcctgc gtcatcgagt gatcctccgg gcacacactc
     3361 accccggccc aggtcgttct gcaaccgggc gtcgtcgagt ggtccgacgt gtcgcgttat
     3421 gcgaatggaa acaggaaggc cagattctgc gtgagggcga cgcctccttt caccggttcg
     3481 cttcgacccg ggctctcagc ccttgatcga gcctgtcaac acacccttgg tgaaataccg
     3541 ctggaggaag ggatagacga tgaggatcgg cacggtcccg gcgacgacga tcgccatctt
     3601 gatcgtctgc gcaggcggcg gcgcctgtcc cgccttgaac tcgttggcgc tcgtcaggtt
     3661 cgaccccatc agcacgtact gccggagcac gacctggatc ggccacttcg tcgagtcgtt
     3721 caggtagaga atcgcgttga agaagttgtt ccagatcacc acgccgtaga agagggcgac
     3781 gaccgccagc accgctttcg agagcggcag cacgacctgc atgaagatct ggaagtcgtt
     3841 cgcgccatcg accttcgcgg cgtcgagcag ttcctgcggc aggttcatga agaagttgcg
     3901 gacgatgatg aggttccacg cgctgatcag cccgggaatg atgagggcgg agtaggaatt
     3961 gatcatcccg agctccttca cgaggaggta gctgggaatc atgcccgggc tgaggatcag
     4021 cgcaccgagg acgatgtaga gcacgacttt cgagccaagg accgaggtgc gcgacatccc
     4081 gtacgccatc gtcgtcgtga agagcatctg cgccgccgtg ccgacgatcg tcagcatcac
     4141 gctgatctgc agcgcgcggg tcacgacgcc accatcgaag atggcgcggt aggcatcgag
     4201 cgtcggatgc tcgggccaca gcacgagtcc cccccgctgc gcatcgaccg cagaggagaa
     4261 gctcatcgag acgacgtaga cgatcgggaa gagcatcgtg atcacgatca ccgcgagcac
     4321 gaggaatttt gcgatccgca tcgccggacc gggctcttcc atccagaccg gccgcgcgtc
     4381 gctgcgccgg ctcggccaga gcggcttctt gccgacaacg gttactgcct gtgtaaactc
     4441 cgcgtctggt tgtgtaacgg ccatcagagc aatccctcct caccgaagcg cttggcgagc
     4501 cagttcgccc cgagaatcag caacgcggcg accagactgc gcgtgagccc cacggctgct
     4561 gcgaaactcc actggccgga ctggaatccg agtgtgtaga cgagcgtgtc gatgacttcc
     4621 gcagtatggg cgccgtacgc ctggcgctgc aggaagtact gctcgaagcc ggtcgagaac
     4681 atcgatccca tgcggagaat caggagcagg atcacgacgc cacggatgcc cggcaacgtc
     4741 acgtcgagca ggcggcgcct gccgttggcg ccgtccatcg cggcggcttc gtagagctgg
     4801 atatcgatct tggtgagggc ggcgaggaag atgatcgttc cccagccgac ttccttccac
     4861 atcgtctcga ggatcattac cggcttgaac cagctcgggt ccgacatgat gttgaaggtc
     4921 tgcatcccgt gatcgctcaa cgcctggttg atcacacccg cgccgccgaa gacctgcgtc
     4981 cacagggcga cgacgatgac ccaggaaagg aaatatggga gatagacgat gctctggatc
     5041 gtccgcttga ttcgctcgct gatcagt
//
LOCUS       DASTMD010000247         3624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2613292, whole genome shotgun sequence.
ACCESSION   DASTMD010000247 DASTMD010000000
VERSION     DASTMD010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2613292"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            460..2835
                     /locus_tag="VFQ54_03715"
     CDS             460..2835
                     /locus_tag="VFQ54_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU0164125.1"
                     /translation="MDAASTTSGEAGNPLPRMQSQFVAHLDDGSLTVMPAPMIGRQRE
                     IQAIRTLLENPDIRLVVLTGPGGVGKTRIAITIAESEEPRQVAVVSLSSIGTPTLVLP
                     AIATALGIQGTTDAELRESVFHHLEGRDLLLVLDNFEHVIDAGPVLTELLTFAHNLTV
                     LVTSRVVLNLYGEYDFPVPPMLAPEFGDEGTIDPETVLAADAVQLFIDRAVAVLPAFD
                     VPREQIRDLLHIVRQLEGLPLAIELAASQIVTFTLPELIARLDPRLPFLERDRARRRV
                     LPDHSRTMRSAIAWSYDLLTPEEQALFCRLSLVIGSWTIDDAIAVAELDDDVAAVDLV
                     SSLVDKSLVRRVDLKGDTAHFDMLQTLREFGVEKLRENHEYDVAVDHYATYMLEFAAR
                     ASAHLTGATQMLWLDRLSNLHPDLRFVSAWFQGQDAPERALEMATAMWRFGYMRGHIS
                     ESVNNLLAALDRAPGRTALRAEALNAAGLLVNMQGDLARSGALHREARAIAEEIGYHK
                     AIGTAQLGLAELEVANRRFDEAQRLVLAAEELLTGSEDKRAVAVAKTNLGNLIWSMGS
                     LEQAEEIHQEAEVLYAAIGDERGVAWSATNVGRIAAERQEYVRAAASLQRAMTFYDKL
                     DDRSGIAETLEAFADIAHGTDDDTRAAIFLGAADRLRTAINHPVPGIDLERYRKLVAA
                     MRAHSERSYARGWARGKTLPLVKAIEMAMEYVVQLPDDGASAPAARSLLTTRELEVLR
                     LLRTGKSDREIGIELAIGVRTVQSHVANILAKLGVSSRTAAISRAIQDRLL"
     gene            3162..>3624
                     /locus_tag="VFQ54_03720"
     CDS             3162..>3624
                     /locus_tag="VFQ54_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164126.1"
                     /translation="MRDSDSHSRGTSSRSQPQEAPVRSPFKVRDLNSPILTEVWLVSA
                     VVAILGIRLYLQLTGYPQVGGSTLHIAHMLWGGLGMVIAFGILLIFASDVWKPAAAVI
                     GGAGFGAFIDELGKFITKDNDYFYRPAVALIYAVLVILFLISRTIDKLDRVT"
BASE COUNT          647 a         1176 c         1145 g          656 t
ORIGIN      
        1 agcttgcgcc gcgacagcag tgacgaccgg gtctcatgcg ttcgcatttc gatgtccgac
       61 atcctgagtt cccacttcgg ccgactccgg ccggtgcgcc catgaggtcc cgtgccgcca
      121 atcgacaggg atcctggtga ataaccggat cgcaatacac gtacccgcaa ggcacgtttt
      181 gccgatcaaa accagaatag accacacttt ggtagcaaat atggtcgatg tattggattc
      241 tgggcggtct ttgtatgcat ctatatgtga ttgatgagtt acatacgatg agacccgaat
      301 ggatcaagat gtacgtttgc cggataattt cgtactatca tcacggggac attcccaacg
      361 tacgtggcag gacgcatgaa caccgggagt tgaggcctgc gtgatcgatg agtcgatttc
      421 caccaccacg ctaccgatcg aagggcagat cgaagacgta tggacgccgc atcgacgacc
      481 tccggggagg caggcaaccc acttccgcgc atgcaatccc agttcgtcgc gcatctcgat
      541 gacgggagcc tgaccgtcat gcccgcaccg atgatcggtc gccagcgtga aatccaggcg
      601 atccggacat tgctcgagaa tcccgacatc cggctcgtcg tactcaccgg gccgggcggt
      661 gtcggcaaga cgcggatcgc gatcaccatc gccgagtccg aggagccgcg gcaggtcgcc
      721 gtcgtcagcc tctcctcgat cgggacgccg acgctcgtgt tgccggccat cgcgacggcg
      781 ctcggaatcc agggaacgac ggatgccgag ctccgcgaga gtgtcttcca tcatctcgag
      841 ggacgagacc ttctcctcgt gctcgacaac ttcgagcacg tcatcgatgc cggacccgtc
      901 ctgaccgaac ttctcacctt cgcccataac ctgaccgtgc tcgtcacgag tcgtgtcgtg
      961 ctcaatctct acggcgagta cgacttcccg gtgcccccga tgctggcgcc ggagttcggt
     1021 gacgagggga cgatcgatcc ggagaccgtt cttgcggcgg acgcggtgca gctcttcatc
     1081 gatcgcgccg tcgccgtgct gccggcattc gatgtgccga gggaacagat tcgtgacctc
     1141 ctccacatcg tgcggcaact ggagggcctg ccgctggcga tcgagctcgc ggcatcgcag
     1201 atcgtgacgt tcacgcttcc cgagttgatc gcccgcctcg atccacggct tcccttcctc
     1261 gaacgtgacc gcgcccgccg ccgcgttctt cccgatcatt cacgaacgat gcggagcgcg
     1321 atcgcctgga gctacgatct cctcactcct gaggagcagg cgctcttctg ccgtctctcg
     1381 ctcgtgatcg gcagctggac gatcgacgat gcgatcgccg tcgccgagct cgacgatgac
     1441 gtcgctgcgg tcgacctggt ctcttccctg gtcgacaaga gcctcgtgcg tcgcgtcgat
     1501 ctgaaaggtg ataccgctca cttcgacatg ctgcagacgc ttcgtgaatt cggtgtcgag
     1561 aagctccgcg agaatcacga atacgacgtc gcggtcgatc actacgccac ctatatgctc
     1621 gagttcgcgg cgcgtgcttc cgctcacctg accggcgcca cccagatgct ctggctcgat
     1681 cgtctgagca acctccatcc cgatctccgc tttgtctccg cctggttcca gggacaggac
     1741 gccccggaac gggcgctcga gatggcgacg gcgatgtggc gattcggcta catgcgcggg
     1801 cacatcagcg agagcgtgaa caatcttctc gcggcgctcg accgggcgcc ggggcgaacg
     1861 gcgctccggg ccgaggcgct caacgcggcc ggattgctcg tcaacatgca gggggacctc
     1921 gcccgctccg gtgcgctcca tcgggaagcg cgggcgatcg ccgaggagat cggctatcac
     1981 aaggcaatcg gcacggcgca gctcgggctt gcggagctgg aggtcgcgaa tcgccgcttc
     2041 gacgaggcgc agcggctggt cctggcggcg gaggaattgc tgaccggcag tgaggacaag
     2101 cgggcggtgg ccgtcgccaa gaccaatctc gggaacctga tctggtcgat gggatcgttg
     2161 gaacaggcgg aagagatcca tcaggaggcg gaggtgctct acgccgcgat cggcgacgag
     2221 cgcggcgtcg cctggtccgc gaccaatgtc gggcggatcg ccgccgagcg ccaggaatac
     2281 gtccgggcgg cggcgagcct gcagcgggcg atgaccttct acgacaagct cgacgaccgc
     2341 tctggaatcg ccgaaacgct cgaggcgttc gccgatatcg cgcacggcac ggacgatgat
     2401 acgcgggcgg cgatcttcct cggcgccgcc gatcgcctgc gaacggcgat caatcatccg
     2461 gtccccggaa tcgatctcga gcgatatcgg aagctcgtgg cagcgatgcg tgcgcacagc
     2521 gagcgctcct acgcgcgggg ctgggcccgg gggaagacgc tgccgctcgt gaaggcgatc
     2581 gagatggcga tggagtacgt tgtccagcta ccggatgacg gcgccagcgc gccggcggcg
     2641 cgatcgctcc tcaccacgcg cgaactcgag gtcctgcggt tgctgcgaac gggcaagagc
     2701 gatcgggaga tcggtatcga gttggcgatc ggtgtgcgca cggtgcagag ccacgtcgcg
     2761 aacatcctgg cgaagctggg cgtctcgtcg cggacggcgg caatctcgcg cgcgatccag
     2821 gacaggctcc tgtaggtctt cccaccgtcc gtcatccctg accgcaggga tcgcccgtac
     2881 gagatcgctc accaaaggtg atacatcggc gcgacggggc aaggaagcgt gcctgcccca
     2941 gtgcttcgag gcgggacata gaagtcccgc cgtactcaca accgcttcac gacgtatggc
     3001 cgcctcgctc cgcacgccgt tggaaccgcc agcactgcgt agcacgacct ggctcgaggg
     3061 cagccgcgaa ggtcgccggg acgatatgct atctccgcct gggtgaccgc gtgcgtcgcc
     3121 gcaacagggc gatccgttga gtcatcagga cgggaccgac catgcgtgac agcgacagcc
     3181 atagccgcgg cacatcctcg cgatcgcagc cccaggaggc gcccgttcgc tctccattca
     3241 aggtccggga cctcaacagt cccatcctga cggaggtctg gctcgtctcc gccgtggtcg
     3301 cgatcctcgg gatccgcctc tacctgcaac tgaccggtta tccgcaggtc ggtggttcga
     3361 cgctgcacat cgcccatatg ctctggggtg gcctggggat ggtgatcgcc ttcggcatcc
     3421 tgctcatctt cgcgagcgat gtctggaagc cggccgcggc cgtgatcggc ggcgccggtt
     3481 ttggcgcctt catcgacgag ctcgggaaat tcatcaccaa ggacaatgac tacttctatc
     3541 gtccggcggt cgcgctgatc tacgccgtcc tcgtcatcct cttcctgatt tcccggacga
     3601 tcgacaagct ggaccgggtg acac
//
LOCUS       DASTMD010000248         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1631297, whole genome shotgun sequence.
ACCESSION   DASTMD010000248 DASTMD010000000
VERSION     DASTMD010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631297"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1522
                     /locus_tag="VFQ54_03725"
     CDS             185..1522
                     /locus_tag="VFQ54_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0164127.1"
                     /translation="MKNTRISRRTFNGALTASIAALGAGVLSTETLHGVAAAAAQNGE
                     VRIEYWHRSGGDAATKWEDLAKQFNEQFAGKVQVTVVAQAGIQELNQKVRAAAAGGGM
                     PGALMADDPDVVQYLANDLIVPFDDYIADKTNGLTADQIAGFLPNQYNRQKLAIYGNK
                     RMAFPQANSAAAFFWNVDALKAAGLDAPVKTWQEFPDHARKLADANPGVIPYVFLDAG
                     AKFITTLKTYGIEWLKDDQKTANFDTPEALEVMTMWKQLSDDGLMQIVSDSATTVFAS
                     GKYVYLLDSSASSRGIATAKPSFTWDGGLLPQGAKDGKLRTETYGPINTLPKTTTEEQ
                     LAGWLWIKWLSTPDPLAQWVATTSYFPSEPAVADSDALKQFYTDYPVARTLIDSVAPY
                     ATIPAPHAALNEVRSTITADNVNEVLLGRLSPEDAVKKLQAEANDAISRANGN"
     gene            1648..2559
                     /locus_tag="VFQ54_03730"
     CDS             1648..2559
                     /locus_tag="VFQ54_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0164128.1"
                     /translation="MVRFGMSTGIGAGQPLAALRQSLERVAGVGFTHAEILSRNISAI
                     FNGQINTARLETLKEAIAGSPIAFTLHGSEITTSRGGNLMDISTSTERLIFEADLALA
                     RTIGASIVVYHSGTLRDIGNDTDAARAGMAAERETLRAMGDIAGESGITIAVENRVPI
                     GRTLLRHGYGISLERLADQINEIDHPQVGVCLDTGHAFLGAAYLGYDFLEQIRLIAPL
                     VSHIHFADNFGKVQLDERADPSEDVFAGLGDLHLLPGWGAIPFGELEQIAFPRRPIVN
                     LELRPQFVEHLQLASDTTRAFAERIGN"
     gene            2687..>3586
                     /locus_tag="VFQ54_03735"
     CDS             2687..>3586
                     /locus_tag="VFQ54_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0164129.1"
                     /translation="MVRFGISTGIGAGQPLSSLKNSLANLESVGFNHAEIGSRNVNVI
                     FNGQLNPVRLATLQEAMADSRLTFSVHGSEVSSSRGGNLMDISTPTERLIFEADLAFA
                     KAIGATVLVYHSGTLRDPGGDMDAVKAGMAAERDALRAMGDIAGESGITIAVENRDPA
                     GRYILRHSYGISLERLADQINEIDHPQVGVCLDFGHAYLGMAYLGQDFVEQCRQIAPL
                     VSHIHLTDNFGKVQLDEHADQSENLYAGLGDIHVLPGWGSIPFDEIAATISFPRQPIV
                     NLELRPQFFEHLQLASDVTRSFAA"
BASE COUNT          684 a         1254 c         1056 g          592 t
ORIGIN      
        1 aggactatcg ttatccattc ctgatttaat gactatcaac ttcgtttcac atagtgttca
       61 tatacaatta attcgtataa acccaaaccg atcctactat caacaaagcg ggcatattgc
      121 cacgctcgtt gaactgaatt cgccattggc tgatggcggg gaccgaaaca cggaaggaac
      181 cacgatgaag aacaccagga tttcacgtcg gacgttcaac ggcgcactga ccgcgagcat
      241 cgccgcactc ggtgccggcg tactgagcac cgagaccctt catggcgtcg ccgccgccgc
      301 ggcgcagaat ggtgaagtca ggatcgaata ctggcaccgc tccggtggcg atgccgcgac
      361 gaagtgggaa gacctggcga agcagttcaa cgagcagttc gcaggcaagg tccaggtgac
      421 ggtcgtcgcg caggccggaa tccaggagct caatcagaag gttcgcgcgg ctgccgctgg
      481 cggtggcatg ccgggcgccc tgatggccga cgatcctgat gtcgtccagt atctggcgaa
      541 cgacctgatc gttcccttcg acgactacat tgcggacaag acgaacggcc tcaccgccga
      601 tcagatcgcc ggtttccttc cgaaccagta caaccgccag aagctcgcga tctacggcaa
      661 caagcggatg gccttcccgc aggcgaacag cgccgcggcg ttcttctgga acgtggatgc
      721 gctgaaggcc gccggtctcg atgccccggt caagacctgg caggagttcc cggatcacgc
      781 ccgcaagctc gccgatgcca acccgggcgt catcccctat gtcttcctcg atgcgggcgc
      841 gaagttcatc acgacgctga agacctacgg gatcgagtgg ctgaaggacg accagaagac
      901 cgccaacttc gatacgccgg aggcgctcga ggtcatgacg atgtggaagc agctctcgga
      961 cgacggtctc atgcagatcg tgtccgacag cgccaccacc gtcttcgcct cgggcaagta
     1021 tgtctacctg ctggacagct ccgcctcgtc gcgcggcatc gcgacggcca agccctcgtt
     1081 cacctgggac ggcggtctcc tgccgcaggg cgccaaggat ggcaagctcc gcacggagac
     1141 gtacggcccg atcaacaccc tgccgaagac gaccaccgaa gagcagctcg ccggctggct
     1201 ctggatcaag tggctctcca ccccggatcc gctggcgcag tgggtcgcga cgaccagcta
     1261 cttcccgtcg gagccggcgg tcgcggacag cgatgccctg aagcagttct acaccgacta
     1321 tccggtcgcg cggacgctga tcgactccgt ggcgccgtac gccacgattc ccgcgccgca
     1381 cgccgccctc aacgaggtac gcagcacgat caccgcggac aacgtcaacg aggtcctgct
     1441 cggccgcctc tccccggagg acgcggtcaa gaagctccag gcggaagcga acgacgcgat
     1501 ctcccgcgcc aacggcaatt aacacggaga ccacacccct tgccctcgct cccgcgatga
     1561 tcgtgggcgg cgggaaccgg gttccccttt ctttctcgcg cccgtcgccg atctggcgac
     1621 gggcgcatcg ttttcaggag ttccatcatg gtccggtttg gaatgagcac cggcatcggc
     1681 gccgggcagc cgctcgcggc gctcaggcaa tcgctcgaac gagtggccgg ggtcggattc
     1741 acgcatgccg agattctcag ccgcaacatc agcgccatct tcaacggcca gatcaacacg
     1801 gcccgcctcg aaacactgaa ggaagcgatc gccggctccc ccatcgcctt cacgctgcac
     1861 gggagcgaga tcaccacctc ccgcggcggc aatctgatgg acatctcgac gtcaaccgag
     1921 cgtctgatct tcgaggccga tcttgccctc gcgaggacga tcggggcgtc gatcgtcgtc
     1981 taccacagcg gcacgctccg cgacatcggc aacgatacgg atgccgccag agcgggcatg
     2041 gccgccgagc gcgagacgct acgcgcgatg ggcgacatcg ccggcgagtc cgggatcacg
     2101 atcgcggtcg agaaccgcgt gccgatcggg cgcaccctgc tgcgccacgg atacggcatc
     2161 agcctcgagc gcctcgccga ccagatcaac gagatcgatc acccccaggt cggcgtatgc
     2221 ctcgataccg gtcacgcctt cctcggcgcg gcgtacctcg ggtacgactt tctcgagcag
     2281 attcgcctga tcgcgccgct cgtctcccac attcacttcg ccgacaactt cggcaaggtc
     2341 cagctcgacg agcgcgccga tccgagcgag gacgttttcg ccgggctcgg cgatctgcat
     2401 ctcctcccgg ggtggggcgc gatcccgttc ggcgagctcg agcagatcgc attcccgcgg
     2461 cggccgatcg tcaacctcga gctgcgcccc cagttcgtcg agcacctcca gctcgcctcg
     2521 gatacgaccc gcgccttcgc cgagcgcatc ggtaactaat gccacgacaa catctcgccg
     2581 cgacaggagc gggaagatcg ttgacgacgg gagacgatct cccctttcgc gctcgcattc
     2641 gtcgccgggt ctggcgacag acgcactcgt atcaggagtc cttcacatgg tcagattcgg
     2701 aatcagcacc ggcatcggcg ccgggcagcc gctctcctcg ctcaagaact cgctcgccaa
     2761 tctcgagagc gtcggtttca accacgccga gatcggcagc cgcaacgtca acgtcatctt
     2821 caatggccag ctgaatccgg tccgcctcgc gacgctgcag gaagcgatgg ccgattccag
     2881 actcaccttc agcgtccacg gcagcgaagt ctcgagctcc cgcggtggca acctgatgga
     2941 catcagcacc ccgaccgagc gcctgatctt cgaagccgat ctcgccttcg cgaaggcgat
     3001 cggggccacg gtgctcgtct accacagcgg caccctccgc gatcccggcg gcgacatgga
     3061 cgccgtcaag gccggcatgg cggccgagcg cgacgcgctg cgggcaatgg gcgacatcgc
     3121 cggcgagtcc gggatcacga tcgcggtcga gaatcgcgac ccggccggtc gctacatcct
     3181 ccgccactcg tacggcatca gcctcgagcg cctcgcggac cagatcaacg agatcgatca
     3241 cccccaggtt ggcgtctgcc tcgatttcgg ccacgcgtat ctcggcatgg cctacctcgg
     3301 acaggacttc gtggagcagt gccgccagat cgcgccgctc gtctcccaca tccacctcac
     3361 cgacaacttc gggaaggtcc agctcgacga gcatgccgac cagtcggaga acctctatgc
     3421 cggactcggc gacatccacg tccttccggg ctggggatcg atcccgttcg acgagatcgc
     3481 cgcgacgatc tcgttcccac gccagccgat cgtcaacctc gagctgcgcc cgcaattctt
     3541 cgagcatctc cagctcgcct ccgatgtgac ccgctcgttc gccgcg
//
LOCUS       DASTMD010000249         3854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2343912, whole genome shotgun sequence.
ACCESSION   DASTMD010000249 DASTMD010000000
VERSION     DASTMD010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2343912"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1075
                     /locus_tag="VFQ54_03740"
     CDS             197..1075
                     /locus_tag="VFQ54_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU0164130.1"
                     /translation="MVEDLVRTGGKRLRPLLLLLAAKAFTYERDRAVTAAAGVELLHT
                     ASLIHDDNIDRAALRRGQPTLNTELSSGAVILIGDFLFAQSAILAAATDDVRVVSIFA
                     NSLGAICDGQLRETLDAHRLNQDRAAYERRIYGKTASLFAGAAEMGAVVGGASDSEIR
                     ELRDFGGDVGMAFQIVDDILDLREGTDVLGKPSGNDIRQGVVTLPTMIFIENQDPEGS
                     VIRTLSDVIAGTETREEVIEDLISQIRRSGTLEEAEAIAADYSRRARARLSIVEDAEI
                     RGYLEEIVDLTLIRSR"
     gene            complement(1331..3049)
                     /locus_tag="VFQ54_03745"
     CDS             complement(1331..3049)
                     /locus_tag="VFQ54_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0164131.1"
                     /translation="MNGAQLLVRSLRASGVDHIFGLPGDTGMAFYDALYHEPAITHIM
                     TRDERSASFMADVYARVARRLGVCEGPSGGGATYIVPGVAEANGSAVPLLCMTSDTPV
                     GEDYRNVLTELDQVGLFRPVTKWSERVHRAASMPDAVARAIRLATSDRPGAVHLSLPA
                     DVLEDESEGQTRPDGASFGDTVPSSRPRADASEVERAAALIAQAERPVIVAGGGVMIS
                     GAWDALTAFADATNIPVATSINGKGAIAETHRAAIGIIGGNGARSYANAWIAESDLVI
                     FIGTRTESTTTSHWTLPKRPSPPATIHLDAASREISNTYPSSAFLLGDAKATLEDLLA
                     ALGDTRSLAARMAPRLDQLAIQRDQYWENVQAEIGSDSYPIKPQRLVAALRELLDDDT
                     IIVADPGTPTPYLGAQYELRRAGRTTVIPRAFGGLGYAIPGVVGAHYAAPGKRVVGVT
                     TDGSFGMSVGELETLSRLNLPIMLVHCSNGSFGWIKELQHLYHDQRYYSVDFSTVDYA
                     GIARGFGLKSWDVRDPADLTSTLQEALAYGGPTFVNVATLSQTVETPPVSGWEAAVAR
                     QAAQVS"
     gene            complement(3113..>3854)
                     /locus_tag="VFQ54_03750"
     CDS             complement(3113..>3854)
                     /locus_tag="VFQ54_03750"
                     /inference="COORDINATES: protein motif:HMM:NF017013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HEU0164132.1"
                     /translation="AACVSLILSDVLGNDPTVIASGPTVPRQPNPARALELLDRYRLR
                     ASVPAAVVELLEDSTTRGATAGASVRSDRDVWSIVGDNGLFLESLKTTAERHGLHAEI
                     IWRDREGEARDLAREFVELCAGMSEDVDCVLGGGEATVAVAGDGLGGRNTEFALAAAV
                     ELAERRLPWAIASLASDGDDGANEHAAGGVVDNMTIGRGIAAGLDARDALERNDSGAY
                     LRAVDAMFTGPPTGTNVNDAYIGIRLAK"
BASE COUNT          672 a         1309 c         1209 g          664 t
ORIGIN      
        1 ccacgaacaa gataatctcc atggagatgt gtcggcgcgc aaccgggaca cacacatggg
       61 aatcccactc gcgggatggg gagaatcagt gagagccaca acggcggacg ttttcgaggc
      121 aatgcgagag gacctggaac gggtcgagcg gcgcctcatg gaggcggccc gcatcgattt
      181 ccctattgtc tcttccatgg ttgaagatct ggtgagaaca ggcggcaaac ggctgcgacc
      241 gttgttgctg ttgctcgccg ccaaggcgtt cacctatgag cgcgatcgcg ccgtaacggc
      301 tgcggcaggc gtcgaactcc tgcacacggc ctcccttatt cacgacgaca atatcgaccg
      361 cgccgcgctt cgtcgcgggc agccgacact caacaccgaa ctgagctcgg gcgcagtcat
      421 cctgatcggc gactttctct ttgcccagag cgcaattctc gccgctgcca ctgacgacgt
      481 acgcgtcgtc tcgatcttcg cgaattcgct cggggcgatc tgcgacggcc agcttcggga
      541 aacgctcgat gcccaccgtc tcaaccagga tcgtgccgcc tatgagcgcc gcatttatgg
      601 aaagacggcg tccctctttg ccggtgccgc cgagatgggc gccgtcgtcg gcggagcgtc
      661 cgatagcgaa attcgtgagc ttcgcgactt cggcggcgat gtcggcatgg ccttccagat
      721 cgtggatgac atcctcgacc tgcgggaggg aaccgacgtc ctcggcaagc cgtcgggaaa
      781 cgacattcgc cagggcgtcg ttacactgcc gacgatgatc ttcatcgaga atcaggatcc
      841 tgaaggcagc gtcattcgca ccctctccga cgtgatcgcc ggcacggaga cgcgcgaaga
      901 agtcatcgag gatctgatct cgcagatccg ccgctccggg acgctcgagg aagccgaggc
      961 aattgccgcc gactactcac ggcgcgcccg cgcccggctt tcgattgtcg aggacgccga
     1021 gattcgcggg tacctcgagg agatcgtcga tctcacgctg atccgctccc gctgagcgac
     1081 gaagacgtcg atacatacga cgaacgaacc cccggtcgca gatgcgaccg gggttcgtgt
     1141 gccggctccc cgccggtgag ccccttccgc gtggtctacc aggcgtgctg ctggctcaga
     1201 tgcagagttc ctcatgcacc cggtcgttcg ggagtgtgtc gaaggggcgg gaccgccgcc
     1261 ggaccgtggt ccggacggcc tgtcccgcct catgcaccct cagggatcgc tccgcctgcg
     1321 tccaactccc ctacgagacc tgggcagcct gccgcgcgac agccgcttcc cagcccgaaa
     1381 ccggcggcgt ctcgaccgtc tggctcagcg tggcgacatt gacaaaggtc ggcccaccgt
     1441 aggcgagcgc ctcctgcagc gtcgatgtca gatcggccgg atcccgcacg tcccaggact
     1501 tcagcccgaa tccgcgtgcg atccccgcgt aatcgacggt cgagaagtcg accgagtagt
     1561 accgctgatc gtggtagaga tgctgcaatt ccttgatcca gccaaacgat ccgttactgc
     1621 aatggacgag catgatcggc aggttgaggc ggctgagcgt ctccagctcg ccgaccgaca
     1681 tcccgaagct gccatcggtg gtcacaccga cgacgcgctt gcccggcgcg gcgtagtgcg
     1741 cgccgacgac gcccggaatc gcgtaaccga gcccgccgaa cgcgcgcggg atcaccgtcg
     1801 tccggcctgc tcgccggagc tcgtactgag cgccgaggta gggcgtcggc gttcccgggt
     1861 cggccacgat gatcgtatcg tcgtcaagca gttcacggag ggcggcgaca agacgctgcg
     1921 gcttgatcgg atagctgtcg ctgccgattt ccgcctgcac gttctcccag tactggtcgc
     1981 gctggatcgc cagctggtcg agtcgtggcg ccatcctggc cgccagcgaa cgcgtgtcac
     2041 cgagtgcggc gagcagatcc tcgagggtcg ccttcgcgtc gccgagcaga aacgcgctcg
     2101 acggataggt gttgctgatt tcccggctcg cggcatcgag atggatcgtc gccggcgggc
     2161 tcggtcgctt tggcagcgtc cagtgcgacg tcgtcgtcga ctcggtgcgc gtgccgatga
     2221 agatcacgag atcggattcg gctatccagg cgttcgcata cgagcgcgcg ccgttcccgc
     2281 cgatgatgcc gattgccgcc cggtgggtct ccgcgatcgc ccccttgccg ttgatgctcg
     2341 ttgccaccgg gatattcgtg gcgtccgcga aggcggttag ggcatcccag gcgccggaga
     2401 tcatcacgcc gccgccggcc acgatcacgg ggcgctcggc ctgtgcaatc agtgccgcgg
     2461 cccgttcgac ctcggacgca tccgcgcgcg gtcgggaact tggcacggta tcgccgaacg
     2521 acgcgccgtc gggacgagtc tgaccctcgc tctcatcctc caggacatcg gcaggaagtg
     2581 aaagatggac cgcaccgggt cgatcgctgg tcgccaacct gatggcgcga gcgaccgcat
     2641 cgggcatgct cgccgcgcga tgcacgcgct ccgaccactt tgtgacgggg cggaagagcc
     2701 cgacctggtc gagctcggtg aggacgttcc gatagtcctc accgactggt gtatccgagg
     2761 tcatgcagag cagcggcacg gcagagccgt tcgcctccgc aacccccggc acgatgtagg
     2821 tcgcgccacc accgctcggc ccctcgcaga caccgagacg acgcgccacg cgcgcataga
     2881 cgtcggccat gaatgatgcc gatcgctcat cgcgggtcat gatgtgcgtg atcgccggtt
     2941 catgatagag ggcgtcgtag aacgccattc ccgtgtcacc cggcagaccg aagatgtgat
     3001 cgaccccgct cgcccgcagc gaacgcacca ggagctgcgc tccgttcatg gaatcttccc
     3061 tttcagatac gtcggtcatg cggatcggga gcaccgtcga tccccgcaca ggtcatttcg
     3121 ccaatcgaat tccgatgtac gcgtcgttga cattcgtccc cgtcggcgga ccggtgaaca
     3181 tcgcgtccac ggcccgaaga tacgcgccgc tgtcgttgcg ttcgagcgca tcacgggcat
     3241 ccagtcctgc ggcgatcccg cgaccaatcg tcatgttgtc gacgaccccg cccgcggcgt
     3301 gctcgttcgc accgtcgtca ccatcggagg caagactcgc gatcgcccag ggcagccttc
     3361 gctccgccag ctcgaccgcc gcggcaagtg cgaactccgt gttcctgccg ccgagaccat
     3421 cgccggccac tgcgacggtc gcttcgccac ccccgagtac acaatcgaca tcttcggaca
     3481 ttccggcaca aagctcaacg aactcgcgcg ccagatccct cgcctcgccc tcgcgatcgc
     3541 gccagatgat ctcggcatgg agaccatgtc gttccgccgt cgtcttcagc gattcgagga
     3601 agaggccgtt gtcaccgacg atcgaccaga catcccggtc tgaccggacc gaggcacccg
     3661 cggtcgcccc gcgagtcgtc gaatcctcga gcagttcaac cactgccgcc gggacggatg
     3721 cccgcagccg gtaccgatcc agcagttcca gcgcccgcgc tgggttcggc tgacgtggca
     3781 ccgtcggtcc gctcgcgatg acggtcggat cgttgccgag aacgtcggag agaatcaagg
     3841 agacgcaggc ggcc
//
LOCUS       DASTMD010000250         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_112112, whole genome shotgun sequence.
ACCESSION   DASTMD010000250 DASTMD010000000
VERSION     DASTMD010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112112"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VFQ54_03755"
     CDS             <1..1051
                     /locus_tag="VFQ54_03755"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU0164133.1"
                     /translation="SDTPRVQPEHRKEEILSFINSGLEDFSISRQALRGRGWGVPVPG
                     DPEQVMYVWVDALSNYITALDYATSGPLYRHYWTEGDERVHIIGKDILRFHAVYWPAL
                     LISAGEPIPTTVNVHEFLTSNGEKMSKSKGNVIDPLQLVADYGNDATRYWLLREMPRT
                     SDGDFTYERLVGRYNQDLANDLGNLVNRSVGMLQRYRGGIVPGVGARDGVLQQVATEI
                     PARIYDGLDRFDFRSAITAAWELVTAANRYIDDTKPWGIAKAAKEGDPDADARLDIVL
                     ADLIETIRLLAVYLAPFIPVGAAKIATQVGFVLDEAGDATSRDWTDALAGRQLPAAAP
                     IFPRIEVPVEEPVVG"
     gene            complement(1192..1809)
                     /locus_tag="VFQ54_03760"
     CDS             complement(1192..1809)
                     /locus_tag="VFQ54_03760"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEU0164134.1"
                     /translation="MFSSSTIFDFVDIFWVFVGALTGALVARRQQYDITGIFGIALAS
                     GLGGGLIRDTLLQDGPPLALVNHTYLPTVVVATVAGAFFGGRFDQLKRTILISDAVTL
                     GFFAVAGCLRTFDAGLDAWPAVLLGVTTAVGGGALRDVLMGETPMIFRRGELYALAAL
                     IACFAVVICRDLDLPRTVVVTVGIGVGIVIRLGSVKYHWYSWVPR"
BASE COUNT          390 a          662 c          646 g          335 t
ORIGIN      
        1 ctccgacacc ccccgcgtgc agccggagca tcggaaggag gagatcctct ccttcatcaa
       61 cagcggactc gaggacttca gcatctcccg gcaggcattg cgcggtcggg gctggggtgt
      121 tcccgtcccg ggcgatcccg agcaggtgat gtacgtctgg gtcgatgcgc tcagcaacta
      181 catcaccgcg ctcgactacg cgaccagcgg gccgctttat cgccactact ggacggaggg
      241 cgacgagcgt gtccacatca tcggcaagga catccttcgc ttccacgccg tctactggcc
      301 ggcgttgctg atctctgccg gcgagccgat cccgacgacg gtcaacgtcc acgagttcct
      361 cacctcgaac ggcgagaaga tgagcaagag caaggggaac gtgatcgatc ccctgcagct
      421 ggtcgcggac tatgggaacg acgccacccg ctactggttg ttgcgggaga tgccgcgcac
      481 gagcgatggt gacttcacct acgagcggct ggtcggtcgc tacaaccagg atctcgccaa
      541 cgatctcggc aacctcgtca atcgcagcgt gggaatgctc cagcgctacc gcggcggcat
      601 cgtgccgggg gtcggcgcgc gcgacggtgt gctgcaacag gtcgcgaccg aaatcccggc
      661 gcggatctac gacggtctcg atcgcttcga ttttcggagc gcgatcactg cggcgtggga
      721 actggtgacg gcggcgaatc gctacatcga cgacaccaag ccatggggaa tcgcgaaagc
      781 ggccaaggag ggagatcccg acgccgatgc ccggctcgac atcgtgctgg ccgatctgat
      841 cgagacgatc cggctcctcg cggtctatct cgcgccgttc attccggtcg gcgccgcgaa
      901 gatcgcgacg caggtcgggt ttgtgctgga cgaggccgga gacgcgacat cgcgcgattg
      961 gaccgatgcg ctggccggtc ggcagttgcc tgccgccgcg ccgatcttcc cgcgaatcga
     1021 ggttccggtc gaggaacccg tcgtcggctg atccaccggg aggcgacgaa cgatcgttcg
     1081 tcgcctcccg gcctggtcac gtcccgtgat ggatcgtgtc aacggccaat gccgtctccg
     1141 gcggatgccg tcacttcgcg tgattacccg gatcccgtaa acatcctgat gctagcgggg
     1201 aacccacgaa taccagtggt atttcaccga gccgagacgg atcacgatcc cgactccgat
     1261 cccgacggtg acaaccaccg tcctcggcaa atcgaggtcg cggcagatca cgacggcgaa
     1321 gcaggcgatc agcgcggcaa gcgcatagag ttcgccgcgc cggaagatca ttggtgtctc
     1381 gcccatcaac acgtctcgca atgcccctcc ccccaccgcc gtcgtcacgc ccaggaggac
     1441 cgctggccac gcatcgagtc cggcgtcgaa ggtccgcaga caccccgcca ccgcgaagaa
     1501 gccgagagtc acggcatcgg agatgaggat ggttcgcttc aactggtcga agcgaccgcc
     1561 gaagaaggcg ccggccacgg tcgcgacgac gaccgtcgga agataggtgt gattgacgag
     1621 cgccagcggc ggaccgtcct ggaggagcgt gtcgcggatc aggccgccgc ccagtccgct
     1681 cgccagggcg atgccgaaga ttccggttat gtcatattgc tggcgacgtg cgaccagggc
     1741 gccggtcagc gcaccgacga acacccagaa gatatcgaca aagtcgaaga tcgtgctcga
     1801 agaaaacatg cgcttcgggt tccctctggc gtggaatgct cgcgacgctc tcgcctggtc
     1861 tctgccccat atccgtcgtc ctgctgagtg taccgaatcg cgccgccagt tctgagccaa
     1921 ttgcgccctc acctgcttcc tgtgagaatc gtcctgttgg gaaaggcggg gaaatcagga
     1981 acggagcgat cgatggccgg cacgacgcga tgagaatcgg aaccacgacc acc
//
LOCUS       DASTMD010000251         1578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1184623, whole genome shotgun sequence.
ACCESSION   DASTMD010000251 DASTMD010000000
VERSION     DASTMD010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184623"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="VFQ54_03765"
     CDS             <1..1502
                     /locus_tag="VFQ54_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0164135.1"
                     /translation="DSGSGKSTFLRALNGLVPHFSGGTFGGVVRVGGLDTRRHPPREL
                     SRIAGFVFQDPEAQMVTDRVDDEIAFSLEQHGIDPLVMRKRVEEMLDVLGIVPLRHRS
                     PATLSGGERQRVAVASALALHPRLLLLDEPTSQLDPWGAEEVLTALRRLNEELGLTIV
                     MAEHRLERTLHHADLVLKFGAAPGVVRGTPRGMAVHLESASLPPVTRLGLAVGSDPVP
                     LTVKEGRGVAAAFSLSHTTYPGRMRSGPAGASALELEGITVELDRRDVLKDLSLTIGE
                     GEIVALMGRNGSGKSTLLRTMLGLEQPRKGRLRIGGVDRTGKDPAELRGLIGYVPQRP
                     DLLLYHELLGDELRHAMKLRGIDGPIEPWLARLGLGGMADRHPRDLSGGERERAAIAT
                     VLAAEPRILLLDEPTRGMDARHKVELMSLLAELAAEGKAIVIATHDVELVAWFASRVV
                     LLGDREIVADGPPRKVLVDSLTFTTQMNTLFGGEILTVEDVLARLPHPG"
BASE COUNT          248 a          496 c          562 g          272 t
ORIGIN      
        1 gagactccgg ttctggaaaa tcgaccttcc ttcgcgccct caacggcctg gttccccact
       61 tctcgggggg gacattcggt ggggtcgtgc gtgttggcgg cctggatacg cgacggcatc
      121 caccgcgcga gctgagccgg atcgccggct tcgtgtttca ggatcccgaa gcgcagatgg
      181 tgacggatcg cgtcgatgac gagattgcct tctcgctgga acagcacggg atcgatcccc
      241 tggtgatgcg gaagcgagtc gaggagatgc tcgatgtcct cgggatcgtg ccgctgcgac
      301 atcgatcgcc ggccacactc tccggcgggg agcgtcaacg cgtcgccgtc gcctccgcgt
      361 tggcgctcca cccgcgtctg ctgctgctcg acgagccgac gagtcagctc gacccgtggg
      421 gagcggaaga agtgctgacc gccctcaggc ggctgaacga ggaactcggc ctgacgatcg
      481 tgatggcgga gcaccggctg gaacggacgc tgcatcacgc cgacctcgtc ctgaagttcg
      541 gggcggctcc cggtgttgtg cgtggcacac cgcgcgggat ggcggtgcac ctggagtcgg
      601 cgagcttgcc gccggtgacg cgattggggc tggcggtcgg gagcgatccg gttccgttga
      661 cggtgaagga ggggaggggc gttgccgccg cgttctcgct ctcccacacg acatatcccg
      721 gcaggatgcg atctggtcct gccggtgcct cagcgctgga actcgagggg atcaccgtcg
      781 agctggatcg gcgggatgtg ctgaaggacc tctcgctcac gatcggcgag ggggagatcg
      841 tcgccctgat ggggcggaac ggcagcggca agtcgacgct gctccggacg atgctcgggc
      901 tggaacaacc gcgcaagggg cgtctccgga tcggcggcgt cgatcgcacg ggaaaggatc
      961 cggcggaact tcgcggtctg atcgggtatg tcccgcaacg cccggacctg ctgctctatc
     1021 acgaactgct cggcgacgag cttcggcacg cgatgaaact gcgcgggatc gatggcccga
     1081 tcgaaccgtg gctcgcgcga ttgggactgg gcggcatggc cgatcggcac ccgcgcgatc
     1141 tcagcggtgg ggagcgcgag cgggcggcga tcgccaccgt tctcgccgct gaaccgcgga
     1201 tccttctcct cgacgaaccg acacgtggga tggacgctcg tcataaggtc gaactgatgt
     1261 ccctgctggc ggaactggcg gcggaaggga aggcgatcgt gatcgcaacg cacgatgtcg
     1321 agctcgtcgc ctggtttgcc tcgcgcgtgg tcctgctggg cgatcgcgag atcgtcgccg
     1381 acggcccacc ccgcaaggtc ctcgttgact cgctgacgtt caccacgcag atgaatacgc
     1441 tcttcggcgg cgagatcctg accgtggaag acgtgctcgc ccgactcccg catcccggat
     1501 gaatcgcccg ggcgcgatca ctgcgggtcg agcgccgcct tcgtgtacgt gttgtcgggg
     1561 tcccagagaa gccagccc
//
LOCUS       DASTMD010000252         3380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_915833, whole genome shotgun sequence.
ACCESSION   DASTMD010000252 DASTMD010000000
VERSION     DASTMD010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915833"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFQ54_03770"
     CDS             complement(<1..183)
                     /locus_tag="VFQ54_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164136.1"
                     /translation="MRSMRQLLPVIRLPFLLRPLAALALAFLLVGPGLTSAPPAAAQP
                     HKLKVVASFSILGDWIQ"
     gene            403..1524
                     /locus_tag="VFQ54_03775"
     CDS             403..1524
                     /locus_tag="VFQ54_03775"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEU0164137.1"
                     /translation="MDAAISRPSLTRAIIALLLVTMTALTPFARSTKVFAATQSDDEA
                     AVRVAVSDLNRFEQDGNPEALYDRMAPDARNVMPRSVWVAWYGLEPRLVPTATPAVSS
                     IDFGSWTWDVTGDDYDDVATVAVTQTGTLNGKSTSQNQTLHFLSVDGKWKWFPLDAPS
                     DIEDAVDAADAIPAYKTNFETSNYAAIDTFWAQNFSAVGLDYKRPAHITSVTRNLQVA
                     PGCGTAKDVADAGIYYCTISNSIYYSPDMKQSVLDYVGDWAWTEILAHEWGHYIQDLV
                     NIDQTSQPELDGGYYSIELELQADCLSGMFMQNEMALVKLDDGALDEVSSIISVSGDA
                     TDTPWDERDAHGTNEQRQASFDNGFENGFTGCRIDLTSD"
     gene            1898..3208
                     /locus_tag="VFQ54_03780"
     CDS             1898..3208
                     /locus_tag="VFQ54_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164138.1"
                     /translation="MSIAAPQTSPLDVFSHGVLPGWARLRQTIDGEDVGSIDDAIARE
                     FQRQEVRDAIGQGTRVALTAGSRGVDRIAEVLRAAVAQVKLLGGEPFVIPAMGSHGGA
                     TAEGQRALIAHYGVVEEFIGCEIRASMETVELGVLEGGVPVWFDKTAYEQADVVIPVG
                     RVKPHTDFRGPTESGLMKMIAIGLGKQHGASWFHSQGIGTFGDLIPRVGKFTLSKVNI
                     PFGIALVENGYSKLAVIEVVPAKGMYEREIELLKIAFAKLAQLPAIDGADVLIVDKIG
                     KDISGDGADPNVIHRDVAGVIDFTNAKPRIQRYIVRDITHDTEGNATGIGMFDFALRR
                     VVDKMDQIPTAMNMITAKSPQGARIPITVDTDRQALQLAVASAIKVVEGEAKILRIAS
                     TKQMDTFYASETLIPDLLATGQVELETQVAPIAFDEDGMFVDGF"
BASE COUNT          646 a         1048 c         1113 g          573 t
ORIGIN      
        1 ctggatccag tcgccgagga tgctgaagct ggcgacgacc ttcagcttgt gtggctgggc
       61 ggcggcgggc ggtgccgacg tcagaccggg accgacgagc aggaaggcga gggcgagcgc
      121 cgcaagcggg cggagcagga atggaaggcg gatcacagga agaagctgcc gcattgaacg
      181 catggtcggg ttctttcttc tgcaggactc gagaagctcg atccgcgcct gacgttcgag
      241 gcgtgaatcg gaccaccttt actattgata cagtgtgtca tcataggttt ggcctggacg
      301 gcatgtcaac cgcgatccgc tcgatcggaa ccggggtcgc gttcttcgcg tcctatcact
      361 agcggatacc tgattcgtgg cgcacttgga gacaggactg acgtggacgc tgccatatct
      421 cgaccctcgc tgacccgcgc aatcatcgcg ctgctgctcg tgacgatgac ggcgctgacc
      481 ccattcgcca gatcgacgaa ggtcttcgcc gccacccaga gcgatgacga ggccgccgtt
      541 cgcgtcgcgg tttccgatct caaccggttc gaacaggacg gcaatccgga ggcgctctac
      601 gaccgcatgg cgccagatgc ccgcaacgtc atgccgcgca gcgtctgggt tgcctggtac
      661 gggctcgagc cgcgtctggt tccgaccgcg acgccggcag tctcctccat cgacttcggg
      721 agctggacct gggacgtcac cggcgacgac tacgatgacg tcgcgacggt cgcggtgacc
      781 cagacgggca ccctgaacgg caagagcacg agtcagaacc agacgctgca cttcctcagc
      841 gtcgatggca agtggaaatg gttcccgctc gatgcgccgt cggatatcga ggatgccgtt
      901 gacgcggcgg atgcgatccc ggcctacaag acgaatttcg agaccagcaa ctacgccgcg
      961 atcgacacgt tctgggcgca gaacttcagc gcggtggggc tcgactacaa gcggcccgcc
     1021 cacatcacgt ccgtcacgcg caacctgcag gtggcgccgg gctgtggcac ggcgaaggat
     1081 gtcgccgatg ccggcatcta ctactgcacg atcagcaact cgatctacta ctcgcccgac
     1141 atgaaacaga gcgtgctcga ctacgtcggt gactgggcgt ggacggagat cctcgcccac
     1201 gaatggggtc actacatcca ggacctcgtc aacatcgacc agacatcgca accggaactc
     1261 gacggcggct actacagcat cgaactcgag cttcaggccg attgcctctc ggggatgttc
     1321 atgcagaacg agatggcgct cgtgaagctc gacgatggcg cgctcgatga ggtgagttcg
     1381 atcatctcgg tctcgggtga tgcgaccgac accccgtggg acgagcggga cgcccatggg
     1441 acgaacgagc agcgacaggc gtcgttcgac aacggcttcg agaacggctt caccggctgc
     1501 cggatcgatc tcacctcgga ctagcgaggc gcggcgcggg cgaggcggcg caaaccctcc
     1561 ggcgcgacga ggtcaggcta cgcctgacgg agggacatgg cgaaggtctg cccggatcct
     1621 ggaacgtcgt acccgcgaag atcaggcggg acattcctcc gtccaacgac cggaggtctc
     1681 ccaggcaaga aacgccgatc gccggtcatt ggactccgta gtcccgcccc acgtacgacc
     1741 gccgatcgcg acgtccccta ccggatcgat cgtccatgcg gcgcgcgacc tgttcgacga
     1801 gcaccataaa cgcggcgtcg gctctacaat ggcgccatcg tttgccatcg ccacccgtga
     1861 cgagcctcaa cgccgaacgc agcaaggagt tttctcgatg agcatcgccg ctccccagac
     1921 cagcccgctc gatgtctttt cgcatggtgt cctgccggga tgggcccgcc ttcgccagac
     1981 gatcgacggc gaggatgtcg gttcgatcga cgatgcgatc gcccgtgaat tccagcggca
     2041 ggaagtccgt gatgcgatcg gacagggcac gcgggttgcg ctgaccgccg gcagtcgtgg
     2101 tgtcgaccgc atcgccgagg tgctgcgagc ggcggtcgcc caggtgaagc tgctgggcgg
     2161 agaacccttc gtgatcccgg caatgggcag tcatggtggc gcaaccgccg agggccagcg
     2221 ggcgttgatc gcccactatg gcgtcgtcga ggaattcatc ggctgcgaga ttcgcgcgag
     2281 catggagacg gtcgagcttg gcgtgctcga agggggagtc ccggtctggt tcgacaagac
     2341 cgcctacgag caggccgatg tcgtgatccc ggtcggtcgc gtcaagccac acaccgactt
     2401 ccgggggccg acggaatccg ggctcatgaa gatgatcgcg atcggtctcg gcaagcagca
     2461 cggcgcgagc tggttccaca gccaggggat cgggacgttc ggcgatctca tcccgcgcgt
     2521 cggcaagttc acgctgagca aggtcaacat ccccttcggc atcgcgttgg tggagaacgg
     2581 ctacagcaag ctcgcggtga tcgaggtcgt ccccgcgaag gggatgtacg agcgtgagat
     2641 cgagttgttg aagatcgcct tcgcgaagct cgcgcagttg ccggcgatcg atggggcgga
     2701 tgtcctgatc gtcgacaaga tcggcaagga catcagcggc gacggcgccg atccgaatgt
     2761 catccatcgg gatgtcgccg gggtgatcga cttcacgaac gcgaagccgc gcatccagcg
     2821 ctacatcgta cgcgacatca cccacgacac ggaggggaac gcgaccggga tcggcatgtt
     2881 cgatttcgcg cttcgccgtg tggtcgacaa gatggaccag atcccgaccg caatgaacat
     2941 gatcacggcg aagtctcccc agggtgcacg gatcccgatc accgttgata ccgatcgcca
     3001 ggcattgcag ctggcggtgg cgtcggcgat caaggtcgtc gaaggggagg cgaagattct
     3061 tcgcatcgcc agcacgaagc agatggatac gttctatgcc tccgagaccc tgatcccgga
     3121 tctcctcgcg acgggtcagg tcgagctgga gacgcaggtt gcgccgatcg cgttcgacga
     3181 ggacgggatg ttcgtcgacg gcttctagcc ccttcattcg tcctctcgag cttgtcgaga
     3241 agttcctgcg ttcgtagggg cgagcctggt gctcgccctc cgccggaggc ggactccaac
     3301 ggaaacgtcc acctcaaacg gcgttcgggg aggcgggtcg gctgcgccgc attgacccgg
     3361 aagggccgtg gggacgtgaa
//
LOCUS       DASTMD010000253         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1987630, whole genome shotgun sequence.
ACCESSION   DASTMD010000253 DASTMD010000000
VERSION     DASTMD010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1987630"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1065)
                     /locus_tag="VFQ54_03785"
     CDS             complement(64..1065)
                     /locus_tag="VFQ54_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0164139.1"
                     /translation="MTDLTPIRWGILGTGRIARKFTHDVQAAAPGAVVTAVGSRMQET
                     ADAFGEEFGIASRHGSYEALAADPEVDVVYVATPHPWHFEASMVCLNAGKAVLCEKAF
                     TMNARQAKEMVALARSKNVFLMEAMWTRFRPAMVKVRDLIAEDAIGEVQYLSAAVGWK
                     SPFETTSRVFRKDLGGGILLDGTIYPISFAYNILGTPSETLTVGVLGEAETDEQETIV
                     FSYPSGAQATIFGSLRAGLNGPAIIAGSKGKIEITHQWWEPWQVVLYADGQEPQTFDF
                     REDGTLGYQYEAIAVGEALRAGKTETAIMPLDETVEIMEMMDSLRAKWGLTYPADAE"
     gene            complement(1272..2273)
                     /locus_tag="VFQ54_03790"
     CDS             complement(1272..2273)
                     /locus_tag="VFQ54_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0164140.1"
                     /translation="MSDLSTIRWGILGTGGIAHKFTHDVQAAVPGAVVTAVGSRSQET
                     ADAFGDEFGIPNRHPSYELLAADPEVDAIYVATPHPWHHEATILSLNGGKAVLCEKGF
                     AMNARQAREMIDTARAKNLFLMEAMWTRFRPAMVKVRELIADGAIGTPQIVSASIGWL
                     GGNNPASRLYNKDLGGGLLLDGCIYPISFAFMVLGTPNQISSSVVIGETGVDEQESIS
                     FGYPSGAVASLTGSFRATPNNIAVVAGEKGRIEVSRRFWEPKQITLFPLNGEPEVFDF
                     PEENTTGYQYEAIAVGEAIRAGKTESPIMPLDETLTIMQTMDTLRAQWGLTYDADLA"
BASE COUNT          434 a          833 c          845 g          468 t
ORIGIN      
        1 tctatgtccc gcctcagact ggtactcgag cagatgcgca ttgccgacag tgtcgccgtc
       61 gacctattct gcgtcggcag gatacgtcaa accccatttc gcgcgcaggg aatccatcat
      121 ttccatgatc tcgaccgtct cgtcgagcgg catgatcgcg gtctcggtct tgccggcgcg
      181 cagcgcctcg ccaacggcga tcgcctcgta ctggtacccc agcgtgccgt cctccctgaa
      241 gtcgaaggtc tgcggctcct gcccgtcggc gtagagcacg acctgccacg gctcccacca
      301 ctgatgggtg atctcgatct tgcccttcga tccggcgatg atcgccgggc cgttgaggcc
      361 cgcgcggagt gacccgaaga tcgtcgcctg ggcgccggac ggataggaga agacgatcgt
      421 ctcctgctcg tcggtctcgg cctcaccgag cacaccgacg gtcagggtct cactcggcgt
      481 cccgaggatg ttgtaggcga aggagatcgg gtagatcgtg ccgtcgagga ggattccacc
      541 gccgagatcc ttgcggaaga cgcgactcgt cgtctcgaac gggctcttcc agccgactgc
      601 ggcggacagg tactggacct cgccgatcgc gtcctcggcg atcaagtcac gcaccttcac
      661 catcgccggg cggaagcgcg tccacatcgc ctccatcagg aagacattct tcgatcgcgc
      721 gagcgcgacc atctccttcg cctggcgggc gttcatcgtg aacgccttct cgcagagcac
      781 ggccttgccg gcgttgaggc agaccatcga cgcctcgaag tgccacggat gaggtgtcgc
      841 aacgtagacg acgtcgacct cgggatcggc ggcgagcgcc tcatagctgc catgacgact
      901 ggcgatcccg aactcctcac cgaaggcgtc ggcggtctcc tgcatgcggg atccgacggc
      961 ggtcacgacc gctcccggcg cggccgcctg cacgtcgtgc gtgaacttgc gggcgatcct
     1021 gccggtgccg aggattcccc agcgaatggg tgtgaggtct gtcacgggtt atgtcgtcct
     1081 ttcctaactg agttacgaag gcgggacacg gaggtaccgc cgtacgaacg ctcgcgcggg
     1141 ccgtggcccg cgccaatggg atcacgtatg cgcgtgcacc gagcatccaa tcagcgttcg
     1201 tgagggcggg acttctatgt cccgcctcat gctccgaaca aactgcttcg catatcctgc
     1261 cgcctcgaac cctacgccag atctgcgtcg taggtgagcc cccactgcgc gcggagcgta
     1321 tccatcgtct gcatgatcgt cagcgtctcg tcgagcggca tgatcgggga ttccgtcttg
     1381 ccggcgcgaa tcgcctcacc gaccgcgatc gcctcgtact ggtagccggt cgtgttttcc
     1441 tccgggaagt cgaagacctc cggctcacca ttcaggggga agagcgtgat ctgcttcggt
     1501 tcccagaagc ggcgggagac ctcgatgcgt cccttctcgc cggcgacgac ggcgatgttg
     1561 ttcggggtcg cacggaagga gccggtgagc gacgcgacgg cgccggacgg atagccgaag
     1621 gagatcgatt cctgctcgtc gaccccggtc tcgccgatca cgaccgagct gctgatctgg
     1681 ttcggtgtgc cgagcaccat gaaggcgaac gagatcgggt agatgcagcc atcgaggagg
     1741 agtccgccgc caagatcctt gttgtagaga cggctggcgg gattgttgcc gccgagccag
     1801 ccgatcgagg cgctgacgat ctgcggcgtg ccgatcgcac cgtcggcgat caactcacgc
     1861 accttcacca tcgccgggcg gaagcgggtc cacatcgctt ccatcaggaa gaggttcttc
     1921 gcgcgggcgg tatcgatcat ttcccgcgcc tggcgggcat tcatcgcgaa gcccttctcg
     1981 cagagcaccg ccttgccgcc gttgagggag aggatcgtcg cctcgtggtg ccacgggtgg
     2041 ggagtcgcga cgtagatcgc gtcgacctcg ggatcggcgg cgagaagttc gtagctggga
     2101 tgacgattcg gaatcccgaa ttcatcgccg aacgcgtccg cggtctcctg gctacgcgag
     2161 ccgaccgccg tcacgacggc gccgggcacg gcagcctgca cgtcgtgcgt gaatttgtgg
     2221 gcgatcccgc ccgtcccgag aatgccccag cgaatggtgg agaggtcgct cacgaggtct
     2281 ggatcctttc acgcgcggcg gcgcgtcgtc tgacaagtcg aagtcgttgg tactgtgtca
     2341 cagatgcggt gcgacgaaaa gcggttgcgg taactcgcag tgccgggaac gtgggatcgg
     2401 cccacgggag gcgggacata gaagtcccgc cctacgcacg accgctgatt ccgagagcat
     2461 gcgcgggaac gcggctcgtc cgggctgaat ccgatcgccg tgctacgcgc cgtcgccgac
     2521 gaggtcgtct acaccgcggt cgtgcgtggg ggctgtggac tgcggagacc aaagaccggc
//
LOCUS       DASTMD010000254         4568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_560365, whole genome shotgun sequence.
ACCESSION   DASTMD010000254 DASTMD010000000
VERSION     DASTMD010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560365"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1790
                     /locus_tag="VFQ54_03795"
     CDS             <1..1790
                     /locus_tag="VFQ54_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0164141.1"
                     /translation="TKIDGSNVATLGPAWRIDLKDAAGEPAAVTGTPIVVGDLVYIQD
                     MNSNVTAYDRASGELKWTKTYNFPCNGPNGVTYGYGNLFFGLGQSAEVAAIDAKTGDD
                     VWRVKLSNMKEEGIRMSPVAYGGVVYISIVPLSTNNNPGSRGILHALDAQTGNTIWYF
                     DLSEDNLWGNARKNLGAGLWYAPNFDEKGNLYFGNGNAAPWPGDKEFPAGSSRKNANL
                     YASTAMSIDPTDGTVRWYFQPKPFDLFDLDYQIAPVIVQANINGADTKLAICSGKTGD
                     VVAMNADTGDKIWWTKVGKHKNDQLQQLTDQFIQVFPGGNGGVLTPPACADGVFYCVV
                     TNRPSWVNATGSESHPEEPWEGEVIAIDVETGNIIWDIHVPTFPTGSIIVANDQVITA
                     GLDGLVRAFNRKDGSQTWNFQLKAGINAPVAIAGDELYVAAGTQYRPSPDQITEEVAA
                     DATYELISFKLGVGGGVVPEATAPMNQAPVEDATAAATAATDDVAPTTPEADDDGELK
                     LIVEMGDLYFKPRAIAIPANNDVKITLRNVGALQHDFVIDSPAVDSGMVASGTSGQVV
                     LNLPAGTYTFYCSVEGHADAGMVGTITAE"
     gene            complement(1923..3053)
                     /locus_tag="VFQ54_03800"
     CDS             complement(1923..3053)
                     /locus_tag="VFQ54_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU0164142.1"
                     /translation="MATEPRSNLIPVFDGHNDTLLDLAVTGRSFYERSDVGHIDRVRA
                     EEGGLVGGFFATWVPSIGRSEESDAKVREMLGSGLDGVPLVTEEGWLSLAYAQQYTHA
                     AVARLLRLEAGEGAIALVNKDLDYEIPADVLSVVLSAADLEENIENGDFSAIMHFEGA
                     EMIDENFYALETYYSAGLRSLGLVWSRPNRFGHGVPFGFPSSPDTGPGLTDAGKALVQ
                     ACNELGIMLDLSHLNEKGFWDVAAISDAPLVATHSNVHAICASSRNLTDKQLDAIAES
                     DGIVGLNFNVGFLRPDGGRDGTMPLSVMADHVDYMVERIGIDRVALGSDFDGAQMPDE
                     LKDVAGLPKLIDVLRTRGYDDDALRKIGYENWLRVLELTWGM"
     gene            complement(3222..4355)
                     /locus_tag="VFQ54_03805"
     CDS             complement(3222..4355)
                     /locus_tag="VFQ54_03805"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU0164143.1"
                     /translation="MSEPTTPTELIPIIDGHNDTLLSLVVDERSFYERSEKGHIDRVR
                     AAEGGLAGGFFAIWVPSIDFDPQLQAKASVLVGNELDGAKILNDNGWLSLPFSQQYTH
                     QAVARLLRIEAGEGAELAFNSELGFEIPVDPLTIVLSAAELEENVENGDFSAIIHFEG
                     AEMIDENLYALETFHAAGLRSLGIVWSRPNRFGHGVPFEFPSSPDTGPGLTDLGRELV
                     QGCNALGIMIDLSHLNEKGFWDVAGISDAPLVATHSNAHAISAGSRNLTDRQLDAIKE
                     SDGLVGLNFHVGFLRPDGAWDANTPLTVMADHIDYLVDKLGIDRVALGSDFDGATMPA
                     ELKDAAGLPKLIDTLRARGYDDESLRKIGYRNWVRVLELTWGA"
BASE COUNT          890 a         1464 c         1425 g          789 t
ORIGIN      
        1 agacgaagat cgacggctcc aatgtcgcca cgttgggccc ggcctggcga atcgatctca
       61 aggatgccgc gggtgagccc gccgccgtga ccggcacgcc gatcgtcgtc ggcgatctcg
      121 tctacatcca ggacatgaac agcaacgtca ccgcctatga ccgcgcgtcg ggcgaactga
      181 agtggacgaa gacctacaac ttcccatgca acgggccgaa cggcgtcacc tatggctacg
      241 gcaacctctt cttcgggctc ggtcaatcgg ctgaagttgc cgcgatcgac gccaagaccg
      301 gcgacgatgt ctggcgcgtc aagctcagca acatgaagga agaaggcatc cggatgtcgc
      361 cggtcgccta cggtggcgtc gtctacatct ccattgttcc cctctcgacc aacaacaatc
      421 cgggctcccg cgggattctc catgccctcg atgcccagac cggcaatacg atctggtact
      481 tcgatctctc ggaagataac ctctggggta atgcccgcaa gaacctcggc gccggtctct
      541 ggtacgcgcc gaacttcgat gagaagggca acctttactt cggcaacggc aatgccgcac
      601 cgtggccggg tgacaaggaa ttccccgcag gttcgagccg caagaatgcg aacctgtacg
      661 ccagcactgc gatgtcgatc gatccgacgg acggtacggt gcgctggtat ttccagccga
      721 agccgttcga cctcttcgac ctcgattacc agatcgcgcc ggtcatcgtg caggccaaca
      781 tcaatggtgc ggataccaag ctcgccatct gctcgggcaa gaccggcgat gtggttgcaa
      841 tgaacgcgga caccggcgac aagatctggt ggacgaaggt cggcaagcac aagaacgatc
      901 agctgcagca gctgaccgat cagttcatcc aggtcttccc gggtggcaat ggcggcgtcc
      961 tcaccccacc ggcgtgtgcg gacggtgtct tctactgcgt ggtgaccaat cgcccgagct
     1021 gggtgaatgc gaccggttcg gagtcacatc cggaagaacc gtgggaaggc gaggtcatcg
     1081 cgatcgatgt cgagaccggc aacatcatct gggatatcca cgttccgacc ttcccgacgg
     1141 gttcgatcat cgttgccaac gaccaggtga tcaccgccgg gctcgacgga ctcgttcgcg
     1201 ccttcaatcg caaggatgga tcgcagacct ggaacttcca gctcaaggcg ggcatcaacg
     1261 ctccggtcgc gatcgcgggt gacgagctct acgtcgccgc cggcacgcag taccgcccgt
     1321 cgccggacca gatcacggaa gaggtcgcgg cggacgcgac gtacgagttg atctccttca
     1381 agctcggcgt tggcggcgga gtcgtcccgg aggcgaccgc gccgatgaac caggcaccgg
     1441 tcgaggacgc cacggccgcc gcgactgcgg cgacggatga cgtcgccccc acgacaccgg
     1501 aagcggacga cgatggcgaa ctgaagctga tcgtcgagat gggcgatctc tacttcaagc
     1561 cgagggcaat cgcgatcccg gcgaacaacg atgtgaagat cacgctgcgc aacgtcggcg
     1621 ccctgcagca cgacttcgtc atcgacagcc cggccgtcga ttccggcatg gtcgcgtccg
     1681 gcacgagcgg gcaggtcgtg ctcaaccttc cggcgggcac ctacaccttc tactgctccg
     1741 tcgagggcca cgcggacgcg ggcatggtcg gaacgatcac cgccgagtag ccaacgtcag
     1801 aggtctcacg gcaacgaaag aggggctggc cattgtggcc agcccctccc tgtttgtgat
     1861 cgctgcgaat aaaaccgcgt cggcgccagg ccgaccccct tacggatttg cggccacgcg
     1921 aactacatgc cccaggtcaa ctcaagaacg cgcagccagt tctcgtaacc gatcttgcgt
     1981 agcgcgtcat cgtcatagcc acgcgtacga aggacatcga tcagcttcgg caatcccgcg
     2041 acgtccttga gctcgtccgg catctgggcg ccatcgaaat cggagccaag cgcgacgcgg
     2101 tcgatcccga tccgctcgac catgtagtcg acgtgatcgg ccatcaccga cagcggcatc
     2161 gttccgtcgc ggccgccatc cggacgcagg aagccgacat tgaagttgag gccgacgatc
     2221 ccgtcggact cggcaatcgc gtccagctgc ttgtcggtca ggttgcggga cgaggcgcag
     2281 atcgcatgca cgttggaatg ggtcgcgacg agcggcgcat ccgagatcgc cgcgacatcc
     2341 cagaacccct tctcgttgag gtgggaaaga tcgagcatga tcccgagctc attgcaggcc
     2401 tgcaccagcg ccttgccggc gtcggtaaga cccggtcccg tatccgggct ggacgggaag
     2461 ccgaacggca cgccgtgccc gaagcggttc ggacgcgacc agaccagacc cagcgagcgg
     2521 agtccggccg agtaataggt ctcgagcgcg tagaagttct cgtcgatcat ctcggcaccc
     2581 tcgaagtgca tgatcgccga gaagtcgcca ttctcgatgt tctcttcgag atcagccgcc
     2641 gagaggacga cgctcagcac gtccgcggga atctcgtagt cgagatcctt gttgacgagc
     2701 gcgatcgcgc cttcgcctgc ctcgagccga agcaaccggg ccaccgcggc atgggtgtat
     2761 tgctgcgcgt aggcgagcga gagccagcct tcctccgtca cgagaggtac gccgtcgagt
     2821 ccgctgccga gcatctcgcg gaccttggcg tcgctctcct cactccgccc gatgctcggg
     2881 acccacgtcg cgaagaatcc accgacgagg ccaccctctt cggcgcggac gcgatcgatg
     2941 tgcccgacgt cactccgctc gtagaaggag cgaccggtga cggcaagatc gaggagcgtg
     3001 tcgttgtgcc catcgaaaac ggggatcagg ttggatctcg gttctgtcgc cacgtaaatc
     3061 tcttcttcct gcatgacagc gggggccgat cgaaagacca cgacgcgatc tcctcatggt
     3121 ggcgatgcgc cgtcgtccta cggccacacg cgacggatac accggatgta tccgcctatg
     3181 atgcccgatc gcgccccgaa cgcaactccc ggcgccgatc gctacgcgcc ccacgtcaat
     3241 tcgagaaccc gcacccagtt cctgtatccg atcttgcgca gcgactcgtc gtcgtagcca
     3301 cgagcacgca gggtgtcgat cagctttggc aatccggcgg catccttgag ttcggccggc
     3361 atcgtcgcgc cgtcgaagtc ggaaccgagc gcgacgcgat cgatgccgag cttgtcgacg
     3421 agatagtcga tgtggtccgc catcaccgtc agcggcgtgt tcgcatccca ggcgccatcc
     3481 gggcgcagga agccgacgtg gaagttgagc ccgacgagac catccgactc cttgatcgca
     3541 tcgagctgcc tgtcggtcag gttgcggcta cccgccgaga tcgcgtgggc gttggaatgg
     3601 gtcgcgacga gcggcgcgtc ggaaatcccg gcgacatccc agaatccctt ctcgttgagg
     3661 tgggagaggt cgatcatgat cccgagcgcg ttgcagccct ggacgagttc ccgcccgaga
     3721 tcggtcagcc ccggaccggt gtccgggctg gacggaaact cgaagggaac gccgtgcccg
     3781 aagcggttcg gacgcgacca gacgataccg agcgagcgaa gtcccgcggc atggaaggtc
     3841 tccagcgcgt agagattctc gtcgatcatc tcggcgcctt caaagtgaat gatcgccgag
     3901 aagtcgccat tctcgacgtt ctcctccagt tccgccgcgg agagcacgat cgtcagcgga
     3961 tcgaccggaa tctcgaagcc gagttcggaa ttgaacgcga gttccgcgcc ctccccggcc
     4021 tcgattcgca gcagccgggc aacggcctga tgggtgtact gctgggagaa tggcagcgag
     4081 agccaaccgt tgtcgttgag gatcttcgca ccatcgagtt cgttaccgac gaggacgctt
     4141 gccttcgcct gcagctgggg atcgaaatcg atcgacggca cccagatcgc gaagaagccg
     4201 ccggcaagcc caccctcggc ggcacgcacg cggtcgatgt gccccttctc gcttcgctcg
     4261 tagaaggagc gctcgtcgac gaccaggctc aggagcgtgt cgttatgacc gtcgatgatg
     4321 ggaatcagct cggtgggtgt cgtcggctcg ctcatctgaa tgcatctctt tccgtggaca
     4381 gcggtcgcta cagaactacg cggacatctt cgccgatccg tggtcggtgg caagcgtcag
     4441 tcgcgcaagg tgggcgttcg cgaactgggc tgtgcgtcca atgactggga ctgaacggac
     4501 gacggttggc ggcgagttgt gttctggcat gacggcacga acacgcaaag agggtgagca
     4561 atgcgtgc
//
LOCUS       DASTMD010000255         4306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2434694, whole genome shotgun sequence.
ACCESSION   DASTMD010000255 DASTMD010000000
VERSION     DASTMD010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2434694"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFQ54_03810"
     CDS             complement(<1..656)
                     /locus_tag="VFQ54_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164144.1"
                     /translation="MPSSEIADHIANVSVIDTHSHLPGDYAWEGPGAPDILSDLFGWY
                     SSSDLIVAGASLDAVARLQDSSDEDLEGRFAGVADAWSMARFTGYGEAVRIAARDLYE
                     IDEVSAATIRSGQKRLKALQREGGCIALLRDRAKLDHVQTDLGLDVIDLPRTSAAFFL
                     RDLSLRRFAIGAIDDPVIEEATGVTIRNLATLEQSMEALFARCAPLSIAVKSQHAYVR
                     T"
     gene            complement(841..1404)
                     /locus_tag="VFQ54_03815"
     CDS             complement(841..1404)
                     /locus_tag="VFQ54_03815"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU0164145.1"
                     /translation="MAGFIPEANPVFRGNAGQRLMIVGQAPGAKGHELGIPWAGQSGV
                     MLRGWMAQAGFPEEAFLETWYLTSITKCFPGKSTGSNGDRSPSAAEIRLCHDHLAREI
                     DLVQPELIVTLGRLAATSLIPGARKLSLTELVGSRRVANLGYGEIPILPLPHPSGVSR
                     WHNDPANRTLVDRALASIGVERVRLGM"
     gene            1548..2318
                     /locus_tag="VFQ54_03820"
     CDS             1548..2318
                     /locus_tag="VFQ54_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEU0164146.1"
                     /translation="MEETAVKPTSPATSDHTHQQLPRTNAAVNPAALGSPYFLVIVAL
                     YVTCLIMANILAVKILHIGPWVADAGTMTFPVAYIVGDILTEVYGYSIARRVIWLGFI
                     CNLLAVGMIQIAGAMPADSSWDGGDAYNRIFGSTPRILFASACAYIVGEFVNSYVLAR
                     MKIWTNGRFLWTRTIGSTIVGEGLDSVIFVTVAFAGTMSGGLVWQMVYTNWILKTVYE
                     TIFTPVTYLVINKLKQIEGVDKFDRGTNFNPLAVGSVS"
     gene            2547..3140
                     /locus_tag="VFQ54_03825"
     CDS             2547..3140
                     /locus_tag="VFQ54_03825"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEU0164147.1"
                     /translation="MLSHGSDHLQMHATRSSRRKFLATGFAATLLAGSLQAAPRSMLA
                     ASQATPAASPVATPAVVAGTEESPASLNQLVNVESFTVDVISCTSVSAALPGGGDAPA
                     TGNVYAVIRLKVTNVTDSSDYVGRGDFAASDPANDDEVDSRNPENADRPFKGGDLDPG
                     EFVEGDLVFEIPEALDKIKLRFRPSLFGDSGYWLLPV"
     gene            complement(3341..3856)
                     /locus_tag="VFQ54_03830"
     CDS             complement(3341..3856)
                     /locus_tag="VFQ54_03830"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HEU0164148.1"
                     /translation="MSANQTDTRTGEPAIIRGRFVANPPPGTVVFLFGIRINSWSQIR
                     HWLPVVRSLRRMIREQEARPDSGLLWSTRWRDGREITVLQYWRDMDALMGYAQDKSFT
                     HAGAWKTFNHGVGDSGIIGLWHEAYTIDAESPRGLHTIYRDIPERGLAAATERVDAEQ
                     HALRRFARERG"
     gene            complement(3889..>4306)
                     /locus_tag="VFQ54_03835"
     CDS             complement(3889..>4306)
                     /locus_tag="VFQ54_03835"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HEU0164149.1"
                     /translation="MRINQLWNVRKWGPVAAAMGPMLAELSREPSLGLLGFKSWVSWR
                     SVIVQQYWTNSEQLMAYASSRDANHLPAWKRFNARVGHNNPAVGIWHEAYVVEPETTH
                     IVYVNMPPFGMGKATSLRPANEMGSQPLRRPATVSE"
BASE COUNT          742 a         1362 c         1382 g          820 t
ORIGIN      
        1 gtgcggacgt aggcgtgctg ggacttgacc gcgatggaga gcggagcaca tcgggcaaac
       61 agcgcctcca tcgactgctc cagcgtggcg agattccgga tcgtcacacc ggtcgcttct
      121 tcgatgaccg gatcgtcgat cgcgccgatg gcgaaccggc gcaacgagag gtcgcggagg
      181 aagaaggccg cggacgttcg gggaaggtcg atcacgtcga gccccagatc cgtctggaca
      241 tgatcgagct tcgcacgatc gcgcaacagc gcgatacacc caccttcgcg ctgtagcgcc
      301 ttcaggcgct tctgccccga tcgaatggtc gccgcgctca cctcgtcgat ttcatacagg
      361 tcacgagcgg cgatgcgcac cgcttcgccg tatccggtga accgggccat ggaccaggca
      421 tctgccacgc cggcgaagcg gccctcaaga tcctcgtcgc tgctgtcctg aaggcgggca
      481 acggcgtcga gcgaggcgcc ggcgacgatc aggtccgacg agctgtacca gccgaagagg
      541 tcgctgagaa tgtcgggcgc gcctggtccc tcccaggcat aatcgcccgg cagatgggaa
      601 tgcgtatcga tcactgagac gttggcaatg tggtcggcaa tctcgctgct cggcatcggt
      661 cctccgttgc ttcgctgctc tgtccccagg ccgcagcagt aatccggcaa tacgctcacc
      721 cgaaacctgg ccgtccgctc cgccagatcc gcgtggagag acgagaattc gttcggcagg
      781 cggtgccttc ccggattcgc gctcccatat ctcgcggcga ccccgttcgg tccaggagca
      841 ttacataccc aggcggacgc gttccacgcc gatcgaggcg agggcgcggt cgaccagcgt
      901 acgattggcc ggatcgttgt gccagcggga gacaccggat gggtggggca acggcaagat
      961 cggaatctca ccgtagccga ggttggcgac gcggcgggat ccgacgagct cggtcaggga
     1021 gagcttcctg gcacccggga tcagggacgt cgccgcgagg cgcccaaggg tgacgatcag
     1081 ttccggctgg accagatcga tctccctcgc gagatgatcg tggcagagac ggatctccgc
     1141 ggccgacggc gagcggtcgc cgttgcttcc agtggacttg ccggggaagc atttcgtgat
     1201 gctcgtcagg taccaggttt cgaggaacgc ctcctccggg aatcctgcct gcgccatcca
     1261 gccgcgcaac atcacgccgg actgcccggc ccacggaatc ccgagctcat gacccttcgc
     1321 gcccggcgcc tggccgacga tcatcaggcg ctgacccgcg tttccgcgga agaccggatt
     1381 cgcctccgga atgaatcctg ccacgacaca gcgcgtgcac gcgcgaatct catcctgata
     1441 ctggctcaat atggatgacg ggtccgattg tgtgattgcc tcgttcatgg gtaccattcg
     1501 ggtcgcggag gacgatcgag ttcctcacac gggagtcatg catctttatg gaagaaaccg
     1561 ccgtcaagcc aacctcgccc gccacgagcg atcacaccca ccagcaattg ccgcggacaa
     1621 atgcggcggt caatccggcc gcgctgggct cgccctattt tctcgtgatc gtcgcgctct
     1681 atgtgacgtg cctgatcatg gcgaatatcc tggcggtgaa gatccttcac atcggcccgt
     1741 gggttgcgga cgccgggacg atgaccttcc cggtcgccta tatcgtcggc gatatcctga
     1801 ccgaggtcta tggctactcc atcgcgcgtc gtgtgatctg gctcggcttc atctgcaatc
     1861 tgctcgccgt cgggatgatc cagattgcgg gcgcgatgcc ggccgactcg agctgggacg
     1921 gtggagacgc ctacaatcgc atcttcggct cgacgccgcg catcctcttt gcctcggcct
     1981 gcgcctacat cgtcggcgag ttcgtcaact cctacgtcct ggcgcggatg aagatctgga
     2041 ccaacggccg cttcctctgg acgcggacga tcggctcgac gatcgtcggt gagggtctgg
     2101 attccgtcat cttcgtcacc gtcgccttcg cgggaacgat gagcggtggt ctcgtctggc
     2161 agatggtcta cacgaactgg atcctgaaga cggtgtatga gacgatcttc accccggtga
     2221 cctacctcgt gatcaacaag ctgaagcaga tcgagggggt cgacaagttc gatcggggga
     2281 ccaatttcaa cccactcgcg gttgggtcgg tctcctgatc ctgggtcata tctgatgtct
     2341 cgctgaatac gaatggaaac cgcgacgatc atctccgatc gtcgcggttt ccatgccggc
     2401 gattgccggt ttccccccat ccgaaccccg atagcggtca gcaagaatat ggacagggcc
     2461 tgtttacgcg agattgatga agtcttgcga atcagtgata cccttcttcg taatggatgc
     2521 ggcttgaatg tacggtggag ggttccatgc tgtcccacgg gtccgatcat cttcaaatgc
     2581 acgccacgcg ctcgagccgg cggaagttcc tggcgaccgg cttcgccgcg acgctgctgg
     2641 cgggatcgct ccaggccgcg ccccggagca tgctcgcggc atcgcaggcg actcccgcgg
     2701 cttcaccggt ggcgacaccg gccgttgttg ctggtacgga ggagagcccc gcgagcctca
     2761 accagttggt caacgtcgag tcgttcacgg tcgacgtgat ctcgtgcacg tcagtgagtg
     2821 ccgcgctgcc cggtggcggc gacgctccgg caacgggcaa tgtctacgcg gtgattcgcc
     2881 tgaaggtgac caacgtaacc gattccagcg attacgttgg ccggggcgac ttcgcggcgt
     2941 ccgatcccgc gaatgacgac gaggtggatt cccgcaatcc ggagaatgcc gacaggccgt
     3001 tcaagggtgg cgatctcgac cccggcgagt ttgtcgaagg ggatctggtc ttcgagatcc
     3061 cggaggccct ggacaagatc aagcttcgat tccgaccgtc actcttcggg gattcgggct
     3121 actggctcct gccagtctag cgcggcgatc gagcccgttg ccgcggagag cgcgatccgc
     3181 ggacgaacgc gaggcggtcc ggcaaccgcg gtcctgccct gtgaacgacc gccgcttcga
     3241 cggccgaatc cggaaccgtc gacaggtttg gtggcactgg ggttggtagg gcagggtttc
     3301 ataccccgcc gcaaagcgga cctgactcca ggcggaggcg ttacccgcgt tcgcgggcaa
     3361 agcgacggag cgcatgctgc tcggcatcga cgcgttccgt cgccgccgcg aggccgcgct
     3421 ccgggatgtc gcggtagatc gtgtgcaggc cgcgcggtga ttcggcatcg atcgtgtatg
     3481 cctcgtgcca gagaccgatg attcccgaat cgccgacgcc gtggttgaac gtcttccacg
     3541 ctcccgcatg cgtgaatgac ttgtcctgtg cgtagcccat cagcgcatcc atgtctcgcc
     3601 agtactggag gacggtgatc tcgcgcccgt cgcgccagcg cgtcgaccag agcagtccgg
     3661 aatccggacg cgcctcctgt tcccgaatca tccgccgcag cgatcggacg accggtagcc
     3721 agtgccggat ctggctccag ctgttgatcc ggatcccgaa caggaagacc accgtccccg
     3781 ggggcgggtt cgccacgaat cttccccgaa tgattgctgg ttcgccggtt cgcgtgtcgg
     3841 tctggttcgc gctcatcgtg tgccttcatc ctgatcccga tatcgggatc attctgacac
     3901 ggtcgcgggg cggcggagcg gttgcgatcc catttcgttc gcgggccgga gcgaggtcgc
     3961 cttgcccatc ccgaagggcg gcatgttgac gtagacgatg tgcgtcgtct cgggctcgac
     4021 gacgtaggct tcgtgccaga tgccgacggc ggggttgttg tgaccgacgc gcgcattgaa
     4081 acgcttccag gcggggaggt gattggcgtc acgggaagaa gcgtaggcca tgagttgctc
     4141 gctgttcgtc cagtattgct ggacgatgac ggatcgccag ctgacccagc tcttgaatcc
     4201 gagcaggccg agcgatggtt cccggctgag ttcggcgagc atcgggccca tcgccgcggc
     4261 gactggcccc cacttgcgca cgttccacag ctggttgatc cgcatc
//
LOCUS       DASTMD010000256         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3148792, whole genome shotgun sequence.
ACCESSION   DASTMD010000256 DASTMD010000000
VERSION     DASTMD010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3148792"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFQ54_03840"
     CDS             complement(<1..258)
                     /locus_tag="VFQ54_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164150.1"
                     /translation="MSLAMVLALGLGLFGPQFIARAQDTPVQGGTYRTAISEEPDQLD
                     PAKTNELLASEIMNNIYDELVYIGSDGLPHPWIAESWTISED"
     gene            complement(948..>2135)
                     /locus_tag="VFQ54_03845"
     CDS             complement(948..>2135)
                     /locus_tag="VFQ54_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0164151.1"
                     /translation="PARNSVVSSTHPGVVDLQGEYFVGNVVRMGIVGAGTIAQRGILP
                     HLSQADVQDRVILQAVCDPVPGRAEAAAERFGIRKGFQEYADLLANGDVDAVSIASPI
                     GFHFEQGKLALQAGKHVHFNKTICTTVAEANELIALAQANDLRIVASPGEVLRPQVQA
                     VKKAIADGKIGKLIWAAAGASFGTYHLDEDERKGDDVLTNLDPSWYFRKPGGGPLYDM
                     TVYALHGLTTVLGPAKRVTAMSGVRIKEREFKGNLVPTDADDNTGILIDFGDALFALG
                     YGTAAGAITQGFGGTYFGTTGKIERYTINGEPIDYPEKEIADQAPPGDGPQWTLPHVL
                     GEHRNIMEQHVFEDIMQLVDWINEGKPSLVTAEHARHVVDIIESAYRAAETGQTQDLT
                     TSF"
BASE COUNT          376 a          691 c          652 g          416 t
ORIGIN      
        1 gtcctcactg atcgtccatg actccgcgat ccacggatgc gggagaccgt ccgagccgat
       61 gtagaccagt tcgtcataga tgttgttcat gatctcgctg gcgagcagtt cgttggtctt
      121 cgccggatcg agctgatccg gctcttcgct gatcgcggtg cgataggtgc cgccctgaac
      181 cggcgtatcc tgggcgcggg cgatgaactg cggcccgaac aatcccaaac cgagcgccag
      241 gaccatcgcc agagacaaga ctctggcccg taatccgagt ttcatggatt ccccccggtg
      301 tgatgcttgc tacgtcagaa acacggaaca cgtctgcgag ttccggatat ggcgatgcgg
      361 tgccctggtc gcacccgacg ttgggtgctc gtcatgctcc cggacccggg agtcgcacgg
      421 tgccccttgc cagccaggcg aggcaccacc tcaacgaaac gcccccaacc cctcccgtct
      481 gtcgaatcca tgcagaatgc acaaatcgtc caggcaatct gacgacatac aacacatcgc
      541 gatttggaca atcctgtctt tcgcctgagc gtgatgatat gaggcgttac gaaaagggga
      601 aagcgttctc cagatggtca ccttcatcga ggtttagccc gtgtacaagc cgggccgcgc
      661 cggaatgtgc gcgatgccca ttccggtctt cgctgcatga atgtgtcgtg agagatattg
      721 ccagcacact tatgcaaaac gaacagtttc caacccaatg ggatcgggaa ctgttcgtcc
      781 gcagcgaggc gatctcaggt cgccattgtt tcggcggctc gtcgccgcgc cgacgatcgc
      841 ggcattcccc ggtacgggag aacggtaaac cactccagac cgaaagaccc cacggctcga
      901 aatcgagccg tggggtcttc gcggttctcg cttgggtgcg gagtcgatca gaacgaggtc
      961 gtcaggtcct gcgtctgtcc cgtctcggcg gcacggtacg ccgactcgat gatgtcgacg
     1021 acgtggcgcg cgtgctcggc ggtgaccagc gatggcttgc cttcgttgat ccagtcgacg
     1081 agctgcatga tgtcctcgaa gacgtgctgc tccatgatgt tgcggtgctc accaaggacg
     1141 tgcggcagcg tccactgcgg accgtcgccc ggaggcgcct gatcggcgat ctccttctcc
     1201 ggatagtcga tcggctcacc attgatcgtg tagcgctcga tcttgccggt ggtcccgaag
     1261 taggtgccgc cgaatccctg cgtgatcgct cccgccgccg tcccgtagcc cagcgcgaag
     1321 agggcatcgc cgaagtcgat caggatgccg gtgttgtcgt cggcgtccgt cggcacgagg
     1381 ttgcccttga actcgcgctc cttgatccgg acgccggaca tcgcggtcac ccgcttcgcc
     1441 ggtccgagca ccgtcgtcag accgtgcagc gcgtagacgg tcatgtcgta gagcgggccg
     1501 ccgcccggct tgcggaagta ccacgagggg tcgaggttgg tcaggacgtc gtcgcccttc
     1561 cgctcgtcct catcgaggtg ataggtgccg aaagacgctc cggcggcggc ccagatcagc
     1621 ttgccgatct tgccgtcggc aatcgccttc ttcaccgcct gcacctgtgg tcggagcact
     1681 tcgcccggcg acgccacgat ccggagatcg ttcgcctgcg ccagcgcgat caactcgttc
     1741 gcctcggcga cggtcgtgca tatcgtcttg ttgaagtgga cgtgcttgcc ggcctgaagc
     1801 gcgagcttgc cctgctcgaa gtggaacccg atcggcgacg cgatcgagac ggcgtcgaca
     1861 tcgccgttcg caagcaggtc cgcgtactcc tggaatccct tgcggatgcc gaagcgctcc
     1921 gccgcggctt ccgctcgacc cggaaccgga tcgcagacag cctgcaggat cactcgatcc
     1981 tgcacgtccg cctgactcag atgaggcagg attccgcgct gggcgatggt tcccgcgccg
     2041 acgattccca tgcgaacgac attacccacg aagtactctc cttgcaggtc gaccaccccc
     2101 ggatgggtgg acgaaacgac gctgttccgc gccgg
//
LOCUS       DASTMD010000257         1526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1631760, whole genome shotgun sequence.
ACCESSION   DASTMD010000257 DASTMD010000000
VERSION     DASTMD010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631760"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..>1526
                     /locus_tag="VFQ54_03850"
     CDS             87..>1526
                     /locus_tag="VFQ54_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164152.1"
                     /translation="MVRAAGAQDATPGASPAASPAASPVAYNGPVGTLSPDRAKVNQD
                     INEHFKFEEAGSEGGDIIFVETTDISTVNSILVSDTYSSWITGFLSDFLVGASPIDGT
                     WTPGLADSWEIAADGVTYTFHLHEGVKWHDGEPFTADDVVFSFDATVDEKSLSVRRGD
                     VLSVLKSYKKIDDLTVEFVATEPVATFIDKTVGQVGIVPKHIWGDVPVADWGSDPGST
                     GTDPKRVIGNGAFKFVEWVQNDHVTLEKNPDYYDAANTPVHADHFIYRVIADANAAIQ
                     SIKTGESDIVEVPFAQAQALGQSNPELAISAFDTFSFNYYQLNEDPAKSPLFHDARVR
                     QALHYGLDRDLIAQTVYDGYAVRADGTQPILSQAYKPDQINTIYELDVDKAKQLLADA
                     GWTDTDGDGIVDKDGVKMSFGLEYAEAVATYVQQIPYMQQAWKEIGVEMIPTSTPFPV
                     LLDHNDARSFQAQVLGFSWSVDPDQGAMFR"
BASE COUNT          296 a          519 c          441 g          270 t
ORIGIN      
        1 atcaactgcg caacggtgaa gtcagccgtc gcgggttcat tcgtcgtgct actgcgcttg
       61 gcctctccgc cgctgctgca ggatcgttgg tccgtgccgc gggcgcacag gatgccacgc
      121 caggagcgtc gcctgctgca agccccgccg ccagcccggt cgcctacaac ggtcccgttg
      181 gaacgctttc gccagaccgc gcgaaggtca atcaggatat caacgagcac ttcaagttcg
      241 aggaagccgg cagcgaaggc ggcgacatca tcttcgtcga aacgaccgac atctcgacgg
      301 tcaactcgat cctcgtctcc gacacctatt ccagctggat caccggattc ctcagcgact
      361 tcctcgtcgg agccagcccg atcgatggca cgtggacccc gggtctcgcc gactcctggg
      421 aaatcgccgc cgacggtgtc acctatacct tccatctcca tgagggtgtg aagtggcacg
      481 acggcgagcc gttcaccgcc gatgatgtcg tcttctcctt cgacgccacc gtcgacgaga
      541 agagcctcag cgttcgccgc ggagacgtcc tctccgtcct caagagctac aagaagatcg
      601 acgatctcac cgtggagttc gtcgccaccg agcccgtggc gaccttcatc gacaagaccg
      661 ttggtcaggt tggcatcgtg ccgaagcaca tctggggaga cgttcccgtc gcggactggg
      721 gttctgaccc cggatcgacc ggcacggatc ccaagcgagt catcgggaac ggcgccttca
      781 agttcgtcga gtgggtccag aacgaccacg tcacgctcga gaagaacccg gactactacg
      841 acgccgccaa tacccctgtc cacgccgatc acttcatcta tcgcgtgatc gccgatgcga
      901 acgccgccat ccagtcgatc aagaccggcg agtccgacat cgtcgaggtt cccttcgcgc
      961 aggcgcaggc cctcggccag tcgaacccgg agttggcgat cagcgcattc gatacgttct
     1021 ccttcaacta ctatcagttg aacgaggatc ccgcgaagtc cccgctcttc cacgatgcca
     1081 gggttcgcca ggcgttgcac tacggcctcg accgcgatct gatcgcccag accgtctatg
     1141 acggctacgc ggtgcgggct gacggcacgc agccgatcct ctcgcaggcg tacaagccgg
     1201 accagatcaa cacgatctac gagctcgatg tcgacaaggc gaagcagttg ctcgccgacg
     1261 ccggctggac cgacaccgat ggcgacggca tcgtcgacaa ggacggcgtg aagatgagct
     1321 tcgggctcga gtatgccgag gccgtcgcca cctacgtcca gcagatcccg tacatgcagc
     1381 aggcatggaa ggaaatcggc gtcgagatga tcccgacctc gacgccgttc ccggtcctcc
     1441 tggatcataa cgacgcccgc agcttccagg cgcaggtgct cggcttctcc tggagcgtcg
     1501 atcctgacca gggcgcgatg ttccgc
//
LOCUS       DASTMD010000258         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_112372, whole genome shotgun sequence.
ACCESSION   DASTMD010000258 DASTMD010000000
VERSION     DASTMD010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112372"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFQ54_03855"
     CDS             complement(<1..597)
                     /locus_tag="VFQ54_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164153.1"
                     /translation="MPHSRRSSIVTMIVLLGTVLGLAAPSGARAAPAPESIHPVMTAQ
                     NAPVGTTKTYDSVEASLVSRDPKTGSLVTGVCYELLDVLTNDPLSKIGCDENSDGQVD
                     FKGLKPTQYTVHQTQAAKGFPLTDDFSIIVIPEDAHQSFPIYQRKEQNDEQHRNVSIV
                     FSVPGNGDRLAGKGVCVEIVGATKPGCDDNGDGQVDFVD"
     gene            complement(905..2059)
                     /gene="msrA"
                     /locus_tag="VFQ54_03860"
     CDS             complement(905..2059)
                     /gene="msrA"
                     /locus_tag="VFQ54_03860"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU0164154.1"
                     /translation="MLFDRLFNKKSVMPSPEDALPGRTNPAFQISGVHAVLGTKMTPP
                     FPEGLEVAEFGLGCFWGAERLFWEIPGVWTTAVGYSGGYTPNPTYEEVCSGQTGQVET
                     VLVVFDPAKVTYEELVKEFFEAHDPTQGMRQGADVGTQYRSAIYFHSDAQREIAETAK
                     ASYTVALQAKGFPAVTTEIAANGPFYYAEEYHQQYLHKVPNGYCGLEGTGVSCAIPRS
                     QRAPVTEEEPVLAPVSGGSHEVDEAPVATSPAVDPGLKPVPSTEDEWRQALSPAEYSV
                     LRQAGTERPFTGPYNGVWDDGTYRCAACGNPLFDSTTKFDHGCGWPSFGEALPGAVEY
                     IEDRSHFMVRTEVQCARCHSHLGHVFNDGPKEYDGARFCMNSISIDLDRR"
     gene            2253..3056
                     /locus_tag="VFQ54_03865"
     CDS             2253..3056
                     /locus_tag="VFQ54_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEU0164155.1"
                     /translation="MIQLEQGRQAAIRDLDAIISRLHGLGNDDWNRPTPNAGWEVRHL
                     AAHLTESVQRLTDRLSTIVTRRLGHPIASDTAVDISPVNGDQTPNNLIVALTHRRNSV
                     RHVLIALEEEDLDEPIHDATIPSGQANGLGQLTACVFEFGIHRYDLDAALDEREAGLP
                     REAIIAADDLYASRMVEIATTEGKRPLAPIAYHFDGALIDRWLTWNGHEWSENELQDI
                     PVVHVQGDESAIALFICGRIKATDHRLGVRGDRDVAATFKEYVPGPFTI"
     gene            complement(3053..>3553)
                     /locus_tag="VFQ54_03870"
     CDS             complement(3053..>3553)
                     /locus_tag="VFQ54_03870"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0164156.1"
                     /translation="LVAAVVAAGIAWASQSDDRAAPRMAPDFSVQLMSSTSAWKLSNE
                     RGRVVVLNFWATWCDPCKREAPSLNEVATEMSDEGVAFIGIAPKSDNSSHIRSFVNSY
                     GLTYSIGQDSMGGDRLDGSITLNYGVYGYPTTVFIAPDGLISAVDIQPLSADEIENQI
                     ARAKNA"
BASE COUNT          638 a         1089 c         1164 g          662 t
ORIGIN      
        1 atcgacgaag tcgacctggc catcgccgtt gtcatcgcat cccggtttcg tcgcgccgac
       61 gatctcgacg cagacgccct tgcctgcaag gcggtcgcca ttgcccggca ccgagaagac
      121 gatcgagaca ttgcgatgct gctcgtcgtt ctgttccttg cgctggtaga tggggaacga
      181 ctggtgggca tcctcgggga tcacgatgat cgagaagtcg tcggttagcg ggaatccctt
      241 cgccgcctgg gtctggtgga cggtgtactg cgtgggtttc aggcccttga aatcgacctg
      301 gccgtcgctg ttctcgtcgc agccgatttt gctcagcggg tcgttcgtga ggacgtcgag
      361 caactcgtag cagacgccgg tgacgagcga tcccgtcttc ggatcgcggg agaccagcga
      421 ggcttcgacc gagtcgtagg tcttcgtcgt ccccaccggc gcgttctggg cggtcatcac
      481 cgggtggatg gattcaggtg ccggcgccgc gcgtgcgcct gatggcgcgg cgaggccaag
      541 gaccgttccc agcagcacga tcatggtgac gatcgatgat ctccgcgaat gaggcacgtg
      601 catgtgaatc ctccccgtgt tcacgaatga gcgtctggcg ctgatcccgg catcctgtcg
      661 attgggtgat gaaccggtcc cggccgggtc acgctcgggc ttggccgaat tgttctctcc
      721 cccttccgaa cgcttcggtc ctccgaacgg ttccgggaat tcgctgtgaa atccgttggg
      781 cgccgcaaac ccaccgcggg cccctgagtc gggcccgacc ggagaccaga tggagacaca
      841 cgaacgcccc cggagaagtc tctccggggg cgttgggacg acgtcaggat cgtcgggtgt
      901 gtgattagcg gcgatcgaga tcgatcgaga tgctgttcat gcagaagcgg gcgccgtcgt
      961 actccttcgg gccgtcgttg aagacgtgac cgaggtggga gtggcagcgc gcgcactgga
     1021 cttccgtgcg gaccatgaag tgcgagcgat cctcgatgta ctcgacggcg ccgggaagcg
     1081 cctcgccgaa gctcggccag ccgcagccgt gatcgaactt cgtcgtgctg tcgaagagcg
     1141 ggttgccgca ggcggcgcag cgataggttc cgtcgtccca gacaccgttg tacgggccgg
     1201 tgaaggggcg ctcggttcct gcctggcgca ggacgctgta ctcagccggg ctgagcgcct
     1261 ggcgccactc gtcctcggtg gacgggaccg gcttcagacc gggatcgacc gcggggctgg
     1321 tcgcaaccgg cgcctcatcg acctcatggc tgccaccact gaccggcgcc agcactggct
     1381 cctcctcggt gacgggagca cgctgcgatc gcgggatcgc gcagctgacg cccgttccct
     1441 cgagaccgca gtagccgttc ggcaccttgt gcaggtactg ctggtgatac tcctcggcgt
     1501 agtagaaggg accgttggcg gcgatctcgg tcgtcacggc cgggaaaccc ttcgcctgca
     1561 gcgcgacggt gtaggaggcc ttcgcggtct cggcgatctc gcgctgcgcg tcggagtgga
     1621 aatagatcgc ggaacgatac tgcgtgccga catccgcgcc ctggcgcatg ccctgggtcg
     1681 gatcgtgcgc ctcgaagaac tccttgacga gctcctcgta ggtcaccttc gcggggtcga
     1741 agacgacgag gacggtctcg acctgaccgg tctggccgga gcagacctcc tcgtaggtcg
     1801 gattcggggt ataaccgccg gaatatccga cagcggtcgt ccagacgccg ggaatctccc
     1861 agaagagacg ctcggcgccc cagaaacaac cgagaccgaa ctcggcgacc tcgagcccct
     1921 cggggaaggg cggcgtcatc ttcgtgccga ggacagcatg gacgccggaa atctggaagg
     1981 caggattcgt gcgaccggga agggcgtcct cgggggacgg catgacagac ttcttgttga
     2041 agaggcggtc aaacaacatg gatggtttct ccagcatgta ttgggggaaa cgtgtaccag
     2101 ggaggtcgat tcacgtccgg gggtggtgca agtctcactc gcttgtgtac cttacgaata
     2161 gtacttgacc tgtaaagggc attcatgtgc catggacgtc tgttcttgac cttcatctac
     2221 tatacgcatg acataaagaa aaggattgcg aaatgattca actcgaacag ggacgacagg
     2281 cggcgattcg ggatctcgat gcgatcatct ctcgcctgca cgggcttggc aacgacgact
     2341 ggaatcgccc gacgccgaat gcggggtggg aagttcgcca cctcgcggcg catctcacgg
     2401 aatcggttca gcgcctgacg gatcgtctct ccacgatcgt cacgcggcgc ctggggcacc
     2461 cgatcgcgag tgacaccgcg gtcgatatct ccccggtcaa cggcgaccag acgccgaaca
     2521 acctgatcgt cgccctgacg caccggcgga acagcgtccg ccacgtcctg atcgcgctcg
     2581 aggaagagga tctcgacgag ccgattcatg acgcgacgat cccgtcgggg caggcgaacg
     2641 gcctgggcca gctgacggcc tgcgtcttcg agttcggcat ccatcgctac gatctcgatg
     2701 ccgctctcga cgagcgggaa gcgggtcttc cccgtgaggc gatcatcgcg gccgacgacc
     2761 tctacgcgag ccggatggtg gagatcgcga cgacggaggg caagcgcccc ctggcgccga
     2821 tcgcctacca cttcgacggc gccctgatcg atcgctggct gacgtggaat ggccacgagt
     2881 ggagcgagaa cgaattgcag gatatccccg tcgtgcacgt gcagggggac gagtcggcga
     2941 ttgcgctctt catctgcggg cggatcaagg cgacggatca ccgcctcggc gtgcgtgggg
     3001 atcgcgatgt ggcagcgacc ttcaaggagt atgtccccgg cccgttcacg atctaggcgt
     3061 tcttcgccct cgcaatctga ttctcgatct catcggcgct cagcggttgt atatcgaccg
     3121 ccgagatcag cccgtctgga gcaatgaata ccgtcgttgg gtacccgtac acgccatagt
     3181 tcagcgttat cgatccatcg aggcggtcgc ctcccatgga gtcctgccca atggagtagg
     3241 tcagcccata ggagttgacg aacgagcgaa tgtgcgaact gttgtcgctc ttcggcgcga
     3301 ttcctatgaa ggcaacaccc tcatccgaca tttcggtcgc gacctcgttg agcgaggggg
     3361 cttcgcgctt gcacggatcg caccacgttg cccagaaatt gaggacgacg acacggcctc
     3421 gctcattgga gagcttccac gctgacgtgc tcgacatcag ctggaccgag aaatccggcg
     3481 ccatgcgtgg tgcggcgcgg tcatcggatt gagatgccca tgcaatgccc gcggcaacca
     3541 ctgcagccac gag
//
LOCUS       DASTMD010000259         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1184904, whole genome shotgun sequence.
ACCESSION   DASTMD010000259 DASTMD010000000
VERSION     DASTMD010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184904"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFQ54_03875"
     CDS             <1..279
                     /locus_tag="VFQ54_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164157.1"
                     /translation="FQRIAGIAHDPFASYFGTLDVGTGIEIGDIFVAPEELPLNEYLG
                     LARPPQSFQYVDVERAVSLLNLMRRVSGTGHHHVTYSSRQLALAAEAE"
     gene            419..781
                     /locus_tag="VFQ54_03880"
     CDS             419..781
                     /locus_tag="VFQ54_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0164158.1"
                     /translation="MTSYNGKICYLEIPSRNVQQSAEFYQTVFGWNIRTRGDGATAFD
                     DTTGEVSGSFELNRPAATEPGVLIYIMADSAAEAVRAVVAAGGEIVSPIDPDFREIIA
                     TFRDPSGNLFGIYQEPEH"
     gene            complement(811..1737)
                     /locus_tag="VFQ54_03885"
     CDS             complement(811..1737)
                     /locus_tag="VFQ54_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEU0164159.1"
                     /translation="MEQPTSTGSLDAWRVVIFTTIAGGIVYTLTDAVLQQFGHRVVGV
                     MTTPGPPARRSADYLSVVGAVRPGVDVLVSTHPSRWAAMLAPLRPDLIICCGFPYRIP
                     QAVVAMARLGAINVHPSLLPRHRGPWPLNWTFRSDDAVAGMTVHRITTAFDTGPILAQ
                     GSVPVEDEDDGDALMGKLAPLAVQLLAAALQRVAAGDPGDPQDERLATEAPFFEDAWY
                     RVDWSQPARTVHNQVRSWTGVSGGSHGALAEIDGELVVIARTRLLPVPETSVALAPGS
                     LIRRDDDGMIVQCGDGPIAVVAWHPVETANPD"
     gene            2043..2978
                     /locus_tag="VFQ54_03890"
     CDS             2043..2978
                     /locus_tag="VFQ54_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0164160.1"
                     /translation="MRTFTLPGSDIVAPNVVLGLMRIAQKSDSEIRELVRVAREAGIT
                     FFDHAAVYGPELHGCETRFAEALQLSPAERAEITIQTKAGIVREGPYFDFSYEHITES
                     VEASLKALGTDYIDILLLHRPDALVEPDDVARAFDDLHASGKVRAFGVSNHTPRQIDL
                     LKKSVRQPLIANQVQLSLTHAPIIAQGVASNMHGSDQSLTIDGGGVLDYCRLNDITIQ
                     AWSPFQAGFFTGVFFDSPDYPELNGVLDRLAAQYDVPPIAIATAWITRHPANIQVVLG
                     TTSPERVAAAAQGSEIPLTRAEWYELFRAAGYRVP"
BASE COUNT          646 a         1202 c         1059 g          605 t
ORIGIN      
        1 ttccagcgca ttgctggaat cgcacatgat ccatttgctt cttactttgg cacgcttgat
       61 gtcgggacag gcatcgagat tggtgacatt tttgtcgccc ccgaggaact cccactgaat
      121 gagtatctcg gcctcgcgcg acctccccag agctttcaat acgtcgatgt cgaaagagcc
      181 gtatccctcc ttaacttaat gcggcgtgtc tctggtactg ggcatcacca tgtcacctat
      241 tcatcccgac agctcgcgct tgccgcagag gcagaatagc caaagggggt tagatggtga
      301 tcgctggtcg cgcgaagcca gaccccgctc gccggcgcaa tgcaggagaa gcgccccgaa
      361 tgacggcagg ataatctcgc cgtaaggatt gtcttcattc agcgggaggg agccagacgt
      421 gacgtcatac aacgggaaga tctgctacct ggagattcca tcgcggaacg tccagcaatc
      481 ggccgagttc taccagaccg tcttcggctg gaacatccgc acgcgcggcg acggcgcgac
      541 cgcgttcgac gacaccaccg gtgaagtgag cggatccttc gaactgaatc gtccagccgc
      601 caccgaaccc ggcgtcctca tctacatcat ggccgatagc gcggccgagg cggtcagggc
      661 cgtcgttgcc gccggtggcg agatcgtctc cccgatcgac cccgattttc gcgagatcat
      721 cgccaccttc cgcgatccga gcggcaacct cttcggcatc taccaggaac ccgaacactg
      781 aatctgaaac gatgacgcac cggcgttcgc ctagtccgga ttcgccgttt ccaccgggtg
      841 ccacgcgacg accgcgatcg gcccgtcgcc gcactggacg atcatcccgt cgtcatcccg
      901 gcggatgagg ctgcccggcg cgagcgcgac cgatgtttcg ggaacgggca ggagccgcgt
      961 cctcgcgatc accaccagtt cgccgtcgat ctccgccagc gccccatgcg agccaccact
     1021 cacgccggtc cagctgcgga cctggttgtg caccgtacgc gccggctggc tccagtccac
     1081 ccgataccag gcatcctcga agaaaggcgc ctccgtcgcg aggcgctcgt cctgcggatc
     1141 gccggggtcg ccggccgcga cgcgctgcag cgcagcggcg aggagctgaa ccgcgagcgg
     1201 cgcgagcttg cccatcagcg catcaccgtc atcctcatct tcgaccggca cactcccctg
     1261 cgccagaatc ggtccggtgt cgaacgctgt cgtgatgcga tggacggtca tgcccgccac
     1321 cgcatcgtcg ctgcggaagg tccagttcaa cggccacggc ccgcgatgcc gcggcagaag
     1381 cgagggatgc acgttgatcg cccccagccg ggccatcgcc acgaccgcct gcggaatccg
     1441 atagggaaat ccgcagcaga tgatgagatc gggacgaagc ggcgccagca tcgccgccca
     1501 gcgtgacggg tgggtcgaga cgaggacatc gaccccgggg cgcaccgcac cgaccacgct
     1561 caggtaatcg gcactgcgcc gggcgggcgg tcctggcgtc gtcatcaccc ccaccacgcg
     1621 gtgaccgaac tgctgaagaa cggcatcggt cagcgtatag acgatcccgc ccgcgatcgt
     1681 cgtgaagatc acgacccgcc acgcatccag ggatccggtc gacgttggtt gctccattgt
     1741 tcctctcgat tcgctgcctg tgcgacgagt cgacaccgca cggcggcacg acgcgccgac
     1801 gctcgcagca ttgtcctgac aattcttgtt tgagacccaa cgtacccgct tgcattgtac
     1861 cgacctcccg ggcgtcaggc acgcgtcgag atcggcatgc ccgcgcgaca tcaggtcgcg
     1921 ggaggacagg tatcgagcgt gacatccggg ccgcaacgcg gccgtaacgc caggagagct
     1981 tgctacaatc cctgcggctc gctttggcca tcagcaccgt cgtagcagaa atggagacct
     2041 cgttgagaac gttcacgttg cccgggagcg atatcgtcgc ccccaacgtc gtgctcggcc
     2101 tgatgcgcat tgcccagaag agcgatagcg agattcgcga actcgtgcgg gtcgcccgcg
     2161 aggcggggat caccttcttc gaccatgccg ccgtctatgg cccggaactg cacggatgtg
     2221 agacgcgctt cgcggaagca cttcagctct cgcccgcgga acgcgccgag atcaccatcc
     2281 agacgaaagc cggcattgtc cgggaaggtc cctacttcga cttttcctac gagcacatca
     2341 ccgaatcggt agaggcctca ctgaaggcgc tcgggaccga ctacatcgac atcctgctgc
     2401 tgcaccgccc ggatgcgctc gtcgagcctg acgatgttgc gagggcgttc gacgacctcc
     2461 atgcgtccgg caaggtccgg gcattcggcg tctccaatca cacgccccgg cagatcgacc
     2521 tcctcaagaa gtcggtgcgg cagccgctga tcgccaacca ggtacagttg tcgttgacgc
     2581 acgcccccat cattgcccag ggcgtcgcct cgaacatgca tggctccgac cagtcgttga
     2641 cgatcgacgg cggcggcgtc ctcgactact gccggctgaa cgacatcacc atccaggcgt
     2701 ggtcaccgtt ccaggccggt ttcttcaccg gcgtcttctt cgactcgccg gattaccccg
     2761 agctgaacgg cgtgctcgat cgtctcgcgg cgcagtacga tgtgccgccg atcgcgatcg
     2821 ccacggcgtg gatcacccgc cacccggcga acatccaggt cgtgctcggc acgacatcgc
     2881 cggagcgggt cgccgccgcc gcgcaggggt cggagatccc gctgacgcgc gccgagtggt
     2941 acgaactgtt ccgcgccgca ggataccgcg ttccataggc gccatacgtc gcccgacagg
     3001 taacgcatgg aacgaagcgg cggtgggccc aaaggtccac cgccgcttct tctctgccgt
     3061 accgaacgac cagccatcgc ccgctggagc aagagtcgga agacgtgcct gccgatctac
     3121 cgtctggccg tcatcctgag cttgtagaag gatccccgcg cgaagcgcat caggcccatt
     3181 cgacgggctc agggtaaact ccgccggact ccgacgcggt cggttggcat gccacgtccg
     3241 gttgtgcacc tcggcaggca catcttttcg cagccgctct cgtggtctgt caccgatgtc
     3301 ttgccggttg gcgtcgtggg ccaacgatca tatgcggctt cgccgcatgg gccacacggg
     3361 gtggcccgct acgagatcac cgtggcaatt cagctgtgat gcaccgctag tcgacttcgg
     3421 cggttcgcgg gccgaagtcg gctgaccatt tctcctcgat ccggccaaag cgccatactc
     3481 cgagggcgat ggcccaggac acgaagaaca ga
//
LOCUS       DASTMD010000260         5144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2166653, whole genome shotgun sequence.
ACCESSION   DASTMD010000260 DASTMD010000000
VERSION     DASTMD010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166653"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(717..3794)
                     /locus_tag="VFQ54_03895"
     CDS             complement(717..3794)
                     /locus_tag="VFQ54_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0164161.1"
                     /translation="MALNRQPATTNGATPPPIPTLPTPPRSSSVSTKPDRSYRGAIET
                     ARVLEAELAARVQGEVRFDAVSRMLYATDASNYEIEPVGVVIPRSTDDVIAAIELATS
                     HSVPILTRGGGSSLAGQAVGAALVLDTSKYLDRLISVNPENRTVTVEPGINLDALNRQ
                     LKSHGLMFGPDPSSSNRATVGGVVSNNAAGAHSILYGMTQDNLQSARMQLADGSTVEL
                     GPGKMEDLLKRSQTDDATGRLLKKLLAFREERQELIARDFPPHWRRATGYSLNEFLKP
                     DDVFNPARLLASSEGTLGSLLQITLNLVPVPPITGLVMLSFDNLVRAMEVTPQILEVE
                     PSAIELMDRMLIGLTRQQPGYAKQISMIEGDPAAVLVVEFYGENERDLQQKADRLVDH
                     LRQRNITLMTDPQVVLDPKQQKDVWQVRKAGLGLLMSVRGDAKPIPAIEDVSVPVEHL
                     AAFVAAVEQMVARFHTTAAFYAHASAGCLHIRPLINLKTVEGVEAMRELAYEAAEIAH
                     SFGGVMSGEHGDGLQRSELNPIIFGPELYQTMRDFKHLFDPNDLMNPGKIVDAPPLVD
                     NLRYGPKYKPVPIRTNLDFSREGGFMAAVEMCNGAAVCRQTKSGTMCPSYMATKDEND
                     TTRGRANALRNALTGNILSPKDFGDKATYETLDLCLSCKACKTECPSSVDMAKIKTEF
                     LTHYYDKHGVPLRSRLMGHIHTLSKIAAPVAPLANLALRTPIGRPGMTMLGIHRERKM
                     SPFVQRTFVHRWRTHRKHNPVPPVTRGKAVYFHDTFATYNYPRIGMAAVHLMEAAGFE
                     VIIEERRACCGRPALSKGLVDSARKSARKNVSLLATYAKQGIPIIGTEPSCILTMRDE
                     YRDLLPGDPDVEIVAQHSFMIDEWLAKLDAEGDLGIGWKSSTGSEVLFHGHCHQKALI
                     GIDPSMSILKASGCRPTESGAGCCGMAGSFGYETEHYEVSKKIGEERLFPAVNATAMD
                     VQISVAGVSCRQQIEHFTERPTRHIAEVLPSRIDPKHTWKPKETPAAIPTAAD"
     gene            complement(4135..5058)
                     /locus_tag="VFQ54_03900"
     CDS             complement(4135..5058)
                     /locus_tag="VFQ54_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0164162.1"
                     /translation="MTTSRRGGTAVPGTEDGSVKIEILGSGGASATPRAGNDHPVDIE
                     AREKGVPYARTGPSTFIHGPNILFDTPEESRLQLVRAGISHVTACFYSHWHPDHTMGR
                     RVWESLNITGRGWPRSYRTSDVYLPEQVAEDFRTYLGGWDHLAFLESTGVIRVHEMKD
                     GDSVTIESTTVTPIKLANGYVYAFLVEEGDSSVLIAMDETEGWQPSPELRARELDLAV
                     LPIGVFEFHPLTGERRVSEDDPILEVEATFEVTLRIARSLKAREIVFSHIEALNEVGH
                     DDLLLVAERLRADGLPVTIAWDGLRINLTRP"
BASE COUNT          834 a         1560 c         1838 g          912 t
ORIGIN      
        1 cggtcgcgag gatcgcgacg aggcgacggg tcggattggc gaagatcgaa tcggtggttg
       61 gcatcggggc tcctgggttt ttgttcggaa cttggggaga cgaaccggtc cgtctcgttt
      121 caatgaagac atcatatacg agacgagacg gtccgactag tgtcacggcg gatgaatcgg
      181 tttcgccgac ctggggcgac gaccgaccgc agctgcgagc gtcgtcgcgt catcgggagc
      241 acgatcctgg ggcgaacgcg aggcgggaca ggccgtccgg actgcggtcc ggcagcggcg
      301 gtcccggccc gtcgcatacc cattcgtcat ttcgagcctg tcgagaaatc cccgtgcgac
      361 gcacctccgc cgcaggcgga catcggcggg ctctcccacg cgcgacgcgc gcctggaagc
      421 gggtcggcgc agccgcattg acccgaggcg accacgtggg tcgccgggac gggaaccgag
      481 cgcggcacag ggagggacga gacgtcgatg tctcgcccct cggcggcgca cggggcaggc
      541 acccgagcgg atgccggctg caacgcggtc gcaagtccga ggggacgtcg ctgtgaaaga
      601 cagcgacgtc gagaaccgtc gacaggcctg atggtcctgg gtttggtagg gcggggtttt
      661 ataccccgcc gcgacgcgga ttcgccaaaa ccacgccccg cgccaaggag cgccgactag
      721 tcggcggcgg tggggatcgc ggccggtgtt tccttcggct tccaggtgtg cttcgggtcg
      781 atccgggagg gaaggacctc cgcgatgtgg cgcgtcggac gctccgtgaa gtgctcgatc
      841 tgttggcggc aggagacgcc ggcgacgctg atctgcacgt ccatcgccgt cgcgttcacg
      901 gccgggaaga ggcgctcctc accgatcttc ttcgagacct cgtagtgctc ggtctcgtag
      961 ccgaacgacc ccgccatccc gcagcagccg gcgccgctct cggtcgggcg gcaaccggag
     1021 gccttcagga tcgacatcga gggatcgatg ccgatcaacg ccttctggtg gcagtggcca
     1081 tggaagagga cttccgagcc cgtgctgctc ttccagccga tcccgaggtc gccttccgca
     1141 tcgagcttcg ccagccactc gtcgatcatg aacgagtgct gcgccacgat ctcgacatca
     1201 ggatcgcccg gcaggagatc gcggtactcg tcgcgcatcg tcaggatgca gctcggttcg
     1261 gtcccgatga tcgggatgcc ctgcttggca taggtcgcga ggaggctgac gttctttcgc
     1321 gccgacttcc gggcggaatc gacgagtccc ttcgagagcg ccgggcgacc gcagcaggcg
     1381 cgtcgctcct cgatgatcac ctcgaaaccg gccgcttcca tcaggtggac ggccgccatc
     1441 ccgatccgcg ggtagttgta ggtggcgaag gtgtcatgga agtagaccgc cttgccccgc
     1501 gtgaccggcg gcaccgggtt gtgcttgcga tgcgtgcgcc agcggtggac gaaggtccgc
     1561 tgcacgaacg gcgacatctt tcgctcgcgg tggatgccga gcatcgtcat ccccggtcga
     1621 ccgatcggcg tccgcagcgc gaggttcgcg agcggggcga ccggcgccgc gatcttcgag
     1681 agcgtgtgga tgtgtcccat cagccgggag cgcaatggaa caccgtgctt gtcgtagtag
     1741 tgggtgagga actcggtctt gatcttcgcc atgtcgacgc tggacgggca ttccgtcttg
     1801 caggccttgc aggagaggca gagatcgagc gtctcgtagg tcgccttgtc gccaaaatcc
     1861 ttgggcgaga ggatattccc ggtgagcgca ttgcggagcg cgttcgcgcg gccgcgggtg
     1921 gtgtcgttct cgtccttcgt cgccatgtag gaggggcaca tcgtgccgga cttcgtctgg
     1981 cgacagaccg ccgcgccgtt gcacatctcg acggcggcca tgaagccgcc ctcgcgggag
     2041 aagtcgagat tggtgcggat cggcaccggc ttgtacttcg gaccgtagcg gaggttgtcg
     2101 acgagcggcg gcgcatccac gatcttgccc gggttcatca ggtcgttcgg atcgaagaga
     2161 tgcttgaagt cgcgcatcgt ctggtagagc tcgggaccga agatgatcgg attcagctcg
     2221 gagcgctgga gcccgtcgcc gtgctcgccg ctcatcacgc cgccgaacga gtgggcgatc
     2281 tccgccgcct cgtaggcgag ctcgcgcatc gcctcgacac cttcgaccgt cttcaggttg
     2341 atcagtgggc ggatgtgaag gcagccggcg gaggcgtgcg cgtagaacgc cgccgtcgtg
     2401 tggaagcgcg cgaccatctg ctcgacggcg gcgacaaacg cggcgagatg ctcgaccggg
     2461 accgagacgt cctcgatcgc cgggatcggc ttcgcatcgc cacggacgct catcaggagt
     2521 ccgagcccgg ccttgcggac ctgccagaca tccttctgct gcttcggatc gaggacgacc
     2581 tgcggatcgg tcatcagcgt gatgttgcgc tgccggaggt ggtcgaccag gcgatcggcc
     2641 ttctgctgca ggtcgcgctc gttctctcca tagaactcga cgacgagcac ggcggccgga
     2701 tcgccctcga tcatcgagat ctgcttcgcg tagcccggct gctggcgggt gagaccgatc
     2761 agcatccgat ccatcaactc gatcgcgctc ggctcgacct cgaggatctg cggcgtgact
     2821 tccatcgcgc gaacgaggtt gtcgaacgag agcatgacga gacccgtgat cggggggacg
     2881 ggaacgaggt tgagcgtgat ctgcaggagg ctgccgagcg tgccttccga cgaggcgagg
     2941 aggcgcgccg ggttgaagac atcgtccggc ttcaggaact cgttgagcga gtagccggtg
     3001 gcgcgccgcc agtgcggcgg gaagtcgcgg gcgatgagtt cctggcgctc ctcgcggaag
     3061 gcgaggagct tcttcaggag gcggcccgtc gcgtcgtcgg tctgggagcg cttgagaaga
     3121 tcctccatct tgcccggccc gagttcgacc gtgctgccat ccgcgagctg catgcgcgcg
     3181 gactggagat tgtcctgcgt cattccatag aggatcgagt gcgccccggc ggcgttgttg
     3241 ctgacgacac caccgacggt ggcgcgattc gacgaggatg gatccggacc gaacatcagg
     3301 ccgtggctct tcaactggcg gttgagggcg tcgaggttga tcccgggttc gaccgtgacg
     3361 gtcctgttct cgggattgac gctgatcagg cggtcgaggt atttcgaggt atcgagcacg
     3421 agcgctgcgc cgacggcctg gccggcgagc gaggaaccgc cgccgcgagt gaggatcggt
     3481 acgctgtggc tggtcgcgag ttcgatcgcg gcgatcacgt cgtccgtgga gcgggggatg
     3541 acgacgccga ccggttcgat ctcgtagttc gaggcgtcgg tcgcatagag catgcggctg
     3601 acggcatcga agcggacctc accctggacg cgggccgcga gttcggcctc gaggacgcgg
     3661 gcggtctcga tcgcgccacg gtaggagcga tccggtttcg tggagacgga cgaggagcgt
     3721 ggcggcgttg gcagggttgg gattgggggt ggcgtcgcac cattggtggt cgccggttgg
     3781 cggttgagcg ccatgagccg cgtctccttt gtactggaac tggcgatgcg tgtggcgccg
     3841 gagaacgatc ggcgaccggc agccgtcacg atagtgacga cgagtttagc accgggtcga
     3901 atcgcgggac agtttgcggg cgatggaatc gcctcatcgg gcggggcaat gggagcacgc
     3961 tcttcgggcg aaggcgaggc gggacaggcc gtccggacct gcggtccggc agcggcggtc
     4021 ccgccccgcg tacgaccggg gtatcgccga cccccgggtc tgtagggcaa gaccttgtgt
     4081 cttgccgcat gccgcgaacg ggatcgctcg aacaagatcc ggaacggaac cccgctaggg
     4141 gcgcgtcaag ttgatccgga gcccgtccca ggcgatcgtt accgggagac cgtccgcacg
     4201 gagacgctcg gcgacgagga gcaggtcgtc gtggccaacc tcgttgaggg cctcgatgtg
     4261 actgaagacg atctcccggg ccttcaggct gcgcgcgatc cggagcgtca cctcgaaggt
     4321 cgcttccacc tcgaggatcg ggtcgtcctc gctgacgcgt cgctcgccgg tgaggggatg
     4381 gaactcgaag acgccgatcg gcaggaccgc gagatcgagt tcacgcgccc ggagttccgg
     4441 cgatggttgc catccttccg tctcgtccat ggcgatgagc acagagctgt cgccctcttc
     4501 gacgaggaag gcatagacgt agccattcgc gagcttgatc ggtgtgacgg tggtgctttc
     4561 gatcgtgacc gaatcgccgt ccttcatctc gtggacgcgg atcacgccgg tgctttcgag
     4621 gaaggcgaga tgatcccagc cgccgagata ggtgcggaag tcctcggcga cctgttccgg
     4681 gagatagacg tcgctcgtcc gatacgacct tggccagccg cgaccggtga tattgagtga
     4741 ttcccagacg cggcgaccca tcgtgtgatc ggggtgccag tgggaataga agcaggcggt
     4801 gacgtgcgag atgccggcgc ggacgagttg gagccggctc tcctcgggcg tatcgaagag
     4861 gatgttcggt ccgtgaatga acgtgctcgg tccggttcgg gcgtacggaa cgcccttctc
     4921 ccgcgcctcg atatcgactg ggtggtcgtt cccggcgcgt ggtgtggcgg acgcgccacc
     4981 gctgccaaga atctcgatct tcacgctgcc atcctccgtg cccggcacgg cagttccgcc
     5041 gcgccgtgaa gtcgtcacta ccctccggga gggtagtgcc aagcatacgt ggaacgacgc
     5101 gaatgggtat cctgaggctg cgactcgcac gtctgcggta gcag
//
LOCUS       DASTMD010000261         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1542204, whole genome shotgun sequence.
ACCESSION   DASTMD010000261 DASTMD010000000
VERSION     DASTMD010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542204"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFQ54_03905"
     CDS             complement(<1..383)
                     /locus_tag="VFQ54_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164163.1"
                     /translation="MKRIGEVMRDFPVPPRPAGASPNGAIRITPRETVNCAICNDAGY
                     VRMNVPIGHPMFGKLVMCVCREKELSDRRMEDLHRLSNLDAFTHHTFEEFDPSLHGLE
                     HAYEEALAFAQEPGQRWLFLYGPCGV"
     gene            complement(392..1006)
                     /locus_tag="VFQ54_03910"
     CDS             complement(392..1006)
                     /locus_tag="VFQ54_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEU0164164.1"
                     /translation="MPIPQAFFDDVLPGITSLEELHVSLAIFRLTHLAGGYDEPIAER
                     SLIRDRNLRNAMKVDGSPREPDRRIEHGLELAVARGTLLRLEVRSGRRPSIWYYVNTP
                     FNRSSVAAMERGAVPAPATVWETDAQPEVRVERPNAFRLYEQNIGPLTPLIADQITRA
                     IDEYPGDWIEDAIVESVSYNRRSWRYITRILESWATNGRGDSDR"
     gene            complement(1069..2409)
                     /gene="dnaB"
                     /locus_tag="VFQ54_03915"
     CDS             complement(1069..2409)
                     /gene="dnaB"
                     /locus_tag="VFQ54_03915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU0164165.1"
                     /translation="MDTAVERLPPHNIDAEQSVLGSLMIDRDAIIRVASYLKPEDFYI
                     WANGQIYQAVLDLYNVRRPTDLVTLSDELKNRGVLDDIGGDVYLSSLLGSVPTAVHVE
                     YYGRIVERMATRRRLIDAGTEIVGIGYRDSMETEDALDSAERAIFDVSQKRQTKDFQS
                     ISDVLDRFFDQIEYLQQHRGDLVGVPTGYTDLDALLGGLQKSDLLIFAARPAMGKTAF
                     LLGLAYGAAVTHGRTVGIFSLEMSADQLVQRVLATETGIDSHRLRLGMIDDNEWDRVS
                     RAFGRLAEANIFIDDGAAATIMDIRSKARRLQAEQGLDFLIIDYLQLMSGRRTENRVQ
                     EVSEISRGLKGLARELNIPVIAAAQLSRAVETRADHRPLLSDLRESGSIEQDADIVMF
                     LHREEKYDENSEKKGIAEIIVAKHRNGPVGSVNLRFFERTGRFADLELYRDPEN"
BASE COUNT          428 a          713 c          852 g          452 t
ORIGIN      
        1 acgccacacg gcccatagag gaagagccag cgctgacccg gctcctgggc gaaggcgagt
       61 gcttcctcgt aggcgtgctc gagaccgtgc aggctcgggt cgaactcctc gaaggtgtga
      121 tgcgtgaagg catcgagatt cgaaaggcga tggagatcct ccatccgcct gtcgctgagt
      181 tccttctccc ggcagacgca catcacgagc ttgccgaaca tcggatgtcc gatcggcacg
      241 ttcatccgga cgtagccggc gtcattgcag atggcgcagt tcacggtctc gcgcggcgtg
      301 atacgaatgg ctccgttcgg cgaggcgccg gcgggccggg ggggaacggg aaagtcgcgc
      361 ataacttcgc cgatgcgttt catggactct gctacctgtc cgaatcgccg cgaccattgg
      421 tcgcccacga ttcgagaatt cgtgtgatgt agcgccagct gcgccggttg taggagacgg
      481 attcaacgat cgcgtcttcg atccagtccc ccgggtattc gtcgatcgcc cgcgtgatct
      541 ggtcggcgat caacggcgtg agtggcccga tgttctgctc gtagagccgg aaggcattcg
      601 ggcgctcgac gcggacttcc ggttgcgcat ccgtctccca gaccgtcgcc ggagcgggaa
      661 cggcgccccg ctccatcgcc gcgacgctcg accggttgaa cggcgtgttg acatagtacc
      721 aaatgcttgg ccgccggcct gaccggactt ccaggcggag cagcgttccg cgggcgacgg
      781 cgagttcgag cccgtgttcg atccggcgat ctggctcgcg ggggctgcca tcgaccttca
      841 tcgcgttgcg caggttgcga tcgcgaatca gcgagcgctc ggcgatcggt tcgtcgtagc
      901 cgccggcgag gtgggtgaga cgaaagatgg cgagcgagac atgaagttcc tcgaggctcg
      961 tgattcccgg cagcacgtca tcgaagaatg cctgcggaat aggcacggcg ggatccgtgg
     1021 ccacgaggaa gcccccgaac gcggggcccg cgagagacgt ccggggcatc agttttccgg
     1081 atcgcggtag agctcgagat cggcgaaacg accggtgcgt tcgaagaagc gcaggttgac
     1141 gctccccacc gggccgttgc ggtgcttggc gacgatgatc tcggcaatgc ccttcttctc
     1201 gctgttctcg tcgtactttt cctcacggtg gagaaacatc acgatgtcgg catcctgctc
     1261 gatcgatccc gactcacgaa ggtcggagag gagcggccgg tggtcggcac gcgtctcgac
     1321 ggcgcgggag agctgggccg cggcaatgac cgggatgttg agctcgcgcg cgaggccctt
     1381 gaggccacgg gaaatctcgg aaacttcctg gacgcggttc tcggtccggc ggccgctcat
     1441 cagctggagg tagtcgatga tcaggaagtc gagtccctgt tccgcctgga ggcgtctcgc
     1501 cttcgaccgg atatccatga tcgtcgcggc ggcgccgtca tcgatgaaga tgttcgcctc
     1561 ggcgagacga ccgaacgccc gcgagacgcg atcccattcg ttgtcatcga tcatcccgag
     1621 ccggagccgg tgcgagtcga tgcctgtctc ggtggcgagc acgcgctgga cgagctggtc
     1681 ggccgacatc tcgaggctga agataccgac cgtgcgaccg tgggtgacgg cggcaccata
     1741 ggcaagaccg agcaggaagg cggtcttgcc cattgccggt cgggcggcaa agatcaggag
     1801 gtcggatttc tggaggccgc ccagcagggc gtcgagatcc gtgtagccgg ttggcacgcc
     1861 gacgagatcg ccgcgatgtt gctggagata ctcgatctga tcgaagaagc ggtcgaggac
     1921 gtcggagatc gactggaagt ccttggtctg gcgcttctgc gagacgtcga agatggcccg
     1981 ttcggcgctg tcgagcgcgt cttcggtctc catgctgtcg cggtagccga tgccgacgat
     2041 ctcggtgccg gcgtcgatca ggcggcgacg tgtcgccatg cgctcgacga tgcggccgta
     2101 gtattcgaca tggaccgcgg tcggaacgct gccgaggagg gacgagaggt agacatcgcc
     2161 gccgatgtcg tcgaggacac cgcgattctt caactcgtcg gagagggtga cgaggtcggt
     2221 tggacggcga acgttgtaga gatcgaggac ggcctggtag atctgcccgt tggcccagat
     2281 gtagaagtcc tcaggcttga ggtacgacgc aacacgaatg atcgcgtcgc ggtcgatcat
     2341 gaggcttccg aggacagatt gctcggcgtc gatattgtga ggtggtaggc gctcgactgc
     2401 tgtatccatt cagagggctc cctctgaggg atgagccggc ggggg
//
LOCUS       DASTMD010000262         1694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_471065, whole genome shotgun sequence.
ACCESSION   DASTMD010000262 DASTMD010000000
VERSION     DASTMD010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471065"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /gene="uppS"
                     /locus_tag="VFQ54_03920"
     CDS             <1..652
                     /gene="uppS"
                     /locus_tag="VFQ54_03920"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU0164166.1"
                     /translation="ARQGLARIEGHERGTENIRRITQRAGELGIEYLTLWAFSTENWR
                     RPKEEVDGILAILGRAIESETAELHRQGAQLRHIGELDALAPDLQDSIRTAIALTSGN
                     TKLVLTLAFNYGGRHEVLQAVRQVIRDGLTPEEISEETFAERLFTKDTPDPDLIIRTS
                     GEYRMSNFLIWQGAYSEFFFSPKFWPDFGPDDLSEAVEDYARRERRFGAVSAPVTR"
     gene            658..1515
                     /locus_tag="VFQ54_03925"
     CDS             658..1515
                     /locus_tag="VFQ54_03925"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU0164167.1"
                     /translation="MRQRAISSIVVVLIGLVPAIVGGPLFAIVFALIAMLAYHELIAI
                     LGLEPDILVMSGYALVAFAAIAAYSSPTRLFPVYVALSIFLPVIIGVLDPQVEEDILL
                     RWGRVSVASLYVGIAAFAAIALRQLEGVPKTGWFDSIGSGLTFTDRDTSIGLAWLLLA
                     ILVTWLSDTVAYLVGRSVGRTPLIPRVSPKKTVEGAVGGFIAAGLTALICILVFGIDI
                     NPLTGLLVGLLLGGIGMLGDLCESLLKRKAGIKDSGTLIPGHGGVLDRIDALIFVIVA
                     TWLIVPFLT"
     gene            1571..>1694
                     /locus_tag="VFQ54_03930"
     CDS             1571..>1694
                     /locus_tag="VFQ54_03930"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU0164168.1"
                     /translation="MLGSTGSIGRQTLDVVAHQPDRFRIATIAAGKQRALLEEQV"
BASE COUNT          273 a          572 c          510 g          339 t
ORIGIN      
        1 tgcccggcag ggcctggcgc gcatcgaggg tcacgagcgc ggcacggaga atatccgccg
       61 catcacccag cgtgccgggg aactcgggat cgagtacctc acgctctggg cattctcgac
      121 ggagaactgg cggcgcccca aggaagaggt cgacggaatt ctcgccatcc tcggtcgcgc
      181 gatcgaatcc gagaccgccg aacttcatcg ccagggcgcg caacttcgcc acatcggcga
      241 gctcgacgcg ctggcgccgg acctccagga ctcgattcgc accgcgatcg ccctcacgag
      301 tggaaacacg aagctggtgc tgaccctcgc cttcaactac ggcggtcgtc acgaggtcct
      361 gcaggccgtc cggcaggtga tccgcgacgg attgactccc gaggagatct ccgaggagac
      421 cttcgccgag cgcctcttca ccaaggacac gccggatccc gacctgatca ttcgcacatc
      481 cggtgagtac cggatgtcga acttcctgat ctggcagggt gcatactccg agttcttctt
      541 ctctccgaag ttctggccgg atttcggtcc cgatgacctg agcgaggcgg tcgaggatta
      601 cgctcgccgc gaacggcgct tcggagcggt ttccgctccc gtcacccggt agcggtcgtg
      661 cgccaacgag cgatctcatc gatcgtggtc gtcctgatcg ggctggttcc ggcaatcgtg
      721 ggcggtccgc tcttcgcgat cgtgtttgca ctgatcgcga tgctcgccta ccacgaactc
      781 atcgccatcc tggggctgga gccggatatc ctcgtgatgt ccggctacgc gctcgtcgcg
      841 ttcgcggcga tcgccgcgta tagctccccc acccgcctct ttccggtgta cgttgccctc
      901 tccatcttcc tgcccgtgat catcggggtg ctcgatccgc aggtcgagga agatatcctc
      961 ctccgctggg gtcgggtcag tgtcgcgagc ctctacgtcg gcatcgccgc cttcgccgcg
     1021 atcgcgctcc gtcagctcga aggcgtcccc aagaccggct ggttcgattc gatcggctcg
     1081 ggactcacct ttaccgaccg cgatacatcg atcgggctcg cgtggcttct gctcgccatt
     1141 ctcgtcacct ggctgtccga caccgtcgcc tatctcgtcg ggcgaagcgt cggccgaacg
     1201 ccactgattc cccgcgtcag cccgaagaag accgtcgagg gcgcggtcgg cggattcatc
     1261 gcggccggcc tcacggcgct gatctgcata ctcgtcttcg gaatcgacat caatccgctg
     1321 actgggctgc ttgtcggtct tctgctcggc ggcatcggca tgctgggcga tctctgtgaa
     1381 tcactgctca agcgcaaggc ggggatcaag gacagtggca cactgatccc gggccatggc
     1441 ggcgtacttg accgtatcga tgcccttatc ttcgtgatcg tcgcaacctg gttgatcgtt
     1501 ccgttcctta cctgattcaa tcgcgatcga ccgcgctcag gagactcccg tgccacaaac
     1561 gacgttggct gtgcttggct cgacaggatc gatcggtcgt cagaccctgg acgtcgtggc
     1621 acaccagccc gatcgcttcc gcatcgcgac gatcgcggcc ggaaagcagc gagcactgct
     1681 cgaggagcag gttc
//
LOCUS       DASTMD010000263         4888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1988057, whole genome shotgun sequence.
ACCESSION   DASTMD010000263 DASTMD010000000
VERSION     DASTMD010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988057"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VFQ54_03935"
     CDS             <1..1179
                     /locus_tag="VFQ54_03935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU0164169.1"
                     /translation="DGVREPALRDGLRACWSTPIRDHGTDRVFGTFAIYHRSPRVPTT
                     VEITMVEQTGRLAGMAIKHNEVRQAQQRSEARFRALVQHAADITALLAPNGTIKYISP
                     AVIDILGGDPEKIVGLRFLDYLHPEDRRATAAFLSDLRHGREMPSMRIEFRIPDNDGE
                     WHWIEAIVTDLSHDENVGGIVVNARDITQRKDAEQQLHYLAATDALTRLPNRSHFAQR
                     LTAAIDERGEGESGPTIIFLDLDYFKAVNDDYGHAVGDQLLVAVGERLQGALQRGDIL
                     ARWGGDEFTILLQDRQEALPTSTRILAAFSRPFLIGPRTLAVSPSIGIATGAPGSDTA
                     RLVHEADLALYRAKTTRRACIAVFDPGRDYGATSLSGVSGGTFGSRIQIERSETSPSA
                     "
     gene            complement(1199..1909)
                     /locus_tag="VFQ54_03940"
     CDS             complement(1199..1909)
                     /locus_tag="VFQ54_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0164170.1"
                     /translation="MTATPNPWNDYATEYWNEVQRRGPLVADDNGMAARMLDLLGDLT
                     GKTVLDAGCGEGLLSRLFASRGATVTGVDLSPRLLEMARMQDPTGAIDYRVGDLGEPH
                     PELAGRFEAIGSYLVLNDVADHRGFARSLSASAKPDAPVVLAFNNPYSSIVREHIIDY
                     FDMEERGTYLGMGERGIHAQYYHRTLEEYLDAFISTGLRLERLVDVKPGITHRVLPEG
                     ATFPNFMILAFRKPGQPA"
     gene            2112..2525
                     /locus_tag="VFQ54_03945"
     CDS             2112..2525
                     /locus_tag="VFQ54_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0164171.1"
                     /translation="MPVLVWLRVARIYQKIDHRTAETMKVWNISVSRFDVINQIGAHE
                     GITQQELADVLLVTKGNITQLLDALEKEGTVERKRYGRTKRLYLTDCGRTLREGSMRD
                     QETLLDKEFAVLDDKEMDTLLSLLRKIDRSLSETG"
     gene            2590..3654
                     /locus_tag="VFQ54_03950"
     CDS             2590..3654
                     /locus_tag="VFQ54_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEU0164172.1"
                     /translation="MALANGLELGGLHHVSSVTANIEENRDFYTRALGMRMVKKTVNQ
                     DATDMYHLYYADALGTPGANVTFFDYEGSARTSYTRIAPNQAGSGEINEIALRVSGPE
                     ALTYWRERFDKLGIPYGPMTTRGGHAAFAFTDPEGQRLALIDDSGATIDGGTPWELAT
                     VPQEFGIKGLGAISTSTARPEATLQVLTDFLGFRIVSDTPDAVVPGNRTVVLETGKGG
                     PGGLFIVHVTPNEPRAHLGAGGVHHIAFRVDTFENHERWNEYLSAAGFHVTPVIDRFY
                     FKAMYFREPGGVLYEISSDAPGFATDEPLESLGESLALPPFLEDERPTIERAIRPLDT
                     RKIVTTTEYGQPVPEVSVSK"
     gene            3651..4316
                     /locus_tag="VFQ54_03955"
     CDS             3651..4316
                     /locus_tag="VFQ54_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0164173.1"
                     /translation="MSQSTTSLPHANAPVFHTGAPLAEAELVIILNHGRGSTADNILG
                     LAPAITPEEDAGAKIAFLAPQADGNVWYPQPFRQPLASNEPWLTGALATIDRIVDLVR
                     AETPDRKIVVGGFSQGACLSLEYLARGSREISGVAAFSGALIGPSISDRQAIPTLDGR
                     WVFLGCGDRDSYFTIDVAEDSVAALKAAGAETEFRQYPGMGHTIVEDETNAVRAKIAS
                     LLP"
BASE COUNT          911 a         1640 c         1484 g          853 t
ORIGIN      
        1 gacggggttc gcgaaccggc gcttcgtgat ggactgcgtg cctgctggtc gacgccgatc
       61 cgcgaccatg gcacggaccg cgtgttcggc accttcgcga tctatcatcg ctcgccccgc
      121 gtcccgacca cggtcgaaat cacgatggtg gaacagaccg gccggctcgc cgggatggcg
      181 atcaagcaca acgaggtgcg ccaggctcag cagcgaagcg aggcgcgctt tcgcgccctc
      241 gtccagcatg ccgccgacat cacggcgctg ctcgcgccga acggcacgat caagtacatc
      301 agccccgcgg tgatcgacat cctcggtgga gatccggaga aaatcgtcgg cctgcgcttc
      361 cttgactacc ttcacccgga ggatcgccgc gcgacggccg cgttcctttc cgatctccgc
      421 cacggccggg agatgccgtc gatgcgcatc gagttccgca ttccggacaa tgacggcgag
      481 tggcactgga tcgaggcaat cgtcaccgac ctctcgcatg acgagaatgt cggcggaatc
      541 gtcgtcaacg cgagggacat cacgcagcgc aaggatgccg agcaacagct ccattacctg
      601 gccgcgaccg atgccctgac ccggctcccc aaccgttcgc acttcgccca gcgcctcacg
      661 gccgcgatcg atgaacgggg cgaaggggaa tcgggaccca cgatcatctt cctcgatctc
      721 gattacttca aggccgtcaa cgacgactac gggcacgcgg tcggcgatca actcctggtt
      781 gccgtcggcg aacgcctgca gggcgccctt cagcgaggcg acatcctcgc ccggtggggc
      841 ggcgacgaat tcacgatcct gcttcaggac cggcaagaag cccttcccac gtcgacccgc
      901 atcctcgccg ccttctcgcg accgttcctg atcgggccac gcacgctcgc tgtctcgcca
      961 agcatcggga tcgccaccgg cgccccgggc tccgacaccg ccaggctcgt gcatgaggcc
     1021 gacctcgcgc tctaccgggc caagacgacg agacgcgcct gcatcgcggt cttcgatcca
     1081 ggccgcgatt atggtgcgac ctcgttgtcc ggtgtctccg gtggaacctt cggctcccga
     1141 atccagatcg agcggagtga aacctcgcca tccgcatagc ggcagcgacg gcctcgtatc
     1201 aggcaggctg accgggtttc cggaacgcca ggatcatgaa gttcgggaag gtagcgccct
     1261 cgggcaacac gcgatgcgtg atgccaggct tcacgtcgac gaggcgctcg aggcgaaggc
     1321 ccgtgctgat gaaggcgtcc aggtattcct cgagcgtgcg atggtagtac tgcgcgtgga
     1381 ttccgcgctc ccccatcccg agataggttc cacgctcctc catatcgaag taatcgatga
     1441 tgtgttcccg tacgatcgac gaatacgggt tgttgaacgc aagaacgacc ggggcatcgg
     1501 gcttcgcgct cgcggagagc gaccgcgcga agccgcgatg atccgcgacg tcgttgagga
     1561 cgagatagct tccgatcgcc tcgaacctcc cggcgagttc cgggtgtggt tcgccgagat
     1621 ccccgacccg gtagtcgatc gcaccggtgg ggtcctgcat ccgcgccatc tccagcagcc
     1681 gcggcgagag atcgactccg gtcaccgtcg cgccacgtga ggcgaacagg cgggagagca
     1741 gcccttcacc acagccggcg tcgagcaccg tcttcccggt caggtcgccg agcagatcca
     1801 gcatgcgcgc ggccatgccg ttgtcatcgg caacgagggg accgcgccgc tggacctcgt
     1861 tccagtattc ggtcgcatag tcgttccagg gatttggagt cgcggtcatc ctgatctcct
     1921 ctcacgcgtc gtgacgttgc ggttgcacga tcgatgatag cgaccgggct gattgacggg
     1981 gccggacgac acgtgcatcc ggggttgaca gcctcacagt ttagttctaa actattcata
     2041 tggatacgac gacagagtcc ttggaacaac cagatctccc tctctcacag cgtcgtcggg
     2101 cggagattcc cttgcctgtt ctcgtgtggc ttcgcgtcgc ccgcatctat cagaagatcg
     2161 accaccgtac ggcagagacc atgaaagtgt ggaatatctc cgtcagtcgc ttcgatgtga
     2221 tcaaccagat cggcgcgcac gagggcatca cccagcagga actcgcggat gtgctgctcg
     2281 taacgaaagg aaacatcacc cagttgctcg atgcgctcga gaaggagggt acggtcgagc
     2341 ggaagcgcta cgggcgcacg aagcggctct acctgaccga ttgcggccga acgcttcgcg
     2401 aagggtcgat gcgcgaccag gaaacgctgc tcgacaagga atttgccgtt ctcgatgaca
     2461 aggagatgga tacgctgctt tccctcctcc gcaagatcga tcgttccctc tccgagaccg
     2521 gatgacgcag gcgattggca tttccccagt tttcggacct gttctgaatc tcccgaaagg
     2581 acttcccgga tggcactcgc aaacggcctc gaactcggcg gcctgcacca cgtcagttca
     2641 gtgaccgcga acatcgaaga gaatcgcgac ttctacaccc gcgccctcgg catgaggatg
     2701 gtcaagaaga cggtcaacca ggacgcgacc gacatgtacc atctctacta tgcggacgcg
     2761 ctcggcacgc cgggcgccaa tgtcaccttc ttcgactacg agggttccgc tcgcacctcc
     2821 tatacccgga tcgcgccgaa ccaggccggc agcggcgaga tcaacgagat cgcgctgcgc
     2881 gtttccggcc ccgaggcact cacctactgg cgggaacgat tcgacaagct cggcatcccg
     2941 tatggtccga tgacgacccg cggcggtcac gccgcctttg ccttcaccga tccggagggg
     3001 cagcggctcg cgctgatcga cgactccggc gccacgatcg acggcgggac gccgtgggaa
     3061 ctggcgacgg ttccacagga attcggaatc aaggggctcg gcgcgatctc cacatcgacc
     3121 gcccgtccgg aagcgacgct gcaggtcctc acggacttcc tcggtttccg gatcgtttcc
     3181 gacacaccgg acgccgtcgt tcccggtaac cgcaccgtcg tgctggagac cggcaagggt
     3241 ggaccgggcg gtctcttcat cgtccatgtc acgccgaacg agccacgcgc ccacctcggc
     3301 gcaggtggag tccatcacat cgccttccgc gtcgacacgt tcgagaatca cgagcgatgg
     3361 aacgaatacc tctccgccgc cggcttccac gtgacgccgg tgatcgaccg cttctacttc
     3421 aaggcgatgt acttccgcga accgggtggg gtgctctacg agatctcctc ggacgcgccg
     3481 ggcttcgcga ccgatgagcc gctcgaatca ctcggcgaat cgctcgcgct gccgccattc
     3541 ctcgaagacg agcggccgac gatcgagcgc gcaatccgac cgctcgacac ccgcaagatc
     3601 gtcacgacga cggaatacgg tcagccagtc ccggaagtga gcgtcagcaa atgagccagt
     3661 ccaccacgtc gcttccccac gccaacgcgc cggttttcca caccggcgcc cccttggcgg
     3721 aggccgaact cgtcatcatc ctgaatcacg gtcgcggttc gaccgccgac aacatcctcg
     3781 gtctcgcacc cgccattacg ccggaagaag atgccggcgc gaagatcgcg tttctggcac
     3841 cccaggcgga cggtaacgtc tggtacccgc agccatttcg ccagccgctg gcgtcgaacg
     3901 agccgtggtt gacgggcgca ctcgcgacga tcgatcgcat cgtcgatctc gttcgcgccg
     3961 agacaccgga ccggaagatc gtggtcggtg gattctcgca gggagcatgc ctctcgctcg
     4021 aatacctggc gcggggatcc cgcgagatct cgggagtcgc ggcattcagc ggcgcgctga
     4081 tcggcccctc gatctcggac cggcaggcga tcccgaccct ggacggtcgc tgggtcttcc
     4141 tcggctgcgg tgaccgcgac agctacttca cgatcgatgt cgccgaggat tcggttgccg
     4201 ccctcaaggc agctggagcc gaaacggagt tccgccagta ccccggcatg gggcacacga
     4261 tcgtcgagga cgagacgaac gcggtgcgcg cgaagatcgc ctccctcctc ccgtaacatc
     4321 cggaacccgc gccggttgaa taggactacg cttgcccatt ccagcaagca gcgggtcacc
     4381 ggtcagagcg gtgagcgcgc aggatgcagg gggaggtggg acagtcgttc accaccgacc
     4441 gcgctctccc tgagcgtggt gagcgcggtc ggtggtgaac gactgtcccg ccctacgaac
     4501 gatcgacatc cgtgcagcgt tcgtgagtac ggcgggactt ctatgtcccg cccaccgtgt
     4561 gatcgccgaa ccgaagagcc tgccctgagc acagccgaat gggttcggga ctcccatccc
     4621 gccttttgct ctcacgggca gggcctttca gccagcgggc tcgtcgcaaa tgcgacgagc
     4681 ccgttttgct gtcattctct ccttcagaag cgagtcggcc cgcccgaaat cgccatcatg
     4741 caacgtccat ttgcactgtg gcattgcgtg gtgggatatc acatgctatc gttggcgcat
     4801 tcgaacaacc agagggacgt tatgaccgag tcttcgccgg aactggccct gccagccggt
     4861 atctcgcaac gccagattgc ggaccgta
//
LOCUS       DASTMD010000264         4395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_112697, whole genome shotgun sequence.
ACCESSION   DASTMD010000264 DASTMD010000000
VERSION     DASTMD010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112697"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VFQ54_03960"
     CDS             <1..738
                     /locus_tag="VFQ54_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0164174.1"
                     /translation="VIVFQDMTRLREAEQLKDDFLSLVSHEFRTPLTAIHGGARLLGS
                     QRDQLDDRTKDELLADIVLESARLDQMLANMLQLAEVMAGRLTPSTEPVLVTPLIRRV
                     VQEMKVRLPDTEWSIEIPPGLPPVEGDPSLLQQVLRNLYENAIKYTTGHAFIRTTAEV
                     DEHGVEIHIIDNGIGIAAEHVPFVFERFRRPGADPAVRGMGLGLYLCHNLMTAQGGRI
                     RVESHGIGMGTTFSIALPIASGWDADE"
     gene            767..1501
                     /locus_tag="VFQ54_03965"
     CDS             767..1501
                     /locus_tag="VFQ54_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0164175.1"
                     /translation="MRKRLILIVDDEPPIARLSRAKLEADGYAVVLATNGEEALRRIE
                     EDRPDLVVLDVMMPVMDGFEVLRRLRATSQLPVILLTARTSDIDKLTGLHGGADDYLT
                     KPFNPDELSARIAAIMRRSAGVGIVTNHQLMTYPATGVLPEVTIDIEQRRIMVGGAEV
                     RLSRTEWELLTQLAANSGRVMLHGELLSRIWGPEFRDEVQYLRTWISRIRTKLEPSAS
                     DGSRIITTFPGLGYRMESPVAMPPAE"
     gene            complement(1509..2363)
                     /locus_tag="VFQ54_03970"
     CDS             complement(1509..2363)
                     /locus_tag="VFQ54_03970"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0164176.1"
                     /translation="MTQRSTNALLGYADDARLLIVNADDFGMCHAVNEAVLQGFTRGI
                     ITSTSLMVPCAWAPDAMRILDDHPELPFSVHLTLVSEFGGYRWGPVASKDRIASLIDA
                     SGYFPVYDQIPDLLARADIGEVEIEFRAQIEAALAAGLTPSHLDWHCIADGGREDIFD
                     LCYRLAREYGVAMRIHRSGHAERLRREGLPVVDHDVLDSYHMGFEDKANRFAALLRAL
                     PPGLTEWAVHPSLGDDESRAIEPETWQIRRADHDFVTSPEARDLIDELGIVLLDYRAL
                     QTVWNVGN"
     gene            complement(2423..3058)
                     /locus_tag="VFQ54_03975"
     CDS             complement(2423..3058)
                     /locus_tag="VFQ54_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0164177.1"
                     /translation="MTTPSAAPGLRERKKIMTRSTILSVAEALFEERGYDNVTVAEIA
                     DGANVSVKTLFTYFRSKEDLAFGEADWLIDAIVDDLRARDPGISPVDAVAGTLKRLVA
                     DPDRPGLGVEGFHRGVGDSLVLESRLLRMWADYEDTLTAELAREAGEASPSPRVRLMA
                     IQLIAILRTVTSQEIRRMLGDLPMESQQGALTAWIDEAASLTAGHVDARLS"
     gene            complement(3224..4096)
                     /locus_tag="VFQ54_03980"
     CDS             complement(3224..4096)
                     /locus_tag="VFQ54_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164178.1"
                     /translation="MIWTLTGRHLRIFFRDRMGVFFALLSPLILFALYKLFLGNIQVN
                     NLKDDWPAANHDDAQHFVDAWVFAGTLMLTTLTAGLGAMTVFVDDRASGRFADFVVSP
                     IRRRDLILGYSLTSFLIALSISLFVLVASQLITVVTGASLMHPADWLRCVGSVIVMCA
                     AFSAISGLAVTFTATSGAFSSLSAIVGTFAGFLAMAYIPAQALPHGVVRVLNWLPFAE
                     AAMLVRGPLTSRSLAALTHGQPQAAATVRANYGISISLAGHHAAASLIAAVLAAIAII
                     FAAIGSWRIRRVVA"
     gene            complement(4093..>4395)
                     /locus_tag="VFQ54_03985"
     CDS             complement(4093..>4395)
                     /locus_tag="VFQ54_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164179.1"
                     /translation="DHGTIVAEGTPTALRAAHARDELQLVARSADAIRQDLEARGYVF
                     SQQGDVLRIGVGSSQDALTLLKRHEAELVDFEFRHGTMDDVFLSLVDPTSGRRRVE"
BASE COUNT          867 a         1413 c         1394 g          721 t
ORIGIN      
        1 gtgatcgtct tccaggacat gacgcgtctc cgcgaggccg agcagctgaa ggacgacttc
       61 ctctcgctcg tctcgcacga gttccggaca ccgctcaccg ccattcacgg gggagcacgg
      121 ttactcggat cccagcgcga ccaactcgac gatcggacga aggacgagct cctcgcggat
      181 atcgtgctcg agagcgcccg gctggaccag atgcttgcca atatgctcca gcttgccgag
      241 gtgatggccg ggcgcctgac gccgtcgacc gaacccgtac tcgtgacgcc gctcatccgg
      301 cgcgttgtcc aggagatgaa ggtccgcctg ccagacacgg aatggtcgat cgagattccg
      361 ccggggctgc caccggtcga gggcgatccc tcgctgctgc agcaagtgct gcggaacctc
      421 tatgaaaacg cgatcaaata caccacgggc cacgcattca tccggacgac ggcggaagtc
      481 gacgaacatg gcgtcgagat ccacatcatc gacaacggga tcgggatcgc ggctgagcac
      541 gtgccgttcg tgttcgagcg attccggcgg cccggggccg atcccgccgt tcgcgggatg
      601 ggactcggtc tttacctgtg ccacaacctg atgacggcgc aaggcggacg gatccgggtc
      661 gaatcccacg ggatcggaat gggcacgacc ttctcgatcg cgcttcccat cgccagtggc
      721 tgggacgccg acgaatgaat tcagtgagat gcaaaggacg ggcgcaatgc gcaaacggtt
      781 gatcctcatc gtcgacgacg agcccccgat cgcgcgtctc tcccgcgcca aactcgaggc
      841 cgatggctac gccgtcgtac tcgcgacgaa cggcgaggag gcgctccgcc ggatcgagga
      901 ggatcgtccc gacctcgtgg tgctcgatgt catgatgccc gtcatggatg gtttcgaggt
      961 gcttcgccgc ctgcgggcga cgagccagct tcctgtgatc ctgctgacgg cgcgcacgag
     1021 cgacatcgac aagctgaccg gccttcatgg tggcgccgac gactacctga cgaagccgtt
     1081 caatccggac gaactctccg cccgcatcgc ggcgatcatg cggcgatccg ccggcgtcgg
     1141 catcgtcacg aaccatcagc tcatgacgta ccccgcgaca ggcgtattgc ccgaggtcac
     1201 gatcgacatc gagcagcggc ggatcatggt tggcggcgcc gaggtacggc tgagccgcac
     1261 ggaatgggag ctcctgacgc agctcgcggc gaactccggc cgcgtgatgc tccacggcga
     1321 actgttgagc cggatctggg gacccgagtt ccgcgacgag gtgcaatacc tccggacctg
     1381 gatcagccgg attcgcacca agctggaacc cagtgcaagc gacggctcgc gcatcatcac
     1441 gaccttcccc ggtctcggct atcggatgga atctcccgtt gcaatgccac cggcagagta
     1501 gcccgctctc agttgccaac gttccagacc gtctgcagcg cccgatagtc gagaaggacg
     1561 atccccaact cgtcgatcag atcacgcgct tcgggcgacg tgacgaaatc atggtccgcc
     1621 cgccggatct gccaggtctc gggttcgatg gcccgggatt cgtcatcacc gaggctggga
     1681 tgcaccgccc actccgtgag ccccggcggc agcgcacgca ggagcgccgc gaaacggttg
     1741 gccttgtcct cgaaacccat gtgatagcta tccagcacgt cgtgatcgac gacgggaagc
     1801 ccctcacgcc ggagtcgctc cgcatgaccg ctccgatgga ttcgcatcgc gacgccgtat
     1861 tcgcgagcga ggcggtagca gagatcgaag atatcctcgc gaccaccgtc ggcgatgcag
     1921 tgccagtcga gatgcgacgg cgtgagacca gcggcgaggg ccgcctcgat ctgcgcccga
     1981 aactcgatct ccacctcgcc aatgtcggcg cgggcgagga gatcgggaat ctggtcgtag
     2041 acgggaaagt aacccgacgc gtcgatcagc gaagcgatcc gatccttcga tgccaccgga
     2101 ccccatcggt atccgccgaa ttcgctgacg agcgtcagat gcacggagaa ggggagttcg
     2161 ggatgatcgt cgaggatccg catcgcatcg ggcgcccatg cgcacggcac catcaggctg
     2221 gtcgaggtga tgatcccgcg cgtgaatccc tgcaggacag cttcattcac ggcgtgacac
     2281 atgccgaagt cgtcggcatt cacgatcagc aggcgcgcgt catccgcata gccgaggagg
     2341 gcattggtac tgcgctgcgt caaggattgt cctcccgtac aaggttcgat tggcgagccg
     2401 cgtcgtcagg aggatcgcag gttcacgaca aacgcgcgtc cacgtgcccc gcggtgagtg
     2461 atgccgcctc gtcgatccag gcggtgagtg ctccttgttg cgattccatc gggaggtcgc
     2521 cgagcatccg gcgaatctcc tgcgatgtca cggtacgcag gatcgcgatg agttggatcg
     2581 ccatcaagcg gacgcgcggc gagggtgatg cctcgccggc ttcgcgcgcg agttcggcgg
     2641 tcagcgtatc ctcgtagtca gcccacatcc ggaggagtcg cgattccagc acgagactgt
     2701 cgccgacacc gcggtgaaag ccctcgacac cgagtcccgg tcggtcgggg tcggctacga
     2761 gtcgcttgag tgtccccgcg accgcatcga ccggagaaat gccgggatcc cgcgcgcgga
     2821 gatcgtcgac gatggcatcg atcagccagt ccgcctcacc aaacgcgaga tcctccttcg
     2881 accggaaata ggtgaagagg gtcttcaccg agacattggc gccatcggcg atttcggcga
     2941 cggtgacgtt gtcatagcca cgctcctcga agagggcttc cgccacactc aggatcgtgc
     3001 tcctggtcat gattttcttg cgttcgcgga gcccgggtgc ggcagatggc gtcgtcatgg
     3061 gtgaagctta atccagaatc gtccagaacg tatattcatc ccccatgcgg accccggctt
     3121 cggaaactgt cacgccaggc gagcggccat cgcgagcaaa tcctcatccg ggttcgtgtt
     3181 tctcaccgca cgtaggcaat cctggacgca ggacgcacga cacctacgcg acaacccttc
     3241 tgatccgcca ggagccaatc gcggcaaaga tgatggcgat cgcggccaga acggcggcga
     3301 tcagcgatgc cgcggcgtgg tgaccggcga gggagatcga tatgccgtag ttcgcccgca
     3361 ccgtggcagc cgcctggggt tggccatgcg tcaacgcggc gagactccgc gacgtaagcg
     3421 gtccgcgcac cagcatcgcc gcctccgcga acgggagcca gttcagtacg cgcacgacgc
     3481 catgcggaag ggcttgagcc gggatgtagg ccatcgcgag gaacccggcg aaggtcccga
     3541 cgatcgcgct gagcgatgag aaggcgccac tcgtcgccgt gaacgtaacc gcgagacccg
     3601 agattgccga gaacgccgcg cacatcacga tcacggaccc cacgcagcgc agccaatccg
     3661 cggggtgcat cagcgacgca cctgtcacga cggtgatgag ctgacttgcc acaagcacga
     3721 acaggctgat cgagagggcg atcaggaagc tcgtgagact gtagccgagg atcagatcgc
     3781 gtcggcggat cggggagacg acgaaatcgg cgaagcggcc gctcgcgcgg tcgtcgacga
     3841 agaccgtcat cgccccgagc ccggcggtca acgtggtaag catcagcgtt cccgcgaaga
     3901 cccaggcatc gacaaaatgc tgggcatcgt cgtgattggc agctggccag tcgtccttga
     3961 gattattgac ctgaatattc cccagaaaca gcttgtagag cgcgaagagg atcaacggcg
     4021 acagaagcgc gaagaagacg cccatccggt cgcggaagaa aatccgtaga tgccggcctg
     4081 tcagagtcca gatcattcga ctctcctccg accagaggtt gggtcgacca gtgagaggaa
     4141 gacatcatcc atcgtgccgt ggcgaaattc gaaatcgacg agttccgcct cgtgacgctt
     4201 gagcaacgtc aaggcatcct gactcgagcc gacaccgatc cgaagcacgt ccccctgctg
     4261 gctgaagacg tacccgcgcg cctcgagatc ctggcgaatc gcatccgcac tccgcgccac
     4321 cagttgcagc tcgtcccggg cgtgcgccgc gcgaagcgcc gtcggggttc cctcggcgac
     4381 gatcgtcccg tggtc
//
LOCUS       DASTMD010000265         4159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_828234, whole genome shotgun sequence.
ACCESSION   DASTMD010000265 DASTMD010000000
VERSION     DASTMD010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828234"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..948)
                     /locus_tag="VFQ54_03990"
     CDS             complement(361..948)
                     /locus_tag="VFQ54_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEU0164180.1"
                     /translation="MGDENQAGSAAGSAAGSAVVIAVSIDGDHRFSKGTRERIRLLEG
                     FGVEGDTHAGSTVQHRSRVARDPSQPNLRQVHLMHAELFDELAAQGFNVQPGDLGENV
                     TTRGLDILALPTGSRVRIGDSAVVEITGLRNPCGQIENFQAGLLKAVLDRDTDGNLVR
                     KAGVMGIVLDGGEIRPGDPISVDLPAGPHVPLAPV"
     gene            complement(1060..1929)
                     /locus_tag="VFQ54_03995"
     CDS             complement(1060..1929)
                     /locus_tag="VFQ54_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU0164181.1"
                     /translation="MELPEEPSVPNDVPAVRLAPGQWVSPTLLNTYAICPYRVRLQYI
                     DRVLAPPRYDHFLVQGSLAHSLLKHAAQLIARRQPEPSPEKIYEMAIRRLHERNFPTV
                     EAMEGQARQITRWVRTGIAHLDRKADYLLIEKPRHRPFNKRDPDSDITMTFRPDLVLQ
                     REDDAGSFIEVIDYKTGKRREESDVPVISRFILNSLLEEQMPNAWQMRVRFTFIWLDI
                     GEVETIDLDAAYCSKRWAGILRDIDRLLNETGWKPAPSILCHYCPFHENACTAYVPGL
                     SDGNPVPAFHGGD"
     gene            complement(2175..2600)
                     /locus_tag="VFQ54_04000"
     CDS             complement(2175..2600)
                     /locus_tag="VFQ54_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164182.1"
                     /translation="MDRVRWSPLATAMIALAVAVLVAVHILGAESGVVIFNFGLWMTV
                     CVLGVAFLASLRPTSVTREGLFVLANGLGLSIGLLAIGFLGVSSMLGLTPVLAGIAIT
                     AWPRWPGQPLLTPVNRVVLVGGACVFPVLALSQWIFGIS"
     gene            complement(2737..2958)
                     /locus_tag="VFQ54_04005"
     CDS             complement(2737..2958)
                     /locus_tag="VFQ54_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164183.1"
                     /translation="MGRFPIRTGDFDPDEIRRYVAHIRWPISRDDLLADLRERGIDQR
                     VIDPVIDNTAARHFVSADDLVAKLEGLDA"
     gene            3149..3526
                     /gene="crcB"
                     /locus_tag="VFQ54_04010"
     CDS             3149..3526
                     /gene="crcB"
                     /locus_tag="VFQ54_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU0164184.1"
                     /translation="MQYLWVGVGGFVGANLRYILGRIISDRWGSSFPYGTFVINVTGA
                     FVIGILMVILTERTVADPLWRQLTVVGFLGGYTTFSSYTWEAVQLFDDGRWGPGLGYL
                     IGSNLLGLLACAAGMIIARNVTL"
BASE COUNT          738 a         1383 c         1334 g          704 t
ORIGIN      
        1 cggatatcga ggatcccgca cccttcgatc ccaccaatcc cactcccgtt cccgccgcga
       61 ccagcatccc accggtcgcg acgacggtcc ccatcccgac cacggctcct cgcgcaacga
      121 cggccccaca ggttccagac gatcccgccg caccatcggc gccgccgcca acgtctgcac
      181 catccgcgcc ttcgggtgat aacaccgcgc cgccggcgtc aaccacagcg acggcacgga
      241 ccagcgacgt gaaggtggca gcccttccca acacgggcac gggcgtcatc ccgccggcag
      301 ccggatggcc cctcgctatc gcgttgatct cgggaattgc ggcgacgctc ggcctcgtcg
      361 tcagacgggg gccagcggga cgtgcggtcc ggcgggcagg tcgacgctga tcgggtcgcc
      421 gggtcgaatc tcgcctccgt cgaggacgat tcccatcaca ccggccttgc gaacgaggtt
      481 tccatcggtg tcgcggtcga ggacggcctt gaggagcccc gcctggaagt tctcgatctg
      541 cccgcaggga ttgcgcaagc ccgtgatctc gacgacggcc gaatccccga tccgcacccg
      601 cgatccggtg gggagcgcga ggatatcgag accacgcgtc gtaacgttct cgccgagatc
      661 gcccggctgg acgttgaatc cctgcgctgc gagttcatcg aatagttcgg catgcatcag
      721 atgcacctgc cgcaagttcg gctggctcgg atcgcgcgcc acccgcgatc gatgctgcac
      781 cgtggacccg gcgtgggtat ccccctcgac cccgaatcct tcaaggagcc ggatccgctc
      841 ccgggttccc ttgctgaagc ggtgatcgcc gtcgatgctc accgcgatca cgaccgcgct
      901 gcccgcggcg ctgcccgcgg cgctgcctgc ctggttttcg tcgcccattc gacctgaatc
      961 cctcgtcgct ccccgccgcc tgcatcacgg cgaatccatc gccaatcgct tccgattgta
     1021 gccgcagccg gggtcgacga ccgctcgcgg gcatcaggat caatctccac catgaaaggc
     1081 ggggactgga ttgccatcgg agaggccggg aacgtaggcg gtacacgcgt tctcgtggaa
     1141 ggggcagtag tggcagagga tcgatggcgc cggtttccac ccggtctcgt tgagcagccg
     1201 atcgatatcc cggaggattc ccgcccaccg cttcgagcag tacgcggcat cgagatcgat
     1261 cgtctcgacc tcgccgatat cgagccagat gaaggtgaag cgaacgcgca tctgccaggc
     1321 gttcggcatc tgctcctcga gcagggagtt caggatgaag cgggagatga cgggaacgtc
     1381 ggattcctcg cggcgctttc ccgtcttgta gtcgatgacc tcgatgaacg acccggcatc
     1441 atcctctcgc tggagcacga gatcgggacg aaaggtcatg gtgatatcgg aatccggatc
     1501 ccgtttgttg aacggccgat gacgaggttt ctcgatcaga agatagtccg ccttgcgatc
     1561 gagatgggcg attccggttc gcacccagcg cgtgatctgg cgcgcctggc cctccatcgc
     1621 ttcgaccgtc gggaagttcc gctcgtgaag ccgcctgatc gccatctcgt agatcttctc
     1681 gggggatggc tccggctggc gcctggcaat gagctgggcg gcatgcttca gcaggctgtg
     1741 agcgaggctt ccctgcacga ggaagtgatc gtacctgggg ggcgccagca cgcggtcgat
     1801 gtactggaga cggacgcggt agggacagat cgcgtaggtg ttgagcaggg tcggcgagac
     1861 ccattgcccg ggcgcgaggc gcactgccgg cacatcattc ggtacggatg gctcttccgg
     1921 gagttccatc gctgcccacg cctggattgg tcggttggtc taccagtgtc ccacctcccg
     1981 agcatacgcg gtttgcgcaa cgattgcatc aattgacgtg caccgatttc acgaaaccac
     2041 gcttcccgct ccgggatagt gccgggtcat tccaccacga tcccacagga gtgaccacaa
     2101 ggatccccgg ttcaggggag gaccacgcct gactgaaacc ggttcccgat cgacgagatg
     2161 ggaaccgcgt tcgttcagga aataccgaag atccactggg aaagtgcaag caccgggaag
     2221 acgcaggctc ccccgaccag cacgacgcgg ttgacgggtg tcagaagcgg ctgccccggc
     2281 cagcgcggcc aggccgtgat cgcgatcccg gcgaggaccg gggtcagtcc cagcatcgaa
     2341 ctcacgccga ggaatccgat cgcgagcaag ccgatgctca ggccgagccc gttcgcgagc
     2401 acgaagagcc cctcccgcgt gacggaggtc ggccgcaggc tcgccaggaa ggcgacgccg
     2461 agcacgcaaa cggtcatcca gagtccgaaa ttgaagatga ccacgcccga ttccgcacca
     2521 aggatgtgga cggcgacgag caccgcgacg gcgagcgcga tcatcgcggt ggcgagtggc
     2581 gaccagcgaa cgcggtccac cggctcgcct tgccctccgg ccgctgcctg cgatgggcct
     2641 gcttcggtta cggggacagc gggggaaccg gcggtcggca cggtaatcac atgtcggggc
     2701 ggggaatcgg acacaggcct tcaatcacga acggggttac gcgtccaggc cctcgagctt
     2761 ggcgacgaga tcgtcggcgc tcacgaaatg ccgggcggcg gtattgtcga tcacgggatc
     2821 gatgacccgc tggtcgatcc cccgctcgcg gagatcggcg aggaggtcgt cccgcgagat
     2881 cggccatcga atgtgcgcga catagcgccg gatttcatcg ggatcgaaat cgccggtgcg
     2941 aatcgggaag cgccccatga ctggacgatc ctttcgggcg tgagcgcatc cggggctccg
     3001 cacgagcacc gacgccgcca tctaccgttc tgagtatcgg gtcttcgccc atgtcgcgcc
     3061 accggcatcg cccgccattg tgtgaacgga cgcccggcct ataatcgccg gaaagaacgt
     3121 cacgacgatc gtatgggcga ggtcaggaat gcagtatctg tgggtagggg tcggtggttt
     3181 cgtcggtgcg aatctccggt acattctggg tcgcatcatc tcggaccggt ggggatcgtc
     3241 attcccttac gggacgttcg taatcaacgt caccggcgcc ttcgtgatcg gcatcctgat
     3301 ggtgatcctt accgagcgga ccgtcgccga tccactctgg cggcaactga cggtcgtcgg
     3361 cttccttggc ggctacacca ccttcagcag ctacacctgg gaggcggtcc agctcttcga
     3421 tgatggccgg tggggaccgg gtctgggcta cctgatcggc agcaatcttc tcggattgct
     3481 cgcttgcgcg gcgggaatga tcatcgcgcg gaacgtcacg ctctagcgga ttcgagcctg
     3541 taaaccttca cggcttcttc catagaaata gtggcgtgcg gaagtacgtc cccgactgcc
     3601 aaccgctcgc ctggaacgtc cagccttcgg gccccggaac caccccctca tgacactcat
     3661 gacacgtcct cccgagcctg tcgagggacc agccggcgtc cacgtgcgtc ccgtacgacg
     3721 ccgcacacaa cacgtcttcc cgagcttgtc gagggatcag ccggaatcca cgtgctcccg
     3781 gcacgacccg tcgccagccg gggtggaacg gggagcatcg cgcgtgggac gtggtcccgg
     3841 gcgaacacca ggttcgcccc tacggatgcg cgcctcacgg cgctccgccc gggataacgt
     3901 gacggggttg ccgccgcggg acatgtatcc aacgaccagg gcgaacacca ggttcgcccg
     3961 gatttgcgac cgaacggtgg caagatcgat ccacccggat caggagaggt gggccgcaac
     4021 cgcaaggtcg cgcgtcagct ccgcctggcc gagatgctcc gccgcgtggc gcgccaccac
     4081 gatcagaatg tcccggcccg tcatctcacc gcgccgcggg tgcaggtatg tcgcctccag
     4141 ggcgtcgggc tcgagcgcg
//
LOCUS       DASTMD010000266         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_916550, whole genome shotgun sequence.
ACCESSION   DASTMD010000266 DASTMD010000000
VERSION     DASTMD010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916550"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(471..2498)
                     /locus_tag="VFQ54_04015"
     CDS             complement(471..2498)
                     /locus_tag="VFQ54_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0164185.1"
                     /translation="MSTERRRFTRRGFVATSAISTASAATVGVAVLGGPGKAVFAQTT
                     DEATPVPLPDGPITDVTIDTGDWRSTNGDNTGQRAAKTKIDGSNVTTLGPAWRIDLTD
                     ADGKPAAVTGTPIIVGDLVYIQDMNSNVTAYDRASGKVKWAKTYNLGCNGPNGVTYGY
                     GNLFFGLGESAEVAAINAKTGDDVWRVKLSNMKEEGIRMSPVAYGGIVYVSIVPLSTN
                     NNPGSHGILHALDAQTGNTIWYFDLSVDNLWGNARKNMGAGLWYAPNFDEKGNLYFGN
                     GNAAPWPGDEQDPEGGTRHNANLYASTAMSVDPTDGTVRWYFQPQPFDLTDGDYQIAP
                     MIVQATINGVDTKVAICAGKTGDVVAIDADNGNMLWWTKVGKHENDLLQELTDEYIQV
                     FPGGNGGVLTPPAYADGVYYCVITNRPSWVNATGSESHPEDPFEGEVIAIDVPTGNIL
                     WDVYVPTFPTGSIIVANDQVITAGLDGLIRAFNRKDGTQSWNFQLKAGVNAPVAIAGD
                     ELYVAAGTQYEPAPDQITEQVAATATYELIKLTLGPGGGVVPSPAAPKINAAQASDAT
                     PAATAANVAPVTPEADSKGVLRILVEAGDLYFKPNALAIPANNDVKLTVRNVGALQHD
                     FVIEKPPVDSGMIASGNSVELNLNLAPGTYQFLCTVEGHADAGMIGTITAE"
     gene            complement(2823..>3241)
                     /locus_tag="VFQ54_04020"
     CDS             complement(2823..>3241)
                     /locus_tag="VFQ54_04020"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU0164186.1"
                     /translation="TVDSPAGTVFEIGAHVAVGGLDDLACSGDVFLASLAACQELTIR
                     LVAASMGIELKRLHLRVEGDWDVRGTLAVSRESPIGFTAIRILVDLDVDAPADRIERM
                     LRSAERFCVVSATLKDAPAIQLIPTIGGEAIPTAVA"
BASE COUNT          542 a         1011 c         1060 g          628 t
ORIGIN      
        1 tcgtgccgtc agcgccgttg gaggaaccgt ggacgcggcc cttgtggccg cctcggatcg
       61 gctgcgccga tccggtcccg gagaacgccg tgtggctggt gctccctcgg agtccggctg
      121 cgccggatgt gcttcgcacg gagatttctc cattccgccc cgcttcagtc gaaatgacag
      181 gcttttatgc gacagcgttg gcgggacagg tcgtccggac tgcggtccgg cagcggcggc
      241 aatgtcccgc cctacgaacg accgacttcc ttgtggaacg ccgcaaccga tccgacggca
      301 ggtagagcac ccgtttcggc tttccgcgtg gacgacggca agccatgtgg ccaattgctc
      361 acagggtgtg cgacgtcgag aaggatcgcg cgatttcagg gcacacgaag acagggcggc
      421 aatgagccgc cctgtccccg ttcgcatgct ggagcgacgt ttcctgcggc ctactcggcg
      481 gtgatcgtgc cgatcatgcc ggcgtccgca tgaccctcga ccgtgcagag gaactggtag
      541 gtccccggag ccaggttcag gttcagctcg acgctgtttc cgctcgcgat catgccggaa
      601 tcgaccggcg gcttctcgat cacgaagtcg tgctgcaggg cgccgacgtt gcgaaccgta
      661 agcttgacgt cgttgttcgc cggaatcgcg agagcgttcg gcttgaagta gagatcgccc
      721 gcttcgacca gaatgcggag gacgcccttg ctgtccgctt ccggcgtgac cggcgcgaca
      781 ttcgccgccg tggccgcggg ggtcgcgtca gacgcctggg cggcgttgat cttcggcgcg
      841 gcgggagacg gaacgacgcc gccacccgga ccaagcgtga gcttgatcag ctcgtaggtt
      901 gcggtggccg cgacctgctc cgtgatctgg tccggtgccg gctcgtactg cgtaccggcc
      961 gccacgtaca gttcgtcacc cgcaatcgca accggcgcat tgacgccggc cttgagctgg
     1021 aagttccagg actgcgtgcc gtccttgcga ttgaaggcgc ggatgaggcc gtcgagtccg
     1081 gcggtgatca cctggtcgtt ggcgacgatg atcgatccgg tcgggaaggt cgggacatag
     1141 acatcccaga ggatgttgcc ggtcgggaca tcgatcgcga tgacctcgcc ttcgaagggg
     1201 tcctccggat gtgattccga cccggtcgca ttcacccagc tcgggcggtt ggtgatcacg
     1261 cagtagtaga cgccatcggc ataggccggc ggcgtcagca cgccgccatt gccacccggg
     1321 aagacctgga tgtactcatc ggtgagttcc tggagcaggt cattctcgtg cttgccgacc
     1381 ttcgtccacc agagcatgtt cccgttgtcg gcgtcgatcg cgacgacatc accggtcttg
     1441 ccggcgcaga tcgcaacctt ggtgtcgaca ccgttgatcg tcgcctgcac gatcatcggg
     1501 gcgatctggt agtcgccgtc ggtgaggtcg aacggctgcg gctggaagta ccagcgcacc
     1561 gtgccgtccg tcggatcgac cgacatcgcg gtgctggcgt agaggttggc gttgtgacgc
     1621 gtgcccccct ccggatcctg ctcgtcaccc ggccacgggg cggcgttgcc gttaccgaag
     1681 tagaggttgc ccttctcatc gaagttcggg gcgtaccaga gacccgcacc catgttcttg
     1741 cgagcgttgc cccagaggtt gtccacactg aggtcgaagt accagatcgt attgccggtc
     1801 tgggcatcga gggcatggag aatcccgtgg gagcctgggt tgttgttcgt ggagaggggc
     1861 acgatcgaga cgtagacgat gccgccatag gcgaccggcg acatccggat gccctcttcc
     1921 ttcatgttgc tgagcttgac gcgccagaca tcatcgccgg tcttggcatt gatcgcggcg
     1981 acttcggcgg actcgccgag cccgaagaag aggttgccgt acccgtaggt cacgccgttc
     2041 ggcccgttac aaccgaggtt gtaggtcttc gcccacttga ccttgccact cgctcggtcg
     2101 taggcggtga cattgctgtt catgtcctgg atgtagacga gatcgccgac gatgatcggc
     2161 gtgccagtca ccgcggccgg cttgccgtcc gcatcggtga gatcgatccg ccaggcaggc
     2221 ccgagcgtgg tgacgttgga gccgtcgatc ttcgtcttcg cggcgcgctg cccggtgttg
     2281 tcaccattgg tcgagcgcca gtcaccggta tcgatggtga cgtccgtaat cggcccgtcc
     2341 ggcagcggca ccggtgtcgc ttcgtccgtc gtctgggcga aaacggcctt ccccggtccc
     2401 ccgaggacgg ccactccgac cgtggcggct gaggctgtgg agattgcgga agtcgcgaca
     2461 aagccacgac gtgtgaaacg acgtcgttcc gtagacatgg tctatccctc tcttggggag
     2521 gagcgcccca taactgtatg caaacggtga accgtggttc ggtactcgcg cgcgcgctca
     2581 catagtccca cccggcatca ggatgtcaat aggtttgggt tcgattaata tttgttcaga
     2641 tactttcaat tgtagccgac gctggcgaat ctatcagatt gagtctcgtt ctcaaccttg
     2701 acgagacggc gtcccggctg atgtgggacg cggaaaagtc gcccccagga gagagcgact
     2761 caccagattc gccattcgtt tcccacgtga ttgccgcgaa tggatcgggc caccgtgtcg
     2821 gattacgcga ctgcggtcgg gatcgcctcg ccaccgatcg tcgggatcag ctggatcgcc
     2881 ggcgcgtcct tcagcgttgc cgagaccacg cagaatcgct ccgccgagcg caacatccgc
     2941 tcgatcctgt cggccggggc atcgacatcg agatcgacga gaatccggat cgcggtgaag
     3001 ccgatcggtg attcgcgcga aaccgccagt gtgccccgga catcccagtc gccctcgacc
     3061 cggagatgga gtcgcttgag ctcgattccc atcgacgcgg caacgaggcg gatcgtcagc
     3121 tcctggcagg cagccaggga tgcgaggaag acatcgcccg agcaggccag atcgtcgagc
     3181 ccacccaccg cgacatgggc gccgatctcg aagaccgtcc cggcggggct atcgacggtg
     3241 a
//
LOCUS       DASTMD010000267         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_916566, whole genome shotgun sequence.
ACCESSION   DASTMD010000267 DASTMD010000000
VERSION     DASTMD010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916566"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1705
                     /locus_tag="VFQ54_04025"
     CDS             <1..1705
                     /locus_tag="VFQ54_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEU0164187.1"
                     /translation="AFPICVSTTDPGEIVETGIAIAPTFGAICLDDISSPRAFTIADN
                     LDKGADIPVFSNQHHGTAILVLGALHNALKVVGKKLDEINVVLSGAGIAGIGVARLLT
                     RAGVKNLVVCDRAGAIHRYRSERMNWAKSYVAKETNLDQRQGSLAEMLKGADVFIGVS
                     TGDIVTEEMIRAMAPDPIVFALAVPTPEVLPAVARAAGARVIATGRSDYPNTMDVSLV
                     FPGFFRGLLDSGSRNIRLRMLLYAARALADMVPESELHEDNIIPQLFDFRVAPAIAAA
                     VVRGAIEADEATRDITPEHVAERTRRYVYEGRLDAPQPSTRLEGRTFREEAIDLRVRN
                     HGILEIRSKIPIRDHHILNLLYVPPRAFVPAHVIRDDISRVTELTGKGNLVAIVTDGS
                     AVLGLGDIGPQAGLPVMEGKAVLFQTLAGVEAFPICLAARDPDEIVRLVTAIAPSFGG
                     INLEDIGAPRCFEIEEKLRAALDMPVFHDDQHGTAIVVCAGLINAARLRDGQIADMKV
                     VINGAGAAGIAVAKLMHVLGAADVILCDRTGAIYTGRPGRMDASKQAIAEITNREGKT
                     G"
     gene            1715..2206
                     /locus_tag="VFQ54_04030"
     CDS             1715..2206
                     /locus_tag="VFQ54_04030"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEU0164188.1"
                     /translation="MIRGAEVFIGLSAPGSLTPEMVRSMAPNPIVFALANPTPEIMPD
                     LAIEAGAFAIATGRSDYPNQINNSLAFPGVFRGALDVKARGISDDMKIAAAQAIADLV
                     PADKLTPEFLIPDSLDLRVSPRVAAAVARSAQETGLARLMTDPAEIEARCRDLVYEGT
                     VAL"
     gene            2301..3470
                     /locus_tag="VFQ54_04035"
     CDS             2301..3470
                     /locus_tag="VFQ54_04035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0164189.1"
                     /translation="MTTTDEIRILPNHDGGENTRIELLIDGTPVSWCWIVPMTLRIGR
                     AVVRMDGIGGVGTGETKRNRGYSSRVLVAAVEEMKRGDAPLTTLYGIPDYYPRWGYAT
                     IGHEGDHRLVRLDADNVLPEGFSWRFATPADLPGIRALYDEQAPLATGAAVRGPATPS
                     STQLAKSVETDDKSCVVVIDDRDDVVAYAWMAGYIWWMERVAKPRDPDALLIGEAFAD
                     GSVAADALLAALKQWATELEKPHVAIHQPPIGDLGLAARLQDVEFHGRSFRAAQFMGR
                     STGIGGLMTALAPELSARWLGSRSPWTGSLLIRTPEDEVLLGIDRGGVRVLDDLAGSD
                     ATLIELTVGDVARLAFGAFPPRDLLARLGISDDVADVLAHLFPEGHPYIYPADRF"
BASE COUNT          604 a         1199 c         1179 g          615 t
ORIGIN      
        1 cgccttcccg atctgcgtat ccaccactga tcccggcgag atcgtcgaga cgggaatcgc
       61 catcgcgccg acgttcgggg cgatctgcct cgacgacatc tcctccccgc gcgccttcac
      121 cattgccgac aacctcgaca aaggggctga cattccggtc ttctcgaatc agcatcacgg
      181 cacggcgatc ctcgtgctgg gggcgctgca taacgcgctg aaggtcgtcg gcaagaagct
      241 cgacgagatc aacgtcgtgc tcagcggcgc cggaatcgcc gggatcgggg tcgcccgcct
      301 gctgacgcgc gctggggtga agaatctcgt cgtctgcgac cgggccgggg cgattcatcg
      361 ctatcgatcg gaacggatga actgggcgaa gtcgtacgtc gccaaggaaa cgaacctcga
      421 ccagcgacag ggctcgctgg cagagatgct gaagggagcc gatgtcttca tcggcgtctc
      481 caccggtgac atcgtcaccg aggagatgat ccgggcgatg gcgccggatc cgatcgtctt
      541 cgcgctcgcc gtgccgacgc cggaagtgct gcctgcggtc gcccgtgcgg ccggcgcccg
      601 cgtcatcgcg accgggcgct ccgactatcc gaacacgatg gacgtctcgc tcgtcttccc
      661 cggcttcttc cgcggtctgc tcgacagcgg atcgcggaac atccgcctgc gcatgctgct
      721 ctacgcggca cgagcgctcg ccgatatggt gccggagtcc gagctgcacg aggacaacat
      781 catcccccag ctcttcgatt ttcgggtggc gccggcgatc gccgccgcgg tcgttcgtgg
      841 cgcgatcgag gccgacgagg cgacgcgtga catcacgccg gagcacgtcg ccgagcggac
      901 gcgccgctac gtctatgaag gacgtctcga cgcgccgcaa ccgagcaccc gccttgaggg
      961 gcgcacgttc cgcgaggaag cgatcgacct tcgtgtccgc aatcacggaa tcctcgagat
     1021 ccggagcaag atccccatcc gcgatcatca catcctcaac ctgctctatg tgccgccgcg
     1081 agccttcgtt ccggcgcatg tgatccggga tgacatttcc cgtgtcaccg agctgacggg
     1141 gaaggggaac ctcgtcgcga ttgtcacgga cggttccgcc gtgctcggac ttggcgacat
     1201 cggaccacag gccggactgc cggtgatgga agggaaggcc gtgcttttcc agactctcgc
     1261 cggcgtcgag gccttcccga tctgtctcgc cgcgcgggat cccgacgaga tcgtgcgcct
     1321 ggtgacggca atcgcgccgt cgttcggggg tatcaacctc gaggacatcg gggcgccgcg
     1381 ctgcttcgag atcgaggaga agctgcgtgc ggcgctcgac atgccggtct tccacgatga
     1441 ccagcacggg acggcgatcg tcgtctgcgc gggcctgatc aacgcggcgc gactccgaga
     1501 cggccagatc gccgacatga aggtcgtcat caacggtgcc ggcgcggcgg gcatcgccgt
     1561 cgccaagctg atgcatgtcc tcggcgccgc cgacgtgatc ctctgcgacc ggaccggtgc
     1621 gatctacacg ggacgaccgg ggcgcatgga cgcctcgaag caggcgatcg ccgagatcac
     1681 caatcgcgag ggcaagaccg gctagctggc cgatgtgatc cggggcgcgg aggtcttcat
     1741 cggtctctcg gccccgggct cgctgacgcc ggaaatggtc cgctcgatgg cgccgaatcc
     1801 gatcgtcttt gcgctggcga atccaacacc ggagatcatg cccgatcttg cgatcgaggc
     1861 cggtgccttc gcgatcgcga ccggacggag cgactacccg aaccagatca acaactcgct
     1921 tgccttcccg ggtgtcttcc ggggggcgct ggacgtgaag gcacgcggga tcagcgatga
     1981 catgaagatc gcggccgcgc aggcgattgc cgatctcgtt ccggccgaca agctgacgcc
     2041 ggaattcctg atcccggaca gtctcgatct ccgcgtctca ccgcgcgtcg cggcggccgt
     2101 cgcccgctcc gcccaggaga ccggtctcgc ccgcctgatg accgatccgg ccgaaatcga
     2161 ggcacgttgc cgggatctcg tctacgaggg caccgtcgcc ctgtaaccgg caatcccgta
     2221 tcatcgcaat ctatctggag acgaccggcc gacctgatgt cagccggtcg tcgcgtcaac
     2281 gaggggagac ggtgagcgcg atgacgacga ccgatgagat ccggatcctg ccgaatcacg
     2341 acggcgggga aaatacccgc atcgaactcc tcatcgatgg gactccggtc agctggtgct
     2401 ggatcgtgcc gatgacgctc cggatcggcc gggccgtcgt gcggatggac ggcatcggtg
     2461 gcgtgggaac gggcgagacg aagcgcaatc gcgggtattc cagccgtgtc ctcgtggcag
     2521 cagtcgagga aatgaaacgc ggcgacgccc cgctcacgac gctctatggc atccccgact
     2581 actatccgcg ctggggatac gcgacgatcg gtcacgaggg cgaccatcgt ctcgttcgcc
     2641 tcgacgcgga caatgtcctt cccgaaggct tttcgtggcg cttcgcgact cccgccgatc
     2701 tacccgggat ccgcgccctg tatgacgaac aagcaccgct cgcgacaggc gccgccgttc
     2761 gcggaccggc gactccctcc tcgacgcaac tcgcaaaatc cgtcgagacg gatgacaaga
     2821 gttgtgttgt cgtgatcgac gacagggatg acgtcgtcgc atacgcgtgg atggcaggct
     2881 acatctggtg gatggagcgc gtcgcgaagc cgcgagatcc cgatgcattg ctgatcgggg
     2941 aagccttcgc ggacggatcg gtcgcggcgg atgcgcttct cgcggcgctc aagcagtggg
     3001 cgaccgaact ggagaagcca cacgtggcga tccatcagcc tccgataggt gatctcggac
     3061 tcgcggcccg cctccaggat gtcgagttcc acggtcgctc gttccgcgcc gcgcagttca
     3121 tgggacgctc gaccggaatc ggcggtctga tgaccgcgct ggcaccggag ctcagcgccc
     3181 gctggctcgg ttcgcggtcg ccatggacgg gttcgctcct gatccggacg ccagaggacg
     3241 aggtcctgct cggcatcgat cgcggtggcg tccgcgttct cgacgacctc gcgggttcgg
     3301 atgccacgtt gattgaactg accgtgggag acgtggcgcg acttgccttc ggggcatttc
     3361 ccccgcgcga tcttctggcg cgtctgggaa tcagcgacga cgtcgcggat gttctggcgc
     3421 acctgttccc ggagggacat ccgtacatct atcccgcgga tcgtttctga cgcgagcacc
     3481 aacagccggg ccacgaccgc gcgccttcga tatcgccacg ccgacggtca tatccggttg
     3541 cgccggatgg gccacgccct tcggctgcgc tcgcggcagg ctccggtggt gaccccc
//
LOCUS       DASTMD010000268         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2078116, whole genome shotgun sequence.
ACCESSION   DASTMD010000268 DASTMD010000000
VERSION     DASTMD010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078116"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VFQ54_04040"
     CDS             complement(<1..886)
                     /locus_tag="VFQ54_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164190.1"
                     /translation="MTTANRTAFQIAFEKWSEDEIRHLVASLTRRRFVGTAATIAALA
                     GLPAAARAQTPVASPVGGTRTVDTVNGPVEVPANPTRVVCIDSYSITALIDAGLTPIG
                     IPAIDLDSYISVYKDALVKVPTVGSYQGIDVEKIKTLNPEVILAINAPWATSVYDQLK
                     EIAPTVVFDYAVPNAWQVLADEFTGAIGRTDELDVVKKTYADATNTMKTTYADQLASL
                     TWAVANGWGSADNQYTLYFPDSAPGVVLSDSGVTWIDPVTGKTGTSELISYERLDQLV
                     EADIILTYGERDGTPNADS"
     gene            complement(1105..>2062)
                     /locus_tag="VFQ54_04045"
     CDS             complement(1105..>2062)
                     /locus_tag="VFQ54_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164191.1"
                     /translation="LQSANAQSGTPAASPAADGEWSFTDDKNVTVTLPQRPVRVVADI
                     NTAAALWDFGVRPVAVFGWNVAADGSFNAAAGNVDPKAVEIVGNATVTIEPEKVAAVQ
                     PDLIVTLTFGPDNPDDYWSFMDHNVIEQVKQIAPIVAISGILRADEAVKRFGDLAAAL
                     GIDLESSTVAQDQAAFTAASNDFTAAVKGKSGLTAMFVAPTTEEFYIANPKIAGDIQF
                     FRNLGLEIPDVDTVNPTDYWETLSWEQSLKYETDVIFVSSRDPNAQDLLTSNAAFSKQ
                     PAVKAKQLGPWNQDFVLSYPGMTTILNGLLDTIKPAKADVVS"
BASE COUNT          351 a          664 c          645 g          402 t
ORIGIN      
        1 ggctgtcggc gttgggagtc ccgtcccgtt cgccataggt caggatgatg tcggcctcga
       61 cgagttggtc cagccgttcg taggagatca gctcgctcgt ccccgtcttg cccgtgaccg
      121 gatcgatcca ggttacgccg ctgtcggaga gcacgacacc cggtgcggaa tcggggaagt
      181 agagcgtgta ctggttatcc gccgagcccc atccgttggc aactgcccac gtgagggatg
      241 cgagttgatc ggcataggtc gtcttcatcg tgttcgtggc atcggcgtac gtcttcttca
      301 cgacgtcgag ttcgtcggtt cggccgatcg ccccggtgaa ttcgtccgca agcacctgcc
      361 aggcgttcgg cacggcgtaa tcgaagacca ccgtcggcgc gatttccttc agttgatcgt
      421 agacggatgt ggcccatggc gcattgattg ccaggatcac ctcgggattg agcgtcttga
      481 tcttttcgac gtcgatgccc tggtagctgc caaccgtcgg cacctttacc agtgcatcct
      541 tgtagaccga gatgtaggaa tcgaggtcga tcgccggaat cccgatcggg gtcaggccgg
      601 cgtcgatcag ggcggtgatc gaataggagt cgatgcagac gacccgggtc ggattcgccg
      661 gaacctcgac cggcccattg acggtatcga cggtgcgggt gccgccgacg ggtgacgcga
      721 ccggcgtctg ggcgcgcgcc gcggccggga gtccggccag cgccgcgatc gtcgcggcgg
      781 tcccgacgaa gcgccggcgc gtcaacgagg cgacgaggtg gcgaatctca tcctcgctcc
      841 acttctcgaa ggcgatctga aatgcggtgc ggttggctgt cgtcatggtc acgtgctctt
      901 gtttcctttt cctgtccaac tcgtcagggg cgggcaactg ccgaccccga attacccggt
      961 gcaacacttc tccattcgtc atcctgaacg aagtgaagga ccccccggcg aagccgatcc
     1021 gccgcaggcg gacacaaaca ccaacgctga tctgctcatc gcgccggcgg gcaacgacag
     1081 gccccgccac cacgggccac tcccctacga gacgacatcc gccttcgcgg gcttgatcgt
     1141 gtccagcagc ccgttcagga tcgtcgtcat tcccgggtag ctcagcacga agtcctggtt
     1201 ccacgggcca agctgcttcg ccttcaccgc cggctgcttg ctgaacgccg cgttcgacgt
     1261 cagcaggtcc tgcgcattgg gatcgcgcga actcacgaag atgacatccg tctcgtactt
     1321 gagcgactgc tcccagctca gcgtctccca gtagtccgtc ggattcacgg tgtcgacatc
     1381 gggaatctcg agaccgaggt tgcggaagaa ctggatgtcc cccgcgatct tcggattcgc
     1441 gatgtagaac tcctccgtgg tcggggcgac gaacattgcc gtcagaccgg actttccctt
     1501 gaccgccgcc gtgaagtcgt tcgaggctgc cgtgaaggcc gcctggtcct gcgcgaccgt
     1561 cgacgactca agatcgatcc cgagcgcggc ggcgaggtca ccgaagcgct tcaccgcctc
     1621 atcggcccgg aggatgccgg aaatcgcgac aatcggtgcg atctgcttca cctgctcgat
     1681 cacgttgtga tccatgaagc tccagtaatc atccggattg tccgggccga aggtaagcgt
     1741 cacgatcagg tcgggctgaa cggcggcgac tttctccggc tcgatcgtca ccgtcgcatt
     1801 gcccacgatc tcgaccgcct tcggatcgac gttgcctgcc gctgcattga acgagccatc
     1861 cgcggcgaca ttccacccga agacggcgac cggtcggacg ccgaagtccc acaacgctgc
     1921 cgccgtattg atgtcggcga cgacccgcac gggtcgctgc ggcagggtga ccgtgacgtt
     1981 cttgtcatcc gtgaaggacc actcgccatc ggcggcgggg gatgcggccg gtgttccact
     2041 ctgcgcgttc gcggactgaa gg
//
LOCUS       DASTMD010000269         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_916907, whole genome shotgun sequence.
ACCESSION   DASTMD010000269 DASTMD010000000
VERSION     DASTMD010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916907"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VFQ54_04050"
     CDS             complement(<1..792)
                     /locus_tag="VFQ54_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164192.1"
                     /translation="MTIDLSGEPESIDPAIAYAPRDWSIVHSIYDSLVQFGPNGEIVP
                     LAAETFTFSSPTTIDITLRQGVKFHDGSPLDTKAITRTVQHLNDSNSMVKDLFTVITK
                     VDEIDDLHAQITLSSPAAWLPAQMVAWFALLPDGFTPDSLVKAPIGTGPYTFREYKVG
                     SEITLDRNDDYTWGSPKGNSISDSAVYRFVSEPATRVADLATGSADIIIEIPEDQKSA
                     IESNGATAVEASLVGSAWIRLISDQKPFDDARVRQALNYAVDVQSI"
     gene            complement(1011..2555)
                     /locus_tag="VFQ54_04055"
     CDS             complement(1011..2555)
                     /locus_tag="VFQ54_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164193.1"
                     /translation="MKESSALSLSRRGLVKTAGASALGAASLFKFGLPGLAFAQTPVA
                     TPISELVIDLDGAPDNLDPALTYSVYDWSIAHSIYDALLHFDTDGTIVPLAAESFTTR
                     DAMTFDVKLRAGLTFHDGSPVTTAAITRAVDHLKAAKSQVTDLFTGITKVNEIDDLTA
                     QIVTETPSPWLPSQIAVWLMLFPEGMSDKTWTTAPVGSGPYKFDSYDAGNQIVLVRNE
                     AYTWDSPKGKPIADKVTYRFVTESATRVADLSTKTAEIIDGVPTDQRDAVKSAGGTAL
                     VEPILGTAFIRIATDTKPFDDARVRQAVNYAVDLQTIATQLVAPESKRIASLFPDPRG
                     LGFDPNLKPFAFDQDKAKSLLAEAGYPDGFKTTLEMVAGDSTDFAEAIVAQLADVGIE
                     VEIKQTELAAFNADWPTKTAPPLRYATWRPMYDPHTLLSLVFVSTGYLSRYQNPKADD
                     LIIGAAKEADPEKRKALYQQLGTLLQEEPAAIYLWNVVSTYGVAPEADSWQPRGDDYV
                     IATSRS"
BASE COUNT          520 a          831 c          911 g          522 t
ORIGIN      
        1 gatcgactgg acgtcaacgg cgtagtttag cgcctggcgg acgcgggcgt catcgaacgg
       61 cttctggtcg ctgatcagtc gaatccaggc ggatcccacc agcgacgcct cgaccgccgt
      121 tgccccgttc gactcgatcg ctgatttctg gtcttccggg atctcgatga tgatgtccgc
      181 agaaccagtc gcaaggtcgg ccacgcgggt cgcgggctcg ctcacgaacc ggtagaccgc
      241 gctatccgag atcgagttcc ccttcggact gccccaggtg tagtcatcgt tgcgatcgag
      301 cgtgatctcg ctcccgacct tgtactcgcg gaacgtgtat ggcccggtgc cgatcggggc
      361 cttgaccagc gaatcgggag tgaagccgtc gggaagcagt gcaaaccagg cgaccatctg
      421 ggccggcagc cacgccgcgg gactggaaag cgtgatctgc gcgtgcaggt cgtcgatctc
      481 atcgaccttc gtgatgacgg tgaagagatc cttcaccatt gagttggagt cattgaggtg
      541 ctggacggtg cgcgtgatcg ccttcgtatc cagcggcgag ccgtcgtgga acttgacgcc
      601 ttggcggagc gtgatatcga tcgtcgttgg tgaggaaaag gtgaacgtct cggcggcgag
      661 gggaacgatc tccccgttcg ggccgaactg gacgagtgag tcgtagatcg aatgcacgat
      721 cgaccagtca cgcggcgcat acgcaatcgc cgggtcgata ctttccggtt ctcccgatag
      781 atcgattgtc acagtagctg gcaatacatc ggattcaggg gaacccaccg gtgtcgattg
      841 agcggcaaca gaacgaagca tggaaattgg atcgagcaga ccaatcgaga gcgcgagcgc
      901 cccgctaccg cggaccagcc cgcgccggga aaggcgcgaa tgcccgaaat gagccgtccg
      961 actagaacaa tcagtggact tggaactcac gggactcctc caggaatcaa ctaggaacgg
     1021 gatgtcgcga tcacgtagtc gtcaccgcgc ggctgccagg aatcggcctc cggtgcgaca
     1081 ccatacgtcg atacgacatt ccataggtaa atagcggccg gttcctcctg caggagggta
     1141 ccgagctgct ggtagagcgc cttccgcttc tcgggatccg cttccttcgc cgcgccaatg
     1201 atgaggtcat cggccttcgg gttctggtag cgggagagat agccggtgct gacgaagacg
     1261 agactgagca gcgtgtgcgg gtcgtacatc ggacgccacg tcgcatagcg gagcggcggc
     1321 gccgtcttcg tcggccagtc ggcgttgaag gcggcaagct cggtctgctt gatctcgacc
     1381 tcgatcccga catccgccag ctgggcgacg atcgcctcgg cgaagtcggt gctgtcaccg
     1441 gcgaccatct cgagcgtcgt cttgaatccg tcgggatagc cggcttcggc caggagcgac
     1501 ttcgccttgt cctggtcaaa tgcgaacggc ttcaggttcg gatcgaatcc gaggccgcgg
     1561 ggatccggga agaggctcgc gatccgcttc gattccggcg cgacgagctg ggtcgcgatg
     1621 gtctgcaggt cgacggcgta attcaccgcc tggcggacgc gggcgtcgtc gaacggcttc
     1681 gtatcggtcg caatccggat gaaggcggtg ccgagaatcg gctcgacgag agccgttccg
     1741 ccagcactct tcacggcgtc gcgctggtcg gtcggaaccc catcgatgat ctcggcggtc
     1801 ttcgtcgaaa ggtcggcgac gcgggtcgcc gattcggtga cgaagcgata cgtgaccttg
     1861 tcggcaatcg gcttcccctt gggactgtcc caggtgtatg cctcattgcg aacaagcacg
     1921 atctggttgc cggcatcgta gctgtcgaac ttgtacgggc cggagccgac cggggctgtg
     1981 gtccacgtct tgtcgctcat cccttccggg aagagcatca accagaccgc gatctgcgaa
     2041 ggcagccagg gtgacggcgt ttccgtgacg atctgtgcgg tgagatcgtc gatctcgttc
     2101 accttcgtga tcccggtgaa gagatcggtc acctgcgact tcgcggcctt gaggtgatcg
     2161 acggcgcggg tgatcgccgc ggtggtgacc gggctcccgt cgtggaaggt cagaccggcc
     2221 cgcagcttga cgtcgaacgt catcgcgtcc ctggtcgtga acgactccgc ggcgagcggc
     2281 acgatcgtgc catctgtgtc gaagtgcagc agcgcatcat agatcgagtg cgcgatcgac
     2341 cagtcgtaga cggaatacgt cagcgccgga tcgaggttgt ctggcgcacc gtcgagatcg
     2401 atcaccagct cgctgatcgg ggtagccacc ggcgtctgcg cgaaggcgag accaggcagc
     2461 ccgaacttga agagcgacgc cgcgccgagc gcggaagcgc ctgccgtctt gacgagtccc
     2521 cgacggctca gggaaagagc tgacgattct ttcaacgcac gttctccaac aggtagtcca
     2581 gacgtacgac gcacgagcgg tcctccttta tgtccagcgc cgaaatggat cacttcgctc
     2641 ttgcagccaa tccgcaagca ggttgaatcc aacgactgcg agggcgatgg caatgccggg
     2701 gaagacgctt gcccaccagc cgttgaagat ctgttcttgc ccggcctgaa ccatcccgcc
     2761 gagcgtgatc cgctggacgg ggag
//
LOCUS       DASTMD010000270         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1988473, whole genome shotgun sequence.
ACCESSION   DASTMD010000270 DASTMD010000000
VERSION     DASTMD010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988473"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..1333)
                     /locus_tag="VFQ54_04060"
     CDS             complement(278..1333)
                     /locus_tag="VFQ54_04060"
                     /inference="COORDINATES: protein motif:HMM:NF021832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine salvage family protein"
                     /protein_id="HEU0164194.1"
                     /translation="MTTPSPLERSDPGSPFRDPLGILASTAYVIEKTTAVRIDREAVT
                     RLAAEMAVARTAPPPWDAALHFRDPAPDGGARTAGWIFALDALNFCFWSEEPDPDDRW
                     CVAWRGETHDGYNALAAALSRAVEEGRPLWDARWLAGVSAEELQDVLRPVPGSPGIPL
                     FAAWLANLHELGAGLLAMESTGESPVATLIARASGSAPALVQDVVRRFPSFNDVAIRD
                     GREIRFYKRAQILVADLAGALEGDPLGSFHDLASLTAFADYKVPQVLQRLGILVYEPE
                     LLARIRRRDLIPAGSDDEIAIRAATIQACEAIRLALQERSSIPFMASEVDWLLWNAGQ
                     SLPVGSDPYHRTLTVFY"
     gene            1569..>2772
                     /locus_tag="VFQ54_04065"
     CDS             1569..>2772
                     /locus_tag="VFQ54_04065"
                     /inference="COORDINATES: protein motif:HMM:NF021997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEU0164195.1"
                     /translation="MAWKPTFSETISRRNILKGAAAGGAGLALGASALSAGSAFAQAT
                     PSASPGTAGAVFDATSGEPQESYPLTTDAASFKVTVAASASVDDFSTNEFSKWFEERT
                     GVHIDWEVVPAANDAERNTALNVRMSGGDYGDMIMNFVPQPTVLQLYGQQGVFQPLND
                     LIDSSGVFTKRAYELYPLGRTAMTANNGKIYGLGQINDCYHCSMAQKLWINKVWLDNL
                     NLKMPTTTDEYAEVLREFKKGDPNKNGKADEYPLSGSPLAWHGFLDEYFMNSFIFHPG
                     AGTANKLRLIVVDGKVTPIYTQDAWKEGVKYLAGLFKEGLIDPESFTRDSDQLRQLGD
                     GNGGPDVVLGSVPAGWWGEFTTYNPQQQNAPWEQFTSVPPLKGPDGTQIAGFNPYAAF
                     SNATFMITD"
BASE COUNT          532 a          912 c          852 g          476 t
ORIGIN      
        1 gatgccctcg atcgggcgcg acgtggattg tcacaactgc gattttcaga tacccacctc
       61 ccggaccggg tgtggatgac ctgtccccag cttctccatc ccgattcgcc gcgccagatc
      121 aacgagatcg ggagttatcc ccgttatccc catcccctac tacttcgtct gtttctctct
      181 cttcataagt aaatactagg aacgaacacc gaccacgtta ggcacatcac ccacacgctg
      241 tcacacccgt gatgaccagg accgccctgc actcttccta gtagaagacc gtcaacgtgc
      301 ggtgatacgg atcgctgccg accggcagcg actgcccggc gttccacagc agccaatcga
      361 cctcgcttgc catgaacggg atcgacgacc gctcctgcaa tgccaggcga atcgcctcgc
      421 aggcctggat cgtcgcggcg cggatcgcga tctcgtcgtc gctgccagcg ggaatcaggt
      481 cgcgccgccg gatccgcgcg agaagctccg gttcgtagac gaggatcccg agccgctgca
      541 gcacctgagg caccttgtag tcggcgaagg cggtgagcga ggcaagatcg tggaaggaac
      601 cgagcggatc gccctccagg gcgccggcga gatctgcgac gaggatctgg gcacgcttgt
      661 agaagcggat ctcccgccca tcccggatcg cgacatcgtt gaaggaggga aagcggcgga
      721 cgacgtcctg gacgagcgcc ggcgccgatc cgctcgcccg cgcgatcagc gtggcaaccg
      781 gtgattcgcc cgtcgattcc atcgcgagca gcccggcgcc gagttcgtga aggttcgcga
      841 gccaggccgc gaagagcggg atcccgggtg agcccggaac cggtcgcagg acgtcctgga
      901 gttcctcggc gctgacaccc gccaaccagc gtgcgtccca gagcggcctc ccttcctcga
      961 cagcgcgcga aagcgccgcg gcgagtgcgt tgtagccatc atgggtttct cctctccagg
     1021 cgacgcacca gcgatcgtcg ggatccggct cctcgctcca gaagcagaag ttcagggcat
     1081 cgagcgcgaa gatccagccc gccgttcgcg ccccaccatc cggggcggga tcgcggaaat
     1141 gcagcgccgc atcccacggc ggtggcgcgg tgcgggccac cgccatctcc gcggcgaggc
     1201 gcgttaccgc ttcgcggtcg atccgcaccg cggtggtctt ttcgataacg tatgcggtcg
     1261 atgcgaggat gccgagcgga tcacgaaaag gtgagcccgg gtcagaacgt tcgagcgggg
     1321 atggcgtcgt catctgggga gtcgatcctt cgggtggggc cggcggagca gaaccggcgg
     1381 aagtctggcg tcccggcgtc cgggaggtat tgacagcagt catctgcggg aattacgctc
     1441 tctccagaaa tctcagggaa atgtatacag tatccggttc gcggcaattg cgcgtgccgt
     1501 cgacggatgc cgcggggcct gttcgatctg cgctcgaggt tcgtgttctc atcgaaggag
     1561 aagagaaagt ggcctggaaa ccaacgtttt ccgaaacgat cagccgccga aacatcctga
     1621 agggagccgc cgccggcggt gcgggtctcg cccttggcgc ctccgcactc tccgctggat
     1681 ccgccttcgc acaggccacc ccatccgcgt ccccgggaac tgccggtgcc gtcttcgatg
     1741 cgacatcggg cgagccgcag gagagctacc cgctcacgac cgacgcggca tcgttcaagg
     1801 taaccgtcgc cgcctccgcc tcggtcgatg atttctcgac caacgagttc tcgaagtggt
     1861 tcgaagagcg caccggtgtc cacatcgact gggaagtcgt cccggctgcg aacgacgccg
     1921 agcgcaacac cgccctcaat gtccgcatgt ccggtggtga ttatggcgac atgatcatga
     1981 acttcgtccc gcagccgacc gtgctccagc tctacgggca gcaaggcgtg ttccagcccc
     2041 tgaacgatct gatcgacagc tcgggagtct tcaccaaacg ggcctacgag ctctacccgc
     2101 tcgggcgcac ggcgatgacg gcgaacaatg gcaagatcta cggtctcggc cagatcaacg
     2161 actgctatca ctgctcgatg gcccagaagc tgtggatcaa caaggtttgg ctcgacaatc
     2221 tcaacctcaa gatgccgacc acgaccgacg aatacgccga agtccttcgc gagttcaaga
     2281 agggcgatcc gaacaagaac ggcaaggccg acgagtatcc gctttccggt tcgccgctcg
     2341 cctggcacgg gtttctcgac gaatacttca tgaacagctt catcttccat ccgggcgccg
     2401 gtacggccaa caagctccgc ctgatcgtgg tcgatggcaa ggtgacgccg atctacacgc
     2461 aggacgcgtg gaaggaaggc gtcaagtacc tcgccggtct cttcaaggaa ggattgatcg
     2521 atcccgagtc cttcacgcgt gactccgatc agcttcgcca gctcggcgac ggcaacggtg
     2581 gcccggacgt cgtgctcgga tccgtcccgg ccggctggtg gggtgagttc acgacctaca
     2641 acccgcagca gcagaatgcg ccgtgggaac agttcacgtc cgtgccgccg ttgaaggggc
     2701 cggacggcac gcagatcgcc ggattcaacc cgtacgccgc cttctccaac gcgaccttca
     2761 tgatcaccga ca
//
LOCUS       DASTMD010000271         4675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1006873, whole genome shotgun sequence.
ACCESSION   DASTMD010000271 DASTMD010000000
VERSION     DASTMD010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006873"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VFQ54_04070"
     CDS             <1..1418
                     /locus_tag="VFQ54_04070"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU0164196.1"
                     /translation="PAAPSETPTLVASPVAGTPIAPLVPATPIIGTSPTGAPPTVESS
                     PTPPGTPIAATTVATGTLEPTPSPTATIAASPTTEPTTESTATPALTPTTEPTEPTAT
                     GTASPTTEPTATPPMTSSVGYISGTDGDGAFCRTDPSSDGTVITLLPEGTEITTTGDA
                     VDGWQAVTCDGSAGYVFAEFVSNDAPPTVTPTQTPAETPTVESTLEPTATTDVATSAP
                     GADLDDMTPTATIPGSPTESTDESTQPPTMTSTLEPTVAPTVEPSATPTLEPTLTPTV
                     EPTATPTLEPSPTPTVEPTLVPTAEPQLVTTDKVVNVSGDASVTSANPDTAASGEQNG
                     LLTIGGPDGAVTVLTFDVEGVGTGTVVNASLVLTGAGDAAGSGGQVLVAPGTSFDENS
                     VTGTTVVNTPLSQIGPTGQIAPGVETTIEVTGVVTGDGTISFVIEGTPDQMLALTSRE
                     SGAPAYLDLTIQDWVYPESNP"
     gene            1592..2059
                     /locus_tag="VFQ54_04075"
     CDS             1592..2059
                     /locus_tag="VFQ54_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0164197.1"
                     /translation="MFKRIIVPLDGSEVAEQGLGKALELSKSLNAPLFLLRIVDTASI
                     TQLASAGGGAGSDFGLISEVLDEEQTDSKTYLEATISRLKGDGYDVSGEIAQGPIART
                     IVDAAKPEDVIVIGSHGRTGVQRWFLGSVAEDVIRHANCPVLLIRATHDETKS"
     gene            2237..3571
                     /locus_tag="VFQ54_04080"
     CDS             2237..3571
                     /locus_tag="VFQ54_04080"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164198.1"
                     /translation="MGPFHRPHNEKEVAGANVIKTPHPGEDRGDRFPAFKYRNYRLFF
                     SGQLVSVIGTWMQSTSEAWLVVDLLNASALQLALVSVCQFGPVLFLGIPSGILADRFS
                     KRNILLFTQTVFGLLALTMTTLIVTHTVQLWHVYAVALIYGVNTSIDQPARQSFVSEM
                     VGREAVMNAVSLNSAVFNSGRVVGPAVAGGLLAIFGPAVCFGINAASYIAVLIGLSMM
                     NVKPNLQRVSGSALKGIREGIAYVRATPAILRTIVLIGFIGTFAVNFNVWIPLLAQDD
                     FKAGAGTYGLLMASMGVGSLSGALSLALFGGRRPNRKRMLAAAMGLGIAEIALAVVAS
                     IPLPVYIGMVSLALVGFLSSNAMATANSTVQTTASDQYRGRVMAVYMTVFSGTTPLGA
                     LAAGFIAEHFSAPASVACGGFVAFVAALTLAWTQRERIKLPGVQPSHRSTTN"
     gene            complement(3611..4072)
                     /locus_tag="VFQ54_04085"
     CDS             complement(3611..4072)
                     /locus_tag="VFQ54_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0164199.1"
                     /translation="MAIEQVSRGWDRATSPDATGVSAESTPSGRISSGFGDRQRIGAS
                     IRRRRNERGMPLGDVAQRIGISTSHLSRIERGLTIPSYKLLGRIADDFGIEPSDLLAE
                     ERATVVVDDMLEQILEQVGMGAEARRDLLRLGNDTRAELASAMMKLTEQNS"
BASE COUNT          873 a         1597 c         1390 g          815 t
ORIGIN      
        1 taccggctgc cccgtccgaa accccgactc tggtcgcgtc gccagtcgcc ggtaccccga
       61 ttgcgccgct ggttccagcg acaccgatca tcggcacgag tccaacaggt gcgccgccaa
      121 cggtcgagtc atcaccgacg ccgccgggaa ccccgattgc ggcaacgacg gtcgcgaccg
      181 ggacactcga gccgaccccg tcgcccaccg cgacgatcgc ggcctcccca acgaccgagc
      241 cgacgaccga gtcaaccgcg acgcctgccc tcaccccgac gacggagccg acggagccga
      301 cggcaacggg caccgcgtct ccgacgaccg agccaaccgc cacgccgccg atgacgagca
      361 gcgtgggcta catctcgggg acagacggcg atggcgcctt ctgccgcacc gatccgtcga
      421 gtgatgggac ggtgatcacc ctcctgccgg aaggaaccga gatcactacg acgggtgatg
      481 cagtcgacgg ctggcaagcc gtgacatgtg acggaagtgc cggctatgtc ttcgccgagt
      541 tcgtgagcaa cgacgctccg ccgaccgtga cccccacgca aactcctgcc gagacgccca
      601 ccgtggaatc cacgctcgag ccaacggcca cgacggacgt cgccacgagc gcacccggag
      661 ccgatctcga cgacatgacc ccgaccgcaa cgatcccggg gtcgccgacg gaatcgaccg
      721 atgaatccac gcaaccgccg acgatgacgt caacgctcga gccaacggta gccccgacag
      781 tcgagccatc cgcgacgcca acccttgaac caacactgac cccgacagtc gagccgaccg
      841 cgacgccaac cctcgaacca tcacccacgc cgacggtaga gcccaccctc gtcccgaccg
      901 cggaaccgca gctcgtgacc accgacaagg tcgtcaacgt gagcggcgac gccagtgtca
      961 cgtccgcgaa tcccgatacg gcggcatcag gagagcagaa cgggttgctc acgatcggcg
     1021 gaccggatgg cgccgtgacg gtcctcacct tcgatgtcga gggagtcggg accggaaccg
     1081 tcgtcaacgc cagtctcgtt ctgacgggcg cgggtgacgc agcgggctcc ggcggccagg
     1141 tgctcgtcgc gccgggaacg agcttcgatg agaactccgt gaccggtacg actgtcgtca
     1201 acacgccgct gagccagatt ggcccgacag gccagatcgc tcccggcgtc gaaacgacga
     1261 tcgaggtcac gggcgtcgtc accggcgacg gaacgatctc gttcgtgatc gagggcactc
     1321 ccgatcagat gctcgccctg accagccggg agagcggtgc ccccgcctac ctggatctga
     1381 cgattcagga ctgggtgtat cccgagtcca atccgtagcg aggacgtttc ccggtccggc
     1441 atgaacggcg gaacgggtgt cgggctccga cacccgttcc gcgatcggca tcttgtcgat
     1501 tggcccacga tctgcgatat cacttctgca tgaagatgtc gcgaggcggc gccttccgcg
     1561 cgcaacgccc gttcaaagca gggagcttcc catgttcaaa cgcatcatcg tccctctcga
     1621 cggctctgaa gtcgccgagc aggggctcgg caaggccctc gagttgagta agtcgctcaa
     1681 cgcccccctc ttccttctcc gcatcgtcga taccgcctcg atcacccagc tcgcgagcgc
     1741 cgggggcggc gctggaagtg acttcgggct gatctccgaa gtcctcgacg aggagcagac
     1801 ggattccaag acctatctcg aggcaacgat cagccggctg aagggcgacg gatacgacgt
     1861 ctccggggag attgcccagg gccccatcgc caggaccatc gtcgacgccg ccaaacccga
     1921 ggatgtcatc gtcatcggct cccacggtcg caccggcgtg cagcgctggt tcctgggaag
     1981 cgtcgcggag gacgtcatcc ggcacgcgaa ctgcccggtg cttctgattc gtgccacaca
     2041 cgacgagacg aagagctgat cgcggcaacg gactccgatt gtcaaatgat gtaaacaatc
     2101 acatgtcaac atccgttgac acgatctgaa ttctgtgcga tctctgggga gcgagaaacg
     2161 acgaggaagt caggcagcag ccgcttccgg atcgccactc ctcccctcgg tcgttcgcgg
     2221 cgtcacagga aaattcatgg gcccctttca ccgtcctcac aacgaaaagg aggtagctgg
     2281 tgcgaatgtc atcaagactc cgcaccccgg tgaggatcgt ggagacaggt ttccggcatt
     2341 caagtaccgg aactacaggc tgttcttctc tggccaactc gtctcggtga tcggtacgtg
     2401 gatgcaatcc acgagcgagg catggctcgt cgtcgatctc ctcaacgcat cggccctcca
     2461 gctcgcactg gtgagcgtct gccagttcgg ccccgtcctt ttcctcggca ttccgtccgg
     2521 aatcctggcc gaccgcttct cgaagcgcaa tatcctcctc ttcacgcaga ccgtcttcgg
     2581 cctgctggcg ttgacgatga cgaccctgat cgtcacgcac acggtccagc tctggcacgt
     2641 ctatgccgtc gcgctgatct acggggtcaa tacctcgatc gatcagccgg cacgacagtc
     2701 gttcgtctcg gagatggtgg gcagggaggc agtcatgaac gccgtctccc tcaactctgc
     2761 cgtcttcaat tcgggacgcg tcgttggtcc ggcggtcgcc ggagggctgc tggcgatctt
     2821 cggtccggcg gtctgcttcg ggatcaacgc ggccagctat atcgccgtgt tgatcggtct
     2881 ctccatgatg aacgtcaagc caaacctgca gcgcgtgagc ggatccgcac tcaagggcat
     2941 ccgcgagggc atcgcctatg tgcgggcaac cccggcgatc ctgcggacga tcgtgctcat
     3001 cggcttcatc gggacgttcg cagtcaactt caacgtctgg attccgctgc tcgcgcagga
     3061 cgacttcaag gccggcgccg gcacgtacgg attgctgatg gcgtcgatgg gtgtcggctc
     3121 gctctcgggg gcactctcgc tcgcgctctt cggcggtcgc cgtccgaatc gcaagcgaat
     3181 gcttgccgcc gcgatggggc tgggcatcgc cgagatcgcc ctcgccgtcg tcgcctccat
     3241 cccgctcccc gtctacatcg gcatggtctc actggcgctg gttggattcc tgagcagcaa
     3301 cgcgatggcg acggcgaact cgaccgtaca gacgacggcg agcgaccagt atcggggccg
     3361 cgtgatggcg gtctacatga ccgtcttctc gggcaccact ccgctcggcg ccctcgccgc
     3421 cggattcatc gccgagcatt tcagcgcccc ggcgtcggtt gcctgcggtg gtttcgtcgc
     3481 cttcgtcgcg gcgctcacgc tcgcctggac gcagcgtgag cggatcaagc taccgggcgt
     3541 ccaaccctcg caccgatcga ctacgaactg aatcctcgcc gttccgggtg aacgacgcgc
     3601 ccggccaggt tcaggagttc tgctccgtca gcttcatcat cgcgcttgcg agttcggcgc
     3661 gagtatcgtt gccgaggcga agcaggtcgc ggcgcgcttc cgcgcccatg ccaacctgct
     3721 ccaggatctg ctcgagcata tcgtcgacca cgaccgtggc gcgctcttcg gcgaggagat
     3781 cggacggttc gatcccgaaa tcatcggcga tgcgaccgag aagcttgtat gaagggattg
     3841 tcagaccacg ttcgatccgg ctgagatgag atgtcgagat accgatgcgc tgggcgacat
     3901 cgcccagtgg catgccacgt tcgttgcgac ggcgacggat cgaggcgccg atgcgctgcc
     3961 gatcgccgaa ccccgaactg atccgccccg atggcgttga ctctgcagag accccggtgg
     4021 cgtcaggaga cgtagcgcga tcccaacccc gggaaacctg ttcgatggcc acgacatcgt
     4081 ctcctgtcct gaatccgtta tcgccccacc tcatcccggc gagcgaaacg gcttgtccca
     4141 caggccgggc agcccgccca gcatgagaac aacgtatggc tgatccaggc gacacctctc
     4201 ggacggcaaa tggatcattg ccacgcatta ttcttgttga atagtacact cgctgcctca
     4261 tgcaaccttg gaccgggatc aattcttcct gtcaggcgac gttcatagcc aacaccctgt
     4321 cgtcctggct gcctgaatcc catcccgaac ttatgtcata cgctcacaag aaatcccggg
     4381 gattccgccg tggcccgtcc ctgggtcaat gcccgaacgc atcaagggta caattgcctg
     4441 caggttccac gatagctccc catgcgccac tgacaggcgt ggatagaatc ggcgagcccc
     4501 ggcccgacgt tgaccaagga cgcatgcgaa atcgatgggc tttgacccct tcctcctcat
     4561 cagcctcttc tacgtcgtca tcggcgtgcg cgtcgtcatc cagctcgtgc agagatggtc
     4621 gagcgtatgg gacctgaact tcacgcaaca ggatcggatg ctggtcgacc aggct
//
LOCUS       DASTMD010000272         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_292787, whole genome shotgun sequence.
ACCESSION   DASTMD010000272 DASTMD010000000
VERSION     DASTMD010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292787"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1012
                     /locus_tag="VFQ54_04090"
     CDS             77..1012
                     /locus_tag="VFQ54_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164200.1"
                     /translation="MTESPANRTSHPIVLTSAQDPSFPAAETLRQLVESGSGNGKQAT
                     LLRVALLRRDYPDARIEIDLAHHDPQATIIKASVTLPTGASASGFATGSVSRLHLIET
                     EAIANALDLLGHTLIVAGATEPASFEPATHPASDEVSAGVPAYDNTPFDPDVAPEELE
                     DATAETAPFSPVRSDMESVPPERQVLPRPEAPRIGQPTPRAPGTPPAVIDAVRRSNLR
                     RHTPMSIPDRPDDGRRVTAPTTPAPRDDAPPVAADANASDQPPLEDYSWTAFWRVARP
                     QGLDKVKVEALIGRTIEGMNPGDVRTALAAAGVEI"
     gene            1889..>2836
                     /locus_tag="VFQ54_04095"
     CDS             1889..>2836
                     /locus_tag="VFQ54_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164201.1"
                     /translation="MTAVIKKVASTDSTSSSPDNPKIYTHRQGCTRFGRRRVVSIVGM
                     IAIAAIVLATFHQSISSAQEEGSTPETSPVASPAATPDAGAQKLTVTFTEYNDSGIKG
                     TATFYDEGARTLVVIDVKNAGTDHPAHIHAGTCNTLDPKAAYNLIDIHEDGQSSTYID
                     TSLSDLTASQFAIDLHLSPNDLGTMIACANISGTITNAQGTPVAPTPPATVTEETPTP
                     ETAVGGDGTSGSNMPTPTQIAGMGDNTTTEITPTSEAVTGETPTEPAEAVTTDETPTQ
                     APAAVIIIGNETPTTEANGGDGAGAGAVWTSTNTSSALGT"
BASE COUNT          571 a          984 c          836 g          445 t
ORIGIN      
        1 ctatgaacat cagttcgcat gcctcgcgca gccgcccgtt tctcgccggt gtcagcccct
       61 atcggagtcg ccagccgtga ccgaatcacc cgccaaccga acgagccacc cgatcgtcct
      121 cacgtcggcc caggaccctt cctttccggc agcggagacc ttgcgccaac tcgtcgagtc
      181 cggttccggc aacggaaagc aggcgaccct gctgcgcgtg gcgctccttc gtcgcgacta
      241 cccggacgcc cggatcgaga tcgacctcgc gcatcacgat ccccaggcga cgatcatcaa
      301 ggcatcggtg acactgccga ccggcgcttc ggcgagcgga ttcgccactg gttccgtgtc
      361 ccgcctccat ctgatcgaga cggaggcgat agccaacgcg ctcgatctcc tcggtcacac
      421 gctgatcgtc gccggcgcca cagagccggc gtcgttcgaa ccggcaacgc acccggcatc
      481 cgatgaagtg agcgccggcg tgccggcata cgacaacacc ccctttgacc cggatgtggc
      541 gcccgaagag ctcgaggacg cgaccgccga aacagcaccc ttctccccgg ttcgttccga
      601 tatggagtcg gtcccaccgg agcgacaggt gctgccgcga ccggaggcgc cccgcatcgg
      661 ccagccgacg ccacgcgctc ccggtacccc gccagcggtg atcgacgccg tccgccgttc
      721 gaacctccgg cgccacacgc cgatgtccat ccccgatcgg ccggatgatg gacgtcgcgt
      781 gaccgcgccc acgacgcccg ccccgcggga cgacgccccg ccagtggctg cggacgccaa
      841 tgcgagcgac caacctcccc tcgaagacta ttcatggacg gcattctggc gcgtcgcgcg
      901 tccgcagggc ctcgacaagg tgaaggtgga agcgctgatc gggaggacga tcgaggggat
      961 gaaccccggc gatgtccgca ccgctctcgc cgccgccggt gtcgaaatct agcgttctcg
     1021 tcgcggcgaa ggatgaacgt ccgcccgaca aacccctgtg gttccgacaa cgtttcggtc
     1081 atggcgccca aagccttcac gacctcgcct tcgcgatccc gtaagggcga acctgatgtt
     1141 cgccctgatc gtcggaaacc ggtgtcctgc agccccccat gacacatcat cccgagcgga
     1201 gcgcagccgc ggcgccgtca ggcgcgcagt gaagcgcagt cgagggacca cgttccattc
     1261 gcgctgtctc gctgcctccc cccccgacga gcgcgccgct ggggaaacac gcggatgcga
     1321 accgcacccc caccataacg gaggacgcac ccgatcaatc ccgggacgcg gcgcgtcgca
     1381 gtcgtgcccg gagtcaacgt gtgtccgtgt ggctccggca acacatatac ggacactgac
     1441 atcattccga ctgatatcca tgtccgtacg ggctattcag gaaactgagg ttgatcaatc
     1501 tgtacgttca gcctatcgcg atcatgatta ccacgtccgt acatgatcaa gataagtctt
     1561 ataccgacct taaagcatcc tgaggatatc ctgtaatcgt gctagacctg tcaaatcgtg
     1621 ccatattggc actgaacgta ctcttgtagc gattcgggtc gacatgtagt ctgggtctca
     1681 gtcacagggg gagggcactc gtgccgaaga cctcatgacg aaggtcgcgc cggtcaccca
     1741 cggggcaagg cgcgcctcca tccagatcct cacgcacctt ggcctgaacc acggttcagc
     1801 ggtttcgagg tctggaacgg ctgggccgat ggtcagggtg gctgtcggat gttttcgtca
     1861 ttacgacgcg gctggggaga gtgtcgacat gacggcggtc atcaagaagg tggcgtccac
     1921 ggactccaca tcgtcatcgc cagataatcc gaagatctat acgcaccggc agggttgcac
     1981 gcgattcggt cgccggcgcg tcgtgtcgat cgttggcatg atcgcgatcg cggcaatcgt
     2041 gctcgcaacc ttccatcaat cgatcagcag tgcgcaggaa gaaggctcga cgccggagac
     2101 ctcgcccgtc gcatcaccgg ctgcgacgcc ggatgccggc gcccagaagc tgaccgtgac
     2161 cttcaccgaa tacaacgact ccgggatcaa gggcacggca accttctacg atgaaggagc
     2221 acgcacgctc gtcgtcatcg acgtcaagaa cgcgggcacg gatcatcccg cccacattca
     2281 tgcaggcacc tgcaacaccc tcgatccgaa agcggcctat aacctgatcg acatccacga
     2341 ggatggccag agcagcacct acatcgatac ctcgttgagt gacctgacgg cctcccagtt
     2401 cgcgatcgat ctgcacctgt cgccgaacga cctcggcacg atgatcgcct gcgcgaacat
     2461 ctccggtacg atcacgaatg cccaggggac gccggtcgct ccgacaccgc ccgcgactgt
     2521 gaccgaggag acgccaacgc cggaaaccgc cgtcggcggt gatgggacga gcggctcgaa
     2581 catgccgaca ccaacccaga tcgccggaat gggtgacaac acgacgaccg agatcacgcc
     2641 gacttctgag gcggtgaccg gagaaacccc gaccgagccg gccgaggcgg tgacaacaga
     2701 cgagacaccg acccaggcac ccgccgcggt gatcatcatc gggaatgaaa cgccgacgac
     2761 ggaagcgaac ggcggcgacg gagccggcgc cggagccgtc tggacgagca ccaacacttc
     2821 gagcgcactc ggcacc
//
LOCUS       DASTMD010000273         3538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2435572, whole genome shotgun sequence.
ACCESSION   DASTMD010000273 DASTMD010000000
VERSION     DASTMD010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2435572"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VFQ54_04100"
     CDS             <1..624
                     /locus_tag="VFQ54_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU0164202.1"
                     /translation="RPQLEALALADSVALDPHKWLYVPIEAGLILVKDAEAMRRTFSL
                     VPDYLPSGAAPTGVNGLPWFSEYGSQQSRGFRALKVWMALRFHGLAGYRAAIDHDLDQ
                     AARLVSLIDDTPDLEIVAPPSLSIVCFRFTAPGADLDTASLNALNQAILEDVQLGGEA
                     FLSGTTIDGRFALRACIMNPRSSGGDIDALVRIIRNAASRHAAARLS"
     gene            1279..1950
                     /locus_tag="VFQ54_04105"
     CDS             1279..1950
                     /locus_tag="VFQ54_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU0164203.1"
                     /translation="MTDAETRTHRTATYSLDELTERSGVTARTVRYYIAEGLLPPPNN
                     AGPRATYSDTHLNRLHLIGRLKEAYLPLREIRRQLQALSDAEIDAAVATETPPADAVA
                     DPGGRQRLRSALGGMRFQQTDSRESAADYLKRVSSEPEQDDRPDHDRGHIRRRLPAPP
                     AMPRPGMPWIDPTSPTAPEAGTNWKRLSIAREAELLIEESTYERRRQQIDSAIEWMRR
                     ILNNS"
     gene            2016..3314
                     /locus_tag="VFQ54_04110"
     CDS             2016..3314
                     /locus_tag="VFQ54_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0164204.1"
                     /translation="MSTEVTPSTLQVTTAWERPLVGVDGAETALVVTIAATAGADPMQ
                     RTPVDVAFVLDHSGSMAGQKLDLVKEAVDVALDHLAAKDRVSLIVFDSEVGTLHKLRE
                     ADAGTKATIRRRLNRVEAGSSTYLSGGWFAGCQELAEHQSKDDPRPRRALLLTDGLAN
                     EGITEPHALTKHATELRLRGITTTAIGVGSGFDEMLLGGMAEAGGGTFQYIAQPSELS
                     AFFENEIGNLLAITAIRPTLRLTLPDGIHAHLVNAFPRHREGKTITIDLRDLSVGDEV
                     RLVFDTSVAPGAEGSSMAPTFELRWTDAISNQRSRIQGEAESIVRVAAEDAADVLVDD
                     DVAAIVALERAAKAQRKAVELDRAGRFEESRSAFAVAHNVMAAAPATPEIELERRLTQ
                     SLANAPLIALREEVRKERIFSNLRRSRGTRPDPRQGDRLD"
BASE COUNT          622 a         1258 c         1082 g          576 t
ORIGIN      
        1 cggccacaac tcgaggccct cgcgctggcg gacagcgtcg cgctggatcc gcacaagtgg
       61 ctctacgtgc cgatcgaggc cggactcatc ctcgtcaagg atgccgaggc aatgcgtcgc
      121 acgttcagtc tcgtccccga ctacctcccg tccggcgccg cgccaaccgg tgtcaacgga
      181 ctcccctggt tcagcgagta cggttcccaa cagagtcgtg gcttccgcgc actcaaggtc
      241 tggatggcgc ttcgcttcca cggtctcgcg ggataccgcg cggcgatcga ccacgatctc
      301 gatcaggccg cccgtctcgt ctcgctgatc gacgacactc cggatctcga gatcgtcgcg
      361 ccgccgagcc tgagcatcgt ctgcttccgg ttcacggctc cgggagcgga tctcgacacc
      421 gcgtcgttga acgcgctcaa ccaggcgatc ctcgaggatg tgcaactcgg cggcgaggcc
      481 ttcctctccg gcacgacgat cgatggccgc ttcgcgctgc gcgcctgcat catgaacccc
      541 cggagcagcg gcggggatat cgacgccctc gtccgcatca tccgcaacgc cgcatcgcga
      601 cacgccgccg cccgtctgtc gtaaagacgc gcttccattc ccgtccccac ggcgcttgtc
      661 gccgtttcgg attggcggag ccgatccgtc tccagaaacc gtccgttccc gctggggctc
      721 tcgtcgtccg ctcacggcga tgggatggga gcgatttcgg tatggcacgc gcacccacac
      781 gtcattccga gcgaagcgcc gacgcgacgc cgtcaggcgc gcgtccgtag gggcgatcct
      841 cgtgatcgcc cgggaccacg tcccactggc gacgcctcgc ctgtctgctc tccctccgac
      901 caacgcgccg tgccgggagc acgtggacgc cagctgatcc ctcgacaggc tcgggaggac
      961 gtgtcttgta tgtcgtcgcg cgggaagcac atgctcattc ccatccccac ggttcccggg
     1021 cggcggcgtg ccgggagcac gtggacgcga gctggtccct cgacaggctc gggaagacgc
     1081 ggctggggag gtgtcgtgcg gggagggtgc gacctgtgag atgttcgcga tatgcctgag
     1141 gtctccgggc gaagcatgtc agtgattgca tattgacagt agtacgtcac actgttacga
     1201 tatcgatcga agcttcagga atgtcccatc gatccacgac gacgtatccc tccggagcgt
     1261 tcagacaagg aggacgacat gacggatgcg gagacccgga cgcaccgaac cgccacctat
     1321 tcgctcgatg aactcaccga acgctcgggc gtcaccgccc gtaccgtgcg ctactacatc
     1381 gccgaaggtc tgctcccgcc gccaaacaac gccggaccgc gcgcgaccta ttccgacact
     1441 cacctcaacc gcctccatct catcggtcgc ctgaaggaag cctaccttcc gctccgggaa
     1501 atccggcggc agctgcaggc gctctcggat gcggagatcg acgcagcggt cgccaccgaa
     1561 acgccacccg cggacgccgt tgccgatccc ggcggtcgtc agcgcctccg ctccgcactc
     1621 ggcggaatgc gcttccagca aacggattcg cgggagagcg ccgccgacta cctcaagcgg
     1681 gtgtcttccg aaccggaaca ggacgatcgc ccggatcacg atcgcggcca catccggcgc
     1741 cggctgcccg ctccaccagc gatgcctcgc ccgggaatgc cctggatcga tcccacatca
     1801 ccgacggctc cggaagccgg cacgaactgg aagcgcctct cgatcgcgcg ggaagccgaa
     1861 ctcctgatcg aggaaagcac ctacgaacgg cgacgccagc agatcgactc cgcgatcgag
     1921 tggatgcgcc ggatcctcaa caactcctga tcgacctcgc tcccacccac aggacaaccc
     1981 tgtcatcccc cttatcagga aaggacgcca tcaccatgtc tactgaagtc accccatcaa
     2041 cgcttcaggt caccaccgcc tgggaacgcc cgctcgtcgg cgtcgatggc gccgagacgg
     2101 cactcgtcgt caccatcgcg gccaccgctg gcgcggatcc gatgcaacgc acgcccgtcg
     2161 atgtcgcgtt cgtgctcgat cactcgggat cgatggcggg ccagaagctc gacctcgtca
     2221 aggaggccgt cgacgtcgcg ctcgatcatc tcgcggcgaa ggatcgtgtt tccctgatcg
     2281 tcttcgacag cgaggtcggc acgctccaca agctccggga agcggatgcc ggaacgaagg
     2341 cgacgatccg ccgccgtctc aatcgcgtgg aggcgggctc ctcgacctat ctctccggag
     2401 gatggtttgc cgggtgccag gaactcgccg agcaccaatc gaaggatgat ccgcgtcccc
     2461 gccgggcgct tctgctcacc gatggtctcg ccaacgaggg catcaccgaa cctcacgccc
     2521 tgacgaaaca cgcgaccgag ctccggctcc gcggcatcac gacgaccgcg atcggcgtcg
     2581 gctccggctt cgacgagatg cttcttggag gaatggcgga agcgggcggt ggcacgttcc
     2641 agtacatcgc gcagccgtcc gaactgagcg ccttcttcga gaacgagatc gggaatctgc
     2701 tggcgatcac ggcgattcgc cccacgcttc ggctgacctt gcccgatggc attcacgcgc
     2761 atctcgtgaa tgcgttcccc cgtcaccgcg aggggaagac gatcacgatc gacctccgcg
     2821 acctctcggt tggcgatgag gtcaggctcg tcttcgacac cagcgtcgcc ccgggcgccg
     2881 agggatcgtc gatggcgccg acattcgagc ttcgctggac ggacgcgatc tcgaatcagc
     2941 ggtcgcgcat ccagggcgag gcggaatcca tcgtacgggt cgccgcggaa gatgcggcag
     3001 atgtgctggt ggatgacgat gtcgcggcaa tcgtcgccct cgaacgcgcc gccaaggccc
     3061 aacggaaggc ggtcgaactc gatcgcgccg gacggttcga ggaatcgcgc tcggccttcg
     3121 ccgtcgccca caatgtgatg gcggccgccc cggcgacgcc cgagatcgag ctggagcgga
     3181 ggctgacgca gtcactcgcc aacgcaccgc tgatcgcact cagggaagag gtccgcaagg
     3241 agcgcatctt ctcgaacctc cgccgctcac gcggaacacg ccccgacccg cgccagggcg
     3301 accgcctcga ttgatctcat cagaagaccg ccgggggatc cttcctccgg cggtcttcat
     3361 tgcgtcaggc gggccgctcg atctccggct cgtcatcgcg accggcgatc tcgttccaca
     3421 actcgtagtc cgtctcccgc ttggcgacca gccgaccgcg aggaccagcg acctgtccgc
     3481 cgagcgcctt gaacggccgg cgaccggatt catcgagatc gagatagacg gcgccttg
//
LOCUS       DASTMD010000274         1942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1274970, whole genome shotgun sequence.
ACCESSION   DASTMD010000274 DASTMD010000000
VERSION     DASTMD010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274970"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="VFQ54_04115"
     CDS             complement(<1..1556)
                     /locus_tag="VFQ54_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU0164205.1"
                     /translation="MSTSLIGDLGQVDAGHVVLDPITVEVISHAVSSIVEEMGETLVK
                     ASYSNNIKERRDCSTALFDAQGRTLAQAEHIPIHLGSLMGIVGEILLRYPLDAIQEGD
                     AFVGNDAYTGGGTHLPDIVIATPIFFAGEIVGWASNLAHHADFVDRGGQHIFQEGLRI
                     PPIRFQRDGEIIQDVLDLILLNCQVPDERRGDLRAQVAANRVGVVRFQGLCAKYGVEM
                     VRATGEALLDYAERQTRAGILRIPDGVYTFSDKLDATGLDEILDLHVSITVRGDEMWL
                     DFDGNPPQVRGGLNMVWTALLATVYYAAKTVIDPEILPNAGLYRPIHVSAPSGTILNC
                     VPPAGVNGRTTTCQRVVDLIHGAFAEAMPDRIIAACNGTNSGLSFHGIDPRTGKFYYY
                     MESMGGGFGARATKDGLDGVQVHITNTSNLPVECLEVEHPLLVERYELVNDSGGPGTW
                     RGGMGIHRRVRVLDHEAISGKGATREKTAPWGLFGGLEGGRMSVTSSTSDEPVGRQWK
                     LRAGDAISMRT"
     gene            complement(1587..>1942)
                     /locus_tag="VFQ54_04120"
     CDS             complement(1587..>1942)
                     /locus_tag="VFQ54_04120"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164206.1"
                     /translation="ERFDRAHDQFYGYAAPDDPVQFVTFRLEAIGLVRKAEMRPSPLA
                     GPDASAALTGERPVYFPEADGFVSTPLYDRSSLLPGNELVGPAIVDQLDATTVILPGQ
                     RAVVDLYRNLIITVD"
BASE COUNT          334 a          641 c          632 g          335 t
ORIGIN      
        1 gttcgcatcg agatcgcgtc gccggcccgg agtttccatt gacgtccgac cggttcatcg
       61 ctggtgctgc tggtgaccga catccggccg ccttcgaggc caccgaagag accccacggc
      121 gcggtcttct cgcgcgtcgc gcccttgccg ctgatcgcct cgtgatcgag gacgcgcacg
      181 cgacggtgaa tgcccatgcc acctcgccag gtccccggcc cacccgaatc gttgacgagc
      241 tcgtatcgct cgacgagcag cggatgctcg acttcgaggc attcgacggg caggttgctg
      301 gtgttggtga tgtggacctg gacgccgtcc aagccgtcct tcgtcgcccg ggcgccgaag
      361 ccgcctccca tcgattccat gtagtagtag aacttgcccg tgcggggatc gatgccgtgg
      421 aagctgagcc cggagttcgt cccgttgcag gcggcgatga tccggtcggg catcgcctcg
      481 gcgaaggcgc catggatcag gtcgacgacc cgctggcagg tcgtcgtccg cccgttcacc
      541 ccggccgggg gcacgcagtt gaggatcgtt ccactcggcg ccgagacgtg aatgggacgg
      601 tacagccccg cattcggcag gatctcggga tcgatgacgg tcttcgccgc gtaatagacc
      661 gttgccagca acgccgtcca gaccatgttg agaccaccac gcacctgcgg tggattgcca
      721 tcgaaatcga gccacatctc gtcgccgcgc accgtaatcg agacgtgcag atcgagaatc
      781 tcgtcgagac cggtcgcgtc gagcttgtcg gagaaggtat agacgccatc cgggatacgc
      841 aggattccgg cgcgcgtctg ccgctccgcg tagtcgagca gggcctcgcc cgtggcgcgg
      901 accatctcga cgccgtactt ggcgcagagt ccctggaagc ggacgacgcc gacccggttc
      961 gcggcaacct gggcacgcag gtcgccgcgg cgctcatccg gcacctggca attgagcagg
     1021 atcagatcga gcacgtcctg gatgatctcg ccatcccgct ggaatcggat cggagggatg
     1081 cgcaggccct cctggaagat gtgctgcccg ccgcgatcga cgaagtcggc atggtgcgcg
     1141 agattgctcg cccagccgac gatctcaccg gcgaagaaga tcggggtggc gatcacgatg
     1201 tcggggaggt gggtgccgcc accggtgtag gcgtcgttcc cgacgaaggc atccccttcc
     1261 tggatcgcgt ccaatgggta gcgaaggaga atctcgccga cgattcccat cagcgatccc
     1321 aggtggatcg ggatgtgctc ggcctgggcc agcgttctgc cctgggcgtc gaagagcgcc
     1381 gtcgagcaat cccggcgctc cttgatgttg ttggaatagg acgccttgac cagcgtctcc
     1441 cccatctcct ccacgatcga tgacaccgcg tggctgatca cctcgaccgt gatcggatcc
     1501 aacacgacat ggccggcatc gacctggccc aggtcaccga taaggctcgt actcatggag
     1561 cgatcctcgc tttccgatcc gccggctcag tcgacggtga tgatgagatt tcgatagaga
     1621 tcgacgaccg cacgctgacc cggcaggatc accgtcgtgg catcgagttg atcgacgatc
     1681 gccggcccca ccagctcgtt gccggggagc agggacgatc gatcgtagag cggcgtcgac
     1741 acgaagccgt cggcctcggg gaaatagacg gggcgctcgc ccgtgagcgc cgccgacgcg
     1801 tccggccccg ccaacgggct cggtcgcatc tccgccttcc gtacgaggcc gatcgcctcc
     1861 agccggaagg tgacgaactg cacgggatca tccggtgcgg cgtagccata gaactggtcg
     1921 tgcgcgcgat cgaaccgctc cc
//
LOCUS       DASTMD010000275         1712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_738415, whole genome shotgun sequence.
ACCESSION   DASTMD010000275 DASTMD010000000
VERSION     DASTMD010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738415"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VFQ54_04125"
     CDS             complement(<1..490)
                     /locus_tag="VFQ54_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU0164207.1"
                     /translation="MTPMVKTLAGLAGGWALGRIAATLSRLQAGHGTSAARKLHSRRL
                     FVRNATLGGAGVVLLEAGVGFFYMLWPNKTGAFGGLIAVPAASVPPVEGTPYRDTAGK
                     FYVVHTADGVEALYWKCVHLGCTVPWIESEKHFNCPCHGYVYLYDGTRIAGPAPRALD
                     AMP"
     gene            complement(600..1394)
                     /locus_tag="VFQ54_04130"
     CDS             complement(600..1394)
                     /locus_tag="VFQ54_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164208.1"
                     /translation="MAELAERPPAPQPGNAIDEQKRQKLLELVRGRALARPTDLADDE
                     VMVWPSLVVVEAVCAIVFLAILTVLSVLMNGPLLERANPNQTPNPSKAPWYFLNLQEL
                     LLHMDAGLAGVILPTVALAGVAAIPYFDRSPLGVGILGTSSKGRQIIAFSAVYTTVVI
                     SGMILLDEAIGRGTQFGLGRYTQEHWGAPEWVGGILISTLIVLIAIGVLVVLTKGIFR
                     PTTRELIIALFTGFYVSYIVLTVFGTSFRGVGQNLTLPWDLPLVHH"
     gene            complement(1464..>1712)
                     /locus_tag="VFQ54_04135"
     CDS             complement(1464..>1712)
                     /locus_tag="VFQ54_04135"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEU0164209.1"
                     /translation="GYLLPWDQLAFWAITVGSEIGGAAPIIGPQVNRILLGDYQIGQS
                     TLIRFYTLHVIFLPLIAAFLMAVHFWRIRKDGGISGPL"
BASE COUNT          327 a          512 c          600 g          273 t
ORIGIN      
        1 ccggcatcgc gtcgagcgca cgtggggctg gtccggcgat tcgcgttccg tcgtagagat
       61 agacgtaacc gtgacacggg cagttgaagt gcttctcgct ctcgatccac ggcaccgtgc
      121 agccaagatg cacgcatttc caatagagtg cctcgacccc gtccgcggtg tgaacgacgt
      181 agaacttgcc cgcggtatcg cgatacggcg ttccctcgac cggcgggacg gacgcggcag
      241 gaaccgcgat cagaccaccg aaggccccgg tcttgttcgg ccagagcatg tagaagaagc
      301 cgacgccggc ctcgagcagc acgacaccgg caccgcccaa cgtcgcgttg cggacgaaga
      361 ggcgacggga gtgcagcttg cgggcggcgg acgtgccgtg gcccgcctgg agccgggaaa
      421 gcgtcgccgc gatccgaccc agcgcccaac cccccgcgag gccggccaac gtcttgacca
      481 tcggtgtcat gtcggcgttg atccttactc agtcagtgcg cccggtggtt cgccccacgc
      541 gggacgtctc gccgtgcatt cgaatcgatc attccggggt gcctcggtgc aatcccggat
      601 cagtgatgaa cgagaggcag gtcccacggc aaggtcaggt tctggccgac gccgcggaag
      661 gaggtgccaa agactgtgag gacgatatac gagacgtaga aaccggtgaa gagcgcgatg
      721 atcaattctc gcgtcgtcgg tcggaagatg cctttggtga ggacgacgag aacaccgatc
      781 gcgatcagca cgatgagcgt cgagatcagg atgccgccga cccactcggg ggcgccccag
      841 tgctcctggg tgtagcgacc aaggccgaac tgcgtgccgc gtccgatcgc ttcgtcgaga
      901 aggatcatcc cggagatgac caccgtcgtg tagacggcgg agaaggcgat gatctggcgt
      961 cccttgctgg atgtgccgag gatgccgacg ccgagcgggc tgcgatcgaa gtaggggatc
     1021 gcggcgacgc cggcgagggc gacggttgga agaatcacgc cggcgagacc cgcatccatg
     1081 tgcagcagga gttcctggag attcaggaag taccacggcg ccttggacgg gttcggtgtc
     1141 tggttcggat tcgcacgctc aaggagcggc ccgttcatga ggaccgagag caccgtgagg
     1201 atcgccagga agacgatcgc gcagacggcc tcgacgacga cgagcgacgg ccagaccatc
     1261 acctcgtcgt cggcgagatc ggtgggacgg gccagcgcac gaccgcggac cagctcgagg
     1321 agcttctgcc tcttctgttc gtcgatggcg ttgcccggct ggggagccgg tgggcgttcc
     1381 gcgagttctg ccatatgcga cctgccttct tctcgagtcc tggagcgcga cgctgcgtca
     1441 cagagcgatg tcgaaaggaa ttcctacagg gggccggaga tgcctccgtc cttgcggatg
     1501 cgccagaagt gcacggccat caggaaggcg gcgatcagcg gcaggaagat gacgtgcagc
     1561 gtgtagaagc ggatgagcgt gctctggccg atctgatagt cgccgaggag gatgcggttg
     1621 acctgggggc cgatgatcgg ggcggcgccg ccgatttcgg agccgacggt gatcgcccag
     1681 aacgcgagct gatcccacgg caggagatat cc
//
LOCUS       DASTMD010000276         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2435700, whole genome shotgun sequence.
ACCESSION   DASTMD010000276 DASTMD010000000
VERSION     DASTMD010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2435700"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..938)
                     /locus_tag="VFQ54_04140"
     CDS             complement(117..938)
                     /locus_tag="VFQ54_04140"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEU0164210.1"
                     /translation="MTDPNAPKPMTPEEIDRRIAEIEASLAASTPKPPPAAPAFAEPE
                     TASAPDPQSPPPAFEEPRPRRRPLQADAVFEEDETPPRRESARQESSRAATSFADSQI
                     VERAIGISRFSPRYYADVMADGGATVQAATVVVAVAVASGIGGLGAGLGGFFASLVWA
                     LIKWVLFTVSAWIVARSVFDKKPDGDVTALARVLGFAQAPGLLAIVGFIPFLGWSLAA
                     VGNLWLVLAVVMGLRNVLNLDFRQSFLTAIGAWVAATVIAILLAMVFGGNPGSLF"
     gene            complement(1018..>1508)
                     /locus_tag="VFQ54_04145"
     CDS             complement(1018..>1508)
                     /locus_tag="VFQ54_04145"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEU0164211.1"
                     /translation="QRTYREIAVDPDATRQAAIVVTIVAIASAIGGAADGFSGAVVGL
                     IGAFISWLLFTFVTWFFGTQLFGTPTTNANPESLLRTLGYAQAPSVLGILGFIPILGW
                     VVSVIAGIWVIVTSVFAIRQVLGLSTGRAIITAVIAAIASGIIIGIVGVIFGIGFAIG
                     TL"
BASE COUNT          274 a          490 c          515 g          229 t
ORIGIN      
        1 cgtcccattg tcgatgtctc gcctgtcgcc ccctctccga ccaacgcgcc gtgcgggaag
       61 cacatggacg ccggctggtc tctcgacgag cgcgggatga cggttcggga gactcgctag
      121 aagagcgaac ccgggttgcc gccgaagacc atcgcgagca ggatcgcgat caccgtcgcc
      181 gcgacccagg cgccgatcgc tgtcaggaag gactggcgga agtcgaggtt gaggacgttg
      241 cggagaccca tcacgaccgc gagcaccagc cagagattcc ccaccgccgc gagactccag
      301 ccgaggaagg ggatgaagcc gacaatcgcc agcagcccgg gcgcctgcgc gaagccgagg
      361 acgcgcgcga gcgcggtgac atcaccatcc ggcttcttgt cgaagacgct gcgcgcgacg
      421 atccaggcgc tgaccgtgaa gagcacccac ttgatcagcg cccagacgag gctcgcgaag
      481 aatccgccca gtcccgcccc gaggccaccg attccgctcg cgaccgcgac cgccacgacg
      541 accgtggccg cctggacggt ggcaccgcca tcggccatca cgtccgcgta atagcgcggc
      601 gagaagcggg agattccgat cgcacgctcg acgatctggc tgtcggcgaa cgaggtcgcg
      661 gcgcgactgc tctcttggcg cgcggattct cgccgcggtg gcgtctcgtc ttcctcgaaa
      721 acggcgtcgg cctgaagcgg tcgacgtcgc ggccgcggct cttcgaatgc aggtggcggc
      781 gattgcgggt cgggggcgct cgcggtctcc ggttcggcga atgctggggc ggcgggaggc
      841 ggtttcggtg tgctcgcggc cagcgaggcc tcgatctcgg cgatccggcg atcgatctcc
      901 tcgggtgtca tcggtttcgg ggcgttcgga tccgtcatgc ggggcgactc cttctggtac
      961 ggaaatgata acggcggcac cccggattcg ggatgccgcc gtctgcaatt cggagattta
     1021 cagcgtgccg attgcgaatc caatcccgaa gataacgccg acgatgccga tgatgatccc
     1081 gctcgcgatc gccgcgataa cggcggtgat gatcgcccgc cccgtactga gaccgagaac
     1141 ctgccggatc gcaaagaccg aggtcacgat cacccagata ccggcgatga ccgaaacgac
     1201 ccagcccagg atcgggatga agccgaggat tccaaggacg ctcggcgcct gggcatagcc
     1261 gagcgtgcgg agcagggatt ccgggtttgc gttcgtcgtc ggcgtgccga agagctgggt
     1321 tccgaagaac caggtcacga aggtgaaaag cagccagctg atgaacgcgc cgatcaagcc
     1381 gacgacggca ccgctgaaac cgtctgccgc gccaccgatc gcgctcgcga tcgcgacgat
     1441 cgtgacgacg attgcggcct ggcgtgtggc gtcgggatcg accgcgatct cgcggtaggt
     1501 ccgctgta
//
LOCUS       DASTMD010000277         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3060520, whole genome shotgun sequence.
ACCESSION   DASTMD010000277 DASTMD010000000
VERSION     DASTMD010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060520"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFQ54_04150"
     CDS             <1..212
                     /locus_tag="VFQ54_04150"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEU0164212.1"
                     /translation="TRAFKGCLAVDVVQNVDDPTHVQFIEKWESLEDVVAYRAWRAGD
                     GATPETAAFVAAPPTATNLNLLEDI"
     gene            complement(259..669)
                     /locus_tag="VFQ54_04155"
     CDS             complement(259..669)
                     /locus_tag="VFQ54_04155"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEU0164213.1"
                     /translation="MAMAQATTGKLTLLFDGNCGFCTRTVLWVQAKDTQGRIDVIPCQ
                     VAVRNGTHDVSEGDCGKSIWAFTAEGHVEGGAQAAALVASELMGRRWPVQVAKLPVIK
                     QSLDLGYRFIANNRHRLPGIKGMCAIDGGSSCRS"
     gene            complement(925..>2012)
                     /locus_tag="VFQ54_04160"
     CDS             complement(925..>2012)
                     /locus_tag="VFQ54_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0164214.1"
                     /translation="HRKLALHPLMHVAGMLRAAIACGGRGLRLVTMKDIAVRAGVSMI
                     TVSRVVNNSGYVGAETRRRVEAAIEELHYVPNLVASNLRSQQSDLLALLLPDITNSFW
                     TSIARGVEDEAWTRGYGVFICNTDNNLLKEESYVERILRRRVEGVLVVPTPDPASELQ
                     LARLRRQGVKFVVIHRRLLGKEADVVRSDGLEAARRLTEELVKTGRRRIAFVGMPLED
                     PAMQDRLAGFRAALKAAGVPPDESLIRVGAIHTEIGGYGAVKELLRVATPPDALLIAN
                     SRLALGGLRAIADAGLRIPEDISVAAFHDINLMDPWASRLIRAVQPSYRMGQLATRRL
                     LEIETTEDTGFQEIILEPDIHLPNEPF"
BASE COUNT          356 a          694 c          612 g          350 t
ORIGIN      
        1 acacgcgcgc cttcaagggg tgcctcgccg tcgatgtcgt gcagaacgtc gacgatccca
       61 cacacgttca gttcatagag aaatgggaat cactcgagga cgtcgtcgcc taccgcgcgt
      121 ggcgcgcggg tgatggcgcg acaccggaga ccgcggcgtt tgtcgccgcg ccaccgaccg
      181 cgacgaatct gaacctgctc gaggacatct agaccgtcac cgccgggcca cgacacgtcg
      241 tggcccggcg cccggggatc aggatcggca gctgctcccg ccgtcgatgg cgcacatgcc
      301 cttgatgccg gggaggcggt ggcggttgtt ggcaatgaac cggtagccga gatcgagcga
      361 ctgcttgatc accggcagct tcgcgacctg caccggccag cggcgtccca tcagctcgga
      421 tgcgaccagc gccgccgcct gtgcgccacc ctcgacgtga ccctcggctg tgaacgccca
      481 gatactcttg ccgcaatcgc cctcgctcac atcatgggtt ccgttccgca ccgcgacctg
      541 acacgggatg acgtcgatcc gcccctgcgt gtccttcgcc tgcacccaca gcacggttcg
      601 cgtgcagaag ccacagtttc cgtcgaagag gagggtcagt ttcccggtgg ttgcttgggc
      661 catcgccatc acaggtcatc cattcgtgcg aatcgttccg cgggcgccgc aagacgtaac
      721 ccgcttcgcc ctggtcagag tcgggagcta tccccgccag gacaacgacg atatatttgc
      781 gtgtattcta catcactccg cgtcgcgttt ccgtcggata tcgacgttct cgagcggtca
      841 acccgtctct tcgcgacggg tcaaggcgtt cgctgtcgag gcacgaatcc atgcccggtg
      901 gatcatcccg agaggtccgg cgacctagaa cggctcgttc ggcaagtgga tatccggctc
      961 gaggatgatc tcctggaagc ccgtatcctc ggtcgtttcg atctcgagca gacgacgcgt
     1021 cgccaactgt cccatacgat aggacggctg caccgcgcgg atcagtcgtg acgcccatgg
     1081 gtccatcagg ttgatgtcgt ggaaggcggc gacggagatg tcctccggga tccgaagccc
     1141 ggcatcggcg atcgcgcgca agccgccgag cgcgagccgg ctgttcgcga tcagcagcgc
     1201 gtctggcggt gtcgcgaccc gaagcagttc cttcaccgcg ccgtagccgc cgatctcggt
     1261 gtgaatcgca ccaacccgga tcagtgactc atccggcggg acccccgccg ctttcagggc
     1321 cgcccggaac ccggcaaggc gatcctgcat cgccgggtcc tcgagtggca taccgacgaa
     1381 cgcgatccgt cgtcgccccg tcttcaccag ctcctccgtc aggcggcgcg ctgcctccag
     1441 cccgtcactg cgaacgacat cggcctcctt accaagcagg cggcgatgga tgacgacgaa
     1501 cttcacaccc tgccgtcgca gccgcgcgag ctgaagctca ctcgcgggat ccggcgtcgg
     1561 cacgacaagc acgccttcga cgcgacgccg caggatgcgc tcgacgtagc tctcttcctt
     1621 cagcaggttg ttgtcggtat tgcagatgaa gacgccatac ccccgcgtcc acgcttcgtc
     1681 ctcgacgcca cgcgcaatcg aggtccagaa ggagttcgtg atatccggca ggagcagggc
     1741 gagcagatcc gactgttgcg atcgcagatt gctcgcgacc aggttcggga cgtagtgaag
     1801 ctcctcgatc gcggcctcga ccctgcgccg cgtctccgcg ccaacatacc cggaattgtt
     1861 caccacccgc gacacggtga tcatggagac cccggcacga acagcgatat ccttcatcgt
     1921 aaccagccgc agcccccttc caccgcacgc gatagctgcg cgaagcatac ccgcgacgtg
     1981 catgagcggg tggagggcca gtttgcgatg gg
//
LOCUS       DASTMD010000278         1874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2703283, whole genome shotgun sequence.
ACCESSION   DASTMD010000278 DASTMD010000000
VERSION     DASTMD010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703283"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VFQ54_04165"
     CDS             complement(<1..861)
                     /locus_tag="VFQ54_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0164215.1"
                     /translation="MTATIAASRLPERHLHVHDLGKRYHDTGSASDWALRNLTLDVPF
                     GSLVALLGANGAGKSTLIHLLSGAIAPTTGFIGELSPDTTLGWCSQRSSIDWYLNVWQ
                     NVRMGARLAGHGIAASRVLTERALEIVGLTGKATANVDALSGGQQQRLQIARALVANP
                     DILLLDEPTVGLDVESSERLLADLRRRADDGALVIVSSHDLGLLEAWCDRVLLIAEGR
                     LIAFESHAAFMTRFGGEEVLEIAIGGDLPTAAILRLGQPGSGVRLIEATPLRLGIPRG
                     TPLGRILALLE"
     gene            1334..>1874
                     /locus_tag="VFQ54_04170"
     CDS             1334..>1874
                     /locus_tag="VFQ54_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164216.1"
                     /translation="MHATRLLSRWGLVLALLFATFGQIAALGSTSAAAATQAEDPYPG
                     PSSTPKVRDTACPFSDADIPSDIAARGNLACGTVDVPEDHANPDGKSISVFIVQIPSI
                     SKKPNSSPLFVLAGGPGQGSSGQLGQFSKTVQDPIASWYPFLKDHNVVLIDQRGTGYS
                     DPNLVCPADLPATPGATPVS"
BASE COUNT          338 a          555 c          651 g          330 t
ORIGIN      
        1 ctcgaggagg gcgaggatcc gaccgagcgg ggttccccgg gggatgccaa gacgaagggg
       61 cgtcgcttcg atgaggcgaa caccgcttcc gggctgaccg aggcgcagga tcgcggcggt
      121 cgggaggtcc ccaccgatcg cgatctcgag aacctcctcg ccaccgaagc gcgtcatgaa
      181 ggcggcatgt gattcgaacg caatgagccg gccttccgcg atcaggagca cgcgatcgca
      241 ccaggcttcg agcaggccga gatcgtgaga ggagacgatc accagggcgc cgtcatccgc
      301 acggcgacgc agatccgcca gcaatcgttc cgacgactcg acatcgagac cgactgtcgg
      361 ttcgtcgagg aggagaatgt cgggattggc gacgagcgcc cgcgcgatct ggagacgttg
      421 ctgttgaccg cctgagagcg cgtcgacgtt ggcggttgcc ttgccggtga gaccgacgat
      481 ctcgagagcg cgttcggtga ggacgcggct ggcagcgatg ccgtgcccgg cgaggcgggc
      541 gcccatccgc acgttctgcc agacgttgag ataccagtcg atgctggagc gttggctgca
      601 ccaacccagg gtggtatccg gcgaaagttc gccgatgaat ccggtcgtcg gcgcgatcgc
      661 tccgctgagg agatggatga gggtggattt tccggcgccg ttcgcaccga ggagggcgac
      721 gagcgagccg aacggcacgt cgagggtcag gttccgaaga gcccagtcgg acgcggagcc
      781 ggtgtcgtgg tagcgcttgc cgagatcatg gacgtggaga tggcgttccg ggaggcgtga
      841 agcggcgatg gttgcggtca tacgatcctc gttctggcat ggaaagtccg gaagtgaggc
      901 tatccatgcg aagatcgtcg tagaacaaca tgcgcgggca tcccttgtct gaacggggag
      961 tcgatggtcc tgagtctagc gaccggtgag agggaacgca atggggatgt tgccgtgaat
     1021 cgggacaccc gtcctgccgc gggatggatg catggggcaa ggcgagggat ccggggaagt
     1081 tcgggatttc attctggaga gacttgcggc agatgcgtcc ggaactcatc attccggaag
     1141 tgattcaggt gaaccggcgg gccgtcttct gcgttatgga ttcaggatgg gtatccccac
     1201 gccagcgcgt tgctggggcg ggatcggttg aagacggaat cgccattcgc agacgcaggt
     1261 cgcaatcacc gggtggctgg ccatatttcg ccaggcggcg cttcgctcgc cggcaagagt
     1321 taaggatgcc ttgatgcacg ctacccgtct tctcagccgg tggggacttg tcctcgcgct
     1381 gctgttcgcc acgttcggcc agatcgccgc gcttggcagc accagtgccg cggccgcaac
     1441 ccaggccgag gatccctatc cgggaccctc gagcacgccg aaagtccgcg acaccgcctg
     1501 tcccttctcg gatgcggata tcccgtccga tatcgccgcg cggggcaatc tcgcctgtgg
     1561 cacggtggat gtcccggagg atcacgccaa tccggatggg aagtcgatct cggtcttcat
     1621 cgtccagatc ccgagcatca gcaagaagcc gaattcgtcg ccactcttcg tgctcgccgg
     1681 tgggcccggc cagggatcat cggggcaact cgggcagttc agcaagacgg ttcaggatcc
     1741 gattgcctcg tggtatccgt ttctcaagga tcacaacgtg gtcctcatcg accagcgcgg
     1801 cacgggctat tccgacccga acctggtctg cccggccgat ctcccggcga ccccgggcgc
     1861 tacgccggtc tcgg
//
LOCUS       DASTMD010000279         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3149890, whole genome shotgun sequence.
ACCESSION   DASTMD010000279 DASTMD010000000
VERSION     DASTMD010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3149890"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFQ54_04175"
     CDS             complement(<1..378)
                     /locus_tag="VFQ54_04175"
                     /inference="COORDINATES: protein motif:HMM:NF017812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 family protein"
                     /protein_id="HEU0164217.1"
                     /translation="MVESLTAKSSVLSSQLQEITVDQLEALAIGAGILGTGGGGNPYL
                     GKIFTRGLLAGGGSVPVVALVDVPDDAVVVSVGGIGAPVVGVERLKEGSEFLRAMRAL
                     EHHTGKTATHMIAAEIGGGNSLAP"
     gene            617..>2158
                     /locus_tag="VFQ54_04180"
     CDS             617..>2158
                     /locus_tag="VFQ54_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEU0164218.1"
                     /translation="MSLRIGVDVGGTNTDAVLMDDRLVIAKLKTPTTEDVTSGITTAI
                     RSVLEQSGTATEQIASVMIGTTHFTNAIVERRHLSPVAAIRLGLPATAALPPMIDWPE
                     DLLETLPHHVYLCHGGHEFDGRIISPFDADEIRGVAKEIAAKGVTAVAISSVFSPVNA
                     EMEDRAAVIVEEEIPGVSITKSNEIGRIGLLERENAAILNSCLGELASRTIAAFERAI
                     SELGITAPLYLTQNDGTLMAADFARIYPVLTIASGPTNSMRGAAFLSGLTDAIVVDIG
                     GTTSDVGMLTHGFPREASVAVEVGGVRTNFRMPDVYSIGLGGGSHVNFETMQIGPQSV
                     GYRLTEKALIFGGDTLTTSDIAVASGIVEMGDAAKVAGLDSTQVAAALDKIQEIVAAA
                     VDRMKTSAEAVPVIVVGGGSVLISRPVEGASEMVKPNHFEAANAVGAAIAQISGEVDR
                     VYALDGLTRDEAVQSAKDEASDRAVAAGADPATIQIVDVEDVPLAYLPGNATRIRVKA
                     VGDLTL"
BASE COUNT          385 a          729 c          666 g          378 t
ORIGIN      
        1 cggggccaac gagttcccgc cgccaatttc cgcggcgatc atgtgcgttg cggtcttccc
       61 ggtgtggtgc tccagcgccc gcatcgcgcg gaggaactcc gacccttcct tgagacgctc
      121 gacaccgacg accggcgcac cgatgccacc aaccgacacg acgaccgcgt cgtcagggac
      181 gtcgaccagg gcaacgacgg gcacgcttcc gccacccgcg agaagcccgc gcgtgaaaat
      241 cttgccaaga tagggatttc cgccgccacc cgtgccgagg atgccggcac caattgccaa
      301 cgcttcgagc tgatcgaccg taatttcctg aagttgtgac gagagtacgg acgatttcgc
      361 ggtcagagac tctaccattc gtgatccacg ctccagaact gacctgcgcg ctccctgagc
      421 cctcagaaag ccgtcaactt cggaatctta cgcgcgcgtt cgcagatttc aattggacgt
      481 tgcgcacaga cgcgttcgtt attttgacca acgcgatttt tcatcgccac ccgtatcatt
      541 gcgcgaggaa tcgacgtgcg cgacgcaatg cgcccttctt cccctcacca acgtccttca
      601 attggagatt ctgaagatga gtttgcggat tggcgtcgac gtcggcggca cgaatacaga
      661 tgcggtcttg atggatgatc ggctcgtgat cgccaagttg aagacgccca ccacggaaga
      721 cgtgacgagc ggaatcacca cggcgatccg gagcgtgctc gagcagtccg gcaccgcgac
      781 tgaacagatc gcctcggtca tgatcggcac cacccacttc accaacgcga tcgtggagcg
      841 acgccatctc tcgccggtcg cggcgattcg cctcggtctc cccgcaacgg ccgcgctgcc
      901 cccgatgatc gactggcccg aggatctcct cgagacgctt ccccaccacg tctatctctg
      961 ccacggtggt catgaattcg acggtcgcat catctccccg ttcgacgccg acgagattcg
     1021 cggcgtcgcg aaggagatcg ccgcgaaggg tgtcaccgcc gttgcgatct cgagcgtctt
     1081 ctccccggtc aacgcggaga tggaagatcg cgccgccgtg atcgtcgagg aggagatccc
     1141 cggcgtctcg atcacgaagt cgaacgagat cggtcgcatc ggcctcctcg aacgtgagaa
     1201 cgcggcgatc ctcaactcct gcctcggtga gcttgccagc cgcaccatcg ccgccttcga
     1261 gcgcgcgatc tcggagctcg gaatcacggc gccgctctat ctcacccaga acgacggcac
     1321 gctgatggcc gccgatttcg cgcgcatcta tccggtcctc acgatcgcct ccggccccac
     1381 gaactcgatg cgcggcgcag cgttcctctc cggtctcacc gacgcgatcg tcgtcgatat
     1441 cggaggcacg acctccgacg tcgggatgct gacgcacggc ttcccgcggg aagcgtcggt
     1501 cgcggtcgag gttggcggcg tccgcaccaa cttccggatg ccggatgtct actcgatcgg
     1561 cctcggcggt ggatcgcacg tcaacttcga gacgatgcag atcggcccgc agagtgtcgg
     1621 gtatcgcctc actgagaagg cgctcatctt cggtggcgac acgctgacga cctcggacat
     1681 cgcggtcgcc tcgggcatcg tcgagatggg tgacgccgcg aaggtcgccg gtctcgattc
     1741 cacgcaggtt gcggcggcgc tggacaagat ccaggagatc gtcgcggcgg cggtcgatcg
     1801 gatgaagacg agcgccgagg ccgtcccggt gatcgtcgtc ggtggcggca gcgtcctcat
     1861 ctcgcgcccg gtcgagggtg cgtccgagat ggtgaagccg aatcacttcg aggcggcgaa
     1921 cgccgtcggc gccgcgatcg cccagatctc gggcgaggtc gaccgcgtgt acgcgctcga
     1981 cggcctcaca cgtgacgagg ccgttcagtc ggcgaaggac gaagcgtcgg accgcgccgt
     2041 ggcggcggga gccgatcccg cgacgatcca gatcgttgat gtcgaggacg tgccgctcgc
     2101 ctacctgccc ggcaacgcga cgcgaattcg cgtcaaggcc gtcggcgacc tgacgctc
//
LOCUS       DASTMD010000280         1903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_561747, whole genome shotgun sequence.
ACCESSION   DASTMD010000280 DASTMD010000000
VERSION     DASTMD010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561747"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFQ54_04185"
     CDS             complement(<1..743)
                     /locus_tag="VFQ54_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU0164219.1"
                     /translation="MERRVKIVTTLGPAVIPSAENPGRLEELMAAGADVVRINAAHGA
                     VEFRARLVRDVRAAAAAVGKRVPILLDLQGLKIRTGPLAEGSAPVPFARGSSVKLLPN
                     PDVTKDGQIGINYADLLKVVSPESRVLIADGLIELLVEKVNSDHAIAVVSRGGLLLGR
                     QGVTLPGAPIKGGALTEADRVDVKFAVEHGIDYLGLSFINDAGDLALTREVARSCDGP
                     VPGIISKIERPEALNNLREIAGSSDGIMVA"
     gene            complement(947..>1903)
                     /locus_tag="VFQ54_04190"
     CDS             complement(947..>1903)
                     /locus_tag="VFQ54_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0164220.1"
                     /translation="VTRMINPKNDKSISKNVTEMDGGFLDNANLTFQSRANGYDSDAA
                     IVDALKNDPDVAVVDSSALSANAGFSGDEQLTIPALPDKGSFDAPTLTVYPSAGQTKT
                     VKVIGVIDQKISSLAGVFVGPVTGQQMFPKPGATSTSYFIKLEDGASAKTVADNVERA
                     LIANGAQGVDIEQELKDNQRQSQSFLYILQGFMGLGLIVGVAAVGVIAYRAIVERRQQ
                     IGMLRALGFQRSVISQAFVMESAIVVVIGVVAGAIFGLALSYNLMTSDDFTNGADAPS
                     FIIPWPTMIVVLVASVVAALLMAWVPARQASRVVPAEALRYE"
BASE COUNT          345 a          606 c          606 g          346 t
ORIGIN      
        1 gcaaccatga tgccgtcaga actgccggcg atctcgcgga ggttgttcag tgcctccggg
       61 cgctcgatct tcgagatgat gccgggcacg gggccgtcgc aggagcgggc cacctcacgc
      121 gtcaacgcga gatcgccggc gtcgttgatg aagctgagac cgagatagtc gatcccgtgt
      181 tcgaccgcga acttgacgtc gacccggtcg gcttcggtaa gggcgccccc cttgatcggt
      241 gcgcccggga gggtcacgcc ctggcggccg agcagcaggc caccgcgact gacgacggcg
      301 atcgcgtgat cggagttgac cttctcgacg aggagttcga tcaggccgtc ggcgatcagg
      361 acgcgtgatt ccggcgagac caccttgagc agatcggcgt agttgatgcc gatctgtcca
      421 tccttcgtca cgtccgggtt cgggagaagc ttgacgctgc tgccccgtgc gaagggcacg
      481 ggcgcgcttc cctccgccag cggtccggta cggatcttga ggccctgaag atcgaggagg
      541 atcggcacgc gcttcccgac ggcggcggcg gcggcgcgga catcgcgtac gagcctggcg
      601 cggaattcta cggccccgtg cgcagcattg atgcggacta cgtcggcgcc tgcggccatc
      661 agctcttcga ggcgaccagg attctcggcg gacgggatga ccgcgggccc gagagtcgtg
      721 acgatcttga cacgtcgttc catgaaagga tgctccagtg catttcctga ttgatgactt
      781 cgggacatcc tcatcccgtc ctcatcaagc caacccacat ggtagcgcat tggtcaccgc
      841 gaatggctaa aggaagagcg tgcagaagcc aacaatcgca aaaacgacgg acgtcgagga
      901 aatccccggc gcccgccgtc cagcgatcac gttgttccgt aaagctttac tcgtatcgga
      961 gtgcctccgc gggaacgacg cgggacgcct gcctcgccgg tacccacgcc atcagcaggg
     1021 ccgccacgac cgatgccacg aggaccacga tcatcgttgg ccaggggatg atgaacgatg
     1081 gtgcgtccgc gccgttggtg aagtcgtcgc tcgtcatcag gttgtagctg agggcgagcc
     1141 caaagatcgc gcccgccacg acgccgatga cgacgacgat cgccgattcc atcacgaatg
     1201 cctgagagat cacgctccgc tggaagccga gcgcccgcag catgccgatc tgctggcgcc
     1261 gctccacgat cgcacggtac gcgatcactc cgaccgccgc gacaccgacg atcaggccga
     1321 gtcccatgaa tccttgcagg atgtagagga acgactgcga ctggcgctgg ttgtccttca
     1381 gttcctgctc gatatcgacg ccctgggcgc cgttggcgat cagcgcgcgc tcgacgttgt
     1441 cggcaaccgt cttcgcgctc gcgccatcct cgagcttgat gaaataggac gtcgatgtgg
     1501 cgcccggctt cgggaacatc tgctgtcccg tcaccggacc gacgaagaca ccggccaggc
     1561 tcgagatctt ctggtcgatg acaccgatga ccttcaccgt cttcgtctga ccggcactcg
     1621 ggtagaccgt cagcgtcggc gcatcgaacg atcccttgtc ggggagggcc gggatggtga
     1681 gctgctcgtc cccactgaaa cccgcattgg cggaaagcgc actgctgtcg acgaccgcga
     1741 catcgggatc gttcttcaag gcatcgacga tcgcggcgtc ggagtcgtag ccgttcgccc
     1801 gggactggaa tgtcaggttc gcgttgtcga ggaagccgcc gtccatctcc gtgacattct
     1861 tcgagatcga cttgtcgttc ttcggattga tcatccgggt tac
//
LOCUS       DASTMD010000281         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2167801, whole genome shotgun sequence.
ACCESSION   DASTMD010000281 DASTMD010000000
VERSION     DASTMD010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2167801"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            390..863
                     /locus_tag="VFQ54_04195"
     CDS             390..863
                     /locus_tag="VFQ54_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0164221.1"
                     /translation="MSDLSEGDLRSTARELRSVLGLLMRRLRAEGPRQDLSWSQDSAI
                     SRLDRFGPSSISELARAEGVRSQSMGATITALEGEGLVSRSPDPEDGRQSVISLTGAG
                     RHALHRARVERQEWLIEAMSARLTPEEQGKVAEAIELMHRMLEYPEREHSDRDLD"
     gene            983..1543
                     /locus_tag="VFQ54_04200"
     CDS             983..1543
                     /locus_tag="VFQ54_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU0164222.1"
                     /translation="MAVTAIDPKTALVVIDLQKGIMTYPTVHPATEIVARSAELAKEF
                     RRLGLPVILVNVNGGAPGRTEQSRSVGERPADWADLVPELDAEPTDYLVTKQTWGAFY
                     NTSLHEYLQAQGVTQIVLTGISTSAGVESTARSAYEHGYNVTIATDAVTDMVPESHRH
                     SVERVFPRLGETGTTAEIIELLARGR"
     gene            1581..>2424
                     /locus_tag="VFQ54_04205"
     CDS             1581..>2424
                     /locus_tag="VFQ54_04205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164223.1"
                     /translation="MAAQQSTAHSGLERSPFDWRFLTPLLLGAVINPVNSSIIATALV
                     GIGRDLHASASSTASLVAGLYLACAIAQPTMGKLARHFGARRIFLLGLVIVAIGGIIG
                     TAAPNLGTLLVSRVLIGIGTSAGYPTAMALIRQRADDSRMGIPGSALGSIAIAGQATV
                     ALGLPLGGVIVGLAGWRSTFLLNVPLAIVGIIVTLLWIPEDRRREGPRESMVAAIDPL
                     GIVLFGGAITSLLIFLQGLKSPTWWLVPIVIACSVLLIVWERRAPAPFIDVPMLAKNA
                     PLRRT"
BASE COUNT          380 a          801 c          798 g          445 t
ORIGIN      
        1 agcggctccc tggtctggcg atcgccggca acatgttccg cggggtcggc tttccggatt
       61 gcattcgctc cggcgaggat gccgcggatg cagttctcgc cgggatcgca cgcccggcgg
      121 cagacgaaat tgcgccgaaa gccgccgtcg catcctgagc gaggccgaac gaatcgaggg
      181 cccacggtcc ccgttcccgg cggcgccgct ccgcctgggt agcgccggcc cggcatcaat
      241 gttccgcctc atgccggcca cgaacgcctt gctggcagct gcctcggagg aacccgtacg
      301 ggtgccgacg acgcggcgcg catgcagtcg tgtctcggga tgtgaaggaa agcttcgtag
      361 tctggcttca tatctggtac gatatgaaca tgtctgatct ctccgagggc gacctccgat
      421 ccaccgctcg tgaacttcgt tctgtccttg gcctgctcat gcggcgcctg cgcgcggaag
      481 ggccgcggca ggacttgagc tggtcgcagg attccgccat ctcgcggctg gaccgcttcg
      541 gcccgtcatc gatctcggaa ctggcccggg cggaaggcgt tcgctcccag tcgatggggg
      601 cgacgatcac cgccctggaa ggcgaagggc tcgtcagccg gagtcctgat cccgaggacg
      661 gtcggcagag cgtgatctcg ttgaccggcg ctgggcgtca tgccctccac cgggcgcggg
      721 tggagcggca ggagtggttg atcgaggcga tgtcggcccg gctcacgccc gaggagcagg
      781 ggaaagtcgc cgaggcgatc gaactgatgc accgcatgct ggagtatcca gagcgggagc
      841 attcggaccg cgacctcgac tagaaccttc aggctgcgac cagattcgtt ttcccgattg
      901 atgtttccga tcggcggcct cgcctgttcg cgaggctcgc ccgaccgtgc gtgcgtcgac
      961 tgacgcgaag gagtttgctt ggatggctgt gaccgcaatt gatccgaaga ccgcactcgt
     1021 cgtcatcgat ctgcagaagg gcatcatgac ctatccgacg gtgcatcccg cgaccgagat
     1081 cgtcgcccgg tcggcggaac tcgcgaagga atttcgccgc ctcggactgc cggtcatcct
     1141 cgtcaatgtg aacggtggtg cgccgggccg caccgagcaa tcgcgatcgg ttggggagcg
     1201 accggctgat tgggcggatc tcgtgcccga gctcgatgcg gagccgaccg actatctcgt
     1261 cacgaagcag acgtggggcg ccttctacaa cacatcgctc cacgaatacc tgcaggcaca
     1321 gggcgtgacc cagatcgtgc tgacgggaat ctcgaccagc gctggcgttg aatccacggc
     1381 gcgatcggcc tacgagcatg gctacaacgt cacgatcgcg accgatgcgg tgaccgacat
     1441 ggttcccgaa tcccaccggc acagcgtcga gcgggtcttt ccccgcctgg gcgagacggg
     1501 cacgacggcg gaaatcatcg agttgctcgc ccggggtcgt taggatctct cgcgtgacgt
     1561 gatctggatc gagaacaccc atggcagcgc aacaatccac cgcgcacagc ggactcgaac
     1621 ggtcaccgtt cgactggaga ttcttgaccc cgctcctgct tggcgctgtc atcaatccgg
     1681 tcaacagctc gatcatcgcg acggctctcg tcgggatcgg tcgggatctt cacgccagcg
     1741 cttcgtcgac ggcgtcgctc gtcgccggcc tttacctcgc ctgcgcgatc gcccagccga
     1801 cgatggggaa gctggcgcgt catttcggag cgcgccggat cttcctcctc ggtctggtca
     1861 tcgttgcgat cggcgggatc atcggcacgg ccgcgccaaa tctcgggacg ctgcttgtct
     1921 cgcgtgtcct gatcggaatc ggcacctcgg ccgggtatcc caccgcgatg gcgctgatcc
     1981 ggcagcgcgc cgacgatagc cggatgggta ttcccggcag cgcgctcggg agcatcgcaa
     2041 tcgccggcca ggcgacggtc gcgctcggtc ttcccctcgg tggcgtgatc gtcgggctgg
     2101 ccgggtggcg atcgaccttc ctgctcaacg tgccactcgc gatcgtcggc atcatcgtca
     2161 cgctcctctg gattccggaa gaccgccgtc gcgaggggcc gcgcgaatcg atggtcgccg
     2221 cgatcgaccc gctgggcatc gtgctcttcg gcggcgcgat cacgagcctg ctgatcttcc
     2281 tccaggggct gaagtcgccg acctggtggc tcgtcccgat cgtcatcgcc tgcagcgtgc
     2341 tcctgatcgt ctgggagcgg cgggctcccg cgcccttcat cgatgttccg atgctcgcca
     2401 agaacgcgcc gttgcgccgg acct
//
LOCUS       DASTMD010000282         1693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2882116, whole genome shotgun sequence.
ACCESSION   DASTMD010000282 DASTMD010000000
VERSION     DASTMD010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2882116"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="VFQ54_04210"
     CDS             complement(<1..1165)
                     /locus_tag="VFQ54_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEU0164224.1"
                     /translation="MKTFLRILSYSKPYWRNLAFVYAALFLALAFQLTIPDFLGRAID
                     RGVQGRDHTYLWHAALIIVGLAFIQAIFTYVRSYGTNVLAERVGCDIREELYGKFQEL
                     PFQFYDRSQSGQLMARATEDINSIRNMMMFAIRAIVQAAGMLVIVAVILFQKDWRLAL
                     VALSTTPILMWWSVRFGISIRPVFLRIQQQFGAMTSVLQENVAGGRVVRAFAQERAES
                     ERFEEELEELFKRNLRAANRWAFNYPMTLALNGISIAGVVWFGGYLVLTDRMSIGTLV
                     AFQIYTTMLQEPIRWLGFVVQRVARANASGDRIFEILDTKAAIRDLPDARPLDSMRGV
                     VRFENVDFHYVGGKGDALRGVSFTAEPGQIVALVGPTGSGKSSIVSLIPRFYDV"
BASE COUNT          370 a          517 c          517 g          289 t
ORIGIN      
        1 tcacgtcgta gaagcgcggg atcaggctga cgatcgagga cttgccggaa cctgtcggtc
       61 cgacgagggc gacgatctga ccgggttccg cagtgaagct gacgcccctg agtgcgtcac
      121 ccttgccacc cacatagtgg aaatcgacgt tctcgaagcg gacgacgccc cgcatcgaat
      181 cgagcggtcg agcgtccggc agatcgcgga tcgccgcctt cgtatcgaga atctcgaaga
      241 tacgatcgcc ggaggcgttc gcgcgcgcca cgcgctggac gacgaatccc agccagcgaa
      301 tcggctcctg cagcatcgtc gtgtagatct ggaatgcgac gagcgttccg atcgacatcc
      361 tgtcggtcag gacgaggtag ccaccgaacc agacgacgcc ggcgatgctg atgccgttca
      421 aggcgagcgt catcgggtag ttgaaggccc agcggttggc ggcgcggaga tttcgcttga
      481 agagttcctc gagttcctcc tcgaagcgct cggattccgc tcgctcctgc gcgaaggcgc
      541 ggacgacgcg gccaccggcg acgttctcct ggaggacgga tgtcatcgcg ccgaactgct
      601 gctggatgcg gaggaagacc ggtcggatcg agataccgaa acggaccgac caccacatca
      661 ggatcggcgt tgtcgagagc gcgacgagcg cgagtcgcca gtccttctgg aagaggatga
      721 cggcgacgat caccagcatt cccgccgcct gcacgatggc gcggatcgcg aacatcatca
      781 tgttccggat gctgttgatg tcttccgtcg cccgggccat cagctgaccc gactgcgagc
      841 gatcgtagaa ctggaacgga agctcctgga actttccata gagctcttca cgaatgtcgc
      901 agccaacccg ctccgcgagg acattggtcc cgtaggaacg cacatacgtg aagatcgcct
      961 ggatgaacgc aagcccgacg atgatcagcg ccgcgtgcca gaggtatgtg tggtcgcgcc
     1021 cctggacgcc acggtcgatc gcgcgaccga ggaaatcagg aatcgtcaat tggaaggcga
     1081 gggccagaaa gagcgccgca tagacgaaag ccaggttccg ccaataaggt ttcgagtacg
     1141 agaggatacg aagaaaggtt ttcacaaaga tgcctcgccg gtgatgtacg tgcggatccc
     1201 gaatccgccg ccgcctcgcg ccgggcgccg aacatcgccg gcgtcgaccc tggtactggt
     1261 aaatggagga gaacccgctc tcccatggac catcgtgatc ccgcccgcgg gggttgctgc
     1321 aggaagagcc accttccctg cgtacatagg ggcgcagtgt acgaaattcg ggggaattgt
     1381 aaaccccccg gtctcatcat ttctcacatc atcgttgctt aacacggcag cagcgccgca
     1441 atccctcggc atcatgcagc ggcgggatgc ggaaagcgtc aaggcattct gacacgcctt
     1501 tcccgatccg tcgcccacga accacctcaa gtgagtcagc tccctttcgg caggacgcga
     1561 ctccatgccc agtccggcga agcatcagaa cagatgtccg aaacatatga acgaacagta
     1621 ttgacaccac gtcacactcg tgagaacatg tgttcgtaca cagagaacgg gttgcgaatg
     1681 cagcctccgg gca
//
LOCUS       DASTMD010000283         1938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1543609, whole genome shotgun sequence.
ACCESSION   DASTMD010000283 DASTMD010000000
VERSION     DASTMD010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543609"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1938
                     /gene="dnaE"
                     /locus_tag="VFQ54_04215"
     CDS             <1..>1938
                     /gene="dnaE"
                     /locus_tag="VFQ54_04215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU0164225.1"
                     /translation="ACLGGPVANNFLNGAPEKALSYAGSLSEIFGPDRFFIEIQDAGL
                     KEQVQNNKDLIEVARKFNLPLVATNDVHYCFEEDAPAQDVLVCVQTNTTINDAKRLKS
                     ETNQLYLKSPQQMEQLFGSVPEALSNTIRIAEMCALDLGFKGYQLPEFDVPAGFTPET
                     YLRKLCEEGAMKHYGTLDGTIGERLSYELGVIGSMGFTNYFLVVWDFVLFAKQNGILV
                     GPGRGSAAGSLVTYCLDITGLDPLKYDLIFERFLNPSRISMPDIDIDFADDRRDEVIK
                     YVVQKYGDDRVAQIVTFGTLKAKAAVRDVGRAMGLSFGDTDRVAKLIPTDPKMTINLA
                     MSQVPELSKLYDGDGQVKELIDTALKVEGLARHSSTHAAGVVISRDPLVQHVPLQRAG
                     GKSEGDVTTQWGQGHLEDLGLLKMDFLGLKTLTVIGKAVDLARESGSDISIETIPMDD
                     QKAFELLGKGETVGVFQLEGGMTTRMTVDVAPSTFEDIIALMALIRPGPMEMAPDFIA
                     RKHGRAEIAYDHPILENILKETYGVALYQEQVMQIANVLAGFSMAEADGLRKAMGKKQ
                     PEIMAKYRERFIEGSVELGIEKKLAGSVYDMIEKFAGYGFNKAHSAGYAVIAAQTAYL
                     KANYPVQFMAALMSTEIGNTDK"
BASE COUNT          402 a          591 c          611 g          334 t
ORIGIN      
        1 gcctgcctcg gcgggcccgt ggcgaacaac ttcctcaacg gggctcccga gaaggcgctc
       61 tcgtacgccg gttcgctcag cgagatcttc ggacccgatc gtttcttcat cgagatccag
      121 gatgccggtc tcaaggaaca ggtccagaac aacaaggacc tgatcgaggt cgcgcgcaag
      181 ttcaacctgc cgctggtggc gacgaacgac gtgcattact gcttcgagga agacgccccg
      241 gcgcaggacg tcctcgtctg cgtgcagacg aatacgacga tcaacgacgc gaagcgcctg
      301 aagtcggaga cgaaccagct ctacctgaag agcccgcaac agatggagca gctcttcggc
      361 agcgtcccgg aagcgctctc gaacacgatc cgcatcgccg agatgtgcgc gctcgatctc
      421 ggcttcaagg ggtaccagct tcccgagttc gatgtcccag ccggtttcac cccggagacc
      481 tatcttcgca agctgtgcga agagggggcg atgaagcact acgggaccct tgatggcacg
      541 atcggggagc gtctctccta tgaactcggc gtcatcggtt cgatgggatt caccaactac
      601 ttcctcgtgg tgtgggactt tgtcctcttc gcgaagcaga acggcattct cgtcgggccg
      661 ggtcgcggtt cggcggcagg aagcctcgtc acgtactgcc tcgacatcac gggcctcgat
      721 ccgctcaagt acgatctcat cttcgagcgc ttcctcaatc cttcccgcat ctcgatgccg
      781 gatatcgata tcgacttcgc cgacgatcgc cgcgacgagg tcatcaagta cgtcgttcag
      841 aaatacggcg acgaccgcgt cgcccagatc gtcacattcg gaacgctgaa ggcgaaggcc
      901 gcggtccgcg acgttgggcg cgcgatgggc ctgagcttcg gagacaccga tcgcgtcgcg
      961 aagctgattc cgaccgatcc gaagatgacg atcaacctcg cgatgagtca ggttccggaa
     1021 ctctccaagc tgtatgacgg cgacggccag gtcaaggagt tgatcgacac cgcgctgaag
     1081 gtggaaggac tcgcccgcca ctcatccacg catgcggccg gcgtcgtgat ctcgcgcgat
     1141 ccgctggtgc agcatgtgcc gctccagcgc gcgggcggca agagcgaggg cgacgtcacg
     1201 acgcagtggg gacagggcca cctcgaggat ctcggtctgc tcaagatgga cttccttggg
     1261 ctgaagacgc tgaccgtgat cggcaaggcc gtcgacctgg cgcgggagag tggaagcgac
     1321 atatccatcg agacgatccc gatggacgat cagaaggcct tcgaactcct cggcaaggga
     1381 gagacggtcg gtgtcttcca gctggaaggc ggcatgacga cgcggatgac cgtcgacgtc
     1441 gcaccgtcga ccttcgagga catcatcgcg ttgatggcgc tgatccgtcc gggaccgatg
     1501 gaaatggcgc cggatttcat cgcgcgcaag cacggccggg ccgagatcgc atacgaccac
     1561 ccgatcctcg agaacattct caaggagacg tatggtgtcg ccctctacca ggagcaggtg
     1621 atgcagatcg ccaacgtcct ggctggcttc tcgatggccg aggcggacgg tctccgcaag
     1681 gcgatgggga agaagcagcc ggagatcatg gcgaagtacc gcgagcgatt catcgaggga
     1741 agcgtcgagc tcgggatcga gaagaagctc gccggcagcg tctacgacat gatcgagaag
     1801 ttcgccggct acggtttcaa caaggcccac agcgcgggct acgccgtcat cgccgcccag
     1861 acggcctacc tgaaggccaa ctatccggtg cagttcatgg cggcgctgat gtcgacggaa
     1921 atcgggaata ccgacaag
//
LOCUS       DASTMD010000284         4394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1363942, whole genome shotgun sequence.
ACCESSION   DASTMD010000284 DASTMD010000000
VERSION     DASTMD010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363942"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFQ54_04220"
     CDS             complement(<1..620)
                     /locus_tag="VFQ54_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164226.1"
                     /translation="MSKVQSNAPEGDNIESLQKQIMQGTLTRRQAMTHAAAAGLGAAA
                     ILALGGTPAFAQTPGASPAATPAGAAVTVALPDGTPDPSAKTGGRLKMGLQADPAGLD
                     PQITSLTAAWHVIEHVYDGLVAVGPSLEPEPALATSWEISADGLTYTFHLRKGVKFHN
                     GREFISKDVAYTFGRIVDPATASPNASDYTGIKSIATPDDYTAVFTL"
     gene            1873..3150
                     /locus_tag="VFQ54_04225"
     CDS             1873..3150
                     /locus_tag="VFQ54_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164227.1"
                     /translation="MNRRQLVKTAVFAGGVTLFSGFGPAPAHVGAQDKVKITQWYHQY
                     GEEGTQDAVGRYAQQFSASDAGKGIEVSVVWQSGTYGDVLSAALLTDSGPDVFEQNVP
                     TLDQVKQNQILALDDLYTPEVKADFNEVGLKAGTIDGKIYWVKMIDDTGAFYYKKSDF
                     AAAGITAVPTKLSEVLDVASKLNSGRKKGLFLGNDGGVGAAAGLLMFSGGGDFFNADE
                     TKVAFNTDGVAAGYTQLKALNDSGHLLLGAPADYWQPDAFLNGLAAVQWTGMWALPAV
                     VKALGDDVDIFPWPATDGTTGKQSTFWGGWGECVNGKTKYPDAAKALVKYLWIDSSDI
                     QKDWALSYGFHVPPRKSAAASAEQLKTGPAAKFVANLNSLGRVTPPLWDAAMGTAQTQ
                     AVTNIISNGADAKSELDAAAKTAQAELDRLLKG"
     gene            3262..4212
                     /locus_tag="VFQ54_04230"
     CDS             3262..4212
                     /locus_tag="VFQ54_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0164228.1"
                     /translation="MEVGIPTAVKTETPPWWRRTNGEATIAFVIFIAPMIIGLAVFTF
                     LPIGWGFLISFSEARNTISIGNWIGFDNYRAVMSDPEFRRSLRTIIIFAIFIVPITFF
                     LSLLLATLVPSIGFGKAFFRSVFFIPTAISYVVASVIWKQGLFSYSGFANVIVSWLDN
                     DNVLNWISPTGPAYYWVVLVSVRLWLQVGFYMIIFLAGLQEIDRSLYEAAYVDGGKPG
                     WKTFWTITFPLLRNTSIAVLLLNFIAAFQAYDEFVNILSSASGSSMLSLARPPLVYLR
                     QVALDQQDYGRGSAGAFILTAIIIAVTLIQGRLFGFGRRS"
BASE COUNT          806 a         1450 c         1324 g          814 t
ORIGIN      
        1 agcgtgaaca ccgccgtgta gtcgtccggc gtcgcgatcg acttgatgcc ggtgtagtcg
       61 gatgcattcg gggatgccgt cgccggatcg acgatgcggc cgaaagtgta ggcaacgtcc
      121 ttcgagatga actcgcggcc gttgtggaac ttcacgccct tgcggaggtg gaaggtgtag
      181 gtcaggccgt cggcggaaat ctcccacgac gtcgccaggg caggctccgg ctcgagggac
      241 ggaccgacgg cgacgaggcc atcgtagaca tgctcgatga cgtgccacgc cgcggtcagg
      301 ctggtgatct gcgggtcgag accggccgga tcggcctgga gacccatctt gagacgaccg
      361 cccgtcttcg cactcggatc cggcgtgccg tccggcagcg caacggtcac ggcggcgccg
      421 gccggggtcg ccgcgggcga cgcaccaggc gtctgcgcga atgccggagt tccacccagt
      481 gcgaggatcg ccgcggcgcc gagaccggcg gcagcggcat gcgtcatcgc ctggcggcgg
      541 gtcaacgttc cctgcatgat ctgcttctgc aacgactcga tgttgtcgcc ttccggcgcg
      601 ttcgactgca ccttgctcat tccttgctct ctcctgtgcc tgcggcgatc gcccaggcat
      661 atgaaaacgg gatcgccgcg caatgcggtc gatccttggc cccaagaagg tgatgcacgt
      721 cggaggacgg aactcacccg caccggactc gccacgattt ccgccttctc gcgcgaagga
      781 aggaccgcga cgtttccgca ctcgtggcga cgatgatagt ggcgacgcca ttcagacccg
      841 gaacgaggat ggcagagtcg agacgatcct catctcacac catatacgcg tatcgagtat
      901 acgagccgtc ttctcaccta cacaacgaag cgtgtcactt tccgccaatc ccggttgcat
      961 cgcgattcca gccgtgggcg caatgcacga atatcccgta tcgcgtcgta cgttccgacg
     1021 ccgcgtgtgc cagatacgcg tattgtggct ggggttcccc tgtaacgttg tcgcatgaac
     1081 cgaacggggc ggagaacggg tctcggtcgg cgctccctgg cgcggagcgt tgttctgccg
     1141 aatccgcgtc gcggcggggt ataaaacccc gccctaccaa cccgacagca tcaaacgagt
     1201 cggcagttcc ggattccgct atgagggggg acgatctccg caccccggga cgcggctcgg
     1261 gaccatccgt cgccaacggg gaggcgggac attcctccat ccagcgaccg aagatcgtgc
     1321 tgccagcgat cctgaggcag gtcgctgcac tccgtagtcc aaagaccggc attcaggggg
     1381 tctttggagc aaggcgcgcg gtctgtggat ggaggaatgt cccgcctggt gctggcccgc
     1441 ggcccgttcc cggcagacgg atcggcgacg ccgatccacg tcgtggccgg cgatcgacat
     1501 gccggcgccg agcattaccg gcgtcccatc tcgaaactcc cggacagccc catccgcaag
     1561 agtcgaccgt ccgaaaccgc aaatagcggg caatcgcgcg acgctacgcc gacgttttcg
     1621 ccacgatacg ttgaacgcgg atggaagcga tgggccggat ccccaacttg aggattgccg
     1681 gcgccgttcc tgccccatac gcggattgtg gttgacattt attcgtcgtc ggcataaagt
     1741 ccactcaaac cacaacccaa tccgtgacga gggtctttac agttgcgccg agcagctaga
     1801 aagcgtgtgc acatggatcg taagccaacg aaggcttcat cttcgagcag cccctctctt
     1861 tcgacgtttg gcatgaaccg ccggcaactc gtgaagactg cggtcttcgc tggtggtgtc
     1921 acgctcttct ccggattcgg tccggcgccc gctcatgtcg gcgcgcagga caaggtcaag
     1981 atcacgcagt ggtatcacca gtacggtgaa gagggcaccc aggatgccgt tggccgatac
     2041 gcgcagcagt tctccgcgtc ggatgccggc aagggaattg aagtctccgt cgtctggcaa
     2101 tctggcacgt acggcgatgt cctcagtgcc gcgctcctca ccgacagcgg ccccgacgtc
     2161 ttcgagcaga acgttcctac cctcgaccag gtaaagcaga accagattct cgcgctggac
     2221 gatctctaca ccccggaggt caaggcggac ttcaacgagg tgggtctcaa ggccggcacg
     2281 atcgacggca agatctactg ggtcaagatg atcgacgaca ccggcgcgtt ctactacaag
     2341 aagagcgatt tcgcagccgc cgggatcacc gccgtcccga cgaagctcag cgaggtgctc
     2401 gacgtcgcga gcaagctcaa ctccggacgc aagaagggcc tcttcctcgg caacgacggt
     2461 ggcgtcggcg ccgcggcggg tctgctcatg ttctcgggcg gtggtgattt cttcaacgct
     2521 gacgagacaa aggtcgcctt caataccgac ggcgtcgccg caggctacac ccagctcaag
     2581 gcactgaacg attccgggca tctcctcctc ggcgccccgg cggattactg gcagcccgac
     2641 gccttcctca acggcctcgc cgccgtccag tggaccggca tgtgggcact tcctgcagtc
     2701 gtcaaggcgc tcggcgacga cgtcgacatc ttcccctggc cggccacgga tggtacgacc
     2761 ggcaagcagt cgaccttctg gggcggctgg ggtgaatgcg tcaacggcaa gacgaagtac
     2821 cccgatgccg cgaaggcgct cgtcaagtac ctctggatcg acagctccga tatccagaag
     2881 gactgggcac tctcctacgg cttccacgtc ccgcctcgca agagcgcggc agcctctgcc
     2941 gagcagctca agaccggacc tgccgccaag ttcgtcgcca atctcaacag cctcggccgg
     3001 gtaacgcctc cgctgtggga cgccgcgatg ggcacggcgc agacgcaggc cgtgaccaac
     3061 atcatctcca acggcgccga cgcgaagtcc gaactcgacg cggcagcgaa gacggcccag
     3121 gccgagctcg atcgcttgct gaagggctga tcctcacccc atggagtgat cagatagagg
     3181 gtggcgcagg ttgccaccct ctattccaat tcgccgcaca ccgagcgttc tgtgcggtac
     3241 gtcgctacgt gggagggtca tatggaagtc ggcatcccca ccgcggtcaa gaccgaaacg
     3301 ccaccgtggt ggcgtcgtac caacggtgaa gccacgatcg ccttcgtcat cttcatcgca
     3361 ccgatgatca tcggtctggc ggtcttcacc ttcctgccaa tcgggtgggg tttcctgatc
     3421 agtttctccg aggcgaggaa cacgatcagc atcggaaact ggatcggatt cgacaactac
     3481 cgcgccgtca tgagcgatcc cgaattccgg cgctcgcttc gcacgatcat catcttcgcg
     3541 atcttcatcg tgccgatcac cttctttctc tcgctcctcc tcgcgacact cgtgccctcc
     3601 atcggcttcg ggaaggcctt cttccgcagc gtcttcttca ttccaaccgc aatctcctac
     3661 gtcgtcgcgt cggtgatctg gaagcagggg ctcttctcgt attccgggtt cgcgaacgtc
     3721 atcgtttcgt ggctcgacaa cgacaacgtc ctcaactgga tcagtccgac cggccccgcc
     3781 tactactggg tcgtcctcgt ctccgtgcga ctgtggctgc aggtcggctt ctacatgatc
     3841 atcttcctgg ccgggctgca ggaaatcgac cgctccctct acgaggccgc ctatgtcgat
     3901 ggcggaaaac cgggctggaa gaccttctgg acgatcacct tcccgctcct ccggaacaca
     3961 tcgatcgccg tcctcctgct gaatttcatc gccgcattcc aggcctacga cgaattcgtc
     4021 aacatcctga gcagcgcaag cggcagttcg atgctgagcc tcgcaaggcc accgctcgtc
     4081 tacctgcgcc aggtcgcgct cgatcagcag gactacggcc gtggctctgc gggcgccttc
     4141 atcctcaccg ccatcatcat cgccgtgacg ctgatccagg ggcgactctt cggattcggt
     4201 cgccggtcct gaccccgcct cacctacgtc gagttacgga gaacgaagga gcgcgcatca
     4261 tgagtgtgca gacgaccagc aagccggatc gatggggagg gctcgatcga cctcgcggat
     4321 cgcgcgccgg cgggttcgcc atcgcctcag cgatcggccg gtacgggttg ctgctgatcc
     4381 tgtcggcgat cttc
//
LOCUS       DASTMD010000285         2783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_293464, whole genome shotgun sequence.
ACCESSION   DASTMD010000285 DASTMD010000000
VERSION     DASTMD010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293464"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VFQ54_04235"
     CDS             complement(<1..499)
                     /locus_tag="VFQ54_04235"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEU0164229.1"
                     /translation="MAGPIYRSSTTAPAGFVRFVGSLAISFAGMLVLPLGASAHTSQA
                     VAPRDLMQFWTPDPTVIVACLVTGWLYLRGVGRLREIGERTGRGPIIQTGRIAAFWCG
                     LATLLIALASPLDTATASVFSAHMVQHVLLIAVAPPLILLGNPMTVIMNGLPRSWRRP
                     IAQLEH"
     gene            1017..2321
                     /locus_tag="VFQ54_04240"
     CDS             1017..2321
                     /locus_tag="VFQ54_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU0164230.1"
                     /translation="MTILDATSIARPTPSQLAYQRERSFALFFHFGINTFYGKEWSDG
                     TLDPAGFDPTDLDSRQWVDIAKRAGAAHVILTAKHHDGFCLWPSGTTDYSVKSSPWRG
                     GRGDVVGELADACRDAGIGLGLYLSPWDRHERSWAEDQPAYDRFYLRQLEELMTNYGP
                     LVEVWFDGAGSEQHPYDWGAITAVVDRHQPDALVFNMGRPAIRWVGNEDGLAADPCWY
                     TVDETAKSAYTDDREGLPGGGWYLPPECDVAIRRHWFWQDDDLQTLKSINHLEAIWYR
                     SVGLGANLLLNVPPDRRGLIDDRDAERLLEFAGTIRDRFANPVPAMLVQDGRSIVAIF
                     PEATTFDHLILREAIEDGQRIGSHRIVGTATGAGIVTGVFTVGSQRVHAFPAVTTASV
                     TIEIDDPLGQLVSVDAFHTGVEALPALEDQPRFMSEKVDPKI"
     gene            2376..>2783
                     /locus_tag="VFQ54_04245"
     CDS             2376..>2783
                     /locus_tag="VFQ54_04245"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEU0164231.1"
                     /translation="MAFNGLGMHLGNLSQLSDARTRSISPENFTGAKGQGGMATEGTG
                     AVPGRELGQGWKISPSIVIAPHELVTLAEIEGPGAIQHIWLTVHPTWWRRLVLRIYWD
                     GEETPSVETPLGDFFANGWCQRCNISSLPIAVNP"
BASE COUNT          539 a          832 c          918 g          494 t
ORIGIN      
        1 ggtgttcgag ctgggcgatc gggcggcgcc acgagcgggg gaggccgttc atgatcaccg
       61 tcatcggatt gccgagcagg atgagcggcg gcgccacggc gatcagcagg acgtgctgaa
      121 ccatgtgggc ggaaaagacc gacgcggtcg ccgtatcgag tggtgaggcg agggcaatga
      181 gcaacgttgc taatccacac cagaacgcgg caatgcgtcc tgtctgaatg atcggaccac
      241 gtccggttcg ttcgccgatc tcgcggagtc tgccgacgcc gcggagatag agccatccgg
      301 tcaccagaca ggcaacaatg actgtcggat cgggagtcca gaactgcatg agatcgcgtg
      361 gggcgaccgc ctgtgacgta tgcgcggagg cgcccaacgg cagaaccagc atccctgcga
      421 aagaaatcgc aagggagccg acaaaacgca cgaaacccgc tggcgcggtg gttgaactgc
      481 gatagattgg accggccatg gtcgcctcaa tcgaaacgaa tcgcgcgtgc cgaagtgaaa
      541 attgtaacgg gtttccattc agcctgaaga cttcaccgat ccgccccacg tgcggggaag
      601 gtgcatggat gatgctgaca cgtcgatggg tcaactctca agggacgtcg ggaatcagtg
      661 cccacgagag gatcgggccg aggattcgca ggtcgtttcg cgctccgaac tcctagacat
      721 ctgttgaata acttgcgaaa tatcgagata acgcgcggcg ccccaactcg tggtccgggc
      781 acaagttcag acccggattt ccgtcgaaac tgtcgtggta acgttcgcat cttcctatct
      841 atgtagggat cagttcgcga ctttcccgat ctcctgtcag ggattccccc acccgacgga
      901 tggacgacgc gatccctggt cgcgatcgca tctttcggcg gcggacgtac ccacgttcgg
      961 tggtctcatg gttgaatgtc ccggacagga acattcgaat gtcatgggaa atgggggtga
     1021 ccatcctgga cgccacatcg atcgcacgac caacgccgtc gcagctcgcc taccagcggg
     1081 aacggtcgtt tgcgctcttc tttcatttcg ggatcaacac gttctacggc aaggaatgga
     1141 gcgatggaac gctcgatccc gcgggattcg atccgaccga cctcgatagc cggcagtggg
     1201 tcgacatcgc gaagcgcgcc ggcgcggcgc acgtcatcct gaccgcgaag caccatgacg
     1261 gtttctgcct ctggccaagc gggacgacgg actattccgt gaagtcgtcg ccgtggcgag
     1321 gcggcagggg agacgtcgtc ggcgagctgg cggacgcctg tcgcgacgcg gggatcggtc
     1381 tcgggctcta tctgtcgccg tgggatcgtc acgagcgatc ctgggccgaa gaccaaccag
     1441 cctacgacag gttctatctg cgccagctcg aggagctgat gacgaactat ggaccgctgg
     1501 tcgaggtctg gttcgacggg gcaggatccg agcagcaccc atacgactgg ggcgcgatca
     1561 cggcggtcgt cgatcggcat cagccggatg cgctcgtctt caatatgggt cgcccagcga
     1621 tccgttgggt cggcaacgag gatggtcttg ccgccgaccc gtgctggtac accgtcgatg
     1681 agactgcgaa gtcagcctat accgacgacc gcgaggggct cccgggtgga ggctggtacc
     1741 tgcctccaga gtgcgacgtc gcgatccgtc ggcactggtt ctggcaggat gatgacctgc
     1801 agacgctcaa gagcatcaac catctcgagg cgatctggta ccgctcggtc gggcttggcg
     1861 cgaatctgct gctgaacgtc ccgcccgacc ggcgcggcct gatcgacgat cgggatgcgg
     1921 agcgtctcct cgagtttgcc gggacgattc gggaccgctt tgccaacccg gttccggcga
     1981 tgctcgtgca ggatggacgc tcgatcgtcg cgatcttccc cgaggcaaca accttcgatc
     2041 acctcatctt gcgggaggcg atcgaggatg ggcagcggat cggttcgcat cggatcgtcg
     2101 ggactgcgac gggggccggg atcgtgaccg gtgttttcac ggttggcagc caacgcgtgc
     2161 acgcctttcc ggcggtgacg acggcgagcg tgacgatcga gatcgacgat ccgctcggcc
     2221 agcttgtcag tgtcgacgca tttcacacgg gggtggaggc cctccccgcg ctcgaggatc
     2281 agccgcggtt catgagcgag aaggtcgacc cgaagatata gaccacattc aatctgacgt
     2341 cagtcgcgac agattcaccc cggagggaga agacgatggc attcaacgga ctcggcatgc
     2401 accttggtaa cctcagccag ctctcggacg cgcggacgcg gtcgatcagc ccggagaact
     2461 tcacgggcgc caagggccag ggcgggatgg cgaccgaggg caccggcgcg gttcccggtc
     2521 gcgaactcgg gcagggctgg aagatttcgc ccagcatcgt gatcgcaccg cacgaactcg
     2581 tcacgctcgc cgagatcgag ggaccggggg cgatccagca tatctggctc accgttcacc
     2641 cgacctggtg gcgacgcctt gtgctccgca tctactggga tggggaagag acgccgtcgg
     2701 tggagacgcc gctcggcgac ttcttcgcga acggctggtg ccagcgctgc aacatctcgt
     2761 cactgccgat cgcggtgaat ccg
//
LOCUS       DASTMD010000286         1573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_25404, whole genome shotgun sequence.
ACCESSION   DASTMD010000286 DASTMD010000000
VERSION     DASTMD010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25404"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1573
                     /locus_tag="VFQ54_04250"
     CDS             <1..>1573
                     /locus_tag="VFQ54_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164232.1"
                     /translation="TSLQGRIPASQEVVADAIQIEGVTYSALVHDWIEGPTLAAAVGR
                     ARKAGNGGVLRALAKAVLDLANSLRAAGFAHQNLNADNLIISPTGSLMAVGLDAAKWP
                     QSPPGATPHRTPPFLHPKGAANPFEQDAFATLLIYATLRAMADDPALAATYGDPIGTP
                     GGALLFSTWDLADPSRSRAFGEIAANVRSDTRELIDALRRACTAPSAEVERWAQAIPG
                     IVALPASVQAREEPTAVLPAAPANESWDVSDAIERIKARFQGEEEEAPRAPAADPAPS
                     SWNSWGVPVQPQTPPNPPPPPAARETRARAQSPQVAPSGWETRREPIAAPRVARVPPP
                     SVAPEPADDGWTTVAPAAIVNGQIVTAEPEPATPAPERRPRRRVRTVEVPAETWEGIV
                     PEPVLMTPVSLIEDRRALRAALTRRDAAVAFQLWERLADDPVGRTFALEVERLRSVQI
                     RTRIREEAGRKDDREVIRLAESARIDGSVLDADTRMIVRSARDRAEVGDRLERALAGS
                     DRDRLADLAVTGDLMA"
BASE COUNT          240 a          547 c          552 g          234 t
ORIGIN      
        1 tcacctccct gcaaggccgg attccggcgt cgcaggaggt cgtcgcggac gcgatccaga
       61 tcgagggcgt cacctattcg gcacttgtgc atgactggat cgaaggacca acgctcgccg
      121 cggcggtcgg acgggcgcgg aaggcgggca atggtggcgt gctccgggcg ctggcgaagg
      181 ccgtgctcga tctcgcgaac tcccttcgcg cggcaggatt cgcgcatcag aacctcaatg
      241 ccgacaacct catcatctcc cccaccggtt cgctgatggc ggtcgggctc gatgctgcga
      301 agtggccgca gtcgcctccg ggcgcgacgc cgcaccggac accacccttc ctccatccga
      361 agggcgcggc gaacccgttc gaacaggacg cgttcgcgac gctgctcatc tatgccacgc
      421 tgcgggcgat ggcggatgac cccgccctcg cggcgacgta tggcgatccg atcgggacgc
      481 ccggcggggc gttgctcttc tccacctggg atctcgccga cccgtcgcga tcgcgggcgt
      541 tcggcgagat cgccgcgaat gtgcgctccg acacgcggga actgatcgac gcgttgcgcc
      601 gggcctgtac cgcaccatcg gccgaggtcg agcggtgggc ccaggcgatt ccggggatcg
      661 tcgcccttcc cgcgtcggtt caggcgcgtg aggagccgac agcggtgctc ccggcggctc
      721 cggcgaacga atcgtgggat gtgtcggacg ccatcgagcg gatcaaggcg cgtttccagg
      781 gcgaggagga agaggctcct cgcgccccgg cggccgatcc cgctccatcc tcgtggaact
      841 cgtggggcgt gccggtgcag ccgcaaaccc cgcccaaccc gccacccccg ccggcggcgc
      901 gggaaacgcg agcgcgagcg caatctcccc aggtcgcccc ctcgggatgg gaaacacgtc
      961 gtgaacccat cgctgctccg cgtgtcgccc gggttccgcc tccatccgtt gccccagagc
     1021 cggccgatga tggctggaca acggtggccc cggcggcgat cgtcaatggc cagatcgtga
     1081 ccgccgaacc ggagccagcg accccggccc cggagcgacg gccccggcgt cgcgtacgga
     1141 cggtcgaggt tccagccgag acctgggagg gaatcgtccc tgagcccgtg ctgatgacgc
     1201 ccgtctcatt gatcgaggat cgccgcgctc tgcgggcggc cctgacaaga cgggatgccg
     1261 ccgtcgcatt ccagctctgg gagcgattgg cggacgatcc cgtcgggcgg acattcgcgc
     1321 tcgaggtcga acggctgcga tcagtgcaga tccggacccg aatccgtgag gaagcggggc
     1381 ggaaggacga tcgcgaggtc attcggcttg ccgagtcggc gcggatcgac ggatccgtgc
     1441 tcgatgccga tacacggatg atcgtgcgaa gtgcgcgtga ccgggcggag gtcggcgatc
     1501 ggctcgaacg tgccctcgcc ggctccgatc gcgatcgcct cgccgacctc gcggtgaccg
     1561 gcgacctgat ggc
//
LOCUS       DASTMD010000287         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1722681, whole genome shotgun sequence.
ACCESSION   DASTMD010000287 DASTMD010000000
VERSION     DASTMD010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722681"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1106)
                     /gene="thiL"
                     /locus_tag="VFQ54_04255"
     CDS             complement(81..1106)
                     /gene="thiL"
                     /locus_tag="VFQ54_04255"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEU0164233.1"
                     /translation="MPDDRVSDIGEFELIERLRAAIPESVRTTGQVSTGIGDDAAIWM
                     PTPGLASVITTDTLVEGVHFRLDWTGWISLGFKALAVNLSYIAAMGAIPRLATVSLGL
                     RGTERVANLVALYQGIGRLAETHGVALVGGDIVHDPDHVTISVTAIGEVEPERVLRRS
                     GALAGDLICVSGSIGASAAGFEILRDPVPYIGLATTGLLKAAHERPNPRVALGRLLGE
                     IGATAAMDLSDGLLGDLPKILAASGVAATIDSRSLPVAPAVKALFPDLWIDLALRGGE
                     DYELLFTIPPGLFPALAARVEEIGATVTAIGTIEERADDTSGITLIGPDGNSAPVAPG
                     VSWDHFT"
     gene            complement(1099..>2275)
                     /locus_tag="VFQ54_04260"
     CDS             complement(1099..>2275)
                     /locus_tag="VFQ54_04260"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU0164234.1"
                     /translation="RCSRERPVARRLRYTRCRIGRAGGKRNGGEMRIGINAALTGAGE
                     GYRQTGISRYIGELVSSLRPQLAVDDDLVLFGNDSRLISERPGARIAWEQTVLPAAAI
                     AKGVDVLHGPLSVVPAIPGFRKVVTVHDLAFVRYPEQIARSRRAYLVAGTRLGARIAD
                     RIIAVSTKTADDLMAWLDVPADRIMVIPLAPSPKVSRLIGDALERFKVTMGLQRPYLL
                     AVGTLEPRKNLPFLLDAFAAVKDTIPHDLVLVGPEGWLNGPFRERLERLKLGDRVRLT
                     GFVSDEDLGAWYSGADLFAFPSIYEGFGLPTVEAMSCGAPVLTSSSSCFPEVVGDAGV
                     MVAPDDRAGWSEAIVRILRDAVLRDDLRARGLARAAGFSWERTAAQTYDIYRTVANA"
BASE COUNT          430 a          797 c          703 g          345 t
ORIGIN      
        1 cgaaggataa caagactcat tgtcacaatg accgcagaat cacaaggaac gcgggacgcg
       61 accactgtat cgcgccaggc tcacgtgaag tggtcccagg agaccccggg agcgacaggt
      121 gccgagttcc cgtctggccc gatcagtgtg atcccgctcg tgtcgtccgc ccgctcctcg
      181 atcgttccga tcgccgtcac cgtggcgcct atctcctcga cccgggcagc gagggcaggg
      241 aagagaccgg gggggatcgt gaagagcagc tcgtagtcct ctccgccgcg aagggcgaga
      301 tcgatccaca gatccgggaa gagcgccttc acggccggag caacgggaag cgaacgggag
      361 tcgatcgtgg ccgctacccc gctggccgcg aggatctttg ggagatcgcc gagcaggccg
      421 tcggagagat ccatcgccgc cgttgccccg atctcgccca ggagccggcc gagcgcaacg
      481 cgtggattcg gtcgctcgtg cgccgccttg agcagcccgg tcgtcgccag accgatgtat
      541 gggactggat cgcggaggat ctcgaatccg gcggccgagg cgccgatcga gccgctgaca
      601 cagatcagat ctcccgccag cgccccactc cgccgcagga cgcgctccgg ttcgacctca
      661 ccgatcgccg tcaccgagat cgtcacgtgg tcggggtcgt gaacgatatc gccgccgacc
      721 agcgccaccc cgtgcgtctc cgcgaggcga cctattccct ggtagagggc gaccaggttg
      781 gcgacgcgct cggtcccgcg aagaccgagc gacacggttg ccagccgcgg aatcgccccc
      841 atcgccgcga tgtaggagag attcaccgcc agcgccttga atccaagact gatccagccg
      901 gtccagtcga gccgaaagtg aacgccctcg accagcgtgt cggtggtgat gaccgaggcg
      961 agccccgggg tcggcatcca aatcgcggca tcgtcgccga taccggtgga aacctggccc
     1021 gtggtccgca cgctctcggg gatcgctgct cgcaggcgct cgatcaattc aaactcgccg
     1081 atatcggaga cacgatcgtc aggcattggc caccgttctg tagatgtcgt aggtctgggc
     1141 cgccgtgcgt tcccatgaga acccggcggc acgcgcaaga ccgcgtgccc gtagatcgtc
     1201 tcgcaacacc gcgtcccgga gaatccgcac gatcgcctcg gaccagcccg cgcgatcgtc
     1261 cggcgcaacc atcacaccgg catcgccgac aacttccggg aaacaggagg aactggacgt
     1321 caacaccggc gcgccacagg acatcgcctc caccgtcggc aacccgaatc cttcatagat
     1381 ggagggaaac gcgaagaggt ccgccccgct gtaccaggcg cccaggtcct catcgctgac
     1441 gaagccggtc aagcgaaccc gatcgcccag cttcaatcgt tccagtctct cccgaaacgg
     1501 cccattgagc catccctccg gtccgacaag cacgagatcg tgcgggatcg tgtctttcac
     1561 cgccgcgaag gcatcgagca ggaagggcag gttcttgcgg ggctccagcg ttcccacagc
     1621 gaggagatag gggcgttgaa gccccatcgt caccttgaac cgttcgagcg cgtctccgat
     1681 caaacgtgac accttcggcg acggcgccag cggaatcacc atgatccgat cggctggcac
     1741 gtccagccag gccatcaggt cgtccgccgt cttcgtcgag accgcgatga tccgatccgc
     1801 gatccgcgcc ccgagtctcg ttcccgcgac gagataggcg cgccgcgacc gggcaatctg
     1861 ctccggatat cgcacgaagg cgagatcgtg cacggtgacg accttgcgga aaccgggaat
     1921 cgcgggaacc acactcaacg gcccgtgcag cacgtcgacc cccttcgcga tcgcggccgc
     1981 cggcagaacc gtctgttccc acgcgatccg cgcgcccggt cgttcggaaa tcagccgcga
     2041 gtcgtttccg aaaagcacca ggtcgtcatc aacggcgagt tgcgggcgca gggatgagac
     2101 cagctcgccg atatagcggc tgatacccgt ctgacgatac ccctcgcccg cgcccgtcag
     2161 cgccgcgttg atcccgattc gcatctctcc gccgttccgt ttgccgccgg cgcggccaat
     2221 cctgcaccga gtgtaccgaa gccgcctcgc caccggacgc tcacgggagc atcgg
//
LOCUS       DASTMD010000288         1747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_114258, whole genome shotgun sequence.
ACCESSION   DASTMD010000288 DASTMD010000000
VERSION     DASTMD010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114258"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /gene="lgt"
                     /locus_tag="VFQ54_04265"
     CDS             <1..688
                     /gene="lgt"
                     /locus_tag="VFQ54_04265"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU0164235.1"
                     /translation="VVVIGIVGARTYYVLLKFDFYIHHPEEAINIRLGGLTIHGAIVG
                     GVATMLILCRRNREPFFRWADVMVPGLALGQALGRWGNWANQEAFGTPTTLPWAVHID
                     PDRRPPEYAQYSTFHPTFLYESLFDLINALVLAWLVLRMPRIRYLKAGDVAWIYLVAY
                     GVVRFIIESDRTDSLYIGPLPAAYWLSFALIGVGVVMFVVRRTWLNDLEDDHPERYEA
                     AQEVELVPGR"
     gene            980..>1747
                     /gene="hflX"
                     /locus_tag="VFQ54_04270"
     CDS             980..>1747
                     /gene="hflX"
                     /locus_tag="VFQ54_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU0164236.1"
                     /translation="MTAFEEQTSERLRNTQAAPEQALLVAVELPNQTWDVQDELEELA
                     SLAETVGVEIVGSVTQKLDHPHPSTYVGKGKLAEIKELKETLGYDFVLCDGELTPAQL
                     RNMEKALDVKIIDRTALILDVFASRAQTHEGRLQVELAQLQYRLTRLTRMWTHLSRQT
                     GGGGVGLRGTGETQLEADRRESQKRITFIKDQLEQVHKSRELYRDRRRTTPISVVSLV
                     GYTNAGKSTLMNTITGAGVLAEDKLFATLDPTTRRIAL"
BASE COUNT          339 a          551 c          546 g          311 t
ORIGIN      
        1 ggtggtggtg atcggtatcg tcggcgcccg cacctactac gtgctgctca agttcgattt
       61 ctacatccat caccccgaag aggcgatcaa tatccggctc ggcggcctca cgatccacgg
      121 ggcgatcgtt ggtggcgtgg cgacgatgct gatcctctgc aggcgaaacc gggagccctt
      181 cttccgttgg gccgacgtga tggtgcctgg gctggcgctc ggccaggcgc ttggtcgctg
      241 gggaaactgg gcgaatcagg aggcgttcgg caccccgacg acgctcccgt gggccgtcca
      301 catcgatccc gaccgtcgtc cacccgaata cgcgcagtac tcgacgttcc acccgacgtt
      361 cctctacgaa tcgctcttcg atctgatcaa cgcgctcgtg ctcgcctggc tcgtcctgcg
      421 gatgccgcgg atacggtacc tgaaggcggg agacgtcgcc tggatctacc tcgtcgccta
      481 tggcgtcgtg cgtttcatca tcgagagcga ccggacggat agcctctaca tcggcccgtt
      541 gccggcggcg tactggctga gcttcgccct gatcggagtc ggcgtcgtga tgttcgtggt
      601 ccggcgaaca tggctgaacg atctcgaaga cgatcatccg gaacgatacg aagcggccca
      661 ggaagtcgag cttgtcccgg gacgataatg cgcgaaggta cttgtgcggg cagggtatga
      721 ttaggggaag cttcaattcc gcaagcgatc gtgaacctgc gcgatccgca ggtgttgggg
      781 ctctgtgacg ccgctttggc gggggatggg ccagtgacag gacgcacgtc cgtcaccaat
      841 taagagagaa cgaggtggtc tgccatgggc atcgtgcgaa catggatcga tcccaatccg
      901 attccaaccg cgcaaatgca ccgtaatctg tttcagtaag cccctcaccg atgcggtgag
      961 ggcatagagg agactcacca taaccgcatt cgaagaacag acctcagaac gactccgcaa
     1021 cacgcaggcc gcgccggaac aggcgcttct cgtcgccgtg gagcttccca accagacctg
     1081 ggatgtccag gacgaactcg aagaactcgc aagcctcgcc gagaccgtcg gtgtcgagat
     1141 cgtgggatcg gtcacgcaga agctcgacca tccgcatccc tccacctacg tcggcaaggg
     1201 caagctggcc gagatcaagg agctcaagga gacccttggc tatgatttcg tgctctgcga
     1261 cggggaactg acgcccgccc agctccgcaa tatggagaag gcgctcgacg tcaagatcat
     1321 cgaccgcacg gcgctcatcc tcgatgtctt cgcgagccgc gcgcagacgc acgaggggcg
     1381 tctccaggtc gaactcgccc agctgcagta ccgcctgacc cgcctcaccc ggatgtggac
     1441 gcacctttcg cgtcagaccg gtggtggtgg cgtcggcttg cgtggcaccg gtgagacgca
     1501 gctcgaggct gaccgccgtg aatcgcagaa gcggatcacc ttcatcaagg atcagctcga
     1561 gcaggttcac aagagccgcg agctctatcg cgatcgccgg cggacgacgc cgatcagcgt
     1621 cgtgtcgctc gttggctaca ccaacgccgg caagagcaca ctgatgaaca cgatcaccgg
     1681 cgcgggcgtg ctcgccgagg acaagctctt cgcgacgctc gacccgacga cgcggcgcat
     1741 cgcgctg
//
LOCUS       DASTMD010000289         5526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_651401, whole genome shotgun sequence.
ACCESSION   DASTMD010000289 DASTMD010000000
VERSION     DASTMD010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651401"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1166
                     /locus_tag="VFQ54_04275"
     CDS             180..1166
                     /locus_tag="VFQ54_04275"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEU0164237.1"
                     /translation="MQNRPDNGGEIDLERSRERDPEFDVADSLTEPLPTSADGPLAVT
                     THQGGRFWPGRESTWYWPFLGLIGVLADLLFSFLANVIPFNGQTTDDILTKTKMPFQP
                     SAWVWAIWVAIFILMAVYVVYMIFPGGRHSDRTRRIGPFFLIVNVANVLWLFFWHWNQ
                     FLLSLILMAIVLASLAIIYLMANSKDDEGNKPTRTQLLMVRIPFSVYLGWTTVATLGN
                     LMVWLQHRGWEGAPFSLRVWAVIFMIVGLLAAAAFAAFRQDAAYAIVFVWAYVAVAQH
                     QWDISKLVSIFAGIGAVVAAGLAVMAFMLSFDRRAMSGMYVEEHRENPLPLE"
     gene            1437..2702
                     /locus_tag="VFQ54_04280"
     CDS             1437..2702
                     /locus_tag="VFQ54_04280"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164238.1"
                     /translation="MRAPEALENPNFRRYWSSQAVALAGTWMQNTSASLVVLSLTSSA
                     LLIGAINVISALPLLLFSLFGGVVADRFDRRKILMTTQSLLGLISLVYASLIFSDKIE
                     FWHILVIAAVSGSVAAFDLPASQAFVSELVKREDLPQALALNSASFNATRTIGPALAG
                     VVIGLFGTAWAFVLNSVTLLAPISTLYGLRKVMTKPIRAARASGVTQLRAGVAHIRKS
                     EELTGLILLTTIFSFFVFPNLLVLMPLYITDVLGGSDTWVAIMISVLGIGSLIGSIAM
                     IRGSRLEVAAGRRLRTSMVGLTVGLTWLALSPNPWVAIPGVMISGYSFATGNTQISTR
                     VQQLAPDELRGRVLSVNGLAFNGVMPFATIAVSGLSEVVGQHVVMGGCAICLAVGSFL
                     IWKRYAWKAFLPNSTPIHVASATPVIAGH"
     gene            3443..4840
                     /locus_tag="VFQ54_04285"
     CDS             3443..4840
                     /locus_tag="VFQ54_04285"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164239.1"
                     /translation="MTDEPPPVEGRVPKGVMPRSLRLAKPWNRSAPPRTAPVVGHPDE
                     IAPHSPRLALRDLQVPKALQHPPFRRFWSGQLVALIGMWGQSTASNLVFLTLTSSAFL
                     IGLINIVSAVPMLLLSLFGGVIADRFERRKILTMTQTVIASLSVVWAILIFTDRIAYW
                     HVLVIAAVGGVVVSFDLPAGQAFLSQIVRREDMTEAIALNSASINATRIFGPILAGIL
                     IGVVGTGVAFVAHSTAVMIFVITIFSLRAMVPHSRPVGSRVPGITALRDGLRHIRHSD
                     ELIGLVGVTAIFSFLAVPPLLVLMSLYVTKTLGGSDAWVPTMTSIFGAGSLIAAIALF
                     RGSRLEAAAGRRMRLTMAGLAVGLMWLALAPNPWLAIPGILIAGCSFEMGLIQVQTRV
                     QALAPDDLRGRVLSVNGLAFNGVMPFSTLTISTATTELGAPLVLGACSVLVAICSVLI
                     WKRYTWKAFVPAPAI"
     gene            complement(5152..>5526)
                     /locus_tag="VFQ54_04290"
     CDS             complement(5152..>5526)
                     /locus_tag="VFQ54_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164240.1"
                     /translation="CYGDVEESHFIQPKDAGLLAAVLTGIFENATRPITWVHLPVPIE
                     RDDEAFFAPLANVPWPADTEVYLGLIHHEDGVEGAERRAKAAEKFVPNFGVATECGFG
                     RGPRERTPGLLDLHAAVAAPVR"
BASE COUNT          827 a         1837 c         1789 g         1073 t
ORIGIN      
        1 cgccgcccca acctccatca cgacgcgcct cgcggatgat cggtgtcgtc aagggaggaa
       61 cccggtgata ccggcgtgtt gaacggccat tcgtgtccgg atcgtttctt gtctccactg
      121 ctggcacaat aggctgaaca cttgtggtga cgggacgcgt tgaaggaaat ggacgactga
      181 tgcagaaccg acccgacaac ggcggggaaa tcgacctcga gcgcagccgg gagcgagatc
      241 cggagttcga cgtcgcagac tcgctgaccg agcccctgcc gacttccgcc gatggcccac
      301 tcgcggtcac gactcatcag ggaggccgct tctggccggg gcgcgagagc acctggtact
      361 ggccgttcct cgggctgatc ggcgttctgg ccgatctgct cttcagcttc ctcgcgaacg
      421 tcatcccctt caatgggcag acgaccgatg acatcctgac gaaaacgaag atgccgttcc
      481 agccgtcggc ctgggtctgg gcgatctggg tggcgatctt catcctgatg gcggtctacg
      541 tcgtctacat gatcttcccg ggtgggcggc acagcgatcg cacgcgccgg atcggaccct
      601 tcttcctgat cgtgaacgtc gcgaatgtcc tctggctctt cttctggcac tggaaccagt
      661 tcctgctctc cctgatcctg atggcgatcg ttctcgcttc gctggcaatc atctacctga
      721 tggccaacag caaggacgac gagggaaaca aaccgacccg gacgcagctg ctcatggtgc
      781 ggatcccgtt cagtgtctat ctcggctgga cgacggtcgc gacgctcggg aacctgatgg
      841 tctggctgca gcaccgcggc tgggagggtg cgccgttttc cctgcgggtg tgggcggtga
      901 tcttcatgat cgttggcctg ctcgccgccg ccgcgttcgc cgccttccgg caggacgcgg
      961 cctacgcgat cgtcttcgtc tgggcatacg tcgcggtcgc ccagcatcag tgggatatca
     1021 gcaagctcgt ctcgatcttt gccgggatcg gcgccgtcgt ggcggctggc ctcgcggtga
     1081 tggcattcat gctctcgttc gaccgccgcg cgatgtcggg aatgtacgtg gaagagcatc
     1141 gcgagaatcc gctgccgctg gagtaaaaag gggcgtttgt acgcggctaa ccaccgcctg
     1201 ttccccttgc gccgggccca ttctcgggta ggatggtcgc tacagcgcat caggggtcgt
     1261 cggctccatc tcgcgcttgc cggcgcaacg ccaaccgggg ccgcgaggtc acacggggag
     1321 acctgtcacg catgaatgtt cggaagaatg gccggagcaa tacgccgcgt acagccccga
     1381 ctgtcggcgc gcccgggcga ttgtcctggc gcccttccct gacgagtatc cgtgacatgc
     1441 gcgcgccaga ggcgctggag aacccgaact tccgtcgcta ctggtcgagc caggcggtgg
     1501 cgctggcggg cacctggatg cagaacacca gcgcctcgct ggtcgtgctc tcgctgacat
     1561 cctccgcgct gctgatcggc gcgatcaacg tcatctccgc gctgccgctg ctcctcttca
     1621 gtctcttcgg cggcgtcgtg gccgatcgct tcgatcgccg caagatcctg atgacgacgc
     1681 agagcctcct cggcctgatc tccctcgtct acgcgtccct gatcttcagc gacaagatcg
     1741 agttctggca cattctggtg atcgccgcgg tttcggggtc cgtcgccgcc ttcgatctcc
     1801 cggcgtcgca ggcgttcgtc tcggagctcg tcaagcggga ggatctgccg caggcgcttg
     1861 cgctcaactc ggcgtccttc aacgcgacac gcacgatcgg tcccgccctc gccggtgtcg
     1921 tgatcggtct cttcggcacg gcctgggcgt tcgtgctcaa ttccgtcacc ttgctggcgc
     1981 cgatctcgac gctctacggg ttgcggaagg tgatgacgaa accgatccgt gcggcacgcg
     2041 cctcgggagt cacccagttg cgggccggtg tcgctcacat ccggaagtcg gaggaactga
     2101 cgggcctgat cctgctgacg acgatcttct ccttcttcgt ctttccgaac ctgctcgtgc
     2161 tgatgccgct ctacatcacc gacgtgctcg gcgggtccga cacctgggtc gcgatcatga
     2221 tctccgtgct cgggatcggc tccctgatcg gctcgatcgc gatgatccgc ggcagtcgcc
     2281 tcgaggtcgc cgccggtcgc cgcttgcgga cctcgatggt cggcctgacc gttggcctga
     2341 cctggctggc gctctcgccg aatccgtggg tggcgatccc cggcgtcatg atctcgggat
     2401 actcgttcgc gacgggcaac acgcagatct cgacccgtgt ccagcaactc gcgccggacg
     2461 aactccgcgg tcgggttctc agtgtgaacg gacttgcctt caacggcgtc atgcccttcg
     2521 cgacgatcgc cgtctccggt ctctccgagg tcgttggcca gcatgtcgtg atgggtggtt
     2581 gcgcgatatg cctcgcggtc ggttccttcc tgatctggaa gcggtacgcg tggaaggcct
     2641 tcctgccgaa ctcgaccccg atccacgttg cgtcggcgac gcccgtcatc gccggtcact
     2701 gacccgcacc ccgggtcggc tccgctgacc tgcgtccgat ttgcggcggg gcatgaaccc
     2761 caacgctgcc ccataaaccg agcggggcag agaacggatt gcggtcggcg cttctcgaag
     2821 gggggacata aaagtccccc cgtacgcacg gccgcgttgt cgacggcgtc cgtgcgggag
     2881 cctgccccga gcgcagccga gggggcgggg cccccgtgtc ccgcctcaaa gaacgcgcgt
     2941 ggcgaggagc gcgctccatc accgtcccgg agccgatccg tttccgcagg gcacgttgca
     3001 cggaatggcc attgtcgggt ccggcggagt ttaccctgag cctgtcgaac gggccggatg
     3061 tgcttcgcac ggggattcct cgacaagctc gaaatgacaa acctctatgc gacagcgttg
     3121 ggcatgaaac cgcgccctac caacgccgca tcgtcgcgac gtccggggag tggcccgggc
     3181 gagatcgtgt cgtccataaa ctgtctgaag atgccgcctt gatcacgaag caatccgcgt
     3241 atgtgaaaat cgggaccctg catctcttgc atccaggatg tggtccgcta ggatgatgct
     3301 atccacgcat cgacggcgtg gttctttcat ctacgttctt gcacatcgtg cttgaggatg
     3361 ttcgccgccg atgacgatcc gctctatgac caccagggcc ccttcgacgg ccacggccgc
     3421 ggttccgacc accgatcagg aggtgacgga cgaacctccg ccggtagagg ggcgcgtgcc
     3481 caagggggtg atgccccgca gcctccgcct cgcgaagccg tggaaccgat cggcgccacc
     3541 aaggacggcg cccgtcgtcg ggcatcccga cgagatcgct cctcactccc cgcgtcttgc
     3601 gctacgggac ctccaggttc cgaaggcgct gcagcatccc ccgtttcgcc ggttctggtc
     3661 cgggcaactg gtcgcgttga tcgggatgtg ggggcagagc acggcctcca atctcgtctt
     3721 cctgaccctg acgtcgtcgg cgttcctgat cgggctcatc aacatcgtct cggccgtgcc
     3781 gatgctgttg ctcagcctct tcggcggcgt gattgccgac cgcttcgagc ggcgcaagat
     3841 cctgacgatg acccagaccg tgatcgcgag cctctccgtg gtctgggcga tcctgatctt
     3901 cacggatagg atcgcctact ggcacgtcct cgtgatcgcg gcagtcggtg gcgtcgtcgt
     3961 ctcgttcgat ctcccggccg gccaggcgtt tctctcccag atcgtgcgcc gagaggacat
     4021 gacggaagcg atcgcgctca attcggcatc gatcaacgcc acgcgcatct tcggtccgat
     4081 cctcgccggg atcctgatcg gtgtcgtcgg gaccggcgtc gccttcgtcg cccattcgac
     4141 ggcggtgatg atcttcgtga tcacgatctt ctccctccgg gcgatggtgc cgcattcacg
     4201 accagtgggc tcgcgcgtcc ccggcatcac ggcgttgcgc gacggattac gtcacatccg
     4261 gcacagcgac gagttgatcg gcctcgtcgg cgtgaccgcg atcttctcgt tcctcgcggt
     4321 gccgccgctg ctcgtgctca tgtcgctgta tgtcacgaag acgctcggtg gcagtgatgc
     4381 ctgggtgccg acgatgacgt cgatcttcgg cgcgggatcg ctgatcgcgg cgatcgcgct
     4441 cttccggggc agccggctcg aggcggctgc gggccgccgg atgcgcctga cgatggccgg
     4501 actcgcggtc ggcctgatgt ggctggcgct ggcgccgaat ccctggctgg cgattccggg
     4561 catcctgatc gccggttgct cgttcgagat gggattgatc caggtccaga cgcgggtaca
     4621 ggcgctggcg ccggacgatc tgcgcggtcg cgtcctgagc gtgaacgggc tcgccttcaa
     4681 cggggtgatg cccttctcca cgctcacgat ctcgaccgca acgacggaac tcggcgcacc
     4741 gcttgtgctc ggcgcatgtt cggtgctggt cgcgatctgc tcggtgctga tctggaaacg
     4801 ttacacgtgg aaggcgttcg tgccggctcc ggcaatctga atctgcccgg ggcatcgcga
     4861 accggagcgt gtcctggcag ctgcatcgga gcgcagccga aggaatccgc ccctgcggaa
     4921 gcgcctgacg acgttgcgcc ggcgcgtcgc tcggaggacg tgttgggagg aacgcgcctc
     4981 ccgggaggac atgatgtgac gggcggtttc cccatccacg tcctcccgag ctcgtcgagg
     5041 gatcagccgg cgcccacgtg cttcccgccc aggacgggtt cccctgggga agggaggcac
     5101 gcgaggcgtt gccagtggtt ccggccgatc accagagccc cggtctgcgg gtcagcgcac
     5161 gggtgcggcg accgcggcgt ggaggtcgag caaccccggt gtgcgctcgc gcggtccgcg
     5221 tccgaagccg cattcggtgg ccacgccgaa gttcgggacg aacttctccg ccgccttcgc
     5281 gcggcgctcc gcgccttcga cgccgtcctc atgatggatc aggccgaggt agacctcggt
     5341 gtccgccggc cacggcacgt tcgcgagcgg cgcgaagaag gcctcgtcgt cgcgctcgat
     5401 cggcactggc aggtggaccc aggtgatcgg ccgcgtcgcg ttctcgaaga tcccggtcag
     5461 cacggcggca agcaggccgg catccttcgg ctggatgaag tgactctcct cgacatcgcc
     5521 atagca
//
LOCUS       DASTMD010000290         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_25859, whole genome shotgun sequence.
ACCESSION   DASTMD010000290 DASTMD010000000
VERSION     DASTMD010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25859"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..465)
                     /locus_tag="VFQ54_04295"
     CDS             complement(130..465)
                     /locus_tag="VFQ54_04295"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0164241.1"
                     /translation="MKYMLMIFDPSDYWETTPEADQEREFQEHAAFGRWLDEHGIVHS
                     GEALQSPAVATALRRNGDEVVVSDGPFIELKETIGGFYLFEAPDLDVALDVARRCPAN
                     NVELRPVWG"
     gene            complement(548..1282)
                     /locus_tag="VFQ54_04300"
     CDS             complement(548..1282)
                     /locus_tag="VFQ54_04300"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0164242.1"
                     /translation="MIPLRDDNRGRHSQPYVTYAIIASNVLVYLYQARLPHDRLVAFI
                     TRWGAVPATITGGHDWWTLLTSLFLHASWVHLGGNMLFLWIFGDNVEDVMGHISFGTF
                     YVLVGLCASAAQVWLGGPDSHVAIIGASGAISGVLAAYMIFFPRGRVSTLVFLKFFVT
                     VLMLPAWVMIGVWIALQILQGFVSTTVQTAESTSVAYLAHVGGFVGGLALVWLFADRD
                     RLADQRIARQVIPEAIRWRSKERSLG"
     gene            1730..1990
                     /locus_tag="VFQ54_04305"
     CDS             1730..1990
                     /locus_tag="VFQ54_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0164243.1"
                     /translation="MLTLREARQRRHMSQQVLADRAGVARVTVSQIELGKSGPRPHIA
                     RRLSQALDIAPGEIAEFGKAARSMRLPTPSILAVRAEVELVS"
BASE COUNT          449 a          622 c          651 g          322 t
ORIGIN      
        1 tcgccctgat cccaccacgg cgacgcctcg ccaatgatca tcagcatgta cttcatcgat
       61 aggttcctgt ttccgtttcg cggggcggaa gctcgatcag gatcagtcac gtgaatcagg
      121 aaaaccggtt cagccccaga cgggtcgcaa ctcgacgttg ttcgccgggc agcgacgtgc
      181 cacatccagc gcgacatcga gatcgggcgc ctcgaagaga tagaagccgc cgatcgtctc
      241 cttcaattcg atgaagggac catcggaaac gaccacttca tccccgttgc gacgaagcgc
      301 ggtcgcgacc gccggagatt gcagcgcctc gcccgagtgg acgatgccgt gctcatcgag
      361 ccaacggccg aatgccgcgt gttcctggaa ttcacgttcc tgatccgcct cgggagtcgt
      421 ctcccagtaa tccgacgggt cgaagatcat cagcatgtac ttcatgccct gctcctttcc
      481 tcatcaagaa gacgaacggc cgaaggggga atcgacaacc cgcgcgaacc gattttccgg
      541 aatccgctca accgagcgaa cgctccttcg agcgccagcg gatcgcctcc ggaatgacct
      601 gccgcgcgat ccgctgatcc gccagacggt cgcgatcggc gaagagccag accagcgcca
      661 ggccaccaac gaagccgccg acatgggcca ggtaggcgac actcgtggac tccgcggtct
      721 ggacggtggt cgagacgaat ccctgcagta tctggagtgc gatccagaca ccgatcatca
      781 cccaggccgg cagcatcagc acggtgacga agaacttgag aaagacgagt gtcgatacgc
      841 gaccgcgcgg gaagaagatc atgtacgcgg cgagcacgcc ggatatcgcg ccgctcgcgc
      901 cgatgatcgc gacgtggctg tcggggccac cgagccagac ctgcgccgcg gacgcgcaca
      961 acccgacgag gacatagaag gtgccgaagg agatgtgccc catcacatcc tcgacgttgt
     1021 cgccgaagat ccagaggaag agcatattgc cgccgagatg cacccaggag gcgtggagga
     1081 agagggacgt caggagcgtc caccagtcgt ggccgccggt gatggtcgcc ggaacggcgc
     1141 cccacctcgt gatgaaggcg acgaggcgat cgtgcggcaa ccgcgcctgg taaaggtaga
     1201 ccaggacgtt gctcgcgatg atcgcgtagg tcacgtacgg ctgtgaatgg cgtcctcgat
     1261 tgtcatcacg cagcggaatc atgcgaagtc attccatctg tactggatgg cggtcctgtt
     1321 cctcgcggac gcattgccat caccgatctc ccatcatgca catggtagcc ggtggaagcg
     1381 tcgcagcgat cgcggtcgga tcgcgggaga tcgtgccaag cgggaaaagg cgagcggtcg
     1441 caaggacgat cgcgtggcgc gacaacatca ggcggaccgc acgaacaggt acgggaatac
     1501 gaatgtactg atcgtacaca ctgtccgttc ggatgcgaca acccggggtc ggaatcgggc
     1561 cgtgaaattg ccaaaacgga aagccgaacg gacacctttt ccccgccatc atgggcagat
     1621 taggattggc cgtgggagag atcgggggtt ccccgatccg attcgccgac agcccgtacc
     1681 ccgtacgccg gcgagttgca gagggatgag ggaagaggga gagcgtggga tgctgacgct
     1741 tcgggaagcg cggcaacgca ggcacatgtc gcagcaagtg cttgcagatc gtgccggggt
     1801 ggcacgggtg acggtgtcac aaatcgagct tggcaaatcg ggaccgcgac ctcacatcgc
     1861 gcgacgattg agccaggcac tggacatcgc accgggagag atcgccgagt tcggcaaggc
     1921 tgcacgcagc atgcgactgc cgactccgtc gatcctcgcc gtacgcgccg aggtggaact
     1981 cgtatcctag ccggtgcgag cgcgagaaca acgaacgggg cggtgatgac cacaactcat
     2041 cacc
//
LOCUS       DASTMD010000291         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1364633, whole genome shotgun sequence.
ACCESSION   DASTMD010000291 DASTMD010000000
VERSION     DASTMD010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364633"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFQ54_04310"
     CDS             complement(<1..196)
                     /locus_tag="VFQ54_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164244.1"
                     /translation="MSSNTSPSPGSSDRRIGDRRMWRTGMVGGWRSWPIEVYFPIFLA
                     ALLCSAIAPLLHLFLNPHFGL"
     gene            complement(159..1487)
                     /locus_tag="VFQ54_04315"
     CDS             complement(159..1487)
                     /locus_tag="VFQ54_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU0164245.1"
                     /translation="MAARAQVATPKLPGRWTRLREWWWDPEADVNIQRWLGVAVLLAV
                     IGAFLKQPLLFLLAFGVAVAGLGARLWWDNAFRNLTFTRSFSKTRAFYADELTMELTV
                     VNTKPLPITRFEVSDEVTANLDIERQVLERSERSDRRLMRSVFSLGMYERVVYRYRIH
                     CHHRGWHRFGPAKAVAVDPFGIVSRRQEIPYAEGFLVYPRIVPVTSLIVPPRQPYGDF
                     RPSQQLIEDPMRMAGVREYVPGDSPRRIHWRATARTGVMQTRVFEPTASPIAAIFLDT
                     ITFSYLWEGQNSDLLELAITVTASLSTQLLADRHQVGFYANAPIPERSRTVRVPPGRR
                     PGQTTRILENLAMLTPAFGERIEEMVVKELPRLPWGAAVVIVTTRVTEAMQRTLLRLS
                     RTSGSQRFVLVVVGDVPELVPEFRKRIAVHHMGAEEAWNVIEHISLTRLV"
BASE COUNT          311 a          512 c          557 g          244 t
ORIGIN      
        1 tcaggccgaa atgaggattc aggaagagat gcagcagcgg cgcgatcgcc gagcagagca
       61 gcgccgcgag gaagatcggg aaatagacct cgatcggcca ggatcgccag ccgccgacca
      121 tcccggtccg ccacattcgc cgatcgccga tccgtcgatc agacgagccg ggtgagggag
      181 atgtgttcga tgacattcca cgcctcctct gcgcccatgt gatgaacggc aattcgtttc
      241 cggaactccg gaaccagctc cggcacgtca ccgaccacga cgaggacgaa ccgctgcgag
      301 ccactcgtac gagaaaggcg gagcaatgtc cgctgcatcg cctcggtgac acgcgtcgtc
      361 acgatgacga cggcggcacc ccacggcaag cgcggcagtt ccttgacgac catctcctcg
      421 atccgctcgc cgaaggccgg tgtcagcatc gcgagattct cgaggattcg cgtggtctga
      481 ccggggcgac gtcccggcgg gacccgcacg gttcgcgatc gctccgggat cggggcattg
      541 gcatagaagc cgacctgatg gcgatcggcg agcagctgcg tcgacagtga cgccgtcacc
      601 gtgatcgcga gctcgaggag atcggagttc tgcccctccc agagatagga gaaggtaatg
      661 gtgtcgagaa agatcgcggc gatcgggctg gcggttggct cgaagacccg cgtctgcatc
      721 acccccgtcc gcgccgtcgc ccgccagtgg atccggcgcg ggctgtcgcc ggggacgtat
      781 tcgcggacgc cggccatccg catcggatcc tcgatcagct gctgggatgg ccgaaaatcg
      841 ccgtacggct ggcgtggcgg cacgatgagg ctcgtcacgg ggacgatgcg cggatagacg
      901 aggaatccct ccgcatacgg gatttcctgg cgtcgcgaga cgatcccgaa cggatcgacg
      961 gcgaccgcct ttgcgggccc gaatcgatgc cagccgcggt gatggcaatg gatgcggtag
     1021 cgataaacga cgcgctcgta catcccgagg ctgaagacgg aacgcatcag gcggcgatcg
     1081 gatcgctccg agcgctcgag tacctgccgc tcgatatcga gattcgccgt gacctcatcg
     1141 ctgacctcga agcgcgtgat cggcagcggc ttcgtgttga ccaccgtgag ttccatcgtc
     1201 agctcgtcgg cgtagaacgc gcgcgtcttt gagaagctcc gggtaaacgt caggttacgg
     1261 aaggcgttgt cccaccagag acgggcaccg aggccggcca cggcgacccc gaaggcgagc
     1321 aggaagagca gcggttgctt caggaaggcg ccgatgacgg cgagcagcac ggcaacaccc
     1381 agccagcgct ggatattgac gtctgcctcg gggtcccacc accactcgcg aagacgcgtc
     1441 cagcggccgg gaagcttggg agtcgcgacc tgcgcgcggg cggccatcgg ggtcagaccg
     1501 cggtgacggc ttcgaccggc accggcgttc ccgcgatgac atccgcgatc aacgcggcgg
     1561 tcgtcgtacc gaggagatcg gcctgcgccg tcgggatgac gcggtgggag agaacgggga
     1621 tggc
//
LOCUS       DASTMD010000292         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1008345, whole genome shotgun sequence.
ACCESSION   DASTMD010000292 DASTMD010000000
VERSION     DASTMD010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008345"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /gene="rsmH"
                     /locus_tag="VFQ54_04320"
     CDS             <1..782
                     /gene="rsmH"
                     /locus_tag="VFQ54_04320"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU0164246.1"
                     /translation="GKDRLIPVHGNFGELGELAAERGITDLDGILLDLGVSSFQLDQA
                     ERGFSFRFDAPLDMRFDPTQGESAADLVNRMDAEELATLLWRYGEERQSRRIAAAIVD
                     ARSGSAIATTSQLAEIISDAVGGRRGRGIHPATRSFQALRIAVNRELEMLDRVLDASL
                     SLLAPGGRLVVIAFHSLEDRIVKRFVEREVATCTCPPDQPVCTCDQVPRLKRIGKPFK
                     PSERELDENARSRSAIMRVAERLGEDEARAATGRPAERSIM"
     gene            779..2200
                     /gene="murF"
                     /locus_tag="VFQ54_04325"
     CDS             779..2200
                     /gene="murF"
                     /locus_tag="VFQ54_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU0164247.1"
                     /translation="MSDFRLEDVLTGTGGAVRGNLAPETVFPRLERNSRETKPGDLFI
                     AIKGEQFDGHDYVEHAAESGAAAAIVSAAWAEAHPDLETPLIVVDDPILALQRWASWR
                     RELMETLVIGVTGSVGKTSAKESITAVLNQRFNVYRSPGNFNSEIGLPLALLEAPMDA
                     QIMVLEMGGAYAFGELALLASIAKPEIGVVTNVYPVHIERMGTLEAIAETKAELIDSL
                     TANGTAILNGDDVRVRAMAARAKGRVITYGLEEGNDIRAEAVATDGWKGTSFAVTIDG
                     ERSFVKVPMIGAHAVYIALVALAVGYSFDMDVAEMISGLQDPSIQVRLLFVPGPKGSQ
                     LIDDTYNASSPSVLSALGVLKEVPANRRIAVLGEMRELGDLAEQEHILVGERAGDVVD
                     VLVTFGDYAPIIADAARAVAGDRVEIHSYLERERDQIIRFLENELQEGDVVLLKGSRG
                     LKMENIVAAIRADAGQSRSASQA"
     gene            2197..>2529
                     /locus_tag="VFQ54_04330"
     CDS             2197..>2529
                     /locus_tag="VFQ54_04330"
                     /inference="COORDINATES: protein motif:HMM:NF022030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164248.1"
                     /translation="MSLPIFAGLPPVLLQASVMPTNPNENMAVSLAIAALTFVITIII
                     GRPIITYLRKRKMGKKIRIEIQDTHFAKAGIPTMGGIMFCASAAVITLIFNTPGRLST
                     LLPVAVLLA"
BASE COUNT          462 a          778 c          842 g          447 t
ORIGIN      
        1 tcggcaaaga ccgcctcatc ccggtgcacg ggaactttgg cgagcttggg gaactggcgg
       61 cagagcgagg tatcacggat ctcgacggga tcctgctcga cctcggcgtc tcgtcgttcc
      121 agctcgacca ggcggagcgt ggcttctcct ttcgcttcga cgcgccgctc gacatgcggt
      181 tcgatcccac gcagggcgag tccgccgccg atctcgtcaa tcgaatggac gccgaggaac
      241 tggcaacgct tctctggcga tacggcgagg agcggcagtc tcgccgcatc gctgccgcga
      301 tcgtcgatgc gcgctccgga tctgcgatcg ccacgacatc gcaactcgcg gagatcatca
      361 gtgacgccgt cggtggccgg cgtggccgcg ggattcatcc ggcgacacgg tcgttccagg
      421 cgctccggat cgcggtcaat cgcgaactgg agatgctcga ccgcgtgctc gacgcgtcgc
      481 tctccctgct tgcgccggga ggacgtctcg tcgtgattgc cttccattcc ctcgaggaca
      541 ggatcgtcaa gcggttcgtc gagcgcgagg tagcgacatg cacatgcccg cccgatcagc
      601 ctgtctgcac ctgcgaccag gttccgcggc tgaagcgaat tggcaaaccg ttcaaaccct
      661 cggagcggga actcgacgag aatgccagaa gcagaagcgc gatcatgcgc gttgccgaga
      721 gactcgggga agacgaggcc cgcgcggcga ccgggcgacc agcagagagg agcatcatgt
      781 gagcgatttt cggctggagg acgtgctcac gggaaccgga ggagcggtgc ggggaaacct
      841 ggcgccggaa acggtattcc cgcgtctgga gcgcaactcg cgggaaacga agccgggcga
      901 tctcttcatc gcgatcaagg gcgagcagtt cgacgggcac gactacgtcg agcatgcggc
      961 cgaatccggt gcggcggcgg cgatcgtttc cgcggcctgg gccgaagctc atcctgatct
     1021 cgagacaccg ttgatcgtgg tcgacgaccc catcctcgcg ctccagcggt gggcttcgtg
     1081 gaggcgcgaa ctgatggaga cactggtgat cggtgtcacc ggcagcgtcg gcaagacgag
     1141 cgcgaaggaa tcgatcaccg ccgtcctcaa ccagcgattc aatgtctatc gcagtcccgg
     1201 caacttcaac agcgagatcg gattgccact ggccctgctc gaggcgccga tggatgccca
     1261 gatcatggtg ctggagatgg gcggggcata cgcgttcggt gaactcgccc tgctcgcctc
     1321 gatcgccaag ccggagatcg gcgtcgtcac gaacgtctat cccgtgcaca tcgagcggat
     1381 ggggacgctc gaggcgatcg cagagacgaa ggccgagctc atcgattccc tgaccgcgaa
     1441 cggcacggcg atcctcaatg gtgacgatgt tcgggtgagg gcgatggcgg cgcgcgcgaa
     1501 gggacgcgtc atcacctacg gtctggaaga gggaaacgat atccgggccg aagccgttgc
     1561 aactgatggc tggaagggta cgtcgttcgc ggtcacgatc gacggcgagc gcagcttcgt
     1621 gaaggtgccg atgatcggcg cccacgccgt ctacatcgcc ctggtggcgc tcgcggtcgg
     1681 gtacagcttt gacatggatg ttgccgagat gatctcggga ttgcaggacc cctcgatcca
     1741 ggttcgcttg ctcttcgtgc cgggtccgaa gggatcgcag ctgattgacg acacctataa
     1801 tgcaagctcg ccgtcggtgc tttcggcact gggcgtgctc aaggaagtcc ccgcgaaccg
     1861 gcggatcgcc gtgctcggcg agatgcgcga actcggcgat ctggcggagc aggagcacat
     1921 cctcgtcggg gagcgcgccg gtgacgtggt ggacgtgctc gtcacgttcg gggactacgc
     1981 gccgatcatc gcggacgctg cgagggcagt cgcgggggat cgcgtcgaaa ttcactccta
     2041 cctcgagcgt gagcgcgacc agatcattcg ttttctcgag aacgagctac aggaaggcga
     2101 tgtcgtgttg ctgaaggggt cacgcgggtt gaagatggaa aacattgtcg cggcgatccg
     2161 cgcggacgcc gggcaatccc ggtcggcgtc gcaggcatga gtctccctat cttcgccggg
     2221 ctcccgcccg tgctgttgca ggcatcggtg atgccgacca atcccaacga gaacatggcg
     2281 gtctcgctgg cgattgccgc cctgacgttc gtgatcacca tcatcatcgg ccgcccgatc
     2341 attacctatc tccgcaagcg caagatgggc aagaagattc ggatcgaaat ccaggacacc
     2401 catttcgcca aggcgggcat cccaacgatg ggcggcatca tgttctgcgc gtcggcggcg
     2461 gtgatcaccc tcatcttcaa cacgccgggg cgactttcaa cgctgctgcc ggtcgccgtc
     2521 ctcctcgct
//
LOCUS       DASTMD010000293         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1098481, whole genome shotgun sequence.
ACCESSION   DASTMD010000293 DASTMD010000000
VERSION     DASTMD010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098481"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            516..1616
                     /gene="prfA"
                     /locus_tag="VFQ54_04335"
     CDS             516..1616
                     /gene="prfA"
                     /locus_tag="VFQ54_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU0164249.1"
                     /translation="MQSFRANLDEMERRYTELDHLMADPAVATDPERLMELGRERAEL
                     EGLVDAWRRHREIEEQIAETELMAEDSDRELAELAQDELRNLRAQTEETEQAIRRFLV
                     PKDPNDEKNVIVEVRAGTGGDEAALFAADLFRMYTRYAEKMRWKVEILSSTETDGGGF
                     REIIFEVRGKGAYSYLRYESGVHRVQRVPTTESQGRIHTSTASVAVLPEAEEVDVHID
                     DRDLRVDVFRSTGHGGQSVNTTDSAVRITHFPSGIVVSCQDEKSQLKNKLKAMTVLRS
                     RLYDLEQHRLAAERGDARRAQIGSAERSEKIRTYNFPQDRITDHRIKKNLSNIPAVLN
                     GEIDDLVLDLRAADEAERLADAGFVEADAIPA"
     gene            complement(1713..2135)
                     /locus_tag="VFQ54_04340"
     CDS             complement(1713..2135)
                     /locus_tag="VFQ54_04340"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEU0164250.1"
                     /translation="MANVQNAEIGRTLLIRLDPGEDLLGGLREAVTASGVRQGVFVTG
                     VGSLTSYHLHVVETTNLPPGNVFWKESGPFDIVAVTGAVLGGRVHAHLTVSNDERVMG
                     GHLEPGCNVLTFAFITLLEMHGDDISAWDTPGPLALTQ"
     gene            2328..3059
                     /locus_tag="VFQ54_04345"
     CDS             2328..3059
                     /locus_tag="VFQ54_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU0164251.1"
                     /translation="MERIAIISDIHGNMTALRTVLAEIDDRGIQRIINLGDLAGKGPD
                     GDQVVDICRERCEANVRGNWDEALATTTGRDWATPVQEQLGPERCAWLGSLPLAYDIP
                     FARKRIRFVHASPQGVFHRVYQHGDDEPKLAMFDTTEHTDPDFVPDVVGYGDVHTAYI
                     RTFRNRILFNVGSVGNPLDLTMAAFAVLESSGPTIGINIVRVPYDIEAELRRARESGM
                     PAYDEYAWELTTARSRNDMPAAVAE"
BASE COUNT          603 a         1064 c          935 g          503 t
ORIGIN      
        1 tgatgtggcg ccggcgcctg gagcaaaggg tgaaacggtg accgcatcgc cgttccgtca
       61 tcccaggctt gtcgaggaat cgcttggtat gcgcacgagg cctgcccgcc ggaggcagga
      121 cattactccg tccagaccgg gatggcgccg cgggaggcat cccgactgtc ggtccggact
      181 ccgcagtcca gcgccacgaa cgaccggatc tcccgcggag caccgccgcc aatcggcggg
      241 ggaacccatc tcgttcagga cgataaccgt atatacttat gtcatcgggg attcgccgtt
      301 catcctgcgt cgatacgacc ctcggatcgg acgtttcagc cgatccgggc atcgatcacg
      361 cacgggatgc aatcggcgcc cgtccgcggt tcgcaaaccg cccaacacat gcattccatt
      421 aatcgctacg ccgacgtctg ccgcgctttc tcgatcgatc tgcttctgcg cggtcgccgg
      481 catcggagaa cactatcttg gccgcttccg caacaatgca gagtttccgc gccaatctcg
      541 atgagatgga gcgccgctat accgaactcg atcacctgat ggctgatccc gccgtcgcca
      601 ccgatcccga gcggctcatg gagctcggtc gcgaacgcgc cgaactcgaa ggactcgtcg
      661 acgcatggcg tcgccaccgc gagatcgagg agcagatcgc cgagaccgag ctgatggccg
      721 aggacagcga tcgcgaactc gcggagctcg cccaggatga actccgcaat ctccgcgcac
      781 aaaccgagga gaccgagcag gcgatccgcc gcttcctcgt ccccaaggac ccgaacgacg
      841 agaagaacgt gatcgtcgaa gtccgggctg gcaccggtgg cgacgaggcg gcgctcttcg
      901 ccgccgacct tttccggatg tacacccgct acgcggagaa gatgcgctgg aaggtggaga
      961 tcctctcctc gaccgagaca gacggcgggg gattccgcga gatcatcttc gaggtccggg
     1021 gcaagggcgc gtacagctat ctccgctacg agagcggcgt ccatcgcgtc cagcgcgtcc
     1081 cgaccaccga aagccagggc cgcatccata cgagtaccgc gagtgtcgcc gtgctgccgg
     1141 aagccgagga agtcgacgtc cacatcgatg accgcgatct gcgggtcgac gtcttccggt
     1201 cgaccggtca cggtgggcag tcggtcaaca cgaccgactc cgcggtccgc atcacgcact
     1261 tcccgagcgg aatcgtggtc tcctgccagg acgagaagag ccaactcaag aacaagttga
     1321 aggcgatgac ggtccttcgc tcgcgcctct acgatctcga gcagcatcgc cttgccgcgg
     1381 agcggggaga cgcccgccgg gcccagatcg gttccgccga gcgcagcgag aagatccgca
     1441 cctacaactt cccgcaggat cgcatcaccg atcaccggat caagaagaac ctgagcaaca
     1501 tcccggcggt gttgaacggt gagatcgacg acctcgtcct cgatctccgc gccgccgacg
     1561 aagccgagcg tctcgccgac gccggcttcg tcgaagcgga cgcgatcccg gcctgaagcc
     1621 ggccccccgg tatcaggcaa ccttggaacc aagggcaact acgacctcgt agcgagaaca
     1681 gaacgaacgg ggacaagccg cgaggggacg ggttattgcg tcagcgccag tggacccggc
     1741 gtatcccacg cggagatatc gtccccgtgc atctcgagga gcgtgatgaa ggcgaaggtc
     1801 agcacgttgc agccgggctc gagatgaccg cccatcaccc tctcgtcgtt tgataccgtg
     1861 aggtgggcat ggacccgtcc accgagcacc gcacctgtca ccgcgacgat gtcgaaaggc
     1921 ccggattcct tccagaagac attccctggc ggcaggttcg tggtctccac cacgtggaga
     1981 tggtagctcg tcaacgatcc cacgccagtc acgaataccc cctggcgcac cccggatgcg
     2041 gtcaccgcct cccgcaaccc gccgaggagg tcctcccccg gatcgaggcg aatcaggagc
     2101 gtgcgtccga tctcggcgtt ctggacattg gccatctcgt ttcctctctc cgcagtgacg
     2161 acaggatttc ccgatgagac gagtatgaca ccggcgtgat gcgggaccag ggcgagggtc
     2221 gccgtcctct tctctccgcg cgatcgagcc cgcttcacga gggcgaagca gtacgattcc
     2281 agcctatact tcgcttccgt attgccgatc ggaggggttg atcaccgttg gaacgcatcg
     2341 ccatcatctc tgacatccat ggaaacatga ccgcgctgcg gaccgtcctc gccgaaatcg
     2401 acgaccgcgg catccagcgg atcatcaatc tcggtgatct cgcggggaaa ggtccggacg
     2461 gggaccaggt ggtcgacatc tgccgcgagc gctgcgaggc caacgtgcgg ggaaactggg
     2521 atgaggcgct cgcgacgacg accggcaggg actgggcgac gcccgtccag gaacaactcg
     2581 gtcccgagcg atgcgcctgg ctcggctccc tgccgctcgc ctacgacatc ccctttgccc
     2641 gcaagcgcat ccgcttcgtc cacgcctcgc cgcagggggt ctttcaccgc gtctatcagc
     2701 atggcgatga cgagccgaag ctggcaatgt tcgacaccac cgaacacact gatccagact
     2761 tcgtccccga tgtggttggc tacggtgacg tccacaccgc gtacatccgc accttccgca
     2821 accgcatcct gttcaacgtg ggcagcgtcg gcaatccact cgatctgacc atggccgcgt
     2881 ttgcggtcct cgagtccagc ggaccgacga tcgggatcaa catcgtccgc gtgccgtacg
     2941 acatcgaggc ggaactccgg cgcgcccgcg aatccggcat gcccgcctac gacgagtacg
     3001 cgtgggagct caccaccgcc cgctcccgga acgacatgcc cgctgcggtt gccgaatgac
     3061 cacgaccctt caggacctcc tgcgcacctc aaccgaccgt ctcac
//
LOCUS       DASTMD010000294         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2258753, whole genome shotgun sequence.
ACCESSION   DASTMD010000294 DASTMD010000000
VERSION     DASTMD010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258753"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VFQ54_04350"
     CDS             <1..1133
                     /locus_tag="VFQ54_04350"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEU0164252.1"
                     /translation="IASQRIREIAGAGDTVARLGGDEFTVILSDPETRSRPAEIAARL
                     VAALSAPFPILGREAIVTVSVGVAISQGHHLRADDLLRAADIAMYQAKERGRNGFVVF
                     DESMRRMIAHRVELESDLQQAVTNGEFVLFYQPEIDLRSGRISGVEALIRWNHPLHGL
                     INPAEFIPLAEETGLIIPLGRWVIEEATRQAREWMAQFPSSPLVMNVNLSARQLQDRD
                     LASDICDTLRKHELPGHMLRLEITETTLMENRESNIAALSSLRARGVQIAIDDFGSGY
                     SSLGYLKHFPVDVLKLDRSFIHDSQRPKQDDAIIHAVTTLAHGLDITITIEGIETRDQ
                     LARVKSMGCDRGQGYFFSRPLTPAQMPELLELEAPGVRPLPR"
     gene            1321..>1553
                     /locus_tag="VFQ54_04355"
     CDS             1321..>1553
                     /locus_tag="VFQ54_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164253.1"
                     /translation="MATSSSPPIDALRELSIRYLQDLLRFDTTNLPGNERLAADYIAA
                     IFDAEGIAYEIVDSAPNRASIVGRIRAANPTARP"
BASE COUNT          282 a          544 c          468 g          259 t
ORIGIN      
        1 tcatcgcaag ccagcgaatc cgtgagatcg ccggagccgg ggacacggtg gcgagactcg
       61 gcggtgacga gttcacagtg atcctgagcg atcccgagac gcggtcgcgt ccagccgaaa
      121 tcgccgcccg actcgtcgcg gcgctgagcg ccccgttccc catcctcggc cgcgaggcga
      181 tcgtcacggt gagcgtcggc gtcgccatca gccagggtca tcacctgcgg gcggacgacc
      241 tgttgcgcgc cgccgacatc gcgatgtacc aggcgaagga gcgcgggcgc aacggcttcg
      301 tggtcttcga cgagtcgatg cgacggatga tcgcgcatcg cgtcgaactg gagagcgatc
      361 tccagcaagc ggtgacaaac ggcgagttcg tgctcttcta ccaaccggag atcgacctcc
      421 gcagtggccg catctccggt gtcgaggcgc tgatccggtg gaatcacccg ctccacggtc
      481 tgatcaaccc ggccgaattc attcccctcg ccgaggaaac cggcctcatc atcccgctcg
      541 ggcggtgggt gatcgaggag gcaacccgcc aggcgcggga atggatggcg cagttcccga
      601 gcagcccgct ggtgatgaac gtcaatctct cggcccggca gctgcaggac agggatctcg
      661 cctccgatat ctgcgacacc ctgcgaaaac atgaactccc cggtcacatg ctgcgcctgg
      721 agatcaccga aacgaccctg atggagaaca gggaatccaa catcgccgcc ctctcctccc
      781 tgcgcgcacg gggcgtgcag atcgcgatcg atgatttcgg cagcggctac tcgagcctcg
      841 gttacctgaa gcacttcccc gtcgacgtgc tgaaactcga tcgctcgttc atccacgatt
      901 cgcaacggcc caagcaggac gacgcgatca ttcacgccgt gaccacgctc gctcatggcc
      961 tggacatcac gatcacgatc gaggggatcg agacccggga ccagctcgcc cgcgtgaagt
     1021 cgatgggctg cgatcgcggc cagggctact tcttctcccg tccccttacc ccggcgcaga
     1081 tgccggaact cctggaactg gaagcaccgg gcgtccggcc actcccccgc tgacgaacgg
     1141 cgattccgca ccgcccgcca gtcatcacct gaccaaatcg agcgcccctg cttcgtacgc
     1201 gggaatcaag ggtgcctcgc ggcttccgtg cccgtggtct ggcgcggatc gacggtttgg
     1261 cggatgatct cggagaagca ctcttcgcac ggtcgacata atcgaaaggg tctctcgcct
     1321 gtggccacgt cctcgtcacc cccgatcgac gctctgcgtg agctttccat tcgctacctc
     1381 caggatctcc tgcggttcga cacgacgaat ctccccggta acgagcgcct cgccgccgat
     1441 tacatcgccg cgatcttcga tgccgagggc atcgcctacg agatcgtcga ctccgcgccg
     1501 aaccgggcga gcatcgtcgg ccgcatccgg gcggccaatc cgaccgcgcg acc
//
LOCUS       DASTMD010000295         1700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2169385, whole genome shotgun sequence.
ACCESSION   DASTMD010000295 DASTMD010000000
VERSION     DASTMD010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2169385"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="VFQ54_04360"
     CDS             complement(<1..1553)
                     /locus_tag="VFQ54_04360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164254.1"
                     /translation="MVEPRPAEPIPKPNLNRIRILDTVHSPESASPNLSNGVTRSGSL
                     AGPDLIAVLDTLGLTMAVTYAPGESLTKRPLDRLWRRELEHYPSLRPRMVSLSIVVLT
                     TILFYYQYYAISGVSDQVIAETGMSFIFFVNINVVSALASALTAVAAGWADRYGRANI
                     VTVGVLLCALVCLLGFPFVHSAWGVGILYTLLGAIEGVVLVATPAIVRDFSPQLGRAS
                     AMGFWTIGPVAGSLVASFVITHTIEHLTAWQDQYIIAGVAGLVVFVIALLWLRELSPG
                     LRDQIMVSEHDRALVEARAKGIDVEAALKKPFRQMLRLDIVGSAIAISLLLFIYYAAV
                     GFFPLFFQTTFGYSTSTANSLGNWMWGVQTIALIAVGWTSDKLRVRKPFMLVGALGAI
                     VTTIALISRTTHPETSYTTFAVILVCLAFSLGLTFAPWMASFTETIERRNPALAATGL
                     SLWGLILRTVVTIATFILPFVVHHVTTIVKDGPTVQAAVAGQDPALNATQNAIVKAVA
                     ADSSIVDKV"
BASE COUNT          352 a          521 c          558 g          269 t
ORIGIN      
        1 tgcaccttgt cgacaatgga cgagtcggcc gcgaccgcct tcacgatcgc gttctgcgtg
       61 gcgttcaagg cgggatcctg ccccgcgacg gcggcctgga cggtcggccc gtccttgacg
      121 atcgtcgtta cgtggtgcac gacgaaagga agaatgaacg tcgcgatcgt gacgacggtc
      181 cgcaggatca gtccccagag ggaaaggccg gtggcggcaa gcgccgggtt gcgacgctcg
      241 atggtctcgg tgaagctcgc catccacggc gcgaaggtca gacccagcga aaacgcgaga
      301 cacaccagga tgacggcgaa cgtggtgtag ctcgtctccg gatgcgtggt ccggctgatg
      361 agcgcgatgg tggtgacgat tgcgccgagc gcaccgacga gcatgaaggg tttacgtaca
      421 cgcaacttgt cggaggtcca gccgaccgcg atcaacgcga tggtttgcac gccccacatc
      481 cagttgccga gcgagttcgc cgtcgacgtg ctgtaaccaa aggtcgtctg gaagaacagc
      541 gggaagaagc ccaccgccgc gtaatagatg aacagcagca ggctgatcgc gatggccgag
      601 ccgacgatgt ccagacgcag catctggcga aagggcttct tcaaggcggc ttcgacgtcg
      661 atgcctttgg ctcgtgcctc gaccagtgcg cgatcgtgct cgctgaccat gatctgatcg
      721 cgcaggcccg gcgagagctc gcgcagccac agcagcgcga tcacgaacac cacgagcccg
      781 gcgacgccgg caatgatgta ttggtcctgc caggcggtca ggtgctcaat cgtgtgggtg
      841 atgacgaagc ttgcgaccag gcttcccgcc accggcccga tcgtccagaa ccccatcgcc
      901 gaagcacgtc cgagctgggg actgaaatcg cgaacgatcg caggcgtcgc cacgagaacg
      961 acaccttcaa tggcgccgag caacgtgtac agaatcccga cgccccaggc cgaatgcacg
     1021 aagggaaacc cgagcaggca gacgagggca cacagcagga cgccgacggt gacgatgttg
     1081 gcccgaccgt accgatcggc ccaccctgcc gctaccgccg tcaatgcgct ggcgagcgcg
     1141 gacaccacgt ttatattgac aaagaagatg aacgacattc cggtctcggc gatcacctga
     1201 tcgctgacgc cgctgatcgc gtagtactgg taatagaaaa ggatcgtggt gagcaccacg
     1261 atcgacaagc tcaccatccg tggccgcagg ctcggatagt gctcaagctc ccggcgccag
     1321 agcctgtcga gcggcctctt ggtcagggat tcgcccggtg cgtatgtcac ggccatcgtc
     1381 agccccaagg tgtcgagaac ggcaatcaag tccggtcctg cgagcgaccc agaccgtgtc
     1441 acgccgttgg acaggtttgg tgaagcgctt tcgggtgaat ggacggtatc cagaatccga
     1501 atacgattca agttcggctt ggggataggt tcggctggac gtggttcgac caacggaggc
     1561 aggatcagtc gtggcaacga gccctggcga gatgcgcccg accccgccgc ccgccggcga
     1621 tggggcggcc ggaatgacac tgcgcgtcgt cccccagcag gctcgcggga aggacaagat
     1681 cgcgcgggtg ctcgcgtcgg
//
LOCUS       DASTMD010000296         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_473132, whole genome shotgun sequence.
ACCESSION   DASTMD010000296 DASTMD010000000
VERSION     DASTMD010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473132"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1674
                     /locus_tag="VFQ54_04365"
     CDS             <1..1674
                     /locus_tag="VFQ54_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164255.1"
                     /translation="AASPAASPAASPVAYDGPVGTLSVSSADFYKALNEKFQFEAAGK
                     TGGSLIEINTTDIVTVNPLLSSDVYSSEIIGLYNDVLIQSSPVDGSFVPALADSWEIA
                     ADGVTYTFHLHEGVTWSDGEPFTADDVVFSFDATVAENSISIRRADVLAVLASYKKID
                     DLTVQFVAKTPVATFLDKTVNQVGIAAKHIWESVPFDKWGTDAGSTGQDPKRVVGTGP
                     FLFKEWVQNDHVSLVKNPNYWNAANAPVNIDTFIYRVVADPNAALQSLKTGESDIFGI
                     EPSQAESLKASNPEINLSIYDTFSFGFYGPNQDPSKTKLFQEIPVRQALHYAIDRDLI
                     ASQIYLGYAIRADGSEPVPSPAYAPDKMNTIYKYDPDKAKQLLADAGWADADGDGFLD
                     KDGVKFSFECEYPEGIAAYSQQLPYMQQAWKEVGVEMFPVSIPFPTLVDHVTSSNFVM
                     ALLGFSWGVDPDQGAMFRTDAKPPAGFNLTFYSNPEYDKLNDEQNVELDVKKRIDLII
                     EQTNIVNDEVATGVTTFTKGVVASTPRVHNFFANAYGYAWAYSYAWVED"
BASE COUNT          463 a          668 c          560 g          403 t
ORIGIN      
        1 gccgcctcgc cagccgcatc ccctgcggcg tcacctgtcg cctacgatgg cccggttgga
       61 acgctctcgg tctccagcgc ggacttctac aaggcgctca acgagaagtt ccagttcgag
      121 gctgccggca agaccggcgg cagcctgatc gaaatcaaca cgaccgatat cgtgaccgtc
      181 aatccgctgc tttcctccga tgtctattcg agcgagatca tcggactcta taacgatgtc
      241 ctcatccaga gcagtcccgt cgatggcagt ttcgttcccg ctctcgccga ttcctgggag
      301 atcgcagccg acggcgtcac ctacaccttc caccttcatg aaggcgtgac ctggagcgac
      361 ggcgagccgt tcaccgccga cgacgtcgtc ttctcatttg acgccaccgt cgccgagaac
      421 agcatcagca tccgtcgcgc cgatgtactc gccgttctgg cgagctacaa gaagatcgac
      481 gatctcaccg tgcagttcgt cgccaagacc ccggttgcaa ccttcctcga caagacggtg
      541 aatcaggtcg ggatcgcggc gaagcacatc tgggaatccg ttcccttcga caagtggggc
      601 accgatgcag gttccacggg tcaggacccg aagcgtgtcg tcggcaccgg tcccttcctc
      661 ttcaaggaat gggtccagaa cgaccacgtc agcctcgtga agaatcccaa ctactggaac
      721 gccgccaacg ctccggtcaa catcgacacg ttcatctacc gcgtcgtcgc ggatccgaac
      781 gcggcgctcc agtcgctcaa gacgggcgag tccgatatct tcggtatcga gccatcacag
      841 gcggagtcgt tgaaggcatc gaatccggaa atcaacctct ccatctacga tacgttcagc
      901 ttcggcttct acgggccaaa ccaggatcca tcgaagacaa aactcttcca ggagatcccg
      961 gtccgccagg cgcttcacta cgcgatcgac cgcgatctca tcgcctccca gatctacctc
     1021 ggctatgcga tccgcgccga tggcagcgag ccggtcccgt ccccggccta cgcgcccgac
     1081 aagatgaaca cgatctacaa gtacgacccg gacaaggcaa agcaattgct cgccgacgca
     1141 ggctgggccg atgcggatgg cgacggcttt ctcgacaagg acggcgtcaa gttcagcttc
     1201 gagtgcgagt atccggaagg tatcgccgcc tatagccagc agctgccgta catgcaacag
     1261 gcctggaagg aagtcggggt cgagatgttc ccggtctcga tccccttccc cacgctcgtc
     1321 gaccacgtca cgtcgagcaa cttcgtgatg gcgctgctcg gcttctcctg gggcgtggac
     1381 cccgatcagg gagcgatgtt ccgcaccgat gcgaagccgc ccgccggctt caacctcacg
     1441 ttctattcca atccggaata cgacaagctc aacgacgagc agaatgtcga actcgatgtg
     1501 aagaagcgga tcgacctgat catcgagcag accaacatcg tgaacgatga ggtggcgact
     1561 ggtgtgacga cctttacaaa gggggtcgtc gcctcgacgc cgcgcgtcca caacttcttc
     1621 gcaaacgcct atggatatgc atgggcatat tcatacgcct gggtggaaga ttaggattca
     1681 ccccgtacca ccggaatagg tcaaaacgag agagcctgtt cccggcttcg ggagcgggct
     1741 cttccgtttt tctcagtaac cagctcacta ttcatatgaa gcaccccgtc tgtttgtgca
     1801 tgatgcctaa gttaagccaa ataggacatc tcaagctgct tgacggtttg atcaagcgat
     1861 ccatatcatg cccacatgag gccgcctctg tgcggaacac taggcaaaga cgccaccggg
     1921 tacctcaaag cgccaaccca gccacaaccg catcgaaagg tggtgtgcag aagtaacagc
     1981 acccatcctc ggatggatcg cgtgcacaaa gacgtctttc tacagaatga cttcaccgaa
     2041 ctgagggggt tcccgcatga aggattctac gatcggccag ttgctcgagg actt
//
LOCUS       DASTMD010000297         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_384424, whole genome shotgun sequence.
ACCESSION   DASTMD010000297 DASTMD010000000
VERSION     DASTMD010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384424"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VFQ54_04370"
     CDS             complement(<1..1048)
                     /locus_tag="VFQ54_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0164256.1"
                     /translation="MFENELKRQRKAAAELVDQHLGGSLNRRSFLGKAAALGISVPVL
                     TQVLGARGAFAQSASPSPVAEENVTLGSYDGKKLKISIALAESEAQVFKDVVVAGFKD
                     KTGGDIDLVNIEATDVIRTLQAQKQSGNVQLDLLVQDNNSLAQLVSGGLVEEITNAEQ
                     VMPAATIPSLKDVLQFDGKYYFLPARPNVQIAYYNSKSFDGWGLKPPTTWDDLKSVGD
                     EVKKQSGIGKLSIQGVPGGAAGVTVTQFIWQAGGDPLKINTDQGKTAFEFMQNLKPDL
                     TPQYPTATFDTTNNYLLNESVVLAQNWPFGINVIVDQGGKKEVLAYSGWAGPTGKNVL
                     VLGGDVFGIVAGTQL"
     gene            complement(1128..2003)
                     /locus_tag="VFQ54_04375"
     CDS             complement(1128..2003)
                     /locus_tag="VFQ54_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0164257.1"
                     /translation="MSTLIAPDNPGIESAPITPKRRPPYADVGFWILAIIIAVILLVP
                     LYVLLKVSLSSVAQATAPSPSYWISNPTLDNWRRLFKWDIIGPPLEHSLIVATGTAFF
                     AILLAAPAAYVISRMPSRTRYLVILAVLFTRMFPEVIIATPIASNFFRWGLNDTNVGL
                     IMAHLIRSLPLAAWIMIGSFQVIPRDLEESSAIDGASKLTTLIRVILPLGLPGIAVAA
                     IFAWLDSWNDLLYAIYLFLTQRTLPLVTYYYANRGTVTDVATFSIIMTIPVIIITLFL
                     QRWIRSGYLSGAVKG"
     gene            complement(2000..>2654)
                     /locus_tag="VFQ54_04380"
     CDS             complement(2000..>2654)
                     /locus_tag="VFQ54_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0164258.1"
                     /translation="IGITVMGVTMEMVFGLIIALMLVRAFRGRGLFRSLVLIPMGVPT
                     LVAGAAMLYFVGFNGYLNKILLQLNIINVPVYWQQSGPRGMFVIALSDLWKATPLVVL
                     ILVAGLEAIPGDVYEAAGVDGANSWQKFINHTIPLLMPAITMALILRAIDAFRIFDIA
                     MVLAGQAIPVMSSFVYFDYEAGQTNEAAAAAVILLLMIIVFVVAYIFLVARRSEVER"
BASE COUNT          520 a          767 c          892 g          475 t
ORIGIN      
        1 ggagctgggt gcccgcgacg atgccgaaga cgtcgccacc gaggacgagg acatttttgc
       61 cggtcgggcc cgcccaaccg gagtaggcta ggacttcctt cttgccgccc tggtcgacga
      121 tcacgttgat accgaacggc cagttctggg cgagcacgac gctctcgttg agcagatagt
      181 tgttcgtcgt gtcgaacgtc gccgtcgggt actgcggagt cagatccggc ttcaggttct
      241 gcatgaactc gaaggcggtc ttgccctggt cggtgttgat cttcagcgga tctccaccgg
      301 cctgccagat gaactgggtc acggtcacgc cggcggcgcc accggggacg ccctggatcg
      361 acagcttgcc gataccagac tgcttcttga cctcgtcacc gaccgacttg aggtcatccc
      421 acgttgtcgg cggcttcagc ccccagccgt cgaacgactt cgagttgtag taggcgatct
      481 gcacgttcgg acgggccggc aggaagtagt acttcccgtc gaactgaagg acatccttca
      541 gggaaggaat cgtcgccgcc ggcatcacct gctcggcgtt ggtgatctcc tcgacgagac
      601 cgccggaaac gagctgcgcg agcgagttgt tgtcctgcac gagcaggtcg agctggacat
      661 tgccggactg cttctgcgcc tgcagggtgc ggatgacatc ggtcgcctcg atgttgacga
      721 gatcgatatc gccaccggtc ttgtccttga agccggcaac cacgacgtcc ttgaagacct
      781 gggcctcgga ttccgcgagg gcgatcgaga tcttcagctt cttgccatcg taactgccga
      841 gtgtgacgtt ctcttcagcc accggcgacg gcgacgctga ctgggcgaat gcaccgcgcg
      901 cgccgagcac ctgggtcagg acgggaacgg agatgccgag ggctgctgcc ttgccgagaa
      961 aggaccggcg attgagggac ccgccgagat gctggtcgac cagttccgcg gcggcctttc
     1021 gttgacgctt cagttcgttc tcaaacatgt acttcgagcc tccttgtcga tgtattgcct
     1081 cgcggtgcgc gctcgcgcgc cgccaccaac tcagccaagg atcaggacta tcccttgacg
     1141 gcgccgctca gatatccact acggatccag cgctgcaaga agagcgtgat gatgatgact
     1201 ggaatggtca tgatgatgga gaacgtggcg acatcggtga cagttccccg attcgcgtag
     1261 tagtacgtga cgagcggcag cgtccgctgg gtcaggaaga gatagattgc gtagagcagg
     1321 tcgttccagg aatcgagcca ggcgaagatc gccgcgacgg caatgccggg aagaccgagc
     1381 ggcaggatca cacggatcag cgtcgtcagc ttgctcgcgc cgtcgatcgc gctcgattcc
     1441 tcgagatcgc gcgggatcac ctggaacgaa ccgatcatga tccaggccgc cagcggcaag
     1501 gaccggatca ggtgggccat gatcaggccg acgttggtgt cattgaggcc ccagcggaag
     1561 aagttgctcg cgatcggtgt tgcgatgatc acttccggga acatgcgggt gaagagcacg
     1621 gcgaggatga cgagatagcg cgtccgcgac ggcatgcgcg agatgacgta ggcagcgggg
     1681 gcggcgagca ggatcgcgaa gaaggccgtg ccggttgcga cgatcaggct gtgctccaac
     1741 ggcggtccga tgatgtccca cttgaacagt cgccgccagt tgtcgagcgt cgggttgctg
     1801 atccagtagg aggggctcgg tgcggtcgcc tgggcgacgc tgctcagcga gaccttcagc
     1861 agcacgtaga gcggcacgag caggatgacc gcgatgatga tcgcgagaat ccagaagccg
     1921 acgtccgcgt agggcggtcg gcgcttcggg gtgatcgggg ccgactcgat gcccgggtta
     1981 tcgggggcga tcagggtact catcgttcga cctccgagcg gcgtgcgacc aggaagatgt
     2041 aggcgaccac gaagacgatg atcatcagga ggaggatcac cgcggcggcg gcggcttcgt
     2101 tcgtttgccc cgcttcatag tcgaagtaga cgaacgaact catgaccggg atcgcctgtc
     2161 cggcgagcac catcgcgatg tcgaagatgc ggaaggcgtc gatcgcgcgg aggatgagcg
     2221 ccatcgtgat cgcgggcatc agaagcggaa tcgtgtggtt gatgaacttc tgccagctgt
     2281 tcgcgccgtc gactccggct gcctcataca cgtcaccggg aatcgcttcg agaccggcga
     2341 cgaggatgag gacgacgagc ggcgtcgcct tccagaggtc ggagagcgca atcacgaaca
     2401 tgccgcgggg accgctttgc tgccagtaca ccggcacatt gatgatgttc agctggagga
     2461 ggatcttgtt caggtagcca ttgaagccga cgaagtagag catcgcggcg ccggcgacga
     2521 gggtgggaac gcccatcggg atcagcacca gtgaccggaa gaggccacga ccgcggaagg
     2581 cgcgaacgag catcagcgcg atgatgagcc cgaagaccat ctccatcgtc acgcccatga
     2641 cggtgatgcc gatc
//
LOCUS       DASTMD010000298         5246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_384476, whole genome shotgun sequence.
ACCESSION   DASTMD010000298 DASTMD010000000
VERSION     DASTMD010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384476"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VFQ54_04385"
     CDS             complement(<1..934)
                     /locus_tag="VFQ54_04385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEU0164259.1"
                     /translation="MSVRVLCLGDVHMGRRASRVSGTYRSVDAWSRAVDIAIAERVDL
                     VALSGDIVDEESKSYEALGPLEAGIRRLDGAGIDTVAVSGNHDWDVLPRLIGASGGER
                     FHLLGRYGAWERFTLRRDNVPVLFVDGWSFPRGRVPESPLAHYRPQGGDGVPLLGLFH
                     TDLDAMVSNHAPVRSVDLWRYPVDFWLLGHIHAARVLTGPQGRGTAIYPGSPYALDPG
                     EPGTHGVWLATFQPGEPVALRPIPLSPVRYESREVDLADVVDEEGFLRALSSAMRDPT
                     FSSQSDGSLEAISLRLTFTGRSAAHQDILAWSDRA"
     gene            complement(1034..1930)
                     /locus_tag="VFQ54_04390"
     CDS             complement(1034..1930)
                     /locus_tag="VFQ54_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0164260.1"
                     /translation="MLASDRGLWLGSNEGQGFVFGMLGILAFSFTLPFSRVASPQLGG
                     LFVGIERALLASALAIVILLWRREAWPERRHWWPICIVALGTVLGFGPLSSLAMQYLP
                     AAHGVVVIGLLPAATAVMAVLRAGEHPPRSFWLACSLGVAAVVVFAIVQGAGHPQWGD
                     LLLLGAVVMASLGYAEGARLTRELGGWRVISWALVFSLPVIVMLSVVLYVWNGWPSGD
                     AEAWWSMLYLGAVSMFFGFFPWYRGLAVGGVAKVGQLQLVQPVLSLIWAALLLGEHLS
                     VGTILASLLVIASAALSRLARR"
     gene            complement(2087..2959)
                     /locus_tag="VFQ54_04395"
     CDS             complement(2087..2959)
                     /locus_tag="VFQ54_04395"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0164261.1"
                     /translation="MNLTVVQLRAFAAVIDGGGFGEAAECLAMTQPAVSHAIATLEKA
                     IGAPVLQRKPQLQPTPLGEKILPHARAALASVEAVQATVREHLGLTAGLVRLAAAPTV
                     CQGLMPELLMRWRSTHPRVEVQIFEGDDDELALWLEGGMVDAAILIDPDPMPDRGVIV
                     GRDTFQAVVRRDHPLANEVAVTLHDLLDDPFIISTGGCEAQISRIFAQAHESMVLGQR
                     VREVGTLLSMIVGHVGISIMPSLGEAMLPRDLVMVPLVPVTDRTLVFTGPMSRPWNPL
                     VGALRDACGETVLA"
     gene            3133..3639
                     /locus_tag="VFQ54_04400"
     CDS             3133..3639
                     /locus_tag="VFQ54_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0164262.1"
                     /translation="MKETAVSGRRGTLRKAENSVLVPFVLLAVIWGASFLFIKEALNG
                     FAPVQVAAGRISLGAITLVVMMMVTRRKWPRGRIWIHLVVGAIPLCVLPMLLYSWATQ
                     HLPSSLESVYNATTPLMTMLIAVLLPSAERLSRARLTGLLVGGAGVLIVLAPWHLIAD
                     TGALAGTG"
     gene            3658..4110
                     /locus_tag="VFQ54_04405"
                     /pseudo
     CDS             3658..4110
                     /locus_tag="VFQ54_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(4107..>5246)
                     /locus_tag="VFQ54_04410"
     CDS             complement(4107..>5246)
                     /locus_tag="VFQ54_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEU0164263.1"
                     /translation="PRFLKAPGIPIALIGIVVATFLNWATPLDAGTIGKVSAGIVMDD
                     HFIPKSSDLDLARSLIGPAFAIALLGAIESLLCGVVAGRMTGKKLAVNQELIAQGVGN
                     IVLPFVGGVPATAAIARTSVGVKAGGTTRLVAIIHSVVLLVGGIFFADLIKHIPVAAL
                     SGVLFVTAWRMNEWHAIHFYREHRLKGAIATFFVTMLATVEFNLSEAIAAGVLVSLAL
                     FLAQVARLHVNVEPVDWEKIGVPPLPASDVQVIYVSGPLFFGSVNQFVERVEELPFAQ
                     TLILSMRGVPMADVSSVQAIDHLWQTHRKQGGMVFVTGLQPQVRRMMERSGLIAAMGD
                     GQFLWSADQALRVVAGHPAIASRPATITAVDDGLGDLPLGVLPIG"
BASE COUNT         1019 a         1817 c         1621 g          789 t
ORIGIN      
        1 gcgcgcgatc gctccaggcg agaatgtcct ggtgggccgc cgaacgaccc gtaaaggtga
       61 gtcgcaggct gatcgcttcc agagacccat cactctggct cgaaaacgtc gggtcccgca
      121 tcgcggaact cagcgcacga agaaatcctt cctcgtcgac aacatcggca aggtcgacct
      181 cccgcgattc gtagcgaact ggcgaaagag ggatcggccg caacgcaacg ggctcgcccg
      241 gctggaaggt tgcgagccac accccgtgag ttcccggttc tccgggatcg agcgcatacg
      301 gcgatccggg atagatggca gtgccgcggc cttgcggccc ggtcaacacg cgcgccgcgt
      361 gaatgtgacc gagcagccag aaatcgaccg gatagcgcca gagatcgaca ctccgaactg
      421 gtgcgtggtt gctgaccatc gcatcgagat cggtgtggaa cagtccgagg aggggaacgc
      481 catcaccacc ctggggccgg taatgagcaa gcgggctctc cgggacgcga ccacggggaa
      541 aggaccaacc atcgacgaag agaacgggca cgttatcacg tcgcagcgtg aaccgctccc
      601 aggcgccgta gcgtcccagg agatgaaagc gctcgccacc gctggcgccg atcagacgcg
      661 gcaggacatc ccagtcgtga ttaccggaga cggccaccgt atcgatgcca gcaccgtcga
      721 gccggcggat tccagcctcc aggggaccaa gtgcctcgta ggacttgctc tcctcgtcga
      781 cgatatcgcc gctcaatgcg accagatcga cccgctctgc gatcgcgata tcgactgccc
      841 gcgaccaggc gtcgaccgag cggtaggtcc cggaaactct gctggcgcga cgtcccatat
      901 ggacgtcacc caggcacaag acgcgaaccg acatcgctgc tcctcgtgca tgattacgtt
      961 acggtaaaag gtgtccctta ccccgcatca tacaagagaa gcacgcacgc gtcgatagcg
     1021 attccagggc cgttcaccgg cgagcgagtc gactcagggc ggcgctcgcg atcacgagca
     1081 gcgaggcgag gatcgtcccg accgagagat gttcgccgag caacagcgcc gcccagatca
     1141 ggctcagaac cggctgcacc aattgcagtt gcccgacttt cgcaacgccg ccgaccgcca
     1201 ggccccggta ccagggaaag aagccgaaga acatgctgac cgcgccgagg tagagcatgc
     1261 tccaccaggc ttcggcatcg ccgctcggcc agccgttcca gacgtagagc acgaccgaga
     1321 gcatgacgat caccgggagg ctgaagacga gcgcccacga gatcacgcgc cacccgccca
     1381 gttccctggt cagccgcgca ccctccgcat aaccgagact cgccatcacg acggcaccaa
     1441 ggagcaagag gtcgccccac tgcggatgcc cggctccctg cacgatcgcg aaaacgacca
     1501 ccgccgcgac accgagcgag caggcgagcc agaagctccg cggcggatgt tcccccgcgc
     1561 gtagcaccgc catcacggcc gtcgccgccg gcagcaatcc gatcacgacg actccatgcg
     1621 ccgccgggag atattgcatc gcgagcgacg agagcggccc gaagccaagt acggtcccca
     1681 acgcgacgat gcagatcggc caccagtgcc gccgctccgg ccacgcttct ctccgccaga
     1741 gcaggatgac gatcgcgagg gccgaagcga gcagtgcccg ctcgatcccc acgaagaggc
     1801 cgccgagctg gggcgaggcc acccggctga acggcagcgt gaagctgaag gcgaggatac
     1861 ccagcatccc gaagacgaat ccctggccct cgttcgagcc gagccagagg ccgcgatcac
     1921 tcgccagcac gtccgtctgc tccgtcgaat ccgctgtcag aggtgccgtt tccgcgaccg
     1981 aatccatcga gatgctttca atacagggcg aatcgcatca gatcggaccc tcatgatcgc
     2041 cgggatcggg atcgcgaaca acccgggccg cggtgcgcgc gtgcgctcac gcgaggacgg
     2101 tctcgccgca ggcatcacgc agcgccccga cgagtgggtt ccagggacgc gacatcgggc
     2161 ccgtgaagac gagtgtccgg tccgtcaccg gcaccaacgg caccatcacg aggtcccgcg
     2221 gcaacatcgc ttcgccaagc gagggcatga tcgagatccc gacgtgaccg acgatcatac
     2281 tcagcaacgt gccaacttca cgcacccgct gcccgagcac catcgactca tgcgcctggg
     2341 caaagatccg ggagatctgc gcctcgcaac cgccggtcga gatgatgaag gggtcgtcaa
     2401 gaagatcgtg aagcgtcacc gcgacctcgt tcgccagggg atgatcgcgc cggacaaccg
     2461 cctggaaagt atcccggcca acgatgacac cgcgatcagg catcggatcg gggtcgatca
     2521 ggatcgccgc gtcgaccatc ccaccctcga gccagagcgc cagttcatcg tcgtcgccct
     2581 cgaagatctg gacctcgacc cggggatgcg tggatcgcca gcgcatcaac agctccggca
     2641 tcaacccctg acagacggtc ggcgccgcgg cgaggcggac aagaccggct gtgagcccga
     2701 gatgctcccg cacggtcgcc tgcaccgctt cgaccgacgc caacgccgcc cgcgcgtgag
     2761 gcaggatctt ctcgccgagc ggcgtcggtt gcagctgcgg cttccgctgg aggacgggcg
     2821 ccccgatcgc cttctcgagt gtcgcgatcg cgtgggaaac ggcgggctgc gtcatcgcga
     2881 gacactcggc cgcctctccg aacccacccc cgtcgatcac cgccgcgaat gcacgaagct
     2941 ggactacggt cagattcata actaaccctc atgtcgcgca aaggaaacgt catttgcctt
     3001 atcgtacccg gtctcgctag cctgtgttgc acgcggtcgg tttcgatcgc aagaaacgtc
     3061 gtgtcgccgc tcgcgacgat cggcgatgat ccgggacagg ttgatcgacg ggaagcatcg
     3121 gggaagcaga tgatgaaaga aaccgcagtg agcggcaggc gagggacact gcggaaggcg
     3181 gaaaattccg ttctggtccc gttcgtcctg ctcgccgtca tttggggagc gagctttctc
     3241 ttcatcaagg aagcgctgaa cgggttcgcc ccggttcagg tcgcggcggg acggatcagc
     3301 ctcggcgcga tcacgctggt cgtgatgatg atggtgacgc gtcgaaagtg gccgcggggc
     3361 cgcatctgga tccacctcgt cgtgggggcg atcccgctct gcgtcctccc gatgttgctc
     3421 tactcctggg cgacccagca cctgccgtcc agtctcgaga gcgtctacaa cgcgacgacg
     3481 ccactgatga cgatgctgat cgccgtgctc ctcccgtccg ccgagagact ctcccgtgcc
     3541 cggctgacag gtctcctcgt cggcggcgcg ggcgtcctga tcgtgctcgc gccctggcac
     3601 ctgatcgccg acaccggcgc gttggcagga accggctgag cgcagctcgc ctgtctcgcc
     3661 gcgacattct gctacggcgt cgccttcaac tacacgcgtc gattcatctc gccccaccac
     3721 ctcgatccgc tttcgatcgc cgcagcccag atcggcagcg gcgccctgat gatcctcgcc
     3781 atcgtcccct tcttcggaac ctctgcggcg catctcgacc tgcggatcgc tgcctcgctc
     3841 ctggcactcg gtgtgctcgg caccggcttc gcctatgtct ggaacaacgc cgtgatcgcc
     3901 ggctggggcg cgaccaacgc ctcgacggtc acctacgtga tcccgatcat cggtctcgcg
     3961 ctcggcatcg tcgtcctcgg ggaatcgctc acctggaatc tccccgtcgg tggcgcggtc
     4021 acgatcgccg gtatcctgat cagccagggc cgacttcgcc ttccccaccg gtcgacgatc
     4081 acgggcaagc gcgccgaatc gtccgcctag ccgatcggca gcacgcccag cggaagatcg
     4141 ccgagcccat cgtcgacggc agtgatcgtg gccggtcgcg aggcgatcgc gggatgtccc
     4201 gccaccacgc gcagcgcctg gtcggcactc cagaggaact gcccgtcacc catcgcggcg
     4261 atcaggccgg agcgctccat catccgccgc acctgcggct gcagtccggt cacgaagacc
     4321 atgccaccct gcttccggtg ggtctgccag aggtgatcga tcgcctgcac gctggagaca
     4381 tcggccatcg gcacgccacg catcgagaga atcagcgtct gcgcgaacgg gagttcctcg
     4441 actcgctcga cgaactggtt caccgaaccg aagaagagcg gcccgctgac atagatcacc
     4501 tggacatcgg acgcgggcag cggcggaacg ccgatcttct cccagtcgac cggctcgaca
     4561 ttcacgtgca atcgcgcgac ctgggcgagg aagagcgcca ggctgacgag cacaccggcg
     4621 gcgatcgcct ccgagaggtt gaactccacc gtcgccagca tcgtcacgaa gaaggtcgcg
     4681 atcgccccct tcagccggtg ctcacggtag aagtgaatcg cgtgccactc gttcatccgc
     4741 cacgcggtga cgaagaggac cccggaaagc gccgcgaccg gaatgtgctt gatcagatcg
     4801 gcaaagaaga taccgccgac aaggagcacg accgagtgaa tgatcgcgac gagccgcgtc
     4861 gtgccgccgg ccttgacgcc gacgctggtc cgcgcgatcg ccgccgttgc cgggacgccg
     4921 ccgacaaacg ggagcacgat gttcccgacg ccttgcgcaa tcagttcctg gttgaccgcc
     4981 agtttcttgc ccgtcatccg gccggcgacg acgccacaga gcaggctctc gatcgcccca
     5041 agcaatgcga tcgcgaaggc cgggccgatc agactccgcg cgaggtcgag atcggaggac
     5101 ttcgggatga agtgatcgtc catcacgatc cctgccgaaa ccttgccgat cgttccggca
     5161 tcgagcggcg tcgcccagtt gaggaatgtc gccaccacga tcccgatcag cgcgatggga
     5221 atgcccgggg ccttcaggaa cctggg
//
LOCUS       DASTMD010000299         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1901777, whole genome shotgun sequence.
ACCESSION   DASTMD010000299 DASTMD010000000
VERSION     DASTMD010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901777"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1609)
                     /gene="mqo"
                     /locus_tag="VFQ54_04415"
     CDS             complement(98..1609)
                     /gene="mqo"
                     /locus_tag="VFQ54_04415"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF003605.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HEU0164264.1"
                     /translation="MKRVDTFAEPDVVLIGGGIMSATLGVLLREVRPDWSITLYERLK
                     DVATESSDPWSNAGTGHAALCELNYTPEKADGSIDISKALQINEQFHQSRQLWASLIE
                     RGELPNPTTFINPIPHMSYVKGDADVSFLRRRYDALSREPLFAGLEYTEDRERIAEWA
                     PLMMRGRDSSAPMAFTRHEGGTDVNFGELTRQLVASLGRSGGEVATNHDVTGLDKQPD
                     GRWKVHVKDRSSGERTTVTARFVFVGAGGRAIHLLQQSGISEAKGYGGFPVSGQFIRS
                     TNPALIAGHHAKVYGKSQQKAPPMAMPHLDTRVIDGKEGLLFGPYAGFSPKFLKQGSI
                     FDLLRSVKPDNLLTMLNVAKNEFALTRYLIGQIMQSNNARIETLRDFLPGADGGDWEL
                     IHAGMRVQTMKRTAKSRGALEFGTEVVSAKDGTIAGLLGASPGASTAVSIMLDVIQRC
                     FPGEYPEWEPKLSGLIPSIGRKLNEDPVLLGEVQAMTDRTLQLGPASHVVGAD"
BASE COUNT          291 a          577 c          517 g          324 t
ORIGIN      
        1 ccgacggact ggatggcgaa gctccacggc tggcccggga tggatgctga cgagctccgc
       61 cgaatatccc tgcgggtcgc gggcgtcgcg tcctgaacta gtcggcgcca accacatgac
      121 tggccggtcc gagttgcagt gtccgatcgg tcatcgcctg gacctccccg agcaggaccg
      181 gatcctcgtt cagcttccgg ccgatcgacg ggatgagccc actgagcttc ggctcccatt
      241 ccggatactc acctgggaag cagcgctgaa tgacatcgag catgatcgaa accgccgtcg
      301 atgcacccgg cgacgccccg aggagtccgg caatcgtacc gtccttcgca ctgacgacct
      361 cggtgccgaa ctcgagggcg ccccggctct tcgccgtgcg cttcatcgtc tgcacgcgca
      421 tcccggcgtg gatcagttcc cagtcgccgc catccgcccc gggcaggaaa tcccgcagcg
      481 tctcgatccg ggcgttgttc gactgcatga tctggccgat gaggtacctc gtgagcgcga
      541 attcattctt cgccacattc aacatcgtca acaggttgtc cggcttcacc gatcgcaaca
      601 ggtcgaagat cgacccctgc ttgagaaact tcggcgagaa gccggcgtac gggccgaaga
      661 gcagaccttc cttgccgtcg atcacccggg tgtcgaggtg gggcatcgcc atcggcggcg
      721 ccttctgctg cgacttgcca tagaccttgg cgtgatgacc cgcgatcagc gccgggttcg
      781 tgctccggat gaactgaccg ctgaccggga atccgccata ccccttcgcc tcggagatcc
      841 ccgactgctg caggagatga atcgctcgtc caccggcgcc aacaaagacg aagcgtgccg
      901 tcaccgtcgt tcgctctccc gaggatcgat ccttgacgtg gaccttccag cgaccgtcgg
      961 gctgcttgtc gagaccggtg acgtcatgat tcgtcgcgac ctcaccgccg ctgcgaccca
     1021 gcgacgcgac gagttgccgt gtcagttcgc cgaagttgac gtcggtgcct ccctcgtgcc
     1081 gggtgaatgc catcggcgcc gagctgtccc ggccacgcat catcagtggc gcccactccg
     1141 cgatccgctc ccggtcctcc gtgtactcga gcccggcgaa gagcggctcc cgcgagaggg
     1201 cgtcgtaccg ccggcgaagg aaggagacgt ccgcgtctcc cttcacgtag ctcatatgcg
     1261 gtatcgggtt gatgaaggtc gtgggattcg gcagctcgcc acgctcgatc agactggccc
     1321 agagctggcg tgactgatga aactgctcgt tgatctgcag cgctttcgag atatcgatcg
     1381 agccatccgc cttctccggc gtgtagttca gttcgcacag ggcggcatga ccggtacccg
     1441 cgttgctcca cggatcggag ctctccgtcg ccacgtcctt caggcgctcg tagagcgtga
     1501 tcgaccaatc cggccgaact tcccgcagaa gtacgccgag tgtcgcgctc atgattccgc
     1561 cgccgatgag gacgacgtcc ggttctgcaa aagtgtcaac gcgcttcatg gatacctgcc
     1621 gggccggata aagggctggc ccgtgccttg caacccccgc ctgagcttct ttcgcgccaa
     1681 ttttgcgcaa gcgggcgtta aagcgctgg
//
LOCUS       DASTMD010000300         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1098844, whole genome shotgun sequence.
ACCESSION   DASTMD010000300 DASTMD010000000
VERSION     DASTMD010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098844"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1294
                     /gene="hrcA"
                     /locus_tag="VFQ54_04420"
     CDS             248..1294
                     /gene="hrcA"
                     /locus_tag="VFQ54_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU0164265.1"
                     /translation="MERLSDGSPRLTERQRAILKLIVQEYVSSGRPVGSRTLTERYPV
                     GVSSATIRNEMVNLEEVGMVQQPHTSGGRVPTDQGYRYYVHHLMGAPELSSGDQIMIR
                     HQFRQVEFQLDQWVDFAAAVLAETAGNVSIVSAPRTVTARLRHFELISLQPRLAMLIL
                     VTQESAVRQMMLHLPEDTEQSVLRGLSDDLVPQLRGKSAAEIADLVVGTSPLARYVLD
                     QIGLGLEGLDSVERTQIRHSGIENILGQPEFAGGAAHSVLELLRGGSLLGAVLPQLSV
                     SGDVQVFIGDENPAGALRQFGVVVATYGIDGEATGILGVLGPTRMSYSRSISSVRYMA
                     RLMSDLMEDLYSSA"
     gene            1392..1979
                     /locus_tag="VFQ54_04425"
     CDS             1392..1979
                     /locus_tag="VFQ54_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU0164266.1"
                     /translation="MAEVNDDNKVYEGWPDPKTVGKDADATTGTSDDVATESVEAEVD
                     ELAAITAERDEYLDQLQRSRAEFANYRRRIEQQQQALRDIVQRDALAQFLPVIDDFDR
                     GLAAIPESERGSSWVAGITMIQTKLAAILERAGVTVIDALNQPFDPALHEAVATEPGS
                     KGEFVVEVYQKGYRVGDQLARPAMVKTGDAPSPSA"
BASE COUNT          376 a          625 c          660 g          349 t
ORIGIN      
        1 tggggcgttc gagcagggcg gaggttggcg acccgacgaa cgggacgacg gccaacggac
       61 accttgcgaa cggggctcat ccggacaccg aacagggccg ctgacggctg ggattcccgg
      121 cgacagggcg gtctccgcga ggctcccggc agggtcccaa ccttgacaca aaggggtcaa
      181 tcctgctatc ctccttgtgt tagcactcgt tgagtgagag tgctaatcca ctcaggagcg
      241 agatatcgtg gagcgattat ccgatggatc tccccggctg accgaacggc agcgggccat
      301 cctcaaactt atcgtccagg agtacgtcag ctcagggcgc ccggtggggt cgaggacctt
      361 gaccgagcga tacccggttg gcgtttcgtc ggcgacgatt cgaaacgaga tggtgaatct
      421 tgaggaagtc gggatggtcc agcaaccgca cacctcgggt gggcgggttc cgaccgatca
      481 gggctatcgc tactacgtgc atcatttgat gggtgcgccg gagctctcgt caggcgatca
      541 gatcatgatc cgccaccagt tccggcaggt cgagtttcag ctcgaccaat gggtcgattt
      601 cgccgccgcg gtactcgcgg agacagcagg caacgtctcg atcgtctccg ccccacgcac
      661 ggtgaccgcc cgtctccgtc atttcgaact gatctccctc caaccacgac ttgcgatgct
      721 catcctggtg acccaggaga gtgcggtgcg gcagatgatg ttgcatctcc cggaagatac
      781 ggagcaatcg gtgcttcgcg gtctctcgga cgacctggtt ccgcagctgc gtggcaagag
      841 tgccgccgag atcgccgacc tcgttgtcgg tacctcgcca ctcgcccgct acgtgctcga
      901 tcagattggc ctgggactcg aggggctcga ctccgtggag cggacgcaga tccgtcattc
      961 cggaatcgag aacatccttg gccagccgga attcgccggg ggagcggcac acagcgtgct
     1021 cgagctcctg cgcggcggat cgttgctcgg ggccgtactt ccccagctct ccgtgagcgg
     1081 ggacgtccag gtcttcatcg gcgacgagaa cccggccggt gccctccggc agttcggcgt
     1141 ggtggtggcg acgtacggga tcgatggcga ggcgaccggt attctcggtg tcctcggtcc
     1201 gacccggatg tcgtactcgc ggtcgatctc ctcggtccga tacatggccc gattgatgag
     1261 cgacctcatg gaagatctct attcatcggc ctaagtctgg cgaggacacg ccgggacgcg
     1321 gttgcttcga atgaagtgac tcatccacga tgtgtgcccc gcgccgcaat tacggatatc
     1381 gggagaagcg agtggcagag gtaaacgacg acaacaaggt gtacgaggga tggcccgatc
     1441 cgaagacggt cgggaaggat gccgacgcca ccaccgggac cagtgacgat gtagccaccg
     1501 agagcgtcga agccgaggtg gatgaactcg ccgccatcac cgccgagcgt gacgaatatc
     1561 tggaccagct gcagcgcagc cgggccgagt tcgccaacta ccggcgtcgt atcgaacagc
     1621 agcagcaggc gctgcgggac atcgtccagc gcgacgcgct cgcacagttc ctgccggtga
     1681 tcgacgattt cgatcgcggt ctcgcggcaa ttccggagtc cgagcgcggc agcagctggg
     1741 tcgccgggat cacaatgatc cagacgaagc tggccgccat cctcgagcga gccggtgtca
     1801 cggtgatcga cgcgctcaac cagccgttcg atcccgccct gcatgaggcg gtcgcgaccg
     1861 agcccggatc caagggcgag ttcgtggtcg aggtctatca gaagggctat cgcgtcggcg
     1921 accagctggc gcgcccggcg atggtcaaga ccggcgacgc accgtcgccc agcgcgtaga
     1981 tacccggaca ccccgggaga cctcatccgg
//
LOCUS       DASTMD010000301         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1187884, whole genome shotgun sequence.
ACCESSION   DASTMD010000301 DASTMD010000000
VERSION     DASTMD010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187884"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VFQ54_04430"
     CDS             complement(<1..614)
                     /locus_tag="VFQ54_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0164267.1"
                     /translation="MTDPVFAISSHLDIQEVSVQSLGSGSSGNAFLVRRGHRTLLVDC
                     GVGIRTIQREMRAFGGNPAQLDGILLTHEHIDHVRTLPKVLDAGMPVIATNGTAGMAD
                     IAPDQHLAIDGDAPIEIAGFTIWAFGVRHDAVQPCGYLIETPETRITILTDLGSWQEH
                     LAEPIAASDLIVLEANHDEEMLRRGPYPMHLKRRVASEIGHLSNR"
     gene            874..>3079
                     /locus_tag="VFQ54_04435"
     CDS             874..>3079
                     /locus_tag="VFQ54_04435"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEU0164268.1"
                     /translation="MIVALVVVLAIIILLFAFSPFWINWLWFGSVGYRSILVTNYLNS
                     TIFFIVGGLIAAAIFVLNVSFALRQTRDARLAREGIVGRISQRVIQFLAYGGGIVIFI
                     LAGITSASDWKATMLAIRGGDFGVKDPTFHMDAGFYIFRLPVLHDLHGYLLRLGFTTL
                     IAVAVVYGIRLGVRFRRWGDVPWEALRHLSAIGCVLLLLIAASYLLDNYDLVYSTRGV
                     VMGPGFTDVNIVRPLNWLMAILSAGAGIALLFGVVLRTPKWLIGLLGGWALLAFIVTP
                     LLPIGVQRLIVDPNEFKREEKYIERNIEMTRAGFGLDTVATQNVTGQDPITAAQLPTN
                     QPPLSNVRIWDYRVVQPIYQQLQTFVPYYTFGDIDIDHYTINGQQTEVLISARELNTD
                     GLPSNSQTWTNLHLAYTHGYGAVVSPVSQSSSDGWPQFLVSNIPPAGPQDLKITQPEI
                     YYGEGSQDWIVVHTDQAEISGIAEDNAPSSTTAYTGKAVGSISLGNPVTRLLASLAYG
                     DRNLFISSQLTGDSRLIVDRSIVDRAQKIAPFLTYDQDPYLVIANGKLYWVMDAYTSS
                     SAFPGAARFDGDNYLRNSVKVVVDAYDGTTTFYRTDEPDPIADAYGKIYGDLFTPISQ
                     APAAISEHFRYPEQEFTTQSEVWSTYHADTARSFYDGDDKWTIAQEQVNGAMQSVEPY
                     YVTLPLPGQTTSSFALTAPFTPGGNQNRQNMTAWFAGTADATGKTTLQLYRYP"
BASE COUNT          534 a         1038 c          935 g          572 t
ORIGIN      
        1 cggttcgaga ggtgtccgat ctcggacgcg acccgccgct tgagatgcat cgggtagggg
       61 ccacgacgca acatctcctc gtcgtgattc gcttcgagca cgatcaggtc gctcgccgcg
      121 atcggctcgg cgagatgctc ctgccacgac ccgagatcgg tgagaatcgt gatccgcgtc
      181 tccggtgtct cgatcaggta tccgcagggc tgaaccgcat cgtgccgcac gccgaacgcc
      241 cagatcgtga aacccgcgat ctcgatgggt gcgtcgccat cgatcgcgag atgctggtcg
      301 ggcgcgatat ccgccatccc ggccgtaccg ttcgtcgcga tgaccggcat cccggcgtca
      361 agcaccttcg gcagtgtccg tacgtgatcg atgtgctcgt gggtcaggag gataccgtcg
      421 agctgggccg gattcccgcc gaacgcccgc atctcgcgct ggatcgtgcg aatcccgacg
      481 ccacaatcga cgagcagcgt ccggtgcccg cgccgcacga ggaaggcgtt ccccgagcta
      541 ccgctcccga gactctggac cgagacttcc tggatgtcga ggtgggacga aatggcgaaa
      601 acggggtcgg tcatgaggcg ggtcgtccaa tcatcgcgac gctgcgatcg ttcgcggggt
      661 gtcccctccg gcaggagtga tgcccgccat cgtaccatag agcgataggg cagccgaccg
      721 ggtggaccca gccgcttcac acggagcgac tggtggctgc attcacgggg tggggagatg
      781 gtctttcaac gaagagccgg gggaaacacc tccgggcagc gccgtatccg gccgagcttg
      841 ccacgtccac acatctccgc gtcgacacga cggatgatcg tggccctggt cgtcgtcctg
      901 gcgatcatca tcctgctctt tgcgttctct ccgttctgga tcaactggct ctggttcggc
      961 agcgtcgggt accgctcgat cctggtcacg aactacctga actcgacgat cttcttcatc
     1021 gtcggcgggc tgatcgccgc cgccatcttt gtcctgaacg tttcctttgc gctccgccag
     1081 acgcgggacg cccgcctggc tcgcgagggg atcgtcgggc ggatcagcca acgtgtgatc
     1141 cagttcctcg cctacggcgg cgggatcgtc atcttcatcc tcgccggcat cacctcggcg
     1201 agcgactgga aggcgacgat gctggcgatc cggggcggcg acttcggcgt caaggatccc
     1261 accttccata tggatgccgg gttctatatc ttccgccttc ccgtgcttca cgatctccac
     1321 ggctacctgc tgcggctcgg cttcacgacg ctgatcgcgg tcgccgtggt ctacggcatc
     1381 cggctcggcg tgcgctttcg ccgctggggt gacgtgccgt gggaggcgct ccgccatctg
     1441 agcgcgatcg gctgcgttct ccttctgctg atcgccgcga gctacctgct cgataactac
     1501 gatctcgtct actcgacgcg cggcgtcgtg atgggtccgg gtttcacgga cgtcaacatc
     1561 gtgcggccgc tgaactggct gatggcgatc ctttccgccg gagccgggat tgcgttgctc
     1621 ttcggcgtcg ttcttcgcac gccgaagtgg ttgatcggct tgctcggtgg ctgggcgctg
     1681 cttgcgttca tcgtcacacc gctcctcccg atcggcgtcc agcgcctgat cgtggatccg
     1741 aacgaattca agcgcgagga aaagtacatc gagcgaaaca tcgagatgac gcgggccggc
     1801 ttcgggctcg acaccgtcgc gacccagaac gtgaccgggc aggatccgat cacggccgcc
     1861 cagttgccga ccaaccagcc tccgctgagc aacgtccgga tctgggacta ccgcgttgtc
     1921 cagccgatct accagcagct gcagaccttc gtgccctact acaccttcgg tgacatcgat
     1981 atcgaccact acacgatcaa cggccagcaa accgaggtcc tgatctccgc ccgggaattg
     2041 aacacggatg gcttgccatc caattcccag acctggacga acctgcacct tgcctatacg
     2101 catggctatg gcgcggttgt cagcccggtg agccagtcat cgagcgacgg ctggccccag
     2161 ttcctcgtca gcaacatccc gccggccggc ccccaggatc tcaagatcac gcagccggag
     2221 atctactacg gcgaggggtc gcaggactgg atcgtcgtcc acaccgacca ggccgagatc
     2281 tcggggatcg cggaggacaa cgcgccgagt tcgacgaccg cgtacaccgg aaaggccgtc
     2341 ggttcgatca gtctcggcaa tccggtgacg cgtctcctcg catcgctggc gtacggagat
     2401 cgcaacctct tcatcagcag ccagctcacc ggtgattccc ggctgatcgt cgaccgctcg
     2461 atcgtcgatc gcgcgcagaa gatcgcgccg ttcctgacct acgatcagga tccctatctc
     2521 gtgatcgcga atggcaagct ctactgggtg atggacgcct acacctcgtc gtcggccttc
     2581 cccggcgcgg cgcggttcga tggcgacaac tatctccgca acagcgtgaa ggtcgtcgtc
     2641 gacgcatacg acggcacgac gaccttctac cggacggacg aaccggatcc gatcgcggac
     2701 gcctacggca agatctacgg cgatctcttc acgccgatct cgcaggcgcc ggcggcgatc
     2761 tccgagcact tccgctaccc ggagcaggag ttcactacgc agtccgaggt ctggtcgacg
     2821 taccacgccg acaccgcgcg ctccttctac gacggcgatg acaaatggac gatcgcccag
     2881 gagcaggtga acggcgccat gcagtcggtc gagccctact acgtcacgct ccccctgccc
     2941 ggccagacga catcctcgtt tgcattgacg gcgccgttca cgccaggagg caatcagaac
     3001 cggcagaaca tgacggcctg gttcgccgga acggccgacg ccaccggcaa gacaacgctc
     3061 cagctctacc ggtatccgc
//
LOCUS       DASTMD010000302         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1901862, whole genome shotgun sequence.
ACCESSION   DASTMD010000302 DASTMD010000000
VERSION     DASTMD010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901862"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VFQ54_04440"
     CDS             <1..702
                     /locus_tag="VFQ54_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU0164269.1"
                     /translation="YGFKRQTNGGMAKFNRIDANSILHRIPDALSESEAAYIEPMACA
                     WHAVDRGEIKEGDTVVIGGVGNIGLCMLQIAKLRKPGKLIALDTKPYRLEKALQFGAD
                     VAIDVTRGDAVQQVLDLTDGYGCDVYIEASGNPAGVQQGLQMIRKLGTFVEFSVFNDP
                     TTVNWTVIGDTKELNIHGSHLGPGGYAPSIEALVSGTVDVKPLLAEAHPLTDFDIAMQ
                     QALSGDVLKTIIVPV"
     gene            712..1143
                     /locus_tag="VFQ54_04445"
     CDS             712..1143
                     /locus_tag="VFQ54_04445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU0164270.1"
                     /translation="MTDIRILSLPDGRDEELAALWEACGLVASYNPPLEDIAFARQSL
                     DAEILVGIRDDRIVASTLVGHDGHRGWLYYVAVSPDQQGTGLGAKIVAAAEEWLREKG
                     VRKVQLLVRPTNTKVLSFYEHLGYETSPVTVMQRWLIDDPR"
     gene            1383..2918
                     /locus_tag="VFQ54_04450"
     CDS             1383..2918
                     /locus_tag="VFQ54_04450"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEU0164271.1"
                     /translation="MPGSLLLGVDIGTSSSKGVLCTPEGDVLASHAIEHETSFPRPGW
                     AEHDADAIWWGEFVRICRVLLSGKYSGDDVGGVAVSAIGPCMLPVDAGDRPLRPGILY
                     GIDSRAQEEIAWLESRYGADAMFDLNGMALTSQAVGPKIRWLREHEPELFAKTARIHS
                     ASDYLVLRLTGEHVIDRHSASYFVPLFDVRKLDWDDRFADEVIGIDRLPHLLDADEIA
                     GTVSARAAAGTGLAIGTPVTTGTIDAAAEAISVGVTAPGDMMVIYGSTLFLIDLVAEP
                     HPDRRMWTAAYCLPGVHAVTGSITSSGLLTKWFRDQLGGAERAGGANAYQALAALAAA
                     TPPGADGLICLPYFAGERTPLNDPDARGVFAGLTLRHTRGHLYRAVLEAIGYGLRTNL
                     EVMAEMNAAPKRLVAVGGGTQSDVWTQIVSDVTGMAQILPEKTVGASYGDALLAGLAT
                     GIVDRATGIDTWVRTKRVVAPNAANTAIHDRGYDIYRNLYLHARDDLHALAQLDTAFD
                     RPA"
     gene            3011..3247
                     /locus_tag="VFQ54_04455"
     CDS             3011..3247
                     /locus_tag="VFQ54_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEU0164272.1"
                     /translation="MTAITDPANIEHVTLSTPDIGCDHCVATIQETLSVLDGISSARA
                     STETKSVDVVFDAEVISLGKIEAALEEVGYPVTR"
     gene            3605..>4635
                     /locus_tag="VFQ54_04460"
     CDS             3605..>4635
                     /locus_tag="VFQ54_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU0164273.1"
                     /translation="MPSTSGMHLVSSEDNADALQRKQEVVERILSTHPLLVASNRGPV
                     TFSPRQDGSFSARQGSGGLVTAVRAVLKDHQAIWIAAAMNEGDRSRAQVAVDDGEDFI
                     TDSPDSLFKLRFVTPSPDAYHQYYNVISNPLLWFLQHYLWDTPRSPDITHETWRAWRE
                     GYVAVNQQFADEVLVACERTEAEPIIMLQDYQLYLVGKMIREKRPQAIIQHFIHIPWP
                     DPDYWRLLPMEMRRSICQGLLACNIVGFQTPENARSFMYTCEAYVPGCEIDYIERTVT
                     LDGHVTEVRAYPISIDVESVRQTAYSQEARAYERYLPSHWNEYTILRIDRAEPSKNIV
                     RGFAAFDRFL"
BASE COUNT          862 a         1546 c         1421 g          806 t
ORIGIN      
        1 tacggtttca agcgacagac caacggcggg atggcgaagt tcaaccggat cgacgccaac
       61 tcgatcctcc acaggatccc ggatgcgctc tccgagagcg aagccgccta catcgaaccg
      121 atggcctgcg cctggcacgc cgtcgatcgt ggggagatca aggaaggcga caccgtggtc
      181 atcggcggcg tcgggaacat cggtctctgc atgctccaga tcgcgaagct caggaaaccc
      241 ggcaagctga tcgcactcga taccaaaccg tatcgccttg agaaggcgct gcagttcggg
      301 gccgatgtcg cgatcgatgt cacgcggggt gacgccgtcc agcaggtgct cgatctcacc
      361 gacggctacg gctgcgatgt ctacatcgag gcgagcggga atccggcagg ggtccagcag
      421 ggactccaga tgatccgcaa gctcggcacc ttcgtcgaat tcagcgtctt caatgatccc
      481 acgacggtga actggacggt gatcggcgat acgaaggaac tcaacatcca cggcagccat
      541 ctcggtccgg gcggctacgc gccgtcgatc gaggcgctcg tcagcggaac cgtcgacgtg
      601 aagccgctgc tggcggaagc gcacccactg accgacttcg acatcgcgat gcagcaagcc
      661 ttgagtggtg acgtcctgaa gacgatcatc gtgccggtct aggagcgctc gatgaccgac
      721 attcggatcc tttcccttcc cgacggccgc gacgaggaac tcgccgcgct ctgggaggcg
      781 tgtggactcg tcgcttccta caacccgcca ctggaggaca tcgccttcgc tcgccagagc
      841 ctcgatgcgg agattctcgt tggaatccgg gacgaccgga tcgtcgcctc cacgctcgtc
      901 ggtcacgacg gacatcgcgg ctggctgtac tacgtcgccg tatctccgga ccagcaggga
      961 acgggactcg gtgcgaagat cgtcgccgcg gcggaggaat ggctgcgcga aaaaggcgtt
     1021 cgcaaggtcc agctcctggt tcgaccgacg aacacgaagg tgctttcctt ctacgagcat
     1081 ctcgggtacg agacatctcc ggtgacggtc atgcagcggt ggctgatcga cgatcctcgt
     1141 tgagtgaggc gacgctcacg ccagcgtgaa cacggcggcg gtgtgtccac gactcggtcc
     1201 gggggacccg gtcgtacgta gggcgggacc gccgctgccg gaccgcaggg ccggacggcc
     1261 tgtcccgcct gtccttcccg aggaccgtgc tcccggtaca ggcccgaagg tcgcccaggt
     1321 catcgcaact gtgatcgtca gaatgaagat cgacttcgct gtcgaggaag aaaggctcgc
     1381 tcatgccagg ttcgttactg ctcggcgtcg acatcgggac atcaagctcg aagggtgtcc
     1441 tctgcacgcc ggagggtgac gttctcgcgt cccacgcgat cgagcatgag acgtcgtttc
     1501 cccgtcccgg ctgggcggag cacgacgccg acgcgatctg gtggggcgag ttcgtccgga
     1561 tctgtcgcgt gttgctctcc gggaagtact cgggcgacga cgtcggcggc gtcgccgtca
     1621 gcgcgatcgg accatgcatg ttgcccgtcg acgccggcga tcgcccgctt cgtccgggca
     1681 tcctttacgg gatcgacagc cgtgcccagg aagagatcgc ctggctggag tcccgctacg
     1741 gcgccgacgc catgttcgac ctgaacggga tggcgctcac ctcgcaggcg gtcgggccga
     1801 agattcgctg gctgcgcgaa cacgagccgg agctcttcgc aaagaccgca cggatacact
     1861 ccgcctccga ttacctggtc ctccggttga ccggcgagca tgtgatcgat cgtcattccg
     1921 cctcgtactt cgttccgctc ttcgatgtcc gcaagctcga ctgggacgac cgcttcgcag
     1981 acgaggtgat cggcatcgac cgcctgcccc acctgctcga tgccgacgag atcgcaggaa
     2041 cagtctccgc tcgggcggct gccgggacgg gcctcgcgat cggaacgccc gtcaccacgg
     2101 gaacgatcga cgctgccgcc gaggcgatca gcgtaggggt cacggcgccc ggcgacatga
     2161 tggtgatata tggaagcacg cttttcctga tcgatctcgt cgccgagccg catcccgatc
     2221 gccggatgtg gaccgcggcg tactgcctgc ccggcgttca cgccgtcacc ggcagcatta
     2281 catcgtccgg actcctcacg aaatggtttc gggatcagtt gggcggcgcc gagcgagcgg
     2341 gcggagcgaa cgcataccag gcgctcgcgg cgcttgccgc ggcgacacct cccggcgccg
     2401 atggcctgat ctgcctcccc tacttcgcgg gcgagcgcac cccactcaac gatccggacg
     2461 cgcgcggcgt cttcgccggt ctcacgctcc gtcacacccg cggccacctt taccgcgccg
     2521 tgctcgaagc gattggctac ggactacgca cgaatctcga ggtaatggcc gagatgaatg
     2581 cggcgccgaa acgcctggtg gcggtcggcg gcggcaccca gagcgacgtc tggacccaaa
     2641 tcgtctccga cgtcaccggg atggcacaga ttctgcccga aaagaccgtc ggcgcatcgt
     2701 acggcgacgc cctcctggcc ggtctcgcga cggggatcgt cgaccgggcg actggcatcg
     2761 acacctgggt tcgcacgaag cgggtggtgg cgccgaatgc cgcgaacacc gcaatccacg
     2821 accgcggcta cgacatctac cgcaatctct acctccatgc cagggacgat ttgcatgccc
     2881 tggcccaact cgatacggcg ttcgaccgtc cggcttgacg aaataccctc ccggggtatc
     2941 attggataat cttcggctga cggacagcgc gttccggcgg catcgcacat gcaccccaag
     3001 gagcatcgac atgacagcca tcaccgatcc cgccaacatc gagcacgtaa cgctttccac
     3061 cccggacatc ggctgcgatc actgcgtcgc gacgatccag gagaccctga gcgtcctcga
     3121 cggcatctcg agcgcgaggg catcgacgga gacgaagtcg gtggatgtcg tcttcgacgc
     3181 cgaagtgatt tcgctcggca agatcgaggc tgcgctcgag gaagtcggct accccgtcac
     3241 ccggtagcgc ctcgcttccc tgtttcgaat cggcaatccc agagagggcg gacccgggcg
     3301 atgatgcccg ggtccgcctt ttccttcata ccaccatgaa accgctggtg tagaatcgaa
     3361 ccagactgga aaacatcgcg agggcagcgg cgcctttggc ctggtatcga accggtcggg
     3421 ggcacgcagg ttgcgttaca ggatcgcgat cgaaagccga caccctcttc acatagaaga
     3481 ttccggccag cccagaaaag cgttcgaggc tcgttgggat cggcacgatc gaaactctgg
     3541 gcggatcgtt ttctgggagg ttcccccggg actgagggcg tcggagacat caggagtgac
     3601 tatcgtgccg agcacaagcg gaatgcacct cgtctcaagc gaagacaacg cggacgcgct
     3661 ccagcgcaag caggaggtcg tcgaacgcat actctcgacg caccccctgc tcgtcgcctc
     3721 caatcgcgga ccggtcacct tttctccccg tcaggacggg tcgttttccg cccgtcaggg
     3781 gagtggcggt ctcgtcaccg ccgtgcgcgc agttctcaag gaccatcagg cgatctggat
     3841 cgcggccgcg atgaatgaag gcgaccgctc ccgcgcgcag gtcgcggtcg acgacggcga
     3901 agacttcatc acggactccc ccgactccct cttcaagctt cgcttcgtca cgccctcccc
     3961 cgatgcctat catcagtact acaacgtcat cagcaatccg ctgctctggt tcctccagca
     4021 ctacctctgg gatacgccgc gctctccaga catcacccac gaaacgtggc gcgcctggcg
     4081 cgagggctac gtggcggtca accagcagtt tgcggacgag gtgctcgtcg cctgcgagcg
     4141 taccgaggcc gagccgatca tcatgctgca ggattaccag ctctaccttg tcggcaagat
     4201 gatccgcgaa aagcgtccgc aagcgatcat ccagcacttc atccacatcc cctggccaga
     4261 tcccgactac tggcgactcc tgccgatgga gatgcgccgc agcatctgcc agggtcttct
     4321 cgcctgcaac atcgttggtt tccagacgcc ggagaacgcc cgcagcttca tgtacacctg
     4381 cgaggcctat gtccccggtt gcgagatcga ctacatcgag cgaacggtga cgctcgatgg
     4441 ccacgtcacc gaggtccgtg cctatccgat ttcgatcgac gtcgaatcgg tccgccagac
     4501 ggcatacagc caggaagcgc gcgcctacga gcgctatctt ccctcccact ggaacgagta
     4561 cacgatcctc cgcatcgatc gcgccgagcc cagcaagaac atcgttcgcg gcttcgcggc
     4621 cttcgatcgc ttcct
//
LOCUS       DASTMD010000303         1523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1990821, whole genome shotgun sequence.
ACCESSION   DASTMD010000303 DASTMD010000000
VERSION     DASTMD010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990821"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFQ54_04465"
     CDS             <1..748
                     /locus_tag="VFQ54_04465"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU0164274.1"
                     /translation="FLYFFVRGLMNSKVDVAIANAHHIVNFERAIGIFREPRIQEWVT
                     KHEWLVTLFNSVYIYGHWPVLIGTLVWLLWRHRDYFPVFRSALLISGAMGLVVFVTFP
                     MAPPRFLPSLGFVDTVTLHTDAYRVLQPPAFTNQYASMPSLHVGWNLLMGVAIVMLSR
                     NRFAKVFGVLMPLTMYTATILTANHYFVDGIAGSIFAMVGLAIAWRISGPRAAGNAVP
                     EAPATPVAEETIPNMEFVPLDGPAPQHVAA"
     gene            complement(1011..>1523)
                     /locus_tag="VFQ54_04470"
     CDS             complement(1011..>1523)
                     /locus_tag="VFQ54_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0164275.1"
                     /translation="EVEALWGYIRDETIDFVCSDHVGIPIALKRAGDRDTFAAPLGVT
                     GAQTLLPVFWHEAVTKRRMSPSHVVRLLSTNPAQIFGFHPRKGTIRVGADADLALFDP
                     RSEWTIRNTDMLYQHPWTPFDGRTITGLPVRTIRRGETIYDAENHGNPDAVTSGTGRY
                     IPRGYGANGR"
BASE COUNT          242 a          498 c          494 g          289 t
ORIGIN      
        1 cttcctttac ttcttcgtgc gcggcctgat gaacagcaag gtcgacgtcg cgatcgcgaa
       61 cgcgcatcac atcgtgaact tcgagcgcgc gatcgggatc tttcgcgagc cccggatcca
      121 ggaatgggtg acaaaacacg agtggctggt cacgctcttc aactcggtct acatctacgg
      181 tcactggccg gtgttgatcg ggacgctcgt ctggctgctc tggaggcatc gtgactactt
      241 tccggtcttc cggagcgcgc tcctgatctc cggcgcgatg ggactcgtcg tcttcgtgac
      301 cttcccgatg gcgccgcccc gcttccttcc gtcgctcggc ttcgtcgata ccgtgacgtt
      361 gcacacggat gcctaccgcg tgcttcagcc gcccgccttc accaaccagt acgcctcgat
      421 gccgagcctc cacgtcgggt ggaatctgct gatgggtgtc gcgatcgtga tgctcagccg
      481 gaaccggttc gcgaaggtct tcggcgtgct gatgccgctg acgatgtaca cggcgacaat
      541 cctgaccgcg aaccactact tcgtcgacgg gatcgccggc tcgatcttcg cgatggtcgg
      601 tctcgccatc gcctggcgaa tcagcggacc ccgcgccgcc gggaatgcgg taccggaggc
      661 cccggccaca ccggtggcgg aggagacgat tccgaacatg gagttcgtcc cgctcgatgg
      721 tcccgctccc cagcacgtcg ccgcctgatt cttctcccga tacgctgatc ggctatccat
      781 caccctcgcg tccccacaga cgcgggggtg aattgtgtcc ggtcccgggc acgttcgctc
      841 acgcggggaa gagaagcggg acggcgtgcc gttcccgagt ccgcgttgcg gcggggcaga
      901 aaaccccgcc cgaccaaccc cggtaccacc agtcgtgtcg acggttgcaa acttcgcttt
      961 gggatcgggc accagctcat cacagggtgc cgtcatctcc ggacccgccg ctagcgaccg
     1021 tttgcgccgt agccgcgcgg aatgtagcga ccggttccgg aggtgacggc gtcgggattg
     1081 ccgtgattct cggcgtcgta gatcgtctcc ccacgccgga tcgtgcgaac ggggagtccc
     1141 gtgatcgtgc gcccgtcgaa cggggtccag ggatgttgat agagcatgtc ggtgttgcgg
     1201 atcgtccact cgctccgggg gtcgaagagg gcgagatcgg cgtcggcacc gacccggatc
     1261 gtccccttgc gcggatggaa gccaaagatc tgggcgggat tcgtcgagag caggcgaacg
     1321 acgtgggagg gactcatccg tcgcttcgtc actgcctcgt gccagaagac ggggagcagc
     1381 gtctgggcgc cggtgacgcc gagcggagcc gcgaaggtgt cgcggtcgcc ggctcgcttc
     1441 agcgcgatcg ggatgccgac gtgatccgag cagacgaaat cgatcgtctc gtcccggatg
     1501 tagccccaga gcgcctcgac ttc
//
LOCUS       DASTMD010000304         3122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_206449, whole genome shotgun sequence.
ACCESSION   DASTMD010000304 DASTMD010000000
VERSION     DASTMD010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206449"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1330)
                     /gene="dnaJ"
                     /locus_tag="VFQ54_04475"
     CDS             complement(194..1330)
                     /gene="dnaJ"
                     /locus_tag="VFQ54_04475"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU0164276.1"
                     /translation="MAEKRDYYEVLEVTRTANEQEIKRAYRSLARKYHPDINKEHDAE
                     DRFKEVNEAYEVLSNSERRAAYDRYGHAANGMGGGGGDPFGFGGGSPFQDIFESFFGG
                     GQAQSSGRRAAPPRGADIQVGVDLTFEEAVFGAEKDVEVDRLETCEACHGTRMKDGKQ
                     PPSCATCGGSGEVRRVQQTILGQFMTAGPCPTCHGEGVTITQPCDVCRGRGRTRSYRT
                     IAVTIPAGVDDNATLRLTGQGEDGPNGGPAGNLYVKVHVSPHKFFERHGKAIHSQVGI
                     NVAQAALGDEIEIDTLDGEVVFKIPTGTQSGQQFRLRGKGVPDLRGGDRGDQIVTIQV
                     VVPKKLSDEQKHLFEQLASSLGKEVQQQPNNRGFFDRVKDALGV"
     gene            complement(1682..2578)
                     /locus_tag="VFQ54_04480"
     CDS             complement(1682..2578)
                     /locus_tag="VFQ54_04480"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU0164277.1"
                     /translation="MTNAREDSFLGALLGLAIGDALGRPLVGLTAAEIHARHGEVAGY
                     VSDPGDEPETPPTGIITDETEVTLTIVESLTTNDGMLDPENINARLGFLIKGDSRRWM
                     PDGVISGIQEATETDGLVPVTGDGEVDLSVAIRGVAVGLLHSLGARDDETVRVDADLA
                     SRLSHGGSAQRLLTYEVALATRAAALGETGAIVAIADRLNGSGAGSSFASIVRGAAVV
                     AQFEDLVFAVVGEGGAADTRGALAGALAGARFGASGIPQDLIDHLDARIYLSLAAPWF
                     YRAAMRRAGMVIDLRNITQSEG"
     gene            complement(2662..>3122)
                     /locus_tag="VFQ54_04485"
     CDS             complement(2662..>3122)
                     /locus_tag="VFQ54_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164278.1"
                     /translation="QNPPGVLLEDVTSGNATFVIRAMGTRPKIEIASTSVESLGGDLY
                     RVAAVIQNTGFLPTFVSEKGKATGLIKPVRASIDGGDDVKIVSGKQEQDLGHLDGRAN
                     VFGSMSSTPQYGNLTRTRVEWIVSGAAGSEIELVVKTTKAGTVRTTVALG"
BASE COUNT          539 a         1040 c          992 g          551 t
ORIGIN      
        1 ccgaaacggc gacaagcgcc gtgaggacgg gatctggaga gcgtcgggga cacggaggtc
       61 ccatcgcggc ccgttcggtg taccggaggc gaagcaactc ctgcgcgagc acaggggagg
      121 aatgcaagaa gcccgacccc cctgaacgga gaatcgggct tcttgattga gcttatgtca
      181 tctcgcgcga aagctagacg ccgagggcgt ccttcacgcg gtcgaagaag ccgcgattgt
      241 tcggctgctg ctgcacttcc ttgccgaggc tggaagccag ttgctcgaag agatgcttct
      301 gctcatcact cagtttcttc ggcacgacga cctggatcgt cacgatctgg tcgccgcgat
      361 cgccgccacg aagatcgggg acacccttgc cacgcagcct gaactgctgt ccggattggg
      421 ttccggtcgg gatcttgaag acgacctcgc catccagcgt gtcgatctcg atctcgtcgc
      481 ccagcgccgc ttgcgcgacg ttgatgccga cctggctgtg aatcgccttg ccgtgccgct
      541 cgaagaactt gtgcggcgag acgtggacct tcacgtagag gttgccggcc ggaccaccgt
      601 tcggcccgtc ctcgccctgg ccggtcaggc gcagcgtcgc gttgtcgtcg acaccggccg
      661 gaatcgtcac ggcgatcgtg cggtaggagc gggtgcgacc gcgaccacgg cagacgtcgc
      721 atggctgcgt gatcgtcacg ccttcaccgt ggcacgtcgg gcagggcccg gcagtcatga
      781 actggccgag gatcgtctgc tgaacgcgac ggacctcgcc cgagccaccg caggtcgcgc
      841 atgaaggcgg ctgcttgcca tccttcatgc gtgtgccatg acatgcctcg catgtctcga
      901 ggcgatcaac ctcgacgtcc ttctcagcgc cgaagacggc ctcctcgaag gtgagatcga
      961 caccgacctg gatgtcggcg ccccggggtg gcgccgcgcg acgcccgctc gactgcgcct
     1021 gcccgccacc gaagaagctc tcgaagatgt cctggaaggg agatccccca ccgaagccga
     1081 acggatcccc gcctccgcca cccatgccgt tggcggcatg tccgtagcga tcgtaggcgg
     1141 cgcgccgctc ggaattcgag agcacctcgt aagcctcgtt cacttccttg aagcgatcct
     1201 cggcgtcatg ttccttgttg atgtcgggat gatacttgcg cgccaacgag cggtaagcgc
     1261 gcttgatctc ctgctcgttg gccgtccgag tgacctcgag cacttcgtag tagtcgcgtt
     1321 tttctgccat agtccatctt gatgggcggt cgatcggaag ggtttgagcc ggaaaatgcg
     1381 accgtcgttg aggcgaatgt cgtgcaagac tggaccccca gctgtgagaa gtataagcgc
     1441 tggcaagtca gcagccaacc actctggcac tcatgagact tgagtgccag atgtgagctt
     1501 catgtttcac tccggaggca tggcgagacg cgaaacggtt gccgaacgtg ctgccggatc
     1561 ggacgcggta ttccgcgctg cgcccggtcg ttcgtaaggc gggaccgccg ctgccggacc
     1621 gcagggccgg acggcccgtc ccgcctcacg cccaagggcc gtgctcccgg cggcgacggt
     1681 ttcagccctc gctctgcgtg atgttccgaa gatcgatgac cattccggca cgacgcatcg
     1741 ccgcacgata gaaccacggc gccgccagcg agagatagat ccgcgcgtcg agatgatcga
     1801 tgaggtcctg gggaatgccg ctcgcaccga accgtgcacc ggccagggcg cccgcgagcg
     1861 cgccgcgtgt atccgccgct ccgccctcgc ctacgacggc gaagacgaga tcctcgaact
     1921 gcgcgacaac cgccgcgccg cgcacgatcg aggcaaacga ggagcctgca ccagatccat
     1981 tgagacgatc ggcaatcgca acgatcgccc cggtctctcc cagcgccgcc gctcgcgtcg
     2041 ccagcgcgac ctcgtaggtc aacagtcgct gcgcactccc accgtgactg agccgcgacg
     2101 ccagatcggc gtccacacgc accgtttcat cgtcacgagc gccgagggag tggaggaggc
     2161 cgactgccac tccccggatc gcgaccgaga gatcgacctc gccgtcgccg gtcacgggca
     2221 cgaggccgtc agtttcggtc gcctcctgga tgccggagat cacgccgtcc ggcatccagc
     2281 gacgcgaatc gcccttgatc aggaatccga gccgggcgtt gatgttttcg ggatcgagca
     2341 tcccgtcgtt cgtcgtcagc gactcgacga tcgtcagcgt cacttccgtc tcgtcggtga
     2401 tgatcccggt cggcggcgtt tccggctcgt caccagggtc ggagacgtaa cccgctacct
     2461 ccccatgacg cgcgtgaatc tccgctgccg tcaagccgac gagtggacga cccagcgcat
     2521 cgccgatcgc gagcccgagc agggccccga gaaaactgtc ctcgcgcgcg ttggtcatgc
     2581 ggtgctccca tgcgttgtca gcgtcgacgt tgcgccggga tcgtgagaat catgaaagga
     2641 gacgccggtc gcgtcccaac tctagcccaa agccaccgtc gtgcggacgg tgccggcctt
     2701 ggtcgtcttc accaccagtt cgatctcgga gccggcggcc ccggagacga tccactcgac
     2761 ccgcgttcgg gtgagattgc cgtactgcgg tgtcgaggac atcgatccga agacattggc
     2821 ccggccatcg agatggccga ggtcctgctc ttgcttgccg gagacgatct tcacgtcgtc
     2881 accgccatcg atcgaggcgc gaaccggctt gatcagtccg gtcgccttgc ccttctcact
     2941 gacgaaggtg gggaggaacc cggtgttctg gatcacggcc gcgacgcggt agaggtcgcc
     3001 gccgagcgac tccactgatg tcgaggcgat ctcgatcttc ggtcgcgtac ccattgcccg
     3061 gatcacgaag gtggcgttgc cggaggtgac gtcctcgagc aacacgcccg gcgggttctg
     3121 cc
//
LOCUS       DASTMD010000305         1680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1990904, whole genome shotgun sequence.
ACCESSION   DASTMD010000305 DASTMD010000000
VERSION     DASTMD010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990904"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VFQ54_04490"
     CDS             complement(<1..643)
                     /locus_tag="VFQ54_04490"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEU0164279.1"
                     /translation="MKIELNFDDQIIHGEIALPDNQILWAESNDPPPTKEWEEIVLEA
                     LEAPIGTPRLRDHDLVGKKVVVIVDDWGRPTPASRVLPAILAEVEASGARREDITVMT
                     GSGVHEPMSKEDVIRKVGAQVFADYRCIAHDAVEHEMTFIGQSSRGTPVWVSKTVADA
                     DFRIAVGRIGPHPTHGYEGGAKMITPAVSYWMTVLRNHSTNFSPYAEYGSLLNN"
     gene            complement(655..>1680)
                     /locus_tag="VFQ54_04495"
     CDS             complement(655..>1680)
                     /locus_tag="VFQ54_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164280.1"
                     /translation="LLIDASRKRNMYTGAELSHTWIDKERVREEFPEIVQKNIYGNSI
                     DQRICFNHPDVRTYGIALYTDLVANYDIDMVQTCVRGFAESGSPWGRAADVTEIDRLL
                     SVVHGGCFCEHCAEKARSQYGLDWDAIVEKLRWLADGFDRFNARQAFDLALLRASTTS
                     VAALLLDTPELYAWLKFRTDSLTEFYQQIHDACHAVRDNIDVRLNHYAPYPELMGLDL
                     KGVAPFLDSVRSSDYQEQSGSMERVQGWKRGYLHGIRHAIGVEKYFTSAISPRPKATP
                     ELVRAGIRVSAECGANGLTLGHYDGAWLDCLKAVKDGLNDAGIVVLERDATAGPAHLA
                     HQHATAR"
BASE COUNT          275 a          531 c          557 g          317 t
ORIGIN      
        1 ggttgttgag caggctgccg tattccgcat atggcgagaa attcgtcgaa tgattgcgaa
       61 gcaccgtcat ccagtaggag acggccggcg tgatcatctt cgccccaccc tcgtagccgt
      121 gggtggggtg cgggccgatc ctgccgaccg cgatccggaa gtcggcgtcg gcgaccgtct
      181 tgctgaccca gaccggcgtg ccacgcgacg attgcccgat gaaggtcatt tcgtgctcga
      241 ccgcgtcatg cgcgatgcag cgatagtccg cgaagacctg cgccccgacc ttgcggatca
      301 catcctcctt gctcatcggc tcgtggacgc cgctgccggt catcaccgtg atgtcctcgc
      361 gtcgcgcgcc ggaggcttcg acctccgcga gaatcgccgg cagcacgcgc gacgcgggcg
      421 tcgggcggcc ccagtcatcg acgatgacga cgaccttctt cccgacaaga tcgtgatccc
      481 gcaggcgcgg ggtgccgatc ggcgcctcca gcgcctcgag cacgatctct tcccactcct
      541 tggtcggcgg cggatcgttg ctttccgccc agaggatctg attgtccggc agggcgatct
      601 cgccgtggat gatctggtcg tcgaagttca gctcgatctt catggtctcg cctcctacct
      661 cgctgtcgcg tgctgatggg ccagatgtgc tggtcccgcc gtcgcgtcgc gctcgagcac
      721 gacgatgccc gcgtcgttca ggccgtcctt caccgccttc aggcaatcga gccaggcgcc
      781 atcgtagtga cccagcgtca ggccgttcgc cccgcactcg gcggagaccc ggataccggc
      841 gcggacgagt tccggcgtcg ccttcgggcg cggcgagatc gccgaggtga agtacttctc
      901 gaccccgatc gcgtgccgga taccgtggag atacccgcgc ttccagccct ggacgcgttc
      961 catgcttccg ctctgctcct ggtagtcgga actgcggacg gaatcgagga agggggcgac
     1021 acccttcaga tcgagtccca tcaactccgg atagggcgcg tagtgattga gacggacatc
     1081 gatgttgtcg cgaaccgcgt gacaggcgtc gtgaatctgc tggtagaact cggtgaggga
     1141 gtcggtgcgg aacttcagcc aggcatagag ttccggagtg tcgaggagga gtgcggcgac
     1201 cgaggtcgtg ctcgcgcgga gcagggccag gtcgaacgcc tgccgcgcgt tgaagcgatc
     1261 gaagccgtcg gcgagccagc ggagcttctc cacgatcgcg tcccagtcga gaccgtattg
     1321 cgatcgcgcc ttctccgcac agtgctcgca gaaacagcca ccgtggacga cgctcaggag
     1381 gcgatcgatc tcggtcacat cggcggcgcg tccccatggc gagccggatt cggcaaagcc
     1441 gcgtacgcag gtctgcacca tgtcgatgtc gtagttggcg acgagatcgg tgtagagcgc
     1501 gatgccgtac gtgcggacat cgggatggtt gaagcagatg cgctggtcga tcgaattgcc
     1561 gtagatgttc ttctggacga tctccgggaa ctcctcgcgg acgcgctcct tgtcgatcca
     1621 ggtgtgggag agctcggcgc ccgtgtacat gttgcgcttg cgagaggcgt cgatcaggag
//
LOCUS       DASTMD010000306         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_295478, whole genome shotgun sequence.
ACCESSION   DASTMD010000306 DASTMD010000000
VERSION     DASTMD010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295478"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFQ54_04500"
     CDS             <1..407
                     /locus_tag="VFQ54_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164281.1"
                     /translation="ERIHELAGAGLDWGPCHGDATMDNFHVDDCQRIVWYDLDSGGPG
                     WRAGDLQGWAAIEPDAASLWDAFLRGYREVRPLDPREIEAAPYISAAFELWGTAVDLD
                     RRSIHQGEAAVATRLRSARDVLQRWADHFGFS"
     gene            complement(596..1012)
                     /locus_tag="VFQ54_04505"
     CDS             complement(596..1012)
                     /locus_tag="VFQ54_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0164282.1"
                     /translation="MSILDGAQPHILAINHSPEVLTLLEELLREEGYRVTTRSHLDRD
                     LRDFSTVSPDLIVIDYMWATDDDNWSLLQLLRMNPATRDIPIVLCTGAVREAQALESH
                     LFEMRIRVVYKPFDIGVLLGVIADALMMRDSIPGDG"
     gene            complement(1479..>2177)
                     /locus_tag="VFQ54_04510"
     CDS             complement(1479..>2177)
                     /locus_tag="VFQ54_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0164283.1"
                     /translation="LVKGAPDVRSAGTDWYYDFSADGIKRSIAESLKRLQLDRVDILY
                     IHDADDYVDQAVAEAWPVIEQLRSEGVVRAVCAGNTTVPTLLRFARETTMDFFLVAGR
                     YTMLADDAQDELLPLCIEKGIGIVIAQALHGGLIDGVADPQLYYQPTDDATKAKVARI
                     AAICHAYGVPTAAAAIQFPLAHPAVAGVLTGPETAEQLRGNLEWVTTDVPVAIWAELK
                     AEGLLRQDAVVPGA"
BASE COUNT          383 a          723 c          714 g          357 t
ORIGIN      
        1 cggaacggat ccacgagctg gcgggggcgg gtctcgactg gggtccctgt cacggcgatg
       61 cgacgatgga caacttccac gtcgacgact gtcagcgcat cgtctggtac gacctcgatt
      121 ccggtgggcc gggatggcgc gccggtgacc tgcagggatg ggcggcgatc gagcccgatg
      181 ccgcctccct ctgggacgcc tttctgcgcg gctaccggga ggttcgaccc ctcgatccgc
      241 gcgagatcga ggcggcgccg tatatctcgg cggcattcga attgtgggga accgcggtcg
      301 acctcgatcg tcgcagcatc caccagggcg aggcggccgt cgcgacgcgt cttcgctccg
      361 cgcgggacgt cctgcagcgc tgggccgacc acttcggctt ctcctgatcg agctccagaa
      421 ggtgggacat aggtcccgcc gctatggacg agccgcggtt ccctcccggg tcgggaactg
      481 gcgggcgcgc accggtagcg ccgggccgag gcaatgccgc ccacaacgca agcggggctc
      541 gatcggaaat ccgatcgagc cccgtcccgt tccgtgtgtg atcttcccgg ggaccctacc
      601 cgtcgccagg aatgctgtcg cgcatcatga gggcgtcggc aatcacgcca aggagcacgc
      661 cgatgtcgaa cggcttgtag acgacgcgga tccgcatctc gaagagatgg gattcgagcg
      721 cctgcgcctc ccgaaccgca cccgtgcaga ggacgatcgg gatgtccctg gtcgcgggat
      781 tcatccggag caactgcagg agcgaccaat tgtcatcatc cgtcgcccac atgtagtcga
      841 tgacgatgag gtcgggcgag accgtggaga agtcacgaag atcccggtcg agatgggaac
      901 gcgttgtcac gcgataccct tcctcccgaa gaagctcctc gagcagggtg aggacctcgg
      961 gggagtgatt gatcgccagg atatgcggct gcgctccgtc cagtatcgac accaggattc
     1021 cttctcgaag gggcaacacg tccgaacgtc acgcagaacc acgcctgcct cggaccttca
     1081 tcggcgacaa ggttaatgac acaggcgcaa gccggtcttc cgggtgagca atgagcgctt
     1141 cagtatacgt gcgatcgcgt gctggacaac ttcatgcgcg ttgccgtgga cgccccgagc
     1201 gggaggcggc tttgcggcta aggtttgtca tttcgcgcct gccgggaaat ccccgtgcga
     1261 agcacatccg gcgccgctgg actccagcac caacgatcgg gggaaggcgc tcccccgaaa
     1321 acggatcggc ggagccgacc cggaggcgac cacctgggtc gccgggacgg gaacggaacg
     1381 tgcatcggga cgcccggtcg tccacacatc cgcgtagccc cagcgtttgt gaaacgggga
     1441 tcaccacgtc ctctccgatc gtttcacgga cgatctccct acgctcccgg gacaaccgca
     1501 tcctgccgca acaagccttc cgccttgagc tccgcccaga tcgcgaccgg aacgtccgtc
     1561 gtcacccatt cgagattgcc gcgcagctgc tccgccgtct ccggaccggt cagcacaccc
     1621 gcgacggcgg gatgagcgag cgggaactgg atcgccgccg cggcggtcgg gacgccatac
     1681 gcgtggcaga tcgcggcgat ccgcgcgacc ttcgccttcg tcgcgtcgtc cgtgggctgg
     1741 tagtagagct gcggatcggc gacaccgtcg atcaggccgc cgtgcagcgc ctgcgcgatg
     1801 acgattccga tccccttctc gatgcagagc ggcagcaact cgtcctgcgc gtcatcggcg
     1861 agcatcgtgt agcgaccggc gacgaggaag aaatccatcg tcgtctcgcg agcgaagcgg
     1921 agcagcgtcg gcaccgtcgt gttcccggcg cagaccgcac gcacgacacc ttccgagcgc
     1981 aactgctcga tcaccggcca cgcctccgcg accgcctggt cgacgtagtc gtcggcatcg
     2041 tggatgtaga ggatgtcgac gcgatcgagc tggagtcgct tgaggctttc cgcgatcgag
     2101 cgcttgatac cgtccgcgct gaagtcgtag taccagtcag tgcccgccga gcggacatcc
     2161 ggcgcacctt tcacgag
//
LOCUS       DASTMD010000307         4792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2973710, whole genome shotgun sequence.
ACCESSION   DASTMD010000307 DASTMD010000000
VERSION     DASTMD010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2973710"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /locus_tag="VFQ54_04515"
     CDS             complement(<1..1760)
                     /locus_tag="VFQ54_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164284.1"
                     /translation="MSEKSGKMSSLKDQLASRPLSRRRFAGMGAGAFAAAMLSGAALP
                     AGLGRVAMAQDGGKEFHSAYPYTAPPTGHFNSFASDGNGILQGSTTVYGDLIIEPLGM
                     YYWATGTWEPLLATDWAFIKSTTAATPGAIPVASPVPASIFDGTSALAGKAAANAPAD
                     ADTFIVRLRAGTKWEDGTPITSKDVVDTLWCLRIMSNVVWTLIDDAVVIDDSTFSVHM
                     STPATVVERYIIRQSNPRPSSIFGDWAQKARDLFASGKTIDDDEGKQLLDQFNKFRPD
                     KVVASGPYTIDISSITSAQMTLPKNPNAWNADKALFDRIVNFNGETDTITPVVLAKEV
                     DYATHGFAPASEKQMEASGIRILRPPTYSGPAVYMNFDKLASTFGDVKVRQAVATAIN
                     RAQNGTVALADSGIGVKYMTGFSDNLVAQWLDDAAIKGLDDYANDADKAASILTDAGW
                     TKDGDNWKDKDGKETKFEVTFPAEYADWSASGLDFADQLSNWGIQVTPRAVTYTQQPI
                     DMDKGNFELGIQGWGNSNNPHPYFSFVTPFLTRNFPIAKNNGGKGIDFPLDQTLADGT
                     KVNIQDLINASGQGLDAEKQ"
     gene            complement(2056..3099)
                     /locus_tag="VFQ54_04520"
     CDS             complement(2056..3099)
                     /locus_tag="VFQ54_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0164285.1"
                     /translation="MSEPLMVLDNLVRTFDTRRGPITAVDHVSLSLNAGDILCLVGES
                     GSGKTTVARMAAGLLDPSSGSVRFDGRDLKQMSKAEFKEYRKAVQIIHQDPYASLNPV
                     HTIGETLRAPLLHHNLVKGGKEATKRVEELLELVDLRPAENFINKFPHQLSGGQRQRV
                     SVARAMALNPRIIIADESVSMVDVSIRVSLLNTMLKLRNELGVTFLFITHDLAVVKYF
                     AWEGEVGVMYVGKLIEHGPTPQIIGRSMHPYTRALAAAIPEADPDLTRSKEKLQLRSL
                     DIPNLANLPTGCVFNPRCPYWEPGLCDVTMPPLVNIGDREVACHLVSRDIEQGGNGLN
                     LERVRVVAGQAAD"
     gene            complement(3166..4290)
                     /locus_tag="VFQ54_04525"
     CDS             complement(3166..4290)
                     /locus_tag="VFQ54_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0164286.1"
                     /translation="MAVDEKASRYTPATAEAAARNALRGTAQSTSPVILSIRDMFVEY
                     KTSQGWMEAVRGVNLDIRAGEAVALIGESGSGKSTLGFAILRMLVRSARISKGSAMYR
                     DRDGKEFSLLDIKDRDFRRIRWSEIAMVFQAAQNSLNPVMRVRDTFYETARAHGMTDK
                     RAVREKTLELLTMVQLDADRVYNAFPFELSGGMRQRVSIALSLLLDPQLIILDEPTTA
                     LDILTQRAIIDVVKSLRDKLEFTMIFISHDLSLAAELADRVATMYAGEVVEYGPVRDV
                     FYTPKHPYSVGLLNAVPPIIGEEFTPLTSIPGSTPNLLRMPSGCPFHPRCPYATEICS
                     QQDPPLIDLGGTHFVACHHHDLVTKDPAIWERMENIEVAG"
     gene            complement(4357..>4792)
                     /locus_tag="VFQ54_04530"
     CDS             complement(4357..>4792)
                     /locus_tag="VFQ54_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0164287.1"
                     /translation="PLLLAVVLAATAWPALLRAVRAQVLSLKEREYVEAARMLDLGTP
                     RILFREVIPNMMSYIVINFVFAMTSAIYGQVVLFFLGLVPLAGNNWGLMINEAYTKGA
                     LFYKDSVLYILAPIFMVVILQLSLVMISRSLEDVFNPRLREE"
BASE COUNT          852 a         1445 c         1572 g          923 t
ORIGIN      
        1 ttctgcttct cggcgtcgag cccctgaccg gaggcattga tcaggtcctg gatgttgacc
       61 ttggtgccgt cggcaagtgt ctggtcgagc gggaagtcga tacccttgcc gccgttgttc
      121 ttggcgatcg ggaagttgcg ggtcaggaac ggggtcacga acgagaagta cgggtgcggg
      181 ttgttggaat taccccaacc ctggataccc aactcgaagt tgcccttgtc catgtcgatc
      241 ggctgctggg tgtaggtgac cgcgcgcggc gtcacctgga tgccccagtt ggagagctga
      301 tcggcgaaat cgagaccgga ggcggaccag tcggcgtact cggccgggaa ggtgacttcg
      361 aacttggtct ccttgccgtc cttgtccttc cagttgtcgc cgtccttggt ccagccggca
      421 tcggtcagga tcgacgccgc cttgtccgca tcgttggcat agtcgtcgag gcccttgatg
      481 gccgcgtcgt cgagccactg ggcgacgagg ttgtcggaga agccggtcat gtacttcacg
      541 ccgatgccgg aatcggcaag ggcgaccgtg ccgttctgcg cgcggttgat cgcggtggca
      601 acagcctggc gcaccttcac gtcaccgaac gtggaggcga gcttgtcgaa gttcatgtag
      661 acggccggac cggagtatgt gggcggacgc aggatccgga tgccagaggc ttccatctgc
      721 ttctcggaag ccggcgcgaa accgtgggtc gcgtagtcga cttccttggc aagcacgacc
      781 ggggtgatcg tgtcggtctc accgttgaag ttgacgatgc ggtcgaagag cgccttgtcg
      841 gcgttccagg cattcgggtt cttcggcagc gtcatctggg ccgaggtgat cgacgagatg
      901 tcgatcgtgt acggaccgct ggcgacgacc ttgtccgggc ggaacttgtt gaactgatcg
      961 aggagctgct tgccctcgtc atcgtcgatc gtcttgccgg aggcgaagag atcgcgcgcc
     1021 ttctgggccc agtcaccgaa gatcgaggac gggcgtgggt tggactggcg gatgatgtac
     1081 cgctcgacga ccgtggccgg cgtgctcatg tgcacgctga aggtggagtc atcgatgacg
     1141 accgcatcgt cgatcagcgt ccagacgacg ttggacatga tgcggaggca ccacagggtg
     1201 tcgacgacgt ccttcgaggt gatcggcgtg ccgtcttccc acttcgtgcc ggcgcggagc
     1261 cgcacgatga aggtatcggc atcggcggga gcattcgccg cggccttgcc ggcgagagcg
     1321 ctcgtcccgt cgaagatgct cgcgggaacc ggcgaagcga cgggaatcgc acccggggtc
     1381 gccgcggtcg tcgacttgat gaacgcccag tccgtcgcga gcagtggctc ccaggtcccg
     1441 gtggcccagt agtacatgcc gagcggctcg atgatcaggt caccatagac cgtggtggaa
     1501 ccctggagaa tgccattgcc atccgacgcg aacgagttga agtgacctgt cggcggcgcc
     1561 gtatacgggt acgcgctgtg gaattccttc ccgccgtcct gagccatggc gacgcgaccg
     1621 agacccgccg gaagcgcggc gccggaaagc atcgccgccg cgaaggcgcc tgctcccata
     1681 cccgcaaagc gtcgtcgaga cagcgggcga ctggccagct ggtccttcag acttgacatc
     1741 ttcccagatt tctccgacat gatgctctcc tgttcattga ccggtggaac gactccctcg
     1801 gccagcaacg tttcaggacc ccgatgccca tcccgcgcgc cctcgatcga ttcgagcccg
     1861 ctcgcctggc gacacctcta tcgtgcggcg cacagatcgt cttgcgcaac acgcgcgatt
     1921 tccggtcgtg agaacttcct ggatgatact ccaatccgcc cacacgtacg atccgaaagt
     1981 cctactgcca tcaccctggt ttcccgccgc ttccggcgtt caccatggga ccgcaggatt
     2041 cggacagacc gccaactagt cggcggcctg tccggcgacg actcgcaccc gttcgagatt
     2101 gagtccattg ccaccttgtt cgatgtcccg cgaaaccagg tgacatgcga cctcacgatc
     2161 cccgatgttg acaaggggcg gcatcgtgac gtcgcagaga cctggttccc agtagggaca
     2221 tcgcggattg aagacgcatc ccgtgggcag attggcgagg ttggggatgt cgagactgcg
     2281 cagttgaagc ttttccttcg atcgtgtgag atcgggatcg gcttcgggga tcgccgctgc
     2341 cagcgcccgt gtgtacgggt gcatcgagcg gccgatgatc tggggcgtgg gtccgtgctc
     2401 gatcagcttg ccgacgtaca tgacgccgac ctcaccttcc caggcgaaat acttcacgac
     2461 cgcgaggtcg tgggtgatga agaggaaggt gacgccgagc tcgttgcgaa gcttgagcat
     2521 cgtgttgagc agactgacgc gaatcgagac gtccaccatc gagaccgatt cgtcggcgat
     2581 gatgatccgc ggattcaggg ccatcgcccg cgccacagaa acgcgctggc gttgaccgcc
     2641 ggagagctgg tgggggaact tgttgatgaa gttttcagcc gggcgcaggt cgacgagttc
     2701 gaggagttcc tcgacccgct tcgtcgcctc cttgccgcct ttcaccaggt tgtggtggag
     2761 cagcggcgcc cgcagcgtct cgccgatcgt gtgcacggga ttcagggacg catacggatc
     2821 ctggtggatg atctggaccg ccttgcgata ctccttgaat tccgccttcg acatctgctt
     2881 gagatcgcgt ccatcgaagc ggacgctccc ggatgatgga tcgagcagac ccgccgccat
     2941 ccgggcgacg gtcgtcttgc cgctcccgct ctctccgacc aggcagagga tatcgcccgc
     3001 gttgagcgag agggagacgt gatcgacggc agtgatcggc cctcgccgtg tgtcgaacgt
     3061 tcgtaccaga ttgtcgagga ccatcaacgg ctcgctcatc ggcctacttt ccttggtgca
     3121 tcagggattc gttcgcacga acccggcgcg atcggcgccg aaacgctagc cggcaacttc
     3181 gatgttctcc atccgctccc agatcgccgg atccttggtc acgagatcgt gatggtggca
     3241 ggcgacgaaa tgcgttccgc cgaggtcgat cagcggcgga tcctgctgcg agcagatctc
     3301 ggtcgcatac gggcaccgcg gatggaacgg gcagccggag ggcatgcgca gcaggttcgg
     3361 ggtcgacccc gggatcgagg tcagcggcgt gaattcctcg ccgatgatcg gcggcacggc
     3421 gttcaggagg ccgacggaat agggatgctt cggcgtgtag aagacgtcgc gcaccgggcc
     3481 gtattcgacg acctcaccgg catacatcgt ggcgacgcga tcggcgagtt cggcggcgag
     3541 cgagaggtcg tggctgatga agatcatcgt gaactcgagc ttgtcgcgca ggctcttcac
     3601 gacgtcgatg atcgcgcgct gagtgaggat gtcgagtgcc gtcgtcggct cgtcgaggat
     3661 gatcagctgc ggatcgagga ggaggctcag cgcgatcgat acgcgctgtc gcatgccacc
     3721 ggagagttcg aaggggaagg cgttgtagac gcgatccgca tccagctgca ccatcgtcag
     3781 cagctcgagc gtcttctccc ggaccgcccg cttgtcggtc atgccatggg cgcgcgccgt
     3841 ttcgtagaag gtgtcgcgaa cgcgcatcac gggattcagc gaattctgcg cggcctggaa
     3901 gaccatcgcg atctcgctcc agcgaatccg gcgaaagtcg cgatccttga tgtcgaggag
     3961 gctgaactcc ttgccatccc gatcgcgata catcgcggat cccttgctga tgcgggcgct
     4021 ccgcaccagc atccggagga ttgcgaagcc cagcgtcgac ttgccggagc cgctctcgcc
     4081 gatcagggcg accgcttcgc cggcccggat gtcgagattg accccgcgca ccgcttccat
     4141 ccagccctgg ctcgtcttgt actcgacgaa catgtcgcga atggagagga tcaccggtga
     4201 ggtggattgc gccgtgccgc gaagggcatt gcgagcggcg gcctccgccg tcgccggtgt
     4261 atatcgggat gctttttcgt caactgccat gaactagttc acctttgcga cgtccggatc
     4321 gcggtatgct tgcgagtttt tgtggcgatg ccccgcctat tcctcgcgca gtcgcgggtt
     4381 gaagacatct tccagcgacc gggaaatcat gaccaacgag agttggagga tcacgaccat
     4441 gaagatcggc gccaggatgt agaggacgct gtccttgtag aacagcgcgc ccttcgtgta
     4501 agcctcgttg atcatcagtc cccagttgtt cccggccagc ggcacgagac cgaggaagaa
     4561 gagcacgacc tgaccataga tggcgctcgt catcgcgaag acgaagttga tgacgatgta
     4621 gctcatcatg ttcgggatga cttcgcggaa gagaatccgc ggcgtcccga ggtcgagcat
     4681 ccgcgccgcc tcgacgtatt cacgttcctt cagcgagagc acctgggcac ggacggcgcg
     4741 aagcagcgcg ggccaggccg tcgccgcgag caccaccgcg agaagcagcg gg
//
LOCUS       DASTMD010000308         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_116375, whole genome shotgun sequence.
ACCESSION   DASTMD010000308 DASTMD010000000
VERSION     DASTMD010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116375"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VFQ54_04535"
     CDS             complement(<1..969)
                     /locus_tag="VFQ54_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164288.1"
                     /translation="MSAERAMPASSWWKRTRQDVALLLRVHRLLLREDWTRGRILIAI
                     LCLQAAIPIAQLWLTRAIVNALVGGNSDAAIGFGVATVVAVLVFQIVTPIQMVLASQI
                     QDGGVAEIEREMIRAGSKLRDLTAIGSAGYLNRMNAIRDGAMEFGMTISRAGRFYAGA
                     VSLIGILISLIALQPLMPILLIATGYLAHRFSRRQADLEYASLWAVARSGAEMRYAQH
                     VATRAESAAEARMFGLGPWLRARYLRFGGETLAELGARRRSGALWAIFGTALHVVALA
                     GSFWYVARQAESGALSVGDLALFINALIQGQFLTQDLFVSVQTLRRI"
     gene            complement(966..>2276)
                     /locus_tag="VFQ54_04540"
     CDS             complement(966..>2276)
                     /locus_tag="VFQ54_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0164289.1"
                     /translation="LAPVVLLLAAIPRIANAYALADRTSSHIATQSPLARKLGYLRDA
                     PVAPHLARDFRLFELGDRTLERYDIVWREVTGSLNALRRQLLRTQVIGVAISEVVAGG
                     LVLLVIWKATRGDLTIGEVTLYTGAILMLNERVRQLGMMFGYVPMILAFLPSLQRILD
                     ASTDLPVEADPAPLTVPLREGFVFDRVTFAYPHATRPILKDVSFALAPGERLALVGHN
                     GAGKTTLVKLMLRLYDPDEGRITLNGIDLREYDPKALRHACSVIFQDFGRYDFTVRDN
                     IALGDIDRRHSDAELMEAAAKAGAEPVIATLDEGLDTLLGRRFGGRDLSGGEWQKVAL
                     ARAFVRDAPILVLDEPTAALDVRAEYEVYQRFADLTRGRATLLISHRFSTVRMADRIL
                     VLDENRLVEDGSHAELMARRGVYHRLYSAQAEHYVDMADGSVAP"
BASE COUNT          436 a          737 c          756 g          347 t
ORIGIN      
        1 aatccgccgc agcgtttgca ccgacacgaa gagatcctga gtcagaaatt gtccctggat
       61 cagggcgttg atgaagagcg cgagatcgcc gaccgagagc gcgccgctct ccgcctgccg
      121 ggcgacgtac cagaacgatc ccgcgagggc gacgacgtgc agtgctgttc caaagatcgc
      181 ccacagcgca ccggagcgcc gccttgcgcc gagctcggcg agcgtctccc cgccgaaacg
      241 gagatagcgc gcccgcagcc acggtcccag cccgaacatt cgcgcctctg cggcggactc
      301 cgcccgcgtc gcgacatgct gggcatagcg catctcggcc ccggatcgcg cgaccgccca
      361 gagcgacgcg tactcgagat cggcctggcg gcgggagaag cgatgcgcca ggtaaccggt
      421 cgcgatgagc aggatcggca tcaacggctg cagcgcaatg agggagatca ggattccgat
      481 cagggagacg gcgccggcgt agaacctgcc cgcccgcgag atcgtcatcc cgaattccat
      541 cgcgccgtcg cgaatcgcgt tcatccggtt cagatagccg gcgctgccga tcgccgtcag
      601 atcgcgcagc ttcgagccgg cacggatcat ctcgcgctcg atctcggcga caccgccatc
      661 ctggatctgg ctggcaagga ccatctgaat cggcgtcacg atctggaaga cgaggacggc
      721 gacgaccgtc gctactccga atccgatcgc ggcatcactg ttgccgccga cgagcgcgtt
      781 gacgatcgcc cgcgtcagcc agagctgggc gatcgggatc gccgcctgca gacagaggat
      841 cgcgatcagg atccggccgc gcgtccagtc ctcccgcagc agcaggcgat gaacgcgcag
      901 gagcagcgcg acgtcctgcc gtgtccgctt ccaccacgac gatgcaggca tcgctcgttc
      961 cgcgctcatg gggccacgct cccatcggcc atatccacat aatgctccgc ctgtgccgaa
     1021 tagagcctgt gatagacgcc gcgtctcgcc atcagctcgg cgtgggatcc gtcctcaacg
     1081 aggcgattct catcgaggac gaggatgcga tcggccatcc gcacggtcga aaagcggtgg
     1141 gagatcagga gcgtcgcgcg gccgcgggtg agatcggcga agcgttgata gacctcgtat
     1201 tcggcgcgaa catcgagcgc ggccgttggt tcgtcgagga cgaggatcgg cgcgtcgcgg
     1261 acgaaggcgc gagcgagcgc gaccttctgc cactcgccgc cggagaggtc gcgaccgccg
     1321 aaacggcgtc cgagcagtgt atccagcccc tcgtcgagtg ttgcgatcac cggctccgcg
     1381 ccagccttgg cagcagcttc catcaactcg gcatcggaat ggcggcgatc gatatctccg
     1441 agcgcgatgt tgtcgcgaac cgtgaaatcg tagcgaccga aatcctggaa gatcacgctg
     1501 caggcgtgac gcaatgcctt ggggtcatat tcgcggaggt cgatcccgtt gagtgtgatc
     1561 cgtccctcgt cggggtcgta gagacgcagc atcagcttga ccagggtcgt cttcccggcg
     1621 ccgttgtgac cgacgagtgc caatcgctcc cccggcgcga gcgcgaacga gacgtccttc
     1681 aggatcggac gtgtggcgtg cgggtacgcg aaggtgacgc gatcgaagac gaagccctcc
     1741 cggagcggaa cggtcaacgg ggcaggatcg gcctccaccg ggagatccgt cgaggcatcg
     1801 agaatccgct gcagcgaggg aaggaaggcg agaatcatcg ggacgtagcc aaacatcatc
     1861 ccgagttgac ggacgcgctc gttcagcatc aggatcgcgc cggtgtagag cgtgacctcg
     1921 ccgatcgtga gatcgccgcg ggtcgccttc cagatcacga gcaggacgag cccaccggca
     1981 acgacctcgc tgatcgcgac accaatcacc tgcgttcgca ggagttgccg gcgcaaggcg
     2041 ttgagcgatc ccgtcacctc acgccagacg atgtcatagc gctcgagcgt gcggtcacca
     2101 agttcgaaga ggcgaaaatc acgcgccagg tgcggcgcaa ccggcgcgtc tcgcaggtat
     2161 ccgagtttcc gggcgagggg gctttgcgtg gcgatatggg aggaggtcct gtcggcgagc
     2221 gcgtaggcgt tggcgatcct gggaatcgcc gccagcagca gaacgaccgg cgccaa
//
LOCUS       DASTMD010000309         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_386333, whole genome shotgun sequence.
ACCESSION   DASTMD010000309 DASTMD010000000
VERSION     DASTMD010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386333"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFQ54_04545"
     CDS             <1..888
                     /locus_tag="VFQ54_04545"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEU0164290.1"
                     /translation="FQDKQFTVIEGDTQDVGHGTNLSIRLDKFTDTYSEMGVASQYTS
                     DITILDDGKDVKSGQVTPNHPVSYNNATIYQASLAYAKQFKITDTAGNVIFDDASTIG
                     IFNQKGNPDAPAGFVDLPAINSQLIFVGPDAAPFNQPQLDTLHLTSNQLWVQLSTKDS
                     SGNYVSTPGQKITLNQPVKIGGYTIAWNGTRNVSMLQVAYNPGVPIFIIAAVLLVGGL
                     VVIFYFPHRRVRGIATTTPAGATLQLAPLAKRDWSGKHDFVRLIEAAQARLGEPQELK
                     LPKSGSEWESYTRSSSASS"
     gene            1016..1924
                     /gene="ccsB"
                     /locus_tag="VFQ54_04550"
     CDS             1016..1924
                     /gene="ccsB"
                     /locus_tag="VFQ54_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEU0164291.1"
                     /translation="MDGLVRFSFYCFAASSMSLWVAAAAYIVYAVGRVRVSQRTMMTS
                     AGTSVTSTGAEFGPGEIGFGRIGTMMAFFGAFFAGISILLRAIAANHVVLSNMYEYSL
                     VFVFFCSTSYLIIERVYGVRQLGAIVITIAALMTAYIWYLPAEMREINPVVPALQAGM
                     IMPFHVGSANLAYSVLTVSFGAAVFFLIANRWAVRWLPSAEMLDDIAFRAVTVGFPFL
                     SLMLILGSVWAHSAWGSYWSWDPKETSALFSWLVYAIYLHTRTLCGWRGQRSAYILIF
                     GFAALIFTYYGNYFFGGLHAYGGVQG"
     gene            2036..>2622
                     /locus_tag="VFQ54_04555"
     CDS             2036..>2622
                     /locus_tag="VFQ54_04555"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0164292.1"
                     /translation="MADAHVPEPTAQPSTPTRGVKPNPGKPSSIFDQPWPRLALVLVI
                     VLGIVGVTFAVGQKEGWTSIGDGGENAQLLPKVGQKAPELITATSDGSPVFLSTLIGH
                     PVWLNFWGSWCEPCQAEMPEIEAAYKVLQPQGVVMLAISQKEDVQTSVQYAEAAGATY
                     PIMMDPAEALSGIDPNDPSMPPALRSMIQQTATWQV"
BASE COUNT          492 a          907 c          740 g          483 t
ORIGIN      
        1 ttccaggaca agcagttcac cgtgatcgag ggtgacaccc aggatgtcgg gcacggcacc
       61 aacctctcga tccggctcga caagttcacc gatacctata gcgagatggg tgtggcgagc
      121 cagtacacga gcgacatcac gattctcgac gacggcaagg acgtgaagtc cggccaggtc
      181 acgccaaacc atcccgtcag ctacaacaac gccacgatct accaggcatc gctcgcctac
      241 gcgaagcagt tcaagatcac ggatacggcc ggcaatgtta tcttcgacga tgcttccacg
      301 attggcatct tcaatcagaa gggcaacccc gacgcccccg cagggtttgt cgatctcccg
      361 gcaatcaata gccagctcat cttcgttggt cccgacgctg cccccttcaa ccagccgcag
      421 ctcgacacgc tgcacctgac ttcgaaccaa ctctgggtcc agctcagcac caaggacagc
      481 agcggcaatt acgtcagcac gccgggacag aaaatcacac tcaaccaacc ggtcaagatc
      541 ggcggctaca ccatcgcctg gaacggcacg cggaatgtct ccatgctcca ggtggcgtac
      601 aaccccggcg ttccgatctt catcatcgcg gccgtgctac tcgtcggtgg tctcgtcgtg
      661 atcttctact tcccgcaccg ccgggtccgg gggatcgcca cgacgacacc ggccggcgcc
      721 accctccagc tcgcaccgct cgccaaacgc gactggagcg ggaagcatga ctttgttcgt
      781 ctcatcgagg ccgctcaggc caggctcggc gagcctcagg agctgaagct gccgaagtcc
      841 ggaagcgaat gggaatcgta tacccgatca tcgtccgctt cgtcctgaag gtgaactgca
      901 ccacgacacg acgacgcaca catcaccaaa tcgcatacat aatcatgagg acatacaata
      961 gcggcgacaa cgctcgccgc gacggcgtcc gagacggggg aggacaggaa cacagatgga
     1021 tggtctcgtc aggttttcat tctattgctt cgccgccagt tccatgtccc tctgggtcgc
     1081 tgcagctgcc tacatcgtgt atgccgtggg tcgcgttcgc gtctcccagc ggacgatgat
     1141 gaccagcgcg ggcacctcgg tcacgagcac cggggccgag ttcggcccgg gcgagatcgg
     1201 cttcggccgc atcgggacga tgatggcgtt cttcggcgcc ttcttcgccg ggatctcgat
     1261 cctcctgcgc gcgatcgccg ccaatcatgt cgtcctctcg aacatgtatg aatactcgct
     1321 ggtcttcgtc ttcttctgct cgaccagcta cctgatcatc gagcgcgtct atggtgtccg
     1381 ccagctcggc gcgatcgtga tcacgatcgc cgcgctgatg accgcctaca tctggtacct
     1441 gcccgcggag atgcgcgaga tcaatcccgt cgtcccggca ctccaggccg gcatgatcat
     1501 gccgttccac gtcggcagcg ccaatctcgc ctactccgtg ctcacggttt ccttcggcgc
     1561 ggcggtcttc ttcctgatcg cgaaccgctg ggccgtgcgg tggctcccga gcgccgagat
     1621 gctcgacgac atcgccttcc gcgcggtgac ggtcggcttc ccgttccttt ccctgatgct
     1681 gatcctcggc tccgtctggg cacacagcgc ctggggttcc tactggtcct gggacccgaa
     1741 ggagacatcc gcgctcttct cctggctcgt ctacgcgatc tacctgcaca cgcggacact
     1801 gtgcggctgg cgcgggcagc gcagcgccta catcctgatc ttcggcttcg cggcgttgat
     1861 cttcacctac tacgggaact acttcttcgg cggtctccac gcctacggcg gtgtccaggg
     1921 ataagcccgg gacgcagacc caaccaagac gaccactgac gtcggtcgtt ccggacctcg
     1981 cgcaacgttg ccgaccggaa cgaccgacat tcgctttcac caggaccaca tcttcatggc
     2041 ggacgcccac gttcccgaac ccaccgctca gccatcgacg ccgacccgcg gcgtgaagcc
     2101 aaacccgggc aagccgtcga gcatcttcga tcagccgtgg ccacgcctcg cactcgtcct
     2161 cgtgatcgtg ctcggcatcg tcggtgtcac cttcgctgtc ggacagaagg aaggctggac
     2221 gtcgatcggc gacggcggcg agaacgcgca actcctgccg aaggttggtc agaaggcgcc
     2281 cgaactgatc accgcgacat ccgacggcag ccccgttttc ctctcgacgc tcatcggcca
     2341 ccccgtctgg ctgaacttct ggggctcctg gtgcgagccc tgccaggccg agatgccgga
     2401 gatcgaggcg gcgtacaagg ttctccagcc gcagggtgtc gtcatgctcg cgatcagcca
     2461 gaaggaggac gtgcagacgt ccgtccagta tgcggaggcg gccggcgcga cctatccgat
     2521 catgatggac cccgccgagg cgctctccgg catcgacccc aacgacccgt ccatgccacc
     2581 agcgctcaga tcgatgattc agcagaccgc tacctggcaa gt
//
LOCUS       DASTMD010000310         4707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2348984, whole genome shotgun sequence.
ACCESSION   DASTMD010000310 DASTMD010000000
VERSION     DASTMD010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2348984"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /locus_tag="VFQ54_04560"
     CDS             <1..1571
                     /locus_tag="VFQ54_04560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0164293.1"
                     /translation="SPDGKTIAYTLNVDPENPEETPLDPKAPAKVRVVRNADYKFDGA
                     GYRGQTKTQVFTLDVASGTKKQLTSAQVDHMNLCWSPDGKNLAVTLWFDGPFSTAIAL
                     IDPETGDTTRLTEPDTMTGSLSWSPDSGKLFYDYEQFGYRILDVASKTVREVSADIGF
                     TPDSQATNPAVWRDEKTVLVHGYTGTGSGLWTIDIETGTPTQIVSWDAIHGGLTPIPG
                     SDTLLQSVGDLGGTAGVATIDPATGAKTLLFDEGTPFASHTPPAQWELISIERTGYKI
                     EAILLKPSNFDARRTYPIILDVHGGPQGAHMQRIEPNSQVMASNGFLVLMPNPRGSTN
                     YGREFSEAVYDDWGNEDWKDLQAILDKALELPYVDKDRTGIYGYSYGGYMTSWAIGHT
                     DRFKAAVCGAPPFDLESMYGTSDVSYVLGHKQWGGTPWEGREEYKTISPSEYIHNATT
                     PTLIVHGEADERCPIGQGEQMFISLKKLGVETQFVRYPGGSHGFAWRGEPAHRIDFYT
                     RILAWFKKYLGDPV"
     gene            complement(2109..2768)
                     /locus_tag="VFQ54_04565"
     CDS             complement(2109..2768)
                     /locus_tag="VFQ54_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEU0164294.1"
                     /translation="MRIQIIEDTVCPWCRIGKHNLHQALDEWQKTNDEPVEFEYLPFL
                     LDPVEPGSKENFRERFTTRKGIPADSIATMFDRVVGVGKQAGITFNFDKIEVAVDTVP
                     SHELILLTPQEKKADVIEAVMKAYFEDGKDIGEDDVLIQCARDAGLTDEQLAVIEPLL
                     RSHAMRDRVLAIIGSVQQAGITGVPFFIIDGKLAVSGAQPPTHFLQAFAQASTMEAVA
                     D"
     gene            complement(2847..4421)
                     /locus_tag="VFQ54_04570"
     CDS             complement(2847..4421)
                     /locus_tag="VFQ54_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEU0164295.1"
                     /translation="MTHHDIQVIIVLLLAVVSLVTLATRINVPFPMTLLIGGMILALI
                     PGLPRIELEPDLVFLLFLPPILFYAAYFTSWRDFKANLRPIGLLALGCVTLTTFVVAW
                     VARMLMPDMPWSVAITLGAIVSPPDAIAATAIFARLGAPRRVVTILEGESLVNDASAL
                     ILYRFGVAAVVGGTFSLLDASMTFVVLCVGGVALGLACAWLMDKIVPLITDSSVAITF
                     TLLAAIGIYVLAEAVHVSGVLATVAGGIYFGRHSSRALSSEDRVGGKIVWDTVVLAIN
                     GLVFILIGLQLPHLIDAIEGTSMTTLILQALAISAVVIVARLVWLFPSAYLPRLLIPG
                     ILARDPYPPIGNVILIGWSGLRGVVSLASALALPLTLDNGQPFPYRDRVIFLTFGVIV
                     VTLLGQGSLIPFMMRRLRVTDDGSVEKEIKHARALAAKAAIARLDEVQREEWVPQDHQ
                     AVLRYRFMHELQHFREEDHDHAREHHEQTVRLHNEVLQAARQAVVNARDRGEIGDEAR
                     VRIEQDFDLEQLAKHV"
BASE COUNT          914 a         1546 c         1485 g          762 t
ORIGIN      
        1 ggtcgcccga cggcaagacg atcgcctaca cgctcaatgt cgatccggag aatccggagg
       61 agacgccact cgatccgaag gcgcccgcca aggttcgtgt cgttcgcaat gccgattaca
      121 agttcgatgg cgccggctac cgtggccaga cgaagacgca ggtcttcacg ctggatgtcg
      181 cgtccggcac gaagaagcag ctgacctcgg cgcaagtcga tcacatgaac ctctgctggt
      241 cgcccgacgg caagaacctc gcggtgacac tctggttcga cggtccgttc tcgaccgcga
      301 tcgcccttat cgatcccgag accggagaca caacccggct caccgagcct gacacgatga
      361 cgggttccct cagctggtcc ccggatagcg gcaagctctt ctacgactac gagcagttcg
      421 gctaccgcat cctcgatgtc gcgtcgaaga ccgttcgcga ggtcagcgcc gatatcggct
      481 tcacccccga cagccaggcc accaatccgg ccgtctggcg cgacgagaag accgtccttg
      541 tccacggcta caccggcacc ggcagtggac tctggacgat cgacatcgag accggcacgc
      601 cgacgcagat cgtcagctgg gacgcgatcc acggtggcct gaccccgatc ccgggcagcg
      661 acaccctcct ccagtcggtc ggcgatctcg gcggcaccgc gggtgtggcc acgatcgatc
      721 cggcgacggg cgcgaagacg ctgctcttcg acgagggcac tcccttcgcc tcccatacgc
      781 cgcccgcgca gtgggagttg atctcgatcg agcgcaccgg gtacaagatc gaggcgatcc
      841 tcctcaagcc gtccaacttc gacgcgcgtc gtacgtaccc gatcatcctc gacgtccacg
      901 gcggtccgca gggcgctcac atgcagcgga tcgagccgaa ctcgcaggtc atggcctcga
      961 acggcttcct cgtcctgatg ccgaatccgc gcggctcaac gaactatggc cgcgaattct
     1021 ccgaggccgt ctatgacgac tggggcaacg aggactggaa ggatctccag gcgatcctcg
     1081 acaaggcgct cgagcttccc tatgtcgaca aggaccgcac cggcatctac ggctactcgt
     1141 acggtggcta catgacctcg tgggcgatcg gtcacaccga tcgcttcaag gcggcagtct
     1201 gcggtgcccc gccattcgac ctcgagtcga tgtacggcac gagcgatgtc tcctacgtgc
     1261 tgggtcacaa gcagtggggc ggcacaccct gggaaggacg cgaggagtac aagacgatct
     1321 cgccctccga atacatccac aacgcgacga cgccaaccct gatcgtgcat ggcgaggcgg
     1381 atgagcgatg cccgatcggc cagggcgagc agatgttcat ctccctgaag aagctcggcg
     1441 tggagaccca gttcgtccgc tatccgggcg gttcccacgg cttcgcctgg cggggtgagc
     1501 cggcacaccg gatcgacttc tacacgcgga ttctcgcatg gttcaagaag tacctcggcg
     1561 atcccgtata gcaaggcccc gatgagggac gcacgaaaac ggagcgcccg agtcgggcgc
     1621 tccgttctca tacacgccgc aggcatcaag ccgaattgac gtgcgatgcc ccatgaacaa
     1681 tctcgccgca tacatcgaaa accagaatcg tcgacccgtt ttgctggtgc tgggggtggt
     1741 agggcggggt tttatacccc gccgcaaagc ggactcgggc gaagtacgcc ccgcccccaa
     1801 ggggcaaccg tttcggtttt tgctgaagag cggcgatcgg aaccgttgcg actgggagca
     1861 caatcctcgg gcgaacgcga ggcgggacag gccgtccgga ctgcggtccg gcagcggcgg
     1921 tcccgcccta cgaacggacg ctggtccgct gcgcaccccc cacggcgacg ccggccgcga
     1981 cctggcagaa gcaccgggtc acgacatcga cgcctggggc gggatatcga actgcgttcg
     2041 atgggtcaga ccagctgact cactacgggg aggaatcggt cgcgcgcagg gcagacccgc
     2101 cttcggcgct agtcggcgac ggcttccatc gtggatgcct gcgcgaacgc ctgcaggaaa
     2161 tgcgtcggcg gctgcgcgcc ggagaccgcg agcttgccgt cgatgatgaa gaacggcacg
     2221 ccggtgatcc ccgcctgctg caccgagccg atgatcgcca gcacccggtc ccgcatcgcg
     2281 tgggatcgca gcagcggctc gatcacggcg agttgttcgt ccgtgagacc ggcatcgcgg
     2341 gcgcactgga tcaggacgtc gtcctccccg atatccttgc catcctcgaa gtaggccttc
     2401 atcacggcct cgatcacgtc ggccttcttc tcctgcggcg tgagcaggat cagctcgtgg
     2461 gacggcacgg tatcgacagc cacctcgatc ttgtcgaagt tgaaggtgat cccggcctgc
     2521 ttgccgacgc ctacgacgcg atcgaacatc gtcgcgatcg agtccgcggg aatccccttg
     2581 cgggtcgtga agcgctcgcg gaagttctcc ttcgagcccg gctcgaccgg atcgaggagg
     2641 aacggcaggt attcgaattc gactggctcg tcattcgtct tctgccattc gtcgagcgcc
     2701 tgatgcaggt tgtgcttgcc gatccggcac cacgggcaga cggtgtcttc gatgatctgg
     2761 atgcgcatgt ccttggagtt ccttccggat ggatgtctga ggcgccgaat caggatgatt
     2821 acgaaacata agtatacggc ggggcgtcaa acgtgcttgg cgagctgctc gagatcgaag
     2881 tcctgctcga tccggacccg cgcctcgtca ccgatttctc cccggtcgcg ggcgttgacg
     2941 acggcctggc gggcggcctg gagcacctcg ttgtgcaggc gtaccgtctg ctcgtgatgc
     3001 tcgcgagcat gatcgtggtc ttcctccctg aagtgctgca gctcgtgcat gaagcggtac
     3061 ctcagcaccg cctggtgatc ctgcggcacc cactcctccc gctggacttc gtccaggcgc
     3121 gcgatcgccg ccttcgccgc gagcgcacgc gcgtgcttga tctccttttc caccgatcca
     3181 tcgtccgtca ctcgcagccg gcgcatcatg aacgggatca gcgatccctg gccgaggagt
     3241 gtcaccacga tgacgccgaa cgtgaggaag atcacccggt cgcggtacgg gaacggctga
     3301 ccgttgtcga gggtcaatgg caatgccagg gccgatgcca gcgacaccac gccgcgcaga
     3361 ccggaccacc cgatgaggat gacattcccg atcggcggat atggatcgcg ggcgaggatg
     3421 ccgggaatca gcaaccgggg aaggtaggcg gacgggaaga gccagacgag tcgcgccacg
     3481 atcaccaccg cgctgatcgc cagcgcctgc aggatcagcg tcgtcattga cgtcccctcg
     3541 atcgcatcga tcagatgcgg cagctggagg ccgatcagga tgaagaccag cccgttgatc
     3601 gccagcacga cggtatccca gacgatcttg ccgccgaccc gatcctccga ggagagcgcc
     3661 cgcgaggaat gccgcccgaa atagatgccg cccgcgaccg tcgccagcac acccgagaca
     3721 tggaccgcct ccgccaggac atagatcccg atcgccgcga ggagcgtgaa cgtgatcgcg
     3781 accgacgaat cggtaatcaa cgggacgatc ttgtccatca gccaggcgca ggcgaggcca
     3841 agcgcgacgc cgccaacgca gaggacgacg aaggtcatcg aggcatccag caacgaaaag
     3901 gttccgccga cgacggcggc gacgccgaac cggtagagga tcaacgcgct cgcgtcgttg
     3961 acgagactct cgccctcgag gatcgtcacc acgcggcgcg gcgcccccag ccgggcgaag
     4021 attgccgtcg ccgcaatcgc gtccggcggc gagacgatcg ccccgagcgt gatcgccacc
     4081 gaccagggca tatccggcat caacatcctc gccacccagg ccaccacgaa cgtcgtgagg
     4141 gtgacgcaac ccagcgcaag gaggccaatc gggcgcagat tcgccttgaa gtcgcgccag
     4201 ctcgtgaaat aggcggcgta gaagaggatc gggggcagga agaggaggaa gacgagatcc
     4261 ggctcgagtt cgatccgggg caggcccgga atcagcgcga ggatcatccc gccgatgaga
     4321 agcgtcatcg ggaacggcac gttgattctg gtcgccagcg tgaccagcga aacgaccgcc
     4381 agcagcagga cgatgatgac ctgaatgtcg tgatgcgtca cgcgtgtccc ccatggtggg
     4441 tcggacgaga tgggaacatg gcggcatcgc cagccgaatc gtgagccatg aagctccctt
     4501 tgtcgaactc agggatgtcg cgaagggtga gccagtgtat cgcggatcag cgtcagaagc
     4561 tcatctcctg gaagtcgagt tggcgctcca tttcacgtcg aacctcatcg ccgatctgac
     4621 cgcggtcgcg caactcgagc aacgcctcgc gtgcggcacc aagcatctgc tgccggaagc
     4681 gaatgcgcgg ctccagccct tcctcga
//
LOCUS       DASTMD010000311         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_28552, whole genome shotgun sequence.
ACCESSION   DASTMD010000311 DASTMD010000000
VERSION     DASTMD010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28552"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFQ54_04575"
     CDS             complement(<1..270)
                     /locus_tag="VFQ54_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164296.1"
                     /translation="MAVLREADESAKVAPVRRLRLYAFSALLVVLSVLLASCTPSGLV
                     DSSPTGVDSCLDGLAPTTTDTTGVFVEPDDSRDPVLDELNAARCTI"
     gene            complement(774..1547)
                     /locus_tag="VFQ54_04580"
     CDS             complement(774..1547)
                     /locus_tag="VFQ54_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164297.1"
                     /translation="MTQAILQVAVASSVEEAIRVLIPFMRDEAGTVVLLPSSLDREDR
                     VCAIVAAECPAKVVSPLSAVRWALSSPRSRAIDLDRPDVLVPSVTIPEAVLDASRRFV
                     VCDLDAGDVARSGPFILDLLAHYLSPRDRLRILASGSRGRATAEVNLAVAIDGVIVCG
                     TFGSVHLAVWTEDRIAGELVALALAERKLDVMNRSFTGPWEDAVVQRATELDLGVRIP
                     RDIEIVIAGDGDRSGPAGLLEHIRDRLGITSRPGEWAGL"
     gene            1668..>2533
                     /locus_tag="VFQ54_04585"
     CDS             1668..>2533
                     /locus_tag="VFQ54_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164298.1"
                     /translation="MTRLCRRLFLGLLLVFLAANPFAGGALAQPATPSQPTQPTMGPG
                     SDDYAYAKTASVTVGDAPTGATIFTPDGASPAQLDAMPIVLFIPGFLASNPETYGRWI
                     DHLVRRGTIVLFPNYQTANLLAVPPETYPANLLAGVRSGVEYLTTLGIHDVRARSLHV
                     VGHSLGAVLAVSYAQNAARLKFPRVATLSVIEPGGCGTCGTFPDITVPIDLNVQLPAD
                     ILAQLVVGNDDTTVGDADARKLWPLFSDIPPRNRDYVTVRSDRHGKPPLLADHAMVGT
                     GGGPNGEEDAMD"
BASE COUNT          498 a          865 c          764 g          406 t
ORIGIN      
        1 gatcgtgcag cgggccgcat tcagctcatc aaggacagga tcgcgggaat catccggttc
       61 gacgaagaca ccggtcgtat cggtcgtcgt tggcgccagg ccgtcgagac aggagtcgac
      121 gccggtcggg gacgaatcga cgagacccga aggcgtacac gatgcaagga gaaccgagag
      181 cacgacgagc agcgcggaga acgcgtaaag ccgcaaccgg cggaccgggg cgacctttgc
      241 cgattcgtct gcctcgcgga gaacggccac atatatcctc ggctctcgga cggacggtgc
      301 cggcagcacg ccacgcgatt acacgccgga ccatcacgcc ggggagccgg caaattcatt
      361 tacattaatc aatctgtgcc accgagtcac ggcgacaggc tcacacccat acctcgtcta
      421 tcgcgttccc cgaaattcgc gtccgcccgt ccgacaggat cgagcggctg tcggcccggt
      481 atttaccgga ctcgttgctg cggactgttc gcgcgaaccg gaaccggcac gggctctggc
      541 gccggggcca acgaggggaa actctttgcc aaccactcaa gcaatcgctg caacaaccct
      601 gaacgcacgg ggttctccat tcattctcga ttcaatcgag caccgggatt tcgcctgccc
      661 cccagactcc gcctttcctt cgcccttgcg aaagaagccg acatcccgga aaagttactg
      721 tatcaattgt agcgacccgc aattcgcttg tcaccatgtg tcaacgatgc aagctacagg
      781 ccagcccatt caccgggtcg cgacgttatt cccagtcggt cgcggatatg ttccagcaaa
      841 ccagccggcc ccgaccggtc cccatctccc gcgatgacga tctcgatatc gcggggaatg
      901 cgcacgccga ggtcgagttc ggtcgcgcgc tggacgacgg catcttccca ggggcccgtg
      961 aaggagcggt tcatcacgtc gagctttcgc tcggccagcg cgagcgccac cagctcgccg
     1021 gcgatccggt cctcggtcca gacggcgagg tgaacgctgc caaaggtccc acagacgatc
     1081 acaccgtcga tcgccaccgc cagattcacc tcagccgtcg ccctcccgcg ggatccggac
     1141 gcgaggatcc gcaggcgatc gcgcggcgag agatagtgag cgaggagatc gagaatgaac
     1201 ggaccggagc gggcgacatc gcccgcgtca agatcgcaga cgacaaaccg gcgagaagcg
     1261 tcgagaacgg cttcgggaat cgtcaccgaa ggaaccagaa catccggacg gtccagatcg
     1321 atcgcgcggg agcgggggga tgacagcgcc catctcaccg cggagagcgg cgacaccacc
     1381 ttcgccgggc actcggcagc aacgatcgca cagacccgat cttcccgatc cagcgacgag
     1441 ggaagcagca cgaccgtccc ggcttcgtcc cgcatgaaag ggatgagcac gcgaatggcc
     1501 tcttcgacgg aactggcgac cgccacctgc aggattgcct gggtcaacgc cactccttcg
     1561 acgacgatcg cgcttcgacc gcaacttctt ccttgatgca tccgaacgaa ctctaggata
     1621 ctcggaggag gcccgcccgc ttccagggac ttcgtcaagg atgctccatg actcgattgt
     1681 gcagacgact gttccttgga ttgcttctcg tcttcctcgc cgcgaacccg ttcgccggcg
     1741 gcgcgctcgc ccagcccgcg acaccatccc agccgaccca gccgacgatg gggccgggaa
     1801 gtgacgacta tgcctatgcg aagacggcga gcgtcaccgt aggtgatgcg ccgaccgggg
     1861 cgacgatctt tacaccggac ggcgccagcc cggcgcagct cgacgcgatg ccgatcgtcc
     1921 tcttcatccc cggctttctc gcgtccaacc ccgagacgta cggtcgatgg atcgatcatc
     1981 tcgtgcggcg gggcacgatc gtgctcttcc ccaattacca gacggcaaat ctgctggccg
     2041 ttccgccgga aacctatccg gcgaatctcc tggccggggt gaggtcgggg gtggagtatc
     2101 tcaccacgct cggtatccac gatgtccgcg cgaggtcgct ccacgtcgtc gggcactcac
     2161 tcggggccgt gctcgccgtg agctacgccc agaatgcggc gcgactgaag ttcccccgcg
     2221 tcgcgaccct ctcggtcatc gagccgggtg gctgcgggac ctgcggaacc ttccccgaca
     2281 tcaccgtccc catcgatctg aacgtccagc ttccggcgga tatcctcgcc cagctcgtag
     2341 tcggcaacga tgacaccacg gtcggcgatg ccgacgcgcg caagctctgg ccactcttca
     2401 gcgacatccc gccccggaac cgggactacg tcacggtcag gagcgatcgg cacggcaagc
     2461 cgcccttgct ggccgatcac gcgatggtcg gtacgggcgg cggccccaat ggcgaagaag
     2521 acgcgatgga ctg
//
LOCUS       DASTMD010000312         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_741827, whole genome shotgun sequence.
ACCESSION   DASTMD010000312 DASTMD010000000
VERSION     DASTMD010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741827"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VFQ54_04590"
     CDS             complement(<1..1004)
                     /locus_tag="VFQ54_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU0164299.1"
                     /translation="MPIVNLGILAHVDAGKTSLTERILFETGVIRSVGSVDGGDTQTD
                     TLELERERGITIQSALVSFHLGNRKINLIDTPGHADFVAEVERALLALDAVVIVVSAV
                     EGIQPQTRRLVRIVRSRGLPMLIFINKIDRAGARDRELLAEFRARLGLRVFAMAESAG
                     SGSTAATVSARDLKDPIVQHELVDVLSESSDELVAAFLDRGTDPSRRMIRRVMHEQIR
                     TGELVPVYCGSAILGVGTDLLLHGVERLLPPAPECSEAPLEGVIFKIQRTPAHEKLVY
                     LRIRGGTIGRRDRVNFGRTTADGDSIHYEARVTAVDWFDRGNAMTVGRVEAGEIVRIH
                     G"
     gene            complement(1132..1788)
                     /locus_tag="VFQ54_04595"
     CDS             complement(1132..1788)
                     /locus_tag="VFQ54_04595"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU0164300.1"
                     /translation="MTNHTHFPDAEWKHGDVVALRYIRNHPADSIVPARVVEDEPSYV
                     ALYYGPGSPRVVHATVEGEPLPREIPFLEREKLIGGLRRITWTDRHVLQIQAPDRMSA
                     VLLWWDENWQFLGYYVNLQASLFRTTFGFDTADYLLDIVVSPDLAWEWKDEEEFALAR
                     LHDILAPELADAIRAEGERAIGDIEARRWPFDGSFESWRPDPSWSIPDLPQGWDQDLY
                     "
     gene            complement(1785..2060)
                     /locus_tag="VFQ54_04600"
     CDS             complement(1785..2060)
                     /locus_tag="VFQ54_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164301.1"
                     /translation="MDGRAVGTRGHPTRFTRQVGIWAWKDEDEFGEALTFGRLSPEQE
                     AQVRGEVQRMIPAIESRAWPFDGSFENWRPDPAWPVPVMPDNWNEDE"
BASE COUNT          397 a          711 c          672 g          378 t
ORIGIN      
        1 ccgtggatgc gcacgatctc gccagcctcc acacgcccga ccgtcatcgc gttgccacga
       61 tcgaaccagt cgaccgccgt cacccgggcc tcgtagtgaa tcgaatcgcc gtccgcggtc
      121 gtccgcccga agttcacgcg atcgcgacga ccgatcgtgc cgccacgaat ccggagatag
      181 acgagcttct catgtgcagg agtgcgttga atcttgaaga tcacgccttc gagcggcgcc
      241 tcactgcact ccggggcggg cggcagcagt cgttcgacgc cgtggaggag gagatcggtc
      301 ccgaccccga ggatcgccga cccgcagtag accggaacga gttcccccgt gcggatctgc
      361 tcgtgcatca cgcgacgaat catccggcgc gacgggtctg tgccacgatc gagaaacgcc
      421 gcgaccagtt cgtcgctcga ttcggagaga acatcgacaa gctcatgctg gacgatcgga
      481 tccttgaggt cgcgcgccga gaccgtcgcc gcggtggatc ccgatcccgc cgactccgcc
      541 atcgcgaaga cgcgcagtcc gagccgggcg cggaactcgg ccagaagctc gcgatcgcgg
      601 gcgccggcgc ggtcgatctt gttgatgaag atcagcatcg ggagaccgcg cgagcggacg
      661 atccggacca ggcgtcgcgt ctgcggctgg attccctcga cggccgagac gacgatcacg
      721 accgcatcga gcgcgagcaa cgcccgctcg acctcggcaa cgaagtcggc atgaccgggg
      781 gtatcgatca gattgatctt gcgattgcca agatgaaacg agaccagcgc ggactggatg
      841 gtaatgccgc gttctcgctc gagttcgagc gtgtcagtct gggtgtcgcc gccatcgacg
      901 ctcccgacgg agcggatgac gccggtttca aagaggatac gttcggtaag actcgtcttt
      961 ccggcgtcga catgcgccag gataccgagg ttgacgatcg gcatgcagca gggttccttc
     1021 atcgagaatc atgatggtca tgggatttct cgagagttgg cgcattggaa cctgcctttc
     1081 aaagcctgtc attaccgaaa gtcaccgccc catcctagcg ggcggctgat cctaatacaa
     1141 gtcctggtcc cacccctggg gcaggtcggg aatgctccac gacggatcgg gacgccagga
     1201 ttcgaagctg ccatcgaacg gccatcgccg cgcctcgata tcaccgatcg cgcgctcccc
     1261 ttccgcccgg atcgcgtcag ccagctcggg ggcgaggatg tcgtgcaggc gcgcgagggc
     1321 aaactcctcc tcatccttcc attcccaggc caggtcaggt gagacgacga tgtcgagcag
     1381 atagtccgcc gtgtcgaagc cgaaggtggt ccggaacagc gacgcctgga gatttacgta
     1441 gtaaccgaga aactgccagt tctcgtccca ccagagcagc acggcgctca tccggtccgg
     1501 cgcctggatc tgaaggacgt gccgatccgt ccaggtgatc cggcgcagcc cgccaatcag
     1561 cttctctcgc tcgaggaagg gaatctcccg gggcagcggc tcaccctcga cggttgcatg
     1621 gaccactcgc ggactgcctg ggccgtagta gagcgccacg tacgacggct cgtcctccac
     1681 gacacgcgcg ggcacgatcg aatcggcggg atggttccgg atgtatcgca gggcgacgac
     1741 atcgccgtgc ttccattcgg cgtccgggaa gtgggtgtga tttgtcattc gtcctcattc
     1801 cagttgtcag gcatcacggg aaccggccac gccgggtcgg gtcgccagtt ctcgaacgat
     1861 ccgtcgaacg gccaggcgcg ggattcgatc gccggaatca tccgctgcac ctctccacgc
     1921 acctgagcct cttgctccgg agacaagcgt ccgaaggtca gcgcctcgcc gaactcatcc
     1981 tcatccttcc aggcccagat cccaacctgt cgcgtgaacc tggtggggtg gccgcgggtt
     2041 ccgaccgcac gtccgtccat cacctgtctc gtgccgtcat gaccgttgga agaaccgtag
     2101 acgcggccct tgtggccgcc ggctgcgccg atccgttctc tcagggagcg ctatccgt
//
LOCUS       DASTMD010000313         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_117099, whole genome shotgun sequence.
ACCESSION   DASTMD010000313 DASTMD010000000
VERSION     DASTMD010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117099"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1174)
                     /locus_tag="VFQ54_04605"
     CDS             complement(158..1174)
                     /locus_tag="VFQ54_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164302.1"
                     /translation="MKKMTYSRRAVVAASASLGALAAGTGRGIAQTPVASPAASPVTR
                     DGAWSFIDDRGVTSSLPEVPMKIIAQTSSAAALWDFGIEPVGFFGPVDPDNPTGFAQV
                     GSIDLAKSENLGDYGDLDLEKVVALGAQLYVDLDRGGGTLWYLDADAQKKLEAICATV
                     GLKAYEVSVLDIVKRYGDLAFSLGVDPATPDLVAATATHATAEAAFKDAVAAADGLKV
                     VAISGGPDAGSVYIWNGEMIPDLIYFRDLGMDYIDINAEAGASNLQIALEQIGEYPAD
                     LILFDFRDDISVLSGNPVWESLPAVKAGQVGLWYGAFPYSYQELSRVLDLMTAQVAKA
                     KVIL"
BASE COUNT          299 a          548 c          473 g          277 t
ORIGIN      
        1 gagcgcgatc tcatcgtcgc ccgcatctat cgcgacgaga tcgcggcgca tcatctcccc
       61 acgatcgaga tcgacggtga ggcactgccg gaagagatcg cggcacgcgt cagccgtcag
      121 ttcacgacgt tggcgggcga cgctccgggt gtgtcgatca gagaatgacc ttcgccttcg
      181 caacctgcgc cgtcatcaga tcgagcacgc gggagagttc ctggtaggaa taggggaacg
      241 ccccgtacca gagaccaacc tgccccgcct tcaccgccgg caggctttcc cagaccggat
      301 tgccggagag gacggagatg tcgtcgcgga agtcgaagag aatgagatcc gcgggatatt
      361 ccccaatctg ctcgagtgcg atctggagat tgctcgctcc ggcctcggcg ttgatatcga
      421 tgtagtccat ccccaggtcg cggaagtaga tcaggtcggg gatcatctcg ccattccaga
      481 tgtagacgct ccccgcatcc ggtccaccac tgatcgccac caccttgagc ccgtcggccg
      541 cggcaacggc atccttgaac gccgcctcgg ccgtcgcatg cgtcgcggtc gccgcgacca
      601 gatccggcgt ggcgggatcg acgccaagcg agaacgcgag atccccatat cgcttcacga
      661 tgtcgagcac ggagacctcg tacgccttca ggccgaccgt cgcgcagatt gcctccagct
      721 tcttctgggc atcggcatcg agataccaga gggttccacc accccgatcg agatcgacgt
      781 agagttgcgc gccgagcgcc acgaccttct cgagatcgag atccccgtag tcaccgagat
      841 tctccgattt cgcgagatcg atgctgccaa cctgggcgaa tcccgtcgga ttgtcaggat
      901 cgaccggacc gaagaacccc accggttcga tcccgaaatc ccagagcgcg gctgcggagc
      961 tcgtctgcgc gatgatcttc atcgggactt ccggcaacga agacgtgacg ccgcgatcgt
     1021 cgatgaagct ccacgctccg tcgcgcgtca cgggagatgc cgccggcgac gcgaccggag
     1081 tctgcgcgat ccccctcccc gtacctgcgg ccaatgcgcc caggctcgcc gacgctgcca
     1141 ccactgctcg ccgcgaataa gtcatcttct tcatgcgtct cgttcccctc ccgcggttca
     1201 ggcgctcgat cgtcactgcg accagtgacc ttccggacgg tcaagctacc cgtccccata
     1261 tccgaccata tcagtctgct ttaagacagg ggatcgcaca aggcgcatcg tcgacgaacg
     1321 ccaccgtcca gatcgaaccg cagcggcagg ctattggagt cacgcccgga gtgcgctatc
     1381 gtgcgctcgt tctcacaatg cacgtcaccg cccggcagcc gaacctggcc gctggcttcg
     1441 agattcatca aggaagggat gctgatgacg cgtcgcatga ttcaggtcgg gaccggcggc
     1501 atgggtggcg cctggtgccg cacgatcctc cccccggaaa tcgccgccgg caccgtcgag
     1561 gtcgtcgcgg cggtggacgt caacccggcc gcactca
//
LOCUS       DASTMD010000314         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3153643, whole genome shotgun sequence.
ACCESSION   DASTMD010000314 DASTMD010000000
VERSION     DASTMD010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3153643"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(552..>2087)
                     /locus_tag="VFQ54_04610"
     CDS             complement(552..>2087)
                     /locus_tag="VFQ54_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0164303.1"
                     /translation="LYEGEARDGAILVTAGDAIGATPPISAFFDDAPTIQMMNALGFD
                     ADGIGNHNFDVSSDFFVNTIMPQANYPYLSSNLVLSANSQPVAIGTPGATPNPVTGTP
                     WSPSTTFEFNGVTLGLVGFSNDDIPTLTRPGALGPFTLSDPTTAVSAEAARLREQGVP
                     VVVAMGHMGTVAGDFNNPEGPGIDLADNVTGVDAVIGDHTDRETITTRANGVLYSEVL
                     SKGVMFNRVRVVVDTTTNTVVYKTADHHRPWTIGVTPDPTIQAALDQLNTNLAPILGE
                     VVGSSTTPILHADSCGTDNGRTCESIEGDVVTDAMRLTYGTDFAITNSGGLRADMTCP
                     TEDNPDDFCGADVAPNSITRGQVLGVLPFGNVAVTLDITGAELKAMLEAGIAQMPNVG
                     GGFPQVSGLCFTYDITKPAGERVTSVVRQEEDGSCTGEAVDLTEKSTYTLASNDFTIA
                     GGDSYPDLSAKATTREVLDEIVAAYVGGASPFAVPGAPLEPKLDGRINCTGEGCPVPV
                     DKP"
BASE COUNT          337 a          663 c          709 g          378 t
ORIGIN      
        1 ttcctcgcct gggagcagga cggcgctccc ctggccggca ccgatggccc gctccgcctc
       61 gtggttcctg gcgacgcgaa gggcggtcgc tatgtgaccg gtgtcgtctc gatcgaagtc
      121 ctgcacgtcg gcgaggccgc cgcgactcct gccgcgtcct gacgcgacat cattgagatc
      181 accgcaagtg agaagaggct ccccggctgg ggagcctctt ccgcgtccaa cgcatgccgg
      241 gagtcggatc gtcgatcggg agggatttcg tgtgggaaca cgagggcgaa cctggtgttc
      301 gcccgggact acgtcccatg cgcgatgccg cgcccgtctc ctcgtttcgg taatcgcgtc
      361 gtgaggagaa cacgtgatgc cgacgcgatc cctcgacagg ctcgggagga cgtgttttgg
      421 cgggagacgc agtgtgggga gcatgggatt accgacgcaa ttccgcgggc ttgggatggc
      481 cccgaaagag aagcgggatg gctgctggag ccatcccgct tcttgtgatt cgcctcggat
      541 tcaggccagc cttacggctt gtcaaccggc accgggcatc cttccccggt gcagttgatg
      601 cggccatcga gcttcggctc gagcggcgcg cccgggaccg cgaacgggga tgccccgccg
      661 acgtaggctg cgacgatctc gtcgaggacc tcgcgcgtcg ttgccttcgc cgagaggtcc
      721 ggatagctgt cgccgccggc gatcgtgaag tcgttcgatg ccagcgtgta ggtcgacttc
      781 tccgtcaggt cgacggcctc gccggtgcat gagccatctt cctcctgccg gacgacgctc
      841 gtcacccgct caccggcggg cttcgtgatg tcgtaggtga agcagaggcc ggagacctgc
      901 gggaaaccac caccgacatt cggcatctgg gcaataccgg cttccagcat cgccttcagc
      961 tccgcgccag tgatgtcgag cgtcacggcg acgttgccga acggcagcac gccgagcacc
     1021 tgaccgcgcg tgatgctgtt cggagcgaca tccgcgccac agaagtcgtc cggattgtct
     1081 tccgtcgggc aggtcatgtc cgcgcgaaga ccaccggagt tcgtgatcgc gaagtcggtg
     1141 ccataggtga ggcgcatcgc gtcggtgacg acatcaccct cgatcgactc gcaggtgcgg
     1201 ccgttgtcgg tgccgcagga atcggcgtgg aggatcggtg tcgtcgagga accaacgacc
     1261 tcgccgagaa tcggcgcgag attcgtattg agctgatcga gcgccgcctg gatcgtcgga
     1321 tccggcgtca ccccgatcgt ccacggccga tggtggtcgg ccgtcttgta gacgacggta
     1381 ttcgtcgtcg tatcgaccac gacgcgaacg cgattgaaca tcacgccctt gctcagtact
     1441 tcgctgtaga ggacgccgtt cgcgcgcgtg gtgatcgtct cgcgatccgt gtgatcgccg
     1501 atcaccgcat cgaccccggt cacgttgtcg gcgaggtcga tccccggtcc ctcgggattg
     1561 ttgaagtcac cggcgacggt tcccatgtgt cccatcgcca cgaccaccgg cacgccctgc
     1621 tcgcgcagcc gggcggcctc ggcgctcacg gcggtcgttg ggtcggagag cgtgaaggga
     1681 ccgagcgcac ccggtcgcgt cagcgtcggg atgtcatcgt tggagaagcc gacgagaccg
     1741 agcgtcacgc cgttgaactc gaaggtcgtc gagggcgacc acggggtgcc ggtcaccggg
     1801 ttcggcgtcg cgcccggcgt cccgatcgcc accggctgcg aattcgccga gagcacgaga
     1861 ttcgacgaga ggtagggata gttcgcctgc ggcatgatcg tgttgacgaa gaaatcggaa
     1921 ctcacgtcga agttgtgatt gccgatcccg tccgcgtcga agccgagcgc gttcatcatc
     1981 tggatcgtcg gcgcatcgtc gaagaaggcg gagatcggcg gtgtcgcgcc gatcgcatcg
     2041 ccggccgtga cgaggatcgc accgtcgcgg gcttcaccct cgtagag
//
LOCUS       DASTMD010000315         1735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_921253, whole genome shotgun sequence.
ACCESSION   DASTMD010000315 DASTMD010000000
VERSION     DASTMD010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921253"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VFQ54_04615"
     CDS             <1..1324
                     /locus_tag="VFQ54_04615"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEU0164304.1"
                     /translation="LFFCMDDEVLLDGPAGTGKSLACLKKLDRNAIKYPGSRQLIVRK
                     TRESLTQTGQVTFENHVIVPGGLVRFHTTQQAYLYPNGSKIVVSGLDRNSKVMSAEYD
                     TVYVQEATEIEEEDLEALTTRLRNGVIPHQQLIADCNPNTEKHWLNQRCIAGKMTRLL
                     SRHEDNPYLWDGRAETWTPQGEVYIGKLERLSGVRYLRLRKGLWVAAEGQVYEAWDPS
                     KHLIGPDDLEELGLLVDGQVNRLVTRQVVAGVDWGYTNPGVISVFGADYDYRLFQFAE
                     LYMTRKTIDWWIEQAIVLRDEFGIDAFICDPSEPGYIQQFINAGLNAVSAKNDILPGI
                     TKVMERLKPAGDDRRRFFLNRSGLRMPDPELVDKKLPICTADEMTSYVWPTKTGVRAA
                     KERPEDHDNHGLDTARYVVAYYDLGDDGYSEDTELALNEMLAYQAGIA"
     gene            1348..>1735
                     /locus_tag="VFQ54_04620"
     CDS             1348..>1735
                     /locus_tag="VFQ54_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164305.1"
                     /translation="MSALEAWRSYGFASELTYMQSRYDELWSLYTGEAFANALRQSAF
                     RTNRQVYRNTRLIWKHVEGVVDWYVGTVYQGDLSTDGARLPDGTYGAIPIHPQGLKAT
                     DEKNLRTAIGELFDAWNYKQQMSIGVM"
BASE COUNT          352 a          555 c          524 g          304 t
ORIGIN      
        1 gctcttcttc tgcatggatg acgaggtcct cctcgacggc cccgccggca ccggcaagtc
       61 gctcgcttgc ctcaagaagc tcgatcgcaa cgcgatcaag tacccggggt cacggcagct
      121 catcgtgcgc aagacgcgcg agtcacttac ccagaccggc caggtgacgt tcgagaacca
      181 tgtcatcgtg cccggcgggc tggtccgatt ccacacgacc cagcaagcct acctctaccc
      241 caatggctcc aagatcgtcg tcagcggtct cgatcgcaac agcaaggtca tgtcggccga
      301 gtacgacacg gtctacgtcc aggaagcgac cgagatcgag gaggaggacc tcgaagcact
      361 cacgacgcgt ctccgtaacg gcgtgattcc gcaccagcaa ctgatcgccg actgcaaccc
      421 caatacggag aagcattggc tcaaccagcg gtgcatcgcc ggcaagatga cgcgcctgct
      481 ctcccgtcac gaagacaatc cgtatctctg ggatgggcga gcggaaacgt ggacgcccca
      541 gggtgaggtc tacatcggga agttggagcg gctcagcggt gtgcggtatc tgcgcctgcg
      601 caaggggctc tgggtcgccg cggagggcca ggtctacgaa gcctgggatc cttcgaagca
      661 tctcatcggc cccgacgatc tcgaggaact cggcctgttg gtcgatggtc aggtcaatcg
      721 cctggtgacg cgccaggtcg tggccggtgt cgactgggga tacacgaatc ccggggtcat
      781 cagcgtcttc ggcgcggact acgactatcg cctgttccag ttcgccgagc tctacatgac
      841 gcgaaagacg atcgactggt ggatcgagca ggcgatcgtc cttcgcgacg agttcggcat
      901 cgacgccttc atctgcgatc cctctgaacc cggctatatc cagcagttca tcaatgccgg
      961 gctcaatgcc gtctccgcga agaacgacat cctgcccggc atcacgaagg tgatggagcg
     1021 gctgaagccg gcgggcgatg accgccggcg gttctttctc aaccgctccg ggctacgcat
     1081 gccggatccc gaactcgtcg acaagaaact gccgatctgc accgcggacg agatgacctc
     1141 ctacgtctgg ccgacgaaga cgggcgtgcg tgcggcaaag gagcgtcccg aggaccacga
     1201 caaccacggc ctcgacactg cgcgctatgt cgtcgcctat tacgaccttg gagatgacgg
     1261 ctactccgag gacaccgaac tcgcgctgaa cgaaatgctg gcctatcagg cggggatcgc
     1321 atagccatga gcctgcgggg acgattcatg tctgcactcg aagcctggcg atcgtatgga
     1381 ttcgcctccg aacttaccta catgcagagc cgctatgacg agctctggtc gctctacacc
     1441 ggggaggcgt tcgccaacgc cctgcgacag tcggccttcc ggaccaaccg gcaggtctac
     1501 cgcaacaccc gcctgatctg gaagcacgtc gagggcgtcg tcgactggta tgtcggaacg
     1561 gtctatcagg gcgatctcag caccgatggt gctcgccttc ccgatggcac ctatggcgcg
     1621 atcccgatcc atccgcaggg gctcaaggct accgacgaga agaacctccg gacggcgatc
     1681 ggcgagcttt ttgacgcctg gaactacaag cagcagatga gcatcggcgt catgt
//
LOCUS       DASTMD010000316         3297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1101227, whole genome shotgun sequence.
ACCESSION   DASTMD010000316 DASTMD010000000
VERSION     DASTMD010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101227"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..550)
                     /gene="rplI"
                     /locus_tag="VFQ54_04625"
     CDS             complement(35..550)
                     /gene="rplI"
                     /locus_tag="VFQ54_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU0164306.1"
                     /translation="MKIVLRQDVPKLGESGSIQTVANGYARNYLIPQGMAVPATEGEL
                     KVAQHNLAVKERKVLRQEEQQKSLADRISGQRLTFVAKAGDTGRLFGSITAADVAAQL
                     SSKVGEEIDRRKIVLEDALRTVGEHPVSVHLVGKLRPQITIVIEAENDGSTESPDEEA
                     VEVVAAEIDGE"
     gene            complement(689..1435)
                     /gene="rlmB"
                     /locus_tag="VFQ54_04630"
     CDS             complement(689..1435)
                     /gene="rlmB"
                     /locus_tag="VFQ54_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU0164307.1"
                     /translation="MIVRGDPLYGRNAVLESLRAGNRSTSHLWIAEGLRPDERLDEIT
                     ERATALAIPIDTVPRPMLDDLTQGLNHQGVLLEVGDYRYNDLDDILERPGTVLVLDHL
                     QDPQNFGTLLRASDAAGIAGVVIAADRSVSVTPAVVNASAGAVEHILIAQVANIARAL
                     EKIEDSGRWIVGLDDGEDAQDLFSTDVPTPVALVVGAEGSGISAGIRRRCQMILSLPM
                     LGQIESLNAATAGSIAIYDMLRRERTAEAF"
     gene            complement(1695..3104)
                     /gene="cysS"
                     /locus_tag="VFQ54_04635"
     CDS             complement(1695..3104)
                     /gene="cysS"
                     /locus_tag="VFQ54_04635"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU0164308.1"
                     /translation="MSETTASQLQIYSTLSRTKEPFVTIEPGKVRMYVCGVTVYDDAH
                     IGHGMSAIIFDVIRRYLTYSGYEVNFARNFTDIDDKIINRANQEETSPDELTSRLIAD
                     WTREIEELNIVPATITPRATTEIPAIVEMIEGLIEKGYAYQSAGDVYFRVRSFPGYGK
                     LSHRKIDELVSGARIEIAEIKEDPLDFALWKAAKPGEPSWESPWSAGRPGWHIECSAM
                     CTHHLGGLVDIHGGGSDLIFPHHENEIAQSEAYLGEEPYARFWLHNGMLRLDGEKMSK
                     SLGNVVRLKDLLARGRGAAFRLMVLQSHYRQPVNYTEDALQAAENGLSRLRSAISAVG
                     TGEGRDVDLETIAAKSRTDFRTAMDDDFDTASALAAVFDLGRAINRVAATGAGAASVA
                     TAQDVLRELTDVLGLDLQVPADEVPSDAAPFIDLLLSIRQDLRAAKQFAIADSIRNQL
                     TELGVTLEDTATGTIWKKS"
BASE COUNT          558 a         1023 c         1092 g          624 t
ORIGIN      
        1 gaacatgcga tgaaacgggt cgagcgggaa ctggttactc gccgtcgatc tcggcggcga
       61 caacctcgac agcttcctcg tccggggact cggtcgagcc gtcattctcc gcctcgatca
      121 cgatcgtgat ctgcgggcga agcttgccaa cgagatgcac ggaaaccggg tgctcgccaa
      181 cggtacgaag agcgtcctcg aggacgatct tgcggcgatc gatttcctcg ccgaccttcg
      241 acgagagctg cgcggcaaca tcagccgcgg tgatcgatcc gaacaggcga ccggtgtcgc
      301 cggccttggc gacgaaggtc agccgctgac cgctgatgcg atcggcaagc gacttctgct
      361 gctcttcctg gcggaggacc ttgcgctcct tcaccgccag gttgtgctgg gcaaccttga
      421 gctcaccctc ggtcgcggga acagccatcc cctgcggaat caggtaatta cgtgcatagc
      481 cattcgccac tgtctgaatg gagcccgact caccgagctt cggcacgtcc tggcgaagta
      541 cgatcttcat ttgcgagtac cttccaacct gtcaaagtca tgcgtgaaac cagcacaaaa
      601 atccacaccg gcgccagcat acggtcgaac cagtgcggac agtgcattag cgaatccatt
      661 ctacggtgtc ggcagggcca aacgcaaatc agaatgcttc cgccgtgcgc tcccgacgga
      721 gcatgtcgta gattgcgatc gatcccgcgg ttgccgcatt cagcgattcg atctgcccga
      781 gcatcggcag gctcaggatc atctggcacc ggcggcgaat gcccgccgaa atccccgatc
      841 cttccgcccc gacgaccagt gcaaccggag tcggcacgtc cgtcgagaag aggtcctggg
      901 catcctcgcc gtcgtcgagc ccgacgatcc agcgaccgga atcctcgatc ttttcgagcg
      961 cccgtgcgat gttcgcgacc tgggcgatca ggatgtgctc gacggctccc gccgacgcgt
     1021 tgacgaccgc gggtgtgacg ctcacggaac gatcggccgc gatcacgaca ccggcgatcc
     1081 cggcggcgtc tgacgcgcgc aggagcgtcc cgaaattctg cgggtcctgc aagtggtcga
     1141 gcacgagaac cgttcccggc cgctcgagga tgtcgtcgag gtcgttgtag cgatagtcgc
     1201 caacctcgag gaggacaccc tggtggttca gcccctgggt gaggtcatcg agcatcgggc
     1261 gtggcacggt gtcgatcggg atcgccagcg cggttgcccg ctcggtgatt tcgtcgagcc
     1321 gctcgtccgg tcgcagaccc tcggcgatcc agaggtggga cgtcgagcga ttgccggcgc
     1381 gcaatgattc cagcaccgca ttccgcccgt agagcggatc gccgcggacg atgatctgcg
     1441 gcttggtctg acgcggcgga cgcgagccct gggctcgctg tcccggcggt cggcccgctc
     1501 cgacgcgatc gctggtcggt cgcggaccgg atctgccagc gggaggaccg cccggccggg
     1561 tgcctggccg cgagggacgt ccccccgggg tggcggcgtc tcgtgccggt cgttcggtgc
     1621 gatcgggccg tccgtcaccg tcacgctcgg ggcgtggcgg acgcgcgggg gaacgcgtcg
     1681 ggcggccctc gtcgttacga tttcttccag attgtccccg ttgcggtgtc ctcaagcgtt
     1741 actccaagtt ctgtgagttg attgcggatc gagtcggcga tcgcaaactg cttcgcggcg
     1801 cgcaaatcct gtcgaatgga aagaagcagg tcgatgaatg gcgccgcgtc gctcggaacc
     1861 tcgtccgctg gaacctgcag gtccagtccg aggacgtcgg tcaactcgcg aagaacgtcc
     1921 tgggcggtgg cgacgctcgc cgcaccggct ccggttgcgg caacccgatt gattgcgcga
     1981 ccgagatcga agacggcggc gagtgccgag gcagtatcga agtcgtcatc catcgcggtc
     2041 cggaaatcgg tgcgagactt cgcggcgatc gtctcgaggt cgacatcgcg gccttctccg
     2101 gtccccacgg cggagatcgc ggagcgcaag cgggagaggc cgttttccgc tgcctgaagc
     2161 gcgtcctcag tgtagttgac tggctgccgg taatgcgatt gcagcaccat caggcggaag
     2221 gccgcgccac gaccgcgtgc cagcaggtcc ttgagtcgca cgacgttgcc gagtgacttc
     2281 gacatcttct cgccgtcgag acgcagcatg ccgttgtgca gccagaagcg cgcgtatggc
     2341 tcctcgccga gataggcttc cgactgggcg atctcgttct cgtggtgcgg gaagatcaga
     2401 tcggaaccgc cgccgtggat gtcgaccaga ccgccgaggt gatgcgtgca catcgcggag
     2461 cactcgatgt gccagccggg gcgtcccgcg ctccaggggc tctcccagct cggttctccc
     2521 ggcttcgccg ctttccagag tgcgaaatcg aggggatctt ccttgatctc ggcgatttcg
     2581 attctcgcgc cggagacgag ttcgtcgatc ttgcgatgcg aaagcttgcc gtatcccggg
     2641 aagctgcgga cgcggaagta gacgtcgccg gcactctggt aggcgtagcc cttctcgatc
     2701 agtccttcga tcatctcgac gatcgccggg atctcggtcg tggcgcgcgg cgtgatcgtc
     2761 gcaggaacga tgttgagttc ctcgatttcc cgcgtccagt cggcgatcag ccgggacgtc
     2821 agctcgtccg gggaagtctc ctcctgattc gcgcggttga tgatcttgtc gtcgatgtcg
     2881 gtgaagttgc gggcgaagtt gacctcatag ccgctatagg tgaggtagcg gcggatcacg
     2941 tcgaagatga tcgccgacat tccgtgcccg atgtgagcat cgtcgtagac ggtgacgccg
     3001 cagacgtaca tgcggacctt gccgggttca atggtgacga agggctcttt cgtccggctg
     3061 agggtgctgt agatctgcag ttgactcgcg gtcgtttcac tcacggtcgg tatgctcctt
     3121 cgcgcgtggt gggcatcctg atcgcaccaa gtatggtcga cccggtgacg ccacgtcgga
     3181 ttcagtcaga agggaattcg ggcggacacg cgtggattgt cgcgtgtcga tgccaagaat
     3241 caggggagtg tcgcgcaggg gcaagcggcg ggcgcttacc cctggagaca ggcggga
//
LOCUS       DASTMD010000317         1605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_208380, whole genome shotgun sequence.
ACCESSION   DASTMD010000317 DASTMD010000000
VERSION     DASTMD010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208380"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="VFQ54_04640"
     CDS             complement(<1..1401)
                     /locus_tag="VFQ54_04640"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU0164309.1"
                     /translation="MAKRKRIPYDPPGNRKRRRGRSDGNSLRVDQMLISRRLLLTKGA
                     VVAAFAGLAGKLGYMQLAEGANYTRKAEDNIISQELIPAPRGLILDRQGRTLASNRRS
                     WAISIIPNKLPKNDAERQYVLDTLITALQLPDALVIDPNAVPLGSSDTVYTRIATMLA
                     YDTKLIPDAVKGWKAQEKNGLILITTLSIDDAARFRAASAELPGVTVMNQLEYLVENV
                     WDGDLEISVMRNVSRQVALTLEANSMYLPGVLFDDSALVRQYSGGQVMSHIVGYVQGI
                     DPIELTDIRNLDRNGKQIYQQNDLIGKAGLEVALEDHLRGKKGSRSVEKDALGVQVRV
                     VPDSTIDPVPGQNVKLTIDLELQNAAGKALADQIKRAADAKKIVNDARVAAGAKPWNT
                     PQAGSVVALDPRTGEVLAMISAPYYDNQLFISGLSSLKWTEYTSADKGKAFVNRATSE
                     LYPPGSTFKSFLASSAL"
BASE COUNT          279 a          497 c          512 g          317 t
ORIGIN      
        1 cagcgccgat gaggccagga agctcttgaa cgtcgagccc ggcggataga gctccgatgt
       61 cgcgcggttg acgaaggcct tgcccttgtc ggcgctcgtg tattccgtcc acttcaggct
      121 ggagaggccg gaaatgaaga gctggttgtc gtagtacggt gcgctgatca ttgccaggac
      181 ctcgccggtg cggggatcga gggcgacgac acttcccgcc tggggggtgt tccacggctt
      241 ggcgccggcg gcgacacggg catcgttgac gatcttcttc gcatccgccg cgcgcttgat
      301 ctgatccgcc aacgccttcc cggcggcatt ctgcaactcg agatcgatcg tcagcttcac
      361 gttctgaccg ggaaccgggt cgatcgtgct gtcgggcacg acccgaacct gtacacccag
      421 ggcgtccttc tcgaccgacc gcgatccctt cttcccacgc agatgatcct ccagcgcaac
      481 ctcgagcccc gccttgccga tcaggtcgtt ctgctggtag atctgcttgc cgttgcgatc
      541 gagattgcgg atgtcggtca gttcgatcgg atcgatgccc tgcacgtagc cgacgatgtg
      601 ggacatgacc tggccgccgg aatactggcg gacgagtgcg ctgtcgtcga acaacacacc
      661 gggcaggtac atcgagttcg cctcgagcgt cagggcaacc tgacgcgaga cgttgcgcat
      721 caccgaaatc tcgagatcgc catcccagac gttctcgacg agatactcca gctgattcat
      781 caccgtcaca cccggcaatt cggcgcttgc cgcccggaat cgcgccgcgt cgtcgatcga
      841 gagcgtcgtg atcaggatga gtccgttctt ctcctgtgct ttccagccct tcacagcgtc
      901 gggaatcagc ttcgtatcgt aggcgagcat cgtcgcgatc cgcgtgtaaa ccgtgtccga
      961 cgaccccagc ggcaccgcgt tcggatcgat gacgagggcg tccgggagct ggagcgcggt
     1021 gatgagggta tcgaggacgt actgcctttc cgcatcattc ttgggcaact tgttgggaat
     1081 gatgctgatc gcccaggagc gccggttgct ggcgagcgtc ctcccctgcc ggtcgaggat
     1141 caggccgcgc ggagccggga tcagctcctg ggagatgatg ttgtcctcgg ccttgcgcgt
     1201 gtagttggcg ccttccgcga gctgcatgta gccgagcttg ccggcgagcc cggcgaaggc
     1261 cgcgacgacc gcccccttcg tcaacagcaa cctgcgcgag atgagcatct ggtcgacacg
     1321 caggctgttg ccgtcggacc gaccgcgtcg tcgtttgcga tttccggggg gatcgtaggg
     1381 aatgcgcttg cgcttggcca tgaacgggag gtccttgtga tcgtatggat gaatgctgcc
     1441 tccgacagcc ccgattatga cgtcatccgt ccgctgggaa caagctgtga aaatgttcac
     1501 cgtgaatcac gaatccgcgc gcagcgcagc caaacgtgga caccgcccac atttggctac
     1561 agggcatcga ggccgggagc cgaggcggga cataggagtc gaggc
//
LOCUS       DASTMD010000318         1921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1367808, whole genome shotgun sequence.
ACCESSION   DASTMD010000318 DASTMD010000000
VERSION     DASTMD010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367808"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFQ54_04645"
     CDS             <1..426
                     /locus_tag="VFQ54_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEU0164310.1"
                     /translation="WVATVDAFQQWLYTDPAAADRDARDAKFVETWSRFDPGIDWSGL
                     ETERVARWYKQLHIFLYPFYYIEYAIAQLGALQVWRNALQDQEKAVADYRAALALGGS
                     QPLPALFGRAGARLIFDAEGMAELVSLIEDELTKIETPV"
     gene            complement(1010..1849)
                     /locus_tag="VFQ54_04650"
     CDS             complement(1010..1849)
                     /locus_tag="VFQ54_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164311.1"
                     /translation="MNARRLSIVTLFCLLASVFAGITPVAAQGSRSEAVTASAYCQAG
                     SKISITLRNTFGLPIVVTSVNGFAPAADLNLAFIDSNWTPTLERTIQNGDWVVLESTS
                     GSEFGGAIVVTTAGVLMPTCDGRTVGLWDDPGQLPRTDGEAQYQAIAIAAMTIGRLES
                     VRDYSALYELLHPDVRAHVPYAGISCWYEGVYGTPDDWKDSVFGTSVDDITMIDGWVW
                     PAGNVTYDNVAEVTTTQRIGTMSDSSDVRSTSHLVQSGGVWRWFFGTKVDSINQLYKT
                     CGL"
BASE COUNT          356 a          618 c          597 g          350 t
ORIGIN      
        1 tgggtcgcca ccgtcgacgc gttccagcag tggctctaca ccgatcccgc cgcggccgat
       61 cgcgatgcgc gcgacgccaa gttcgtcgaa acctggagcc gcttcgatcc gggcatcgac
      121 tggagcggtc tcgagacaga gcgggtcgcg cgctggtaca agcagctcca catcttcctc
      181 tatcccttct actacatcga gtatgcgatc gcgcagctcg gcgcgcttca ggtctggcgc
      241 aacgcattgc aggaccagga gaaggccgtc gccgactacc gcgccgcgct cgcgctcggc
      301 ggttcgcagc cgctgccggc gctcttcggt cgcgccggcg cccgcctcat cttcgacgcg
      361 gaggggatgg ccgaactcgt ctccctgatc gaagacgagc tcacgaagat cgagaccccg
      421 gtgtaacgag ccgaacgacc ggggccaggt ctggcccccg catcaatcga gttcgtaggg
      481 gcgagcctgg tgctcgcccc ctggtttcgc accccgatca gggacgaacg aagaccccgc
      541 ccggagcaat tctgggcggg gtctcgtatg gcagaagacg gtcgatatga cgcgtcctgt
      601 gtcgatcgac acgcttgcga tcgcccgcgg tttgtgcggc cggagcgtat ctccaacgct
      661 gtcgcatcaa ccctcgtcat ttcgagcttg tcgagaaatc cccgtgcgaa gccgatccgc
      721 cgcagccgga ctccggcacg aacgcgccat ccgcgttgct ctcgaaagcg ggtcgccgca
      781 ggcgacccgt ggcgaccaca agggtcgcgt ctacggttcc ctacatgtcg gtgattagga
      841 agcccagtct tggcaacgat cagatcgggc tgcccctgat gggccacagc ggatgacctg
      901 ctacgacgga gatatcggtc gtgagtacgg ggggacttct atgtcccccg tttggacgat
      961 cgttgatgac ggcgcgcctt gtgatcgcac cgtaccggcg gtatgccagc tacaacccac
     1021 aggtcttgta gagctggttg atcgagtcga cctttgtgcc gaagaaccag cgccagacgc
     1081 ctccggattg cacgaggtgg gaggtcgacc gcacgtcact ggaatcgctc atcgtgccga
     1141 tcctctgcgt ggtcgtgact tccgcgacgt tgtcgtaggt gacgttgccc gccggccaca
     1201 cccaaccgtc gatcatcgtg atgtcatcga cggacgtgcc gaagaccgaa tccttccagt
     1261 cgtccggggt gccgtagact ccctcgtacc agcacgaaat cccggcatag ggcacgtggg
     1321 cgcggacgtc aggatgcagg agctcgtaga gcgccgaata gtcgcgaacc gactcgagtc
     1381 gcccgatcgt catcgcggcg atggcgatgg cctggtactg cgcttcgcca tccgtccgcg
     1441 gcaactgacc gggatcgtcc cagagaccaa cggtgcggcc atcgcaggtc ggcatcagca
     1501 caccggctgt cgtgacgacg atcgcaccgc cgaactccga accggacgtt gattcaagca
     1561 cgacccagtc cccgttctgg atcgtccgct ccaacgttgg tgtccaattg gagtcgatga
     1621 aggcgagatt caggtcggca gccggagcga atccattcac cgatgtaacg acaatgggca
     1681 aaccgaaggt gttcctgagt gtgatgctga tcttcgatcc ggcctggcaa taggcggatg
     1741 ccgtgaccgc ttcgctgcgt gacccctggg cggcgaccgg cgttattccc gcgaatactg
     1801 acgcaagtag gcagaacaag gtgacgatcg agagtctgcg cgcgttcatc gagaactcct
     1861 gtttcttcaa agaggtggcg agaacggaaa tcgtccccta accgcagctc gcgttcagcc
     1921 g
//
LOCUS       DASTMD010000319         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_475719, whole genome shotgun sequence.
ACCESSION   DASTMD010000319 DASTMD010000000
VERSION     DASTMD010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475719"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(590..1960)
                     /locus_tag="VFQ54_04655"
     CDS             complement(590..1960)
                     /locus_tag="VFQ54_04655"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEU0164312.1"
                     /translation="MVDRETILGTPPTPEEIYAKIEAEGVRFINLQFTDVMGIVKSVT
                     IPSSIFQDVVEGGQWIDGSSIAGFTRIAESDMFLEPDLSTFAVIPWERGEFTTARVIC
                     WVHNPNGDPFPGDPRGVLYRQLEKLAALGFTYNVGPELEFFLFAKDGEGISPLPHDRG
                     GYFDLSTDLASTVRKDMVVALTEMGIDVEASHHEVAIGQHEIDFEYGAAIPTADRAVS
                     FKYILKAIAQKHGLHATFMPKPLEGINGSGMHVHQSFGFLDKSKGKNAFVDSSSPYGL
                     SEIALQFIAGQLKHARGLAAVIAPLVNSYRRLVPGFEAPVYVAWARTNRSALIRVPAI
                     RAGNLNATRIELRCPDPSCNPYLAFSAMLAAGIDGIENKLELSDPVEENLYHFTDEDL
                     KRRNIPTLPATLGEAVQELQKDEVIQAALGDHVYERLVEGQSSEWNSFRQHVSQWERD
                     RYLEIY"
     gene            complement(2046..>2305)
                     /locus_tag="VFQ54_04660"
     CDS             complement(2046..>2305)
                     /locus_tag="VFQ54_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164313.1"
                     /translation="WNGPLAFVQAARSRSIGLHNGSLVEMLRSPKGLGPRAQSILVHK
                     YQDLCENISNPHDADLWPDLVRFRIVFRYGSRVHVRGFAGW"
BASE COUNT          425 a          728 c          740 g          412 t
ORIGIN      
        1 tgatccgctg atcgtggatg gaacgatcga ggtctgggat cgtcccggca tgggtatcga
       61 actgaacgtc gagaaggcgg ccaagtacct ctccgccggt gatgccgact tctttgcatg
      121 aaccgggctc ccgctcccgt ccccacggcg cttgtcgccg tttcggatcg gcggcggcga
      181 tccgttcagg ggagcgccgc gccctatcga cgcgttgccg ggaacgggga ggcgggacat
      241 tactccatcc aaaagaccgg gcctggccct gcccaggcgg gttgctcgcc ggtctttgga
      301 ttccgcagtc ccgccccacg tacgagcgcg tatcccacgg tcatacacgc cagaatcccg
      361 cagcaaacgg atcgtgggga cagcgtcacg ttcgtagggc gggaccgccg ctgccggacc
      421 ggaggtccgg acggcctgtc ccgcctcccg ctggcggccg acccgtcccg cctcccaccg
      481 acgacgcacg gctgcacgca tgcgaaacgg ccccggcgaa gatcgccgga gccgttcgtt
      541 gagattcgtg ttactcgaga tcggtcgccc cgggaggcgg atgccgatcc tagtagatct
      601 cgaggtagcg atcgcgctcc cactgggaga cgtgctggcg gaacgagttc cactccgagg
      661 actggccctc gaccaggcgc tcgtagacgt gatcgccgag cgcggcctgg atgacctcgt
      721 ccttctgaag ctcctggacc gcctcgccga gagtcgccgg cagcgtcggg atgttccgtc
      781 gcttcaggtc ctcgtcggtg aagtggtaga ggttctcctc gaccggatcc gaaagctcga
      841 gcttgttctc gataccgtcg ataccggccg cgagcatcgc cgagaaagcg aggtacgggt
      901 tgcaggacgg atccgggcaa cgcaactcga tgcgggtcgc attgaggttc ccggcgcgga
      961 ttgccggcac gcggatcagg gcggagcggt tggtgcgcgc ccaggcgacg tagaccggag
     1021 cctcgaagcc cggtaccagg cggcggtagc tgttcaccag cggcgcgatc actgcggcga
     1081 gaccgcgagc gtgcttgagc tgaccggcga tgaactgcag cgcgatctcg gagagaccat
     1141 acggcgagct cgaatcgacg aaggcgttct tgcccttgct cttgtcgagg aagccgaagc
     1201 tctggtgcac gtgcataccg gaaccattga tgccttcgag cggcttcggc atgaacgtcg
     1261 catggagtcc atgcttctgg gcgatcgcct tcaggatgta cttgaaggaa acggcgcggt
     1321 ccgccgtcgg gatcgcggcg ccatactcga agtcgatctc gtgctggccg atcgcgacct
     1381 cgtggtggct ggcttcgaca tcgatcccca tctccgtcag cgcgacgacc atgtccttgc
     1441 ggaccgtcga ggcgaggtcg gtggaaaggt cgaagtatcc gccgcggtca tgcggcagcg
     1501 gggagatgcc ctcgccatcc ttggcgaaga ggaagaactc gagctccggg cctacgttat
     1561 aggtgaagcc gagcgccgcg agcttctcga gctgacggta gagcacgccg cgcgggtcgc
     1621 ccgggaacgg atcgccgttc gggttgtgga cccagcagat gacgcgcgcc gtggtgaact
     1681 cgccgcgctc ccatgggatg accgcgaacg tggagagatc gggctccagg aacatgtccg
     1741 actcggcgat tcgcgtgaac cccgcgatcg acgaaccgtc aatccactgc ccaccctcga
     1801 cgacgtcctg gaagatcgac gaaggaatcg tcacgctctt cacgatgccc atcacatcgg
     1861 tgaactggag gttgatgaac cggacgccct cagcctcgat cttcgcgtag atctcttccg
     1921 gtgtgggtgg ggtcccaaga atcgtttcgc gatcaaccac gtctggagtc tcctcaaacg
     1981 tcttgcctgt catcagcgcg gctgctgatg accaatgcta tccggtggga ccttagggag
     2041 gaacctcacc atccggcgaa tccgcgcaca tgtacgcgcg atccataccg gaacactata
     2101 cggaagcgta cgaggtctgg ccataggtcc gcatcatgcg gattgctgat attttcacaa
     2161 agatcttggt acttatgcac aaggatcgat tgtgcacgcg gccccaatcc cttgggcgaa
     2221 cgcaacatct ccaccagcga gccattatgt aacccaatgg aacgcgaacg ggccgcctgc
     2281 acgaatgcaa gcggcccgtt ccaca
//
LOCUS       DASTMD010000320         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2529312, whole genome shotgun sequence.
ACCESSION   DASTMD010000320 DASTMD010000000
VERSION     DASTMD010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2529312"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFQ54_04665"
     CDS             complement(<1..464)
                     /locus_tag="VFQ54_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164314.1"
                     /translation="MFARIETKWLIAIAFVLGLFLEVLDMTVLNTALPTIGRDFGSNA
                     STLQFVLTGYLVSLAIFIPASGWVADHFGTKRTFAFAIFVFTAASALAGAAQSMEWLI
                     AARILQGVGGGMLTPVGTTMLFRAFPNEERARASSVLSIPVMIAPALGPILGG"
     gene            629..1228
                     /locus_tag="VFQ54_04670"
     CDS             629..1228
                     /locus_tag="VFQ54_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0164315.1"
                     /translation="MATKTDELVGKELLLEVACDLFMEAGYDGVSMQQIAEAAHMTKG
                     SPYYHFKGKDDLFAQAFVHRTQQMHDGMVGVLVGSGDIRERLVRAVSFLLREVDAGMI
                     RFMEDCKRHLPAETMESEQAFNPEALLDIYTPIFEEAATTIGLRIPAKRAAIVFFAMQ
                     MGTLHAMHLTGELSDAPAEIERVATETVDCFLYGTAATA"
     gene            1351..2967
                     /locus_tag="VFQ54_04675"
     CDS             1351..2967
                     /locus_tag="VFQ54_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HEU0164316.1"
                     /translation="MSSLSDAVPSPLREMREWSLATSRPYARPFVDVRVTATFTSPSG
                     AVLAIEAFHDGDGVWKVRFNPGKAGMWTYAVASTPANPDFAREGSFEVTPRDTRGFLR
                     STPGTGWGFTFESGDPVFILGDTVYHLFGMAHNSSEGPAEVRAFMERRAAQGFNLLRI
                     RLPVSYFHPVDGYNVWQTTRLWPWGGSEQSPRFDQFDLPYFRTVDRVVAWAEELGIGI
                     EMIMEGWGNEFPFNRRDVFVAEWEELWLRYLVARYDAFAAVWFWTLHNEYEYYPNGNW
                     NYDAINTSCDLWAIRTGRLVREVASHGHAIAIHNGPVTPSFGKRFAADPMAIDTVMFQ
                     TWGTTGEHDAWLAAGIEETIERSLGDWPGTAVFSEYGYEFNPDLPPLMLGHQYCGPDH
                     TRRGAWRGAFRSLGVVHGFENSWGPFQILDEDQPGLPSLLHLNAFFTEVVDFAALRPA
                     DDLLEDGEDRPGYAPLALATADRSTIAVYFPAGGDAGLTIDLARHTGRWFDPRTGVIH
                     EANPGPTGRFLAPADVTAEHPDDWVLLLRR"
     gene            3167..>3523
                     /locus_tag="VFQ54_04680"
     CDS             3167..>3523
                     /locus_tag="VFQ54_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEU0164317.1"
                     /translation="MSTPLPTPKPEGYVVLGERIAELRAAGICPTCRDIAQGDVFVDQ
                     VIVTEDEQMRVVLDQFPRAHGQVIVVWKEHHEDFTTLAPVETGALFTRCTEIAFAIKI
                     GLGAEKIYLVTMCDGVP"
BASE COUNT          629 a         1119 c         1173 g          602 t
ORIGIN      
        1 ccaccgagaa tcggaccgag tgccggcgcg atcatcaccg ggatcgagag cacggacgag
       61 gcgcgggcgc gctcctcgtt cgggaaggcg cggaagagca tcgtcgtgcc gaccggggtc
      121 agcatgccac caccgacacc ctgaaggatg cgggcggcga tcagccactc catcgactgg
      181 gcggcgccgg cgagcgccga ggcggcggtg aagacgaaga tcgcgaaggc gaaggtccgc
      241 ttcgttccga agtgatcggc gacccatccc gaggccggga tgaagatggc gaggctgacc
      301 aggtaaccgg tcaggacaaa ctgcagtgtg gaggcattcg agccgaaatc gcgcccgatc
      361 gtgggcaatg ccgtgttgag caccgtcatg tccaggacct cgaggaagag ccccagaacg
      421 aaggcgatgg cgatcaacca ttttgtttct atgcgtgcga acatccgcgc taacccctgt
      481 atatcgcaac ccaaccaaac gttcgtttgg tgacctccac atactaaacg aacgtttggt
      541 ataatgcaag tgtcaggttt gaggaacctc ccgggggcac agtgaggtgc cggaatcctc
      601 gagatccatg cgcaaggaac aaaacgagat ggcaacgaaa acggacgagt tggtcggcaa
      661 ggaactgttg ctggaggtcg cgtgcgacct cttcatggag gcgggatacg acggcgtgtc
      721 gatgcagcag atagccgagg ccgctcatat gacgaaggga tcgccctact accacttcaa
      781 ggggaaggat gatctcttcg cgcaggcgtt cgtgcatcgc acgcagcaga tgcacgacgg
      841 aatggtgggg gtgctcgtcg ggagcggcga tatccgggag cggctcgtgc gtgccgtgag
      901 cttcctgctc cgggaggtcg acgccgggat gatccgcttc atggaggatt gcaagcgcca
      961 ccttcccgcg gagacgatgg agtccgagca ggcgttcaat cccgaggcgc tgctcgatat
     1021 ctacacgccg atcttcgagg aggcggcgac cacgatcggg ttgcggattc cggcgaaacg
     1081 ggcggcgatc gtcttcttcg cgatgcagat gggcacgctc cacgcgatgc atctgaccgg
     1141 agaactctcg gacgcgccgg ccgagatcga gcgggtcgcg accgagacgg tcgattgctt
     1201 cctctatggc accgccgcca cggcctgagt cgcgagcagg gcaagggcga gtcacttgcg
     1261 cgggtgtcag gtcgcacgta aggtgggaat caccgtcacg acctgatgca gcgcgagcac
     1321 gttcgcacgc cagagccaag gatgccgccg atgtcctcgt tgtccgacgc cgtcccttcc
     1381 ccgcttcgcg agatgcgcga atggtcgctg gcgacctcgc gtccctacgc gcgaccgttc
     1441 gtcgacgttc gcgtcaccgc gaccttcacg agcccgagcg gcgcggtcct cgcgatcgag
     1501 gcctttcacg atggggacgg cgtgtggaag gtccgcttca atcctggtaa ggcgggaatg
     1561 tggacctatg ccgtggcgtc gaccccggcg aatcccgatt tcgcgcggga ggggagcttc
     1621 gaggtcacgc cacgcgacac gcgcggcttc ctccgctcca cgcccgggac cggatgggga
     1681 ttcacattcg aatccggtga tcccgttttc atcctcggtg acaccgtcta ccatctcttt
     1741 ggcatggcgc acaacagttc cgagggtcct gccgaagtcc gcgccttcat ggaacgccgc
     1801 gccgcacagg gcttcaacct ccttcggatc cgcctcccgg tcagctactt ccaccccgtc
     1861 gacggctaca acgtctggca gacgacgcgg ctctggccgt ggggtggcag cgagcagtcg
     1921 ccccgcttcg accagttcga tcttccgtac ttccggaccg tcgatcgtgt cgtggcgtgg
     1981 gcggaggagc tcggaatcgg gatcgagatg atcatggagg gatggggcaa cgagtttccc
     2041 ttcaatcgtc gcgatgtctt cgtcgccgag tgggaggagc tctggctccg ctacctcgtc
     2101 gcccgttacg acgccttcgc cgcggtctgg ttctggacgc tccacaacga atacgagtac
     2161 tacccgaacg gcaactggaa ctacgacgcg atcaacacca gttgcgacct ctgggcgatc
     2221 cggaccggtc gactcgtgcg cgaggtcgcg tcgcacgggc acgcgatcgc gatccacaac
     2281 ggaccggtga cgccgtcgtt cgggaagcgg ttcgccgccg acccgatggc gatcgacacg
     2341 gtgatgttcc agacctgggg gacgaccggc gagcacgatg cctggctcgc ggcgggaatc
     2401 gaggagacga tcgaacgcag cctcggtgac tggccgggga cggcggtctt ctccgagtac
     2461 ggctacgagt tcaaccccga cctgccaccg ttgatgctcg gccaccagta ctgcggaccg
     2521 gatcacaccc ggcggggcgc ctggcggggc gcgttccgga gcctgggtgt cgtccacggc
     2581 ttcgagaact cgtgggggcc gttccagatc ctcgacgagg accagccggg cctcccctcc
     2641 ctcctccatc tgaacgcgtt cttcaccgag gtcgtcgatt tcgccgcgct tcgtccggct
     2701 gatgatctgc tcgaggatgg cgaggatcgt cctggctacg cgccgcttgc actggcgacg
     2761 gcggaccggt cgacgatcgc ggtctacttt ccggccggtg gtgatgccgg gctgacgatc
     2821 gatctcgccc gccacacggg ccgctggttc gatccgcgga cgggagtcat ccacgaggcg
     2881 aaccctggcc caaccgggcg cttcctggcg ccggcggatg tgacggcgga gcacccggac
     2941 gactgggtcc tactgcttcg ccgctgacgc cggaccgagg aggacgaggc gggacaggcc
     3001 gtccggaccc attcggcaag ctcggggcag gctctccggt ccggcagcgg cggtcccgcc
     3061 ctccgtacga ccggcctgtc gcatcccgcc tgctcgatct ttaccgtagg gctacaatcg
     3121 gcgtctgcca ttcgcgggat cgagcctgaa gaggagccag attgcgatgt cgacgccact
     3181 accaacgccg aagcccgaag gttatgtcgt gctcggcgag cgcatcgccg aattgcgtgc
     3241 ggccggaatc tgcccgacct gccgggacat cgcgcaggga gatgtcttcg tcgaccaggt
     3301 gatcgtgacc gaggacgagc agatgcgcgt cgtcctcgac cagttcccgc gcgcccacgg
     3361 acaggtgatc gttgtatgga aggagcacca cgaggacttc acgaccctcg cgccggtgga
     3421 gaccggcgcg ctcttcacgc gctgcaccga aatcgcgttc gcgatcaaga tcggtctggg
     3481 tgcggagaag atctacctgg tgaccatgtg cgacggcgtc ccg
//
LOCUS       DASTMD010000321         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2797234, whole genome shotgun sequence.
ACCESSION   DASTMD010000321 DASTMD010000000
VERSION     DASTMD010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2797234"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1057
                     /locus_tag="VFQ54_04685"
     CDS             149..1057
                     /locus_tag="VFQ54_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU0164318.1"
                     /translation="MRVFVTGASGWVGRGLVPELIAAGHTVTGLARSDAAARALRAAG
                     VEVRSGSLDDLDTLREVAASSDGVIHLAFKHDIAFAGDFAGATDADRAAIEAFGEVLA
                     GTDKPFVIASGILGVLGLPEGVVATERDGLAADAAAGEDRDVPIAGANGRVANAHLTL
                     AFADRGIRSSVVRLPPATHGGGDNGFTTTAIGLARAKGAAAYVGDGANRWPSVHRDDA
                     ARLFRLALESAPAGSVLHAVGDEGVPIREVAEVFAAHLGCPRSPSRRSRWGTISAGSV
                     ASGASTAPPRLRLPATWLDGSQPVRD"
     gene            complement(1124..1915)
                     /locus_tag="VFQ54_04690"
     CDS             complement(1124..1915)
                     /locus_tag="VFQ54_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU0164319.1"
                     /translation="MDDVFRALADRNRRLLLDRLNARNGQSLRDLCAGLEMTRQSVSK
                     HLAVLEEANLVTAVWRGREKLHYLNVEPINAIAERWINHFDRARVRALTDLKLALEGS
                     SMTDASTFVYTTYIRTSPEKLWQALTEPAFTQRYWGLAFETDWKTGSPIIWHQGDIAI
                     ANPEQKVVEANPFTRLSYTWHTFTPEWYETARGFVPDDFMERIATERRSKVTFEIEDQ
                     GGKVKLTVIHDDFDPGSVVLESVSGGWPPILSNLKTLLETGELLP"
     gene            complement(2001..>2227)
                     /locus_tag="VFQ54_04695"
     CDS             complement(2001..>2227)
                     /locus_tag="VFQ54_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164320.1"
                     /translation="DVVRETFRDWVDVIVEGLEATGVAAGLARPIAIATLAGMEGALI
                     LCRAEGSVEPLEAVATQLFRLLPGVTSEPA"
BASE COUNT          343 a          728 c          719 g          437 t
ORIGIN      
        1 gccccgccat acctgcctcc tggttcatcc cggcttgaca ttgctggtac aactgtacta
       61 caaatatttg tagtacgaac gtaccaataa tagcaaggcg acgtcaccgt gaatccatcc
      121 ggtgaacgcc cgtatcgtgg aggttcccat gcgcgttttc gttaccggtg catcaggctg
      181 ggttggtcgc gggctcgtcc ccgagctgat cgcggccggt cataccgtca ccgggctcgc
      241 ccgctccgat gccgcggccc gggccctgcg ggccgcgggc gtcgaggttc gctcgggatc
      301 gctcgatgac ctcgacaccc tccgcgaggt ggccgcgtca tccgatggcg tcatccatct
      361 cgcgttcaag catgacatcg ctttcgctgg cgatttcgcc ggagcgaccg acgccgatcg
      421 cgcggcgatc gaagccttcg gcgaggtgct cgccggcacg gacaagccgt tcgtgatcgc
      481 ctccggcatc ctcggtgtgc tcgggcttcc ggaaggagtc gtcgccaccg aacgcgacgg
      541 gctggcggca gacgccgctg ccggcgaaga tcgcgacgtc ccgatcgccg gagccaacgg
      601 gcgggtcgcc aacgctcatc tcacgctcgc gtttgccgac cgcgggattc ggtcgtcggt
      661 cgtgcggctc ccaccggcca cgcacggggg tggcgacaac gggttcacca ccaccgcgat
      721 cggcctggcg cgtgcgaagg gcgccgccgc ctacgtcggc gatggcgcca atcgctggcc
      781 gtcggtccat cgcgacgatg ccgcgcggct attccgtctc gcgctcgaat ccgcgccggc
      841 cggttctgtg ctgcatgcgg tcggcgacga gggcgtgccg atccgggagg ttgccgaggt
      901 cttcgccgca catctggggt gcccgcgatc tccgtcacgc cggagcaggt gggggactat
      961 ctcggctggc tcggtcgctt ctggggcgtc gacggccccg cctcggctca gattacccgc
     1021 gacctggttg gatggcagcc aacccgtccg ggattgatcg ccgatctcga ggagggccac
     1081 tacttcgcct gaacgctggg gaatgccctg cgccgctcct gcgctatggc aggagttcgc
     1141 cggtttcgag tagcgtcttg aggttcgaga ggattggagg ccagccaccg ctgacgctct
     1201 cgagtacgac gctgcctgga tcgaagtcat cgtggatcac ggtcagcttg accttgccac
     1261 cctggtcctc gatttcgaac gtcactttcg aacggcgctc ggtggcgatc cgctccataa
     1321 agtcgtccgg tacgaagccc cttgccgtct cgtaccattc gggggtgaat gtgtgccagg
     1381 tgtagctgag ccgcgtgaag ggattcgcct cgacgacctt ctgctccgga ttcgcgatcg
     1441 cgatgtcgcc ctggtgccag atgatcgggg agccggtttt ccagtcggtc tcgaaggcaa
     1501 gcccccaata ccgctgggtg aaggcgggct cggtgagcgc ctgccacagc ttctcgggtg
     1561 aggtcctgat gtaggtcgtg tagacgaacg tgcttgcgtc ggtcatcgag gatccttcca
     1621 atgcgagctt gagatcggtg agcgcgcgca ctcgcgcccg gtcgaagtga ttgatccacc
     1681 gttcggcgat cgcgttgatc ggttcgacgt tgaggtagtg gagcttttcc cgtccgcgcc
     1741 agacggccgt gaccagattc gcctcttcca gcactgccag atgcttgctc accgattggc
     1801 gcgtcatctc caggccagcg caaagatcgc gcaggctctg gccgttgcgt gcgttcaggc
     1861 ggtccagtaa caggcgtcga ttccgatccg cgagcgccct gaaaacgtcg tccatttcac
     1921 gtcacttcac gataggcagc cattcggatg cctattcatt atgcagtcat atggttgcat
     1981 gtcaagatcc atcgcgaatc tcaggccggc tcgctggtga cccctgggag gagccgaaac
     2041 aactgggtgg cgaccgcttc cagcggttcg accgagcctt cggcccggca cagaatcagc
     2101 gcgccttcca tcccggccag cgtcgcgatg gcgattggtc gtgcgaggcc ggcggcgacg
     2161 ccggtggctt cgagtccctc cacgatcacg tcgacccagt cgcggaaggt ctcccgcaca
     2221 acgtcga
//
LOCUS       DASTMD010000322         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2083149, whole genome shotgun sequence.
ACCESSION   DASTMD010000322 DASTMD010000000
VERSION     DASTMD010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083149"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFQ54_04700"
     CDS             complement(<1..671)
                     /locus_tag="VFQ54_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0164321.1"
                     /translation="MSDRKVQIALVGCGKIAQTHAMALSTLEEADWVAVCDRDVERAK
                     EMAAKYNVPNVFSDAAELFASGLVEAALCCTPHPAHEAVVVAAANAGIHVMCEKPIAT
                     KIDEADRMIAAADKAGIKFGVIFQRRFWPAAQRIRAAIDDGKLGNITLGLCDVRLWRG
                     PEYFGADPWRGKWATEGGGILMNQAVHSIDHFQWFMGKAVEVYGKFATLRHGAYIDVE
                     DTAVAT"
     gene            complement(734..1690)
                     /locus_tag="VFQ54_04705"
     CDS             complement(734..1690)
                     /locus_tag="VFQ54_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0164322.1"
                     /translation="MKIGFASLVGVEPMPFPELVRWAGANGLDAIEVNVGSGYRPIAG
                     APYGGHLDLAKIASEGPGELTDLLAENGVEIASLAPMVNLLTADLAKREARIAEFRLA
                     IDSCVKLGVDTIVTFTGSAFGMNFYGLPGVVPGHPTNHVAENLQIFRDVYGPMSEYAG
                     ERGVKIAFETAGRGGGEGNIAHSPELWDAMFEAVPSKALGLSFDPSHLVWLHIPNIPD
                     IVREFGDRIYHVDGKDTDILPTKLARQGILGSGWWRYRLPGLGAVDWQALFAALKDVG
                     YDGVIAIENEDPLCLGTPGVKWAADYLRAHLLPTPLPAPDPI"
     gene            1862..2155
                     /locus_tag="VFQ54_04710"
     CDS             1862..2155
                     /locus_tag="VFQ54_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164323.1"
                     /translation="MNTEEPSKTTAETGTESQPASEQSTHNKIEVRVDPETGKTVRYY
                     HGVRQIPRDESGPLVTLEMVNKIRDEMDFEDYMEEERSIAERFLDDDAMMRDA"
     gene            2221..2445
                     /locus_tag="VFQ54_04715"
     CDS             2221..2445
                     /locus_tag="VFQ54_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164324.1"
                     /translation="MRTTVNLDEDVLLYVKSVADRSHKTLGETISDLVREAIATNLPE
                     DEDDEMVIPRRPDEPPVTLEFVKSLREELG"
     gene            complement(2957..>3560)
                     /locus_tag="VFQ54_04720"
     CDS             complement(2957..>3560)
                     /locus_tag="VFQ54_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164325.1"
                     /translation="LEPDLIICMGEDDEWFPARKLQRFAPVVPTTYQAYWKDDLKQVA
                     GWLQRTSDLDAGMVAYDKLISDGVAHYGDLIASKKVVYLQYLPDTTNFALNTTGMLQA
                     QILTDLGGTLFGGLLGNSDGDFISLERIGEFAEADGFFVQDLSGSGALDALAELPLWT
                     SLPAVKAGHVVASRGNVNYGSIYTAQELARNWTALYATMA"
BASE COUNT          711 a         1115 c         1060 g          674 t
ORIGIN      
        1 gtcgcgaccg cggtatcctc gacgtcgatg tacgctccgt gacggagcgt cgcgaacttg
       61 ccgtagacct cgaccgcctt gcccatgaac cactggaaat gatcgatcga atggacagcc
      121 tggttcatca ggatgccgcc gccctcggtc gcccacttgc cgcgccacgg atcggcgccg
      181 aagtactccg gaccacgcca gaggcggaca tcgcagaggc cgagcgtgat attgccgagc
      241 ttgccatcgt cgatcgccgc acgaatgcgc tgcgccgccg gccagaagcg gcgctggaag
      301 atcacgccga acttgatccc ggccttgtct gctgccgcga tcatccggtc cgcctcgtcg
      361 atcttggtcg cgattggctt ctcgcacatc acgtggatgc ccgcgttcgc cgccgcgacg
      421 acgacggcct cgtgggccgg gtgcggcgta cagcagagcg ccgcctcgac cagaccgctc
      481 gcgaagagtt ccgccgcgtc gctgaagacg ttcgggacgt tgtatttggc ggccatttcc
      541 ttcgcgcgtt cgacatcgcg atcgcagacg gcgacccaat ccgcttcctc gagcgtgctc
      601 agcgccatcg cgtgagtctg ggcgatcttg ccgcagccaa cgagtgcaat ctggaccttc
      661 cgatcagaca tcctgacacc tcaccgtatt catcgacctg aacgacgttt cgacgcgacc
      721 gtgacttcgt cttctagatg ggatccggcg ccggcagtgg cgtcggcaac aggtgagcgc
      781 gcaggtagtc ggcggcccac ttcacgcctg gcgtcccgag gcagagcgga tcctcgttct
      841 cgatcgcgat cacgccgtcg taaccgacgt ccttcagagc cgcgaagagc gcctgccagt
      901 cgacggcacc gagaccggga aggcgatagc gccaccagcc gctgccgaga atcccctggc
      961 gggcgagctt cgtcggcagg atatcggtgt ccttgccgtc gacgtggtag atgcgatcgc
     1021 cgaactcgcg aacgatgtcg ggaatgttcg ggatgtggag ccagacgagg tgggagggat
     1081 cgaagctgag cccgagcgcc ttcgatggca cggcctcgaa catcgcgtcc cagagctcgg
     1141 ggctgtgggc gatgttgcct tcaccgccgc cgcgaccggc ggtctcgaag gcgatcttca
     1201 cgccgcgttc accggcgtat tccgacatcg ggccgtagac gtcgcggaag atctggagat
     1261 tctcggcgac gtggttcgtc ggatgtccgg gaacaacacc gggcaaccca tagaagttca
     1321 tgccgaaggc agagccggtg aaggtgacga tcgtgtcgac gccgagcttg acgcaggaat
     1381 cgatcgcgag cctgaactcg gcaatccgcg cctcgcgctt cgcgagatcc gccgtcagga
     1441 gattgaccat aggcgccagc gaggcaatct cgaccccgtt ctccgcaagg aggtcggtga
     1501 gttcaccggg tccttcactg gcgatcttcg cgagatcgag gtgaccgcca tacggcgcac
     1561 cagctatcgg cctgtagccg gagccaacgt tgacctcgat cgcatcgaga ccgttggcgc
     1621 ccgcccagcg cacgagttcg ggaaacggca tcggctcgac accgacgagt gacgcgaaac
     1681 ctatcttcat gttgccatcc atcaggttcg atagaaggag accgggagcg atatatcggt
     1741 tgtgtttctg gtggtgcatt gtcctataac ccgggagaaa cgcaaccccc tgcccgacat
     1801 tgattcccga ccgcgatgtg cgacgatgat ggcgtggcct caatatagaa aggcgctgcc
     1861 agtgaacact gaagagccca gcaagaccac cgccgagacc gggacggaaa gccagcctgc
     1921 atcggaacag tcaacccata acaaaattga agttcgggtg gacccagaaa cggggaagac
     1981 cgtccgctat taccacgggg ttcgtcagat tccgcgcgat gaaagcggac cgctagtcac
     2041 cctggagatg gtcaacaaga tccgagatga gatggatttt gaggactaca tggaggagga
     2101 gcgcagcatt gcggagcgct ttcttgacga tgacgcgatg atgcgtgatg cttgattcac
     2161 atgtgcgtat tctcggaacc ttggctttcc caactattgg caaccacgag aggatcggcc
     2221 gtgagaacca ccgtgaatct ggatgaggac gtcttgctct atgtgaagag tgtggccgac
     2281 cgatcccaca agacgcttgg cgaaacgatc tctgacctcg tccgcgaagc gatcgcgaca
     2341 aatctcccgg aagacgagga cgacgaaatg gtgatcccgc gtcgtcccga tgaaccaccg
     2401 gtaacgcttg aattcgtcaa atctcttcgc gaggagttgg gatgaactcc ttcttgctcg
     2461 atgtgagcgt ccttatcgcg atcgtcgacg cgcagcacac cgaacacgtg cgcgcgaagg
     2521 agtggtttga ctccgatcct gagcttcact ggctcagctg acccatcacg cagaacggaa
     2581 cgatcagaat tctcagcaat tccacatatt cgaagaaaat catgccggaa cgcgtgacgc
     2641 gaagtcttca tatcctcacg tcacgcggaa accatctatt cgttcccgat tcgatcagcc
     2701 tgcttgatga cgcgctcatt gactcttccc gccttctcag ccacgcccag ctcacggaca
     2761 cctacctcct ggctctcgcc gcatatcatg gagcgtgctt ggcgacgttt gaccagaagg
     2821 tcgtcacgac agccgtgcga tcggaccata aggaagtttt ccatattcca taggaggcgc
     2881 ccgcgcatca aggcaaggcg ccggtctcct gattgagata ccggcgtctt gccttcacgc
     2941 gttcggatta ctctcgctac gccatggtcg cgtaaagcgc cgtccagttc cgcgccagct
     3001 cctgcgcggt gtagatgctc ccgtagttca cattgccccg actcgcgacg acgtggccgg
     3061 ctttcaccgc cgggagactc gtccagagcg ggagctcggc gagggcgtcc agcgccccgc
     3121 taccgctgag gtcctgcacg aagaacccgt ccgcctccgc gaattccccg atccgctcga
     3181 ggctgatgaa atcaccgtcg ctgttcccga ggaggccacc gaagagcgtt ccaccgagat
     3241 cggtcaggat ctgcgcttgc agcatgcccg tcgtgttgag cgcgaagttc gtcgtatcgg
     3301 ggaggtactg caggtacacc accttcttcg acgcgatcag gtctccatag tgcgccacgc
     3361 catcggagat gagcttgtcg tacgccacca tcccggcatc aagatcgctc gtgcgctgca
     3421 gccaaccggc tacctgcttc aagtcatctt tccaatacgc ctgataggtg gtcgggacga
     3481 ccggcgcgaa tcgttgaagt ttccgggccg ggaaccattc gtcgtcctcg cccatgcaga
     3541 tgatgaggtc cggctcgagc
//
LOCUS       DASTMD010000323         5210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_475942, whole genome shotgun sequence.
ACCESSION   DASTMD010000323 DASTMD010000000
VERSION     DASTMD010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475942"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VFQ54_04725"
     CDS             <1..1446
                     /locus_tag="VFQ54_04725"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164326.1"
                     /translation="HRSLRPLPGRRGVIPACIALLLGMSTLFLMLPSPAAARDILDPV
                     SPTSTAFSSQPTNVLSSDDAGTLYRLVLEARSFGIPLSIRIQSVPSQQEQSSTQSAAQ
                     TLYTDQPVESSSGADDGILLLVQIPSNDKTLSTAAYAVGKNFYPQGGITPDVLDQTIN
                     QYMYPRFKTSTIGSSVINGVTWLVYDQLFLTTPRIPKTHGENSLARITAWVMTPILVI
                     LGILFIGSGFLALRRTRRYRPETPPAPINSPFVAGAIGRGRVDDAVITAGMLALIDRG
                     SLREVRQPARPSTWRVSDDVDPVDPFLQRLWQRAREVAQPETRMIDDATLGQFRDLAT
                     DARKWQEGYLATNGYFHPRARLHNLWLLVAGILLLVIAAYALIPSFIARERAGIFGAI
                     ALTVMVVGTVWWSRGRSFTTAFGAEALDAWTNQRTAATYQGDPTGTSDLETLAVIVDQ
                     QKLIRDGRWLRQRYGDGARQAMGTVLGMGPS"
     gene            complement(1538..2152)
                     /locus_tag="VFQ54_04730"
     CDS             complement(1538..2152)
                     /locus_tag="VFQ54_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU0164327.1"
                     /translation="MSEPITDFSQFAATTLDAVEAVLVTAPGSPSTIDGVIVHGLKTN
                     RDPRGTLTELLRSDWPDIFGETMPFAQVYTSTTAPGVARDIDKWHVHQHQTDRFYCVA
                     GRIVVAIADPRPESPTRGKVMLVELAAQADAPAPMVVTIPPQTLHGFVVTSPGPATLL
                     NFPNRLYDPVDEGRLPFEEAGVTFADGAGFSYDAVRAWYDKNQG"
     gene            complement(2161..3201)
                     /gene="rfbB"
                     /locus_tag="VFQ54_04735"
     CDS             complement(2161..3201)
                     /gene="rfbB"
                     /locus_tag="VFQ54_04735"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU0164328.1"
                     /translation="MKILVTGGAGFIGSTFVRTVIDQHPDDDVITLDALTYAGNLANL
                     AELEGHPRHRFEKGNIADAALIDSLVQEVDAVVNFAAETHVDRSLLYPESFLRSNVHG
                     VYVLLDAVRRHDRRLLHVSTDEVYGDIPEGLFSREGDPVNPRSPYSAAKAGGELFCRS
                     FFVSFGTDVLVTRGCNTIGPRQYPEKLVPLFVTNALRDQPLPVYGDGLQIRDWLHAED
                     HASAIDLALRNGRAGETYNIGAGNERPNIDVATGILAHLGKPDSLITHVPDRAGHDRR
                     YALSFEKIRTELGWEPARTFEQTLNDTIDWYVANTEWWQAIRDNSAEFASYYEKQYAW
                     RLAAATSSERGA"
     gene            complement(3213..4130)
                     /gene="rfbD"
                     /locus_tag="VFQ54_04740"
     CDS             complement(3213..4130)
                     /gene="rfbD"
                     /locus_tag="VFQ54_04740"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU0164329.1"
                     /translation="MTATRERVLVTGAGGQLGRYLRASLASSGYQVVGLGSTLAPGVD
                     VVADITDRDLLASTFGDVAPLAVIHGAAYTDVDGCERDPEKADRVNHLGSRYLAELAA
                     DAGIWMVAVGTDFVYSGNGGAPYSERAVPDPISVYGASKLAGEVAVLATEPSFAVART
                     SWVYGGQGKHFPRTVLSMVANRGGMEVVDDEISAPTFAGDLADALVKLLPHRASGIFH
                     LSNEGSVSRFGLARSVVAAAGGDPAVIAPTTTEAFLAKYPLPAKRPANSTLANNRAAA
                     IGIRLPAWEDAVARYVPRLAAELQAASAT"
     gene            complement(4130..>5210)
                     /locus_tag="VFQ54_04745"
     CDS             complement(4130..>5210)
                     /locus_tag="VFQ54_04745"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEU0164330.1"
                     /translation="TRRSLKGLVLAGGKGSRLRPLTATGAKQLVPVANKPVLFYALEQ
                     MVAAGIEEIGIVIGDTGAQVQAATGDGSAFGARFTYIQQDAPRGLAHALSIGQEFLGD
                     SAFCMYLGDNFLKEGISGHADRFATSNAAGQILLKKVLDVSALGVALLDEDGRVMRLV
                     EKPKTPISDMAVVGVYFFGPEVHQITPNLKPSARGELEITDAIQGLIDAGYEVDSSVI
                     DDEWIDTGKKDDMLEANRVVLSTIGRSVKGDVDALSAIVGDVIIEAGATIIDSVVRGP
                     AVIGSGARIEHTFVGPFTAIGDRCHLLNCEIEHSIVLQDSIIRNIGTRISDSLIGKEV
                     IVERTEQKPHVMRLLLGDHAQVGTI"
BASE COUNT          937 a         1741 c         1593 g          939 t
ORIGIN      
        1 catcgctcgc ttcgtccact tccgggtcgc cgcggagtta tcccggcatg catcgcgctg
       61 cttctcggga tgagtacgct cttcctgatg cttcccagcc cagcggcggc aagggacatt
      121 ctggaccccg tgagtccgac atcgacggcg ttctcgtctc aaccgacgaa cgtcctttcg
      181 agcgacgacg ccggaaccct ctaccggctg gtgctcgagg cccgcagctt cggcatcccg
      241 ctctcgatcc gaatccagtc ggtcccgtcc cagcaggagc agtcgtccac ccagtcggcg
      301 gcgcagacgc tctacaccga tcaacccgtc gagtcgtctt cgggagccga tgacggcatt
      361 ctgctcctcg tgcagattcc gagcaatgac aagacgctct ccacggctgc ctatgccgtc
      421 ggcaagaact tctaccccca gggcggaatc actccggacg tccttgacca gacgatcaac
      481 cagtacatgt acccgcgctt caagacgagc accatcggca gctccgtgat caacggcgtc
      541 acctggctcg tctacgacca gctcttcctc accacaccgc gaataccgaa gacgcatggc
      601 gagaattcgc tcgcacgaat cacagcctgg gtgatgacgc cgatcctggt catccttggc
      661 attctcttca tcgggtccgg attcctcgct ctccggcgca cccgaaggta tcgacccgaa
      721 acgcccccgg ctccgatcaa ctccccgttc gtcgccggcg ccatcggccg cggacgagtc
      781 gacgacgccg tgatcaccgc gggaatgctc gcattgatcg atcgtggttc cctccgggaa
      841 gtacgtcagc cggccagacc atcgacctgg cgcgtgagtg atgatgtaga tccggtcgat
      901 cccttcctcc agcgtctctg gcaacgcgcc cgcgaggttg cccagccgga gacgcgaatg
      961 atcgacgacg ccaccctcgg ccagttccgc gacctcgcca ccgacgcccg caagtggcag
     1021 gagggatacc tcgccaccaa cggttacttc catccgcggg cgcgtctcca caatctctgg
     1081 ctcctcgtcg ccggcatcct gctcctggta atcgcggcct atgccctgat cccgtcgttc
     1141 atcgcccgcg agcgagccgg catcttcggc gcgatcgcgt tgacagtcat ggtcgtgggg
     1201 acggtctggt ggtcgcgcgg tcgctcgttc acgacggcat tcggcgccga ggcgctcgat
     1261 gcctggacga accagcgcac cgcggccacc tatcaaggcg acccgacggg aacgagcgat
     1321 ctcgagaccc ttgccgtcat cgtcgatcaa cagaagctca ttcgtgacgg tcgctggcta
     1381 cgccaacgct atggagacgg cgcgcggcag gcgatgggga cggtcctcgg aatgggtccc
     1441 tcctgagtct tctcgcggca cgtcgagcct cgagaaaaat gaatcggccc ggatcggcga
     1501 atgccgatcc gggccgttcg tgttcagact gtcgagatta tccctggttc ttgtcgtacc
     1561 aggcccgcac ggcgtcgtag ctgaaccctg cgccatcggc aaacgtcacg ccggcttcct
     1621 cgaacggaag acgaccctcg tccacgggat cgtagaggcg attcgggaag ttgaggagcg
     1681 tcgccgggcc cgggctcgtc acgacgaatc cgtggagcgt ctggggcgga atcgtcacca
     1741 ccatcggagc gggcgcatcc gcctgcgccg cgagctcgac cagcatcacc tttcctcgtg
     1801 tcggggattc cggtcgcgga tcggcaatcg ccacgacaat ccgaccggcg acgcagtaga
     1861 agcgatcggt ctggtgctga tgcacgtgcc acttgtcgat atcgcgcgca acgcccggtg
     1921 cggtggtcga ggtgtagacc tgcgcgaagg gcatcgtctc gccgaagata tccggccagt
     1981 cggaacggag aagctccgtc agcgttcccc ggggatcgcg attcgtcttg aggccatgca
     2041 cgatcacgcc gtcgatcgtg gaaggcgacc ccggcgcggt gacgagcacc gcctcgacgg
     2101 catccagcgt cgtcgcggca aattgcgaga aatcggtgat tggctcactc atgagtctga
     2161 ctatgctccc cgttcagatg atgtcgctgc ggcgagtcgc cacgcgtact gcttctcgta
     2221 ataggacgcg aactccgcgg aattgtcacg aatcgcctgc caccattcgg tattggccac
     2281 gtaccagtcg atcgtgtcgt tgagtgtctg ctcgaaggtg cgggcaggtt cccatccgag
     2341 ttcggtgcga atcttctcga aactgagtgc gtaccggcga tcgtgaccgg cccggtcggg
     2401 cacgtgcgtg atcagcgagt ccggtttgcc gagatgggcc agaatccccg tcgcgacatc
     2461 gatattcgga cgctcgttcc cggcgccgat gttgtaggtc tctcccgccc tgccgttgcg
     2521 aagcgcgagg tcgatcgccg acgcgtgatc ctcggcgtgc agccagtccc ggatctgcag
     2581 gccatcgccg tagaccggca atggttggtc ccgcagtgcg ttggtcacga agagcggcac
     2641 cagcttctcg ggatactgcc gggggccgat cgtattgcag ccccgcgtca cgaggacgtc
     2701 ggtcccgaag gagacgaaga acgaccggca aaagagttcg ccgccggcct tcgccgcgga
     2761 gtacggcgat cgcgggttga cgggatcgcc ctcgcgggag aaaagcccct ccgggatatc
     2821 gccgtagacc tcgtcggtgc tcacgtgaag gaggcgacga tcgtgccgcc gaaccgcgtc
     2881 gagcaggacg tagaccccat ggacattcga ccgcaggaag gactcgggat agagcagcga
     2941 tcggtcgacg tgcgtctccg ccgcgaaatt gacgaccgca tcgacctctt gcacgagcga
     3001 atcgatgagc gcggcgtcgg cgatgttgcc cttctcgaac cggtgacgag gatgcccctc
     3061 cagttcggca aggttcgcca agtttccggc atacgtcagt gcatcgagcg tgatcacgtc
     3121 atcgtcggga tgctggtcga tcacggtgcg cacgaacgtc gacccgataa acccggcgcc
     3181 accggtcaca agtatcttca tccattcctc cgtcaggtcg ctgaagccgc ctgcagttct
     3241 gccgccaatc gcggaacgta tcgcgcaacc gcatcctccc acgccggcag gcgaatgccg
     3301 atcgccgcgg cgcggttatt ggcgagtgta ctgttcgcgg ggcgcttggc gggcagcggg
     3361 tatttcgcca ggaaggcttc ggtggtggtc ggcgcaatca ccgccggatc gccacccgcg
     3421 gccgcgacca cggatctcgc cagtccgaac cggctcacgc ttccctcgtt cgagagatgg
     3481 aaaatgcccg acgcacgatg aggaagcagc ttcacgaggg catccgcaag atcgcctgca
     3541 aacgtcggtg cgctgatctc gtcgtcgacg acctccatgc caccccggtt tgcaaccatg
     3601 gagagaacgg tgcggggaaa atgctttccc tgtccgccgt agacccatga ggttcgcgcg
     3661 acggcgaagg acggctcggt cgcgaggacc gcgacttcgc ccgcgagctt gctcgcgccg
     3721 tacaccgaga tcggatcggg caccgcccgc tcgctgtagg gcgcgccgcc attgcccgaa
     3781 tagacgaagt ccgtcccgac ggcaaccatc cagattcccg catcagcggc gagttccgcg
     3841 aggtatcgcg atccgagatg attgacgcga tccgccttct cgggatcccg ctcacatcca
     3901 tcgacgtcgg tgtaggccgc gccgtggatg accgccagtg gcgcgacatc gccgaaggta
     3961 ctcgcgagca gatcgcgatc ggtgatatcc gcaacgacat cgacgccggg agccaacgta
     4021 ctgccgaggc cgacgacctg gtatccggac gatgccagcg atgcccggag gtaccggcca
     4081 agctgaccgc ccgcacctgt caccagcacg cgttcgcgtg ttgccgtcat cagattgtcc
     4141 ccacctgggc gtgatcgccg agcagaagcc gcatcacatg cggcttctgc tcggtgcgct
     4201 cgacgatgac ttccttcccg atcagcgaat cgctgatgcg agtgccgata ttgcggatga
     4261 tgctgtcctg cagcacgatc gaatgctcga tctcgcaatt gagcaggtgg cagcgatccc
     4321 cgatcgcggt gaagggtccg acgaacgtgt gctcgatccg cgcgccgctt ccgatcacgg
     4381 cggggccccg aaccacgctg tcgatgatcg ttgccccggc ttcgatgatc acgtcgccca
     4441 cgatcgccga caacgcatcg acatcaccct tgacgctccg gccgatcgtg ctgaggacga
     4501 cccgattcgc ctcgagcatg tcgtccttct tgccggtatc gatccactca tcgtcgatca
     4561 ccgaggagtc gacctcatac ccggcgtcga tcagtccctg gatcgcgtcc gtgatctcga
     4621 gttcgccccg ggccgaaggc ttcaggtttg gcgtgatctg gtgcacctcg gggccgaaga
     4681 aatagacacc gaccacggcc atgtccgaga tcggtgtctt cggcttttcg acgaggcgca
     4741 tcacacgacc atcctcgtcg aggagcgcga cgccgagagc ggagacgtcg agaaccttct
     4801 tgaggagaat ctgcccggcg gcgttgctgg tggcgaatcg atccgcgtgt ccagagattc
     4861 cctccttcag gaagttgtcg cccagataca tgcagaaggc ggaatcgccg agaaattcct
     4921 ggccgatcga aagggcatgc gcgaggccgc gcggtgcatc ctgctgaatg taggtgaaac
     4981 gcgctccgaa ggcggaacca tcgcccgttg ccgcctgcac ctgggcgccg gtatcaccga
     5041 tgacgatacc gatctcctcg atacccgccg caaccatctg ttcgagcgcg tagaagagga
     5101 ccggcttgtt cgcgaccggc acgagttgct tcgcaccggt ggcggtcagc ggcctcaacc
     5161 gcgacccctt gccgccggcg aggaccaacc ccttcagcga ccgccttgtc
//
LOCUS       DASTMD010000324         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1816526, whole genome shotgun sequence.
ACCESSION   DASTMD010000324 DASTMD010000000
VERSION     DASTMD010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816526"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFQ54_04750"
     CDS             complement(<1..213)
                     /locus_tag="VFQ54_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0164331.1"
                     /translation="MSESRRIAVIGGGISGLAAAHRLVASGDGDSVILLEASNRLGGK
                     ILTEHVDGFTIEGGPDAFLANKPRGVG"
     gene            complement(210..1187)
                     /gene="hemH"
                     /locus_tag="VFQ54_04755"
     CDS             complement(210..1187)
                     /gene="hemH"
                     /locus_tag="VFQ54_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU0164332.1"
                     /translation="MTSESKITNHVMIMAYGGPDKLEDVKPYLLDVRNYRPTSDHIID
                     EITDRYRQIGGRSPILELTREEGAGIEAELNRLAAPGEAWKVWVGMRHWQPFIKDVLA
                     EMEAAGVTDTIGLVMAPHYSRMSIGAYFKRIEEAGSSINVVPIEEWHLLPGYLDGLVD
                     RIGDALLKFPEDVRGSVPIIFSAHSLPERINEWNDPYPHVLQETVAALVERLPGQPHT
                     WAYQSAAMTPDPWLGPDAGEVIERLHAEGHRDILICPIGFICEHVEILYDIDIEYQTL
                     AKKLGVHLERIEMLNANPSMLNGLAGLVRERAIAAGLVGSEATAGTSAS"
     gene            1983..>2223
                     /locus_tag="VFQ54_04760"
     CDS             1983..>2223
                     /locus_tag="VFQ54_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164333.1"
                     /translation="MSHDDLTLNRRRLAGLATVAAVGSILPRSASAQDAAGTPAATPA
                     SGIKTGDVIPPEFVNAAETDWLTENRTMAQDRSVKG"
BASE COUNT          396 a          728 c          687 g          412 t
ORIGIN      
        1 gccgacaccg cggggcttgt tggcgagaaa cgcgtccggg ccaccctcga tcgtgaagcc
       61 atccacatgc tcagtcagga tcttcccgcc gagccggttg ctggcctcga gaagaatgac
      121 ggagtccccg tcgcccgatg caacgagccg atgcgcggcg gcgagaccgc tgatcccgcc
      181 gccgatgacg gcgatccgac gcgactcgct cacgatgcgc tcgttcccgc cgtcgcctcg
      241 cttccaacga gaccggccgc gatcgcccgc tcgcggacga gtccggcgag gccgttcagc
      301 atcgaaggat tggcgttcag catctcgatc cgttcgagat gaaccccgag cttcttcgcc
      361 agcgtctggt actcgatgtc gatgtcatag aggatctcga cgtgctcgca gatgaagccg
      421 atcgggcaga tcaggatgtc tcgatgcccc tcggcatgca gtcgctcgat gacctcgccg
      481 gcgtccgggc cgagccacgg atcgggtgtc atcgccgcgc tctggtaggc ccaggtgtgg
      541 ggctgaccag gcagacgctc gaccagtgcc gcgaccgtct cctggagtac gtgcggatag
      601 ggatcgttcc attcgttgat ccgctcaggc aggctgtgcg ccgagaagat gatcggcacg
      661 cttccgcgaa cgtcctcggg aaacttcagg agcgcatcgc cgatccggtc gaccaggccg
      721 tcgagatagc ccggcaggag atgccactcc tcgatcggaa cgacattgat ggaagatccg
      781 gcttcctcga tgcgcttgaa ataggcgccg atgctcatgc gcgagtagtg gggcgccatc
      841 acgaggccga tcgtatcggt cacgccggcc gcttccatct cggcgagcac atccttgatg
      901 aacggctgcc agtggcgcat ccccacccag accttccacg cctcgcctgg cgccgcgagc
      961 cgattgagtt ctgcctcgat cccggcgccc tcctcccgtg tcagctcgag gatcggggaa
     1021 cgcccgccga tctggcgata gcgatcggtg atctcgtcga tgatgtgatc ggatgtcggg
     1081 cgatagttgc gtacgtcgag caaatagggc ttcacatctt cgagtttgtc ggggccgccg
     1141 taggccatga tcatcacatg attggtgatc ttcgattcgg acgtcataga actcccctga
     1201 tgctgagaat tgggtggtgg gtgcggcgcg ggcccgatct gacgagggat cgggtggctg
     1261 ccgtatgtcg tgctatgggt agcatatcgg ccgcggcaag tgtgtcggga aatacacgcg
     1321 ttcgcaattc cggtacggcg acgctgccac gtctcgccgc gagcaatcgc gatgcgcccg
     1381 ctcggggtaa gatgatcgcg taacgcgccg ccaggggcac ccgcgcagtg ccgtgccgcc
     1441 cgatcctgag gagagaaccg acgtggagcc aatcgaccag cgcaacggaa acatgctcgg
     1501 caaggcggac ctgactgcga tcgccgtggt cgccgtcggc atcatcatcg tgaccacgat
     1561 cctgttgctg ctgctcttct agtcctctcg tcgctccccg acgcgaccgg tccccacgga
     1621 ctccccagga aatcgcgcct catgcgcgac cccgatccgt cactctcctg actcgccact
     1681 tacctgatag ccgcccgtcg tccgcgatcg aggatgaaac ggcgcggaac cacggtcgac
     1741 cccgcttccg gcgttggcca tgcatgcgct gacgggtgaa cccttccctc caagccagag
     1801 acccccgcct cgcagtcggc catacgccga tcgagaagcc atgcacattt atgcgtcgcg
     1861 cgtcacgaat aatctgcgtt cctgcaatta atatagagtc agttcacctc tttcaacatg
     1921 gccgtgcagg gttgcatggc gattcgggcc cgttttgggc ttgagaccag aagggattgc
     1981 tcatgtctca tgacgatctg acgctcaatc gtcgacgatt ggcgggcctg gcgacggtcg
     2041 ccgcagtcgg gagtattctc ccacgctccg cctccgcgca ggatgccgcc ggtacacccg
     2101 ccgccacgcc cgcttcgggg atcaagaccg gagatgtcat tccacccgag ttcgtgaatg
     2161 ccgccgagac cgactggctc accgagaacc ggacgatggc ccaggaccga tcggtcaagg
     2221 gga
//
LOCUS       DASTMD010000325         3526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2261341, whole genome shotgun sequence.
ACCESSION   DASTMD010000325 DASTMD010000000
VERSION     DASTMD010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2261341"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFQ54_04765"
     CDS             <1..331
                     /locus_tag="VFQ54_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164334.1"
                     /translation="FGEDRDRIESLKQRYREGNVGDVEVKGYLAGVLAAVLDPIRERR
                     EKYSATGYVDELIERGTKRVRTETQDTVLAMRRAMGFTGVWNRIHRRAERSQRATEPT
                     HATAERS"
     gene            455..1009
                     /locus_tag="VFQ54_04770"
     CDS             455..1009
                     /locus_tag="VFQ54_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0164335.1"
                     /translation="MRARISKIVLGLILLCGIAVATPSVTIHADEAATPAASATPDGY
                     GGTLVKTTLISDQPASAPGETLALTRFVIPAGSALPVHRHPGIQMATVESGTITYHVI
                     SNGVVLVTRADGTKEQAGPGSTVVLTTGDTWEEPEGMVHYAENLTDQPVVLLSTSLLA
                     TDQDATILVNATPGVATPDATPAP"
     gene            1295..1951
                     /locus_tag="VFQ54_04775"
     CDS             1295..1951
                     /locus_tag="VFQ54_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEU0164336.1"
                     /translation="MAGKKAKSELESIRTEINELTEAIYALRQHVAIESAATAASRQV
                     DGTGRRSGTAVRSTGSETGTIDVSGSIQIARHGDEALAFQWESAGLSIARILDQDHDQ
                     LARVLAAIGHRQRLAILNSILSKPSSAADLVADLQLGTTGAAYHHLNVLQTAGLVKQE
                     ERGIFAVNPERVATIVTILASTSIPTRIGVATSSVDESLDGEEAEPKRKSSKKKATDE
                     "
     gene            complement(2410..2976)
                     /locus_tag="VFQ54_04780"
     CDS             complement(2410..2976)
                     /locus_tag="VFQ54_04780"
                     /inference="COORDINATES: protein motif:HMM:NF015317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HEU0164337.1"
                     /translation="MTDRDEKTQAMIEARERMRDPRHYDRNRDRDAVSGSRFLILLSV
                     LGTTLSAAAMMIFGLIVVAKTIWNTFKDGNFDIEGAKHLAVDLIELTDLFLLGMVLYV
                     VAIGMYQLFINADISIPDWMRVESLNDLKTQLINVIVVLLAVSFLAIAVNWEGATSIV
                     YLGASVCAVIVALAIYNLAHHWVSRDDD"
     gene            complement(3110..>3526)
                     /locus_tag="VFQ54_04785"
     CDS             complement(3110..>3526)
                     /locus_tag="VFQ54_04785"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU0164338.1"
                     /translation="PWLGPIAQNRSGALVASEGGTALAFGLANAQERGITFIEPGTEV
                     YEGMIVGQNPRPTDLAVNICRAKKLTNMRSSNADISVRLTPPTLLSLEQSLDFLQDDE
                     LLEVTPKAYRLRKRLLTQDERAKASKRGKQSVAGVA"
BASE COUNT          691 a         1132 c         1121 g          582 t
ORIGIN      
        1 gttcggcgag gatcgtgata ggatcgaatc gctgaagcaa cgttatcgtg agggcaatgt
       61 tggcgacgtc gaggtgaagg gatacctcgc gggcgttctc gccgccgtcc tggatccgat
      121 ccgggagcga cgcgagaagt actccgcgac cggctacgtc gacgagttga tcgagcgcgg
      181 cacaaagcgg gtacgaacgg agacccagga caccgtcctc gcgatgcgca gggcgatggg
      241 tttcaccggt gtctggaatc gcatccatcg ccgggccgaa cgatcgcagc gcgccacgga
      301 gccaacgcac gcgacagcag agcgatcctg atccgccgcg gccatccgca cgtggggatc
      361 accatcgccg ggaccctacc gttgcggctg agacacgatt tcgaaggatc ggactcgatg
      421 tctcgtcgac gtcactattc acaaggaaat gtccatgcgt gcacggatct cgaagatcgt
      481 tctcggattg atcctgctct gcggaatcgc ggtcgcgacg ccatccgtca cgatccacgc
      541 ggacgaggct gcgacaccag cagcatccgc gacaccagat gggtatggcg gaaccctggt
      601 gaagacgacg ctgatctccg accagccggc gtccgcgccg ggtgagaccc tggcgctgac
      661 ccgcttcgtg atcccggccg gttccgcgct accggtgcat cgccatcctg gtatccagat
      721 ggcgacggtc gaatccggca cgatcaccta ccacgtgatc tcgaacggcg tcgtgctcgt
      781 cacccgcgcc gacggcacga aggaacaggc gggaccaggc tcgacggtgg tcctcacgac
      841 aggagacacc tgggaggagc cggaaggcat ggtgcactac gcggagaacc tgacggatca
      901 gccagtggtc ctgctctcca cgtcgctgct cgcgaccgat caggacgcga cgatcctcgt
      961 caatgcgacc cccggcgtcg ccacgccgga cgcgacgccc gccccctgac ttccggcgag
     1021 acaggcgaga caatcgaaga gacctgaaga tgtcccggca acccaggttg ccgggatatc
     1081 ttcctgttcg aatccaggca gacgcacggc gtgacgccga tccaatctca agagaccgtc
     1141 attggtggga aatacctcgg cgatgggctc cggtccgaca ccctccccac gtacgttctg
     1201 gattgacaaa acgcagacgg gatgctacgt tcctcgataa ctacgtaacg ctgtgcgtgc
     1261 gggaaaggcg agtatccgga gaaggaggaa cgacgtggcg ggcaagaaag caaagtcaga
     1321 gcttgaatcg attcgcaccg agatcaacga actcaccgag gcgatctacg cgttgcgaca
     1381 gcacgtcgct atcgaatccg cggcgacggc ggcctcgcga caggtggacg gcaccggccg
     1441 ccggtccggg accgcggtcc ggtcgacggg cagcgaaacc ggcacgatcg acgtttccgg
     1501 ctcgatccag atcgcgcgtc acggcgatga ggcgctggcc ttccaatggg agtccgctgg
     1561 actttcgatt gcccgcatcc tcgatcagga tcacgatcag ctcgcccgcg tcctcgccgc
     1621 gatcgggcat cgccagcgcc tggcgatcct caactcgatc ctgagcaagc cgagttccgc
     1681 cgcggatctc gtcgccgatc tccagctcgg cacgaccggc gccgcctatc accacctgaa
     1741 cgtgctacag acggcaggac tggtgaagca ggaagaacgc ggcatcttcg cggtgaatcc
     1801 cgagcgggtt gccacgatcg tgacgattct cgccagcact tcgatcccga ccaggatcgg
     1861 cgtggcgaca tcgagcgtgg atgaatccct cgacggggaa gaagcggagc cgaagcggaa
     1921 gtcctcgaag aagaaggcca cggacgagta acacccggac gcttcgtctg gcgcacatca
     1981 cgtcatccca atcgcgaatc acgtcctcgc gggactggct ggtatccgca tgctccccgg
     2041 gcaacgcctc acaacacacg tcctcccgag cctgtcgagg gaccggccgg tgtccgcagg
     2101 ctccccgggc gaggccccac aaaaacgtcc tcccgagctt gtcgagggat cacgcgggca
     2161 ctcccatgct tccagcaaga accttggcaa cgaggctggt tggggagcct cacgcatggg
     2221 acgtggtccc gggcgaacac caggttcgcc cctacggaag cgcgcctgac ggcgtcgcgt
     2281 ccgcgctccg atcgggatga catgttggtg aggacgatgt cgcaggacat gcgtcgccaa
     2341 cgaccagggg cgaataccag gttggcccct acaggatcgc gatcggacgg tgacaggcgc
     2401 cgggcgcggt cagtcgtcgt cgcgagacac ccagtggtgg gcgaggttgt agatcgccag
     2461 ggcaacgatc accgcgcaga ccgaggcgcc aaggtagacg atcgaggtcg cgccctccca
     2521 gttgacggca atcgcgagaa acgagaccgc gagcaggacg acgatcacgt tgatcagttg
     2581 cgtcttcagg tcgttcaggc tctcgacccg catccagtcc ggaatcgaga tgtccgcgtt
     2641 gatgaagagc tggtacatcc cgatcgcgac gacgtagagc accatcccca gcaggaagag
     2701 atccgtcagt tcgatcaggt cgaccgccag atgcttggcg ccctcgatgt cgaaattccc
     2761 gtccttgaag gtgttccaga tcgtcttcgc cacgacgatc agcccgaaga tcatcatcgc
     2821 cgccgcgctc agggtcgttc ccaggaccga gaggaggatc aggaatcgcg agccactgac
     2881 cgcatcccga tcgcgattgc ggtcgtaatg acgaggatcg cgcatccgct ccctggcttc
     2941 gatcatcgcc tgtgtcttct cgtcccgatc tgtcatcacg tcccccaacg ttcgcgctcg
     3001 ttgccaccca accaggtcga tcgtatgcga ggaggacgtg gatacgcaaa caccggactc
     3061 gtttgagtcc ggtgttcgcg tcaattcgaa tcgggaaacg gaagagctat caggcgacgc
     3121 cggccacgga ctgcttcccg cgcttgctcg ccttcgcgcg ctcgtcctgc gtcagcaggc
     3181 gcttgcggag gcgatacgcc ttcggcgtca cttccagcaa ctcgtcgtcc tggaggaagt
     3241 ccagtgactg ttcgaggctg agaagggtcg gcggcgtcag gcggaccgag atgtccgcgt
     3301 tgctggaccg catgttggtc agcttcttgg cgcggcagat gttgacggcg agatcggtcg
     3361 ggcgcgggtt ctggccgacg atcatgccct cgtagacctc ggtgcccggc tcgatgaagg
     3421 tgatgccgcg ctcttgcgcg ttcgcgagac cgaaggccag cgcggtgccg ccttccgagg
     3481 caacgagcgc gccgctgcga ttctgggcga tcggcccgag ccatgg
//
LOCUS       DASTMD010000326         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1279864, whole genome shotgun sequence.
ACCESSION   DASTMD010000326 DASTMD010000000
VERSION     DASTMD010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279864"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VFQ54_04790"
     CDS             complement(<1..728)
                     /locus_tag="VFQ54_04790"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEU0164339.1"
                     /translation="MTDASPPLSSQSTVDRFLIRLIVFAGGMTSVGTETSAARLLAPF
                     FGESTLIWANIIGFTLTFLALGYWIGGRVADRYPRPWLLFTATAVAAIWAALTPLISR
                     PLLGRSVTAFEDLSVGAFYGSLIGILLILAVPITLLGFVSPYAIRLSVPDVAHAGEAA
                     GGIYALGTVGSIAGSFLPTLLLIPWVGTVRSFLILGLLLLVPSVIGFLRLREVPLAVG
                     SVTLGIAMVIASSFYRPASIRPSQG"
     gene            985..1470
                     /locus_tag="VFQ54_04795"
     CDS             985..1470
                     /locus_tag="VFQ54_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0164340.1"
                     /translation="MGQDSTTVAEASVSDLVDRMQTVIPQFQHFARHVLSSIQGDDRV
                     TLQQLRSLQAIAGTDGGMSTTKLAKRLRIASPTVTRIVDGLVDRGMVDRVQDPNDRRR
                     LRLLLLAPGQDLLDQYEQALHARLAVRLHDLPPPSRQRLWDALEDLNLVLDDPDRDAT
                     D"
     gene            1499..>2031
                     /locus_tag="VFQ54_04800"
     CDS             1499..>2031
                     /locus_tag="VFQ54_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0164341.1"
                     /translation="MVTSPVATERPPSVVPVIAPSRMTSERPAIEVNGLVRKFEDFTA
                     VNGISFAVPHGEVFGFLGPNGAGKSTTIKMLCTLLRPTGGSAYVNGYNVQEDPSGVRN
                     SIGVIFQDFSLDDRITAEENLRFHCMIYHVPRSERKERIARVLEMVNLTDRAGTKVKT
                     FSGGMKRRLEIARGLLHR"
BASE COUNT          423 a          654 c          638 g          316 t
ORIGIN      
        1 ccctgggacg ggcggatcga cgccggccgg tagaacgaac tcgcgatcac cattgcgata
       61 ccgagcgtga ccgatccgac tgccagcggg acctctcgca gccgaagaaa gccgatcacc
      121 gagggcacga gcaggagcaa gccgaggatc aggaacgagc ggaccgttcc cacccagggg
      181 atgagcagca gtgtcggcag gaagctcccg gcgatcgacc cgaccgtgcc gagcgcatag
      241 attccaccgg ccgcttcacc ggcgtgggcg acatccggaa cgctgagccg gatcgcatac
      301 ggtgagacga agccgagcag ggtgatcggc accgcgagaa tgagcagaat cccgatcagc
      361 gatccataga aagcgccgac cgaaagatcc tcgaaggcgg tcaccgagcg gccgagaaga
      421 gggcgggaaa tcagcggcgt caaggccgcc cagatcgcgg cgaccgccgt cgccgtgaag
      481 agcagccacg gacgtgggta gcggtcagcg acgcgaccac cgatccagta tccgagggcg
      541 aggaatgtca gggtgaagcc gatgatgttc gcccagatca gcgtcgactc gccgaagaac
      601 ggcgcgagca gacgcgcggc gctcgtctcg gtgccgacgc tcgtcatgcc gccggcgaag
      661 acgatcagcc ggatcaggaa gcgatcgacg gtggattggg aagatagggg aggggatgca
      721 tcagtcaagt gttctgcctg ttccgggctc gacgatcgac aacgattgcc aaggacgatg
      781 ggaaggacgc tcgaagatca gcgtgaatcc agtataggga ctccaatcac ggcgatatcc
      841 cggcatctct cgccttccgg cgacctgtag ccttgacatt gattagtatt gctacataat
      901 agatggacta ccgatgaacg ccgtcgaccg cgccatctgg cacgaggaca gacgcattca
      961 tcgccacagg caggggacgt gcacatgggc caggactcaa caacggttgc cgaagcatcg
     1021 gtcagtgatc tcgtcgatcg catgcagacc gtgattccgc agtttcagca cttcgcccgg
     1081 cacgttctct cctcgatcca gggtgatgac cgggtgacgc tccagcagct gcggagcctc
     1141 caggcgatcg cgggcaccga cggcgggatg tcgacgacga agctcgcgaa gcggctgcgg
     1201 atcgcctcgc cgacggtcac ccggatcgtc gacggcctcg tcgatcgcgg catggtggat
     1261 cgcgtccagg atccgaacga tcgccgccgc ctgcgactgc tccttctcgc gccgggtcag
     1321 gacctcctcg atcaatacga gcaggcgctt catgcgcggc tggcggtgcg attgcacgat
     1381 ctcccaccgc ccagccgcca gcgcctctgg gatgccctgg aggacctgaa cctcgtcctc
     1441 gacgatcccg atcgcgacgc cacggattga ccctgccact caacggaaag ctcacgaaat
     1501 ggttacatca cccgtcgcta ccgaacgtcc acccagcgtg gttcccgtca tcgctccctc
     1561 ccgaatgaca tcggagcgcc cggcgatcga ggtcaacggt ctcgtccgca agttcgagga
     1621 tttcaccgcg gtcaacggaa tctcgttcgc ggtgccgcac ggcgaggttt tcggctttct
     1681 cggcccaaac ggcgctggca agagcacgac gatcaagatg ctgtgcacgc tgcttcgtcc
     1741 gaccggcgga tccgcctacg tcaacgggta caacgtgcag gaggatccct ccggcgtccg
     1801 caactcgatc ggcgtcatct tccaggactt cagtctcgac gaccggatca cggccgagga
     1861 aaatctccgc ttccactgca tgatctatca cgttccgcga tcggaacgaa aagagcggat
     1921 cgcccgcgtg ctcgagatgg tgaacctcac ggatcgcgcc ggcacgaagg tcaagacctt
     1981 ctccggtggc atgaagcgcc gcctcgagat cgcgcgcggc ctgctccacc g
//
LOCUS       DASTMD010000327         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_476067, whole genome shotgun sequence.
ACCESSION   DASTMD010000327 DASTMD010000000
VERSION     DASTMD010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476067"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VFQ54_04805"
     CDS             <1..976
                     /locus_tag="VFQ54_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164342.1"
                     /translation="FPDYGPNNYRQGSLSYQLVSAGESVTLTFANTENAGPYIDDVST
                     SCTNPTPTETNTPVSTRTDTPTAVPSDTPNPTGTGTTLPTGPGPVAPTPTSTTAPPTS
                     TVIPSPDTGAPNSTATIVPSVTVAPSSTSVPTGTAETTGSVTLILTTSDGGPVPNGAI
                     VCVGDQCQAVGASVSSAAVGTTLTFVGLDPGTYPLTVTNAAPYLDATGSVTVTAESTT
                     NASIVLQLAAVTVTTVPTEPGAATPIVTGTAGPTQAPGDGNAIPTQRAGGSDGAAPPS
                     RTDGAPAVKALPNTGAGGGSSDASLTLLLLAIVAVAGGGILTWRRRRI"
     gene            complement(1099..>2300)
                     /locus_tag="VFQ54_04810"
     CDS             complement(1099..>2300)
                     /locus_tag="VFQ54_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0164343.1"
                     /translation="GPNGVAVAYGMVYAATGDTSVVFALDAKTGKEVWSNDVSNNDFE
                     CIDMAPAVYDNIVYISTNPNNTTNGNYRGGARGIIWALDAATGHTLWSFDTSTGNLWG
                     NPRTNSGAGLWYPPTFDEAGNMYVGTGNSAPYPGNKEYPNASSRSEPNDYAASMVSIS
                     LDTGAVNWSVNALPHDLYDHDFQNSPILAHVPLNGTDFLVAMGSGKTGTVIGADAKTG
                     AMLWKTNVGKHMNDQLEELPPGTTEIWPGSSGVQTPGAFADGKYFAAYRDKPEYLTPT
                     GADATKSYAMTDETGGLAAIDAATGTIVWDVKIPTVMVVGAAVANDLVFTGGTDGILR
                     AFDVKTGKAVWQFQAGSGMNAPLAIAGDTIFFAATAPFVLHTPAGGTPVPSPAPKFAV
                     YALKLSQ"
BASE COUNT          368 a          772 c          757 g          403 t
ORIGIN      
        1 ctttccggac tatgggccga acaactaccg gcaaggctcg ctgagctatc agctcgtcag
       61 tgccggtgag tccgtcacgc tcacgttcgc caacacagaa aacgccggcc cgtacatcga
      121 tgacgtgagt acatcctgca ctaaccccac gccgaccgag acgaacacac cggtgtcaac
      181 gcgaaccgat accccgacgg cggttccctc ggacacgcca aatccgaccg ggaccggcac
      241 gactctccca accggcccgg ggccagtcgc ccccacgccg acatcgacca ccgctccgcc
      301 cacgtcgacc gttatcccct cgccggatac aggtgctccg aactccaccg cgacgatcgt
      361 cccctccgtc accgttgcac cgagctcgac cagcgtcccg accggcaccg ctgagacgac
      421 cgggtccgtg acgttgatcc tgaccaccag tgatggtggg cctgtcccga acggggcgat
      481 cgtctgcgtt ggtgaccagt gccaggcggt gggagccagc gtcagctcgg ccgccgtcgg
      541 gacgaccctg acgttcgttg gtctcgatcc cggcacctat cccctgacgg tgaccaatgc
      601 tgcgccgtat ctggacgcaa ccggcagcgt gacggtcacg gccgagagca cgacgaacgc
      661 gtcgatcgtg ctgcagctgg ctgccgtcac tgtcacgacg gtcccgacgg aacccggggc
      721 ggcaaccccg atcgtgaccg ggaccgcggg cccgacgcag gctccaggtg acggcaatgc
      781 gatccccacc cagcgggcgg gaggcagcga tggcgcagcc cccccgtccc gcacggatgg
      841 agcgcccgcg gtgaaggcgt tgccgaacac cggtgccggc ggcggatcga gcgatgcgag
      901 cctgacgctg ttgctgctgg cgatcgtggc ggtggccgga ggagggatcc tcacctggcg
      961 tcggcggcga atctagcggc cggaacgccc tgagagacga tcgacttccc gaaatcatgg
     1021 tggggagtgg gtgtcgacac gccacacgcg ccgggagaca ttcctcccgg cgcgtgtggt
     1081 gctgttgttg cgtatcggtt actggctgag cttcagcgca tagaccgcga acttcggtgc
     1141 cggtgacggg acaggcgtcc cgcctgccgg cgtgtgcaga acgaacggcg ccgtcgcggc
     1201 gaagaagatc gtgtccccgg cgatcgccag cggcgcgttc atgccggatc ccgcctggaa
     1261 ctgccagacg gccttgccgg tcttgacgtc gaaggcgcgc agaatgccgt ccgtgccgcc
     1321 ggtgaagacc aggtcattgg ccaccgcggc gccgacgacc atcacggtcg ggatcttgac
     1381 gtcccacacg atcgtgccgg tggcggcatc gatcgcggcg aggcccccgg tctcatcggt
     1441 catcgcgtac gacttggtgg catctgctcc ggtcggtgtc aggtactccg gtttgtcgcg
     1501 gtaggcagcg aagtacttgc cgtcggcgaa cgcgcccggc gtctggacgc cggagctgcc
     1561 cggccagatc tccgtggtgc ccggcggcag ttcctcgagc tggtcgttca tgtgcttgcc
     1621 gacgttggtc ttccagagca tcgccccggt cttggcgtcc gcgccgatta ccgttcccgt
     1681 cttcccggat cccatcgcca cgaggaagtc cgtcccgttc agcggaacgt gggcgagaat
     1741 cggcgaattc tggaagtcgt gatcgtagag atcgtgcggc agcgcgttga ccgaccagtt
     1801 gaccgcaccg gtgtcgaggc tgatcgagac catgctcgcc gcgtagtcat tgggttcgga
     1861 tcggctgctg gcgttcgggt attccttgtt gccgggatac ggcgccgaat tgccggtccc
     1921 gacatacatg ttgcctgctt cgtcgaacgt cggcgggtac cagaggccgg cgccggaatt
     1981 cgtgcgcgga ttcccccaca ggttgcccgt cgaggtatcg aagctccaga gcgtatgccc
     2041 ggtcgcggca tcgagagccc agatgatgcc gcgcgcgcct ccgcggtagt ttccgttcgt
     2101 ggtgttgttc ggattggtgc tgatgtagac gatgttgtcg tagaccgccg gcgccatgtc
     2161 gatgcactcg aagtcgttgt tggagacgtc gttgctccag acctccttgc ccgtcttggc
     2221 atcgagcgcg aagacgacgc tggtgtcgcc ggtcgcggcg tagaccatcc cgtaggccac
     2281 cgcgactccg ttcgggccat
//
LOCUS       DASTMD010000328         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1904431, whole genome shotgun sequence.
ACCESSION   DASTMD010000328 DASTMD010000000
VERSION     DASTMD010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904431"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFQ54_04815"
     CDS             <1..668
                     /locus_tag="VFQ54_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0164344.1"
                     /translation="VTLKQEGLTSVAQNEDQRWTVATDSGAPEETFDAVIATVPSNIF
                     QKMVPGLPEAYQKKLDALEYEAAVVAILEVDRPLSNIYWLNIADDDLPFTGIIEHTNF
                     IDPATYKGRRFIYLSKYMEPDHPYFSMDSDSIIKEYLPFLKRVNPNFDPSWVKNSWVF
                     RERAAQPIIPLNYSEMIPDHRTPLKNLYLANTTQIYPEDRGTNYSVRLGNDIAKLVLG
                     DLA"
     gene            complement(676..>1509)
                     /locus_tag="VFQ54_04820"
     CDS             complement(676..>1509)
                     /locus_tag="VFQ54_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0164345.1"
                     /translation="IIRADERRVILIDTGLNDTTARKVLRVVRDELGSDVAAILTTHG
                     HADHFGANAFVIKRTTARVYAPDLDEIVLRFPLMQSILLYGGADPVDSLRTKFLLAEA
                     GPVDQVVKAGTIDVEGVEIEAVSLKGHSINQLGYLIDGVFFCADVVFPQAALEKYPIP
                     YLYGLTEHLDSMERALDIECTVVVPGHGPVEARMSEPVARNRVVISRVLEAIPELLRE
                     PLTADALADSLFTALDVPVNDASGYFLLRPTVSAYLSHLARTGVISSFVAGKSIVWQR
                     R"
BASE COUNT          315 a          480 c          453 g          261 t
ORIGIN      
        1 gcgtcacgct gaagcaggag ggtctgacct ccgtcgccca gaacgaggat cagcggtgga
       61 cggtcgcgac cgatagcggc gctcccgagg agaccttcga cgccgtgatc gcgacggtgc
      121 cgtcgaacat cttccagaag atggtgccgg ggctccccga ggcctaccag aagaagctcg
      181 acgccctcga atacgaggcg gcggtcgtcg cgattctcga agtcgaccga ccgctgagca
      241 acatctactg gctcaacatc gcggacgacg atctgccatt cacgggcatc atcgagcaca
      301 ccaacttcat cgatcccgcg acctacaagg gccgtcgttt catctacctc agcaagtaca
      361 tggaaccgga ccatccctat ttctcgatgg attccgactc gatcatcaag gaataccttc
      421 cgttcctgaa gcgggtgaat ccgaacttcg atccgagctg ggtcaagaac tcgtgggtct
      481 tccgcgaacg cgccgcgcag ccgatcatcc cgctgaacta ctccgaaatg atccccgacc
      541 accgcacccc gttgaagaat ctctacctgg cgaatacgac gcagatctac cccgaggatc
      601 gcggcaccaa ctacagtgtc cgcctcggca acgacatcgc gaagctcgtc ctcggcgatc
      661 tcgcgtagtt cgccgtcagc ggcgctgcca gacgatggac tttccggcga cgaagctgga
      721 gatcacacca gttcgcgcga ggtggctgag gtaggcggaa accgtcggac gcagcaggaa
      781 gtagccggag gcgtcattca ccgggacgtc gagagctgtg aagagggaat ccgcgagcgc
      841 gtccgcggtc aacggctccc gcagcaattc gggaatcgcc tcgagcaccc ggcttatgac
      901 aacacgattc cgggcgactg gctcggacat ccgggcctca accggaccgt gtcccggcac
      961 gacgacggtg cattcgatgt cgagcgcacg ctccatcgaa tcgagatgct ccgtcaaccc
     1021 gtagagatat gggatggggt atttctcgag cgccgcctgt gggaagacca cgtccgcaca
     1081 gaagaagacg ccgtcgatca ggtagccaag ctgattgatc gagtgcccct tgagcgacac
     1141 cgcctcgatc tccacccctt ccacatcgat cgtcccggcc ttgacgacct gatcgacggg
     1201 accggcttcg gccagcagga actttgtccg caacgaatcg acgggatcgg cgcctccgta
     1261 gaggaggatc gactgcatca gcggaaaccg aaggacgatt tcgtcgaggt ccggagcata
     1321 gaccctggcg gtggttcgct tgatcacgaa cgcgttggcg ccgaagtggt ccgcgtggcc
     1381 gtgcgtggta aggatcgcgg cgacatctga accgagctcg tctcgcacga cgcggaggac
     1441 ttttcgcgcc gtcgtgtcat tcagcccggt gtcgatgagg atcacccggc gctcatcggc
     1501 gcggatgat
//
LOCUS       DASTMD010000329         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1012371, whole genome shotgun sequence.
ACCESSION   DASTMD010000329 DASTMD010000000
VERSION     DASTMD010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012371"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2008
                     /locus_tag="VFQ54_04825"
     CDS             <1..2008
                     /locus_tag="VFQ54_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164346.1"
                     /translation="VGNGTAPAATATTTPAPTATATATSAPTTAPTTAPTSAPTSPSG
                     GDSGIGGLLNGLVNTVAGQGGIVDSVAGTGGVLDDVLPGDGGLADVDLQTNADVSVLG
                     TNDQGTPSVLDANADICLQLAILGSTGPGCAPVSNATNDDDQLVNADTDACVGVAILG
                     STGGCDLSGGNGTPEATPGSNDAVDGDVDACVGIAILGSTGTCSTNTGSTDTNGDGIA
                     DVDGDACANIAILGSTGNCSTSTGTDSGGNGVGDVDADACAGIAILGSTGNCSTSAGT
                     DPTGDSTAGVDADACANIAILGSAGDCTTSTGTGTSGDGLVDGDVDACAGIAILGNTG
                     DCQTTESGGGDGLVNVGGDVCANVAILGSTDGCVESQAVTPTVTATATATATATSTPG
                     GTVTPTSTAIATQTPVGTETPVGTQTPTGTQTPAETETPVGTETPVGTQTPTGTQTPT
                     DNETPTSTQTPMGTQTPTGTETPVGTQTPISTQTPTGNETPTSTQTPTGTQTPTGNEI
                     PTATPGGPGGPSDSGNPGGPSDPGNPIPSIPSGAGGNAGSSSNLGNSALSPASPTPVS
                     AVNNLPDAGSGPGSNLQGSSANPDIILAVALALMALAGAIALRRRPDQLVNDLFVPST
                     ASTSTAAPMGIPQERGGTPKLTRRSTSYRVSPRRRAPRSRRTYS"
BASE COUNT          444 a          814 c          692 g          316 t
ORIGIN      
        1 cgtcggcaat ggcacggctc ccgccgcgac cgcgacgact accccggctc cgacagccac
       61 ggcaaccgcg acttccgctc ccacgaccgc cccgaccacc gctccgacgt cggctcccac
      121 gagcccgtcc ggaggcgata gcggcattgg cgggctgctc aatgggctcg tcaacaccgt
      181 cgccggtcag ggcggcatcg tcgattccgt cgccggtacc ggtggcgttc tcgatgacgt
      241 cctccccggc gacggggggc tcgccgatgt cgatctgcaa accaatgcgg acgtcagcgt
      301 gctcggcacg aacgaccagg gcaccccgtc ggtgctcgat gcgaacgccg acatctgcct
      361 ccagctcgcg atcctcggca gcaccggacc gggctgcgcg ccggtctcca acgccacgaa
      421 tgacgacgat cagctcgtca acgccgacac cgatgcttgc gtcggcgtcg caattctcgg
      481 cagcaccggc ggctgcgatc tctcaggcgg caatggcacg cccgaggcaa cgccgggcag
      541 caacgacgcc gtcgacggtg atgtcgacgc ctgcgtcggg atcgcgatcc tcggctccac
      601 gggcacctgc tcgacgaata ccggcagcac cgacaccaat ggtgacggca tcgccgatgt
      661 cgatggcgat gcctgtgcga acatcgcgat cctcggctcc acggggaact gctcgaccag
      721 cactggtacg gatagcggtg gaaacggtgt cggcgacgtc gatgccgacg cctgcgccgg
      781 tatcgcgatc ctcggcagca ccgggaattg ctccacgagt gccggaaccg atccgaccgg
      841 ggatagcacc gcgggcgtcg atgcggacgc ctgcgccaac atcgcgatcc tcggcagcgc
      901 tggagattgc acgacgagca ccggcaccgg aacaagcggc gacggactgg tcgacggcga
      961 tgtcgacgcc tgcgcgggca tcgcgatcct cggcaacacg ggtgattgcc agacgaccga
     1021 aagtggcggc ggcgatggtc tcgtcaacgt cggtggtgac gtctgcgcca acgtggcgat
     1081 cctcggcagt accgatggat gcgtcgagag ccaggcggtg acaccgacag ttaccgccac
     1141 ggcgactgcg accgcaaccg caacctcgac tccgggcggc acggttacgc cgacgtccac
     1201 cgctatcgca acgcagactc cagtcggaac cgagactcca gttggaacgc aaaccccgac
     1261 cggcacgcag actccagccg aaaccgagac tccggtcgga accgagactc cagttggaac
     1321 gcaaactccg acgggaaccc agacgccgac tgacaacgaa acgccaacca gcacccagac
     1381 tccgatggga acgcagaccc caactgggac cgagactccg gtcggaacgc agacgccaat
     1441 cagcactcag actccgacgg gcaacgagac gccaaccagc actcagactc cgacgggaac
     1501 ccagacgcca accggcaatg agatcccgac agctactcct ggtggacccg gcggaccgag
     1561 cgattccggc aatcccggcg gaccgagtga tcccggcaat ccgatcccat cgatcccaag
     1621 cggagccggc ggcaatgctg gttcgagctc caaccttggc aactcggcgc tcagtcccgc
     1681 ttcacccaca cctgtgagcg ccgtcaacaa ccttccggac gccggttccg gcccggggag
     1741 caatctccag ggcagctcgg ccaaccctga catcatcctc gcggtcgcgc tcgcactgat
     1801 ggccctggcc ggggcaatag cccttcgtcg gcggcccgat cagctcgtga atgacctgtt
     1861 cgttccttcc acggcttcga cgtccaccgc cgctccgatg gggattcccc aggagcgcgg
     1921 cggaacaccg aagctgacac gacggagcac cagctatcgc gtttcaccgc gacggcgcgc
     1981 tccgcgctca cgacgaacct attcctgatc cgggtcaccg gacgcttcgc ggatgcggat
     2041 cacgctgctg tgatccgcat ccggagggcc ggcgccggat tcccggcgat ctgccccact
     2101 ggaattcgtt ggacgtgacg cgcggaacgc aattgcagag ttgtctgatc tcaccgaacc
     2161 acgctgccgg ctatgacagc ctcccggtag cgcggttcat gggaccagcc gaacgcaggt
     2221 aacacgagcg tccatgtcga tgtgaaagga ccgaaccaac ctccac
//
LOCUS       DASTMD010000330         1596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2976132, whole genome shotgun sequence.
ACCESSION   DASTMD010000330 DASTMD010000000
VERSION     DASTMD010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2976132"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFQ54_04830"
     CDS             complement(<1..838)
                     /locus_tag="VFQ54_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164347.1"
                     /translation="MDRRKLSGLLLVSLLFSLFARVGPVAAAPVATPDDGVTGVKSVT
                     AKVLCYGSSKFSKQMIVWVNNNSSRPIEVVYVGGIDYGAPQNPASVVSGWNQAVEQSV
                     APGETITYETNLGNDSDKVFGGALVVTSRGILQPMCNEQVVHLWSDPGLPANNSEDAM
                     NQEVTIAVTTIGQLESLRAYPALYAMMHPDSQAHLTVDTLSCWYAKQFGVPSDWKETV
                     FTTTIDKLDIVNSWVWAPERIRYERGVAIVDITQSIGGIAKTQDVTSTLHLVESAGVW
                     RWF"
     gene            complement(1226..>1596)
                     /locus_tag="VFQ54_04835"
     CDS             complement(1226..>1596)
                     /locus_tag="VFQ54_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164348.1"
                     /translation="RNQAGTAADRDKVIAPAENLMNFAYQGAFDLATAPVSPNGVAIQ
                     TELVENFLALSDLYAIDMLGAFAGLYGLDQSGIDQLTSQFNQKSDLTIQQHDQIAQDW
                     NDLAGQCGVGAEFPALPGSK"
BASE COUNT          301 a          485 c          500 g          310 t
ORIGIN      
        1 agaaccagcg ccacactccg gccgactcga cgagatggag tgtcgacgtc acgtcctgcg
       61 tcttcgcaat gccaccaatg ctctgggtaa tgtcgacgat ggcgacaccc cgctcgtagc
      121 gaatcctctc cggcgcccag acccaggagt tgacgatgtc gagcttatcg atcgtcgtgg
      181 tgaagacggt ctccttccag tcactcggca cgccgaactg cttcgcgtac cagcatgaga
      241 gcgtgtcgac cgtgagatgc gcctgcgaat cgggatgcat catcgcgtaa agcgccggat
      301 acgcccggag cgactccagc tgtccgatcg tcgtcaccgc gatcgtgacc tcctggttca
      361 tcgcatcctc cgagttgttc gccgggaggc ctgggtcact ccagagatgc acaacctgct
      421 cgttgcacat cggctgcagg atgcccctgc tcgtgacgac cagagctcca ccaaagacct
      481 tgtcggaatc gttcccgaga ttcgtctcgt aggtaatcgt ctctcccggc gcgactgatt
      541 gctccaccgc ctgattccag cccgagacga ctgaagctgg attctgcggg gcgccatagt
      601 cgatgccgcc aacgtagacg acctcgatcg ggcggctcga attgttgttg acccagacga
      661 tcatctgctt gctgaatttc gaggacccgt aacagagcac ctttgcggtg accgatttca
      721 cgccggtcac gccatcgtcc ggagtggcga ccggcgccgc cgcaacggga ccgacacgtg
      781 caaaaagcga gaacagcagt gagacaagaa gaagacctga cagcttgcgt cgatccatga
      841 ctgactccta tgacgtgttc gcgattgtcc agaatgggag gaggcgcttc gcagggagtg
      901 cgaaatccgg aaccgtcgac acgtttggtg gcaccggggg ggttggtagg gcggggtttc
      961 ataccccgcc gcaagacgga ctcgggaaca ggacgtcgtg cccgaggtgg ctaccgttgc
     1021 gcgtttcgtt cccgtcccgg caccccacgt ggtcgccctg tgtcaatgcg gcgcagccga
     1081 tccgcttccg gagcgcgtcg tgcgtgggag agtccgaaga tgtccggctg cgccggatgt
     1141 gcttcgcacg gggatttctc gacaggctcg aaatgacgaa actttatgcg acagcgttgg
     1201 attttatatc ccgccgcaaa gctgactact tcgagcccgg gagggccgga aactcggcgc
     1261 cgacaccaca ctggccagcg aggtcattcc agtcctgcgc gatctggtca tgctgctgga
     1321 tcgtgaggtc ggacttctgg ttgaactgcg acgtgagttg atcgatcccg gattgatcca
     1381 ggccgtagag acctgcgaag gcaccgagca tgtcgatcgc gtagaggtcg gagagggcga
     1441 ggaagttctc gacgagttcg gtctggatcg ccacaccgtt cggcgagacg ggcgcggtcg
     1501 cgaggtcgaa cgccccctgg taggcgaagt tcatcaggtt ctcggcgggc gcgatcacct
     1561 tgtcccggtc cgccgcggta ccagcctggt tgcgct
//
LOCUS       DASTMD010000331         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_743320, whole genome shotgun sequence.
ACCESSION   DASTMD010000331 DASTMD010000000
VERSION     DASTMD010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743320"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VFQ54_04840"
     CDS             complement(<1..950)
                     /locus_tag="VFQ54_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164349.1"
                     /translation="MTAATSPESIEGDPLVEAATRAGLRHTSLDRPGITRERSTTDWC
                     FRNPDGTIVRDERVLDRIRAIVIPPAWRDVWINPDPRGHIQATGLDSRGRKQYRYHPQ
                     WRAYRDETKFAHLAAFGAALPGIRDQVACDLARHGFPREKVLATIVRLLDTSLIRIGS
                     REYERTNGSFGLTTLRNRHVAVVGSTIHFTFKGKGGIEHTVDLQDRRLAAIVKRMREL
                     PGYHVFQYVDGDHRRHDVSSEDVNGYLREISGEQFTAKDFRTWAGTVRFVADVRDEPT
                     PPSATEASRRVVAAIRCVSAALGNTPAVCRASYIHPAVIEA"
     gene            complement(1024..2274)
                     /locus_tag="VFQ54_04845"
     CDS             complement(1024..2274)
                     /locus_tag="VFQ54_04845"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164350.1"
                     /translation="MPEIALRHNRDFRLLWIGQAVSTLGSNISAVSYPLLVLATGGSA
                     AAAGLIGFLAMLPNAVVQLPAGALVDRWDRKRVMLVSDAGRLIALATVAIAVIAGSPP
                     LALLAVAALIEGTLSVFFNLAEFGAVRHVVPETQFSDAIAQNEARTRGAALLGQPLGG
                     FLFGLRHAVPFLADAISYLISFTTLAAIRADFNDERTATSNLRRLPSEIREGVGWIWR
                     QPFLRAAALIFAANNFIVQALVLSLIVLTREHGAPAGEIGLMLAGFGVGGLIGAIASP
                     WLQSRISPRAVIAGSNWLCAIAIPLMALWPHPAPIAALAGAMALLGPMVNVVMGTARL
                     RLTPDALLGRVTSASGVIAAGAIPFGSLIGGALLARFGGQHTILILAGLTVILAIVAT
                     WSPDVRRYPAPEATRTASRQFTPE"
     gene            complement(2410..>3017)
                     /locus_tag="VFQ54_04850"
     CDS             complement(2410..>3017)
                     /locus_tag="VFQ54_04850"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0164351.1"
                     /translation="GPTDADAATQPAALSADAPRRQRSDAKRNVVALLEAAKSVFART
                     GVDAPPKEITDLAGVGVGTLYRHFPRRSDLIVAVLQHEVDACASAATTLSAEHGPWEA
                     LRMWVGRFTEFVGTKRGLATALHSGDPAFDDLPRRLMADLEPALDELLSAAKASGDVR
                     VDVTASDILLTVALMCQPVPGEDLRFSERMIRIFIEGLRHP"
BASE COUNT          503 a          981 c         1070 g          463 t
ORIGIN      
        1 gcctcgatga cggccggatg gatgtaggaa gcccggcaga ccgccggcgt gttgcccagt
       61 gcggcggata cacatctgat cgccgcgacc acccggcggc tcgcttccgt cgccgatggt
      121 ggcgtcggct cgtcgcggac atcggcgacg aagcggacgg tgccagccca ggtccggaaa
      181 tccttcgccg taaactgctc gccgctgatc tcgcggagat agccgttgac atcctcggag
      241 gagacatcgt ggcggcgatg atctccgtcg acatactgga agacgtggta gccgggaagc
      301 tcccgcattc gcttgacgat tgccgccagc cgccggtcct ggagatcgac cgtgtgctcg
      361 atgccgccct tccccttgaa ggtgaaatgg atcgtcgatc caacgacggc gacatggcgg
      421 ttacggagcg tcgtcaggcc gaacgacccg ttcgtgcgct cgtactcgcg actgccgatc
      481 ctgatcagcg aggtatcgag gagcctgacg atcgtggcga gcaccttttc cctcgggaag
      541 ccgtgcctgg caaggtcgca cgcgacctgg tcgcgaatcc ccggcagcgc ggccccgaac
      601 gccgcgaggt gggcgaactt cgtctcgtcc cggtacgcgc gccattgggg atggtagcgg
      661 tactgcttgc gcccgcgact gtccaatccg gtcgcctgga tgtgcccacg cggatcgggg
      721 ttgatccaga catcgcgcca cgcgggcggg atgacgatcg cgcgaatccg gtccagcaca
      781 cgctcgtcgc gaacgatcgt cccatccgga ttccggaagc accaatccgt ggtagatcgt
      841 tcgcgggtga tcccgggacg gtcgagcgac gtatggcgca gtccggcccg cgtcgccgct
      901 tccaccaggg gatcgccctc gatcgactcc ggggatgtgg ccgcggtcat cctctgcctc
      961 gctggtttgc atgtcgatcc gatccgtgat ccatgcgggc gcaggcgcac ggatcgtgcc
     1021 aattcactcc ggggtgaatt gccgggatgc cgtcctcgtt gcctcgggcg cagggtagcg
     1081 acgaacgtcg ggactccagg tcgcgacgat tgcgaggatg acggtcaggc cggcgaggat
     1141 caggatcgta tgctggcctc cgaagcgggc gaggagggcg ccgccgatca gggaaccgaa
     1201 cggaatcgcg ccggccgcga tcacgccgct ggcgctggtc acgcggccga gcagcgcgtc
     1261 cggcgtcagg cggaggcggg ccgtccccat cacgacgttg accatcggcc cgagcagcgc
     1321 catcgctccc gccagcgccg cgatcggggc gggatgtggc cagagcgcca tcagtggaat
     1381 cgcgatcgcg cagagccagt tcgatcccgc gatcaccgcc cgtggcgaga tccgggattg
     1441 cagccagggc gaggcgatcg cgccgatcag ccccccgacg ccgaatcccg ccagcatcag
     1501 cccgatctcg ccagccggtg cgccatgctc gcgcgtgagc acgatcaggc tgaggaccag
     1561 cgcctggacg atgaagttgt tcgccgcgaa gatcagcgcc gccgcgcgga gaaagggttg
     1621 ccgccagatc cagccgaccc cttcgcggat ctcggatggc agccgccgga gattcgaggt
     1681 cgcggtgcgc tcgtcgttga aatcagcgcg aatcgcggcg agcgtcgtga acgagatcag
     1741 ataggaaatg gcgtcggcga gaaacggcac ggcgtgtcgc aggccgaaga ggaagccgcc
     1801 gagcggctga ccgagcaggg ccgcgccgcg cgtgcgggcc tcgttctggg cgatcgcgtc
     1861 ggaaaactgg gtctcgggga cgacgtgacg gactgccccg aactccgcga ggttgaagaa
     1921 gacggagagc gtcccctcga tcagggccgc cacggcgaga agcgcgagtg gtggcgatcc
     1981 cgcgatcacc gcgatcgcga cggtggcgag cgcgatcagg cggccggcgt cgctgacgag
     2041 catcacgcgc ttgcggtccc agcggtcgac gagcgcgccg gccggcagct ggacgacggc
     2101 gttcggaagc atcgcgagga agccgatcag accagccgcg gccgccgatc cgcccgtcgc
     2161 caggacgagg agcggatagg aaaccgccga gatattggag ccgagtgtcg agaccgcctg
     2221 cccgatccag agcaggcgaa aatcgcgatt gtgacgcagg gcgatctcag gcatcgacga
     2281 cgacaactcc tcatccggaa ctggtgacac acatgtcgtt ccggggagtg tagggggtga
     2341 tcgcgttccg tgaacatccg gcgccgtctc gtccgcattg ctcaaccctg atcggcttcc
     2401 ggaggaccgt tacgggtggc gtagcccctc gatgaagatg cggatcatgc gttcgctgaa
     2461 gcgcaggtcc tcgcccggga cgggctgaca catgagcgcg acggtgagca ggatgtcgct
     2521 ggcggtaacg tccacccgga cgtcgccgct ggctttcgcc gcgctgagga gttcgtcgag
     2581 ggccggctcg aggtccgcca tgaggcggcg agggaggtcg tcgaacgccg ggtcacccga
     2641 gtgcagtgct gtggcgagtc cgcgcttggt gccgacgaac tccgtgaacc gtccgaccca
     2701 catcctgagg gcttcccacg gcccatgctc cgcgctgagg gtcgtcgctg cgctggcgca
     2761 cgcgtcgacc tcgtgctgca gcacggcgac gatcaggtcg gaacggcggg ggaagtgccg
     2821 gtagagtgtc ccgacgccga caccggcgag gtctgtgatc tccttcggcg gcgcgtccac
     2881 gcccgtgcgg gcgaacactg actttgcggc ctcgagcagg gcgaccacat tgcgtttcgc
     2941 gtcagagcgc tggcggcgcg gcgcgtccgc gctcaacgct gccggttggg tcgcggcgtc
     3001 agcatcggtc ggtccca
//
LOCUS       DASTMD010000332         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1638710, whole genome shotgun sequence.
ACCESSION   DASTMD010000332 DASTMD010000000
VERSION     DASTMD010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638710"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFQ54_04855"
     CDS             <1..249
                     /locus_tag="VFQ54_04855"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEU0164352.1"
                     /translation="DLNRWLPIVKWLLAIPHYIALFFLDILLVIAVIVAWFAILFTGK
                     YPRDLFTFVEGVLRWNNRVVAYALILVTDDYPPFRLSA"
     gene            297..494
                     /locus_tag="VFQ54_04860"
     CDS             297..494
                     /locus_tag="VFQ54_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164353.1"
                     /translation="MWWQDFLWGLWNGATAWFVLIAHAFGAWDRFPLYDVTKTDNWYD
                     FGFLIGAGSPLLGAFRRRGHD"
     gene            complement(591..1574)
                     /locus_tag="VFQ54_04865"
     CDS             complement(591..1574)
                     /locus_tag="VFQ54_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0164354.1"
                     /translation="MSEQTENLEIARLRKQVRDQGFAIDTLQRQVDEVIFAQRLADIA
                     HVDLVKYTGPSKTVEPNPTAQGAGNPVIINAHVYVPKDLDSSRQHPLVLFIHGGVHAR
                     HSSSATNVVRELVEQGYTVVSPDYRGSTGYGQGFQEAIDYGGREVDDSYAAREWALEA
                     YDHLDPARVGIIGWSHGGLITLFNIFDHPEDFAVAYAAVPVSDLIARMGYKSQAYRDL
                     FSAKYHIGKTAEEDVAEYRRRSPSWNTDRYAGTPLLIHTNTNDEDVNVLEVERLIQAL
                     AASGHTVESKIYQDAPGGHSFNRLDTRLARESRREVWRFLAPHLNPQRPVE"
     gene            complement(1815..>2005)
                     /locus_tag="VFQ54_04870"
     CDS             complement(1815..>2005)
                     /locus_tag="VFQ54_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164355.1"
                     /translation="DYAMSPGGPGYEFLVSALLEYVRTSWGSAEGFFKAAGVDDETIA
                     AVRARLIAHQPEAFGNDD"
BASE COUNT          330 a          611 c          652 g          412 t
ORIGIN      
        1 gacctgaaca ggtggttgcc aatcgtcaag tggctgctcg cgattccaca ctacatcgcg
       61 ctgttcttcc tggacatcct gctggtgatc gccgtgatcg tcgcctggtt cgcgatcctc
      121 ttcaccggca agtacccgcg cgaccttttc acattcgtgg agggagtcct ccgctggaac
      181 aatcgcgtgg tcgcctatgc gttgatcctc gtgaccgacg actatccgcc gttcagactt
      241 tcggcgtgat atcgcgggcg tccgaatccg tctgatcgcc gagaagggaa tgcttgatgt
      301 ggtggcagga ttttctgtgg ggactctgga atggcgcgac ggcatggttc gtcctcatcg
      361 cccacgcctt cggcgcgtgg gaccggttcc ccctgtatga tgtgacgaag accgacaact
      421 ggtatgactt cggcttcctg atcggcgccg gatcgcctct cctcggcgcc ttcaggcgtc
      481 gcggtcacga ctgaccctcg tcgcgtggaa cgaacgaaaa ccgccggaac attcgggatg
      541 ttccggcggt tttcgtgact gcggcccggg tcgggatgct tgcgaagaaa ctactcgacg
      601 gggcgctgtg gattgaggtg gggcgcaagg aaccgccaga cctcgcgtcg actttcgcgg
      661 gcgagccgcg tatcgaggcg gttgaaggaa tggccgcccg gggcgtcctg gtagatcttg
      721 ctctcgacgg tgtggccgct tgccgcgagg gcctggatca ggcgttcaac ctcgagcaca
      781 ttcacgtcct cgtcgttcgt gttggtatgg atcagcagtg gcgttcccgc gtaacgatcc
      841 gtgttccatg atggcgatcg ccgtcgatac tcggcgacat cctcctcggc cgtcttgccg
      901 atgtggtact tcgcggagaa gaggtcgcga tacgcctggc tcttgtagcc catgcgcgcg
      961 atgagatcgc tgaccggaac cgccgcgtag gcgaccgcga agtcctccgg gtggtcgaag
     1021 atgttgaaga gggtgatcaa cccgccgtgg ctccagccga tgatgccgac ccgggcggga
     1081 tcgagatgat cgtacgcttc cagcgcccat tcgcgggcgg cgtacgagtc gtcgacttca
     1141 cgaccgccgt agtcgatcgc ttcctggaaa ccctggccgt agccggtgct tccgcggtaa
     1201 tccggtgaga cgacggtgta gccctgctcg acgagttcgc gaaccacgtt cgtggcggac
     1261 gagctgtggc gggcatggac gccgccatgg atgaagagca ccagcggatg ctgacgcgag
     1321 gaatcgaggt ccttgggcac gtatacgtgc gcgttgatga tgaccggatt gccggcaccc
     1381 tgggccgtcg gattcggttc gaccgtcttg ctcggcccgg tgtacttgac cagatcgacg
     1441 tgggcgatat ccgccagccg ctgcgcgaag atcacttcgt caacctggcg ttgcagcgtg
     1501 tcgatcgcga aaccctgatc ccgaacctgc ttccggagac gcgcgatctc cagattctct
     1561 gtctgttcac tcaccgtctg gatctccctg gatgaaccgt taacccgcga cacagtcaat
     1621 cacgagcctc ggcatgtgtc cagagcaccc ggcaagccgt ctcgtccggc cgggatatcc
     1681 gttgtcaaag ggagagcagc ccgtcgtggc tgccgcaaca agaagggacc cggcgactgt
     1741 ggtgccaccg ggtccggttc actcgcttcg ttgttttcgc gctgcgggcg ggcgggttgc
     1801 gtctgaggga gcgatcagtc gtcgttgccg aatgcttccg gctggtgggc gatcaggcgg
     1861 gctcgcaccg cggcgatcgt ctcgtcatcg acgccggcgg ccttgaagaa gccttccgcc
     1921 gaaccccagc tggttcgaac gtactcgagg agggcagaca ccaggaactc gtagccggga
     1981 ccgcccggcg acattgcgta gtccg
//
LOCUS       DASTMD010000333         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_743703, whole genome shotgun sequence.
ACCESSION   DASTMD010000333 DASTMD010000000
VERSION     DASTMD010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743703"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFQ54_04875"
     CDS             <1..722
                     /locus_tag="VFQ54_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0164356.1"
                     /translation="IDAEVLIKIKESITGNNSDYWHYEYNLNQVSGGLWKLDVGYQCA
                     VNKTGTADDYCTAITYPDGSKDVGATASHSTEIHDLAIGNTANNQTTGFFGTHSAPPP
                     SGSKQVVKYAQLYETAFFQQYNVYDHASIRTFDVCVPNKASFSGVTRIRKSELDALTE
                     KVARDSYGRYLKRITLKKIRGFEDRSVRFDFPVTAIVGPNGHGKTTVLGAAALIYKDI
                     KPRRFFAKSGKYDATMRSAVA"
     gene            1357..1992
                     /locus_tag="VFQ54_04880"
     CDS             1357..1992
                     /locus_tag="VFQ54_04880"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU0164357.1"
                     /translation="MEERLQLRQRSPLRTGRDHRRAQVRSGQRRRRFTASMLSVHERP
                     FGPGDRSEPGHWEGDLIVGPAHRSAIGTLVEHQTRVAKLIHLERADSVSLHRALVRDL
                     AGLPPHLLRSITWDQGTEMATHEMIAADLGVKVYFCDPCSPWQRPSNENTNGLLRNYF
                     PRSTDLSVHSREDLRRVEHELNHRPRAILGHRPPTDLFAALLASSQRPPLQ"
BASE COUNT          510 a          778 c          737 g          477 t
ORIGIN      
        1 atatcgacgc agaggtcttg atcaagatca aggaatccat caccggaaat aactctgact
       61 actggcacta cgaatacaat ctgaaccaag tttctggcgg cctctggaaa ctggacgtcg
      121 gctaccaatg tgcggtgaat aagaccggta cagccgatga ttattgcacc gcgattactt
      181 atcctgacgg cagtaaggat gttggtgcca cggcctccca ttcgacggag atccatgatc
      241 ttgcaatcgg gaatactgcc aacaaccaaa ccactgggtt cttcggtaca cattccgcac
      301 ctccaccctc gggctcgaag caggtggtga agtacgccca gctctacgag accgcgttct
      361 tccagcagta caatgtctac gatcacgcga gcattcgtac ctttgacgtg tgtgtaccaa
      421 acaaggcgag cttctccggc gtgacgcgaa tcaggaagag cgaactggat gcgctaaccg
      481 agaaggtagc gcgggacagc tacggacgct atctgaagcg gatcactcta aagaagatcc
      541 ggggatttga agaccgttcg gtacggtttg actttcccgt cacggcgatc gttggaccta
      601 acgggcatgg caagacgacg gtcttgggcg cagcagcctt gatctacaaa gacattaaac
      661 cgaggcgctt cttcgccaag agcggcaagt acgacgccac aatgcggagt gccgtggcct
      721 gaacgccatg aagtcggccc tcgcggagcg ttcggttccc attggtggcg ccagatcgtc
      781 gtgcgggatc cgtcgcgcgc acaagtatac ccgctctgat gaggctggac gcggcgattt
      841 ccctaccaga agcttcgacc gttcgaaggg ctaaacgaac tgtcggcttg gggaagcgct
      901 tctgtgacga acgtgtgcgt acgcgtccga gccaactgac tgccgcgatt cggcactaag
      961 gatcgaatat cgaacgacga agctcctgat cactactcat agcgtggggt caaggcgaac
     1021 cagtgaaggc ccctttggcg aaacagatca attgatgctc agtccgaacc gaatttcagg
     1081 catgggtggt cacaccctcc ctgacgcgga ccccggccgg tcgttgacgg tgtcgacgac
     1141 gcttgaagac gaggccgaag cgacggggtg gtttctagat cactgcaaca cgtgcctgat
     1201 cctggagccc atgttgtcca ctcgtcgcgt tgccaagggc ctcggccgtc gtccgcagtc
     1261 ggctaagcgt gaacagttcg ttgacctgct cgcgaggggg tggagcgtcg ctgcggcccg
     1321 gcgcgaggtc ggcgtcagcc gcacgaccgg tgaccggtgg aagaacggtt acaacttcgc
     1381 caacgctcgc ccttgcggac ggggcgtgat caccgccgtg ctcaggtgcg gtccgggcag
     1441 cgccgacggc gattcaccgc cagcatgctg tccgtccacg agcgcccgtt cgggccgggt
     1501 gatcgcagcg agcccgggca ctgggagggc gaccttatcg tcgggccggc gcaccgctca
     1561 gcgatcggca cgctggtcga gcaccagacg cgcgtggcca agctaatcca cctcgagcgc
     1621 gccgactcgg tatcgctgca tcgagcgttg gtccgcgacc tcgctggtct cccgccgcac
     1681 cttctccgct cgatcacctg ggaccagggc accgagatgg cgacacacga gatgatcgcc
     1741 gcggacctcg gtgtgaaggt ctacttttgc gatccctgct cgccctggca gcgaccaagc
     1801 aacgaaaaca ccaacggcct gctgcgcaac tatttcccga gatccacgga cctctcggtg
     1861 cactcgcgag aggacctacg ccgcgtcgag catgaactca accaccggcc gcgagcgatc
     1921 ctcggccacc gtccaccgac cgacctcttt gctgcgctgt tagcctcttc ccaacgcccg
     1981 cctttgcagt gatcgctaga ggccaccccg tcgcttcggc ctcagattcg agcgtcgccg
     2041 acaggcgatc gactcgtgat ctgggctcaa acatcaagcc ccacgtccgg acgcagccgc
     2101 agagaccaac cgtgtcgaca tacgcagcta ccccgacatc gccggcgcag caccgcaaat
     2161 ccgccaccat cgaccacgta cctaccgtat atagcggtac cggtctgccg cggcagcgat
     2221 ggagaaggcc cgtcgagccg ccgtcgttgt tgttgagcaa tcactgcagc ggatcgatga
     2281 ccgacgcttc ctgttcagat tgtccattcg acagatcaga tcgttgggtg acggcggcac
     2341 cccgggagtc gtcgtcacgt acgacgtgtg ttcgggtttg acgagctgtc ggtatgggat
     2401 ggatcacgcc gagaccgtct cgtggaacgt tccatcactt ggctaaggtt aggcttccct
     2461 aattcggagg cgttgtgtct actgcttctc gtttccagcc gt
//
LOCUS       DASTMD010000334         5544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2530650, whole genome shotgun sequence.
ACCESSION   DASTMD010000334 DASTMD010000000
VERSION     DASTMD010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530650"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFQ54_04885"
     CDS             complement(<1..213)
                     /locus_tag="VFQ54_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164358.1"
                     /translation="MVQRSSATRTSGALPRSIGLGGVQGWLDGLGSSLRRKSPPGMRP
                     SGGDPGPKADLRSRLIVALPRLGIALT"
     gene            complement(555..2420)
                     /locus_tag="VFQ54_04890"
     CDS             complement(555..2420)
                     /locus_tag="VFQ54_04890"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEU0164359.1"
                     /translation="MAQDDPSPARDQPAGSTAGSDDAGIAADDGKAAAWTPAGRGRLS
                     LRSLLRESMPANDIARAGAVRPRLGVTPSRIGVTATSLLLIAVAALFAFRIIYTDRIY
                     PAVVVGDVPVGGLTVPQAQAKVVDRAADFEGGLITFAYGGRSWSPTFTDLGATIDVTQ
                     SVEDAARLGRDGDTTSRLTFAGDLMREDQRVPLRTTIDLAVLDVWFDHVDDEIDRHAV
                     DAGIVIVDGAPSITPDATGIVVDRAAATTQIVASLQRLEPVQLALPTTTEYPAVSAGD
                     LEPFQGQIRAALAKPIAITFEQRRWEVDPADLVEFITADTRVVDGKPVVKLALDVDPL
                     ASWLNQRFAPEVNRSPVDAKLGWSDDDGLVAVEPAQDGATLKPKTFAENLSANFLGGK
                     TIPIPVVTTRPEIDDSNLDALGITTRLGRGDSNFSGGDENRDTNIYIGVELANGTLVP
                     PGADYSFNGAVGPITEDKGYVVSNVIFGETPGVDIGGGICQVSTTVYRAALMGGFPIA
                     ELHPHTYRILNYEYDDWGPGFDASILQLGDDPAAWSDLRFTNDTGGWLLVETWASYPH
                     VVVSIYGRPVDRSVEITGITTGTSEEGGLIAGFTRVVRDGRGEILSEEEYSTTFK"
     gene            3071..3811
                     /locus_tag="VFQ54_04895"
     CDS             3071..3811
                     /locus_tag="VFQ54_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0164360.1"
                     /translation="MAHIVIVEDERELAHLVAQALREDGHITDIINDGQTALDRLTGP
                     GIVAPDLIILDLMLPGIDGLTICRKIRQERIVPIVMLTAKSTELDKVLGLELGADDYL
                     TKPVALRELQARVSAILRRVEMMRVESQTQDPSDEPIAHDGLRIDPLGREVVVNGVEI
                     VLTAKEFDLLYLLASHPGRVFSRNYLLDRLWGDSFSGFERTVDTHIQRLRRKLGREGN
                     AADRIVTLWGVGYKYERAGAPHLAANSQ"
     gene            3808..5463
                     /locus_tag="VFQ54_04900"
     CDS             3808..5463
                     /locus_tag="VFQ54_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0164361.1"
                     /translation="MIDRSRVSPSGRDASSSPAARLWSWVIWPWRLFQQRTSIQMIAS
                     YIVAFMLAIVLLQATIVGSLSWGPAARFFGIDNVTIDPYFGERARSYSQWIGVDDLVD
                     LTSGAASPATQLKISASLDQITAGTIPGFDLSGSVGASVADAAVIAPGGSIVATSDPA
                     WMPVGSTIASFPSKTLQDIAARSLALGGGIDPKTENLYSLTSSDGQTFAATPIIAADG
                     SYRGSLLIEGAPLSDQFGADRLEFLQMVSWEYAKQLWIFSIPALLVAIPFGVWRSRSI
                     SRRLDRLAGAADALAQGNLKTRVVISRHDEIGRLAERFNEMGQQIEDTDRARRAFISN
                     VSHDLRTPVSIIHGTAERMVAMDERGEGVDADSLSVIQQETTTLTRLIDDLFTLARIE
                     EHSLRLERDSFQIAGVVKEAVDGLRDLAWTQQKVTVESLVSRDLPLVFADRTRIRQVI
                     NNLLYNALRHTPEGGLIVVQAVAVNEYVEVSITDTGRGIPANVLPNLFTRYWQAERSK
                     RRSGGSGLGLTIVKQLVEAHQGEITVESQVGQGTTFRFTIPQR"
BASE COUNT         1008 a         1896 c         1677 g          963 t
ORIGIN      
        1 cgtcaacgcg atgccgagac gcggcaaggc gacgatcagg cgcgacctga ggtcggcctt
       61 cggcccggga tctcctccgg aagggcgcat ccccggcggg gacttgcgcc ggagcgaact
      121 ccccaaccca tcgagccaac cctggacgcc gccaagcccg attgaccggg gaagcgctcc
      181 actcgttcgt gtagcagacg atcgctgaac cattgtttct ccagccccag gagctcttgc
      241 gggcgacgcc cacgtctgaa aagcacgaca gacctgcagc gaaacgtgtg gcagatgcct
      301 gtcgggaagg cgacctgtcg ggctgcgggt gagagacgat tcggtggtct tgatctggga
      361 tgtgcgctta ccggcgggcc ggctcccccg cacatgccaa gccattctac gccttcgcgg
      421 ccgttccggc acgtttctgg tgcgaatcgg cgatcaggcg caccccaccc gactctcaat
      481 cctgtgaacg cgcggatttc cgattcggat tcacatgctg gcaatatcca tgttgccggg
      541 cgtcgaaatg cacatcactt gaacgtcgtc gagtactcct cctcggagag gatctcgcct
      601 cgcccgtcac gcacgacgcg ggtgaaaccg gcgatgagac cgccctcctc cgaggttccc
      661 gtcgtgatgc cggtgatctc caccgaacga tcgaccgggc gcccgtagat gctgacgacg
      721 acgtgtggat acgacgccca cgtttccacg agcagccagc ccccggtgtc attcgtaaac
      781 cggaggtcgc tccacgcggc tggatcatcg ccgagctgaa ggatcgacgc gtcaaagccg
      841 ggtccccagt cgtcatactc atagttgagg atccggtagg tatgtggatg aagctccgcg
      901 atcggaaagc ctcccatcag ggcggcgcga tagaccgtgg tcgagacctg acagatgccg
      961 ccgccgatat cgacccccgg cgtctccccg aagatcacgt tcgagacgac gtaccccttg
     1021 tcctccgtga tcggcccgac ggcgccattg aaggagtaat ccgcgcccgg cggcacgagc
     1081 gtgccgttcg ccagctcgac accgatgtag atgttggtgt cgcggttctc gtcgccaccg
     1141 gagaaattgg agtcgccgcg cccgagacgg gtcgtgatcc cgagcgcatc gagattcgag
     1201 tcgtcgatct cgggccgcgt cgtgacgacg gggatcggga tcgtcttgcc gcccaagaag
     1261 ttcgcgctca ggttctccgc gaacgtcttc ggcttcaacg tcgcgccatc ctgggctggt
     1321 tcgaccgcga ccagcccgtc gtcatcgctc cacccgagtt tcgcatcgac cggcgaacga
     1381 ttcacctcgg gggcgaaacg ctggttcagc cacgacgcaa gcggatccac atcgagggca
     1441 agcttgacca ccggcttgcc gtcgacgacg cgggtgtcgg cggtgatgaa ctccacgaga
     1501 tcggcgggat cgacctccca cctccgctgc tcgaacgtga tcgcgatcgg cttcgccagg
     1561 gccgccctga tctgcccctg gaatggctcg agatcgcccg ccgagaccgc cggatactcc
     1621 gtcgtcgtcg ggagcgcgag ttgaaccggt tcgagacgct ggagcgacgc gacgatctgc
     1681 gtcgtcgcgg ccgcgcgatc gacgacgatc cccgtcgcat ctggcgtaat cgagggcgca
     1741 ccatcgacga tcacgatccc ggcatcgacg gcgtgacgat cgatctcgtc atcgacatgg
     1801 tcgaaccaga cgtccaggac cgcgagatcg atcgtcgtgc gcaggggaac gcgctgatcc
     1861 tcccgcatga gatcgccggc gaacgtcagc ctcgatgtcg tatcgccatc ccgcccgagt
     1921 ctcgccgcgt cctcgaccga ttgcgtgacg tcgatcgtgg cgccgaggtc ggtaaaggtc
     1981 ggcgaccagg atctcccacc ataggcgaag gtgatcagac cgccctcgaa gtcggcggct
     2041 cgatccacga cctttgcctg cgcttgcgga acggtcaatc cgccaacagg gacatcgccg
     2101 accactacgg ccggatagat acgatccgta tagatgatcc tgaatgcgaa gagggcagcg
     2161 acggcaatca gcaggagaga ggtcgcggtc accccgatcc gcgagggcgt cacgcccagg
     2221 cgagggcgga cggcgcccgc ccgggcaatg tcgttcgcgg gcatcgattc gcggaggagt
     2281 gacctcaggg agagacgccc acggcccgcc ggagtccacg ccgcggcctt cccgtcatcg
     2341 gcggcgatgc ctgcgtcgtc gctgcctgcg gtcgatcccg cgggctggtc tcttgccgga
     2401 gaaggatcat cctgcgccat acgtaccgtg catcctcgct gttgggatca ggatccggat
     2461 cgttgcgatg agatttcggt gaatgagcga tggttctcat gaggaaacgt ggcaaaggga
     2521 catccggtgt cacctgcaac gacgatttcc tccgaaaatg cgcggacatc gaggagataa
     2581 gcgggacacg ggctgggtta tcgaagacgg gacgtcgccg gattgctttc cctgcgagta
     2641 tgccacgcgg tcacacggcg ggtcacgacc tgccgcaaga taccgcgtga aaccggaccg
     2701 tatagccttt cccaagcttt gccaccaatt cccatgagga tcgtgacagt ttcgtgacac
     2761 acgcgccgca gttcctcagc agtccggacc tatcatgaag gagtcgaaag cgagagagcc
     2821 tctccccccc ggttctaccg gttgcgttcc tcacctagac aatccctccc ccacccccct
     2881 gggacggtcg tatccagcca atcacgaccg tcccaacccc tctcccttcc ccaccaacgc
     2941 atcccgccaa ccgtcccgca cccggtgtac gggcgcgcta cggtcgctca tcctttacca
     3001 ttaaatccac acgcccacgc ggccggttgg ccatatcgtg gaagggaccc gggagctggg
     3061 agagcgacac atggcccaca ttgtcatcgt cgaggacgaa cgcgaactcg cgcatctcgt
     3121 ggcgcaggcg ctccgtgagg acggccacat cacggatatc atcaacgacg gccagaccgc
     3181 gctcgatcgg ctcaccggac ccggtatcgt cgcacccgat ctgatcattc tcgacctcat
     3241 gcttcccggc atcgacggcc tcacgatctg tcgcaagatc cgccaggagc gcatcgtgcc
     3301 gatcgtcatg ctcaccgcga agtcgaccga actcgacaag gtcctcggtc tcgaactcgg
     3361 cgccgatgac tacctgacaa aacccgtcgc cctgcgcgag ttgcaggcgc gcgtctccgc
     3421 gatcctccgg cgcgtcgaga tgatgcgcgt cgagtcccag acgcaggatc cgagcgacga
     3481 accgatcgcc cacgacgggc tccggatcga ccccctcgga cgcgaggttg tcgtcaacgg
     3541 tgtcgagatc gtgctgacgg cgaaggaatt cgatctcctc tacctgctcg cctcgcaccc
     3601 cggtcgcgtc ttcagccgca actacctgct cgaccgcctc tggggagatt ccttctcggg
     3661 attcgagcgc acggtcgaca cgcacatcca gcgcctgcgc cgcaagctcg gtcgtgaggg
     3721 caatgccgcc gaccggatcg tcacgctctg gggcgtcggc tacaagtacg agcgcgcggg
     3781 cgcccctcac ctcgccgcca atagccaatg atcgaccgat cgcgcgtatc gcccagcggg
     3841 agggacgctt ccagttcccc ggcggcgcgt ctctggagct gggtcatctg gccgtggcgt
     3901 ctcttccagc agcgcaccag catccagatg atcgccagct acatcgtggc gttcatgctc
     3961 gcgatcgtcc tgctccaggc cacgatcgtg ggatcgcttt cctggggtcc ggcagcgcgt
     4021 ttcttcggga tcgacaacgt gacgatcgac ccgtacttcg gggagcgcgc ccggtcctac
     4081 tcgcagtgga tcggtgtcga cgacctcgtc gatctcacct ctggcgcggc atcgccggcc
     4141 acgcagctga agatcagcgc atcactcgac cagatcaccg cgggtacgat tcccggcttc
     4201 gatctttcgg gatcggtcgg cgcgagcgtc gcggatgccg cggtgatcgc accgggagga
     4261 tcgatagtcg caacgtcgga tccagcgtgg atgccggtgg gctcgacgat cgcgtcgttc
     4321 ccatcgaaga cgctgcagga tatcgccgcc cgcagcctgg cgctgggtgg aggcatcgat
     4381 cccaagaccg agaatctcta ctcactgacc tcatccgacg ggcagacatt cgccgcgact
     4441 ccgatcatcg ccgccgatgg atcctaccgg ggttcgcttc ttatagaagg cgcccccctt
     4501 tccgatcaat tcggcgccga ccggctggag ttcctccaga tggtctcctg ggaatacgcg
     4561 aagcaactct ggatcttctc gatccccgcc ttgctcgtcg ccatcccctt cggcgtctgg
     4621 cgctcgcggt cgatctcgcg acgtctcgac cgcctcgccg gcgctgccga tgcactcgcc
     4681 cagggaaatc tcaagacccg cgtcgtcatc tcccgccacg acgagatcgg ccgtctcgcc
     4741 gagcgattca acgagatggg ccagcagatc gaggacaccg accgcgcgcg tcgtgccttc
     4801 atctcgaacg tctcccacga tctcaggacg ccggtctcga tcatccacgg caccgcggag
     4861 cggatggtgg cgatggacga gcgcggcgaa ggcgtcgacg ctgactccct cagcgtcatt
     4921 cagcaggaaa cgacgaccct gacccggctg atcgacgatc tcttcaccct cgcccggatc
     4981 gaggagcaca gcctccgtct cgagcgggac agcttccaga tcgccggcgt cgtcaaggaa
     5041 gcggtcgacg ggttgcggga cctcgcctgg acgcagcaga aggtgacggt cgaatcactc
     5101 gtcagccggg acctcccgct cgtgttcgcc gatcgcacgc gcatccgcca ggtcatcaac
     5161 aatctcctct acaacgcgct ccggcatacc ccggaaggcg gcctgatcgt cgttcaggca
     5221 gtcgccgtca acgagtacgt cgaggtctcg atcacggata cgggtcgcgg cattccggct
     5281 aacgtccttc cgaatctctt cacccgctac tggcaagcgg aacgcagcaa gcgccgttcc
     5341 ggcggcagcg ggctcggcct caccatcgtc aagcaactcg tcgaggcgca ccagggcgag
     5401 atcacggtcg agagccaggt tggccagggg acgaccttcc gcttcacgat cccgcagcga
     5461 taatcccgtg ggcgaggcgc tcgcctttcc gacatcacga cggagtaacc cttctgatga
     5521 agatcatcca ggcaggcatc ggcg
//
LOCUS       DASTMD010000335         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_477540, whole genome shotgun sequence.
ACCESSION   DASTMD010000335 DASTMD010000000
VERSION     DASTMD010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477540"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..505)
                     /locus_tag="VFQ54_04905"
     CDS             complement(194..505)
                     /locus_tag="VFQ54_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164362.1"
                     /translation="MASTVVRVEETTHAKLNAWSEAQKKPISQIVRELVDHQEAELFW
                     AAMHEDFDRLRADPVAWKDYQDEARIFEGGSMDGLEDDEPYYTVEEEAEIEKHARSQG
                     W"
     gene            complement(602..1096)
                     /locus_tag="VFQ54_04910"
     CDS             complement(602..1096)
                     /locus_tag="VFQ54_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0164363.1"
                     /translation="MANDPLTIRPATSTDRAAVARIADPVQDDHARALPARFRPAVSP
                     LPMEHFHQLLTSRISGILVAEAGGDIVGFVVLRVTDASPIPVYQPRRVAFIDMIAVDQ
                     TCRGQGIGKALMEAAMEWGRERSADELELSVHEFNTSAIAFYEHLGLRTVLRRMSVPL
                     HLAD"
     gene            complement(1112..1915)
                     /locus_tag="VFQ54_04915"
     CDS             complement(1112..1915)
                     /locus_tag="VFQ54_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0164364.1"
                     /translation="MTTGKIRDGRMVAINGADLHVREIGQGQPIVLVQPGLLSGECYE
                     QLATLLAARFRVVTFDSRGHGRSTNPGGVLTYELLADDTVELIRALGMERPFVGGWSD
                     GGEVALQVGLRHPGLARGLIAGGTSLEMGGSERDLAGLRAFFCTGANDVVDVDAFATR
                     WEGSLLPMLRESHPGGEAQWQDVVRWSARMWMTYVGLTREETARITAPTLVAIGDRDE
                     FVPVEEGVRLYRWLPNAGLAILPGSNHMRPVFEPASFASVLTDFVDRHS"
BASE COUNT          387 a          684 c          581 g          356 t
ORIGIN      
        1 tgtcttcgtc aatcccccac cgggggcatc aacacgcaca tggccgcgga ttccgcgatc
       61 tgtcccggta atcggcacaa caattcggaa ggtatgcggt acgttgttga actggtcgct
      121 tgagataacc agagcaggac gtattccgcc ctgctcgtgc cctacctgtg gcgaaaaatt
      181 gacatcccag atctcaccag ccctgggatc tggcgtgctt ctcaatctcg gcctcctctt
      241 ccaccgtgta atacggctcg tcatcctcaa gaccatccat tgaaccaccc tcgaagatcc
      301 gggcctcgtc ctgatagtcc ttccaggcaa ctggatctgc gcgcaagcga tcgaaatcct
      361 cgtgcatcgc cgcccagaag agttcggctt cctggtggtc aacgagctcc cggacaatct
      421 ggctaatcgg cttcttctgc gcttcggacc acgcattgag cttcgcatgc gtcgtttcct
      481 cgacacgcac gaccgtcgat gccatgtttg ccctcctcct cgcatcgtat ttcagagcat
      541 tctgttacat ccaagtatac attgatgtaa acatgagctt aagaagacgc gagttcgccg
      601 atcagtccgc gaggtgcaga ggaacggaca tccgccggag caccgtgcgc aggccgaggt
      661 gctcgtagaa ggcgatcgcc gacgtgttga actcatgcac gctcagttcc agctcgtcgg
      721 cggaccgctc ccgaccccat tccatcgcgg cctccatcag cgccttcccg atcccctgcc
      781 cacggcatgt ctgatccacg gcgatcatat cgatgaaagc gacccggcgc ggctgataca
      841 cggggatcgg gctggcatcg gtgacccgga gcacgacgaa acccacgatg tctcccccag
      901 cctccgcgac caggattccg gagatccgcg aggtgagaag ctgatggaaa tgttccatcg
      961 gcaacggcga gacggcggga cgaaaccgcg cgggcagcgc ccgggcatgg tcgtcctgga
     1021 caggatcggc gatgcgggca accgccgcgc gatcagtact cgtagctggt cgaatcgtca
     1081 gcggatcgtt tgccatggct ccatctccgg gtcaggaatg ccgatcgacg aaatcggtga
     1141 ggacagacgc gaagctcgcc ggctcgaaga ccgggcgcat gtgattgctg ccggggagga
     1201 tcgccagccc cgcgttcggc agccagcgat agaggcgaac tccctcctcg accgggacga
     1261 attcgtcgcg gtcaccgatc gccaccagcg tcggcgcggt gatacgcgcc gtctcctcgc
     1321 gcgtgagtcc cacatacgtc atccacatcc gggccgacca gcggacgaca tcctgccact
     1381 gcgcctcgcc accgggatgc gactcccgca gcatcggcaa gagcgatccc tcccatcgcg
     1441 ttgcaaacgc atcgacgtcg acgacatcgt tcgcgccggt gcaaaagaac gcgcgcaacc
     1501 cggcgaggtc gcgctcgctg ccacccatct ccaggctcgt gccgccggcg atcaggccgc
     1561 gcgcgagacc cggatgccgc aaccccacct gcaaggcgac ctccccgcca tcgctccagc
     1621 caccgacgaa cggccgctcc atgccaagcg cccggatcag ttcgacggtg tcgtcggcga
     1681 gcagctcata cgtcaacaca ccgccgggat tcgtcgatcg accatgcccc cggctgtcga
     1741 aggtgacgac gcgaaaccgc gcggccagga gtgtggcgag ctgttcgtag cattcgccgg
     1801 aaaggagccc gggctgcacg agcacgatcg gttgcccttg cccgatttcc cgaacgtgga
     1861 gatcggcacc attgatggcg accatccgac cgtctctgat cttcccggtt gtcacatcag
     1921 gctccttcgc tccgagaatt tcgcggccag atgcggcggc ggcgactatc gcgatgatcc
     1981 gcgatcaggg gccgggcgtc gagcgatc
//
LOCUS       DASTMD010000336         1909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1370071, whole genome shotgun sequence.
ACCESSION   DASTMD010000336 DASTMD010000000
VERSION     DASTMD010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370071"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="VFQ54_04920"
     CDS             <1..1345
                     /locus_tag="VFQ54_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HEU0164365.1"
                     /translation="PGLQDAIEGHLRRVEERIGQWADVVSGGLIDTAQAGADAGESFA
                     REDVDLLLCYMGTYATSTIVLPVVQRAAVPVILLNLQPVAALDYADVDTATWLANAGV
                     CAVPEFAGVFLRSAIEFDVVTGTLDDDPIAWEEVRSWCVAAGVARTIRRARFGMLGHT
                     YPGMLDMSTDVAALQAQLGAHIEILEIDDLKDLVERATSEEVAAIVARTGDLFVLEGD
                     ISQEAITWGATVAAGLQDLAADFDLDGLAYYYRGTGGDRLEQIAANMILGNTLLTAEG
                     IPAAGEGDLKTAIAMKILDELGAGGSFTEFAAMDFQDDFFLMGHDGPAHPGIGDGKPT
                     LKELAVFHGKSGGGLAVEIRARTGPVTVLGVTQTTEGRLKLIAAEGESLPGDALRLGN
                     SNHRIRFGQDVRAFMRAWCALGPTHHVALGLGHRIADLQRVATLLDIPFERVGPE"
     gene            1440..>1909
                     /locus_tag="VFQ54_04925"
     CDS             1440..>1909
                     /locus_tag="VFQ54_04925"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HEU0164366.1"
                     /translation="MSDRMHQCVSRRTLVAGSSAIAATALTRVAVDAQGTPAASPVPA
                     SDLPLAQVAILTGSDPAAVNQTGSLYEVDGTDLGSSFLFHDEIVLAFGDTFAEFKTDW
                     RSNTLAISTDDDPSDGIVFDRMIVDRPGHAKELLASKKEDFVEMTVIPTYGVAVG"
BASE COUNT          324 a          594 c          657 g          334 t
ORIGIN      
        1 cccgggattg caggacgcga tcgaggggca tctgcgccgc gtggaagaac ggatcgggca
       61 gtgggccgat gtcgtcagcg gcgggctgat cgacacggcc caggcgggcg cggacgcggg
      121 agaaagcttc gcgcgggaag atgtcgatct gctcctctgc tacatgggga cctacgccac
      181 gagcacgatc gtgcttcccg tcgtccagcg cgccgcggtg cccgtgatcc tcctcaacct
      241 gcagccggtc gcggcgctcg attatgcgga tgtggatacc gcgacctggc tggcaaacgc
      301 gggtgtctgt gccgtgcccg agttcgccgg tgtcttcctt cgttccgcga tcgagttcga
      361 cgtcgtgacc ggtacgctcg acgacgatcc gatcgcgtgg gaggaggtgc ggtcctggtg
      421 cgtcgcggcg ggtgtggcgc gtacgatccg ccgtgcgaga ttcgggatgc tcgggcacac
      481 ctatcccggc atgctcgaca tgtcgacgga cgtcgcagcc ctgcaggccc aactcggtgc
      541 gcatatcgag attctcgaga tcgacgacct gaaggatctg gtcgagcggg cgacgtcgga
      601 ggaggtcgcg gcgatcgtgg cgcgaacggg ggatctcttc gtgctcgaag gcgacatatc
      661 ccaggaagcg atcacctggg gcgcaaccgt ggccgccgga cttcaggatc tggccgcgga
      721 tttcgatctc gacggacttg cctactatta tcgcggcact ggcggcgatc gcctcgagca
      781 gatcgcggcg aacatgattc tcggcaacac gctccttacg gcggagggca ttccggcggc
      841 aggggagggc gatctcaaga cggcgatcgc gatgaagatc ctcgatgaac tcggtgccgg
      901 gggatccttc acggagttcg cggcgatgga cttccaggac gacttcttcc tgatgggcca
      961 cgatggtcca gcgcaccccg gaatcggcga cggcaagccc acgctcaagg aactcgccgt
     1021 ttttcatgga aagtccggcg gcgggcttgc cgtcgagatt cgcgcccgga cggggccggt
     1081 caccgtgctc ggagtcaccc agacgaccga ggggcgcctc aagctgatcg ccgcggaggg
     1141 agagagcctc ccgggagatg cgctgcggct cgggaactcg aatcatcgga ttcgattcgg
     1201 gcaggacgtc cgtgcgttca tgcgtgcctg gtgcgcgctc gggccaacgc atcacgtcgc
     1261 gctcggtctc ggtcaccgca tcgcagacct gcagcgcgtc gcgacgctgc tcgacatccc
     1321 gttcgagcgt gtgggcccgg agtgaacgag gaaaatcggg accgagcctg gccgggcgtc
     1381 gcggcgttga tcgccggatc gagcacgacg ccgggctaca tcgtgaaggg accagacaga
     1441 tgtcagaccg aatgcatcaa tgtgtctcgc gtcgcaccct ggtggcgggc agttcggcaa
     1501 tcgccgcgac ggcgctgacg cgcgtggcgg tcgacgcgca ggggactccc gcggcgtctc
     1561 cggtaccggc aagcgacctg ccactggccc aggtcgccat cctcaccggc tcggatcccg
     1621 ccgcggtcaa tcagacgggg tcgctctatg aggtcgatgg tactgacctc ggcagcagct
     1681 ttctctttca tgacgagatc gtgctcgcct tcggcgatac cttcgccgag ttcaagaccg
     1741 actggcgaag caatacgctc gcgatttcaa ccgacgacga cccatccgat ggcattgtct
     1801 tcgatcgcat gatcgtcgat cgtcccggcc atgcgaagga actcctcgcg tcgaagaagg
     1861 aagacttcgt ggagatgacg gtgatcccga cctacggcgt tgccgtggg
//
LOCUS       DASTMD010000337         4248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1818397, whole genome shotgun sequence.
ACCESSION   DASTMD010000337 DASTMD010000000
VERSION     DASTMD010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818397"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFQ54_04930"
     CDS             <1..293
                     /locus_tag="VFQ54_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164367.1"
                     /translation="LKQGFDVAIVCRGKAGELPGLTEAQEALKRIFTKAALFDPAAHT
                     EDPPGPPPPGSPILTGWSRPRQEPERADPPHEGTGLTGNSEERDGFDTFGLT"
     gene            380..589
                     /gene="yidD"
                     /locus_tag="VFQ54_04935"
     CDS             380..589
                     /gene="yidD"
                     /locus_tag="VFQ54_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU0164368.1"
                     /translation="MKRIALAMIRFYRRGISPNLPPSCRFQPTCSEYAYEAIEKYGIM
                     KGGRLATWRILRCNPWGGKGWDPVP"
     gene            979..2298
                     /locus_tag="VFQ54_04940"
     CDS             979..2298
                     /locus_tag="VFQ54_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEU0164369.1"
                     /translation="MLFDVLGSLFSLNGLFVGQFQHLITTMPLGSIPVWDQYIDLLKW
                     ILNHLGDAFSNGGLAIIAFTIIIKTLLLPLTVKSIRSSKNMQELAPKIKDIQKKYGKD
                     RQRASQETMALYQQHGVNPMAGCLPMVLQIPIFFGLYHTISNLSKSGTGVWDGGFLWL
                     SSLDHPDPYKILPIMAGVFQFVQARMMRPAGQKVTDPQQQIMNTMMNFMPLMVVLFGW
                     NFSSGPVIYWVTQAIYSVVQQWMITGWGSFGEWFPWLPELPAHRRLGYQDPSKLRDVV
                     VMSGEAGVVQQKGISGWLNRKMMEAQETAQERQKAQQEARGGSKSGSKAGSTSSTSRN
                     GKASTANRNTRANAGKTTAKAEVVEEGDDDAVEEVINDRVTRRQSNYQSRVAGSKFGA
                     TRGGATTATAAAENGDGGDTAPVTTATSSGPRRTGKSRTKPQRRPTT"
     gene            2518..3390
                     /gene="jag"
                     /locus_tag="VFQ54_04945"
     CDS             2518..3390
                     /gene="jag"
                     /locus_tag="VFQ54_04945"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEU0164370.1"
                     /translation="MQQRTSVEIQAYSVDEAVRLALEELGLTQDQVDIEILSDAGPDE
                     DAEALVRVTARGMASQPVPTSGPRVTAPGTTTAPGERNGRNRRRGRGGSGNGDSAGGS
                     DWRATRSGPGDRPSQRGTPPGVPAVDRADADDERVAKEVVGELLTHMGVHADVMAVDN
                     PSAVPLDEEEPPTIFIDVLGSDLGMLIGRRGDHLAQIQYLVNMVVNRRIETWTRIVLD
                     VEGYRSRREESLVALAERVARQVIRSHRPIALEPMPPNERRVIHLTLRQHPEVTTQSS
                     GEGNQRHITVEPRA"
     gene            3569..>4248
                     /locus_tag="VFQ54_04950"
     CDS             3569..>4248
                     /locus_tag="VFQ54_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164371.1"
                     /translation="MTLEKIVTEKTSANTANGATPAKKNASRESFQTERRYSGTQLSR
                     TPDYLLIGHITADLQADGSVVLGGTALYSALTAARLGARVGILTRGNFGTEVGGMTIP
                     SLEPYADQISIIVQEAEFPTTFINEYRADLRVQTIRHWAGEIDLRGLPPHWRNAPIVH
                     LGPVADEVDPYQAVGLTTDFIGITPQGWMRDWPRQTGGKVTHIPLKLPGSLLSRVDCA
                     IVSVEEIT"
BASE COUNT          818 a         1416 c         1289 g          725 t
ORIGIN      
        1 acctcaagca gggcttcgac gtcgccatcg tctgccgggg caaggccgga gagctgcctg
       61 gtctcaccga ggcgcaggaa gccctgaagc gcatcttcac gaaggccgcg ctcttcgatc
      121 ccgccgccca cacggaggat ccgccgggac cgccaccgcc aggaagcccg attctcaccg
      181 gctggtcgcg gccgcgccaa gagccggaac gggcggatcc cccgcacgaa ggaaccggac
      241 tcacaggaaa ttcggaagaa cgtgacggtt tcgacacatt cggtttgacc tgaggcgtat
      301 catttccttg acaccttccg atcatccgtc tcaacatcgt cgcccgtccc ttcgttcgtc
      361 gccgtggtcg tgatcatcaa tgaagcgcat cgccctcgcc atgatccggt tctaccggcg
      421 tggcatctcg ccgaatctcc cgccatcgtg ccgcttccag cccacgtgct ccgaatatgc
      481 ctacgaggcg atcgagaagt acggtattat gaaaggcgga cgtctcgcga cctggcggat
      541 tcttcgctgc aacccgtggg gcggcaaagg ctgggatccc gttccataag aacatcgcag
      601 gctgggcatc cccacattca tcccgtcgat cgggagattc ccttcaatca gccaggtgga
      661 tggtcagttc cagccgacag gtcgtgcatc agattcccag cgaaagttgg ttttttcgcg
      721 gcgaatcgat aatcatcatg gatgcatagt gacaatcggt cacggcaccc gtgcatatca
      781 gttccggtcg gatcgtgggt cgttctttcg tccccccgct tacccgaatc gaccaaccgg
      841 acacatgccg cctgtcgcgt cgtttttgct ctcgatctgt ccgggaatta ccccggattc
      901 cggtatcgtt cgattcgctt cgcggaacgg cgatacccat aaccgcagtc ccgcccaggc
      961 gggaggaggc cttccctcgt gctcttcgac gttctcggat cgttgttctc gctcaacggc
     1021 ctcttcgtcg gccagttcca gcatctgatc acaacgatgc cgctcgggtc gatccccgtt
     1081 tgggaccagt acatcgatct cctcaagtgg attctcaacc acctgggcga cgccttcagc
     1141 aatggtggtc tggcgatcat cgccttcacg atcatcatca agacgctgct gctgccgctg
     1201 accgtgaaat cgatccgctc cagcaagaac atgcaggagc tcgccccgaa gatcaaggac
     1261 atccagaaga agtacggcaa ggatcgccag cgcgcctccc aggagacgat ggcgctctat
     1321 cagcagcacg gcgtcaaccc gatggccggc tgtctgccga tggtgcttca gatcccgatc
     1381 ttcttcggtc tctaccacac gatctcgaat ctctcgaaga gcggtaccgg cgtctgggac
     1441 ggcggcttcc tctggctctc cagtctcgat catccggatc catacaagat cctgccgatc
     1501 atggccggtg tcttccagtt cgttcaggcc cgcatgatgc gcccggccgg gcagaaggtg
     1561 accgatccgc agcagcagat catgaacacc atgatgaact tcatgccgtt gatggtcgtg
     1621 ctcttcggct ggaacttctc ctccggtccg gtcatctact gggtgacgca ggcaatctac
     1681 tccgtcgtcc agcagtggat gatcaccggt tggggttcct tcggcgagtg gttcccatgg
     1741 ctgccggaac tcccggcgca ccgccgcctc ggctaccagg atccctccaa gctgcgtgac
     1801 gtcgtcgtga tgtccggaga ggcaggcgtg gttcagcaga agggcatctc cggctggctc
     1861 aaccgcaaga tgatggaagc gcaggagacc gcccaggagc gccagaaggc gcagcaggaa
     1921 gcgcgaggtg gctccaagtc gggcagcaag gcgggttcaa cctcgtccac gagccgcaac
     1981 ggcaaggcga gcaccgcgaa tcgcaacacc agggcgaacg ccggcaagac caccgcgaag
     2041 gcggaagtgg tcgaagaagg tgacgacgat gccgtcgaag aggtgatcaa cgaccgtgtc
     2101 accaggcggc agtcgaatta ccagtcccgc gttgcggggt cgaagttcgg cgcaacccgc
     2161 ggcggagcaa cgacggcaac ggccgccgcc gagaatggtg atggcggaga caccgcgccg
     2221 gtgacaaccg caacctcctc cgggccgcgg cgaacgggca agtcccgcac gaagccacag
     2281 cggcgaccga cgacctagcg cgggccgcgg ctcgcgaccc aggacatagt tgattcgaca
     2341 ggaattcgac cgaagtggcc acccagcgtg gccgcttcgt ctggcgccat gtaccatgca
     2401 ggtcacacga tagcgatcat gtacgaaccc gccagtacga ccatttcatg attcttctca
     2461 agaatcctcg tgccctgagc tgacacgggg tcgcgagcgc aagaggaagc cgggcagatg
     2521 cagcaacgaa ccagtgtcga gatccaggcc tattcggttg atgaagccgt gcgcctggca
     2581 cttgaagaac tcgggctcac gcaggatcag gtcgatatcg agatcctctc cgacgccggg
     2641 ccggatgagg acgccgaagc gctcgttcgc gtgaccgccc gtggcatggc gtcgcagccc
     2701 gttccgacga gtggcccccg cgtgaccgcc cccgggacga cgaccgcgcc gggagagcgc
     2761 aacggtcgca accggcgccg cggtcgcggt ggaagtggca acggtgattc cgccggcggc
     2821 tcagactggc gcgcgacgcg aagcggaccc ggcgatcgcc cctcccagcg cggcacccct
     2881 ccaggggttc ccgccgtcga ccgtgcggac gcggatgacg agcgcgtggc gaaggaggtt
     2941 gtcggcgagt tgctgacgca catgggcgtc cacgccgatg tgatggcggt cgacaatccc
     3001 tccgcagtac cgttggatga agaagaaccg ccaacgatct tcatcgacgt gctcggcagc
     3061 gatcttggca tgctgatcgg acgtcgcggc gatcatctcg cgcagatcca gtacctcgtc
     3121 aacatggtcg tcaatcgtcg gatcgagacc tggacgcgga tcgtcctcga tgtcgagggc
     3181 tatcgctccc gtcgcgagga gtcgctcgtc gcgctggccg agcgcgtcgc ccgtcaggtc
     3241 atccgcagtc atcgcccgat cgccctcgag ccgatgccgc ccaacgagcg ccgcgtgatc
     3301 cacctcacgc tgcgtcagca ccccgaggtg acaacgcaga gcagtggcga gggcaaccag
     3361 cggcatatca ccgtcgagcc gcgagcctga cctaacctac gagatcgacc ggactctccc
     3421 gcccgaaatg cgggaaacgg cgcgccgcag acacggcgtc gccacgttac caatcgattg
     3481 ttccggcgcc ggacggcgtc gactggagag ccctcccgcc gcttcgatgg cgccggatcg
     3541 ggtgttcgca tgacgatcat gcagatgcat gaccctggag aagattgtga ccgagaagac
     3601 atccgcgaac accgccaacg gcgccacgcc agcaaagaag aacgcctcgc gcgaatcctt
     3661 ccagacggag cggcgatata gcggcaccca gctctcccgg acgccggatt acctccttat
     3721 cgggcacatc acggccgatc tccaggccga tggcagcgtc gtcctcggcg ggaccgcgct
     3781 ctattccgcc ctgaccgcgg cccgcctcgg agcacgcgtc ggcatcctga cgcgcggcaa
     3841 cttcggcacg gaagtcggcg gcatgacgat cccgtcgctc gagccctacg ccgaccagat
     3901 cagcatcatc gtgcaggaag ccgagttccc gaccaccttc atcaatgagt accgcgccga
     3961 tctccgcgtc cagaccatcc ggcactgggc gggtgagatc gatctccgcg gcctcccgcc
     4021 gcactggcga aacgcgccga tcgtccatct cggtccggtc gcggacgagg tggatcccta
     4081 tcaggccgtc ggcctgacga ccgacttcat cgggatcacg ccgcaaggct ggatgcgcga
     4141 ctggccgcgc cagactggtg gcaaggtgac gcacattccg ctcaagctgc cgggatcgct
     4201 gttgagccgt gtcgactgcg cgatcgtcag cgtcgaggag atcaccta
//
LOCUS       DASTMD010000338         1561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2085249, whole genome shotgun sequence.
ACCESSION   DASTMD010000338 DASTMD010000000
VERSION     DASTMD010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2085249"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /gene="mgtE"
                     /locus_tag="VFQ54_04955"
     CDS             <1..956
                     /gene="mgtE"
                     /locus_tag="VFQ54_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU0164372.1"
                     /translation="AGALLTPEFVAIAPNLRAEQAIMALRAIAADAEVVNYVYVLDRE
                     DHLLGVLSLRNLVLAQPDAPVRDLMIAPAVATSVTTDVQDAANLLRDRNLLALPVVDD
                     DNRLLGVITHDDAIEVLEDELTEDYLKMAGTDADEMSRRNPFEIARLRLPWLMGTMGL
                     ELFAGLVINNFSHVLQQVILLASFMPVISAISGNVGLQAAAIVVRGLDTGHVTLADWR
                     RHLGRELSTSLIMALSCGIVLGSIGAVWSRHLPFGLVIGGALTCSMLTAGLMGTLIPM
                     LSKRLGFDPATTAGPFETAFQDVIGFAVFLWLASLLINFIS"
     gene            1097..>1561
                     /locus_tag="VFQ54_04960"
     CDS             1097..>1561
                     /locus_tag="VFQ54_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU0164373.1"
                     /translation="MNVLILGGTGFIGPHVVRHLVAGGHDVTLYNRGNSTANLPAGVG
                     HIPGDRSDVASHADDFRAVSPDVVLDMRSMLESEARRIVETMIGIAPRIVTISSMDVY
                     KAFGVLIGTETGSVAVPFDENGPLRENSYPYRTTEPIAPDDPSYWRQDYDKIP"
BASE COUNT          259 a          518 c          469 g          315 t
ORIGIN      
        1 cggccggggc cctgctgacg ccggagttcg tcgccatcgc gccgaacctc cgcgccgaac
       61 aggcaatcat ggcgttgcgc gcgatcgccg ccgatgccga ggtcgtcaac tacgtctatg
      121 tcctcgatcg cgaggatcat ctgcttggcg tactctccct gcgcaacctc gttctcgccc
      181 agccggacgc cccggtgcgt gacctgatga tcgcgccggc ggtcgcgaca tccgtcacca
      241 cggatgtcca ggatgccgcg aacctcctgc gtgaccgcaa ccttctcgcg ctcccggtcg
      301 tggacgacga caaccgtctc ctcggcgtga tcacccacga cgacgcgatc gaggtcctcg
      361 aggatgagct gaccgaggat tacctgaaga tggcgggtac cgacgccgac gagatgtcgc
      421 gccggaatcc cttcgagatc gcgcgcctcc gcctcccgtg gctgatgggt acgatgggtc
      481 tcgaactgtt tgccggactc gtgatcaaca acttctcgca cgtcctccaa caggtgatcc
      541 tgctcgcctc gttcatgccg gtgatctcgg cgatctcggg gaacgtcggc ctccaggcag
      601 ccgcgatcgt cgtgcgtggg ctcgataccg gacacgtgac gttggccgat tggcgccgtc
      661 acctcggacg cgagctctcg acatcgctga tcatggcgtt gagttgcgga atcgtgctcg
      721 ggagtatcgg cgccgtctgg tcccggcact tgccgttcgg tctcgtgata ggcggcgcgt
      781 tgacctgttc catgctgacc gccggtctga tgggcaccct gatcccgatg ctctcgaagc
      841 ggctcggctt cgacccggcg acaaccgccg gtccgttcga gaccgcgttt caggacgtga
      901 tcggcttcgc ggtcttcctc tggctcgcct cgctgctgat caacttcatc tcctgacgac
      961 caacccgtcg ctgccacaag agtgaccatc ccgtttggat gaccgatcta tcgcccggat
     1021 ctcatatctg cgcgagaatc gggtctcatt gagaatgatc gatgcgattc cccattagac
     1081 gaggagtatt cgaaggatga acgtcctgat tctcggcggc accggtttca tcggcccgca
     1141 cgtcgttcgt catctggtcg ccggcggcca tgacgtgacg ctctacaacc gtggcaactc
     1201 caccgcgaat cttcccgcag gggtcggtca tatcccgggt gatcgctcgg atgtcgccag
     1261 ccatgccgat gacttccgcg cggtctcgcc ggatgtcgtg ctcgacatgc ggtcgatgct
     1321 ggaatcggag gcacggcgga tcgtcgagac catgatcggc atcgcgccga ggatcgtcac
     1381 gatcagcagc atggacgtct acaaggcatt cggcgtcctg atcgggacgg agaccggaag
     1441 cgtcgctgtc cccttcgatg agaacgggcc gctccgtgag aactcctacc cgtaccgtac
     1501 gaccgagccg attgccccgg acgatccatc ctattggcgc caggattatg acaagattcc
     1561 g
//
LOCUS       DASTMD010000339         1737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1728347, whole genome shotgun sequence.
ACCESSION   DASTMD010000339 DASTMD010000000
VERSION     DASTMD010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728347"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1737)
                     /locus_tag="VFQ54_04965"
     CDS             complement(<1..>1737)
                     /locus_tag="VFQ54_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164374.1"
                     /translation="DFSLALSDDGQTYRTVLTGSLSIVATEQSFVLDKPVTARYARLT
                     ILSAQSSNSTTATIGEWKVIAQPGWSPPQIGGTPAASGIDIASTDVGGHVVATNPQLD
                     LNIGTQGIVTADQQRQTINVVAGAPVSWVIGFNDNRAAQITSMTWQDPPGSDSTVRFD
                     SVQVDVSTESPTGPWTSVGTWTLNRSNGSPTYAFDTPTWARFVQFTGVTPPDQATPAA
                     SSPTTLAWELPDDIAIDERGQDATYRSILGEWGAYSQDAVYERLVPPAAIQLDPDAGN
                     DQASATLLPIGSRITNTAAVQTDEDWYRVDVPAGMGQLDFTLTGDPTLDVSAVLADAS
                     GKTIDMQENAASASETRYTATVSGGASYYLRVTQPPTSVVFAFDTSGSIGPYVSTVYQ
                     GLGQYAGEVQPGKEVVNFVPFGEKLLLDDWSGDPYLLQGAIAGYPRTAGSSDSEGSML
                     TAMDALSGRDGTKAIVLITDAESSPSQDEIDKLWPELGTVKPRIFTIQIAGNGGLQHS
                     QDLMQDWASVNNGQYVYVRDQGEMDVAFERAAAALRRPTVYTVSVSASAPKPTPTPRP
                     TNTPVPTATPSPT"
BASE COUNT          273 a          547 c          586 g          331 t
ORIGIN      
        1 gtcggactcg gcgtggcggt tggcaccggc gtgttcgtcg gtcgcggagt cggggtcggt
       61 ttcggcgcag acgcggagac gctcaccgtg tagacggtgg gtcgcctcag ggccgccgcc
      121 gcgcgctcga aggcgacgtc catctctccc tggtcgcgaa cgtagacgta ctgcccgttg
      181 ttgacggacg cccaatcctg catcaggtcc tgcgagtgct ggaggccgcc gttgccggcg
      241 atctggatcg tgaagattcg cggcttcacg gtgccgagtt caggccagag cttgtcgatc
      301 tcgtcctgcg acggcgacga ttcggcgtcc gtgatcaaca cgatcgcctt cgtcccgtcg
      361 cgcccggaga gggcgtccat cgccgtgagc atggatccct cggagtcgct cgatcccgcc
      421 gtccgtggat acccggcgat cgctccctgg agaagatagg ggtcaccact ccagtcgtcc
      481 agcaggagtt tctcgccgaa cggcacgaaa ttgacgacct ccttgcccgg ctgtacttct
      541 cccgcatatt gaccgagtcc ctggtagacg gtggacacgt aagggccgat gctcccgctc
      601 gtgtcgaagg cgaagacgac ggacgtcgga ggctgcgtca cccgaaggta gtacgaggcg
      661 cctccgctca cggtcgcggt atagcgagtc tcgctcgccg atgccgcgtt ctcctgcatg
      721 tcgatcgtct tgccgctcgc gtcggcaagc acggcgctga cgtcgagcgt cggatcaccg
      781 gtcagcgtga aatcgagctg ccccattccc gccgggacgt cgacgcgata ccaatcctca
      841 tcggtctgca cggccgccgt gttcgtgatc cgggagccga tcgggagcaa cgttgcgctg
      901 gcctggtcgt tgccggcatc cggatcgagc tggatcgcgg caggaggcac cagacgctcg
      961 tagaccgcgt cctggctgta ggcaccccat tcgccgagaa tcgagcgata ggtggcatcc
     1021 tggccacgtt catcgattgc gatatcgtcg gggagttccc acgccagggt tgttggtgag
     1081 ctggccgccg gcgtcgcctg atctggcggc gtgacaccgg tgaactggac gaatcgcgcc
     1141 caggttggcg tgtcgaaggc gtaggtcgga ctcccgttcg accgattgag cgtccaggtt
     1201 ccaacggatg tccacggccc ggtcggactc tcggtgctca cgtcgacctg tacggaatcg
     1261 aatcgaaccg tggaatcgct gccgggcggg tcctgccagg tcatcgacgt gatctgggca
     1321 gcgcgattgt cgttgaatcc gatcacccac gagaccggcg cccccgccac gacattgatg
     1381 gtctgccgtt gctggtcggc ggtcacgatc ccctgggtac cgatgttgag atcgagctgg
     1441 ggattcgtcg cgaccacgtg gccgccgacg tccgtcgatg cgatgtcgat cccggaagcg
     1501 gcgggtgttc cgccaatctg tggcggagac catcctggct gggcgatgac cttccattcg
     1561 ccaatcgtgg ccgtggtgga gttcgaggat tgcgcggaga gaatcgtcag tcgcgcgtac
     1621 cgggcggtca ccggcttgtc gagcacgaat gactgctccg tcgcgacgat cgagagcgat
     1681 cccgtcagga cggtccggta cgtctggcca tcgtccgaga gcgcgagcga gaagtcc
//
LOCUS       DASTMD010000340         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_568298, whole genome shotgun sequence.
ACCESSION   DASTMD010000340 DASTMD010000000
VERSION     DASTMD010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568298"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1631)
                     /locus_tag="VFQ54_04970"
     CDS             complement(318..1631)
                     /locus_tag="VFQ54_04970"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0164375.1"
                     /translation="MSGIREPADRVEIAIAEVHRTGWAHVLGSVVHFTRDLDLAEDMV
                     QDAFVAALDTWPREGIPANPAAWLITTAKRKALDRIRRQATFERKLPLLVVPEGEDEG
                     DDMDGDHIPDERLRLIFTCCHPALSLEARVALTLRLVCGIQTPDVARLLLVSEATMAA
                     RITRAKKKIAIAGIPYRVPSAVELVDRLPAVLGVIYLLFTEGHTAGRGDRLVQDDIIE
                     RSLRLATTLVELLPDEGEVLGLLALIRLTDARRHTRVDAAGELVLLADQDRTRWDRTG
                     IEDGLRVTERALRLSAGRRPGRYAVQAAIAAVHAEPVNAADTDWAELVALYDLLLVID
                     PSPVVALSRAVAIGERDGAAHGMMALAAVEDHPALVRYHMLPALRADFLRRLGRDDDA
                     LLAYDQALNLTGNAVEQSFLRRRIAELHARGTRPRGACHRSGTVS"
     gene            complement(1653..2003)
                     /locus_tag="VFQ54_04975"
     CDS             complement(1653..2003)
                     /locus_tag="VFQ54_04975"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0164376.1"
                     /translation="MKYMLIIGNESSPWWNEQDPAAMGETGKAHQEFAAWCAERGTAI
                     VSGAALGSPATAKTIRQQDGEFLITDGPHADLKESIGGFYVVDVAGFDDALEVARHCP
                     MDPRYGIEVRTVEM"
     gene            complement(2068..>2312)
                     /locus_tag="VFQ54_04980"
     CDS             complement(2068..>2312)
                     /locus_tag="VFQ54_04980"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0164377.1"
                     /translation="DANGVAYSGGSALFGPDAARTVRKKNGEFVVTDGPYADLKEVVG
                     GFYVIDAPDAAAAAEIAKHCPMDSDSGIEVRPFWEH"
BASE COUNT          406 a          775 c          766 g          365 t
ORIGIN      
        1 cgatccatgc gcggcactcc ccgaatcccg agcggcacgg cgcgtccttc ccaccattgg
       61 gcgatcatca gggcctgtcc tgggcgcagc cgaaggaatc gcccccaggc gatcgtcacg
      121 gcgcggcacg tttcggccac gttggggagg cgaccacacg ggtcgccggg acgaatgaat
      181 cagtgatgcg ggctcccagc gcaacacgtc gacccggaat ggcgacaagc gccatgggga
      241 cggtgccggg gttgtcgagg ccggacacgg agagcttgcc ttggcagccg catcggaatg
      301 cagccgaagg aatcgcccta cgagaccgtg ccggagcggt gacaagcgcc gcggggacgg
      361 gtgccgcggg catggagttc ggcgatgcgg cgccggagga agctctgctc gacggcgttg
      421 ccagtgaggt tcagcgcctg gtcataggcg agcagcgcat cgtcatcgcg gccaaggcgt
      481 cgcaggaagt ccgcacgcag ggccggcagc atgtggtagc gaacgagcgc gggatgatct
      541 tccactgccg ccagcgccat catcccgtgc gcggcgccgt cacgctcgcc gatcgccacg
      601 gcgcggctga gcgcgacgac gggtgaggga tcgatcacca gaaggagatc atagagggcg
      661 acgagttcgg cccagtcggt atccgcggca ttgacgggct ccgcgtgaac ggccgcgatt
      721 gccgcctgca ccgcgtagcg acccggacgc cgccccgccg agaggcgcag cgcccgctcg
      781 gtcacgcgca atccgtcctc gatccccgtt cgatcccagc gcgtacggtc ctgatccgca
      841 aggagtacga gctcgccggc agcatcgacc cgcgtgtggc gtcgtgcatc agtgaggcgg
      901 atcaatgcga gcagcccaag cacctcgccc tcgtccggga gcagctcgac gagcgtcgtc
      961 gccagccgga gcgagcgctc gatgatgtcg tcctgcacca ggcggtcgcc gcgaccggcg
     1021 gtgtgcccct ccgtgaagag gagatagatc acgccgagga cggcgggaag gcgatcgacc
     1081 agttccaccg ccgacggcac ccggtagggg atgccggcga tggcgatctt cttcttcgcg
     1141 cgggtaatcc gggccgccat cgtcgcctcg ctcacgagca ggaggcgcgc gacatccggt
     1201 gtctggatcc cgcagacgag gcgaagcgtc agggcaacgc gcgcctcgag ggagagggcg
     1261 gggtggcaac aggtgaagat caaccgcaac cgttcatcgg ggatgtggtc cccatccatg
     1321 tcatcacctt cgtcctcccc ctcgggcacg accagaagcg ggagcttgcg ctcgaacgtc
     1381 gcctggcggc gaatgcgatc gagcgccttg cgtttcgccg tcgtgatcaa ccaggcggcg
     1441 gggttcgcgg gaatgccttc ccgcggccag gtatcgagcg cggccacgaa cgcgtcctga
     1501 accatgtcct cggcgagatc gagatcgcgc gtgaagtgca cgaccgaacc gaggacatgc
     1561 gcccacccgg tacgatgcac ctccgcgatt gcaatctcga cgcgatcagc cggttctcga
     1621 atcccgctca tgtgcccgct ctccgcctgg aactacattt cgacggtccg gacttcgatc
     1681 ccgtatcgcg gatccatcgg gcagtggcgc gcgacttcca gcgcgtcgtc gaaaccggcg
     1741 acatcaacga cgtagaagcc gccgatcgac tccttgagat cggcatgcgg gccgtcggta
     1801 atcaggaact cgccgtcctg ctggcgaatc gtcttcgccg tcgcgggcga tccgagcgcc
     1861 gcacccgata cgatcgcggt gccgcgctcc gcacaccagg cggcaaactc ctgatgcgcc
     1921 ttccctgtct cgcccattgc ggcgggatcc tgctcgttcc accacggcga agattcgttt
     1981 ccaatgatca gcatgtactt catggatgcg tggcctttcc tggattccct ggaatcggga
     2041 tgtcggtcaa tcgggacgac tggagcgcta gtgctcccag aacgggcgaa cttcgattcc
     2101 ggagtcggaa tccatcgggc agtgtttggc gatctccgcg gcagcggcag catcaggcgc
     2161 gtcgatcaca tagaagccac cgacgacttc cttgagatcg gcgtacggac catcggtcac
     2221 gacgaactcg ccattcttct tccgcaccgt ccgcgcggcg tccggtccga aaagcgcgct
     2281 ccctcccgag tacgcgacgc cgtttgcatc ga
//
LOCUS       DASTMD010000341         3210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_299315, whole genome shotgun sequence.
ACCESSION   DASTMD010000341 DASTMD010000000
VERSION     DASTMD010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299315"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFQ54_04985"
     CDS             <1..259
                     /locus_tag="VFQ54_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164378.1"
                     /translation="ATMPMSRVARAFEVDEPEGFMKVLVDPETEQILGAAILGINGGE
                     IMAMLQIAMMGEIPYTRLRDAVWAHPTLAEGLNNLFFSFRD"
     gene            457..2286
                     /locus_tag="VFQ54_04990"
     CDS             457..2286
                     /locus_tag="VFQ54_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164379.1"
                     /translation="MPESSSPLPYAERRDRFAADRERIQRRWSLIANVRLLGFAVLAI
                     LLWQALFVSGSAVWLVLTAVALVVVVALVIYHARLRRERDHIGRLVTVNGLAIQRAHL
                     DWDDLPLPPESVQARSHAYAYDLNVIGRASLEQRIGTPATQRGWEALRRWLLAPADLD
                     AIGERQVAVSELAVEVDRRQDVEAAAHRPAGTIGDPAPLVDWAEGPAWLRDRAWLLGI
                     AWISPVALVLAIVAQVTGITPWPLWLIPIVVNVAVFQFVGARASELVAKVAPLHQSIE
                     GYGDVFAAIATGEPRAPMLARIRGVLGSGPAGAMTQIRTLVRASSLALPRGSMLYFPF
                     QMAMLWDIHVLASLERWQRVAGPNVRTWIDAAAEWEALAALSVLAYDHPAWTMPDVAP
                     EHDRFAATAMAHPLILPEEAVANPVDVGPRGSLLFVTGSNMSGKSTLLRAVGVNAVLA
                     LAGGPVAATSLSLPPVDVWTCMRVEDSLARGVSFFMAELQRLKAVVDAADAARVRPVL
                     YLLDEILQGTNTAERQIASRQVLRHLSRANAIGAVSSHDLALLEGDQLESIARPVHFA
                     ETFQRGPTGPDMTFDYTLRQGLATSTNALKLMELLGFDLSEPS"
     gene            2397..>3210
                     /locus_tag="VFQ54_04995"
     CDS             2397..>3210
                     /locus_tag="VFQ54_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0164380.1"
                     /translation="MRITDVTVTAVRADAKEKSWLSESVVANPMSIYPDYKARRSSWN
                     AQWGPDLLVRVWTDEGITGIGCSVPAGARSIIEDHYRNLLVGQDPFDIEKLWDQMFRS
                     SLPYGRKGLPIMAISGVDIALWDIVGKALGQPVWKLIGGATKDGIPVYSTGNDVGFFN
                     SLGFRGFKLAMPYGPVDGWDGMKKNVELIEAARETVGPDGEIMLDCYMAWNVDYTLRM
                     SRLIEPYRIRWIEECLPPDDYEGYAELTAKSLVPVATGEHEYTRWGYKELISR"
BASE COUNT          495 a         1024 c         1104 g          587 t
ORIGIN      
        1 cgcgacgatg ccgatgagcc gcgtcgcccg tgccttcgag gtggacgagc cggaggggtt
       61 catgaaggtg ctcgtcgacc ccgagacgga gcagatcctg ggagcggcga ttctcggcat
      121 caacggtggc gagatcatgg cgatgctgca gatcgcgatg atgggcgaga ttccctacac
      181 ccggttgcgg gacgccgtct gggcgcaccc gacgctggcc gagggattga acaacctgtt
      241 cttctcattc cgggattgag cggtgcgtcc acaagatcgt cgacggggcg tgagcccccg
      301 cgaggcggga catagacgtc ccgccgtacg cacgaccgcc gtacgtgggg cgacatcggg
      361 cgagattcgt tgtggtcagg accgggtcgg caccaggccg acccccacgg atatgcgtga
      421 ttgagtgttt cggagggttc catcgttacc tcatccttgc ccgaatcttc ctcgcccctt
      481 ccatatgcgg aacgtcgcga tcggttcgcc gccgatcgtg agcggatcca acgccggtgg
      541 agcctgatcg ccaacgtccg gctcctcggt ttcgcggtgc tcgcgattct cctctggcag
      601 gcgctcttcg taagcgggtc cgccgtctgg ctggtgctca ccgcggttgc gctggtcgtc
      661 gtcgtcgccc tcgtcatcta ccacgcccgg ttgcggcggg aacgcgacca cattggtcgc
      721 ctggtgacgg tcaacgggct ggcgatccag cgtgcccatc tcgattggga tgacctgccg
      781 ctgcctcccg agagcgtgca ggcgcgctcg cacgcgtacg cgtatgacct gaacgtgatc
      841 ggccgggcgt cgctcgaaca gcggatcggg acaccggcga cgcagcgcgg ttgggaggcg
      901 ctgcgcaggt ggctcctcgc tcccgccgac ctcgacgcga tcggtgagcg ccaggttgcc
      961 gtcagcgaac tggccgtgga ggtcgatcga cgacaggatg tcgaggccgc cgcgcatcgc
     1021 ccggccggga cgatcggaga tcccgcgccg ctcgtcgatt gggcggaggg accggcctgg
     1081 ctccgcgatc gcgcctggct cctgggcatc gcctggatct cgccggtcgc actcgtgctg
     1141 gcgatcgtcg cccaggtgac gggcattacc ccgtggccgc tgtggttgat cccgatcgtc
     1201 gtcaatgtcg ccgtcttcca gttcgttggt gcgagggcat cggagctggt ggcgaaggtc
     1261 gcgccactcc accagtcgat cgaggggtat ggagatgtct tcgccgcgat cgcgacagga
     1321 gagccccggg cgccgatgct cgcgcggatc cggggtgttc tcggctcggg gccggctggg
     1381 gcaatgacgc agatccggac gctcgtgcgc gccagctcgc tggcactgcc gcggggttcg
     1441 atgctctatt tcccgttcca gatggcgatg ctgtgggaca ttcacgtgct cgcctcgctg
     1501 gaacgctggc aacgggtggc cggtccgaat gtgcggacgt ggatcgatgc cgcggcggaa
     1561 tgggaagcgc tcgccgcgct ctcggtcctc gcatacgacc atccagcatg gacgatgccg
     1621 gacgtcgcgc cggagcacga tcgcttcgcg gcgacggcga tggcgcaccc gctgatcctc
     1681 ccggaggagg cggtcgcgaa tccggtcgat gtggggccgc gaggatcact cctcttcgtg
     1741 accggctcga acatgtcggg caagagcacg ctgctgcgtg cggtcggcgt gaacgcggtc
     1801 ctcgcgctcg ccgggggacc ggtcgccgcg acgtcgctca gcctgccgcc ggtggatgtc
     1861 tggacctgca tgcgggtcga ggattcgctc gcccgcggcg tctccttctt catggcggag
     1921 ttgcagcgcc tgaaggcggt cgtcgatgcc gctgacgcag cgcgggtgcg tcccgtcctc
     1981 tacctgctcg acgagatcct ccagggcacg aacaccgccg agcggcagat cgcctcgcga
     2041 caggttctcc gtcatctcag ccgcgcgaat gcgatcggcg cggtctccag tcacgatctc
     2101 gcgctgctcg aaggagatca gctggaatcg atcgcacgac ccgtgcactt cgcggagacc
     2161 ttccagcgcg gtccgaccgg tccggacatg accttcgact acacgctgcg gcaggggctg
     2221 gcgacgtcga cgaacgcgct caagctgatg gaacttctcg ggttcgatct ctccgagcca
     2281 tcctgaccgc tggttcgtcg cgccgttgtg ccttcgtgcc tccacgcata tgatgcgccc
     2341 atcgaacgat ccgaaccgct cccgatcgat gggctctctt cgcaaggagt gctggtgtgc
     2401 gcattaccga tgtgaccgtg accgccgtgc gagccgacgc caaggagaaa tcctggcttt
     2461 cggaatccgt cgtcgccaac ccgatgtcga tctatcccga ttacaaggcg cggcgatcct
     2521 cctggaacgc gcagtgggga cccgatctcc tcgttcgcgt ctggacggat gaggggatca
     2581 ccgggatcgg ctgctcggtg ccggctggcg cacgcagcat catcgaggat cattaccgga
     2641 acctccttgt cgggcaggat cctttcgata tcgagaagct gtgggaccag atgttccgct
     2701 cctcgctccc gtatggacgc aaggggctgc cgatcatggc gatcagcggc gtcgatatcg
     2761 cgctgtggga tatcgtcggc aaggcgctcg ggcaaccggt ctggaagctg atcggcgggg
     2821 cgacgaagga cgggattccc gtctactcga cgggcaacga cgtcggcttc ttcaattcgc
     2881 tcggctttcg cggcttcaag ctggcgatgc cgtacggccc ggtcgatggc tgggacggca
     2941 tgaagaagaa cgtcgagctg atcgaggcag ccagggagac cgtggggccg gatggcgaga
     3001 tcatgctcga ctgctacatg gcgtggaacg tcgactatac gctccggatg tcgcgcctga
     3061 tcgagcccta ccggatccgg tggatcgagg aatgccttcc tccggacgac tacgagggat
     3121 acgccgagtt gacggcgaag tcgctggtgc cggtcgccac gggggagcac gagtacacgc
     3181 ggtggggata caaggagctg atctcccgcc
//
LOCUS       DASTMD010000342         6124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2709886, whole genome shotgun sequence.
ACCESSION   DASTMD010000342 DASTMD010000000
VERSION     DASTMD010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2709886"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFQ54_05000"
     CDS             complement(<1..197)
                     /locus_tag="VFQ54_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164381.1"
                     /translation="MEFVNATPAPELTLSIVEQLMEIVELQAGQATESHDHWAYSWWL
                     ATGREYGQDLAPSLSRAVAGLS"
     gene            complement(539..1372)
                     /locus_tag="VFQ54_05005"
     CDS             complement(539..1372)
                     /locus_tag="VFQ54_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164382.1"
                     /translation="MTRNRSRRRGRRPGQGDGDLPVDESQSQSQGSAGATAAAASASD
                     GGDQPGSGSRSGGQRPQGPQAAGDDANRGGQPGPQGEKSGRRRRRGQGGGSAASAERP
                     EQPQAERPSRPPAQERNARPERSRSGPGQGQARGNQPPRDDQSADGRGQGAGRQRRRN
                     SRRRPAQPPMPETVLKETAPIPVPARPMVMRKLDELGVGESITLGCPMLTRTRLALHT
                     NEGLRAGRCSLGWALHGEEEVRFCLHTPNSLDCWKAHPERVEVIQEEIEKEALAASAA
                     D"
     gene            complement(1353..1949)
                     /locus_tag="VFQ54_05010"
     CDS             complement(1353..1949)
                     /locus_tag="VFQ54_05010"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEU0164383.1"
                     /translation="MNDQPTSPLGGDGIDKIEPGQSESVVFAGQGSKVEASPETPVKA
                     DADPDVLAAFWEAVKRLPAYGRLSYAIARDPKVPNQAKGLLAAGGIYLVSPIDLIPGV
                     IPVVGQLDDLYVLLTALQYAVKNCPAEIIAPHLTSAGVTQKQIEDDLASIRHLVRFAV
                     VKGWKLGSRAIARAGGNVTARFRKAAQREKANDDQKPI"
     gene            complement(1969..2634)
                     /locus_tag="VFQ54_05015"
     CDS             complement(1969..2634)
                     /locus_tag="VFQ54_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164384.1"
                     /translation="MIDILVVDHVLGEALGVASGEIAHLGLDLPEMTGDLEWREAAVE
                     EPQDITGERGSDWLAGFTAQRLVQGADRLLVMILDGPDTEAQFFRYFLVGEPIGLQAK
                     YLALPASKARATIGSCRDHHVDAPSGTPPSEKQNADLTEAGLSETGNDSDIVAKNWPV
                     VFRINGGVFRLLYVDRNVGSFHVTSGRWVAQLPGHVVCMSRRPAGGIWMMKGFTFVHL
                     AWR"
     gene            complement(2653..3573)
                     /locus_tag="VFQ54_05020"
     CDS             complement(2653..3573)
                     /locus_tag="VFQ54_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEU0164385.1"
                     /translation="MSVMRRTVLAVSDNRVVSGFFKSNRLARGLVDRFVAGESLEEGL
                     AACRDLERHGIVTTLDQLGENVATVEEATAAAASFQSILRAMSTVGLETNISVKLTML
                     GLDLGRSVAEENLVSVLDVAKEVGGFVRIDMEGSLYTAITMEIFESLFARYPENVGIV
                     TQTYLRRAEQDVKRAIELGARVRLVKGAYAEPESIAFTTRAENDQNYLHLMRLLLEGG
                     NFPAIATHDPALIAAAQHHAKGREISPNRFEFQMLYGVRREEQQRLVDAGYRMRVYVP
                     FGTEWYPYFTRRIAERPANALFVLRAMRGK"
     gene            3808..5163
                     /locus_tag="VFQ54_05025"
     CDS             3808..5163
                     /locus_tag="VFQ54_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU0164386.1"
                     /translation="MQYADMSIDGDVTGPAVTVMRDGLYDLIPLSSAELAIMATPSFL
                     RLDRIQQLGFVSRVWPGAKHTRYEHSVGVMHLTRLAVQRLARREEGRWITPEDARTVI
                     AAALLHDIGHYPFSHAIEELGYPVRSHEEVGRAIIERGEIAEILEREWAVSPGRVAAL
                     IHAGRDALTGADRVLSGLLSGTLDMDKLDYLPRDARACSVPYGGVDSVRLLNALTIAN
                     VDEAGWTPEPPRLVIEEKGISPLHSLINARQEMFDNVYWHHTNRACMIMLLRAVQEAL
                     VTGNITSTNLTDHDDATLLAFMRSPEMPEPTRNLADALANRRIHKRALEVSARAQELY
                     GRLGNLWFDPVARRSVEIDLCRSLAKLLGEPVPLTAILIDIPKPERWKTDVWVQFERP
                     PVGFQRLMHWRDVVGLGDDDFKRYEEHRRLIRIVTEERYRDIVATHWEQLIYPLLGGA
                     F"
     gene            5742..>6124
                     /locus_tag="VFQ54_05030"
     CDS             5742..>6124
                     /locus_tag="VFQ54_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164387.1"
                     /translation="MKRSHQIARPNRRWMLFALAISILVAITPIASRFVTAPALAQSA
                     TMATPDADQVPTDAPVATGAPLPTETTVPTDVPTETPSPTPSPTDTPTPTNTPEPTVT
                     PTSIATVEPSSTAGPTNAPTSTSAPT"
BASE COUNT         1066 a         2126 c         1860 g         1072 t
ORIGIN      
        1 gagagcccgg cgacggccct cgacagactc ggagccaggt cctggccgta ttcccgaccg
       61 gtggcgagcc accaggagta cgcccagtgg tcgtgggact cggtggcctg gccagcctgg
      121 agttcgacga tctccatcaa ttgctcaacg atggagaggg tcaactctgg cgcgggagtc
      181 gcgttgacga actccatcac actccataca tgcgtagtcg tggctcccac accgaggcgg
      241 gccggggtgg gatagccgcg cccacgcatg tgcgcgacca cgctttgggt tcgcaacacc
      301 tcgtccagct ggttggggcg tgtccggggc catgctttga gtattgcgtc tgcccttcca
      361 gccaaccgga cgcgtatggc gcccacatcg aagcctccgg gcaggcgatc gagaagcgtg
      421 acctcggccc cgaccacgct ggacacgtcg tccaggacct cggctggcaa ctcgtcgggg
      481 tgaatttcct ggttcatttg gagttcatct cttgccgaga acgggaacgt cccacgctct
      541 aatcggctgc gctggcggcc agtgcttcct tctcgatctc ctcctggatg acttccaccc
      601 gttccgggtg ggccttccag caatcgagcg agttgggagt gtggaggcag aatctcacct
      661 cttcctcacc gtgcaacgcc catccaagcg agcagcgtcc agcgcgcaac ccctcgttcg
      721 tatggagcgc gaggcgggtc cgcgtcagca tcgggcagcc cagggtgatc gattcaccca
      781 cgccaagctc gtcgagcttt cgcatgacca tcggtcgcgc cggcaccggg atcggcgccg
      841 tctccttgag cacggtctcc ggcatcgggg gctgcgctgg tcggcgccgt gaatttcgcc
      901 ggcgctgacg accggcgccc tggccgcgtc catcggcgga ctgatcgtcg cgcggaggct
      961 ggtttccccg cgcctgaccc tgcccgggac cggatcgcga ccgctcgggg cgggcattgc
     1021 gctcctgtgc cggtggcctc gacggacgct ccgcctgggg ctgctccgga cgttcggccg
     1081 aagcggcgct cccgccgccc tgaccgcgtc gccggcggcg gccggatttc tcgccttgcg
     1141 gtcctggctg accaccccgg ttggcgtcat cgcccgccgc ctgcggtccc tggggacgct
     1201 gtccgccgga ccgggacccc gatccaggct ggtccccacc atcggaagca gaagcggcag
     1261 cagccgtcgc cccggcgcta ccctgtgatt gcgattgcga ctcgtccacc ggcaggtcac
     1321 cgtcaccctg gcccggtcga cggccccgtc gcctagatcg gtttctggtc atcgttcgcc
     1381 ttctcccgct gggcagcctt ccggaaccgg gcggtcacat tccccccggc cctcgcgatc
     1441 gcccggctgc caagcttcca gcccttgacg accgcaaacc gcacgagatg ccggatcgac
     1501 gcgagatcat cctcgatctg cttctgggtc acacccgcgc tcgtgagatg cggcgcgatg
     1561 atctcggcag gacaattctt cacggcatac tgaagcgcgg tcagcaggac gtagagatcg
     1621 tcgagttgcc cgacgacggg gatgacgccg ggaatcaggt cgatcggtga gaccaggtag
     1681 atcccacccg ccgcgagcaa gcccttcgcc tggttgggga ccttgggatc ccgtgcgatg
     1741 gcgtaggaga gccgcccgta ggccgggagc cgcttgacgg cctcccagaa cgccgcgaga
     1801 acatccggat cggcgtccgc cttgacggga gtctcaggac tggcttcgac cttgctcccc
     1861 tggcccgcga agaccacgga ctccgactgc ccgggctcaa tcttgtctat cccatctcca
     1921 ccgaggggag acgttggttg gtcgttcatc tctcgctcct tgatgtcgtc aacgccacgc
     1981 caggtggacg aaggtgaaac ccttcatcat ccagatcccg cctgccggac gacgcgacat
     2041 gcaaacgacg tgacccggca attgtgccac ccaacgtcca ctcgtgacgt gaaaagaacc
     2101 cacatttcgg tcgacatata acaatctgaa cactccgccg ttgatacgga agacgacggg
     2161 ccagtttttc gctacaatgt cgctatcgtt tccagtctca gacagcccgg cctcggtgag
     2221 gtccgcgttt tgcttttcgc tcggtggagt cccggagggg gcatcgacgt ggtgatcacg
     2281 acaacttccg atcgtagcac gagccttact cgccggcagc gcgaggtact tcgcctgcag
     2341 gcctatgggc tcacctacca ggaaatatcg gaaaaactgg gcctcagtat ctggaccgtc
     2401 aagaatcatc acaagcaggc gatccgcgcc ctgcaccagg cgttgggcgg taaatcccgc
     2461 cagccaatca gaacctcgct cgcctgttat atcctggggc tcttcgacgg cggcctcacg
     2521 ccactcgaga tcgcccgtca tctccggcag gtccagcccc aggtgcgcga tctcgccact
     2581 ggcgacaccg agagcctcgc caagcacgtg atcgacgacg aggatgtcga tcacgcggcc
     2641 tagcggtccc gatcacttcc cccgcatcgc gcgcagtacg aagagcgcgt tcgccggtcg
     2701 ctccgcgatg cgccgcgtga agtagggata ccactccgtg ccgaagggca cgtagacccg
     2761 catccgatac ccggcatcga cgaggcgctg ctgctcttcg cgccgcaccc cgtagagcat
     2821 ctggaactcg aagcggttcg gcgaaatctc gcggcccttc gcgtgatgct gggctgccgc
     2881 gatcagcgcc ggatcgtgag tcgcaatcgc cgggaagttc ccgccctcga gcagcagacg
     2941 catcaggtgc aggtagttct ggtcgttctc cgcgcgcgtc gtgaaggcga tcgactccgg
     3001 ctccgcgtac gcacctttca ccagacgcac gcgcgccccc agctcgatcg cgcgcttcac
     3061 gtcctgctcg gcgcgtcgca ggtaggtctg ggtcacgatc ccgacgtttt ccggataccg
     3121 ggcgaagagg gattcgaaga tctccatcgt gatcgcggtg tacagcgatc cctccatgtc
     3181 gatccgcacg aagccaccga cttccttcgc cacgtcgagg acggagacca ggttctcctc
     3241 ggcgacggag cgaccgagat cgaggccgag catcgtcagc ttgaccgaga tgttggtctc
     3301 gagcccgacc gtgctcatcg cacggaggat gctctggaag gacgccgccg ccgcggtcgc
     3361 ctcctcgacg gtcgcgacgt tctcgcccag ctgatcgagt gtcgtcacga tgccgtggcg
     3421 ctcgagatcg cggcatgcgg cgagcccctc ctccagggat tcgcccgcca cgaaccggtc
     3481 gacgagaccg cgcgccagcc ggttcgactt gaagaatcca ctgacgacgc gattgtccga
     3541 aaccgccagc accgtccgcc gcatcacact catcgcgcgt aatctccgtc acagatcgtc
     3601 gttgtcatgc tggttgtcgc gcgccactac gcgaatgttc gcccggatac acgcgagccc
     3661 gggtgccccg gtcgtcaatc gctgaagtat cccacgaact cccgggcatc acagcgtgat
     3721 tgcggggccg ccgtgacagg tacgagggcc ggcatcgtat cctgcatcca aacatcgccg
     3781 atcgggattg aaggagcgcc gggactcgtg cagtacgcag acatgtcgat cgatggagac
     3841 gtcactggtc ccgccgtgac cgtgatgcgg gacggtctct acgacctcat cccgctgagc
     3901 agcgccgagc tcgcgatcat ggcgacgccg tccttcctgc gcctcgaccg catccagcag
     3961 ctcggcttcg tctcgcgcgt ctggccgggc gcaaagcaca cgcgctacga gcattcggtc
     4021 ggcgtcatgc acctgacccg gctcgccgtg caacgccttg cgcgtcgcga ggagggacgc
     4081 tggatcaccc cggaagatgc ccgcaccgtc atcgccgccg ccctcctgca cgacatcggt
     4141 cactacccgt tctcccacgc gatcgaggag ctcggctacc cggttcgctc ccatgaagag
     4201 gtcgggcgcg cgatcatcga acgtggcgag atcgcagaga tcctcgagcg ggagtgggcc
     4261 gtcagcccgg gccgggtcgc cgccctgatc cacgccggac gcgacgccct gaccggcgcc
     4321 gaccgtgtgc tgagcggtct cctctccgga acgctcgaca tggacaagct cgactatctc
     4381 ccccgcgacg cgagagcctg cagcgtgcca tacggcggcg tcgattcggt ccgcctgctc
     4441 aacgccctga cgatcgccaa cgtcgatgag gctggctgga cacccgagcc gccccggctt
     4501 gtgatcgagg agaagggaat cagtcccctt cattccctga tcaacgcgcg gcaggagatg
     4561 ttcgacaacg tctactggca tcacaccaac cgcgcctgca tgatcatgct ccttcgcgcc
     4621 gtccaggagg cgcttgtcac cggcaacatc acgtcgacca acctcaccga tcacgacgac
     4681 gcgacgctgc tcgccttcat gcgttcgccg gagatgccgg agccgacgcg caacctggcc
     4741 gacgccctgg cgaatcgccg aattcacaag cgcgccctcg aggtcagcgc ccgcgcccag
     4801 gagctgtacg gacgactcgg caatctctgg ttcgatccgg tcgcccggcg ttcggtcgaa
     4861 atcgatctct gccgctcgct ggcgaagctg ctcggcgaac cagtgccgtt gacggcgatc
     4921 ctgatcgaca tcccgaaacc ggagcgatgg aagaccgacg tctgggtcca gttcgagcgg
     4981 ccgccggtcg ggtttcagcg cctcatgcac tggcgcgacg tggtcggcct tggtgacgac
     5041 gacttcaagc gctacgagga gcaccgccgg ttgatccgga tcgtgaccga ggagcgctac
     5101 cgcgacatcg tcgcgaccca ctgggagcag ttgatctacc cattgctcgg cggcgcattc
     5161 tgaggcgaaa tccgggaacg tcgacacgtc tggtgtcgct ggggttggta gcgcggggtt
     5221 ttgtaccccg ccgcaacgcg gactccggag aaacgcgccc tgccccgaga cggttaccgt
     5281 ctcgccctcc gccgcaggcg gacgccgacg ggaatctgcg gtgcacgacg ttcccggggc
     5341 gaaggagagg cgggacaggc cgtccggacc tgcggtccgg cagcggcggt cccgccctac
     5401 gaacgcacgg agcgccgcga ctgaaccggc cgccgcctcg cagcgacgcc agccggaggt
     5461 cctgcatctt gcggtacttg tatcgagatc aatcgacaaa cgactgacgt accggttata
     5521 tcggcccccc ctaacctgcg cagaattaca agtcgatccg atcaatagtg tccatgagtc
     5581 ctgtttactg gcatcgccca cttatgccat gtagctttat tgctgtttgg ttcgtgttgc
     5641 tctatgcgca tattttgcaa atacgtgact tatcaaccat caatcacctg aagccaatcg
     5701 cccaacgtgt ttgcatatga tggagtggac gggatatcga cttgaagcgt tcgcatcaga
     5761 tcgctcgccc aaaccgccgg tggatgctct tcgccctggc gatttcgatt ctggtggcaa
     5821 tcacgccgat cgcatcgcga ttcgtgaccg cgcccgcgct tgcgcaatcc gcgacgatgg
     5881 caacgccgga tgcagatcag gtccccacag acgccccggt tgcaaccggg gcgccgcttc
     5941 cgacggagac gaccgttccg accgacgtac caaccgagac accatctccc acgccgtcac
     6001 cgacggatac accaacgcca accaacacgc cggaaccgac ggtcacaccg acgtcgatcg
     6061 ccaccgtcga accatcttcg acggcggggc ccacgaacgc accgacgtca acgagcgcgc
     6121 caac
//
LOCUS       DASTMD010000343         2783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2709913, whole genome shotgun sequence.
ACCESSION   DASTMD010000343 DASTMD010000000
VERSION     DASTMD010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2709913"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFQ54_05035"
     CDS             complement(<1..740)
                     /locus_tag="VFQ54_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0164388.1"
                     /translation="MESTVESSIEKASLIVGMPKLVCSTGAISRFTDLNDHQGFIDRS
                     LQLHADAIEVMIFDRWYERLDEIAADFRAAGIRVVATHAEKNLGPHLAQDDPEFVAQT
                     LARFSENCRFTHLLGGDRTVLHLWGLPESDKVLDRMLAMLPELVDRAAEHHLYLGVES
                     IPCVVADPLTNLQRVLEAEPRSKVTLDTEFLAMHGQVDAVLSADWLWDRDAVVHLHVK
                     DFIDPSNIDPSKRTYLHPGEGEIDFAGL"
     gene            937..1221
                     /locus_tag="VFQ54_05040"
     CDS             937..1221
                     /locus_tag="VFQ54_05040"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEU0164389.1"
                     /translation="MARMVQCVKLGRSLPGLEKPPFPGELGQRIYENISAEGYALWMP
                     HMTILINHYALNPADPETRAMLRKEMNEFFFGEGAFKTADLNPDDVTSGD"
     gene            complement(1313..1594)
                     /locus_tag="VFQ54_05045"
     CDS             complement(1313..1594)
                     /locus_tag="VFQ54_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164390.1"
                     /translation="MAFDLSPADTSVLLFHSVDAAEDAGAAMDAVNTWLGRDRSGGAY
                     ANLRIRDVTVTPDGRGGVFTTIVCTLGKLSTASVSNAGASRDQFVEGNE"
     gene            1850..>2783
                     /gene="purD"
                     /locus_tag="VFQ54_05050"
     CDS             1850..>2783
                     /gene="purD"
                     /locus_tag="VFQ54_05050"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU0164391.1"
                     /translation="MRVLVVGGGGREHAIVWKLAQSERVREILCAPGNPGIEQLATCI
                     NVPTHHIDGIVALAKVRQVDLVVVGPEDPLAAGLADRLTEAGIAVAGPSAAASQIESS
                     KSWAKGLMEEGGIPTARSITVTDREAGYRAAAELANPDGGVVIKADGLAAGKGVVVAT
                     SLKEAERAIESFMSLRALGDAGKTVVVEELLTGMELSVLSITDGKTIYPLIPSCDYKR
                     ANDNDDGPNTGGMGAYTRPASVDAALMTHIQATILEPAIRAMAARDCPMKGILYAGLI
                     LTPDGPKVIEFNARFGDPETQVVLPTLDGDLGLLM"
BASE COUNT          533 a          903 c          881 g          466 t
ORIGIN      
        1 aagagcccgg cgaaatcgat ctcgccttca ccagggtgga ggtaggtccg cttcgaggga
       61 tcgatgttgc tgggatcgat gaaatccttg acatggagat ggacgaccgc atcacgatcc
      121 cagagccagt ccgcggagag aacggcatcc acctgcccgt gcatggcgag gaactcggta
      181 tcgagggtca ccttcgaccg cggctccgcc tccagcacgc gctggagatt cgtgagcgga
      241 tcggcaacga cgcagggaat cgactcgacg ccgagataga ggtgatgctc cgccgcacga
      301 tcgacgagct ccgggagcat cgccagcatc cggtcgagca ccttgtccga ttccggcagt
      361 ccccagaggt gaaggacggt gcgatcgcca ccgaggagat gcgtgaagcg gcagttctcc
      421 gagaagcgtg cgagggtctg ggcgacgaac tcgggatcgt cctgcgcgag atgcgggccg
      481 aggttcttct cggcgtgcgt cgcgacgacg cggatcccgg cggcgcggaa gtcggcggcg
      541 atctcgtcga ggcgctcgta ccagcgatcg aagatcatca cctcgatcgc gtcggcgtga
      601 agctggaggc tgcggtcgat gaatccctgg tgatcgttga gatccgtgaa gcgggagatc
      661 gccccggtcg aacacacgag cttcggcatg ccgacgatca acgacgcctt ctcaatcgaa
      721 ctctcgaccg tgctttccat gctgtcccga ctccttcgct ggccaacgat ccgtgaccgt
      781 gaccgcgtga tggctttggt acactctgcg acaggcgatc ggccgggatt tccgggatca
      841 ccgcgattga ctcggcgact ccgcgcagcg agcgcgaacc gccgcctgtc atgttcggga
      901 catcatcgca cccaatctca acggagagta ttcgtcatgg ctcgcatggt ccaatgcgtg
      961 aagcttggcc gctcactgcc cggcctcgag aagccgccct tccccggcga gctcggtcag
     1021 cgcatctacg agaacatctc ggccgaggga tacgccctct ggatgcccca tatgaccatc
     1081 ctcatcaatc actatgcgct caacccggcc gatccggaga ctcgcgccat gcttcgcaag
     1141 gagatgaacg agttcttctt cggcgagggc gcgttcaaga ccgcggatct caacccggac
     1201 gatgtcacat ccggcgattg agcgcagcgt cgacattccc acgggcaaca cgcaacaagg
     1261 gcgcttccgg catgatccgg aagcgccctt gaatgattcc cgcagcggag gattactcgt
     1321 tgccctcgac gaactggtcg cgagaagcac ccgcattgct gaccgaggcc gtgctcagct
     1381 tgccgagcgt gcagacgatc gtcgtgaaga cgccgccgcg gccatccggc gtaaccgtga
     1441 catcgcggat ccgcaggttg gcataagcgc cgccgctgcg atcgcgaccg agccaggtgt
     1501 tgacggcatc catcgcggcg ccggcatctt cggccgcatc gaccgagtga aacagtaaga
     1561 cactggtatc ggccggggaa agatcgaacg ccatcgtggg gttcctttca tcagttcgac
     1621 agccggcctg aatttggtcg ttcgacgcat cacactgcga ggctgtgttc aatccacagg
     1681 gtcagcccga accaccagat ccgatcgcct tccctaccgc ggaagagtat acgaggttcc
     1741 tcgcgcttgg gggcgatcac cattgggtat catgtcccca ttctgttgag ggcacgggag
     1801 aatctcctgg cggcgccctt ctgaaccaag ggagaaggtt gcggtgagca tgcgggtact
     1861 tgtcgtcggc ggcggtggac gcgaacacgc gatcgtctgg aagctcgcgc agagcgagcg
     1921 ggtccgcgag atcctctgcg ctccaggcaa cccgggaatc gagcagctcg ccacctgcat
     1981 caacgttccc acccatcaca tcgacgggat cgtcgccctc gccaaggtcc gccaggtcga
     2041 tctcgtcgtc gtcggaccgg aagatccact ggccgccggt ctcgccgatc gcctcaccga
     2101 ggctggtatc gccgtcgccg gaccgagcgc ggccgcgagc cagatcgaga gcagcaaatc
     2161 ctgggcgaag ggattgatgg aggaaggcgg gattccgacc gcccgctcga tcaccgtcac
     2221 cgatcgcgaa gccggctacc gcgccgccgc cgagctcgcg aatcccgacg gaggcgtcgt
     2281 gatcaaggcg gacggtctcg ccgcaggcaa gggcgtcgtg gtcgcaacga gcctcaagga
     2341 ggccgaacgc gccatcgaat cgttcatgtc gctccgggcg ctcggcgacg ccggaaagac
     2401 ggtcgtcgtc gaggaactgc tcaccgggat ggagctctcc gtcctctcga tcaccgacgg
     2461 caagacgatc tatcccctca tcccctcctg cgactacaag cgcgcgaatg acaacgacga
     2521 cggcccgaac accggcggca tgggcgccta cacgcgacca gccagcgtcg acgcggcgtt
     2581 gatgacccac atccaggcaa cgatcctcga gccggcgatc cgggcgatgg cggcgcgcga
     2641 ctgtccgatg aagggcatcc tctacgcggg gctgatcctg acaccggacg ggccgaaggt
     2701 gatcgagttc aacgctcgct tcggcgaccc cgagacgcag gtcgtccttc cgacgctcga
     2761 cggggatctc ggtctcctga tgg
//
LOCUS       DASTMD010000344         4073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1192617, whole genome shotgun sequence.
ACCESSION   DASTMD010000344 DASTMD010000000
VERSION     DASTMD010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192617"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..892)
                     /locus_tag="VFQ54_05055"
     CDS             complement(137..892)
                     /locus_tag="VFQ54_05055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0164392.1"
                     /translation="MRFGSLSLVRRGLTAVAAGTLFVSSIAGLGATSALAQDATASPG
                     AAGTPAPTVVTNVDTSGCWSAAPTYTSTGASLYPQWTTAPNMVIDPNGTYTATITTNL
                     GVVTIQLYASKAPTTVNNFVCLAGHGYYDKTVFHRILAGFVVQGGDPTGSGSGGPGYQ
                     FKDELPTDLNYTAGVVAMANAGANTNGSQFFICLDDLSSRLTKSYSIFGTVSGGMDVV
                     NALGAVPVTTGPSGESSSPTAPAGIISVAITKA"
     gene            complement(977..2542)
                     /locus_tag="VFQ54_05060"
     CDS             complement(977..2542)
                     /locus_tag="VFQ54_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164393.1"
                     /translation="MFLRAGAATAAAAVVTTFPRHGRQQTETATSSTSQSFGGDPVVA
                     LAHIPDGNDLVTGATAESIWLGGLCHDTLFMPDTNGDPAPGIAVAPFFNREGFELDFS
                     VREGVIFPDGSLLTAEDVVASLERIRGMGPNSAQGWRLEHIDAIEIPEPGTVRLTLTQ
                     PDYSLLGCLGSSTIPVLPRDIASDLGTYGLPNLPPGTGPFVPGSIPDAETYEFGRNGS
                     FWQIGRPRLDGLTVTSVTEDTQRTISLVTGSVDIVIDAPLLDIKSLKQDPNITLVGGP
                     SVRACVVILNVVEGGMADRRVRKLLSEAINRSALVQAATGGEGSPEQTLFPKATWPGL
                     DQPPPDTDWDKTRKDMMALGYTAGLPLRLITSEQDGSSANAAVLLQEQLGNAGFSVTL
                     DLLDAAALERTLTTRQYDLYIMNTPGWTDPHELVRPLLMSDGPGNLMGYRSARVDSLI
                     RQAVMVETQDDRATIYQQLQTVLLDEVPLIVLFFPNYYDAMSARVKDYGWYPPVHAYG
                     LRTAWMEPPTPSS"
     gene            complement(2604..3809)
                     /locus_tag="VFQ54_05065"
     CDS             complement(2604..3809)
                     /locus_tag="VFQ54_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0164394.1"
                     /translation="MSTEISTSEFPTSVDQILEIEKSLQIPLYAKRDIALVRGEGSYL
                     FDSDGNRYLDAMSNYGVAVLGHAHPRYAQALSEQLRTLTTAHQSFGNDVRAALLLELN
                     TIAPEGVRRSFLSNSGAEAIEAALKFARASTGKHSIVALRRGYHGRTLGALSATADKK
                     YREPFEPLPIPSTHVAFNDADAVAAAIDDDTAAIIFEPIQGEGGIHPGDVEFLAAIRR
                     LATERGTVLIADEIQSGFRTGAPFASVAAGIVPDILVTAKALANGFPIGLTLVTEEIS
                     ASLPAGAHGSTFGGNPLAMRAAYETLRILREEDLYSRATELGARIIEGIESLKSPKVR
                     AVRGRGFMIGMELKAKATPVLRGLQDKGVLALPAGNLVIRFLPPMNWEQTQVDELLAA
                     LEVVLAASE"
     gene            complement(3881..>4073)
                     /locus_tag="VFQ54_05070"
     CDS             complement(3881..>4073)
                     /locus_tag="VFQ54_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164395.1"
                     /translation="MRPDVGDPELAELVRMFEPPDGELDVLTYDGAEPATRWANELAD
                     ALLAAEEFSSDGGLAFRRC"
BASE COUNT          704 a         1292 c         1375 g          702 t
ORIGIN      
        1 ccttgcgaga tggatatcac gtgtatcggt cgttcgtagg gcgggaccgc cgcgccggac
       61 cgcagtccgg acggcctgtc ccgccggaag tcacccgagg atcgtcttcc gtgcagccac
      121 ggttccggtc ctcacgttac gccttcgtga tcgccaccga gatgatcccg gccggggccg
      181 tcggcgagga gctctcacca ctcggacccg tcgtcaccgg caccgcgccg agcgcgttga
      241 cgacatccat gccgccggac accgtgccga agatcgagta gctcttggtg agccggctgc
      301 tgaggtcgtc gaggcagatg aagaactggc tgccgttcgt gttcgcgccg gcattcgcca
      361 tcgcgaccac gcccgccgtg tagttgagat cggtcgggag ctcgtccttg aactggtacc
      421 ccggtccgcc cgatcccgat ccggtcggat cgccgccctg aacgacaaag ccggcgagga
      481 tgcgatggaa gaccgtctta tcgtagtagc catgaccggc gaggcagacg aagttgttga
      541 ccgtcgtcgg cgccttgctc gcatagagct ggatcgtcac gacgccgaga ttcgtcgtga
      601 tcgtcgcggt gtaggtgccg ttgggatcga tcaccatgtt cggcgcggtc gtccactgcg
      661 gatacagtga agcccccgtc gaggtatagg tcggcgctgc actccagcaa ccgctggtat
      721 cgacgttcgt gacgacggtc ggcgccgggg taccggccgc acccggactc gccgtggcgt
      781 cctgcgcgag cgcggaggtc gcaccgagac cagcgatgct ggagacgaag agggtaccgg
      841 ccgcgacggc ggtcaggccg cgacgaacga gggagagtga accgaagcgc acgagcatgc
      901 tccttgaatc gggttggggt tcaacgctga cgcggaggtc ggctccgcgt cgaccatgga
      961 gaataatgcc acgttctcag gacgagggcg tgggtggttc catccaggcg gtgcgcaatc
     1021 cgtatgcgtg cacgggagga taccatccgt agtccttcac ccgggcggac atcgcatcgt
     1081 agtagttcgg aaagaagagg acgatcagcg ggacttcgtc gagcaggacc gtctggagtt
     1141 gctggtagat cgtcgcccgg tcatcctgcg tctccaccat gaccgcctgc cggatcagcg
     1201 aatcgacgcg cgcgctccgg tagcccatca ggttgcccgg tccgtcgctc atcagcagcg
     1261 gtcgcacgag ttcgtgcgga tccgtccagc ccggggtgtt catgatatag agatcgtact
     1321 ggcgcgtggt gagcgtgcgc tcgagggcgg ccgcgtcgag cagatcgagc gtcaccgaga
     1381 atccggcatt gccgagctgt tcctgcagga ggaccgcggc attcgcggac gatccgtcct
     1441 gttcgctcgt gatcagccgg agcgggagac cggccgtgta accgagcgcc atcatgtcct
     1501 tccgcgtctt gtcccaatcg gtgtccggag gaggctgatc aagccccggc caggttgcct
     1561 tcgggaagag cgtctgttcg ggcgatccct cgcccccggt cgcggcctgc accagcgccg
     1621 agcgattgat cgcctccgag agcagcttcc ggacacgccg gtccgccatc ccgccctcga
     1681 cgacgttgag gatcacgacg caggcgcgaa cgcttggccc accgacgagc gtgatgttcg
     1741 gatcctgctt gaggctcttg atatcgagga gcggcgcgtc gatcacgatg tcgaccgagc
     1801 cggtaacgag cgagatggtg cgctgtgtat cctcggtcac gcttgtcacc gtcagaccat
     1861 cgagtcgcgg tcgcccgatc tgccagaagc tcccgttgcg accgaactca taggtctcgg
     1921 cgtcggggat cgagccaggg acgaacggac cggtgcccgg aggaagattc ggcaggccgt
     1981 acgtaccgag atcggatgcg atgtcccgcg gaagaacggg aatcgtcgac gaaccgaggc
     2041 agccgagaag tgaatagtca ggctgagtca gcgtgaggcg gacggtgccc ggctcgggga
     2101 tctcgatcgc gtcgatatgc tcgagccgcc atccctgcgc ggaattcggc cccatccctc
     2161 gtatccgctc caggctcgcg acgacgtctt ccgcagtgag tagcgagccg tccggaaaga
     2221 tcacgccctc ccggacactg aaatcgagct cgaatccctc gcgattgaag aacggcgcga
     2281 cggcgatgcc cggagcggga tcgccgttgg tatccggcat gaagagcgtg tcgtggcaca
     2341 ggccgccaag ccagatactc tcggccgtcg cgccggtgac gaggtcgttg ccatccggaa
     2401 tatgggcgag cgccaccacg ggatcgccgc caaaactctg ggacgtcgag gatgtggccg
     2461 tctccgtctg ctgccgaccg tgacgaggaa acgtcgtcac gacggccgcg gccgccgtcg
     2521 ccgctccggc gcgaaggaac atgcgacggt tcagccagtg accggatcgc gaaggatggg
     2581 gcgcgggccg gtcccgccgg gaattactca ctggccgcga gtacgacttc gagcgccgcg
     2641 aggagttcgt cgacctgggt ctgttcccag ttcatcggcg gcaggaagcg aatcacgaga
     2701 ttgcccgcgg gaagggccag gacgcccttg tcctgcagac cgcgaagcac cggtgtcgcc
     2761 ttcgccttca actccatgcc gatcatgaag ccgcgaccgc ggacagcgcg aaccttcggg
     2821 ctcttcagcg actcgatgcc ctcgatgatc ctggcgccga gctcggtcgc acgactgtag
     2881 aggtcctcct cgcggaggat tcgcagcgtc tcgtaggcgg cgcgcatcgc gagcgggttg
     2941 ccgccgaagg tggagccgtg cgcgccagcg ggcagcgagg cactgatctc ctcggtcacc
     3001 agcgtcaggc cgatcgggaa gccattggcc agggccttcg ccgtgacgag gatgtcggga
     3061 acgatcccgg cggcgacact ggcgaacgga gcgccggtcc ggaagccgga ctggatctcg
     3121 tcggcgatga ggaccgtgcc gcgctcggtc gcgagtcgcc ggatcgcggc aaggaactcg
     3181 acgtcgcccg ggtgaattcc accctcaccc tggatcggct cgaagatgat cgcggccgtg
     3241 tcgtcatcga tcgccgcggc gacggcgtcc gcgtcgttga atgcaacgtg cgtcgacgga
     3301 atcggcagcg gctcgaacgg ctcgcggtac ttcttgtccg ccgtcgcgga gagagcgcca
     3361 agcgtccgtc cgtgatagcc gcggcgcaac gcgacgatgg aatgcttgcc ggtcgaggcg
     3421 cgcgcgaact tcagcgcggc ctcgatcgcc tcggcgccgg agttgctcag gaacgagcgg
     3481 cgaacgcctt ccggcgcgat cgtgttgagc tcgaggagaa gcgcggcacg aacgtcgttg
     3541 ccgaagctct ggtgcgccgt cgtcagcgtc cgcaactgct cggatagcgc ctgcgcgtat
     3601 cgcgggtgcg cgtgaccgag cacggcgacg ccgtagttgc tcatcgcatc gaggtagcga
     3661 ttgccatcgc tgtcgaagag gtacgatccc tcgccgcgga cgagcgcgat gtcgcgcttc
     3721 gcatagagcg gaatctgcag cgacttctcg atctcgagaa tctggtcaac ggacgttggg
     3781 aactcgcttg tcgaaatctc ggtactcatg gcttcgaatc ctgtcttcct gttcagatgg
     3841 ggatacgcgt cattctcggg aaaggttgca cggcgatttc tcagcagcga cggaatgcca
     3901 gacctccatc ggacgagaac tcctccgcgg caagcaaggc atcggcgagt tcattcgccc
     3961 accgcgtcgc cggctctgcc ccgtcatacg tcagcacgtc gagttcgccg tccggtggtt
     4021 cgaacatccg gacgagttcg gctaattccg gatcgcccac gtcgggtcgc att
//
LOCUS       DASTMD010000345         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2352444, whole genome shotgun sequence.
ACCESSION   DASTMD010000345 DASTMD010000000
VERSION     DASTMD010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2352444"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..2012
                     /locus_tag="VFQ54_05075"
     CDS             36..2012
                     /locus_tag="VFQ54_05075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0164396.1"
                     /translation="MTTPISPERAIYDIPSLNTPRISPDGSRIAYVRGQVNQETCKSE
                     SQIWISDLDGSNRRRLTWTGTRNGDPVWSPDGSQIAFVTVRDGDRPNSIAILGFDGGE
                     SRVVTSHSVAPGGLAWSPDGTKIAYSAAVDPYNPNETPRDPKAPARVRVVKRIDYKQD
                     GYGFVNDVRSQIFVVDVTSGERTQLTSETIDHGRPAWSPDGSKLAVSLPSMNGMISQI
                     GILDVASAPGPDALRRLGPAEGSTALFAWSPDGTKILFDGSHEVSPHHDYYVADVASG
                     EVTQVTDDIFFLPDVGYQSNGPAGSPVWLDESTALVHGLLNGASGIWKLAIPGGETTE
                     VARWKAMHSGFTVSPDHKTVIQVVSSLDGIVGLNALDVESGASTLLFNEAETVFAETP
                     VAPWELVSIERGGLTIEGWLLAPHNFDPARSYPLILDVHGGPHNAYGYGISAIAEVWA
                     THDFFVLLPNPRGSGTYGRAFAEAVTGDWGNEDWKDLEGILEHVLERPYIDRARTGIY
                     GYSYGGYMTAWVIGNTDRFVTAICGAPVFDSESMFGTSDIGHRFDIEMGGLPWGDGRE
                     QLMKSSPSTYVHNAKTPTLIVQGEADERCPVGQAEQLFISLLKLGVETEFVRYPGGSH
                     LFINSGEPAHRLDYFTRTLAWFKKYLGDPAERAQ"
     gene            2095..>2770
                     /locus_tag="VFQ54_05080"
     CDS             2095..>2770
                     /locus_tag="VFQ54_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164397.1"
                     /translation="MSAAITPTGIIQELVSLSGVGVSPDGAFITYVRTKYSKDSGKKE
                     SQVWIADIDGGNRRQLTYVGGANANPIWAPDSSKIAFTSSREGDKPGSIAILPIAAGG
                     EAKVIQSHLATPVVLKWSPDGTKIAYTVAIDPENPNETPRDPKAPAKVRVVSQIDYKT
                     DGIGYRGETRFQLFILDLETGESRQVTSGLRDILHPTWSPDGKTIAATVLDGAPHLSK
                     LGLIDVA"
BASE COUNT          524 a          955 c          838 g          453 t
ORIGIN      
        1 tcccgtttcg aaaacgcgta gccaaggagg aagagatgac aacaccaatc agtcccgaac
       61 gcgcgatcta cgacatcccg tcgctcaaca caccccgcat ctcgccggat ggctcccgga
      121 tcgcctacgt gcgcgggcag gtcaaccagg agacctgcaa gtccgagagc cagatctgga
      181 tcagcgatct cgacgggtcg aaccgccgcc gtctgacgtg gaccggcacc cgcaacggcg
      241 atcccgtctg gtcacccgat ggctcccaga tcgccttcgt caccgtccgc gacggcgatc
      301 gtccgaactc gatcgcgatc ctcgggttcg acggcggcga atcgcgcgtg gtcacgagcc
      361 acagcgtcgc gccgggcgga ctcgcctggt cgccggacgg cacgaagatc gcctacagcg
      421 cggcggtcga tccgtacaat ccgaacgaga cgccgcgcga tcccaaggcg ccagccaggg
      481 tccgcgtcgt gaagcggatc gactacaaac aggatggcta cggtttcgtc aacgatgtcc
      541 gcagtcagat cttcgtcgtc gatgtgacca gcggcgagcg cacccagctc acgagcgaga
      601 caatcgacca cggtcgaccg gcctggtcgc cggacggatc gaagctcgcg gtctcgctgc
      661 cgagcatgaa cggcatgatc tcccagatcg gcattctcga tgtcgccagt gctccgggtc
      721 cggacgccct gcgccgcctc ggaccagcgg aagggtcgac cgcgctcttc gcatggtctc
      781 ccgacggcac gaagattctc ttcgacggct cccacgaggt cagcccgcac cacgattact
      841 acgtcgccga cgtcgcctcc ggcgaggtaa cccaggtcac ggacgacatc ttcttcctgc
      901 ccgatgtcgg ctaccagtcg aacggccccg ccggttcgcc ggtctggctc gatgaatcga
      961 cggcgctcgt ccacgggctg ctcaacggcg cgtccggcat ctggaagctc gctatcccag
     1021 gcggcgagac cacggaggtc gcgcgctgga aggcgatgca ctccggcttc accgtctccc
     1081 cggaccacaa gacggtcatc caggtggtgt ccagtctcga cggcatcgtc gggctgaacg
     1141 cgctcgacgt ggagtccggc gcatcgacgc tcctcttcaa cgaggcggag acggtcttcg
     1201 cggagacccc ggtcgcgccg tgggaactcg tctcgatcga acgcggcggc ctgacgatcg
     1261 agggatggct gctcgcaccg cacaacttcg accccgcgcg cagctatccc ctgatcctcg
     1321 acgtccacgg cggcccacac aacgcctatg gatacggaat cagcgcgatc gccgaggtct
     1381 gggcaacgca cgacttcttc gtcctgctcc cgaatccgcg cggttccggc acctacggtc
     1441 gcgccttcgc ggaagcggtc accggcgact gggggaacga ggactggaag gatctcgagg
     1501 gcattctcga gcacgtcctc gaacgcccgt acatcgatcg ggcgcggacc ggcatctacg
     1561 gctactccta cggtggctac atgacggcct gggtgatcgg caacaccgat cgcttcgtca
     1621 ccgcgatctg cggcgccccc gtcttcgact cggagtcgat gttcggcacc agcgacatcg
     1681 gtcatcgctt cgatatcgag atgggcggtc tgccctgggg tgacggtcgc gagcagttga
     1741 tgaagtcatc cccatccacc tacgtgcaca atgcgaagac cccgaccttg atcgtgcagg
     1801 gcgaggcaga cgagcgctgc ccggttggtc aagccgagca gttgttcatt tcgctgctca
     1861 agttgggcgt cgagaccgaa ttcgtccgct acccgggtgg atcgcatctc ttcatcaata
     1921 gcggcgagcc ggcgcaccgc ctcgactact tcacgcgcac cctcgcctgg ttcaagaaat
     1981 atcttggcga tccggcagaa cgcgcccaat gaccccatcg acgaggtgaa gatgcgcggt
     2041 gataatccgc gcattcggga ccaatcgttc ggttcgtcta tcaaagagag agtcatgagc
     2101 gcagcgatca cacctaccgg catcattcag gaacttgtca gcctcagcgg cgtcggcgtc
     2161 tcgccggacg gcgcgttcat cacctacgtg cggacgaagt acagcaagga ttccggcaag
     2221 aaggaaagcc aggtctggat cgccgatatc gacggcggca accgtcgcca gctgacctat
     2281 gtcggcggcg cgaacgcgaa tccgatctgg gcgccagaca gcagcaagat cgccttcacc
     2341 agctcccgcg aaggcgacaa gccgggctcg atcgcgatcc ttccgatcgc cgccggtggc
     2401 gaggcgaagg tcattcagtc ccacctcgcg acccccgtcg tgctcaagtg gtcgcctgat
     2461 ggcacgaaga tcgcctacac agtcgcgatc gatccggaga atccgaacga aacgccgcgc
     2521 gacccgaagg cgccggccaa ggtccgcgtc gtcagccaga tcgactacaa gacggacggc
     2581 atcggctatc gcggcgagac ccgcttccag ctcttcatcc tcgatctcga gacgggcgag
     2641 agtcgccagg tgacgtccgg cctccgcgac atcctgcatc cgacctggtc acccgacggc
     2701 aagacgatcg ccgcgaccgt cctcgacggc gcgccgcatc tctccaagct cggcctgatc
     2761 gatgtcgcct
//
LOCUS       DASTMD010000346         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_924322, whole genome shotgun sequence.
ACCESSION   DASTMD010000346 DASTMD010000000
VERSION     DASTMD010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924322"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VFQ54_05085"
     CDS             complement(<1..1086)
                     /locus_tag="VFQ54_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164398.1"
                     /translation="MRRMTWMALGAVFAMLTATFATSSIAWPGTSAGVAIVAAQEAPD
                     CSGIVANGDFESPEISGGQNEDQQSDADFWDGDYWLTGYDPAYSGNQSVYIREGGDMS
                     QTLDTSGLVAGDTITISFQHDLDITASLNDSSDSWTGGTFEEYQPASVSYTLTSDAET
                     VTLTFAGTVGDVGSSVDYVTAACSYTAPTPTTTPTLEPTETATTAPTVEPSATTTATI
                     ESTATSIATTTPTVGATETPGTVSPTTSASVTPTMEMTAEPTTEPTGTGSAAVTLSSS
                     DGGAIPDDAIVCVGDACQSVGSGASAAAVSGTTLSFDALAPGTYPVTVTGAAPYADAT
                     ASVTIVAGSTSEVAITLQLTVAPTQPGA"
     gene            complement(1161..1331)
                     /locus_tag="VFQ54_05090"
     CDS             complement(1161..1331)
                     /locus_tag="VFQ54_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164399.1"
                     /translation="MNRRGIRPTAVQFAGRDELARRLPLVLANPRPSRREIDLYAVQM
                     PKITIAFRGIFA"
     gene            complement(1435..>3457)
                     /locus_tag="VFQ54_05095"
     CDS             complement(1435..>3457)
                     /locus_tag="VFQ54_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164400.1"
                     /translation="GKRGITGPRAFASSRKVSLGLVTSLLLAMLSVIAVPTGAGAQTA
                     PPFGAAAAPDLSFDRDAYGVFAGSPTVTTLTVSIDTAIDAGLSISTSPTGHFTFTGVT
                     VVGFGGTTCTLFGGTSPSIGINVVSPGTGGCTVTIPVDRVLGTADGTMAVMTASLGDG
                     SANSTLVAATTPTETPPATATITPTPTQTLTPIPTDTATTIPPTATPAGSQAFDQSTY
                     ALPVDGSVTAILTITLTGPTDLSTISIIGPAPLLTAAITSVATTGGVIYTVQSNGGNV
                     SGTVSSPNAGTCTITLTLTTNPAFPDLEGSIIPLTSIVQGLLSAISTIHVGPASPTAT
                     LTPLPTDTAVPTDTATIAPTETATATGTAIPTDTVTIAPTETATATETAIPTDTVTIA
                     PTETPVVTPSPIATDSPTVVPTATATIVSTGTVTTVPTAVPTSTATTGPTTTVVPPTE
                     IATLLPTGTATTVSTETVPTATEILATSTTAPPTGTVAITGSITFTLSTADGGNVPDS
                     TQVCVGGGTDVCQTIGASLSAAAVSPTTLTFADLAPGTYDVTVTNAAPYQDTAGSVVV
                     TAGEATTSDIVLQLAEVTTTVEPTQPGAATPIATGTITPTQAPANGGGGGAAPAKPNV
                     PSGGAPAVKALPNTGAGGGSSGASLTMLVLAIIAVIGAGVLNWRRRRTS"
BASE COUNT          520 a         1049 c         1216 g          672 t
ORIGIN      
        1 ggcgcctggc tgcgtcggtg ccacggtcag ctgcagggtg atcgcgacct cgcttgtcga
       61 gccggcgacg atcgtcacgg aagcggtggc gtcggcatac ggagcggcgc ccgtgacggt
      121 gaccggatag gttccgggcg ccagcgcgtc gaagctcagc gtggtgccgg acactgccgc
      181 ggcactcgca ccgctgccca cgctctggca ggcgtcgccg acgcagacga tggcgtcgtc
      241 cggaatcgct cccccatcgc ttgatgagag cgtcacggcc gcggatcccg tcccggtcgg
      301 ttccgtggtg ggttcggcgg tcatctccat cgtcggtgtg accgatgcgg aggtggtggg
      361 cgaaactgtt cccggcgtct cggtggcgcc gaccgttggt gtcgtcgttg cgatgctcgt
      421 cgccgttgac tcgatcgttg ctgtcgttgt ggccgacggc tcaacggtcg gtgccgttgt
      481 cgcggtttcg gtcggctcga gagtgggtgt cgtcgttgga gtgggcgcgg tatacgagca
      541 tgctgccgtc acgtaatcga ccgacgaccc cacgtctcca actgttcccg cgaatgtcag
      601 cgtcacggtc tcggcatccg aggtcagggt gtagctcacg ctggcgggct gatattcctc
      661 gaatgtgccg cccgtccaac tatcggagct gtcgttgaga ctggcggtga tatccagatc
      721 gtgctgaaag gaaatcgtga tggtatcccc ggcaaccaga ccgctcgtat cgagtgtctg
      781 cgacatgtcc ccgccctcgc ggatatagac gctttgattg ccgctgtagg ctgggtcata
      841 tccagtgagc cagtaatcgc catcccagaa atcggcatcg ctctgctgat cttcgttctg
      901 gccgccgctg atctccgggc tctcgaagtc gccgttggcg acgataccgg agcaatccgg
      961 ggcttcctgt gcggctacga tcgcgactcc ggctgacgtg ccgggccatg caatggacga
     1021 tgtggcgaac gttgccgtca acatggcgaa cacggcgccg agcgccatcc aggtcatgcg
     1081 tctcatgatt cccctcccac gacgatgcgt tcgtggacgc aatgcgtctg cgtgtaccgc
     1141 gtttgatccc cacggacact ttaggcaaag atgccccgga acgcaatggt gatcttcggc
     1201 atctggaccg catataagtc gatctctctc cgcgatggcc ggggattcgc cagaacgagg
     1261 ggcaagcggc gggcaagctc gtcgcgaccg gcaaactgaa ccgccgtggg acgaatccca
     1321 cggcggttca tgcgttcgct catggcgagc tatgtcaggg cggattcccg tgtccgcaac
     1381 gacgcggaca gccgcctccc tcattcatcg tgcgctcgat ctcgtcgtga ggcgttacga
     1441 cgtccggcgg cggcgccagt tcaggacacc tgccccgatc acggcgatga tcgcaaggac
     1501 gagcatcgtc aggctggcgc cactcgaccc gccaccggca cccgtgttcg gcagtgcctt
     1561 cacggccgga gccccgcccg acggtacgtt cggcttcgcc ggggcagcgc caccaccacc
     1621 accgtttgcc ggcgcctggg tcggagtgat cgttccagtc gcgatcggcg ttgcggcccc
     1681 gggctgcgtc ggctccaccg tcgtcgtgac ttcggccagc tggagcacga tatcactcgt
     1741 cgtggcctca cccgcggtga cgaccacgct gcctgcggtg tcctggtacg gtgccgcgtt
     1801 cgtgacggtc acgtcatacg ttccgggcgc gaggtcagca aacgtcaggg tcgtcggtga
     1861 gaccgccgcc gcgctcaggc tggcgccgat ggtctgacag acatcagtgc caccaccaac
     1921 acagacctgg gtcgagtccg ggacgttgcc gccgtcagcc gtcgagagtg tgaacgtgat
     1981 cgatccggtg atggcgacgg tccctgttgg tggagccgtc gtgctggtgg cgagaatctc
     2041 ggtggcagtc gggaccgtct ccgtcgaaac ggtcgtcgcg gtgccggttg gaagcagggt
     2101 tgcgatctcc gtcggcggca cgaccgtcgt cgtgggacct gtcgtggccg tggaggtcgg
     2161 cactgcggtc ggcaccgtgg tcacggtccc tgtcgagaca atcgtcgcgg tggcggtggg
     2221 cacgactgtc gggctgtcgg tggctatcgg tgacggcgta accaccgggg tctcggtcgg
     2281 ggcgatggtg acagtatccg tgggtatcgc ggtctcggtc gccgtcgcgg tctcggtcgg
     2341 ggcgatggtg acagtatccg tgggtatcgc ggtcccggtc gccgtcgcgg tctcggtcgg
     2401 ggcgatggtg gcagtatccg tgggtaccgc ggtgtcggtt ggcagcggtg tcagggtcgc
     2461 ggtgggactg gccggtccaa catggatcgt cgagattgcg gagagcaatc cctgaacgat
     2521 tgaggtcaga ggaatgatgg acccctcaag atcggggaac gccgggttcg tggtcagcgt
     2581 cagcgtgatc gtgcaggttc ccgcgttcgg tgacgagaca gtgccgctga cgttgccgcc
     2641 attggactga accgtgtata tgacgccccc ggtcgtcgcc acggaggtaa tggccgccgt
     2701 caggagtggc gcggggccga ttatcgagat cgtcgacaaa tcggttgggc cggtcagcgt
     2761 gatcgtgagg atcgctgtga cgctgccatc taccggaagt gcgtaggtgg attggtcgaa
     2821 cgcctggcta cctgccggcg tcgctgttgg cggaatcgtc gtcgccgtgt ccgtcgggat
     2881 cggggtcagc gtctgggtcg gcgtgggggt gatcgtcgcg gtggcgggcg gcgtctctgt
     2941 cggagtcgtc gcggcgacga gcgtggagtt ggcagacccg tcgccaagcg aggcggtcat
     3001 cacggccatc gtgccgtcgg ccgttccgag cacgcggtcg actggaatcg tgacggtgca
     3061 tcccccggtt cctggcgata cgacgttgat gccgatcgat ggggaggttc ctccaaagag
     3121 tgtgcacgtc gtcccgccga agccgaccac agtgaccccg gtgaacgtga agtggccggt
     3181 ggggctggtg ctgatcgaca agccggcgtc gattgcggta tcgatcgaca cggtcaacgt
     3241 ggtgacggtt ggtgatccgg cgaagactcc gtatgcgtca cggtcgaagc tcagatccgg
     3301 tgcggcggct gccccgaatg gtggtgctgt ctgcgccccc gcgcccgtcg gcacggcgat
     3361 gacgctcagc attgcgagca gcagtgatgt gacaagaccg agagagacct tccttgacga
     3421 agcgaacgct cgcggacccg tgattccccg tttcccc
//
LOCUS       DASTMD010000347         1698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1014383, whole genome shotgun sequence.
ACCESSION   DASTMD010000347 DASTMD010000000
VERSION     DASTMD010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014383"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..1427
                     /gene="ispG"
                     /locus_tag="VFQ54_05100"
                     /gene_synonym="gcpE"
     CDS             249..1427
                     /gene="ispG"
                     /locus_tag="VFQ54_05100"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU0164401.1"
                     /translation="MSLVAPRRKTRVLHVGDVALGGEYPIRVQSMATADPRDPKAVLA
                     QIKDLADVGCEIVRIAVPNREAAAALPEIVPYAAVPLIADIHFEHTLALKAIEAGIHG
                     LRLNPGNIRKPEDVREVVAKAKERQIPIRIGVNFGSVAPMTEEFVDEMAQQNATQVEL
                     RAEWLVRSALGHIRILEDLDFYDTKVSLKAFEVPVLLEAYQRFARLPNDYPLHLGVTE
                     AGTPRAGSVRSAVGIGTLLALGIGDTIRVSLTTDPKEEVFVGYEILKSLELRQKGATM
                     IACPTCGRVEVDLFALANKVDLVLEKIDEPIRVAVMGCVVNGPGEARDADVGIASGNG
                     KGVIFRKGKIVKTVLEVDMLDELTKEIHAVVEDRKNGIFDEADSHANAYKPMISLTAV
                     "
BASE COUNT          313 a          557 c          513 g          315 t
ORIGIN      
        1 gcgtgatgtt cttcgtcgcc ttcggcgatg tctcccgcct cttcgacggt acggcgctcc
       61 ttccgtaaac aggatccggg gcatcgcgat cgtcggagtt gcaggccaaa gatcaacgga
      121 tcgtgaacgc aattcccgcc cgctgggcgt atcattggta aggagatgtg tgcgccgccc
      181 tgccagagtg gcgatatgac ctgcgacgca caaacacagc gcctatccat ctcgtggaga
      241 taccaacgat gtcactcgta gcacctcgac gcaagactcg tgtccttcac gtcggcgacg
      301 tcgcccttgg tggcgagtat ccgattcgcg tgcagtcgat ggcgaccgcc gatccccgtg
      361 atccgaaggc cgtcctcgcc cagatcaagg atctcgccga tgtcggttgc gagatcgtgc
      421 gcattgccgt tccgaaccgg gaggctgccg cggcactccc cgagatcgtt ccctatgccg
      481 ccgtcccgct gatcgcggac atccacttcg agcacaccct cgccctgaag gcgatcgaag
      541 ccggcatcca cggcctccgc ctcaatcccg gcaacatccg gaagccggaa gatgttcgcg
      601 aggtcgtcgc gaaggcgaag gaacgccaga ttccgatccg gatcggcgtc aacttcggtt
      661 cggtcgcgcc gatgaccgaa gagttcgtcg acgagatggc ccagcagaac gcgacgcagg
      721 tcgagttgcg cgcggagtgg ctcgtccgct cggcgctcgg tcacatcagg atcctcgagg
      781 atctcgattt ctacgacacc aaggtcagcc tcaaggcctt cgaggttccg gttctcctcg
      841 aggcgtacca gcgcttcgcc cgccttccga acgactaccc gctccacctc ggcgtgaccg
      901 aagccggtac gccgcgcgcg ggcagcgtgc gttcggccgt cggcatcggc accctcctcg
      961 cgctcggcat cggcgacacg attcgtgtct cgctgacgac cgatccgaag gaagaggtct
     1021 tcgtcggcta cgagatcctg aagagcctcg agctgcgaca gaagggcgcg acgatgatcg
     1081 cctgcccgac ctgcggtcgt gtcgaggtcg atctctttgc gctggcgaac aaggtcgatc
     1141 tcgtcctcga gaagatcgac gagccgattc gcgtcgccgt catgggctgc gtcgtgaacg
     1201 gtcccggcga ggcccgggac gccgatgtcg ggatcgcgtc gggcaatggc aagggcgtga
     1261 tcttccgcaa gggcaagatc gtgaagaccg tcctcgaggt cgatatgctc gatgaactca
     1321 ccaaggaaat ccacgccgtc gtcgaggacc gcaagaacgg catcttcgac gaggcggatt
     1381 cgcatgcaaa cgcatacaag ccgatgatct ccctcaccgc cgtctaggga tcgatcgaca
     1441 cagcagaaga atgcccgcga tcggtccgat cgcgggcatt cttcgtctgc cggcatgctg
     1501 ccatgtggtc cgggtcgcga ccggcacgac gacgccgatt catgcgacca tagcgcgcgg
     1561 cgcgcacgca ccacgatcgg gcgctcgtgc ccaaactctt gcacacttcg tgatctttgt
     1621 cacaatcttt cgcgaagcat cctatactac ggctgcctga agttcggtct ggctgtgaga
     1681 ccgctggcgc ctgtatcc
//
LOCUS       DASTMD010000348         1840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1819346, whole genome shotgun sequence.
ACCESSION   DASTMD010000348 DASTMD010000000
VERSION     DASTMD010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819346"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            539..1750
                     /locus_tag="VFQ54_05105"
     CDS             539..1750
                     /locus_tag="VFQ54_05105"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU0164402.1"
                     /translation="MTSSPSTIEPTKLFDYSVQTFLVGDEIALRPVVKEDAPFTASWR
                     GSLYPRSPSNTETWIEKKIGGDAGNPYVIVRKSDGRPVGLYGTEAAFPAVWNHVFIDP
                     LFATQELAWTIEAWRLIVPWQIGEKSTIILRQVVPGDETDLLAALVADGWRESARYRD
                     FLWSRQAATWVDRVVLEYLSPQWVALLGDPAEVPLERTGLGTPRPVPALVATPEKVPP
                     QAIMVGERVYLRPLNKADGVTIAEMTRKEPDPIWAIGRRLAYPVSYAGWTDKLQEKEP
                     QEWVRFAVCLRENDRLIGSMGLYGIDYLHGFAESESEIYDPEYRGGGYGSEAKHLLFA
                     YAFDRLGLHALKSWVIFQNTRSAAALRKQGYVEAGRHHWAYPIDGGFGNAVVFTLHAD
                     TWRALPRTSAE"
BASE COUNT          329 a          592 c          570 g          349 t
ORIGIN      
        1 gggcgagcac caggctcgcc cctacggccg cggggattgc gcgacaggca cgtggtgtcg
       61 acgacgtgcc tggggaggtg ttggagaggc cgcgcggtga ccgggtgaca acgcggttgg
      121 gatcaggtat catgccagac agtgaaggtg tattcatgcg ccttttacct ctcatggatc
      181 catgccagtc gccactggcc ggcagtcgat ccggtgttcc ctgtcctcac aggcgatgaa
      241 cccgggttcg ttatgaaagg gggtgggttt gatgacaaca tccagcgtcc accacatcac
      301 aacgcccgca acatcgggaa tccaccgtta actcctcaac ggtgcttccg gatcacctct
      361 tcacgcgtcg ctcgttccat ctgaactgac ccgctgccca tcatggcgaa gctgtgctcg
      421 cttgcgagcg ccgccccgct gttgtcaggg ctccgatcgc gacgccccgt tcccatcccg
      481 attacccggc gcggcctcac tgtgtgggtg ccctgcgcga tgaatatccg aggcgaacat
      541 gacgtcttct ccatcaacca tcgagccgac gaagctcttc gactactcgg tccagacctt
      601 cctcgtcggc gacgagattg ccctgcgacc agtcgtgaag gaagatgctc ccttcaccgc
      661 gtcctggcgt ggcagcctct acccccgctc gccatcgaac accgaaacct ggatcgagaa
      721 gaagatcggc ggcgacgccg gcaatcccta tgtcattgtc cggaagagcg atggtcgtcc
      781 cgtcggtctc tatggaaccg aggcggcgtt tcccgcggtc tggaatcacg tcttcatcga
      841 tccactcttc gcgacgcagg aactcgcctg gacgatcgag gcatggcggc tgatcgtgcc
      901 gtggcagatc ggggagaaga gcacgatcat cctccgccag gttgtcccgg gtgacgagac
      961 tgacctgctc gcggcgttgg tcgccgacgg ctggcgcgaa tctgctcgct accgcgactt
     1021 cttgtggtcg cggcaggcgg caacctgggt cgatcgcgtc gtgctcgagt acctgagccc
     1081 gcaatgggtc gcgctgctcg gtgatcccgc tgaggttccg ctcgagcgaa ccggtctcgg
     1141 cacgccacgg ccggttcccg cgctcgtcgc gacaccagag aaggtcccgc cccaggcgat
     1201 catggtcggg gagcgcgtct acctgcgacc gctgaacaag gccgacggtg tgacgatcgc
     1261 cgagatgacc cgcaaggagc ctgatccgat ctgggcgatc ggtcgacgcc tcgcctatcc
     1321 cgtcagctac gccgggtgga cagacaagct gcaggaaaag gagccacagg agtgggtgcg
     1381 cttcgcggtc tgccttcgtg agaacgatcg gctgatcggc tcgatgggac tctatggaat
     1441 cgactatctc catggcttcg ccgagtcgga gagcgaaatc tacgaccccg agtatcgtgg
     1501 cggcggctac ggctccgagg cgaagcatct gctcttcgcc tatgcattcg atcgtctcgg
     1561 gctgcacgcg ttgaagagct gggtgatctt ccagaatacg cgctcggcgg ccgcgctacg
     1621 gaagcaggga tacgtcgaag cgggccgcca tcactgggcc tatcccattg atggcggatt
     1681 cggcaatgcc gtcgtcttca ctctccatgc cgatacctgg cgcgcactgc cgcgcacaag
     1741 cgccgagtaa ttcgccgcct gtccatccac gaagaggtga ttcgtgcctg acgttgcaat
     1801 tgaaccaaag aagatcgccg gtccggcgct ccagaccgcg
//
LOCUS       DASTMD010000349         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2622056, whole genome shotgun sequence.
ACCESSION   DASTMD010000349 DASTMD010000000
VERSION     DASTMD010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2622056"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VFQ54_05110"
     CDS             <1..847
                     /locus_tag="VFQ54_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0164403.1"
                     /translation="MLGWFVAGRVLRPIRRITAHARRASSANLDERIDSRGPPDELHE
                     LSETFDAMLDRLQTAFQAQRQFAGQVSHELRTPLAIMQAAADVTLADPNATAHERELG
                     LTIRNSVSRSERLIDGLLILSRSESTLLDTTTLDLAEIAGNCLANAARAAGAAGITID
                     ESELAPAPVRGDRLLLERLCDNLIDNAIRYNRTDGSGWLRVSTSVDEDFSELKVANSG
                     SWIMETTIDRLFAPFQRGGRPDIPGYGLGLAIVRSVTAAHGGTIHARPLPGGGLEISV
                     RLPRV"
     gene            complement(945..1940)
                     /locus_tag="VFQ54_05115"
     CDS             complement(945..1940)
                     /locus_tag="VFQ54_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164404.1"
                     /translation="MSIIEDPTPLARTSILNNVTRRRLIGSAAGLAAATLATTGLVSA
                     QTATATPDASSSSSTDSTTSTSDLAASKDDQLTTFLASVKTDRDAVASSIDTTMIDQI
                     LMVANDLQTQSTTASDTDSPPVPSDSSDSTSSSATPAASSSSSSSTASASTTVAPGKQ
                     QLIAAARSSGSAARQLILAGLSAYGLPSQQNAASRSLAAVYDRIKTAGTDVSGSSIAD
                     ATTLVTYAQQLYSDAYDTYGAGTYAQAQGTGSAAGGLAGAAETLLGKGGGFGGAGGRD
                     LQGGDGPGGGMPASDGQRGGMNQKDSTGGSSSGGDSSAPGDTDSSTPTTVPTPSF"
BASE COUNT          463 a          775 c          801 g          475 t
ORIGIN      
        1 gatgctcggc tggttcgtcg ccggtcgcgt cctccgcccg attcgccgga tcaccgccca
       61 cgcccgccgg gcgtcgtccg cgaatctcga tgaacgcatc gattcacgcg gaccgccgga
      121 tgaactgcac gagctttccg agaccttcga cgcgatgctc gaccgtcttc agaccgcgtt
      181 ccaggcacag cggcaattcg cggggcaggt ttcccacgag ctgcggacgc cgctcgcgat
      241 catgcaggcg gcggcggatg tgacgcttgc cgatccgaat gcgacggcgc acgagcgtga
      301 gttgggattg acgatccgca attccgtcag ccggagcgag cgcctgatcg acggcctgct
      361 gatcctctcg cgcagtgaga gcacgctgct cgacacgacc acgctcgacc tggccgagat
      421 cgccgggaac tgtctcgcga acgccgcccg tgccgccggt gcggccggga tcacgatcga
      481 tgagagcgaa ctcgctcccg cgccggtgcg tggcgatcgg cttctgctcg aacgtctctg
      541 cgacaacctg atcgacaacg cgatccgcta caaccggact gatggctcgg gttggctccg
      601 cgtctcgaca tcggtcgacg aggacttctc ggaactcaag gttgcgaaca gcggctcctg
      661 gatcatggag acgacgatcg accgcctctt cgctccattc cagcggggag ggcgcccgga
      721 tatccccggc tacgggcttg gcctggcgat cgtccgctcc gtcaccgccg cccacggcgg
      781 gacgattcac gcacgacctt tgccgggcgg cggtctggaa atctcggttc gcctgccgcg
      841 ggtctgaaga cgacgcttca gacgacgcgc gcgaatcgga gataccggac gcggaaaggc
      901 ggcgcccgaa ggcaccgcct gtgtgaaatt ctcgaggggt tcgatcagaa cgagggcgtc
      961 gggacggtcg ttggtgtcga gctgtcggta tcgcccggtg ctgacgaatc gccgccggag
     1021 gatgacccgc ccgtcgaatc cttctggttc ataccgccac gctggccatc agaagccggc
     1081 attccgccgc caggaccatc gccaccctgg agatcgcggc caccagcacc accaaagccg
     1141 ccgcccttgc cgagcaacgt ctcggccgca ccagcgagac cacccgccgc gctgcccgtg
     1201 ccctgggcct gcgcgtaggt gccggcgcca tacgtgtcat acgcatccga gtagagctgc
     1261 tgggcatagg taacgagcgt cgtcgcatcg gcgatcgagc taccggagac gtcggtaccg
     1321 gccgtcttga tccgatcgta gacggcggca agcgaacgac tcgccgcgtt ctgttgcgag
     1381 ggaaggccgt atgccgagag cccggcgagg atcagttgcc gcgccgcgga gccagaggac
     1441 cgcgcggccg cgatcagttg ctgcttcccg ggcgcgaccg tggtcgacgc agacgcggtc
     1501 gaggaggaac tcgaagacga agcggccggc gtcgccgagg aggacgtgga atcgctggaa
     1561 tcgctcggca ccggcggcga atcggtatcg gacgcggtcg tcgattgggt ctggaggtcg
     1621 ttcgcgacca tgaggatctg atcgatcatc gtggtgtcga tcgaagaggc aacggcatca
     1681 cgatccgtct tgacggaggc gaggaaggtc gtgagctgat cgtccttcga cgcggccagg
     1741 tcggacgtgc tcgtggtgga atcggtcgac gaggaactcg acgcgtccgg tgttgcggtc
     1801 gccgtctggg cggaaacgag cccggtggtg gcaagcgtcg ccgcggcgag accggcggcg
     1861 ctgccgatca accgcctgcg ggtcacattg ttgaggatgc ttgtcctcgc gaggggagta
     1921 gggtcttcga tgatgctcat gccatgactc ctggcgtgat gaatggaagg acggtgtcga
     1981 gatccgttcg tgctccatcg acacgttcgt ctctcgacat ccccaaccta gtccccaccg
     2041 gatatggccg agatatggtc agcgccgtga ttttccgcag aacggcgcgg attcccgatt
     2101 cgtagcgtaa agtccactgc aagatcgacg tttgggactc atcctccgtt ttccatgttg
     2161 ttgcatatca tgagtcgatc tgtatacatg tcatgatatc attttggacg attcatatat
     2221 gtggagtcat ttgtaacgaa ccgaatttcc gcgtcgttcc gggccgttgg gaatctcgtg
     2281 aatcttgatc tcgatgcagc ggcaacattc acgaagaaaa aagcccgcca aatcaaccat
     2341 tttcagctgt tccgtgaatc tcgtgaatgt tgtgaatgtt ctctcttagg actcagacga
     2401 agtgtttaca catcatccta gaaacgtata aggaaaacat tcacgagatt cacgaagatt
     2461 caccgcctga cccgattcgg cccggaacgg cgggcttttt cgcctcgtga atct
//
LOCUS       DASTMD010000350         1584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2264601, whole genome shotgun sequence.
ACCESSION   DASTMD010000350 DASTMD010000000
VERSION     DASTMD010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2264601"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFQ54_05120"
     CDS             complement(<1..207)
                     /locus_tag="VFQ54_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164405.1"
                     /translation="MTDLRQIEIDYRRIGPTTLAVWRSELVRVGSPLLPNAEAMWRVA
                     GDHSALALAQMWIEDQYETDRKLLA"
     gene            708..1073
                     /locus_tag="VFQ54_05125"
     CDS             708..1073
                     /locus_tag="VFQ54_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164406.1"
                     /translation="MLKQTGYELDFPFWDDDETQPIIGFTTFARDLMSPDDIQTATIQ
                     YTPEDSKEIETYPHPNGLDTVARYNSASLVKMMATFDSAGLNWFPNGDPGDFVLILHP
                     ADADPSRIDTVDVGLGNNP"
BASE COUNT          337 a          501 c          441 g          305 t
ORIGIN      
        1 ggcgaggagc ttgcgatcgg tctcgtactg gtcctcgatc cacatctggg ccagggccag
       61 cgcggagtga tcgcccgcca cccgccacat cgcctcggca ttcggcagca acggactccc
      121 gacgcggacc aactccgacc gccagacggc gagtgtggtc ggcccgatcc ggcggtaatc
      181 gatctcgatc tgacgcaggt ctgtcatggc ggttccctct cggatgcgtt cgcgcgacga
      241 tctctctatc cggcgaacgc agcgaaaacg acaaaacgag ccacgttttg acggaaccgc
      301 gtcatcgatc gccacggttc agcgatgcgt tgatctcctc acggcactcc tctcctataa
      361 tgagcccgcc ttcacccccg atcgacgtgt gccagacgca cagggaatca aggatccgaa
      421 tcagtgatga caacactcga ttccagtttc gacctgatcg acgtcatagg ccgatgaatg
      481 gtgtcgtcgg cacggatagg agaatttagg tgcagatttc tcgaagatca tttgttgcac
      541 agtcgatatt cgtgagcgct gcgattgcaa cgtttgccgg gtcagccagc gtccttgcga
      601 agacgccaaa gccgaaacgt ggcggacttg ggcattcaga gaaggtttgg atcaagcttt
      661 gggggaagcc ggatgaaacg cccattgcga gcggccaggc tccctatgtg ctcaagcaga
      721 caggatacga gctcgacttc ccgttctggg atgatgacga gacgcagccg atcatcggat
      781 tcacgacctt cgcgagggac ctgatgtcgc cagacgacat ccagacggcg acgattcaat
      841 acacgcccga ggattcgaag gagatcgaga cctatcctca tcctaacggc ctcgataccg
      901 tcgcacgata caacagtgcg tcgcttgtca agatgatggc aacgtttgat tcagccggac
      961 tcaattggtt cccgaacggt gatcccggag attttgtcct gatcctccat cctgccgacg
     1021 ctgacccgag caggatcgac acggtcgatg tcgggctcgg aaacaatccg taggtcaaga
     1081 cggcggtgat ggccatagcc cacgactgtt accacaaaga gggacaccgt tgacattatg
     1141 acgagaagcg cgaccggtag ctcagcaaca tgcatagccg cggtgttcga gccggtatcg
     1201 tgctaaagcc cgtctacaac tcgacacgta tggtcatcca ggaacgtgcg cacctggaca
     1261 tcctctcagc agcgccggcg gggctcatgc ccgccggctt ctccatgtcc gggccgcatc
     1321 gacccgatct cgatgcggct tgacgattcc tcctccgtcg gcgtgaatac aacgtcaacc
     1381 atcgtcaagc acatgacgcc tcgcgtcatc ccgacagccg cgcaccagct gcagaggaga
     1441 ctccatggcc agctccgatt tcgagttcat cgatccccgc ttcggcaacc tcgtcaaggg
     1501 gtccgccaag gtcgaacacc tcttcaccgg ctcccgctgg gcggaaggtc ccgcctggtt
     1561 ccccgcccac cgctccctca tctg
//
LOCUS       DASTMD010000351         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1462284, whole genome shotgun sequence.
ACCESSION   DASTMD010000351 DASTMD010000000
VERSION     DASTMD010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462284"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFQ54_05130"
     CDS             <1..204
                     /locus_tag="VFQ54_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164407.1"
                     /translation="VTTVVATAGDQISIASRFNDVDTPMASLAVPRGETTQMIAFATD
                     GGELWIHVIAAPSTQWKVLIYVR"
     gene            complement(208..903)
                     /locus_tag="VFQ54_05135"
     CDS             complement(208..903)
                     /locus_tag="VFQ54_05135"
                     /inference="COORDINATES: protein motif:HMM:NF039820.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HEU0164408.1"
                     /translation="MQGNDGFIASTTSHIRRVHDLLREIQRELEDRGEAHDASKLREP
                     EVSLFDQAYADLGAVEYGSEGYQNALVQARQALDHHYAHNRHHPEHFPGGVNDMTLVD
                     LLEMLADWKAASERRPDAEIHEGIRINAERFNLSPQLANIFLNTAVAWWGGPEHAADH
                     IADPGSVPVEPSAPTERDIFAAACERIWSAYQGSLEGRTIEEIAIEIGLEPVNVEHVV
                     LAVSRLLERERHA"
     gene            1207..1998
                     /locus_tag="VFQ54_05140"
     CDS             1207..1998
                     /locus_tag="VFQ54_05140"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEU0164409.1"
                     /translation="MRPISRPRPLLPAQPALNDVSRPDWLRASLIAGFIATFSMTCSI
                     AIAYGIANYYGDAGGNHVERWLQALSHNELTRHIGDRFFVGMILNLLMGIVWAVVYAR
                     VAEPHLSGPGWSRGVVFSLLPFILSIALFFPLAGIGLFGSGVDAGILPVAGNLILHLA
                     YGAVLGSLYAIDPYAGLTDAHGESEAAESAERGAVIGILAGVILGFAGGWIVGPSMDG
                     LASRAIIGVAGSLSGAAIGILIGSLLGMKVLPLDTDNDDRSSKTT"
     gene            2324..>2911
                     /locus_tag="VFQ54_05145"
     CDS             2324..>2911
                     /locus_tag="VFQ54_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164410.1"
                     /translation="MARYLIGRVAGLVFVLFAVSIIAFALMQAVPGGPFGANEKGGSM
                     AAETLKVQKHKYGLDQPVYVQYVKYMYHAVQGDFGVPFQSPTETVTGLIARAWPATLK
                     IGICTILLAYSVGTLFGFLAARHQNGIVDYVVSFTATLGIVLPSFVVAMWMVLIFSIY
                     LNWLPSGGWGSWKEYIMPVIAYSLAPMALVARYTRG"
BASE COUNT          507 a          955 c          867 g          582 t
ORIGIN      
        1 gtgacgacgg tcgtcgccac tgctggcgac cagatttcaa tcgccagccg cttcaacgac
       61 gtcgacacgc cgatggcgtc ccttgccgtt ccccgtggcg agacgacgca gatgatcgcg
      121 ttcgccaccg acggcggcga actctggatc cacgtgatcg ccgctccctc cacccaatgg
      181 aaggtactga tctacgtgag gtaacgctca ggcgtggcgt tcgcgctcaa gcaatcgtga
      241 gaccgcgagc acgacatgct cgacgttgac cggctccagc ccgatctcga tcgcgatctc
      301 ctcgatcgtg cgtccctcga gactgccctg gtaggcgctc cagatgcgct cgcacgcggc
      361 ggcgaagatg tcgcgctcgg tcggcgcgct cggttcaacg ggaactgacc cgggatcggc
      421 gatatgatcc gccgcgtgtt ccggtcctcc ccaccacgca accgcggtat tgaggaagat
      481 gttcgcgagc tgcggcgaga ggttgaaacg ctcggcgttg atgcggattc cctcatggat
      541 ctcggcatcc ggacgccgct cgcttgccgc tttccagtcg gcgagcatct cgaggagatc
      601 cacgagggtc atgtcattca cgccgcccgg gaaatgctcc gggtggtggc gattgtgggc
      661 gtaatgatgg tcgagtgcct ggcgcgcctg gacgagtgca ttctgatacc cctcgcttcc
      721 atattcgaca gcgccaagat cggcatacgc ctgatcgaag aggctgactt cgggctcgcg
      781 gagtttgctc gcgtcgtggg cttccccccg atcctcaagc tcacgctgga tttctcgcag
      841 aagatcgtgg acgcgtcgaa tgtgcgaggt tgtactggcg atgaaaccgt cgttgccttg
      901 caaaaacgct ctccagaatc gcggacgcct cgcacgcggg accccccacc gcgagaaagt
      961 ggattggcga gactataccg cagtcagacc tgcaaatcga ttccgatcat tcctggagcg
     1021 caggaccggg tatatcagac ccgatcgtgg tggcggcgcg acgaccgcgg aggtcgccac
     1081 ggcgcgatgt ccggcgtgac gctgacccgg aagagtcagt tccatcaccc gggtggcgtt
     1141 cttcttgcag cggttatgta cacatggagg taggggagtc tcgataccac cacgagggga
     1201 tgcaccatgc gaccgatctc tcgaccacgc ccactccttc ccgcgcaacc cgccctgaac
     1261 gacgtctcgc ggccagactg gctgcgcgcg agcctcatcg cgggtttcat cgcgaccttc
     1321 tcgatgacgt gctcgatcgc catcgcctat ggcatcgcga actactatgg cgacgccggt
     1381 ggcaaccacg tcgagcgctg gctacaggcg ctgagccaca acgaactgac gcgccacatc
     1441 ggcgatcgct tcttcgtcgg gatgatcctc aatctcctga tgggcatcgt ctgggcagtc
     1501 gtctacgcgc gcgtcgccga gcctcacctc agcggtccgg gctggagtcg cggcgtggtc
     1561 ttctccctgt tgccattcat tctctcgatc gccctcttct tcccgctcgc gggaatcggc
     1621 ctgttcggct ccggcgtgga cgccggaatc ctccccgtcg ccgggaacct gatcctccac
     1681 ctcgcctacg gcgcggtgct cggctcgctc tacgcaatcg acccgtacgc cggtctcacc
     1741 gacgctcatg gtgaatccga ggctgccgaa tcagcggaac gtggcgccgt gatcgggatc
     1801 ctggccggcg tcatcctcgg ctttgccggt ggctggatcg tcggcccgag catggacggt
     1861 ctcgcgagcc gcgcgatcat cggggtcgcc ggctcgctct ccggcgccgc gatcgggatt
     1921 ctgatcggct cgctgctcgg catgaaagtc ctcccgctgg acacggacaa cgacgacaga
     1981 tcgtcgaaaa caacctaggc aatacgtcca aatcaaatcc gacttcaaaa cagaacgaac
     2041 gtgatcgtgg gaccggaatg gcgcttttgc atcatcatcc ggtcccgcgt ttgttcataa
     2101 ccaccaagtt ccgcccgaaa tccccagcga ttactcatgt ggcgccaaat gatctggtgg
     2161 tatgatctcc ggccagaggt acctaccatt gctccgatcg gggccccact gtccgttcat
     2221 cgacgtcgat gcacccttcg ccgggcagtc accggctttg ggcgcggaca ggcagatcgc
     2281 atcgtgccag cgacgactgg ccccaatcat gaggagtcgg actatggcgc gctacttgat
     2341 tggtcgagtc gccggtcttg tctttgttct gttcgctgtg tcgatcatcg cgttcgcctt
     2401 gatgcaggcc gtccccgggg gaccgttcgg cgccaatgag aagggcggct cgatggccgc
     2461 cgaaaccctc aaggtccaga agcacaaata cggcctcgac cagcccgtct acgttcagta
     2521 cgtcaagtac atgtatcacg cggtccaggg tgacttcggc gtgccgttcc agagcccgac
     2581 cgaaaccgtg accggtctca tcgcccgcgc ctggccggcg acgctcaaga tcggcatctg
     2641 cacgatcctc ctcgcctaca gcgtcggcac cctcttcggc ttcctcgccg caaggcacca
     2701 gaatggcatc gtggattatg tcgtgtcatt cacggccacc ctgggcatcg tgctgccctc
     2761 gttcgtcgtg gcgatgtgga tggtgctcat cttcagcatc tatctcaact ggttaccatc
     2821 gggtggctgg ggctcctgga aggagtacat catgcccgtg atcgcctatt cactggcgcc
     2881 gatggcactc gtcgcgagat atacgcgagg c
//
LOCUS       DASTMD010000352         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1462387, whole genome shotgun sequence.
ACCESSION   DASTMD010000352 DASTMD010000000
VERSION     DASTMD010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462387"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1479)
                     /locus_tag="VFQ54_05150"
     CDS             complement(118..1479)
                     /locus_tag="VFQ54_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0164411.1"
                     /translation="MTRFDRRSILKAGAVATGLAAAARFGGSALAQTPGASPAASPAS
                     DLPTSGFDTSSLTVSKPVEIEYWQYQLDTKVDLVNKLIPEFEAANPNIKIKHVNYPYD
                     DFRNQVAAGVQAGAGPDVLNLYYGWIPAYVQQQFLTAFPTDIFPTEQIEKEFYPTVAT
                     GKIQGSYYALPIAVRTLALFYNIDILDAAGVKPPTTWEELVDAAKATVKKNGNKFETV
                     GFTWDIGGQGHNWWRESLIRQNGGVPISDDNKTLDWTSDEAIEAFNYVTAFLTDYDVT
                     QNAFETDGPTAFGAGKAALHVDGSYRVGSLASGSPTLNYGVVPLPGHKSQASFASYWA
                     NSITRNAADGDKLAASAKWIDFLSSESVQKRWTPIVGELPARVSLADDPDLAANPKLK
                     PFIDSLPYSRATFMVNEADLRQFVMDAFDNVTLNGVDPKSAIQEAQDKVQAELDDYWK
                     TFG"
     regulatory      complement(1650..1754)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2112..>2921)
                     /locus_tag="VFQ54_05155"
     CDS             complement(2112..>2921)
                     /locus_tag="VFQ54_05155"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0164412.1"
                     /translation="FDFPDPEREFSTPQVLAERWIAELDKYGLDRIGFVTGRDNDTLA
                     SVVSNYPDRFVGYAHHDITRPDAAEELERAVKVLGLKGLKQLAPMVPILINDRSLYPV
                     WEVCERLGIPVLLHFGMQGAAGGISYHPNMSAAVLEPVARDFPTVDFIVPHFGIQHVT
                     DLLYLCWSCPNVHVDTSGSNQWVRWMPYKLTLEDLFQRFYETIGPERILFGSDSYWFP
                     RGFAIRYLQDQVRICRFMGMSHDELQLIFGGNAARLLDVPLEGVYPVPVST"
BASE COUNT          563 a          892 c          916 g          550 t
ORIGIN      
        1 gtgacgacgg tcggcccggc gactcgcgag cgtccagtga acgcaggagc cgccgggccg
       61 gtgagatggc ggagcatccg aggtgctgcg acgagatgcc ctccacagag aactgatcta
      121 tccgaaggtc ttccagtagt cgtccagctc ggcctggacc ttgtcctggg cctcctggat
      181 cgccgacttc ggatcgacgc cgttgagcgt gacgttgtcg aaggcatcca tcacgaactg
      241 gcgaagatcg gcctcgttga ccatgaacgt cgcgcgcgag tacggcagcg agtcgatgaa
      301 cggcttcagc ttcggattgg ccgcgagatc cggatcatcc gccagcgaga cgcgggccgg
      361 aagctcgccc acgatcggcg tccagcgctt ctgcacggac tcggaggaga ggaagtcgat
      421 ccacttcgcg gatgcggcca gcttgtcgcc atcggcggcg ttgcgggtga tgctgttcgc
      481 ccagtaggag gcgaaactcg cctgcgactt gtgacccggc aacggcacca cgccgtagtt
      541 caacgtgggc gatcccgatg caagcgatcc gacgcggtac gagccgtcga cgtggagtgc
      601 cgccttgccg gcgccgaagg ccgtcgggcc gtccgtctcg aacgcgttct gggtcacgtc
      661 gtagtcggtg aggaaggccg tgacgtagtt gaacgcctcg atcgcctcat ccgacgtcca
      721 gtcgagggtc ttgttgtcgt cggagatcgg cacgccgcca ttctggcgaa tcaggctctc
      781 gcgccaccag ttatggccct ggcccccgat gtcccaagtg aagccgacgg tctcgaactt
      841 gttcccgttc ttcttcactg tcgccttcgc agcgtcgacg agttcttccc aggttgtcgg
      901 cggcttcaca ccggcggcat cgaggatatc gatgttatag aagagggcga gtgtgcggac
      961 ggcgatcggc aatgcgtagt aactaccctg aatcttgccg gtcgcgaccg tcggatagaa
     1021 ttccttctcg atctgttcgg tcgggaaaat atcagtcggg aacgcggtca ggaactgctg
     1081 ctggacgtac gccgggatcc agccatagta gagattgaga acgtccgggc cggcaccggc
     1141 ctgtacgcct gcggcgacct gattgcggaa atcatcgtag gggtaattga catgcttgat
     1201 cttgatgttc gggttggcgg cctcgaactc cgggatcaat ttgttgacga ggtcgacctt
     1261 ggtgtcaagt tgatactgcc agtactcgat ctcgaccggc ttactgacgg tcagactcga
     1321 ggtgtcgaac ccactcgtcg ggaggtcgga cgcgggtgac gcggccgggg acgcgccggg
     1381 tgtctgggcg agggcggagc ccccgaaccg ggcagcggcg gcgagaccgg tcgccacggc
     1441 gccggccttc agaatcgaac gtcgatcgaa tcgtgtcatc ttctcggttc tccctttttc
     1501 gaggagcatc ccgcaccggg atgccgaacg gttacgacga agaccgcgct cccgatccag
     1561 atccgccatg aggccaacaa aagaccccca tccgcttgtc gcgaaagggg gtaccgcaac
     1621 cgcacgaaac gactcgtggg ctccgtacct cctcatcgtc cagcgtgact gctgacggca
     1681 ttagcaccct gccatcgaaa tggtcggttg ctggagcttc acagggccga tccctctgct
     1741 cctctggatg agtcgtacga tcttctcctg accagaaagg tagccgggag gatgtcatac
     1801 gtcaagtcac tttatattcc attcaacacc accggaggaa agccgataca tttcggcaaa
     1861 gttgttcgta acaatttcgg ctattcccgt tcttccgaat cgaacgggcc gaatgtcaac
     1921 acacggtact tgctagcggc ctcattcgcc catcccggcg accccggtgg tggtcgccgg
     1981 gacgggcggg cggcgcatcg caccgacgcg gcggtcgttc gtatggcggg acctccgtgt
     2041 cccgctgccg atgacatcgc cgacctgcca cacatcgcaa cctcacgcaa tcgcttcggg
     2101 caacctctgt ctcacgtcga cactggcacc ggatagacgc cttcgagcgg cacatccagg
     2161 agacgcgcgg cgttgccgcc aaagatcagc tgtagctcgt catggctcat ccccatgaag
     2221 cggcagattc ggacctgatc ctggaggtac cggatcgcaa agccacgagg gaaccagtag
     2281 gagtcgctgc cgaagaggat tcgctcggga ccgatcgtct cgtagaagcg ctggaagagg
     2341 tcctcaagcg tcagcttgta gggcatccag cgcacccact ggttggagcc ggacgtatcg
     2401 acatggacgt tcgggcaaga ccagcaaagg taaagcagat cggtaacatg ctggattccg
     2461 aagtggggca cgatgaaatc gacggtcggg aagtcccgcg cgaccggttc cagcaccgcc
     2521 gctgacatgt tggggtgata cgagatccct cccgccgctc cctgcatccc gaaatgcagc
     2581 aggacgggga tgccaagccg ctcgcagacc tcccagacgg gatagagcga gcgatcgttg
     2641 atcaggatcg gcaccatcgg ggcgagttgc ttgagaccct tcagtcccag caccttgacc
     2701 gcgcgttcga gttcttcggc ggcgtccggc cgggtgatat cgtgatgagc gtaaccaacg
     2761 aaccgatccg ggtagttact gacgaccgac gccagcgtgt cattgtcgcg ccccgtcacg
     2821 aaaccgatcc ggtcaagtcc gtacttgtcg agctcggcga tccaacgctc ggccagaacc
     2881 tgcggcgtcg agaattcgcg ctccggatcg gggaagtcga a
//
LOCUS       DASTMD010000353         3765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1550803, whole genome shotgun sequence.
ACCESSION   DASTMD010000353 DASTMD010000000
VERSION     DASTMD010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550803"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1381)
                     /locus_tag="VFQ54_05160"
     CDS             complement(221..1381)
                     /locus_tag="VFQ54_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0164413.1"
                     /translation="MKITAIKGYPIFVGHRNQFVIKVETDEGISGIGEGGISGRELAM
                     QGMLQHYADLIVGEDPRRIEHIWQLLYRGAYFEGGKISGAVISAIDIALWDILGQSLN
                     VPIWQLLGGAVRDRVPCFATPASLNDPLLLEKAQAALDNGWKILRFGVGMTRDGWTGA
                     DGSVFEPLESINNAVDGLTRLRKELGPDVALSIDFHHRLSAAESAIFCRRIEDLHLYF
                     VEEPMRCEDPNAYAQLRTMTGVPLALGEEFSSKWAFAPYVERGLLNFARVDICNVGGI
                     TEARKVTNWCETHYIDIMPHNPLGPISTAALVHLGSAVTNFAYLEYFAGHYTDAPRDL
                     FPQILEMEGTTFPLPTTPGLGVTFDEAVAADHPYQTWIAPRYHREDGSYTNW"
     gene            complement(1496..2275)
                     /locus_tag="VFQ54_05165"
     CDS             complement(1496..2275)
                     /locus_tag="VFQ54_05165"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU0164414.1"
                     /translation="MTRSENLPVGDEVFADGNMTPVVRRGDEVRRTRSPWWRASAAVL
                     HHLEDVGFTGSPRLLGYDGETERLSFVAGESIDASLDGFLEDTQLDRIGRMIRAYHDA
                     MMTFEMPGDLAWPVMVGAPPSDGTICHNDIAPWNTIVHGDLPIAFIDWDLVAPGTRAW
                     DLAYAAWRFVPLYPDERYGTPRERRRRIGVLLDAHGLSSFDRRGFLDLVRQRIRSAYE
                     TVEVWGRQGVPGFSRLYEGRMHIGALDDLVWLDRSLPGSDV"
     gene            complement(2272..>3765)
                     /locus_tag="VFQ54_05170"
     CDS             complement(2272..>3765)
                     /locus_tag="VFQ54_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU0164415.1"
                     /translation="PTGGAISLAPARTVGYLRQGFADLPEGTLGDLLDIPTRGLFSAQ
                     VQMDASALALGDPGDPDMLADAFDRATAVFEAAGGYAALDELDALLDRFGLGDVALDR
                     LLATLSGGQKTRAGLAGLLASRPDLLVLDEPTNHLDIDALDWLAQFLVRSNSAVLMVS
                     HDRGFLDQVATDIFELDGITHRLTIYPGNYSEYAEQKRHAEEEHAAAWARQQKEVRRI
                     KEDIRRMEHHARTIEANTIDYAVRKKAAKIARPAVVRKAKLERMLDSEEAIDRPSQSW
                     GLAMELKGISGGARDVVSLEGVSVAFGGTEVLHGVSLDVQHGDRVALIGPNGSGKSTL
                     IKIIAGLVEPTDGRVRLGTNVNVGYFAQEQDSLDLERTVLETARRGAAMSESDTRTFL
                     HRFLFAGEMVHRKVAELSYGERARLMLALLVLQGTNLLLLDEPLNHLDISSRENFERA
                     LVQFEGTMILVLHDRYAVERLASRVVEIRDGVAVEIDPATIATKVSA"
BASE COUNT          694 a         1188 c         1216 g          667 t
ORIGIN      
        1 gctcgatctt gcaaggaggc tgatggcgac agcgccatca gcctccttgc ttttcgccct
       61 ccgctgcgca cattttgcgc aacatatccc tttggtcatc aaaaacaccg ataaccgctt
      121 tcccaagcga gaggaggaga cggcccgagg gatacacggg cggctccggc ggtgggtggc
      181 atcagagcag aggacgccac gcaccgccag agagtggact ctaccagttc gtgtagctgc
      241 catcctcgcg atggtaccgg ggcgcgatcc aggtctggta cggatgatcg gcggcgaccg
      301 cttcgtcgaa ggtcacgccg aggccgggag tggttggcag cgggaaggtc gtgccttcca
      361 tctccaggat ctgcgggaag agatcgcgcg gggcatccgt atagtgaccg gcgaagtact
      421 cgaggtaggc gaagttcgtg accgccgagc cgagatgcac gagcgccgcc gtcgagatcg
      481 gacccagcgg gttgtggggc atgatgtcga tgtagtgcgt ctcgcaccag ttcgtcacct
      541 tccgcgcctc ggtgatgccg ccgacattgc agatgtcgac gcgcgcgaag ttgagcagcc
      601 cgcgctcgac gtagggtgcg aacgcccact tgctggagaa ctcctcgccg agagcgagcg
      661 gaacccccgt catcgtccgg agctgggcat aggcgttcgg atcctcgcac cgcatcggtt
      721 cctcgacgaa gtagaggtga aggtcctcga tccgcctgca gaagatcgcc gattccgcgg
      781 cgctgaggcg gtggtggaag tcgatcgaga gtgcgacatc gggaccgagt tccttgcgaa
      841 gtcgcgtcag gccatcgacc gcgttgttga tcgattccag cggctcgaag accgacccgt
      901 ccgcgcccgt ccatccatcc cgcgtcatcc cgacgccgaa gcggaggatc ttccagccgt
      961 tgtcgagtgc cgcctgggct ttctcaagca gcagcggatc gttcagggac gctggcgtgg
     1021 cgaagcaggg gacgcggtcg cgcacggcgc cgccaagcag ctgccagatc ggcacgttca
     1081 gcgattggcc gagaatgtcc cagagcgcga tgtcgatcgc cgaaatcacc gcgccggaaa
     1141 tcttgccgcc ctcgaagtag gcgccgcgat agaggagttg ccagatatgc tcgatccggc
     1201 gcggatcctc gccgacgatg agatcggcgt agtgctggag catgccctgc atcgccagtt
     1261 cgcggccgga gatgccgccc tcgccgatgc cggagatacc ctcgtccgtc tcgaccttga
     1321 tgacgaactg gttccggtga cccacaaaga tcgggtagcc cttgatcgcc gtgatcttca
     1381 cgcttcactc ctcctattgg tttatgagag ttcgctggag gtgatatagc gtggtggagg
     1441 atggtgggca attggttgaa atgaccaaag ggatatgtcg catggaagac ttcttctaca
     1501 catccgatcc cggaagactt cgatcgagcc agacgaggtc gtcaagcgca ccgatatgca
     1561 ttcgaccttc atagagacgg gagaagcccg ggacaccctg acgcccccag acttccacgg
     1621 tctcgtaggc cgatcgaatt cgctgccgga cgagatcgag gaacccccgg cgatcgaatg
     1681 acgacaggcc gtgggcatcg agcaggacgc cgatccgccg ccggcgctcg cgcggggttc
     1741 cgtaccgttc atccggatag agcggcacga agcgccaggc cgcataggcg agatcccacg
     1801 cgcgcgtccc tggcgcgacg agatcccagt cgatgaaggc gatgggcaga tcgccgtgca
     1861 cgatcgtatt ccacggcgcg atgtcgttgt gacagatcgt gccatcactc gggggtgcac
     1921 cgaccatcac cggccaggca agatcgcccg gcatctcgaa ggtcatcatc gcgtcatgat
     1981 aggcgcggat catccttccg atccggtcga gctgggtatc ttcaaggaac ccgtcgagcg
     2041 aggcatcgat gctctcgccg gcgacgaacg agagccgctc ggtctcgccg tcgtatccca
     2101 ggagtcgcgg cgaaccggtg aagcccacat cctcgagatg gtgcagcacg gccgcgctcg
     2161 cccgccacca cggagagcgg gtgcgacgga cttcgtcgcc gcgccgaacc acgggcgtca
     2221 tgttgccatc agcgaagacc tcgtcgccca ccggcaggtt ctcggatctg gtcatgccga
     2281 aaccttcgtc gcgattgtcg ccggatcgat ctcgaccgcg acgccgtccc gaatttcgac
     2341 cacgcgactt gcgagccgtt ccaccgcata gcgatcgtgg aggaccagga tcatcgtgcc
     2401 ttcgaactgc acgagggcac gctcgaaatt ctcccgggat gagatgtcga gatgattgag
     2461 tggctcatcg agcagcagga gattcgttcc ctgcagcacc agcagggcca gcatcaaccg
     2521 cgcgcgttcg ccgtaggaga gctcggcgac cttcctgtgg accatctccc cggcgaagag
     2581 gaagcgatgg aggaaggtcc gcgtgtcgct ttcggacatc gccgccccgc ggcgggcggt
     2641 ctcgagcacc gttcgttcga gatcgagaga atcctgctcc tgcgcgaaat aaccgacatt
     2701 gacgttcgtt cccagcctca cgcgtccatc tgttggttcg acgaggccag cgatgatctt
     2761 gatcaacgtc gacttgccgc tcccgttcgg gccgataagg gcgacgcgat caccgtgctg
     2821 gacatcgagc gacacgccat gcagaacctc ggtgccgcca aaggccaccg acacgccctc
     2881 gagcgagacg acgtctctcg caccgccact gattcccttc agttccatcg ccagtcccca
     2941 cgattgggag ggtcgatcga tcgcttcctc ggagtcgagc atccgctcca gcttcgcctt
     3001 gcgcacgacg gcgggtcgcg cgatcttcgc tgccttcttc ctgacggcgt agtcgatcgt
     3061 gttcgcctcg atcgttcgcg cgtggtgttc catccggcgg atatcttcct tgatccgccg
     3121 tacttccttt tgctggcgcg cccaggcggc ggcatgctct tcctcagcat ggcgcttctg
     3181 ctcggcatac tcggagtaat tgccgggata gatcgtcagg cgatgggtga tgccgtcgag
     3241 ctcgaagatg tcggtggcta cctggtcgag gaagccccgg tcgtgggaca ccatcaacac
     3301 ggcgctattc gaccgcacga ggaactgggc gagccagtcg agggcatcga tgtcgaggtg
     3361 attcgtcggc tcgtcgagaa ccagcagatc ggggcgtgac gcgaggagcc cggcgagacc
     3421 cgcacgcgtc ttctggccac cggagagcgt cgcgagcaga cgatcgaggg cgacgtcccc
     3481 gagtccgaag cggtcgagaa gagcgtcgag ttcatcgagc gcggcgtagc ctccagcggc
     3541 ttcgaagacc gccgtggctc ggtcgaatgc gtcggcaagc atgtcgggat cgccgggatc
     3601 gccaagcgcc agcgcgcttg catccatttg cacctgggcc gagaagaggc cgcgcgtcgg
     3661 gatatcgagc agatcgccga gcgtcccctc gggaagatcg gcgaacccct gccgcaggta
     3721 cccgacggtc cgggccgggg cgagcgagat cgcgccgccg gtggg
//
LOCUS       DASTMD010000354         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1907738, whole genome shotgun sequence.
ACCESSION   DASTMD010000354 DASTMD010000000
VERSION     DASTMD010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907738"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFQ54_05175"
     CDS             complement(<1..556)
                     /locus_tag="VFQ54_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0164416.1"
                     /translation="MTVPLAISGIDATIVHTPYSSTLEPAWSPGTIWRESAATIYRVH
                     TDAGITGIGAARGNPDVVANVVGPLLTGVDPARIEPIVRSIINAAGGFAAIPVVAGIE
                     MALWDLMGKAAGLPLYRLWGAHGSGVRAYASMVEVQTPERRAEDARRLLERGYTAIKL
                     RLHDETMRQDIAQVEAVRDAVGDRM"
     gene            complement(673..1869)
                     /gene="queG"
                     /locus_tag="VFQ54_05180"
     CDS             complement(673..1869)
                     /gene="queG"
                     /locus_tag="VFQ54_05180"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEU0164417.1"
                     /translation="MPDSPPIPNLTLRPIDPRQALTSHVKFLAAKSGLAVSAITSADP
                     FPETARFLDRHIKDGHLAGLDWFTEERANVSADVRNLQPTARSIVSVGLNYWPGTTEM
                     PDDGVKRGRISRYAWGRDYHRVIKRRLKRFHVELEEHVGHPVDVRLLVDTARLVERAV
                     AARSGLGWYGKNACIISPGFGSWILLGEVVVDLDLVPDAPLGKNCGRCTICIDKCPTG
                     AIVAPYTIDTPTCISYLTIEHRGVIARELRPKMGDWVYGCDVCQEVCPYTGAATAADD
                     PELVPASLNNSYPSLHWLLGMSDEDFAATYFGTPVPRTKRFGLARNAAIALGNVGTED
                     DLPVLCRALEEHDHAIVRGHAAWAVGRVGGGVAKAALDRLRRSEPDEWVREEIEYALE
                     IPARTA"
BASE COUNT          346 a          653 c          740 g          339 t
ORIGIN      
        1 tcatccggtc gccgacggcg tcacgaacgg cctcgacctg cgcgatgtcc tggcgcattg
       61 tctcgtcgtg gagacgcagc ttgatcgcgg tgtagccccg ttcgaggagc cgccgcgcat
      121 cttcggcgcg acgttccggc gtctggacct cgaccatgct cgcgtaggcc cgcacgccac
      181 tgccgtgcgc gccccataac cggtagagcg ggagcccggc agccttcccc atcagatccc
      241 agagcgccat ttcgatccct gccacgaccg ggatcgcggc gaagccaccg gcggcattga
      301 tgatggaccg cacgatcggt tcgatgcgcg cgggatcgac acccgtgagg agcggtccga
      361 cgacgttcgc cacgacatcc ggattgccgc gcgcggcgcc gatcccggtg atgccggcat
      421 cggtgtgcac gcgatagatg gtcgcggcgg attcgcgcca gatcgtgccg ggcgaccacg
      481 ccggttcgag cgtgctgctg taaggcgtgt ggacgatggt ggcgtcaatg ccgctgatcg
      541 ccaggggaac agtcatggaa gcgcctccag aacggctgcc ggacggaaga tgtccgggag
      601 ggtgagacag gtcggggatc ggtgtggcgg catcgtcgcg cgaagcggcg aatcgggcaa
      661 gggtggatcc ggtcaggcgg tacgagcagg gatctccagc gcgtattcga tctcctcgcg
      721 cacccattcg tccggctccg atctccggag gcgatccagc gccgccttcg ccacgccgcc
      781 gccgacgcgt ccgaccgccc aggcggcgtg accgcggacg atcgcgtgat cgtgctcctc
      841 cagcgcgcgg cagagtaccg ggaggtcgtc ttccgtgccg acattgccga gggcgatcgc
      901 ggcgttccgg gcgaggccga atcgcttggt ccgtggcacc ggagtaccga agtacgtagc
      961 ggcgaaatcc tcgtcggaca tgcccaacag ccaatggagc gagggatagc tgttgttgag
     1021 tgacgcgggc accaactcgg gatcgtcggc ggcggtcgcg gcgccggtgt agggacagac
     1081 ctcctggcag acatcgcagc cgtagaccca gtcacccatc ttcggtcgca actcgcgggc
     1141 aatgacgccg cgatgctcga tcgtcaggta ggagatgcag gtcggcgtgt cgatcgtgta
     1201 cggcgcgacg atcgccccgg tcggacactt gtcgatgcag atcgtgcagc gaccgcagtt
     1261 cttgccgagc ggcgcatccg gcacgagatc gagatcgacc acgacttcgc cgagcaggat
     1321 ccacgagccg aaacccggcg agatgatgca ggcgttcttc ccgtaccaac caagcccgga
     1381 gcgtgccgcg acggcgcgtt cgacgagccg ggccgtgtcg acgaggaggc gcacatcgac
     1441 gggatgcccg acgtgctctt caagctcgac gtggaagcgt ttcagccggc gcttgatcac
     1501 gcggtggtag tcgcggcccc aggcgtatcg ggagatgcgc cctcgcttga caccgtcatc
     1561 aggcatctcg gtcgttcccg gccagtagtt gagcccgacc gagacgatcg accgcgctgt
     1621 cggctggaga ttgcgaacgt ccgcggatac gttggcgcgt tcctcggtga accagtcgag
     1681 accggcgagg tggccgtcct tgatgtgccg atcgaggaag cgcgcggttt ccgggaacgg
     1741 atcggcggat gtgatcgccg acacggcaag tcccgacttc gcggcaagga acttcacgtg
     1801 tgaggtgagg gcctgacgtg gatcgatcgg gcggagcgtg aggttcggga tgggagggga
     1861 atcaggcacg tccatgcctc gagtataggc cgtcgcgggc gatcagattt gacgcagcgc
     1921 gttcgcaatc tcgtcccagt cgtcgcggcg ggccatgccg agcgagatcg tggcgatgat
     1981 tgtcgcacgg tcgcgagagc cggggggggc gattgcccgg atgggatcgc cggttgtcgc
     2041 gacgacgatg gcgccatcct cggcgtgcat caccgccg
//
LOCUS       DASTMD010000355         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1550837, whole genome shotgun sequence.
ACCESSION   DASTMD010000355 DASTMD010000000
VERSION     DASTMD010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550837"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VFQ54_05185"
     CDS             <1..1181
                     /locus_tag="VFQ54_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0164418.1"
                     /translation="DGKWIDYPLKMQSALKAIGPVTAARAVADYGRAQAQHKIRPAEI
                     KSMKDAYVLQYGYTLYSLFFDGYSQKVWGMSPEKLSADWVSQRTKGMSLMTAVKDAIV
                     PSKGEVVSLIDEFMYPRDGFGRFSDRMADRIIESGNEIRLKTAVEKIVLDGNRVKGIQ
                     VRTENGSEFIEGKNFVSSIPLTVLCKITDPPAPVDVIAAADSLTFRDIITVNLMFKKR
                     QVTNDTWVYVHDRNILFGRFHEPKNWSPAMVPSDDFTSLVVEYFCTKGDHIWSMTDEQ
                     LVNQTVKHLVEDLKFVEPQEVIGGFTVRATKAYPTYDLGYKEPLERMKNYIASIENLQ
                     YIGRGGSFRYNNTDHSIETGLLAAKNLLGEHHNLEKVNTDGEYHEIKRVAQPASQAIH
                     "
     gene            1285..1716
                     /locus_tag="VFQ54_05190"
     CDS             1285..1716
                     /locus_tag="VFQ54_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164419.1"
                     /translation="MSENQETSPTSVPELSGEPAERAVAQPRQKTRVDGIIVWALVSI
                     GFLGYLTLGSISTRSWQLNSFQQAWVLDISPRRILHIWTYQLDHLPNVAHQWALKAVF
                     GGALGIMVVGAVICVWLMLDEVGTGMGSRTEHTDTRAGDSA"
     gene            1801..>2587
                     /locus_tag="VFQ54_05195"
     CDS             1801..>2587
                     /locus_tag="VFQ54_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164420.1"
                     /translation="MIEQSSTAEPRRRVSTTRLWLLAMAVLCVWTAWKLGAFDTMTRF
                     TADGRAFDVPNVFSGVDNPFHATRAYTLLQSLKDGHFLRWIGDHQGGYPAEFYPLGVA
                     WLDVGLWALLFGSLPIVAVHKLAIILIFLLPAVGFWVLARCDKVSPGVAVLATAIQVA
                     VPGGNGLTSWATGGYAELVQWGLVTNVAGGTAAMICCAMLVRYVAHGERGMAIGAGLF
                     AAGAAYANPRSLFAIAIGPVAILIVTLISRNGVSPKIIVMRMVL"
BASE COUNT          479 a          823 c          801 g          484 t
ORIGIN      
        1 tcgacgggaa gtggatcgat tacccgttga agatgcagag tgccctcaag gcgatcggcc
       61 ctgtcactgc cgcccgcgcg gttgccgact acggtcgcgc ccaggcccag cacaagattc
      121 gcccggccga gatcaagtcg atgaaggacg cctacgtcct ccagtatggc tacacgctgt
      181 acagcctctt cttcgacggc tattcccaga aggtctgggg catgtcgccc gagaagctga
      241 gcgccgattg ggtcagccag cgcaccaagg gcatgtcgct gatgactgcc gtcaaggacg
      301 cgatcgtccc ctccaagggt gaagtcgtca gcctcatcga cgaattcatg tacccgcgtg
      361 acgggtttgg acgcttctcc gaccgcatgg cggacaggat catcgagagc ggcaacgaga
      421 ttcgcctgaa gaccgcggtc gagaagatcg tgctcgatgg caaccgcgtg aagggcatcc
      481 aggtccgtac cgagaacggc tcggagttca tcgagggcaa gaactttgtc tcctcgatcc
      541 cgctcaccgt cctctgcaag atcaccgatc cgccggcccc ggtcgatgtc atcgccgccg
      601 ccgattcgct gaccttccgc gacatcatca ccgtcaacct gatgttcaag aagcgccagg
      661 tcacgaacga cacctgggtc tacgtgcatg accgcaacat cctcttcgga cgcttccacg
      721 agccgaagaa ctggagtccg gcgatggttc cgagcgacga cttcacgtcg ctggtggtcg
      781 agtacttctg cacgaagggc gaccatatct ggagcatgac cgacgagcag ctcgtcaacc
      841 agacggtcaa gcacctggtc gaggacctca agttcgtcga gccacaggaa gtgatcggcg
      901 gcttcacggt ccgtgcgacg aaggcgtacc cgacctacga tctcgggtac aaggaaccgc
      961 tggagaggat gaagaactac atcgccagca tcgagaatct ccagtatatc ggtcgcggtg
     1021 gctcgttccg ctacaacaac accgaccatt cgatcgaaac gggcctgctg gcggcgaaga
     1081 acctcctcgg cgagcaccac aacctcgaga aggtgaacac ggacggcgag taccacgaga
     1141 tcaagcgcgt cgcccagccg gcgtcgcagg cgatccacta gtcgcgtatg gccggggcga
     1201 acgcgtcctt cgttgcgttt gcccggtatc acgtcgttac agccgaatcc ggatgtgatg
     1261 acgaacgggc ggaggtcagt ccgcgtgagc gaaaaccagg aaacatcccc cacgagtgtc
     1321 ccggagcttt cgggtgaacc ggctgaacgg gcggtggccc aaccgcgcca gaagacccgg
     1381 gtcgacggca tcatcgtctg ggcgctcgtt tcgatcggct tccttgggta cctgaccctc
     1441 ggttcgatct ccacccgctc ctggcaactc aattccttcc agcaagcctg ggtcctggac
     1501 atttcgccgc gacggatcct tcacatctgg acctaccagc tcgaccatct ccccaatgtc
     1561 gctcaccaat gggcgctcaa ggcggttttc gggggcgcgc tcggaatcat ggtcgttggc
     1621 gccgtgatct gtgtctggct gatgctggac gaggtgggaa ccggcatggg atcacgcacc
     1681 gagcatacgg atacgcgcgc gggggactct gcgtgacgtc ttccccggcc ccgctgaaac
     1741 gaattggttg gcagggatcg acgcgtatct cgcgcgagat cagagacatc ggacgattcg
     1801 ttgattgagc aatcctcgac ggcagaaccg cgccgccgcg tcagcaccac ccggctgtgg
     1861 ttgctggcga tggcggtgtt gtgtgtctgg accgcgtgga agctcggcgc attcgacacg
     1921 atgacccggt tcacggccga cggccgggca ttcgatgtcc cgaatgtctt ttccggtgtc
     1981 gacaatccct tccatgcgac ccgcgcctat acgctgctgc agagcctcaa ggatggacac
     2041 ttcctccgct ggatcggcga ccatcagggt ggctatcccg ccgagttcta tccgctcggc
     2101 gtcgcctggc tcgatgtcgg gctctgggcg ctcctcttcg gctcattgcc gatcgtcgcc
     2161 gtccacaagc ttgcgatcat cctgatcttc ctgcttcccg cggtcggctt ctgggtgctg
     2221 gcgcgatgtg acaaagtcag cccgggcgtt gcggtgctcg caacggcgat ccaggtcgcc
     2281 gtgccgggcg gcaacgggct gacgagctgg gctaccggcg gatatgcgga actcgtccag
     2341 tggggactcg tgaccaatgt cgccggcggg acggcggcaa tgatctgctg cgcgatgctc
     2401 gtgcgctacg tcgcccatgg cgagcgaggc atggcgatcg gggccggact cttcgccgcg
     2461 ggggctgcct atgccaaccc gcggtccctg ttcgcgatcg cgatcggccc cgtggcgatc
     2521 ctcatcgtga cgctgatcag ccgcaacggg gtctcgccga agatcatcgt gatgcgaatg
     2581 gtcctgc
//
LOCUS       DASTMD010000356         6402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1641502, whole genome shotgun sequence.
ACCESSION   DASTMD010000356 DASTMD010000000
VERSION     DASTMD010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641502"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1125
                     /gene="ruvB"
                     /locus_tag="VFQ54_05200"
     CDS             31..1125
                     /gene="ruvB"
                     /locus_tag="VFQ54_05200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012914214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU0164421.1"
                     /translation="MSSEPGPSGPTHTTSAERDASRLVTARQRDEDGGVERSLRPRQL
                     EDYIGQDRVKNNLKIFIQAALERGESLDHVLLYGPPGLGKTTLAAIIASEMGVNLRMT
                     SGPAIERQGDLVSILTNLSPGDVLFIDEIHRLNRVIEEVLYPAMEDFAVDIIIGKGPA
                     ARTMRINLPRFTLVGATTRLALLTGPLRDRFGAVMRLDFYDQDAMERIIRRSASILDF
                     PIDDSGASALAARSRGTPRIANRLLRRVRDVAQVAGETTVSAPIARHALDLLEVDELG
                     LDDTDRRILHAIIDKFGGGPVGIDTLAASTSEETDTIMDVYEPYLIQLGFLQRTPRGR
                     IATKRAYEHMGIPYPVSLSAQQALFQVEEA"
     gene            1122..2195
                     /gene="queA"
                     /locus_tag="VFQ54_05205"
     CDS             1122..2195
                     /gene="queA"
                     /locus_tag="VFQ54_05205"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU0164422.1"
                     /translation="MRSSQTRHLHMSDFDYDLPPELIAQEPLRDRDRSRLLVLDRVTG
                     EFAHRTFEALPDLLDPGDLIVVNDSRVIPARLIGRRESGGEVEVLLLRREEASGQWRA
                     LARPTKRLRIGERMHFVPKDDPRGPEAGITLVEKLDDGQVIVELEPVLGDNLDAYGRV
                     PLPPYITHSLADDERYQTVYSAHKGSAAAPTAGLHFTPEILERLAARAIERASVTLHV
                     GLDTFRPVSEEFAEDHVIHQEWCSVPEDAWEKIHATRERGSRVVAIGTTAARTLETLG
                     QRLARGETGAFSAMTNIYITPGFKWQVVDAMVTNFHLPKSTLLLMISSFAGRDHVLAA
                     YEAAIAERYRFFSFGDAMLIREG"
     gene            2331..2948
                     /locus_tag="VFQ54_05210"
     CDS             2331..2948
                     /locus_tag="VFQ54_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEU0164423.1"
                     /translation="MSDYPDLNGKTRDETWVLLNEWVKSPSLIRHCLSVETAVRAYAG
                     KFGEPEEAWSVVGLIHDFDYEKHPTLDEHPMVGAGVLKDLGYPQWVVDAVLSHADLPQ
                     YPRRTLLEKTLFSVDELTGFISAVALVRPSKAVADVQVSSVKKKMKDKAFAAAVRRED
                     LTKGAEELGISLDEHIAFVIQAMTENAEALGLAGVGAATVASDQG"
     gene            3020..3547
                     /locus_tag="VFQ54_05215"
     CDS             3020..3547
                     /locus_tag="VFQ54_05215"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0164424.1"
                     /translation="MAKQWNKPFDNQLDAEKTYTGTIETSHGTIEVEFYPEDAPITVN
                     NFVSLAREGYYDGTPFHRIVKGFVIQGGDPTGTGTGGPGFKFQDEKITKDYEPGTLAM
                     ANAGPNTNGSQFFIVLRDLKGSLPKNYTIFGKVTSGFDVAQKIEAVPTTYGRSGEPST
                     PTEPVTLNKVTITEA"
     gene            complement(3627..3869)
                     /locus_tag="VFQ54_05220"
     CDS             complement(3627..3869)
                     /locus_tag="VFQ54_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164425.1"
                     /translation="MSVYAPVQPIPRAGAEFEQQQALLAKLEQRLDDGYARIELGIAR
                     GVNVEHWEEFWLELLHQYERLVDEFAASRQILAAAA"
     gene            complement(3941..4555)
                     /locus_tag="VFQ54_05225"
     CDS             complement(3941..4555)
                     /locus_tag="VFQ54_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164426.1"
                     /translation="MEHVAFDPSRYLTKVKGNDYLEVKWRLVWLRDQYPDAVIETDLI
                     SHVGNQAIFRAKVTLPSGASSTGWGSEEISGFVNYVEKAETKAIGRALAALGFGTQFC
                     PDFDYGADAGQVVDAPVDRQSFQQPTALFVSREETQDHAPSPKQAGFIQAISRELRMN
                     AQGLNEFTQRLVGKDADQLSRRDASAVIEALKEQQQTRSSQRAS"
     gene            4783..5925
                     /gene="dnaN"
                     /locus_tag="VFQ54_05230"
     CDS             4783..5925
                     /gene="dnaN"
                     /locus_tag="VFQ54_05230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU0164427.1"
                     /translation="MKVACLQENLQRGLGRVARAVASRTALPVLSNVLIATDEGRIRI
                     AATNLEIGVTTWIEGNIEEEGRITVDARLLAEFVNTLPPGQVSLTVDPTRFSLTVQSG
                     RDRAAINGIDAEDFPIMPTISDEGFSLEIDPQRLRESINLIEFAAATDESRPILAGVL
                     TRFDGDTLMFASSDGFRMAVNESPLDQPVAERMDIIVPARAYRELSRIIGDLDEPIRL
                     AVTPNRSQVMARLGDTEWVSRLIDGTFPDIKQIVPKEFASRIDLSREALLNAVRRAGY
                     FARENNDVVRFSVQPGEDDLSPGSVEVSANAAERGNSQSYVDASVSGPAIQIAFNGRY
                     LIDVLSVLKHGQVTFGFNGTNQAGVIRPSGNDAYSHVIMPMVIGGA"
     gene            6023..>6402
                     /locus_tag="VFQ54_05235"
     CDS             6023..>6402
                     /locus_tag="VFQ54_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0164428.1"
                     /translation="MYLSRLHLEEFRNYRTLELNLDPIGLRLIGQNGSGKTSLLESIV
                     MLATTRSPRASTDRELVEWESGEIYGVAPYARIEATVTSVERTHELAVTIELDREGGG
                     VAKKGFILDGKPVRAHDLIGVIKFV"
BASE COUNT         1209 a         1975 c         2037 g         1181 t
ORIGIN      
        1 gatgttcttc tcaggcgtta tactaggcgc atgagtagcg agccgggtcc gtcgggacct
       61 acccacacca cttcagctga acgggatgcc tcacggctcg tcaccgcgcg ccagcgcgac
      121 gaggacggtg gcgtcgagcg atccttgcga ccgcgccagc ttgaggacta catcggccag
      181 gatcgcgtca agaacaacct gaagatcttc atccaggctg cgctcgaacg cggagagtcg
      241 ctcgatcacg tgctgctcta cggaccgccg ggactcggca agaccacact cgccgcgatc
      301 atcgcctcgg agatgggggt caaccttcgg atgacgtccg gtcccgcgat cgagcgccag
      361 ggcgatctcg tttcgatcct gacgaatctc tcgccgggcg acgtgctctt catcgatgag
      421 atccaccgcc tgaaccgggt aatcgaggaa gtcctctatc cggcgatgga agatttcgcg
      481 gtggacatca tcatcggcaa ggggcccgcg gcgcgaacga tgcggatcaa tctcccccgg
      541 ttcacgctcg tcggggcgac gacgcggctg gcgttgctga ccggtccgct ccgcgatcgg
      601 ttcggcgccg tgatgcgtct tgatttctac gaccaggatg cgatggagcg gatcatccgg
      661 cgctcggcct cgattctcga ttttccgatc gatgacagcg gcgcctcggc gctggccgcg
      721 cgttctcgcg gcacgccgcg catcgcgaac cgcctcctcc gccgcgtgcg tgacgtggcg
      781 caggtcgccg gtgagacgac cgtctcggca ccgatcgccc gtcacgcgct cgatcttctc
      841 gaagtcgatg agctcgggct cgacgatacc gaccgccgga tcctgcacgc catcatcgac
      901 aagtttggtg gcgggccggt ggggatcgat acgctggcgg cgtccacgag cgaagagacc
      961 gatacgatca tggatgtcta cgagccctat ctgatccagc tcggtttcct gcagcggacg
     1021 ccccgcggtc gaatcgcgac caagcgcgcg tacgagcaca tggggattcc ctatccggtg
     1081 tcgctctcgg cgcagcaggc actctttcag gtcgaggaag catgaggtcg agtcagacgc
     1141 gccaccttca catgagcgac ttcgactacg atttgccgcc tgagctcatc gcccaggaac
     1201 cgctccgcga ccgcgatcgg agccggcttc tggtgctcga tcgcgtgacc ggtgagttcg
     1261 cgcatcggac cttcgaggcc ctccccgatc ttctcgatcc cggcgacctg atcgtcgtca
     1321 acgacagccg cgtgattccc gcccgcctga tcggacgccg ggagtcgggt ggcgaggtcg
     1381 aagtcctgct gctgaggcgg gaggaagcga gtggccaatg gcgagccctc gcgcggccga
     1441 cgaagcgact gcgaatcggc gaacgcatgc atttcgttcc gaaggacgat cctcggggtc
     1501 cggaagccgg catcaccctg gtcgagaagc tggacgacgg ccaggtgatc gtcgaactcg
     1561 agccggtgct cggggacaat ctcgacgcat atggtcgcgt gccgctgccc ccgtacatca
     1621 cgcattcgct tgccgacgat gagcggtatc agacggtcta ctccgcacac aagggctccg
     1681 ccgccgcgcc gacggccgga ctccatttca caccggagat cctcgaacga ctggccgcgc
     1741 gggccatcga gcgggcctcg gtcacgctgc atgttggtct cgacacgttc cgccccgtct
     1801 ccgaggaatt cgccgaggat cacgtcattc accaggaatg gtgttccgta ccggaggacg
     1861 cgtgggagaa gatccatgcg accagggaac gtgggtctcg tgtcgtcgcg atcgggacga
     1921 ccgcggcgcg gacactggaa acgctgggac agcgtctcgc gcgcggggag accggggcat
     1981 tttcggcgat gacgaacatc tacatcacac cgggcttcaa gtggcaggtg gttgatgcga
     2041 tggtgaccaa cttccacttg ccgaaatcga cattgttgct gatgatcagc tcgttcgcgg
     2101 gccgcgatca cgttctcgcc gcctatgagg ccgcgattgc ggagcgatat cgcttcttct
     2161 cgttcggtga tgcgatgctg atccgcgagg ggtgagcact cgttcccttc ctgccgcccg
     2221 ccctcaggat cgcgagacca aaggttgacg taacgacagt cgacggtatc gttcccgtac
     2281 ctgcgcgaca catggtcgcc gaagttcact tcgttcagga gatcttttcg atgagtgact
     2341 atccggatct gaatggcaag acgcgcgacg agacatgggt cctcctcaac gaatgggtca
     2401 agagtccgag cctgatccgc cactgcctca gcgtggagac ggcggtccgg gcttacgccg
     2461 gcaagttcgg cgagccggag gaggcgtggt cggtcgtcgg gctgattcac gatttcgact
     2521 atgaaaagca tccgaccctc gatgagcatc cgatggttgg cgccggggtt ctgaaggatc
     2581 tcggctatcc gcagtgggtc gtcgatgccg tgctcagtca tgccgatctt ccccagtatc
     2641 cgcgtcggac gcttctcgag aagacactct tctccgtcga cgaattgacc ggcttcatct
     2701 ccgcggtcgc gctggttcgc ccgtcgaagg ccgttgccga tgtccaggtc tcgtcggtca
     2761 agaagaagat gaaggacaag gcatttgccg cggcggtgcg gcgggaagac ctcacgaaag
     2821 gcgccgaaga actcggcatc tcgctcgacg agcacatcgc attcgtcatc caggcgatga
     2881 ccgagaacgc ggaggcgctg ggtctcgcag gggtgggcgc cgcgactgtc gcgagcgatc
     2941 aggggtagac tggttcgcga cacacggtat tgacacaggt gagtaccgcg gacgaattgc
     3001 agaagggatt tgtttttcga tggcgaagca gtggaacaag ccgtttgaca accagctcga
     3061 tgccgagaag acgtacaccg gcacgatcga gacgagccac ggcacgatcg aggttgagtt
     3121 ttacccggaa gacgcaccga tcacggtcaa caacttcgtg agcctcgctc gcgaggggta
     3181 ctacgacgga acgccgttcc atcggatcgt caagggcttc gtgatccagg gtggcgaccc
     3241 gaccgggacg ggaacgggtg gtccgggctt caagttccag gacgagaaga tcaccaagga
     3301 ctacgagccg ggcacgctcg cgatggcgaa tgcggggccg aacaccaacg gcagccagtt
     3361 cttcatcgtg ctccgcgacc tgaaagggag cctcccgaag aactacacga tcttcggcaa
     3421 ggtgacctcc ggctttgacg tcgcgcagaa gatcgaggcg gtcccgacga cctacggtcg
     3481 cagtggcgag ccgtccacgc cgaccgagcc ggtgacgttg aacaaggtga cgatcaccga
     3541 ggcgtaagcc tcgacggtcg gacgcgaaac gagggctggc gcatcatcgc gccagccctc
     3601 gtcttgtctt gatcggggga tactcgttac gccgccgccg cgagaatctg ccgggaagcg
     3661 gcgaactcat ccacgagtcg ttcgtactga tggaggagtt caagccagaa ctcttcccag
     3721 tgctcgacat tcacgcctcg tgcgatgccc agctcgatcc gggcatatcc atcatcgaga
     3781 cgttgttcga gcttggcgag cagcgcctgt tgctgctcga attcggcccc cgcgcgcggg
     3841 atcggttgaa ctggtgcgta aacagacatc gagatggtcc ttcccatgat ccggaagtcg
     3901 gctgttcggg atcgccgcca tcctcggctc acccacggaa ctatgaggcg cgctgtgacg
     3961 aacgcgtctg ctgctgttcc ttgagtgcct cgatcaccgc tgatgcgtcg cggcgggaga
     4021 gctgatcggc atccttgccg acgagccgct gtgtgaactc attcagcccc tgggcgttca
     4081 tccgcaactc gcggctgata gcctggatga agcctgcctg cttcggagac ggcgcatgat
     4141 cctgcgtctc ctcgcggctt acgaagagcg ccgtcggctg ctgaaagctc tggcgatcga
     4201 ccggggcatc gacgacctgg ccggcatcgg ccccatagtc gaagtcaggg cagaactggg
     4261 tgccaaagcc gagcgccgcg agcgcgcggc cgatcgcctt ggtctcggcc ttctcgacgt
     4321 agttgacgaa gccactgatc tcttcgctac cccaaccggt cgatgaagct cccgatggga
     4381 gtgttacctt tgcgcggaag atcgcctggt tgccgacgtg ggaaatcagg tcggtctcga
     4441 tcacggcatc cggatactga tcgcggagcc agacgaggcg ccacttgacc tccagataat
     4501 cgtttccctt gaccttggtc agatatcggc ttggatcgaa tgcgacgtgt tccatgaatg
     4561 ccctccaggc catcgaacga atgttctgtt cgttgttctc attttagaac agatgatctg
     4621 tttggtcaac caggcacagc aaggactctg cacagggata ttccaaccgg cgcgagcgcc
     4681 atgaggaccg ggcgtatgca tgaatagggg gacgggcgtc cccgcgtggt acaatcctgc
     4741 ttgttttgtc cgtacgtacg gtcacgtgaa cgggagcatg gggtgaaggt cgcttgcctt
     4801 caggagaatc ttcaacgggg actcgggcga gtcgcgcgag ctgtcgcgtc cagaaccgcc
     4861 ctgcctgttc tcagtaatgt cctgatcgcc accgatgagg ggcgcatccg gatcgcggca
     4921 acaaatctcg agatcggggt cacgacctgg atcgaaggca atatcgagga agaaggacgg
     4981 atcacggtcg atgcccgtct gctcgcagaa ttcgtgaata cgcttccacc aggtcaggtc
     5041 agcctcacgg tcgatccaac ccggttctcg ctcaccgtgc agagcggtcg ggatcgcgcc
     5101 gcgatcaacg ggatcgatgc cgaggacttt ccgatcatgc cgacaatctc ggacgaggga
     5161 ttctcgctcg agatcgatcc gcaacgactc cgcgagtcga tcaatctgat cgagttcgcc
     5221 gccgcgaccg acgagagccg gccgatcctg gccggcgtcc tgacgcgatt cgacggcgac
     5281 accctgatgt tcgcctcttc cgacggtttc cggatggcgg tgaacgagag cccgctcgat
     5341 cagccggtgg cggaacggat ggatatcatc gttcccgccc gcgcctatcg cgaactctca
     5401 cgcatcatcg gcgatctcga tgaaccgatt cgccttgccg tgacgccgaa tcgcagccag
     5461 gtgatggcgc ggctcggtga taccgagtgg gtatcgcgac tgatcgacgg gactttcccc
     5521 gacatcaagc agatcgttcc gaaggagttc gcgagcagga tcgacctgag tcgggaggcg
     5581 ctcctgaacg ccgtgcggcg ggcgggatac ttcgcccgcg agaacaacga cgtggtgcga
     5641 ttctccgtgc agccgggcga ggacgatctg agtcccggca gcgtggaggt ttccgccaac
     5701 gcggcggagc gcggcaacag tcagagctac gtcgacgcga gtgtctccgg accggcgatc
     5761 cagatcgcgt tcaacgggcg ctacctgatt gatgtactct ccgtgctgaa gcacggtcag
     5821 gtgacgttcg gattcaacgg gacgaaccag gcgggcgtga tccgcccgag cggcaacgat
     5881 gcctactcgc atgtgatcat gccgatggtg atcggcggcg cctgacccca ttctcgagaa
     5941 gacatatccc aggcgaagcg gcagggcgtc gacgatgcct gccgcttcgc cgtttccgca
     6001 cgacaggtct gagatcccac ccatgtacct ttcccgcctg catctggaag agttccgcaa
     6061 ctaccgcacg ctcgagctga acctggaccc gatcgggctg cgactgatcg gtcagaacgg
     6121 cagcggcaag acaagcctgc tcgagtcgat cgtgatgctg gcgacgacgc ggtccccgcg
     6181 cgcctccacg gatcgcgagc tggttgaatg ggaaagcggc gagatctacg gggtcgcgcc
     6241 gtacgcgaga atcgaagcca ccgtgaccag cgtcgagcgg acccacgagc tggccgttac
     6301 gatcgaactc gatcgcgagg gcggcggcgt ggcgaagaag ggattcatcc tcgacggcaa
     6361 gccggtccgc gcgcacgacc tgatcggggt catcaagttc gt
//
LOCUS       DASTMD010000357         1665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1015138, whole genome shotgun sequence.
ACCESSION   DASTMD010000357 DASTMD010000000
VERSION     DASTMD010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015138"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1665
                     /locus_tag="VFQ54_05240"
     CDS             <1..>1665
                     /locus_tag="VFQ54_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU0164429.1"
                     /translation="IGVMFGCCSEPIAFNRRDLCLVSGLAELISHEVMKARVEKARSR
                     HFEVLITQVQESLAIFSVDGRMLYESPAAREMLGYPDGVIESMTPDELLALIHPDDLA
                     GTLEVRRELLANPNASRSFLHRVRHVDGSWRTIRVAASNLLDEPSVGGLVFTSRDITE
                     QNAHDEQLQYEARHDSLTGLLNRATIERHLHGLLADPRVPVAFVYIDIDDFKLVNDTF
                     GHFAGDEVLRVVSRRMDAFVREGELVGRFGGDEFVMLIPDVSYECAQGRAEELADVLQ
                     RPMRLQGSDVVAICSIGVARATEHGTDLDTLFRAADAALYRVKSSGGGSVAMYATSLD
                     NAIHERHAIDHEFTLALERDELVLEYQSIVELESGEVIGMEALVRWNHPTRGRLMPMS
                     FVPILEELGIAGRLGRWVLDRAARQVKRGGVSVNVNVSPQHLREPTFMRDVLAAIETH
                     GIDASALVLEITETASILHDSVGMTVLSRLKDLGVTFAIDDFGTGYSGLSALRNYPVQ
                     MMKLDRVFLERLESDLRDQEIVRAVINVAKALGIAVVVEGIESMTQA"
BASE COUNT          290 a          506 c          550 g          319 t
ORIGIN      
        1 attggcgtga tgttcggctg ttgcagcgag ccgatcgcct tcaaccggcg cgatctctgt
       61 cttgtctccg ggctggcgga actgatctcc catgaggtga tgaaggcgcg agtcgagaag
      121 gcgcggtcgc ggcatttcga ggtcctgatc acccaggtcc aggagtcgct ggcgatcttc
      181 tccgtggatg gccgcatgct ctacgagagc ccggcggcgc gggagatgct gggctatccc
      241 gatggcgtga tcgaaagcat gactcccgac gagctcctcg cgctgattca tcccgacgac
      301 ctcgccggga ccctcgaggt ccggcgcgag ttgctggcga atccaaacgc gtcacggagc
      361 tttctgcacc gcgtccggca tgtcgacggc tcctggcgga cgatacgagt cgccgccagc
      421 aatcttctgg acgagccgtc ggtcggtggc ctcgtcttta cctctcgcga cataacggag
      481 cagaatgccc acgacgagca actccagtac gaggcgcggc atgattcgtt gaccgggctt
      541 ctgaacaggg cgacgatcga gcgccacctt cacgggctgc tcgcggatcc tcgcgttccc
      601 gtggcttttg tctatatcga catcgatgat ttcaagctgg tcaacgatac cttcggccac
      661 ttcgccggcg atgaggtcct gcgcgtcgtg agtcgacgca tggacgcatt cgtccgcgaa
      721 ggcgaactcg tcgggagatt cggtggcgac gagttcgtga tgctgatccc ggacgtgagc
      781 tacgagtgcg cgcaagggcg cgccgaggaa ctcgcggacg tccttcagcg tccgatgcgc
      841 ctccagggca gtgatgtcgt tgcgatctgc agtatcgggg ttgcccgggc aaccgagcac
      901 ggcacggacc tcgacacgct ctttcgcgcg gcggatgcgg ccctctaccg ggtaaagagc
      961 tcgggcggtg gctctgtcgc gatgtacgcc acgtcgctgg acaatgccat ccacgaacgc
     1021 catgccatcg accacgagtt cacgctcgcg ctggagcgcg atgagctcgt tctagaatat
     1081 cagtcgatcg tcgaactcga gagcggggaa gtgatcggga tggaagctct cgtgcggtgg
     1141 aatcatccga cgcggggccg gctcatgccg atgagcttcg tgccgatcct cgaggagctg
     1201 ggaatcgccg gccgcctcgg ccggtgggtg ctcgatcgcg cggcacgtca ggtgaaacgg
     1261 ggcggcgtat cggtcaacgt caatgtctcg ccccagcatc tccgggagcc cacgttcatg
     1321 cgcgatgtcc tcgccgcgat cgagacgcat ggaatcgacg cgtcggcgct ggtcctcgaa
     1381 atcaccgaaa ccgcgtcgat cctccacgac agtgtgggaa tgacggtgct gagccggttg
     1441 aaggatcttg gcgtcacctt cgcgatcgac gacttcggca ccggatattc gggactctcg
     1501 gcgctcagga actatcccgt gcagatgatg aagctcgatc gcgtcttcct ggagcgcctc
     1561 gagtctgatc tccgggacca ggaaatcgtg cgcgcggtga tcaacgtggc caaggcgctc
     1621 gggatcgcgg tcgtggtcga ggggatcgaa tcgatgaccc aggcg
//
LOCUS       DASTMD010000358         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_925493, whole genome shotgun sequence.
ACCESSION   DASTMD010000358 DASTMD010000000
VERSION     DASTMD010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925493"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFQ54_05245"
     CDS             complement(<1..585)
                     /locus_tag="VFQ54_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEU0164430.1"
                     /translation="MAQVQNGGAFPNPVYAREILEVGFREGQRLLFPHMQAANEAHTL
                     MLAETGVLGRDHAATIFAALAQVVVDGVDSFSYEPQVEDLFFAVEKRIITLAGPAAGG
                     NLQIARSRNDLAAVMNRLFLREYSLTVQRAVTRLRAALIDLASEHVRTIMPGITHTQP
                     AQPTTLAHYLLGVLGPLERDSERLREGWARINRSP"
     gene            complement(867..1811)
                     /locus_tag="VFQ54_05250"
     CDS             complement(867..1811)
                     /locus_tag="VFQ54_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0164431.1"
                     /translation="MSTRDFEQPGDRETALGVADVVLGRDPLGARSYRGTIAGTGARD
                     VKRTIARYFLYAILLLGGILMIFPFIWMVISSLKMPQDIYTLSLIPPDPTLDNYRQVF
                     DKTEYVRWFVNSLVVATITTASVAFFDSLAGYTLAKFRFPGQSIIFILILSTLMVPTE
                     MLVIPWYMLSINLHWTDTYWGIMFPGVISAFGVFLMRQFFTGVPNDLIDAARLDGFSE
                     FRIFWKIALPLVKPAVAALCIFTFLGNWNAYIWPLIVTRSEQMRTLPVGIAFFSSEAQ
                     SSFNLIMAAASLATVPVLIIFVIFQRQIIKGIVLTGLK"
     gene            complement(1801..>2656)
                     /locus_tag="VFQ54_05255"
     CDS             complement(1801..>2656)
                     /locus_tag="VFQ54_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0164432.1"
                     /translation="AFIAIPLIFFLAIRIAPAGYAFWVSLHQWDPIALKRPFIGLDNY
                     RQIKDDEVFWKAMRNTWLYVIVGVPVSLVLSLVIALGLQRLVRYVGFYRVLYFVPYVT
                     SLVAVGWVWRWMYMPNGLFNDFLSRFAIGPFTFLGSPDTALYSILAMTIWQGLGFQII
                     IFLAGLESIPIVYQEAAAVDGATEWQRFRDVTFPLLNPTLVFLLVTGVINYLQVFTQV
                     RAMSAGGTGGPLNSTISLVLYVYQQAFTSLPSKMGYASAMTFVLFAMILAITILQLKV
                     LSRNNNEY"
BASE COUNT          589 a          786 c          837 g          444 t
ORIGIN      
        1 cggtgaccga ttgatccgcg cccaaccttc acgcaggcgt tcgctgtcgc gctcgagcgg
       61 tccgaggacg ccgagcaggt agtgggcgag cgtcgtcggc tgcgccggct gggtgtgcgt
      121 gatacccggc atgatcgttc gcacgtgttc ggacgcgagg tcgataagcg ccgcccgcaa
      181 ccgcgtgacg gcacgctgga cggtcagcga gtactcgcgc aggaacagtc gattcatcac
      241 ggcggcgaga tcgttgcgcg agcgggcgat ctggagatta ccgccggccg ccggcccagc
      301 caaggtgatg atccgcttct ccaccgcaaa aaagaggtct tcaacttgcg gctcatagga
      361 gaacgaatcc accccgtcga cgaccacctg tgcgagagcg gcgaagatcg tggcggcatg
      421 atcgcgaccg agaaccccgg tctccgcgag catcagtgtg tgtgcctcgt tggccgcttg
      481 catatgcggg aagagcaacc gctgaccctc gcggaacccg acttcgagaa tctcccgggc
      541 atagaccgga ttcgggaatg caccaccgtt ctgaacctgc gccattgctt ctcgctttca
      601 tattcgttca atccgcaacg gccagcggga cggagaggcg ggacacgaag atcccgccgt
      661 acgaacgacc cgcgtacgta cggcgtccgg gagtagggtg ggacttttat gtcccgccgt
      721 caggcgcagc ctgacatgat tcgtagcgaa tcgagcgtgc caaaaagccg gtgagactat
      781 tgcagactcg cttacggcgg gaccgccgta tcgggccgca atgcagcccg atcggcctgt
      841 ccggcctgcc ctgacctcct gcttcgtcac ttcaagccgg tcagcacaat ccccttgatg
      901 atctggcgct ggaagatcac gaagatgatc agcaccggca ccgtcgccag cgatgccgcc
      961 gccatgatca ggttgaacga actctgggcc tcgctcgaga agaaggcaat cccaaccgga
     1021 agcgtccgca tctgttccga ccgcgtgacg atcaagggcc agatgtaggc gttccagttc
     1081 ccgaggaagg tgaagataca gagtgccgcg acggcaggtt tcacgagcgg gagcgcgatc
     1141 ttccagaaga tccggaactc ggagaagcca tccagacgcg ccgcatcgat caggtcgttg
     1201 gggacaccgg tgaagaactg gcgcatcagg aagacgccga acgcgctgat caccccgggg
     1261 aacatgatcc cccagtaagt atccgtccag tgcaggttga tcgagagcat gtaccacggg
     1321 atgacgagca tctccgtcgg caccatcagc gtactcagga tcaagatgaa gatgatcgat
     1381 tgcccgggaa agcggaactt cgccagcgtg tagccggcga gcgaatcgaa gaatgccacg
     1441 ctggccgtcg tgatcgtcgc gacgacgaga ctgttgacga accagcggac gtattcggtc
     1501 ttgtcgaaga cctgccggta gttgtcgagt gtcgggtccg gcgggatcag cgagagggtg
     1561 tagatatcct ggggcatctt cagcgatgag atcaccatcc agatgaaggg aaagatcatc
     1621 aggatcccgc cgaggagcag gatcgcgtag agaaaatagc gcgcgatcgt ccgcttcaca
     1681 tcccgtgctc cggtgccggc gatcgtcccc cggtaactcc gcgcgccgag cggatcgcga
     1741 ccgagcacga catcggcaac gccgagcgcc gtttcccgat cacccggctg ctcgaaatct
     1801 ctagtactca ttgttgttcc tcgagaggac cttcaactga aggatggtga tcgcgaggat
     1861 catcgcgaag aggacgaagg tcatcgccga tgcgtacccc atcttcgacg ggagcgaggt
     1921 gaacgcctgc tgatagacat agaggacgag gctgatcgta ctgttgagtg gaccgccggt
     1981 gccgccggcg ctcatcgccc gcacttgcgt gaagacctgc aggtaattga tcacgccggt
     2041 cacgagcagg aagacgagcg tgggattcag caacgggaag gtgacatccc ggaagcgctg
     2101 ccactcggtc gcgccatcca ccgcggccgc ctcctggtag acgatcggga tgctctcgag
     2161 accggcgagg aagatgatga tctgaaaccc cagtccctgc cagatcgtca tcgcaagaat
     2221 cgaatagagc gcggtatcgg gactgccgag aaacgtgaac ggtccgatcg cgaagcggct
     2281 caggaaatcg ttgaagagac cgttcggcat gtacatccac cgccagaccc agccgacggc
     2341 gaccagcgat gtcacatagg gcacgaaata gagcacccga tagaatccga catagcggac
     2401 caggcgttgg agcccgaggg cgatgacgag gctgaggaca agactgactg gcacgccgac
     2461 gatgacgtag agccaggtat tccgcatcgc cttccagaag acctcgtcgt ccttgatctg
     2521 gcgatagttg tcgagcccga tgaacggacg cttcagggcg atcgggtccc actggtgaag
     2581 gctgacccaa aacgcatacc cggccggtgc gatccggatc gcaaggaaga agatcagtgg
     2641 aatcgcaatg aaggca
//
LOCUS       DASTMD010000359         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3068229, whole genome shotgun sequence.
ACCESSION   DASTMD010000359 DASTMD010000000
VERSION     DASTMD010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3068229"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFQ54_05260"
     CDS             <1..534
                     /locus_tag="VFQ54_05260"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164433.1"
                     /translation="VHEETLDCTVHFVITVQEEVGLRGARIVAYKLDPTAAIIVDTMP
                     SGGTPDVSSTRHLTMEIGKGPVLTLISQSGGGGAIAQPGMLRFIEGVAQQLEIGYQRA
                     LFYGGNSDAAAVHLVRSGIPTGIINLARRYSHSPVETLDLQDAVDTLHWLWACVQQFS
                     DETDLSFLGAVSLVDEA"
     gene            complement(493..1275)
                     /locus_tag="VFQ54_05265"
     CDS             complement(493..1275)
                     /locus_tag="VFQ54_05265"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEU0164434.1"
                     /translation="MLLTVDVGNTNTVFGVFELDGRRLLTTARASSRRDRMPDEWYAI
                     LAPVLLAEDIDPKGITAMVISSVVPNVTRWLTAMGEERLHVEPIVVGIDLDLGIGIDY
                     PTPREIGADRLVNSLAATHKYGFPIISIDFGTAINFDIVDINGNYVGGALAPGLVVAL
                     DAMVSRAARLFSVELVLPEKAIGRSTTDAIQSGVVLGYLGLLEGMIARIKAEMEGDPT
                     VIITGGYSEIFADASPMIDHHEPDLTIEGLRLVYERNRPQKA"
     gene            1372..>2102
                     /locus_tag="VFQ54_05270"
     CDS             1372..>2102
                     /locus_tag="VFQ54_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU0164435.1"
                     /translation="MSDPFPQPDPESPSTTKSPRRSRGSLPAIHWYTRMAAVFAMVLI
                     VGVAGGMLVERNIIAQPKPSTNEFDDLDAVAGILEDDYYYRPTDPTEAATWTTSLEQQ
                     AINGMLSSLDDQYTRYLNPPDARSASNQLAGTYQGVGISVGTSNDTVIVTAVTAGGPA
                     AKAGILKGDVVVSVDGRNVYPGDDVAGMIQGPAGSQVKIEIERGAATQSTTFSLTRVE
                     IVTPPVSYHIVPGTKIADIDIDIFG"
BASE COUNT          403 a          685 c          639 g          375 t
ORIGIN      
        1 gtgcatgagg agaccctcga ctgtaccgtg cacttcgtga tcacggtcca ggaggaggtg
       61 gggctccgcg gcgcccggat cgtcgcctac aagctcgacc cgacggccgc gatcatcgtc
      121 gacaccatgc cgtccggcgg cacgcccgac gtctcatcga cccgccacct gacgatggaa
      181 atcggcaagg gaccggtcct cacgctgatc agccagagcg gcggcggcgg tgcgatcgcg
      241 cagccgggga tgctgcgttt catcgagggt gtcgcccagc aactcgagat cggctaccag
      301 cgggcactct tctacggtgg gaactcggac gccgccgcgg tccatctcgt ccgctccggc
      361 atcccgaccg ggatcatcaa tctcgcacga cgatattccc attcacccgt cgagacgctc
      421 gatctccagg atgcggtcga tacgttgcac tggctgtggg cgtgcgtcca gcaattctct
      481 gacgagaccg acctaagctt tctgggggcg gtttcgctcg tagacgaggc gtagtccctc
      541 gatcgtgaga tccggctcgt gatgatcgat catcggtgag gcgtcggcga agatctcgct
      601 gtagccgccg gtgatgatca cggtcggatc tccctccatc tcggccttga tcctggcgat
      661 catgccttcc agcaatccga gatagccgag gacgacgcca gactggatcg cgtcggtcgt
      721 gctgcgaccg atcgccttct cgggcaacac caactccacc gagaaaaggc gggcggcacg
      781 actgaccatc gcatccaggg cgaccacgag tcccggcgcg agtgcaccgc cgacatagtt
      841 gccgttgata tccacgatat cgaaattgat cgccgtgccg aagtcgatcg agatgatcgg
      901 gaagccatat ttgtgcgttg ccgcgagaga gttcacgagg cgatcggcgc cgatctcgcg
      961 tggagtcggg tagtcgatcc cgatcccgag atccagatcg atcccgacga cgatcggttc
     1021 gacatggagc cgctcctctc ccatcgcggt cagccagcgc gtcacgttgg ggacgacgct
     1081 gctgatcacc atcgcggtga tccccttcgg gtcgatgtcc tccgcgagca agacaggcgc
     1141 gaggatggcg taccattcgt cgggcatccg gtcccggcgg gatgaagccc gcgcggtcgt
     1201 caacagccga cgcccgtcga gctcgaagac accgaagacg gtgttggtgt ttccgacatc
     1261 aaccgtcagt agcatgggtt ctccctatcc caacaatcct gcaatcctga aactttccgg
     1321 tccgcatcca gccaacatcg atgaccgcgc cgaacgtcga atggagcacg agtgtccgac
     1381 ccgtttcccc agcccgatcc cgaatcgcca tcgacgacca agagcccccg gcgatcacgc
     1441 ggctcgctgc cggcaatcca ctggtacacg cgcatggccg cggttttcgc gatggtgctc
     1501 atcgtcggcg ttgccggtgg catgctcgtc gagcgcaata tcattgcaca accgaagccg
     1561 tcgacaaatg aattcgacga tctcgacgcc gtcgcgggca tcctcgagga cgactactac
     1621 taccgtccca ccgatcctac tgaagcggcg acgtggacga cgtcgctcga acaacaggcg
     1681 atcaacggta tgttgagcag cctcgacgat caatacaccc ggtatctgaa tccgccggac
     1741 gcccggagtg cctcgaacca gctcgccggt acctatcagg gtgtgggcat atcggtcggg
     1801 acgagcaatg acacggtcat cgtcacggca gtgacggccg gcggaccggc ggcgaaggcg
     1861 gggatcctga agggcgacgt tgtggtctcg gtcgacggcc gaaacgtcta tcccggtgat
     1921 gacgtggccg ggatgatcca gggaccggcc ggctcgcagg tcaagatcga gatcgagcgc
     1981 ggcgccgcga cccagtcgac gaccttttcc ctgaccaggg tcgagatcgt gactccgccc
     2041 gtctcctatc acatcgttcc tggcacgaag atcgccgaca tcgacatcga catcttcggc
     2101 ga
//
LOCUS       DASTMD010000360         5343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1373174, whole genome shotgun sequence.
ACCESSION   DASTMD010000360 DASTMD010000000
VERSION     DASTMD010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373174"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VFQ54_05275"
     CDS             complement(<1..1015)
                     /locus_tag="VFQ54_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164436.1"
                     /translation="MQVTRDAEFATREPRWQVWRPDILALLVLALMTVVVCWHRFHYD
                     NWLTRHDLLAYFEPWYGYLGNRLSHFQIPAWNPYIYSGTPFAGDPESGWWYFPAMLAF
                     PFFTVATGFKVYILIQLVIAGSTTYALGRVLGFGVWGAMLAATVYEFGPFLYGQTDCC
                     TVGTQLETWVPLALLGVELAYRSRRVYALLSWWFVTGLAISQVILGWVGQGSFDGMLV
                     IAAWIGYRAIISPVEDWSFLRRVVKAVVTGGVVVVAGLAIGAAGILPKLAFDSESTNP
                     GGSYTGLVGAHDGAYLTFFQSIQAIFYTAYSSGSTAWGGTAIVLAILAIAFCGRNYAV
                     PFFL"
     gene            complement(1207..3675)
                     /locus_tag="VFQ54_05280"
     CDS             complement(1207..3675)
                     /locus_tag="VFQ54_05280"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU0164437.1"
                     /translation="MQATEDAVFAPGERRWQVWRPYILALVALGIMTAIVCWHRFLFD
                     NWLSRHDLLSFFIPWYGYLGDRLSHFDIPAWNPYLFSGSPFAADPESGWWYLPAMLAF
                     PFFGVATAFKMYILIQLVIAGSTSYALGRVLGFGVWGAMLAATIYEFGPFLYGQTDCC
                     TVGTQVETWIPLALLGVELAFRSKHLYSLISWWFVAGLAISQMILGWVGQGAFDSMLI
                     VAAWVAYRAIISPVNDWKFLRRVVEAVITGGAIVVCGLAIGAAGILPKLAFNSDSTNP
                     GGTYEGLTGAYDSPFLTLYQVTKTIFYATYAYGGSAWGGTAIILAFLAIAVCGRKYSV
                     PFFLVVTIVIYMLAMGTQPVLFLFDLIPGFKNIHIHSPGRVTWLQMIGPSMLAGAALK
                     GLLDKRGRYLYAPLALIPLGLVLIVAHWFSTRPLWFGWPMYISTIVVTVLAIVILCIP
                     KDGGFTRWSLTVPWEAVARTAAAIMIALAFFFPAGLNVLNHFVEVDDNPTEQLMWNTD
                     PVTQAAIARNISTSDPGTAAQFLQDQQATQQPFRYVGYGGNHVDGAFSLPDHRLFPGT
                     MAILVNGRPVRLQLQQIQGYNPLQLKDFSAFMAALNGGKQNYHFQDLWQNGTQSPLLD
                     MLNVRYIVVSKLTPSDRADVQALAQGRKIVYSDADVYVYANPDAYNRAWIVHDVRQAT
                     DTDGLAQLASGKVDGHQVAFINGKLPTVSAASGASSETSTVTSYAPEKIVISAHANSS
                     GLLVLSEIYDKNWKATVNGKSVDVYKTNGALRGVAIPAGDSEVVMTYEPTAQRIGLFV
                     SGGAGIVMLATFATAGWYTLRRRG"
     gene            4016..>5343
                     /locus_tag="VFQ54_05285"
     CDS             4016..>5343
                     /locus_tag="VFQ54_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164438.1"
                     /translation="MTVLTDAYSALRHHRYAKRFPDVIALAVVALIAAMVAVRLFNGQ
                     TIVLGRETIVRDLPMWTYLGERLHSLELPAWNPFQFGGIPFAADPASGWMYLPVVILF
                     ALFSVTKATAALLVAHFAIAGIGIFIFARVIGIERPAAATAGIAFISSNWFYTASFSD
                     PTQVAVASWLPWALLFTELAIRPETLFRRIAFTGLAAASFSQILGIWYGNGAFYAVIL
                     LVAWLAYRTLVETWGDEIGLQRHLIDLAIVLAGVIVWTVGLGAAGLFPRALAEKTSQF
                     SLPIVDFSGWNLGKALTGDGAASPGDVVIYLAIIGAFVAGRKFCNPFFIGAVILAFLV
                     ETDQFMVVGSLPIAILAGIGVTMLITKPVPDRQGVIAIGAMVVVFLATLFSANHMTET
                     ERMVEFALVVALSALILARDMSSWRYLLIAFALLFHATMLLDRVDLLKYG"
BASE COUNT         1005 a         1726 c         1666 g          946 t
ORIGIN      
        1 cgagaaagaa cggcaccgcg tagtttcgcc cgcagaatgc gatggccagg atcgcgagca
       61 cgatcgccgt gccgccccag gccgtcgagc cggacgagta ggcggtatag aagatcgcct
      121 ggatcgattg gaagaacgtc agatacgccc cgtcatgcgc gccaacgagc cccgtgtaac
      181 tgccaccggg attggtcgac tcggagtcga acgcaagctt cggcaggatc ccggcagcac
      241 cgatcgcaag accggcaacg accacgacac cgccggtgac cactgccttg acgactcggc
      301 ggaggaaact ccaatcctcc acgggggaga tgatcgcgcg gtacccgatc cacgccgcga
      361 tgacgagcat cccgtcgaac gagccctgcc cgacccatcc gagaattacc tgactgattg
      421 ccaggccggt cacgaaccac caggagagca gcgcgtagac gcgtctcgat cggtacgcca
      481 gctcgacccc gagcagggcg agcggaaccc acgtctccag ctgggttccg acggtacagc
      541 agtcggtctg cccgtagagg aacggcccga actcgtaaac ggtggccgcg agcatcgcgc
      601 cccacacgcc aaacccgagc acccgtccca gcgcgtaggt cgtcgacccc gcgatcacga
      661 gctggatcag gatgtagacc ttgaagccgg tcgccacggt gaagaaggga aacgcgagca
      721 tggcggggaa gtaccaccag ccggactcgg ggtccccggc gaaaggggtc ccgctgtaga
      781 tgtatgggtt ccacgccggg atctgaaaat gcgagaggcg attccccagg tagccgtacc
      841 agggttcgaa gtaggcgaga aggtcgtgcc gcgtgagcca gttgtcgtag tgaaagcgat
      901 gccagcagac gactaccgtc atcagtgcga gcaccaggag ggcgaggatg tcggggcgcc
      961 agacttgcca gcggggttcg cgcgtggcga actctgcatc ccgcgttacc tgcatgaacg
     1021 gccctcgctc ttctcggtca gttcggatgt cgctcgtcgc gccggacacg gccgacctcg
     1081 catacggggg tcaaactcgc cgtaccgtac cacgccatcc tctgtccctg aaacaagcgg
     1141 ccgcgaccga ttactccccc cctctgcggg acttccggcg cgtatattcc gcgatggtcc
     1201 tacgactcat ccccgacggc gcagcgtgta ccaccccgcc gtcgcgaagg tcgccagcat
     1261 cacgataccc gcgcccccac tcacgaagag cccgatccgt tgtgcggtcg gctcgtaggt
     1321 catcacgacc tcgctgtcac cagccgggat cgcgaccccc cggagcgcgc cattcgtctt
     1381 gtagacgtcg acgctcttcc cgttcacggt cgccttccag ttcttgtcgt agatctcgct
     1441 gaggacaaga agacccgacg aattcgcgtg agccgagatc acgatcttct ccggcgcata
     1501 ggaggttacg gtcgaggtct cggaactggc cccgctcgcc gccgacacgg tcggcagctt
     1561 gccgttgatg aacgcgacct ggtgaccatc caccttcccc gacgccagct gcgccagacc
     1621 gtccgtgtcc gtcgcctggc ggacatcgtg cacgatccac gcccggttat aggcatccgg
     1681 attcgcatag acatagacgt cggcatccga atagacgatc ttgcggccct gggcgagtgc
     1741 ctgcacgtcg gcgcgatccg agggggtgag cttcgagacc acgatgtagc ggacattcag
     1801 catgtcgagc aggggagact gcgttccgtt ctgccagagg tcctggaagt ggtaattctg
     1861 tttgccgccg ttcaacgccg ccatgaaggc agagaagtcc ttgagctgga gcgggttgta
     1921 tccctggatc tgctggagct ggagtcgaac tggtcgcccg ttcaccagga tcgccatcgt
     1981 cccggggaag agtcgatggt caggcaggga gaaggcgccg tcgacgtgat tgccgccgta
     2041 gccgacgtag cgaaagggct gctgggtcgc ttgctggtcc tggaggaact gcgccgccgt
     2101 cccgggatcc gacgtggaga tattccgggc gatcgcggcc tgcgtcaccg gatccgtatt
     2161 ccacatcagc tgctcggtcg ggttgtcatc gacctcgacg aagtggttga ggacgttcag
     2221 acccgccggg aagaagaatg cgagggcgat catgatcgcc gccgccgtcc gtgcgactgc
     2281 ctcccagggc acggtgagcg accatcgcgt aaagccgccg tccttgggga tgcagagaat
     2341 gacgatcgcg agaaccgtca cgacgatcgt cgagatgtac atcggccagc cgaaccagag
     2401 cggccgggtc gagaaccagt gggcgacgat caggacgaga ccgagcggga tcagggccag
     2461 tggtgcatag agatagcgac cgcgtttatc gagcagcccc ttcagcgcgg cgcctgccag
     2521 catcgagggg ccgatcatct gcaaccaggt gacccgtccc ggactgtgga tgtggatatt
     2581 cttgaatccc gggatcagat cgaacaggaa gaggaccggc tgcgtgccca tcgccagcat
     2641 gtagatgacg atcgtgacca ccaggaagaa gggaacggag tacttgcggc cgcagaccgc
     2701 gatcgccagg aacgcgagga tgatcgccgt cccaccccag gccgagccgc cataggcgta
     2761 ggtggcatag aagatcgtct tcgtcacctg atagagggtc agaaagggcg agtcgtacgc
     2821 cccggtgagt ccctcatacg tcccgccggg attcgtcgag tcagaattga acgcgagttt
     2881 cggcaggatg ccggccgcgc cgatcgccag gccgcaaacg acgatcgcac cacccgtgat
     2941 caccgcctcg acaacccggc gcaggaattt ccagtcattc acgggcgaga tgatcgcgcg
     3001 atacgccacc cacgccgcga cgatcagcat cgagtcgaac gcaccctgcc caacccagcc
     3061 gaggatcatc tggctgatcg ccaggccggc gacgaaccac caggagatca acgaatagag
     3121 gtgtttggac cggaacgcga gttcgacacc aagcagcgcc aaaggaatcc aggtctcgac
     3181 ctgtgtcccc accgtacagc agtcggtctg tccgtatagg aatggcccga actcgtagat
     3241 cgtggcggcg agcattgccc cccacacgcc aaacccgagc acccgaccca gcgcgtagct
     3301 ggtcgacccc gcgatcacga gctggatcag gatgtacatc ttgaaggcgg tggcgacccc
     3361 gaagaacggg aacgccagca tcgccgggag ataccaccag cccgattcgg gatccgccgc
     3421 gaacggcgag ccactgaaga gataggggtt ccacgccggg atatcgaaat gggaaagccg
     3481 atcgccgagg tagccatacc aggggatgaa gaagctgagg aggtcgtgcc ggctcaacca
     3541 gttgtcgaag aggaagcgat gccagcagac gatcgcggtc atgatcccca gtgcgaccag
     3601 tgcgaggatg tagggacgcc acacctgcca ccgtcgctcg cctggggcga acaccgcatc
     3661 ctccgttgct tgcatgaact gccctcgctc ttccctcaag tagtgcgctt cgtgccggac
     3721 ggcggcggcg cccgctggac ctgtcccact ctaacgtacc atgcctcacg gatttcacga
     3781 aacagccgca tgcggcgcgt accagtgacc ccccggcggt ccgttgtgcc ggtatcggga
     3841 aggttgcggt ttgcgaagga tgcggccata cctgcccagc tttgcccgtg attccgcaag
     3901 ttgtccgctg cagccacccc ggtggacgtg ccccaacgcg cgagttctgc cgaaatctgg
     3961 cacacttacg gtcgggttgg ggtggggtgg tgattcgcta aaacgtgggg ggaagatgac
     4021 cgttcttaca gacgcgtatt cggcactgcg acaccatcgc tatgcgaagc ggtttccaga
     4081 tgtcatcgcg cttgcggtcg tggcgttgat cgccgcaatg gtcgcggtga ggctcttcaa
     4141 cggtcaaacg atcgtccttg gccgggaaac gatcgttcgc gatcttccga tgtggacgta
     4201 ccttggcgag cgactgcact cgctcgagct cccggcctgg aatccgttcc agttcggtgg
     4261 catccccttc gcggccgatc ccgcgtccgg ctggatgtac ctcccggtcg tgattctctt
     4321 cgcgctcttt tcggtcacga aagcgacagc ggctcttctc gtcgcgcatt tcgcgatcgc
     4381 cggaatcggg atcttcatct tcgcgcgggt gatcggcatc gagcgccctg ccgccgcgac
     4441 ggccggtatt gccttcatct ccagcaactg gttctacacc gcgtcgttca gtgacccgac
     4501 ccaggtcgcc gtcgcgtcat ggctcccgtg ggccctcctc ttcaccgaac tcgcgattcg
     4561 accggagacc ctcttccgcc gaatcgcctt caccggactt gccgccgcgt ccttcagcca
     4621 gatcctcggc atctggtacg gcaacggcgc gttctacgcc gtcatcctcc tggtggcctg
     4681 gctggcctat cgcacgctcg tcgagacctg gggtgacgag atcgggctcc agcgacatct
     4741 catcgacctt gcgatcgtgc tcgccggggt gattgtctgg acggtgggcc ttggcgcggc
     4801 cgggctcttc ccgcgtgccc tggccgagaa gacctcgcag ttctcgcttc ccatcgtcga
     4861 tttctcgggc tggaacctcg gcaaggcact caccggcgat ggcgccgcga gcccgggcga
     4921 tgttgtcatc tatctcgcga tcatcggggc gttcgtcgcc ggccggaagt tctgcaaccc
     4981 gttcttcatc ggtgcggtta tcctcgcgtt cctggtcgaa acggaccagt tcatggtcgt
     5041 gggaagcctg ccgatcgcga tcctcgccgg aatcggcgtc acgatgctca tcacgaagcc
     5101 ggttcccgat cgccagggcg tgatcgccat cggcgcgatg gtcgtcgtct tcctcgcgac
     5161 gctgttcagc gccaaccaca tgaccgagac cgagcgcatg gtggagttcg ctctggtcgt
     5221 ggcactctcc gcgttgatcc tcgcgcgtga catgtcatcg tggcggtatc tgctgatcgc
     5281 gttcgcgctg ctcttccacg ccacgatgct tctcgatcgg gtcgatctgc tgaagtacgg
     5341 cga
//
LOCUS       DASTMD010000361         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2890879, whole genome shotgun sequence.
ACCESSION   DASTMD010000361 DASTMD010000000
VERSION     DASTMD010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2890879"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..733)
                     /gene="scpB"
                     /locus_tag="VFQ54_05290"
     CDS             complement(32..733)
                     /gene="scpB"
                     /locus_tag="VFQ54_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU0164439.1"
                     /translation="MTETIANPTPDQIDDLPGTQLPIPGTESLHSITIPELAALMEAF
                     LLVATEQPTIDDLAKGAGVEPDQIELALARINEDEGRGWVVQKHGRKVQLATAPRFAD
                     RVRTFLGLDRESRLSSAAIETLAIVAYQQPVTRAEIDAVRGFDSSGVLATLHSRGLIE
                     PVSRLQTVGNPIQYGTTAEFLQHFGLRSLSELPPLGQIEGRDARDALDAAAQAGRPDD
                     MEPLASSDALTGEDA"
     gene            complement(749..1540)
                     /locus_tag="VFQ54_05295"
     CDS             complement(749..1540)
                     /locus_tag="VFQ54_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU0164440.1"
                     /translation="MDETIEIAPLTLANYQLRLPAFEGPLDLLLRLIERSQLAITDVS
                     LTTVTSQFLEIIQEMRETTPAETIAEFAAVGTRLTLLKSRSLLPRPPRSEDEEAPQSD
                     LTIELAEYKRLKEIAIQLGERFASGYSLFAPATRLPVSIPTEPKDVRLVPYEANVLLR
                     SLRRRLTTVPRPIQMIRQRKIVSIREMIDRVIHLTSFRKAISFTTLTAEYQTRTEVAT
                     AFLAVLVLVRRRSLLAAQGDLFSEITLEPQNAEEAAADDLDEFIN"
     gene            complement(1524..>2104)
                     /locus_tag="VFQ54_05300"
     CDS             complement(1524..>2104)
                     /locus_tag="VFQ54_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU0164441.1"
                     /translation="IPERAARVRYRIAQALAFAGRSGAPDIPARIRELADDATIAQLR
                     RLSAQDQRHLIAVCDALQRQGASDELALAGLLHDIGKEHEGRRPSVVDRSAKVVLSRI
                     WPSGLARFLARSQALRFGGGLWIAGNHARLGATIAEELGVSDRVVWLIAHHEDRDPTD
                     ANLRRLQQADDDGRSIPAASFREGGPRHGRDD"
BASE COUNT          354 a          666 c          703 g          381 t
ORIGIN      
        1 acgacgcctc cctggagaag aacgtcgtgg ctcacgcgtc ttctcccgtc agcgcgtcgg
       61 acgatgcgag cggttccatg tcgtccggcc gtcctgcctg cgcggccgcg tcgagagcgt
      121 cgcgcgcgtc tcttccctcg atctggccca ggggagggag ttcggacagc gagcgcagac
      181 cgaagtgctg gaggaattct gccgtcgttc cgtactggat cggattcccg accgtctgca
      241 ggcgcgagac cggctcgatc agaccgcgcg aatgcagggt cgcgagcacg cccgaactgt
      301 cgaagccgcg gacggcatcg atctcggcac gggtgaccgg ctgctggtag gcaacgatcg
      361 ccagggtttc gatcgcggcc gaggagagtc gcgattcgcg atcgagaccg aggaaggtcc
      421 ggacgcgatc ggcgaatcgc ggcgccgtcg cgagttggac tttccgcccg tgcttctgga
      481 cgacccatcc acgtccttca tcctcgttga tccgggccag ggcgagttcg atctgatccg
      541 gttcgacgcc cgctcctttc gcgagatcgt cgatcgtcgg ttgctcggtg gcgacgagga
      601 ggaaggcctc catcagtgcc gcgagttccg gaatcgtgat cgagtgaagc gactcggtgc
      661 ccggtatcgg aagctgggtt cctggcaggt catcgatctg atcgggcgtg ggattggcaa
      721 tggtctctgt catcgcgtgc tcgtggtcct agttgatgaa ctcgtcaaga tcgtccgccg
      781 ccgcttcctc cgcgttctgg ggctcgagcg tgatctcgga aaagaggtcg ccctgcgccg
      841 caagaagaga ccttcggcgg acgagtacca ggacggcgag aaacgccgtg gcaacctcgg
      901 tccgcgtctg gtactcggcg gtcagtgtgg tgaaggagat cgccttccga aacgacgtca
      961 ggtggatcac gcgatcgatc atctcgcgga tgctgacgat cttccgctgg cggatcatct
     1021 ggatcggtcg tggcaccgtc gtcaggcgtc gccgcaggga ccgcaggagg acattcgcct
     1081 cgtacgggac gagcctgaca tccttcggct cggtcgggat cgaaaccgga agccgcgtcg
     1141 ccggggcgaa gagcgaatat ccagaggcga agcgctcgcc gagctggatg gcgatctcct
     1201 tcagccgctt gtattccgcg agctcgatcg tgagatcgct ctgcggcgcc tcctcatcct
     1261 cggaacgcgg aggacgaggg agcagcgaac gactcttgag cagggtgagc cgcgtcccga
     1321 ccgcggcaaa ctccgcgatc gtttccgcgg gggtcgtctc ccgcatttcc tggatgatct
     1381 cgaggaactg gctggtgacg gtcgtcagcg agacgtccgt gatcgcgagc tggctccgct
     1441 cgatgaggcg gagaagcagg tcgagcggcc cctcgaaggc cggcagtcga agttgatagt
     1501 tggcgagcgt cagcggcgcg atctcaatcg tctcgtccat gcctcggtcc tccttcccgg
     1561 aaactcgctg ccggaatcga cctgccatcg tcatcggctt gctgcaatcg gcggagattc
     1621 gcatcggttg gatcgcgatc ctcgtgatgc gcgatcagcc atacgacgcg atccgaaacg
     1681 ccgagttcct ctgcgatcgt ggcaccgagc cgcgcgtgat tcccggcaat ccagaggcca
     1741 ccaccgaaac ggagcgcctg tgaccgggcg agaaacctcg ccaatccgga gggccagatg
     1801 cgagacaaca cgaccttcgc gctccgatcg accacgctcg ggcgacgacc ttcgtgctcc
     1861 ttgccgatgt cgtggagcag gccggcgagt gccagttcgt cggacgcccc ctgccgttgg
     1921 agcgcatcgc agacggcgat cagatgccgc tggtcctgcg ccgaaagtct gcgcaattgc
     1981 gcgatcgttg cgtcatcggc cagctcccgg atgcgcgccg gaatgtcggg agcgccggat
     2041 cggccggcga acgccagcgc ctgcgcgatc ctatacctga cccgcgccgc tcgttccgga
     2101 atca
//
LOCUS       DASTMD010000362         4000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1730724, whole genome shotgun sequence.
ACCESSION   DASTMD010000362 DASTMD010000000
VERSION     DASTMD010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730724"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFQ54_05305"
     CDS             complement(<1..480)
                     /locus_tag="VFQ54_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU0164442.1"
                     /translation="MSTGGEAYQHSHDHEADGDRTPLRPLGIALAITAGFMIVEAVGG
                     YLTNSLALLADAGHMATDVIALCLSLFAVWIAKRPATPSRSFGYLRSEVLAALANSTA
                     LIVISLIVFWEAFRRIGDPPHVDSGPMIIVAFAGLIANLASARVLSRGGGHQHNLNTR
                     "
     gene            1390..1776
                     /locus_tag="VFQ54_05310"
     CDS             1390..1776
                     /locus_tag="VFQ54_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0164443.1"
                     /translation="MRILLVEDEPHVRIALSRTLIAWGYQVTEAATGRDALATLAAAA
                     FDLLILDVNLPDATGWDVMRGLSTTPSAQSPVIVISAVPPSSARIREFRPFGVLMKPF
                     PIEALHRLVRNAEAGEPAHAVGTESD"
     gene            1916..2245
                     /locus_tag="VFQ54_05315"
     CDS             1916..2245
                     /locus_tag="VFQ54_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164444.1"
                     /translation="MASERERQWDRNVVAAAHTVWANDDVVAAYVVLPSRPSYRQLSH
                     YRDYEKDLNIRLTVLARGGVFVQESEPVEALTPLSASSLPERDSPRGHHAPVWLHDFL
                     LVTEGVR"
     gene            2242..2331
                     /gene="kdpF"
                     /locus_tag="VFQ54_05320"
     CDS             2242..2331
                     /gene="kdpF"
                     /locus_tag="VFQ54_05320"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEU0164445.1"
                     /translation="MSSGDWIVLIIGIVLLGYLVYALLRPERF"
     gene            2366..>4000
                     /gene="kdpA"
                     /locus_tag="VFQ54_05325"
     CDS             2366..>4000
                     /gene="kdpA"
                     /locus_tag="VFQ54_05325"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEU0164446.1"
                     /translation="MTFIGIVQILVYLVILAAITKPLGIYMTRLFEGERVFLTPVLRP
                     VERGIYRISGVDERREMRWTTYAVAMLLLQVVGFIAVYLFQRFQHGLPLNPQKMVDIE
                     PRSAFNTAISFVTNTNWQGYVGETTMSYLTQMSALTVQNFLSAATGIAIAIALIRGFS
                     RRSASQIGNFWVDVTRAVLYILLPISIVMAIFFMARGVPQNFKDYVDATGIGGFTQTI
                     AMGPVASQEAIKMLGTNGGGFFNANSAHPFENPTPLTNFIQMLSILSIPAGLTYVFGK
                     MVGNTKQGWAIFATMASLLVVGIIVTTAAEQHGNDLFRALDVSQGTSLLGHSMPGGNM
                     EGKELRFGINTSSLFAVITTAASCGAVNAMHDSFTPLGGLIPMANIAVGEVIFGGVGS
                     GLYGMLVFAVVAVFVAGLMGGRTPEYIGKKIEAFDVKMAMLTLLILPLSILGFVAISA
                     VTGYGLNTVFNPGPHGLSEILYAFTSATGNNGSAFAGIGANTPWYNTALGIAMGAGRF
                     MMIVPVLALAGSLAAKKRHASNAGTFPTTGGLWVGLL"
BASE COUNT          693 a         1234 c         1292 g          781 t
ORIGIN      
        1 ccgcgtattg aggttgtgct ggtgcccacc gccccgggag agcacccggg ccgaggcgag
       61 attcgcgatc aggccggcga aggcaacgat gatcatcggc ccactgtcga cgtgcggggg
      121 atcgccgatg cgccggaacg cctcccagaa gacgatcagg gagatcacga tcagcgccgt
      181 cgaattcgcc agcgcggcga ggacttcgga gcggaggtag ccgaacgaac ggctcggcgt
      241 cgccggccgt ttcgcgatcc agacggcaaa gagggaaaga cagagggcga tgacgtcggt
      301 ggccatgtgg ccggcatcgg cgaggagggc gagcgagttg gtgaggtagc caccaaccgc
      361 ctcgacgatc atgaagccgg cggtgatcgc cagggcaatg ccgagcgggc ggagcggcgt
      421 tcgatcgccg tcggcttcat gatcgtggct gtgttgatac gcttcaccac ctgtcgacat
      481 cacattgctc cctggtccgc gctcgccaga cattctcccg attgaaacct tgtctcatcg
      541 tgtgcatcgc catgcacacg ttctgctaag tggaattcta gcatgcgacg tgtgcatgaa
      601 caagcacgcg ttttcttcgt cacgtatccg ccgtcaacgt catcccgagc ttgtcgaggg
      661 accgcgtggc tgctgtgtgc ttcccgtaca acgcctctcc gcagggtggg gcggtgaggc
      721 aacgcgaatg tggtccctcg acaagctcca ccccacgcct gacggcgtcg cagctgcgct
      781 ctgctctggt gacataacgt cgaggtgagt cgcgcagccc gggatcgtgt cgggatcacg
      841 tgcttctacc cgaccccgtc ccactcgtca ccctgagccg agcgaaggac ccctgcgcga
      901 agcgcagtcg gccagcggcc gacatgatgt tggctggaac cctgctctcc ccaatgtgtt
      961 catccgtaac gccgtagaca cggcttttat ggccgccccg gctcggcgga gccgatccgt
     1021 ttccgaggag caacgcgcag gatgaggtgc cgttggagcc cgccgttggc gggtgcgctt
     1081 cgtgcgggga tttctcgaca ggctcgaaat gacgagtggg taggcgaccg cgttggggcc
     1141 tcaagggcgc tgtgcgtcgg gaggtcatat cgagctgcgc tcgatgggtc acacgggccc
     1201 gccacgggtg tggagggggg tgacccgctg cgggtgtgga cggggtgtga cggcggattc
     1261 tgatacttct ctaatctccc ccggcgcctt cctaacgcgg ccctaacggc gccgaatcta
     1321 cccttgctca tgtgcgaatt gaagatccgc aggccgtctg cggcagttcg gcaaggagac
     1381 gatcgcgcaa tgaggattct gctggtagag gacgaaccac acgtacggat cgcgctcagt
     1441 cgcacgctca tcgcctgggg ataccaggtg acggaagccg cgaccggtcg cgatgcgctc
     1501 gcgacgctcg ccgccgccgc cttcgatctt ctcatcctcg atgtcaacct gccggacgcg
     1561 accggctggg atgtgatgcg cggactgtcc acgactccat ctgcccagtc gccggtgatc
     1621 gtcatttccg ccgtccctcc cagttccgcc cgcatccggg aattccgccc cttcggcgtg
     1681 ctgatgaagc cgtttccgat cgaggcgttg caccgtctgg tgcgcaatgc ggaagcgggg
     1741 gagccggcgc atgccgtcgg gaccgagtcg gattgatgcg gtctgctcag gaaaggagtc
     1801 tcaccgatgt gggacatcgt gatggtcgga cttggcgtga tcaccttcat cgtgctcatc
     1861 gcctacgtct atgcgtgcga gaggttgtag tcgtgaaaga ccaggaggcg tcgtgatggc
     1921 gagcgagcga gagcgtcagt gggatcgaaa tgtggtggcc gcggcgcata cggtctgggc
     1981 gaatgacgat gtcgtggctg cgtacgtcgt gctgccgagc aggccgagct atcgccagct
     2041 ctcccactac cgggactacg agaaggatct gaacatccgg ctcacggtgc tggcgcgggg
     2101 tggcgtcttc gtccaggaga gcgagccggt cgaagcgctc acaccgctct ccgcgagttc
     2161 gctcccggaa cgggactcgc cacgcggaca tcacgcgccg gtctggttgc acgactttct
     2221 gttggtgacg gagggagtga gatgagcagc ggcgactgga tcgtcctcat catcgggatt
     2281 gtgctgttgg gatatctggt ttacgcgctc ctgcgaccgg aacggttcta gggcagtgcg
     2341 gtagggaaat gcgagggaga acccggtgac attcatcggt atcgtgcaga tcctcgtcta
     2401 tctggtgatc ctggcggcga ttacaaagcc gctcggcatc tatatgacga gacttttcga
     2461 aggggagagg gtcttcctca ccccggttct gcgcccggtc gagcgcggaa tctatcgcat
     2521 cagcggggtc gacgagcgcc gggagatgcg ttggacgacc tatgcggtcg cgatgctgct
     2581 gctccaggtc gtcggcttca tcgccgtcta cctcttccag cgcttccagc acggcttgcc
     2641 gctcaacccg cagaagatgg tggacatcga accgcgttct gccttcaata ccgccatttc
     2701 gttcgtgacc aacacaaact ggcaaggcta cgtcggcgag acgacgatga gttacctgac
     2761 ccagatgtcg gcactcaccg tccagaactt cctctcggcg gcgaccggca ttgcgatcgc
     2821 gatcgccctg atccggggat tctcccgccg ctccgcgagc cagatcggca acttctgggt
     2881 tgatgtcaca cgggcggttc tctacatcct cctcccgatc tcgatcgtga tggcgatctt
     2941 cttcatggcc cgtggcgttc cccagaactt caaggactac gtggatgcga ccggaatcgg
     3001 tggcttcacg cagacgatcg cgatggggcc ggtcgcttcg caggaagcga tcaagatgct
     3061 tggcacgaac ggcggtggct tcttcaatgc caactccgcg catccgttcg agaatccgac
     3121 accgctgacc aacttcatcc agatgctctc gattctctcg atcccggccg gattgacgta
     3181 tgtcttcggc aagatggtgg gcaacacgaa gcagggctgg gcgatcttcg caacgatggc
     3241 ctcgctcctc gtggtcggca tcatcgtgac gacggccgcg gagcagcacg gcaacgatct
     3301 cttccgcgcg ctcgatgtca gccagggcac cagcctgctt ggccactcga tgccgggcgg
     3361 caacatggag ggcaaggagc ttcgattcgg aatcaacacc tcctcgctct ttgcagtcat
     3421 caccaccgcg gcgtcctgcg gtgcggtcaa cgcgatgcac gattcgttca ccccgctcgg
     3481 tggcctgatc ccgatggcga atatcgccgt tggtgaggtg atcttcggcg gtgtcggatc
     3541 cggtctctac ggcatgctcg tcttcgcggt agtcgcggtc ttcgtggccg gactgatggg
     3601 cgggcgcacg ccggaataca tcggcaagaa gatcgaggca ttcgacgtca agatggcgat
     3661 gctgacgctg ctcatcctgc cgctctcgat cttgggcttc gtcgcgatct cggcagtgac
     3721 gggatacggt ctgaacaccg tcttcaaccc cggtccacac ggactctcgg agatcctcta
     3781 cgcctttacc tcggcgacgg gcaacaacgg gtcggcgttt gccggaatcg gtgccaacac
     3841 gccctggtac aacaccgccc tcgggatcgc gatgggagca gggcggttca tgatgatcgt
     3901 cccggttctc gccctcgccg gttcgctggc ggcgaagaag cgacacgcca gcaacgccgg
     3961 gaccttcccg acgacgggcg gcctctgggt cggtctgctc
//
LOCUS       DASTMD010000363         1880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_122309, whole genome shotgun sequence.
ACCESSION   DASTMD010000363 DASTMD010000000
VERSION     DASTMD010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122309"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..902
                     /locus_tag="VFQ54_05330"
     CDS             204..902
                     /locus_tag="VFQ54_05330"
                     /inference="COORDINATES: protein motif:HMM:NF022247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2604 domain-containing protein"
                     /protein_id="HEU0164447.1"
                     /translation="MIEVEQKWVFEVDGVSIATDDSKLKGRDVRTLAGLVPVGDYRLI
                     EVMDRYTSVVGLETPIVLRDGDKRVFRSFASDRDYEFEVNELGWAWGASTISEADVRS
                     IGRIADDRDIFVSVAGEGEKAVPRGGIIDLGGAGFERILTRHHQMPVHTELHVTFVIG
                     GEPVKVAGRPESTLGSLLAKALEETENTGQDVASWQVTDGPGNQLDIAKTLHELGIED
                     GALLIASLRAGAAG"
     gene            902..1504
                     /locus_tag="VFQ54_05335"
     CDS             902..1504
                     /locus_tag="VFQ54_05335"
                     /inference="COORDINATES: protein motif:HMM:NF025811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding protein"
                     /protein_id="HEU0164448.1"
                     /translation="MQVVDPTVSRRKFDREVTQLRDLAPTLTARGIWLMQAEFPVAKV
                     GFATVVARPCVIPFAVRFDFTDYDVQPLGVTFVDPFTDRELMASEMMTKLRRLTSSAP
                     PPPVEIEGGENGPRMVQMTMVELYQHYPQMPQLPGFLCLPGTRAYHAHPAHTGDPWEI
                     HRPSGEGLLFNLVNTIWRYGTAPVVSVMVNFVLGQSVVPE"
     gene            1624..>1880
                     /locus_tag="VFQ54_05340"
     CDS             1624..>1880
                     /locus_tag="VFQ54_05340"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEU0164449.1"
                     /translation="MQEGPRFDIRDAIIPEQKGVSGDHGLMVSVAGGELHRINLLLFE
                     KGWRLLAQIHSHPTHAYHSEMDDEYAIATALGAFSLVVPHFA"
BASE COUNT          368 a          579 c          595 g          338 t
ORIGIN      
        1 cggatagatc attcaaatcc tttaacctga gcgcatccgc gatcctcata gcgaatcgca
       61 tagcagggtc gttccgtcaa taacaacgtt cccagattgg gagcgttgtt gcttgccaaa
      121 atcgaaacgg accggtaacc atgaacttac cgattcgcga gggggacgcg gtccggcggc
      181 gttggcagaa ggatgatggc gacatgatcg aagtcgaaca gaagtgggtc ttcgaggtgg
      241 acggcgtgtc cattgcgacg gacgacagca aactgaaggg tcgcgatgtc cgcacgctcg
      301 ccgggctcgt cccggtgggc gactatcggc tcatcgaggt catggaccga tacacctcgg
      361 tggtcgggct cgaaacgccg attgttttga gggacggcga caagcgcgtg ttccggtcct
      421 tcgcgagcga ccgcgactac gagttcgagg tgaatgaact cggttgggca tggggcgcct
      481 cgacgatctc cgaggcggac gtacggtcga tcggcaggat cgcggacgat cgcgacatct
      541 tcgtctcggt cgcgggcgag ggcgagaagg ccgttccccg cggcggcata atcgatctgg
      601 gcggagctgg cttcgaacgc atcctgacgc ggcaccatca gatgccggtg cacacggaac
      661 tgcatgtcac attcgtgatc ggcggcgagc ccgtcaaagt cgcgggcagg cctgagagca
      721 cgctgggatc gcttttggcg aaggcgctcg aggaaaccga gaacaccggt caggacgttg
      781 ctagctggca ggtgacggac gggcccggaa accagctcga catcgccaag acgctgcacg
      841 agctgggcat cgaggacggc gcgctcctca tagccagcct cagggccgga gccgccggct
      901 gatgcaggtt gtcgacccga cggtcagccg acgcaagttc gaccgcgagg ttacgcagct
      961 gcgcgacctc gcgccgacgt tgacggcacg cggcatctgg ctgatgcagg ccgaatttcc
     1021 ggtggcgaag gtcggcttcg cgacagtcgt cgcaaggcct tgcgtgattc cattcgccgt
     1081 caggttcgac ttcaccgact acgacgtgca gccgctcggc gtcaccttcg ttgatccctt
     1141 cactgatcgc gaactgatgg cgtccgagat gatgaccaag ctccgacgac tgacatcgag
     1201 cgcaccgcct cccccggtcg agatcgaagg cggagagaac ggccctcgaa tggtccagat
     1261 gactatggtg gaactctacc agcactatcc tcaaatgccg cagcttccag gcttcctgtg
     1321 tctcccaggc actcgtgcct accacgctca tccggcacac accggcgacc cctgggaaat
     1381 ccatcgtccg agcggtgaag gactgttgtt caacctcgtg aacacgatct ggcgatacgg
     1441 caccgcgccc gtcgtcagcg tgatggtcaa tttcgtcctc ggacaatccg tagtgcccga
     1501 atgagagcct ccctcactga ggtcacctcg gtccaccttc ccgtcggcat ggcgatgaag
     1561 acccatgccc acctacggag cgcaggcctc cgtggtctcg aaggcatggc gctatgggtt
     1621 ggggtgcagg agggccctcg gttcgatatc cgggacgcga tcatccccga gcagaagggc
     1681 gtcagcggcg atcacggtct gatggtatcg gtcgcaggcg gcgagttgca ccgcatcaac
     1741 ctcctgctct tcgagaaggg atggcggctg ctcgcccaga tccacagtca tcccacgcac
     1801 gcctaccaca gcgagatgga cgacgaatac gcaatcgcga ccgcgctcgg cgcattctcg
     1861 ctcgtggtgc cgcatttcgc
//
LOCUS       DASTMD010000364         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1731143, whole genome shotgun sequence.
ACCESSION   DASTMD010000364 DASTMD010000000
VERSION     DASTMD010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731143"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..999)
                     /locus_tag="VFQ54_05345"
     CDS             complement(37..999)
                     /locus_tag="VFQ54_05345"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEU0164450.1"
                     /translation="MHSLPFDKPGRFFKGNLHTHSTRSDGGLTPAEVTTAYRERGYDF
                     LALTDHFQPETYFRKTADPASFVAVTDTREFRTDDFTTILGAEVHGPGMANGETWHIL
                     AAGLPLDFAPWAAGETGLEVAKRAVAAGAFVAIAHPRWNSLSVEDAREAAKIAHAVEV
                     YNQACSSEIDRGDSWYMADLLAQEGFRLSACATDDAHIEFPLYPNTWTDDAFGGWVHV
                     RAEALEQDALVAALKAGQYYSSTGPEIHHVSIEDDTLIVECDPAVQIFATGNGALNQR
                     SRGGSVIQAAFPLARFRKAGFVRVTVVGEKGGRAWTNPIWLDAE"
     gene            1167..1481
                     /locus_tag="VFQ54_05350"
     CDS             1167..1481
                     /locus_tag="VFQ54_05350"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU0164451.1"
                     /translation="MAKTFYASYIEFVPVKGETDKLLAAMLEAATAMENVPDCLQYAV
                     GLNAENRNIGVWEVWTSQDAHERSLMDAETRAFIKQTMPSIKEIIPARQIDLRGGKGV
                     PS"
     gene            1600..2283
                     /locus_tag="VFQ54_05355"
     CDS             1600..2283
                     /locus_tag="VFQ54_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164452.1"
                     /translation="MLTSIVLTAGGLGLAGIDPSGAILGMGLFGAGAPRRNILLMVAI
                     YLLGTIAIGVTLSIVFAGRIATLNITELEPPDWVDAIIFAVAGILLLAWGGLRVIHPA
                     PPRTEDRRRGTGMKALALLGVVLAAGSLLSPPFIAMIVVAGKYHAIWAILLGHTIWTL
                     INQSPLLLVTGVEMTGRGDRIVTAAHDWWIREQPAIARIFTTLLVLVSVALLLESAWW
                     TVARDFLLI"
     gene            2298..>2663
                     /locus_tag="VFQ54_05360"
     CDS             2298..>2663
                     /locus_tag="VFQ54_05360"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HEU0164453.1"
                     /translation="MSEVLPLTLAGYAHRRDALLARIVEVLEADPRVVAAWLSGAHGR
                     GVADAWSDLDLHVAVEDAALAAFLDERDDFYRRFGEPVLVQEEMPSNAMPASRFQLVI
                     YAGPVEVDWTIGPLREETRP"
BASE COUNT          420 a          806 c          949 g          488 t
ORIGIN      
        1 atgcggtggc gcgggtgttc caccatcaat cggggatcac tccgcgtcga gccagatcgg
       61 gttcgtccag gcgcgaccac ccttctcgcc gacgacggtg acgcggacga agccggcctt
      121 gcggaaccgt gcgagcggga acgcggcctg gatcacgctg ccaccacgcg accgctggtt
      181 cagcgcgccg ttgccggtcg cgaagatctg gacggcagga tcgcattcga cgatcagggt
      241 gtcgtcctcg atcgagacgt gatggatttc cggtcccgtc gacgagtagt actgacccgc
      301 cttcagggcc gctacgagcg cgtcctgctc gagtgcttcc gcccggacgt gaacccagcc
      361 gccgaaggcg tcatccgtcc atgtattcgg atagagcggg aactcgatgt gggcgtcgtc
      421 ggtcgcgcag gcggagagtc ggaagccctc ctgcgccagc aggtccgcca tgtaccagct
      481 gtcgccacga tcgatctcgc tggagcaggc ctggttgtag acctcgacgg catgggcgat
      541 tttcgccgct tcgcgggcgt cctcgacgga gagcgagttc cagcggggat gggcgatcgc
      601 gacgaaggct ccggcggcga ccgcacgctt cgcgacctcg agacccgtct cacccgcggc
      661 ccatggcgcg aaatccaggg ggagaccggc ggccaggatg tgccaggtct cgccgtttgc
      721 catcccggga ccgtggacct cggcgccgag gatcgtcgtg aagtcgtcgg tccggaactc
      781 ccgcgtgtcg gtgaccgcga cgaagctcgc cggatcggcg gtcttccgga aataggtctc
      841 cggctggaag tgatcggtca gggcaaggaa gtcgtagcca cgctcgcgat aggcggtcgt
      901 gacctcggcg ggcgtgagac cgccgtcgga acgcgtcgaa tgggtgtgaa ggtttccctt
      961 gaagaagcga cccggcttgt cgaatggcag gctatgcatc ggtggcaatc cccggtgatc
     1021 gtcgtggtga tgtcatccat aaggagcggt ggcaaacgct ggcagcctac ggtacgtccc
     1081 gcggccttgc aagcgaggta tcgcggattg gtatgatcgg atcgccggag agtgcgtgcg
     1141 atcgacgaga tggagtgatg aaacccatgg cgaagacctt ctacgcgtcc tacattgaat
     1201 tcgttcccgt gaagggtgag acggacaagc tgctggcggc gatgctcgag gcggcgaccg
     1261 cgatggagaa tgtcccggat tgcctgcagt atgcggtggg gttgaatgcc gagaacagga
     1321 acatcggcgt ctgggaagtc tggacgagcc aggatgccca cgagcgttcg ttgatggacg
     1381 cggagacgcg ggcgttcatc aagcagacga tgccgtcgat caaggagatc atcccggcgc
     1441 ggcagatcga cctgcgcggt ggcaagggag tgccctcgta gcacgcgttc ggaccgctgt
     1501 gccgcgccgt ttcgttcgtg tgagggtgga gtcgctcctg cattcgtcgt cgcggtgacg
     1561 gcggctccgg agctctgggg atttcgggcg ggcggggaga tgttgacgag catcgtgctg
     1621 acggcgggag ggctgggcct ggccgggatc gatccctccg gggcgatcct ggggatgggt
     1681 ctcttcggcg cgggcgcgcc gcgccgcaat atcctgctga tggtcgcaat ctacctgctc
     1741 ggcacgatcg cgatcggcgt cacgctgtcg attgtcttcg ccggtcgtat cgcgacgctg
     1801 aacatcaccg agctcgagcc ccccgactgg gtcgacgcga tcatcttcgc tgtcgcggga
     1861 atcctgcttc tcgcctgggg cgggctccgc gtgatccatc cggcgccccc gcgtacggag
     1921 gaccgtcgcc ggggaaccgg gatgaaggcg ctggcgctgc tcggggtggt gctcgcggcc
     1981 ggctcgctgc tctcgccgcc cttcatcgcg atgatcgtcg tcgccgggaa gtatcacgcg
     2041 atctgggcga tccttctcgg gcacacgatc tggacgctga tcaaccagtc cccgctgctg
     2101 ctcgtgaccg gggtggagat gaccggtcgc ggcgatcgga tcgtgactgc ggcgcacgac
     2161 tggtggatcc gggagcagcc ggcgatcgcg cggatcttca ccacgctgct ggtgctcgtc
     2221 agcgtcgccc tgcttctcga gagcgcgtgg tggacggtcg cccgcgactt cctgctgatc
     2281 tgatctggag ggaacagatg tccgaggtcc tccccctgac acttgccgga tacgcgcatc
     2341 gacgcgatgc cttgctcgcc cgaatcgtcg aggtactcga ggcggatccg cgggtcgtcg
     2401 cggcgtggct ctccggcgcg catgggcgcg gcgtcgccga tgcctggagc gacctcgatc
     2461 tccacgtcgc ggtggaagat gctgccctcg cggcatttct ggatgaacgt gacgacttct
     2521 atcggcgttt tggcgaaccg gtgctggtcc aggaggagat gccatcgaat gcgatgccgg
     2581 cgagccggtt ccagttggtg atctatgctg ggccggtcga ggtggactgg acgatcggac
     2641 cactcagaga ggaaacgcgt ccg
//
LOCUS       DASTMD010000365         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1373706, whole genome shotgun sequence.
ACCESSION   DASTMD010000365 DASTMD010000000
VERSION     DASTMD010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373706"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1151
                     /locus_tag="VFQ54_05365"
     CDS             114..1151
                     /locus_tag="VFQ54_05365"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEU0164454.1"
                     /translation="MVFDRAARLDLLATHAAGYLDVALANIVREYPHMPWFIAESETD
                     YRTHRIYHPAFFGCLDWHSCVEMHWAMIRILRLFPDAAGADRVRRQLDDLLTDENLAT
                     EKAFMDANPGFERPYGRGWYLTLQHELATWDDADGQRWATILQPLADSIAEGLVAWLP
                     KLPYPVRIGMHQNTAFGLSRALDFAARRADHGSPDLLDAIHSAARHWFLEDVDYPGNY
                     EPSGSDFLSGGLCEAELVSSLLPTDEFSQWLGRFLPGISDREPRSLFSPATVTDHADG
                     QIAHLEGLNLSRAWNFIRIAAALPEDDPRAAPLIDAAGYHAQASLGAVAGSDYMVEHW
                     LAAYATLLLSS"
     gene            1305..>1530
                     /locus_tag="VFQ54_05370"
     CDS             1305..>1530
                     /locus_tag="VFQ54_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164455.1"
                     /translation="MLYDHVVAVVDDARLSAILPVIHRSGLGHVARALRANRVDVREQ
                     LRRAGVPVEQSPVELPDARLLLMIGAAARSP"
BASE COUNT          253 a          554 c          445 g          278 t
ORIGIN      
        1 tccggactgc ggtccggcag cggcggtccc gccctacgaa cgaccggatg ccgccgcgac
       61 gcgtggtaga ctcgcgatct tgtctttcgg ccgaacctgc agggaggaat ctgatggtct
      121 tcgatcgcgc agcgcgactc gatctgctcg ccacgcacgc cgccggctac ctcgatgtcg
      181 cgctggccaa catcgtccgc gagtacccgc acatgccctg gttcatcgcc gaatcggaaa
      241 cggactaccg gacgcaccgc atctatcatc cggccttctt cggctgcctc gactggcatt
      301 cctgcgtcga gatgcattgg gcgatgatcc gcatcctccg cctctttccg gacgcagccg
      361 gcgcagatcg cgtccgccgc cagctcgacg acctgctcac cgacgaaaat ctggcgaccg
      421 agaaagcatt catggacgcc aatcccggat tcgaacggcc gtacgggcgt gggtggtacc
      481 tgacactcca gcacgaactc gccacctggg atgacgcgga cggccagcgc tgggcgacga
      541 tcctccagcc gctcgccgac tcgatcgcgg aggggctcgt cgcctggctg ccgaagctcc
      601 cctatcccgt ccggatcggc atgcaccaga acacggcctt cggcctctca cgcgcgctcg
      661 acttcgcggc acgacgcgcc gaccatggaa gcccggatct gctcgatgcg atccacagcg
      721 cggcgcggca ttggttcctc gaggatgtcg attacccggg aaactacgag ccgtcgggca
      781 gtgacttcct ctccggcggt ctctgcgaag cggaactcgt ctccagcctg ttgccgacgg
      841 atgagttctc gcaatggctc ggccgtttcc tgcccggtat ctccgatcgc gaaccgcggt
      901 cgctcttctc gccggcaacc gtcaccgacc acgccgacgg ccagatcgcc catctcgagg
      961 gcctcaacct gagtcgcgca tggaacttca tccggattgc ggcggcactg ccggaggacg
     1021 atcctcgcgc cgcaccactc atcgacgccg cggggtatca cgcccaggcg tcacttggcg
     1081 ctgtcgctgg cagcgactac atggtcgagc actggctggc cgcgtacgcg acgcttctcc
     1141 tctccagttg acctcaggac aagacttccg gcagcgtcga ccaaaatccc ggcgggatat
     1201 atccgaacga acgtgctatc ctgtgccagc acatgtgaca ctaaggcaca tgatgaccgt
     1261 tcggtcgtaa cgcctcaccc gcgttctccc cgggacctcc aatcatgctc tacgatcatg
     1321 tcgttgctgt tgtcgacgac gcccgcctct cagctatcct gccggtcatc caccgcagcg
     1381 gactcggtca cgttgctcgc gcgctccgtg cgaatcgtgt cgacgtccgc gagcaactcc
     1441 gtcgcgccgg cgttcccgtc gaacagagcc cggtcgagtt gccggatgcc cgtcttctcc
     1501 tcatgatcgg cgccgcggcg cgaagtccgg
//
LOCUS       DASTMD010000366         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2444891, whole genome shotgun sequence.
ACCESSION   DASTMD010000366 DASTMD010000000
VERSION     DASTMD010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2444891"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFQ54_05375"
     CDS             complement(<1..204)
                     /locus_tag="VFQ54_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164456.1"
                     /translation="MMSRFSIVSRHTVLILLALLALFPLAFLLINSVKTELAYSRDPL
                     ALPDAFQWDQYRKAFDLIIRPTMN"
     gene            complement(254..1183)
                     /locus_tag="VFQ54_05380"
     CDS             complement(254..1183)
                     /locus_tag="VFQ54_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0164457.1"
                     /translation="MATVGPIIAETGRRGIFTARTRDRARRHLAAYLFLVPTFAFIGY
                     FLYYPAFRALIGGFTYWDGFNPPTYAGLDNFRRAFNDQILETATKNNLKWAAIEITLS
                     ILPAFLVAELIFHMQRDRVRYLYRTLFVVPIIIPSIVSILLWRYYYQGDGLINDILGR
                     VGLNGLTHFWLSDPNTALYALALMGFPWVNAFNMLIFFAGLQNIPTEVMEAAELDGAT
                     GLRRTWTIEIPMVLSQFKLLIILAIITSGQNIVTPLVMTQGGPGYATYTVSYYMYDTA
                     VDYGEFGYSMAIALLLFIVILVLTAINQRFIRD"
     gene            complement(1294..>2424)
                     /locus_tag="VFQ54_05385"
     CDS             complement(1294..>2424)
                     /locus_tag="VFQ54_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164458.1"
                     /translation="NGTLPKGIARDLKADFAKPNPYIKDKPTWFDAMNPTVQAETVAP
                     SGAVYNLNGDFVGTAFFYNKGLFDKAGITAAPTTWTELFAACQKLADAGITVCQGFAD
                     PSWFGRHFLSDFYSTDYDKIIGCDGSPGESPQDEAASIKSGLLSTDDPRFMGWWPFFK
                     KFTDFWSQEYLTQTTTVATEPVQRDFAAGKTAILYTGSWLPRTLKTIGYDFELSSFSF
                     PKLAKTDIEFATDTDVAAVVGGPNAAYQFAMATKDSNKSIGDGGKEAAVLDFLQYIGT
                     PAVIEKVVNELGSFAPTWPGTKPVAGLETFVAQANSGLKVVGIGSTSAKLGPNLERIF
                     GLYLTGNTSDSDASKQVQTELDNAVKDYERTNPDINLDSCAG"
BASE COUNT          474 a          685 c          794 g          471 t
ORIGIN      
        1 gttcatcgtc ggccggatga tgagatcgaa ggccttgcgg tattgatccc attggaatgc
       61 gtccggcaag gcaagtggat cgcgcgaata ggcgagttcc gtcttcaccg agttgatgag
      121 gagaaacgcg aggggaaaga gcgccagcag cgcgagaagg atcaacaccg tatgccgtga
      181 aacgatcgag aagcgtgaca tcatcctgtg ccctccggta tggctgcgtt gccgtctgaa
      241 aacgtgcggc cggtcaatcc cggatgaagc gctgattgat cgcggtgagg acgaggatca
      301 cgatgaagag caatagggcg atcgccatgc tgtagccgaa ttccccgtaa tcgaccgcgg
      361 tgtcatacat gtagtaggag acggtgtagg tggcgtagcc cggcccgccc tgggtcatca
      421 ccaatggcgt cacgatgttc tggccactcg tgatgatcgc gaggatgatg agcagcttga
      481 actgggagag gaccatcggg atctcgatcg tccacgtccg gcgcaatccg gtcgcgccat
      541 cgagttccgc cgcttccatc acctcggtcg ggatgttctg cagcccggcg aagaagatga
      601 gcatgttgaa ggcgttgacc cacgggaagc ccatcagcgc cagcgcgtag agcgccgtgt
      661 tcggatccga gagccagaag tgggtcaggc cgttgagtcc gacgcgcccc aggatgtcgt
      721 tgatcagccc gtcaccctgg tagtagtagc gccagagcag aatcgagacg atcgacggga
      781 tgatgatggg gacgacgaaa agcgtgcgat agagatagcg cacgcgatcc cgctgcatgt
      841 gaaagatcag ctcggcgacg aggaacgcgg gcaggatcga gagtgtgatc tcgatcgcgg
      901 cccacttcag gttgttcttg gtcgcggtct cgaggatctg gtcgttgaac gcgcgccgga
      961 agttgtcgag accggcgtag gttggtggat tgaacccgtc ccaataggtg aatccgccga
     1021 tcagcgcgcg gaatgccggg tagtagagga agtacccgat gaaggcgaac gtcggaacga
     1081 gaaagagata ggccgccaga tgacgtcgtg cacgatcgcg tgtgcgtgcc gtgaagattc
     1141 cccggcgacc agtctccgcg atgattggcc ctacggtcgc catgatctgt ccctcgcatg
     1201 cttcccggaa accggtgacg agcggaacga cgcatcgttc cactcgtcac ggcgatcccg
     1261 tcaattcggc actgattccc ccggatgggc cggctagccg gcgcaggagt ccaggttgat
     1321 atccggattg gttcgctcgt aatccttcac ggcgttgtcg agctcggtct gcacctgctt
     1381 cgaggcatcg ctgtcgctgg tgttcccggt gaggtagagc ccgaagatgc gttcgaggtt
     1441 cggcccgagc ttcgcgctgg tgctgccgat gccgaccacc ttgagaccgg aattcgcctg
     1501 ggcgacgaac gtctcgagtc cggcgaccgg cttcgtgccg ggccaggtcg gggcaaagct
     1561 cccgagttcg ttgaccacct tttcgatcac ggccggggtt ccgatgtact ggaggaagtc
     1621 gagcacggcc gcttccttgc cgccatcgcc gatcgacttg ttcgagtcct tggtcgccat
     1681 cgcaaactgg taggccgcat tcgggccacc caccacggcc gcgacatcgg tgtcggtcgc
     1741 gaactcgatg tccgtcttcg caagtttcgg gaagctgaac gaactcagct cgaagtcgta
     1801 gccgatcgtc ttcagggtcc ggggaagcca ggaaccggta tagagaatgg ccgtcttacc
     1861 ggcggcgaaa tcgcgttgca ccggttcggt cgcgaccgtt gtggtctggg tcaggtattc
     1921 ctgggaccag aagtcggtga acttcttgaa gaacggccac cagcccatga atcggggatc
     1981 atcggtcgaa agcaacccgc tcttgatcga ggcagcttca tcctgcgggg actcgccggg
     2041 agaaccgtcg catccgatga tcttgtcgta atccgtggag tagaagtcgg agaggaagtg
     2101 ccggccgaac cacgacggat cggcgaagcc ctggcagacc gtgatccccg cgtcggcgag
     2161 cttctggcag gccgcgaaga gttccgtcca cgtcgtcggg gcggcggtga tccccgcctt
     2221 gtcgaagagc cccttgttgt agaagaaggc ggtaccgacg aagtccccgt tcaggttgta
     2281 gaccgcaccg cttggcgcga ccgtttccgc ctggaccgtg ggattcatcg cgtcgaacca
     2341 ggttggcttg tccttgatat acggattcgg cttcgcgaaa tctgccttga gatcacgcgc
     2401 gatccccttc ggcagggtgc catt
//
LOCUS       DASTMD010000367         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_213541, whole genome shotgun sequence.
ACCESSION   DASTMD010000367 DASTMD010000000
VERSION     DASTMD010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213541"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1673
                     /locus_tag="VFQ54_05390"
     CDS             192..1673
                     /locus_tag="VFQ54_05390"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEU0164459.1"
                     /translation="MAETRYRIGIGGIAIESSTFSPLPSTIEDFVIQRGDEIVAGYPY
                     FENGTFEGRDDVEWFGTLKARAIPGGPVTSESYARMKGELIERLHAALPLDGFFYDIH
                     GAMSVIGLDDAEGDLITAIRETVGPACIISTGMDLHGNVSDVLVGAIDCFTAYRQAPH
                     VDAMETKARAVRNLLNLLDEGVRPFRAWTRIPVNFPGEKTSTMWEPGATVYKRLEESD
                     GIPGLIDASLWVGYVWADQPRANATTVVTGTDVDVIRAEAEKIAKRYWDAREEYRFGV
                     PTGTADWTIDEALKLNQKAVLISDSGDNPTAGGAGDIPYMVERLLANPALASGERTAM
                     VSAIASPEAVKIAKAAGIGGEVDVVIGGINDPVNGSPFNLHGVVYSIYENDPVGGDIV
                     VIRSGGVHVVIPTKRKPYHHLKEFANINLAIPDFDITVGKWGYLEPELKGAASAAFQA
                     LTPGAVNQDIESLPFKHVDRPVFPLDRDMPEPDWEIRIFPPIA"
     gene            complement(1806..>3086)
                     /locus_tag="VFQ54_05395"
     CDS             complement(1806..>3086)
                     /locus_tag="VFQ54_05395"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU0164460.1"
                     /translation="DWPITYDELEPYYDKVEYLIGVSGKGGANPFESPRSRDFPLPPL
                     REMGYATLAKDAMTKLGYHPFPQPTAILSEAYDGRPACTFCGFCGSYGCWNDSKSSTL
                     VSAIRHGEATGNLEIRPNSRVMKILSNDKGQVTGVQYLDDKGELQEQPAGVVILSTYI
                     YENNRLLLVSTSDFYKKGLANNGGNVGKHYMSHVYTGNYGFFPKHRLNLFSGSNGQAI
                     AMDDLNGDNFDHTGLGFVRGGVLFASNGNLPIANSGTLPPSAPTWGAAYKKFLHDYGD
                     SSAQVFAQVEPLPYESNFVDLDPKMKDPLGMPVVRVTYSLQQNEIKAQTYISAKINDI
                     LKQMGATEFWPSFPVGLAIPINSHAYGGTRMGDDPDTSVVDKYGLAHEAPNLMVLGGS
                     NFPGVSGYNPTETIEAHSWFAADYLAKNLDKVAI"
BASE COUNT          540 a          914 c         1057 g          575 t
ORIGIN      
        1 tggcgggagc ttggcgcgcg tgaccgggtg atcattccgg cggacgggga gcttgccctc
       61 gcctcctgac gccgcgccgg cggccgggac gacggcccgt cttgcgtgga tgtcgaggct
      121 cggctaaggt cgctacggtg gatttcgacg ggaatggccg gcgtatcgcg acaatcaagg
      181 aatgacatct cgtggcagag accagataca ggatcggcat cggtggcatt gcaatcgaga
      241 gcagcacctt ctcgccgctg ccctcgacga tcgaggattt cgtgatccag cgcggtgacg
      301 agatcgtcgc cggctaccca tacttcgaga atggcacctt cgagggtcgg gacgatgtcg
      361 agtggttcgg cacgctgaag gcgcgcgcga tcccgggcgg accggtcacc agcgaaagct
      421 acgcccggat gaagggcgag ctgatcgagc gcctgcacgc cgccctgcct cttgacgggt
      481 tcttctacga tatccacggc gcgatgtcag tgatcggact cgacgacgcc gagggtgacc
      541 tgatcacggc gatccgggag acggttggtc cggcctgcat catctcgacc gggatggatc
      601 tgcacggcaa cgtctccgac gttctcgtcg gcgcgatcga ctgcttcacc gcctaccgcc
      661 aggcgccgca cgtcgacgcg atggagacga aggcgcgcgc cgtgcgcaat ctgctcaacc
      721 tcctcgacga gggagtgcga ccgttccgcg cctggacgcg gattccggtc aacttccccg
      781 gcgagaagac gagcacgatg tgggagccgg gcgcgaccgt ctacaagcga ctcgaggagt
      841 ccgacggcat cccgggcctg atcgatgcct cgctctgggt ggggtacgtc tgggccgacc
      901 agccacgagc gaatgcgacg acggtcgtca ccggtaccga tgtcgacgtg atccgggccg
      961 aggcggagaa gatcgcgaag cgctactggg atgcgcgcga ggaataccgc ttcggcgtcc
     1021 cgaccggcac cgccgactgg acgatcgatg aggcgctcaa gctcaaccag aaggccgtgc
     1081 tgatcagtga ctcgggtgat aacccgacgg ccggtggcgc cggcgatatt ccgtacatgg
     1141 tcgagcgatt gcttgcgaac ccggcgctgg cgtccggcga gcggacggcg atggtctcgg
     1201 cgatcgcgag cccggaggcg gtgaagatcg cgaaggcggc gggtatcggc ggcgaggtcg
     1261 atgtcgtgat cggtggcatc aacgatccgg tcaacggctc gcccttcaat ctccacggcg
     1321 tcgtgtattc gatctatgag aacgatccgg tcggtggcga catcgtggtg atccgcagcg
     1381 gtggcgttca cgtcgtgatc ccgacgaagc gcaagccgta tcaccatctc aaggaattcg
     1441 cgaacatcaa cctcgcgatt ccggacttcg atatcacggt tggcaagtgg gggtatctcg
     1501 agccggagct gaagggcgcc gcctccgccg cgttccaggc actgacgccg ggtgcggtca
     1561 accaggacat cgagtcgctg ccgttcaagc acgtcgatcg cccggtcttt ccgctcgatc
     1621 gtgacatgcc ggagccggat tgggaaatcc gtatcttccc gccgatcgcc tgaccggaat
     1681 gttggggcgg gacagtgaag tcccgccctt cgaacgcgca ctgagggaat ggccgtcgat
     1741 aaagcggtcg tgagtacggg gggacttcta tgtcccccct tttgggtcgc cttcttcccg
     1801 atcgatcaga tcgcgacctt gtcgaggttc ttggcgagat agtcggccgc gaaccaggaa
     1861 tgggcctcga tcgtttcggt cgggttgtat ccggagacgc cggggaagtt cgagccgccg
     1921 aggaccatca ggttgggcgc ctcgtgagcg agcccgtact tgtccacgac cgaggtatcc
     1981 ggatcgtctc ccatgcgcgt gccaccgtag gcgtggctgt tgatcgggat cgcgagaccg
     2041 accgggaagc tcggccagaa ttccgtcgcg cccatctgct tcaggatgtc gttgatcttg
     2101 gccgagatgt aggtctgcgc cttgatctca ttctgctgga gactgtaggt gacgcgcacg
     2161 accggcatcc cgaggggatc cttcatcttg ggatcgaggt cgacgaagtt gctctcgtag
     2221 ggaagcggtt cgacctgcgc gaagacctgc gccgacgagt cgccatagtc gtgcaggaac
     2281 ttcttgtacg ccgcacccca ggtcggcgcg ctcggcggca gggtgcccga attcgcgatc
     2341 gggaggttcc cgttgctggc gaagaggacg ccgccgcgga cgaatccgag accggtgtga
     2401 tcgaagttgt cgccgttgag atcgtccatt gcgatcgcct ggccgttgga gccggagaag
     2461 agattcaggc ggtgcttggg gaagaagccg tagttcccgg tgtagacgtg ggacatgtag
     2521 tgcttgccga cgttgccacc gttgttggcg agccccttct tgtagaagtc cgaggtagag
     2581 acgaggagca ggcggttgtt ctcgtagatg taggtcgaga ggatgaccac accggccggc
     2641 tgctcctgca gctcgccctt gtcgtcgaga tactggacgc cggtgacctg gcccttgtcg
     2701 ttggagagga tcttcatgac ccggctgttg ggccggatct cgagattgcc ggtcgcctca
     2761 ccgtgccgga tcgcggagac gagcgtgctc gacttcgaat cgttccagca gccgtaactt
     2821 ccacagaagc cgcagaaggt gcaggccggg cggccgtcgt acgcctcgct gaggatggcg
     2881 gtcggctggg ggaagggatg gtagccgagc ttcgtcatcg catccttcgc cagggtggca
     2941 tagcccatct ctcgcaacgg tggaagcgga aagtcgcgac tacgcggcga ttcgaacgga
     3001 ttcgcaccgc ccttgccgga gacgccgatc aggtactcga ccttgtcgta gtagggctcg
     3061 agctcatcgt aggtgatcgg ccagtc
//
LOCUS       DASTMD010000368         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_122547, whole genome shotgun sequence.
ACCESSION   DASTMD010000368 DASTMD010000000
VERSION     DASTMD010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122547"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2483
                     /locus_tag="VFQ54_05400"
     CDS             <1..2483
                     /locus_tag="VFQ54_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164461.1"
                     /translation="TFEDGFTDLGKRMSARMRENGTLSGRSFTDALEDVINHDKGRLA
                     NDLADIFGNTDGLDRYFDKFENVDTAVDSLRTDIGRLSDAGGVSNEEFLLMAGALDKY
                     ADRARVTEGENHRVRDSLADLRLELERGADNASRVEEAFRGVDGENKTLRKSFSDLRD
                     MVDDVNVRLGSRSAFNDLAGRIGGSNNALNLFHDVLGRARSAGVLADREFFKMNDNLD
                     VLAKRLDPIDDAFDKATKSSNGFAGGISTDAKQIIAIVAIIAGAAPQILSLGSFIASS
                     VVVGVTALAALGAGIAVAVAGFIELSGSINDLPKAVQPAAKAFQGLLKPLQALQDAIT
                     VGLFSNLTSGFESLGTLITNLTGPIQQFSAQVGQAIGQIITAVTSPAFTNGFTAFLAA
                     AGPIFSTLTQAAISFGGAIGNILVDALPYAQKFADGIAKIGDAFSDWTKSQAGRDAIT
                     KFFEHGQQIAGPLLNLVGKLGDMFGRLVTDQTVAATATFLDTVTQILPFIESLIGVLN
                     QANVFQVVADILSQIGNALIPILNIISPILDVVLTLIEDAIRPLGAVLQLVALVLEPV
                     KVGFEIFNAILQALQPYIISFQSVANDLFSALQTGADTVLSALKPAFDELLGSIINLL
                     PSPQALHDIIVNQVVPAIQSATDWIVKHLIPAVVDIVKHVSEFIDSLGGFDGIEKKLE
                     GAWNAFKTFGDIVSAALTPVKLTIDAIMAGIRFLNGTKINVGGIGANGSANPLLHAAG
                     GILNEPTFLTPRDIAGEAGAEAIVPLQRPLSQISPEVRPLAAFAQGKTGGDTTVHNWE
                     PGSVTVVTPAEDPEMVAETVVDNIIAGL"
BASE COUNT          497 a          892 c          771 g          473 t
ORIGIN      
        1 acaccttcga ggatggcttc accgatctcg gcaagcggat gtccgcgcgg atgcgagaga
       61 acgggaccct ctcgggtcgc agcttcaccg acgccctcga ggacgtgatc aatcacgaca
      121 agggccgcct ggccaacgac ctcgctgaca tcttcggcaa cactgatggc ctcgaccgct
      181 acttcgacaa gttcgagaac gtcgacaccg cagtcgattc cctgcgcacc gacatcggtc
      241 ggctctcgga tgccggcggc gtctcgaacg aggagttcct gctcatggcc ggagctctgg
      301 acaaatacgc ggaccgcgcc cgggtgacag aaggcgagaa ccatcgtgtc cgcgactccc
      361 tcgcggacct gcggctcgag ctggagcgcg gcgccgacaa tgccagccgc gtcgaggagg
      421 cgttccgggg tgtcgacggc gagaacaaga ctctgcgcaa gtccttctcc gatctgcggg
      481 acatggtcga cgacgtcaac gtgcggctcg gcagtcggtc ggccttcaac gatctggccg
      541 ggcgcatcgg tggatcgaac aacgcactga acctgttcca cgatgtcctc gggcgcgctc
      601 gcagtgcggg ggtgcttgcg gatcgtgagt tcttcaagat gaacgacaac ctggacgtgc
      661 tggccaagag gctcgacccg atcgacgacg ccttcgacaa ggcgaccaag tcgtcgaacg
      721 ggttcgccgg cgggatctcc accgacgcca agcagatcat cgcgatcgtc gccatcatcg
      781 cgggcgccgc tccgcagatc ctctcccttg gctccttcat cgcctcctcg gtggttgtcg
      841 gcgtgacggc tcttgccgcg ctgggcgccg ggatcgctgt ggccgtagcg ggattcatcg
      901 aactgtcggg gtccatcaac gatctcccga aggcggtaca gcctgcggcc aaggcgttcc
      961 agggtctact caagccactg caggcactgc aggatgccat aacggttggg cttttctcga
     1021 acctcacgag tgggttcgag tcactgggca cgctcatcac gaacctgacg ggaccgatcc
     1081 agcagttctc cgcacaggtg ggtcaggcga tcgggcagat catcaccgcc gtgacgtcgc
     1141 cggccttcac caatggattc accgcgttcc tcgctgcggc cggtccgatt ttctccaccc
     1201 tcacccaggc cgcgatcagc ttcggtggcg ccatcggcaa catccttgtc gacgccctcc
     1261 cctatgccca gaagttcgcc gacggaatcg cgaagatcgg ggacgccttc tccgactgga
     1321 ccaagtcgca ggcgggtcga gatgcgatca cgaagttctt cgagcacggg cagcagatcg
     1381 caggccccct cctcaacctg gtgggcaagc tgggtgacat gttcgggcgc ctagtcaccg
     1441 accagaccgt cgcggcaaca gcaaccttcc tcgacacggt gacccagatc ctgcccttca
     1501 tcgagagcct catcggcgtc ctgaatcagg ccaacgtctt ccaggtggtg gccgacatcc
     1561 tgtcccagat cggcaatgcg ctgatcccga tcctgaacat catctccccc atcctcgatg
     1621 tggtccttac cctgatcgag gatgccatac gccctctcgg cgccgtcctg cagctcgtcg
     1681 cactcgtgct cgagccagtg aaggtcgggt tcgagatctt caacgccatc ctgcaggcgc
     1741 tgcagccgta catcatctcc ttccagtccg tagccaatga tcttttctcg gcgctgcaga
     1801 ccggagccga cactgtcctc tcggccctca agccggcctt cgacgaactg ctcggctcga
     1861 tcatcaatct ccttccgagc ccacaagcgc ttcacgacat catcgtcaac caggtggtcc
     1921 cggccatcca gagcgcgacg gattggatcg tcaagcacct gattccagcc gtggtcgaca
     1981 tagtcaagca tgtctctgag ttcatcgaca gcctcggtgg attcgacgga atcgagaaga
     2041 agctcgaggg ggcctggaac gccttcaaga ccttcggcga catcgtctcg gcagccctga
     2101 cccccgtgaa gctcaccatc gacgccatca tggcgggcat ccgattcctg aacggcacca
     2161 agatcaacgt gggcgggatc ggcgcgaacg gcagcgccaa cccgcttctc cacgcggccg
     2221 ggggaatcct gaacgagccc acgttcctca ccccacggga tatcgccggc gaggctggag
     2281 ccgaggccat cgtcccactg cagcgaccgc tcagccagat cagtccagag gtgcgcccac
     2341 tggccgcctt cgcccagggc aagaccggtg gcgacaccac ggtgcacaac tgggagcctg
     2401 gttctgttac cgtcgtgaca ccggcagaag atcctgagat ggtcgcagag accgtcgtcg
     2461 acaacatcat cgctggcctg tagttaggag agattgtggc cttcgacgga ttcctcgagt
     2521 atgcaggcgt cgagatcatc aacgccgagc gcacgcagaa ctacattcgc caccaggccc
     2581 cctggatctc gctgcccacc ctggagaagt acgaggggct gcacctcgcg ctc
//
LOCUS       DASTMD010000369         4503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1821728, whole genome shotgun sequence.
ACCESSION   DASTMD010000369 DASTMD010000000
VERSION     DASTMD010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821728"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFQ54_05405"
     CDS             complement(<1..235)
                     /locus_tag="VFQ54_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164462.1"
                     /translation="MDRLLHRLYDSTTHHSHLRVIIRETIIIGSFAFFYFYVRGLMDA
                     KRTLAMAHEVSIIHFEQHLGIFVEPDLQSWIRHR"
     gene            409..1104
                     /locus_tag="VFQ54_05410"
     CDS             409..1104
                     /locus_tag="VFQ54_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164463.1"
                     /translation="MSDQERSETDIEAPDHDAAKDVDATIEREVAADEAPIAAPPAGA
                     AAEADPASDADDLDAAPDTADDDETGVKRVSSSAVQNIDAHQVVVESSAVQSIKAQRV
                     SLANSGAKSIDARSVKMEKSGTAILRSERATLNDSSIVIGMTSEFALNQSSAGIVMSG
                     ETKIAGDSRVGFLTTGTVDATGDIRSVLLTAGTVKAGGTVTTVVNPVSGAAIGAGFAF
                     VLLIVRFVFRRGK"
     gene            1142..1393
                     /locus_tag="VFQ54_05415"
     CDS             1142..1393
                     /locus_tag="VFQ54_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU0164464.1"
                     /translation="MEGIKRLFNFGLGGAIGTAVGVIVGSLLAPQSGKELQHSTQRFV
                     DEVKAGGDAAQAETEAQIRARFRNRVGDPTAMTETSAKA"
     gene            1444..2412
                     /locus_tag="VFQ54_05420"
     CDS             1444..2412
                     /locus_tag="VFQ54_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0164465.1"
                     /translation="MEHVQPAAIVPGFHHSNQTASTKRGTRYHETTAKSIVNAVKGMR
                     FSWSISPYRGCIHACTYCYGRASHSFLGYDTGADFEHEIIVKTNAPDLLRVELRQSKL
                     RGQMMIMGTISDPYQPAEHRYRLTRKILQILDRAGNPVQVTTKSTTVTQDLPLLVEMA
                     GRAGAAVNLTITTPNEDLARMLEPRAPAPRQRLAALATLARAGVPVGIFAAPILPGLT
                     DHPDELERLAEAVATAGGKWMMGSALRIGDGFAQPFLEAASRDFPHLLARYQRQARTG
                     FRGSVSRAESEAIHGRLDEIRARYGLASGPPEAPEPRFERQVALPI"
     gene            2547..3683
                     /locus_tag="VFQ54_05425"
     CDS             2547..3683
                     /locus_tag="VFQ54_05425"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004680.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU0164466.1"
                     /translation="MKRRVGVVGAGQLARMMAQAAIPLDIELVLLAADASDGAAKVVP
                     NVIVGSPDDPAALRALADVVDVMTFDHELVEPELLAALEADGVAIWPSAATMALAQNK
                     RRQRAELGAAGFPVPAFANVDSLDDLLAFGAAHRWPVAVKASRGGYDGRGVWIVRDDV
                     QARAVWNEAQEHGTILLVEEFLQLEREFAVIVARNPSGESIVYPVIETVQRNGVCNEL
                     HVPLVADNALLVGAQEIAERIAATIGAVGILAVEVFVADGRVLVNELAPRPHNSGHWS
                     IEGAQTSQFEQHLRAVLDLPLGSTSLMGNAVATINILGPGDGSDPVSRLPGALAVEGA
                     HVHFYGKSARPGRKLGHVTVVGSDAGDTLARAKSAAAILQGEPS"
     gene            3680..4219
                     /gene="purE"
                     /locus_tag="VFQ54_05430"
     CDS             3680..4219
                     /gene="purE"
                     /locus_tag="VFQ54_05430"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU0164467.1"
                     /translation="MTDSTTQRTPRVGVIMGSDSDLPTMQAAIDALAEFGIPYEVRIL
                     SAHRTPDEMFDYARSAHARGLQVIVAGAGGAAHLPGMVASATPLPVIGVPVVATAMAT
                     LGGVDALLSIVQMPAGIPVATVAIGNARNAGLLAARIIGVADPAVREGMIRFQKDLHD
                     RVTAKDESVRIRFEGLQDG"
BASE COUNT          819 a         1354 c         1516 g          814 t
ORIGIN      
        1 cccggtgccg tatccatgac tggagatcgg gctcgacgaa gatgccaaga tgttgctcga
       61 agtgaatgat gctcacttcg tgtgccatgg cgagggttcg cttcgcatcc atgagaccgc
      121 gcacgtagaa gtagaagaac gcgaacgagc cgatgatgat ggtctcgcga atgatcacgc
      181 ggaggtgcga atgatgcgtc gtcgagtcgt agagtctatg gagaaggcga tccatcggtt
      241 tcctgcatcg tgcgccgatt tctggcgaat ccgaagttcg gcctatgata cgtcacagga
      301 gacgccaagg tctctggtta cgggatcgag ccagtcactt tcctgaaatt ggtacactga
      361 cccaacgatc agggtcgtgg cgatgaagcc gggaagagag gacatccgat gagcgatcag
      421 gagcgaagcg agaccgacat cgaagcaccc gatcacgacg ccgccaagga tgtcgacgcg
      481 acgatcgaac gtgaggtcgc cgcagatgaa gccccgatcg cagcgcctcc ggccggagcg
      541 gcggcggaag ccgatcccgc cagcgacgct gacgatctgg atgccgctcc ggacacagcc
      601 gacgatgacg agaccggggt caaacgcgtc agttcgtccg ccgtccagaa tatcgatgcg
      661 catcaggttg tggtggaaag ctcggcggtg cagagcatca aggcgcagcg agtctcgctg
      721 gcgaactcag gcgccaaatc gatcgacgcg cgctcggtca agatggagaa gtccgggacg
      781 gcgatcctga gatcggaacg ggcaacactc aacgactcct cgatcgtgat cgggatgacg
      841 agcgaatttg ccctcaacca gagcagcgcg ggtatcgtga tgtcgggtga gacgaagatt
      901 gctggtgact cgagggtcgg gttcctgacg actggcaccg tcgacgcaac cggcgatatc
      961 cggtcggtgc tgttgacggc aggaacggtc aaggccggtg gcacggtcac gacggtcgtg
     1021 aatcccgtct ccggggccgc gatcggggcg ggcttcgcct ttgtcctgct catcgtccga
     1081 tttgtttttc gcagaggcaa gtagccggtc tcgatcttga aatgtggtgg aaaggtcctg
     1141 gatggaaggt atcaagcgtc tcttcaactt cggcttgggc ggagcgatcg gcactgcggt
     1201 tggcgtcatc gtcggcagct tgctggcgcc gcaatccggc aaggaactgc agcactcgac
     1261 gcagcgcttc gtcgatgagg tcaaggccgg tggcgacgcc gcgcaggcgg aaaccgaggc
     1321 gcagatccgg gcgcgcttcc gcaatcgcgt tggcgatccg actgcgatga cggagacatc
     1381 cgccaaggcc tgatctgcga tcaggcgatt gccaaatgag aacatgtgtt ctatgatcga
     1441 ctcatggaac acgtgcagcc ggcggcgatc gtgccaggct tccaccattc gaatcagacg
     1501 gcttcgacga agcgcggaac gcggtatcac gagacgactg cgaagtcgat cgtgaacgcc
     1561 gtgaagggga tgcgcttcag ctggagcatt tccccgtatc gtgggtgcat tcacgcctgc
     1621 acctattgct atggacgcgc ttcccattcc ttcctcggct acgataccgg agccgatttc
     1681 gaacacgaga tcatcgtgaa gacgaatgcg ccggatctgc tcagggtcga attgcggcag
     1741 tcgaagctcc ggggtcagat gatgatcatg gggacgatct ccgatcccta ccagccagcg
     1801 gagcatcgct accggttgac gcggaaaatc ctccagattc tcgatcgggc cgggaatcca
     1861 gttcaggtca cgacgaagtc gacgacggtg acgcaggatc ttccgctgct cgtcgagatg
     1921 gcgggccggg ccggcgcggc ggtgaacctc accatcacga cgcccaacga ggacctcgcc
     1981 cggatgcttg agccgcgggc gccagcgccg cgccaacgcc tcgcggcgct ggcgacgctt
     2041 gcgcgcgcag gcgttcccgt cggaatcttc gctgcgccga tcctgcccgg tctcaccgat
     2101 catccggacg agctcgagcg gctcgcggaa gcagtcgcga cggcgggcgg gaagtggatg
     2161 atgggttcgg ccctgcggat cggcgatggc ttcgcccagc cgttcctcga agctgccagt
     2221 cgtgatttcc cccatctcct ggcgcgctac cagcgccagg cccgcaccgg cttccgcgga
     2281 tcggtgtcgc gtgccgagtc cgaggcgatt cacggacgcc tcgacgagat ccgcgcgcga
     2341 tacgggctgg catccggtcc tcccgaagct ccggagcccc gcttcgaacg tcaggtggcg
     2401 ctgccaatct gaaccttcat caccatcgca atgcctctcc tcgatacccg gacgaccatt
     2461 cgccggatat cggctatcct gcaagccttg cgaccgcgat ctcgcggccg gatagcgatc
     2521 gtgtgaacag aggatcgatg gcaggcatga agcgacgggt cggagtggtg ggcgccgggc
     2581 aactggcgcg gatgatggcg caggcggcga tcccgctcga tatcgagctc gtcttgctcg
     2641 cggcggacgc cagtgatggt gcggcaaaag tagttcccaa tgtgatcgtg ggatcgccgg
     2701 acgatccagc ggcgctgcgc gcactcgccg atgtcgtcga cgtcatgacg ttcgatcacg
     2761 agctcgtcga gccggaactc ctcgcggcgc tcgaagcgga cggtgttgcg atctggccct
     2821 cggccgcgac gatggcactg gcgcagaaca agcgtcgaca gcgggccgaa ctcggggcag
     2881 ccggattccc cgttccggct ttcgccaatg tcgattccct cgacgatctg ctggcgttcg
     2941 gcgccgcaca tcggtggccg gtcgccgtga aggcatcccg cggcggctac gacggtcgcg
     3001 gcgtctggat cgtgcgggac gacgtccagg cgcgagcggt ctggaacgag gcgcaggagc
     3061 atggcaccat ccttctcgtc gaggaattcc tgcagctgga gcgggagttt gccgtcatcg
     3121 ttgcgcgcaa cccttccggg gagtcgatcg tctacccggt gatcgagacg gtccagcgga
     3181 acggcgtctg caacgagctc cacgttcctc tcgtcgcgga caatgcgctt cttgtcgggg
     3241 cgcaggagat cgcggaacgg atcgccgcga cgatcggcgc cgtcgggatt ctcgccgtcg
     3301 aggtcttcgt tgctgatgga cgggtgttgg tgaacgaact ggcgccccgc ccgcacaatt
     3361 ccgggcattg gtcgatcgag ggcgcacaga cgtcccagtt tgaacagcac ctgcgggcgg
     3421 tgctggacct tcccctcggc tcgacgtcgc tgatggggaa cgcggtcgcg acgatcaaca
     3481 tcctcgggcc tggcgatgga tcggatccgg tctcgcgcct gccgggcgcg ctcgccgtcg
     3541 agggcgccca cgttcatttc tatggcaagt ccgcgcgacc gggacgcaag ctgggtcacg
     3601 tcaccgtggt cgggagtgat gcgggtgaca cactcgctcg cgcgaagtca gccgccgcca
     3661 ttcttcaggg agagccttcg tgaccgattc gacgacgcag agaaccccgc gcgtgggcgt
     3721 gatcatgggg agcgattccg atctcccgac gatgcaggca gccatcgatg ccctggccga
     3781 gttcggaatt ccctatgagg tgcgaatcct ttcggcacat cgcaccccgg acgagatgtt
     3841 cgactatgcg cgctcggcac acgcgcgcgg cctgcaggtg atcgtcgcgg gtgcgggcgg
     3901 cgcggcgcat ctgccgggga tggtcgcttc ggcgacgccg ttgccggtga tcggggttcc
     3961 ggtcgtcgcg accgcgatgg cgacgctggg gggcgtcgac gcactgctgt cgatcgtgca
     4021 gatgccggcg ggaatcccgg tcgccaccgt tgcgatcggg aacgcgcgga atgccggtct
     4081 gctggcggcg cgcatcatcg gggttgccga tcccgcagtc cgcgagggga tgatccggtt
     4141 ccagaaggat ctccacgatc gtgttacggc gaaggacgag tcggtgcgca ttcgattcga
     4201 aggacttcag gacgggtagc acgacgatct gttccggcag tggatagatg agtggccgga
     4261 tcgcgtcatg cacgaagacg catggtcaat cgtcgacggc atgtgcaacg atagtggata
     4321 cattgggggt gggcgaacga cggtggctcc tcgtgtcgac tccccggatc tcctttcaat
     4381 gtgagcccag ggtccaaact cgttcgcgaa cggcatacgt tccaacagag gccccccgtt
     4441 gtccgggagc gatctgcgat tggcgaattt catgactttt cccgaagatg tgtcgggctc
     4501 gac
//
LOCUS       DASTMD010000370         1795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_391987, whole genome shotgun sequence.
ACCESSION   DASTMD010000370 DASTMD010000000
VERSION     DASTMD010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391987"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1795)
                     /gene="cydC"
                     /locus_tag="VFQ54_05435"
     CDS             complement(<1..>1795)
                     /gene="cydC"
                     /locus_tag="VFQ54_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEU0164468.1"
                     /translation="ELSPGELRRVAFGRALARLDGGANLLLLDEPTAHLDASSAETIR
                     RVILGIRARGDVTIVMVAHESHLREIADVVVPVGAGGISILRPMRAATDHHERMPEML
                     PVRAHPDGRADADEITTPGSWRDLVAVLQPWRGKFLLAVLFGVLASLAAISLAGVSGW
                     LIVRASQQPPIMYLLVAIVGVRFFGIGRAVSRYAERLWLHDAVFATMSDLRVRIWRSL
                     AALGPAGGRMLRGERALDRLIGDVDRVRDLAPRVVLPPIVGLITAIATTVALGVLLPA
                     SIGVMAACAVVALVVAPMVALRADRKAKEAEVVIRSRVIRRLASFFAAASDLRVNGLD
                     GRALREIRRLDTWASVQVRRSALASGLGSGIVTLSCVLTSVVMLAVAAPAVKSGEIGV
                     GIVAVLVLTPLALIDPFLGALAAIQQWPALRLGLGRVAVEGLPSAVVADEPVVTMAPI
                     ARLEIDRIAATWPDRARPVFSGLTADLHRGDWLAITGPSGAGKSTLLATLLGFLRPSA
                     GSYRLNGVDARTLSDAAIRIRIAWCPQESHLFDSSLRANLLLARPKDVATTDAEMIAV
                     LNRVGLGGLLASLPAGLETRIGAAGTSLSGGQ"
BASE COUNT          332 a          665 c          585 g          213 t
ORIGIN      
        1 ctggccgccg gagagcgacg tccccgccgc accgatccgc gtctcgagac cggcgggaag
       61 cgacgcgagc aggccaccaa gcccgacgcg gttcaggacc gcgatcatct ccgcatcggt
      121 cgtcgccacg tcctttggcc gcgcgagcag caggttcgcc cgcaacgacg agtcgaagag
      181 atgggattcc tgcgggcacc aggcgatccg gatacggatc gcggcatcgg aaagcgtccg
      241 cgcatccacc ccgttgagcc ggtagctacc ggcgctcggg cggaggaagc caagcaaggt
      301 cgccagcagc gtggatttcc ccgccccgga cggaccggtg atcgccagcc aatcgccacg
      361 atgaagatcg gcggtgagtc cggagaagac cggtcgcgca cgatcgggcc acgtcgccgc
      421 gatccgatcg atctccagcc gcgcgatcgg cgccatcgtc acgaccggct cgtccgcgac
      481 gaccgccgag gggagtccct ccaccgcgac gcgaccgagc ccaagccgca acgccggcca
      541 ctgctggatc gccgcgagag cgccgagaaa cggatcgatc agtgccagcg gtgtcagcac
      601 gagcaccgcg acgatcccga cgccaatctc acccgacttc accgccggtg cggcgacggc
      661 cagcatcacg accgacgtca gcacgcaact gagcgtgacg atcccgctcc cgagtcctga
      721 cgcaagcgca ctccgtcgaa cctggacgct cgcccacgta tcgagacggc ggatctcgcg
      781 gagggcgcgg ccatcaagcc cgttcacccg gagatcggaa gccgccgcga agaacgaggc
      841 gagccgccgg atcacccgcg aacggatcac gacctccgcc tccttcgcct tgcgatcggc
      901 ccggagcgcc accatcggcg cgacgaccag cgccacgacc gcacaggccg ccatgactcc
      961 gatcgaggcc ggcaggagga cgccgagcgc aaccgtcgtc gcgatcgccg tgatcagacc
     1021 gacgatggga ggaagcacca cgcgtggtgc gagatcccgc acgcgatcga cgtccccgat
     1081 caaccgatcg agggctcgct cgccacgcag catccgtccc ccggccggac cgagcgccgc
     1141 cagcgatcgc cagatccgca cgcgcaggtc gctcatcgtc gcgaagacgg cgtcgtggag
     1201 ccagagccgc tcggcgtagc gcgagacggc gcgtccaatt ccgaagaagc gcaccccgac
     1261 gatcgccacc agcaggtaca tgatcggggg ctgctggctc gcgcgcacga tcagccagcc
     1321 ggagactccc gcgagcgaga tcgccgccag cgaggcgagg actccgaaga gcaccgccag
     1381 caggaacttt ccgcgccacg gttggaggac cgcgacgagg tctcgccagg agccgggcgt
     1441 cgtgatctcg tccgcatcgg ccctcccatc gggatgcgcc cgcacgggca gcatctccgg
     1501 cattcgctcg tggtggtccg tcgcggcgcg catcgggcgc aggatcgaga tcccgcccgc
     1561 accaaccggc accacgacgt ccgcgatctc gcgaagatgc gattcgtgcg cgaccatcac
     1621 gatcgtcacg tcgccacgcg cccggattcc gaggatcacg cgacggatcg tctccgccga
     1681 tgacgcgtcg agatgcgccg ttggttcgtc gagcaggaga aggttcgcac cgccgtcgag
     1741 ccgggccagt gcccgtccga aggcgacacg tcgcagttcg cccggactca attcg
//
LOCUS       DASTMD010000371         3823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2354911, whole genome shotgun sequence.
ACCESSION   DASTMD010000371 DASTMD010000000
VERSION     DASTMD010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354911"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VFQ54_05440"
     CDS             <1..1275
                     /locus_tag="VFQ54_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEU0164469.1"
                     /translation="LAERPVWNERLAVGADPSFRYAVDLRTERAWVALFSRHLFVRSD
                     APETAETITVLHAPGLTIDPADYGTRSSTVIALHLGRRVIVIAGTAYAGEVKKAVFTL
                     MQYLLPERGIVTMHCSAAVDSSNRTTLFFGLSGTGKTTLSTDPAFRLVGDDEHGWSEA
                     GIFNLEAGSYAKTLHLSPEREPAIHAAATRAGTVLENVVLDDERTPDFDDDSLAENAR
                     AAYPIDFIPNAVVSGLAPHPSQIVLLTADATGVLPPVSRLTREQAIGIFLLGFTSKVA
                     GTERGVTEPEMTFSPAFGAPFLPLPPERYAELLVARIDAHHPTLWLVNTGWTGGSYAS
                     GASRISLPYTRAIVQAIVDGRLERESFHEEPVFGLTIPVACGDIPPAVLAPIEAWASR
                     SAFEAEARQLKQAFQTGAAEMGIKAEWTRWLD"
     gene            complement(1385..1942)
                     /locus_tag="VFQ54_05445"
     CDS             complement(1385..1942)
                     /locus_tag="VFQ54_05445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEU0164470.1"
                     /translation="MPNPLLLVDLQHGFINEYTHHIPNRVVSLIDRGEYDPILATKFI
                     NIEGGPFHRFIGWHDCTLPPETDLVEEIVPYVAEEMVFSKPGSAGISDELASYLREHE
                     IARITIVGIDTDMCVLKVSLDVFDLGIEPIILTDCCASTAGLQSHLAGLAVLARNIGA
                     DHLRDAGLGGDSLAAPVPNEAATTR"
     gene            2151..3371
                     /locus_tag="VFQ54_05450"
     CDS             2151..3371
                     /locus_tag="VFQ54_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0164471.1"
                     /translation="MAVPRVIIAGAGLGGLCLAQGLRQAGIDVAIHERDAAIDARPQG
                     YRLHIDGRGAVGLHACLPPERYDLVLATSGAPSTQLTVLTTRLRQLKVMRFEGAASDA
                     PEALNTSVDRQVLREILLAGLEDVVHFGHAIVGYDLAGDRVRVRLGDGTTDEGDLLVG
                     ADGVGSTVRRQLVPDAVVAETGEFCIYGRTTLTPEIAGVIPEQLRNGFVAVVGLRGLG
                     MALGMVDFRTPPEEAVRLTPGVALTAREPYVMWALSGEARAFGEGEVDAMTGPELHAR
                     AMRLIGNWHPRLQELVAAAEPDACFPVRIRVAQPVTPWETGPVTLLGDAIHVMSPARG
                     SGANIALKDAGRLRDAIVTAVTGETSLRDAVAGYEREMLQYGFAAVGDSLAVARQGNG
                     PLARILRLLRGNRA"
     regulatory      3672..3771
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT          638 a         1185 c         1312 g          688 t
ORIGIN      
        1 ctcgccgagc gtccggtctg gaacgaacgc ctcgcggtgg gggcggaccc ctccttccgc
       61 tatgcggtcg atctgaggac ggagcgggca tgggtcgccc tgttctcccg tcatctcttc
      121 gtgcgatcgg atgcgccgga gaccgcggag acgatcaccg ttctccacgc ccctggtctc
      181 acgatcgatc ccgcggacta tggcacccga tcgtcgaccg tcatcgcgct tcaccttggt
      241 cgccgggtca tcgtcatcgc tggcactgcc tatgccgggg aagtcaagaa ggccgtcttc
      301 accctcatgc agtatctcct gccggagcgg ggcattgtca cgatgcactg ttcggccgcg
      361 gtggactcgt cgaaccggac gacgctcttc tttgggcttt cgggtacggg caagaccaca
      421 ctgtcgaccg atcccgcgtt ccggctggtc ggtgacgacg agcatgggtg gtcggaggca
      481 ggcatcttca acctggaggc ggggtcctac gcgaagaccc tccacctctc cccggaacgc
      541 gaacccgcga tccatgccgc ggcaacgcgt gcgggcaccg tgcttgagaa tgtggtgctc
      601 gatgatgaga gaacgccgga tttcgacgac gattccctcg ccgagaatgc ccgtgccgcc
      661 tacccgatcg acttcattcc gaatgccgtc gtctccgggc tcgctcccca tccgtcgcag
      721 atcgttctcc tcacggcgga tgcgaccggc gtccttcctc ccgtttcccg cttgacgcgg
      781 gagcaggcga tcggcatctt cctgctcgga ttcacctcga aggtggcggg cacggagcgc
      841 ggggtgacgg agccggagat gaccttctcc ccggcgttcg gcgcgccatt cctgcccctc
      901 ccgcccgaac gctatgccga attgcttgtc gcacggatcg acgctcacca tccgacgctc
      961 tggctggtca acactggctg gaccggcggg tcatacgcgt ccggagcgtc gcgtatcagc
     1021 ctgccgtaca cgcgggcgat cgtgcaggcg atcgtcgatg gtcgactcga acgcgagtct
     1081 ttccatgagg aacccgtctt tggcctgacg attcccgtgg cctgtggaga catccctccg
     1141 gcggtgcttg ccccgatcga ggcctgggct tcgcggtccg cgttcgaggc ggaagcacgt
     1201 cagctcaagc aggcatttca gaccggggcg gcggagatgg ggatcaaggc ggagtggacg
     1261 cgctggctcg actgatcttg atgtgacata aggcgaggca aggtcggcat tccaaggatg
     1321 ccgcatctca tggaacaggg gtcatgccgg ggatcctcat agcgggacga ggggtacgaa
     1381 ccggtcaccg ggtagtcgct gcttcgtttg gaacgggcgc ggcgagggag tcgccgccga
     1441 ggccggcgtc acggaggtga tcggcgccga tgttgcgcgc gagcacggcc agcccggcga
     1501 gatgcgactg cagaccggcg gtgctcgcgc agcagtcggt gaggatgatc ggctcgatcc
     1561 cgagatcgaa gacatcgagc gagaccttca gcacgcacat gtcggtgtcg atgccgacga
     1621 tcgtgatccg ggcgatctcg tgctcgcgca ggtagctcgc cagttcatcg gagatgccgg
     1681 cgcttcccgg cttcgagaag accatctcct ccgccacgta tggcacgatc tcctcgacga
     1741 gatcggtttc cggtggcagc gtgcagtcgt gccagccgat gaacctgtgg aacggtccgc
     1801 cttcgatgtt gatgaacttc gtcgccagga tcggatcgta ctcgccacga tcgatcagcg
     1861 aaacgacccg gttcggaatg tggtgcgtgt actcgttgat gaacccgtgc tggagatcga
     1921 cgagaaggag cgggttgggc atctggttgg gggaactcct gcaagtgccg ggatgccgag
     1981 ggtggctttg cccgccgtgc gatcctgata ccggacgata cgtatccggg gtatgacgca
     2041 ggggacgtgc cgccgtgatg gaacttggga atcgaggcgt gagaaacacc gggtcggtgc
     2101 tggcacggct cgatggcggg atgtgattcg tgcctgaagg agggtagatc gtggcagttc
     2161 cgagagtgat catcgcggga gcgggactgg gcggactctg cctcgcacaa ggactgcgac
     2221 aggcggggat cgatgtcgcg atccacgagc gggacgcggc gatcgatgcg cgtccccaag
     2281 gctaccgcct ccacatcgac ggtcgcggcg cggtggggct gcatgcctgc ctgccaccgg
     2341 agcggtacga tctcgtcctt gcgacatccg gggcgccgag cacccagctt accgtcctca
     2401 cgacccggct ccgccagctc aaggtgatgc gattcgaggg tgccgcgagc gacgcgcccg
     2461 aggcgttgaa cacctccgtc gaccggcagg tcctccgcga gatcctgctc gcggggctcg
     2521 aggatgtcgt gcacttcggg cacgcgattg tcggctacga cctcgccggg gatcgcgtcc
     2581 gcgtccgtct cggggatggg acgaccgacg agggcgatct tctcgtcggg gcggacggcg
     2641 ttggctcgac ggtccgccgg cagctggttc cggacgccgt ggttgcggaa accggcgagt
     2701 tctgcatcta cggccggacg acattgacgc cggaaatcgc cggcgtgatt ccggagcagc
     2761 tgcgaaacgg attcgtcgct gtcgtcgggc ttcgcgggct cgggatggca ctggggatgg
     2821 tcgatttccg gacgccgccg gaggaagccg tgcggctcac ccccggcgtg gccctcaccg
     2881 cccgcgagcc atacgtgatg tgggcgcttt cgggtgaagc gcgggcgttc ggcgagggcg
     2941 aagtcgacgc gatgaccggg ccggagctgc acgcgcgcgc catgaggctg atcgggaatt
     3001 ggcatccgcg ccttcaggag ttggtcgcag cggcggagcc cgacgcctgc ttcccggtcc
     3061 ggattcgcgt cgcacagccg gtcacgccat gggagacggg tcccgtcacg ttgcttggtg
     3121 atgcgatcca tgtgatgtcg ccggcgcggg gatcgggcgc gaacatcgcg ctgaaggacg
     3181 ccggtcgact ccgcgacgcg atcgtcaccg ccgtcaccgg cgagacctcc ttgcgggatg
     3241 ccgtcgccgg atacgagcgg gagatgctgc agtacggctt cgccgcggtc ggcgacagtc
     3301 tcgctgtggc gcgccagggg aacggtccgc tcgcgcgaat cctccgcctc cttcgcggaa
     3361 accgggcgtg agcgtcgggg aggcgtcgtg ccggcgtcgg ggaggcaatc acgtggggcg
     3421 ccgggacgga tatccacccc tggcgatgag tgtccggtgt gcgatacgat gatacgtatg
     3481 tcagaatagc tgttgaatgc gagtcgattc gctcggatcg tttccgccat gcctgacaat
     3541 ccagaggggc tgttggcgct ggaccaagcc gccgaagagc cgaatggcaa tactatcaaa
     3601 gcggaagaag ctggcattat ctattgacac ttcatactgg ataccagtag agtcgtccca
     3661 caactgaata gacctcatcc agaggggcag agggaccggc cctatgaagc cccagcaacc
     3721 acccagcaga ggatggtgct aattccggca ggcaatcctg gaagatgagg gcacgcacac
     3781 acaggcagtc catgccttgt ctgcaattgg ccgccctcat cat
//
LOCUS       DASTMD010000372         1937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2445280, whole genome shotgun sequence.
ACCESSION   DASTMD010000372 DASTMD010000000
VERSION     DASTMD010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2445280"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFQ54_05455"
     CDS             complement(<1..714)
                     /locus_tag="VFQ54_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164472.1"
                     /translation="MSTKTPRPVDPRSLEGIRDRFVNATSATIIHTTGGDFRISNLPL
                     LREVDGGLEQVVRVRIPAGTPGTTVSARLLSGGSIVAEGSGAIMGTPESLLLFVPEVD
                     DACLLDIEIRLDDVLLGSAQHEVTPQRKFTVHIVHHSHYDIGYTDPQSTVLEAQLNFI
                     DYALELATLTDDWPSDAQFRWNIEVNWPLKHWLRTRPKHAREALLRRVKEGRFEVHAL
                     PFSMHTEAYAIDELAQQLAF"
     gene            complement(742..>1937)
                     /locus_tag="VFQ54_05460"
     CDS             complement(742..>1937)
                     /locus_tag="VFQ54_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0164473.1"
                     /translation="RHSNGAHWGRFVRTIVEVESDDGRIGLGEMGGGGESAEAAIAGL
                     KYYLLGHDVFHLETLRFKIANPTASLYNNRTQLLAAIEFACLDLIGQKLDQPVYNLLG
                     GKLRDEVPFASYLFFRYANPDTGEGEIRTPEQLAAHAVDLKERFGFTSHKLKGGVFPP
                     DYELEAYHALAAALPKDRLRFDPNGAMSVEEAIRFGHAIEGLRNDYFEDPTWGLNGMR
                     RVRSMVRIPTATNTVVVNFEQLAANILDLAVDVILLDTTFWGGIRSCIKAAGVCETFQ
                     LGVAVHSSGELGIQLATMLQLGATLNNLTYAADAHYHHLTDDIIVGGKMAYANGAIRV
                     PDGPGLGVTLDREKLAEYAELYEELGGYPYDRDPGRRDWFALVPADRNTWADPTDSTT
                     PSLKP"
BASE COUNT          320 a          560 c          679 g          378 t
ORIGIN      
        1 gaaggcgagc tgctgcgcga gctcgtcgat cgcataggcc tcggtgtgca tgctgaaggg
       61 gagggcatgg acctcgaatc ggccctcctt gacgcggcga agcagtgcct cgcgagcgtg
      121 cttcggccgc gttcgcagcc agtgcttgag tggccagttg acctcgatgt tccagcgaaa
      181 ctgcgcgtcc gacggccagt cgtcggtgag cgtcgcgagt tcgagcgcgt agtcgatgaa
      241 gttgagctgc gcttcgagca cggtcgactg cgggtcggtg taaccgatgt cgtagtgcga
      301 atggtggacg atatggacgg tgaattttcg ctgcggtgtc acctcatgct gcgccgatcc
      361 cagcaggaca tcatccaggc gaatctcgat atcgagaaga caggcgtcat cgacttccgg
      421 gacgaagagg agcagcgatt ccggtgtccc catgatcgcg ccggatcctt cggcgacgat
      481 ggagccaccg ctgagaagtc tggccgagac ggtggttccg ggcgtcccgg ccgggatgcg
      541 aacgcggacg acctgctcga gcccgccgtc gacctcgcgc aggagcggca ggttgctgat
      601 ccggaaatcg cctccggtcg tgtgaatgat tgttgccgat gtcgcattga cgaagcgatc
      661 gcgaatgccc tcgaggctgc gcggatcgac gggacgtggg gtcttcgttg acacgggatc
      721 gtctccttct tctggtgttt gttacggctt gagtgacggt gtggtgctgt cggttgggtc
      781 cgcccaggtg ttgcggtcgg ccgggacgag ggcgaaccag tcgcggcgcc cgggatcgcg
      841 atcgtacgga tagccgccga gttcctcgta aagctccgcg tattcggcaa gtttttcgcg
      901 gtcgagcgta acgccgagcc ccgggccgtc cgggacccgg atcgcgccgt tcgcgtatgc
      961 catcttgccg ccaacgatga tgtcgtccgt gaggtgatgg tagtgggcgt ccgccgcgta
     1021 ggtgagattg ttgagcgtcg cgccgagctg gagcatcgtc gcgagctgaa tcccgagttc
     1081 gccggacgaa tggaccgcga cgccgagctg gaacgtctcg cagacgcccg ccgccttgat
     1141 gcatgaccgg attccgcccc agaaggtggt atcgaggagg atcacgtcga ccgcgagatc
     1201 gaggatgttc gccgcgagtt gctcgaagtt gacgaccacc gtgttggtcg cggtcgggat
     1261 acgcaccatc gagcggacgc gtcgcatgcc gttcagaccc caggtcgggt cctcgaagta
     1321 gtcgttacgg agtccctcga tcgcgtgccc gaagcgaatc gcctcctcca cgctcatcgc
     1381 gccgttggga tcgaagcgca accgatcctt cggaagcgcg gcggcgagcg cgtggtaggc
     1441 ctcgagttcg tagtccggcg ggaagacgcc gcccttcagc ttgtgactgg tgaagccgaa
     1501 gcgttccttc aagtcgaccg catgcgccgc gagttgttcc ggggttcgaa tctctccttc
     1561 gccagtgtcg ggattggcgt agcggaagaa gaggtaactg gcgaagggaa cctcgtcgcg
     1621 cagcttgccg ccgagcaggt tgtagaccgg ctggtcgagt ttctggccga tcaggtcgag
     1681 gcaggcgaat tcgattgcgg cgaggagctg ggtgcggttg ttgtagaggc tggcggtcgg
     1741 gttcgcgatc ttgaagcgaa gggtttccag gtgaaagacg tcgtgcccga ggaggtaata
     1801 cttcagcccg gcgatcgcgg cctccgcgct ttcgccgccg cctcccatct cgccgagacc
     1861 gatgcgtccg tcgtcggact cgacctcgac gatggtgcgt acgaagcgtc cccagtgcgc
     1921 gccattcgag tggcgaa
//
LOCUS       DASTMD010000373         3066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1106884, whole genome shotgun sequence.
ACCESSION   DASTMD010000373 DASTMD010000000
VERSION     DASTMD010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106884"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VFQ54_05465"
     CDS             complement(<1..608)
                     /locus_tag="VFQ54_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164474.1"
                     /translation="MFRWIATCALVGLILGGTFIPASAGSARPASSGDVAAAAITGIT
                     ISTSDGEDLPAGTEIQIIKVDPEPSEPFSDQTLATAQSSPYVISGLDMDPDALYSVNV
                     FAPSDYAPFASGTFHGGASQSFQIDAIPTSHVTITTVDGGDIPAGTEIQVIKVDPEPS
                     ESFSDQTLGTSIPSGYDLVLANVDPGALYSVNVFAPVTYLPYA"
     gene            complement(985..1257)
                     /locus_tag="VFQ54_05470"
     CDS             complement(985..1257)
                     /locus_tag="VFQ54_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164475.1"
                     /translation="MTPPRRGIARFPPRDRPPSPEGLATTEQRVVFPGDSETGVKDPV
                     VRLDGTRWHACCHLLDVPGAEDLMEMAHASSDDGITWSPPERSLRG"
     gene            1348..2706
                     /gene="melA"
                     /locus_tag="VFQ54_05475"
     CDS             1348..2706
                     /gene="melA"
                     /locus_tag="VFQ54_05475"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF011657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU0164476.1"
                     /translation="MATAAAESQAPASDAQKTGPKIVMIGAGGVVFPLRLMGDMLTFP
                     SLRGATYALVDLDRSRAERNAVSARDLAKHYGFPAAIEATDDQREALTGADYVIVTFM
                     VGGLDVYRHDMEIPRKYGVDQRIGDTLGPGGVFRFLRSAPAYASIAKDMLELCPNALL
                     INYANPMAMNCWYLTELGIAAVGLCHSVQGTSRMLATQIGEQIEEVTFQTAGINHQAW
                     FLKFQKGHEDLYPRLRETMIAKNLGGKNADGSIPVEKVRTEIMQTFGYFHTESSPHAS
                     EYLPYFRKNDALIDEYVTARWDYDEIAARVPAREADVSVIEALKKELEPSHEFGAWII
                     NAMETDVPTVIYGNVPNRGLIDNLPEGSCVEVACLVDRNGIQPTRYGALPPQCAALNR
                     TNIGVQELAVQAVLTGDKEHIYHAVALDPLTAAVCTLDEIRAMTDELFAANASYLPAH
                     WR"
BASE COUNT          556 a          952 c         1008 g          550 t
ORIGIN      
        1 gcatagggca ggtaggtcac cggagcaaag acattgaccg aatagagcgc gccgggatcg
       61 acattcgcaa ggacgagatc gtagccgctg gggatggatg tccccagcgt ttgatcggag
      121 aagctctcgc tcggctccgg gtcgaccttg attacctgga tctccgtccc cgcggggata
      181 tcgccgccgt cgacggtcgt gatcgtgacg tggcttgtcg ggatcgcatc gatctggaat
      241 gactggctgg cgccgccgtg gaaggtgccc gaggcaaacg gggcatagtc gctgggcgca
      301 aagacgttca ctgaatagag cgcgtccgga tccatgtcga gcccggagat gacgtatgga
      361 gaagactggg ccgtcgccag cgtctggtca gagaagggct cgctgggctc gggatcgacc
      421 ttgatgatct ggatctccgt tcccgccggc agatcctcgc cgtccgaggt cgagatcgtg
      481 atgccggtga tcgcggcggc ggcaacgtcg ccgctggaag ccggcctcgc gctgccggcg
      541 gaggcaggaa tgaaggtgcc gccgaggatg agcccgacga gtgcgcacgt ggcaatccaa
      601 cgaaacacgg agttcatcat tctccccttg gggtcgaatc gggcgccgtg cccgggccat
      661 gacgatccat gtcgcgtccc cgacggcgtg ccaatgtgcc ggatgtggtt ctgggtccct
      721 ttccactgtt tgtagcacgt ggcacggctc ccggccaaac gccgcctgcc gcgacacacc
      781 cgaatgtgtc gcgcgcagcg tcttgccggt tcaacgcgag cgtgtgtggt gaatcgctgg
      841 cgcatcttta tcgtgttcgg ggtgagggtc gtcggccgcg gggaccgcga gcccggtccg
      901 ttcgaaccag ttctcctcct tcgtggcgcg accatcggga aggaccgcgg tcacacgcgc
      961 gccgcgcaca tcccacacct ccagtcagcc ccggaggctg cgctcgggtg gcgaccaggt
     1021 gatgccgtcg tcgctcgacg catgagccat ctccatcaga tcttccgcgc cgggaacgtc
     1081 gagcagatgg caacaggcgt gccaacgcgt gccgtcgagc cggacgaccg ggtccttgac
     1141 gccggtctcg ctgtcaccgg gaaagacgac gcgttgctcg gtcgtggcaa gcccttccgg
     1201 ggaaggcgga cgatcgcggg gagggaagcg ggcgatgccg cgcctcgggg gcgtcacgtc
     1261 gcgggggttg cgtgacccgg tgacgatgcg aagataaggt ggtcaccctg cctgaccgtt
     1321 cgacgcttga tccacgaagg acccttcatg gcaactgctg ctgctgagtc acaggctccc
     1381 gcttccgatg cccagaaaac cggaccgaag atcgtgatga tcggcgccgg tggcgtcgtc
     1441 tttccccttc gtttgatggg ggacatgctc accttcccgt cgcttcgcgg tgcgacctat
     1501 gcgctggtgg atctcgaccg cagccgcgcc gagcgcaacg cggtttcggc gcgcgacctc
     1561 gcgaagcact acggcttccc ggcggcgatc gaggcgaccg acgatcagcg cgaggcgctg
     1621 accggcgccg actacgtgat cgtcaccttc atggtcggcg gtctcgatgt ctaccggcac
     1681 gacatggaga ttccgcgcaa gtacggcgtc gatcagcgga tcggcgacac gctcggcccg
     1741 ggcggcgtct tccgcttcct gcgctcggct ccggcgtatg cgagcatcgc gaaggacatg
     1801 ctggaactct gcccgaacgc attgctcatc aactacgcga acccgatggc gatgaactgc
     1861 tggtacctga cggagctcgg gatcgccgcg gtcgggctct gccacagcgt gcaggggaca
     1921 tcgcggatgc tggcaacgca gatcggcgag cagatcgagg aggtgacctt ccagacggcg
     1981 ggaatcaatc accaggcgtg gttcctcaag ttccagaagg ggcacgagga cctctatccg
     2041 cgcctccggg agacgatgat cgcgaagaac ctcggcggca agaacgcgga tggctcgatt
     2101 cccgtcgaga aggtccgcac ggagatcatg cagacgttcg gctacttcca cacggaatcc
     2161 agcccgcacg ccagcgagta cctgccctac ttccgcaaga acgacgcgtt gatcgacgag
     2221 tacgtgaccg cccgctggga ttacgacgag atcgcggcgc gcgtgccggc ccgggaggcc
     2281 gatgtctcgg tgatcgaggc gctgaagaag gaactcgagc cgtcccatga gttcggcgcc
     2341 tggatcatca acgcgatgga aaccgacgtg ccgaccgtga tctacggcaa cgtgccgaac
     2401 cggggactga tcgacaacct tccggaagga agctgtgttg aggtcgcctg tcttgtcgac
     2461 aggaacggca tccagccgac gcgctacggc gcccttccgc cgcagtgtgc tgccctgaac
     2521 cggacgaaca tcggtgtgca ggaactcgcc gtgcaagccg tcctcaccgg cgacaaggag
     2581 cacatctatc acgcggttgc gctcgatccg ctgacggcgg ctgtctgcac cctggacgag
     2641 atccgggcga tgaccgacga actcttcgcc gccaatgcga gctatctccc cgcccactgg
     2701 aggtagcgtt ccccgttccg atcgacctgg atgaagaaga ccacggcagg tattgccgtg
     2761 gtcttctcgt gtgcgtcgct tctcgcattc ctgccagaga catcgattga cgccaatagg
     2821 gcgaatgtct cgctggccat agggccgctt tggtgtcgat taatcccgat ttcaccccgc
     2881 atcgattgaa atcatgtccc gatcgtagac aatgattgca tcgatacgtg cagtcacact
     2941 ggcccgtacg cgcaaaggag tcgaaagatg tcgctcgatc ttcaacggcg gatgcttgcg
     3001 gaagcgatcg gaacgttctt gctcgtcttc attgggtgtg gtgcggtggt gacgaacacg
     3061 gtcaac
//
LOCUS       DASTMD010000374         4016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_658912, whole genome shotgun sequence.
ACCESSION   DASTMD010000374 DASTMD010000000
VERSION     DASTMD010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658912"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VFQ54_05480"
     CDS             complement(<1..753)
                     /locus_tag="VFQ54_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0164477.1"
                     /translation="MTGFLPNPVNALMRDAVIGTSSRRDLIRRGSALGLSATLMGTLL
                     RAQSIGAQATPSASADLNKPVGWSIKAPAGLRTDLKGKKITVMLGAAGPGESFDQATC
                     DAFTAATGISVTYLRGADSSTDRLQFYLQSFGAETTDVDACQIDVIWPGTLAAYAVDL
                     TDVYTSQGVSSFQRIVENNTVNGMLVGIPWYTDAGLLYYRTDLLEKYGFSAPPQTWQE
                     LTDQATTIRNGEKEGNASFTGFTWQGAAYEGLV"
     gene            complement(1093..1530)
                     /locus_tag="VFQ54_05485"
     CDS             complement(1093..1530)
                     /locus_tag="VFQ54_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0164478.1"
                     /translation="MGKIFELAPEGIERLLHKALIGRIACAAEGEGRPYLVPIGFGYD
                     GEAVYFLSGPGRKIDIMRKQPLAAFEVDEGEAEDRWRSVVADGTYEELASVEQRRNAM
                     KVLFGEGPYPEVGDHMIFFRLVLTRKSGRYEVPDDEAEQIGAS"
     gene            complement(1632..2780)
                     /locus_tag="VFQ54_05490"
     CDS             complement(1632..2780)
                     /locus_tag="VFQ54_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164479.1"
                     /translation="MSEGPKPFSTDWYRDPAAPERIARRLPPRPARILIGVASKLPNE
                     RALSILVVLFLVISSFWFAGPRGDAHDGNGTGVGTPLPTIAFSAPEPTAAPDISTDSL
                     QVAEAPEKTAPKLDLQAPSDGETTNGLIPKNRIITYYGFPGNDSMGVLGEQNMQDSLT
                     ALKQQAKAYEAADPTRPVLVGFEVIASVGQRDPQADGSYLLDTPASVLDEYADFCEKN
                     GILLFLDVQIGRRTVSDEVQGLEPWLKKPFVHLAIDPEFAVKEGQVPGVDIGSVDASD
                     ITWTQNWLVKMAKDNDLPPKILMVHQFHDGMIQNKDKLAPVTGVQLVIDGDGWGPPDS
                     KRSMYEFVNKEQEIQYNGIKIFYKQDDPILTPEEVVKMDPVPDVVVYQ"
     gene            3425..3928
                     /locus_tag="VFQ54_05495"
     CDS             3425..3928
                     /locus_tag="VFQ54_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164480.1"
                     /translation="MASLEKRHVSRRALVASSLVGIGLAAGGRTAFADPIDIPTVALP
                     ASAATPAPKNPLYTGPIADLWEQPWLLEGKSISIKGTILQRLVAPAGRGYRISDDGII
                     YRSLLDLDVPSESEVFVVTNDDLTSIADLRTVEAIGSYGGIADDDVNGHHGIPIVIAA
                     VIRRATS"
BASE COUNT          732 a         1291 c         1222 g          771 t
ORIGIN      
        1 gacgagtccc tcatacgcgg ctccctgcca cgtgaagccg gtgaaggaag cgttgccctc
       61 cttctcgcca ttgcggatgg tcgtcgcctg atcagtcagt tcctgccatg tctgcggagg
      121 cgcgctgaat ccgtacttct cgaggaggtc ggtccggtag tagagcaagc cggcatccgt
      181 gtaccacgga atcccgacaa gcatgccgtt caccgtgttg ttctcgacga tgcgctggaa
      241 actggacacg ccctgcgacg tatagacatc ggtaaggtca accgcgtacg cggccagcgt
      301 tccgggccag atcacatcga tctggcaggc atcgacatcc gtggtttcgg caccgaacga
      361 ctgcaggtag aactggagcc gatccgtcga ggaatcggcg ccgcgcaggt aggtcaccga
      421 aatcccggtc gcagccgtga aggcgtcgca ggtcgcctga tcgaaggact cccccggccc
      481 ggcggctccg agcatgaccg tgatcttctt gcccttcaga tcggtacgaa gaccggcggg
      541 tgccttgatc gaccagccaa ccggcttgtt gagatcggcc gatgccgatg gcgtggcctg
      601 cgcaccgatc gattgggcgc gcaggagcgt gcccatcagg gtcgcactga gtcccagggc
      661 ggatcctcga cggatgagat cccgacggga actggtgcca atgaccgcgt cacgcatgag
      721 ggcgttcaca gggttcggga gaaaaccggt catcgtgccc cactccttgc cttggtgctg
      781 acgatgccac cggtcgggat gcacaaggca ttcacaacgt ccatacgcgc cgcgcaccgt
      841 gcacataaac cgacgaccgg cgacttcggg ctatcaaact cgtcttgcta ccgggtttga
      901 tacatccgaa cacgcctggc cgtcaaggat ttcccgacag cttctctcgc tgcaggcatg
      961 gcaatgggcg tcgatgcggg ggaattcccg tcatccgccg ctggggaggg cctcacaggg
     1021 gatgccccaa acggaggagg gcgagcctgg atcggctcgc cctcctccgt ctgtgatctg
     1081 gccaggggtc gttcaggacg ccccgatctg ctccgcctcg tcgtcgggca cctcgtatcg
     1141 ccccgacttc cgggtgagga cgagccggaa gaagatcatg tgatccccga cttccggata
     1201 tggcccctcg ccgaacagca ctttcatcgc gtttcgtcgc tgctcgaccg aagcgagttc
     1261 ctcgtaggtg ccatcggcga cgacgctccg ccagcgatct tcggcttctc cctcgtcgac
     1321 ctcgaatgcg gcgagcggct gcttgcgcat gatgtcgatc ttgcgaccgg ggccgctcag
     1381 gaaatagacc gcctcgccgt catagccgaa gccgatcgga acgaggtagg ggcgtccttc
     1441 gccctccgcc gcgcaggcga ttctcccgat cagcgccttg tgtagcagac gctcgatccc
     1501 ctcgggagcg agttcgaaaa tcttccccat gagccctctt cgattcacgc gtctggtctg
     1561 tccgggtacc ggaaccggcg gtcgcacgcc cacgacagag gtgcgatcgc gatccgatga
     1621 cccgttacga cttactggta tacgacaaca tccgggaccg gatccatctt gaccacttct
     1681 tccggcgtca ggatcggatc gtcctgcttg tagaagatct tgatcccgtt gtactggatt
     1741 tcctgctcct tgttcacgaa ctcgtacatg cttcgcttgc tgtcgggtgg cccccagccg
     1801 tccccgtcga tcacgagctg cacgccggta accggtgcga gtttgtcctt gttctggatc
     1861 atcccatcgt ggaactgatg gaccatcagg atcttcggcg ggaggtcgtt gtccttggcc
     1921 atcttcacga gccagttctg cgtccatgtg atgtcggagg cgtcaaccga tccgatgtcg
     1981 acgccgggga cctgtccctc cttcacggcg aactcgggat cgatcgcgag gtggacgaaa
     2041 ggcttcttca gccacggctc gagcccctga acctcgtcgg agacggtgcg tcgcccgatc
     2101 tgcacgtcga ggaagagcag gatcccgttc ttctcgcaga agtcggcgta ctcatcgagt
     2161 acggaggccg gagtgtcgag cagatagctg ccatcggcct gcggatcccg ctgaccgacc
     2221 gaggcaatga cctcgaagcc gaccagcaca ggacgtgtcg gatccgccgc ctcgtacgcc
     2281 ttggcctgct gcttgagcgc ggtcagcgaa tcctgcatgt tctgctcgcc gaggacgccc
     2341 atgctgtcgt tgccggggaa tccgtagtac gtaatgatcc tgttcttcgg gatgagtcca
     2401 ttcgtcgtct cgccatcaga cggagcctgg agatccagtt tcggcgccgt cttctccggc
     2461 gcttcggcga cctggaggct gtcggtcgaa atatccggcg cagcggtcgg ctcgggcgcg
     2521 gagaaggcaa tcgtcggcaa gggagtaccc accccggtgc cgttcccatc gtgtgcatcg
     2581 ccgcgcggtc cagcgaacca gaacgacgag atcacgagga aaagcacgac caggatactc
     2641 agggcacgct cgttcgggag tttgcttgcg actccaatca ggatccgggc cggacgcggc
     2701 ggcaaccggc gcgcgatgcg ctctggcgcc gcgggatcgc ggtaccagtc cgtggagaag
     2761 ggctttggtc cttcactcat aaaagctcga ttctcgggcg tcaacacgcg atgggcgacg
     2821 actttcggcg atccggccca gacatctttc ctgccggtct gcggtcgaac atgacgtcag
     2881 atcacgtccg gccgccttcg catcacaccc atctcgacgg gttcgacgac gcacgacata
     2941 cgcatcgatc ttgcggggaa cgatgtgtcg tacctcgcac tatgccacga tccagaccga
     3001 aactccacct tttacttagg taatgcacga ttcccgaaac acgacatata tgttaacgac
     3061 gacaacacca tgctcctgct tcgtccactt cacgaacgga aatcaccctc gcacggacac
     3121 actattcacg gtcacgccag ccgtcccgcg atcgccaaat cgaaaatcag ctcctggcgg
     3181 agcgcggatt cccggtcgct gtgatctcgt cggaaaccat gctccggttc gcgtcaccgg
     3241 gcgatcactg ccgcgggtga acgacccgca gccccgcgcc gtcatcccga tcgccgaacg
     3301 ccacgggatc ggcgcaaccc tgaatgcctc cacacggttg tgtctggcgc gctcccatcg
     3361 gagaatggat cctgggtcac aacgttggtt gcattcacat cagactgcat gcggggaggc
     3421 gaggatggca tcgctggaga aacgtcatgt ctcacgacgc gcgctcgtcg cgtcatcgtt
     3481 ggtcgggatc ggtctcgccg cgggcggcag aaccgcattc gccgacccga tcgacattcc
     3541 gaccgtcgcg cttccggctt ctgcggcaac gccggcgcca aagaacccgc tctacaccgg
     3601 acccatcgcc gacctctggg agcaaccgtg gctcctcgag gggaaatcga tctcgatcaa
     3661 ggggacgatc ctgcaacgtc tcgtcgctcc cgctggtcgc ggctaccgga tcagcgacga
     3721 cggcattatc taccggtcgc ttctcgatct cgatgtgccg agtgaatccg aggtcttcgt
     3781 ggtcaccaac gacgacctga cgtcgatcgc ggatctccgc acggtcgagg cgatcgggag
     3841 ctacgggggc atcgccgacg atgacgtgaa cggtcatcac ggaatcccca tcgtgatcgc
     3901 ggcggtgatc cgcagggcga catcgtagat atcacctgtc gttgaagagc ggagtggtga
     3961 cgaaggggga ggggtggccg ccggtcgcag gcggtgtgcg gctggttgcg gcgatg
//
LOCUS       DASTMD010000375         1680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1909649, whole genome shotgun sequence.
ACCESSION   DASTMD010000375 DASTMD010000000
VERSION     DASTMD010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909649"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1680)
                     /locus_tag="VFQ54_05500"
     CDS             complement(<1..>1680)
                     /locus_tag="VFQ54_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HEU0164481.1"
                     /translation="SPTKEPHSEPSLIDPDLHIGFSLEQGVPSLGVTASYEALSGLGR
                     AEYLAWLAGGRKDPHIPREFIRIFLCGIERRLLIDIARDDSLRSELDSIRDAVVDLAM
                     TYQRMMYPGFTDEIADALEILGLDPVQFASDPASAPDIDMMPWIPPDILPLGLGCFTA
                     QRTPIPADWAFTWAWYRPEVPTRTPMHRAPQEYQELFIKLYREQFKQGMVLRRGKKTA
                     RVTATATNQSIGYVTLDLSHIPDVFARQKPIEDLRLLAIRASSKLDGYSRWIQKNPYD
                     ADTLAAIAQYPPELISQELPAAQEFFLWITERMDDRSYVELTGADLFEVWSVTAPQRD
                     RLTPKEAQSLGYVFGWFGFGIEPDFRLGKPALDATMPVIVFRRDSVEGERRYAPIPTM
                     SSESFHIASLATHLTLALIAVEQVVTPDVIPLVIAELDGLFEMDALEKARLHAYLLLQ
                     IEKPLKVTGHKSRIVGLSEHQREVVAAFVISVAARHGSISPQIVKLVGQLYAQLDLDR
                     TRVSSDLHGAMTSSSPSPVRRPGGSAIRTEVRLDRGIIARKEREASEVSTL"
BASE COUNT          294 a          509 c          583 g          294 t
ORIGIN      
        1 ggagggtgga gacctcggat gcctcccgct ccttgcgggc gatgatcccg cggtcgagcc
       61 ggacttcggt ccggatcgcg gatccacctg ggcgccgaac cggcgacggg ctggacgacg
      121 tcatcgcccc gtgaagatcg ctgctgaccc gcgtcctgtc gaggtcgagt tgcgcgtaga
      181 gctgcccgac gagcttcacg atctgcggcg agatgctgcc atggcgggcg gcgacggaga
      241 tcacgaatgc cgcgacgact tcgcgctgat gctcgctcag cccgacgatc cggctcttgt
      301 gaccggtgac cttgagcggc ttttcgatct ggaggaggag ataggcgtgg aggcgcgcct
      361 tctcgagggc atccatctcg aagagcccgt cgagttcggc gatcacgagc ggaatcacgt
      421 ccggtgtcac gacctgctcg acggcgatca gcgcgagcgt gaggtgcgtg gcgagactgg
      481 cgatgtggaa cgactccgaa ctcatcgtcg ggatcggggc gtaacggcgc tcgccttcga
      541 cgctgtcgcg gcgaaagacg atgaccggca tcgtcgcgtc gagcgcgggc ttgccgaggc
      601 ggaaatcggg ttcgatcccg aagccgaacc agccgaagac gtacccgagc gactgcgcct
      661 ccttcggcgt caaccggtcc ctttggggcg ccgtcaccga ccagacctcg aagagatcgg
      721 ccccggtgag ttcgacgtag gaacgatcgt ccatccgctc ggtgatccag aggaagaatt
      781 cctgcgcggc cggcagttcc tgcgagatca gttccggtgg gtactgggcg atcgcggcca
      841 gcgtatcggc atcgtaggga ttcttctgga tccagcgact gtagccgtcg agcttgctcg
      901 acgcgcgaat cgcgagcagg cggagatcct cgatcggctt ctggcgcgcg aagacatccg
      961 ggatgtggga gagatcgagc gtcacgtagc cgatgctctg attcgtcgcc gtcgcggtga
     1021 ccctcgcggt cttcttcccc cgccggagca ccatcccctg cttgaattgc tcgcgataga
     1081 gcttgatgaa gagctcctgg tactcctgcg gcgcgcgatg catgggagtg cgcgtcggca
     1141 cttccggacg gtaccaggcc caggtgaagg cccaatccgc cgggatcggg gtgcgctggg
     1201 cggtgaagca gccgagaccg agcggcagga tgtcgggcgg gatccacggc atcatatcga
     1261 tatcgggcgc ggacgcggga tcgctcgcaa attgcaccgg atcgagcccg agaatctcca
     1321 gggcatcggc gatctcgtcg gtgaatcccg ggtacatcat ccgctgatag gtcatcgcga
     1381 ggtcgacgac ggcatcccgg atcgaatcga gctcggatct cagggagtcg tccctggcga
     1441 tatcgatcag gaggcggcgc tcgatcccgc agaggaagat ccggatgaac tcgcgcggaa
     1501 tgtgtggatc cttgcgaccg ccggcgagcc aggcgaggta ttcggcgcgc cccagcccgg
     1561 aaagtgcttc atatgaagcc gtgactccga gcgacgggac accctgttcc agcgagaatc
     1621 cgatgtggag gtccggatcg atcagggacg gttccgaatg cggttccttg gtcgggctcc
//
LOCUS       DASTMD010000376         1857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1552765, whole genome shotgun sequence.
ACCESSION   DASTMD010000376 DASTMD010000000
VERSION     DASTMD010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552765"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..764
                     /locus_tag="VFQ54_05505"
     CDS             147..764
                     /locus_tag="VFQ54_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0164482.1"
                     /translation="MTLPTVRILLAEDQAMVRGALAALLSLEDDLEIVAELARGDEVV
                     ATASSIRPDVALLDIEMPGQDGLSVTRELTAALPACKVIILTTFGRAGYLRRAMENGA
                     VGFLLKDAPATELARAIRRVMAGERVVDPNLALTALSEGNNPLTDRERDVLAASKTGA
                     TIAEIANDLFLSQGTVRNHLSSAIQKLNVQNRIEAARVADEKGWL"
     gene            complement(947..>1857)
                     /locus_tag="VFQ54_05510"
     CDS             complement(947..>1857)
                     /locus_tag="VFQ54_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU0164483.1"
                     /translation="DSVRKNGLGLKGPITTPIGSGFRSVNVGLRQSLELYANLRPGKT
                     IKGVQSTFEDIDLVIVRENMEDTYAGVEFDTGTEEAAQVIREINKLSKKQVAADAAIT
                     IKMITPEGCRRIVRYAFDYARKNNRKLVTAVHKANIMKFTDGLFLRIAEEVAAENPDI
                     QFNDRIVDNMCMQLMQKPEQYDVLVMPNLYGDIVSDLVSGMIGGLGVAPGGNIGAKAA
                     VFEPIHGSAPSHAGKNEANPVAMILSGALMLRHLGEISSAEAVEGAVATVIGEGKDVT
                     YDLRADRDPSKATTTSGMADAIIAKL"
BASE COUNT          314 a          607 c          599 g          337 t
ORIGIN      
        1 ccgctcccag ggggagggtt ccggctctac gcccgcattc cgctcgcgac gtcaccggcg
       61 gccggcgaga accagtcgac gagcgaaacg gcccgcgcga ccgcgatccc atcgttcacg
      121 acgtcgcggt tcgaaggatc cgcgacatga cgctccccac tgtccggatt cttctcgccg
      181 aggaccaggc aatggtccgg ggcgcgttgg ccgccctgct ttcgctggaa gacgatctcg
      241 agatcgtcgc cgaactcgcc cggggcgacg aggtggtcgc gaccgcatcg agcatccgac
      301 ccgacgtggc cctgctcgat atcgagatgc cggggcagga tggcctgagt gtgacgcgcg
      361 aactcaccgc ggcccttccc gcatgcaagg tgatcatcct gaccacattc gggcgcgccg
      421 ggtatctccg gagggcgatg gagaacggcg ccgtcggctt cctgctcaag gacgcaccgg
      481 cgaccgagct cgcacgggca attcgccgcg tgatggcggg cgagcgcgtc gtcgatccga
      541 atctcgcgct gacggcactg agcgaaggca acaatccgct caccgaccgc gagcgggacg
      601 tgctcgccgc ctcgaagacg ggcgccacga tcgcggaaat cgcgaacgac ctcttcctgt
      661 cccagggaac cgttcgcaat catctctcgt cggcgatcca gaagctgaac gtccagaacc
      721 ggatcgaggc cgcgcgcgtc gcggacgaaa agggctggtt gtaggcgctt cacgggcata
      781 tcgaaggcgg gacatcaaag tccccgccct acgaacgaac gctatacact gggccgcagt
      841 cacgcgagat gccccactac cgcggcgaaa tcggtcgtga gtagggtggg acttctatgt
      901 cccacctctg gtcgcgattc tgcaactgcc cgttatggcg gtgaatctac agcttcgcga
      961 tgatcgcgtc agccatgccg gaggtggtgg tcgccttcga cgggtcgcgg tcggcgcgca
     1021 agtcgtaggt gacgtccttg ccctcgccga tcacggtcgc gaccgcaccc tcgactgcct
     1081 ccgcggacga gatctcgccg aggtgacgca gcatcagggc gccggagagg atcatcgcga
     1141 ccgggttcgc ctcgttcttc ccggcgtggg aaggcgccga tccgtggatc ggctcgaaga
     1201 cagccgcctt cgcgccgata tttccacccg gcgccacgcc gagtccgccg atcatgccgg
     1261 agacgagatc gctgacgatg tcgccgtaga gattcggcat cacgaggacg tcgtactgct
     1321 ccggcttctg catcagctgc atgcacatgt tgtcgacgat gcgatcgttg aactggatgt
     1381 ccgggttctc ggcggcgact tcctcggcga tgcggagaaa gagaccatcc gtgaacttca
     1441 tgatgttcgc cttgtggacg gcggtcacca gcttccggtt gttcttccgg gcgtagtcga
     1501 aggcatagcg cacgatgcgg cggcagccct cgggcgtgat catcttgatc gtgatcgcgg
     1561 cgtcagcggc gacctgcttc ttgctcagct tgttgatctc gcggatgacc tgggcggctt
     1621 cctcggttcc ggtgtcgaac tcgacgccgg cgtacgtgtc ttccatattc tcgcggacga
     1681 tcacgagatc gatgtcctcg aaggtcgact gcacgccctt gatcgtcttg cccggacgca
     1741 ggttcgcgta gagctcgagc gactggcgga gcccgacgtt gacgctgcgg aagccgctcc
     1801 cgatcggcgt ggtgatcggt cccttcagcc ccaggccatt cttgcgcacc gaatcga
//
LOCUS       DASTMD010000377         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2625143, whole genome shotgun sequence.
ACCESSION   DASTMD010000377 DASTMD010000000
VERSION     DASTMD010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2625143"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..537)
                     /locus_tag="VFQ54_05515"
     CDS             complement(46..537)
                     /locus_tag="VFQ54_05515"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0164484.1"
                     /translation="MPMNQAHMELCSSAEWAEHIEKTIVPWATGDLEFGDNVLEIGSG
                     PGLTTNVLQHKVPRLTASEIDPEMAAALATRFADVPNVTVIQADATNLPYPDDTFSTV
                     FSFTMLHHVPTVELQDKLLAEVARVLRPGGLFVGTDSLDSPGFRELHVGDICNVVNPL
                     TFE"
     gene            complement(687..1511)
                     /locus_tag="VFQ54_05520"
     CDS             complement(687..1511)
                     /locus_tag="VFQ54_05520"
                     /inference="COORDINATES: protein motif:HMM:NF016818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEU0164485.1"
                     /translation="MAATDTRFRTERFPMSFRRLEVKRIETITPGYVRIVAGGPELEG
                     FQSLDPDDHFKLILPNPGESEPVLPVQTEGGFAYPEDKPRPVMRDYTPRAVDAAAREL
                     SIDVALHEDGFASNWARQAKPGDVIGTAGPRGSHIVDYDAFDWFLVAGDETSIPSIAR
                     RLEDVPAGKPIFAFIEVDGAADEQPLAHSDGATITWVHRNGVPAGVSTALETAIRAFS
                     FPAGEGYQWYTGETTALREIRRYILNERGFDRQWVSFSGHWKYGQADFDHHQKIED"
BASE COUNT          290 a          521 c          557 g          341 t
ORIGIN      
        1 ccagacggtg aggtcgacgt cacggaaacc agccgtgcgc aggcgctatt cgaaggtcag
       61 cggattcacc acgttgcaga tgtcgccaac gtggagttcg cgaaaacccg ggctgtcgag
      121 cgaatcggtt ccgacaaaca acccgcccgg acgaaggacg cgggcgacct cggcgagaag
      181 cttgtcctgc agctcgaccg tcggcacgtg gtgcagcatc gtgaaactga agaccgtgct
      241 gaaggtgtcg tccgggtagg gcaggttcgt ggcgtcggcc tggatgacgg tgacgttcgg
      301 gacatcggcg aagcgggtgg cgagtgcggc cgccatctcg ggatcgatct cggaagcggt
      361 gagccggggg accttgtgct gaaggacatt cgtcgtcaga cccgggccgg agccgatttc
      421 gagaacgtta tccccgaatt cgaggtctcc cgttgcccac ggcacgatcg tcttctcgat
      481 atgctctgcc cactccgcgc tcgagcagag ttccatgtgc gcctggttca ttggcatcgg
      541 acgagccctt ctttctctga ttcccggatt cggcgccctg cttcacccgc aaggtgctgc
      601 cattgcgttc gccccgttat atcgcgccgc gcgcagtggg tcacgcgggg tgacccggcg
      661 caacgtggac gcgggcgaag cgccccctag tcctcgatct tctggtggtg atcgaagtcg
      721 gcctgtccgt atttccagtg gccggagaaa ctcacccact gccggtcgaa gccacgctcg
      781 ttgagaatgt agcgacggat ctcccggagg gcagtggtct cgcccgtata ccactggtaa
      841 ccctcgccgg ccgggaagga gaaggcccgg atcgccgttt ccagtgccgt cgagacgccg
      901 gcgggaacgc cattgcgatg gacccaggtg atggtcgcac catccgaatg ggcgagcggc
      961 tgctcgtccg ccgcgccgtc gacctcgatg aaggcgaaga tcggtttgcc ggcaggaacg
     1021 tcctcgaggc gccgtgcgat cgaggggatc gacgtctcgt cgccagcgac gaggaaccag
     1081 tcgaaggcgt cgtaatcgac gatgtgcgat ccgcgcggtc ctgccgtccc gatcacatcg
     1141 ccgggcttcg cctgccgtgc ccagttcgat gcgaatccgt cttcatgcag cgcgacgtcg
     1201 atgctgagct cacgggccgc cgcatcgacg gcgcgcggcg tgtaatcgcg catcacggga
     1261 cgcggtttgt cttccggata ggcgaatcca ccctccgtct ggacaggaag gaccggttcg
     1321 gattcgcctg ggttgggaag gatcagcttg aaatgatcgt ccggatccag tgactggaat
     1381 ccttccagct ccggtccgcc ggcgacgatg cgcacgtagc ctggtgtgat cgtttcgatc
     1441 cgctttacct cgagacgtcg gaaggacatc ggaaaccgct cggttcggaa gcgggtatcg
     1501 gttgcggcca tgtgaattct cctgcgccga gcctacttga cgcgcgtcgc cggtgtcccg
     1561 tcgggatagc tcttcaggat atcctcgatc ttgccaacga agttgatcgc gtcgccatag
     1621 cttcccggga tgatgttggt gatcgcgtag acgtgcttgt tcttgctttc cttcagcgcc
     1681 ttgaagaggt cctggttcat catcgcctg
//
LOCUS       DASTMD010000378         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_839178, whole genome shotgun sequence.
ACCESSION   DASTMD010000378 DASTMD010000000
VERSION     DASTMD010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839178"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFQ54_05525"
     CDS             complement(<1..516)
                     /locus_tag="VFQ54_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164486.1"
                     /translation="MDRDAVWQETSSHDGRGHDIGFESEILYRPDIQTLFITWLPPKD
                     ELDGVPETLAYSLDPDQINGLVRAMTSAPYRESAYFARIRGRQVTDTGLEARIAALEA
                     EMETMKQELKDARARVAGLEKKVSEQGNVLSQLFEGHTRMQRDFAKLKANANSTAAAL
                     FPRVLALEAEMA"
     gene            complement(885..1196)
                     /locus_tag="VFQ54_05530"
     CDS             complement(885..1196)
                     /locus_tag="VFQ54_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164487.1"
                     /translation="MPTRDLTYIERIAQDVYEIASATEQPYLADEASLWLGYAVLVLT
                     KGTETTSADVHEAWSAWAAVRHNGNHRSLIPFDELEPKIQAYDDLYRDAIHAVAKRRA
                     A"
     gene            complement(1178..1408)
                     /locus_tag="VFQ54_05535"
     CDS             complement(1178..1408)
                     /locus_tag="VFQ54_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164488.1"
                     /translation="MAHIRIEQNKDEFHPRVLIAETGERIKGIVSVAYHSDQEQFATL
                     SIELAAMLHEVDITLDVDVEVKQEEPRCQPVT"
BASE COUNT          308 a          510 c          493 g          384 t
ORIGIN      
        1 tgccatctcg gcttcaagag caagaacgcg cggaaacaag gcggcggcag tgctgttcgc
       61 gttcgccttc aatttggcaa agtcccgctg catgcgtgtg tgtccctcga agagctgaga
      121 gagtacgttc ccctgttccg acaccttttt ctcaagcccg gcgactcgag ctcgtgcatc
      181 cttcagctct tgcttcatgg tttccatctc ggcttcaagc gcggcgatgc gggcttcaag
      241 gcccgtgtcg gtcacctgtc gcccacgaat ccgggcgaag taagccgact cgcggtacgg
      301 tgccgacgtc atagcccgga ccagtccgtt gatctggtcc ggatcaagtg aatacgccag
      361 cgtctccgga acaccgtcca gttcatcctt aggcggaagc caggtaatga agagcgtctg
      421 gatgtcggga cgataaagga tctcgctctc gaacccgata tcatgccctc ttccgtcatg
      481 agaggacgtt tcctgccata cagcatccct gtccatcacg tcacctcacc cggcctgtta
      541 tcgatccggg attccgtctc gcctgttcct tgagcttatc cgtctcggtt gtgccaatgg
      601 tgatcggcgc gaacacctga tcgtgaacct cgatgacgcc acggcctcca cacgatcggc
      661 aggtctcggg attcgtagac gatgacgacc atgtgttcga cgtcgaggaa tagaaaccgt
      721 tcgggacgat gcccttgccg tagcagacgg gacatcgatg gacgctgctc atcgtcgtcg
      781 tcctttcgcg aactttgctg cacgccgccg atcatgacgg ttcgggccac ggtggagttg
      841 gatgtaaggc ggactcggaa accatccaag aacgggccat cccatcacgc cgccctccgt
      901 tttgccaccg cgtgaatcgc gtcccggtac aggtcgtcgt aggcttggat cttcggctcc
      961 aactcgtcga acgggatcag cgagcggtga ttgccgttat ggcgcaccgc agcccaagcg
     1021 ctccacgctt cgtggacgtc ggcggaggtc gtctcggtgc ccttggtgag gacaaggacg
     1081 gcgtacccga gccagagcga tgcttcgtcg gcgaggtacg gttgctctgt cgccgaggcg
     1141 atctcgtaca cgtcctgcgc gatgcgttcg atgtaggtca ggtcacgggt tggcatcgtg
     1201 gctcctcctg cttcacctca acgtcgacgt caagcgtgat atcgacctcg tgcaacatgg
     1261 cggcgagctc gatcgacaag gttgcaaact gttcctggtc tgagtggtag gcgacggaga
     1321 cgattccctt gatgcgttcg ccggtctcgg cgatgagcac gcgcgggtgg aactcgtctt
     1381 tgttctgttc gatcctgata tgggccacgg ttgctccgtt cacgattgac gtgggtggta
     1441 aactaaaagc aggtcaagtg atcggtcgag gtagaaaaat cttgcatcta tgaccgttgg
     1501 gttatgcggg gaacgcgagt cccgccgtcc gatcactgcc cgtcctccat caacaaccga
     1561 ctatccccct ttgctttatc cttcgccttc tcggaccgtt tggtccgctg gattcgtgcg
     1621 ctcttgaccc ggtgcaatcc ctcgccgggg aagaactcga cgtggctcac taggtcatgt
     1681 gcaggatcca ccgat
//
LOCUS       DASTMD010000379         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2803373, whole genome shotgun sequence.
ACCESSION   DASTMD010000379 DASTMD010000000
VERSION     DASTMD010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2803373"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1905
                     /gene="dxs"
                     /locus_tag="VFQ54_05540"
     CDS             <1..1905
                     /gene="dxs"
                     /locus_tag="VFQ54_05540"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEU0164489.1"
                     /translation="DTIRQPGDLNGKSLRELNTLAAEIRAELIDIVSQTGGHLGASLG
                     TVELAIALHYVFESPRDKLVWDVGHQAYVHKILTGRRDRFPTLRQENGMSGFLSRDES
                     AHDAFGAGHASTSISAALGMCVAEQRKPKQSRGRAVAIIGDGALTGGMAFEGLNNAGN
                     LNYPFMVVLNDNEMSIAPNVGALSKYLDRMRSDKGYNRAKDELADKIAKLPKGELLVE
                     LGKRTKDSFKEFIYHSMIWEELGFTYIGPVDGHDIKATIEALRRGADIEGPSFVHILT
                     KKGKGYEPALTDKERSHAISAPSKPQPVGSPPVAPKYQDVFADTLIELGATNDRVYAI
                     TAAMPGGTSLGKFGARFPERFFDVGIAEQHAVTFAAGLASEGLRPVCGIYSTFLQRAY
                     DQIIHDVCIQKLPVIFGMDRAGFAGDDGRTHHGVFDLSYLRCLPNMTIMAPKDENELR
                     HMLATAVDHTEGPTAVRFPRGAGIGVDMSEPLKSLPFGKAEVLRDGKDVAILAVGSMV
                     ANAELAADELANDGITATVVNVRFVKPFDEELVRALAISHGAIVTIEESAAAGGFGSG
                     VLEFLSAEGLSVPTKILGVPDHFFEQASQNRLRELAGLGVKDIVNASQQVLAARRLAG
                     SRKDAPVVAS"
     gene            1892..>2108
                     /locus_tag="VFQ54_05545"
     CDS             1892..>2108
                     /locus_tag="VFQ54_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164490.1"
                     /translation="MLHPEESNLIDRTVQSGDDLSQGEGPYDLGHPREDTTEEQVPTE
                     PSSLEDLTLLWRSAWPDLTDEQVRLLAG"
BASE COUNT          395 a          728 c          651 g          334 t
ORIGIN      
        1 gacacaatca ggcagcccgg tgacctcaat ggaaaatccc tccgcgaact gaacaccctc
       61 gccgccgaga ttcgcgccga actcatcgac atcgtttccc agaccggcgg ccacctcggc
      121 gccagcctcg gcaccgtcga gctcgcgatc gccctccatt acgtcttcga atctccacgc
      181 gacaagctcg tctgggacgt cgggcatcag gcctacgtcc acaagatcct caccggtcgc
      241 cgcgaccgct tcccgacgct ccgccaggag aacgggatgt ccggcttcct gagccgcgac
      301 gagagcgccc acgacgcctt cggcgccggg cacgcctcga cctcgatcag cgccgcactg
      361 ggaatgtgcg tcgccgaaca gcgcaagccg aagcagtctc gtggccgcgc cgtggcgatc
      421 atcggcgacg gcgccctcac cggcggcatg gccttcgagg ggctcaacaa tgcgggcaat
      481 ctcaactacc cgttcatggt cgtcctcaac gacaacgaga tgtcgatcgc cccgaatgtc
      541 ggcgcgctca gcaagtacct cgatcgcatg cgaagcgaca aggggtacaa ccgggccaag
      601 gatgagcttg ccgacaagat cgcgaagctc ccgaagggcg aactgctcgt cgagctgggt
      661 aagcgaacga aggactcgtt caaggaattc atctaccact cgatgatctg ggaagaactc
      721 ggcttcacct atatcggccc cgttgacggg cacgacatca aggcgacgat cgaggccctc
      781 cgccgcggcg ccgacatcga gggaccgagc ttcgtccaca tcctgacgaa gaagggcaag
      841 ggctacgagc ccgcgctcac cgacaaggag cgcagtcacg cgatcagcgc cccctccaag
      901 ccgcagccgg tcggttctcc cccggtcgcg ccgaagtacc aggatgtctt cgccgacacc
      961 ctgatcgaac tcggcgccac aaacgaccgc gtctacgcga ttacggccgc gatgccgggc
     1021 gggacctcgc tcggcaagtt cggcgcgcgg ttcccggagc gcttcttcga cgtcgggatc
     1081 gccgagcagc acgcagtgac cttcgccgcc ggtctcgcct cggaaggctt gcggcccgtc
     1141 tgcggcatct actcgacgtt cctgcagcgc gcctacgacc agatcattca cgatgtctgc
     1201 atccagaagt tgccggtcat cttcggcatg gatcgcgcgg gcttcgccgg cgacgatgga
     1261 cgcacccacc acggcgtctt cgatctcagc tacctgcgct gcctgccgaa catgacgatc
     1321 atggcgccga aggacgagaa cgagctgcgg cacatgctgg cgacggcagt cgaccacacg
     1381 gaaggtccga ccgcggttcg cttcccacgc ggcgccggga tcggcgtcga catgagcgag
     1441 ccactgaagt cgctcccgtt cggcaaggcg gaagtcctcc gcgacggcaa ggatgtcgcg
     1501 attctcgccg tcggctcgat ggtggcgaat gccgagctcg cggcggacga gcttgccaac
     1561 gacggcatca cggcgaccgt cgtcaatgtc cgtttcgtga agccattcga tgaggagctg
     1621 gttcgcgcgc tcgcgatctc ccacggcgcg atcgtcacga tcgaggagag cgcggcggcc
     1681 ggcggattcg gttccggcgt cctcgaattc ctctccgccg agggactcag cgtcccgacg
     1741 aagatcctgg gcgtgccgga tcacttcttc gagcaggcct cgcagaaccg cttgcgcgaa
     1801 ctcgccgggc tcggcgtgaa ggacatcgtc aacgcgtccc agcaggtgct ggcggcacga
     1861 cggctggcgg gctcacgaaa ggatgcgccc gttgttgcat cctgaggaat ccaacctgat
     1921 cgatcgcacg gtgcaatccg gggacgacct ctcacaggga gagggtccct acgatctcgg
     1981 gcatccgcgt gaggacacga cggaagaaca ggttccaacc gagccgagtt cgctcgagga
     2041 tctcacgctc ctctggcggt ctgcctggcc ggacctgacc gacgagcagg ttcgcctcct
     2101 cgcgggct
//
LOCUS       DASTMD010000380         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_927635, whole genome shotgun sequence.
ACCESSION   DASTMD010000380 DASTMD010000000
VERSION     DASTMD010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927635"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="VFQ54_05550"
     CDS             complement(<1..1412)
                     /locus_tag="VFQ54_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEU0164491.1"
                     /translation="MIPQPTRIRSLTEAPFTLTAATRIVAAPELARSASWFTTELRRL
                     TGLEIATRDTESPAIRLSLVVGDAELATLPPTGGVRADDADPDAERYALDIRESGISL
                     HAATAEGIFRGLTTLLQIVVTTEAGSDRSRTLPAVNILDTPRFGWRGLSFDVVRTFYT
                     PDEVRRVIDLLALYKANVLHLHLTDSEGWRLQIDSWPLLTEIGSKGAAAGRSGGFYTK
                     DEYRDIVQYAADRFITIVPEFDMPGHTAAIYEAYPELAGNGIDDPAAGLAQGRWFQVM
                     HPDHPRIFAFITEVLTEIAELTPGAYIHIGGDEALGMDEELYARFIERAMPIVRDIGR
                     KPLGWQETARAGLTSGDIAQLWISPRVGEGIDLTEIDFGNLPEAVDFPPEQAAQLIEA
                     VMEMLKIAALDLDKALDQGANILVSQSTEAYLDTQYLESPSDPDQEADWKRLGMPFYP
                     KSTVAEFFSWDPASLRPTLK"
BASE COUNT          336 a          646 c          684 g          376 t
ORIGIN      
        1 tccttcagcg tggggcgaag tgacgcggga tcccagctga agaattcggc aaccgtcgat
       61 ttcggataga agggcatgcc gaggcgcttc cagtccgctt cctggtcggg atcagacggc
      121 gattccaggt attgggtgtc gaggtaggcc tcggtcgatt gcgaaacgag gatgttcgcg
      181 ccctggtcga gcgccttgtc gagatccagc gccgcgatct tcagcatctc catgaccgct
      241 tcgatcagct gggctgcctg ttccggtggg aaatcgaccg cttccgggag gttgccgaag
      301 tcgatctcgg tcaggtcgat gccttcgccg acgcgggggc tgatccagag ttgggcgata
      361 tccccgctcg tcagcccggc gcgggccgtc tcctgccagc cgagcggctt gcggccgata
      421 tcccgcacga tcggcatcgc gcgctcgatg aagcgcgcgt agagttcctc gtccatgccg
      481 agcgcctcgt cgccaccgat gtggatgtag gcgccgggtg tgagttccgc gatctcggtc
      541 aggacctcgg tgatgaaggc gaagatccgg ggatggtccg ggtgcatgac ctggaaccag
      601 cgaccctggg cgagaccggc ggcaggatcg tcgatgccgt tgccggcgag ctctggatag
      661 gcttcgtaga tcgcggcggt gtggccaggc atgtcgaatt ccgggacgat cgtgatgaag
      721 cggtcggccg cgtactggac gatgtcccgg tattcatcct tggtgtagaa accacccgat
      781 cgtcccgctg ccgcaccctt actcccgatc tcggtcagca acggccagga atcgatctgg
      841 agacgccatc cctcggaatc ggtcagatgg agatgcagga cgttcgcctt gtagagcgcc
      901 aggagatcga tcaccctcct gacctcgtcc ggcgtgtaga aggtccgcac gacatcgaag
      961 gagagcccgc gccagccaaa acgtggcgta tcgagaatat tcactgccgg gagcgtccgt
     1021 gatcgatccg agcccgcctc ggtcgtgacg acgatctgga gcagcgtcgt caggccgcgg
     1081 aagatcccct cggccgtcgc ggcgtgcagg gagattccgc tctcgcgaat atcgagggca
     1141 tatcgctcgg cgtcgggatc ggcatcgtcg gcacggacgc cgccggttgg gggaagcgtc
     1201 gccagttcgg catcgcccac cacgaggctg aggcgaatcg cgggcgattc ggtatctcgc
     1261 gtggcgatct cgagacccgt caacctgcgc aactctgtcg tgaaccacga cgccgagcgg
     1321 gccagctcgg gggcggcaac gatccgcgtc gccgccgtga gcgtgaacgg ggcttccgtg
     1381 agcgaccgga tacgggtggg ttgcgggatc acgttcaatc tgaccctcct tctcgttgcc
     1441 tgcgtatgcg acagatgtct tcgagaagcg tacaccgcgc ccgcccacca cgatccccat
     1501 gactgggcgt acccggcgtg cccggtcatg gggccgccat tcgtataatc gcggcacgcg
     1561 agttgcgtag tatgtggtgc gctcccgacg atgtggtcgt gggcgcgagt ggacatcgtt
     1621 gacacaataa gtggcctaca cgagccgggg ttctcagcga taagctgatt caagctgaac
     1681 tgaccccggc tcgaccatat ctgatcccca ggacattccc ctgcgacgat cgggccgtct
     1741 cctccagcga aatccggatt cgtcgatacg tttggcagcg ccggggttgg tagggcgggg
     1801 tcttataccc cgccgcaggg cggaacccct ttccacaacg ccgcgtccgg ggccccgtgg
     1861 accgacgtgt tgcccatcca ttcccacggc gttcttcgcc gcctccccgt tggcgacgga
     1921 tgccttcggc gcgcgaacgc cgatccgctt ccgggagcgc gtcgcgcgcg ggttcccgtt
     1981 ggcgtccgcc ggagcccgcc ctgagctcgt cgaacggggc ggagggcgag cacgaggctc
     2041 gc
//
LOCUS       DASTMD010000381         1514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1732420, whole genome shotgun sequence.
ACCESSION   DASTMD010000381 DASTMD010000000
VERSION     DASTMD010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732420"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VFQ54_05555"
     CDS             <1..461
                     /locus_tag="VFQ54_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164492.1"
                     /translation="MREIYETSHVPLYLDRNAQQLLYEGRASYRHLNLQQEAVWHWGG
                     DSLVFPWRGDRIMNTLMILLAQAGINVSSEGIAIACKDASAQQVRRVLRELAGSPMPD
                     PIELASRVAVKEKEKFDGFLGDELLTAGYASRDLDLPGTWQTLRDLEFRT"
BASE COUNT          281 a          538 c          417 g          278 t
ORIGIN      
        1 agatgcggga gatctacgag acgtcacacg tcccgctcta tctcgacagg aacgcgcagc
       61 aactgctcta cgaaggccgc gccagctacc gtcacctgaa tctccagcag gaggcggtct
      121 ggcattgggg cggtgactcg ctcgtctttc cgtggcgggg tgaccggatc atgaacacgc
      181 tgatgatcct gcttgcccag gctggcatca acgtcagttc ggagggaatc gcgatcgcct
      241 gcaaggacgc ctccgcacag caggtgcggc gcgtactccg cgagctggcc ggctccccga
      301 tgccggatcc gatcgagctg gcttcccggg tcgcggtgaa ggagaaggag aagtttgacg
      361 gcttcctcgg cgacgagctc ctcaccgccg gctacgcctc ccgcgacctc gacctcccgg
      421 gcacctggca aaccctccgc gacctcgaat tccgcacctg acccggaggt tcgtgcagcg
      481 gtcgttcgta ccgacggacg gcaatgtcca tccgtccggc aatgccggac acgatcgctc
      541 gggccatcaa gcgccgtgac tcccaatgtc ccatgtccca ttcccgtcat ttcgagcctg
      601 tcgagaaatc cccgcgcgaa gcgcatgccg ggcaacgccc ggcctgatcg tcccatgaac
      661 gatgcctcgc cgctcaccat catccgaaga atcgtcgtcg cgacccttgt gaccgccggg
      721 cgatatgaaa ttccgcccta caatccccgg cctcaccgca catcgatcac cttcctcaag
      781 gctgggggcg ccgcgcccaa gtccgctttg cggcggggta tgaaaccccg ccctaccaac
      841 cccagcgccg ccaggcgtgc cgatggcttc ggatttcgcc ccataatcaa cgaacggacg
      901 acgcgattga cgagcttcgc ttgacgcaca aagtacacgt cgatcgcttc cgaaaggacc
      961 aagcatgacg accaatacgt ggtgcgcccc aggatcgcgg agcgaaccaa cgcgctgcgg
     1021 cagggcgcga atgtcgcgat cgagggatca ggattgactt ctcgaagtcc ggtcgctaag
     1081 gtaggactcg gtcggaagcg tcttgcttca gggccgtcgc attcgttctc gctccctgga
     1141 ggttcattca catgtccgca gctacagctc cccgcgcatg ctggatgagc atttccctcc
     1201 tcggctagaa ccgtcacgtc actcccggtg acgcaccgaa ggcacgactt ctcctccgca
     1261 tcgcgcaatg aatcgtgacc cagcagggat tcctgccggg cccgtctcat gctccacgcg
     1321 ttccggagaa tcccatacgt gtcgccttca tccgtttcct cccgcaccct gaaccgttac
     1381 acgcggttgc ttacccacta ccttcacccg catcggctgc agatgatcct gctgatgctg
     1441 atcctctgcg ccacgatcgc gatccagatc gcgacgccac tcgtcgccag ccgcttcatc
     1501 gatgacgcca cctc
//
LOCUS       DASTMD010000382         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2713898, whole genome shotgun sequence.
ACCESSION   DASTMD010000382 DASTMD010000000
VERSION     DASTMD010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2713898"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1200
                     /locus_tag="VFQ54_05560"
     CDS             97..1200
                     /locus_tag="VFQ54_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU0164493.1"
                     /translation="MTSEIIARGAIARQVNAPASIEEFVVPSPGPGEARIQILASGVC
                     HTDLSCKNGLFGTDGFPFLLGHEGAGIVESVGEGVTNVAAGDYVILAWRAPCGECRFC
                     RAGKPNLCSASLNATQKMHALSDGVELNAVLGIGTFCTHTLVHAKQCVPVSHELPPAQ
                     MSLIGCGVMTGVGAALYSAKVEPGSSVAVFGCGGVGDSVIMGARIAGATKIIAVDLDD
                     RKLAWAKEFGATDLVNPRDGDPVEKIKELTGGHGVNFSFEAVGRADTLSQAIFSRDLA
                     GTCVMIGVAGPSAVLPELPLGKFFDLGGSLRVSWYGDCLPTRDFPMLADWYEQGRLDL
                     DRVVTKIITLDEVDEAFEAMHRGETLRSVIAFD"
     gene            complement(1609..>2812)
                     /locus_tag="VFQ54_05565"
     CDS             complement(1609..>2812)
                     /locus_tag="VFQ54_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0164494.1"
                     /translation="FRAPYVSHTPLEPKASLVQIDGDKVNVWTSTQGPFQIQQAVSDL
                     LKVPLDNVLVTPLMSGGAFGSRSSAVAELEAAQLAKGIGKPVIIQWTRQEELQWGRFR
                     PAAQVEISAGLDANGAPVGWKYDLYSAGYFPEGAEMATSASANASASVTDVYDLPQAK
                     TTFYQGASPLPVAHWRGNGTSYNVFAYEGVIDQLAEAAGKDPVSFRASLLDKNPRMKA
                     VLDAVVAKADWKPGVGSTGQGIGVALAFADETYVAEVAKVQVDADSGQVKILHFDVAI
                     DAGLIVNPEAATHQAEGSVIFSLSPTIREAAAFDNGKVTNDTWAQYQPIKFSEVPTID
                     VIFVEDKTQPMGGMGEPAVAPVPAAVANAIYDAVGVRLFELPFTPDRVLAAIQAKKNG
                     TPVASPSA"
BASE COUNT          454 a          878 c          987 g          493 t
ORIGIN      
        1 catacgatga ctaggtggta ccgtggatgc gattgccaca gagagccgga ttgaaacgtg
       61 ccaggcgcgt cgttgcttgc catcaaggag gatcgaatga cttcggagat catcgcgcgc
      121 ggagcgatcg cccgccaggt aaatgcgccg gcatccatcg aggagttcgt cgtaccgagc
      181 cccgggccgg gtgaggcgcg catccagatc ctcgcgagcg gcgtctgcca caccgatctc
      241 tcctgtaaga acggcctctt cggtacggac ggattcccgt tcctgctcgg tcatgaagga
      301 gcgggcatcg tcgagagcgt cggcgaaggc gtgacgaatg tcgccgcggg tgattacgtg
      361 atccttgcct ggcgcgctcc ctgcggcgag tgtcgcttct gccgcgcggg caagccgaat
      421 ctctgcagcg cgagtctcaa tgcgacgcag aagatgcatg cgctcagcga tggcgtcgaa
      481 ttgaacgccg tcctggggat cgggacgttc tgtacgcata cgctcgtgca cgcgaagcag
      541 tgcgtgccgg tgagccacga gctgccgccc gcgcagatga gcctgatcgg ctgcggtgtg
      601 atgaccggtg tcggcgccgc gctctactcc gcgaaggtcg agccgggatc ctcggtcgcg
      661 gtcttcggct gcggtggtgt cggcgattcc gtgatcatgg gtgcgcggat cgcgggagcg
      721 acgaagatca tcgctgtcga tctcgacgac cggaagctcg cctgggcgaa ggagttcggc
      781 gcgacggatc tcgtcaatcc gcgtgacggc gatcccgtgg agaagatcaa ggagttgacg
      841 ggcggtcacg gcgtcaactt ctcgttcgag gcggttggac gtgcggacac gctctcccag
      901 gcgatcttct cgcgcgacct cgccggcacc tgcgtgatga tcggcgtcgc cgggccgtca
      961 gcggtcctgc cggaactgcc gcttggcaag ttcttcgatc tgggtggttc gctccgggtc
     1021 agctggtacg gagactgcct ccccactcgc gacttcccga tgcttgcgga ctggtacgaa
     1081 caggggcggc tcgatctcga tcgcgtggtg acgaagatca tcacgctcga cgaggtcgat
     1141 gaggcgttcg aggcgatgca ccgcggagag acgctgcgtt ccgtgattgc tttcgactag
     1201 gcaacgaagg agccgcgggc gaggcatcga cccgcgacgc gtgcggaggc gttggggttg
     1261 gtagggcggc gctttatgcc ccgccgcgag gcgagggtgc gcgcaacgca cctcgcatgc
     1321 gatgtcgttt tgcggcaagg cacaaggcct tgccctacag accccggatt gtccgcgatc
     1381 cggcgagcga tctgaagtgc atcgcaagtc ccgcgcacga ggcggcaagg cgtgggatcc
     1441 aacgacctcc tgcgttcgac ctctcggcgc cgacgttcat atccggctgt gccggatggg
     1501 tcacacgggg tgacccacca ggggtgccaa atcggtccgc cggggacagt cgtaggcaaa
     1561 gaggggagga cggccacaag cggccgtcct cccctggatg atcgggacct acgccgatgg
     1621 cgaggcgacc ggcgtgccgt tcttcttcgc ctggatcgcc gcgagaacgc ggtcaggcgt
     1681 gaacggaagc tcgaagaggc gaacgccgac cgcgtcatag atcgcgttgg cgaccgccgc
     1741 cggaaccggg gcgacggcgg gctcgcccat gccgcccatc ggctgcgtct tgtcctcgac
     1801 gaagatcacg tcgatcgtcg gcacctcgga gaacttgatc ggctgatact gggcccacgt
     1861 gtcgttcgtg accttgccgt tgtcgaacgc cgcggcctcg cggatcgtcg ggctcagcga
     1921 gaagatcacc gacccctcgg cctggtgcgt cgccgcctcg ggattgacga tcaggccggc
     1981 gtcgatcgcg acatcgaagt gcaggatctt gacctgaccc gagtccgcgt cgacctggac
     2041 cttcgcgact tccgcgacgt aggtctcatc ggcgaacgcg agcgcgacgc cgatgccctg
     2101 accggtggaa ccgacgcccg gcttccagtc tgccttggcg acgacggcgt ccaggaccgc
     2161 cttcatgcgg ggattcttgt cgaggagact ggcgcggaag ctgaccgggt ccttgccggc
     2221 ggcctcggcg agctggtcga tcacgccttc gtacgcgaag acgttgtaag acgtgccgtt
     2281 accgcgccag tgggcgaccg gcagcggcga tgcaccctga tagaaggtgg tcttcgcctg
     2341 cggcaggtcg tagacgtcgg tgacgctggc gctggcattg gccgaagcgc tggtcgccat
     2401 ctcggctccc tccgggaagt agccggcgct gtagagatcg tacttccagc cgaccggggc
     2461 cccgttggcg tcgagcccgg cgctgatctc gacctgggcg gccggacgga agcgtcccca
     2521 ctggagttct tcctggcggg tccactggat gatcaccggc ttgccgatgc ccttcgcgag
     2581 ctgcgccgcc tcgagctcgg cgaccgcgct cgagcggctg ccgaaggcgc caccggacat
     2641 cagcggggtg acgaggacgt tgtccagcgg caccttgagc agatcggaga cggcctgctg
     2701 gatctggaac ggaccctggg tcgaggtcca gacattcacc ttgtcgccgt cgatctggac
     2761 gagcgaagcc ttcggctcga gcggcgtgtg gctgacgtac ggggcgcgga ag
//
LOCUS       DASTMD010000383         6583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1823305, whole genome shotgun sequence.
ACCESSION   DASTMD010000383 DASTMD010000000
VERSION     DASTMD010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823305"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..481)
                     /locus_tag="VFQ54_05570"
     CDS             complement(53..481)
                     /locus_tag="VFQ54_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU0164495.1"
                     /translation="MSRLNLKAIGVLFIAIVTALSVVGVNAAAQSGTPSASPSASPSA
                     SPAASPSAGASFEVATVDLAFQPNTLTIPANTDVKITVTNKGVLQHDFTIDELGVKTK
                     LLNGGETDTVTINAKPGTYQFYCSVPGHKEAGMVGTLTVK"
     gene            complement(767..3199)
                     /locus_tag="VFQ54_05575"
     CDS             complement(767..3199)
                     /locus_tag="VFQ54_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU0164496.1"
                     /translation="MSSFPDGQDLATPPNPVAPNRISVVHFPQSERVFDVPAPLHPLV
                     GRVSILAALVERISRPDVRLMTVTGPGGVGKTRLALEAAHGLTGRYERIHFIPLASIV
                     DPALLPPTLVRGAGAGDEPNVGWVQRIASRAREQRVLLILDNLEQISDAGSVIAELIA
                     GSARIDVLATSREPLHVDGEHEFLLPCLELPDRTPVAADLAQSEAVALFVARVQRFDP
                     DFALTGRSAPVILEVCRLLDGLPLAIELAAARMKVLSPEALRERLRDHIDIDVLAGGP
                     RNQLAHQQTMRDTIEWSYRLLSPSEQRMLRWLSVFSMAFPFSAVETICARADGSAGSD
                     AIELVQSLVEKSLLRRVEGERGEPAFFMLATIRSFARDLAKAEGEEDALRTGHAAWCM
                     RLVEGYWSVPVVDFIPPLVLGRIDAQFNDLQRALEWFDRQGDATSLLRMTAQLAPYWT
                     LRSFRAEGSSWLRRALSQPGIEDVPMDLRAGAYLASAALARTQGDLGAAEAWCRQSLA
                     LYRKIGDPMREAAVLNLLGAVSRAQGDFVTATQVCEESLAIFGNYDDPSWTALLNCNL
                     GVIAFWRGKTREARAYVEAAARGYRAAGNEWGLTFTLMSLGLIRCAQGDPRAASEAVT
                     EGLRLARAVESRECLIDGIAATAVIAVAAGAFGLAATFFGSVEALCRTIDYALEQPEQ
                     QHYGVAAKSARQAIGSESFARAFDRGRGFEFAAAVDEAVAWLGRITTSGAPGASAVSG
                     VAGPMVAAELTSREHQVLALLLQRFTDREIAETLFISTRTVSRHVSNIFDKLGVRSRR
                     EAAQLFENAPPR"
     gene            3464..4129
                     /locus_tag="VFQ54_05580"
     CDS             3464..4129
                     /locus_tag="VFQ54_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0164497.1"
                     /translation="MRVLVVDDEEALADAVARGLRQANLAVDIALDGESALMKAEVTP
                     YDVVVLDRQLPDVHGDDVCRQLVAFETPPRILMLTAAGDVHSRIEGLAIGADDYLPKP
                     FVMGELIARIRALHRRPNIAIRPVLTIGDLVVDSSRQAATRAGRELSLTRKEFGVLEE
                     IARANGAIVSSEELLERVWDEAADPFTNAVRITVMTLRRKLGEPGMIETVVGSGYRLC
                     SQE"
     gene            4114..5352
                     /locus_tag="VFQ54_05585"
     CDS             4114..5352
                     /locus_tag="VFQ54_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0164498.1"
                     /translation="MLAGIAARLHLRWTVRLRLTLTYTALFVVTGALLLVLLYVLLSN
                     ALAPPTPRTGIPQGSGPITNISRGNTAADQADDTLNDQLDETRREERSAALQQVQVQA
                     TIALLLMSVASFVLGWIVAGRVLRPIRQITAHAQHASEATLNERIDLRGPPDELTNLA
                     NTIDGMLDRLQSAFESQRDFAAKASHELRTPLAIIGAEADIALDAPDASERERTTALA
                     IRNATNRSERLIDGLLMLARSESTMRDNARIDLAEVAGDIAGELVGEADIAGVAIDLQ
                     LDSAFVLGDRTLLERLVGNLVENGIRYNIRGGWLRLHVRRDGDRSVVIVENSGNERPI
                     GNVNELFKPFQRGGGATSNRRERTTGFGLGLAIVQSVAEVHAATIVTTARQDGGLRVE
                     VRFPAAPAPARLPTRNPQWI"
     gene            complement(6215..>6583)
                     /locus_tag="VFQ54_05590"
     CDS             complement(6215..>6583)
                     /locus_tag="VFQ54_05590"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU0164499.1"
                     /translation="DGYPDDHAIVPHGISVILTAPAIFRWTAAANPAKHLEAARLLGV
                     DTRGAAPEDGGELLAGAVIDLMHRVEMPNGLNGVGITLDDMPALVKGTLPQTRLTQLS
                     PRPSSEDDYRDLFAASMSIW"
BASE COUNT         1180 a         2269 c         2089 g         1045 t
ORIGIN      
        1 ggaggtcttc tcgtgctctc gcgagcgaat taccgatgtg actgacgtgg tcttacttga
       61 ccgtcagcgt gccgaccatg ccggcttcct tgtgacccgg aaccgagcag tagaactggt
      121 aggtgcccgg cttcgcgttg atcgtcaccg tgtcggtctc gccaccgttg agcagcttgg
      181 tcttcacacc gagctcatcg atcgtgaagt cgtgctgaag gacgcccttg ttcgtgaccg
      241 tgatcttgac gtcggtgttc gccgggatcg tcaacgtgtt cggctggaac gcgaggtcga
      301 ccgtcgcgac ttcgaacgac gcaccggcgg acggcgacgc cgcgggagag gcggacgggc
      361 tggccgacgg cgacgcgctc ggggtgccac tctgggcagc cgcgttcacg ccgacgacgc
      421 tcagggccgt cacgatagcg ataaacagga caccgatagc cttcagattc agacgagaca
      481 tgaaacttcc tctcttgacc acaagcagcg cgaacgacac ttcctgcagc ttctgcacag
      541 cctatgccat acgacgacac gcacccgata ttgatttgtg gtgacaggga gcggaaagca
      601 tcacgcgatc gtggtgatgg ctcgcgagcg agtcacaacg gcgccattgc cacacgcatc
      661 ttatgcaatc agtgcgccga tgtcagaaca tcttcccggc ggatcgtgaa tgatttcacc
      721 tctttccttg gagaaacctg aaaaccgacc agatgcacga aaagtgttat cgaggcggcg
      781 cattttcgaa gagctgggca gcttcccggc gcgatctcac gccgagtttg tcgaagatat
      841 tcgatacgtg gcgagatacg gttcgcgtgc tgatgaacag cgtctccgcg atctcgcgat
      901 cggtaaagcg ttgcagcaga agggccagga cctggtgctc gcgggaggtg agctcggcgg
      961 cgaccatcgg tcccgccaca ccgctcaccg cagatgcccc tggcgccccc gatgtcgtga
     1021 tgcgccccag ccaggcaacc gcctcgtcaa ccgcggcggc gaactcgaat ccacgaccac
     1081 gatcgaaggc gcgggcaaac gactcgctcc cgatcgcctg acgagcggat ttcgcggcaa
     1141 ccccgtagtg ctgttgctcc ggttgctcga gcgcgtagtc gatcgttcga cagagcgcct
     1201 ccacgcttcc gaagaatgtg gccgccagcc cgaacgcgcc cgccgcgacg gcgatcaccg
     1261 ccgtcgcggc gatcccgtcg atcaggcact cgcgcgattc gacggcacgc gccaggcgca
     1321 atccttccgt caccgcctcc gacgcggcgc gcggatcgcc ctgggcgcaa cgaatcaggc
     1381 cgagcgacat caacgtgaat gtgagccccc attcgttgcc ggccgcgcgg tacccacggg
     1441 ccgcggcctc gacgtacgct cgcgcttccc gcgtctttcc ccgccagaag gcgataacgc
     1501 cgagattgca gttgagcagg gccgtccacg atggatcgtc gtagttgccg aagattgcca
     1561 gcgactcctc gcagacctgt gtcgccgtca cgaagtcacc ctgtgcgcgg gagaccgccc
     1621 cgaggagatt gaggaccgcc gcctcccgca ttgggtcgcc gatcttccgg tagagggcga
     1681 ggctctggcg gcaccacgcc tcggccgcgc cgagatcgcc ttgcgtccgg gcgagcgccg
     1741 ccgacgcgag gtaggcgcca gcccggagat ccatcggcac atcctcgatc cccggctggg
     1801 aaagcgcccg tcgcaaccag gaactcccct cggcgcgaaa cgaccgcaat gtccagtacg
     1861 gggcgagttg cgccgtcatc cggagcaacg acgtggcgtc accttgccga tcgaaccatt
     1921 cgagcgcgcg ctgaagatcg ttgaactggg cgtcgatccg gcccagcacc aggggcggga
     1981 tgaaatcgac caccggcacc gaccagtagc cttcgaccag cctcatgcac cacgcggcgt
     2041 ggccggtgcg aagcgcatcc tcctcgcctt ccgccttcgc caggtcgcgg gcgaaggacc
     2101 ggatcgtcgc cagcatgaag aacgcgggct cgccacgctc tccctcgacc cggcgcagga
     2161 ggctcttctc caccagcgat tgcaccagct cgatcgcgtc gctcccggcg ctaccatccg
     2221 cccgcgcgca gatcgtttcg accgccgaga agggaaacgc catcgagaag acggaaagcc
     2281 agcgcagcat ccgctgctcg gatggggaga ggagacggta gctccactcg atcgtgtccc
     2341 gcatcgtctg ctggtgggcg agctggttgc ggggtccgcc ggcgagcacg tcgatgtcga
     2401 tgtggtcgcg cagacgctcg cggagcgctt ccggcgagag gaccttcatc cgcgcggccg
     2461 ccagttcgat cgcgagcgga agtccatcca gcaggcggca cacctccagg atcaccggag
     2521 ccgagcgacc ggtgagggca aagtcgggat cgaagcgctg cacgcgggcg acgaacagcg
     2581 cgaccgcctc cgactgtgcg agatcggcgg caaccggtgt gcgatccggc agttccaggc
     2641 agggcaggag gaattcgtgc tcgccgtcga cgtgcagggg ctcacggctc gtcgccagga
     2701 cgtcgatccg cgcggaaccg gcgatcaatt cggcgatgac ggaacccgca tcggaaatct
     2761 gctcgagatt gtcgaggatg agaagaacgc gctgttcgcg cgcgcggctc gcaatccgtt
     2821 gcacccagcc aacgttgggc tcatcaccgg cgccggcgcc acgcacgagc gtcgggggca
     2881 ggagcgcggg gtcgacgatc gaggcaagcg gaatgaaatg gatgcgttcg tagcgcccgg
     2941 tgagcccgtg cgcggcctcg agggcgaggc gggtcttccc gacgccaccg gggccggtga
     3001 ccgtcatcag ccggacatcg ggacgcgaga tgcgttcgac caacgcggcg aggatcgaga
     3061 cgcgaccgac gagcggatga agtggagcgg gaacatcgaa gacacgttcc gattgcggaa
     3121 agtgcaccac ggagatgcga ttcggggcca ccgggttggg cggagtggcg aggtcctgcc
     3181 catctgggaa ggatgacatc gtgagcctct ggctgattgc gagaatccgg ggacccggtg
     3241 gcggaatcgt cgtgtctttc cccgtctggg gctactgatc cctacggtga ctcaaacgca
     3301 cggacgatgc cgggggttcc gctcgcacag gggctctgat tattcagttg gcgcacacaa
     3361 ttgacaccgc acccctgggt gatcgcgacc atatgcccgc catacgcagg gccgcgcata
     3421 atgacggcat gattcccggt ggcggggtgg gaggtgacgc gagatgcggg ttctggttgt
     3481 cgatgatgaa gaggcgcttg cggatgccgt tgcccgtggc ctgcggcagg cgaatctcgc
     3541 cgtcgatatc gcgctcgacg gcgaatcggc cctgatgaag gccgaggtca cgccgtacga
     3601 cgtcgtcgtc ctcgatcgcc agctgccgga cgtgcacggt gacgacgtct gccgccaact
     3661 cgtcgccttc gagacgccgc cgcggatcct gatgctgacc gccgccggcg acgtccactc
     3721 gcggatcgag ggtctcgcga tcggcgccga tgactacctc ccgaaaccct tcgtgatggg
     3781 cgaactcatc gcccgcatcc gggcgcttca ccggcgtccc aacatcgcga tccgtcccgt
     3841 cctcacgatc ggcgatctcg tcgtcgacag ctcccgtcag gcggcgaccc gtgccgggcg
     3901 cgaactttcg ctcacccgca aggaattcgg ggtgctggaa gagatcgcgc gcgcgaacgg
     3961 cgcgatcgtc agttccgagg aactcctcga gcgcgtctgg gacgaggcgg cggatccctt
     4021 cacgaacgcc gtccgcatca cggtgatgac gctccgccgc aagctcggtg aaccggggat
     4081 gatcgagaca gtcgtcggct cgggatatcg cctgtgctcg caggaatagc cgctcgcctc
     4141 cacctgcgct ggaccgttcg cctccgcctg acgctcacct acacggcgct cttcgtcgtc
     4201 accggcgcct tgctgctggt gcttctctac gtcctgctga gcaatgccct ggcgccaccc
     4261 acgccccgta ccgggatccc ccagggctcg ggaccgatca ccaacatctc cagaggaaac
     4321 acggccgcag atcaggccga cgacaccctc aacgaccagc ttgacgagac gaggcgagag
     4381 gaacgctccg ccgccctcca gcaggtgcag gtccaggcca cgattgcgct cctgctgatg
     4441 agcgtcgcgt ccttcgtcct cggctggatc gtcgccggac gcgtgctgcg gccgatccgg
     4501 cagatcaccg cgcacgccca acacgcctcc gaggcgacgc tcaacgagcg catcgacctc
     4561 cgcgggccgc cagatgaact gacgaacctc gcgaacacga tcgacgggat gctcgatcgc
     4621 ctgcaatccg cgttcgagag ccagcgagac ttcgccgcga aggcctccca cgaactccgg
     4681 acaccgctgg cgatcatcgg cgccgaggcg gacatcgcgc tcgacgcgcc tgatgcctcc
     4741 gagcgcgagc ggacgaccgc gcttgccatc cgcaatgcga ccaaccgtag cgaacgcctg
     4801 atcgacggcc tgctgatgct cgcccgcagc gagagcacga tgcgggacaa cgcccggatc
     4861 gatcttgccg aggttgccgg agacatcgcg ggcgagcttg tcggcgaggc cgacatcgcc
     4921 ggtgtcgcga tcgatctcca gctcgattcc gccttcgtcc tcggcgaccg cacgctgctc
     4981 gagcggctgg tcggcaacct ggtcgagaac gggatccgct acaacatccg cggcggctgg
     5041 ttgcgtctcc acgtccggag ggacggcgat cgctccgtcg tcattgtcga gaacagcggc
     5101 aacgagcgcc cgatcggcaa tgtcaacgag ctcttcaagc ccttccagcg cggagggggg
     5161 gcaacatcca atcgtcgcga acgcacgacc ggcttcgggc ttggtctcgc catcgtccag
     5221 tccgtcgccg aggttcacgc ggcgacgatc gtcacgaccg cacgacagga cggcggcctg
     5281 cgcgtcgagg tccgcttccc cgccgcaccg gcccccgcgc gcctcccgac ccggaacccg
     5341 cagtggatct gagaccatac gcgaaccata cggacggctc cctaggatga cggtagtgga
     5401 tcgaaaccga aggcaacccg gatttcgagt cgccccgatc ctcatcgaac tggaggctcc
     5461 gtcatgacac tcgctcgcaa cgccaggact cgtcagatcg gaatcgcacc gcgccgttcg
     5521 ctcgcgatcg cccgcccgcc tctccccgtc gcacgacagc acgcgaccag gtcgcgatgg
     5581 agccgcctcg ttccccacca cgaagtgatc cccatgctcg cgacgatcac ggtcacgctg
     5641 ctgatcgcat gcgccatcat cgcgaacgcc gcgatgtcgc gcggcggctc gatcctgtcc
     5701 tgatcgtgat cgcccgaccg ctcccagcct tttcccagag gtgcccgaga aacttttcaa
     5761 ccaacgtcgt catcatgaac gcggcgaggc gagcaaccgg ttcgcggtcc tggtccatca
     5821 cagggcacgg aatcgggacc agacctcacc accaaccggg ccgttgggag gcaactcccg
     5881 gcggaatcag caggtcgcac ggcgcgctgt cggatcttcc cttgaaagtc cccgacgcat
     5941 cgcctggcgc acctattgtc ggcacaacca gccaagccga cgcgttaacc aatcgcggga
     6001 tcgtgacgca gggagagatt ccgggcggca cgctcctcac attgggggac ggcccgcagc
     6061 ctgcgacacg cagcgccgaa ggggccgccc ctcgcgccga gcgtcatcca ttcaagccga
     6121 tcaccccggc cctacctttg cgcttgggaa gcacgccgcg gcgagggaat ccatccggat
     6181 ctccccggtc gcggcaccag gtcgcatcgc ccactcacca gatcgacatc gatgccgcga
     6241 agagatcgcg atagtcatcc tcggacgacg gtcgcggtga aagctgcgtc aggcgcgtct
     6301 gcggcagtgt ccccttcacc agcgccggca tgtcatccag cgtgattccc acgccgttga
     6361 gcccgttcgg catctccacg cggtgcatca ggtcgatcac ggccccggcc agcagttcgc
     6421 cgccgtcctc cggcgccgct ccccgcgtgt cgacgcccag caaccgcgcc gcctcgagat
     6481 gcttcgccgg gttcgccgcc gctgtccatc ggaagatcgc gggcgcggtc aggatcaccg
     6541 agatcccatg cggcacgatc gcgtgatcgt ccggatagcc atc
//
LOCUS       DASTMD010000384         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3161619, whole genome shotgun sequence.
ACCESSION   DASTMD010000384 DASTMD010000000
VERSION     DASTMD010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3161619"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..908)
                     /locus_tag="VFQ54_05595"
     CDS             complement(219..908)
                     /locus_tag="VFQ54_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU0164500.1"
                     /translation="MSSPANPVVVPAQDPDERFDLVTAYGAPLGTSKRRADVHKEGDW
                     HRSIHVWIYGIDPETTEPFVLFQRRGLEKDTWPGMLDATAAGHLSSGETVPDAFREIE
                     EELGIAPDPSRLQHIGTRVCANEQPPTIFDRELQEVFFLREDGPLSLFHPNAAEVDAL
                     VRLPLGETLEFLGGFRGDVHGTVLFADDRREEGVTITAECFVPTMIDRYYYRVAIAIA
                     AVLRGDRYIAV"
     gene            1552..>1758
                     /locus_tag="VFQ54_05600"
     CDS             1552..>1758
                     /locus_tag="VFQ54_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164501.1"
                     /translation="MIVDSAIYVDGKRIVSPGTSFEKMYESCRSNGGIAWIGLYQPTP
                     DEFGRVAAEFNLHELAVEDAVQAHQ"
BASE COUNT          318 a          533 c          574 g          333 t
ORIGIN      
        1 agcgatgccg taggggcgag tctgtgctcg cccggtgcgc cagagggacg cgcggtgcgg
       61 gagcacgcgg atgccggctg atccctcgac aagctgctct gaaaaactcc gcccttcggg
      121 ctctccgacc caagcttttt catcccgcgt cggcgaggcg cgcttcatga ctactcggga
      181 ggacgtgtcg tgctgggaag gacgtgtgat gcggggggct agacggcgat gtagcgatcc
      241 ccgcgaagca cggcggcgat cgcgatcgcg acgcggtagt agtagcgatc gatcatcgtc
      301 ggtacgaagc actcggcggt gatcgtcacg ccctcctccc ggcgatcgtc ggcaaagagc
      361 acggtgccgt gcacgtcccc gcggaatccc ccgagaaact cgagtgtctc gccaagggga
      421 aggcgcacca gggcgtcgac ctccgccgca ttcggatgga agagcgacag cggaccatcc
      481 tcgcggagga agaagacttc ctgcagctcg cggtcgaaga tcgtcggcgg ctgctcgttg
      541 gcgcagacgc gcgtcccgat gtgctggagc cggctcggat cgggggcgat cccgagttcc
      601 tcctcgatct cgcgaaacgc gtcggggacc gtctcgccgg aggagaggtg acccgccgcg
      661 gtcgcatcga gcatccctgg ccaggtgtcc ttctcgaggc cgcgtcgctg gaagaggacg
      721 aacggctcgg tcgtctccgg gtcgatcccg tagatccaga cgtggatcga acgatgccag
      781 tctccttcct tgtggacatc cgcgcgtcgc ttgctcgttc cgagcggcgc gccgtaggct
      841 gtcacaagat cgaatcgctc gtcgggatcc tgggcgggaa cgacgacggg attggcaggg
      901 ctggacatgg gagaaggttc ctttacaacg tcgctgggaa cgcgaggcgg tgggtagcga
      961 atggacaaac ctcgatgttc gtcgcgcgat cgtgcattga taccacgcga tgcggggctt
     1021 tcgccgacat gacgaaacag agcgggatcg ggcagtggtt atgcccgtcc cgctctgttt
     1081 cagccttccc ctcccattac cgcctggcgg ccctgggctc cccggtagtt atcactgtga
     1141 tggcgtaaca tccgaccccc tggagtgtac gtacacccgt gtcagacatc gcgataacaa
     1201 tacaccatcg acctgtccgc gcacagggag attccgatga cgcctcatgc gggattgctt
     1261 cgccccttgc cgccgacatc ccgacggccc gggaatcaca tggcaggcgt catcttatgt
     1321 cattaatact atcccaatcc ccggacagtc tcccgatcgt tgacgccggg agtgtcgccg
     1381 ccgtaccgtg agttcgattt ctggtcgtga ccgtggcgcg aatgcaggct gatccattcc
     1441 cgggaatcgt tgaattcgca gtggtttcgc tccgccacga tgacttctca tccgcgggct
     1501 gtgacggcag gagtcgaatg tgtgcagagg gtcgggggag gaaggtgacg catgatcgtt
     1561 gacagcgcca tctacgtcga tggaaagaga atcgtttctc ccggcacatc cttcgagaag
     1621 atgtacgagt cgtgtcgcag caatggcggc atcgcatgga tcgggctcta ccaaccaacg
     1681 cccgatgagt tcggtcgtgt cgcggcggag ttcaatcttc acgaactcgc ggtcgaggat
     1741 gccgtgcagg cgcatcaa
//
LOCUS       DASTMD010000385         3809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2536365, whole genome shotgun sequence.
ACCESSION   DASTMD010000385 DASTMD010000000
VERSION     DASTMD010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2536365"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFQ54_05605"
     CDS             <1..653
                     /locus_tag="VFQ54_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164502.1"
                     /translation="LILSDRYLDDLGEGIYDTLSAIAPVIAPKEIEVSDAYALQQWEY
                     EQLIYGHAIGKLDASKQAILDLRARAAAIKARLGDQAGASVIAFRPRPEFAVIMSQQW
                     ITGNMLTWSGLAGTEFTESLAPPHSGNTVGIERLNLLDADWLFVAARNQDMVAELEVY
                     KQNPLFQTVPAYKSGQIALVSGDLWSGATGVLAGHAMLDDIERIIVDGDYIATPAA"
     gene            671..1948
                     /locus_tag="VFQ54_05610"
     CDS             671..1948
                     /locus_tag="VFQ54_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU0164503.1"
                     /translation="MTEIEQTIIESPTLQRLEVALAAGEPGAVETFWENVTASTTPLI
                     EPVEGEPDARLVTFLWRADGPTENVVHAEWFTAADFPEKQFSRIGETDVWYRTLKMRS
                     DVRVTYGIIPNDSLVKYEREKNWEARFAGMKDDPLNPQHVVGSHVVRREDTGLFDRNV
                     AVLPDAAPFVWSGRREEIPHGEVTHHRFASALLGNERDVWLYTPPVSGETTAARLLVH
                     FDGDRSIEVLDIPNLLDNLLSEGAIQPTVAVMIGNVDRGEELPCNAAFASFIADEVIP
                     WVRGQVAIAEGPQAVVAAGQSYGGLAATWCGLTRPDAIGNILSQSGSFWWKPDPRNDK
                     APIISGDAPEYGWLPQQVATWETIPVRIYMEAGRFEARNSGVMPSLLSTNRHMRDVLI
                     AKGYDVTYSEYAGGHDYVWWKELLAPGLIHLAG"
     gene            complement(2294..3523)
                     /locus_tag="VFQ54_05615"
     CDS             complement(2294..3523)
                     /locus_tag="VFQ54_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164504.1"
                     /translation="MTEQSKPAQPGISTGLVLILAVATGLSVANLYYAQPLLHTISNS
                     LHVGEGASGLVVTFTQIGYALGLALLVPLGDIVQRRKLTSILLILDAIALVGSAIAPN
                     LGVLIGVAAFVGIGSVSVQILIPLAANLATDEKRGQVVGMVMTGVLLGILLARTISGV
                     IAGATSWRVVYWAAAAIALILAAVLFRVLPEEIRPPGARIGYSSLLRSTLALVRDEPV
                     LRQRMLMGSFSFAAFSALWTTIGFLLAGDPYNYGTTLIGLFGLVGVAGALSGNFAGRF
                     VDRGLQSVTTIVFASGTTISFLLIYFGKTSLAMLIVGILLLDIGIQGLQITNQSVIYQ
                     LAPHARGRITANYVTSNFIGGAIGSAAASAIFDSSLRWGGVCLFGGLLGVGILVTYVV
                     SRVRAGTPARRGISPAR"
BASE COUNT          742 a         1214 c         1254 g          599 t
ORIGIN      
        1 atctgatcct ctctgaccgc tatctcgacg acctcggcga gggtatctac gacacgctca
       61 gcgcgatcgc gcccgtcatc gcaccgaagg agatcgaggt ttccgatgcc tacgccctgc
      121 agcaatggga atatgagcag ctgatctacg ggcatgcgat cggaaagctg gacgcgtcga
      181 aacaggcgat tctcgacctg cgggcacggg ccgccgcgat caaggcgcgt ctcggtgacc
      241 aggcgggagc gagcgtgatc gccttccgcc cccggccgga attcgccgtg atcatgtccc
      301 agcagtggat caccggcaac atgctcacct ggtccggtct cgccggcacg gagttcacgg
      361 aatcgctcgc cccaccgcac agcggcaata cggtcgggat cgagcggctg aacctgctcg
      421 atgccgactg gctcttcgtc gccgcgcgca accaggacat ggtggccgaa ctcgaggtct
      481 acaagcagaa tccgctcttc cagacagttc cggcgtacaa gtcggggcag attgccttgg
      541 tctccggcga tctctggagt ggtgcaaccg gggttctcgc cgggcacgcg atgctggatg
      601 atatcgagcg catcatcgtc gacggcgact acatcgccac tcccgccgcg tgaggagacc
      661 gagcattccc atgaccgaga tcgaacagac catcatcgag agcccgaccc tgcagcgact
      721 cgaggtcgcg ctcgccgccg gggagcccgg cgccgtcgag accttctggg aaaacgtcac
      781 cgcgagcacg acgccactga tcgagccggt cgagggcgag ccggatgcga ggctcgtcac
      841 cttcctctgg cgcgccgacg gtccgaccga gaacgtcgtc catgccgagt ggtttacggc
      901 ggcggacttc ccggagaagc aattcagccg gatcggcgag acggatgtct ggtaccggac
      961 gctgaagatg cgctccgacg tgcgggtgac ctacgggatc atcccgaacg actcgctggt
     1021 gaagtacgag agggaaaaga actgggaagc ccgcttcgcc gggatgaagg acgatcccct
     1081 caatccccag catgtggtcg gcagtcatgt cgtgcggcgt gaagataccg gacttttcga
     1141 tcgcaacgtc gcagtgctgc cggatgcggc cccgttcgtc tggtccggac gacgagagga
     1201 gattccgcac ggcgaggtga cgcaccatcg cttcgcgagc gcgctgctcg gaaacgaacg
     1261 tgacgtctgg ctctacaccc cgccggtgag cggggaaaca accgcggccc ggctgctcgt
     1321 tcatttcgac ggcgaccggt cgatcgaggt gctcgacatt ccgaacctgc tcgacaacct
     1381 gctttcggaa ggagcgattc aaccgacggt cgccgtgatg atcgggaatg tcgatcgcgg
     1441 cgaggaactt ccctgcaatg cggccttcgc gagcttcatc gccgacgagg tgatcccgtg
     1501 ggtgcgcggt caggtcgcaa tcgcggaggg cccgcaggcc gtggtcgccg cgggtcagag
     1561 ctacggcgga ctggcggcga cctggtgtgg actgacgcga ccggatgcga ttgggaatat
     1621 cctctcgcag tcgggttcgt tctggtggaa gccggatccg cgaaacgaca aggcgccgat
     1681 catcagcggc gatgcaccgg aatatgggtg gctcccgcaa caggtcgcga cctgggagac
     1741 gatccccgtc cgcatctaca tggaggctgg tcgcttcgag gcacggaaca gcggcgtgat
     1801 gccgagtctc ctctcgacga accggcacat gcgcgatgtc ctgatcgcga agggctacga
     1861 cgtcacctac agcgaatacg ccggcggtca cgactacgtc tggtggaagg aattgctggc
     1921 gcccggactg atccatctcg ccggctagag caagggtccg aagacgtgcc tttcgctcca
     1981 actcctggcc gtcatcctga gcttgtcgaa ggatccccgc gcgaagcgca tccggcgcag
     2041 ccggactcca ctgcggtcgt ttggcctgcc gcgtcgccgg ccataggtct ccgcagtccc
     2101 gcccgacgaa cgcgacgctg caccaaagaa gtcgtcagga gcatgacggt cacgaaacgc
     2161 ggtcgttcgt ggggcgggac tgcgcaagcc aatgatcggc atgagcgata tcttgcctgg
     2221 atggagtccg gtcgttggct ggaacaatgt cccggctccc gatgcgaaca acaggttccc
     2281 gtcccccgat gcgctaccgg gccggtgaga tcccgcggcg cgccggagtg ccagcgcgca
     2341 cccgggacac gacgtacgtc acgagtatcc ccaccccgag caggccgccg aagaggcaga
     2401 cgccgcccca ccgtagcgat gaatcgaaga tcgcactcgc cgcggccgat ccgatcgcgc
     2461 cgccgatgaa gttcgatgtc acgtagttcg ccgtgatccg gccacgggcg tggggcgcaa
     2521 gctggtagat cacgctctgg ttcgtgatct gcaacccctg aatcccgatg tcgaggagga
     2581 ggatgccgac gatcagcatc gccagtgagg tcttgccgaa gtagatcagc aggaaggata
     2641 tcgtcgtccc gctcgcgaag acgatggtcg tcaccgactg caagccacga tcgacgaagc
     2701 gtccggcgaa gttgccgctg agggcgccgg cgaccccgac gaggccaaag aggccgatca
     2761 gggtcgtgcc gtagttgtag ggatcaccgg cgaggaggaa gccgatcgtc gtccagagcg
     2821 cactgaaggc ggcgaagctg aaggagccca tcagcatgcg ctggcggagg accggttcgt
     2881 cgcgaacgag ggccaaggtc gagcggagca gggacgagta gccgatccgg gcgcccggtg
     2941 gccggatctc ctccgggagc acccggaaga gcacggctgc gaggatgagg gcgatcgccg
     3001 cggccgccca atagacgacg cgccagctcg tcgccccggc gatgacgccc gagatcgtcc
     3061 gcgcgagcag gatcccgagc aggacgccgg tcatcaccat gccaaccacc tgaccgcgct
     3121 tttcgtcggt cgcaagattg gcggccagcg gaatcaggat ctggaccgag accgatccga
     3181 taccgacgaa agcggcgacg ccgatcagga cgccgagatt cggcgcgatc gctgaaccga
     3241 cgagcgcgat cgcatcgagg atgagcagga tcgatgtgag cttgcgtcgt tggacgatat
     3301 cgccgagcgg aaccagcagc gcgagaccga gcgcatagcc gatctgggtg aaggtgacga
     3361 caaggccgga tgcgccttca ccaacgtgga gcgagttcga gatcgtgtgg aggagcggct
     3421 gcgcgtagta gagattcgcg acggaaagac ccgtcgcgac ggcgaggatc aggaccagcc
     3481 cggtgctgat ccccggctgc gccggcttcg attgttcggt catggaagac gtggcccctg
     3541 gatgtggaca agaatccgac gcgatgtccg ttacccggga cacttacgaa aagtatacgt
     3601 cccggcctgc gggatcggcg tcccgcccca cattccttgc gtacaatgct gtcccagaca
     3661 cgtcctccaa gcccgtcgaa gaccgctcag gcatcacgcg ccctgcatac aacgccaccc
     3721 cattcccgtc ctcccgagcc tgtcgaggga ccagttggca tccacgtgct ccccgaacaa
     3781 cagcggctgc caggcggccc gtcgcgcaa
//
LOCUS       DASTMD010000386         1829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_394097, whole genome shotgun sequence.
ACCESSION   DASTMD010000386 DASTMD010000000
VERSION     DASTMD010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394097"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..755)
                     /locus_tag="VFQ54_05620"
     CDS             complement(96..755)
                     /locus_tag="VFQ54_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU0164505.1"
                     /translation="MYIIVVGGGKVGYYLTKTLLNEGHEVLLIERDAQKVDTYSERFG
                     SVVLAGDGAEAATLASAGAARADVVIAVTGEDEDNLVVCQVAKSKFHVGRTIARVNNP
                     KNEQLFRLLGIDVTVSQTNYILNLIEQAIPERSFVHLLSLRHADLAIIEATIGPNSPV
                     VNQAIDEIELPVDCVIAAIARGTELIVPRPSTEILAGDEVIAITHRAEEDELRRLLIP
                     D"
     gene            complement(776..1195)
                     /locus_tag="VFQ54_05625"
     CDS             complement(776..1195)
                     /locus_tag="VFQ54_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU0164506.1"
                     /translation="MNVVIMGCGRVGARVASLLDHNGHNVTVIDTHSSAFKRLSTEYR
                     GDTLIGTGIDEDVLRAAGIEEAHAFIAVTNGDNRNIMAAQVARAIFNVPEVIVRIYDP
                     VREDTYRRLGLTTVCPTTTVSALILDHVISAGDGFRS"
     gene            complement(1192..1710)
                     /locus_tag="VFQ54_05630"
     CDS             complement(1192..1710)
                     /locus_tag="VFQ54_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0164507.1"
                     /translation="MVSLRQAFRPVQSDRNIPISRRILVPVSGAESDQRLLTLVEKIA
                     RKQNAFVTIVYVVEVQQSMPLDAELPAEIERGEQVLRDAEKLGLTCVDGKTSMLSTEL
                     LQARSAGAAIVDEAIDRNADVIMMGATIRKKYGKATIGETVDYVLKNAPCEVFIIRPA
                     MPDWVTEGLEHE"
BASE COUNT          313 a          559 c          585 g          372 t
ORIGIN      
        1 cgccgctgcc ggaccgaagg tccggacggc ctgtcccgcc tcacgttcgt ccgaaggccg
       61 tgcttcccgg tggtagccgc gtcgaatcgc cgggcctagt ccgggatcaa caggcggcga
      121 agctcgtcct cttccgcgcg atgggttatc gcgatgactt cgtcaccggc caggatctcg
      181 gtcgaaggtc gcggaacgat gagttcggtg ccgcgcgcga tcgccgcgat cacgcagtcg
      241 accgggagtt cgatctcatc gatcgcctga ttcacgaccg gggaattcgg cccgatcgtc
      301 gcctcgatga tcgcgagatc ggcgtggcgg aggctaagga ggtgaacgaa gcttcgctcc
      361 gggatcgcct gttcgatcag gttgaggatg tagttcgtct ggctgactgt cacgtcgatc
      421 ccgagcaggc ggaagagctg ctcgttcttg ggattgttga cgcgggcgat cgtgcgcccg
      481 acgtggaact tcgacttcgc gacctggcag acgacgagat tgtcctcgtc ctcgccggtc
      541 acggcgatca cgacgtccgc gcgggcggcg ccggccgaag ccagtgtcgc cgcctcggcg
      601 ccgtctcccg cgaggacgac cgaaccgaat cgctctgaat aggtgtcgac cttctgggcg
      661 tcccgctcga tgagaaggac ctcgtgaccc tcgttgagga gggtcttggt gaggtagtag
      721 ccgactttgc cgccgccgac gacgatgatg tacacgggga ttcttccttc gtctctcagg
      781 atcggaagcc gtcaccggcc gagatcacgt gatcgaggat caacgcggag actgtcgtgg
      841 tcgggcagac ggtggtcagg ccgagtcgac ggtaggtatc ctcgcgcacc ggatcgtaga
      901 tacggacgat gacttccgga acgttgaaga tcgctcgtgc cacctgcgcc gccatgatgt
      961 tgcgattgtc gccattcgtc acggcgatga acgcgtgggc ttcctcgata cctgccgcgc
     1021 gcagcacatc ctcgtcgatt cccgtgccga tcagggtgtc accccggtac tcggtcgaga
     1081 gtcgcttgaa cgcggatgaa tgggtatcga tgacggtgac gttgtgtccg ttgtgatcga
     1141 ggagcgaggc gacgcgggcg ccaacgcgcc cgcatcccat gatgaccacg ttcattcgtg
     1201 ttccagaccc tcggtcaccc aatccggcat cgccggacgg atgatgaaca cctcgcaggg
     1261 tgcgttcttc agcacgtagt cgacggtctc gccgatggtc gccttgccat acttcttgcg
     1321 gatggtggcg cccatcatga tgacgtcggc gttgcgatcg atcgcctcgt cgacgatcgc
     1381 cgccccggcg gaacgggcct gcaggagctc ggtcgagagc atcgacgtct tgccgtcgac
     1441 gcaggtgagg ccgagcttct cggcatcacg cagcacctgt tccccccgct cgatctcggc
     1501 cggcaattcg gcgtcgagcg gcatggattg ctgaacttcg acgacgtaga cgatcgtcac
     1561 gaaggcgttc tgctttcgtg cgatcttctc gaccagtgtc agcaggcgct ggtcgctttc
     1621 ggctccggaa acggggacga ggattcgccg ggagatgggg atatttcgat ctgattgaac
     1681 tggtcgaaat gcttgacgca gactgaccaa ggactttaat ccttcgcttt taccactcgc
     1741 agaacgtctg gagcttcgcg cactcgcgca accaagaatt atacgacatt tcccgcggct
     1801 caacgagcgc cattgtactc cttcgcggc
//
LOCUS       DASTMD010000387         2922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1823623, whole genome shotgun sequence.
ACCESSION   DASTMD010000387 DASTMD010000000
VERSION     DASTMD010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823623"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            648..1082
                     /locus_tag="VFQ54_05635"
     CDS             648..1082
                     /locus_tag="VFQ54_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164508.1"
                     /translation="MADKLRDDVNHQPQPTASNEENQPEEVSGRLVGIGSDISLTTAA
                     RRPARSLGRRHERYIRSTPPIYRWVCPCQTPPILLATYGPNGRINIKVRDRYWHLQGF
                     GMVEAICPRCAAEHMLDMRLLRNALDNVPPPEDDDIVANTAD"
     gene            1204..2091
                     /locus_tag="VFQ54_05640"
     CDS             1204..2091
                     /locus_tag="VFQ54_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0164509.1"
                     /translation="MTHQSRVLRSLRARRASESSRAQAAAVSGRTRITHINPIPANKA
                     GTTTIGAHGGLIAIELAVVSAGGVAAVSFRNPLAPIVPERVLLPVIGVNDELLPAFER
                     TLEPIVRRAWSGDRAARDALYAAFEPKLIRFSRRIHVPFAPTGSTTTWDRGDVAQEVY
                     LAFVDVISTWTPPIPFGRYVLAHFPWRLRDFVYRGVARTGIPPSGTVVAMDRIDRLGD
                     DSASAAESRVLLESIAASFEAPYDDILRWHIRDGESLMTIAGRLGCSRRTATRRWRVI
                     LDRLRVEFDEMTVSDLGRS"
     gene            complement(2588..>2922)
                     /locus_tag="VFQ54_05645"
     CDS             complement(2588..>2922)
                     /locus_tag="VFQ54_05645"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0164510.1"
                     /translation="ASWCGPCQEEMPAIQAISTSAPADVVFVGVGAKNDKDADAEGFV
                     QKYGITYDVGRDTVGGNNLSGAIERAFGIPGYPSTIVIDADGNVVGTQIGPISTDQLN
                     AYIAAARG"
BASE COUNT          484 a          964 c          944 g          530 t
ORIGIN      
        1 gcgaccacgt gggtcgccgg gacgggcgcg gcatgcccgg tgtggacggt tgcgatcagc
       61 cggggtctgt agggcaagac cttgtgtctt gccgcatgcc caacgtccca ttcatcgccc
      121 cccgccctcg cggtaacgcc ccattcatcg cccaacgtcc ccatttatcg ccccccccgc
      181 cgccctcgcg gtaacgtccc atgcaacgcc ctcgcggcaa cgcgcatcca tcagccgatc
      241 ggcggagttt atcctgagcc tgccgcatgg accgatccga aacggggaca agctccgcgg
      301 ggacggtgca tgcaagccat gacagcccgg ctcaacattc ctccttctgc cgttctcccc
      361 tcgttccttg gttgacagtg cccgtcttca tccctaaaat acgaacaaga aatagaacag
      421 atgttctatt tgaggcgtgg caggcgggtg cggtcgtgcg tgtcagcagt ttgaccgtgg
      481 cgccatgagt caccaataac caggatcgcg ggcacggtcg ggcgcgagga tggaacgtcg
      541 cggtcgacgg acgtctccgc gttccgggtc gtatcaggtc gatgctccgg atatgcagat
      601 caagccgacg tgcttgctgg caaggaatat cggaagggaa aagaggaatg gcggataagc
      661 tccgggacga tgtgaatcac cagccacaac cgaccgcgtc caatgaggaa aaccagccgg
      721 aagaggtgtc cgggcgtctc gtcggaatcg gatccgacat ctccctcacg accgccgccc
      781 gtcgtcccgc acgatcgctc ggccggcggc acgaacggta cattcgctcc acgccaccca
      841 tctatcgctg ggtctgcccg tgtcagactc cgcccatcct gctggcgacc tacgggccga
      901 acggcaggat caacatcaag gtgcgcgatc gctactggca cctgcaggga ttcggaatgg
      961 tcgaggcgat ctgcccccgc tgcgccgccg agcacatgct cgatatgcgg ttgctccgaa
     1021 acgcgctcga caacgtgccg ccgccggagg atgacgacat cgtcgccaat accgccgact
     1081 gatccgtccc gaagaacccg cgaatacgcc cacgacgagc tcagaagagc ctttcccgta
     1141 gttctcgaag atgcgcggcc cttccccggg gaggggatcg cgaaaacgac ggaggctctt
     1201 cccatgactc atcaatctcg cgtcctgcgc tcgctgcgtg cccggcgtgc atccgaatcg
     1261 tcccgcgcgc aggcggctgc cgtctccggc aggacccgca tcacccacat caatcccatc
     1321 ccggcgaaca aggcaggaac aaccacgatc ggggcgcatg gcggcctgat cgcgatcgag
     1381 ctcgcggtgg tctccgccgg cggggtggcc gcggtttcct tccggaaccc gctggcgccg
     1441 atcgtgccgg agcgggtgct gctcccggtg atcggggtga acgacgagct gctgccggca
     1501 ttcgaacgga cgctcgagcc gatcgttcgc cgcgcctggt ccggcgatcg cgccgcgcgg
     1561 gatgcgctct atgccgcctt cgagccgaag ctgatccgct tctcccgtcg catccacgtt
     1621 cccttcgcac cgacaggatc gaccacgacc tgggatcgcg gggatgtcgc ccaggaggtg
     1681 tacctcgcct tcgtcgatgt catctcgacg tggacgccgc cgatcccgtt cgggcgctac
     1741 gtgctcgctc atttcccgtg gcgattgcgt gatttcgtct accggggagt tgcgcggacc
     1801 gggattccgc cgtccggaac cgtggtcgcg atggatcgta tcgatcggct tggcgatgat
     1861 tcggcgagtg cggcggagtc gcgggtgctc ctcgaatcga tcgcggcgtc gttcgaggcg
     1921 ccgtacgacg atatcctgcg ctggcatatc cgtgacggcg aatcgttgat gacgatcgcc
     1981 ggacggctgg ggtgcagtcg gcggacggcg acgcgtcgct ggcgtgtgat cctcgatcga
     2041 ctccgtgtgg agttcgacga gatgacggtg tccgatctcg gcagatcgtg agggagtacg
     2101 ggcatgtcag gcgtcgcctg acggatggac attgaagtcc atccctacgt acgaccgctg
     2161 tgtggacgat cgtgccggcg acggatgtcg cggtcggcgg tcgtgcctgg ggtggcattg
     2221 gagggaggaa tgtcccgccc ctgcgtcccg aatgccagat ctttgggatc gatgcgctgc
     2281 caggtcacac gaggtgacct gctacggcag cagtggcgga gtggcggtcg gtccggtcgt
     2341 tcgtagggcg ggaccgccgt tgcccgaccg gaggtccgga cggcctgtcc cgcctgccct
     2401 ctccgagaac cgtgctcccc gtcgcgacga tcccggattt cgctctcgtg gtgcgtcacc
     2461 ggtgttgtgc cggttggcat cgtcagatgc ggcttcgccg catgggccac acccttcggc
     2521 gacctcaggg caggctcgtt ggtggcccgc tacggaaccg tcgtggcaat tcaggcgtga
     2581 cgcgccacta accgcgggcg gcggcgatgt aggcgttgag ctgatcggtc gagatcggcc
     2641 cgatctgggt gccgacgaca ttgccgtccg cgtcgatcac gatcgtcgac ggataccccg
     2701 gaatcccgaa cgcgcgctcg atcgcgccgg agagattgtt gccgccgacc gtatcgcgac
     2761 cgacatcata ggtgatgccg tatttctgga cgaagccctc ggcatcggca tccttgtcgt
     2821 tcttggcgcc aactccgacg aagaccacgt cggcaggcgc cgacgtcgaa atcgcctgga
     2881 tcgcgggcat ctcctcctgg cacggtccgc accaggacgc cc
//
LOCUS       DASTMD010000388         4268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2804447, whole genome shotgun sequence.
ACCESSION   DASTMD010000388 DASTMD010000000
VERSION     DASTMD010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2804447"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..947)
                     /locus_tag="VFQ54_05650"
     CDS             complement(387..947)
                     /locus_tag="VFQ54_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0164511.1"
                     /translation="MTSPDGGEQDAHDAAVRADLAGQLVEVIPAFSRWIGSIRNAETP
                     FGRVAYRQVEILYLIRKQYVPTERLSPSYLADRFSVQRSVVTRLLASLEANGFIERRV
                     DPDDGRGYFIDITDKGRAVSEYIEAIFTSEMREGIATLQRDQVAALTAQVEALRQLAR
                     ELDEKRKERLDRLHGDGYRSFDDEPL"
     gene            complement(1061..1759)
                     /locus_tag="VFQ54_05655"
     CDS             complement(1061..1759)
                     /locus_tag="VFQ54_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164512.1"
                     /translation="MSESPAQPAARPLSQLTYTRIQEHVDRAETLELITLHGHNQYGR
                     DLAGFARAAAPEGHLLGLESYKGVFVGRDIVGYTWFPGPLDRPAPVFFGDALAEIERF
                     LWDERGRQIARGAPVKPFLLGVEQGAIMALAAALTVPDLLSGVIAIGDFFPIVPGWDP
                     PLAPLDGLPILLLESGAMDASSGANVLTGQPLDAQLAAWDATVTRKRIVAGEIPAREM
                     SSWIAGQPVREFGD"
     gene            complement(1828..3669)
                     /locus_tag="VFQ54_05660"
     CDS             complement(1828..3669)
                     /locus_tag="VFQ54_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU0164513.1"
                     /translation="MAESTQGRLRVSRRGFLKASGAGAAVAAGTIGAVSFGSSRAFAQ
                     QKWDDTYDVVVVGSGAAAFSAAITAQHLGAKVVMLEKGAYVGGTTLVSGGTMWIPNNA
                     QMAAAGLKDNREDAIRYMARVAFPADYNPEDAKLGLMDHDFEMLSAYYDTGSEAMKTL
                     EDAGAATYLTSINTLTGSIQADYVDHLPEDTSPIGRSIVAATKDGKPGGGGDLIASYQ
                     AYAKKVGIPILLGHRVERVVLNDAGEVIGVEVAVNQAVASGTPEAAGAATPVATPAPT
                     QTIAIRANKGVIFGSGGFVRNKDLMRHFVSHPTYGGCSAPTNEGDLIRIAYSVQAKLG
                     NLHNIWRNEGLFEQAIASEFAYNCIWFYNGDSFIMVNKEGKRFVNEKRNYQDRSMSHL
                     TWDSNNATWANLLSFLIYDNRIQENWGTGFPFPADATTSPIVVIGETLEELATKLHDR
                     VDSLTSHTGGVQLGDDFAANLVAEVEKYNGYAKTGIDPDFERGKFLYDQDTPLPPFTA
                     KPTVTKWPSDDQPNSAMYPLSATGPYYALVVAQSAVDTNGGPVINSNGQILNIDEQPI
                     AGLYGAGNCVASPGVNAYWGAGMTLGNAHTWGYAAAKHVVASPEKSA"
     gene            3922..>4268
                     /locus_tag="VFQ54_05665"
     CDS             3922..>4268
                     /locus_tag="VFQ54_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0164514.1"
                     /translation="MSETRTAVVTGASSGIGEATVRLLRSHGWDVVGVARRADRLARL
                     ADETGAATFVADVTSDADVEALVTFLRERGQVNALVNVAGGAIGADPIEHAPLDAWRQ
                     MYETNVLGVVRVT"
BASE COUNT          702 a         1375 c         1443 g          748 t
ORIGIN      
        1 ccgacgtggg gtgtcaggga gggtattcgg gcatagccgt tgagaccgcg gtcgttcgta
       61 gggcgggacc gccgctgccg gaccgcagtc cggacggcct gtcccgcctc cctccacggc
      121 acgatgctct tgtggccgca cgactcgggg aacatcgcgc gaggaaggtt cccgttggag
      181 cccggcgtcg ccgggtgcgc ttcgcgcggg gatgtctcga caggctcgac atgacaattc
      241 tggggtgacg actcggggcg ggaccgccgg cgactcctta tgcgatgcgg tcgttcgtag
      301 ggcgggaccg ccgctgccgg accgcaggtc cggacggcct gtcccgcctc ccctcccccg
      361 gcacgatgtg gagtgtcccg ccccgtctac agcggctcgt cgtcgaacga gcggtatcca
      421 tcgccatgca ggcgatcgag ccgctccttg cgcttctcgt cgagttcgcg cgcgagctgc
      481 cgcagggctt cgacctgcgc ggtgagcgcc gcgacctgat cgcgctgcaa cgtcgcgatg
      541 ccttcccgca tctcgctggt gaagatcgcc tcgatgtact cgctcaccgc acgacccttg
      601 tcggtgatgt cgatgaaata gccgcggcca tcgtcgggat cgacgcgtcg ctcgatgaag
      661 ccgttcgcct cgagactcgc cagcaaccgc gtgacgacgc tgcgttgcac ggagaagcga
      721 tccgcgagat aggacggcga gaggcgctcg gtcgggacgt actgtttgcg gatcagatag
      781 aggatctcga cctggcgata ggcaacgcgg ccaaacggcg tctccgcatt gcggatcgag
      841 ccgatccagc gcgagaaggc ggggatgacc tcgacaagtt gaccggcgag gtcggcgcgc
      901 accgccgcgt catgggcatc ctgttccccg ccgtccggac tcgtcatcga tcccgctctc
      961 ctcgctgcaa ccggcgtgcg ccggccgcga agacgtgccg ccgggaaggt caacttgcag
     1021 gatcgcgcat ttgcggagcg ggtaccaggg tcaatgcgga tcagtcgccg aactcgcgca
     1081 cgggttgtcc cgcgatccag gacgacatct cgcgagccgg gatctcgccg gcgacgatgc
     1141 gtttccgcgt cacggtcgca tcccaggcgg caagttgcgc gtcgagcggc tggccggtca
     1201 gcacattcgc accggatgac gcatccatcg cgcccgattc gaggaggagg atcggcaggc
     1261 cgtcgagcgg cgcgagcggc gggtcccacc ccggaacgat cggaaagaag tccccgatcg
     1321 cgatgacgcc ggagagcaga tcggggaccg tcagcgcggc ggccagcgcc atgatcgccc
     1381 cctgttcgac cccgaggagg aatggcttga ccggcgcgcc acgcgcgatc tggcgcccgc
     1441 gctcgtccca gaggaaccgc tcgatctcgg cgagcgcgtc accgaagaag acgggggcag
     1501 ggcggtcgag tggaccggga aaccaggtgt agccgacgat atcccgaccg acgaagacgc
     1561 ccttgtagga ctccagcccg aggaggtgtc cctccggtgc cgccgcccgc gcgaagccgg
     1621 cgagatcgcg gccgtactgg ttgtggccgt gaagcgtgat cagttccagc gtctccgccc
     1681 gatccacgtg ctcctgaatc cgggtgtagg tgagctgact caacgggcga gcggcgggtt
     1741 gtgccggtga ttcggacatg acgatcctca tgacgaacgg gagggagctg cgttcagccc
     1801 cctcccgtca tgctatcgca acgagcgtca ggccgacttc tcgggcgatg cgacgacgtg
     1861 cttcgcggcg gcataccccc acgtatgcgc gttgccgagc gtcatgcccg caccccagta
     1921 ggcgttgaca ccggggctgg cgacgcagtt gccggcgccg tagagcccgg cgatcggctg
     1981 ctcgtcgata ttgaggatct gcccgttcga attgatgacc gggccaccat tcgtatcgac
     2041 cgcggactgc gcgacgacca gggcgtagta gggtccggtc gcgctcagcg ggtacatcgc
     2101 gctgttcggc tgatcgtcgg acggccactt cgtgacggtc ggcttcgcgg tgaacggcgg
     2161 gagcggcgtg tcctggtcgt agaggaactt gccacgctcg aagtcgggat cgatgcccgt
     2221 cttcgcgtag ccgttgtact tctcgacctc cgcgacaaga ttcgcggcga aatcgtcccc
     2281 gagctgaacg ccgcccgtgt gcgacgtcag gctgtcgacg cgatcgtgga gcttggtcgc
     2341 gagctcctcc agcgtctccc cgatgacgac gatcggcgag gtggtcgcat ccgccgggaa
     2401 ggggaagccg gtgccccagt tttcctggat gcggttgtcg tagatcagga agctgagcag
     2461 attcgcccag gtcgcgttgt tggaatccca ggtgagatgc gacatcgagc ggtcctggta
     2521 gttgcgcttc tcgttgacga agcgcttgcc ttccttgttg accatgatga aggagtcacc
     2581 gttgtagaac cagatgcagt tgtaggcgaa ctcgctggcg atcgcctgct cgaagaggcc
     2641 ttcgttgcgc cagatgttgt ggaggttgcc gagcttcgcc tgcaccgaat aggcgatccg
     2701 gatcaggtcc ccctcgttgg tcggggcgga gcatccaccg taggtgggat ggctcacgaa
     2761 gtggcgcatc agatccttgt tgcgcacgaa gccaccgcta ccgaagatga cacccttgtt
     2821 ggcacggatc gcgatcgtct gggtcggcgc gggggttgcg actggtgtcg ccgcaccggc
     2881 agcctccggt gtacccgacg ccacggcctg gttgaccgca acctcgaccc cgatcacctc
     2941 accggcgtcg ttgaggacga cgcgctcgac gcggtgaccg agcaggatcg gaatcccgac
     3001 cttcttggcg tatgcctggt agctcgcgat gagatcgcca ccgccacccg gcttcccatc
     3061 cttggttgcc gccacgatcg agcggccgat cggcgaggtg tcttccggca gatggtcgac
     3121 atagtcggcc tggatcgagc cggtcagcgt gttgatcgag gtgaggtagg ttgccgcacc
     3181 ggcgtcttcg agcgtcttca tcgcctcgct gccggtgtcg tagtaggcgg agagcatctc
     3241 gaagtcgtgg tccatcagac cgagcttcgc gtcttccggg ttgtaatcag ccgggaaggc
     3301 gacgcgcgcc atatagcgga tggcatcctc acggttgtcc ttgagtcctg ccgctgccat
     3361 ctgcgcgttg ttgggaatcc acatcgtgcc acccgaaacg agggtggtgc cgccgacgta
     3421 ggcgcccttc tcgagcatca cgaccttggc gccgagatgc tgcgccgtga tcgcggcgga
     3481 gaaagcggcg gcaccgctgc cgaccaccac gacgtcgtac gtgtcgtccc acttctgctg
     3541 ggcgaacgct cgcgatgaac caaacgaaac cgcaccgatc gttccggccg caaccgcggc
     3601 tccggcgccg cttgccttga gaaatccgcg ccgggagacg cgaagtcgcc cctgtgtcga
     3661 ctctgccaca gttatcaccc tcttctgtac atctgcgtac gaacggcatg gtcccacccg
     3721 ccatgggggg acgtttgaaa ggacccgatg gctgcataat acgcaatgac aactatctct
     3781 acaaggtggg gaagcatcca atttcgtggc gctttcgcgt acgattccgg ggcttcgccc
     3841 gtatcgcgga caggcgtggt accgggagcg tcgcaggcat cgacgccatc atcaagttgc
     3901 aagcccaggg ggagaacgta gatgtcagaa acacgaacag cggtggtgac cggagccagt
     3961 tccgggatcg gtgaggcgac ggtgcggctg ctccggtcgc acgggtggga tgtcgtcggt
     4021 gtcgcccggc gcgccgaccg cctcgcgcgc ctcgcggacg agaccggcgc cgcgaccttc
     4081 gtcgccgacg tcacgagcga tgcggatgtc gaggcgctcg tgaccttcct gcgtgaacgc
     4141 ggccaggtga acgcgctcgt caacgtcgcc ggcggcgcga tcggcgccga tcccatcgaa
     4201 catgcgccgc tcgacgcctg gcgccagatg tacgagacga acgtcctcgg cgtcgtccgc
     4261 gtcaccaa
//
LOCUS       DASTMD010000389         1812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_749783, whole genome shotgun sequence.
ACCESSION   DASTMD010000389 DASTMD010000000
VERSION     DASTMD010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749783"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VFQ54_05670"
     CDS             complement(<1..865)
                     /locus_tag="VFQ54_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164515.1"
                     /translation="MNSNHLSKAAPASAPLPTFEQFTAIRRFQGTLAFSPDASEVAYS
                     VNTSGQFNLWRQSSDGGYPHQVTLSSRQSVRELSWSPDGNTFAFTADQDGDEFTKVYL
                     VPSRGGQPVPVAVADQVQYSLLGWSPNGQLIAYAGNDREPANQDVIVWNVETGSEHRI
                     MATGGLLFPASWSPDSARMLVIDAHSNTNVDIHVVEVETGASRLLTPHEGEITYYPVG
                     WTADSSGFYFLSDEDREFTALGLFHLESGTREWIETPDWDIELAVTSQDGRRLVWAVN
                     EDGYSRLFTRDL"
     gene            complement(960..1763)
                     /locus_tag="VFQ54_05675"
     CDS             complement(960..1763)
                     /locus_tag="VFQ54_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0164516.1"
                     /translation="MSDLPGYMHTFGVYLRFQFRNEWRTTLFWTIGLSIYAGLIVSIY
                     PSVKGAVNLEAIPEGLRTAFNINDFTQLASFLSSQLLGVIMPLLLPFYGMIMLSNVVA
                     GAEERGRLDILLGNPIPRWQPIVGSFFVVAVYLLGIVIVLGGVIWSLARFLDLTLTIE
                     QAMRATFALWPTSLAFGALALFVSTRVRQRSLALGLPAAIVFLMYLFNVIGRLADSVR
                     WIRWTSAYNYYGTAITDGIWWPGMITLLAAAVILVVLAIVSFNRRDIYA"
BASE COUNT          368 a          516 c          608 g          320 t
ORIGIN      
        1 cgagatcccg ggtgaagagg cgggagtagc catcctcatt gaccgcccag accagacgac
       61 ggccatcctg ggatgtcacc gcgagctcga tgtcccagtc gggggtctcg atccattccc
      121 tggtcccgct ctcgaggtgg aagagaccga gcgcggtgaa ctcgcggtcc tcgtccgaga
      181 ggaaatagaa tccggaactg tccgccgtcc atccgaccgg gtaatacgtg atctctccct
      241 cgtgcggggt aaggagacga gacgctcccg tctcgacctc gaccacgtgg atgtcgacgt
      301 tcgtgttgga atgggcgtcg atcaccagca tccgagcgct gtctggcgac catgaggcag
      361 ggaacaacag gccgccggtc gccatgatcc ggtgctctga tcccgtctcg acgttccaga
      421 caatgacgtc ctgattggcc ggctcgcggt cgtttcccgc gtaggcaatg agttgtccgt
      481 tgggtgacca gccgaggagg gaatactgca cctggtcggc gacggcgacc ggtaccggct
      541 gaccgccgcg ggaaggtacg agatagacct tcgtgaattc atcgccgtcc tgatcggcgg
      601 tgaaggcgaa ggtgttcccg tcgggggacc aggagagttc acgcaccgat tgccggctgg
      661 aaagcgttac ctggtgcgga tagcctccgt cgctggattg gcgccaaagg ttgaactgtc
      721 cggatgtgtt gaccgagtaa gcgacctcgg acgcgtcggg ggagaacgca agtgtcccct
      781 ggaaacgccg gatggccgtg aattgctcga acgttggcaa gggcgcggat gcaggtgcag
      841 ccttcgagag gtgattggag ttcacggcct accccttctg tttgatttcg ccagactccc
      901 aggcgggagc atgattgccc tggattctac attcgtctgt cgtttcgtcg atcgtcggct
      961 caggcgtaga tatcgcgccg gttgaagctg acgattgcca ggacgaccag gatcaccgcc
     1021 gcggcgagca gggtgatcat cccgggccac cagatgccgt cggtgatcgc ggtgccgtag
     1081 tagttgtagg cagatgtcca gcggatccac cgcacgctgt cggcgagacg cccgatcaca
     1141 ttgaagaggt acatcaggaa gacgatcgcc gccggcaatc ccagcgcgag cgaacgctgt
     1201 cgtacgcggg tggagacgaa gagcgcaagc gcgccgaagg cgaggctggt cggccagagc
     1261 gcgaaggtcg cgcgcatcgc ctgctcgatc gtgagtgtga gatcgaggaa ccgcgcgagg
     1321 ctccagatca cgccgccgag gacgatcacg atcccgagca ggtagaccgc caccacgaag
     1381 aagctgccga cgatcggctg ccagcgggga atcgggttgc cgagcaggat gtcgagtcga
     1441 ccgcgttctt cggcaccggc gacgacgttc gagagcatga tcatcccata gaacgggagg
     1501 aggaggggca tgattacgcc caggagctgg ctggagagaa atgatgcgag ctgcgtgaag
     1561 tcgttgatgt tgaacgccgt ccgcagtccc tcgggaatcg cctccagatt gaccgcgccc
     1621 ttgaccgacg gatagatcga gacgatcagt ccggcataga tgctgaggcc gatcgtccag
     1681 aagagggtcg ttcgccattc gttccggaat tgaaagcgga ggtagacacc gaaggtgtgc
     1741 atgtatccgg gcaaatcaga caaccttacg cctcgtgtgc cagatcccgg tgacgaaccg
     1801 ccaggagaag ga
//
LOCUS       DASTMD010000390         1783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2179579, whole genome shotgun sequence.
ACCESSION   DASTMD010000390 DASTMD010000000
VERSION     DASTMD010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179579"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            764..1657
                     /gene="sdhC"
                     /locus_tag="VFQ54_05680"
     CDS             764..1657
                     /gene="sdhC"
                     /locus_tag="VFQ54_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEU0164517.1"
                     /translation="MAYVSTEQGRAGLGPTASPGAERLMWYFLRVSGLALVFLAGGHI
                     FITHYLNVPSDTTFDFVSKRWSNPLWRSFDTLLLLAALWHGLIGLRFSIKDFLRSPGA
                     RQIAMSLLWVFGAVFTVVGLITIVTFDEQMSRDNTGPLSGQMWIANTLGAFLVILAIC
                     TYVGVIALIVWVVNSLRSGIVPIYNGDVGQYAWVLHRATGIGILGFLLVHVIDIMLIG
                     LGRDVYDHSVHFYGNWFIIPMEILLVGAVIYHTLNGLRIIAINFSSRGPSMEKKYFWW
                     VLIGSVVLTLPSAIIILINEL"
BASE COUNT          309 a          576 c          551 g          347 t
ORIGIN      
        1 cgttggagcg attccctcga ctgcgctccg gtgcagctac tcgatgcggc tgccgatccg
       61 ctcccgggga gcgccatccg tgcccgattg gcgttggtgt ccgctgcggc ggaccggctt
      121 cgccggggat ttctccattc cgctccgctt cagtcgaaat gacaaacctt cgtgcgacag
      181 cgctggagat agactcacgc ccgacaaacc ccggtcgtca tgaacagcgt aaacggcgcc
      241 gaattcgtgt catcaaggcc gtttgcgcag cagtcgtgag tacgggggac ttctatgtcc
      301 ccctcaagac acgccccccg caacgcgctt cgcagatctg ccgcgcttcg ccctggaata
      361 tcgtcgccga cgttgccgtt ggagtccgcc tacggcggat cggcgtcgcc gggggggtcc
      421 ttcggcatgc tcaggatgat gggcggagag gtatcgcggt cgaaacacgc ggcgactggc
      481 cggtccctcg acaagttcgg gaggacggtt ggaggggcgg gatgtcaaac cgcaggggat
      541 ccggtcgtgc tggcagaccg agcggaccga tgagaatcgc tcgcgcctcg atacccacgt
      601 tcgggagcac catcgtacaa tacgcgccag tgaacgatcg gacgacagtc ccgaatcccg
      661 gtgcgacgga gtaccaggga ggtcggatac gggcgccaac gagaatgacc tcgcttgatc
      721 gggcccggtc ggacgaacac catgtgggga ggatgcggca gacatggcgt acgtatcgac
      781 ggaacaaggc cgcgccggcc tgggtcccac ggcgagcccg ggcgcagaac ggttgatgtg
      841 gtatttcctc cgcgtctccg gtctggcact ggtctttctg gctggtggtc acatcttcat
      901 cacccactac ctgaacgtcc cgtccgatac gaccttcgat ttcgtctcca agcgctggtc
      961 gaatcccctc tggcgatcct tcgacacgct ccttctcctc gccgcgctct ggcacggact
     1021 gatcgggctc cgcttctcga tcaaggactt cctgcgaagc cccggtgccc gccagatcgc
     1081 gatgagcctg ctctgggtct tcggcgcggt cttcacggtc gtcggcctga tcacgatcgt
     1141 gaccttcgac gagcagatgt cgcgcgacaa caccggcccg ctctccgggc agatgtggat
     1201 cgcgaatacg ctcggcgcct tcctcgtgat cctcgcgatc tgcacgtatg tcggcgtgat
     1261 cgcgctcatc gtctgggtcg tgaacagcct ccgctccggt atcgtgccga tctacaacgg
     1321 tgacgtcgga cagtacgcgt gggtcctgca ccgggcaacc ggcatcggca tccttggctt
     1381 tctgctcgtg catgtcatcg acatcatgct gatcggcctc ggtcgcgatg tctacgatca
     1441 ctcggtgcac ttctacggca actggttcat catcccgatg gagatcctgc tcgtcggcgc
     1501 ggtgatctat cacacgctga acggcctccg gatcatcgcg atcaacttct cctctcgtgg
     1561 accgagcatg gagaagaagt acttctggtg ggtgctcatc ggctccgtcg tgctgacgct
     1621 cccgagcgcg atcatcatcc tgatcaacga gctgtagaac ccgagcgggc ggacgaagcc
     1681 ggcggaaggc aggacatcgt tgaagccaac gagtaactcc atccgaagac cgggcctcca
     1741 atggcgggac agagacgtcc cgcccggttc acgaccgctg ggc
//
LOCUS       DASTMD010000391         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1376418, whole genome shotgun sequence.
ACCESSION   DASTMD010000391 DASTMD010000000
VERSION     DASTMD010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376418"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFQ54_05685"
     CDS             complement(<1..202)
                     /locus_tag="VFQ54_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU0164518.1"
                     /translation="MATYQVSLIPGDGIGPEVTEAARRVLEATGIGFDWDYRDAGLEA
                     LEKHGDVLPEATLDSVRKNGLGL"
     gene            398..1969
                     /gene="gpmI"
                     /locus_tag="VFQ54_05690"
     CDS             398..1969
                     /gene="gpmI"
                     /locus_tag="VFQ54_05690"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEU0164519.1"
                     /translation="MTDHKKPVLLAILDGWGIGRGEPGNAVLAADTPNLDRLLEKYPH
                     ASLVTSGEAVGLPEGQMGNSEVGHMNLGAGFVVYQWITRIDKAIADGELATNETLQKA
                     LAHAREGGGTVHFLGLVGHGGVHSHTRHLLALVDAAVAAGNEKILVHAFTDGRDTSPT
                     SGDGFVAEVEEKLKAIGKGRIASVSGRYFAMDRDHRWDRTQKAFDAIVHANGPTADDA
                     RAAIREAHDAGTTDEFIVPTVIRDKDGSTHHVDADDSVIFFNFRSDRGRQLTEAMSVA
                     DFDGFDRGDFKVDLDVTTLTTYEESLPVHVIFPPHDVKEPLAKVISDAGMTQFHTAET
                     EKYPHVTFFLNGGREEPFPGEERHMVQSPKVATYDLQPEMSAAGVKDGVLEAIRSGTY
                     DFIIVNFANDDMVGHTGSIPAVIEAVETVDTCIGEVVDALLAAGGSGIITADHGNAEE
                     MIDRKTGGPMTSHTTNPVPVILVAPDDSPRRHATLRTDGVLSAVTTTILDLLDLEPPS
                     DMTQPGLIVGEERSA"
     gene            1966..2556
                     /locus_tag="VFQ54_05695"
     CDS             1966..2556
                     /locus_tag="VFQ54_05695"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU0164520.1"
                     /translation="MIQGKRVSFRAFSRDDIPTLVRWQNDGEVMQYWGERQPIVAPSA
                     FDAEFDQGGRFARFDERGELCLCDENGRPIGRFGYKGAAFRDRHVEIFLYIGERDAQG
                     KGYGTEAIVLMLNWLFIHRGMHRVWLTVQAENARAIHTYEKVGFIREGIFREHYFRDG
                     AFRDEIIFGMLAREFTARYRPEETGLVVSGEIPAIS"
     gene            2584..>2901
                     /locus_tag="VFQ54_05700"
     CDS             2584..>2901
                     /locus_tag="VFQ54_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU0164521.1"
                     /translation="MKLYSWNVNGIRAAIKSGSLQEMIATHAPDVLCLQEVKAGPHQV
                     TLDLPDYSAYWNPADKAGYSGTAIFSRVEALTIQPGLPADLVEQFGLADDPFGNPNAE
                     GRVI"
BASE COUNT          561 a          937 c          881 g          522 t
ORIGIN      
        1 tcaggcccag accattcttg cgcaccgaat cgagcgtcgc ctccgggagg acatcgccgt
       61 gcttctcaag cgcctccaac ccggcatcgc ggtagtccca gtcgaacccg atcccggtcg
      121 cctcgagcac gcggcgcgcg gcttccgtga cctctggtcc gataccatcc ccgggaatca
      181 gcgatacctg atacgttgcc atgtcatctc cttgtttcaa tcctcaccgg gtccgaagac
      241 cgagtgccac ggagtcttgc gaatgaccgc cctgttagcc cgagcattgt cgcatgccgc
      301 ttccgggcac gtacattcga ccgatcgacc acaatagcga gagcactcga tagcattcct
      361 caacgacatc aagttcgaac cacaggagtc tcacggaatg acggaccaca agaagccagt
      421 tctcctcgcg atcctcgacg gttgggggat cggtcgcggc gagcccggca acgccgtcct
      481 tgcggcggat acaccgaatc tcgaccgcct tcttgagaag tatccccatg cctcactcgt
      541 tacctccggt gaagccgtcg gcctgcccga gggccagatg gggaattccg aggtggggca
      601 catgaacctg ggcgcgggtt tcgttgtcta ccagtggatc acgcggatcg acaaggcgat
      661 cgccgatggc gagctcgcca ccaacgaaac cctccagaag gcgctcgctc acgcccgcga
      721 gggcggagga accgtccatt ttctcggcct ggtcggccac ggcggagttc acagtcacac
      781 ccgtcacctg cttgccctgg tcgacgccgc cgtcgcggcc ggcaacgaga agatcctcgt
      841 tcacgccttc accgatgggc gcgacacttc gccgacgagc ggtgacggat tcgtcgcgga
      901 agtcgaggag aagctgaagg cgatcggcaa gggccggatc gcctcggtgt cgggtcgcta
      961 cttcgcaatg gatcgcgacc accgctggga tcgcacgcag aaggcgttcg acgcgatcgt
     1021 ccatgccaac ggtccgaccg cggacgacgc acgagccgcg atccgcgagg cacatgacgc
     1081 tggtacgacc gacgagttca tcgtcccgac ggtgatccgg gacaaggacg gttcgacgca
     1141 ccatgtcgac gccgacgaca gcgtgatctt cttcaacttc cgttcggacc gcggtcgcca
     1201 gctgaccgag gcgatgtctg tcgccgactt cgatggcttc gaccgcggcg acttcaaggt
     1261 cgacctcgat gtcacgaccc tcacgacgta tgaggagtcg ctccccgtcc atgtgatctt
     1321 cccaccgcac gatgtgaagg agccgctggc gaaggtgatc agcgacgccg ggatgacgca
     1381 gttccacacc gccgagaccg agaagtaccc ccacgtcaca ttctttctga acggcggccg
     1441 tgaggagcca ttccccggcg aggaacggca catggtgcag tcaccaaagg tcgccaccta
     1501 cgatctccaa ccggagatga gcgcggccgg ggtgaaggac ggtgtgctgg aagcgatccg
     1561 ctccggcaca tacgacttca tcatcgtgaa cttcgccaac gacgacatgg tcggtcacac
     1621 gggatcgatc ccggcggtga tcgaagcggt cgagaccgtt gacacctgca tcggcgaggt
     1681 cgtcgacgcc ctgctggcgg ccggtggaag cggcatcatc accgcggatc acggcaacgc
     1741 agaggagatg atcgatcgga agacgggtgg cccgatgact tcgcatacga ccaaccctgt
     1801 ccccgtcatc ctcgtcgccc ccgacgacag tccgcgccgc cacgcgacgc tccgcaccga
     1861 tggcgtcctc agcgcggtca ccacgaccat tctcgacctg ctcgacctcg agccgccatc
     1921 cgacatgact caaccgggcc tgatcgttgg cgaggagagg tccgcgtgat ccagggcaag
     1981 cgcgtctcct ttcgcgcctt ctcccgcgac gacatcccga cccttgttcg atggcagaat
     2041 gacggcgagg tcatgcagta ctggggcgag cgccaaccga tcgtcgcacc gtctgcattt
     2101 gacgccgagt tcgaccaggg aggacgtttc gctcgtttcg acgagcgcgg cgaactctgc
     2161 ctttgcgatg agaacggtcg tccgatcggt cgcttcggat acaagggcgc ggcgttccgg
     2221 gatcgacacg tcgagatctt cctgtacatc ggcgagcgtg atgcccaggg aaagggctac
     2281 ggcactgagg caattgtcct gatgctgaac tggctcttca tccatcgtgg aatgcatcgc
     2341 gtctggttga ccgtccaggc cgagaacgcg cgtgcgattc acacctatga gaaggtcggg
     2401 ttcatccgtg aagggatctt ccgcgaacat tatttccggg acggcgcctt cagggacgag
     2461 atcatcttcg gcatgcttgc ccgggagttc accgcccggt atcgtccgga ggagacgggc
     2521 ttggtcgtct ctggtgaaat ccccgcgatc tcgtgaatcg tctttgatgt gaagggaaca
     2581 gcgttgaagc tctattcgtg gaatgtcaac ggtattcgcg cggcgatcaa gagtggaagc
     2641 ctccaggaaa tgatcgccac gcacgcaccg gacgtgcttt gcctgcagga ggtcaaggcc
     2701 ggacctcacc aggtgacgct tgatctgccg gactattctg catattggaa tccggccgac
     2761 aaagccggat acagcggcac cgcgatcttc tccagggtcg aggcgctcac gatccaaccc
     2821 gggcttccgg cggatctggt cgagcagttc ggcctcgcgg acgacccgtt cggcaatccc
     2881 aatgccgagg ggcgagtgat c
//
LOCUS       DASTMD010000392         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2715198, whole genome shotgun sequence.
ACCESSION   DASTMD010000392 DASTMD010000000
VERSION     DASTMD010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2715198"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="VFQ54_05705"
     CDS             <1..1415
                     /locus_tag="VFQ54_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0164522.1"
                     /translation="ARETEPALIGHDQILWMQRLDEDIANLRLALQIALDHPKEGGVN
                     GLQIASGLWRYWLVRGQLTEGCMWLERMTQLPAEFPPVLHAGALNNLGNLYLELGQLE
                     SARDHYIRSRDLYANVGDLYGVGDELNNLGLVELISGHFGESRRILEDALEMRRKYAD
                     RLALPTTLCNLGDIAMFEGDFDTAVRYHAEAYDIRIEHGNKRGLALSCNALGLIAYYR
                     GDLPTADRWFAEGMGYAMELGDAYSKSLLQVDLGLVATARQNLIPALELTTTALRTLH
                     QMGSRRMMAEALDGLAEAAILARRYELAARLLGGAKLLRDEHAIAIATPNRAGFPTIE
                     ARLSRWLGDDEFAAQFASGRRAPQEAVFAEALALLEDVRTNGVPASADDADPGAVAID
                     EARLGEIGLTAREREILSLLVHGFTDRQIADQLYISPRTAMTHVGKVLNKLGVNKRAL
                     AASVALRDHLVDPSAPLPHH"
     gene            1598..>2086
                     /locus_tag="VFQ54_05710"
     CDS             1598..>2086
                     /locus_tag="VFQ54_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0164523.1"
                     /translation="MFETSAGPTTWTLTSLPFPLTSLVGRQRELDAAIGLLQRPDLRL
                     LTLTGPGGVGKTRLSLAIGTAIEEMGYPVAFVNLAPIADPTLVLPEIASAIGVSAGDK
                     VTLMSRIVQTIGYQRLLFVIDNFEQVVAASVHLSTLLSLCPGTKALVTSRMPLHIQGE
                     QEL"
BASE COUNT          370 a          672 c          671 g          373 t
ORIGIN      
        1 tcgcgcgcga aacggaaccg gcgctcatcg gtcacgacca gatcctgtgg atgcagcggc
       61 tcgacgagga tatcgccaac ctccggctcg cgctccagat cgcgctcgat cacccgaagg
      121 agggaggcgt caacggcctc cagatcgcct cgggtctgtg gcgctactgg ctggttcgcg
      181 gtcagttgac ggagggatgc atgtggctgg agcggatgac gcagctcccg gccgaattcc
      241 ccccggtgct tcacgccggt gccctgaaca atctcggcaa tctctacctc gaactcgggc
      301 aactcgaatc cgcgcgcgat cactacattc gcagccggga cctctacgca aacgtcggcg
      361 atctctacgg cgtcggggac gaactgaaca atctcggcct cgtcgagttg atcagcggcc
      421 atttcggcga atcccggcgg attctcgagg acgccctgga gatgcgtcgg aagtacgccg
      481 accgtctcgc cttgccgacc acgctctgca acctcggcga tatcgcgatg ttcgagggtg
      541 atttcgatac cgccgtccgc tatcacgccg aggcgtacga tatccggatc gagcacggga
      601 acaagcgcgg tctcgcgctt tcctgcaacg cgctcggtct gatcgcgtac taccgtggcg
      661 atctcccgac ggccgaccgg tggttcgcgg aagggatggg ctatgcgatg gaactcggcg
      721 atgcctacag caaatcactg ctccaggtcg atctcggtct cgtcgcgacc gcgcgccaga
      781 acctgattcc ggcgctggaa ctgacgacga cggccctgcg cacgttgcat cagatgggat
      841 cgcgccggat gatggcggaa gcgctggacg gtctcgccga ggcggcgatc ctggcgcgac
      901 gctacgagct tgccgcgcgc ctcctcggcg gcgcgaagct cctgcgggac gaacacgcga
      961 tcgcgatcgc cacgccgaac cgcgcgggct tcccaacgat cgaggcgcga ctgagtcgct
     1021 ggttgggtga cgacgagttc gccgcacagt tcgcctcggg acgccgggca ccacaggaag
     1081 cggtctttgc cgaggcgctc gcactgctcg aggatgtccg cacgaacggt gttccggcgt
     1141 cagctgatga cgccgacccg ggagccgtcg cgatcgacga ggcgcgactc ggtgagatcg
     1201 gccttaccgc acgcgagcgg gaaattctca gcttgcttgt ccatggcttt accgatcgcc
     1261 agatcgcgga tcagctctac atcagcccgc gcaccgcaat gacacatgtt ggcaaggtat
     1321 tgaataaact cggagtgaac aaacgcgcct tggccgcgag tgtggcgttg cgcgatcatc
     1381 tggtcgatcc gtcggcgccg ctaccgcacc actagcgacg tgttcgtcgg atcgcgggac
     1441 tttggcggcc aagacaccat cggcgtgtct gatgcgtccg ggttgagaac gggacgcaca
     1501 ttttccgggc aacattgtcg cgtaggatgg gagagcgcgg tgggcgcatt ccatcatcgg
     1561 cggtgaactg ttcatggcag ccaacgagga gaatgacgtg ttcgagacgt cggccgggcc
     1621 gacgacgtgg acactcacga gcctgccgtt cccgctgaca tcgctcgtcg ggcgtcaacg
     1681 agaactggac gccgccatcg gcctgcttca gcgacccgat ctgcgtctcc tgacgttgac
     1741 cggtcccggc ggcgtgggca agacgcggct ctcactggcg atcggcaccg cgatcgagga
     1801 gatggggtat ccggttgcct tcgtgaacct ggcgccgatc gccgatccaa cgctcgtcct
     1861 cccggaaatc gcctccgcca tcggggtcag cgccggtgac aaggttacgc tgatgagcag
     1921 gatcgtccag acgatcggct atcagcggct cctcttcgtg atcgacaatt tcgagcaggt
     1981 cgtcgcggca agcgtgcatc tctccacgtt gctttccctc tgcccgggga cgaaggcgct
     2041 cgtcacgagc cggatgccgc tccatatcca gggcgagcag gaactc
//
LOCUS       DASTMD010000393         1764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3071980, whole genome shotgun sequence.
ACCESSION   DASTMD010000393 DASTMD010000000
VERSION     DASTMD010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3071980"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /gene="bamA"
                     /locus_tag="VFQ54_05715"
     CDS             complement(<1..1619)
                     /gene="bamA"
                     /locus_tag="VFQ54_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEU0164524.1"
                     /translation="MIGCLSPAFAESITVEGNHRVDAETIRSYFTGTDSAHVAEGVKG
                     LKATGLFTSVRAQQGRGGLVVSVTENGIVNRVAFEGNSKVKSEVLQAEVLSKSRSAYD
                     PAVVQADLDRIREVYRRAGRADATVTSRTVDLPNGKFDVVFTVDEGSKTGVKEITFAG
                     NQAFSERKLRGLMQTTEMNLLSFFKTSDVYDPDKIATDEELIRRFDLRNGYADFRIVN
                     TDVKFDAEQKGYLVKITVDEGQPYKVAAVSVDSHLRTVSSDELSPDLRMEVGDVYDGD
                     QVEKTVDAITRDTGRRGLPFSQVRPRGDRNPANHTVSVQFVVDDGPRVYIERINIHGN
                     TRTRDYVIRREFDVGEGDPYNKVLIDRAERRLNALGFFKKIRITNAPGSSPDRVIVDV
                     DVEDQPTGSFSISGGYSTSDGFIAEVAVTETNFLGRGDYVRVAAQEGQYARGVDLSFT
                     EPYLFDTRIAGGVDLFAKQNDNTQYTEYTTFVTGGTLRFGVPVTDEFSVSPHYSIYNT
                     KITIPNKAGSAYDDCTFPLSGITPGYGGSSGA"
BASE COUNT          337 a          530 c          539 g          358 t
ORIGIN      
        1 gcgccggacg atccaccgta accgggtgtg atgccggaga gcgggaaggt acagtcgtca
       61 taggccgagc ccgccttgtt cgggatggtg atcttggtgt tgtagatcga atagtgcgga
      121 gagacggaga attcatccgt aaccggcacc ccgaagcgca aggtcccacc cgtcacgaag
      181 gtcgtgtact cggtatattg cgtattgtcg ttctgcttcg cgaacagatc gacgccgccc
      241 gcgatgcgcg tgtcgaacag atagggttcc gtgaacgaca gatcgacacc gcgcgcatat
      301 tgaccttctt gggcggcgac ccggacatag tcgccgcgac ccaggaagtt cgtctccgtc
      361 acggccactt ccgcgatgaa gccgtcgctc gtcgaatagc cgccagagat cgagaacgat
      421 cccgtgggct gatcctcgac atcgacatcg acgatgacgc ggtcgggtga ggagccgggc
      481 gcattggtga tgcggatctt cttgaagaac ccgagcgcat tcagtcggcg ctcggcccga
      541 tcgatcagca ccttgttgta ggggtcgcct tcgccaacat cgaactcgcg gcggatcaca
      601 taatcgcggg tgcgagtgtt gccgtggata ttgattcgct cgatgtagac gcgcggacca
      661 tcgtcaacca cgaactgaac cgagaccgtg tgattggcgg ggttgcggtc gccgcgcggc
      721 cgaacttgcg agaagggcag gccgcggcgt cccgtgtcgc gcgtgatcgc atcgacggtc
      781 ttctccacct ggtcgccgtc gtagacatcg ccgacttcca tacggaggtc cgggctcaat
      841 tcgtcgctcg agaccgtacg cagatgcgag tcgaccgaca ccgccgccac tttgtagggc
      901 tggccttcat cgacagtgat cttgacgagg taacccttct gctcggcgtc gaacttcacg
      961 tcggtgttga cgatccggaa atcggcatag ccgttgcgca gatcgaagcg gcggatcagt
     1021 tcctcgtcgg tcgcgatctt gtccggatca tagacgtctg acgtcttgaa gaacgacagg
     1081 aggttcatct ccgtggtctg catcagacca cgcagcttgc gctccgaaaa cgcctggttg
     1141 ccggcgaagg tgatttcctt gacgccggtc ttgctgcctt cgtcgaccgt gaagacgacg
     1201 tcgaacttcc cgttcggcag gtcgacggtc cgtgacgtca ccgtcgcatc ggcgcggccg
     1261 gcgcggcgat acacttccct gatccgatcg agatccgcct ggacgacggc agggtcgtag
     1321 gcgcttctcg atttcgaaag cacctcggcc tgcaggactt cggacttgac tttgctgttg
     1381 ccctcgaagg caacgcgatt cacgatcccg ttctcggtga ccgagacaac aagaccgccg
     1441 cggccctgct gggcgcgaac ggaggtgaac aacccggttg ccttgaggcc cttgacgcct
     1501 tctgcgacat gggccgagtc cgttccggtg aagtacgagc ggatcgtctc cgcgtcgacc
     1561 cgatgattgc cttcgacggt gatcgattca gcgaacgcgg gcgacaggca cccaatcaaa
     1621 gccgaaacgc acagtacgaa tatagcaact cgatgcaaaa cgccgcgatg aagttgcatg
     1681 tatgaccggt cctgtccttc ttgaagcggc ttgttccgct cctctcccgg gccccttccc
     1741 gaaattctga atgaatttcg tagg
//
LOCUS       DASTMD010000394         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2180039, whole genome shotgun sequence.
ACCESSION   DASTMD010000394 DASTMD010000000
VERSION     DASTMD010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180039"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFQ54_05720"
     CDS             <1..219
                     /locus_tag="VFQ54_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164525.1"
                     /translation="SLRHYVFADIGTGIARTDEILGLLVGRAAIPWIVIEQDTCEDDS
                     TITAARNLQAVHRMIDMKLARESDAKPG"
     gene            221..1099
                     /gene="iolB"
                     /locus_tag="VFQ54_05725"
     CDS             221..1099
                     /gene="iolB"
                     /locus_tag="VFQ54_05725"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEU0164526.1"
                     /translation="MILHSDTTAATALKIEITPESAGWDYTGLVVVRLLSGEIWTDAF
                     PDDEICIVTLWGSAIVESGGASWEVSREGGLFAAKPTALYLPVGSEITMTAGSDCEIA
                     ITSSRARVAYPARLITPGEIDVEIRGAGNAARQINHIIKPDFPAHRLLVVEVFTPSGN
                     WSSYPPHKHDISRMPRESKLEEIYYYRIDQPDGFGLQRLYAADDSFDYAWVIQDGDVM
                     LVPEGYHAFVVAHGYTGYYLNILAGDEPVRTMQPADDPRYGWVRGTWSDDLSAGITGW
                     RDIDERVNHGAGSRPE"
     gene            1130..>1816
                     /locus_tag="VFQ54_05730"
     CDS             1130..>1816
                     /locus_tag="VFQ54_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0164527.1"
                     /translation="MTTIRLTVAQALVTYLGAQYTERDGHRQKLFEGVFGIFGHGNVT
                     GIGQALEELRDVIPYYRAQNEQAMVHTAVGFAKMKNRLQTFACTSSVGPGATNMITGA
                     ALATVNRIPVLLLPGDTFGSRRPHPVLQQLEYPLGQDVSVNDAFRPVSRYWDRILRPE
                     QLLSSLPEAIRVLTDPDETGAVTLALPEDVQTEAYDFPAEFFAERTWRVRRAVPDQVD
                     LAEAVEWINAA"
BASE COUNT          353 a          600 c          543 g          320 t
ORIGIN      
        1 tccctccggc attacgtctt tgccgacatc ggcaccggca tcgccagaac ggacgagatt
       61 ctgggactac tggttggtcg ggctgcgatc ccctggatcg tgatcgaaca ggatacctgc
      121 gaggatgact cgacgattac tgcggcgcgg aatctgcagg ccgttcatcg catgatcgat
      181 atgaagcttg cgagggaatc agatgccaag ccgggatgaa atgattctgc attcggacac
      241 taccgccgcg accgcgctga agatcgagat cacacccgaa agcgccggtt gggattacac
      301 gggactcgtc gtcgtccggc ttctctcggg agagatctgg accgacgcct ttcccgacga
      361 cgagatctgc atcgtcaccc tctggggaag cgcaatcgtc gaatcaggcg gagcgtcctg
      421 ggaggtttcg cgtgaaggcg gactcttcgc cgccaaaccg accgcgctct atctcccggt
      481 gggaagcgag atcacgatga ccgccggctc cgactgcgag atcgcgatca cctcctcccg
      541 cgcgcgcgtc gcctaccctg cccggctcat cacgccaggc gagatcgatg tcgagattcg
      601 gggcgccggc aacgccgccc gccagatcaa tcacatcatc aagcccgact tcccggcgca
      661 caggctgctt gtcgtcgagg tcttcacgcc cagtggcaac tggtccagct atcccccgca
      721 caagcacgac atcagccgga tgccgcgcga atcgaagctc gaggagatct actactaccg
      781 gatcgaccag cccgatggat tcggactcca acgcctctat gccgcggacg actcgttcga
      841 ctacgcgtgg gtgatccagg atggcgatgt catgctcgtt cccgagggat atcacgcctt
      901 cgtcgtcgcc catggctaca ctggctatta cctgaacatc ctcgccggtg acgaacccgt
      961 gcgaaccatg caacctgccg acgacccccg ttacggctgg gttcgtggca catggagcga
     1021 tgacctgtcc gccggtatca cgggctggcg ggacatcgat gagcgcgtca accacggagc
     1081 ggggtccaga cccgaatagt gcagatagcg agaagacaga ggatcaacga tgaccacgat
     1141 cagactgact gtcgcccagg cgctcgtgac ctaccttggc gctcagtaca cggaacgcga
     1201 tggacaccgg cagaagctct tcgagggcgt ctttggcatc ttcggccacg gcaatgtcac
     1261 cgggatcggt caggcgctcg aggaacttcg ggatgtcatc ccctactacc gcgcccagaa
     1321 cgaacaggcg atggttcaca cggctgtcgg cttcgcgaag atgaagaacc ggctccagac
     1381 cttcgcctgt acctcgtccg tcggcccagg ggcgacgaac atgatcaccg gcgccgccct
     1441 cgcgacggtg aaccggattc cggttctgct gcttccagga gacaccttcg gcagccgccg
     1501 tccgcacccg gtcctccagc aactcgagta tccactcggc caggacgtct cggtcaacga
     1561 cgcgtttcgc ccggtctcgc gctactggga ccgcatcctc cgcccggagc agctcctgtc
     1621 gtcgttgccg gaagcaatca gggtattgac cgatccggac gagacgggcg ccgtgaccct
     1681 tgcgctgccg gaagacgtgc agacggaggc atatgacttc cccgccgagt tcttcgccga
     1741 gcgcacatgg cgggtccgcc gcgcggtacc ggatcaggtc gatctcgccg aggccgtcga
     1801 atggatcaac gccgcg
//
LOCUS       DASTMD010000395         4799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1645365, whole genome shotgun sequence.
ACCESSION   DASTMD010000395 DASTMD010000000
VERSION     DASTMD010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645365"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..2270
                     /locus_tag="VFQ54_05735"
     CDS             237..2270
                     /locus_tag="VFQ54_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0164528.1"
                     /translation="MSDELEDQETTPARNVTRRRVLQASAGASLLAGAVALGTRGSGA
                     LAQDATPAASPAATPAATGAAALPLVPPEVTTYANDWPVAQGDLASTRVAVGGTIDST
                     NVAQLGVAWEIPLEASSGFGAITSNPVVQGDVVYIIDNNATVQAVKKETGEVIWKNEY
                     KSNTFGPNGVAIGYGVLALVVGDAAEVVAVKPEDGSELWRTTLTNHNALGITMAPAIS
                     QGWVIVSTEPGGNSKGTYEGGANGVVYALDVTNGEIIWSWDTIQDDLWGNFRVNSGGG
                     LWYPPAIDDKGVLYMGIGNPAPWPCNAQYPNASSRPGENDYANNLVALDPTAGQVLWN
                     INIKPRDLYDHDNQQSPVLGTVSIGGVDAEVVFTSGKHGYVAAAHRQSGQEYWRVPVG
                     KHKNDGLLVLPKEYIEIYPGTLGGVESPMAFAEGILYVAALNYPASMNESGFDFLNSK
                     PYSAATSNLLALDGATGRAIWDIELPYGIAGPGPVITKDLLFVGSLDGVVRAFTTKDG
                     KQVWTSQTSAGLNSAFAVAGDMLFVPAGSVIAPSADTPSPAPGYHAAVIAYKIGATGK
                     PTLAPAAAATPEGLLVPAEPTNGEITVTAVDLAFQPKNFSIDANTDVKITLDNKGVLA
                     HDFVIDDPKVDSGMVNGGEQTTFTVNLPAGDYKFYCSVEGHAAAGMVGVLTAK"
     gene            complement(2376..3287)
                     /locus_tag="VFQ54_05740"
     CDS             complement(2376..3287)
                     /locus_tag="VFQ54_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEU0164529.1"
                     /translation="MSVSDDEVVIAFIGGGPRVTGLIERITANAQLVAPRTSVVVLMY
                     DTSPAGVEHPMRDDWSLIATLGRRAEEITMFSDASCLLQGPVVTGPSLAEWIGSVRSG
                     TIRFDPPDFPDQTEMRSSGSHRLPMHRIVDWYLRWFFGEIRRGAPENVRINEIRARVN
                     RIYPHGDRNVLDVVYESGEHHAQPVDLVVLGLDEGSVDLRRTHGERKNGDTAGRSTAG
                     EDRARPLDAVVQQRLASPAIAVRRSSTILDLARIPGSEPFARPGTNARFFRVNDRVAR
                     WLLQTSAGRVSANGAGSLRTVAGRRGA"
     gene            complement(3414..4799)
                     /locus_tag="VFQ54_05745"
                     /pseudo
     CDS             complement(3414..4799)
                     /locus_tag="VFQ54_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
BASE COUNT          862 a         1576 c         1512 g          849 t
ORIGIN      
        1 caggttccgg atgcggatga tgtgtcatga ccgatcccat ggttgtacag gcgtctcgaa
       61 tcgttgggat catggtactt cgtcacatca acgtcgccgt catgttgaca agctgcaagc
      121 aaatccgtat gttacaagct atcaagcgga aaatatattc cgtttctatg aagccacgcg
      181 gcttcacggg agcttcgggc agccggcccg gcacaacctc gacggggaga gaacgcatgt
      241 cggacgaatt ggaagaccag gaaacaacgc cagcgcggaa tgtcacacgt cgtcgcgtcc
      301 tccaggccag cgccggcgcc agcctgctcg ccggcgcggt ggcgctcggc acacgtggtt
      361 cgggcgccct cgcgcaggac gccacaccgg cggcctcgcc cgccgcgaca ccagccgcca
      421 ccggagcagc ggccctgccg ctggttccgc ctgaagtgac cacctacgcc aacgactggc
      481 cggtggcgca aggcgacctc gcgtcgaccc gcgtcgccgt gggcggcacc atcgactcca
      541 ccaatgtcgc gcagcttggc gtggcatggg agattccgct cgaggcgtct tccggcttcg
      601 gcgcgatcac ctcgaacccc gtggttcagg gcgatgtcgt ctacatcatc gacaacaacg
      661 cgacggtgca ggcggtgaag aaggagaccg gcgaggtcat ctggaagaac gagtacaaga
      721 gcaacacgtt cggaccgaac ggtgtcgcga tcgggtatgg cgtcctcgca ctcgtggttg
      781 gtgatgccgc cgaggtcgtc gcggtgaagc ctgaggatgg cagcgaactc tggcgcacca
      841 cgctcacgaa tcacaacgcc ctcggcatca cgatggcgcc ggcgatctcc cagggatggg
      901 tgatcgtcag cacggaaccg ggcggcaact ccaagggaac gtatgaaggc ggcgccaacg
      961 gtgtcgtcta cgcgctcgat gtcacgaatg gcgagatcat ctggtcgtgg gacacgatcc
     1021 aggacgatct gtggggcaac ttccgggtca actccggcgg tggcctctgg tatccgcccg
     1081 ccatcgatga caagggcgtg ctctacatgg ggatcggcaa ccccgcgccg tggccatgca
     1141 acgcccagta tccgaatgcc tccagccgcc cgggcgagaa cgactacgcg aacaacctcg
     1201 tcgcgctcga cccgactgcc ggccaggtgc tctggaacat caacatcaag ccacgcgatc
     1261 tctacgacca cgacaaccag cagtcgccgg tactcggcac ggtcagcatt ggcggtgtcg
     1321 atgccgaggt cgtcttcacc tcgggcaagc acggctacgt cgccgccgct catcgccaga
     1381 gcggtcagga atactggcgc gttccggtgg gcaagcacaa gaacgacggt ctgctggtgc
     1441 tgccgaagga gtacatcgag atctacccgg gaacgctcgg tggtgtggaa tcgccgatgg
     1501 ccttcgcgga gggaatcctc tacgtcgccg cgctgaacta ccccgccagc atgaacgaat
     1561 cgggcttcga cttcctcaac agcaagccat actccgcggc aacgtcgaac ctgctcgcgc
     1621 tcgatggcgc caccgggcgc gccatctggg atatcgagct tccctatggc atcgccgggc
     1681 cgggcccggt gatcaccaag gacctcctct tcgtcggctc gctcgatggg gtcgtccgcg
     1741 ccttcacgac gaaggacggc aagcaggtct ggacctcgca gacgagcgct ggcctcaact
     1801 cggccttcgc ggtggcgggc gacatgctct tcgtccccgc cggcagcgtg atcgccccct
     1861 cggccgatac cccgagcccc gctcccggtt accacgccgc ggtgatcgcc tacaagatcg
     1921 gcgcgaccgg taagccgacc ctggcgccgg cagccgccgc gaccccggag ggtctcctgg
     1981 ttccggccga gccgaccaac ggcgagatca cggtgaccgc ggtcgacctc gcgttccagc
     2041 cgaagaactt ctcgatcgac gcgaacacgg acgtgaagat cacgctggac aacaagggcg
     2101 tcctcgccca cgacttcgtg atcgacgatc cgaaggtcga ttccggcatg gtcaatggcg
     2161 gtgagcagac caccttcacg gtcaacctcc cggccggcga ctacaagttc tactgctcgg
     2221 tcgaaggcca tgccgcggca ggcatggtcg gtgtcctgac cgcgaagtag caaccatcgc
     2281 ttcggcgatc cgagcatcag cacacgcgat cccgggagga atcccgggat cgcgttctgc
     2341 cagaaccgcc ccagggcagt ttccaccctc gcaactcatg ccccgcgccg accggcgacg
     2401 gtcctcagcg aacccgctcc gttcgccgaa acgcgcccgg cagatgtctg cagcagccag
     2461 cgcgcgaccc gatcgttgac gcggaagaac cgcgcattcg tcccggggcg tgcgaacggt
     2521 tcggaaccgg ggatgcgggc gagatcgagg atcgtgctgc tccggcgcac ggcgatcgca
     2581 ggcgacgcga gccgctgctg gacgaccgcg tcgagcgggc gggcacgatc ttcccccgcg
     2641 gtcgatctcc cggcggtgtc cccatttttt cgctcgccat gcgttcgccg gagatcgacc
     2701 gacccctcgt ccaatccgag caccacgagg tcaacgggtt gcgcgtggtg ctcgcccgat
     2761 tcatacacga cgtcgagcac attccgatcc ccgtggggat agatgcgatt gacgcgggcg
     2821 cgaatctcgt tgatgcggac gttttccggc gcgccccggc gaatctcccc aaagaaccac
     2881 cggagatacc agtcaacgat ccgatgcatc ggaagacggt gcgatccgga gctccgcatc
     2941 tccgtctggt ccgggaagtc gggcggatcg aaccggatcg tcccgctgcg cacgctcccg
     3001 atccactcgg cgagcgaggg tccggtgacg actggcccct gcagcagaca ggatgcgtca
     3061 ctgaacatcg tgatctcttc cgcgcgccgg ccgagcgtcg cgatgagcga ccagtcatcg
     3121 cgcatcggat gttccactcc cgccggggac gtgtcgtaca tcaagacgac aaccgatgtc
     3181 cggggagcaa cgagctgcgc gttcgcggtg atgcgttcga tcaagcccgt cacccgtggc
     3241 ccaccaccaa tgaacgcgat cacgacctcg tcatcggaca cactcatgac gttctcgcct
     3301 gcctctcatc tattccctgg ccgcgccagt tccgcgggtc atcgcgaccg gcggaggtgg
     3361 cgtgagaccg gctgcgcaga tccctcgcca cctccagcca cgggcaccgg gcatcaggag
     3421 ccggatgcct cgttccagtt ggcgatgttc cagtactggt agtcgaaggg ggagaacccg
     3481 aagttgaccg cgttcaaccg cttcgcatag ccgacgggct ggccgacttc aatcaggggg
     3541 acgatcgcga cgtcgttgac cgcgatgtcg ttgagctgga tcagcaatgc cgccagcttc
     3601 tcgttgtcgg tctcgacccg tgcctgctcc agaagcgcgt cgaagtccgc gttctgccag
     3661 cgggagtagt tgccgcccga ccagctgttc gccttctggg cgatgttggc gccatccttt
     3721 cccgcgtaca ggaaattcag ccaggtgacc gggtaggcgc tcaggctggg tcgaccgtat
     3781 tcctgcaggt cccacgggaa gtgggtgatg ttctgcgtgt tgctcgccga gccgtcgaag
     3841 aactgccccg agtcgatctg gacgagctcg accttgaagc cgaccttttc caactgcgac
     3901 ttgatcagct gttgcgtctt ctggcgcacc tgattcaccg atgtcgagaa ggtgatgctg
     3961 agttccttgc catccttctt ccgcgtcgat ccgtcgagcg tccagcctgc ctcgtcgagg
     4021 agctggcctg ccttgtcggg atcgaaatcg agcttcgtgt tcggggaggt cacgtccttg
     4081 ccaccgagca cgatgttcga cgtcgcggga ttccctggct ggagatagaa ggtgctggcg
     4141 acctgctcgc gatcgatcgc ataggtcaat gcctgccgga ccttgaggtc ggagaagaag
     4201 gggtttggcg tgtccttctg cgagacctca ccgtcgacct ccttgcgcgg gtcggaatgg
     4261 ttgacgtcga tttcctccag cgaaatcgtg ttcgagacgt cgagcacgcc gggtccgcta
     4321 tcgctgacga agctctacag cacctctggt tagacctgga ggttccaggc gaagtcgtac
     4381 tcgcccgtct ggagaacggc gcgcgccgcg gatgccgcgt cgccaccacc cttgagcagg
     4441 actttcgcga aatagggctt gttcggctcg cggtagttct cgttggcgac gtaggtcacc
     4501 tggtcgttcg gcgagaagct gtcgacgacg aaaggaccgg tgccgatcgg cttgagcgcg
     4561 aaggcctcgg gcgtcggcag cgactcggtg tacgccttgg gcaacacgta cagatagccg
     4621 gccaccttgt tcaggaacgc gccgtccttg cgtgtgagct tgaacaccac ggtctgcaca
     4681 tcgggcgttt ccacgccttc gatcaaggtg gcgaagtagg gccggcgcgt gctcttggtc
     4741 tcgggcttca ggatgcgttc gatcgagaac ttcacgtcct gcgcgctgaa ggccgtgcc
//
LOCUS       DASTMD010000396         1721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_841178, whole genome shotgun sequence.
ACCESSION   DASTMD010000396 DASTMD010000000
VERSION     DASTMD010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841178"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VFQ54_05750"
     CDS             <1..548
                     /locus_tag="VFQ54_05750"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEU0164530.1"
                     /translation="ASREFRDSRLMTTPPSPDHFLPGPVHRGTSIRRLSMTAAIREQI
                     MLHLLAAAPNEGVGLLAVDAAVSDLGRATAARFFPGTNVDASPTRYTMDPVEVIQAVR
                     EMDARGWRWGAIVHSHLASPATPSQTDLREAYYPDVVLVIVTFQVHPAEMRAWRIDQE
                     DELRIVRGVDIEVVQPRRHGR"
     gene            825..1391
                     /locus_tag="VFQ54_05755"
     CDS             825..1391
                     /locus_tag="VFQ54_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0164531.1"
                     /translation="MHAALDASGGPGHPAGFGRDDDSEPTNGVPVVAFEGLRDRENIH
                     RALVAAVGDFERLIAGKSRETLAQPANDGRWGMVEILPHMRDWEKVNHDRIARVLNEE
                     NPHLEMPDDSLWAIEHDYAAQDPVRVFEEFKGLRGELVDLVEHLDDDAWSLPATLDGI
                     GEINLHWLLNNVCVHDGHHLQQARDLLV"
     gene            1482..>1721
                     /locus_tag="VFQ54_05760"
     CDS             1482..>1721
                     /locus_tag="VFQ54_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164532.1"
                     /translation="MASVSSFINVDAPVGADELRRIHALDNNALIVRLHFTINHLSRW
                     LSPIHDRRRLERSPYFAEPTVKDLLLAMRDEEHRVV"
BASE COUNT          311 a          531 c          556 g          323 t
ORIGIN      
        1 ccgcatccag agaattccgg gactcccgcc tgatgacgac accgccctcc ccagatcact
       61 tcctccccgg cccggttcat cgcgggacgt cgatccggcg tctctcgatg acggccgcga
      121 ttcgcgaaca gatcatgctt cacctgctcg cggcggcgcc caacgaggga gtcggcttgc
      181 ttgcggtcga cgccgccgtg agcgatctcg gccgggcgac ggcggcgcgc ttcttcccgg
      241 gcacgaacgt cgacgcgtcg ccgacccggt acacgatgga tccagtggaa gtgatccagg
      301 cggtgcggga aatggacgca cggggatggc gttggggagc gatcgttcac tctcatctcg
      361 ccagcccggc gacgccatcg cagacggatc tgcgcgaagc ctattacccg gacgtcgtgc
      421 tggtgatcgt cacctttcag gtccatccgg cggagatgcg ggcctggcga atcgatcagg
      481 aagatgagct gcgcatcgtc cgtggtgtcg atatcgaagt cgtccaacca cgtcgccacg
      541 gccgctgatc ggggagagtg gcccgcgagt tgcgttcagc tccacggtac cgggagacat
      601 gggtcctgtt ggtgctggcg gacggccagt atatagtttg tgccagtgtg tcacgagatt
      661 gaggtttgca aggttcctat ctgcttgcaa accggcggcg tgatgggacg gcgtccgatc
      721 tgctgggatc gggatcaacg cgcggatcac gccagcgtgg cacggggaat gagtggaaca
      781 gggagtcgat gaatctgctg ccctgcgtac ggaagcgtac gccgatgcat gcggccttgg
      841 atgcgtcggg tggaccgggt catcccgcgg gatttggccg cgacgatgat tcggagccaa
      901 ccaacggcgt gccggtcgtt gcattcgaag gactgcgcga ccgggagaac atccatcgcg
      961 cgctggttgc cgccgtcggt gacttcgagc gcctgattgc cgggaagtcg cgtgagacgc
     1021 tcgcgcagcc tgccaacgac ggacgctggg ggatggtcga gatcctgccc catatgcgcg
     1081 attgggagaa ggtcaatcac gaccgcatcg cgcgcgtact caacgaagag aatccccatc
     1141 tcgagatgcc ggatgattcg ctctgggcca tcgagcacga ctacgcggcg caggatcctg
     1201 ttcgcgtctt cgaggagttc aaggggctcc gcggcgaact cgtcgatctc gtcgaacacc
     1261 tcgatgatga cgcctggagt ctccccgcca cgcttgacgg cattggcgag atcaatctcc
     1321 actggctgct caacaacgtc tgcgtccacg acgggcatca cctgcaacag gctcgcgatc
     1381 tcctcgtctg agcgggcgca aagcgtgcca taattccctt ctacacggca tgcagggggt
     1441 catccatcat tggatgattc gcgtggacaa aggcggacgc gatggcgagt gtctcgagtt
     1501 tcatcaatgt cgatgcgcca gtcggggcag acgagctccg gcggattcac gcgctggaca
     1561 acaacgcgct gatcgtccgc ctgcatttca ccatcaatca cctttcgcgg tggctctcac
     1621 cgatccacga tcgccggcgt ctcgagcgat ccccgtactt tgccgaaccg acggtcaagg
     1681 atctgctgct cgcgatgcgc gacgaagagc accgcgtcgt t
//
LOCUS       DASTMD010000397         1558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_305136, whole genome shotgun sequence.
ACCESSION   DASTMD010000397 DASTMD010000000
VERSION     DASTMD010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305136"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1058)
                     /locus_tag="VFQ54_05765"
     CDS             complement(33..1058)
                     /locus_tag="VFQ54_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU0164533.1"
                     /translation="MVSYMNAPRFVGNGQIEWGKKEVPEPGPGQLLVQVRANALCGSD
                     RHQFYQGTDIVPGHEASGVVVATGSGTTIEIGTPGVIFLMDFCGECRNCRLGFTNLCL
                     AKRADMGFNRDGGYGQYMLIHENIFFPIDADLPFVEATMLLDIMGTGGHAINRARLVH
                     PDIQSVIVSGAGPIGLGVLAMAKTLLGVDVPVFITDFAPYRLELAARLGGLPIDLAKG
                     TLEDGLGTHGYERIDVAIDTSGKASARQASLAVVAKPGALVCVGHGEGLTLDVSRDLI
                     GTEHTVLGSEYFTFGELAGNLVHLRNDRDYFGQIITHRFGVDELQHAYELFFRGETGK
                     VVVQHDD"
     gene            1355..>1558
                     /locus_tag="VFQ54_05770"
     CDS             1355..>1558
                     /locus_tag="VFQ54_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164534.1"
                     /translation="MRLQRSGILTAVVSLVFIGSAIATSAAAATAQDAATPAPLGAES
                     CTVDPIDPTTYDAAIQKAIPPLPQ"
BASE COUNT          289 a          523 c          471 g          275 t
ORIGIN      
        1 gcggcgtcgt gccattcagc gagtggcacg actcagtcgt cgtgctgaac aacgaccttc
       61 cccgtctccc cacggaagaa gagctcgtac gcatgctgga gctcgtcgac gccgaaccgg
      121 tgcgtgatga tctggccgaa gtagtcacga tcgtttcgca ggtggacgag attgccggcg
      181 agttcgccga aggtgaagta ctcactacca agaaccgtgt gctcggtccc gatcagatcg
      241 cgtgagacgt cgagcgtcag cccctcgcca tgcccgacgc agaccagcgc tcccggtttc
      301 gccaccacgg cgaggcttgc ctgacgcgcc gatgccttgc cggaggtatc gatcgcgaca
      361 tcgatccgct cgtacccgtg cgtccccagt ccatcctcga gcgtgccttt tgccaggtcg
      421 atcgggagtc cacccagacg cgccgccagt tcgagccggt agggcgcaaa gtcggtgatg
      481 aagaccggca cgtcgacgcc gagcagcgtc ttcgccattg ccagcacgcc gagcccgatc
      541 ggtccggcgc cggagacgat cacggactgg atatcgggat gcaccagccg cgcccggttg
      601 atcgcgtggc cacccgtgcc catgatgtcg agcagcatcg tcgcttcgac gaagggcaga
      661 tcggcatcga tgggaaagaa gatgttctcg tggatgagca tgtactgccc gtacccgcca
      721 tcccggttga atcccatgtc ggcgcgcttc gccaggcaga ggttcgtgaa cccgagccgg
      781 cagttgcgac attccccgca gaagtccatc aggaagatca cgcccggcgt gccgatctcg
      841 atcgtggtcc cggatccggt cgcgacgacg accccggagg cctcgtggcc gggaacgata
      901 tccgtcccct ggtagaactg atgccggtcc gagccgcaga gtgcattggc cctgacctgc
      961 acgaggagtt gccccggacc gggctccggc acctccttct ttccccactc gatctggccg
     1021 ttcccgacaa agcggggagc gttcatgtag ctgaccacgg tgtcatcctt ctgcgagtca
     1081 attcggcgca agctggcgtc aagatgtggc gatcattcca actcgcggcc cgggtgtcaa
     1141 tcgcgatccg caccagtcca cgccgaatcg tcaggacccg ggacgacaac ccctgccctg
     1201 tcgcattgcc agcgcgatcc gtcccgatgc tgcgcatcat cttgacaggt ccttcgctca
     1261 tccctaggct atgcgacaag taacagacac atgtcgataa gtaacgagac gagcggggca
     1321 tgtccccgct cgacgagaga ttggagggac gaccatgcga ctgcaacgct ctgggatcct
     1381 gacggcggtt gtttcgcttg tattcatcgg atccgcgatc gcgacatccg cagccgctgc
     1441 caccgcccag gatgcggcga cgcccgctcc gctcggggcg gagagttgca ccgtcgatcc
     1501 tatcgatccg acgacctacg acgccgcgat ccagaaggcg atcccaccgc tgccacag
//
LOCUS       DASTMD010000398         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2983683, whole genome shotgun sequence.
ACCESSION   DASTMD010000398 DASTMD010000000
VERSION     DASTMD010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2983683"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2088
                     /locus_tag="VFQ54_05775"
     CDS             <1..2088
                     /locus_tag="VFQ54_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU0164535.1"
                     /translation="HEMEHPAELAGSGAGTMAGMSHGSDHAGHSNHAGHAGHGGHAEI
                     FRQRFWISLVLSIPVVLWSEMVQDWFNYSMPTFTSSDLISPVLGTIIFFYGGWVFLAG
                     GWDELKSRKPGMMLLISLAIVVAWAASLASSFDWVDLEFWWELALLVTVMLLGHWQEM
                     RAIGQAQGALDTLAALLPDEAERVSSGSETETVPVSALSLNDIVLIRPGGRVPADGVV
                     TDGRAELDESMITGESRPVSRTIGDRVVAGTVVAGSAIRVRVDAIGEATALAGIQRLV
                     ADAQNSRSNAQVLADRFAALLFYGALGSGAITFIIWSIVGSIDDAISRTVTVLVISCP
                     HALGLAIPLVIAISTAMAARQGILVRDRLALERMRLVDTVLFDKTGTLTEGAHAVREI
                     RSVAPDLDESGLLGLAASVEADSEHPLAKAIVTAARDRALAIPPAHDFSAIAGRGVEA
                     MVGGASIAIGGPGMLRERKLMVPEALVAEETVWEGRGAAVLHVVRNGEVVGALELEDR
                     VRPEAKEAVDELHQARVRVVMITGDAKSVAQAVGDEIGVDEVFAEVLPEDKLSAIATL
                     QKRGAKVAMVGDGVNDAPALAQADAGIAIGAGTDVAIASAGVILATSDPRAVMGIRRL
                     SQASYRKSIQNLVWAAGYNIIAIPLAAGVLAPVGFVLDPAVGALMMSLSTIIVALNAQ
                     LLRRVDLRPGAGK"
     gene            2139..>2441
                     /locus_tag="VFQ54_05780"
     CDS             2139..>2441
                     /locus_tag="VFQ54_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164536.1"
                     /translation="MSRMMSRIYRWPRGLAFATVALVLLAGTPSVSAQDHDMTMGTPS
                     ATATPAGGWSCGTAGTPAAMNHDEMGCMNSMTMIYEFDQLYIDMMLPHHGSIIALAE"
BASE COUNT          377 a          754 c          875 g          435 t
ORIGIN      
        1 cacgagatgg agcacccggc ggagctcgcg ggaagcggcg ccggcacgat ggccgggatg
       61 tcgcatggaa gcgaccacgc cgggcattcc aatcatgccg gacacgcggg acatggcggc
      121 cacgccgaga tcttccggca acggttctgg atcagcctcg tcctctcgat tccggtcgtg
      181 ctctggagcg agatggtcca ggactggttc aactactcga tgccgacctt caccagcagc
      241 gatctgatct caccggtcct cggcacgatc atcttcttct acgggggatg ggtcttcctc
      301 gcgggtggct gggatgagct gaaatcccgc aagccgggga tgatgttgct gatctcgctg
      361 gcgatcgtcg tcgcctgggc ggcgagtctc gccagcagct tcgactgggt cgatctcgag
      421 ttctggtggg aactcgccct tctcgtcacc gtgatgctgc tcggtcactg gcaggagatg
      481 cgcgcgatcg ggcaggccca gggggcgctc gatacgctcg cggcactgtt gccggacgag
      541 gcggaacgcg tcagttccgg cagtgagacg gaaacggttc cggtctctgc cctttccctc
      601 aacgacatcg tcctcatccg gcccggagga cgcgttcccg ccgacggggt cgtcaccgac
      661 ggtcgcgccg agctcgacga gtcgatgatc acgggcgagt cgcgtccggt ctcgcggacg
      721 atcggcgatc gcgtcgtcgc gggaaccgtc gtcgccggct ccgcgatccg cgtccgggtt
      781 gatgcgatcg gtgaggcgac tgcgctcgcg ggcatccagc ggctcgtcgc cgatgcccag
      841 aactcgcgct ccaacgccca ggtgctggcc gatcgcttcg cggcgctcct cttctatggc
      901 gcgcttggct ccggggcgat caccttcatc atctggtcga tcgtcgggag tatcgacgac
      961 gcgatctcgc ggacggtgac cgtgctcgtg atttcctgcc cccacgcgct cggcctcgcg
     1021 attccgctcg tgatcgcgat ctcgacggcg atggcggcgc gccaggggat cctcgtccgc
     1081 gatcggctcg cgctggagcg gatgcgcctc gtcgataccg tgctcttcga caagacgggc
     1141 accctgaccg agggcgcgca cgcggtgcgc gagatccggt cggtcgctcc ggatctcgac
     1201 gagagcggcc tcctcgggct ggcggcttcg gtcgaggccg atagcgagca tcccctggcg
     1261 aaggcgatcg tgacggcggc gcgcgatcgt gcgctcgcga ttccccctgc gcatgacttc
     1321 tccgcgatcg ccgggcgtgg ggtcgaggct atggttggtg gggcgtcgat cgcgatcggg
     1381 ggacccggca tgcttcgcga acggaagctg atggtcccgg aagcgctcgt cgccgaggaa
     1441 acggtctggg agggacgtgg cgcggccgtg cttcatgtcg tgcggaacgg cgaggtcgtc
     1501 ggtgcgctcg aactcgagga cagggttcgt ccggaggcga aggaggccgt cgacgaattg
     1561 catcaggcga gagttcgcgt cgtgatgatc actggcgacg cgaagtcggt cgcccaggcg
     1621 gttggtgacg agatcggtgt cgacgaggtc tttgccgagg tcctgccgga ggacaagctg
     1681 tcggcgatcg cgacgctgca gaagcggggc gcgaaggtgg cgatggttgg cgatggcgtc
     1741 aacgatgcgc cggcgctcgc ccaggcggat gccgggatcg cgatcggcgc cggcacggat
     1801 gtcgcgatcg cctcggccgg cgtgatcctc gcgacgagcg atccccgtgc ggtgatgggg
     1861 attcgacgtc tgtcgcaggc cagctaccgg aaatccattc agaatctggt atgggcggca
     1921 gggtacaaca tcatcgcgat cccgctcgcg gcgggtgtcc tggcgccggt cgggttcgta
     1981 ctcgacccgg cggtgggagc gttgatgatg agcctgtcga cgatcatcgt cgccttgaat
     2041 gcacagttgc tgcgccgggt cgatctgcga ccgggcgcgg ggaaatgaca cgccggttcg
     2101 aagcgatcca tgtcgcgaac gaggagagga aacggccaat gtcgaggatg atgtcacgga
     2161 tttatcgatg gccgcgtggg ctggcgttcg ccaccgtcgc gctcgtgctg ctcgccggta
     2221 cgccgtcggt ctccgcccag gatcacgaca tgacgatggg gacgccatcg gcgacggcga
     2281 cacccgctgg cggatggtcg tgcgggacgg cgggaacgcc ggcggcgatg aatcacgatg
     2341 agatggggtg catgaactcg atgacgatga tctacgagtt cgaccagctc tacatcgaca
     2401 tgatgctgcc ccaccacggg agcatcatcg cactcgcgga a
//
LOCUS       DASTMD010000399         1627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_125958, whole genome shotgun sequence.
ACCESSION   DASTMD010000399 DASTMD010000000
VERSION     DASTMD010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125958"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VFQ54_05785"
     CDS             <1..803
                     /locus_tag="VFQ54_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0164537.1"
                     /translation="APEQVTNQLFSYLEGYLFATTCPPGDVAAIFVESVQSDGGDLVP
                     PDDFLPRLRDLCDRHGIYLVVDDVKVGLGRTGRFFSYEHAGITADLVILGKALGGGLP
                     LSALVGRAEILDAGTSTALFTTVGSATCCAAGLATIEAIESQGLIEGAAVKGTYLHDR
                     LVATLEDIPEVAEIRGLGLIQGIELVTDPATRAPNQPLAAAVVYRAWELGLILYYAGN
                     WSNVLELTPPLIITRDEIDSGVAILGQAIADARAGRVDPEKVAAYAGW"
     gene            complement(895..>1627)
                     /locus_tag="VFQ54_05790"
     CDS             complement(895..>1627)
                     /locus_tag="VFQ54_05790"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0164538.1"
                     /translation="DAYATFGDLIDAFRARLDEEAKKPGLVFYKSVIAYLTGLAVGTP
                     TEGEAINAWKARPGFRDPQEKILRDFLFRETALKAIEHDKPFQIHTGHTGVGQPWRNA
                     NPIELTSFFNSPGMDDVRFVLVHGGYPFNTEAGHMTVAYPNVFLDFSLMIPWSSIGIA
                     RRIEMTLEQAPTSKLMYGSDGIVAPEMYWISAKNGKRALGQVLDGLLAQDVIDEADGI
                     EIAHDILHRTAKRVYGVDITSTEIA"
BASE COUNT          274 a          520 c          542 g          291 t
ORIGIN      
        1 gcgcccctga gcaagtcacg aaccagctct tctcctatct cgaggggtat ctcttcgcga
       61 cgacctgtcc gccaggcgat gtcgccgcga ttttcgtcga atcggtgcag agcgacggtg
      121 gcgatctcgt gccgcctgac gacttcctgc cccgcctgcg cgatctctgc gaccggcacg
      181 gcatctacct ggtggtcgac gatgtcaaag tcggactcgg gcggaccgga cgcttcttct
      241 cctacgagca cgcgggaatc accgcagacc tcgtgatcct cggcaaggcg ctgggcggcg
      301 gtcttcccct gagcgcgctc gtcggacgcg ccgagatcct cgatgcggga acgagcaccg
      361 cgctcttcac gacagtcggc agcgcgacgt gctgcgcggc cggcctggcg acgatcgagg
      421 cgatcgagtc ccaggggttg atcgagggcg ccgccgtgaa aggcacgtat ctccacgacc
      481 ggctcgtcgc gacgctggag gacattcccg aggtcgccga gattcgtggg ctcggcctga
      541 tccagggtat cgagctggtc accgatccgg cgacgcgagc tccgaaccag ccgctcgccg
      601 cggcggtcgt ctatcgcgcc tgggaactcg gcctgatcct ctattacgcc gggaactggt
      661 cgaacgtgct ggaactgacg ccaccactga tcatcacccg tgacgagatc gactcaggcg
      721 tcgcgatcct cggccaggcg atcgccgatg cccgcgcggg ccgggtcgac cccgagaaag
      781 tcgccgccta cgcggggtgg tgagtcaggg caacgaattc gcgccgctcg cgttcgccgg
      841 cggcgcgatg tggtgtgcaa gggcgagatc tgatgaacca ggaacaggtg atcgttacgc
      901 gatctcagtc gaggtgatgt cgacgccata cacgcgtttc gcggtgcgat gcaggatgtc
      961 gtgcgcgatc tcgatgccgt ccgcctcgtc aatgacgtcc tgggcgagga gtccgtcgag
     1021 cacctggcca agcgcgcgct ttccgttctt cgcgctgatc cagtacatct ccggcgcgac
     1081 gatcccgtcc gagccgtaca tcagtttcga tgtcggcgcc tgctccagcg tcatctcgat
     1141 ccggcgggcg ataccgatcg atgaccaggg aatcatcagc gagaagtcga ggaagacatt
     1201 cggataggcg acggtcatgt gaccggcttc cgtattgaac gggtagccgc cgtggacgag
     1261 cacgaagcgg acgtcgtcca tgcccggcga attgaagaac gaggtcaact cgatgggatt
     1321 cgcgttccgc cagggctgac cgacgccggt gtgaccggtg tggatctgga acggcttgtc
     1381 gtgctcgatc gccttcagcg cagtctcgcg aaagaggaag tcgcgcagga tcttctcctg
     1441 cgggtcgcgg aagccgggac gcgccttcca ggcattgatc gcctcgccct cggtcggtgt
     1501 gccgaccgcg agaccggtca ggtaggcgat gacggacttg tagaagacga gccccggctt
     1561 cttcgcctcc tcgtcgagcc gcgcgcggaa ggcgtcgatc aggtcgccga aagtcgcgta
     1621 ggcgtcg
//
LOCUS       DASTMD010000400         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1288290, whole genome shotgun sequence.
ACCESSION   DASTMD010000400 DASTMD010000000
VERSION     DASTMD010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288290"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1387
                     /gene="radA"
                     /locus_tag="VFQ54_05795"
     CDS             23..1387
                     /gene="radA"
                     /locus_tag="VFQ54_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU0164539.1"
                     /translation="MPKVRTSWICQQCGAVSPGYLGKCPSCGQWHTMVETIEERPGGS
                     TRAASKSGRATMQSLAQIAPEGQSRMTIPIEELNRVLGGGLVPGSLVLVGGDPGIGKS
                     TLILQAAGVLGSAANPVVYVSAEESAQQIKLRADRLGVVSENLLVLSETDLDGILATV
                     ETVDPALVIVDSIQTVMLGEITSAAGSVSQVRECTSRLMQWAKPRNVPVFIIGHVTKE
                     GTIAGPRVLEHMVDAVLYLEGDRFHQYRILRAVKNRFGSTDEVGVFEMADIGLREVTN
                     PSEAFLEERSDVAAGSTVAVTMEGTRPILVEVQALATPSAFGLPRRSANGLDTSRLQL
                     LVAVLQKRVGLGLGTQDVYANVVGGLRVTEPAVDLSIAVALASSFRDRPVERSTVVIG
                     EIGLSGELRSVNQLDRRVNEAARLGFRRVVVPARSTSTSDLVHGVEVVKCGSAGEAIE
                     AALN"
     gene            complement(1394..>2471)
                     /locus_tag="VFQ54_05800"
     CDS             complement(1394..>2471)
                     /locus_tag="VFQ54_05800"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU0164540.1"
                     /translation="AVAVIQELRSRGTDVDIRWIGSHAGVEREIAESQGIPFQAIQTG
                     KLRRYVSPRTVIDAFRVPVGITQAWGHIRRFQPHVIYSTGGAVSVPTVIAGSRRAPIL
                     THEQTAQIGIANRISARFADVFAVTFHDTATLAQQIHKQVVITGNPVRTSLTGGTKEG
                     GYAHFGFSPELPVLYVTGGARGASPINTRLEPVLARLLATMQIVHQVGPASANDDAAR
                     LRALRATWPEETQRRYVVEEFVGSEIADLYAITDLVLGRAGAGTVTELSFMGFPSVLI
                     PLPGTWGDEQRKNAKILTGVGAAVTLEQEEATPERLAQTLESLIGDPARLQSMSDAAR
                     TRSQPDAASLLVDQLLQLAATARR"
BASE COUNT          400 a          741 c          888 g          442 t
ORIGIN      
        1 gttttcggat ttgtgaggcg tcatgccaaa ggtacggacg agctggattt gccagcaatg
       61 cggtgccgtc agccctggct atctcggcaa gtgtccgagt tgcgggcagt ggcacacgat
      121 ggtcgaaacg atcgaggagc ggcccggcgg ttcgacccgg gcagcgtcta agtcgggacg
      181 agcgacgatg cagtctctcg cgcagatcgc tcccgagggg cagtcgcgga tgacgattcc
      241 gatcgaggaa ctgaatcgcg tcctgggtgg tggactcgtg ccgggctcac tggttctcgt
      301 cggcggtgat ccggggatcg gcaagtcgac cctgatcctg caggcggcgg gagtactcgg
      361 ttcggcggcg aaccctgtcg tctacgtctc ggcggaggaa tcggcgcagc agatcaagct
      421 ccgggcggac cgcctcggcg tcgtctcgga gaatctcctc gtgctctcgg agacggatct
      481 ggatggaatc ctggcgactg tcgagaccgt cgacccggca ctggtgatcg tggactcgat
      541 ccagaccgtg atgctcggcg agatcacctc ggcggcgggg agtgtttccc aggttcgcga
      601 gtgcacatcc cggctgatgc agtgggcgaa gccgcggaac gtcccggtct tcatcatcgg
      661 acacgtgacc aaggaaggga cgatcgccgg accgcgcgtg ctcgagcata tggtcgacgc
      721 cgtgctctat ctggaaggcg atcgctttca ccagtaccgg attctccggg cggtgaagaa
      781 tcgcttcgga tcgacggacg aggtcggcgt gttcgagatg gctgatatcg gtctgcgcga
      841 ggtgacgaat ccctcggaag catttctcga ggagcgctcc gacgtggcgg ccggctcgac
      901 cgtggccgtg acgatggagg gcacgcggcc gattcttgtc gaggtgcagg ccctggcgac
      961 gccttccgcg ttcggtctgc cgcggcgcag tgcgaacggc ctggacacca gccggttaca
     1021 gctgctcgtc gccgtcctgc agaagcgggt cggtctcggc ctgggaacgc aggatgtcta
     1081 tgccaacgtc gtcggtggac tgagggtgac ggaaccggcg gtggatctct cgatcgctgt
     1141 cgcgctggcc tcgagcttcc gcgatcgccc cgtcgagcga tcgaccgtcg tgatcggcga
     1201 gatcggactc tcgggtgagt tacggtcggt caatcagctc gatcgccggg tcaacgaggc
     1261 agcgcgactt ggcttccgcc gtgtggtggt gccggctcgt tcgacgtcga cctcggatct
     1321 cgtccacggc gtggaggtcg tgaaatgcgg ctccgccggc gaggcgatcg aagccgcgct
     1381 gaactagggc atctcagcgg cgcgccgtcg cggcgagctg cagaagttgg tcgacgagga
     1441 gcgaggccgc gtccggctgc gaccgggtac gggcggcgtc ggacatcgat tgcagtctgg
     1501 ccggatcgcc gatcagcgat tcgagcgtct gggcaaggcg ctcgggtgtt gcctcttcct
     1561 gctcgagtgt gaccgccgcg ccgactccgg tgaggatctt cgcgttcttg cgctgctcgt
     1621 caccccaggt gcccggcagt ggaatgagga cggaggggaa tcccatgaac gagagctccg
     1681 tcaccgttcc ggccccggca cgcccgagta caagatccgt aatcgcgtaa aggtcggcga
     1741 tctcgctgcc aacgaactcc tcgacgacgt atcggcgttg cgtctcctcg ggccaggtgg
     1801 cgcggagtgc gcgcaggcgg gccgcgtcgt cgttggcgga ggcaggacca acctgatgga
     1861 cgatctgcat cgtcgccagc aggcgagcaa gcaccggttc gagacgcgtg ttgatcgggc
     1921 tcgcgccgcg agcgccgccc gtcacgtaga ggacgggaag ctccggcgag aagccgaaat
     1981 gcgcgtagcc gccttccttc gtcccgccgg tcagtgaggt ccgaaccggg ttgccggtga
     2041 tgacgacctg cttgtgaatc tgctgggcga gcgtcgccgt gtcatggaag gtcacggcga
     2101 agacatcggc gaagcgcgcg gagatccggt tcgcgatgcc gatctgggct gtctgctcgt
     2161 gggtgagaat cggtgcgcga cgagagccgg cgatgacggt cggcacactc acggcaccgc
     2221 cggtactgta gatcacatgg ggctggaagc gacgaatgtg accccaggcc tgggtgatgc
     2281 cgaccggcac ccggaatgca tcgatgaccg tgcggggaga gacgtagcgg cgaagctttc
     2341 cggtctgaat ggcctggaac gggatcccct gggactcggc gatctcgcgc tcgacgccgg
     2401 catgactgcc gatccaccgg atgtcgacgt ccgtgccgcg gcttcgcagt tcctggatca
     2461 cggcgaccgc c
//
LOCUS       DASTMD010000401         1818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2895601, whole genome shotgun sequence.
ACCESSION   DASTMD010000401 DASTMD010000000
VERSION     DASTMD010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2895601"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VFQ54_05805"
     CDS             <1..856
                     /locus_tag="VFQ54_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU0164541.1"
                     /translation="LQTIPNDGKTEVEVYPGTFGGVETPFAFAEGVVFAPIVNFSTTY
                     FATEIDAASLDFSKGTGELVALDGATGKILWDIQLPAPAYGAATVVNDVVFTAGLDAI
                     VRAFSTKDGSALWSWQAPAGVNAPLAASGDFLYVPAGGPIYASKDTKNPGKPSANLVA
                     LTIGGTATISGLATPESGAASPAAAATPAAAASGGTAFAVSTIDLVFDPKTLTIPANT
                     DVKITVTNKGVLQHDFTIDELKVHSKLLNGGDSDTVTINAAAGSYQYYCSVPGHKEAG
                     MVGTLTVK"
     gene            1132..1809
                     /locus_tag="VFQ54_05810"
     CDS             1132..1809
                     /locus_tag="VFQ54_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0164542.1"
                     /translation="MEDELRLSTLVRRGLTEEGHAVDVADTGEEALEWLHVASYDVMI
                     LDVMLPGIDGIEVCRRIRGRKNPIPILLLTAKDTVMDRVYGLDSGADDYLTKPFAFAE
                     LSARLRALNRRPREAVEPVLEAGDLRIDPARMQVWKRGEEITIANKEFRILEYLMRNP
                     NRVLTRAMIAEHVWDYDFPNLTNVIDVHIRSLRMKLDDPYPGALIQTVRGMGYRLHAP
                     AVMTTST"
BASE COUNT          330 a          581 c          573 g          334 t
ORIGIN      
        1 cctgcagacg attccgaacg atggcaagac ggaggtcgag gtgtacccag gtaccttcgg
       61 cggcgtcgag accccgttcg ccttcgccga gggtgtcgtc ttcgcgccga tcgtcaactt
      121 ctcgacgacc tatttcgcga ccgagatcga tgccgcatcg ctcgacttct cgaagggcac
      181 cggcgagctc gtcgccctcg acggcgcgac gggcaagatc ctctgggata tccagctgcc
      241 ggcaccggcc tatggtgcgg cgacggtcgt caatgacgtc gttttcaccg ccggtctcga
      301 cgcgattgtc cgcgccttct cgaccaagga tggtagcgct ctctggagct ggcaggcgcc
      361 ggctggcgtc aatgctccgc tcgcggcgtc aggcgatttc ctgtacgtcc cggctggcgg
      421 accgatctac gcgtccaagg acacgaagaa cccgggcaag ccgtccgcaa acctcgtcgc
      481 gctgacgatt ggcggcacgg cgacgatctc cggcctcgcg accccggaat cgggcgcagc
      541 cagcccggcc gcggcggcga ctccggcagc cgcagcctcg ggtggcacgg cgttcgcggt
      601 gagcacgatc gacctcgtgt tcgatccgaa gaccctgacg atcccggcca acaccgacgt
      661 caagatcacg gtgacgaaca agggcgtgct tcagcacgac ttcaccatcg atgagctcaa
      721 ggttcactcc aagctgctca acggtggcga ttccgacacg gtgacgatca acgccgccgc
      781 cggcagctac cagtactact gctcggttcc tggtcacaag gaagccggca tggtcgggac
      841 gctgacggtc aagtaaaccc gctcgttccc tccgccgtat cacgacggca acggaagtcc
      901 cccctggcct tcgggtcagg ggggattttc cttgccgatc ggggcatggt tcatgctggt
      961 gacgcgggaa ctgctcgaag acggcggcgc catattcgcc ggcagggtcc tcatatccgg
     1021 cagggtgaat cgacttcata cagatttcat cctgccggga tattctttcg cacgatgttt
     1081 cgtccgggtc cagtccagag agaaagcgac gaccccatga ggatcctgtt tgtggaggat
     1141 gaactccgcc tgtcgacgct ggttcgccgc ggactcacgg aggaaggcca tgccgtcgat
     1201 gtcgcggaca ccggcgagga agcgctggaa tggcttcatg tcgccagcta cgacgtgatg
     1261 atcctggatg tgatgctgcc cggcatcgac gggatcgagg tctgccggcg gatacgggga
     1321 aggaagaacc cgatcccgat cctgctgctg acggcgaagg atacggtgat ggatcgtgtc
     1381 tacgggctcg attccggcgc cgatgactac ctgacgaaac cgtttgcctt cgccgagctc
     1441 tcggcgcgtc tgcgtgcgct caaccggcgt ccgcgcgagg cggtcgagcc ggtcctcgag
     1501 gcgggggatc tccggatcga tccggcgcgg atgcaggtct ggaagcgtgg cgaggaaatc
     1561 accatcgcga acaaggagtt ccgcatcctc gagtacctca tgcgcaatcc gaatcgcgtt
     1621 ctcacccggg cgatgatcgc cgagcatgtg tgggactacg acttcccgaa cctgacgaat
     1681 gtcatcgacg tccacatccg ttcgctgcgg atgaaactcg atgatcccta tcctggtgca
     1741 ctgatccaga ccgtccgcgg gatggggtat cgcctgcacg cgcccgcggt catgaccacg
     1801 tccacatgat gcgtaact
//
LOCUS       DASTMD010000402         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1646261, whole genome shotgun sequence.
ACCESSION   DASTMD010000402 DASTMD010000000
VERSION     DASTMD010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646261"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VFQ54_05815"
     CDS             <1..813
                     /locus_tag="VFQ54_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164543.1"
                     /translation="GLDALIYDLYINSKMIIAGTPAGVSLSPEGGAHQSAVTISLGIE
                     LPNLISYEPAFGKELDWMLLEAIRQCCDRVNGKSTYLRLSTKPIDQKLMDEVLARLGE
                     AELRRQVLAGGYRLIDRTIATPDLPASDVVQIAVGGIMVPEAVDAARRLAEEGVAANV
                     LVITSPERLYDRVRTSRRAQMSDAHAPLDLGQLGTLIPASERHAPIVTVQDGASHSLA
                     FLGSVFGAPVVPLGVDEFGQSAARQALYQKMGIDADQIVNAALLALDLSTEV"
     gene            1128..2081
                     /locus_tag="VFQ54_05820"
     CDS             1128..2081
                     /locus_tag="VFQ54_05820"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEU0164544.1"
                     /translation="MRKLVLSVAALLASTIMSIWSIALPVSAAGARDRATPVPAGAAS
                     TQQSITVSGTKRTYLVYVPASAPASGAPLVVMMHGGGGSAAQAEKDYGWDQEADREGF
                     IVAYPDGLNHAWNVGGGCCGQPGTNGTDDVAFITAMVAAIETKSSVDPSRVYATGISN
                     GGMMSYRLACDTDLFAAIGPDSATMLGECPSPKPISVIHIHGTADTKIRYNGGPGTGT
                     PETIDGPAVPALNATWRKIDDCAAPTFITAGKVTTSIASCPDDRTVELISVAGAGHQW
                     PGGAPHPLRTILVDADPPSDALNATSTIWTFFAAHPKAASA"
BASE COUNT          364 a          705 c          675 g          359 t
ORIGIN      
        1 ggtctcgatg cgctcatcta cgatctctac atcaactcga agatgatcat cgccggaacg
       61 ccggcgggcg tctcgctctc gccggaaggc ggcgcccacc agagcgcggt gacgatttcg
      121 ctgggcatcg agctgccgaa cctgatcagc tacgagcccg ccttcggcaa ggagctcgac
      181 tggatgctcc tcgaggcgat ccgccagtgc tgcgaccgcg tgaacggcaa gtcgacctat
      241 ctccggctct cgacgaagcc gatcgatcag aagctgatgg atgaggtcct ggcgcgactg
      301 ggtgaagccg agctccgacg tcaggtcctg gccggaggct accgcctcat cgatcgcacc
      361 atcgcgacgc cggatctccc ggcgagcgat gtcgtccaga tcgcggtcgg cgggatcatg
      421 gtgccggaag ccgtcgacgc ggcgcgtcgg ttggcggagg aaggtgtcgc cgccaacgtc
      481 ctcgtgatca cgagtccgga gcgcctctac gaccgcgtcc gtacgtcgcg ccgggcccag
      541 atgtccgacg cccatgcgcc gctcgacctc ggccagctcg ggacgctgat cccggccagc
      601 gagcgccacg ctccgatcgt gaccgtccag gacggcgcat cccactcact cgcgttcctt
      661 ggcagcgtct tcggcgcgcc ggtcgtcccg ctcggtgtcg acgagttcgg tcaatccgcg
      721 gcccggcagg cgctctacca gaagatgggg atcgacgcgg accagatcgt caatgcggcg
      781 ctcctcgcgc tcgacctctc gaccgaagtc taggcgaagg cgggacatag aagtcccgcc
      841 ctacggacgt acgccgtcgc agagatcatc ctcgtagcgg gccacctcgt gtggcctatc
      901 gggcgcagcc cgatatgacc gttgcacgaa cgaagtgcgt cgaccacgat agctcgggaa
      961 ttggtcaacc ggccccctcc ccgtttcgct tccgggttta cgaggacatt acatccggtt
     1021 cggtaccgtg agcccagtcg tcgatcgatc ggaccgatcg cgacaggtgg caagtggtct
     1081 ggcgcgattc acacccacgg cgccggaagc gaaacggatc gaagcccatg cgaaaactcg
     1141 ttctctctgt cgccgcgctt ctcgcgtcaa cgattatgtc gatatggtcg atcgcgttgc
     1201 cggtctcggc tgccggggcg cgcgatcgcg cgacgcccgt tcccgctggt gcggcgtcga
     1261 cgcagcagtc gattaccgtc agtggtacga agcgaaccta tctcgtgtac gttcctgcct
     1321 cggcgccggc cagcggagcc ccacttgtcg tgatgatgca cggcggcggt ggatcggcag
     1381 cccaggccga gaaggactat ggatgggatc aggaagctga tcgggaggga ttcatcgtcg
     1441 cctaccccga cggtctcaac cacgcctgga atgtcggcgg tggctgttgc ggccagccgg
     1501 ggacgaacgg cacggacgac gttgccttca tcaccgcgat ggtcgcggca atcgagacga
     1561 agtcgagtgt cgatccgagc cgggtctatg cgaccggcat ctcgaacggc ggcatgatgt
     1621 cctaccgtct cgcgtgcgac accgatctct tcgcggcgat cggcccggat tcggcgacca
     1681 tgctgggaga atgcccgtcg ccgaaaccga tctccgtcat ccatatccac ggcacggcgg
     1741 atacgaagat tcgctacaac ggcggtccgg gcacgggaac gccggagaca atcgatggcc
     1801 cggcagtccc ggccctgaac gcgacgtggc gcaagatcga tgattgcgcg gccccgacat
     1861 tcatcaccgc aggcaaggtc acgacgtcga tcgccagttg cccggacgat cgcacggtcg
     1921 aactgatctc ggtcgcgggc gccggtcacc agtggccggg cggcgcacct cacccgttgc
     1981 ggacgatcct ggtggatgcc gatccgccgt ctgacgcctt gaacgcgaca tcgacgatct
     2041 ggaccttctt cgccgcgcat ccgaaggccg catcggcctg atgacgacgt gttcccgtcc
     2101 cac
//
LOCUS       DASTMD010000403         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2538551, whole genome shotgun sequence.
ACCESSION   DASTMD010000403 DASTMD010000000
VERSION     DASTMD010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2538551"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFQ54_05825"
     CDS             <1..274
                     /locus_tag="VFQ54_05825"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU0164545.1"
                     /translation="ERSIAVGLERSGRIITSAALIVVVVTASFVSADVVLIKALGLGI
                     ALAVALDATVVRALLVPATMKLLGSWNWWIPKKLDRALPRIFRPDH"
     gene            402..686
                     /locus_tag="VFQ54_05830"
     CDS             402..686
                     /locus_tag="VFQ54_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164546.1"
                     /translation="MPRPCVEVSSDEFVWKYGEESVTVTRIDSSWQVSYLSSGRLMGP
                     VQVVYQATHQLPKHAAWDVMSKVTNVAHDDEEGVETAMRVAQWMKSRPSA"
     gene            705..1574
                     /gene="mutM"
                     /locus_tag="VFQ54_05835"
     CDS             705..1574
                     /gene="mutM"
                     /locus_tag="VFQ54_05835"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU0164547.1"
                     /translation="MPELPEVETMRRNIERLTLGRAIVAVDLTLPKLLRDSPIPELTP
                     IVGQRFTTARRRAKILIVGISNDLSLLIHCKLAGQIAIFAPDGERAVAGHPVPSPVGP
                     YPHKSTHLRIDFEDGTILYYSDIRQFGWLRVMPSEDVDAAIAEFGFGPEAWGAPLDTD
                     ALDAVIQRRGIPIKTLLLDQSFIAGLGNIYVDETLFAACVHPARPARSLTADERARIL
                     EFIPLSLEEGVKQGGARIINSKAYPVDNFPAVHGREGEPCFRCGTPIQKIRVGQRGTY
                     FCPNCQLLDRAPA"
     gene            1920..>2710
                     /locus_tag="VFQ54_05840"
     CDS             1920..>2710
                     /locus_tag="VFQ54_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164548.1"
                     /translation="MPKNHSFEKSLWDQHLTRRGLAKVVTTAAGLAALAPALVSADDA
                     TPQSSPSPERPRGGTVTVASSSDWDTLDPHHSTDFSVWNQIFDPLVGVTKDLKYEGIL
                     AESWDISEDGLAYTFHLRKGITFHNGEPMNAAAVKFTYDRLMDPKTASTGAGFVPALK
                     ETVVVDEYTAKVVLSSPFSALLGNFTLSYFGILPPVATQQAGDDFGRNPVGTGPWKFK
                     EWIASDHIAPIRNDDYHNYHSYMENSGPVYPDQLVFTVISQAATQ"
BASE COUNT          510 a          881 c          790 g          529 t
ORIGIN      
        1 cgagcggagc atcgccgttg gcctcgaacg ctcggggcgg atcatcacga gcgccgcgct
       61 gatcgtcgtc gtcgtcacgg cttcgttcgt ctcggcggat gtcgtcctga tcaaggccct
      121 ggggctcggg attgcgctcg cggtcgcgct cgatgcgacc gtcgtccggg cgctgctcgt
      181 cccggcgacg atgaagcttt tgggttcctg gaactggtgg ataccgaaga agttggaccg
      241 agcgctgccc cggatctttc gccccgatca ttaaatgact gatatgcatc gagtgtacga
      301 gcacacgttg tcgtaacctt ggacggcgct ttgtatactg cgtgcctgtt cggcgtccat
      361 cccctgtgcc gcgacgattt gccacgggag tcttgctgca tatgccccgt ccctgcgttg
      421 aagtgagttc cgacgaattc gtgtggaagt acggcgaaga gagcgtcacc gtgaccagga
      481 tcgattcttc ctggcaggtt tcctacctct cctcgggtcg cctgatgggc cccgtccagg
      541 tcgtctatca ggccacccac cagcttccca agcacgctgc ctgggacgtg atgtccaagg
      601 tgacgaacgt cgctcacgac gacgaagagg gggtggagac cgcgatgcgc gtcgcgcagt
      661 ggatgaagag tcgcccctct gcctagaaac cacgagatcg ccgcgtgccc gagcttcccg
      721 aagtcgaaac gatgcgtcgc aacatcgagc gcctcacgct cgggcgagcg atcgttgccg
      781 tcgacctgac gctgccgaag ctgcttcggg attctcccat ccccgaactg acgccgatcg
      841 tggggcagcg tttcacgacg gcacgccgcc gggcaaagat cctgatcgtc gggatctcga
      901 acgacctgag cctgctgatc cactgcaagc tcgcgggcca gatcgcgatc ttcgctcctg
      961 acggcgagcg ggccgttgcc gggcatccgg tgccgtctcc ggtcggacca tatccccata
     1021 aatccacgca tctgcgaatc gacttcgagg acgggacgat tctctactac agcgacatcc
     1081 gccagtttgg ctggcttcgc gtgatgccct ccgaagatgt cgacgcggcg atcgccgagt
     1141 tcggcttcgg gcccgaggcc tggggagcgc cgctcgacac ggacgcgctc gacgccgtga
     1201 tccagcgacg cggaatcccg atcaagacgc tgctgctcga tcagtcgttc atcgccgggc
     1261 tgggcaacat ctacgtcgac gaaacgctct tcgccgcgtg cgttcacccg gcgcgtcccg
     1321 ccaggtcatt gacggcggat gagcgagcaa ggatcctcga gttcatcccc ctgtccctgg
     1381 aagagggcgt gaagcagggg ggggccagga tcatcaactc gaaggcctat ccggtcgaca
     1441 actttcccgc cgtccacgga cgcgaaggcg agccctgctt ccgttgcggg acgccgatcc
     1501 agaagattcg cgttggccag cgtggcactt atttctgccc gaactgccag ctcctcgacc
     1561 gggctcccgc ctgaccaacg ccaggtacat tgcgattggt tcaggctgtg ccgacctcaa
     1621 gaccagagca gagttcgtcc gcaggatcgt ggtctcccct cgttgattcc ccttgcttct
     1681 tatctgacgt tgtcactgag aacatgaaat aaacctgtca gactcaatga ttaaaactga
     1741 aatatgacgc acttatttaa cctttagcca acattgcggg caggcaggat ggctagtctg
     1801 aagccgttga cacggatgga accggtcgcg ccgacctgac catccgacgt cacacatggt
     1861 ccagcacgaa caacgcgcat cgaattgtca cttcctccag aaagggagct gctaaagtca
     1921 tgcccaaaaa tcactctttc gagaagtctc tttgggacca gcacctgacg cgccggggcc
     1981 tggccaaggt cgtcaccact gcggctggtc tcgccgccct cgctccggcc ctggtctctg
     2041 ccgacgatgc cacgccgcag tcgtctccgt cgcctgagcg tccgcgcgga ggaacggtca
     2101 cggttgcgtc ctcaagtgat tgggatacgc tcgatccgca tcactcgacg gacttctcgg
     2161 tctggaacca gatcttcgat cccctcgtcg gcgtcaccaa ggacctcaag tacgagggca
     2221 ttctcgccga atcgtgggac atctccgaag acgggctggc gtacaccttc catctccgaa
     2281 agggcatcac cttccacaac ggcgagccga tgaacgcagc cgccgtcaag ttcacctacg
     2341 atcgcctcat ggatcccaag acggcgtcca ccggggccgg gttcgttccg gcgctcaagg
     2401 agaccgtcgt ggtcgacgag tacacggcga aggtcgtcct gagctcgccg ttctccgcgc
     2461 tgctcggcaa cttcactctc tcctacttcg gcatcctgcc gccggtcgcg acccagcaag
     2521 cgggcgatga cttcggacga aacccggtcg gcaccggccc atggaagttc aaggagtgga
     2581 tcgcgagcga ccacatcgcg ccgattcgga acgacgacta tcacaactac cactcctaca
     2641 tggagaacag tggtcccgtg tatccggatc aactcgtctt taccgtgatc tcacaggcag
     2701 caacgcagat
//
LOCUS       DASTMD010000404         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2984168, whole genome shotgun sequence.
ACCESSION   DASTMD010000404 DASTMD010000000
VERSION     DASTMD010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2984168"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1075
                     /locus_tag="VFQ54_05845"
     CDS             341..1075
                     /locus_tag="VFQ54_05845"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEU0164549.1"
                     /translation="MDTAFTSVAVTRSYEHIVRQIHDAIRNGQLRRGEKLPTERVLSE
                     TFGVSRSVVREAVKVLVAQGIVVSRQGSGTFVLDSPAHFVSQTIMLAVPVEEESIGQL
                     IEFREVLDTLAAHRAAVRRTEEEALAIIDASKQSLAAAEANDLDAFGDADVRFHTLID
                     HAAANIYVAIASSSVRQAQGEMTRLFASDIGSFVHAAGQHSRIAEAILAGDPDAAGRA
                     MGEHVRYSGSITLKIPKEVQTPTATG"
     gene            1266..>2218
                     /locus_tag="VFQ54_05850"
     CDS             1266..>2218
                     /locus_tag="VFQ54_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164550.1"
                     /translation="MSHPTITRRRLVQGGVALSGLTALTASGLDQSVLASPATNPATR
                     QLAPIQSLSGKVVIGINGNPDDSVKKALGDAYKQRQPGVDISWETAERAADDYMSWLG
                     TQLATDPPGLDIVAGNYVSTYRGYVNLDQYRGTTNPYSNQVWDADLNWDLIRAVNGAG
                     ERIMLPTRSVHINWFYNKDLFTKAGVTAPPTTWAEFADVCAKLKASGVTPIGINFAWQ
                     LPQWFAEVYFDQYHIKWVDTVRAQDGDWNYDPDLDGSFSYDATDPNIHLKYTFSQQRF
                     YKAIQGGTLRFDTPEMTALVGNLAQIFPKYANEDIFVAGDV"
BASE COUNT          444 a          701 c          637 g          436 t
ORIGIN      
        1 ttccgtcgac tactccacga atgcgcttat cacgaactcg aaagggtcgt catcctgagc
       61 aacgctcgac ctgatcgagc cataaacgct gctcagtgtg cccccaactc tccggcgtac
      121 atcccgtgca cgttgctcac ggaaggctag gctcccgtga gcaacgtgca cgggatgtac
      181 gctcgactgt gcttcgcgcg gggtgacgac tcccttgcgt tcgtgctacg cgccacgtat
      241 cggtcgatgt gaccaatgtt cctgtccgct gtttacaaat tgtgtatcat gccgtatctt
      301 gatgatattg gtatgacaac ctgataaccg ggtaacttgc atggacacag ccttcacgtc
      361 ggttgcggtc acgcgatcgt acgagcacat cgtccgtcag attcatgatg cgattcgcaa
      421 cggtcagttg cggcgcggtg agaagctgcc gacggaacgc gtcctcagtg aaacctttgg
      481 cgtcagtcga tccgtggtgc gcgaggccgt caaggtcctc gtcgctcagg ggatcgtcgt
      541 gtcgcggcag gggagcggca ccttcgtgct ggattccccc gcacacttcg tctcgcaaac
      601 catcatgctc gctgtgccgg tggaagaaga atcgatcggc caactcatcg aatttcgcga
      661 ggtgcttgac acgctcgctg cccaccgcgc ggcggttcgg cgaacggagg aagaggcgct
      721 cgcgatcatt gatgcctcca aacagagtct cgccgccgcc gaggcgaacg acctcgacgc
      781 cttcggcgat gccgatgttc gcttccatac gttgatcgac catgctgccg cgaatatcta
      841 cgtcgcgatc gccagcagtt ccgttcgcca ggcgcagggg gaaatgaccc gcctcttcgc
      901 ttcggacatc gggtcgttcg ttcacgccgc cggtcaacat tcccggatcg ccgaggcgat
      961 tctggccggg gatcccgacg cggccggtcg cgccatgggc gagcacgtcc gatattccgg
     1021 gtcgatcacg ctcaagattc cgaaggaggt tcagacccca accgccaccg gctgatgccg
     1081 gcgtatgttc ccgatcgacc acattgccat cgcgcacgaa tcttcggttg ctgtcgcgca
     1141 gccgaatagg agggagtttg tcgtcgttcg cggccccaac gggccgttgt gcgaccgatc
     1201 gatcgccgag aaacgggtcc caatgtgaag tcatcagtga accagtcaga ggagacgaaa
     1261 tcacgatgtc acatccaacc attacccgac gccgtctcgt tcagggaggc gtagctctca
     1321 gcggactcac cgccctcacc gcctccggac tcgaccagtc cgttctggca tcgccagcca
     1381 ccaatccggc gacacgtcag cttgctccga tccagtccct cagcggcaag gtcgtgatcg
     1441 ggatcaatgg caatcccgat gattcggtca aaaaggcgct cggcgatgcc tacaagcaac
     1501 gccaacccgg ggtcgatatc tcgtgggaga cggcggagcg cgccgccgat gactacatga
     1561 gctggcttgg gacgcagctg gcgaccgatc cacccggtct cgatatcgtc gccggcaatt
     1621 acgtcagcac ctatcgcggt tacgtcaatc tggatcagta ccgcggcacg accaatccct
     1681 attcgaacca ggtctgggat gccgatctga actgggatct cattcgtgcc gtcaacggcg
     1741 ccggcgagcg aatcatgctg ccgacccgca gcgtccacat caactggttc tacaacaagg
     1801 atctcttcac caaggccggg gttacggcgc caccaacgac gtgggcggaa ttcgctgatg
     1861 tctgcgcgaa gctgaaagcg tccggtgtga ccccgatcgg catcaacttc gcgtggcagt
     1921 tgccacagtg gtttgccgag gtctacttcg accagtacca catcaagtgg gtcgacacgg
     1981 tgcgggcgca ggatggcgac tggaactacg atcccgatct cgacggatcg ttcagctacg
     2041 acgcgaccga tcccaacatc catctcaagt acacgttcag tcagcaacgc ttctacaagg
     2101 cgatccaggg cggcacgctc cgcttcgaca cgccggagat gacggcgctc gtcgggaacc
     2161 tggcgcagat ctttccgaaa tatgccaacg aagacatctt cgtcgcaggc gatgtcta
//
LOCUS       DASTMD010000405         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2806476, whole genome shotgun sequence.
ACCESSION   DASTMD010000405 DASTMD010000000
VERSION     DASTMD010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2806476"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFQ54_05855"
     CDS             complement(<1..699)
                     /locus_tag="VFQ54_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164551.1"
                     /translation="MGPYLLRRVIAIIPVVFGVTIAVFAMMHVVPGDPAEIMLGDFQT
                     SPEQLAQLRSQLHLGDPLVEQYGRFVIDAAQGNLGTSIRTKRPVSDMIWENLPSTIEL
                     TLAGLCIAMAIGVTLGIAAAIRQNTWVDVGSMVFAGLGVSMPSFWLGIMLVFLFSLHL
                     RWLPAAGGGDLKHLVMPALALGLGASAILARLVRSTMIEVLNQDYIVTARAKGLRPRS
                     IIVRHALRNALTPVV"
     gene            complement(785..2407)
                     /locus_tag="VFQ54_05860"
     CDS             complement(785..2407)
                     /locus_tag="VFQ54_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164552.1"
                     /translation="MAKTGRSAIDRRKFIQLAGAAGVATVVGARVTPTRVFADTAASG
                     GELVVGLDAEPPTLDPQASPSVATYPVVSSVFESLLYQNDKHELKPWLAETWEVNADS
                     TAFTFHLRKDVVFHDGEPFNADTVKWNFDRVVDPNFQAGGALAALYGYTGSKVIDDYT
                     VEVQFSKPFAPFLTQAAGGSLGIVSPKAVQSLGSDFATKPVGSGPFKVDSYTAGDNVV
                     IVRNDDYKRQAPWSDRTGPALLDKVTFRFIPEASTRSVTVETGETNLINTITAQDLPR
                     FDGNDDFAVAKIPWGGVPRTLFLNFKLGPTDDVNVRKAVNLAIDRQSLVNTVFKGLAE
                     PSIGFLTKSLLDDPSLSFPYDPDQAKSLLDKAGWVKGDGDVRQKDGKKLTFVLNAVDL
                     GAGAPPETQFVQANLIDVGFDATIKLQARAPWYEDNYKYLTNGPIGFIRSGDFDAAFS
                     AFHSSLIGKNFNFSGVDLANIDDLLERGRQESDPTARRKTYLDLEHALQDISASAPLV
                     DEYAIWVGQKKVQGLTFNGACYPVFSDVSITN"
BASE COUNT          502 a          784 c          787 g          461 t
ORIGIN      
        1 cacgaccggt gtcagtgcat tccgcagcgc atggcggacg atgatcgagc ggggtcggag
       61 tcccttcgcg cgtgcggtaa cgatgtagtc ctggttgagc acctcgatca tcgtcgatcg
      121 caccagccgc gcgaggatcg ccgacgcacc gagcccgaga gccagggcag gcatcacaag
      181 gtgcttcagg tccccgccac cagcggccgg aagccagcgc aggtgcaggg agaagaggaa
      241 cacgagcatg atgccgagcc agaaacttgg catggagaca ccgagaccgg cgaagaccat
      301 cgacccgacg tcgacccacg tgttctgccg gatcgccgcc gcgattccca gcgtgacacc
      361 gatcgccatg gcgatacaga gccccgccag ggtcagctca atcgtcgatg gcaggttttc
      421 ccagatcatg tccgagaccg gacgcttcgt tcgtatcgac gtcccgaggt tgccttgagc
      481 agcgtcaatg acgaatcgtc catactgctc gacaagcgga tcccccaggt gaagctggga
      541 gcgcagctgc gcgagttgct cgggactcgt ctggaagtcc ccgagcatga tttcggccgg
      601 atcgcccggc acgacatgca tcatggcgaa gaccgcgatg gtcacgccaa acacgaccgg
      661 gatgattgcg atcacccgcc tcaagagata agggcccata ccgctccaga tcggtgcggc
      721 gggtcgctcc acccctcggc caaagccttg gcagcagacg cgtagcgacc cgcgctaccg
      781 gatgctagtt cgtaatcgag acgtcactga agaccgggta gcaggcaccg ttgaacgtga
      841 gcccctggac cttcttctgt cccacccaga tcgcgtattc gtcgaccagg ggagccgagg
      901 ccgagatgtc ctgcagcgcg tgctcaagat cgagataggt cttgcgtcgc gccgtgggat
      961 cgctttcctg gcggccacgc tcgagcagat cgtcgatatt cgcgaggtcg acgcccgaga
     1021 aattgaagtt cttgccgatc agcgacgaat ggaacgccga gaacgcagcg tcgaagtccc
     1081 cggaccgaat gaacccgatc ggaccgttgg tcaggtactt gtagttgtcc tcgtaccacg
     1141 gggcacgggc ctgcaacttg atcgtggcgt cgaagccgac atcgatcaga ttggcctgga
     1201 cgaactgcgt ctcgggcgga gcgcctgccc ccagatccac cgcattgagg acgaaggtca
     1261 gcttcttgcc gtccttctgg cgaacgtctc cgtccccctt gacccaaccg gccttgtcca
     1321 ggagcgattt ggcctgatcc ggatcgtacg ggaaggagag agacgggtca tcgaggagtg
     1381 acttggtcag gaaaccaatc gagggctcgg cgagcccctt gaacacggtg ttcaccaggg
     1441 actggcgatc gatcgcgaga ttgaccgcct tgcggacgtt cacatcatcg gttggtccca
     1501 gcttgaagtt caggaacagc gtccgcggga caccgcccca ggggatcttg gcgacggcaa
     1561 agtcatcgtt cccatcgaac cgcggcaggt cctgagccgt gatcgtattg atcaggttcg
     1621 tttctccggt ttcgacggtg accgaccggg tgctcgcctc aggaatgaac cggaacgtca
     1681 ccttgtcgag aagcgcgggt ccggttcgat cgctccacgg cgcctggcgc ttgtagtcgt
     1741 cattgcggac gatgacgacg ttgtccccgg cggtgtagct gtcgaccttg aacgggcccg
     1801 aaccgaccgg tttggtcgca aagtcggaac cgagggactg gacggccttc ggtgagacga
     1861 tacccagcga cccaccggcg gcctgggtca ggaacggcgc aaacggtttg ctgaactgta
     1921 cttccaccgt gtaatcgtcg atgaccttgc ttccggtata gccatacaac gccgccaggg
     1981 cgccaccagc ctggaagttc gggtcgacga cgcggtcgaa gttccacttc accgtgtcgg
     2041 cattgaacgg ctcgccatcg tggaacacga catccttgcg caggtggaac gtgaatgccg
     2101 tgctatccgc attcacctcc caggtttccg ccagccacgg cttgagctca tgcttgtcgt
     2161 tctggtagag caggctctcg aacaccgagg agacgaccgg ataggtcgcc acactgggcg
     2221 acgcctgcgg gtcgagtgtc gggggctctg cgtcaagacc aacgaccagc tctcccccgg
     2281 atgctgccgt gtctgcgaat acccgagtcg gggtgactcg cgcacccacg acggtcgcta
     2341 ctccggcggc gccggcgagc tgtatgaact tacggcgatc gatcgcggaa cgtcctgtct
     2401 ttgccattac tggttgatcc ttccctaccg gaatgtggta aagccatagg cgtgcgaaac
     2461 cgcacgacga gaatgcggtg gccaccctgg tcactcgcgt aaagagcccg gtcatcgact
     2521 gtcttgaaat aaag
//
LOCUS       DASTMD010000406         3245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_306249, whole genome shotgun sequence.
ACCESSION   DASTMD010000406 DASTMD010000000
VERSION     DASTMD010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306249"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFQ54_05865"
     CDS             <1..331
                     /locus_tag="VFQ54_05865"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164553.1"
                     /translation="AIIPISCHSPMRTPMIVSEEVTMELVMTNDHEASLILDGRNVSE
                     LGYLDTVTIRRGEHTFQLVKIGSHNFYEAVRTKFNFQIRPDAIPTRSLQKNSTVRVRS
                     HATPDEA"
     gene            complement(656..1618)
                     /locus_tag="VFQ54_05870"
     CDS             complement(656..1618)
                     /locus_tag="VFQ54_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU0164554.1"
                     /translation="MDIEALVARGIQILLALGGAYLIATWFVLAIWTFRDIEARSRSV
                     ITQVFSTLLVVLFWVPGLMLYWLLRPKETLDEAYQRSLEEEYLLQDLEEMPLCPTCHH
                     FIEDEWQLCPHCNTQLRESCESCGHLIDLRWEICPYCGTDHHGHGHDHDERLVEVSEE
                     RWVDPAVIEKRLREAEYRRQREETKALPAVTADSADGRRELLGSLRRLDILRNQSAVN
                     GNGRHDHNHDDDDHVHDHSHDDDRVQNHEHDDVPIEDHDHQDERDDSVVAQNGSLNGT
                     DVAKAAPVEPPAPKGPNLADISDDPAPAKDSGDEETIPDRFKVR"
     gene            complement(1676..2014)
                     /locus_tag="VFQ54_05875"
     CDS             complement(1676..2014)
                     /locus_tag="VFQ54_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEU0164555.1"
                     /translation="MKLVIAVVQGTDAEELLKAVTARGFRATQINSTGGFLRESNVTL
                     MIGVQEEYVSEVRELVCQNCHSRTRFVNPLMPIIEPGEFYVPSPVEVLVGGATIFVVP
                     VERYERLGVA"
     gene            complement(2080..2703)
                     /gene="tmk"
                     /locus_tag="VFQ54_05880"
     CDS             complement(2080..2703)
                     /gene="tmk"
                     /locus_tag="VFQ54_05880"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU0164556.1"
                     /translation="MTGTFIVFEGPEGAGKSVQTSLLAALLRERGHSVVQTREPGGTE
                     IGDEVRTILLRQRDYAMLPETEVLLLAAARAQHVRTVILPALARGDVVICDRYVDSTY
                     AYQGGGRQMATESLVPIQEFATGGLRPDIRVLLDLDVEIGLGRRFANPATVNRIDNDD
                     IAFHQRVRATYLELVHAHPDDWLVVDASASVEKVSREIVAAILPRIE"
     gene            complement(2704..>3245)
                     /locus_tag="VFQ54_05885"
     CDS             complement(2704..>3245)
                     /locus_tag="VFQ54_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEU0164557.1"
                     /translation="LRIFPFAVSRNRLEATVQQLNVPVMLVREPSEADVVMTLKNAYR
                     QKPATLRDVEARGVPVYVLRSNTVTQMEHVLLSLFPRAGNSGSGSASASVMEADFAPV
                     RGGGSKAVRPDPVMIALTEAESAISAVMGGAPATALSPQSPPIRRMQHELAERYNLSS
                     RSRGRDPFRHVEIYRRGIQ"
BASE COUNT          592 a         1058 c         1001 g          594 t
ORIGIN      
        1 cgcgatcatc cccatcagct gtcactctcc gatgcgcacg ccgatgatcg tctccgagga
       61 agtgacgatg gaactcgtga tgacgaacga tcacgaggct tccctgatcc tggatggccg
      121 gaacgtctcc gagctcgggt atctcgacac cgtgacgatc cggcggggcg aacacacctt
      181 ccagctcgtg aagatcggct cccacaactt ctacgaggcc gtgcgcacga agttcaattt
      241 ccagatccgt cccgacgcga tcccgacacg atccctgcag aagaattcca ccgtccgggt
      301 ccggtcccac gccacccctg acgaggcctg aagccgtctc gcggcgagcc atgcaccgcc
      361 gctccccgca gaagccatcc tggcgtcgtc gattgggagc ggttcatagc gcgttcgcag
      421 gtcccattcc cggtgcatcc gccaaatcct gggcgcgatg gcgggaccga agaggcggca
      481 catagagatc ccgcccgacg aacggacgct ggccgtacgc cggtcgtccg tagggtggga
      541 cttcaatttc cccccttctg gggcttgggg acgccccatt tacccgccaa tgggaaacac
      601 ccggcgcaat tgcaccgggt gtttctccgc gttgaagtga ctcgggtatt cgcgatcagc
      661 ggaccttgaa gcggtccggg atcgtttcct cgtcgccact gtccttcgcg ggcgccggat
      721 cgtcgctgat gtcagccagg ttcggcccct tgggcgcggg cggctcgacg ggagcggcct
      781 ttgccacgtc ggttccgttc agcgacccgt tctgggcgac gacggagtcg tcgcgctcgt
      841 cctggtgatc gtggtcctcg ataggaacgt catcgtgctc gtgattctga acgcgatcgt
      901 cgtcgtggct gtggtcgtga acgtgatcgt cgtcgtcatg attgtggtcg tgacgaccgt
      961 tgccgttcac cgccgactgg tttcgcagga tatcgagccg gcgcagcgac ccgagcaact
     1021 cgcgccggcc atcggcggaa tccgccgtga cggccggcag tgccttcgtc tcctcgcgct
     1081 ggcggcggta ctctgcctcg cggaggcgct tctcgatcac ggcaggatcg acccagcgct
     1141 cctcggagac ctcgacaagc cgctcgtcat gatcgtggcc gtgaccgtgg tgatcggtgc
     1201 cacagtacgg gcagatttcc cagcgaaggt cgatcaggtg cccgcaactc tcgcaggact
     1261 cacggagttg ggtgttgcaa tgcgggcaca gctgccattc gtcctcgatg aagtgatggc
     1321 aggtcggaca cagcggcatc tcctcgagat cctgcaggag atactcctcc tcgagcgatc
     1381 gctggtaggc ctcgtcgagc gtttctttcg gacgcaggag ccaatagagc atcagtccgg
     1441 gcacccagaa caggacgacg agcaaggtcg agaagacctg ggtgatcacg cttcgactgc
     1501 gtgcctcgat gtcccggaag gtccagatcg ccagcacgaa ccaggtcgcg atgagatagg
     1561 ccccgcccag tgccagcagg atctgtatcc cgcgtgcgac gagcgcttcg atatccaaac
     1621 aactgctcct ttatggcccg cccgccgggt tcggaacgaa acaagggttc cgacgtcagg
     1681 ccaccccgag gcgttcgtac cgttcaaccg gaacgacgaa gatcgtcgcg ccgccaacca
     1741 gcacttccac cggactggga acatagaact cgccgggctc gatgatcggc atcaacggat
     1801 tgacgaaccg ggttcgcgaa tggcaattct gacaaacaag ttcccgaact tcagaaacgt
     1861 actcttcctg aacgccaatc atcagcgtca cgttactctc gcggagaaat ccgcccgtgc
     1921 tgttgatctg ggtagcccgg aatccgcgcg ccgtgacggc tttcagcaac tcctctgcat
     1981 cggttccctg tacgaccgca atgaccagtt tcatgggata tcgtccatag cgtcttcatc
     2041 gtcgggcccg ggacatcgtc gtcccggaca aaccatgtct cactctatcc ggggaagaat
     2101 cgccgcgaca atctctcgcg aaaccttttc gacggatgcg gacgcgtcga ccacgagcca
     2161 gtcatcggga tgagcgtgga cgagctcgag gtacgtcgca cgcacacgct ggtgaaaggc
     2221 gatgtcgtca ttgtcgatgc gattgactgt tgcggggttc gcgaatcgtc gtcccaggcc
     2281 gatctcgacg tccagatcga gcaggacacg gatgtccggc cgcaatccac cggtcgcaaa
     2341 ttcctggatc ggcaccaggg actccgtcgc catttgccgg ccgccaccct gatatgcata
     2401 cgtggaatcg acatagcggt cacagatgac cacgtcgcca cgcgcaagcg cggggaggat
     2461 gacggtgcga acgtgctggg cacgcgccgc ggcgagcaac aacacctccg tctccgggag
     2521 catagcatag tcccgttggc ggagaagaat ggtccggact tcatctccta tttccgtgcc
     2581 acctggctca cgcgtctgaa cgacggagtg gccacgctcc cggagcaagg cagcgagcag
     2641 ggaagtctgt acagacttcc ctgctccttc gggaccttcg aagacgatga aggttccggt
     2701 cacctactgg attcctcgcc ggtagatctc gacgtgccgg aacgggtcgc gcccgcggct
     2761 tcgcgacgag aggttgtagc gctcggcgag ctcgtgctgc atgcgccgga tcggcggcga
     2821 ctgcgggctc agcgcggtgg ccggcgcccc tcccatcacc gccgagatcg cgctctcggc
     2881 ctcggtgagg gcgatcatca cgggatccgg acgcacggcc ttgctgccgc cgcctcgaac
     2941 cggggcgaag tccgcctcca tcaccgaggc gctcgccgat ccgctgccgg aattccccgc
     3001 gcgtgggaag agcgagagca ggacatgctc catctgggtc accgtgttcg aacggaggac
     3061 gtagaccggc accccgcgcg cctcgacgtc gcgaagcgtc gccggcttct ggcggtacgc
     3121 gttcttgagc gtcatcacga catccgcctc cgacggctcg cggacgagca tcaccgggac
     3181 gttcagttgc tggacggtcg cctccaggcg gttccggctg accgcgaacg ggaagatccg
     3241 gagcg
//
LOCUS       DASTMD010000407         3916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1825789, whole genome shotgun sequence.
ACCESSION   DASTMD010000407 DASTMD010000000
VERSION     DASTMD010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825789"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFQ54_05890"
     CDS             <1..911
                     /locus_tag="VFQ54_05890"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEU0164558.1"
                     /translation="LFLCCHPSLSPPSQIALTLRAVGGLATAQIAHAFFVPEATMAQR
                     ISRAKQTIKASGSHFQMPPDTERDVRLRAVLHVLYLIFNEGYTSTSGPDLQSAELTNE
                     AIRLTREVHRLLPDDGEVTGLLALMLLTDARRAARTLADGSLVPLAEQDRSLWSPIAI
                     EEGVALITHALATSPLGPYQLQAAINAIHDEAPSAEETDWSEIVALYDLLARITPNPM
                     VTLNRAVAVAMARGPEAGLAALAPLDADQRMAKHHRLFAVRAHLQELAGDRVAARESY
                     RVAASRTTSLPEQRYLESRAARLKDD"
     gene            1002..1484
                     /locus_tag="VFQ54_05895"
     CDS             1002..1484
                     /locus_tag="VFQ54_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HEU0164559.1"
                     /translation="MTRLPTRIHGIVDYIAGIILIASPWLFGFNDHRTATLVGLLGGL
                     AIVILAGITMDELGIWKILPITSHLPLEALIGLALATSPLVLGFSGTAWIPFVIIGIG
                     LLLMDAFTERVAGGGIKRFVQVAHRERESPGEARRQFQRRLKERERLARHPQGPQRLT
                     "
     gene            complement(1748..3448)
                     /locus_tag="VFQ54_05900"
     CDS             complement(1748..3448)
                     /locus_tag="VFQ54_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164560.1"
                     /translation="MSNTAPATAQAVPAAPSFGESERWLALVTVLIGTFMILLDSTIV
                     NVAIPSIQSSLKASFEDIEWVVSGYALSYGLLLIPSGRLGDRFGHKTLFIIGLVGFTL
                     FSALCGTANSAEALVIWRVLQGAMAGVMNPQITAVIQIAFPPKERGRAFGIYGSVIGV
                     ATAAGPLVGGILIAANIRGMDWEPIFLINIPIGIVALIMSIRSLKQTYGRAGDLDYVG
                     VFLVSAAVLLLTIPLVEGRTLGWPAWTYISMVGSIVVFALFALWERRRIQLDLPPLVD
                     VRLFRNRAFTAGMGIALAYFGGFVGIFFVASLLIQSGFQHSALYAGLCMMPFSLGSLI
                     SASQSDKVAKRLGRNCLTIGATLVMIGLAGLIVTMHYAGTGLNGWELAPWFLVAGLGS
                     GLVIAPNVTLVLAGVPRQDAGSASGVLSATQRIGQAIGVCLVGIVLFGVLQSNANSSA
                     GSVTTDLHNDLVAAQMPAAQIDGAVANFDSCFEKRASASDPNAVPAGCEATDTTASDP
                     ASKAFAKAGKHALADNFNTASQQALAVSLALVAVTFLLIFFLPRRRDEGGQPAAPPSE
                     "
     gene            complement(3530..>3916)
                     /locus_tag="VFQ54_05905"
     CDS             complement(3530..>3916)
                     /locus_tag="VFQ54_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164561.1"
                     /translation="RTEQEGNRPERTVYQITDKGRTEMISLRDAILRDARLKPDPIDL
                     AIQFLRDELTVEEATMDLAIRKHALQDQLASWKVAQVDAAPHINDMERMVFVHAIRRL
                     ELELAWHEELLERLPELIASIPADDF"
BASE COUNT          713 a         1320 c         1233 g          650 t
ORIGIN      
        1 tcctctttct ctgctgccac ccgtcgctct cgccgccatc gcagatcgcc ctgaccctgc
       61 gcgccgtcgg cggtcttgcc accgcccaga tcgcccacgc cttcttcgtg ccggaagcga
      121 cgatggccca acgcatcagc cgggcaaagc agacgatcaa ggcgagcggc agccacttcc
      181 agatgccgcc cgatacggag cgggatgtcc gtctccgcgc cgttctccac gtcctctacc
      241 tgatcttcaa cgaggggtac acctcgacct cgggcccgga tctccagagc gccgaactca
      301 cgaacgaggc gatccgcctg acgcgtgaag tccatcgcct gctgccggac gacggggagg
      361 tgaccggtct cctcgcgctg atgctgttga cggatgcccg ccgcgcggca cggacactgg
      421 cggatggatc cctcgtgccg ctcgccgagc aggaccggag tctgtggagc ccgatcgcga
      481 tcgaggaagg cgtcgccctg atcacccatg cactggccac ctcaccgctg ggcccgtatc
      541 agctgcaggc ggcgatcaat gcgatccacg atgaggctcc gagcgcggag gagacggact
      601 ggtcggagat cgtcgccctc tacgatcttc tcgcgcggat cacgcccaac ccgatggtca
      661 ccctcaatcg cgcggtcgcg gtggcgatgg cacgcggtcc ggaggcgggt ctcgcggcgc
      721 tcgctccgct cgatgccgat cagcgcatgg cgaaacacca ccggctcttc gctgtccgtg
      781 cccacctcca ggaactcgcc ggagaccgtg tcgccgcccg cgagagctat cgggtcgctg
      841 ccagcagaac gacgagtctt cccgagcagc gctatctcga atcccgtgcc gcgcgcctga
      901 aggacgactg aagcaccccc gcgacgcggt gcccccatca tcccggcatc caatctgcga
      961 gggaccacaa gggagctgtt catcgcagca aggaggtccc catgacacgt ctcccgaccc
     1021 gaattcatgg catcgtggac tacattgccg gcatcatcct gatcgccagc ccctggctct
     1081 tcggattcaa cgaccatcgc acggcgacat tggtgggact gctcggcgga ctggcgatcg
     1141 tcatcctcgc cgggatcacc atggacgagc ttggcatctg gaagattctc cccataacga
     1201 gccacctgcc cctcgaagcc ctcatcggtc ttgccctcgc gacgtcgccc ctcgtcctcg
     1261 gcttctccgg tacggcctgg attccattcg tgatcatcgg catcggtctg ttgctcatgg
     1321 atgccttcac tgaacgcgtc gccggtggcg gcatcaagcg cttcgtgcag gtcgcgcatc
     1381 gcgaacgcga gagtcccggc gaggcccgtc gccagttcca gcgtcgtctc aaggagcggg
     1441 aacgcctggc gcggcatccg caagggccgc aacgactcac gtagttgtcg ctgggtgtga
     1501 ccacagaatc tcggaatata cgcgtgcctc tcccgagtcc gcttcgcggc ggggtatgaa
     1561 accccgccct accaaccccg gcagcaccaa atgtatcggc ggctccggac ttcgctgtag
     1621 tattcgccct gatctcccca tggtcgatct cgaccgtgac aaacgtggat ccccgtcccc
     1681 gcggcccatg tggccgcttc cccaaccgtg gcacccgggg ctccttgccg caacgggaca
     1741 gtggtcccta ctcgctggga ggagcggcgg gctggccacc ctcgtcccgc cgtcgcggca
     1801 ggaagaagat cagcaggaag gtcaccgcga cgagcgccag cgaaacagcc aacgcctgct
     1861 gtgaggcggt attgaagttg tcggcgaggg cgtgcttccc cgccttcgcg aaggccttgc
     1921 tggccggatc gctcgccgtg gtatcggtcg cctcgcaacc ggcgggcacc gcgttcggat
     1981 ccgacgcact cgctcgcttc tcgaagcaac tgtcgaaatt ggcgacggcc ccgtcgatct
     2041 gggccgccgg catctgggcg gccaccagat cgttgtggag atcggtcgtc acgctgcctg
     2101 ccgaggagtt cgcgttcgat tgcagcacgc cgaagaggac gatgccgacg agacagacgc
     2161 cgatcgcctg tccgatacgc tgcgtcgcgc tcagcacgcc actcgccgac ccggcatcct
     2221 gccgcggaac gcccgccagc accaacgtca cgttcggcgc gatgacgaga ccggagccca
     2281 gtcccgcgac caggaaccat ggggcgagtt cccagccatt gagtcccgtt cccgcgtagt
     2341 gcatcgtcac gatcagtccg gcgaggccga tcatcaccag tgtcgcgccg atcgtcaggc
     2401 agttccgccc gagtcgcttc gcgaccttgt ccgactgcga cgcgctgatc agcgaaccga
     2461 gggagaacgg catcatgcag agaccggcgt agagcgccga gtgctggaat ccgctctgga
     2521 tcagcagtga cgcgacgaag aagatgccga cgaagcctcc gaagtaggcc agcgcgatgc
     2581 ccatgcccgc ggtgaaggcg cgattccgga agaggcgaac atcgacgagt gggggcaggt
     2641 ccagctgaat ccggcgccgt tcccagaggg cgaagagcgc gaagaccacg atcgaaccga
     2701 ccatcgagat gtaggtccac gccggccagc cgagagtgcg tccctcgacg agcgggatcg
     2761 tcagcagcag cacggcggcg ctgacgagga agacacccac gtagtcgaga tcaccggcgc
     2821 ggccataggt ctgcttcagc gaacggatcg acatgatgag cgcgacgata ccgatcggga
     2881 tattgatcag gaagatcggc tcccaatcca tgccgcggat attcgcggcg atcaggatgc
     2941 cgccgacgag cggacccgcc gccgtcgcga cgccgatcac ggagccgtag atgccgaagg
     3001 ccctgccgcg ttccttcggc gggaaggcga tctggatcac cgccgtgatc tgcggattca
     3061 tcacgcccgc catcgcgccc tgcaggacgc gccagatcac gagcgcctcg gcggaattcg
     3121 cggtgccgca gagcgccgag aagagcgtga acccgaccag cccgatgatg aagagcgtct
     3181 tgtggccgaa gcggtcgccc aaccgtcccg atggaatcag gagcaagccg taggagaggg
     3241 cgtagccact gacgacccac tcgatgtcct cgaagctggc cttgagcgag gactggatcg
     3301 agggaatcgc gacattcacg atcgtgctgt cgagcaggat catgaatgtc ccgatcagga
     3361 cggtgacgag cgcaagccag cgctccgatt ctccgaacga tggtgcggca ggaactgcct
     3421 gagccgtcgc gggtgccgta ttggacatgt cgatttctcc gaacgtggaa acgatgggcg
     3481 catgaacgac gcggtcgaac gtctgtcgac cccgcgatca gttcatgcgt cagaaatcat
     3541 cggcgggtat cgaggcgatg agctcgggca gacgttcgag caattcctca tgccaggcaa
     3601 gctcgagctc gaggcggcga atcgcatgga cgaaaaccat gcgctccatg tcgttgatat
     3661 ggggggcggc gtcgacctgc gcgaccttcc agctggcgag ttgatcctgg agggcatgtt
     3721 tccggatcgc aaggtccatc gttgcctcct ccacggtgag ctcgtcccgc aggaactgga
     3781 tcgcgagatc gatcggatcg ggtttgagtc gcgcgtcacg caggatcgcg tcgcggaggg
     3841 agatcatctc cgttcgcccc ttgtccgtga tctggtagac cgtgcgttcc gggcgattcc
     3901 cctcctgctc ggtccg
//
LOCUS       DASTMD010000408         1960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2716809, whole genome shotgun sequence.
ACCESSION   DASTMD010000408 DASTMD010000000
VERSION     DASTMD010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2716809"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VFQ54_05910"
     CDS             complement(<1..944)
                     /locus_tag="VFQ54_05910"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU0164562.1"
                     /translation="MSSASQSRSGPLVRWARVAAHHPWRVIGGWVVALVVLVALSQAI
                     GGTFVDSFSVPGAESQKALDALNSRFPSAAGDSAQVVFKADTNITTDPAVQKQINGFI
                     AAAEKLPEVVSVSNPLAANSGNVSPNGQIAYLSVQYDKGSQDIETSAVDQLEDLVKAT
                     NGNGLETEVGGGMIGGTPGAASSELIGILAAVVVLLFMFGSLIAMGLPIITAGSGVVI
                     STLIVTIVANFIDMPSISTALLSMMGLGVGIDYSLLLITRFREERSNGRRSEDSIVTA
                     VDTAGRSVLFAGTVVVIALLGLWVTGVPFIGFLGLGGAV"
     gene            1174..1803
                     /locus_tag="VFQ54_05915"
     CDS             1174..1803
                     /locus_tag="VFQ54_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0164563.1"
                     /translation="MGVMATSIGRKQRHTGSGPGKEALIEVAQRLFTERGYDHVSLQQ
                     IAEEAGMTKGAMYYYFQNKEEVFARVSARIVAGLRDSVIAAMTTADSFEESLILCLVT
                     AVESASGNLELWFADSARVLPAGAVRAAALEDFGIDGGAMILLPYFQAAAANGDITRL
                     SPETAAHIFEKLLELMVRQEIDDRSPGAPASDDVEQQARVLVDVFLHGV"
BASE COUNT          410 a          594 c          618 g          338 t
ORIGIN      
        1 acggcgccac caagaccgag gaagccgatg aagggcacgc cggtcaccca gagtccaagg
       61 agcgcgatca cgacgaccgt tccggcgaag agtaccgagc gtcccgccgt gtctaccgcg
      121 gtaacgatcg aatcctcact ccgccgaccg ttggatcgct cttcacggaa acgcgtgatc
      181 aacagcaacg agtagtcgat tccgacgcca agccccatca tggagagcag cgcggtcgag
      241 atcgacggca tatcgatgaa gttggcgacg atcgtcacga tcagtgtcga gatcacgacg
      301 ccgctgccgg cggtgatgat cggcagtccc atcgcgatca gcgacccgaa catgaacagc
      361 aacacgacga ccgcggcgag gataccgatg agctcggagc tcgccgcacc cggggtgcca
      421 ccgatcatcc caccgccgac ttcggtctcc agaccgttgc cgtttgtcgc cttgacgaga
      481 tcctccagct gatcgaccgc gctggtctcg atgtcctgcg accccttgtc gtattgcacc
      541 gacaggtagg cgatctgacc gttcggcgag acgttaccgg aattcgccgc caggggattg
      601 cttaccgaga cgacttccgg caacttctcc gccgcagcga tgaagccatt gatctgcttc
      661 tgcaccgcgg ggtcggtggt aatgttcgtg tccgccttga agacaacctg cgcgctgtca
      721 cccgccgccg acgggaagcg ggaattgagg gcgtcgagcg ccttctgcga ttccgcgcca
      781 gggaccgaga agctgtcgac gaacgtgccg ccgatagctt gcgacagcgc gacgagaacg
      841 acgagggcga cgacccaccc tccgatcacg cgccagggat gatgcgcggc gacgcgcgcc
      901 caacggacga gcgggccaga ccgcgactga gacgctgaag acacttggtg aacctttcga
      961 ctgggggaat gcgccacaga ggggagtctt ggccagggcg gattggcgct gtaaacgtgg
     1021 ccatcgtgca gatgccgatc gcaccacgag tccacatact aaacgactac tcagtaggcc
     1081 gcaaggtggt gtatctttgc gagcgacaca agatcacggc attctcgcga acgaagccac
     1141 gcgtgaagtg gcagctcaaa gacgagcagg ggaatgggcg tcatggcgac atcgataggt
     1201 cgaaaacagc ggcacactgg tagcggtccc ggcaaggagg cgctgatcga ggtggcccaa
     1261 cggttattca ccgaacgcgg gtatgaccac gtctctctcc agcagattgc ggaagaggcg
     1321 gggatgacaa agggcgcgat gtattactac ttccagaaca aggaagaggt cttcgcgcgt
     1381 gtttccgctc gcatcgttgc cggactcaga gacagcgtga tcgcggcgat gacgaccgcc
     1441 gactcgttcg aagagtctct catcctgtgt ctggttacag ctgtggaatc cgcttccggg
     1501 aatctggaac tctggttcgc cgattcagcc cgcgtactcc cggctggggc cgtcagagct
     1561 gcagcgctgg aagatttcgg gattgatggt ggtgcgatga tcctgcttcc ttacttccag
     1621 gcggcggcgg cgaacggcga tatcacgcgg ctctcgccag agacggcagc gcacatcttt
     1681 gagaagctcc tggaattgat ggtgcgtcag gagattgatg atagatcacc tggagctcca
     1741 gcgtccgacg acgtcgagca gcaagcccgg gtacttgtcg acgtcttcct ccacggcgtg
     1801 tgacgcggtg gggactgccc gacgcaggaa tccgcgccag gcaatccaga attcggccta
     1861 gagcaagggt cggaagacgt agctgccgct ccaccccttg gtcgtcatcc tgagcttgtc
     1921 gaaggatccc cgcgcgaagc gcatccggcg cagccggact
//
LOCUS       DASTMD010000409         4424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3073853, whole genome shotgun sequence.
ACCESSION   DASTMD010000409 DASTMD010000000
VERSION     DASTMD010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3073853"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..2041)
                     /locus_tag="VFQ54_05920"
     CDS             complement(44..2041)
                     /locus_tag="VFQ54_05920"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0164564.1"
                     /translation="MDSAEVGLTARELAALGIAGQGFDRIDIEDSRRIRLLAFVDASF
                     FSPFTIRSGKRYPIFTGDPQWFSDNIPCMTACPSHTDISRYIALIADGRYADSYELNR
                     EHNVFPGCLGRMCARPCEEACRRKEVDAPIGICYLKRVAADYRGETRRETPPAPNGKS
                     VAVVGAGVCGMTAGRQLARKGYSVDIYERFPVPGGVMWTGVPEWRLPRDVIAEEVAQI
                     TDLGVRIHYNVEVGKDVQLRQLSDENDAVVLAAGCQTPTGLGVPGEELKGVVSGLQFL
                     ADVALGQTDVWVGKSVVTVGGGFTSMDCVRTVLRLGSERSVMTYRRTIHEIPVDEIEL
                     EEAEVEGVEIQYMVTPTRVVGDEDGNVIGLELVKNELGEPDRSGRRRPEPVPGSEFVI
                     DCDMVIVAIGQRQDNRFLGDMFQNTDRRGVPMLDQNLKSEIDNVWAAGDYVINPTNFI
                     SSIGEGKRVADLIDQELRGALPKQKEMEITRIPTEYVSTPNSLLSEGVAEWTMTAMSR
                     RLVWGDDYAGVGRQVMPVLPAAERGIGTFDNTLESELGFTKPIGFEEAKRCLQCQLNI
                     FIDGNRCILCNSCGDVCPHKCIEMISPERIYSIDGDVELAEMARGELGPFAAAMVIDE
                     RSCIRCGLCVDWCPTECLTMDHFRLTPAETRESVDLAIIGN"
     gene            3045..>4424
                     /locus_tag="VFQ54_05925"
     CDS             3045..>4424
                     /locus_tag="VFQ54_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEU0164565.1"
                     /translation="MQTFELITLLLVAVVALVWLAERIKIPYPMLLMVGGLVLAVTPW
                     TPTIELEPEFVLGVFLPPILFQAAQNTSVRDFKLNFPAIGRLAFGLVFATTALVAVVA
                     HWVIPGIGWSAAFVLGAIVSPPDAVAATAIFQRLGAPRRIVTILEGESLINDASAIVI
                     YTFAVAAVKTGDFSPLDAVWSFVVVVVVGLAVGFVGGKVLGWVLPLLGDPTLVSIVTL
                     LTPFAVYVVGETLHGSGVLAVVVAGLVHGYESPRTMTATSRLRGIAIWDLVTIAINGL
                     VFILIGFELGALRETLSSTDVGRILWHGVVVVVAVVAARFAYILLLGGITKGGSRTGY
                     SPFTELADALDFKSQVLVAWSGLRGVVTMATALALPLVVDSGAAFDHRDEIILIAAFA
                     IVVTLFGFGLPLPWVLRKLELVDDGSYETEMDLAHKTIRSAMHTQITTVVRDDPEMAK
                     FFQPFLNEMQ"
BASE COUNT          701 a         1374 c         1482 g          867 t
ORIGIN      
        1 cgcgcatgac ttatgcgcgg gtgcatttct ctagggatcg aggctagttg ccgataatcg
       61 cgaggtcgac gctctcccgc gtctctgccg gggtgagccg gaagtggtcc atcgtcaggc
      121 actccgtcgg acaccagtcg acgcagagac cgcaacggat gcaggagcgc tcgtcgatca
      181 ccatcgccgc cgcgaacggt ccgagctcac cccgcgccat ctccgcgagt tcgacatcgc
      241 cgtcgatgct gtagatgcgt tccggcgaga tcatctcgat gcacttgtgg gggcagacat
      301 cgccgcagct gttgcagagg atgcagcggt tgccatcgat gaagatgttc agctggcact
      361 ggaggcagcg cttcgcctcc tcgaacccga tcggcttcgt gaagccgagc tcactctcga
      421 gcgtgttgtc gaaggtgccg attccccgtt ccgctgccgg aagcaccggc atcacctgac
      481 gaccgacgcc ggcgtagtcg tcgccccaga cgagacggcg actcatcgcg gtcatcgtcc
      541 actcggcgac gccctcgctc aggagcgagt tcggcgtgga gacgtactcg gtggggatgc
      601 gcgtgatctc catctccttc tgcttcggca gggcgccgcg cagttcctgg tcgatgagat
      661 ccgcgacgcg cttgccttcg ccgatcgagg agatgaagtt ggtcgggttg atcacgtagt
      721 cgccggctgc ccagacgttg tcgatctcgc tcttcaggtt ctggtcgagc atcggtacgc
      781 cgcgacgatc cgtgttctgg aacatgtcgc cgaggaagcg gttgtcctgc cgctggccga
      841 tcgcgacgat caccatgtcg cagtcgatca cgaactcgga gccggggaca ggttccgggc
      901 gacgacgtcc ggagcgatcc ggctcgccga gctcgttctt gaccagctcg aggccgatca
      961 cgttgccgtc ttcgtcaccg acgacgcggg tcggcgtcac catgtactgg atctcgacgc
     1021 cctcgacctc cgcctcttcg agttcgatct cgtcgacggg aatctcgtgg atcgtgcggc
     1081 ggtaggtcat caccgagcgc tcggaaccga gccggaggac ggtgcgaacg cagtccatcg
     1141 aggtgaaacc accgccgacc gtgacgacgc tcttgccgac ccagacgtcg gtctgaccga
     1201 gggcgacatc ggcgaggaac tggagtcccg agacgacgcc cttcagctcc tcgcccggga
     1261 cgccgagacc ggtcggcgtc tggcatccgg cggcgaggac gactgcatcg ttctcgtcgc
     1321 tgagttgccg gagctggacg tccttgccga cttcgacgtt gtagtggatg cgcacgccga
     1381 gatcggtgat ctgcgcgacc tcttccgcga tcacgtcgcg tggcaggcgc cattcgggga
     1441 cgccggtcca catcacgccg ccgggaaccg ggaagcgttc gtagatgtcg acggagtagc
     1501 ccttgcgcgc gagctggcga cccgccgtca tcccgcagac tccggcgccg acgacagcaa
     1561 ccgacttgcc gttcggagcg ggcggagtct cgcggcgcgt ctcgccacgg taatcggcgg
     1621 caacgcgctt gagatagcag atgccgatcg gggcatcgac ttccttgcgg cggcaggctt
     1681 cttcacaggg gcgggcgcac atgcgaccga ggcagccggg gaagacattg tgctcgcggt
     1741 tgagttcgta gctgtccgcg tagcgaccat ccgcgatcag cgcgatgtac cgcgagatat
     1801 cggtatggga tgggcaggcc gtcatgcagg ggatgttgtc ggagaaccac tggggatcgc
     1861 ccgtgaagat cgggtatcgc ttgccggagc ggatcgtgaa cggcgagaag aacgatgcgt
     1921 cgacgaaggc gaggaggcgg atgcgtcggc tgtcctcgat gtcgattcga tcgaatccct
     1981 ggccggcgat gccgagcgcg gcgagttcgc gggcggtgag cccgacctcg gcggagtcca
     2041 tcgcccggaa gcgatcggcg ggcgccccgc aggaaggaca ttcatcgggc ggcgctgccc
     2101 cttcgtgaat ccagccgcag actttacatc ggaacatggg tcgttctccg gtaatgtcat
     2161 tcgaactgtt ggcgccgtct cgtcatcgac ggctcttcac tttgacctga acgggcgaca
     2221 tcgccccttg ctgtagagat tcgtgaagta tagcggcttt cgacttgcaa gcgactcgtt
     2281 cctgagaatt cgttcaatag cggatgtcca gcggggagac cgggtacacc atctccgcac
     2341 tcagcgacca tcggggaacc gtagacgcgg cccgtgttgc cactacgggt cggcgtagcc
     2401 gatccgttcc ctggaagcgc attgtgtgcg gtgatggtgg ttgagtccgg ctgcgccgga
     2461 tgtgcttcgc acggggggtt ctcgacaggc tcgaagtgac attggagagg gggtcttcag
     2521 ggcgcatgtg gtgccgatgc gatccctcga caggctcggg aggacgtgtc ttggcggaca
     2581 ccgcatgcaa ggtctgctga ccggggcagc gcagtccatc acgctcgacg cctttctccc
     2641 attccgtcat cccgagcgga gcgacgacgc gccgccgtca ggcgcgcgac ggagcgcagt
     2701 cgagggaccg cgtcccattg gcgacgcctc gcctgtctgc tctccttccg accaacgcgt
     2761 ggtgcgggaa gcatgtggac gggaagcatg tggacgccaa ccggtccctc gacacgctgc
     2821 tctgaaaaac gcggcccttc aagccccatg acacccgctt ttcatgccgc gctggcgagg
     2881 cgaacgcttc atgactactc gggaggacgg tcatcggctg gtgcgctgtg gaattccagg
     2941 gcatcgcgcg ccggtgaaga taccgatcct ggcaaggtcg cggctatcat tcccggaggt
     3001 gatcgattgg aggagtgggg gcaggatggg agcggggaac gccggtgcaa acgtttgagc
     3061 tgatcacgct cctcctggtg gccgtcgtgg ccctggtctg gctcgcggag cggatcaaga
     3121 tcccatatcc gatgctcctg atggtcggtg gactcgtgct cgcggtgacg ccgtggacgc
     3181 cgacgatcga actcgagcca gagttcgtgc tcggcgtctt cctgccgccg atcctcttcc
     3241 aggcggcgca gaacacgtcc gtccgtgatt tcaagctgaa tttcccggcg atcggccggt
     3301 tggcgtttgg tctcgtcttt gccacgacgg cgctcgtcgc cgtggtggcg cactgggtca
     3361 tcccggggat cggctggtcg gcggcattcg tgctcggcgc gatcgtctcc ccgcccgatg
     3421 cggtcgccgc gacggcgatc tttcagcggc tcggcgcacc gcggcggatc gtgacgattc
     3481 tcgagggcga gagtctcatc aacgacgcga gcgcgatcgt catctatacc tttgccgtcg
     3541 ccgcggtgaa gaccggggac ttctcgccgc tcgacgcggt ctggagtttc gtggtcgtcg
     3601 tggtcgttgg tctcgccgtc ggtttcgtcg gtgggaaggt gctcggctgg gtactgccgc
     3661 ttctcggcga ccccaccctt gtatcgatcg tgacgcttct gaccccgttc gccgtctacg
     3721 tcgtaggcga gacgttgcac ggatccggtg tccttgccgt ggtggtggca gggctcgttc
     3781 acgggtacga atccccgcgg acgatgacgg cgacttctcg tcttcgcggg atcgcgatct
     3841 gggatctcgt gacgattgcc atcaacggac ttgtcttcat cctgatcggc ttcgaactcg
     3901 gcgcccttcg cgaaacgctc agctcgaccg acgtggggcg catcctctgg cacggcgtcg
     3961 tggtcgtggt cgccgtcgtg gcggcccgct tcgcctatat cctgctcctt ggcgggataa
     4021 cgaagggtgg cagtcgcacc ggctactcac cgttcacgga gctcgccgat gcgctggatt
     4081 tcaagtcgca ggtgctcgtt gcctggtccg gcctccgcgg tgtggtgacg atggcgacgg
     4141 cgctcgccct gccattggtg gtggactccg gcgcggcgtt cgatcatcgt gacgaaatca
     4201 ttctgattgc ggcattcgcg atcgtcgtga cgctcttcgg gttcgggctg cctctgccgt
     4261 gggtgctgcg caagctcgaa ctcgtcgacg atgggtcata cgagaccgag atggacctgg
     4321 cccacaagac gatccggtcg gcgatgcata cgcagataac gaccgtggta cgggacgacc
     4381 cggagatggc gaagttcttc cagcccttcc tcaacgagat gcag
//
LOCUS       DASTMD010000410         1722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3164711, whole genome shotgun sequence.
ACCESSION   DASTMD010000410 DASTMD010000000
VERSION     DASTMD010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3164711"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(416..1516)
                     /locus_tag="VFQ54_05930"
     CDS             complement(416..1516)
                     /locus_tag="VFQ54_05930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0164566.1"
                     /translation="MSEPPFTIEEIIIPATIDSADAGDFIATVEIRNAVEADIVGTDA
                     LAYAPVELLPRYLDQEFEPRKLFVARVDGKIVARAIYAWPIEPGDGERLADLAVEVLP
                     AFRGRGIGTALADHVEGLALAAGRTTWQPWVTHRTPRDADDALLSPTGFGALSASDPG
                     VRFLQRRGYRLEQVERISFLELPVSPGILAQHQQVAGAAAGSDYRIIRWSGSTPREWI
                     DDLVLLRTRMSTDMPSAGLDIVEQAWTAERIMREDELQLTSGRERLSVAALHIPTGRL
                     AGFSQLEVGSDRARPVMQQDTLVLSEHRGHRLGMVLKIANIQHLMEVNPRSTLLVTFN
                     AEENRHMLSVNEAVGFRPVGAEGAWKKVATTA"
BASE COUNT          310 a          577 c          526 g          309 t
ORIGIN      
        1 gacctcggca tccggaaggc gtgcatcgcg gcgaatccca taaacagatg acgatgcgat
       61 cgcatgacgc ccaagtcgtg attcgcagcg agagcgggcg aagatcgggg tctgtagggc
      121 gagagtttat ctctcgccgc gaagcggacg gtggagcaac atgtcctcgc ctacgcgatc
      181 atccgcgagg accgggttgg tagggcgggg tttcataccc cgccgcaaac ggaatctctc
      241 cgttcgcccg gctatcgccg ccgcgatctt tcgcccacgt gcttcgtgcc gtcagaggca
      301 ttggctgaac cgtcccgcct ggtgctggcc cgcggtacgt ccgcgccgca aacggcaact
      361 cgcctcgcaa caggccgtct ccgcgacagc cgtcacgaat tccacgacga ctcgctcacg
      421 ctgtcgtggc aaccttcttc caggcgccct cggcgccgac cgggcggaaa ccgactgcct
      481 cgttcacgct cagcatgtgg cggttctcct ccgcgttgaa cgtcacgagc aacgtcgacc
      541 gcggattcac ctccatcaga tgctggatgt tggcgatctt cagcaccatc ccgagccgat
      601 gaccgcgatg ctcggaaagc accagcgtat cctgctgcat caccggtctc gcgcgatcgc
      661 tcccgacctc aagctggctg aagccagcca ggcgccccgt tgggatatgc agcgcggcga
      721 ccgagagacg ctcgcgaccg ctcgtcaact gcaactcgtc ctcccgcatg atccgctcgg
      781 cagtccatgc ctgctcgacg atgtccagac ccgccgacgg catgtcggtg ctcatccggg
      841 tgcggaggag caccaggtca tcgatccact cccgtggtgt gctgccactc cagcggatga
      901 tcctgtaatc cgatccggct gcggctcccg ccacctgctg gtgctgggca aggatccccg
      961 gcgagaccgg cagctcgaga aagctgatcc gctcgacctg ctccagccgg tagccacggc
     1021 gttgcaggaa gcgcacgcca ggatcgctgg ccgagagcgc cccgaaaccg gtcggcgaga
     1081 ggagcgcatc atcagcatcc cgcggcgttc gatgcgtcac ccatggttgc cacgtcgttc
     1141 gaccggcggc gagcgccaac ccctcgacgt gatcggcaag cgccgtcccg atcccgcgac
     1201 cgcgaaacgc cggaaggacc tcgacggcaa gatcggcgag gcgctcgccg tcgcccggtt
     1261 cgatcggcca ggcgtagatc gctcgtgcca cgatcttccc gtcgacgcgc gccacgaaga
     1321 gcttccgtgg ttcaaattcc tgatcgaggt agcgcgggag cagctcgacc ggcgcatagg
     1381 caagcgcgtc cgtgccgacg atgtcggcct cgacggcgtt ccggatctcc accgtcgcga
     1441 tgaagtcacc ggcgtcggcg ctgtcgatcg tggcggggat gatgatttct tcaatcgtga
     1501 aaggtggttc ggacatcact attcctccca attcgcggtc gggcaattcg cttcggtcac
     1561 gatgatacga tgtcgataat tcaaactcga ttccgacttc tctgtgaggt gtccagaatg
     1621 ggcgccgccg ctgtgccggg agcctgccat gaaatatctg gtgctcgtct acgggaacaa
     1681 gaacttctgg aaatcattct cgccggagga attcgcggag at
//
LOCUS       DASTMD010000411         1918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_485981, whole genome shotgun sequence.
ACCESSION   DASTMD010000411 DASTMD010000000
VERSION     DASTMD010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485981"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="VFQ54_05935"
     CDS             complement(<1..1027)
                     /locus_tag="VFQ54_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164567.1"
                     /translation="MKDSSVDQLFKDAKAGKISRRTLVSSAAAVGASAALMGTAAAQA
                     TPDASPVASPGASPAASPAAYTGPVGTISVSREDFYKALNEKFQFTKADKTGGTLIQL
                     QTSDLTTLNPLLSSDTYSTQIIQLYNDTLVQTSPIDGTYVPSLADSWEIAADGRTYTF
                     HLHKGVTWSDGQPFTADDVVYSFDATVDEKSLSPRRADVLSVLESYRKIDDLTVELVA
                     QKPFATFLSKTVAEVGIAPKHIWESVSFDQWGADPGSTGTDASRVVGTGAFLFKEWVQ
                     NDHVTLEKNPNYWNAANVPVNIDEFIYRVVADSNAALTSFTSGEVDILQQVQPAQAEQ
                     LKKSNPEI"
     gene            complement(1403..>1918)
                     /locus_tag="VFQ54_05940"
     CDS             complement(1403..>1918)
                     /locus_tag="VFQ54_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164568.1"
                     /translation="GIVDKDGVKFSFECEYPEGIASYTQQLPYMQQAWKEIGVEMIPV
                     SIPFTTLVDHVTSSNYVMSVLGFSWGVDPDQGTMFRTDAKPPAGFNLTAYSNPKYDSL
                     NDQQNVELDVKKRVDLLIEQTNLVNEDLGTGVTVFQKSIAASAPRVHNYVSNAYVFGG
                     TWSYSYVWLDS"
BASE COUNT          350 a          554 c          620 g          394 t
ORIGIN      
        1 cgatctccgg attcgacttc ttcagctgtt ccgcctgcgc cggctgcacc tgctggagaa
       61 tgtccacctc acccgaggtg aacgaggtca gcgccgcgtt ggagtccgcg acgacgcggt
      121 agatgaactc gtcgatgttg acgggaacat tcgccgcgtt ccagtagttc gggttcttct
      181 ccagggtgac gtggtcgttc tggacccatt ccttgaagag gaacgcaccg gtgccgacaa
      241 cgcgtgacgc gtcggtaccg gtcgaacccg gatcggcgcc ccactgatcg aaggagaccg
      301 attcccagat gtgcttgggg gcgatgccga cttcggcgac cgtcttgctg aggaacgtcg
      361 cgaacggctt ctgggcgacg agctccaccg tcaggtcgtc gatcttcctg taactttcga
      421 ggaccgagag cacatccgcc cggcgcgggc tgaggctctt ctcatcaacc gtcgcgtcga
      481 aggagtagac gacgtcgtca gccgtgaacg gctggccgtc gctccacgtg acacccttgt
      541 ggagatggaa cgtgtaggtt cggccatcgg ccgcgatctc ccaggagtcc gcgagcgaag
      601 gcacgtaggt gccatcgatc gggctcgtct gcacgagcgt gtcgttgtag agctggatga
      661 tctgggtgga gtaggtgtcc gaggaaagga gcgggttcag tgtcgtcaga tccgatgtct
      721 gcagctggat cagcgtgccg ccggtcttgt cggccttcgt gaactggaac ttttcgttga
      781 gcgccttgta gaagtcctca cgggagaccg agatagtgcc gaccggcccg gtgtaggcag
      841 ccggagacgc cgcaggtgat gcgcccgggg atgcgacagg cgaggcatcc ggcgtcgcct
      901 gcgcggcggc ggtacccatg agggcggcgg aggctccgac agcggccgcg ctggagacga
      961 gcgtgcggcg gctgatcttg cctgctttgg cgtccttgaa caactgatcg accgaagaat
     1021 ccttcatgca tgaacctccc gggttcggtg atgtctttcc ctaaacaagc gccgtggcgc
     1081 ccacgatcca tcgcgagatg gatgctcgat gccgtcacac cacctttcga tgtcgctatg
     1141 aaatcccgtg gcctttgagg tacccagctg tgactatacc cggcattccg tttggaagtg
     1201 gcctcgccag agcatcatat gaaggtcttc gccaaatcgt caagaaaaaa gcggcaaaca
     1261 tggcagcgtt accttgggca aactgcacag gacgctcggc cgttttattt gttttgtgag
     1321 cataggcaac gagagactcc gttcccgcga ccgggaacgg agtctctcgt tggtgccgga
     1381 ttgtcgcccg atcaggcgaa tcctagctgt cgagccagac gtaggagtac gaccacgtcc
     1441 cgccgaagac ataggcgttg gagacgtagt tgtgcacgcg cggcgcggag gcggcgatcg
     1501 acttctggaa gaccgtgacg cccgtcccga gatcctcgtt gacgagattc gtctgctcga
     1561 tgaggagatc gacgcgcttc ttgacgtcga gctcgacatt ctgctgatcg ttcagcgagt
     1621 cgtacttggg gttgctgtag gccgtgaggt tgaagccggc aggtggcttc gcgtccgtgc
     1681 ggaacatcgt gccctggtcc ggatccacgc cccacgagaa gccgaggacc gacatcacgt
     1741 agttgctcga cgtgacgtga tcgacgagcg tcgtgaacgg gatcgagacc gggatcatct
     1801 cgacgccgat ttccttccat gcctgctgca tgtatggcag ctgctgcgtg taggaggcga
     1861 tgccttccgg gtattcgcac tcgaagctga acttgacgcc gtccttgtcg acgatgcc
//
LOCUS       DASTMD010000412         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2985297, whole genome shotgun sequence.
ACCESSION   DASTMD010000412 DASTMD010000000
VERSION     DASTMD010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2985297"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VFQ54_05945"
     CDS             <1..864
                     /locus_tag="VFQ54_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0164569.1"
                     /translation="EEWKPDLIVCDEMDFGAMIAAERFGIPHATVIVLTAGGFTRPDL
                     VAESLDEVRAEHGLPPDPDVVMPGRYLLIEPSPPSFRDPHHPLPAGARRIRPYVLEAE
                     AAANAPAWIEELDRADDPVIYFTLGTIFNLESGDLFTRMLDGLRELPARIVVTVGKEL
                     DPERFGPQPANVRIERFVPQATLLPHCDLAISHGGSGSITGALAFAVPQIVIPMGADQ
                     LHNAARVEALGVGVALDAVDLTPESVHATVLAMLANAPAREASVRMRDEILALPPSAA
                     AIPWLEALVRS"
     gene            complement(870..1667)
                     /locus_tag="VFQ54_05950"
     CDS             complement(870..1667)
                     /locus_tag="VFQ54_05950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0164570.1"
                     /translation="MAHDSMDWVDGFYSATGTWWGQAESAVTERDHRRLATMERLAGR
                     SGERLRVLELGSSYGNTAFVAAQAGHDVIGVELSDRIDFTRRFAIDEVAGNLQFVKAD
                     FYDFELEAPVDVVTYWNGFGIGSDADQRRLLRRMIECWLKPDGIVLMDVSSPARWIQW
                     AGDEEHKTARPNAGYPFNVSERTDYDPIGNRFIDTWWRTESPDEPVSQDLRCYAPADL
                     LLLLEGTGLRLRHAEVEGIALDLDGVATSGHPLWRNHEYLVELEPER"
     gene            complement(1701..>2750)
                     /locus_tag="VFQ54_05955"
     CDS             complement(1701..>2750)
                     /locus_tag="VFQ54_05955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU0164571.1"
                     /translation="DDFYYLSQCRYDEGRARILAGDRDGAIVAFRQSLNAWSHMVTPE
                     LPSAIVLARSSLESLTGQWTRATIDDLLTVLGRAKTEYKAVTLAVYAALAEAMEHFGD
                     LAAANTYLRENAKLQEAYWREVATARSHHAAKALQTIEAQRTAESERRQRRAIDEAMQ
                     RVVILNAQNERLVTQLQEQSVVLEQMAREDSLTNLANRRYFDEQLQRELVRSESFQRP
                     FSLAMVDIDDFKAINDRYTHIVGDEVLRTVARLMRMSSRVTDFVARYGGEEFAVIFTE
                     ADHDEADALTEKIRSSIERHSWDRLAEGLSVTISIGLVTVSGNIGATAVVARADEMLY
                     AAKRRGKNLTASGWL"
BASE COUNT          454 a          889 c          900 g          507 t
ORIGIN      
        1 gaggagtgga agccggacct catagtctgc gacgagatgg atttcggtgc gatgatcgcg
       61 gccgaacggt tcgggatccc ccatgcgacg gtgatcgtgc tcaccgccgg tggcttcaca
      121 cgaccggatc tcgtcgccga gtcgctcgat gaagtgcgag ccgagcacgg cttgccgcca
      181 gatcccgatg tcgtgatgcc cggccgctac ctgctgatcg aaccgtcgcc gccatcgttc
      241 cgcgatccgc atcatccgct gcccgcgggg gctcgccgga ttcgacccta cgtcctggag
      301 gcagaggcgg ccgcgaacgc gccggcgtgg atcgaagagc tcgatcgcgc ggacgatccg
      361 gtcatctact tcacgctcgg cacgatcttc aatctcgaat caggcgatct cttcacccgg
      421 atgctggatg gacttcgcga gcttccggcg cgaatcgtgg tcaccgttgg caaggaactg
      481 gatccggagc gattcgggcc gcagcccgcg aatgtcagga tcgagcggtt cgtcccacag
      541 gccacgctcc tgccgcactg cgacctggcg atttcgcatg gtggatcggg aagcatcacg
      601 ggcgcgcttg ccttcgccgt gccgcagatc gtgattccga tgggagccga tcagctccac
      661 aatgccgcgc gcgtcgaagc actcggcgtt ggcgtagcgc tagatgcggt cgacctcacg
      721 ccggaatccg tgcacgcgac cgtcctcgcg atgctcgcga acgcgccggc acgcgaggcg
      781 tcggtgcgga tgcgggacga gatcctggcc ctgccgccgt ctgctgccgc gatcccgtgg
      841 ctcgaagccc tcgtccgatc ctgacggggt cagcgctcgg gctcgagctc gaccaggtat
      901 tcatgattgc gccagagcgg gtggccggac gtggcgaccc catcgaggtc gagggcaatc
      961 ccctcgactt ccgcgtgccg gagtcgcagc cccgttccct cgagcaggag gaggaggtcg
     1021 gcaggcgcat agcagcgaag atcctggctg accggctcgt ctggtgattc agtccgccac
     1081 caggtgtcga tgaagcgatt gccgatcggg tcgtaatcgg tgcgttcgct gacattgaag
     1141 ggatagccgg cgttgggtcg ggccgtcttg tgctcctcgt cacctgccca ctgaatccag
     1201 cgggccggac tcgagacgtc catgaggacg atgccgtccg gcttgagcca gcattcgatc
     1261 atccgccgga ggagtcgtcg ctgatcggca tcggagccga tcccgaaacc gttccagtag
     1321 gtgacgacat cgacgggagc ctcgagctcg aagtcgtaga agtcggcttt cacgaactgg
     1381 aggttgcctg ccacctcgtc gatagcgaat cgcctggtga agtcgatccg gtcgctcagt
     1441 tcgactccga tcacatcgtg accggcctgt gccgcgacga aggccgtgtt gccgtaggac
     1501 gatcccagtt cgagaacgcg gaggcgctcg ccggaacggc ccgcgaggcg ctccatcgtc
     1561 gcgagacggc ggtgatcccg ctccgtcacc gcggattccg cctgccccca ccaggtgccg
     1621 gtcgccgaat agaacccgtc gacccaatcc atgctgtcat gagccattga tgaccttcgt
     1681 ctccgttgcg atgcgaccgt tcagagccaa cccgacgcgg tcagattctt gccgcgccgt
     1741 ttcgccgcgt agagcatctc gtccgctcgc gcgaccacgg cggtcgcgcc gatattgccg
     1801 gagaccgtga cgagtccgat gctgatcgtc accgagagcc cttccgccag ccggtcccat
     1861 gaatggcgct cgatgctcga gcggatcttc tcggtcaggg cgtccgcctc gtcatggtcg
     1921 gcctccgtga agatgacggc gaattcctcg ccgccgtagc gggcgacgaa atccgtgacg
     1981 cggctgctca tgcgcatcag acgggcgacc gtgcggagaa cctcgtcacc gacgatgtgg
     2041 gtatagcgat cgttgatcgc tttgaagtcg tcgatatcga ccatcgccag cgagaatggc
     2101 cgctggaaac tctccgatcg cacgagctca cgctggagct gctcgtcgaa gtagcgacga
     2161 ttggccaggt tcgtcagcga atcctcgcgc gccatctgct caaggacgac cgactgctcc
     2221 tggagttgcg tgaccaaccg ttcgttctgg gcattgagga tcaccacacg ctgcatcgct
     2281 tcgtcgatcg cccgtcgttg ccggcgttcg ctttcggccg tgcgctgggc ttcgatggtc
     2341 tggagcgctt tcgccgcgtg atgcgagcga gcggtggcga cctcccgcca gtacgcctcc
     2401 tggagcttcg cattttcccg gagataggta ttggcggcgg cgagatcgcc gaagtgctcc
     2461 atcgcctcgg cgagcgcggc gtagacggcg agcgtcacgg ccttgtattc cgttttggcg
     2521 cgcccgagca ccgtgagaag atcgtcgatg gtggcgcggg tccactgccc ggtcagtgac
     2581 tccaaagagg atctcgcgag gacgatcgcc gacggaagct ccggggtgac catgtgcgac
     2641 caggcgttga gcgactgccg gaaggcgacg atcgcgccgt cgcggtcccc ggcgaggatg
     2701 cgggcgcgcc cttcatcgta ccggcattgc gacaggtaat agaagtcgtc
//
LOCUS       DASTMD010000413         1647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_217969, whole genome shotgun sequence.
ACCESSION   DASTMD010000413 DASTMD010000000
VERSION     DASTMD010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217969"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VFQ54_05960"
     CDS             <1..772
                     /locus_tag="VFQ54_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0164572.1"
                     /translation="TVRPHLVDLVRRMKEFDGIKELSMTTNALLLGRMAGDLKEAGLD
                     RVNISIDTLDPVKFKMMTRGGRFDLVWQGIEAADAVGMTPIKLNTVVVKDQNDLQVAE
                     MARLTLERDWQVRFLEIMPMEGVGMVYDEGLVTSDQTQARLEQEFGPLEPVSADPSDP
                     ARVWRIPGAKGTIGFISPISQPFCETCNRVRLTSDGNLRLCLLRPDEVTLRDRMRMGQ
                     SDEDIMEHLTAGIWRKPWGHRVHEGDVATVRGMSQIGG"
     gene            795..1073
                     /locus_tag="VFQ54_05965"
     CDS             795..1073
                     /locus_tag="VFQ54_05965"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEU0164573.1"
                     /translation="MFFFVIEHFKNGDPLPVRERFQDKGRLVPDGVTYVGSWIDPKTA
                     TCYQVMEAEARDLIDEWSSRWSDIVDFEIFEVLSSPDYWAAIDEKYPR"
     gene            1211..>1647
                     /locus_tag="VFQ54_05970"
     CDS             1211..>1647
                     /locus_tag="VFQ54_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEU0164574.1"
                     /translation="MSTISVVERALDRQFKLAKHRTSIRTELMAGLTTFMVMAYIIAV
                     NPATLAFNGALTITQVATVTCLVAGVMSIAMGLYANRAIALAPGLGLNAVVAYTLVGN
                     MGLSFPEAMGVVVSEGIVITLLVLTGARRYILEAIPIELKKAI"
BASE COUNT          292 a          509 c          538 g          308 t
ORIGIN      
        1 gaccgtccgt ccccacctcg tcgacctcgt gcgacggatg aaggaattcg acgggatcaa
       61 ggaactctcg atgacgacca acgcgctcct gcttggtcgg atggcgggtg acctcaagga
      121 agctggtctc gaccgggtca acatcagcat cgacacgctc gaccccgtca agttcaagat
      181 gatgacccgc ggcggtcgct tcgacctcgt ctggcagggc atcgaggcgg cggacgcagt
      241 cgggatgacg ccgatcaagc tcaacaccgt cgtcgtcaag gaccagaacg acctccaggt
      301 cgccgagatg gcgcgcctga ccctggagcg ggactggcag gtccggttcc tcgagatcat
      361 gccgatggaa ggcgtcggca tggtctacga cgaggggctg gtgacgtcgg accagacgca
      421 ggcccgactc gagcaggagt tcggtcccct cgaaccggtc tccgccgacc cgtccgatcc
      481 ggcccgtgtc tggcgtattc ccggcgcgaa gggcacgatc ggattcatct cgccgatctc
      541 ccagccgttc tgcgagacct gcaatcgcgt tcgtctcacc tcggacggca atcttcgtct
      601 ctgccttctt cggccggacg aggtgacgct gcgcgaccgg atgcgcatgg gccagtccga
      661 cgaggacatc atggagcacc tcaccgctgg aatctggcgc aagccgtggg gacatcgcgt
      721 gcatgaaggc gacgtcgcga cggttcgcgg catgtcccag atcggcggct gatcttcgcg
      781 ggtgaggggc gctggtgttc ttcttcgtga tcgagcactt caagaatggc gatccgttgc
      841 cggtccgcga gcgcttccag gacaagggac gcctcgtccc ggatggcgtg acctacgtcg
      901 ggagctggat cgacccgaag acggcgacct gctaccaggt gatggaagcc gaggcccgcg
      961 acctgatcga tgaatggtca tctcgctggt ccgacatcgt tgacttcgag atcttcgagg
     1021 ttctttcatc ccccgactat tgggcggcga tcgacgagaa ataccctcgt tagcgacgca
     1081 caatcgacat tgccgaaagt tgacagcgcc gtcgtccccc gggacgacgg cgctgttgcg
     1141 tccccgcgat ttcatgcgac gatgcaatca ggttcggctg gcatccgttg cgcggaggag
     1201 gggtgtgggg atgagcacta tttcggtcgt cgagcgggca ctggaccgcc agttcaagct
     1261 ggcgaagcac cggacgtcga tccgaacgga gctgatggcc gggctgacga ccttcatggt
     1321 catggcctac atcatcgcgg tgaacccggc gacgcttgcc ttcaacggtg cattgacgat
     1381 cacgcaggtc gcgacggtga cgtgcctcgt cgcgggcgtg atgtcgatcg cgatgggtct
     1441 ctatgcgaac cgggcgatcg cgctggcgcc tggcctcggt ctgaatgccg tcgtcgccta
     1501 cacgctcgtc gggaacatgg gactgagctt cccggaagcg atgggtgttg tcgtctccga
     1561 gggcatcgtc atcaccctgc tcgtgctgac cggcgcccgc cgctacattc tcgaggcgat
     1621 tccgatcgag ctgaagaagg cgataag
//
LOCUS       DASTMD010000414         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3165192, whole genome shotgun sequence.
ACCESSION   DASTMD010000414 DASTMD010000000
VERSION     DASTMD010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3165192"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1107..1583
                     /locus_tag="VFQ54_05975"
     CDS             1107..1583
                     /locus_tag="VFQ54_05975"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0164575.1"
                     /translation="MAKRVTIDVEIVVADWDDPDGIALRAAQRADIDDRYGNSTHEPG
                     GAPSGAEISVFLIARDGDGIACGCGALRQLDAGSAEIKRMYVAPERRGAGVSTAILNA
                     LEQHARARHWTTLRLETGSLLPEAMRFYEREGFRRIPNYGIYIGAEDSICYEKHLV"
     gene            2012..3538
                     /locus_tag="VFQ54_05980"
     CDS             2012..3538
                     /locus_tag="VFQ54_05980"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU0164576.1"
                     /translation="MSDLWDNIKDGIPGNVVGAVILLIIGWIVARIVATVVTKAARRL
                     NLDSRLAGLMGRSSDTTQSDSAGILGKIAYYGILLFVLIAVFDLLDMPLVTQPLNDLL
                     SGIFAYIPRLIGAVILILLAWVLATILKTVVFEFLRGVNIDARLKRLQGQEGAIGEPV
                     VARGLSDVVFALVFLLFLPAILSALNLTGILEPVQELYDKFFAFIPNLIAAAAIIIIG
                     WFVARFVARLIASLLAAAGSDRFAERVGISSALGARNLSGTIGLLIYVLILVPLIIAG
                     LNALQLDSITTPASNMLNSILQFIPRAFGAAAILVISFVVGRIVAGLISSLLAGTAYD
                     SLLSRLGLGTLTDGTEEAGAGISLAPLTGQAILAVIMWFAAIAALETLQLFTVSELLT
                     SLLALAGHILLGVIIFVVGLLIARFAGRLIQGAVTEQSGLIRFGAQAAIMVLFGAMAL
                     RQMGVANDIINLAFGILLGAVGIAIAVAFGVGGREIAGRQLTRWVESTQRDRNPETES
                     "
BASE COUNT          664 a         1186 c         1091 g          718 t
ORIGIN      
        1 ggccacccgc ccaacgtgag cgcagaccgc cccgccggga cgcctggtaa ctgggcaaca
       61 acccgccttc cgcgagcgac cgtgggaacc cgtcccggcg ccccacgtgg ccacccgccc
      121 aacgtgagcg cagaccgccc cgccgggacg cctggtaact gggcaacaac ccgccttccg
      181 cgagcgaccg tgggaacccg tccccacggc ccttgtggcc gttaccccga cgttgatgcg
      241 acacgccatg cccgatacct gcccatctcg acctccccga cttgccgtgc gaaaatcgcg
      301 ccagggtctc tcaactcaac caattgctga cattcccaaa ggcacgagat gtcaaggcaa
      361 ttcatcccac gcgccgattg gcgcctaagg gcttacgact acacgctccc tggtccgtac
      421 ttcgtgaccc aggcacttag ccaaaggcac cccagattcg gcgttgtcca tgaggatggc
      481 gtttcgctca atacagccgg ggaaatgatg cgcgatgtct ggacgtcact accccagcga
      541 tatccgagcg ttgaactgga tgaatacatc atcatgccag atcatctgca ttgcatcctg
      601 acgctccaca gaagtgttga ggatctggaa ccgccgtcat tgactgacgt gatgggcgac
      661 tttcggagca ggacgaccgt ccgatacatt cagggtgtca aagcagggat ttggccctcg
      721 ttttcgcggc ctctctggga acatcatttt catgaccaca tcattcggga tgagcgggat
      781 atggagtcgc ggcgcatcta cattctccga aatccgtccc gatggctgga aaaacgaaga
      841 gcctgagcct atggccccaa tttgagcgac gatgcgtgga tttcagcaat cgcatagcac
      901 gcaggttgcc gggaagctcg cctcacccgg cttggggtaa cggccacaag ggccgtgggg
      961 acgggttcct tcggtcgctt gcgatacacg agtcgccgcc aggtcaccaa gtgccagtca
     1021 cggcgtgtcg catcgacgag ccgaatgatc cgctggtacc gtagcgtgta cagatcgctg
     1081 gcggcagtgg agtcgggaga gtggtgatgg cgaagcgggt gacgatcgac gtcgaaatcg
     1141 tggtggcgga ttgggacgat ccggacggca tcgccttgcg cgccgcccag cgcgcggata
     1201 tcgacgaccg ctacgggaac agcacgcatg aacccggcgg cgcacccagc ggcgcggaga
     1261 tcagcgtctt cctgatcgcc cgcgatggag acggcatcgc ctgcggatgc ggtgcgctcc
     1321 ggcaactcga cgccgggtcc gccgagatca agcgcatgta cgtcgccccg gaacgccgtg
     1381 gtgccggtgt ctccaccgcc atcctcaacg ccctcgagca acacgcgcgc gctcgtcact
     1441 ggacgacgtt gcggctcgag accggctccc tccttcccga agcaatgcgc ttctacgaac
     1501 gcgagggatt ccggcgcatc ccgaactacg gcatctacat cggcgccgaa gattccatct
     1561 gctacgagaa acacctcgtt tgagccatgg gggaacgatg cggtcgcgtc gtcaccaacc
     1621 ctcgtcgcga tcggaaatcg tggagttcag actccgcgca cgagcctctc gatagcggcc
     1681 gtggatcgcc gctgtcttcg cgacgatcgg ccagccggca ggcatccaac cagcgaactg
     1741 ccgggtgaag catccccttg cttgtcatcc ggatccttgc gcataggcat cgtttgtctt
     1801 taaggcgaat aaaccgttgc ctgttgacgg ctaggcaggg ttgatcgaag gcattgtccc
     1861 gccagaccag tcactgacac ttccgggata ttcaatatga cgagaaatat tgcaaaatca
     1921 ctgacatctt gagaagattg cgcctacggt gcgttgaggc tggccgacac cgtagtccga
     1981 cacaatgtac ggaacgaaca acggagggac catgtcagac ctttgggaca atatcaagga
     2041 cgggattccg gggaacgtag taggcgcggt catcctcctg atcattgggt ggatcgtcgc
     2101 gaggatcgtg gccacagttg tcacgaaagc cgcccgacgg ctcaacctcg acagtcgcct
     2161 cgcgggcctg atgggtcgct cctcagacac aacgcaatcc gattcggcag ggatcctggg
     2221 caagatagcc tattacggca tcctgctctt cgtgctcatc gcggtgttcg acctgctcga
     2281 catgccgctg gtgacacagc cgctcaacga tctgctgtca ggcatattcg cctatattcc
     2341 ccgcctgatt ggcgccgtca tccttatcct gctcgcctgg gttctcgcca cgattctcaa
     2401 gaccgtggtc ttcgagttcc tgcgcggcgt gaatatcgat gcgcgcctga agcgactgca
     2461 gggccaggag ggcgcgatcg gcgaacccgt cgtcgcgcgc gggctctccg atgtcgtgtt
     2521 cgcgctggtg ttcctgctct tcctgccggc aatcctgtcg gccctcaacc tcactggaat
     2581 cctcgaacca gtgcaggaac tctatgacaa gttctttgcg ttcatcccga acctcatcgc
     2641 ggcagcggcg atcatcatca ttggctggtt cgtcgcccgc ttcgtggccc gcctgatcgc
     2701 gagcctgctc gccgcggccg gttcggatcg cttcgccgag cgtgtcggca tctcgtcggc
     2761 ccttggtgcc cggaacctct ccggtacgat cgggctgctc atctacgtgc tgatcctggt
     2821 cccgctgatc attgcgggcc tcaatgcgtt gcagctggat tcgatcacga cgccagccag
     2881 caacatgctg aacagcatcc tgcagttcat cccgcgggcc ttcggcgccg ccgcgatcct
     2941 cgtcatctcc ttcgtggtgg gacggatcgt tgccggcctc atcagcagcc tcctggcggg
     3001 aaccgcctat gactcgctgc tttcgcggct cggtctcggc acgttgaccg atggcacgga
     3061 ggaagcgggc gccgggatct ccctcgcccc gctcaccggc caggcgatcc ttgccgtcat
     3121 catgtggttc gcggcgatcg ccgcactcga gacgctgcag ctcttcaccg tctccgagct
     3181 cctgaccagc ctcctggctc ttgctggcca tatcctgctc ggtgtcatca tcttcgtcgt
     3241 gggtctgctc atcgcccgtt ttgcggggcg gctgatccag ggcgcggtga cggaacaatc
     3301 cggcctgatc cggttcggcg cgcaggcggc gatcatggtg ctcttcggcg ccatggcact
     3361 tcgccagatg ggtgttgcca atgacatcat caatctcgcg ttcggcatcc tgctcggtgc
     3421 ggtcggcatc gcgatcgccg tcgcgttcgg tgtcggcggg cgggaaatcg ccggccggca
     3481 gctcacgcga tgggtggaat cgacccagcg tgaccggaat ccggagaccg aatcctgatc
     3541 ggatagcgat ggatgcgagg tagccagact cgcgcatctc atcgacgcaa ggacagaggg
     3601 aggatcgccg cggcgatcct ccctctgtcc ttgcgtcgat gagatgcgcg agtctggct
//
LOCUS       DASTMD010000415         1632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_127494, whole genome shotgun sequence.
ACCESSION   DASTMD010000415 DASTMD010000000
VERSION     DASTMD010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127494"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFQ54_05985"
     CDS             <1..576
                     /locus_tag="VFQ54_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164577.1"
                     /translation="VIGLGLTYPVMAGQEWLNWRNPSRDWMGINGLEYVDSTTQSYNE
                     PGEYDAINWLWDNNKQDDVMLSAAGCAWQTQIGRPAGATGIPSILGWTDHEEQWHLSD
                     SNIVAETTQRVTDVTTMFNAPPSTDLLDKYGVTLIFIGPVETTGNTGQTSSAGCAPGP
                     FQGASNPDYPGAGWTEVYNAEGVRIYRRDGS"
     gene            696..905
                     /locus_tag="VFQ54_05990"
     CDS             696..905
                     /locus_tag="VFQ54_05990"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEU0164578.1"
                     /translation="MDRLKNLASDFDPGKIEKSLDGVTWPIGKDDLVNVLKQNNVPDS
                     IVSKVESTDASQFENKGDVLKKVGL"
     gene            920..>1632
                     /locus_tag="VFQ54_05995"
     CDS             920..>1632
                     /locus_tag="VFQ54_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU0164579.1"
                     /translation="MGRVLVGTSSWSGHHPFYPPGTRSADQLPFYATRFPVVEVNTTY
                     YGVPIQTTVQGWADRTPDDFIFDVKPPRELTSTPESPGGDPPEPDADLASTFLDALQP
                     LVTARKLGALTFQFPPSYRNTEEHREYLQILPELFPDIPIAVEFRRRDWLDADHADAT
                     LALLENAGLSYTLVDEPQRGTGSVPPVFGVTAPSLALIRFHGRNAAHWYSFSGSSKGR
                     FDWYYQPEELQEWIPRIRRA"
BASE COUNT          341 a          502 c          502 g          287 t
ORIGIN      
        1 gtgatcgggc tcggcctgac gtaccccgtg atggccggcc aggagtggct gaactggcga
       61 aatccgagtc gcgactggat gggaatcaat ggcctcgagt acgtcgacag taccactcag
      121 tcatacaacg agccgggaga gtacgacgcg atcaactggc tctgggacaa caacaagcaa
      181 gacgatgtga tgctatcggc tgctggttgc gcctggcaga cgcagattgg acgccccgcc
      241 ggcgcgacgg gtatcccgtc gatcctcggc tggaccgacc atgaggaaca atggcatctc
      301 tccgattcca acattgtcgc cgagaccaca cagcgagtta cggacgtcac cacgatgttc
      361 aatgcaccgc cgtcgactga tcttctcgac aagtacggcg tgacgttgat tttcatcggc
      421 ccggtggaaa cgaccggcaa caccggacag acctccagcg caggttgtgc gccgggcccg
      481 ttccagggtg cgagtaatcc cgactatccg ggtgcgggct ggaccgaggt ctacaacgcc
      541 gaaggcgtcc gcatctaccg ccgcgacgga tcgtaagtcg ggatcaggcg atcctgtcct
      601 tcgaaccaga ccgaagactc atggcaccgt tgatgcgcct tcggagaacg tgccggccag
      661 cgacggccgg aagtttcagc gaaaggatgc atgtcatgga tcgtctgaag aatctcgcga
      721 gcgacttcga tcccggcaag atcgagaagt cactcgatgg tgtgacctgg ccaattggca
      781 aggacgatct ggtcaatgtt ctgaagcaga acaacgtccc ggacagcatc gtcagcaagg
      841 tcgagagcac ggacgcgagc cagttcgaga acaagggcga tgtcctgaaa aaggtgggcc
      901 tgtagaacgg catatgggta tgggaagggt gctggtcggc acatcgtcct ggagcggaca
      961 ccatccgttc tatccgccgg gcacgcggtc ggccgatcag ctccctttct atgcgactcg
     1021 ttttcccgtc gtcgaggtca atacgaccta ctacggggta ccgatacaga cgacggtaca
     1081 gggctgggcg gatcgcacgc cggacgattt catcttcgat gtgaagccgc cccgtgaact
     1141 cacgagcacc ccggaatccc cgggcgggga cccgcccgag cctgacgccg atctggcgag
     1201 cacattcctc gacgccctgc agccactcgt caccgctcgc aagcttggcg cgctgacgtt
     1261 tcagttcccg ccgtcatacc gaaatacgga agagcatcgc gaatacctgc agatactgcc
     1321 cgaactcttc ccggacatac cgatcgcggt ggagttccgc cggcgggact ggctcgatgc
     1381 cgaccacgca gatgccacgc tggcgctcct cgaaaatgcg gggctcagct acaccttggt
     1441 cgacgagcca cagaggggaa cgggaagcgt gccgccggtc ttcggtgtga ccgcgccgtc
     1501 cttggcgctg attcgcttcc acggccgcaa cgccgcccac tggtactcct tctcgggttc
     1561 gagcaagggg cggttcgact ggtactacca gccggaagaa cttcaggaat ggattccgcg
     1621 catccggcgc gc
//
LOCUS       DASTMD010000416         1582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_127596, whole genome shotgun sequence.
ACCESSION   DASTMD010000416 DASTMD010000000
VERSION     DASTMD010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127596"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VFQ54_06000"
     CDS             complement(<1..816)
                     /locus_tag="VFQ54_06000"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU0164580.1"
                     /translation="MNLGVIWDRDAGVSVVERPEPVAGRGEVVLKVAASGLCGTDLHI
                     AAGEYPLAKSPVVLGHEYAGEIVEIGPGVDGWTRGDRVVVDPNIPCGACRYCHDAKSH
                     LCTDPQTLGVTIDGGLSQYSVVPVSRIYRIPDGLSMAAAALTEPLACALHAVDRANLR
                     AGDTAVVLGAGPIGLLTAALLKTAGASQILVSDPQDVRRARVEEFGGIGITPDDVPVG
                     DADVVMECVGRSQTMLAATQAVRAGGTVVWVGVAAPDATVPVNPYDIFRRELTI"
     gene            complement(820..1392)
                     /locus_tag="VFQ54_06005"
     CDS             complement(820..1392)
                     /locus_tag="VFQ54_06005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEU0164581.1"
                     /translation="MSLSLRDNTPLVVIDVQQGFADPGWGPRNNPGAESRIGQLVAAW
                     REAGRPIFHIQHLSTEPNSPLRPGQPGVEFQDVARPLPDEPVLTKHVNSAFIGTDLEE
                     RLRWNGADAVVIVGLTTNHCVETTTRMAGNLGFNPVLVSDATATFDRVGPDGRLWPAE
                     QVHAMTLANLHDEFARIATTGDVIAAFERE"
     gene            complement(1393..>1582)
                     /locus_tag="VFQ54_06010"
     CDS             complement(1393..>1582)
                     /locus_tag="VFQ54_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU0164582.1"
                     /translation="KLSEEHGVCEVPDGEEGFQVCDRVEIIPNHVCPTVNLMDEMFIA
                     RDGKIVDTWTIAARGKVR"
BASE COUNT          268 a          535 c          521 g          258 t
ORIGIN      
        1 gatcgtcagt tcacggcgga agatgtcgta gggattgact ggcaccgttg cgtccggcgc
       61 ggcaactccg acccagacga ccgttccgcc agcgcgcact gcctgggtgg cggcgagcat
      121 cgtctgcgag cgcccgacgc actccatcac gacatcggca tcgcccaccg gaacatcgtc
      181 cggcgtgatc ccgatgccgc cgaactcctc gacacgagcg cgccgaacat cctgcgggtc
      241 gctgacgagg atctgcgatg ccccggcggt cttcaggagg gcggcggtga gcagtccgat
      301 cggtcccgcg ccgagcacga ccgccgtgtc gccggcgcgg agattcgcgc ggtcgacggc
      361 gtggagggcg caggcgagag gttcggtcag cgccgccgca gccatcgaga gaccgtccgg
      421 gatgcggtag atgcgggaga ccggtaccac cgagtactgg ctgagaccgc catcgatcgt
      481 gacgccgagt gtctggggat cagtgcagag gtgtgacttc gcatcgtggc agtagcgaca
      541 ggcgccacag ggaatgttcg gatcgacgac gacgcggtct ccccgcgtcc agccgtcgac
      601 accaggaccg atctcgacga tctcacccgc gtactcgtga ccgagcacga ccggagattt
      661 ggccaaggga tattctcctg ccgcgatatg cagatccgtc ccgcagagtc ccgaggccgc
      721 gaccttgagt acgacctcgc cgcgacccgc gacgggctcc ggtcgctcca cgacgctgac
      781 gccagcatcc cgatcccaga tcacgccaag attcacgcgt cactccctct caaacgcggc
      841 gatcacgtcg ccggtggttg caatccgggc gaactcgtcg tggagattcg ccagcgtcat
      901 cgcgtgaacc tgctcggcgg gccagagccg cccgtccggg ccgacgcggt cgaacgtcgc
      961 cgtcgcatcg gagacgagca ccggattgaa accgaggttt cccgccatcc gcgtcgtggt
     1021 ctcgacgcaa tggttcgtcg tcagcccgac gatgaccacc gcgtccgcgc cgttccagcg
     1081 gaggcgctcc tcgagatcgg tgccgatgaa ggcgctgttg acgtgtttcg tcagcaccgg
     1141 ttcgtctggc aggggacgtg cgacgtcctg gaactcgaca cccggctgac cgggccggag
     1201 cggggagttc ggctccgtcg agagatgctg gatgtggaag atcggtcgcc ccgcctcgcg
     1261 ccaggcagcc accaactggc cgatccgaga ctcggctccc gggttgttgc gcggtcccca
     1321 cccgggatcg gcaaaaccct gctgaacgtc gatcacgacg agtggcgtgt tgtcccgcag
     1381 tgagagcgac atctagcgca ccttgccgcg ggcggcgatc gtccaggtat cgacgatctt
     1441 gccatcgcgg gcgatgaaca tctcgtccat caggttcacg gtcgggcaga cgtggttcgg
     1501 gatgatctcg acgcgatcgc agacctggaa tccttcctcg ccgtccggaa cctcgcagac
     1561 gccgtgctcc tcgctcagct tg
//
LOCUS       DASTMD010000417         1980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1647895, whole genome shotgun sequence.
ACCESSION   DASTMD010000417 DASTMD010000000
VERSION     DASTMD010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647895"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VFQ54_06015"
     CDS             <1..700
                     /locus_tag="VFQ54_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU0164583.1"
                     /translation="GYAELALANVSSLDVIPDSLAADIAVAAIGTGRTAQMILDAAIL
                     TPKDVAIVTAAASGVGSLLVQSAHHLGATVIALAGGPEKVGLVRKLGTDIAIDYRVEG
                     WHEGLRRYIASGNLQPTISFDGVGGDLGRAAFDLLGPLGRHIVFGWSSGSTTKISVMD
                     LYDRSRTVTAAIGSGAMSRYPDTRPLETRALDAAIAGTAVPIVGQRFALADARDAHRA
                     VSGRGTIGKTVLVP"
     gene            730..>1980
                     /locus_tag="VFQ54_06020"
     CDS             730..>1980
                     /locus_tag="VFQ54_06020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164584.1"
                     /translation="MEPSPLRMKARPTMSSQPLSVEASSELPGPPSIFELWTGRYRGT
                     TLAVFALMFLTAFEALAVTTAMPTVATDLGGIALYGIAFAAPMATGVIAMTISGFWND
                     RSGPRKPILAGVTLFASGLIVAGFAPSMLAIVIGRGIQGLGSGLLSVSLYVMIGQAYE
                     EEMRPRMFTILTGAWILPALIGPAISGLVAEHLGWRWLFLGVPFLVAAAILMLLPTLR
                     RLPAPEGMTKPLGRKPIWAAGAAIAIVVLNWAGQRDIVVWPILLVASLVALGIAIPKL
                     LPAGVWQARRGLPTVITIRGLIGAAFAGTEVYLPLLFKEERGLSPSLAGLVLTSGALT
                     WFAGSWIAGRPEHRLTTPIRIRIGALLIFSGIVTATLTISEQVPVAVGAIGWGLSGLG
                     MGLVYPILSTITLSMSKPEEQGVNS"
BASE COUNT          283 a          685 c          651 g          361 t
ORIGIN      
        1 cggctacgcc gaattggcgc tcgcgaacgt gtccagcctg gacgtgatcc cggattcgct
       61 cgccgccgat atcgcggtcg ccgcgatcgg gaccggccgc acggcgcaga tgatcctcga
      121 cgccgccatc ctgacgccga aggacgtcgc gatcgtcacc gccgcggcct caggcgtcgg
      181 atcgctgctc gtccagagcg cgcatcacct cggcgccacc gtgattgcgc tggccggtgg
      241 tccggagaag gtcgggctgg tgcggaagct cggtacggac atcgcgatcg actatcgggt
      301 cgagggctgg cacgagggac tgcggcgcta catcgcgagc gggaatctcc agccgaccat
      361 ctccttcgat ggcgtcggtg gggatctcgg tcgtgccgcc ttcgatctgc tggggccgct
      421 cggtcgtcac atcgtcttcg gctggtcgtc gggatcgacg acgaaaatct cggtgatgga
      481 tctctacgac cgcagccgca ccgtgacggc ggcaatcggt tccggcgcga tgagtcgcta
      541 cccggacacg cgtccgctcg agacccgggc gctcgatgcc gcaatcgccg gcaccgcggt
      601 cccgatcgtc ggacaacggt tcgcgctggc cgatgcccgg gacgcccatc gcgccgtatc
      661 cgggcgcggc acgatcggca agaccgtgct ggtcccatag aatgacggtc gaatccacaa
      721 cgaatcctga tggagccttc accgcttcgc atgaaagcac gccccacgat gtccagccag
      781 ccgctctccg ttgaagcctc gtcagagttg ccggggccac cgtcgatctt cgaactctgg
      841 accgggcgat atcgcggcac gacgctcgcc gtcttcgcgt tgatgttcct gaccgcgttc
      901 gaggcactcg ccgtgacgac cgcgatgccg accgtcgcca ccgatctcgg cggcatcgcg
      961 ctgtatggca tcgccttcgc cgcgccgatg gcgaccggcg tgatcgcgat gacgatctcc
     1021 ggcttctgga acgatcgcag cggtccccgc aagccgatcc tggcgggggt cacgctcttc
     1081 gcgagcgggc tgatcgtcgc cggcttcgca ccgtcgatgc tcgcgatcgt gatcggccgc
     1141 ggcatccagg ggctcggctc cggcctgctc tccgtctcac tctacgtgat gatcggccag
     1201 gcatacgaag aggagatgcg tccccggatg ttcacgatcc tgaccggcgc ctggatcctt
     1261 ccggctttga tcggtcccgc gatctccgga ctcgtcgccg aacatctggg ctggcggtgg
     1321 ctcttcctcg gtgtcccctt tctcgttgcg gcggcgatcc tgatgctcct gccgacgctg
     1381 cgacgacttc ccgcgcccga aggaatgacg aagccgcttg gccgcaagcc gatctgggcg
     1441 gccggcgcgg cgatcgcgat cgtcgtcctc aactgggcgg ggcaacgcga catcgtggtc
     1501 tggccgatcc tgcttgtcgc gtcgctggtc gcgctcggaa tcgcgatccc gaagctgctt
     1561 ccggccgggg tctggcaagc ccggcgcggg ctcccgaccg tgatcacgat ccgcggactg
     1621 attggagccg cgttcgccgg gacggaggtc tatcttcctc tcctcttcaa ggaggagcgc
     1681 ggcctctcgc cctcgctggc tggactcgtc ctgacatccg gggcgctgac ctggttcgcc
     1741 ggatcgtgga tcgccggtcg accagagcat cggctcacga caccgatccg gatccggatc
     1801 ggcgccctgc tgatcttctc cggcatcgtc accgcgacgc tgacgatcag cgagcaggtc
     1861 ccggtcgcgg tcggggcgat cggctggggt ctttccgggc tcgggatggg gctcgtctac
     1921 ccgatccttt ccacgatcac gctctcgatg tcgaagccgg aggaacaggg cgtcaacagc
//
LOCUS       DASTMD010000418         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2540162, whole genome shotgun sequence.
ACCESSION   DASTMD010000418 DASTMD010000000
VERSION     DASTMD010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540162"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="VFQ54_06025"
     CDS             complement(<1..1895)
                     /locus_tag="VFQ54_06025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU0164585.1"
                     /translation="MAKDEQAPATMLYPRDPSLDPQLVWKGKDEQDRQDLAVPVVPIY
                     IQEKIQPQALIDDLPRVAANPAVAQMDFFGDFDGVDENWESKVEFYQHQQAWSNRLIL
                     GDSLLAMTSLAEKEGLRGKVQTIYIDPPYGIKFGSNWQVSTRKRDVKDGNVADATRQP
                     EQVKAFRDTWQLGIHSYLSYLRDRLVAARDLLTESGSVFVQIGDENVHLVRNLMDEVY
                     GSENFISQITFAKTTGATTALLAGTADYLIWYAKSKEQVKYRALLREKDLTEDRNFRY
                     LETIDGVRRPMTGKEMADQMALPLGSRTFRGNPLTSQSGSTTTRFTYQYEGEAFSPKS
                     GGWKTNLKGMTRLYHARRLMGLGHTLTYVRFADDYNYQVLNDIWMDTKQSGFGDSKTY
                     VVQTMDRVIERCVLMTTDPGDLVLDPTCGSGTTAYVAEQWGRRWITIDTSRVALALAR
                     TRLMAAKFPYYLLTDTPEGIKKETDLTGQVPPPYPTNSDIRKGFVYKRVPHVTLKSIA
                     NNPDIHESMSREQIDAAIAKHAEFETLYDQPYEDSKRVRVTGPFTVESLSPHRVLAPG
                     DENMDGVLSPEEEARQGDFATMILENLRKAGVQNGFKGERLTFERLDPFPGQWINATG
                     EYTESDGA"
     gene            complement(2069..>2705)
                     /locus_tag="VFQ54_06030"
     CDS             complement(2069..>2705)
                     /locus_tag="VFQ54_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164586.1"
                     /translation="LQDNTHIGWLTIPGYLADAADRVYAAIVQGDSGRPTLKPILRPY
                     DTVGSTRYVSFDTTKPVMLTDPAKCHISHIVADTDTWEQKMADVLESMDEVIRYVKNE
                     GLGFTIPYSLDGQARSYIPDFIACIDDGHGPGDLLNLIVEVSGEAKRAKAIKVETAET
                     FWVPAVNTHGGFGRWSFVQIVNPWNAASDIRGHLAKLDATSTPNSAAGQIV"
BASE COUNT          469 a          743 c          896 g          597 t
ORIGIN      
        1 gctccatcgc tctcggtgta ttcaccggtg gcgttgatcc actggccggg aaagggatcg
       61 aggcgttcaa aggtgaggcg ttcgcccttg aacccgttct ggacgccggc cttacgcagg
      121 ttctcgagga tcatggtggc gaagtcacct tgccgcgcct cctcctcggg ggagagcacg
      181 ccgtccatgt tctcgtcgcc gggcgcgagc acgcggtggg gcgagagcga ttcgacggtg
      241 aacggccctg tcacgcggac gcgcttcgag tcctcgtacg gctggtcgta gagcgtctcg
      301 aactcggcat gcttcgcgat ggcggcgtcg atctgctcgc ggctcatgct ctcgtggatg
      361 tcggggttgt tggcgatcga cttgagggtg acgtgaggaa cgcgcttgta gacaaagccc
      421 ttgcggatgt cactgttggt gggatagggc ggcggcacct ggcccgtgag gtcggtctcc
      481 ttcttgatgc cttccggggt gtcggtaagg agatagtatg ggaacttcgc cgccatcaat
      541 cgggtgcggg cgagcgcgag ggcgacgcgc gaggtgtcga tcgtgatcca acgccgcccc
      601 cactgctcgg cgacgtaggc ggtggtgccg gaaccgcagg tcggatcgag cacgagatca
      661 ccgggatcag tcgtcattag gacgcagcgt tcgatgactc tatccatcgt ttgaacaaca
      721 tacgtctttg aatctccgaa tccactttgc ttggtatcca tccaaatgtc attgaggacc
      781 tgatagttgt aatcgtctgc gaatcgaaca tatgtaaggg tatggcccaa tcccatcagc
      841 cgacgtgcgt gatataggcg tgtcattccc ttaaggtttg tcttccatcc gccgctcttt
      901 ggagaaaaag cttctccttc atattggtat gtaaatcgcg ttgtggtgct tccagactgt
      961 gaggttaggg gatttcctct gaacgtacga ctacctagag gaagtgccat ttggtcagcc
     1021 atctccttcc ccgtcatcgg cctacgcact ccatcaatcg tctccaggta tcgaaaattg
     1081 cggtcttcag ttaagtcctt ctctcgcagt aacgcgcggt atttcacctg ttctttagat
     1141 tttgcatacc aaatcaggta atcagcggtt ccggccagta acgctgtagt ggcacctgtg
     1201 gtcttcgcga aagtgatctg gctaatgaag ttctcggaac catacacctc gtccatgagg
     1261 ttgcgcacga ggtggacgtt ctcgtcaccg atctggacga agacactgcc ggactcggtg
     1321 aggaggtcgc gggcggcgac gaggcggtcg cgcaggtacg agaggtagga gtggatgcca
     1381 agctgccagg tatcgcggaa ggccttcacc tgctccggct ggcgcgtggc atctgccaca
     1441 ttgccatcct tcacgtcgcg cttgcgggtg ctgacctgcc agttcgagcc gaacttgatg
     1501 ccgtagggcg ggtcgatgta gatcgtctgg accttgccac gcaagccctc cttctcggca
     1561 aggctggtca tcgcgagcag ggaatcgcca aggatgaggc ggttcgacca cgcctgttgg
     1621 tgctggtaga actcgacctt actctcccag ttctcgtcga cgccgtcgaa gtcgccgaag
     1681 aagtccatct gggcgacggc ggggttggcg gcgacgcgcg gcaggtcatc gatcaacgcc
     1741 tgcggttgaa tcttctcctg gatgtaaatc ggcaccacgg gcacggcgag gtcctgccgg
     1801 tcctgctcgt ccttgccctt ccacacaagc tggggatcga gcgaggggtc gcgcgggtag
     1861 agcatggtgg cgggcgcctg ctcatccttc gccacgaaat cgcgcagttc ctcggtgggg
     1921 atattgacgc gcgtctcgcc gcgatggcgg atggagtcga cggggtttgt ggtcgcggtg
     1981 gctgcggcgg tggttgcggt ggcccggggt gtggtggatc gtggcatggg gttggtggct
     2041 cctcggagat cgacctgcgg tcgataggtc agacgatctg acctgctgcg gaattcgggg
     2101 ttgacgtggc gtcgagtttc gccaggtggc cgcggatgtc gctggctgcg ttccagggat
     2161 tgacgatctg gacgaatgac cagcgaccga acccgccgtg ggtgttgacg gcgggaaccc
     2221 agaacgtctc cgcggtttcc accttgatcg ccttcgcgcg cttggcctcg ccggagacct
     2281 cgacgatcag gttcagcaga tcgcccggac cgtggccatc gtcgatgcag gcaatgaagt
     2341 cgggaatgta ggagcgggcc tgaccatcga gtgaataggg gatcgtgaag ccgagccctt
     2401 cgttcttcac gtagcggatc acctcgtcca tcgattcgag aacatcggcc atcttctgct
     2461 cccaggtgtc ggtgtcggcg acgatgtggg agatgtggca cttcgctggg tcggtcagca
     2521 ttaccggctt ggtggtgtcg aacgagacgt agcgggtgga accgaccgta tcgtatgggc
     2581 ggagaatcgg tttcagcgtc ggacggccgg aatcgccctg cacgatcgcg gcgtagacgc
     2641 ggtcggcggc atccgcgaga tagccaggaa tggtgagcca gccgatgtga gtgttgtcct
     2701 gcagg
//
LOCUS       DASTMD010000419         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2450544, whole genome shotgun sequence.
ACCESSION   DASTMD010000419 DASTMD010000000
VERSION     DASTMD010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2450544"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFQ54_06035"
     CDS             complement(<1..710)
                     /locus_tag="VFQ54_06035"
                     /inference="COORDINATES: protein motif:HMM:NF017812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HEU0164587.1"
                     /translation="MAWTVTTDHLDRLAIGAGILGTGGGGNPYLGKLIARKFINEGAR
                     IEVVSLDEVPDDAVVVSVGGMGSPTVAVERLRRADESLKAMYALERHIGRTFTHIIPS
                     EIGGSNSTIPMIVAAQSGLPVIDGDGQGRAFPELQMETFLIYGIPPTPGALCDHFGHT
                     TVFDHVDDGKTFERYMRAVTIQMGGAAGYAYPAMTGVEVRETAIPDTLSLAVDLGDAV
                     LSARANHVSPVQAALDITG"
     gene            complement(745..1866)
                     /locus_tag="VFQ54_06040"
     CDS             complement(745..1866)
                     /locus_tag="VFQ54_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HEU0164588.1"
                     /translation="MAWTVTTDHLERLAIGAGILGTGGGGNPYMGKIVVSRLVRAGAN
                     IQVVSLDEVPDDATVVTVGGMGSPTIGVERPGSGEESYRALRALEKHIGRKVTHIVSS
                     EIGGGNSTTPLRVATQAGIPVIDGDGMGRAFPELQMKTYIINGIPPTPGALCDYNGHT
                     VVFDHVEDGKTFERYMRAVTIQMGGGAGYSFPLMTGEQVRRTAVPGTLSLAIRLGDAV
                     LNARANHTSPVEAALGVTGGFQLFRGKITDVERRLVGGFARGLLKMEGTGDDLGRTLD
                     IDFQNENLIARRDNGEVIAVVPDLICLVDADTGAPLTTEVVRYGMRAAVLGIPAPVEL
                     KTDAALRSVGPAAFGYPEVEYVPLPGYYANVERVAAVSL"
     gene            complement(1967..2110)
                     /locus_tag="VFQ54_06045"
     CDS             complement(1967..2110)
                     /locus_tag="VFQ54_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164589.1"
                     /translation="MELTKTRAFDARVFVSSLLIESEFGDPESGGEHNAPAWDFARIN
                     RDP"
     gene            complement(2132..>2447)
                     /locus_tag="VFQ54_06050"
     CDS             complement(2132..>2447)
                     /locus_tag="VFQ54_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164590.1"
                     /translation="DFVEQCRQIAPLVSHIHLTDNFGKVQLDAHADQSENLYAGLGDV
                     HVLPGWGSIPFDELEKIPFPRQPIVNLELRPQFFEHLQLASDVTRSFAARIAQNAVVA
                     AD"
BASE COUNT          387 a          753 c          870 g          437 t
ORIGIN      
        1 ccggtgatgt cgagcgccgc ctgcacggga ctcacgtggt tcgcgcgcgc cgacaagacc
       61 gcgtcaccga gatcgacggc gagcgagagc gtgtcgggga ttgccgtctc gcggacctcg
      121 acgccggtca tcgcgggata ggcatagccc gccgcgccgc ccatctggat cgtgacggcc
      181 cgcatgtagc gttcgaaggt cttgccgtcg tcgacgtgat cgaagaccgt cgtgtgaccg
      241 aagtggtcgc agagtgcgcc tggcgtcggc gggattccgt agatgaggaa ggtctccatc
      301 tggagttccg ggaaggcgcg tccctgtccg tcgccatcga tcaccgggag acctgactgc
      361 gcggcgacga tcatcgggat tgtcgagttg ctgcctccga tctcgctcgg aatgatgtgc
      421 gtgaacgtcc tgccgatgtg gcgctcgagc gcatacatcg ccttcagcga ttcgtcggca
      481 cggcgcagac gctcaacggc gaccgtcggc gagcccatcc cgccgacgga gacgacgacg
      541 gcgtcatccg gaacctcgtc gaggctgacg acctcgatcc tggcgccttc attgatgaat
      601 tttcgggcga tcagcttccc gaggtaggga ttgccgccgc cgccagtgcc gagaataccg
      661 gcgccgatcg ccagccgatc gagatgatcc gtcgttaccg tccacgccat gatgctgtgt
      721 gtcctgtttc tcgtgaggtg attgctagag cgagaccgcg gcgacgcgtt cgacattcgc
      781 atagtagccg ggaagcggaa cgtactcgac ctcgggatag ccgaaggcgg cgggtccgac
      841 cgatcgcagc gcggcgtctg tcttcagctc gaccggtgcc gggattccga gcacggcggc
      901 gcgcatcccg tagcggacga cctccgtcgt gaggggagcg ccggtgtcgg catcgacgag
      961 gcagatcagg tcggggacga cggcgatcac ttcgccgttg tcgcggcgag cgatcaggtt
     1021 ctcgttctgg aagtcgatgt cgagcgtgcg tcccaggtcg tcgccggttc cttccatctt
     1081 gaggaggccg cgggcgaagc cgccgacgag tcgacgctcc acgtcggtga tcttgcccct
     1141 gaagagctgg aagccgccgg tgaccccgag cgccgcctcg accgggctgg tgtgattcgc
     1201 ccgggcattg agcacggcgt cgccgagacg aatcgcgagc gagagcgttc ccgggacggc
     1261 cgtccgtcgt acctgctcgc cggtcatcag cgggaacgag tagccggcgc cgccacccat
     1321 ctgaatcgtg acggcgcgca tgtagcgctc gaaggtcttg ccgtcctcga cgtgatcgaa
     1381 gaccacggtg tgcccgttgt agtcgcagag ggcgcccggt gtcggcggaa tcccgttgat
     1441 gatgtaggtc ttcatctgga gttcggggaa ggcgcggccc atcccgtcgc cgtcgatgac
     1501 ggggatgcca gcctgggtgg cgacgcggag cggcgtggtc gagttgccgc cgccgatctc
     1561 gctggagacg atgtgcgtga ccttgcgtcc gatgtgcttc tccagcgccc ggagcgcccg
     1621 gtacgactcc tcgccgctgc cggggcgctc gacaccgatc gtcggcgagc ccatgccgcc
     1681 gacggtgacg acggtcgcat cgtcggggac ttcgtcgagg gagacgacct ggatgttggc
     1741 gcctgcccgc acgaggcggc tgacgacgat cttgcccatg taggggttgc cgccgccgcc
     1801 ggtgccgagg atgccggcgc cgatggcgag acgttcgaga tgatccgtgg tgacggtcca
     1861 ggccattcct gcgttcctgt ctgtagtgat ctgatgagcg gcccggagtc agggggcgtc
     1921 gtgacgatgc cggtcgcatc ggccgggccc attgtgccgg agattctcaa ggatcgcgat
     1981 tgatcctcgc gaagtcccat gccggggcat tgtgctcccc gcccgactcc ggatcgccga
     2041 attcggattc gatgagaagc gaactgacga agacccgcgc gtcgaacgcg cgggtcttcg
     2101 tcagttccaa gcgagcttga gttcgtgaag gctagtccgc ggcgacgacc gcgttctggg
     2161 cgatgcgcgc ggcgaacgag cgggtcacat cggaggcgag ctggagatgc tcgaagaatt
     2221 gcgggcgcag ctcgaggttg acgatcggct ggcgcgggaa ggggatcttc tcgagctcgt
     2281 cgaacgggat cgatccccag cctggaagga cgtggacgtc gccgagtccg gcatagaggt
     2341 tctccgactg gtcggcatgc gcgtcgagct ggaccttccc gaagttgtcg gtgaggtgga
     2401 tgtgggagac gagcggcgcg atctggcggc actgctccac gaagtcc
//
LOCUS       DASTMD010000420         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3165457, whole genome shotgun sequence.
ACCESSION   DASTMD010000420 DASTMD010000000
VERSION     DASTMD010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3165457"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /locus_tag="VFQ54_06055"
     CDS             complement(<1..1807)
                     /locus_tag="VFQ54_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor A family protein"
                     /protein_id="HEU0164591.1"
                     /translation="MKRGSLGAFTLVILLIAVIAGSPAISAQIAQATPVILTPTPIQV
                     IPTTAPTIAPVETVAPTETAVPAETVAPIETAIPAETVVPSETAVPVETTVPSVVPTI
                     DSTVGATDEPTESTVTPEATDVVTETAPVSTLPALPPTFGAATAAVTPDATDEATDVA
                     PVQTVAALPPTFGAATAAVTPDATDDGGWGTPDAAGTPIPVETPVAAGTPVADGTPIA
                     AGTPVVPTQPAATPRMTLRSAAIVNDAEQFAAADAAAPVDFGQASDFNVFVLGDYNAT
                     TSDVEGCLAVGGNVTFTGGFSVGGQAQGCQDALVTGGNLTFPAGTISNGNVVYGGTLT
                     GNPTVPNGTISNGTTTYFDEAEEYFDALSDNLASQPANGTFTSQYGSTTFTGTSTSIN
                     VFNVTAADLNGIHGMTINAPAGSTVIINVSGDNVTFTGYQQWLNGVDASHVIFNFPDA
                     TTITTTGFGFEGSLLAPNATYNFSNGQHNGQIVVRNLTGNGESHNHPFTGNFPPPPST
                     PTPVPTSTPTKTPVPTSTPTKTPVPTSTPTNTPVPTSTPTNTPVPTSTPTNTPVPTST
                     PTNTPVPTSTPTNTPVPTSTPTNTPVPTSTPTNTPV"
BASE COUNT          350 a          635 c          763 g          437 t
ORIGIN      
        1 gcaccggtgt attggtcggc gtgctcgtcg gaaccggtgt attggtcggc gtactcgttg
       61 gaaccggtgt gttcgtcggt gtactcgtcg gcaccggtgt attggtcggc gtcgatgtcg
      121 gcactggtgt gttggtcggc gtcgatgtcg gcaccggcgt gttcgttggg gtcgatgtcg
      181 gcaccggtgt gttcgttggg gtcgatgtcg gcacgggtgt cttcgtcggg gtactcgtcg
      241 gaacgggcgt cttcgtcggc gtgctcgtcg gcactggggt cggcgtggac ggcggcggcg
      301 ggaagttccc ggtgaaggga tggttgtgcg actccccgtt cccggtcagg ttcctgacca
      361 cgatctgacc gttgtgctgg ccattgctga agttataggt cgcgttcgga gcgagaagac
      421 tcccctcgaa cccgaatccc gtcgtcgtga tcgtcgtcgc gtccgggaag ttgaagatga
      481 cgtgggaggc atcgacaccg ttcaaccact gctggtagcc cgtgaaggta acgttgtcgc
      541 ccgacacgtt gatgatcacc gtcgaccccg ccggtgcgtt gatcgtcatg ccgtggatgc
      601 cgttgaggtc cgcggcggtc acgttgaaga cgttgatcga cgtgctggtc ccggtgaatg
      661 tcgtggaacc gtattgactc gtgaacgtgc cgtttgccgg ctggctggcg aggttgtccg
      721 acagcgcgtc gaaatactcc tcggcctcat cgaaataggt tgtcgtcccg ttcgagatcg
      781 tgccgttggg gaccgtcgga ttgccggtca gcgtgccgcc gtagacgacg tttccgttcg
      841 agatcgtgcc ggccggaaac gtgaggttcc caccggtgac gagtgcatcc tggcatccct
      901 gggcctggcc gccgacgctg aagccaccgg taaacgtcac gttcccaccg acggcaaggc
      961 agccttccac gtccgacgtg gttgcgttgt agtcaccgag cacgaagaca ttgaagtcgc
     1021 tcgcctgacc gaagtcgacc ggcgccgcgg catcggccgc cgcgaactgc tcggcatcgt
     1081 tcacgatcgc ggcggagcga agcgtcatgc gcggcgtggc agccggctgc gtggggacca
     1141 ccggcgtacc ggcggcgatc ggcgtgccat ctgcaaccgg agtgccggct gcgacgggtg
     1201 tctcgaccgg gatcggcgtg ccggcagcgt cgggagttcc ccaaccgcca tcatccgttg
     1261 catcgggcgt gacggccgcc gtcgcagcgc cgaatgtcgg cggcagggcg gcgacggtct
     1321 gcactggcgc aacgtccgtt gcctcgtcgg tcgcgtcagg cgtgacggct gccgtagcgg
     1381 ccccgaatgt cggtggcagt gcgggaagcg tcgacaccgg agcggtctct gtcaccacat
     1441 cggtcgcctc gggtgtgacc gtggattccg tcggctcatc ggtcgcccca acggtggagt
     1501 cgatcgtcgg cacgacagac ggtaccgtcg tctcgaccgg gaccgctgtc tccgagggaa
     1561 cgacggtctc cgcaggaatg gctgtctcga tgggagcgac cgtctccgcc ggaacggctg
     1621 tctcggtggg agcgaccgtc tccaccggcg caatcgtcgg cgcagtggtc gggatgacct
     1681 ggattggggt gggcgtcagg atgacgggcg tggcctgtgc gatctgcgcc gaaatcgcgg
     1741 gagacccggc tatcacggca atcaacagga tgacgagggt gaatgctcca agtgaaccgc
     1801 gcttcatcga gactccttga cctggcgagt aatcctcctt gcggagcgca ggcaacatgt
     1861 atggacttgg aaaacgaccc ggcgcgatag ccgggaggaa ctgaccaggc gggtgggtag
     1921 gacttctggg ggagaggtcc tacccgactc atatcctaca gagtactagg cctattgtca
     1981 atacaaaaag gttgtctcta tgccccgagg tctagtccag ggcctagtac ccatacgagt
     2041 atacccatga ccaggtccgg aattccccgg gaggaagcgg cgtttcatct ctccgcgagg
     2101 attccggacg tggaaaacgc cggctcgcga tgagccggcg tgtttgaccg ataacgatta
     2161 tgttgcgccc tctggatcgg aactg
//
LOCUS       DASTMD010000421         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2540546, whole genome shotgun sequence.
ACCESSION   DASTMD010000421 DASTMD010000000
VERSION     DASTMD010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540546"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFQ54_06060"
     CDS             <1..228
                     /locus_tag="VFQ54_06060"
                     /inference="COORDINATES: protein motif:HMM:NF021883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164592.1"
                     /translation="VESGMDRQDAYKLVQRNAHAALAAGEKFQDRVRALPEFTERLTS
                     GQITALFDPWEQLQQVDAIFDRLGLAERVPA"
     gene            225..1121
                     /locus_tag="VFQ54_06065"
     CDS             225..1121
                     /locus_tag="VFQ54_06065"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU0164593.1"
                     /translation="MTTATNGDPTLREYPLFRRGKVRDVYDLGDELLLVATDRLSAFD
                     VVLPTPIVGKGRLLTQLSLWWFGQMGGVVRTQLGNQSLADLDLTDDERSWLDGRTTRA
                     RKAERIDVECVVRGYLAGSGWKEYRSSGTLASESLPEGLLLGSALPAPVFTPAIKNDD
                     GHDENISRPRLHDEIGDDLATTLETTSLEIFARATAIAAAAGFVLADSKFEFGFIDGE
                     LTLIDELLTPDSSRYWDAATLSPGKEPPSYDKQIVRDWLETTDWNKEAPGPAIPEEIV
                     AKAGGRYQAVYDRLVQGNGDHR"
     gene            1118..1405
                     /gene="purS"
                     /locus_tag="VFQ54_06070"
     CDS             1118..1405
                     /gene="purS"
                     /locus_tag="VFQ54_06070"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU0164594.1"
                     /translation="MTNASAALTTWVVEAEVMPKQGVNDPQGDAVMSGLRSLGFAGTK
                     RVRVGKLIRVELDATDESDALEQGRKMCEQLLANPVIEEFTLALTAKEQAS"
     gene            1402..2112
                     /gene="purQ"
                     /locus_tag="VFQ54_06075"
     CDS             1402..2112
                     /gene="purQ"
                     /locus_tag="VFQ54_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU0164595.1"
                     /translation="MNPIVGVITFPGSNGDHDALYSLEHDLGYEVRAIDYRETDLGSL
                     DAVVLPGGFAYGDALRCGAIARFAPVMGSVREFAEAGNPVLGICNGFQVLCEAHLLPG
                     ALLRNASLQFHCFTVNFKVEQADTAWTRGVTPGEILTLPVAHGEGSYFIEPDRLAELE
                     RNRQIVARYVDANGNRTAEANPNGSVASIAAICNAGRNVVGLMPHPERATNALVGGDH
                     GLRILRSLVVASELAAAS"
BASE COUNT          396 a          702 c          732 g          381 t
ORIGIN      
        1 gtcgagagcg gcatggatcg tcaggacgcg tacaagctgg tgcagcggaa tgcgcacgcc
       61 gcgctcgcgg ccggcgagaa gttccaggat cgcgtccggg cgctgccgga attcaccgaa
      121 cggctgactt ccgggcagat caccgcgctc ttcgatccct gggagcagtt gcagcaggtc
      181 gacgcgatct tcgatcgcct cggcctggcg gagcgagtgc cggcatgacg accgcgacca
      241 atggcgatcc gaccctgcgg gagtatccgc tctttcgtcg cggcaaggtg cgtgacgtct
      301 acgatcttgg cgacgagctc ctgctcgtgg cgacggatcg gctctcggcc tttgacgtcg
      361 tcctgccgac gccgatcgtg ggcaagggca gactccttac ccagctctca ctctggtggt
      421 tcggccagat ggggggcgtc gtgcggacgc agctcggcaa ccagtcgctc gccgatctcg
      481 acctgacgga tgatgaacgc tcctggctcg atggccgcac cacgcgcgcc aggaaagcgg
      541 agcggatcga cgtcgagtgt gtcgttcggg ggtacctcgc cggatccggc tggaaggaat
      601 accggtcgag tggcacgctg gcctcggagt cgctgccgga ggggcttctc ctcggttcgg
      661 cactgccggc gcccgtcttc acaccggcaa tcaagaatga cgacgggcac gacgagaaca
      721 tctcccgccc gcggcttcat gacgagatcg gcgacgacct cgcgaccacc ctggaaacga
      781 caagcctcga gatcttcgcc cgggcgacgg caatcgcagc ggccgccggc tttgtgctcg
      841 ccgattcgaa gttcgagttt ggcttcatcg acggtgaact cacgctgatc gatgaactcc
      901 tgacaccgga cagctcccgg tactgggatg cggcgacgct ctcaccgggc aaggagcctc
      961 ccagctacga caagcagatc gtgcgggact ggctggaaac aacggattgg aacaaggagg
     1021 ctcccggtcc ggcgatcccg gaagagatcg ttgcgaaggc aggcggacgc tatcaagccg
     1081 tctacgatcg actggtacaa ggaaatgggg atcatcggtg acgaatgcga gtgcggcgct
     1141 gacgacgtgg gttgtcgaag ccgaagtcat gccgaagcag ggcgtcaacg acccgcaggg
     1201 tgatgcggtg atgtcggggc tccgctcgct cggattcgcg ggcacgaagc gggtgcgggt
     1261 cggcaagctg atccgcgtcg agctcgatgc gacggacgaa tccgatgccc tcgagcaggg
     1321 acggaagatg tgcgagcaat tgctggcgaa cccggtcatc gaggaattca ccctcgccct
     1381 gacggcaaag gagcaggcat catgaatccg atcgttggtg tcatcacatt tcccggcagc
     1441 aatggcgatc atgacgcgct ctattcgctc gaacacgatc ttggctacga ggtccgggcg
     1501 atcgactatc gcgagaccga tctcggatcg ctggacgcgg tcgtcctccc gggcggcttc
     1561 gcctacggcg acgccctgcg ttgcggcgcg atcgcgcgtt tcgcgccggt gatgggttcc
     1621 gtccgcgagt tcgccgaggc ggggaatccg gtcctcggca tctgcaacgg ctttcaggtc
     1681 ctgtgcgagg cacacttgct tccgggcgcc ctgcttcgca acgcctccct gcagttccac
     1741 tgcttcacgg tgaacttcaa ggtcgagcag gccgacaccg cctggacccg gggcgtcacg
     1801 ccgggtgaga tcctcacgct gcctgtcgcc cacggcgaag gctcctattt catcgagcca
     1861 gaccggctcg ccgaactcga gcggaaccgg cagatcgttg cccgctatgt cgatgcgaac
     1921 ggcaatcgca ccgccgaggc gaatccgaac ggatcggtcg ccagcattgc cgcgatctgc
     1981 aacgcgggtc gcaacgtcgt cggtctgatg ccgcatccgg aacgcgcgac gaatgccctt
     2041 gtcggtggcg atcacggcct ccggatcctg cgcagccttg tcgtcgccag cgagttggcg
     2101 gcagcctcct gatcgcacgc gcggcgaccg atcggccgaa tgaagggaat acccagttca
     2161 tgtccaacga gacctacgac aggctgctgt cgcgcattca gcaggtcgac g
//
LOCUS       DASTMD010000422         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1558003, whole genome shotgun sequence.
ACCESSION   DASTMD010000422 DASTMD010000000
VERSION     DASTMD010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558003"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1926)
                     /locus_tag="VFQ54_06080"
     CDS             complement(250..1926)
                     /locus_tag="VFQ54_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0164596.1"
                     /translation="MESHSDDQSKPTVSRLHLNRRRFLAAAATATAIATAGARVPALA
                     QDSATPAAAALPVVPPELTQYAKDWPTPQGNAANQRNATGSTITSKNVGDLEIAWSFP
                     IKATSGSGGLTCTPLIAGQVVYIQDMQSNVFALDRDTGKQIWEAPLNAGTEGPNGVAL
                     GYGMIFASTGDNRELVALDAATGKEKWRTLLSGNTRDGIDMAPAVYNGMVFISTVPGN
                     GKAFYDGGARGILFALDAASGQILWQFYTTDADLWGDPSVNSGGGSWYPPAIDPDGNL
                     YWDIANPAPFEPTSIAGTPVPNGSTREGSNLYSDSMVKLAPNGKLEWFYQANPHDIFD
                     HDLQQSPVFVHVDNNGQPYDVLLGSGKLGKVIAVESTTGNMIWTTKVGEHNQWDDSQW
                     VPPGQTVTVLPGVSGGVESPIAVADGVVYVPVLNLGTGFDSNGFDASTFSYNDATGEL
                     VALDIFDGSIKWDVKLPAGNVSGATVSNDVVFAGALDGIVRAYATADGTLLASYQLDA
                     GLNSPFAVAGDLLIVPAAGAKLVSQTYVAESTPTASTGTGAAVVAFKVKS"
BASE COUNT          427 a          811 c          764 g          452 t
ORIGIN      
        1 ggtccctcga ctgcggcctg gccacgcgct gcgcttgtga tccgggactc cactcgggat
       61 gacggggaaa ggtgtggaca ttcgtgttgt gggacgtgcg acgttcgtcc ggcgccgggt
      121 gggacaggga cgcgccgaag cttctggcaa gcgcaacgca acaccgtggg ctcccttgcg
      181 ggagcccacg gtgttcatcg tcaggaggcg ttcacgagcg gacgcgagcc gcagccgcag
      241 cggcagccgc tacgacttga ccttgaacgc gaccacggcg gcgcccgtgc ccgtactggc
      301 cgtcggcgtc gactccgcga cgtaggtctg gctgaccagc ttcgccccgg cagccggcac
      361 gatcaggaga tcgccagcca ccgcgaacgg agaattgagt ccggcgtcaa gctgataact
      421 cgcgagaagc gttccgtccg ccgtcgcgta ggcccggacg attccatcca gtgccccggc
      481 gaagacgaca tcgttggaga ccgtcgcgcc gctgacattg cccgcgggga gcttcacgtc
      541 ccacttgatc gagccgtcga agatgtcgag cgcgaccagc tcacccgtcg catcgttgta
      601 gctgaaggtc gacgcatcga acccgttcga atcgaatccc gtccccaggt tgaggaccgg
      661 cacgtagacg actccgtcgg caacggcgat cggcgactcg acgccgccgc tcacgcccgg
      721 caggacggtg accgtctgtc ccggcggaac ccactggctg tcgtcccact ggttgtgctc
      781 gccaaccttc gtcgtccaga tcatgtttcc ggtcgtggac tcgacggcga tcaccttgcc
      841 gagcttgcca ctcccgagca ggacgtcata cggctggccg ttgttatcga cgtgcacgaa
      901 cacggggctc tgctggaggt cgtggtcgaa gatgtcgtgg ggattcgcct ggtagaacca
      961 ctcgagcttg ccattcggcg ccagcttcac catggagtcc gagtagagat tggacccttc
     1021 acgggtggac ccgttcggta ccggcgtccc cgcgatgctg gtcggctcga agggcgcggg
     1081 attcgcgatg tcccagtaga ggttgccatc aggatcgatg gccggcgggt accaggagcc
     1141 accaccgctg ttcacggacg ggtcacccca gagatccgcg tcggtggtgt agaactgcca
     1201 gaggatctgg ccgctggcgg catcgagggc aaacaggatg ccgcgggctc cgccgtcgta
     1261 gaaggccttg ccgttcccgg gaaccgtgct gatgaagacc atgccgttgt agacggccgg
     1321 ggccatgtcg atgccgtccc gcgtattgcc ggagagcagc gtccgccact tctccttgcc
     1381 cgtggcggca tcgagcgcga ccagctcgcg attgtcaccc gtcgaggcga agatcatgcc
     1441 gtagccaagg gcgacaccgt tcggcccctc ggttccggca ttgagcggag cctcccagat
     1501 ctgctttccg gtatcacgat cgagcgcgaa gacattgctc tgcatgtcct ggatgtagac
     1561 cacctgtccc gcgatcagcg gcgtgcaggt caggccaccc gaaccacttg tggccttgat
     1621 cgggaagctc caggcgattt cgaggtcgcc aacgttcttc gaggtgatcg tcgatccggt
     1681 cgcattccgc tgattggcgg cgtttccctg gggagtcggc caatccttcg cgtattgcgt
     1741 cagctcaggc ggcacgaccg gcaacgcggc cgcggcaggc gtcgcgctgt cctgggcaag
     1801 ggcagggaca cgcgcccccg ccgtggcgat cgccgtcgcc gtcgcggctg ccgccaggaa
     1861 acggcgacga ttcaggtgca acctcgacac agtcggcttc gactggtcgt ccgaatgaga
     1921 ctccacggag cactctccct tcgacgtccc gcactggaca ctttcaaaac gacaacgtgt
     1981 tgctgtactg acaccctatc gccctgtgaa tctgctgtca agagattgct gatggtcgca
     2041 ttactatcgc gttgtcacct cgggcacgag gctcgattcg tgccccgcag ttgcatgatc
     2101 catcccgcat gacgtcgcca tcgacgctcg cggtcacgcc cctgcccccc gtttgccccg
     2161 atcaatcccc tgtcacggaa gcatcctgcc aatcctggtt gaacgacagc cacgttctcc
     2221 ggcgcgtttc acgtcgatgc ccccacatgc gacatgtcgt cacccgggaa tcccctcccg
     2281 ctccggaact gcgcgtcaat ggacttgaca gtccaatttc aatcgggaat acttgagcat
     2341 tatggaccga atggtccaat catgaatgag ggtgccggca ccctcaccaa tcgtcgtggt
     2401 tggctcgatc agccgctcga cgcgagcaag aaagcgaatc gccccatgga cgcc
//
LOCUS       DASTMD010000423         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_39716, whole genome shotgun sequence.
ACCESSION   DASTMD010000423 DASTMD010000000
VERSION     DASTMD010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39716"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFQ54_06085"
     CDS             complement(<1..462)
                     /locus_tag="VFQ54_06085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922182.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU0164597.1"
                     /translation="MAVRATEISDLLKQQIQGFSDQATVTNVGTVIGIGDGVATVYGL
                     TQCMASELVEFTKSGILGLALNLGEDTVAVIVMGDYTSIEEGDEVRTTGKIASVPVGD
                     ALLGRVVDALGQPIDGKGPIKTDKTRDIERIAPGVIKRQDVKTALQTGIKAI"
     gene            complement(595..1125)
                     /locus_tag="VFQ54_06090"
     CDS             complement(595..1125)
                     /locus_tag="VFQ54_06090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU0164598.1"
                     /translation="MASTSARRYAQAIIELAQEEGNFDAWQRDLTELGTLVGDDSAAG
                     FFANPNISDDEKFTVVENVLKDLRPETRNLAKILIDRRKIAIVPDVVALFDEAVLARK
                     GVALVDVTTAETLDDQGQELVRQHLKTMLGKDIQLRLHVDPEIIGGIIARSGDQVIDG
                     SVTNQLRRLRTRLYAA"
     gene            complement(1219..1719)
                     /gene="atpF"
                     /locus_tag="VFQ54_06095"
     CDS             complement(1219..1719)
                     /gene="atpF"
                     /locus_tag="VFQ54_06095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU0164599.1"
                     /translation="MSDLGLNGWQFVVQLVAFLVFVGLLWKYAVGPIVQMLDQRQQRI
                     SESMQAAQRMEEELKTAQARNEETLQQARREAQEILTSARQSSEQIIARAREEANGQA
                     EEYLSRAQDTLRQETERARQQLRQELADLAVSAAGRILRKEIDPSTQTRLIEETLVDA
                     SRVSPN"
     gene            complement(1854..2081)
                     /gene="atpE"
                     /locus_tag="VFQ54_06100"
     CDS             complement(1854..2081)
                     /gene="atpE"
                     /locus_tag="VFQ54_06100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEU0164600.1"
                     /translation="MDTEAARMIGSGIAMGFGAIGPGIGVGLAVKGALEAMGRNPEAT
                     GEIRTTMILGAALAEAVAIYAFVMAIIIKFV"
     gene            complement(2256..>2682)
                     /locus_tag="VFQ54_06105"
     CDS             complement(2256..>2682)
                     /locus_tag="VFQ54_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit a"
                     /protein_id="HEU0164601.1"
                     /translation="AMALVTFTLVQVVAIRSQGVGGRIKHMAQPPWLFPIELVSELSR
                     ILSLTARLFGNVFAGEVLLGVMYSAGSALKIAVIPFLFPVLFLGLEVLFGSIQAIVFS
                     LLTAIYLVLAAPPHDDDADGHQDEDAHAVRPVAASAAGD"
BASE COUNT          482 a          874 c          796 g          530 t
ORIGIN      
        1 gattgccttg atcccggtct ggagcgccgt cttcacgtcc tggcgcttga tgacgcccgg
       61 cgcgatgcgc tcgatgtcgc gggtcttgtc ggtcttgatc gggcccttgc catcgatcgg
      121 ctgcccgagg gcgtcgacca cgcgaccgag cagcgcgtcg cccacgggga cggacgcgat
      181 cttgccggtc gtgcgaactt cgtcgccttc ttcaatggag gtgtagtcac ccatcacgat
      241 gaccgccacc gtgtcttcac ccaggttgag ggcaagtcca aggatgccgg acttggtgaa
      301 ctccacgagc tcactcgcca tgcattgcgt aaggccataa accgtcgcga cgccgtcacc
      361 gattccaatc acggtgccga cgttcgtcac ggttgcctgg tctgagaacc cctggatctg
      421 ctgcttgagc agatcactga tttcggtcgc gcgtaccgcc atgatcgctc cgttcttccg
      481 cggcgtcagt gccgcgacgc gcggtcctga taattgccgt ggccgttgct actgtttcgc
      541 cgacccggtc cgcaccacga gaggaacggc ccggggattc gcgcttcgac gtcgttacgc
      601 cgcgtaaagc cgcgtgcgca ggcgccgaag ctgattcgtc acgctgccat cgatgacctg
      661 atcaccggag cgcgcgatga ttccgccgat aatctccggg tcgacatgca ggcgaagctg
      721 gatatccttg cccagcatcg tcttcaggtg ctgtcgaacc agttcctgtc cctggtcgtc
      781 cagggtttcc gccgtggtca catcgaccag ggcgaccccc ttccgcgcca ggactgcctc
      841 atcgaagagc gccaccacgt ccggaacgat cgcgatcttg cgccggtcga tgaggatctt
      901 cgccagattc cgggtctctg gcctcaggtc cttgaggaca ttctcgacga cggtgaactt
      961 ctcgtcgtcg gagatattgg gattggcgaa gaaaccagcg gcactgtcat caccgacgag
     1021 ggttccgagc tcggtcagat cccgctgcca ggcgtcgaag tttccttctt cctgcgccag
     1081 ctcgatgatg gcctgtgcgt agcgtctcgc cgaagtgctc gccatcgctt gttccccgtc
     1141 tcgctcacgt tgcgtacgca ggaggcaaaa ggcagtttgg ccgagttgcc tcatccctcc
     1201 tgccttctgc ccacctcgtt agttcggaga cacgcgggag gcatccacaa gtgtttcctc
     1261 gatcagacgc gtctgcgtcg agggatcgat ttccttgcgc aggatgcgac cggcggcgct
     1321 taccgcgagg tccgcaagtt cctggcgcaa ctgctgccgc gcgcgttcgg tttcctggcg
     1381 aagcgtatcc tgagcacggc tcagatactc ttccgcctgg ccatttgcct cttcccgcgc
     1441 ccgggcaatg atctgttcgc tactctgtcg cgccgacgtc aggatttcct gagcctcacg
     1501 ccgcgcctgc tgcaacgttt cctcgttgcg ggcctgcgcg gtcttcagct cttcttccat
     1561 ccgctgggcg gcttgcatgc tctcgctgat gcgctgctgg cgttgatcga gcatctgcac
     1621 gatcgggcca accgcgtact tccagagcaa gccaacgaag accaggaatg cgaccagctg
     1681 gacgacaaat tgccatccgt tcagtccaag gtcactcacg ttcggccttc cttcgcacca
     1741 agacgtcgtt ccgggccatc cggcgatcgg agatcacggg atggggaaac ggatcaggac
     1801 gccggcgccg ctcactgcac gatgcctggc gccgaacgcc tgatccggag gaactagacg
     1861 aacttgatga tgatcgccat aacgaaggcg tagatcgcga ccgcttccgc gagtgccgca
     1921 ccgaggatca tcgtcgttcg gatctcaccg gtggcttccg gattgcgacc catcgcctcg
     1981 agggcgccct tgaccgcgag accgacgcca atacctgggc caatcgcacc gaaacccatg
     2041 gcgatccccg aaccaatcat gcgtgccgct tctgtatcca tgccgcttgg gctcctcctg
     2101 cggggtacca gccggacgtg ttcatacacg cgacggcccc attgccatgg tcaagacgac
     2161 tcctgccgcc tcgtcatcct gataagaaca cgaaggtttc gagaaccgaa gcgtcgtcgt
     2221 gcggcgacgc cccggggttt ctcgcgaagg tggggtcagt ctcccgccgc tgacgcagcg
     2281 accggtcgta ccgcatgcgc atcttcatcc tgatgcccgt ccgcgtcatc gtcatgtggt
     2341 ggtgccgcaa ggacgaggta gatcgcggta aggagtgaga agacgatcgc ctggatactt
     2401 ccgaagagca cttccagccc gaggaaaagg accgggaaaa gaaacgggat gactgcaatc
     2461 ttcagtgccg aaccggcgga gtacatgaca cccagcagga cctcaccggc gaaaacattg
     2521 ccgaagagac gggcggtcag cgagaggata cgcgagagct cggagacgag ttcgatcggg
     2581 aacaaccagg gcggctgggc catgtgcttg atgcgcccac caaccccctg gctgcgaatc
     2641 gccaccacct gaacgagcgt aaacgtcacg agcgccatgg cg
//
LOCUS       DASTMD010000424         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3166053, whole genome shotgun sequence.
ACCESSION   DASTMD010000424 DASTMD010000000
VERSION     DASTMD010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3166053"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..345)
                     /locus_tag="VFQ54_06110"
     CDS             complement(136..345)
                     /locus_tag="VFQ54_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEU0164602.1"
                     /translation="MELSARNQIPGTIVAVEIGVVTAEITVDIGNDQTIVSVITKGSA
                     ERLGLAIGDKVVAVLKATDVMIGKL"
     gene            complement(541..1884)
                     /locus_tag="VFQ54_06115"
     CDS             complement(541..1884)
                     /locus_tag="VFQ54_06115"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEU0164603.1"
                     /translation="MSDGKAFPAGFIWGAATAAYQIEGAVREGGRGESIWDRFSHTPG
                     RIANGDTGDIADDHYHRVPDDIQVMRELELGAYRFSIAWPRILPNGRGQVNGEGLDFY
                     DRLVDGLLAAGIEPWVTLYHWDLPQALQEGGGWAHRSTADAFADYTDVVTRRLGDRVK
                     NWITINEPWVISVLGHLLGIHAPGSQSLPEALAVGHHLLLAHGNAVEVVKSNVRDSRV
                     GITLNLSHVYPTSDKEADLDAARIADGTFNRWYLDPIFGREYPEDIVAMFGDAMPEIR
                     AGDFATIARGTDFLGINTYNPIHVNHDPKGLFGAIGTPRVGGKRTAMDWIVEPDGFRD
                     LLVRVQTDYAPARIYVTENGSAWNDRPPVDGRVEDPDRIDYLHGHLLAARDAIAKGVK
                     LDGYFAWSLLDNFEWSEGYAKRFGIVHVNYTTQERTIKDSGRFYTEAIRANGENL"
     gene            complement(1881..2642)
                     /locus_tag="VFQ54_06120"
     CDS             complement(1881..2642)
                     /locus_tag="VFQ54_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0164604.1"
                     /translation="MNKSQIGIQLYTLRTLTATDFDGTLAQVAAAGYPGVEFAGFGDL
                     SAAEMRGLLDQHGLKAFSAHIPLQAFEASIDQVISDLQTIGATYGIVPWIAPEARSFA
                     NFQKLGEQFNEYGTKLQAAGLKFAYHNHNFEFEEKSEAGQTIWDALIQNTEPGVVSFE
                     LDAFWAEVGGYDAAQVIRATPNRIRLVHLKDASKDDAKKDVPFGEGILDWGAILSASK
                     DAGVEFYLTEQDNPNPDDPMSDATIAYRNAEKAAQ"
BASE COUNT          526 a          938 c         1007 g          584 t
ORIGIN      
        1 ggcgatgaca agcaggatcg gacgaagtgg cgcgcggcgg gtagtggcgt tcattcgagt
       61 gtcattcccc tctgtttcgg aatttcgaga atccgatacg acgcaaatgg acctgcggga
      121 cgaatcctgt cagagtcaga gcttgccgat catcacatcg gttgccttga ggacggcgac
      181 gaccttgtcg ccgatcgcca gcccgaggcg ctcggccgag cccttggtga tgacggagac
      241 gatcgtctgg tcgtttccga tgtcgacggt gatctccgcg gtgacgacgc cgatttcgac
      301 ggcgacgatc gtaccgggaa tctgattgcg cgcactcagt tccatatcga ggccctcctg
      361 cgggtagacg acgatcgaat agtcaaaagc cgactgaacg tgcccatcgt accttacggg
      421 cgagagtgag ggaagatgga tcgtgccgtc gccgctgccc catgcagcga ccgccggggc
      481 cggatcactc tgatccggcc ccggctcgtc ttcctgcctg gctcctggat ttcgggattg
      541 ctagagattc tcgccattcg cgcggatggc ttcggtgtag aagcgcccgg agtccttgat
      601 cgtccgttcc tgcgtcgtgt agttgacgtg gacgatgccg aagcgcttcg cgtagccttc
      661 cgaccactcg aaattgtcga gcagcgacca ggcgaagtag ccgtcgagct tgacgccctt
      721 cgcgatcgcg tcgcgcgccg cgaggaggtg accatggaga tagtcgatgc gatcgggatc
      781 ctcgacgcga ccgtcgaccg ggggacggtc gttccaggcg gagccgttct ccgtcacgta
      841 gatgcgggcc ggtgcgtagt cggtctggac gcggacgaga aggtcgcgga agccgtctgg
      901 ctcgacgatc cagtccattg cggtccgctt gccaccaacg cggggcgtgc cgatcgcgcc
      961 gaagaggcct ttcggatcgt ggttgacgtg gatcgggttg taggtgttga tgccgaggaa
     1021 gtcggtcccg cgggcgatcg tcgcgaagtc gccggcgcga atctccggca tcgcgtcccc
     1081 gaacatcgcg acgatgtctt cgggatactc acgaccgaag atcggatcga gataccagcg
     1141 gttgaaggtc ccgtccgcga ttcgcgccgc atcgagatcc gcttccttgt cactcgtcgg
     1201 gtagacgtgg gagaggttga gcgtgatccc gaccctggag tcacggacat tcgacttgac
     1261 gacttcgacc gcgttgccat gggcgagcag gagatggtga ccgacggcga gcgcctccgg
     1321 gaggctctgc gatcccgggg catgtatccc gagaagatgc ccgagcacgg agatcaccca
     1381 gggctcgttg atcgtgatcc agttcttgac gcgatcgcca aggcggcggg taacgacgtc
     1441 ggtgtagtcg gcaaacgcat cggcggtgct gcgatgcgcc cagccgcctc cctcctgcaa
     1501 cgcctggggc agatcccaat ggtagagcgt gacccatggc tcgatgccgg cggcaaggag
     1561 gccgtcgacg agccggtcgt agaaatcgag cccctcgccg ttgacctgac cgcgtccgtt
     1621 gggaaggatc cggggccagg cgatcgagaa gcgataggcg ccgagttcca gctcccgcat
     1681 gacctggatg tcatctggaa cccgatggta atgatcgtcg gcgatgtcgc cggtatcgcc
     1741 gttggcgatc ctgcccggcg tgtggctgaa ccgatcccag atcgattcgc cccgcccgcc
     1801 ttcgcggact gccccttcga tctggtaggc ggccgtcgcc gccccccaga tgaagcccgc
     1861 gggaaacgct ttcccgtcac tcattgcgcg gctttttcgg cgttgcgata ggcgatcgtg
     1921 gcgtcggaca tcggatcgtc cggattcgga ttgtcctgct cggtgagata gaactcgaca
     1981 ccggcatcct tcgatgcgga gaggatcgcg ccccagtcga gaattccttc gccgaacggg
     2041 acgtccttct tcgcatcgtc cttcgaggca tccttgagat gcacgaggcg gatgcgattc
     2101 ggcgtcgcgc ggatgacctg ggcggcgtcg tagccaccga cctcagccca gaaggcgtcg
     2161 agctcgaagg agacgacacc cggctccgtg ttctggatca gcgcatccca gatcgtctgg
     2221 ccggcctcgc tcttctcctc gaactcgaag ttgtggttgt gataggcgaa cttcagtccg
     2281 gccgcctgga gcttcgtgcc gtactcgttg aactgctcgc cgagcttctg gaagttggcg
     2341 aaggagcgag cctccggcgc gatccagggg acgatgccgt acgtcgcgcc gatcgtctgc
     2401 aagtcggaga tgacctggtc gatcgacgcc tcgaacgcct gcagggggat gtgggcgctg
     2461 aaggccttga ggccatgctg gtcgagcaat ccgcgcatct cggcggcgga aagatcgccg
     2521 aagccggcga actcgacgcc ggggtagccc gccgccgcga cctgggcgag cgtgccgtcg
     2581 aaatcggtcg cggtgagggt gcgcagggtg tagagctgga taccgatctg ggacttgttc
     2641 atgcggattc ctttccgtct tgtgcgtcgc tccgtcgcga cgccggatat acaacgtcta
     2701 ctacgttccg tagcgggcca ccccgtgtgg cccatcgacc gaaggtcgat atgatctctt
     2761 ccacagaacg tcgtgcccaa agatattcgc gatgaaccgt agacgcggcc ttcatggccg
     2821 ccaggcccgg caacgccggg cctcgtgcgt tggcgaacga tcttccccgg agaaccacaa
     2881 ttcccgtacg tccggcggat cgccttcggc gacccgtggc gaccacaagg gtcgcgtcta
     2941 cggttgccgg aatgggactg atcctcgtgc accgctcgcc aacagtcata tccggctgcg
     3001 ccggatggac cacacggggt ggcccgctac ggatgttcga aatcttgggc cccac
//
LOCUS       DASTMD010000425         4149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1290596, whole genome shotgun sequence.
ACCESSION   DASTMD010000425 DASTMD010000000
VERSION     DASTMD010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290596"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..884
                     /locus_tag="VFQ54_06125"
     CDS             372..884
                     /locus_tag="VFQ54_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEU0164605.1"
                     /translation="MLEIQVRTVAAQRVVTEKKTVDQAQLMEWLPGAMGRTYEAAEAA
                     GGWTPSNELAWLGRENLKAEPVFIVIYEGNPNEGPTEIEVCTLVAADIPDGDGVRTIP
                     AHQEAFAHLTRTEADPSVLGAAYEELASWILGGGKQMAGAPREIYYTDYFGAAPDDIV
                     CDLAWPVTGS"
     gene            1209..2081
                     /locus_tag="VFQ54_06130"
     CDS             1209..2081
                     /locus_tag="VFQ54_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU0164606.1"
                     /translation="MAKSDFGAHGIWSAQFATGSPAEVSRHVAELDAQGWGAIWFPGM
                     GGGDDVYGIAERLLQAGNQAKVAVGVAGIWELDPAATARTHRRLQDAYGDRLVSGFGV
                     SGPDRARAAGKEFGSPLAAMRKFLDDLDAADPPLPRDERVIGTLGRRITMLAGERAAG
                     VHPFLVTPVAVARDRALIGPDALIAPYLPVVLETDPVRARAIARHGIGMFLGIPSYQG
                     NLLRLGFGEEDFAPGGSDRLIDSLVAWGDLAKITTRIREFQDAGADHLALHALTGSDA
                     FPVAEWKELSGLLS"
     gene            complement(2192..3271)
                     /locus_tag="VFQ54_06135"
     CDS             complement(2192..3271)
                     /locus_tag="VFQ54_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0164607.1"
                     /translation="MTDAKIRLGFVNGDHLHFNGLVQAAFESPTAEVIGAVIDDEELR
                     NYFADKYPQLRMFESSDELYEIGDPNAIVTCADNFNAAAIVAEAAERGVHVMKEKPMA
                     ASLAVAEEMATTAARHDIRLMINWPNNWSPAIQHLKTLTDAGEIGNVWQIHHRAGHGG
                     PPRNYQSGSPVGRVGWDWLIDRERNGGGAAVDFCSYGAVLSRWIMGQPSRVLGLGGRY
                     SKDFFTVEDNAVMILGYPKGHSICEGTWTQPAMPVHIPGMVYGDQGAIAILNQTELKI
                     ANQGRPGEHMNLPREGTVIEAPALPAHYKSPVDYFTYSLLHDEPFTGIVAPEISRDAQ
                     EILEAGLASMESGQEIGLPLPSFLN"
     gene            complement(3393..>4149)
                     /locus_tag="VFQ54_06140"
     CDS             complement(3393..>4149)
                     /locus_tag="VFQ54_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0164608.1"
                     /translation="PIAISLKDADAMVEAAKTAGVKLTMGYQPRFGNLWPRVKQIIDD
                     GYLGDIMGLRLASSAPSSHGVPWFLKKELAGGGVLIDWGIYTAFTLNWWLGPVERVFA
                     STRIFRDEVMVRGELVKGIDVEDTVAAQIDFKSGAIGTWYAAWAVKANHGGTAIDGSE
                     GSLVINGHTLRLKSTKTNDPESISGWHEFDVPDPILVDMHYRKIEHLVDSILDDKPLV
                     LTGEDGRDALELVLGIYKSAETGQPVSFPVPRD"
BASE COUNT          697 a         1339 c         1382 g          731 t
ORIGIN      
        1 actcgcctcg cgacaggccg ttacccgcga cagccgtcat gaaccgacct cgcgacatgg
       61 cgtcacccgc gacaaccgtc gccaacccgt ctcgcgacag gctgtcaccc gggacaaccg
      121 tcatgaaccg gcctcgcaac aggccgtcac ccgcgacaac cgtcaccaac cggcgacacg
      181 atcacctcat tcacctggcc atcgccgcca cgatcctctg ccatgtcgga aggtggtaca
      241 cccatgtctt cggtcggtac ctttatctct tgccgccgtc gcaaggggga agaagcgtgt
      301 gccgtacccg cgcccgtatg cttgcttcca cgttccgaat caccaagacc acctcgtgaa
      361 ggaggattcc gatgctggaa atccaggtgc gcacggtcgc cgcgcagcgc gtcgtgaccg
      421 agaagaagac cgtcgatcag gcccagctca tggagtggtt gcccggcgcg atgggccgca
      481 cctacgaggc ggcggaggca gccggcgggt ggactccgtc gaacgaactc gcgtggctcg
      541 gtcgggagaa cctgaaggcg gagccggtct tcatcgtcat ctacgaaggc aatccgaacg
      601 aggggccgac cgagatcgag gtctgcacac tggtggcggc tgacatcccg gatggtgacg
      661 gcgtgcgcac gatcccggcg catcaggaag cgttcgcgca cctcacccgg acggaggccg
      721 atccgtcggt gctcggcgcg gcctatgagg agctggcgag ctggattctc ggcggcggga
      781 agcagatggc gggcgctcca cgcgagatct actacaccga ttacttcgga gcagcgccgg
      841 acgatatcgt ctgcgatctt gcctggccag tgaccgggag ctagcgcgcc gttccatccc
      901 cccaatcccg attcagcaga cgccgtcgcc ggatcgtgtt cccagcgacg gcgtctgctg
      961 caagtcacct cgatcgagtt gccctcgtgg cccgcggatt ctccgtctcg ccggccctcg
     1021 accgcctgta tcgcccggat caggcggtca tgggcgcgaa tgttgcggga acgtatcgat
     1081 cgacagcggt cattcgggga gggatcagcg ccgcggtgtt ccctcagccg ggcgacgagc
     1141 ctggaaccct cgctcttccc tcctccgatc ctgatcgcca gatgtgacta tggatcagga
     1201 gattcacgat ggcaaaatcc gatttcggag cacatggaat ctggagtgcc cagtttgcga
     1261 ccggttcgcc ggcggaggtg tcgcgacacg tcgcggaact cgacgcccag ggatggggag
     1321 caatctggtt tcccgggatg ggtggcggtg atgatgtgta cggaatcgcc gagcggttgc
     1381 tccaggcagg aaaccaggcg aaggtcgcag tcggtgtggc gggcatctgg gaactcgatc
     1441 ccgccgcgac agcacgcacc caccggcgcc ttcaagacgc gtatggagac cggctggtat
     1501 ccggtttcgg tgtcagtggg cccgatcggg cgcgggcagc cggcaaggag ttcggatcgc
     1561 cgctcgctgc gatgcggaag ttcctcgatg atctcgatgc ggcggatccg ccgctcccca
     1621 gggacgagcg cgtgatcggc acgttggggc ggcgcatcac aatgcttgcc ggcgagcgtg
     1681 ccgcgggagt ccatcccttc ctggtgacac cggtcgcggt ggcgcgggat cgggcgctga
     1741 ttggcccgga cgcactgatc gcgccatatc tcccggtcgt gctcgagacc gatcccgtgc
     1801 gggcgcgggc gatcgcgcgg cacgggatcg ggatgtttct cggcattcca tcctatcagg
     1861 ggaatctcct ccggctcggt ttcggggagg aggatttcgc tcccggcggg agcgatcgcc
     1921 tgatcgactc gctggtggca tggggtgatc ttgccaagat cacgacccgc atccgggaat
     1981 tccaggatgc cggggccgac catcttgccc tccacgcgtt gacgggatcg gatgcgttcc
     2041 cggtagcgga gtggaaggaa ctgagcggtc tgctttcgta gggcggcgat cgaacccgtt
     2101 gccaacggtg gcgatccccg ggcgaacgcg aggcgggacc gccgctgccg gaccgcagtc
     2161 cggacggcct gtcccgcccg cgtggctggc gctagttcag gaacgacggc agcggaagac
     2221 cgatctcctg gcccgattcc atcgaggcga gacccgcctc caggatctcc tgcgcgtccc
     2281 gcgagatctc cggtgcgacg atgccggtga acggctcgtc gtggagcagg ctgtaggtga
     2341 agtaatccac cggggacttg tagtgcgccg gcagcgcggg cgcctcgatc acggtcccct
     2401 cgcgcgggag gttcatgtgc tcgcctgggc gtccctggtt cgcgatcttc agttcggtct
     2461 ggttcaggat cgcgatcgcg ccctggtcgc cgtagaccat ccccgggatg tggaccggca
     2521 tcgccggctg cgtccaggtg ccctcgcaga tcgagtggcc cttcggatag ccgaggatca
     2581 tcactgcgtt gtcctcgacc gtgaagaagt ccttcgagta gcggccacca agaccgagca
     2641 cgcggctcgg ctggcccatg atccagcggc tgagcacggc gccataggag cagaagtcga
     2701 ccgctgcgcc accaccgttg cgctcgcggt cgatcagcca gtcccagccg acgcgaccga
     2761 cagggctgcc ggactggtag ttccgcggcg gtccgccgtg accggcgcgg tggtgaatct
     2821 gccagacgtt gccgatctca ccggcatcgg tgagcgtctt caggtgctgg attgccggtg
     2881 accagttgtt cggccagttg atcatcaggc ggatgtcgtg gcgggcggcc gtcgtcgcca
     2941 tctcctcggc gacggcgagc gacgccgcca tcggcttctc cttcatgacg tgaacgcccc
     3001 gctccgcggc ctcggcgacg atcgccgccg cgttgaagtt gtcggcgcag gtgacgatcg
     3061 cgttcggatc gccgatctcg tagagctcgt cagacgattc gaacatccgg agctgcgggt
     3121 acttgtcggc gaagtagttg cgaagctcct cgtcgtcgat cacggcgccg atcacctcgg
     3181 cggtcgggct ctcgaaagca gcctggacga ggccgttgaa atgaaggtga tcgccgttga
     3241 cgaaaccaag tcggatcttt gcgtcagtca tgagaaacgt ggtcctttca agaaacctgc
     3301 ttcatcccgt cccggcgacc cacgtggtcg cctgccggac gaggcgggga gatgccccgc
     3361 cgggacggag ccgtgaattg cggcccggga gcctagtcgc gcgggaccgg gaagctgacc
     3421 ggctggcccg tctccgcgga cttgtagatg ccgagcacca gctcgagcgc gtcacgacca
     3481 tcctcaccgg tcagcacgag cggcttgtca tcgaggatgg aatcgacgag atgctcgatc
     3541 ttccggtagt gcatgtcgac gaggatcgga tccggcacat cgaactcgtg ccagccgctg
     3601 atcgattccg gatcgttggt cttcgtgctc ttgaggcgaa gcgtgtgacc gttgatgacg
     3661 agtgatccct cgctgccatc gatcgcggtg ccgccgtgat tcgccttcac cgcccaggcg
     3721 gcgtaccagg ttccgatcgc accgctcttg aagtcgatct gcgcggcgac ggtatcctcg
     3781 acgtcgatgc ccttgacgag ttcaccacga accatgacct cgtcgcggaa gatccgggtc
     3841 gacgcgaaga cgcgctcgac cgggccgagc caccagttga gggtgaaggc ggtgtagata
     3901 ccccagtcga tgaggacacc accaccggcg agctccttct tcaggaacca cggcaccccg
     3961 tgggatgagg gggccgacga ggcgagccgg agacccatga tgtcgccgag gtagccgtcg
     4021 tcgatgatct gctttacgcg cggccagagg ttgccgaagc gcggctgata gcccatcgtc
     4081 agcttcacgc ccgccgtctt cgcagcctcg accatcgcat cggcatcctt gagcgagatc
     4141 gcgatcggc
//
LOCUS       DASTMD010000426         3363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2540893, whole genome shotgun sequence.
ACCESSION   DASTMD010000426 DASTMD010000000
VERSION     DASTMD010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540893"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..939)
                     /locus_tag="VFQ54_06145"
     CDS             complement(43..939)
                     /locus_tag="VFQ54_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164609.1"
                     /translation="MSANPSTNQPAVGYGEVFDRGYQHYTGERLGRRQAFRSLIGYSI
                     KRAMGIRKPWTSKILPILLYAAAIIPLIVIIGVAAVVPTFSFGSYSGYFAAIFTVEGI
                     FVATIAPEMLCPDRRERTLPLYFARAISRTDYILAKLIATFILTLTMSLVPAVILWFG
                     RQLVADHPWRAMRDNVDDLWKLILVGTLTALFFGTLGLLISSLTDRKGIAVTVIVVGF
                     LIITAATQAAFNVTEDSWRRYLIFLSLNNLIGGILDHLFHDVNVDMVTVADFPLGVYI
                     AYILVVVALSILIIRWLYRPRD"
     gene            complement(929..1897)
                     /locus_tag="VFQ54_06150"
     CDS             complement(929..1897)
                     /locus_tag="VFQ54_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0164610.1"
                     /translation="MTQTDSGGPPVISTRGIRKTFGKFVALENLDLSIPRASVGLLGA
                     NGAGKTTLIRTLLGLIKPDRGTATVVGFDTQANGVSVRERVGYMPEADALPSSTTAAD
                     FVAHMAEMSGLPTRAARQRAADVLYQVGLEEERYRLIKGFSTGMRQRVKLAQAIVHDP
                     ELVFLDEPTAGMDPNGREEMLELVERIYKQLGIAVVFSSHILEDIERVCYYVVILDGG
                     RLVTAQPLGGLGTIGGEVVVEIDGDRDAFAGLIQQNGVAVRSIESESGARELVIDIQD
                     ERVYDLIRDAAVHQNVALRSLRSRSRSLEDIYLGDVGAMREETHVG"
     gene            2310..3284
                     /locus_tag="VFQ54_06155"
     CDS             2310..3284
                     /locus_tag="VFQ54_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU0164611.1"
                     /translation="METPQEYLNTLSEGLADAVAAAEASVVSVHGRRRLPGTGTVWQA
                     ADKTFVVTASHIVEREENLAISNGDGELVAATLLSRDFGTDLAVLRVDDLKGTPITLR
                     TAPARVGNLVLAIGRPFGVTSATFGSITTIGSIRFRHFQTPTLIHAETTPLPGFSGGP
                     LIDATGAAIGINTSGLMRRGRPLGGATGLVTIPTAQVDAIVSDIVEYGHVRYGWVGVS
                     IQQVELPESAREAFNEQEIGLLITSVAAGSPAANAGIVVGDILVSMGEQALTDVADLQ
                     HLLGGRQIGTPIAVNLLRGGSQVTLEVTPAERPERTEEERERRGPRRS"
BASE COUNT          625 a         1091 c         1072 g          575 t
ORIGIN      
        1 atccgtctgc acctgtgtgt ccacgttcgg gcctcctttc agctagtctc gcgggcgata
       61 gagccagcgg atgatcagga tggagagcgc gaccacaacc aggatgtagg cgatgtagac
      121 acccaacggg aagtccgcca ccgtcaccat gtcgacgttc acgtcgtgga agaggtgatc
      181 gagaatcccg ccaatgaggt tgttgaggct caggaagatg aggtagcggc gccatgaatc
      241 ctcggtgacg ttgaatgccg cctgcgtcgc cgccgtgatg atcaggaagc cgacgacgat
      301 cacggtcacc gcgatgccct tgcgatccgt cagggacgag atcagcagcc cgagcgtgcc
      361 gaagaagagc gccgtcagcg tgccgacgag gatcagtttc cagagatcgt cgacattgtc
      421 ccgcatcgct cgccacgggt ggtcggcaac gagttgacga ccgaaccaga gaatgaccgc
      481 gggcacgagc gacatcgtca gcgtgaggat gaaggtcgcg atcagcttgg cgaggatgta
      541 gtccgtacgg gaaatcgccc gtgcgaaata gagcgggagc gtccgctctc tccggtcggg
      601 gcagagcatc tcgggagcga ttgtcgcgac gaagatgccc tcgaccgtga agatcgccgc
      661 gaagtatccc gagtacgacc cgaaggagaa ggtgggaacc accgccgcga ccccgatgat
      721 cacgatcagg gggatgatcg ccgccgcata gagcaggatc gggaggatct tggatgtcca
      781 cggcttgcgg atgcccatcg cccgcttgat ggagtagcca atgagcgagc ggaacgcctg
      841 tcgccgcccg agacgctcgc cggtatagtg ctgatacccg cgatcgaaga cctctccgta
      901 gccgacagcg ggctggttcg tcgaaggatt agccgacatg ggtctcctcc cgcatggcgc
      961 cgacgtcgcc gaggtagata tcctcgagcg atcgcgagcg ggatcgcagg gagcgcagcg
     1021 ccacgttctg atggacggcg gcgtcccgga tcaggtcgta gacgcgctcg tcctggatat
     1081 cgatcacgag ttcccgggcg ccggattcac tctcgatgga tcgcaccgcg acaccgttct
     1141 gctggatgag cccggcgaag gcatcgcgat cgccatcgat ctcgacgacg acctcgcctc
     1201 cgatcgtccc gagcccgccg agcggctgcg ccgtcaccag gcgaccaccg tcgaggatca
     1261 caacgtagta acagacgcgc tcgatatcct cgaggatgtg ggacgagaag acgacggcga
     1321 ttccgagttg cttgtagatg cgctcgacaa gctccagcat ctcctcgcga ccgttcgggt
     1381 ccatgcccgc ggtcggctca tcgaggaaga cgagctccgg atcgtggacg atcgcctgcg
     1441 ccagcttcac ccgctgccgc atgccggtgg agaagccctt gatcagacga taacgctctt
     1501 cctcgagccc gacctggtag agcacgtcgg cggcgcgttg ccgggcggcg cgcgtcggca
     1561 ggccgctcat ctccgccatg tgcgcgacga aatcagcggc cgtggtcgac gacggcagcg
     1621 catccgcctc gggcatgtat cccacgcgct cgcgaacgga gaccccgttc gcctgggtat
     1681 caaagccgac gacggtcgcc gtaccgcgat cgggcttgat caggcccagc agcgtgcgaa
     1741 tcagcgtcgt cttgccggcg ccattcgcgc cgaggagccc caccgatgcc cgggggatgg
     1801 agagatcgag attttcgagg gcgacgaact tgccgaatgt cttccgaatc ccccgcgtgc
     1861 tgatcaccgg gggtccgcca ctatccgtct gtgtcattcc catgcgccgt ggggacctcc
     1921 tcgtcgtccg cgtcctgaag atgcctgcaa tggggatagg tcgcgacaca cggacagcgt
     1981 tcaaataagg gagctcttgc cccgttccca tgcaacgctt gagagtctac cagcaccatt
     2041 ccccggcggg agcgcggccg ttgacccatc cccacctatc caaaggatta gccgtctcct
     2101 gctcggacaa cggatgccgc ttcccctcgc gtaaccgtac tcttctctgc agatcgttgc
     2161 gacgaccgaa gatctggaat cagacgtccg atcctgcgtg ccgtgaatgc ccgcgtcgct
     2221 ttcctggcac ttcgatcggt tgcaatgagt tcacgcttct ctccatacaa gtgacgcgcc
     2281 cgttcgcgtc gaggaaggga ttacacgaca tggagactcc gcaggagtat ctcaacaccc
     2341 tgagcgaagg tctcgccgac gccgtggctg cggcagaggc gagcgtcgtt tccgttcacg
     2401 gtcgccggcg ccttccgggc accggtaccg tctggcaagc tgcggacaag acctttgtcg
     2461 tcacggccag ccacatcgtc gagcgcgagg agaacctcgc gatctcgaat ggcgacggcg
     2521 aactggttgc cgcgacgcta ctcagtcgcg acttcggcac cgatctcgcc gtcctccgcg
     2581 tcgacgatct gaagggaacg ccgatcacgc tgcgaaccgc gcccgcccgc gtcggcaacc
     2641 tggtgctcgc gatcggccgg ccgttcggcg tgacctcggc gaccttcgga tcgattacga
     2701 cgatcggctc gatccggttc cgtcacttcc agacgccgac gctgatccac gccgagacga
     2761 ccccgctgcc cggcttttcc ggcggtcccc tcatcgatgc gaccggcgcc gcgatcggca
     2821 tcaacacctc cgggctgatg cgccggggcc gaccgctcgg tggcgccacc ggactcgtca
     2881 cgatcccgac ggcgcaggtg gacgccatcg tctccgacat cgtcgagtac gggcacgtcc
     2941 gctacggttg ggtgggtgtc tcgatccagc aggtcgagct gcccgaatcg gcgcgagaag
     3001 ccttcaacga gcaggagatc ggcctgctga tcaccagcgt cgccgcgggg agcccggcgg
     3061 caaacgcggg gattgtcgtg ggtgatattc tggtatccat gggcgagcag gcattgaccg
     3121 atgttgcgga cttgcagcat ctcctcgggg gacgccagat tggcactccg atcgccgtca
     3181 acctcttgcg aggaggatcc caggttaccc ttgaggtgac accggccgag cgcccggagc
     3241 gcaccgagga agagcgcgaa cgccgcggac caaggcgatc gtgagcgata caaccggcgg
     3301 gccgcgcatc ttcatcgcgc ccgcatccac gactgggggt ccgatgatct cacggctcaa
     3361 tgt
//
LOCUS       DASTMD010000427         1901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_40045, whole genome shotgun sequence.
ACCESSION   DASTMD010000427 DASTMD010000000
VERSION     DASTMD010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40045"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1221)
                     /locus_tag="VFQ54_06160"
     CDS             complement(55..1221)
                     /locus_tag="VFQ54_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU0164612.1"
                     /translation="MIVAGGKLSKWNKQYRLFDVPIPFVLEILTVIEFVTLDRPQGSL
                     LLAQGLILPLCLRTRNPRATLLFICIVAMVVALLNMPTVALAAVLIGGYSVASLRNQR
                     DAIWGWVAAEVVVVFCAEQFNPATGGLIASIIFLTGLTLAAFFLGTTVRANRLYLASL
                     VERTRQLEFERDQQARLAVAAERTRIARDMHDIIAHSLSVVVTLAEGASATVKKEPGA
                     ATDAMQQAASTGRQALTEVRRLLNVLRDDEPPSLVPLPELSRLDGLIDDVRATGLSVI
                     MTTSGMPGLLPPSAESTIFRIVQESLTNVLKHANSPTRVLVTLAWQDNGVTIDVRDDG
                     TESMDMRRNVGNEGYGLRGMRERVALFDGELESGPIAPRGWLVRARLSFLPEVA"
     gene            complement(1262..>1901)
                     /locus_tag="VFQ54_06165"
     CDS             complement(1262..>1901)
                     /locus_tag="VFQ54_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0164613.1"
                     /translation="RWDSLSAGNQATFDAIGVSLTGVVLAQLIVSVLGVLIVTSEYST
                     GMIRASLAAVPRRLMLLTGKIVVSGAVALAASLSACFLAFLLGQVILGDHGVTLGSSD
                     AMRAILGAAFYLTSISVIASAIGFIVRSTAGGIAATLGLLLVLPILGQVLPYKWVNDI
                     LDYLPSSAGMAIYSTPSDSTASISATSGVIALIVWMVVSIAGAAFVLVRRDA"
BASE COUNT          432 a          592 c          541 g          336 t
ORIGIN      
        1 gacgaaaccc catcctcacc aacgactgat cgtcaaccag caggacactg attgtcatgc
       61 gacctccggc aagaacgaga gtcgcgcccg taccagccag ccgcgcggcg caattggccc
      121 agattccagc tccccgtcaa agagcgcgac ccgctcgcgc atccctcgca ggccgtatcc
      181 ctcattacct acgtttcgtc gcatgtccat cgactctgtt ccatcatccc tgacgtcgat
      241 cgttacccca ttatcttgcc acgcaagcgt cacgagtacg cgggtcggag aatttgcgtg
      301 cttgaggacg ttcgtcagac tttcctgtac gattcgaaat attgtggatt ccgccgaagg
      361 tggcaataag cccggcatgc ccgatgtggt catgattacc gagagacccg tggcccgaac
      421 gtcatcgatc aacccgtcga gtcgggacag ctccggtagc ggtacaagcg acggaggctc
      481 gtcgtctcgc agaacattca agagtcggcg aacctctgtc aaggcttgtc ttccagtgga
      541 agcagcctgc tgcatggcgt ccgttgcggc accgggttcc ttcttaaccg tcgccgacgc
      601 gccttcagcc agcgtgacaa ccactgacaa gctgtgcgca atgatgtcat gcatatctcg
      661 agcgatccgc gtgcgctcgg cggccacagc caatcgcgcc tgttgatccc gctcgaactc
      721 gagttgcctc gtccgctcaa cgagtgaggc gaggtagagc cggttcgctc gcaccgtcgt
      781 accgagaaag aacgccgcca gcgtgagccc ggtgaggaaa atgatggatg cgatcaggcc
      841 accagtcgct ggattgaact gctccgcgca gaagacgact acgacttccg ctgctaccca
      901 accccagatc gcatcacgct gattgcggag tgacgctacg gaataccctc cgatcaggac
      961 ggcggcgaga gccacagtcg gcatgttgag cagagcaacg accatcgcca caatgcaaat
     1021 gaacagcagc gtcgcacgcg gattgcgagt ccgcagacag agcggcagaa tcagtccttg
     1081 cgcgagaagg agactcccct gcggtctgtc cagggtcacg aattcgatga cggtcaagat
     1141 ttcgagaacg aacgggatcg ggacgtcgaa caaccggtac tgcttgttcc atttgctcaa
     1201 ctttccgccc gcgacgatca tgccatcaac tccacatagt gatcctctat ctgcgtctag
     1261 cttaggcatc ccgccgaacg aggacaaagg cggctcccgc aatggacacc accatccata
     1321 caatgagtgc gatcaccccg ctggtggctg aaatgctggc tgtcgaatcc gaaggggtgg
     1381 agtagatcgc cataccggca ctactgggca gatagtccag gatgtcgttc acccatttat
     1441 aggggagcac ctgacccaat atcgggagca ccagcagcag tccgagggtg gcggcgatgc
     1501 caccggccgt tgaccggacg ataaagccaa ttgccgaggc gatgacggaa atcgacgtca
     1561 ggtaaaacgc agcaccgaga atcgcccgca tggcatccga cgatccgagc gtcaccccgt
     1621 ggtcgccgag gatcacctgc cccagcagaa acgccaggaa gcatgctgac aggctggcag
     1681 ccagggctac tgcccctgat accacgatct tcccggtgag cagcatcagg cgacgaggga
     1741 ccgccgcgag cgaggcgcga atcatgcctg tcgagtattc gctggtcacg atgaggacgc
     1801 cgaggaccga gacgatgagt tgtgccagaa caacgcccgt aagggacacg ccgatggcat
     1861 cgaaggtcgc ttgattcccg gcactgagtg aatcccaacg g
//
LOCUS       DASTMD010000428         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1112453, whole genome shotgun sequence.
ACCESSION   DASTMD010000428 DASTMD010000000
VERSION     DASTMD010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112453"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFQ54_06170"
     CDS             complement(<1..222)
                     /locus_tag="VFQ54_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164614.1"
                     /translation="MFATACLIPLLAVVLFFLDRGIQRNTDQIVNSENTIASLSSQNM
                     STYLNGVVDMLDKLSARPEIVGLNQEDSKN"
     gene            633..>1556
                     /locus_tag="VFQ54_06175"
     CDS             633..>1556
                     /locus_tag="VFQ54_06175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0164615.1"
                     /translation="MTRIVVIGGGIVGASAAYHLAKSGAETILVDRKDVGQATAAGAG
                     IVSPGPTRHDTEAFFALATPAVAYYPELVAGLAEIGQTKTGYDVVGELYLSENDEQAA
                     ELEEIYARVIERRDRGVPGIGELSWLDSQQVKDAFPAVRDVPRAIHISGAARVDGAFI
                     RDAMLAGSEHHGTRRRFGDAELIVEDGKVSGVAVDGERIEADGVVVAGGAWTNRLLAQ
                     LGYALPVAPQRGQILHVTMGETDTSRWPILAWFDDLYMLTFAPNRVVAGATRETGSGF
                     DVRMTPGGIKYVLDIALRIAPGIANGTLHEIR"
BASE COUNT          301 a          447 c          532 g          276 t
ORIGIN      
        1 gttcttggag tcttcctggt tgaggccgac gatctccggc cgggccgaga gcttgtcgag
       61 catgtcgacg acgccgttga ggtaggtcga catattctgg ctggaaagac tggcgattgt
      121 gttttcgctg ttgacgatct gatccgtgtt gcgctggata cctcgatcca ggaagaagag
      181 cacgacggcc agaagcggga tgaggcatgc cgtcgcaaac aacacaaatc gggtgcggag
      241 agaccatcgt ctcatcatgg atgacgtgcc ttgagaggcg cctggaccac ggctcaggga
      301 ggcgctgcga tgaatacgaa aaaaggtgtg gccgatgaga gacacacctg agcaattgtc
      361 ccaatcatcg gtcatcgatt gcgatataca agggagaccg cgttcctatc cgaaagagtc
      421 tcaattgccc gatactggtc accctggcgg ctccgcggcg ccgatgtctc acactattaa
      481 cggttgcgca tgaacgaagt gtaggcgggc tgtgcgtgaa tgcaatgcat cgccggcggc
      541 cagtcggcgt gatgcggtgc aggtagagac gagttgtgcg acgatcggat ctgtctgacg
      601 ggaatacgtt tttggaagcg aggagcagaa gcatgacccg gatcgtcgtc atcggaggcg
      661 gaatcgtcgg cgcaagcgcg gcctatcacc tcgcgaagtc cggcgcggag accatcctgg
      721 tcgatcgcaa ggacgtgggg caggcgacgg cagccggcgc cggtatcgtt tcgccgggtc
      781 cgacccgcca cgataccgag gcattcttcg cgctcgccac acctgccgtc gcctactacc
      841 cggaactcgt cgccgggctg gccgagatcg gacagacgaa gaccgggtat gacgtcgtgg
      901 gtgaactcta tctttccgaa aatgacgagc aggcggcgga actcgaagaa atctacgcgc
      961 gagtcatcga acgccgcgat cgcggcgtcc ccgggatcgg tgagctcagc tggctcgatt
     1021 cacagcaggt gaaggacgcg ttcccggcgg tgcgtgacgt gccacgcgcg atccacattt
     1081 ccggcgcggc gcgagtcgac ggcgccttca tccgtgacgc gatgctggcc ggctccgagc
     1141 atcatggcac ccggcgaagg ttcggcgacg cggaactgat cgtcgaggat ggcaaggtgt
     1201 ccggggttgc ggtcgacggc gagcggatcg aggcggacgg cgtcgtagtc gcgggtggag
     1261 cgtggacgaa ccggctgttg gcgcagctcg ggtacgcgct tccggtcgcc ccgcagcgcg
     1321 gccagatcct tcacgtcacg atgggcgaaa ccgacacgag ccgctggccg atcctcgcct
     1381 ggttcgacga tctctacatg ctgaccttcg caccgaatcg cgtggtggcg ggagcgaccc
     1441 gggagacggg ctccgggttc gacgtccgga tgacgccggg cggtatcaag tacgtgctcg
     1501 acatcgcgct tcggatcgca ccgggaatcg cgaacgggac gcttcacgag atccgc
//
LOCUS       DASTMD010000429         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1022793, whole genome shotgun sequence.
ACCESSION   DASTMD010000429 DASTMD010000000
VERSION     DASTMD010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022793"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFQ54_06180"
     CDS             <1..390
                     /locus_tag="VFQ54_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164616.1"
                     /translation="TLPFDSADPAAPISIGASKLSHARASIVNVNLAFGDAVIAESGT
                     VMTITKGSVVALAIGLAQGAGLTSQLIKMYEGIDKRWPSKSAAKTQSDGEKVGGLWDA
                     IPVLKDFPVDFSLAFQADITDSVNATA"
     gene            427..774
                     /locus_tag="VFQ54_06185"
     CDS             427..774
                     /locus_tag="VFQ54_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164617.1"
                     /translation="MSKNIAEKAETMVRTYTATKDYSKDAKQLAKEGWRVAEVTRHER
                     RRGCMRILMMGVIFAWIYPPKPEIIVRYERAILNKGFARCYSCGTINRIPKFERGQPH
                     VFTCSNCGAELRD"
     gene            793..1986
                     /locus_tag="VFQ54_06190"
     CDS             793..1986
                     /locus_tag="VFQ54_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0164618.1"
                     /translation="MTKPSRTLRYGDGFLTQRGDATVQARWKDGPKMRAKTFRGETLE
                     HAWEQAEDHLRQIARDRRDGRYTSPSDLTIKQLLEEYRDRGATRWSMNTQATYGQLVT
                     THIVPHLGSRRIIDLTPRMVQLWIDGLHKQGLSAAVIENAKIVLSGACREAVQMGIMR
                     MNPTSGLRLPAKPKSRMPTWTSAEVYSVLTAISEIEWRKPYVELWMRTFYTLAITTGM
                     RPGELRALMWKDVDLEDGRLTCHRSMTRDPNFKTIVGEQTKGGRARVIAIPEETVAIL
                     KEYRRAQVTRRLGAIGWFNTGIVLDRGNGNHIPLTTLENRHKQVIAHAGVTSIRLHDL
                     RHTAATLLLEADAHPKIVSDLLGHSSIAITMDRYVHVSTDLQRSATNVLGSIISGTNK
                     GQKPA"
     gene            2303..2737
                     /locus_tag="VFQ54_06195"
     CDS             2303..2737
                     /locus_tag="VFQ54_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164619.1"
                     /translation="MSAEAPDLDGYRLASQLREVWRVVLVHVRISFEERKISAPYLGT
                     QTTVLAIVQRAEAPSERDWLQVGLSPRRPYSIQDLTQILKDLPDPFEVELPDDIFDLE
                     MRAMGTHLVKRVIREELKRRTTETVEDDLPASEERESKSANR"
BASE COUNT          658 a          871 c          850 g          545 t
ORIGIN      
        1 acgcttccgt ttgactctgc tgatccagca gcgccaataa gcatcggcgc gtcgaagctt
       61 tcacatgcac gcgcatccat cgtcaacgtg aatttggcat ttggagacgc ggtcattgcc
      121 gaatcgggga cagttatgac gatcacgaag ggatcggttg tcgctctcgc gatcgggcta
      181 gcccaggggg ccggattgac ttcgcagctc atcaagatgt acgagggcat cgacaagcga
      241 tggccatcaa agtcggccgc caagactcag tcggatggcg agaaggttgg cggtctttgg
      301 gatgcgatcc ccgtccttaa ggatttcccc gtcgactttt cgctcgcgtt ccaggcggat
      361 atcactgata gcgttaatgc tacagcgtag gctaccggtc gaccataccg gaattgaagg
      421 atgactatga gcaagaacat cgccgaaaaa gccgagacga tggtgcggac gtatacggcg
      481 accaaggact atagcaagga tgcaaaacaa ctggccaagg aaggctggcg ggtcgccgaa
      541 gtaacaaggc acgagcgacg tcgtgggtgc atgcgcattc tcatgatggg tgtgatcttc
      601 gcctggatct atccgcccaa gccagaaatc atcgttcgct acgagcgcgc catactcaac
      661 aagggcttcg cccgatgcta ctcctgcggc acgatcaata gaatccccaa gttcgagcgg
      721 ggacaaccgc atgtcttcac ctgctcgaac tgtggcgccg aactgcgcga ctagacgagc
      781 ggacggattg ctatgacgaa gccgagtcgc acactccgtt acggagatgg attcctcacg
      841 cagcgcggtg atgccaccgt ccaggcacgg tggaaggacg gcccgaagat gcgggcaaag
      901 accttccgtg gcgagacgct ggagcacgcc tgggagcaag ccgaggacca tctgcggcag
      961 atcgcccgcg atcggcgaga cggtcgctac acgtcaccgt ccgacctcac gatcaaacag
     1021 ctgctcgagg aataccgtga ccgcggcgcc acacggtgga gcatgaatac gcaggcgacg
     1081 tacgggcaac tggtcacgac acacatcgtg ccgcatctcg gctcgcgccg gatcatcgat
     1141 ctcacgccca gaatggtcca gctctggatc gatgggctgc acaagcaggg cctcagcgcc
     1201 gcggtcatcg agaacgcgaa gatcgtcctc tctggcgcct gccgggaagc ggtgcagatg
     1261 ggcatcatgc ggatgaaccc gacgtcggga ctccgtctgc cggcgaagcc caagagccgg
     1321 atgccgacgt ggacctccgc cgaggtctac agcgtgctca ccgctatctc ggagatcgaa
     1381 tggcgaaagc cgtatgttga actctggatg cgcacgttct acaccttggc gatcacgacc
     1441 ggcatgcggc cgggcgagct ccgcgcgctg atgtggaagg atgtcgatct cgaggacgga
     1501 cggttgacct gccatcgctc gatgacgcgt gacccgaact tcaagacgat cgtcggcgaa
     1561 cagaccaaag gcggtcgggc acgcgtcatc gcgattcccg aagagacggt cgccatcctg
     1621 aaggagtatc gccgcgctca ggttacgcga cgactcggag ccatcggctg gttcaacacg
     1681 ggcatcgtgc tcgatcgcgg caacgggaat cacatcccgc taacgacgct ggagaatagg
     1741 cacaagcagg tcatcgcgca cgccggtgtg acctcgatcc ggctgcacga tctccggcac
     1801 accgccgcga cgctgctcct ggaggcggac gcacatccga agatcgtctc cgatctcctt
     1861 ggtcactcaa gcatcgccat cacgatggac cgctacgtcc acgtctccac cgatctccag
     1921 cggagcgcga cgaacgtcct ggggagcatc atcagcggca caaacaaggg ccaaaaaccc
     1981 gcgtgaggtc ttgcgtgaac taaccgcgtg aagtcgtgta aaaccgtgtg aagtcgtggc
     2041 aagaaacgcc agtaaatacg cgtattttcc aaacccggcc cttggcaccc gaaattgcgg
     2101 attcccttat ccatctaggg ggtccgcctt tttgtgtccg acgttgcgtg aggtcctgcg
     2161 tgaacttgcc ttgatgatac gtataagcca taggcaggaa tccagatgtt gtcgtgcttc
     2221 cttccatata catcaccgag caacagcctt cgacaatttg gaacatctag attctaagta
     2281 gaacgtatga tctaatactt gtgtgagtgc agaagcgccc gatttggatg gataccgtct
     2341 cgcgagtcaa ttgcgcgagg tttggagggt tgtgctcgtg catgttcgta tcagctttga
     2401 ggagcgcaag atcagtgcgc cctatctcgg gacgcagacc acggtgcttg ccatcgttca
     2461 gcgagcggaa gcgccatcag agagagactg gctccaggtg ggactgtcgc ctcggcgccc
     2521 gtactccata caggacctta cccagatcct caaggatctc cctgatccat tcgaagtaga
     2581 actcccagac gacatctttg atctcgagat gcgggccatg ggaacacatc tcgtcaagcg
     2641 cgtaattcgc gaggaactca aacggagaac cacggagacg gtcgaggacg acctgccagc
     2701 atcagaggaa cgggaatcca agagtgccaa ccggtgaatt cgcactttca cgagcacccc
     2761 atccgccctc ttgacgtccc tctattcaga acatatgatc tactcgctac ggcgacacca
     2821 tgacgtgttc ctatccgccg tagcgtcccc tcatcccact gggcctcggg ccgtcacccg
     2881 gggctcagga ttgagcgcag aagcgccacc cctatccgct gcga
//
LOCUS       DASTMD010000430         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2808978, whole genome shotgun sequence.
ACCESSION   DASTMD010000430 DASTMD010000000
VERSION     DASTMD010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2808978"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VFQ54_06200"
     CDS             <1..790
                     /locus_tag="VFQ54_06200"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0164620.1"
                     /translation="DLPEVIDLVATAPDDKEIQGWLIPPVGFDPTSDARHPVILEIHG
                     GPHAMYGYALFHEMQLMASKGYAVVFSNPRGSNGYGEEFTTCTRGVWGEADSPDILAV
                     LEKALELPWLDPTRLGITGGSYGGYMTNWIIGHDQRFKAAVTQRCVSNFYSFFGTSDI
                     GFDFGAFEFDGLPWKDAAKLLKHSPISYVDQIETPLLILHSEQDLRCPIEQSEEMFTA
                     LRFLEKEVAFVRIPGESHGLSRNGTPSRRLARLHHLIGWFDSHL"
     gene            812..>2562
                     /locus_tag="VFQ54_06205"
     CDS             812..>2562
                     /locus_tag="VFQ54_06205"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0164621.1"
                     /translation="MNETLSLRELADRLATQLDRAYSPAHGHAARGGEPLVESFEAFA
                     GDARAAGLPLEDVMTAGIDTLQTFFRLFGNSPASDPATMRAAGVALASAARTHILPIR
                     TFEDTANGQRDPLPQQMNRLAALHQINRAATANLRLWDMLQQIATIVAEAAEADACSI
                     FLYDDGTGLLTLRAAIGLSPVSVGSATLRMGSGITGRAAVEQKLIAAPDAHRHESFMA
                     HPGIGDEIYTSQVSVPITTKAQNKLVGILNLNTIRRREFDGDELEFLETVAGELAISI
                     ENARQYSQTDERLRQKVAELGTLQRVSRILASTLDLADVLRLITEQAVELVHAEAAAI
                     FRIRETTNAGRRDVVPQIEHRVGPDLEMIDDSELNRIVSQVMQTGTAQRDEVTYVDGA
                     NTLFCLPLRSARETVGAICFRMRTGIELDDDTLGILQAFSDSAAMAIENAQLYQDAMH
                     SIQTQSALVQEMHHRVRNNLQTVAALLSLQLRSAEDAPWATEIREAISRIQSIAAVHD
                     LLSDERRLAGTTVDVIARMVAEDAHSTLIPPGIAVRFDIQTSQLTVASRQATILSLLI
                     NELTANAISHGFRNRSHG"
BASE COUNT          475 a          884 c          769 g          434 t
ORIGIN      
        1 ggatctgccg gaggtgatcg atctcgtcgc gacggcgccg gacgacaagg aaatccaggg
       61 ctggctcatt ccgccggtcg gcttcgatcc caccagcgac gccaggcatc ccgtcattct
      121 cgagattcac ggcggaccgc acgcaatgta cggctacgcg ctcttccacg agatgcagtt
      181 gatggcatcg aagggatatg cggtcgtctt cagcaaccct cgcggctcga acggctatgg
      241 cgaggagttc acgacctgca cccgcggcgt ctggggtgag gcggattcgc cagacatcct
      301 ggcggtgctt gagaaggcgc tcgaactccc ctggctcgac ccaacccgcc tcggaatcac
      361 cggcggctca tacggcggct acatgacgaa ctggatcatc ggtcacgatc agcgcttcaa
      421 ggctgccgtg acccagcgat gcgtgtcaaa cttctactcg ttcttcggaa cgagcgacat
      481 cggcttcgac ttcggcgcct tcgagttcga cggcctcccg tggaaggatg ccgcgaagct
      541 gctgaagcac tcgccgatca gctatgtcga tcagatcgaa acgccgctcc tgatccttca
      601 tagcgagcag gatctccgct gcccgatcga gcagtcggag gagatgttta ccgcgcttcg
      661 attcctggag aaggaagtcg ccttcgtgcg aattcccggc gagagccacg ggctctcccg
      721 caacggcacg ccgagccgcc gtctcgcgag actgcatcac ctgatcggat ggttcgactc
      781 gcacctgtag tcaacagtca tggggtcgct gatgaatgaa actctttccc tgagggaact
      841 tgccgatcgc ctcgcgaccc agctcgaccg ggcctactcc cccgcgcacg gccatgccgc
      901 gcgcgggggc gagcccctgg tcgaatcatt cgaagcattc gccggcgatg cccgggccgc
      961 gggtcttccg ctcgaagacg tgatgaccgc gggcatcgat accctgcaga cgttcttccg
     1021 gcttttcggc aacagtccgg cctccgaccc ggccaccatg cgtgccgccg gtgtggcgct
     1081 cgcctcggcg gcacggacgc acatcctccc gatccgaacg ttcgaggaca ccgcgaacgg
     1141 ccagcgagat ccgctcccac agcaaatgaa ccgcctcgcg gcgctccacc agatcaatcg
     1201 cgcggcaacg gcgaacctcc gcctctggga catgctgcag cagatcgcga cgatcgtcgc
     1261 cgaggccgcc gaggcggatg cctgctccat cttcctctac gacgacggaa cgggtctgct
     1321 cacgctgcgc gctgcgatcg gtctcagtcc cgtttccgtc ggttccgcga cgctgcgaat
     1381 gggctccggg atcaccggcc gcgcggccgt cgagcagaaa ctgatcgccg cgccggatgc
     1441 gcaccgccac gagagtttca tggcgcatcc gggaatcgga gatgagatct acacatcgca
     1501 ggtctcggtt ccgatcacga cgaaggcgca gaacaagctc gtcggcattc tcaatctcaa
     1561 cacgatccgg cgtcgcgagt tcgatggcga cgaactggag ttcctcgaga ccgtcgccgg
     1621 tgagcttgcg ataagcatcg aaaatgcccg ccagtacagc cagaccgacg aacggctccg
     1681 ccagaaggtc gccgagctcg gcaccctgca gcgcgtatcg cgaatcctcg cctcgaccct
     1741 cgacctcgcg gatgtcctcc gcctcatcac cgagcaggcc gtggaactgg tgcatgccga
     1801 ggcggcggcg atcttccgca tccgggagac gacgaacgcc ggacgtcgcg atgtcgtgcc
     1861 gcagatcgag caccgggtcg gtcccgatct cgagatgatc gatgacagcg agctcaatcg
     1921 catcgtctcc caggtcatgc agaccggaac cgcccaacgc gacgaagtga cctatgtcga
     1981 cggggcgaac acgctcttct gccttccgct ccgttcggcg cgcgagacgg tcggcgcgat
     2041 ctgcttccgg atgcggaccg ggatcgagct tgatgacgac acgctcggaa tcctccaggc
     2101 cttctccgac tcggcggcga tggcgatcga gaacgcccag ctctaccagg acgcgatgca
     2161 cagcatccag acccagtccg cactcgtgca ggagatgcat catcgcgtcc gcaacaacct
     2221 gcagaccgtc gctgccctgc tctcgcttca gttgcggtcc gcggaggatg ctccatgggc
     2281 gaccgagatt cgcgaggcga tcagccgcat ccagtcgatc gcggccgtgc acgatctgct
     2341 cagcgatgag cgccgcctcg cgggcacaac ggtcgacgtg atcgcacgga tggtcgccga
     2401 ggatgcgcac agcacgctga tcccgccggg catcgccgtc cgcttcgaca tccagaccag
     2461 ccaactcacc gtcgcgtcgc gccaggcaac catcctctcg ctgctcatca acgagttgac
     2521 ggcgaacgcg atcagccacg gtttccgcaa tcgctcccat gg
//
LOCUS       DASTMD010000431         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2541292, whole genome shotgun sequence.
ACCESSION   DASTMD010000431 DASTMD010000000
VERSION     DASTMD010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541292"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..1171
                     /locus_tag="VFQ54_06210"
     CDS             392..1171
                     /locus_tag="VFQ54_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0164622.1"
                     /translation="MTRIIAFFNQKGGTAKTTSTLNVAAALAERGHRVLMIDLDPQAS
                     LSMATGIDVAAIDLSVYDLLLDDAVGIAEVALPTTMAGVDIAPSHPDLAAAELELLNI
                     LERERQLEHRINAADLSAYDMVLIDSPPALNIISINILVAATELIIPIEPHPLSLMVL
                     RRLFETIQRIKRLNPGLQVLGFLPTKVHHSSRLAADMLLQLEEQFSDVPLLTSIPLSV
                     RGAESTAERTSILQYMPKSNVAAAYREIGTWLEEYPDRAHD"
     gene            1164..>1717
                     /locus_tag="VFQ54_06215"
     CDS             1164..>1717
                     /locus_tag="VFQ54_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU0164623.1"
                     /translation="MTERHDTPEQEQPAPRSRRRFTVDSLFSDTRPRSTGVTDLQDAK
                     EIRLDRIVPDPDQPRRMFDQDRLDELAASIRTDGVLQPIVVRYDEPEDRYIIVHGERR
                     WRASSLAGRETVPALIRDVPAERRLLQQLMENVVRDDLNAVDRANALRTLRSQLGDPA
                     WEVVAEAVGIKRSRLFQLLGTGKLS"
BASE COUNT          314 a          622 c          489 g          292 t
ORIGIN      
        1 ctgcgcgaat acctcgcatc gatccagccc cagcgctcgc ccgccttccg ccttcgcgag
       61 atcgccagca ccgtcctgat ggcggtcgcg ggctcgatca agccgaagca ggtagccacg
      121 gaacacgagc aggaactcta cgttccggaa acgagactcg cctcgtagcc cgaacgacgt
      181 tccagatgaa gaacccagag agaaccggga gcatcggatc agatccgtcg ctcccggttc
      241 attttgtccc cggatcccga tccgggtctc ctctcggctt gttctcggtc gctcgcggat
      301 gccacactga tcgcatgatt gactgcctgg aaccatcagg gccacgccga ctcacacaca
      361 tcttcagccc cagcgacggg acagtaaacg catgacacgc atcatcgcct tcttcaatca
      421 gaaaggcggc accgccaaga ccaccagcac cctcaatgtc gccgccgctc tcgccgagcg
      481 cggtcatcgc gtgctgatga tcgacctcga tccccaggca tcgctctcga tggccaccgg
      541 catcgacgtc gccgcgatcg atctctcggt ctacgacctc ctgctcgatg atgccgttgg
      601 tatcgcggaa gtcgcgcttc cgacgacgat ggcgggtgtc gacatcgcgc cgtcccaccc
      661 cgatctcgcg gcggcagagc tggagctgct caacatcctc gaacgcgagc gccagctgga
      721 acatcggatc aatgccgccg acctcagcgc ctatgacatg gtgctgatcg attcgccgcc
      781 ggcgctgaac atcatctcga tcaacatcct cgtcgccgcg accgagctga tcattccgat
      841 cgagccgcat ccgctctccc tgatggtgct tcgtcgcctc ttcgagacga tccagcgcat
      901 caagcgtctc aatcccggat tgcaggtgct gggcttcctg ccgacgaagg tgcaccattc
      961 gtcccgtctt gcggcggaca tgcttctcca gctcgaggaa cagttcagcg acgtgccgct
     1021 cctgacctcg atcccgctgt cggtccgcgg cgcggaatcg acggcggagc ggacgagcat
     1081 cctgcagtac atgccgaagt ccaacgtcgc cgccgcctac cgggagatcg gcacatggct
     1141 cgaggaatat ccggatcgtg cccatgactg agcgccacga caccccggaa caggagcagc
     1201 cggccccgcg atcgcgccgc cgcttcacgg ttgattcgct cttcagcgat acacgaccgc
     1261 gctcgaccgg cgtcaccgat ctccaggacg ccaaggagat ccggctcgac cggatcgtgc
     1321 ccgaccccga ccaaccccgg cggatgttcg atcaggaccg cctcgacgaa cttgccgcga
     1381 gcatccgcac ggatggtgtc ctccagccga tcgtcgtgcg atatgacgag ccggaggatc
     1441 gctacatcat cgtccacggt gagcgccgct ggcgagcatc ctcgctcgcc gggcgggaga
     1501 cggttccggc cctgatccgc gacgtgcccg cggagcgacg cctgctccag caactgatgg
     1561 agaacgtcgt tcgcgacgat ctcaacgccg tcgatcgcgc caacgccctg cgcacgctcc
     1621 gctcccagct cggcgatccc gcctgggagg tcgtcgccga agcggtcggg atcaagcgaa
     1681 gccgcctctt ccagctgctg ggcaccggca agctctc
//
LOCUS       DASTMD010000432         2728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3166767, whole genome shotgun sequence.
ACCESSION   DASTMD010000432 DASTMD010000000
VERSION     DASTMD010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3166767"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFQ54_06220"
     CDS             complement(<1..755)
                     /locus_tag="VFQ54_06220"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU0164624.1"
                     /translation="MLVVILGVLSGTVGGTLVDKFSVPGTESQKAIDALNANFPSASG
                     DSAQVVFKSDANITTDATAQKQINDFIAKAKTLPEVLSVSDPLAANSGTVSQNGQIAY
                     LTIQYDKPAQEIDTSAIDDLKDLVKQSSSATLETEVGGQVIQELPSTGVSELVGIAAA
                     IIVLLIMFGSLIAMGMPIITAGTGVAISTLIVTILASFLDMSTITTALLSMMGLGVGI
                     DYSLFMITRFREERSHGKRIEDSIVTAVDTAGRS"
     gene            1103..1714
                     /locus_tag="VFQ54_06225"
     CDS             1103..1714
                     /locus_tag="VFQ54_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0164625.1"
                     /translation="MSKKQEASSSRQGQEALLEVAQRLFTEHGYANVSMQQIAEEAGM
                     TKGAPYYYFQNKEALFARVSVRILSEIRESVMEASAIGGTFQERLIRCILTITRSTTG
                     NLEQWFADFPRVLSHEDLREIVAEAICAGDLAELLLPRFEEAAEKGEFTRVSPRTAAS
                     VFQQLLMMKMKDQANANRTADTPANYLETSTEELVDIFMHGIR"
     gene            complement(2181..>2728)
                     /locus_tag="VFQ54_06230"
     CDS             complement(2181..>2728)
                     /locus_tag="VFQ54_06230"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU0164626.1"
                     /translation="AARRGRRLDQMAMTVLAASALLASGDDIVAIAMFRRALELGQAE
                     EIVRPIIDDGRTLLPFLRRDAFTGDFAAPYLARLTSAAGLSQPASDGMSGRANVPGGV
                     KVASDPRTLESRIASLSARERQILAGIVDGLSNKEISKRLFIAEPTVRRHLTNAYAKL
                     GVRNRMQAVVLIRTTQAFPRG"
BASE COUNT          522 a          851 c          869 g          486 t
ORIGIN      
        1 gagcgtcccg ccgtatccac cgcggtgacg atcgaatcct cgatcctctt gccgtgggat
       61 cgctcctcgc ggaagcgggt gatcatgaag agcgagtagt cgatgccgac gccgagaccc
      121 atcatcgaga ggagcgccgt cgtgatcgtc gacatatcga ggaagctggc gaggatcgtc
      181 acgatcagcg tcgagatcgc gacgccggtg ccggcggtga tgatcggcat gcccatcgcg
      241 atcagggagc cgaacatgat cagcaggacg atgatcgcgg cggcgatacc gacaagctcg
      301 gaaacgccgg tcgacggcag ttcctggatg acctggccac cgacctccgt ctcgagggtc
      361 gcgctgctgc tctgcttgac gagatccttg agatcgtcga tcgccgacgt gtcgatctcc
      421 tgggccggct tgtcatactg gatcgtcagg taggcgatct ggccgttctg cgagaccgtg
      481 cccgaattcg cggcgagggg gtcgctcacc gagaggacct ccggcagcgt cttcgccttg
      541 gcgatgaagt cgttgatctg cttctgcgcc gtcgcatcgg tcgtgatgtt ggcatccgac
      601 ttgaagacga cctgcgcgct gtcacccgag gccgagggga agttcgcgtt cagggcatcg
      661 atcgccttct gcgactcggt gccgggaacg gagaacttgt cgacgagggt gccaccgacg
      721 gtacccgaga gcacgccgag aatcacgacg agcacgaccc atccaccgat gacctgccac
      781 ggatggtgcg ccgcaaagtg cgcccaacgg cggattggtc cggatctgga ctgagggagc
      841 gacgacatag ggagaccctt tctctgctgt cttgaggtac cgcccatggc gggtggccga
      901 ggggaacgct ggcgttggga gcggagtcgc catgtggacg ccatccccat cacgaggtat
      961 catactaaac gctcactcag tgaatcgcaa gggggttgcg aacacacaga cgattgcaaa
     1021 tgtcgcgtag caaggtgaag atgatgctat cggagcgcgg cgagccagac gcatatcagg
     1081 aagatggttt atggctgttt ccatgagtaa gaagcaggag gcatccagct cccgtcaggg
     1141 gcaggaggca ctcctcgaag ttgcacagcg gctcttcacc gagcatgggt atgccaacgt
     1201 atccatgcag cagatcgcgg aagaagccgg catgaccaag ggcgcaccct attattactt
     1261 ccagaataag gaagcgctat ttgcccgggt atcggtccgg atcctctccg agatcaggga
     1321 gagcgtgatg gaagccagcg ccattggggg tacctttcag gaacgcctga ttcgctgcat
     1381 cctcacgatt acccggtcga ccaccggcaa tctcgagcaa tggttcgctg attttccgcg
     1441 cgttctcagt cacgaggatc tgcgagagat cgtggcggaa gcgatctgtg ccggtgatct
     1501 tgccgaactc ctgctccccc gattcgagga agcggccgag aagggcgagt tcacacgcgt
     1561 ctcgccgcgg acggcggcga gtgtcttcca gcaactcctg atgatgaaga tgaaggacca
     1621 ggcaaacgcc aaccggactg cggatactcc tgcgaactat ctggagacaa gcaccgagga
     1681 actcgtcgat attttcatgc acggaattcg ttgatcgtcg tctttttcga ctgccgcgat
     1741 tccagaacgc atcgctgccg catgactcat cggctccccc ggcgatgaca gccccgccag
     1801 ggacaccgca agcaaacgcg aggcgggaca ggccgtccgg acctgcggtc cggcagcggc
     1861 ggtcccgccc tacgaacgac cgggcgcccg acgcgcgatt cgcatctgta ggcaagcgtc
     1921 ctcactcttg cggggatgcg aaatcacgga ccgtcgccac gttgaacctg atcggggttt
     1981 gtcgggcaag accgtgtgtc ttgcccagga ctagagcaag gggcggaaga cgtgcctgcc
     2041 gctccccttt ctggtcgtca tcctgagctt gtcgaaggat ccccgcgcga agcgcatccg
     2101 gcgccgccgg actccaccac ggtcgtttgg catgccacgt ccggttgggc accttcgccg
     2161 ggctcacgcc tctctgcagg ctagccccgg ggaaacgcct gcgtcgtacg gatcaggacc
     2221 accgcctgca tccggttccg cacaccaagc ttggcgtacg cgttcgtcag gtgacgccgg
     2281 accgtcggct cggcaatgaa gagccgcttc gagatctcct tgttcgagag accatcgaca
     2341 atccccgcca ggatctggcg ctcccgcgca ctcagcgatg cgatccggct ctcgagcgtt
     2401 cgtggatcgg acgcgacctt caccccgccc ggtacgttgg cccgtccact catcccgtcc
     2461 gatgccggct gggagagccc ggcggcactc gtgagcctgg cgagatacgg cgcggcgaag
     2521 tccccggtga acgcatcgcg acggaggaac gggagcagtg tccggccatc atcgatgatc
     2581 ggccggacga tctcctcggc ctgtcccagt tcgagcgccc ggcgaaacat cgcgatcgcc
     2641 acgatgtcgt caccggaggc aagcagcgcc gacgccgcga ggacggtcat cgccatctgg
     2701 tcgagccggc gtccgcggcg cgctgcct
//
LOCUS       DASTMD010000433         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1470781, whole genome shotgun sequence.
ACCESSION   DASTMD010000433 DASTMD010000000
VERSION     DASTMD010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470781"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VFQ54_06235"
     CDS             complement(<1..595)
                     /locus_tag="VFQ54_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164627.1"
                     /translation="MRRYSSRGVLVFALAFGMLAAIVATGAAPIVPDVVAANPNCSGL
                     IVNGGFEQGTPGSGANATGWTGSYWLTNAGDQHSGSQYVYLTESYPTISQVLITTGLP
                     AGTIITVTFYQDSGIHGTLGTTSQDFPSHGAYNYGQETISYTLVTATPSVALTFTNID
                     LPFLDDVSATCVGPTPTPTNTPTVTPTNTPTATPTNTP"
     gene            complement(770..1690)
                     /locus_tag="VFQ54_06240"
     CDS             complement(770..1690)
                     /locus_tag="VFQ54_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164628.1"
                     /translation="MPESRPDLSTLPRYERPKEATPAPVAFGDGEPIAYADLARFLET
                     STLTVGGRDLLRGLLRLGRAAIFAGGGAGIEYSSLLKTATRPEYIGAHVQEVAAHLRA
                     CGVDVLVVPGMSGYPVGSMYSIASGIPAVLLKKQKLAEHAIGIGYPPGAFLIPSYTGD
                     GDVLMSADPASMQDIVDEILEPQFAAQTEGERIVLRIRVAGADDIIDKATMSQAVSDS
                     ALIVGKTAIANRVARYRRETGDERPVEEIVEVVAWVTPLIKGYNRPHEHLQRWFGVTP
                     FAGINVTSVHLEPSAIGVEGIGIVAFAPER"
     gene            complement(1731..2675)
                     /locus_tag="VFQ54_06245"
     CDS             complement(1731..2675)
                     /locus_tag="VFQ54_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164629.1"
                     /translation="MGDVWTIGFLTSVLAAAVASGAALLYAALGEVFAERAGVLNLGV
                     EGMMLMGALSGFAFCYWSGSAWLGLLGAIVAGALMSVIHAVVTISLQANQVVSGLALT
                     LFGQGLSAYLGTSLVGKPAPESFGKLAIPLLSDIPRLGTILFRQDALVYIGYLLVPLL
                     WWYVYRTRPGLNLRAVGENPATVDAMGINVVGLRYGYVIVGGAFAGVGGAAISLATNP
                     GWSENMTAGRGWIAVALVIFGVWNPARAAAGALLFGAVEAANFRLQAVNVPVSSFFLN
                     MLPYIFTIVVLVLATRQSRARRIGAPAALGQAYAREER"
     gene            complement(2675..>3494)
                     /locus_tag="VFQ54_06250"
     CDS             complement(2675..>3494)
                     /locus_tag="VFQ54_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164630.1"
                     /translation="WNIGAEGQFYFGAIFATWAAIYGFKDSGPWVVIPAMAIAGMIGG
                     ALWAAISGILRGYLGVNETITTLMLNYVAILFSDYLVHGPWRDPQGYGFPGTETFSDA
                     SYLPTWGTTRVHLGLAFGLAAAVVVYVVLRRTRWGYEIGVTGASERVARYAGMRTARN
                     IVLVLMLSGALAGLAGMGEVAGINHSLQSDLSPGYGYTAIIVAWLGRLHPLGIVVVSF
                     LLAGLLVGGDQLQTSMGLPSAIAPMMQGMILFCLLGGDFLARYRVVRVQPKEAV"
BASE COUNT          691 a         1180 c         1082 g          541 t
ORIGIN      
        1 tcggcgtatt ggtcggcgtc gcagttggcg tattggttgg ggtcacggtc ggcgtgttgg
       61 ttggcgtcgg cgtggggccc acgcaggttg cgctgacgtc gtcaagaaag ggcagatcga
      121 tgttggtgaa cgtcaacgcg accgacgggg tcgcggtcac caaggtatag ctgatcgttt
      181 cctgcccgta gttgtatgcc ccgtggctcg gaaagtcctg actggtcgtt cccaaggtgc
      241 cgtgaatccc gctgtcctgg tagaacgtca ccgtgatgat cgtcccagcc ggcaagccgg
      301 tggtgatcag cacctgagag atcgtggggt agctctcagt caaatagaca tactgactgc
      361 cggagtgctg atcaccagcg tttgtgagcc aatagctccc ggtccatccc gtcgcgttgg
      421 caccggaccc aggcgtgcct tgctcaaagc cgccgttaac gatcaagccc gagcaattcg
      481 ggttggcggc gacgacatcg ggcacgatcg gtgcagcgcc ggtcgccacg atcgcggcca
      541 gcatgccgaa cgcgagcgcg aacacgagga ccccgcgtga cgaatagcga cgcatccgct
      601 gactccggtt ccacgttcat gggcaatgtt cactttttct ggacccttgg gcgaactcta
      661 gccggtgaac gctggcgccg tcaatctgtc gcgacttctg gatgctgccg aagcaacgcg
      721 cggtcgcaac ccatgttttt caggaaagag catctgatcg ttatgtgtgc tatctctccg
      781 gagcgaaagc aacgatgccg ataccctcga ccccgatcgc cgacggctcg agatggacgc
      841 tcgtcacgtt gatcccggca aacggcgtca cgccaaacca gcgctgcagg tgctcgtgcg
      901 gtcggttgta gcccttgatc agcggcgtca cccaggcaac cacttccacg atctcctcga
      961 ccggccgctc atccccggtt tcccggcggt agcgagcgac gcgattcgcg atcgccgtct
     1021 tccccacgat cagcgcgctg tcgctgaccg cctggctcat cgtcgccttg tcgatgatgt
     1081 cgtccgcgcc cgcgacgcga atccggagca caatccgctc gccctccgtt tgcgcagcga
     1141 attgcggctc caggatctcg tcgacgatgt cctgcatcga ggcaggatcg gcgctcatca
     1201 ggacatcgcc atcacccgta tacgagggga tcaggaatgc tcccggcggg tagccaatcc
     1261 cgatcgcatg ttccgccagc ttctgcttct tgagcaggac agccggaatg cccgaagcga
     1321 tcgagtacat cgacccgacc gggtagccgg acatcccggg cacgacgaga acgtccaccc
     1381 cgcacgcgcg aagatgcgcc gcgacctcct ggacgtgggc gccgatgtac tcgggccgcg
     1441 tcgccgtctt cagcaggctg ctatattcga tccccgcccc gccgccggcg aagatcgccg
     1501 cccgcccgag ccgaagcagc ccacgtagga gatcgcgacc gcccaccgtc aatgtcgatg
     1561 tttcgaggaa ccttgcgaga tcggcgtagg cgatcggctc gccgtcgccg aaggcaaccg
     1621 gcgcgggcgt ggcctccttc ggccgctcgt agcgcgggag cgtcgaaaga tcgggacggg
     1681 actcgggcat aacgaaggta cctgttccat tcgaatcgca gaaagcggcg ctagcgttcc
     1741 tcgcgggcgt acgcctggcc aagtgcggcc ggtgccccga tccgacgcgc tcgcgactgc
     1801 cgcgtggcga ggacgaggac aacgatcgtg aaaatatacg gcaacatgtt gaggaagaag
     1861 gacgaaaccg gaacattcac cgcctgaagc cggaaattcg ccgcctcgac cgcgccgaag
     1921 agcaacgcac cggcggcagc gcgcgccgga ttccagacgc cgaagatgac gagcgcgacc
     1981 gcgatccagc cgcgaccagc ggtcatgttc tcgctccagc ccggattcgt cgcgagcgag
     2041 atcgccgcgc cgccgacgcc cgcgaacgcg ccaccgacga tcacgtagcc gtatcgcagc
     2101 ccgacgacgt tgatccccat cgcatccacc gtcgccgggt tctcgccgac ggcgcgaaga
     2161 ttcaggccgg ggcgggtccg gtagacgtac caccagagca gtggaacgag caggtagccg
     2221 atgtacacga gtgcgtcctg ccggaagaga atcgtgccga gacgcgggat atcactgagg
     2281 agcggaatcg ccagcttgcc gaacgactcc ggcgccggtt tcccgaccag cgacgtcccg
     2341 agataggcgg agagcccctg gccgaagagg gtcagcgcca ggccactcac gacctggttc
     2401 gcctgcaacg agatcgtgac gacggcatgg atgaccgaca tcagcgcccc ggcgacgatc
     2461 gcgccaagca gcccgagcca ggcgctgccc gaccagtaac agaaggcgaa cccggagagc
     2521 gcgcccatca gcatcatgcc ctcgacacca aggttgagga cgcccgcccg ctcggcgaag
     2581 acctcaccga gcgcggcata gagcaacgcc gctccggatg caacggccgc cgccagcacc
     2641 gaggtcagga aaccgatcgt ccagacatca cccatcagac ggcttccttc ggctgaacgc
     2701 gcaccacccg gtaacgtgcg aggaaatctc caccaagcag gcagaagagg atcatcccct
     2761 gcatcatcgg ggcgatggcc gacggcaatc ccatcgaggt ctgcaactgg tctcccccaa
     2821 ccagcaaccc cgcgaggaga aaggagacga cgacgatccc gagggggtgc aatcgcccaa
     2881 gccacgccac gatgattgcc gtgtacccgt accccggtga gagatcgctc tgcagagagt
     2941 gattgatgcc cgcgacttcg cccattccgg cgaggccagc aagcgcgccg ctcagcatca
     3001 ggaccagcac gatgttgcgc gccgtccgca tcccggcata cctggcgact cgctcgctcg
     3061 cccccgtgac accgatctcg tatccccacc gcgtccgccg cagaacgacg tagacaacga
     3121 ccgccgccgc cagaccgaac gcgagaccga gatgcacgcg cgtcgtcccc caggtcggaa
     3181 gatagctcgc gtcggaaaac gtctcggttc cggggaagcc atagccctgc gggtcccgcc
     3241 agggcccgtg gaccaggtag tccgagaaca ggatcgcgac atagttgagc atcagcgtcg
     3301 tgatcgtctc gttcaccccg aggtagccgc gaaggatgcc ggagatcgcg gcccagagcg
     3361 cgccgccgat catcccggcg atcgccatcg cggggatcac gacccaggga ccggaatcct
     3421 tgaaaccgta gatcgccgcc caagtcgcga aaatcgcgcc gaagtagaac tggccctccg
     3481 cgccgatgtt ccag
//
LOCUS       DASTMD010000434         4083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_308726, whole genome shotgun sequence.
ACCESSION   DASTMD010000434 DASTMD010000000
VERSION     DASTMD010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308726"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="VFQ54_06255"
     CDS             complement(<1..1198)
                     /locus_tag="VFQ54_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164631.1"
                     /translation="MVADQVTQSIEFSGSAKSKKLASLAFDVMRGRGMFMSDNSSIRV
                     PFDAVAAFLNAQAEASVSDVEAAIAANAAVFSTEVVDDVTFVVTTRLGRAPHGPVAEP
                     NHTFQSRFLTPEPKPIAPVAPRRERARVDPSWASFQLSDYAHLDEEEDEPEDEVLETT
                     PVTIGADDIDLIFEPLESETVAASAAAATETAPVIEAEPVAAAEVVAAPVEVTPPAAA
                     TPAVVDLDRYSEDDLAAAIHDRLAADVHVAQFGDQWMSEDRVPRLSRGDLRRIKDYIE
                     EQEQPLTDAMLVQDILGIRANAVDFGLMQFAVNFRLSREHRDFDFVGTNEQRFWTTTN
                     LAPIGTTRRKPNEIGTDFRFLIDEAADQVVEPRSVKSIDHVVTFYEYNLGLLPYDLDM
                     QRLFPRP"
     gene            complement(1324..1461)
                     /locus_tag="VFQ54_06260"
     CDS             complement(1324..1461)
                     /locus_tag="VFQ54_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164632.1"
                     /translation="MALQSGTLRLTDEDVMYLLTLLRNASTPLTTAQLIDALKQRSGR
                     S"
     gene            1838..3049
                     /locus_tag="VFQ54_06265"
     CDS             1838..3049
                     /locus_tag="VFQ54_06265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164633.1"
                     /translation="MTIASGHFTVDMYSGMIPVLFPALMHHYGVSLGEVALVSLAYTG
                     CSSVSQPLFGWIADRFGTRYIGFTQMWSALLFSTVGFAPNFVLLVLLAGLAGFGSGAF
                     HPMGAMNASAVIKPGQGNMSMSVYVSGGTIGFALGPLIGAGLVYLFGLHGTIVMLIPG
                     LAIGSWLIFAMKDVAVTGRPRRGGSRAHPAVAAVAIPVGLISIVVAMMMLRQVPVVGI
                     ESFLPAWYSDLGYSSGFYSSLATTMVLASAAGALGVGGFADRYGRRAVMIATIVLTIP
                     VVWAFITFPGPWAFVFGAMIGFLASSTGPLLLLMAQQLMTGNAGMASGLILGLGFVSG
                     GIATPILGSIADATSMTTALYCLLGIVILSIAVAWLLPSESTIRGITERQLQASSLAS
                     ATERGEAAGVA"
     gene            3270..3707
                     /locus_tag="VFQ54_06270"
     CDS             3270..3707
                     /locus_tag="VFQ54_06270"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEU0164634.1"
                     /translation="MFDKLKDAVSDLIGKNDDAEKAVDTTTSAADDVKSTLGQTIDDV
                     KEGGLSADSLKSAAGDLGGLKDRLTGDANSLSGVVDQIGGLGELTQFVQGIDFPINKD
                     ELISQLKQNGSFNDIVEKLEGVAKEQFGSKSDLLDTVKGFISK"
     gene            3880..>4083
                     /locus_tag="VFQ54_06275"
     CDS             3880..>4083
                     /locus_tag="VFQ54_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU0164635.1"
                     /translation="MATLERPNFSSRLSALAQAPGVYLMKDAEGTTIYVGNAIALRNR
                     VRSYFQSQRNKDPKTRELVAHIVD"
BASE COUNT          696 a         1248 c         1318 g          821 t
ORIGIN      
        1 ccggccgtgg gaagagtcgc tgcatgtcga ggtcgtaggg aagcaggccg aggttgtact
       61 cgtagaaggt cacgacatgg tcgatcgact tcacggagcg cggctcgacg acctggtcgg
      121 cggcctcatc gatcaggaaa cggaagtcgg ttccgatctc gttcggcttg cggcgcgtcg
      181 tgccgatcgg cgccagattc gtcgtggtcc agaaccgctg ctcgttcgtg ccgacgaagt
      241 cgaagtcgcg atgctcgcgg gagagccgga agttgacggc gaactgcata aggccgaaat
      301 cgacggcatt cgcgcgaata ccgaggatgt cctggacgag catcgcatcc gtcagcggct
      361 gctcctgctc ctcgatgtaa tccttgatcc ggcggaggtc accacgactc aggcgtggca
      421 cgcgatcctc ggacatccac tgatcgccga actgggcgac gtggacatcc gccgcgagac
      481 gatcgtggat cgccgcggcg aggtcgtcct cggagtagcg atcgagatcg accacggccg
      541 gtgtcgcggc agcgggggga gtgacctcca ccggcgcggc caccacttcg gccgcggcga
      601 ccggctcggc ctcgatgacc ggggccgtct cggttgccgc ggcggcggaa gccgccaccg
      661 tctcgctctc gagtggctcg aagatcagat cgatatcatc cgccccgatc gtaaccgggg
      721 tcgtttccag gacctcgtct tccggctcgt cctcttcctc atcgagatga gcgtaatccg
      781 agagctggaa gctcgcccac gacggatcga cacgggcccg ctcgcgtcga ggcgcgacgg
      841 gggcgatcgg cttcggctcc ggcgtcagga aacgcgactg gaacgtgtgg ttcggctcgg
      901 cgacggggcc gtgcggcgcc cgccccaacc tcgtcgtgac gacgaaggtc acgtcgtcca
      961 cgacttccgt gctgaacacg gcggcattcg cggcgatcgc agcttcgaca tcggagacgg
     1021 aggcctccgc ctgggcgttg aggaaggcgg cgacggcgtc aaacggaacg cggatcgacg
     1081 agttgtcgga catgaacatg ccgcgcccac gcatcacatc gaatgcgagc gatgccagtt
     1141 tctttgattt ggccgagccc gagaattcga tgctctgcgt tacctgatcg gccaccatgg
     1201 ctgaacgttc tccttgcgaa gggcttggcg tgttgctggg gtaggacgtg cgcgatgttg
     1261 atttcgagaa cgcgtcctgt cttccccggc tgattcggaa ccggacggcg tttcccggag
     1321 gatctacgag cgaccggacc gctgcttcag ggcatcgatg agctgcgccg tcgtgagcgg
     1381 cgtggatgca ttgcgcagga gcgtgaggag gtacatcaca tcttcatctg taagtcgcag
     1441 cgtgccagat tgcagagcca tccgagtaaa catctcctta agaggtcggg ttggaacacg
     1501 aaacgggctg ctaagcaacc ctctcccaaa cttgaagagt atagaggatc gacggcttcc
     1561 cgtgcggagg ctctcgcggc aggcgcctcg aacggatcac accgggcgaa ccaggccatt
     1621 tcaatcccgg gcggagaccg tagaatggac gggctctcgc aacgtccgcc aaggactcgg
     1681 gagcgcatcg ttcccggatg cgcgccgtca gagcccggca acaagaacct tctccatcgc
     1741 gaaggtgaca cactcgctgt gtgcctcgtc agaaatggtg tcgatcaacg tggcttcgtc
     1801 cgcctctccc attgatgtgc tccggaatcg accgctgatg acgatcgcga gcggtcactt
     1861 cacggtcgac atgtattcgg gcatgatccc ggtcctcttc ccggccctca tgcatcacta
     1921 cggcgtctcg ctgggtgagg tcgctctcgt atcgctcgcg tacaccggct gttcgtccgt
     1981 ctcccagccg ctctttggct ggatcgcgga tcgtttcggg acgcgctaca tcgggttcac
     2041 ccagatgtgg tcggcgctgc tcttctcgac ggttggcttc gccccgaact tcgtgctcct
     2101 cgtgctgctg gcgggactgg ccggattcgg ttccggtgca tttcacccga tgggtgcgat
     2161 gaacgccagt gccgtgatca aacccggcca gggcaacatg tcgatgtccg tctacgtctc
     2221 cggtggcacg atcgggttcg cgctcggtcc cctgatcggc gccggcctgg tctatctgtt
     2281 cggacttcac ggcacgatcg tgatgttgat cccgggactc gcgatcggat cgtggctgat
     2341 cttcgcgatg aaggatgtcg ccgttaccgg caggccgcgt cgcggtggtt cgcgggcgca
     2401 tccggcggtt gccgccgtcg cgatcccggt cggtctcatc tccatcgtcg tggcgatgat
     2461 gatgcttcga caggtcccgg tcgtcggcat cgaatcattc ctgccggcgt ggtattcgga
     2521 tcttgggtac agctcgggct tctatagctc gctggcgacg acgatggtcc ttgccagtgc
     2581 cgccggtgcg ttgggggttg gtggtttcgc cgatcgctat ggccgccgtg ccgtgatgat
     2641 cgcgacgatc gtcctgacga tcccggtagt atgggctttc atcaccttcc ccggtccgtg
     2701 ggcgttcgtc ttcggcgcga tgatcgggtt tctcgcttcc tccacggggc cgctgctcct
     2761 gttgatggcg cagcagctga tgacgggaaa cgccgggatg gcatcgggcc tcattctcgg
     2821 gctgggattc gtgtcgggtg gcatcgcgac gccgatcctc gggagcattg ccgacgcgac
     2881 gtcgatgacg accgcgctct attgcctgct cggaatcgtg atcctgtcga tcgccgtcgc
     2941 ctggctcctt ccgtccgaat cgacgattcg tgggatcacg gagcgccagc tccaggcatc
     3001 cagcctcgct tccgccaccg agcgcgggga ggcggccggg gtcgcctgac cagcgaacga
     3061 tgctggttgg cgggccgttc catctcgtga gagggatatc gtgggggaag ctgccctcgg
     3121 ccctgttatt gaggtgaccc gggaggtctt cgtggttgtt ctctcgcaaa cttgccgcag
     3181 tagtttgaga aaagtacaat tcgtagagtt cttttcgcga atctgtttcg ctgcgcgtcg
     3241 ggtgacgtgc gtacgggaag gggcccagga tgtttgacaa gctcaaggac gcggtgtcgg
     3301 atctcatcgg caagaatgat gatgccgaaa aggccgttga cacgacgaca tccgctgcgg
     3361 atgacgtcaa gtcgacgctc ggccagacca ttgatgatgt caaggaaggc ggcctctccg
     3421 ccgactccct caagtcggcc gccggcgacc tcggcggact caaggatcgc ctgacgggcg
     3481 atgcgaacag cctcagtggc gtcgtcgacc agatcggcgg cctcggcgag ttgacgcagt
     3541 tcgtccaggg catcgacttc ccgatcaaca aggacgagtt gatctcgcag ttgaagcaga
     3601 acgggtcgtt caacgatatc gtcgagaagc tcgagggcgt ggccaaggag cagttcggca
     3661 gcaagtccga tctcctggat accgtcaagg gcttcatctc gaagtaatcg tcgattctgg
     3721 taacccgaag ccaggcgtcc gccctgtcgg ggcgggcgcc tctctgtgtc cggagaacga
     3781 tcctttgtgc gcggcgcatc attgcgcccc gcaactggcg cgaatcgagg gcattcgcgg
     3841 aggtcttgcc cggggcaacc gtctatactt gtacgtgcta tggcaacgct agaacgaccc
     3901 aacttttcat cccgactttc cgcactcgca caggctcccg gcgtctatct catgaaagac
     3961 gcggagggca cgaccatcta tgtcggcaat gcaatcgccc tgcgcaaccg cgtccgatcc
     4021 tacttccagt cacagcggaa caaggatccg aagacgcgcg aactggtcgc gcacatcgtc
     4081 gat
//
LOCUS       DASTMD010000435         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3076193, whole genome shotgun sequence.
ACCESSION   DASTMD010000435 DASTMD010000000
VERSION     DASTMD010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3076193"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VFQ54_06280"
     CDS             <1..428
                     /locus_tag="VFQ54_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0164636.1"
                     /translation="LPLLREAALAAPSSDILTISSTAGRVAYEGGGGYTAAKHGTTDI
                     IETLRLELAGEPIRVTEVAPGMVQTPEFSLNRFGGDQAAADKVYQDVQEPLTEKDVAL
                     VIVTMLELPKHINLDLVVMRPVAQAAQHKLVRGPLTPKL"
     gene            441..830
                     /locus_tag="VFQ54_06285"
     CDS             441..830
                     /locus_tag="VFQ54_06285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU0164637.1"
                     /translation="MPTFFRSTTFTQTQRDYIAGQRLGRLATIDANGNPQNNPVGFRL
                     NTELGTIDIGGHGMGNSKKFRNVLANPKVAFVVDDVATEPRQVRFVEIRGTAEQVTGE
                     EPIFPGQDPEFIRVHPTLIISFGLGED"
     gene            1427..2404
                     /locus_tag="VFQ54_06290"
     CDS             1427..2404
                     /locus_tag="VFQ54_06290"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEU0164638.1"
                     /translation="MQRLPFAKPGQFYKGNLHTHSTNSDGNLTPAEVIAEYKQRGYDF
                     IALTDHFLPETYFRPESDPASFITISDTSDLRSDDFTTIFGAEIHGPGMSNGELWHLV
                     AVGLPLDFPRWTPEETGDALAKRAVEAGAFVGIAHPHWNSLTQEDARKVAPFVHSVEV
                     YNHACAQIDRADGLYMLDALLEDGFHLSANAADDAHFKTPVGAIGEAHPEAFGGWVQV
                     KAESLEPDALLAALKAGEFYASTGPELVNIEIDAATDEIAIETSPVNRIMVTGLGPAS
                     ARVIGDDLTSARFPLEKFRKAGFVRVAAIDADGKRAWSNPIWLDAAPVA"
     gene            complement(2485..>2785)
                     /locus_tag="VFQ54_06295"
     CDS             complement(2485..>2785)
                     /locus_tag="VFQ54_06295"
                     /inference="COORDINATES: protein motif:HMM:NF025636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HEU0164639.1"
                     /translation="DLYLRLIGHGGSAYFIRRFQAGNTEILHEYWRQTHPGGTGHVIP
                     DEARARFGASATLGLFGYWLEHGRHEQAEDIAAWYWRLMRPILFEDIDDDSPSGR"
BASE COUNT          522 a          927 c          880 g          456 t
ORIGIN      
        1 tgctgccgct gttgcgggag gcggcgttgg ccgcaccgag ttcggacatc ctgacgatct
       61 cgtcgaccgc gggtcgcgtc gcctacgagg gtgggggcgg ctacaccgcc gccaagcacg
      121 gcacgacgga catcatcgag acgcttcgcc tcgaactggc gggcgagccg atccgcgtaa
      181 cggaggtcgc gccgggcatg gtgcagacgc cggagttctc gctgaatcgc ttcggtggcg
      241 atcaggcggc ggcggacaag gtctaccagg acgtgcagga gccgttgacg gagaaggatg
      301 tcgcgctggt gatcgtgacg atgctcgagt tgccgaagca catcaatctc gatctcgtcg
      361 tgatgcgacc ggtcgcgcag gcggcgcagc acaagctggt gcgtggcccg ctgacgccga
      421 agctctaaag gaggcaagac atgccgacgt tcttccgaag caccacattc acccagaccc
      481 agcgcgacta catcgcgggc cagcgactcg gtcgcctcgc gacgatcgat gccaacggca
      541 atccgcagaa caacccggtc ggcttcaggc tcaacaccga gctcgggacg atcgacatcg
      601 gcggacatgg gatggggaac agcaagaagt tccgcaacgt gctcgcaaat ccgaaggttg
      661 ccttcgtcgt cgacgatgtc gcgacggagc cgcgtcaggt gcgcttcgtg gagattcgtg
      721 gcacggcgga acaggtcacc ggcgaggagc cgatctttcc gggacaggat cccgaattca
      781 tccgcgttca cccgaccctg atcatcagct tcggtctcgg cgaggactga catccttgcg
      841 tcgcatgccg taatcccgta gcgggccacc tcgtgtggcc catcgggcgt agcccgatat
      901 gatcgtcggc acccctcagt gaaacccaga accatcgaca cgtttggtgc tgccggggtt
      961 ggtagggcgg ggtttcatac cccgccgcaa agcggattcc tgagaaacac gcctcgcctc
     1021 gatgcgtcct ccaatgggat gatccatgca acgcgtttcc gtgaaaccgg gactgggaaa
     1081 gcgatgcgaa cgtgcgggca acgcgcagca catcacaccg ctcgcccacg caggcggatc
     1141 ggctgcgcca atccgaggcg accacatggg tcgccgggac gggtttcagc gaaggtgccg
     1201 tgtgccggac gttcatatcg acctgcggtc gatgggccac acggggtggc ccgctacggg
     1261 attacggaca atcgctccgg gcgttcggcc aggaatcatt gcatcgaaac ctatgcacga
     1321 aggtgcattc accttcagtt gatgtttggt ccgaaacgcc cttccagaat gttccctggt
     1381 ttcgttttga cgcctccacg cgcattcacg acgagggaac gcatccgtgc aacgcctgcc
     1441 attcgccaag cctggccagt tctacaaggg aaatctccac acccactcta ccaactccga
     1501 cggcaacctg acgccggccg aggtgatcgc cgaatacaag cagcgcggct acgacttcat
     1561 cgcgctgacc gatcacttcc tgcccgagac ctacttccgc ccggagtcgg atcccgccag
     1621 cttcatcacg atcagcgaca cgagcgacct gcgcagcgat gacttcacga cgatcttcgg
     1681 cgccgagatc cacggtcccg ggatgtcgaa cggcgagctc tggcacctcg tcgcggtcgg
     1741 gttgccactc gatttcccga ggtggacgcc ggaggagacg ggcgacgcgc tcgcgaagcg
     1801 cgccgtcgag gcaggcgcct tcgtcggcat cgcgcatccg cactggaact cgctgaccca
     1861 ggaagatgcg cgcaaggtcg cgcccttcgt ccactcggtc gaggtctaca accacgcctg
     1921 cgcgcagatc gatcgcgcgg atgggctcta catgctcgac gcactgctcg aggatggctt
     1981 ccacctgagc gcgaatgcgg ccgacgacgc tcacttcaag acccccgtcg gcgcgatcgg
     2041 cgaggcgcac ccggaggcct tcggcggctg ggtccaggtc aaggcggaat cactggagcc
     2101 ggacgcgctg ctcgccgcac tcaaggcggg tgagttctac gcctcgaccg gtcccgaact
     2161 cgtcaacatc gagatcgacg cggcgaccga cgagatcgcg atcgagacct cgccggtgaa
     2221 ccggatcatg gtcaccggtc tcggcccggc gagcgcgcgg gtcatcggtg acgacctcac
     2281 ctcggcccgc ttcccgctcg agaagttccg caaggccggc ttcgtgcgcg tcgccgcgat
     2341 cgacgccgat ggcaagcgcg cctggtcgaa tccgatctgg ctggacgcgg ccccggtcgc
     2401 gtaatcatcg cgacgcagac aggggactgg ggatcgcgcg ttgcgatccc cagtcccctg
     2461 tgacggcatc gacggagcca cggatcaacg acccgaaggc gaatcgtcgt cgatatcctc
     2521 gaacaggatc ggccgcatca accgccagta ccacgcggcg atatcctccg cctgctcgtg
     2581 ccgcccatgt tccagccagt acccgaagag cccgagtgtg gccgacgccc cgaagcgggc
     2641 gcgcgcctca tccggaatca cgtgtcccgt tccgcccgga tgcgtctggc gccagtactc
     2701 atgcaggatc tcggtattcc ccgcctggaa gcggcggatg aaatacgccg agccgccgtg
     2761 tccgatcagc cgcaggtaga gatcg
//
LOCUS       DASTMD010000436         3764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2899223, whole genome shotgun sequence.
ACCESSION   DASTMD010000436 DASTMD010000000
VERSION     DASTMD010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2899223"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFQ54_06300"
     CDS             complement(<1..237)
                     /locus_tag="VFQ54_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEU0164640.1"
                     /translation="MESTYQIEEIPIEDLGQTWGPRPTERMVEHVKRHGVLVPILVTR
                     TPDEDGVLALRVIDGNRRINAASDAGLATVPAIIL"
     gene            406..747
                     /locus_tag="VFQ54_06305"
     CDS             406..747
                     /locus_tag="VFQ54_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEU0164641.1"
                     /translation="MTESGVKTELPPIIARHLPEIVALAKSYGVERLEVFGSVMTDRY
                     DPLLSDVDFLVKYPRGYDYGPFLKHYQRFESDLAELLHSPVDLVMDSQSLRDRFRRNI
                     AGTRTVLYDAR"
     gene            737..1081
                     /locus_tag="VFQ54_06310"
     CDS             737..1081
                     /locus_tag="VFQ54_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEU0164642.1"
                     /translation="MLDKTRDRLEDVLAAAAYIASVAESMSEAEYVANDMARFAIERN
                     LITIGEALARIRSTERSIADLISTSEEVVGMRNSMVHEYKKTDDVAVWQTVTVFLPAL
                     VEEVEAILDAQS"
     gene            1092..1874
                     /locus_tag="VFQ54_06315"
     CDS             1092..1874
                     /locus_tag="VFQ54_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0164643.1"
                     /translation="MRNPFLSVAAAQVAPDFGNVEANVQIATDFLREAAGQEIDLLVF
                     PELSLIGYDLGQLDRPEVWITADDERLAPLRQVVVETGIMTIVGGPYRDEDGVPRLSS
                     FVLHPDGSREIVFKMHIPGDEQSHFIAGPYPPKILELNGWNVAVAICFDAANPQHAMA
                     ARAEGADVYVDSALYVTSEIRRMDLHFASRAMDHRMFSLLANHAETGPGWESCGRSGT
                     WGPDGEVRVTSGVEPRLVIDRLPYADLERYRLRPVHAGTSAD"
     gene            complement(2286..2576)
                     /locus_tag="VFQ54_06320"
     CDS             complement(2286..2576)
                     /locus_tag="VFQ54_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU0164644.1"
                     /translation="MSDFFVYILANSKRTIYIGVTNSLERRLWEHRGGITTGFASEHG
                     CDQLVYFEDFASPRDAIAREKQVKGWRREKKIALIEAANPDWLDLGEDWRET"
     gene            complement(2870..3688)
                     /locus_tag="VFQ54_06325"
     CDS             complement(2870..3688)
                     /locus_tag="VFQ54_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0164645.1"
                     /translation="MKLGIFETVLARPTFAATLDAVTAAGFGVIQLDYASPGLDSMPS
                     AIPADVQATIRDETVARGIEIAGLHGTYNMIHPDLEVRAAGIASLRALAASCAATGTS
                     IIALSTGTRDATNMWRRHPENDSPEAWDALIEELTKALAIADEFGVTLAFEPEPANVA
                     KSAVKARELLDQVHHPRLKICFDAANIAASDLTRDPRLVIDDAFELLGDDIVLAHGKD
                     ITASGEFCPAGTGIVPWAWCIERLGEIGYDGALILHSLKEADIPRAVAAMTPGT"
BASE COUNT          675 a         1125 c         1218 g          746 t
ORIGIN      
        1 gaggatgatc gcggggaccg tcgcaagccc cgcgtcggat gccgcgttga tccggcgatt
       61 gccatcgatc acgcggaggg cgaggacgcc gtcctcatcc ggggtacgcg tgacgaggat
      121 cggaaccagc acgccgtgcc gcttgacgtg ctcgaccatg cgctccgtgg gacggggacc
      181 ccaggtctgg ccgaggtctt cgatgggaat ctcttcgatc tgataggtcg attccatggc
      241 aatgctccgg tcgggtcgag ggacgaggat gaactacggg aattgtcgcg gcgtttgttg
      301 ctggcaacaa gtacgtttgt tctaaatcaa tgtgagcgga tatcccggat agggcaatgc
      361 ccgatacaat agacgaagag atgcgtgtag gaggaggcga gcaccatgac tgaatcgggc
      421 gtgaagacgg agttgcctcc gatcatcgcg cggcatctgc cggagatcgt cgcgctcgcg
      481 aaatcgtacg gcgtcgaacg gcttgaggtc ttcggctccg tgatgaccga tcggtacgac
      541 cctctgttga gcgatgtcga tttcctcgtg aagtatccga gaggttacga ttacggtccc
      601 tttctcaagc actatcagag atttgaatca gatctcgcag aactcctgca ctcccccgtc
      661 gatctggtga tggatagcca gtccctgcga gatcgattcc gtcggaacat cgccgggact
      721 cgaaccgtgc tatacgatgc tcgataagac ccgggatcga cttgaggacg ttctcgctgc
      781 cgcggcgtac attgcctctg ttgccgaatc aatgagcgag gcggagtacg ttgcgaacga
      841 catggcgcga ttcgcgatcg agcgaaatct catcacgatt ggcgaagctt tggcacggat
      901 ccgaagcact gaacgcagca tcgcagatct catctcgact tccgaagagg tcgtagggat
      961 gaggaattcc atggttcacg aatacaagaa gacggacgat gtcgcggtat ggcagaccgt
     1021 aacggtgttt cttccggcac tggtcgagga agtggaggcg atccttgatg cgcaatcatg
     1081 aggagagatc gatgcggaac ccgtttcttt ccgttgcggc ggcccaggtc gcgcctgatt
     1141 tcggcaatgt cgaggcgaat gtccagatcg cgactgactt cctgcgggag gctgccgggc
     1201 aggagatcga cctgctcgtc ttccccgagc tctcgctgat cggctacgac ctcgggcagc
     1261 tcgatcgacc cgaagtctgg atcactgccg acgacgagcg gctcgccccg ctgcgccagg
     1321 tggtggtcga gaccgggatc atgacgatcg ttggcggtcc gtatcgcgat gaagacggcg
     1381 tgccgcgcct ctcctcgttc gtcctccacc cggacggatc gcgggagatc gtcttcaaga
     1441 tgcacattcc cggtgacgag cagtcccact tcatcgcggg tccctacccg ccgaagatcc
     1501 ttgagctcaa tggctggaac gtggcggtcg cgatctgctt cgatgccgcg aacccgcagc
     1561 acgcgatggc ggcgcgggcg gagggggccg atgtctacgt cgactccgcg ctctacgtga
     1621 cgagcgagat tcgccggatg gatctccatt tcgcttcccg cgcgatggat caccggatgt
     1681 tctcgctgct ggccaatcat gcggagacgg gtccaggctg ggaatcgtgc ggtcgcagcg
     1741 ggacgtgggg accggacgga gaggtccgcg tcacctcggg cgtcgagcca cgactcgtga
     1801 tcgaccgcct gccctatgcc gacctcgagc gataccgcct gcgtcccgtt cacgccggga
     1861 catcggccga ctgatctggc gcaaggaggg gcggctccta tgtcgcccct cgcgttgaca
     1921 acgtcctccc gagctcgtcg agggatcagc cggcgtccac gggcttccca gccgacacgc
     1981 ttcccggagt ggggtcgaca ggcgaggcat cgtgcatggg atgtggtccc tcgactgcgc
     2041 tccgtcgcgc gcctgacggc tgcgcgtcgt cgctccgctc gggatgacgg aatggaaagg
     2101 cgtcgagcgt gatgggctgc gctgccccgg tcagcggacc ttgcatgcgg tgtccgccag
     2161 gacacgtcct cccgagttcg tcgcggaatt gcatggcatt caaggcgcgt acccgggcag
     2221 cggcgcagca aacacgtctt cccgagcctg tcgagggatc actcggcatg caaggggctt
     2281 ggcggtcaag tttcccgcca gtcctcaccc aaatccagcc aatcgggatt ggctgcttcg
     2341 atgagagcga tcttcttctc ccgcctccat ccctttactt gtttctcgcg cgcgatagca
     2401 tctcgcgggc tcgcaaaatc ctcgaagtag acgagttggt cgcaaccatg ctcagaagca
     2461 aagccggtcg tgatccctcc tcgatgctcc cataggcgac gttcaagact gttcgtgacc
     2521 ccgatgtaaa tcgtgcgttt cgaattcgcc aggatgtaaa cgaagaagtc ggacactgct
     2581 atctccttga gccaggcgag atgcatcgcg ctctattctc gggcaccatg gtggcatctg
     2641 gagtggcggg caacgctagt gccacgtgat ccctcgacaa gctgctctga aaaactccgc
     2701 ccttcgggct ctccgaccca agcttttcat gccgcgctgg cgaggcgagc gcttcatgac
     2761 tactcgggag gacgggttcc gggaggcgtt gcgccggtat gcgccttgca tgcggagtga
     2821 tccttcgaca aggtcgggag gacgtatcgg ggcggggagc ccggaaaatc tatgtgcctg
     2881 gtgtcatcgc ggcgacggcg cgcgggatgt cggcttcctt gagcgagtgg aggatcaacg
     2941 cgccgtcgta gccgatctcc cccaggcgct cgatgcacca cgcccacggc acgatcccgg
     3001 tgcccgccgg gcagaattcg ccgctggccg tgatgtcctt gccgtgtgca aggacgatgt
     3061 cgtctcccag gagttcgaac gcgtcgtcga tgacgagcct cggatcgcgg gtcaggtccg
     3121 aggccgcgat attcgccgcg tcgaagcaga tcttcagacg tggatgatgc acctggtcga
     3181 gcagttccct cgctttgacc gcgctcttcg cgacgttcgc cggctcgggc tcgaaggcga
     3241 gcgtcacgcc gaattcgtcc gcgattgcga gtgccttcgt caactcctcg atcaacgcat
     3301 cccacgcttc tggcgagtcg ttctccggat ggcggcgcca catattcgtg gcatcgcgcg
     3361 ttccggtcga cagcgcgatg atcgacgtcc cggtcgcggc gcaggacgcg gccagggcac
     3421 gcagactcgc gattccggcg gcgcgaacct cgaggtccgg gtgaatcatg ttgtacgttc
     3481 catgaaggcc cgcgatttcg atcccgcgtg cgacggtttc atcgcggatc gtggcttgaa
     3541 cgtcggctgg gatggcagat ggcatcgagt cgagtccggg tgaggcgtaa tcgagctgga
     3601 tgacgccgaa tcccgccgcg gtcacggcgt cgagcgtcgc ggcgaatgtg ggtcgggcga
     3661 ggacggtttc gaagattccg agtttcatct ggtgactcca ggctggatcg gcggaagata
     3721 ctcgctattg aacaggcgat cgctccggtt ggccagaggt aacc
//
LOCUS       DASTMD010000437         3760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1649226, whole genome shotgun sequence.
ACCESSION   DASTMD010000437 DASTMD010000000
VERSION     DASTMD010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649226"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFQ54_06330"
     CDS             complement(<1..571)
                     /locus_tag="VFQ54_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU0164646.1"
                     /translation="MSESVMTEMKGRARFLAATHREAVDRTPVWFMRQAGRSLPEYRA
                     LRQRHDFMTVAHTPELAVEVTLMPVDRLGVDGAVLFADIMLPLEGMGVEFEIRAGVGP
                     VIAHPIRTAEDVARLRVVDPEEGTPYVLEAFKLLRRELGDRAAALGFAGAPFTLACYM
                     IDGRSTKEYPLTKALMFGEPDIWHALMEKL"
     gene            complement(603..1601)
                     /gene="hemB"
                     /locus_tag="VFQ54_06335"
     CDS             complement(603..1601)
                     /gene="hemB"
                     /locus_tag="VFQ54_06335"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU0164647.1"
                     /translation="MSLASTDISTLPGFIRTRRLRRSPQIRDFVRENELDPRDFVYPL
                     FVAEGEGIRNEISSMPDQYQLSLDHLEAEIDELKSLGIPAVLLFGIPAHKDAIGSGAF
                     DTDGIVQSATRLIKAKAPEMLVITDVCACEYTDHGHCGILVDGVVDNDQTLPLLARTA
                     LSHAAAGADIVAPSDMMDGRVAVLRQALDGAGFNEIPVMSYAAKYASGYYGPFREAAE
                     STPAFGDRRSHQMDPGNAREAMREIAIDLDEGADLIIIKPALAYLDIIRAARDRFDVP
                     LTAYNVSGEFAMIKAAARLGWIDEERVTLETLLSMKRAGASRIITYHAKEVSRWLA"
     gene            complement(1598..3340)
                     /gene="hemC"
                     /locus_tag="VFQ54_06340"
     CDS             complement(1598..3340)
                     /gene="hemC"
                     /locus_tag="VFQ54_06340"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEU0164648.1"
                     /translation="MSNFVAMPTGTRPLLIGTRGSALALWQAHAVRDRLQDGAGVSAA
                     ELKVINSDGDRDKQSPLAAIGGRGVFASALQHALLAGEIDAAVHSAKDLPGLTPEGLE
                     IAAFPERENPLDAIVSRHGVGLSELPPSPVIGTSSRRRAVQILALRPDATIVELRGNI
                     DTRLRKSRSGDYDAIVIAAAGLIRMGWEDQITEVLAIDQFTPSPGQGVLAVETRLAPD
                     AAASAVRRLDDEVVRLAIEAERAFLQGVGGGCTSPIGAFARLDGDRLEFFGMLASEDG
                     DRIERVHGWFPLATARDRIFDAATRMLASIEGSWPGAPLASGQVASLRELRVLITGTP
                     AVADGQAAQLELEGAIPVYVPTVRIEPAGDQVAIDRAAGSLVDGDVDWLVVTSANAVA
                     PIAERLGGRREIAPSVQVAVVGERTAEALSNAGIRVDIVAGGGGATGLLAALEAQEMV
                     GQRVLCLLSDIARSELVDGVRARGADVEVVIAYRTIAVDELDPGLRESLKAEPVDGVF
                     FSSPSAVASLRTLLGADANVLDRAHLVAIGETTAEAMREVGLTVGAIASSPDPAAMIA
                     ALRSLLPYDERTSA"
BASE COUNT          674 a         1266 c         1230 g          590 t
ORIGIN      
        1 tcagcttctc catcaaggcg tgccagatgt cgggctcacc gaacatcagc gccttggtga
       61 gcggatactc cttcgtcgag cgcccgtcaa tcatatagca ggccagggtg aacggagcgc
      121 cggcgaatcc gagcgccgcc gcccggtcgc cgagttcccg ccggagcagc ttgaacgcct
      181 cgaggacgta tggcgtccct tcctctggat cgacgacgcg aagacgcgcg acgtcctccg
      241 ccgtccggat cggatgcgcg atcacgggac cgacgccggc ccggatctcg aattcgacac
      301 ccatcccttc gagcggcagc atgatgtcgg cgaagaggac cgcgccatcc acaccgagac
      361 ggtcgaccgg catcagcgtg acttcaacgg cgagctccgg cgtgtgggca acggtcatga
      421 agtcatgacg ctggcggagg gcgcggtatt ccggcaggct tcgcccggcc tggcgcatga
      481 accagaccgg cgtccgatcg acggcttcgc gatgcgtggc ggcgaggaaa cgggcgcggc
      541 ccttcatttc ggtcattacg gattcactca tatctcgatg cagacctctt gcgtatgtcc
      601 agctatgcca gccagcggga gacttccttg gcgtgatagg taatgatgcg cgatgccccg
      661 gcacgcttca tcgaaagcag cgtctccagc gtcacgcgct cctcgtcgat ccagcccagt
      721 cgtgccgccg ccttgatcat cgcgaattcg ccggagacat tgtaggccgt cagcggaacg
      781 tcgaagcgat cccgcgccgc gcggatgatg tcgaggtagg cgagcgccgg cttgatgatg
      841 atgaggtccg cgccctcgtc gagatcgatc gcgatctctc gcatcgcctc gcgggcattg
      901 ccaggatcca tctggtggga acggcgatcg ccaaaggccg gtgtgctctc cgccgcctcg
      961 cggaacggcc cgtaatagcc agaggcgtac tttgcggcat acgacatcac cgggatctcg
     1021 ttgaatcccg cgccgtcgag cgcctgccgg agcaccgcga cgcgaccgtc catcatgtcg
     1081 gacggcgcga cgatatccgc gccggcagcg gcgtggctca gcgccgtacg cgccagcaac
     1141 ggcaatgtct ggtcgttgtc gacgacacca tcgacaagga tcccgcagtg gccgtgatcg
     1201 gtgtattcgc aggcacagac atccgtgatg acgagcatct ccggcgcctt cgccttgatc
     1261 aatcgcgtcg cgctctggac gatgccgtcc gtgtcgaaag ccccacttcc gatcgcgtcc
     1321 ttgtgcgccg ggatcccgaa gagcagcacc gccggaatcc cgagcgactt gagctcgtcg
     1381 atctccgcct cgaggtgatc gagggaaagc tggtactgat ccggcatcga ggagatctcg
     1441 ttccggatcc cctcgccctc cgccacgaag agcgggtaga cgaagtcgcg cggatcgagc
     1501 tcgttctcgc gcacgaaatc gcggatctgg ggcgagcggc gcagccgccg cgtgcggatg
     1561 aagccgggca gcgtcgaaat gtctgtggag gcgagactca cgcggaggtc ctttcatcgt
     1621 aaggaagaag cgaacgcagg gcggcgatca tggcggcggg atccggcgag gaagcgatcg
     1681 cgccgacggt cagtccaacc tcgcgcatcg cctcggccgt cgtctcgccg atcgcgacga
     1741 gatgcgcgcg atcgaggacg ttcgcatcag ctccgaggag cgtcctgagg ctcgcgacgg
     1801 cggacggcga cgagaagaag acgccatcga ccggctccgc cttcagtgat tcgcgcaggc
     1861 ccggatcgag ttcgtccacg gcaatcgttc gataggcaat gacgacttcg acatccgcgc
     1921 cacgggcgcg gaccccatcc acgagttcgg agcgggcgat gtcggagagg aggcaaagca
     1981 cgcgctggcc gaccatctcc tgggcttcca gcgcggcgag gagtccggtc gcgccaccgc
     2041 caccagcgac gatgtcaaca cgaatcccgg cattgctcag cgcttccgcc gttcgctcgc
     2101 cgacgaccgc gacctgcacg ctcggtgcga tctcgcgtcg tccgccgagc cgctccgcaa
     2161 tcggggcgac ggcattcgca ctggtcacga ccagccaatc gacgtcgcca tcgaccagcg
     2221 agccggcagc ccgatcgatc gccacctgat ccccggccgg ttcgatccgc accgtcggca
     2281 cgtacaccgg aatcgccccc tcaagttcga gctgagcggc ctggccatcg gcgacggcgg
     2341 gtgtccccgt gatgagaacc cgcaattcgc gcagggaggc gacctggccg gaagcgagcg
     2401 gcgctcccgg ccaggatcct tcgatcgacg ccagcatccg ggtcgcggca tcgaagatcc
     2461 gatcgcgggc ggtcgccaac gggaaccacc cgtgtacccg ctcgatgcga tcgccatcct
     2521 cggatgccag catcccgaag aattcgagcc tgtcgccgtc gaggcgagcg aaagcgccga
     2581 tcggcgaggt gcagccgccg ccgactccct ggagaaaggc ccgctctgct tcgattgcca
     2641 gccgcacgac ctcgtcatcc agccggcgga cggcggacgc cgccgcgtcc ggcgcaagcc
     2701 gcgtctccac cgcgaggacg ccctggccgg gggacggcgt gaactggtcg atcgcgagga
     2761 cctcggtgat ctgatcctcc caacccatgc gaatcagccc ggcggcggcg atcacgatcg
     2821 cgtcgtagtc ccccgagcga ctcttgcgaa gccgggtatc gatgttccca cggagctcga
     2881 cgatcgtcgc atccggtcgc agtgccagga tctgcacggc tcgccgccgg gacgaggtcc
     2941 cgatcacggg actgggcgga agctcactca agcccacgcc gtggcgggag acgatcgcgt
     3001 caagcggatt ctctcgctcc gggaaggcgg cgatttccaa tccttccggc gtcagaccgg
     3061 gaaggtcctt ggcggaatgc accgcggcgt cgatttcgcc ggccagcagg gcatgctgga
     3121 gggccgaggc gaagaccccc cgtccaccga tcgccgccag cggggactgc ttgtcccggt
     3181 ccccatccga gttgatgacc ttcaactccg ccgcggagac gccggcgcca tcctgcaacc
     3241 gatcgcgcac ggcatgtgcc tgccacagcg cgagcgcgct cccccgcgtt ccgatcagaa
     3301 gcgggcgagt tccggtgggc attgcaacga agttggacac gggaggacct gacactgatg
     3361 aaggaagatg cgcgaaatgc ggaaatccgt gaggcactcg cgccagaacg catggatgat
     3421 tctaggcagc gtccgaaagc gaatcaaggc agttctcccg attgcgcccg gctcgcggca
     3481 tgcctgcccg gacctcgccg cgggtgctgg ccgatccggt ctgcccaccg cgctgggagt
     3541 gcgatccccc ttgtccgcag atagacggac gccgtgcccg ggaagatcaa cgaagcttca
     3601 cgagagacat gaacctgaaa gcggcggcgg gatgtggaat ccggcgctcc gattgattgt
     3661 ccgctccggg aaatccgcat ccgggagcga gccgtcaagg accttggagg aaccggagac
     3721 gcggccccgt tcggctgtgg gtaggttcta gtggtagtcg
//
LOCUS       DASTMD010000438         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3076422, whole genome shotgun sequence.
ACCESSION   DASTMD010000438 DASTMD010000000
VERSION     DASTMD010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3076422"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            463..2268
                     /locus_tag="VFQ54_06345"
     CDS             463..2268
                     /locus_tag="VFQ54_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164649.1"
                     /translation="MTHYTAEDLVQDFRSGTLNRRQLWQRALAMGLTSTALASLLGTG
                     VIPATSVAAAVNAQSSGDLKLITASDEQASTWTKNFNPLLPQSSSNRWPTINGIYEPL
                     FVYNTIKAEIVPWLAESWSFDSTNTVLTFKIRQNVKWSDGTPFTAGDVAFTFNLFKQF
                     PALPGNGARTVIDNLATIEATDDATAVFTFGPAPVGEGTPAATPAGAAQPYTVGLYDI
                     GGQAIVAEHIWKDVKDPVTFTNETPVGTGPFTNVARFENQIWELHKNPSYWQEGKPYI
                     DGIRVPAYPNNDAINLATINGENDWAGNFIPDIEKVYTSKDSNNHYWFPATGATVFLY
                     LNTTVAPFDDVNVRKAISMAINRDQIVAIAEYDYTHPADATGMSDAFDTWKVKDIASA
                     DWVSYNKDKANQMLDDAGLKKDGDVRKTADGKALEYELNVVSGWSDWVQSVDIISKNL
                     ADVGIKATVKPYEQATWQTNVQNGDFTMSIGWSSQGATPFNFYRGVMSTNTWRPIKTS
                     AGENWQRFKSTDADALLDKFAATSDTAEQKDLANQLQKVFSDNAPAIPLFPGPQWGEF
                     NTKRFTGFPSADDPYTLLSTYANERLILLTTIKPV"
BASE COUNT          481 a          823 c          682 g          381 t
ORIGIN      
        1 tgcgccagcc ggcgaagatg atcggccgcc gtgccgtgga gctcctcgtc cagatgctga
       61 accagcccgt catcgagccc atccaggaat ggattccgac cgaactcatc ctgcgccggt
      121 cgtgcggttg tccgcatccc gccgccagtt cctccccaac gaatggccac cacacgaccg
      181 gcgacgatgg cggcgacccc gcatccggcg ttcgcctcgc ggcgcctcgt tcccgttccc
      241 gttccgcggt tggcagaagt gccaccaccc cgaatcgtca attcccacgc cttgcggtcg
      301 atgttccgca gcggaaacca acagttcagg ccttcgggtc gccagaaagc gaggtgagaa
      361 gagcgccgcg ggccagggtc cgcccgatct ggaatcagga aacgtcattt tcgcgatgca
      421 catcaccaac tcggccgctc cccggccgtt gggaggtcaa cgatgactca ttacaccgct
      481 gaagatctgg tacaggactt ccggtcaggc acattgaacc gccgtcagct ctggcagcgc
      541 gcgctggcga tgggactcac ctccaccgcg ctcgcctcgc tgctcggcac cggcgtgatc
      601 ccggcgacct ccgtcgccgc tgcggtcaat gcccagagtt cgggcgatct caagctcatc
      661 accgcgtccg acgagcaggc ctcgacctgg accaagaact tcaacccgct cctcccgcag
      721 tcgtcgagca atcgctggcc gacgatcaac ggcatctacg agccgctctt cgtgtacaac
      781 acgatcaagg ccgaaatcgt gccgtggctc gccgagtcgt ggtctttcga cagcacgaac
      841 accgtgctca cgttcaagat ccggcagaac gtgaagtggt cggatggcac gccgttcacc
      901 gcgggtgatg tcgccttcac cttcaatctc ttcaagcagt tcccggcgct cccgggcaac
      961 ggcgcccgca ccgtgatcga caacctcgcc acgatcgagg cgacggatga cgcgaccgcc
     1021 gtcttcacct tcggcccggc gcccgtcggc gagggcaccc cggcggcaac gcctgccgga
     1081 gcggcgcagc cctacacggt cgggctctac gatatcgggg gccaggcgat cgtcgccgag
     1141 cacatctgga aggatgtgaa ggacccggtc accttcacga acgaaacccc ggtcggtacc
     1201 ggcccgttca cgaacgttgc ccgcttcgaa aaccagatct gggagcttca caagaacccg
     1261 tcgtactggc aggagggcaa gccctacatc gacggtatcc gcgttcccgc ctacccgaac
     1321 aacgacgcga tcaacctcgc gacaatcaac ggcgagaacg actgggccgg caacttcatc
     1381 ccggacatcg agaaggtcta caccagcaag gattcgaaca atcattactg gttcccggcc
     1441 accggcgcca ccgtcttcct gtacctcaac acgacggtcg ccccgttcga cgacgtcaac
     1501 gtccgcaagg cgatctcgat ggcgatcaac cgcgaccaga tcgtcgccat cgccgaatat
     1561 gactacacgc atcccgcgga cgcgaccggc atgagcgacg ccttcgatac ctggaaggtg
     1621 aaggacatcg cgagcgccga ctgggtctcc tacaacaagg acaaggccaa ccagatgctg
     1681 gacgacgccg gcctcaagaa ggacggcgat gtccgcaaga ccgcggacgg caaggcgctc
     1741 gagtacgagc tgaacgtcgt ctccggctgg tccgactggg tgcagtcggt cgacatcatc
     1801 tccaagaacc tcgccgatgt cgggatcaag gcgaccgtca agccatacga gcaggcgacc
     1861 tggcagacca acgtccagaa cggcgacttc acgatgtcga tcggctggag ttcccagggt
     1921 gcgaccccgt tcaacttcta ccgtggcgtg atgtcgacca acacctggcg cccgatcaag
     1981 acgtccgccg gggagaactg gcagcgcttc aagtcgacgg atgccgatgc actcctcgac
     2041 aagttcgcgg caacgtcgga caccgccgag cagaaggacc tggcgaacca gctccagaag
     2101 gtcttctccg acaacgcccc ggcgatccca ctcttcccgg gcccgcagtg gggtgaattc
     2161 aacaccaaac gcttcacggg attcccgagc gcggacgatc cgtatacgct gctctcgacc
     2221 tacgccaacg agcgattgat cctcctcacg acgatcaagc cggtctaggt gagcatgagc
     2281 gggaggtggg gtgtcccatc tcccgccctt cgcgcacttc ctttggggag gaagttggcg
     2341 ctccttgcga gaacagacca gcgatgc
//
LOCUS       DASTMD010000439         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2541872, whole genome shotgun sequence.
ACCESSION   DASTMD010000439 DASTMD010000000
VERSION     DASTMD010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541872"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2151
                     /locus_tag="VFQ54_06350"
     CDS             <1..2151
                     /locus_tag="VFQ54_06350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU0164650.1"
                     /translation="ICPIETPEGPNIGLIGYLASYGKINPYGFIETPYRRVLSTIDVH
                     NQDIPLVGQILRDDVVNTTTGEIVATQGTEIDATIENAIKDAGVQFVRIRPVASTIID
                     YLPADREQNVMIAQADTPLDEAGRIKVDSVTVRLGSDGHSFPQVPPEQVHYMDVSPKQ
                     VVSVATALIPFLEHDDAQRALMGANMQRQAVPLVRPQAPVVGTGVEHITARDSGQVVV
                     SHHEGTVVSASGKQIVIVDDEGEKHVYHLQKFVRSNQDTCINQRPSVHIGERVTKNQI
                     IGDSSSTDKGELALGQNVLVAFMPWEGGNFEDAILLSERLVRDDVYTSIHIEKYECEA
                     RDTKLGPEEITRDIPNVGEESLANLDETGIIRIGAEVRPNDILVGKVTPRGETELSAE
                     ERLLRAIFGEKAREVKDTSLRVPHGVHGKIVDVKQFRRDDTSDHELPAGVNQSVRVMI
                     AQKRKVSEGDKMAGRNGNKGVISRILPIEDMPYLPDGTSVDIILNPIGVPSRMNLGQV
                     METHLGWAAQKLGFTVATPVFDGAHESDIIDALRAAGLPEDGKVDLYDGRTGEKFDRP
                     VTVGIIYMLKLHHLVEDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYG
                     AAHILQEMLTVKSDDIVGRVKTYESIVKGEPIGEAGVPESFKVLVKELRSLGLSIDVI
                     NEDEQTVDFSEDNSRDLLSNLDRINLSGFERTAD"
BASE COUNT          465 a          737 c          757 g          406 t
ORIGIN      
        1 atctgcccga tcgagacgcc ggaaggaccg aacatcggtc tcatcggcta cctcgcgtcg
       61 tacggcaaga tcaatcccta cggcttcatc gagacgccgt accgtcgcgt gctttccacg
      121 atcgacgtcc ataaccagga catcccgctc gtcgggcaga tcctgcgcga tgacgtcgtg
      181 aacaccacga ccggcgagat cgtggcgacg cagggaaccg agatcgacgc gacgatcgag
      241 aatgcgatca aggatgccgg cgtgcagttc gtccgcatcc ggccggtcgc gtccacgatc
      301 atcgactacc tgccggccga tcgcgagcag aacgtgatga tcgcgcaggc ggacaccccg
      361 ctcgacgagg ccggccggat caaggttgac tcggtgacgg ttcgcctcgg ttcggacggg
      421 cacagcttcc cgcaggtccc gcccgagcag gtccattaca tggatgtctc gccgaagcag
      481 gtcgtttcgg tggcgacggc gctgatcccg ttcctcgagc atgacgatgc gcagcgcgcc
      541 ctgatgggtg cgaacatgca gcgtcaggcc gtaccgctgg tgcgcccgca ggctccggtc
      601 gttggtaccg gtgtcgagca catcacggcg cgtgactccg gtcaggtcgt ggtctcgcat
      661 cacgaaggca cggtggtctc ggcctccggc aagcagatcg tgatcgtgga cgatgagggt
      721 gagaagcatg tctatcacct ccagaagttc gtccgctcga atcaggacac ctgcatcaac
      781 cagcgcccga gcgtccacat cggcgagcgg gtgacgaaga accagatcat tggtgactcg
      841 tcctcgaccg acaagggcga gctcgcgctc ggccagaacg tgctcgtcgc gttcatgccg
      901 tgggagggtg gcaacttcga agacgccatc ctgctcagcg agcgtctcgt tcgcgatgat
      961 gtctacacct cgatccacat cgagaagtac gagtgcgaag cccgggatac caagctgggt
     1021 cccgaggaga tcacccgcga catcccgaac gtcggtgagg agagcctcgc gaatctcgac
     1081 gagactggaa tcatccggat cggcgccgag gttcgtccga acgacatcct cgtcggtaag
     1141 gtcacgccgc gaggcgagac cgagctgagc gcggaagagc gactccttcg cgcgatcttc
     1201 ggtgagaagg cgcgtgaggt gaaggacacc tcgctccgcg tgccgcacgg cgtccacggc
     1261 aagatcgtcg acgtcaagca gttccgccgc gacgacacgt cggaccatga attgccggcc
     1321 ggtgtgaacc agagcgtccg cgtgatgatc gcgcagaagc gcaaggtctc cgagggcgac
     1381 aagatggccg gtcgcaacgg caacaagggt gtgatcagtc gcatcctgcc gatcgaggat
     1441 atgccgtacc tgccggatgg cacgtcggtc gacatcatcc tcaacccgat cggtgtgccg
     1501 tcccgcatga acctgggtca ggtcatggag acgcacctcg gctgggcggc gcagaagctc
     1561 ggcttcacgg tcgcgacgcc ggtcttcgac ggcgcgcacg agtccgacat catcgacgcg
     1621 ctgcgcgcgg cggggctgcc ggaggatggc aaggtcgacc tgtacgacgg tcgcaccgga
     1681 gagaagttcg accggccggt gacggtgggc atcatctata tgctgaagct ccatcacctc
     1741 gtcgaggaca agatccacgc ccgttcgacc ggcccgtact cgctcgtcac gcagcagccg
     1801 ctgggtggta aggcgcagtt cggtggtcag cgattcggag aaatggaagt gtgggcgctc
     1861 gaggcctatg gcgcggcgca catcctccag gagatgctga cggtgaagtc cgacgatatc
     1921 gtcggccgcg tcaagaccta cgaatcgatc gtcaagggcg agccgatcgg cgaggccggc
     1981 gtgccggaat cgttcaaggt gctcgtcaag gagcttcgct ccctcggtct ctcgatcgac
     2041 gtgatcaacg aggacgagca gacggtcgac ttctccgagg acaactcgcg ggatctcctc
     2101 tcgaatctcg atcgcatcaa tctcagtggt ttcgaacgaa ccgccgacta atacacacac
     2161 aacccggatg gcgatggctg ccatcgccat ccggacgcat tcctctcagt ggcagttcgt
     2221 tctcatggag gaaccatgga agacaattat cgttttgatc cccttcctgc ccagaactcc
     2281 tcgctttcgc taagcgattc ccgggatccc taccgtcagg tggcgggcgc cggtggcaac
     2341 agcgggagtg gacgtgggac gcgtg
//
LOCUS       DASTMD010000440         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_754731, whole genome shotgun sequence.
ACCESSION   DASTMD010000440 DASTMD010000000
VERSION     DASTMD010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754731"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFQ54_06355"
     CDS             <1..417
                     /locus_tag="VFQ54_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164651.1"
                     /translation="DSLVNKESGLAGVSGISGDMRDVMAAADRGDAPAREAIDLFCYQ
                     ARKHIGALSAVLGGLDRLVFTGGIGEHAAAIRGGIAAGTEYLGVEVDHTRNDAHERVI
                     SPDGSPVEVMVIPTNEDLMIAQLVVSTIQAKPQSPR"
     gene            436..>2229
                     /locus_tag="VFQ54_06360"
     CDS             436..>2229
                     /locus_tag="VFQ54_06360"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEU0164652.1"
                     /translation="MSTLFHFDPAISSVVRSDVPVRAAVVPAEKPLSPDLLRRMHRYW
                     QAANYLTIGQIYLQENPLLREPLRKEHIKPRLLGHWGTSPGLNLIYTHVNRLINEHDV
                     DAIYLAGPGHGGPALVAQSYLEGSYSEIYPEITQDAAGMRRLFRQFSTPGGIPSHVSV
                     TTPGSIHEGGELGYVLAHAFGAAFDNPGLLVVAVVGDGEAETGPLAGSWKSTFFLDPV
                     HDGAVLPILHLNGYKIANPTTLGRSSNDDVRMSIEGQGYDVVFVEGDDPAIVHQAIAA
                     ALDSSYARIREIQEDARRNGVNGRPRWPAIVLRTPKGWTGPAVVDGVPIEGTFRAHQV
                     PLASVREEPDHLELLEAWMKGYDPDSLFDVDGRLQPEVASLAPKGVRRMGANPHANGG
                     PDLSALDLPDIHAYATEFPHPGTVNVGNTMPLGSWLRDVYAGNRDERNFRIFSPDELT
                     SNRLGAVLTDERRSFVGPLIDIDDALATDGRVMEVLSEHLCEGWLEGYTLTGRHGMFV
                     TYEAFAMVSASMTVQHSKWLQESRALPWRRPVPSLNILLTSTCWRNDHNGFSHQGPGL
                     IDVMLSKRGTVSRIYLPPDANCLLSITDHCLR"
BASE COUNT          399 a          713 c          706 g          411 t
ORIGIN      
        1 gactcgcttg tgaacaagga atccggtctt gccggtgtct ctgggatttc aggcgacatg
       61 cgagacgtga tggcagcggc cgaccgcggc gacgcgcccg cccgggaggc catcgatctc
      121 ttctgctacc aggcgcggaa acatatcggc gcgttgtcgg cagtgctcgg cggactcgac
      181 cggcttgtct tcaccggtgg gatcggggag cacgctgccg ccattcgtgg cgggatcgcc
      241 gccgggaccg aatatctcgg cgtcgaagtc gaccacaccc ggaacgacgc gcatgaacgg
      301 gtgatttcac ccgacggatc ccccgtggaa gtcatggtga ttccgaccaa cgaggatctc
      361 atgatcgctc aacttgtcgt ctccacgatt caggcgaagc cgcaatcccc ccgatgacag
      421 aaggagtatg cgcggatgtc aacgctcttc catttcgacc cggcaatttc cagcgtggtt
      481 cgatcggacg tgccggttcg tgccgccgtc gtgcccgccg aaaagccgct ttcgccggat
      541 ctgctgcggc gcatgcatcg ctattggcag gccgcaaact acctgacgat cggtcagatc
      601 tatctccagg agaatcccct gctgcgtgag cccttgcgga aggagcacat caagccgcga
      661 ttgctcggcc actggggaac gtcacccgga ctgaacctga tctacaccca cgtcaatcgc
      721 ctgatcaacg agcacgatgt agacgccatc tatctcgcgg gtccgggtca tggcggcccg
      781 gcgctggtcg cccagagcta cctcgagggt tcatacagtg agatctatcc cgagatcacg
      841 caggacgcgg cgggcatgcg gcgcctcttt cgccaattct cgactccggg cggaattccc
      901 agtcacgtca gcgtgacgac acccggctcg atccacgaag gcggcgagct tgggtacgtc
      961 cttgcgcatg cgtttggcgc ggcgttcgac aaccccggcc tgcttgtcgt ggccgtggtt
     1021 ggggatggcg aggccgagac cgggccgctc gccggctcct ggaaatcgac gttcttcctc
     1081 gatccggtcc acgacggcgc cgtccttccg atcctgcatc tgaacggata caagatcgcg
     1141 aacccgacga cgctgggacg ctcctcgaat gacgacgtcc ggatgtcgat cgagggccag
     1201 ggctatgacg tcgtcttcgt cgagggagac gatcccgcca tcgtccatca ggcgatcgcc
     1261 gccgccctcg actcctccta cgccaggatc agggagatcc aggaagatgc ccgccggaac
     1321 ggggtgaatg gtcgcccccg gtggccagcg atcgttctcc gcacgccaaa gggatggacc
     1381 ggtccggccg tggtcgatgg ggtgccgatc gaagggacct ttcgcgcgca tcaggttccc
     1441 ctggcatcgg tccgggagga gcccgatcat ctggaactcc tcgaagcatg gatgaaaggc
     1501 tacgatcccg actcgctttt cgatgtcgat ggtcggttgc aaccggaggt agcgtcgctt
     1561 gcgccgaagg gggtgcggcg gatgggggcg aatccgcatg ccaacggcgg tcccgatctg
     1621 tccgcgctcg atcttcccga cattcacgcc tacgccacgg aattccccca tccgggaacg
     1681 gtgaacgtcg ggaatacgat gccccttggc agctggcttc gcgacgtcta cgccggaaat
     1741 cgtgacgagc ggaatttcag gatcttcagc ccggacgaac tgacgtcgaa ccggctcggc
     1801 gcggtgctca cggacgagcg ccgctcgttc gtcgggccgc tgatcgatat cgacgacgcg
     1861 ctggcgaccg atggtcgggt gatggaagtc ctcagcgagc atctctgcga aggatggctc
     1921 gaaggctaca cgctcaccgg gcgtcacggg atgttcgtaa cgtacgaggc gttcgcgatg
     1981 gtctcggcgt cgatgacggt ccagcattcg aagtggctcc aggaatcgcg ggcgctgccg
     2041 tggcggcggc cggtaccgtc gctcaacatc ctgctgacct cgacctgttg gcgcaacgac
     2101 cacaacggat tcagccacca gggacctggc ctgatcgacg tcatgctctc gaagcgggga
     2161 acagtctccc ggatctacct cccgccggac gcgaattgcc tgctttcgat tacggaccat
     2221 tgcctgcgg
//
LOCUS       DASTMD010000441         6125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1113579, whole genome shotgun sequence.
ACCESSION   DASTMD010000441 DASTMD010000000
VERSION     DASTMD010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113579"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFQ54_06365"
     CDS             <1..408
                     /locus_tag="VFQ54_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164653.1"
                     /translation="TQSELWERPERQPEGANPLDPEKPIPHSLGDGGMWTTAEDLTRW
                     IKAMNADAFGVQELMKSKVSLNDGTPLDYAWAINVVEQNGVEICAHGGGWWGSISQMA
                     WVPAQGTGYIAFTVDGGEPLEKLNAALRERLTA"
     gene            complement(691..1452)
                     /gene="larB"
                     /locus_tag="VFQ54_06370"
     CDS             complement(691..1452)
                     /gene="larB"
                     /locus_tag="VFQ54_06370"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEU0164654.1"
                     /translation="MTATPRDPFTHLTAALALPDDPAAGDTIRLDPLRGERTGIPEVV
                     LAQHKTTPDVARSLIRLAEKQGRALASRLRDDQITDLATLVAAPSSIEIIAEARVAIV
                     AHDNASPPTSRGRIAVISAGASDRAVATEAAIMAREMGCEVAFVQDVGVAGLHRLVAP
                     LREIIDRGVDAIVVAAGMDGALPSVIAGLVAVPVIGLPVSVGYGYGGDGTGALMSMLQ
                     SCSPGIAVVNIDNGIGAGSMAALIANRAHDARRIS"
     gene            complement(1449..2282)
                     /locus_tag="VFQ54_06375"
     CDS             complement(1449..2282)
                     /locus_tag="VFQ54_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU0164655.1"
                     /translation="MAKSIRLDQALVERGLAETRSRAKSFIMAGDILVNGLKETRAGR
                     NVAEADELAFRSKPRFVSRGGEKLDHALTAFGIDVADEVAADLGASTGGFTDCLVQRG
                     ISRVYAVDVGYGQIDEKLRTDDRVIVMERENARYLESLPEPIDITVIDVSFISLALIF
                     PVVARISRPGARCVPLIKPQFEAGKGDVGKGGVVRDPAIHRRVIEHALASAIENGLMP
                     EGVVASPLRGPAGNVEFLANLALGVARPSDESLSAMIDQAMAEADRLVNDTPDVNEQP
                     E"
     gene            complement(2283..2789)
                     /gene="ruvC"
                     /locus_tag="VFQ54_06380"
     CDS             complement(2283..2789)
                     /gene="ruvC"
                     /locus_tag="VFQ54_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU0164656.1"
                     /translation="MSTNEPVTLGIDPGTAILGYGVIAGSRDPELVDVGVLETSNTTP
                     MPDRLVAIYEGLRELFARHRPDAIAIEQLFFARNVTTAIAVGQARGVALLAAAQARIP
                     VSEYSPSEVKHAVVGYGKADKRQIQEMVRLILKLKVAPQPDDAADALAVALCHVQTVP
                     FADRVRAS"
     gene            complement(2834..3580)
                     /locus_tag="VFQ54_06385"
     CDS             complement(2834..3580)
                     /locus_tag="VFQ54_06385"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0164657.1"
                     /translation="MSGHSKWSTIKRQKGATDAKRGQLFTKLAREITVAARTGVPDPE
                     ANASLRIAVQKARKENMPKDNIDRAIARATGSEGGAQYTESFYEGYGPGGTALMISTF
                     TDNKNRTVGELRAVLVRAGGTLGENGSVSWMFDQTGQITLPVNGADPEELALVAIDAG
                     ATDVQIDDDVIEIYTEPADLHKVNEQLVAGGYEPESVDLIMKPKDPLTPDVDVAVKAL
                     RLIEKLEDLDDVQTVFSNIEISDEVLAAVA"
     gene            4113..5285
                     /gene="rpoD"
                     /locus_tag="VFQ54_06390"
     CDS             4113..5285
                     /gene="rpoD"
                     /locus_tag="VFQ54_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU0164658.1"
                     /translation="MDSSLLTKGKDRGYLLSDEIIAAFPNAEEQMDSLEEFYSALVAE
                     GIEVFDQKPVAPVKKEAPVASTAKSRLREKLEAARGETEEPMPGVGVGDSVRLYLQEI
                     GETSLLTMEEEVWLSTRMERGKWCEEQLRDRVVSVEERAGLLADKADGDAARAHLIQA
                     NLRLVVSVAKKYIGRGLSFLDLIQEGNIGLMKATDKFDYHRGFKFSTYATWWIRQAIT
                     RAISDQSRTIRLPVHVGETINRVKKTSHRLQQILEREPTQEEIARAMDISDEKVRQVI
                     DVARHPVSLEAPVGSEGDAFLGDFIEDESMPAPLEVASQQLLRSQIGDALDKLTERER
                     RIIVLRYGLDDGRFRTLEEVGRDFGITRERIRQIEAKALRKLRHPSSSTQLRGYLD"
     gene            5440..6033
                     /locus_tag="VFQ54_06395"
     CDS             5440..6033
                     /locus_tag="VFQ54_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164659.1"
                     /translation="MLFKNMESDPPVGLTTPDFILRPIVAADAEMDYAAVMESREYLR
                     TWEQSSWPADDFTVAANRKDLEMLERRHGNREAFTYTVVDPAGTECVGCVYIMPPDAR
                     SLTKARITPVGDCRWEDYDAAVYFWVRRSRLAARTDRELLDALRTWLAQDWSLGRHLF
                     VTNEQFTQQVEMIERADLKVRFTIEDPDKPGRYLAYE"
BASE COUNT         1110 a         1912 c         2007 g         1096 t
ORIGIN      
        1 acgcagagcg aactctggga acgcccggaa cgccagcccg agggcgcgaa tccgctcgat
       61 cccgagaagc cgatccccca ttcgctcggc gatggcggga tgtggacgac ggccgaggac
      121 ctgacgcgct ggatcaaggc catgaatgcc gacgcgttcg gcgtgcagga actcatgaag
      181 tcgaaggtct ccctcaatga cgggacgccg ctcgactacg cctgggcgat caacgtcgtc
      241 gagcagaacg gtgtcgagat ctgcgcgcat ggtggcggct ggtggggaag catctcgcag
      301 atggcctggg tgccggcgca gggcacgggc tatattgcat tcaccgtgga cgggggcgag
      361 ccgcttgaga aactgaacgc ggctcttcgc gagcggctga ctgcgtagga gtggctggga
      421 cgcatttcga gacgtctgcc aggcgccgat agctccggaa ggtgggggga catagaagtc
      481 ccaccctacg gacgaccggc ttacgttcag cgtccgttcg tagggcggga cttctatgtc
      541 ccgcctttga ggtcacgcca tcgcgcccag gatttcgtgg atacaccact tacggggcga
      601 cttttatgtc gctccgtttg tcctggcgga ggacaccatg cggtgatgtt catatcgggc
      661 tgcgcccgat gggccacacg gggtggcccg ctacgaaatg cggcgggcgt cgtgggcccg
      721 gttggcgatg agggcggcca tcgatccggc gccgatgccg ttgtcgatgt tcacgaccgc
      781 gattcccggc gagcacgact gaagcatcga catcagggcg ccagtgccgt caccgccgta
      841 gccatagcca acgctgaccg gcaggccgat caccggcacc gcgaccagcc ccgcgatcac
      901 cgatggcagc gcgccgtcca tgcccgccgc gacgacaatc gcgtcgactc ccctgtcgat
      961 gatctcccgg agcggcgcca ccaggcgatg caacccggcg acaccgacgt cctgtacgaa
     1021 cgcgacctcg cagcccatct cgcgggccat gattgccgcc tccgtcgcca cggcgcggtc
     1081 cgatgcaccc gcgctgatga cggcgatccg cccgcggctg gtcggcgggc tggcgttgtc
     1141 gtgcgcaaca atcgcgactc tcgcctcggc gatgatctcg atcgaagacg gggccgcgac
     1201 gagcgtcgcc aggtcggtga tctgatcgtc acgcaggcga ctggccaacg ctctcccctg
     1261 tttctcggcc aggcgaatca atgagcgggc gacatccggg gttgtcttgt gctgggcgag
     1321 cacgacctcc gggatccctg tgcgctcgcc gcggagggga tccagacgga tcgtatcgcc
     1381 ggccgcgggg tcgtcgggca gggccagggc ggcagtcaga tgggtaaatg gatcgcgggg
     1441 tgttgctgtc actcgggttg ctcgttcacg tcgggagtgt cgttgacgag gcgatcggct
     1501 tccgccatcg cctggtcgat cattgcggaa agggattcgt cgctcggtcg ggcgacgccg
     1561 agtgcgagat tggccaggaa ttcgacattc ccggccggac cccgcagcgg cgatgcgacg
     1621 acgccctcgg gcatcagtcc attctcgatc gcgctggcca gcgcgtgttc gatcacgcga
     1681 cggtgaatcg cgggatcgcg gaccacgccg cccttcccga catcgccctt gccggcctcg
     1741 aactgcggtt tgatcaaagg cacgcaacgc gcgcccggtc tcgagatcct ggcgacgacc
     1801 ggaaagatca gcgcgaggga gatgaacgag acgtcgatca cggttatgtc gatcggctcc
     1861 ggcagtgact ccaggtagcg ggcgttttcg cgctccatca cgatcacgcg atcgtcggtg
     1921 cgcagcttct catcgatctg gccgtagccg acgtcgacgg cgtagacgcg agaaatgccg
     1981 cgctggacga ggcagtcggt gaagccgccc gtacttgcgc cgagatcggc cgccacctcg
     2041 tccgcgacat cgatcccgaa tgccgtcagt gcatgatcga gcttctcgcc gccgcgactg
     2101 acgaagcgag gcttcgaccg gaacgcgagt tcatcggctt cagccacgtt gcgaccggca
     2161 cgcgtttcct tgagtccgtt gacgaggata tcgccggcca tgatgaagga cttcgcccgg
     2221 gaacgcgtct cggcgagacc acgttcgacg agtgcctgat cgaggcggat cgatttcgcc
     2281 acctatgagg cccgcacgcg gtcggcaaag ggaacggtct ggacgtggca gagcgcgacg
     2341 gccagcgcgt ccgcggcatc gtcgggctgt ggcgcaacct tgagcttcag gatcaaacgc
     2401 accatctcct ggatctgacg cttgtccgcc ttgccatatc cgacaacggc gtgctttacc
     2461 tcggacggac tgtattccga caccgggatt cgcgcctgcg cggcggcgag aagggcgacg
     2521 ccgcgagcct ggccgacggc gatcgcggtc gtgacgttgc gggcaaagaa gagctgctcg
     2581 atcgcgatcg catcgggacg atgtcgagcg aacagttcac gcagtccctc gtagatggcg
     2641 acgaggcgat ccggcatcgg cgtggtattg gaggtctcca gaactccgac atcgacgagt
     2701 tcggggtcgc ggctgccggc gatcacgccg tagccgagaa tcgccgtgcc gggatcgatg
     2761 ccgagggtaa ccggttcgtt tgtggacacc ggagtctcct ttgacggcat cgattcggtc
     2821 agaaaacgag ctgctaggcg acggcggcga ggacctcgtc ggagatctcg atgttcgaga
     2881 agacggtctg cacgtcgtcg agatcctcga gcttctcgat caggcgcagc gccttgaccg
     2941 cgacatcgac atccggcgtg agcggatcct tcggcttcat gatcagatcg acggattccg
     3001 gctcgtagcc gccggcgacg agttgctcgt tcaccttgtg gaggtcggcg ggctctgtgt
     3061 atatctcgat cacgtcgtcg tcgatctgga cgtcggtcgc accggcatcg atcgcgacga
     3121 gtgcgagttc ctcgggatcc gcgccgttga ccggcagcgt gatctggccg gtctggtcga
     3181 acatccagct cacgctgccg ttctcgccga gggtcccgcc cgcgcggacg aggacggcgc
     3241 gaagctcgcc gacggtccgg ttcttgttat cggtgaaggt cgagatcatc agcgccgtgc
     3301 cgccggggcc gtagccttca tagaaggact cggtgtactg ggcaccgcct tcggatccgg
     3361 tcgcccgggc gatggcgcgg tcgatgttgt ccttcggcat gttttccttg cgtgccttct
     3421 ggactgcgat ccgtaacgag gcgttcgcct cggggtcggg cacgccggta cgcgccgcga
     3481 ccgtgatctc acgtgcaagt ttggtgaaga gctggccgcg cttggcgtcc gtcgcgccct
     3541 tctggcgttt gattgtggac catttggaat gtccagacat acctgacaaa ctcccgaatt
     3601 actggatcgt tccgcgtcga cccaaacccg agaattatag catccggtgc tgcgatctcc
     3661 gcaccgggcg gggattctcg gcgtggcgac ccgcaggatt tctggtcacg ataccatatg
     3721 ctcgtatagg gataatgata aatggcttat cgctactttg cgatatctcg tattttgtat
     3781 gcaaatgctt gaaatcggca aattggttcc gtagactgga gaaaagcaac agcaaggctc
     3841 acgggaactg aggaggacca tgggctgtat tcgagtcaaa actcagggtc cttgagttgg
     3901 tgaacgcccg acgcgtctcg cgtggggcgt tcgcatgcag tttcgtcgtt tgcagatcga
     3961 tcgatcgtcc ataacggcgc aaacacccgg accgggctgg aacccgggtg agccaccgcc
     4021 aaactggatt gatcggaact gccccgccgc gcaacgcccg tcgccgctga catcttcagc
     4081 gctgcgctcg cccgaaaggt ggaaacgctt ccatggattc gagcctgctt accaagggaa
     4141 aagatcgcgg atacctgctt tccgacgaaa tcatcgcagc ctttccgaat gcggaagagc
     4201 agatggattc gctcgaggag ttttattcgg ccctcgtggc cgaggggatt gaggtcttcg
     4261 atcagaagcc ggtggcaccg gtgaagaaag aggctcccgt ggcgtccacc gccaagagcc
     4321 gcctgcgtga gaagctcgag gctgcccgtg gcgaaaccga agaaccgatg cccggggttg
     4381 gggtcggcga ttccgttcga ctctatctgc aggaaattgg cgaaaccagc ctgctgacga
     4441 tggaagagga agtctggctc tccacccgca tggagcgcgg caagtggtgc gaggagcagc
     4501 ttcgcgatcg cgtcgtctcc gtcgaggagc gcgccggtct cctggccgac aaggccgacg
     4561 gggatgccgc tcgggcccac ctgatccagg caaatcttcg tctcgtcgtt tccgtcgcga
     4621 agaagtacat cggtcgtggc ctctcgttcc tggacctgat ccaggaaggc aacatcggcc
     4681 tgatgaaggc gaccgacaag ttcgattacc atcgcggctt caagttcagc acctacgcaa
     4741 catggtggat tcgccaggcg atcacccgcg cgatctccga ccagagccgc acgatccgcc
     4801 tccccgttca cgttggcgag acgatcaacc gcgtcaagaa gacgtcccac cgccttcagc
     4861 agatcctcga gcgtgagccg acgcaggagg agatcgcacg ggcgatggac atctccgatg
     4921 aaaaggtgcg tcaggtcatc gacgttgccc gccatccggt ctccctggag gctccggtcg
     4981 gcagcgaggg cgacgccttc ctcggcgact tcatcgaaga cgagtcgatg ccggcgcccc
     5041 tcgaggtcgc ctcgcagcag ctccttcgca gccagatcgg cgacgcgctg gacaagctga
     5101 ccgagcgcga gcgtcggatc atcgttctgc gctacggtct cgatgacggt cgcttccgca
     5161 ccctggaaga agtcgggcgc gacttcggca tcacccgcga gcgcatccgc cagatcgagg
     5221 cgaaggcgct gcgcaagctc cgccacccgt cgtcgagcac acagctccgc ggctacctcg
     5281 actagtcgtg cgtgtcccgc gaatggctat ccactcgcgg gacacaccat cgggactgga
     5341 tcgccgggtc cgtcgacgcg cggcgaaacc ggcaggtcga cgtcatcgcg atcgtcgata
     5401 tcggtgggtc tcgatgattg cccgacgcga aggagcggca tgcttttcaa gaacatggaa
     5461 tccgatcctc ctgtaggtct gacaaccccg gacttcatcc tccgcccgat cgtggccgcc
     5521 gatgccgaga tggactacgc ggcggtgatg gaaagccggg agtacctgcg gacgtgggag
     5581 cagtcgagct ggccggcgga tgacttcacc gtggcggcca accgcaagga cctcgagatg
     5641 ctcgagcggc gccatgggaa ccgcgaggct ttcacctaca cggtagtgga cccggccggg
     5701 acggagtgtg tcggctgcgt ctacatcatg ccgccggatg cgcgctcgtt gacgaaggca
     5761 cgcattaccc cggtcggtga ctgccggtgg gaggactacg acgcggcggt ctacttctgg
     5821 gttcgcaggt ctcgactggc agcccggacg gaccgtgaac tgctcgacgc actacgaaca
     5881 tggcttgcgc aggactggag cctgggcagg catctcttcg taaccaacga gcagttcacg
     5941 cagcaggtcg agatgatcga acgcgccgat ctcaaggtgc ggttcacgat cgaggacccc
     6001 gacaagccgg gccgctacct cgcctacgaa taggtccggc ggggagcagg tccgcatcgc
     6061 gccaacaggc aaccaaccgc gggactgacg ccccacaagg gtcccttccg cagacgcaca
     6121 tgagg
//
LOCUS       DASTMD010000442         6565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2184846, whole genome shotgun sequence.
ACCESSION   DASTMD010000442 DASTMD010000000
VERSION     DASTMD010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2184846"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VFQ54_06400"
     CDS             complement(<1..439)
                     /locus_tag="VFQ54_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164660.1"
                     /translation="MGWAVGKATTTHIEDPANTSDAGVERIQAERAIPAPLPLSRNDV
                     AQAERARGEQRNATIILLGIMGCVGLVVGGILFNAWDKRTTADEHLVDIVQWTIGIVL
                     TLALALVGYNWVSSNKAFERERDALDKLLDARDKWTDSELRRMD"
     gene            complement(750..1340)
                     /locus_tag="VFQ54_06405"
     CDS             complement(750..1340)
                     /locus_tag="VFQ54_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU0164661.1"
                     /translation="MEFTEVVRKRRMVRHFTPEPLPAETIERIVSLAQHAPSAGFSQG
                     VVFVAVTDEKLRKQVGTLSSEESYVTGGFDPFISEAPLQIVICTSEKVYHDRYNERDK
                     KPDGEDEIEWPIPYWHTDAGCSLMLVLLAGVDEGLGTAFVGAPDPKGLQALLSIPEDY
                     VPIGVAMFGYAAPDKKSPSLKRGRRSVDDVLHLNGW"
     gene            complement(1361..2845)
                     /locus_tag="VFQ54_06410"
     CDS             complement(1361..2845)
                     /locus_tag="VFQ54_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164662.1"
                     /translation="MISPPLRATITQHRRTRRNLRRGHPLVLGLGLLLQVVAGAVSWM
                     LVSHALTIAARGDADAAERRLYLIIFGALTGIVALTAIGMLGTALGKEQIALLLPMPL
                     DPATRWRMMLTQNLLTIVSPVAVVGTTIALARAGWSWAVVFLLWLPAAVAIGILLALT
                     LAWAFGGERLHVRQVAIGLGVWLGIDLVAFALLRNADFSRFPTPLLFIPDALLIATIS
                     FGPGAPILGRWYVGIARQMYATQSRSFRPLNPVIAWIGRRLAHRASPAVAVIVKEMRT
                     QTRDPFVLLRLAIVAAAIPLAALLDGRLAAFLGTSDPDRIVPVLVIALIAYGMVEITP
                     SPFGSEGNRLTIALLTQASPRSLIAGKATALVVPNLIEATAIFALLAWWHGISIGSTL
                     VAFVAIAIAVTGCCVLLTELSAWDSDLTRRVDDRSQAILVEHMPIGARRMLALGVTLA
                     AATGSALLIATQPCPLALPLLLAAHTITCILIGSRATRRLARSA"
     gene            complement(2842..4176)
                     /locus_tag="VFQ54_06415"
     CDS             complement(2842..4176)
                     /locus_tag="VFQ54_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0164663.1"
                     /translation="MTPISYSYTRDRSAALSAVLGWGILFALEASALSVLALIFLPTP
                     WLRIAAVLALIGPPGSIIIGMLAPFRTHHLLTSDALLLHYGRDRLTIPRGRIASVEAV
                     AQRQDTRYSPRFVHDPLVGRLSAVFSPHGQLLLHLDPPLDVPIRKGSFACREVLINVD
                     DRDLLLTELDVEPAAIPTIVVSPIAQQRPLPVASGETAIDTIALTRRYGSVAAVDNLE
                     LHIRRGEIYGLLGPNGAGKTTTITMLVGLLAPTSGAIRLAGHDLALDPLATRAAFGYV
                     PDRPILYDMLTGREFLEYVAQLRSIPTDVTQHRIARLIEMLELDRAADQLCRTYSFGM
                     KSKLALIAALLHEPHVLILDEPFNGLDPQSSQTIQQMLRDLAANGTAILISTHDLAIA
                     ERLCHRVGIIARGRLVAEGAPDEIRIGDDAPSLEETYLAIVGGRSAQQGAPR"
     gene            complement(4638..>6565)
                     /locus_tag="VFQ54_06420"
     CDS             complement(4638..>6565)
                     /locus_tag="VFQ54_06420"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEU0164664.1"
                     /translation="DTRSAPSATATGLVARAFTPLPLSQVRPMDWLQAQLRIQADGLH
                     GHLDEFWPDVARSRWIGGDKEGWERGPYWLDGMIPLAFLLDDDGLKAKSRHWVDAILA
                     GQQEDGWFGPLDNGIRQGYRYDPWPVFVVLKALIQYHEATDDERIPDAIARFLRRLDG
                     ILDEHPLRSWARYRWADLALSVFWLYERTDASWLLDLGAKIEAQGFPWRTHFERFPFR
                     DKCLSVECDLSSHGPNNAMGVKTAGVWYRQSHDPADRAATEQMIATLDRYHGQATGMF
                     SCDEHLGGTSPAQGTELCAVVEYQFSLEVLLAILGDAALADRLELVTFNALPATLTPN
                     MWARQYDQQVNQVVCRVVEADDKVYTSNGPDANIYGLEPHFGCCTPNHGQGWPKFAAN
                     LWMRSQDGGLAAVAWAPSAVRTEVAGTPVAIDVETDYPFTGEIGITVRTESPASFPLH
                     LRIPGWADGATLRIGDEESATVSAGAFHRVEREWAGETTIRLSLPMTVRVERRYNESA
                     AVFYGPNLLSLSPGEEWRQIGGEEPHADWEVYPTSAWNYALEIDPDDPAASISVTTGP
                     VGEMPFSPGGAPLSATVKGRRLPTWDLEHDAAAPVPSPPVVSDEPLEDLTLIPYGSTN
                     LRIAEFPILATAAATAR"
BASE COUNT         1188 a         2011 c         2281 g         1085 t
ORIGIN      
        1 cgtccatgcg acgtaattcg gaatcagtcc acttatctcg tgcatcgaga agtttatcca
       61 atgcatcgcg ctcccgctca aacgccttgt ttgacgagac ccagttgtag ccaacgaggg
      121 caagcgcgag ggtaagaacg atcccaatcg tccactgaac gatatcgaca aggtgctcat
      181 cagcggtggt tctcttgtcc cacgcgttga agagaatgcc acctaccacg agcccaacac
      241 agcccatgat tccgagaagg atgattgttg cattgcgctg ttcgcctcga gcacgctcgg
      301 cttgagccac atcattgcgg gacagtggta atggcgcggg tatggcccgt tcagcttgga
      361 ttcgctcgac cccagcatct gacgtgttgg caggatcttc tatatgcgta gtagttgctt
      421 tgcccaccgc ccaccccaac cgaagattcc tgccgtggaa agtttatgaa aacccaaaga
      481 ctatttacca ctttcagtgc aagtttggaa ggtgcagcac gaaaggagcc gtatccgaac
      541 tcgccgagcc ggatgacgga ttttgatgcg tcttgaaggt ctcgtcgcga gaagttgcca
      601 agggtctctc acgggagatc gctgtgataa tacggcccct tcgccgagat tcgcagtgca
      661 cgggcaaaga atccgccgta gaagcgaggg gcctgggaga acgatgaatt agttagggtg
      721 aaaaactggg cgatgcgtga gaatccctct taccagccgt tcaggtgcag cacgtcgtcg
      781 accgagcggc ggccgcgctt gagggacggt gatttcttgt ccggcgcggc atatccgaac
      841 atcgcgacgc cgatcggcac gtagtcctcg gggatgctga gcagcgcctg caggcccttc
      901 ggatccggcg cgccgacgaa tgccgtaccg aggccctcat ccacccccgc cagcaggacc
      961 agcatcagcg agcagccggc atcggtgtgc cagtacggga tcggccactc gatctcgtcc
     1021 tcgccgtccg gcttcttgtc ccgctcgttg tagcggtcgt gatagacctt ctcgctcgtg
     1081 cagatcacga tctggagcgg cgcctcggag atgaagggat cgaagccacc ggtgacgtag
     1141 gactcctccg acgagagggt gccgacctgc ttgcgcagct tctcgtcggt gacggcgacg
     1201 aagacgacgc cctggctgaa accggcactc ggggcgtgct gcgcgagcga gacgatccgc
     1261 tcgatcgtct ccgccggcag cggttcgggg gtgaagtggc ggaccatgcg tcgcttgcgg
     1321 acgacctcgg tgaattccat cggtgctcct tccagggcgt ctaggcggat cgggcgagcc
     1381 ggcgcgtcgc gcggcttcca atcaggatgc aggtgatggt atgcgccgcg agcaggagtg
     1441 gcaacgcgag cggacacggc tgggtcgcga tcaacagcgc cgacccggtt gccgccgcca
     1501 gcgtgacccc aagcgcgagc atccggcggg cgccgatcgg catgtgctcg accagaatcg
     1561 cctggctccg gtcgtcgacg cggcgcgtga gatcgctgtc ccaggcgctc agttcggtga
     1621 ggagcacgca gcagccagtc accgcgatcg cgatggccac gaaggcgacc agcgtcgatc
     1681 cgatcgaaat tccatgccac caggcgagca gggcgaagat cgccgttgcc tcgatcaggt
     1741 tgggcacaac gagcgcggtg gccttcccgg cgatcaggga gcgtggcgat gcctgggtca
     1801 gcagcgcgat cgtgaggcga ttgccttcgc tcccgaaggg gctcggtgtg atctcgacca
     1861 tcccgtaggc gatcagcgcg atgacgagta ccggaacgat ccgatccgga tcgctagtgc
     1921 cgaggaatgc cgcgagacgg ccgtcgagca gcgccgcgag cgggatcgcc gccgcgacga
     1981 tcgcaaggcg gaggaggacg aagggatcgc gcgtctgcgt gcgcatctcc ttgacgatca
     2041 ccgccacggc cggtgacgca cgatgggcca gcctcctccc gatccaggca atgaccggat
     2101 tcagcggccg gaacgaccgg gactgtgtgg cgtacatctg ccgcgcgatc ccgacatacc
     2161 agcgaccaag gatcggtgcg ccgggtccga acgagatcgt cgcgatcagg agggcatccg
     2221 ggatgaagag cagtggcgtc ggaaaccggg agaagtccgc gttgcgaagc agcgcaaagg
     2281 cgacgagatc gatcccaagc cagacgccga gcccgattgc gacctgacga acgtggagtc
     2341 gctcgccgcc gaatgcccag gcgagcgtca gcgcaaggag gatcccgatc gcgacggcgg
     2401 cggggagcca gagcaggaag acgaccgccc acgaccagcc ggctcgagcc aacgcgatcg
     2461 tcgtgccaac gacggcaacc ggcgagacga tcgtcagcaa gttctgcgtc agcatcattc
     2521 gccagcgagt cgcggggtcg agtggcatgg gaaggaggag agcgatctgc tccttgccga
     2581 gcgcagttcc gagcatcccg atcgcggtga gggcgacgat tcccgtcagt gccccgaaga
     2641 tgatgagata gaggcgacgc tcggcggcat cggcgtcacc ccgcgcggca atcgtcaacg
     2701 cgtgactgac gagcatccag gagacggcgc ccgctacgac ttgcaggagc agcccgaggc
     2761 cgaggacgag cggatgcccc cggcgcaggt ttcgtcgcgt acgccgatgc tgggtgatgg
     2821 tggcgcgaag cggcggcgag atcatcgcgg cgctccctgc tgtgccgacc gaccgccgac
     2881 gatcgcgaga taggtttctt cgagagacgg tgcgtcgtcg ccgatccgga tctcatccgg
     2941 cgcgccctcg gcgacgagac gaccgcgggc gatgatcccg acacgatggc agagccgttc
     3001 ggcgatcgcg aggtcgtgcg tggagatcag gatcgccgtg ccgtttgccg cgagatcgcg
     3061 gagcatctgc tggatcgtct gcgaactctg gggatcgagg ccgttgaacg gctcgtcgag
     3121 gatgagtacg tgtggctcgt ggagcagcgc cgcgatcagg gcaagcttgc tcttcattcc
     3181 gaacgagtag gtgcggcaga gctggtcggc ggcacgatcg agttcgagca tctcgatcag
     3241 gcgagcgatg cggtgttgcg tgacatcggt cgggatcgat cgaagctggg caacgtactc
     3301 gagaaactcg cgaccggtga gcatgtcgta gagaatcggt cgatcgggga cgtagccgaa
     3361 ggcggctcgc gtggcgaggg gatcgagggc aagatcgtgc ccggcgaggc ggatcgcacc
     3421 actcgtcggg gcaagaagtc cgacgagcat cgtgatcgtc gttgtcttgc cggcgccatt
     3481 gggaccgagc agcccgtaga tctcgccccg ccggatgtgg agttcgaggt tatcgacggc
     3541 ggcaaccgat ccgtagcgcc tggtgagcgc gattgtgtcg atcgcggtct ctccgctggc
     3601 gacgggtagc gggcgttgct gcgctatcgg gctcacgacg atcgtcggga tggcggctgg
     3661 ttcgacatcg agttctgtga ggagaagatc gcgatcgtcg acgttgatca ggacctcgcg
     3721 gcacgcgaac gaccccttcc tgatggggac atcaagcggc ggatcgaggt gaaggagcag
     3781 ctggccgtgc ggcgagaaga ccgcagagag gcgtccgacc aagggatcgt ggacgaagcg
     3841 cgggctgtat cgcgtgtcct gacgctgggc gacggcttcc accgatgcga tccggccgcg
     3901 cggaatggtg agccggtcgc gtccgtaatg gaggaggagc gcatcgctgg taaggagatg
     3961 gtgggtgcgg aatggggcga gcatcccgat gatgatcgac cccggcgggc cgatcagcgc
     4021 gagaacggcg gcgatcctga gccagggtgt ggggaggaag atcaatgcga gcacggagag
     4081 ggcgctcgcc tcgagcgcga agaggatgcc ccatccgagc accgcggaaa gtgcggcgct
     4141 gcggtccctg gtgtagctgt agctgatcgg cgtcattgcc ttgctccctg ctccagtcgc
     4201 gagatcttcg attgtgattg tggtgtctgg tcgtccctgg gacgtagccc caccctatca
     4261 ggagatcggg cccttgtgaa gataatcaca agggatccgt gtgcttcccg tatgccgccg
     4321 cacatgccgc gtcctcccga gcctgtcgag ggactgccgg aatccacgtg ctcccggcaa
     4381 gacccgtcgc cagccgtggt ggaacgggga gcatcatgca tgggacgtgg ttccgggcga
     4441 acaccaggtt cgcccctacg agatcgtgta cgtatgaccg tccccatcgt gagatctcac
     4501 aatggggatt gtcccgaacg gatcggctgc gccgacccga ggcggccacg agggccgcat
     4561 ctacggtgtt acggaagatc gcgatcggcc gggagccgcg agagcgccgc gattgcagcg
     4621 ctctcgcacg ctgcggctta tcgtgccgtc gcggcggcgg ttgcgaggat cgggaactcc
     4681 gcgatccgga ggttggtcga gccgtaggga atcagcgtca gatcctccag gggttcgtcg
     4741 gagactaccg gcggggacgg aaccggagcc gccgcgtcat gctcgagatc ccacgtcgga
     4801 aggcgccgtc ccttcaccgt cgccgaaagc ggcgcgccgc ccggggagaa tggcatctca
     4861 cccacgggac cggtcgtcac ggagatcgac gccgccggat cgtcgggatc gatctccagc
     4921 gcgtagttcc aggccgatgt cgggtagacc tcccagtcgg cgtggggttc ctcaccaccg
     4981 atctgacgcc actcctcgcc cggcgagagt gagaggaggt tcgggccgta gaagactgcc
     5041 gcgctctcgt tgtagcggcg ttcgacgcgg acggtcatcg gcaggctgag gcggatcgtc
     5101 gtctcgcccg cccattcccg ctccacgcgg tggaacgcgc cggcgctgac agtagccgat
     5161 tcctcatcgc cgatccggag cgtcgcgcca tccgcccacc ccgggatccg gagatggagc
     5221 gggaacgagg ccggactttc cgtccgcacc gtgatgccga tctcgccggt gaagggatag
     5281 tccgtctcga catcgattgc gacgggcgtg ccggcgactt cggtgcggac ggcgctgggc
     5341 gcccaggcga cggcggcgag accgccgtcc tgcgaacgca tccagagatt ggcggcgaac
     5401 ttcggccatc cctggccgtg atttggcgtg cagcagccga agtgcggctc cagcccgtag
     5461 atgttcgcgt ccggtccgtt gctggtgtag accttgtcgt ccgcctccac gacgcgacag
     5521 acgacctggt tcacctgctg gtcgtactgg cgtgcccaca tgttcggggt cagcgtcgcg
     5581 gggagggcgt tgaaggtgac gagctccagg cggtcggcga gcgccgcatc gccgagaatc
     5641 gcgagcagta cctcgaggct gaactggtac tcgacgacag cacagagctc cgtcccctgc
     5701 gcgggactgg tgccgccgag atgctcgtca caactgaaca tccccgtcgc ctgcccgtga
     5761 tagcggtcga gcgtcgcgat catctgctcg gtggcggcgc ggtcggcggg atcgtgcgac
     5821 tgccggtacc agacgcccgc ggtcttcacg cccatcgcgt tgttcgggcc gtgactggac
     5881 agatcgcact cgaccgagag gcacttgtcg cgaaaaggga agcgctcgaa gtgcgtgcgc
     5941 cacggaaatc cctgcgcctc gatcttcgcg ccgagatcga gcagccacga tgcgtccgtc
     6001 cgctcgtaga gccagaagac gctcagggcg agatccgccc agcggtagcg agcccacgac
     6061 cggagcggat gctcgtcgag gatcccgtcc agccggcgga ggaagcgggc gatcgcgtcc
     6121 gggatgcgct cgtcgtcggt cgcctcgtga tactggatga gcgccttgag cacgacgaag
     6181 accggccatg gatcgtagcg atagccctgg cggatgccat tgtcgagcgg accgaaccag
     6241 ccgtcctcct gctggccggc gaggatagca tcgacccagt gccgcgattt cgccttgagc
     6301 ccgtcgtcgt cgaggaggaa ggcgagcggg atcatgccat cgagccagta ggggccgcgt
     6361 tcccagccct ccttgtcgcc accgatccag cggctgcggg cgacatcggg ccagaactcg
     6421 tcgaggtggc cgtggagccc gtcggcctgg atccggagct gcgcctggag ccagtccatg
     6481 ggccgcacct gggaaagcgg gagcggcgtg aaggcgcgcg ccaccagtcc cgtggctgtg
     6541 gcggacggtg cggaccgggt atcga
//
LOCUS       DASTMD010000443         1707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1471715, whole genome shotgun sequence.
ACCESSION   DASTMD010000443 DASTMD010000000
VERSION     DASTMD010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471715"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFQ54_06425"
     CDS             <1..515
                     /locus_tag="VFQ54_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164665.1"
                     /translation="IVAVTDILLTLPTFLLAIFIVSLTSPSRVNIAIAILISTLPSIV
                     RIVRGDTLSIRNWDATLAARAIGARPSRIIFLHILPNITGSIAVVASLQIGNAILVEA
                     SLSFLGLGLSPPTPSWGLMINDGLRYLSTNPWMALVPGIAIMLTVMGFNLLGDGLRDA
                     LDPRTRSRGN"
     gene            539..1675
                     /gene="thiO"
                     /locus_tag="VFQ54_06430"
     CDS             539..1675
                     /gene="thiO"
                     /locus_tag="VFQ54_06430"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEU0164666.1"
                     /translation="MTTAQADMAVVGGGVIGCAIAWELSKEGHAVVLVERGELAREAS
                     WASAGIISPPRPNQRAELAMLGFILHNEIVPELEKRTGISTGSSRRGMIGLSLADDVA
                     PLREHSAWQQAQGIEAVWLDRDALREKEPAIHERFEGGLFNPGASSVLLSQVARMFAR
                     AAELEGARIVEHSQVFSIEMDGATATGLHTTKGFVPAGSVVVASGAWSSMFAENLDYP
                     IATIPVRGQIMAVAEPPIPLNAAVRHGGYYIYPRADGTIAIGATEEHESGFDARVTPQ
                     GLAELVAVIAELAPTLNQGRLSSYWAGLRPGTIDGESIIGKLPHLENVWIATGHFRSG
                     ALLATATARLLAQTIREGKQHPLLADFDPALRRTQLTAHTPATI"
BASE COUNT          307 a          554 c          540 g          306 t
ORIGIN      
        1 tcatcgtcgc ggtgacggac atcctgctca cgctgccgac attcctgctc gcgatcttca
       61 tcgtctcgct gacgtcgcca tcacgcgtca acatcgccat cgcgatcctg atctcgactc
      121 tcccgtcgat cgtccggatc gtccgcggcg acacgctgag catccgcaac tgggacgcga
      181 cgctggcggc aagagcgatc ggggcgcgac cgtcccgcat catctttctg cacatccttc
      241 ccaatatcac cggctcgatc gccgtcgtcg cgagcctcca gatcgggaat gcgattctcg
      301 tcgaggcaag cctgagcttt ctcggtcttg gcctctcgcc gccgacaccc tcgtggggac
      361 tgatgatcaa tgacggtttg cgatacctct cgaccaatcc gtggatggcg ctcgtcccgg
      421 gaatcgcgat catgttgacc gtaatgggat tcaatctgct tggcgacgga ctccgcgatg
      481 cgctcgatcc tcgcacgcgc tcgcgtggca attgacctgc aagtgagaag gaagaatcgt
      541 gacgacggcg caagctgaca tggcagtggt tggtggtgga gtgatcgggt gtgcgatcgc
      601 ctgggagctc tcgaaggagg gccacgcggt cgtgctcgtg gagcggggag agcttgcgcg
      661 cgaggcgagc tgggcctcgg ccgggatcat ctcaccgccg cgtccgaacc agcgcgcgga
      721 actcgcgatg cttggcttca tccttcacaa cgagatcgtg ccggaactgg agaagcgtac
      781 gggaatctcg acgggctcct cccgccgggg gatgatcggc ctcagtctcg cggacgacgt
      841 cgcaccgttg cgcgaacata gtgcctggca acaggcgcag ggtatcgagg cggtctggct
      901 cgatcgggac gcactgcggg agaaagagcc ggcgattcac gagcgattcg aaggaggact
      961 gttcaacccg ggcgcatcga gcgtgctgct ctcgcaggtc gcccggatgt ttgcccgggc
     1021 cgccgaactc gagggagcgc ggatcgtcga acattcgcag gtcttctcga tcgagatgga
     1081 cggcgcgacg gcgaccggat tgcacacgac gaagggcttc gttcccgccg gaagcgtcgt
     1141 cgttgcgagc ggcgcctgga gctcgatgtt cgccgagaat ctcgactacc cgatcgcgac
     1201 gatccccgtc cgcggccaga tcatggcggt cgccgagccc ccgatcccgt tgaatgccgc
     1261 cgtccgtcat ggcggctact acatctaccc ccgtgccgac ggcacgatcg cgatcggggc
     1321 gacggaggag cacgagtccg gattcgatgc acgtgtgacg ccacagggac tggccgagct
     1381 cgtggccgtg atcgcggaac tcgcaccgac cctgaaccag ggacggctct cctcctactg
     1441 ggcggggttg cgcccgggaa cgatcgatgg agaatcgatc atcggcaagt tgccgcacct
     1501 cgagaatgtc tggatcgcga ccggacactt ccgttctggc gcgcttctcg cgaccgccac
     1561 cgccagatta ctggcccaga ccatccgcga gggcaagcag catcccctgc ttgccgactt
     1621 cgatccggcg cttcgacgaa cacagctgac cgcacatact cccgccacga tctgacgaat
     1681 ttcgatcgac cgcacaaagg acagaca
//
LOCUS       DASTMD010000444         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2274444, whole genome shotgun sequence.
ACCESSION   DASTMD010000444 DASTMD010000000
VERSION     DASTMD010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2274444"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /locus_tag="VFQ54_06435"
     CDS             complement(<1..1806)
                     /locus_tag="VFQ54_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEU0164667.1"
                     /translation="MRPTSIQLPGSTPTTNGNNATRSPFFPEGISTFLLLLALVGCAS
                     MSVESGDWERIVIPVTFMGLSAAVFGSLLAKLRVLDSLAHFCSMLIGIFGSFGLVLTR
                     ADDLAPTLRGRVRPLLTVISDWYLGNGQVEGHEVYLVSILMGIIVWLVGYLAAWTLFR
                     RGWLMASVMLPGFLVLINLLYAPHADRRFLAAYLVIAIPLAARFHLYRRQREWSRARV
                     PSPASGGMKFLAIGAVLGILVTTAGWNAPPSLSQQAFQPLAQKMSSQISQLQDRIDTL
                     TQPTGAGQGSASAGSYASFGDAFTVGGALNLSNQPEMLVQGSQAPYLAAQRYDVYNGR
                     GWSSDVDSTFNQTTSDGKSYSPAMTFRSDQQVLLSGDVSEARSDSSVIVTPLTNRDSV
                     LFTIDTYLAANVPTSVRMSWRTVQDQPYKLDAQALGDLPPDVRGIATLLLQAKLTGQD
                     GGSGPLPVDSSSSSLITQEQANLEGRFLTVHWTAGTDGHVDTLYVSGQLPVYDDVETV
                     NGMGSQTEGEHYQVTGSASDATPDQLNQAGEDYPDWVVSRYLQQSSTVTTRTMELANE
                     IAPPGTTPYEKAEAIQNWLRTNIAYDETVSAPPPGD"
     gene            complement(1833..>2552)
                     /locus_tag="VFQ54_06440"
     CDS             complement(1833..>2552)
                     /locus_tag="VFQ54_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU0164668.1"
                     /translation="VLSRRDPATSATISGLREYTPGDPLNRISWVATARRGTMMVKEF
                     DPDPTSDLWIMIDLNESGVRKVAPPPGLDPEAYAPARSLDSTEEYLIALGGSLAERAL
                     EDGRRVGLVVNRSMPVRLMADNTRRQWYRIFESLAMARAFGNRPLGEAIAAESRRLSR
                     TAGLIVLTSSPDRSWVPAARSLVQRQVPMTVVVVDDGDVSGEQEKLDALIEDLASARV
                     RVTRYPVEYRDKVAVKSPDPV"
BASE COUNT          471 a          793 c          856 g          432 t
ORIGIN      
        1 gtcgccgggc ggcggcgcac tcaccgtctc gtcataggcg atgttcgtac gcagccagtt
       61 ctggatcgcc tcggccttct catatggcgt cgtgccgggt ggcgcgatct cgttcgccag
      121 ctccatcgtc cgggtcgtca ccgtcgacga ttgctgcaga tagcgcgaga cgacccaatc
      181 cggataatcc tccccggcct ggttgagctg atccggcgtc gcatcgctcg cgctgcccgt
      241 gacctgataa tgctctcctt cggtctgcga acccatcccg ttgaccgtct cgacatcgtc
      301 gtagaccggg agctgaccag agacatagag cgtatcgacg tgaccgtcgg tgcccgccgt
      361 ccagtggacc gtgaggaatc ttccctccag gttcgcctgc tcctgcgtga tcaggctgga
      421 cgaactgctg tcgaccggca gcggccccga tccgccgtcc tgtccggtga gtttcgcctg
      481 gagcagcagc gtcgctatcc cgcgaacatc cggcggcagg tcgccaagtg cctgcgcgtc
      541 gagcttgtac ggttgatcct gcaccgttcg ccaggacatc ctcaccgaag tggggacgtt
      601 ggccgcgagg taggtatcga tagtgaagag gacggagtcc cgattcgtca gcggtgtcac
      661 gatgacgctc gagtccgagc gcgcttcact gacgtcgccc gaaagcagga cctgttgatc
      721 tgaccggaag gtcatcgccg gcgaatagga tttgccatcg ctcgtcgtct ggttgaaggt
      781 ggagtcgacg tccgaggacc agccccggcc gttgtagacg tcatagcgct gtgcggccag
      841 atacggcgcc tgactaccct ggaccagcat ctcgggttga ttggagagat tcagggcgcc
      901 tccgaccgtg aaggcgtccc caaacgacgc gtaggagccc gccgaggcac tcccctgccc
      961 ggcccccgtc ggttgggtca gcgtatcgat ccggtcctgg agttgcgaga tctggctgct
     1021 catcttctgg gcgagtggtt ggaacgcctg ctgcgaaagc gatggcggtg cgttccagcc
     1081 ggccgtcgtc accaagatgc cgaggacggc gccgatcgcg aggaacttca tgccgccgga
     1141 ggccggggaa ggaacgcgcg cacgtgacca ctcgcgctgg cggcgataga ggtggaagcg
     1201 cgcggcgagg ggaatcgcga tcacgagata ggcggcgaga aaccggcgat cggcgtgggg
     1261 tgcatacagc aggttgatga ggacgaggaa gccagggagc atcaccgatg ccatcagcca
     1321 gccgcggcga aagagcgtcc atgccgcgag gtagccgacg agccagacga tgatgcccat
     1381 caggatcgag acgagataga cctcgtgccc ctcgacctga ccgttaccga gataccagtc
     1441 gctgatgacc gtcagcagcg ggcggacgcg accgcggagc gtgggagcga ggtcgtcggc
     1501 ccgcgtgagg acgagaccga acgagccaaa gatgccgatc agcatcgagc agaaatgggc
     1561 gagtgaatcg aggacgcgga gcttggcgag gagcgatccg aagaccgcgg cggagagtcc
     1621 catgaaggtg accgggatca cgatccgctc ccagtccccg gattcgacgc tcatcgaggc
     1681 acagccaacg agcgcgagga gaagaaggaa cgtcgagatc ccctcgggga agaatggcga
     1741 ccgtgtcgca ttgttgccgt ttgtcgtcgg tgtcgaacca ggtagttgga tggatgtagg
     1801 gcgcactgga gtcctcatac ctgcttttac gtctatacgg ggtccgggga tttgacggcc
     1861 accttgtcac gatactcgac aggatagcgg gtgacacgga cccgggcgct cgccaggtct
     1921 tcgatcaggg catccagctt ctcctgttca ccggaaacat caccgtcatc gacgacgacg
     1981 accgtcatcg gcacctgtcg ctggacgagc gacctggcgg ccggaaccca gcttcgatca
     2041 ggcgaggagg tcagcacgat caggcccgcg gtgcgcgaga gccggcgcga ttccgccgcg
     2101 atcgcctcgc ccagcggacg attgccgaag gcccgcgcca tcgccaggga ttcgaagatc
     2161 cggtaccact ggcgccgggt gttatccgcc atcagccgga ccggcatcga ccggttgacg
     2221 acgagaccga cgcgacggcc atcctcgagc gcgcgctcgg cgagcgatcc cccgagcgcg
     2281 atcaggtatt cctcggtcga gtcgaggctt cgcgcgggag cgtacgcctc ggggtcgaga
     2341 ccgggaggtg gcgctacctt gcgcacgccg gactcgttga gatcgatcat gatccagaga
     2401 tccgaggtcg gatcgggatc gaactccttc accatcatcg ttccccgccg cgcggttgcc
     2461 acccaggaga tgcggttcag cggatctccc ggcgtgtatt cccgcagacc ggagatggtt
     2521 gccgacgtcg cgggatcgcg gcgactcagg ac
//
LOCUS       DASTMD010000445         3249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1202431, whole genome shotgun sequence.
ACCESSION   DASTMD010000445 DASTMD010000000
VERSION     DASTMD010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202431"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..530)
                     /gene="rbsD"
                     /locus_tag="VFQ54_06445"
     CDS             complement(144..530)
                     /gene="rbsD"
                     /locus_tag="VFQ54_06445"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF008761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEU0164669.1"
                     /translation="MKRSGILHPGISHLLASTGHTAYFTVCDRGFPVPTEPERIDLAL
                     VDGIPTVLDVLRVVNAEFVIDRVIVAEEALGVSPEWIESLRSTLGDVPLQTVSHLELK
                     RLSHEGRATIRTGDTALYANIIIVSG"
     gene            complement(527..760)
                     /locus_tag="VFQ54_06450"
     CDS             complement(527..760)
                     /locus_tag="VFQ54_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164670.1"
                     /translation="MTGTTGRWEYHVEKIYGTIKVERLNELGGDRWELASTALGADNA
                     QVLIFKRPTPTLAEQITLDQRTRVQDEAGVEPS"
     gene            complement(757..1554)
                     /locus_tag="VFQ54_06455"
     CDS             complement(757..1554)
                     /locus_tag="VFQ54_06455"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0164671.1"
                     /translation="MTESPLRAGSRNRASAAPNTPASSLIQRRNLGSDVYHILWDRIL
                     DRELHPGEKLSDLRLSEELGVSRTPVREALHRLVQDGVVIAEPNRGFFVASYDRADIE
                     EIYALRAALESFALRNAVDTVSPDQFKWALAELERVEYLLRTAEKDDERTEATAAFLE
                     VDRGFHNLLLESSHNRRLIAILNGLWAQIAVFQKAGLMITGWIDNALDEHRIILNHLI
                     DGETDAAARALEHHIDSIRDRVLADFALNADDAGASENGVRAGKEDA"
     gene            1960..3135
                     /locus_tag="VFQ54_06460"
     CDS             1960..3135
                     /locus_tag="VFQ54_06460"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0164672.1"
                     /translation="MAVKLASRMGRLGTETAFEVLVRARALEAQGKDIVHLEIGEPDF
                     DTPENIIEAGANALKSGWTHYGPAAGDANLRTAIANYINGSRGTAFTADQVVVTPGGK
                     PVMFFLILALLEEGDEAIYPDPGFPIYQSMIDFSGAKAVPIPLREENDFRLDVDELAS
                     LVTDKTRLLIINSPANPTGGVLERSDIEAIAKIAVEHDLIVLSDEIYSELLYEGEHVS
                     IATFPGMADRTVILDGFSKTYAMTGWRLGYGLFPAELAPLISKLMVNSVSCTSVAVQQ
                     AGLEALTGPQDKVGEFREEFRKRRDLVVDRLNAIPGISCRKPKGAFYVFPNITGTGKS
                     SREFADLLLQEYGVAALSGTAFGAAGEGYLRLSTANSEENLNKALDRIAQAAKDVRA"
BASE COUNT          564 a         1018 c         1093 g          574 t
ORIGIN      
        1 gcgggattgc gtcccggcgt agagatcgag cgcccagccg ccggcgatgc accagatgcc
       61 cgggaagtcc cgaaagagca cgccggtttc cgccggggag agcggttccc acggacccag
      121 gtcgtcgttt gccatgatgt ctgttacccc gagacgatga tgatgttcgc gtagagcgcg
      181 gtgtcgccgg tgcggatcgt ggcgcgtccc tcgtgactga gtcgcttcag ctccaggtgg
      241 gaaaccgtct gcaacgggac gtcgccgagt gtacttcgca gcgattcgat ccactcggga
      301 ctgacgccga gtgcttcctc ggcgacgatc acgcggtcga tcacgaactc cgcgttgacc
      361 acccggagaa cgtcgaggac ggtcgggatg ccgtcgacga gcgcgagatc gatccgctcg
      421 ggctcggtcg gaacggggaa gccgcgatcg cagaccgtga agtaggccgt gtgaccggta
      481 ctcgccagga gatgactgat cccgggatgg aggatgccgg atcgcttcat gacggctcga
      541 ctcccgcttc gtcctggacc cgcgtgcgct gatcgagcgt gatctgctcg gccagggttg
      601 gcgtcgggcg cttgaagatc aggacctggg cgttgtcggc gccgagcgcg gtgctcgcca
      661 gctcccagcg gtcgccgccg agctcattca gccgctcgac cttgatcgtc ccgtagatct
      721 tctcgacgtg gtattcccag cggccggtcg ttcccgtcat gcgtcttctt tccctgctct
      781 gacaccgttt tcacttgcgc cggcatcatc ggcgttcaac gcgaagtcgg caaggacgcg
      841 atcgcggatg ctgtcgatgt ggtgctccag cgcgcgggcg gcggcatcgg tctcgccgtc
      901 gatcaggtga ttgaggatga tgcggtgctc gtcgagcgcg ttgtcgatcc agcccgtgat
      961 catgagaccc gccttctgga agacggcgat ctgcgcccac agcccgttga ggatcgcgat
     1021 caggcggcga ttgtgcgaac tctcgagcag gagattgtga aagccgcgat cgacctcgag
     1081 gaaggcggcc gtcgcctcgg ttcgctcgtc atccttctcg gccgtcctga gcaggtattc
     1141 gacgcgttcg agctcggcga gtgcccactt gaactggtct ggagaaacgg tatcgacggc
     1201 gttccgtagc gcgaacgatt cgagggcggc ccgcagcgca tagatctcct cgatgtcggc
     1261 gcgatcgtag gaggcgacga agaagccacg attcggctcg gcgatcacga cgccatcctg
     1321 cacgaggcgg tgaagcgcct cgcggaccgg cgtccggctg acacccagtt cctcgctcag
     1381 gcggaggtcg gacagcttct cgccgggatg gagttcgcga tcgagaatgc ggtcccagag
     1441 gatgtgatag acgtccgacc ccaggttccg gcgctggatc aggctcgagg ccggggtgtt
     1501 cggggctgcg gacgcacggt tccggctccc ggccctgagt ggcgattccg tcataaattc
     1561 gttcctcatc cttcgactga cgccggcatc tgacgctcca ctccgcttgc aagaccagga
     1621 tcgatatcat cgttgcgcgg tgatggtgcc ggtgaccgcc gcgtgtatca cggtgaacat
     1681 catcccgacc ggcagccgcc attcgacggg aaaatcccgc cggagcgcgt caacgacgtt
     1741 gtccagacat tgtatacaag atacgagagt cgtgtcagcg gatgatgtga gcctgccggg
     1801 ccggatcctg cctgccgccg gcgccctgtc tgtatgcatc caggggctcg tcggctcgct
     1861 caatcgcgcc cggcccgcga taatcgcacg agcagctgga aggcagggga tccgggtgcg
     1921 gcccggttcg gaagcggaca cgaacaagga gagaagtgca tggcggtcaa gctcgcgtcg
     1981 cggatgggac gattgggaac cgagacggcg ttcgaagtgc tcgtgcgagc gcgggcgctc
     2041 gaggcgcagg ggaaggacat cgtccacctc gagatcggcg agcccgattt cgatacgccg
     2101 gagaacatca tcgaggccgg cgccaatgcg ctcaagtccg gctggacgca ctacggtcct
     2161 gcggccggcg acgccaatct ccggacggcg atcgccaact acatcaacgg ctcgcgcgga
     2221 actgccttca cggccgatca ggtggtggtg acgccgggcg gcaagccggt gatgttcttc
     2281 ctcatcctcg cgttgctcga ggaaggcgac gaggcgatct accccgatcc cggcttcccg
     2341 atctaccagt cgatgatcga cttctccggt gcgaaggcgg tgccgattcc gctgcgtgag
     2401 gagaacgatt tccggctcga tgtcgatgaa ctggcgagtc tcgtcactga caagacgcgg
     2461 ctgctgatca tcaacagccc ggcgaatccg accggcggcg tgctcgagcg gtccgacatc
     2521 gaggcgatcg cgaagatcgc cgtcgagcac gacctgatcg tgctctcgga cgagatatac
     2581 agcgagctgc tctacgaagg cgagcatgtc tcgatcgcca cgttcccggg gatggccgat
     2641 cgcacggtga tcctcgacgg cttctcgaag acctacgcga tgacgggatg gaggctcggc
     2701 tacgggctct tcccggcgga actcgcgccg ctgatctcga agctgatggt gaacagcgtc
     2761 tcctgcacct cggttgccgt ccagcaggcc ggcctcgaag cgttgacggg accgcaggac
     2821 aaggtcggcg agttccgcga ggagttccgc aagcgtcgcg acctcgttgt cgatcgtctc
     2881 aacgcgattc cgggcatcag ctgcaggaag ccgaagggcg ccttctacgt cttcccgaac
     2941 atcacgggaa ccgggaagtc gagccgcgag ttcgccgatc tcctgttgca ggagtacggt
     3001 gtcgccgcgc tctccggcac cgcctttggc gcggcaggtg aggggtacct ccgcctctcg
     3061 acggcgaact ccgaggagaa cctgaacaag gcgctcgatc gcatcgccca ggcggcgaag
     3121 gacgtccgcg cgtagggcca gaacggcccg aagagcgatc cgagccgccg tagcgggtca
     3181 gctggtctga cccatccggc ggatccggat atgggcgccg gcccccgcga cgtcgaaggc
     3241 acggaaccg
//
LOCUS       DASTMD010000446         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1560030, whole genome shotgun sequence.
ACCESSION   DASTMD010000446 DASTMD010000000
VERSION     DASTMD010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560030"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1717
                     /locus_tag="VFQ54_06465"
     CDS             <1..1717
                     /locus_tag="VFQ54_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164673.1"
                     /translation="DNASAAQQLATQRMSDIEKILGDALSAAKIVIPAPAPFFLPASE
                     SAQHTWVQVPQGPDWVDFDPTLAGARSGDILATVATTMDQIPTDPYHVVTIRVVAEEV
                     VGGSPNRRDAATFTATSVEIGAQPITIGVTPPSGVAALGASISELGTGVVAYYPYITV
                     GGMNAAYATTPVSVTSGDQGLAGVLGDATPATGAAEGELLGLWYAVDITSPDREPISI
                     EREMYDRVGFANRGGATIDLTKVPPIQLATGTDGTSYAPGLSNVFAIGISSADLPSSY
                     ALASEDPFGGLSSLGSTHDAFWGSLLRGNLLSIGVQAFVDVPQVAAFTIGLADTSDPA
                     SDLLIRGDLVLNQRALIPASGGSAGSSLVHPALRTGILAQVAESVLLAPQFWSANGQV
                     TTGATVADVFEAAAKSGTKIAVLGDSSAPVPTALPGEANLRILTALKAGLIVVVPEEP
                     VSVGSSTWTGWWTIDPKTGATSDMMQNGMGYASIAVPGDPRVTFQDAAEEAEILTEVN
                     KWTPWYQRLGKCLAVVALLAAAGIDPSSLNPGSFTTSAGAAKVLYRVISRNPNVAKDI
                     ASCFG"
     gene            1751..>2415
                     /locus_tag="VFQ54_06470"
     CDS             1751..>2415
                     /locus_tag="VFQ54_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164674.1"
                     /translation="MLGFRCRLLRVVVLGLIAISGSVALGVTGADAVAAQGATPSGSS
                     NCVTVVEPNDQPPQATDLGTDVGCATSPNANGGQDVFKWTVDDAGAAKRWTISTSAIP
                     GQAVKLEIYTVTVDTNGVVTNPVKLTGVSGDSGQAASIENVLVAKGTYFIATAIGGGG
                     AYELTVSPGDSVPAANDTGDHASPETALPLQDAFALSGDRASGVDGFAWTLDDRAAAT
                     HWTL"
BASE COUNT          410 a          780 c          808 g          417 t
ORIGIN      
        1 ggacaacgcg agcgccgccc agcagctggc gacgcagcgg atgagcgaca tcgagaagat
       61 ccttggtgac gctctttccg ccgcgaagat cgtcattccg gcgccggcgc cattcttcct
      121 tcccgccagt gagtccgcgc aacacacctg ggtccaggtc ccgcaggggc cggactgggt
      181 ggatttcgat cccacgctcg ccggcgccag gtcgggagac atccttgcaa ctgtcgcgac
      241 gacgatggat cagatcccga ccgaccccta ccatgtcgtg acgatacgcg tcgttgccga
      301 ggaagttgtc ggcggtagcc ccaatcgtcg ggacgcggcg accttcaccg cgacgagcgt
      361 cgagattggc gcgcagccga tcacgatcgg cgtgacgccg ccgtcgggcg tcgccgcgct
      421 tggcgcctca atcagtgagc tgggcacggg tgtcgtcgcc tactacccgt atatcacggt
      481 gggaggaatg aacgccgcct atgcgacgac tcccgtcagc gtgacgtctg gtgaccaggg
      541 gctggccggg gtgttgggcg atgcgactcc cgcgacgggc gccgccgagg gcgaattgct
      601 tggcctctgg tatgccgtcg atatcacgtc gccggatcgc gaaccgatct cgatcgaacg
      661 cgagatgtac gatcgcgtcg ggttcgccaa tcgtggcggg gcgacgatcg atctgacgaa
      721 ggtgccaccg attcaactcg cgaccgggac cgatggaacc tcatacgcac ccgggctctc
      781 caacgtcttc gcgatcggaa tcagctccgc cgacctgcca tcctcgtacg cactcgcgtc
      841 ggaggatccc ttcggcggac tttcctcgct cggttcaacc catgacgcgt tctggggatc
      901 cctgctgcga ggcaacctgc tttcgatcgg tgtccaggcc tttgtcgacg tgccgcaggt
      961 tgcggccttc accatcggac tggcggacac ctccgatccg gcgagcgatc ttctgatccg
     1021 gggcgatctc gtcctgaacc agagagcgct gattcccgcg agcggcggat cggcgggatc
     1081 gtcgctcgtt catcccgcgc tccggaccgg catcctcgcc caggtcgcgg agagcgtcct
     1141 gcttgcgccg cagttctggt ccgccaacgg gcaggtgacc accggcgcga cggtcgcaga
     1201 tgtcttcgag gcagcggcaa agagcggaac gaagatcgcc gttctcggcg attcctcggc
     1261 cccggtcccg accgcgttgc ccggcgaggc aaatctgcgg atcctgaccg cactcaaggc
     1321 ggggctgatc gtcgtggtcc ccgaggagcc ggtctcggtc gggtcgtcga cctggacggg
     1381 ctggtggacg atcgatccga agaccggcgc caccagcgac atgatgcaga acggcatggg
     1441 gtatgcctcg atcgccgttc ccggcgaccc gcgcgtgaca ttccaggacg ccgcggaaga
     1501 ggccgagatc ctgaccgaag tcaacaagtg gacgccctgg taccaacgac tcgggaagtg
     1561 cctggcggtt gtcgcactgc tggccgccgc tggtatcgat ccctcgtcgc tcaatcccgg
     1621 atcgttcacg acatcggccg gcgccgcgaa ggttctctat cgcgtgatct cacgcaaccc
     1681 gaatgtcgcg aaggacatcg ccagctgctt cggataggca tcgacgtcga ttgcggggag
     1741 agggaatgac atgttgggat ttcgttgccg actcctgcgg gttgtcgtgt tgggactgat
     1801 cgcgatctcg ggctccgtcg cgcttggcgt gacgggggcg gacgccgtag cggcgcaggg
     1861 agcgacgccg tcaggatcgt cgaactgcgt gaccgtggtc gaaccgaacg atcagccgcc
     1921 gcaggcaacc gatctcggga cggacgtggg ttgcgccaca tccccaaacg cgaatggtgg
     1981 ccaggatgtc ttcaagtgga cggtcgatga cgccggcgcg gcaaagcgct ggacgatctc
     2041 gacctccgcg atcccgggac aggccgtaaa gctcgaaatc tacacagtca cggtcgatac
     2101 gaacggtgtt gtcacgaacc ccgtgaagct taccggggtg agtggcgact ccggacaggc
     2161 ggcgtcgatc gagaacgttc tggtcgccaa gggcacgtac ttcatcgcga cggcaatcgg
     2221 cggcggtggc gcgtacgaac tcacggtgtc gccgggcgat tcggtgccgg cggcgaacga
     2281 caccggcgat catgcttcgc cggaaacagc cctgccgctg caggacgcct tcgcgctttc
     2341 gggcgatcgc gcaagcggtg tcgacggctt cgcgtggacg ctcgacgatc gggcggcggc
     2401 aacccactgg acgct
//
LOCUS       DASTMD010000447         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1916825, whole genome shotgun sequence.
ACCESSION   DASTMD010000447 DASTMD010000000
VERSION     DASTMD010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916825"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFQ54_06475"
     CDS             <1..487
                     /locus_tag="VFQ54_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU0164675.1"
                     /translation="SLRVGGNRFDESISNYIKRKYSLRVGERTAEEVKIAIGSAMQLE
                     EDKVMEVRGRDEVAGLPRTIQIHANEVTDAITEALEAIIGAVRAVLEETPPELSSDII
                     DKGMILTGGGALLRHLPDLLTEVTGVPCYVADDPLSCVAIGTGLALEHMDILRDSLEE
                     P"
     gene            810..1250
                     /locus_tag="VFQ54_06480"
     CDS             810..1250
                     /locus_tag="VFQ54_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU0164676.1"
                     /translation="MAGSPVASPATAASPAAVIPTTTSANQSPPAGGATPTSATPVAS
                     PEASPASGTPAASTVVTSCDPDVIPPFTGDSPDYVTSSDLNFRAGPGSDCDLIGDPLA
                     EGVALTVLSDPVTRQGEDGQWVQVNVDGQLGWVSSDYIEPASGN"
     gene            1372..2163
                     /locus_tag="VFQ54_06485"
                     /pseudo
     CDS             1372..2163
                     /locus_tag="VFQ54_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
BASE COUNT          382 a          683 c          705 g          393 t
ORIGIN      
        1 gtcgctacgt gttggcggca accggttcga tgagtcgatc agtaattaca tcaaacgcaa
       61 gtacagcttg cgagtcggtg aacgcacggc cgaagaggtg aagatcgcga tcggctcggc
      121 gatgcagctc gaggaagaca aggtgatgga agtgcgcggc cgcgacgaag tcgccggtct
      181 gccgcgcacg atccagattc acgccaacga ggtgaccgat gcgatcaccg aggctctgga
      241 ggcgatcatc ggcgcggtac gcgccgtgct tgaggaaacc ccaccggagc tttcttcgga
      301 tattatcgac aaggggatga tcctcaccgg tgggggggcg ctacttcgcc atcttcccga
      361 tcttttgacg gaagtgaccg gggttccctg ctacgtcgcg gacgacccgc tttcgtgtgt
      421 cgccatcgga acgggcctcg cgctcgagca catggatatc ctgcgggata gcctcgaaga
      481 gccataacat cgggagatgc cgcgatcccg ctcgcggcat gaggaattcg ctatccgcca
      541 tcaccgcacg gagggccacg caagcgatga agacccccag gaatcggttc aacttctggt
      601 tgaggggagc gtcaatcggt ctccttgcga tggcccttgc cggatgcggc tccatcggtg
      661 gcagtgattc caagccaacc gcggtcccga cgccaacggt ctggcccacc gttgcggtgc
      721 agaacgagcc cacggtgacg gcggccccag ggacgccgga tcttcattca tcgccaatcg
      781 caacgcccgc gaatcaacca acgaccccag tggcaggttc tcctgtcgcc agtccggcga
      841 cagctgcatc gccggcggca gtcattccaa cgacgacgtc cgcgaatcaa tcgccaccgg
      901 cgggcggggc gacaccgacc tcggcgacgc ctgtcgcgtc accggaggcg agtccggcat
      961 ccggaacgcc cgccgcctcc acggtcgtca ccagctgcga tccagatgtg attccaccgt
     1021 tcaccggcga cagcccggac tacgtcacct cgagcgacct gaacttccgg gccggtcccg
     1081 gttcggactg cgacctgatc ggtgatcccc tggcggaagg agtcgcgctg acggtgttga
     1141 gtgatccggt cacgcggcag ggcgaggacg gccagtgggt ccaggtgaac gtcgatggcc
     1201 agctcggctg ggtatcgagc gactacatcg aaccggcgag cggaaactga tccgccccca
     1261 accgatcggg gcgcctcgcg agatcactcg cggcgggcgc ccttttctcg ttgtcatggc
     1321 tgaccggtca ttcggatcgc ggcccaacag atgcatggag gatcgatccc gatggtgagt
     1381 tttccgcgag agagcggcgt cctgcttcat ccaacctcgt tgcctggccc ggacggcgtc
     1441 ggtgacttcg gtccggcggc gttccgcttc gtcgaatggc tggcgcgtgc cgggcaacga
     1501 ctctggcagg tgatgccgct caatcccacg ggattcggcg attctcccta ttcgtcgccc
     1561 tcggcgtttg ccgggaaccc gctgatgatc tccctcccct ggctacaggg cgagggtctc
     1621 ctgacggagt ccgacttcga ggatcgtccg cttttcccgg acgaccggtg cgacttcgcg
     1681 aatgccgtcc cgtacaagat gcgactcctg cgcgccgcgt tcgaccggtt tcgacgcggg
     1741 ggtgggtgag cgcagcatca ggaattcgag cggttctgcc aggagcagca gcactggctc
     1801 gacgatttcg ccgccttcat ggcgatcaag gaggcacacg gcggatcggg ttggcgatct
     1861 tgggaggagc caattcgaag acgcgcaccg gaggcgatcg ccgactggac gcgactcctc
     1921 gcgtcggaga ttcgtctcca caagttcatg cagtttcagt tcgaccggca gtggggtgtg
     1981 ctgcgggcgc atgcgaacag cctcggcatc cgcgtcgtcg gcgacgtgcc gatcttcgtt
     2041 gccgacgaca gtgcggatgt ctggacgaat ccccatctgt tcaaattcga tgtcgagggg
     2101 aacgcggtcg aagttgccgg agtgccgccg gatccgtttt ccgcgacggg tcagatctgg
     2161 ggc
//
LOCUS       DASTMD010000448         4171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1024861, whole genome shotgun sequence.
ACCESSION   DASTMD010000448 DASTMD010000000
VERSION     DASTMD010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024861"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFQ54_06490"
     CDS             complement(<1..299)
                     /locus_tag="VFQ54_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0164677.1"
                     /translation="MFRLDGKTALITGAGSGIGRQIALLFGKQGAHVTIGDLNDSAAQ
                     AVADEIMTGGGSANALHLDVTQLDSANAAVSSVAAERGHLDILVNNAGIGMVGNLT"
     gene            599..988
                     /locus_tag="VFQ54_06495"
     CDS             599..988
                     /locus_tag="VFQ54_06495"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEU0164678.1"
                     /translation="MSFFGTHHHEGNLVSLRDSAMTIANRDDDIRGRHVVDANDGEIG
                     WINDLLLGDDAGEISYLDVTCGWFFGYWRSHELIPVIDILDIDDETVHIDRAAGEHPH
                     LPAYHPALTEHTEQHEERHGLRRFMPL"
     gene            1230..1601
                     /locus_tag="VFQ54_06500"
     CDS             1230..1601
                     /locus_tag="VFQ54_06500"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEU0164679.1"
                     /translation="MTVASEADDIRGSRVVDRHGAFLGRLADFVLAEHHEGIAYLDIE
                     SGGFMGFHARHSLIPVGAISAIDGETVHIDPAHPRVTPYQPELAPKRIAWWNRTGSRK
                     IEPLYLIQRSNGGWSQIGPRV"
     gene            complement(1620..2027)
                     /locus_tag="VFQ54_06505"
     CDS             complement(1620..2027)
                     /locus_tag="VFQ54_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164680.1"
                     /translation="MGPQRASVVYVEPLLGKLKKDMFADADWSVSLHRFRGDVDATVD
                     QVRADDPDLVVLDYFFRDEDAAESLIRQLQAVESPAPIPILVCRERDQSDQALRQLLP
                     ADSARIITRPYTVEEFMGAARDLVAGREHQADI"
     gene            complement(2096..3289)
                     /locus_tag="VFQ54_06510"
     CDS             complement(2096..3289)
                     /locus_tag="VFQ54_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEU0164681.1"
                     /translation="MAELPPVGTRRFSTGGESPLSNQDRLQLALEAGALGLWDIDVST
                     RTIRGTASSRLADRSQTSFIFPLNDFLNMVHDDDRAIVSHALHKAMEQAEVSYATYRI
                     TRPDGEHRWIEAQTYPITEDDGTVRRVVGLTRDVTELRDVEGERRRLLEESLEARAAA
                     ERSVRQRDNFISSTIHDLRTPLTYISARAQLIQRRLRRSSIEPDEQRWIDEGLAQIGD
                     GVRQMIDLLSDLQDITFLQIGRPIELNLITTNLGPFVEEIATQMTQLFPEHQVTLHAG
                     SSPIPVEIDLIRMRRVVSNLVTNAAKYSDDGTTIDIELAADADWAFVSVADQGVGIPP
                     EELETIFERYRRGSNVESDHAGSGIGLAGARLITRQHGGELNVTSRLGVGSRFQLRLP
                     LRPGT"
     gene            3534..3659
                     /gene="ykgO"
                     /locus_tag="VFQ54_06515"
     CDS             3534..3659
                     /gene="ykgO"
                     /locus_tag="VFQ54_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEU0164682.1"
                     /translation="MKVRNSLRAAKNRSMTSQVVRRRGRVYIIDKRNPRFKARQG"
     gene            complement(3761..>4171)
                     /locus_tag="VFQ54_06520"
     CDS             complement(3761..>4171)
                     /locus_tag="VFQ54_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164683.1"
                     /translation="IRDRAEMRPSTMLWIGIDGIERNDGDLRGNFGDRLRRSIAASIH
                     ESIGGHPIVGCFDDEAIVACLPGVQKEEAGTYAAELERDLAKRMGATVARSVTIVVTR
                     CEANDSLERAMGRAKRMMLEIRRARPIAGFPTGV"
BASE COUNT          781 a         1269 c         1327 g          794 t
ORIGIN      
        1 gtgagattgc cgaccatccc gatcccggca ttgttgacga ggatatcgag gtggccccgt
       61 tcggcggcga cgctggagac ggcggcattt gcggagtcga gctgggtgac atcgagatga
      121 agcgcattgg cggagccgcc accggtcatg atctcatcgg cgaccgcctg ggcggcgctg
      181 tcgttgagat cgccgatcgt cacgtgggcg ccctgtttcc cgaagaggag tgcgatctgg
      241 cgtccgatgc cggaaccggc gccggtgatg agagcggtct tgccgtcgag tctgaacacg
      301 atgcctcccg agattcgcct gcgagatcat gaagcggacg gacgcggtcc gctcgggcca
      361 tggggcggga tcggacgcgg gactgtggaa cgcaattgta tacaattgtg caggaatggg
      421 aaccagaatc aggaatgagt tgcaggatcg gaggacgaag gcgaggactc tcgcccaccg
      481 actaggccgt gcgctgtctg agtggtggca tgataattgc agtactgcaa gttatctacc
      541 aacgtccaga cgaccggcgt atccgacgga ggagcgccac gacgcaaagg agctcgggat
      601 gtcgtttttc ggtactcatc atcatgaggg gaatctggtg agcctgcgcg actccgcgat
      661 gacgatcgcg aatcgcgacg atgatatccg tggtcgccac gtcgtcgatg cgaacgacgg
      721 ggagatcggc tggatcaacg acctgcttct gggggacgac gcgggtgaga ttagttacct
      781 ggacgtaaca tgcggatggt tcttcggcta ctggcggtcg cacgagttga tccccgtgat
      841 cgacatcctc gatatcgacg acgagaccgt tcatatcgat cgagccgccg gcgaacatcc
      901 gcatctgccc gcgtatcatc ctgcccttac cgagcacacc gaacagcacg aggagcgcca
      961 cggcctccgg aggttcatgc cgctctgatc cgagcgggtg ctgcaccatt gagggcgaac
     1021 cagggcgctc cggaggtggg acgtgtatgt cctgcctccg gagcgatcgc gttgccggag
     1081 gccagccgcg gcgggataga cttttgtgct ggatggatta caattagaag aactggagca
     1141 cgagagcaac gaagctctcg aaaaacgcac aggggcaagc aatggatacc taacaaccac
     1201 gcaggacgtt tctgctcagg gacaccgcga tgaccgtcgc cagcgaggcg gatgatatcc
     1261 gcggcagccg ggtggtcgat cggcacggtg cgttcctcgg tcgcctcgca gatttcgtgc
     1321 ttgccgagca tcatgaaggt atcgcctacc tcgacatcga gagcggtgga ttcatggggt
     1381 ttcacgcccg gcattcgctg atcccggtcg gagcgatctc tgcgatcgat ggtgagaccg
     1441 tccatatcga cccggcccac ccccgagtga cgccctatca acccgagctt gcgccgaagc
     1501 gaattgcctg gtggaaccgg accggctcgc gcaagatcga gccgctctat ctgatccagc
     1561 ggagcaatgg tggctggtca cagatcggtc cacgcgtctg aggtctcgac cgacgcgatt
     1621 cagatgtcgg cctggtgctc acgccctgcg acgagatcgc gcgccgcgcc catgaattcc
     1681 tcgacggtgt atgggcgtgt gatgatccgc gcggaatcgg cggggaggag ttgccgcaac
     1741 gcctggtccg actgatcccg ttcccggcag acgaggatcg ggatcggtgc tggcgactcg
     1801 accgcctgca actgacggat cagtgattcc gcggcatcct catcccgaaa gaaatagtcc
     1861 agaaccacga ggtccggatc atcagccctc acctgatcga ccgtcgcgtc gacgtccccc
     1921 cggaaccgat gcagcgagac ggaccagtcg gcgtccgcga acatgtcctt cttgagtttt
     1981 ccgagcaacg gctcgacata gacaacactc gctcgttgcg gacccatgat ttcctcccgg
     2041 ttcttacggc ttcggtgcgc gagtcgcttc ctgcgagtcg atcgtgcgcg aaacctcatg
     2101 tccctgggcg caacggaagc cggagctgga atctgcttcc cacgcccaac ctactcgtga
     2161 cattcagctc gccgccgtgt tggcgagtga tgagccgagc acccgccagt ccgatgccag
     2221 agccggcgtg gtcggactcc acattggagc ctcgacgata tcgttcgaag atcgtttcca
     2281 actcttccgg cggaatcccg acaccttggt ccgcgaccga cacgaatgcc caatctgcgt
     2341 ccgccgccag ctcgatgtcg atcgtcgttc cgtcgtcgct gtatttcgcg gcattcgtca
     2401 cgagattgga gacgaccctc cgcattctga tcagatcgat ctcgaccgga atcggggacg
     2461 agccggcgtg cagggtcacc tgatgttccg ggaagagctg cgtcatctgg gtggcgatct
     2521 cttccacgaa cggcccgaga ttggtcgtga tgagattgag ttcgatcggg cgtccgatct
     2581 ggaggaacgt gatgtcctgg agatcggaga gcagatcgat catctgccgc actccgtcgc
     2641 cgatctgggc gagcccctcg tcaatccatc gctgctcgtc cggttcgatc gaggagcggc
     2701 ggaggcgccg ctggatcagc tgcgcgcgcg ccgagatgta ggtcaacggc gtccgcaggt
     2761 cgtgaatggt cgaggaaatg aagttgtcgc gctggcggac cgatcgctcg gcggcggccc
     2821 tcgcctcgag cgactcctcg aggagtcgcc ttcgctcgcc ttcgacgtcc cgaagttcgg
     2881 tgacgtctcg cgtgagaccg accacccggc gcacggtgcc atcgtcttca gtgatcggat
     2941 acgtctgtgc ctcgatccag cggtgctcgc cgtcgggacg cgtgatccga taggtcgcgt
     3001 aggaaacttc tgcctgctcc atcgccttgt ggagcgcatg ggaaacgatc gcccgatcgt
     3061 cgtcgtgaac catgttcagg aagtcattca gcgggaagat gaaggacgtc tggctccgat
     3121 cggccaggcg cgaggatgcg gtgccgcgaa tcgtgcgcgt ggacacatcg atatcccaaa
     3181 gcccgagggc gcccgcctcg agcgccagct ggaggcgatc ctggttcgag aggggtgatt
     3241 cacccccggt cgagaatcgt cgtgttccca cgggaggcag ttccgccatt cgacgatcct
     3301 tctgaatact gtcgcggtgc cgcggggcga acgatcggac gcttcggatg tgatgcatcc
     3361 aataaactat gcccggatcg accttgtcca tagcgcgttc cggccatgat gcaggcgtac
     3421 ctggtgcgtg atatgaaccg gatcaggtag cgacgcagcg ccagtgactt cgatatcgta
     3481 tagatactgg gtctcatcta tgatatactg gacccataga gaaggagggt gccatgaagg
     3541 tacgaaattc gttgcgggcc gccaagaacc ggagcatgac gagtcaggtc gtccggcgcc
     3601 gcgggcgtgt ctacatcatc gataagcgca atccccggtt caaggctcgt cagggctaaa
     3661 ccggacacga caacgccccg cgtcaacgac gacgcggggc gttgtcgtgt ccggcccctg
     3721 gtcgcaacgt ggcgtcaagc cggcgagcgg cgcgacgacc ttacactccg gtcgggaatc
     3781 cggcgatcgg ccttgctcgc ctgatttcga gcatcatccg tttcgcccgc cccatcgcgc
     3841 gttcgagcga gtcgttggcc tcgcatctcg tcacgacgat ggtcaccgag cgcgcgaccg
     3901 tggcccccat gcgttttgcc agatcgcgct ccagctccgc cgcgtacgtg cctgcttcct
     3961 ctttctgaac cccgggcagg caggcgacga tcgcctcgtc gtcgaagcag cccacgatcg
     4021 gatgcccccc gatcgactcg tggatggacg cggcgatgga tcgccgcagg cggtcgccga
     4081 aattgccccg gagatcgcca tcatttcgtt cgataccgtc gatcccgatc cagagcatgg
     4141 tggatggccg catctctgcc cgatcgcgga t
//
LOCUS       DASTMD010000449         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3168392, whole genome shotgun sequence.
ACCESSION   DASTMD010000449 DASTMD010000000
VERSION     DASTMD010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3168392"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1088
                     /locus_tag="VFQ54_06525"
     CDS             57..1088
                     /locus_tag="VFQ54_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HEU0164684.1"
                     /translation="MDLWETDRLLAPALQPGDTIGIISPSWFGGTDFIPRAESGLATL
                     HALGFRTRVAPHAFNNAGTVSDSAANRVEDLHAMFADPEIDAIICTIGGNHSCHLLPL
                     IDWDLIRANPKVFMGFSDITVLNVAIWVMTGLVTFNGPSLLTDWAEYPEMPPFSRDLA
                     LRAIASTESIGALASAPEWTDEFLDWVTGEDRIHRRLHTPSTGWRWLREGRAEGVLVG
                     GCLESLQHLRGTPYWPDLDGAILFLETSEEKPSPSDVDALLMDFENMDVFAKLSGLIL
                     ARPYGYDPDAVRQLHQVVIERTGKFGFPVLADVDCGHTTPLITLPIGCAAELDSDFDR
                     FAITEPAVR"
     gene            1111..1875
                     /locus_tag="VFQ54_06530"
     CDS             1111..1875
                     /locus_tag="VFQ54_06530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0164685.1"
                     /translation="MTSVSSFYDELAADYHLNYADWNNAVRRQGRTLAQLIAANGAAG
                     ARTVLDCSCGIGTQAIGLALEEYAVTATDISPASIERARHEAGNFGVEIPFSVADFRD
                     LSSVETTFDAVISCDNSLTHVLTDDDILTALRSMRDRLNPGGLLVIGIRDYDEITRQR
                     PRSTSPQVIDRETGRSILFQVWDWFEDGRGYDFTLFVVKAEGEGWATTAKTTRYRALL
                     RDELDALLLEAGFVDPEWHFWEETGHHQPLVTARRA"
     gene            complement(1943..>2241)
                     /locus_tag="VFQ54_06535"
     CDS             complement(1943..>2241)
                     /locus_tag="VFQ54_06535"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU0164686.1"
                     /translation="IITAAGLIMVLVFAGFILGDELTIKLLGLGLATAVLLDAFIVRT
                     MLVPAIMHQLGAANWWYPAWLDRITPHVSIEAPEEIIAPDEVMPMASGKKAGTT"
BASE COUNT          398 a          672 c          732 g          439 t
ORIGIN      
        1 acgaacgtgt cgtgtcaggc acgcgttctt gcgctcatcg tgaagcgaga aacgacatgg
       61 acctctggga aacggatcga cttctggctc cggccttgca gccgggtgac acgatcggga
      121 tcatcagtcc gtcgtggttc ggtgggacgg atttcattcc tcgtgccgag tctggactcg
      181 cgacgctcca cgcgctgggc tttcgaacga gggtggcccc gcacgctttc aacaatgcgg
      241 gtacggtttc ggattccgca gcgaaccggg tcgaggatct gcacgcgatg ttcgccgatc
      301 ccgagatcga cgcgatcatc tgcacgatcg gcggcaatca ctcgtgccat ctgctgccgc
      361 tgatcgactg ggatctgatc cgcgccaatc cgaaggtctt catggggttc tcggacatca
      421 ccgtcctcaa tgtcgcgatc tgggtgatga ccgggctcgt gacattcaac ggaccttcgc
      481 tgctgaccga ttgggcggag tatccggaga tgccgccgtt ctcacgcgat ctcgcattgc
      541 gcgcgatcgc gtcaaccgaa tcgatcggtg cgctcgcctc cgcgccggag tggaccgatg
      601 agtttctgga ctgggtcacg ggcgaggatc ggattcatcg aagattgcat acgccttcaa
      661 cggggtggcg ctggttgcgg gaaggtcggg cggagggggt gcttgttggt ggctgcctgg
      721 agagtctgca gcatctgcgt gggacgccgt actggccgga tctcgacggg gcgatcctct
      781 tcctcgagac ttcggaggag aaaccctccc cgtcggatgt cgatgctctc ctgatggact
      841 tcgagaacat ggatgtcttt gctaagcttt ccggactgat tctggcgcgc ccgtacgggt
      901 atgatccgga tgccgtgagg cagcttcatc aggtagtcat cgagcggacg gggaaattcg
      961 gttttccagt gctggcggat gttgattgcg gccacacgac gccgttgatc acgcttccga
     1021 tcggctgcgc cgcggaactc gattccgact tcgatcgatt tgccatcacc gaaccagcgg
     1081 tgcgctagac ccaatgggga agggacagga atgacttcgg tttcgagctt ttacgatgaa
     1141 ctcgccgcgg attaccacct caattacgcc gactggaaca acgcggtcag gcgacagggc
     1201 aggacgctgg cccagctgat cgcggcgaat ggcgccgcgg gtgcacggac ggtgctcgac
     1261 tgctcgtgcg ggatcgggac gcaggcgatc gggctcgcgc tcgaggagta cgcggtcacg
     1321 gccaccgaca tcagtccggc ctcgatcgaa cgggcgaggc acgaggcagg gaacttcggc
     1381 gtcgagattc ccttcagcgt cgccgatttc cgggatctct cgtcagtaga gacgacattc
     1441 gatgcggtga tttcctgcga caactcgctc acgcacgtcc tgaccgacga cgatatcctg
     1501 accgcgttgc gcagcatgcg cgatcgcctg aatcccggcg gcttgctggt gatcggaatc
     1561 cgcgactacg acgagatcac gcggcagcga ccgcgatcga cgtcgccgca ggtgatcgat
     1621 cgggagacgg gtcgctcgat cctcttccag gtctgggact ggttcgagga cggtcgcggc
     1681 tacgacttca cgctcttcgt agtaaaggcg gagggggaag gctgggccac gacggcgaag
     1741 acgacgcgct accgcgcact gctccgggat gaactcgacg cgcttctgct cgaggccggt
     1801 ttcgtcgatc ccgaatggca tttctgggaa gagaccggtc atcaccagcc gctcgtcacg
     1861 gcgcgccggg cgtagctccc gtccgtcctc cgcccatctg cccgttggag gacgctcacg
     1921 gatggtcggg gactggcgga aactacgtcg tcccggcctt cttcccgctc gccatcggca
     1981 tgacctcatc cggcgcgatg atttcctccg gggcctcgat cgagacatgc ggggtgatcc
     2041 tgtccagcca ggccgggtac caccagttgg cagccccgag ctgatgcatg atcgccggca
     2101 caagcatcgt gcggacgatg aatgcgtcca ggagtaccgc ggtggccagc ccgaggccaa
     2161 ggagcttgat cgtcagttcg tccccgagga tgaatccggc gaaaacgaga accatgatca
     2221 gaccggcggc cgtgatgatg c
//
LOCUS       DASTMD010000450         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2274862, whole genome shotgun sequence.
ACCESSION   DASTMD010000450 DASTMD010000000
VERSION     DASTMD010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2274862"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1550)
                     /locus_tag="VFQ54_06540"
     CDS             complement(318..1550)
                     /locus_tag="VFQ54_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0164687.1"
                     /translation="MTATTISAITSRDRRFPLKPGEGSDAVHTDPTYAYAVTELHTTS
                     GVTGIGLAFTLGGGNELVTRAIDLLAAGLVGREIESLMADFGETQRQLANHPQLRWLG
                     PHQGVIHLALSSITNACFDLWAKSRGVPLWQLLLDLSPEELVATLDLSYLEDVLTADD
                     AIQIIATPQATRGQRLPILDEGYPGYDTSAGWFGYSDEKIRANAARAVANGFTALKLK
                     VGHKDPRRDIARARLVRTTVGPGIRLMVDANQQWTLPQALAICADLADLDLFWIEEPT
                     HPNDVLAHQTLAQAIAPTRLAIGEHVANRIGFKNYLQAGAAGFIQVDCTRVAGVSEFL
                     VVSLLARKYGVPIVPHVGDMGQIHQHLVLFNHIALDHEALFLEYIPHLREHFTDPAIV
                     EDGRYRTPRTPGSSSDLR"
     gene            complement(1547..>2063)
                     /locus_tag="VFQ54_06545"
     CDS             complement(1547..>2063)
                     /locus_tag="VFQ54_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164688.1"
                     /translation="AMIGDWPGSLGLIADPAVSAVADRFDVALYPTGPGGLRRVYSGS
                     HSFAIAKACQDVDAALTLLRDLTSFDAQLAEAQHGAVPARSDAADRNRELTTSPLARR
                     IQDLQAETIATSMISFPPLAWYPLLEEIVWPTLAAVLRDELSVNDAIEQAQSTLDADP
                     RITTPEALPHA"
BASE COUNT          336 a          619 c          762 g          346 t
ORIGIN      
        1 tgttgcccgc ccgccgggac gtactggcga tcgtcatcgc agatgcggca tcgcgtcggc
       61 gggcgtgacg actgcgggta ctgcgtcccg caggtctggc agagccaggc gatcggttcg
      121 ttcgaaggag cgggtgcatt cgtggtggcg gcaaggatcg tcgtcacgtg gagctcctat
      181 ccggcggcgc ggcgcggcgt gcaggcaggc ggggatcgat ccgctcatcc gctcatgata
      241 cggcggcgat ggcccggtga aacatgtacg catgtcccaa tctttcatcc gcgcgactgg
      301 cccccgcccg ctcccgatca gcgcaggtcg gaggaggagc ccggggttcg tggcgtccgg
      361 tagcgaccgt cctcgacgat cgcggggtcg gtgaaatgct cccggagatg cgggatgtat
      421 tcgaggaaga gcgcctcgtg atcgagcgcg atgtggttga agagcacgag gtgctggtgg
      481 atctgcccca tgtccccgac gtggggcacg atgggcacgc cgtacttccg cgcgaggaga
      541 ctgacgacga ggaattcgct gactcccgcg acgcgcgtgc agtcgacctg gatgaagccg
      601 gcggcacccg cctgcaggta gttcttgaag ccgatccgat tcgctacatg ctcgccgatc
      661 gcgagacggg tcggcgcgat cgcctgcgca agcgtctggt gggcgaggac gtcgttcgga
      721 tgcgtcggtt cctcgatcca gaagagatcg aggtcggcga gatcggcgca gattgcaagc
      781 gcctggggga gcgtccactg ctggttggcg tccaccatca gccgaatgcc ggggccaacg
      841 gtcgtccgga cgagacgggc gcgggcgata tcccgtcgcg ggtccttgtg gccgaccttg
      901 agcttgaggg cagtgaagcc gttggcgacg gcacgcgccg cgttggcgcg gatcttctcg
      961 tcgctgtagc cgaaccagcc ggcggaggtg tcgtagccag gatatccctc gtcgagaatc
     1021 gggagcctct gcccgcgggt tgcctggggc gtggcgatga tctggatcgc gtcatcagcg
     1081 gtcagcacat cctcgagata ggagagatcg agcgtcgcga cgagttcttc tggtgaaaga
     1141 tcgagcagga gttgccagag cggaacgcct cgcgacttcg cccagagatc gaagcaggcg
     1201 ttcgtgatcg acgagagcgc gaggtggatg accccctggt gcggaccgag ccaacgcagt
     1261 tgcgggtggt tcgccagctg tcgctgcgtc tccccgaaat ccgccatcag cgactcgatc
     1321 tcccgcccca ccagacctgc cgcgagcaga tcgatcgccc gggtgacgag ctcattcccg
     1381 ccgccgagcg tgaaggcgag accgattccg gtcacgccgg acgtcgtgtg gagttcggtc
     1441 accgcgtagg cgtaggtcgg gtcggtgtga acggcgtcgg agccttcgcc cggcttcagc
     1501 gggaagcggc ggtccctgga cgtgatggcg gagatcgtgg tggcggtcat gcgtgaggga
     1561 gagcctccgg ggtcgtgatg cgggggtcgg cgtcgagcgt cgattgcgcc tgctcgatgg
     1621 cgtcgttgac ggagagttcg tcgcggagaa cggcggcgag cgtcggccag acgatctctt
     1681 cgagcagcgg ataccaggcc aggggtggaa acgagatcat cgacgtcgca atcgtctccg
     1741 cctggagatc ctggatccgg cgcgccagcg gcgatgtcgt gagctcgcga ttccggtcgg
     1801 ccgcgtcgct tcgggcggga acggcgccgt gctgcgcctc ggcgagctgt gcatcgaaac
     1861 tcgtcaggtc gcggagaagc gtgagcgccg catcgacgtc ctgacacgcc ttcgcgatcg
     1921 cgaacgagtg cgatccggag tagacgcgcc ggagaccgcc ggggccggtc ggatagagcg
     1981 cgacatcgaa gcgatcggcg accgcggaaa cggccggatc ggcgatcaat ccgagcgatc
     2041 ctggccagtc gccgatcatc gcg
//
LOCUS       DASTMD010000451         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2185660, whole genome shotgun sequence.
ACCESSION   DASTMD010000451 DASTMD010000000
VERSION     DASTMD010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185660"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..1459
                     /locus_tag="VFQ54_06550"
     CDS             302..1459
                     /locus_tag="VFQ54_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164689.1"
                     /translation="MVDPGTGRESGSKRMVSSRARLWAAPAIAAVVFGGRLGAIGAQT
                     PAATPGSSPVAAGSADLSGLKSYVVEHVASLTRGASDLQTFSQGYYDLLKAANFDYQA
                     TWDANKATLEPSFDQARTYFTEDAHGNYEMVEGLVAGLPDLVQYDVWLDAGPTGAEDP
                     TNARQWTLTLPDGRTFENPGNLFHSLLEPTLWCTDTQFIGLALDTNGDGRTAIPDGMP
                     DANFCLGLSQVLVQAVGELSASVDAWEPTLSDAFTALVVMLPTAEGYFNDWKLSPSVL
                     GDKSTQTGFVANSRLLDVLGIYGGLKLTYDKTGPEVAKASIELANQIQTELDSLVGFV
                     QGLYDHEKAGTRYTPEQADQFGTEVQSRAESLAGQITQAAALMDVEIKDVG"
     gene            1533..3917
                     /locus_tag="VFQ54_06555"
     CDS             1533..3917
                     /locus_tag="VFQ54_06555"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEU0164690.1"
                     /translation="MRIRLRLLMLCAYLSMLAPAGLLLPGGSSAAPLAIDPGTTGLAI
                     HSTFFDAQQDLLLGDPATARMDIAKAVPLVQQFAAALVDDRAAATTITDGMTAATAAV
                     AANDQIGLASARGRVWTAMLAGAYHEVLASVRANDAKSAASWLLVREFRPTTKFARPG
                     ADATLAVKSLGAGTMTPDQAVLAVNADLLDTYQSKLDTELTVLSAAADQKFGARQAES
                     AATIHGYWSILSSAYQDQLGASQLETANATVTQLLTSVSSGDAAQIDASVTAVTSLSR
                     SFRAAPLSDDEKARRAGQLMRYLSLVPVEYGRGVKNGTVILDIEIQEAQTFLTASQAS
                     FDDLHLYLEQQDKAKTDQVEGILDNLDRQITAAANHTEVASPDDIRSQISDVSDTLDS
                     MFPSEWTRSGGDADFDVIGSLLDQMEVDIKAGNWDQAESARLEAYAIYEVGAEKRLLA
                     FAPDLANRTEQLFWQGTGSTPGLAVAIQQKKPLPEIQKSRNELDVALADGQERLGAGR
                     PETIVVIFNAATIVFREGLEAVLILASLIASMVGANQKFKKPLALGALAALLATAGLF
                     ILADTLLGSLAKYGEKLEAIVSLIAIAILLVIMNWFFHKVYWTRWIGAQHKRRRAAIG
                     VATGQVVGLVTLGFTSIFREGAESVLFLQALVLDAGAWVVIQGTLLGLGGTAVVGVLV
                     FALQKKLPHKKMLMVTGGMIALVLVVMVGNTVHVMQIVGWLPITAIPGWELPYWVGQW
                     FGVYPIWESLVLQALSLVVVLGSYFGAEFVNKREQRKLIAIAKAEQQSARPAAG"
BASE COUNT          745 a         1364 c         1371 g          785 t
ORIGIN      
        1 ttgatgcctg gttgcgccga acggtccgtc cctttcgcct cgcgacggac tgcctctgga
       61 actcacgcac cctcaatgtc cgagaatgat ttccgaaaag acatacgtgt ttgataaaag
      121 acgacgatat ttttctttct ccttgacacc caatgattcc gaccgctagt atccggattg
      181 tcaattctga cctgtttcgt atgaattgag atggcgtttc aagagactgc ttttcagcct
      241 ttgcagatgc atgcgcgacg tgggttcgga gagaatgcac gttcgtcggg gagagctacg
      301 ggtggtggat cctggaaccg gtcgcgaaag cggatcgaag cggatggtct cgtcgcgggc
      361 gcggctgtgg gcggcaccgg cgatcgctgc cgtcgtcttt ggcggtcgcc tcggggcgat
      421 cggcgcgcag acaccggcgg cgacaccagg ttcgtcaccg gtcgccgcag gcagtgccga
      481 tctttccggc ctcaagtcct acgtcgtcga gcatgtcgcg tcgctgacca ggggagcgag
      541 cgatctgcag accttctcgc agggctacta cgacctgctc aaggcggcga atttcgatta
      601 tcaggccacg tgggatgcca ataaggcgac gctcgagcca tcgttcgacc aggcgcgcac
      661 ctacttcacg gaggatgccc acggcaacta cgagatggtc gagggtctcg tcgccggtct
      721 tcccgatctc gtgcaatatg atgtctggct tgatgctggt cccaccggcg ccgaggatcc
      781 gacgaacgcc aggcaatgga cgctcacgct gcccgacggc cgcaccttcg agaaccccgg
      841 caatctcttc cattccctgc tcgagccaac cctctggtgc accgacacgc agttcatcgg
      901 gctggcgctc gatacgaatg gcgatggcag gaccgcgatt cccgacggga tgccggatgc
      961 gaacttctgc ctcgggcttt cgcaggtcct cgtacaggcg gttggtgagc tcagcgcctc
     1021 cgtcgacgcg tgggaaccga cgctctccga tgccttcacg gcgctcgtcg tgatgctgcc
     1081 gacagccgag ggctacttca acgactggaa gctctcgccc tccgtccttg gggacaagag
     1141 cacgcagacg ggtttcgtcg cgaacagtcg cctgctcgat gtgctcggca tctacggtgg
     1201 attgaagctc acctacgaca agacgggtcc cgaagtcgcg aaggcatcga tcgaactggc
     1261 gaaccagatc cagaccgaac tcgacagcct cgtcggcttc gtgcaaggac tctacgatca
     1321 cgagaaggcg gggacgcgct acacgccgga gcaggccgac cagttcggaa ccgaggtcca
     1381 gtcgcgggcg gagtcgctcg ccggccagat cacgcaggcc gcggcgctca tggacgtcga
     1441 gatcaaggac gtcggctaga ccgcacgcaa tgacgtatcg cgccggggat tctggtcccc
     1501 ggcgcttcgt tggtttcacg aggatggatc gaatgcggat ccggttacga ttgctgatgc
     1561 tgtgtgcgta cctgtcgatg cttgcgccgg caggtctttt gctgcccggg ggatcgtccg
     1621 cggcgccgct cgcgatcgat ccggggacca ctggccttgc gattcactcc actttcttcg
     1681 atgcccagca ggatctcctg ctcggggatc cggcgaccgc caggatggac atcgcgaaag
     1741 cggtcccgct cgtccagcag ttcgccgcgg cactcgtgga tgacagggcc gctgccacca
     1801 ccatcaccga tggcatgact gccgcgacgg cggcggtcgc cgccaacgac cagatcggcc
     1861 tcgcctccgc gcgcggtcgc gtctggacgg cgatgctcgc cggcgcctat cacgaagtgc
     1921 ttgcgagcgt ccgcgccaac gacgcgaaat cggcggcatc ctggctgctt gtgcgcgagt
     1981 tccggccgac caccaagttc gcccgcccgg gcgccgacgc gacccttgcc gtgaagagcc
     2041 tcggcgccgg gacgatgact ccggatcagg cggtccttgc ggtcaacgcc gatctgctgg
     2101 atacgtacca gagcaagctc gacaccgaac tgaccgttct ttccgccgcc gccgaccaga
     2161 agttcggcgc gcgccaggcg gaatccgccg ccacgatcca cggctactgg tcgattctct
     2221 cgtccgcgta tcaggatcag ttgggcgctt cccaactcga gacggccaac gccacggtca
     2281 cgcaactcct cacatccgtg agttcgggtg acgccgcgca gatcgatgcg tctgtcacgg
     2341 ccgtgacgtc gctctcgcgc agcttccgcg cggcgccgct ctcggacgac gagaaggcgc
     2401 gccgcgcggg tcagctcatg cgctacctct cgctcgtgcc ggtcgaatac gggcgtggtg
     2461 tgaagaacgg cacggtcatc ctcgatatcg agattcagga agcgcagacc ttcctcaccg
     2521 cctcccaggc ttcattcgac gatcttcatc tctatctgga gcagcaagac aaggcgaaga
     2581 ccgaccaggt cgaggggatt ctcgacaacc tcgacaggca gatcaccgcc gccgcgaatc
     2641 acaccgaggt cgcgagcccg gacgacatcc gttcccagat ctcggacgtg agcgacacgc
     2701 tcgattcgat gttcccgtcg gaatggacgc gctcgggcgg cgacgccgac ttcgacgtga
     2761 tcggctcgct cctcgaccag atggaggtcg acatcaaggc cggcaactgg gaccaggcgg
     2821 agtcggcccg gctcgaggcg tacgcgatct acgaggttgg ggccgagaag cgacttctcg
     2881 ccttcgcccc ggacctcgcg aaccggaccg aacaactctt ctggcaagga accggatcga
     2941 cgccgggcct tgcggtcgcg atccagcaga agaagccact cccggagatc cagaagtcgc
     3001 gcaacgagct cgacgtggcg ctggcggatg gtcaggagcg actcggcgca gggcgaccgg
     3061 agacgatcgt cgtgatcttc aacgcggcga cgatcgtctt ccgcgagggg ctcgaggcgg
     3121 tactgatcct tgcgtcgctg atcgcgagca tggtgggcgc caaccagaag ttcaagaagc
     3181 cactcgccct cggtgcactg gcggcattgc tggcgacggc agggctcttc attctcgcgg
     3241 ataccctgct tgggtcgctg gcgaaatatg gcgagaagct ggaagcgatc gtttccctga
     3301 tcgcgattgc gatcctgctc gtgatcatga actggttctt ccacaaggtg tactggacgc
     3361 gctggatcgg tgcgcagcac aagcgccgcc gggcggcgat cggtgtcgcc acggggcagg
     3421 ttgtcggcct ggtcaccctc ggcttcacga gcatcttccg ggaaggcgcg gagagcgtgc
     3481 tcttcctcca ggcgctcgtg ctcgatgccg gcgcgtgggt cgtgatccag ggaacgctgc
     3541 ttggcctcgg cggaacggcg gtggtgggcg ttctcgtctt cgcgctgcag aagaagctgc
     3601 cgcacaagaa gatgctgatg gttaccggcg ggatgatcgc gctcgtgctg gtcgtgatgg
     3661 tgggcaacac agtgcatgtg atgcagatcg ttggctggtt gccgatcacg gcgatcccgg
     3721 gctgggagct gccctattgg gtcggccagt ggttcggcgt ctacccgatc tgggagagcc
     3781 tggtgctcca ggcgttgtcg ctcgtcgtcg tcctcggcag ctacttcggt gccgagttcg
     3841 tgaacaagcg cgagcagcgg aagctgatcg cgatcgcgaa ggcggagcaa caatccgcgc
     3901 gaccggcggc tgggtaggcg accaggatct cgccgcctga tggggatcgc gcgtcaatcc
     3961 cgtccccgcg gcccttgtgg ccgtcggact ccgacgtgcg cccggaccgc gagtgcaatc
     4021 ggtgccggac ggcttcatcg tcgatacggc gaaaaccgac atggttgccc cttgggggcg
     4081 tacttcgccc gagtccgctt tgcggcgggg tatgaaaccc cgccctacca accccagcat
     4141 cgtcaaaacg ggtcggcggt tctggatttc gatgtaaacg gggacaagcc cctcacaccg
     4201 agccatcacc aatcccgtcc tcacggccct tgtggccgtt gaactccgac gtgtgcccgg
     4261 agcgc
//
LOCUS       DASTMD010000452         3552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2720864, whole genome shotgun sequence.
ACCESSION   DASTMD010000452 DASTMD010000000
VERSION     DASTMD010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2720864"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFQ54_06560"
     CDS             complement(<1..183)
                     /locus_tag="VFQ54_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164691.1"
                     /translation="MARHLTQGLEHDINANAVVERLADDGLAELDRLAVHRHHVTERD
                     PFLDDVSRQSEVEEDVL"
     gene            151..660
                     /locus_tag="VFQ54_06565"
     CDS             151..660
                     /locus_tag="VFQ54_06565"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0164692.1"
                     /translation="MLEALRKVTSHTADIYAVATTQEEVGLRGAGTSAFGVDPDISIG
                     LDVTLAGDIPGATPDAYVTRLHEGAAIKIVDSSLLANPRLVEHLRSIAEARGITYQIE
                     ILPRGGNDAGAQQRVRAGNIAATISIPTRYLHSVNESASIADIDACVDLLAAFLEEAG
                     SQSYAYTLD"
     gene            887..2728
                     /locus_tag="VFQ54_06570"
     CDS             887..2728
                     /locus_tag="VFQ54_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164693.1"
                     /translation="MSQRPISPSTADPSIATSSESHPAQSLGDVAISRRNLVRTTAMV
                     AGLAAAGGAATNVLAAPANTPEPTRAASQTDVQTKVEVAIPLIPYGQPVTIDPHRTVN
                     WGPFWTLFPHVWSGLLRFDENGKVEPDLAASLDANENQTVWTAKLRDDIKFASGRAIT
                     AADFIASWKRALSPAALTPMSGFMAPIKGFAAFTAGKSTELGIVAKDAKTIEITLAAS
                     LSSFPYYLATFVYAVLDGDVLGKATGADAKDPLLSGAGAGAWQFTEFMDDDHLTMIPN
                     PNYWDDPSPSISKVTWTILSGDDAETIALEQYKSNVFASADVPLSQYDAVNGDATLKP
                     DVHSLASQGSTLAIGLDFNQAPFNDVRIRKAVAASIDRDAWAKNVWKESYAPASAFTS
                     PASAANTAYTSPKGIAYDVDQAKKWLSDAKFDPAKNATDIVYYQPATDSTDDQKRHAQ
                     LLKMIEDAIGLTIRQDTTLTADQIAAQAQDNGGRQFDIVWWWPDGDAGSLLAKIGTST
                     SPYMAGWFNWSKGNEKHDNEDPGKDSQSFDKYIADAAKNGSESGRNKNYKSAEQLLLD
                     NAVYIPLGYWVQRFVQKPWLQGTKQGPWSGRIPVRFDKDVIVKGKPS"
     gene            complement(2968..>3552)
                     /locus_tag="VFQ54_06575"
     CDS             complement(2968..>3552)
                     /locus_tag="VFQ54_06575"
                     /inference="COORDINATES: protein motif:HMM:NF020772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HEU0164694.1"
                     /translation="DTELFGHWWFEGVDWLKAVLRGLAASDVVDLTTASTFINEHDPE
                     RVMNLPESSWGSGGNHFTWLNVDTQWMWPVIYEAEARMESLVAANPNATGELRKVLDQ
                     TARELLLLQSSDWPFLVTTGQAKEYATQRFTDHVDRFNALATMAELGGPQDDRDREYL
                     SSLTERDNPFPDIDYRVFAERQGNAALTAGAAQR"
BASE COUNT          681 a         1200 c         1075 g          596 t
ORIGIN      
        1 gaggacatct tcctcgacct cggactgccg gctgacgtcg tcaaggaacg ggtctcgctc
       61 ggtgacatgg tgacgatgga cagctagacg atcgagctcg gcgagaccgt cgtcagcaag
      121 gcgctcgacg accgcattgg catttatgtc atgctcgagg ccctgcgtaa ggtgacgagc
      181 cacacggctg atatctatgc ggtcgcgacg acgcaggagg aagtcgggct tcgcggagct
      241 ggaacctccg ccttcggcgt cgatccggac atcagcatcg gtctcgacgt cacactcgcc
      301 ggggacattc ccggagcgac acccgatgcg tacgtgacgc gtctccacga gggcgccgcg
      361 atcaagatcg tggacagctc cctgctcgcg aatccgcggc ttgtcgagca tctgcgttcg
      421 atcgccgagg cgcgcggcat tacctaccag atcgagatcc tgccccgcgg aggaaatgac
      481 gccggcgcgc agcagcgcgt tcgtgcggga aacatcgccg ccacgatctc gattccgacc
      541 cgatatctcc actccgtgaa cgagagcgcg agcatcgccg acatcgacgc ctgcgtcgat
      601 ctcctcgcgg cattcctcga ggaagccggc tcgcagtcgt acgcgtatac cctcgactag
      661 ccacatgctg gaaggagcgg tcgtcggtcg ctccttcctc gccaatccgt ccacatcacg
      721 cgcagcaatc ggcagtagaa tcgtgtcggg atcttctcgc gactcccggt cggtctccgt
      781 aacctaatcg ccttcgagat cgttcttgag actgagagtc ctgcaggacg gtggacgtcc
      841 ggcaccccgt tccggtcatc gcccgcactt cggcgaagga gcagcaatgt cgcaacgccc
      901 gatttcacca tctactgccg atccatccat cgcgacctca tccgaatccc accctgcgca
      961 atcgctcggc gacgtcgcga tctcccgccg caacctggtc cggacgaccg cgatggtcgc
     1021 gggtctcgct gccgcgggtg gcgcggccac gaacgtcctc gccgcgccgg ccaacacccc
     1081 agagccaacg cgggcggcct cccagaccga cgtccagacc aaggtcgagg tcgcgattcc
     1141 actgatccca tacggtcagc ccgtcaccat cgatcctcat cgcacggtga actggggccc
     1201 gttctggacg ctcttccccc acgtctggtc cgggctcctc cgcttcgatg agaacggcaa
     1261 ggtcgaaccc gacctcgcgg cctcgctcga cgccaacgag aaccagacgg tctggaccgc
     1321 gaagctgcgg gatgacatca agttcgcgtc cggacgggcg atcaccgcgg ccgacttcat
     1381 cgcatcctgg aagcgagcgc tgtcaccggc agcattgacg ccgatgtcgg gcttcatggc
     1441 tccgatcaag ggtttcgccg ccttcaccgc aggcaagagc accgagctgg ggatcgtcgc
     1501 caaggacgcg aagacgatcg agatcacgct cgccgcctcg ctgtcgtcct tcccctacta
     1561 cctcgcgacg ttcgtctacg cggtgcttga cggcgatgtc ctcggcaagg cgacgggggc
     1621 tgacgccaag gacccgctcc tctcgggcgc gggcgcgggc gcgtggcagt tcacggaatt
     1681 catggacgac gatcacctca cgatgatccc gaatccgaat tactgggacg atccatcacc
     1741 ctcgatctcg aaggtcacgt ggacgatcct gagcggggat gatgccgaaa ccatcgcgct
     1801 cgaacagtac aagtccaatg tgttcgcctc ggccgacgtg ccgctctccc agtacgatgc
     1861 cgtgaacggc gatgccacgc tcaaacccga tgtccactcg ctcgcgtcgc aggggtcgac
     1921 gctggcaatc ggtctggact tcaaccaggc gccgttcaac gacgttcgca tccgcaaggc
     1981 cgtcgcggca tccatcgacc gcgacgcctg ggcaaagaac gtctggaagg aaagctacgc
     2041 ccccgcttcg gcgttcacct ctccagcgag cgccgccaac accgcgtaca cgtcgccgaa
     2101 gggaatcgcg tacgacgtcg atcaggcgaa gaagtggctt tcggacgcaa agttcgatcc
     2161 cgccaagaac gcgaccgaca tcgtgtacta ccagccggcc accgattcca ccgacgacca
     2221 gaagcgccac gcccagctac tcaagatgat cgaggacgcg atcggactga ccatccgcca
     2281 ggacacgacg ctgactgccg accagatcgc ggcacaggcg caggacaacg gcgggcgtca
     2341 gttcgacatc gtctggtggt ggccggatgg agatgcgggc tcactgcttg cgaagatcgg
     2401 gacgtcgacc tccccgtaca tggcgggttg gttcaactgg tcgaagggca acgagaagca
     2461 cgacaacgag gatccgggca aggactcgca gtcgttcgac aagtacatcg ccgatgccgc
     2521 caagaacggc agcgagagcg gtcgcaacaa gaactacaag agcgccgagc agctcctgct
     2581 cgacaacgcg gtatacatcc cgctcggata ctgggtgcag cggttcgtcc agaagccatg
     2641 gctgcagggt acgaagcagg gcccatggag cgggcgcatc cccgttcgat tcgacaagga
     2701 cgtgatcgtc aaggggaagc catcgtagag cggcgagcga agtcgttgcc ggcgggagca
     2761 cgatcctcgg gcgaacgcga ggtgggaaac accgtctccg tccccacggc gcttgtcgcc
     2821 gttccgtgtc ggtctgcgct ggccttgtct tcgcccttgg ccggatggag tccgcaccct
     2881 ggcggccgtc acaatcaggc agcgaagaag aggccccatt ccggggcctc ctcttcgttt
     2941 cgcccgctcc tgtttcacgt gacgacgcta ccgctgggca gcgcccgcgg tcagagccgc
     3001 gtttccctga cgttccgcga acacgcggta gtcgatatcg ggaaagggat tgtcacgctc
     3061 ggtcagagag gaaagatact cgcggtcgcg gtcgtcctgc ggaccgccga gttccgccat
     3121 cgtcgcgagg gcattgaagc gatcgacgtg atccgtgaac cgctgcgttg cgtattcctt
     3181 cgcctgaccg gtcgtcacca gaaacggcca gtcactgctc tgcaggagca gcaactcccg
     3241 cgcggtctgg tcgaggacct tccgcaactc gcccgtcgcg ttcggattcg ccgcaaccag
     3301 cgattccatg cgggcctccg cctcatagat caccggccac atccactggg tgtcgacgtt
     3361 caaccacgtg aagtgattgc caccggagcc ccagctcgac tcgggcagat tcatcacgcg
     3421 ctccgggtca tgctcgttga tgaacgtcga agccgtcgtg agatcgacga cgtccgaagc
     3481 ggcgagaccc cggagcacgg ccttgagcca gtcgacgccc tcgaaccacc agtgcccgaa
     3541 gagctcggtg tc
//
LOCUS       DASTMD010000453         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3077808, whole genome shotgun sequence.
ACCESSION   DASTMD010000453 DASTMD010000000
VERSION     DASTMD010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3077808"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VFQ54_06580"
     CDS             complement(<1..1120)
                     /locus_tag="VFQ54_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164695.1"
                     /translation="MTTTPAPRGAGTNPASLPGRLLNWIRRRPAMAILSLLVGALLAG
                     GSIVGVLAQALGPAAPSPAQGHAAVVAHGVGNIPSSGNRWRIFTQTATAGSKPTAISS
                     PSFIMTTGNPVLVTDTTSGYQQRLAGGEAFFAHAGQSIGVEPFGAPQQYYLIQLGIDD
                     TPPSSPAYYVSDAFTGDGVARDISLIRDVLDDQEAGQIPAGATPTLVLVLAGSLDVTA
                     SSGKSGSLKTGQAGTFTGDLTVMGTAAGTSYIAAYIGATIPEVATPVASPATPAPATP
                     IKAATVAPTKAVPTATTAPTKVPPTRTPAPKPSPTKVVTPKASPAASPGASPAARNPK
                     ADDDKDGLTNAQEAALGTDPENPDTDADGISDGDEVNVY"
     gene            complement(1368..1970)
                     /locus_tag="VFQ54_06585"
     CDS             complement(1368..1970)
                     /locus_tag="VFQ54_06585"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU0164696.1"
                     /translation="MARELAPLPYAFDALEPHIDAQTMEIHHDRHHQTYVTNLNNAVA
                     GTDLDSVSSIDLVKDLSQVPEAKRTAVRNNAGGDVNHTFFWELLTPGGSTAPTGALAA
                     AIESTFGSVDSLKEQVNAAGAARFGSGWTWLVVDGGALKVVSTANQDSPLSDGQTPVI
                     GIDVWEHAYYLKYQNKRPAYLEAIWNVINWDVAGKNFGAA"
BASE COUNT          425 a          736 c          874 g          461 t
ORIGIN      
        1 cgtagacgtt gacttcgtcc ccgtcggaga tcccgtcggc gtccgtatcg ggattctcgg
       61 gatcagtgcc aagcgccgct tcctgcgcgt tcgtgaggcc atccttgtcg tcatccgcct
      121 tggggtttcg agcggcgggc gaggcaccag gtgatgcggc gggcgatgcc ttgggcgtca
      181 cgaccttcgt cggcgagggc ttcggggccg gcgtccgggt aggaggaacc ttcgtcggcg
      241 ccgtcgtggc ggtcggcact gccttcgttg gcgcgacggt cgcggccttg atcggcgttg
      301 ccggggccgg ggtggcggga gatgcgaccg gcgtcgcgac ctcgggaatc gtcgccccga
      361 tgtacgccgc gatgtaggac gtcccggctg ccgtgcccat caccgtcagg tcgccggtaa
      421 aggtacctgc ctgacccgtc ttgagcgacc ccgattttcc gctgctggcc gtgacatcga
      481 ggctgcccgc gaggacgagc acgagcgtgg gagtcgctcc ggccgggatc tggccagctt
      541 cctggtcgtc gaggacgtcg cggatcaacg agatgtcgcg tgcgacacca tcgccggtga
      601 atgcatcgct cacgtagtag gccggagacg agggcggggt gtcatcgatc ccgagctgga
      661 tcagatagta ctgttgcggc gccccgaacg gctcgacgcc gatcgattga ccggcgtggg
      721 cgaagaatgc ctcacccccg gcgagccgct gctgatatcc cgacgtcgta tcggtgacga
      781 gcacgggatt gcctgtcgtc atgatgaagc ttggggagga aatggcggtt ggcttgctgc
      841 cggccgtcgc cgtctgcgtg aagattcgcc accggttacc gctcgacggg atgttgccaa
      901 ctccgtgggc gacgacggcc gcgtggccct gggcaggcga cggcgcggcc ggtccgagcg
      961 cctgggcgag gacgccgacg atcgagccgc ccgcgagcag ggctccgacg agcagcgaaa
     1021 ggatcgccat cgcgggccgg cgacggatcc agttgagcag tcttccgggc agggacgcgg
     1081 gattggtccc ggcgccacgt ggtgcgggcg tggtcgtcat gcggtctccc tgacgcctgg
     1141 tggtccgatt cgggaccgaa ccagaagtgc gggcgggtgg cttggggagc acactccgtg
     1201 gcgaatccaa tggaacgcac cgaattccat ctcccggtgc atgcgccaac cgtacgcgcg
     1261 cgcggtgacg acgtcaacgt cgccgttcga gccggaaacg cggagagacc gccgtcgatt
     1321 cgacggcggt ctctcgattc cagtcatgca accggaacag gcagtatcta ggcggcgccg
     1381 aagttcttgc cggcgacatc ccagttgatg acattccaga tcgcctcgag gtacgccggg
     1441 cgcttgttct ggtacttgag gtagtaggcg tgctcccaga cgtcgatacc gatgaccggg
     1501 gtctggccgt cgctgagggg gctgtcctgg ttcgcggtgc tgacgacctt gagggcgcca
     1561 ccgtcgacga caagccatgt ccagccggac ccgaagcggg cggcgccagc ggcgttgacc
     1621 tgctccttga gcgagtcgac gctaccgaag gtggattcga tcgcggcggc gagagcgccg
     1681 gtcggggcgg tgctgccgcc cggagtgagg agttcccaga agaaggtgtg attgacatcg
     1741 ccaccggcgt tgttgcggac cgcggtgcgc ttcgcttccg gaacctggct caggtccttc
     1801 acgaggtcga tcgagctgac gctgtcgaga tccgtcccgg caaccgcgtt gttgaggttg
     1861 gtgacgtagg tctggtggtg gcgatcgtgg tggatctcca tcgtctgcgc gtcaatgtgc
     1921 ggctcgagcg cgtcaaaagc atatgggaga ggagcgagtt cgcgtgccat cagagtcaca
     1981 tccttcattg aaagtgatcg agtgtcgaag cacgccgatc gaacggtctg ctcgtccggc
     2041 gacgtatctt ctgccaacgc gcccattgtc gcaagagcgc tgccagatgt aaagagtttt
     2101 cgaagaaatc tcttgtaaaa ctccgcggaa aaggggggat gcgtggccga ttctcatgga
     2161 ggcccatgag gcgtcggggg attcgtgatg gcaggagcgg caatcggggt tgacacggca
     2221 aaacgcgcag tttaggctgg tctcgttatg gacgatcaac gcacgaatcc accagtcgaa
     2281 gagcgaacga aatccacggc tcccgcaggg aacgcgtgga atagctcgtt ttattactac
     2341 tggtacttta ccaacccccc ggctgcgctg gcggggcgaa agggctgcat gcagtaacca
     2401 acgccaccag gaccaaaagc gcaagaccag gggggagccg agacaaatgt ctcggttttt
     2461 tgtttgtccg gaattcccgt cccagttcgc acatct
//
LOCUS       DASTMD010000454         5375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_221838, whole genome shotgun sequence.
ACCESSION   DASTMD010000454 DASTMD010000000
VERSION     DASTMD010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221838"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VFQ54_06590"
     CDS             complement(<1..748)
                     /locus_tag="VFQ54_06590"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU0164697.1"
                     /translation="MTLSYLQRNRIAFAEYVRMTMRRVYIYRVNVVMGMLLTGITIYL
                     LTILWKAAYGDRTSVGGVSIDQMLVYLTIANLQLRFLAPQLDQDIEERIREGQIGFDL
                     NRPLSYPFQLVAGAAGQMVGLIPMIVLALPIAFVFGELRAPTSVSNGIAYAVSLALAW
                     IVAVELNMVIGLVSFWTLEMTGFRMMYNLVSNFATGALIPLWFMPDLLRIVIQVLPFQ
                     SIAYIPVSIYIGNPATGHIWSALLIQVVWMG"
     gene            complement(745..1743)
                     /locus_tag="VFQ54_06595"
     CDS             complement(745..1743)
                     /locus_tag="VFQ54_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0164698.1"
                     /translation="MPIIEVEHLVKEFHRPKRFEGPFGGVRTLFTREFETKRAVSDVS
                     FSIDEGELVGYLGSNGAGKSTTIKMLSGILVPTDGKVEVVGRVPWQDREQNALAIGVV
                     FGQRTQLWWDLPLADSLKLIGKLYRVEPDRYKRNLATMVDLLGLDPFMDTPVRQLSLG
                     QRMRGDLAAAMLYEPRILYLDEPTVGLDVVAKERTRQFVEQVNRELGTTIILTTHDLA
                     DVERLCKRIIFIDKGTVLYDGSVAELKYRYAPYRMLDVTLAPNGGEPIVLDIPGVEIE
                     SQEPTRLLIRFDPNVIAVTDLIAAVISRHPVSDISIIEPDLEGVVREIVEDGEIRA"
     gene            complement(2242..3075)
                     /locus_tag="VFQ54_06600"
     CDS             complement(2242..3075)
                     /locus_tag="VFQ54_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0164699.1"
                     /translation="MAFVTVGKENSTDIEIFYEDHGSGQPVVLIHGYPLNGRSWEKQS
                     AVLLQAGYRVITYDRRGFGLSSQPTVGYDYDTFASDLRILLETLDLAEVGLFGFSMGT
                     GEVARYIGTYGSERISKAGLLAPIPPFLLLTDDNPEGVPQAVFDGILQTAQTDRYAWF
                     TAFYENFYNLDQNLGTRISEEAVRASWNVAVGASWFASSAVVPTWLTDFRADLPKIDV
                     PTLILQGTDDRILPFASSGQRLPALIADHEFVPIEGAPHGLLWTHGDQVSEALIEFLA
                     K"
     gene            complement(3217..4089)
                     /locus_tag="VFQ54_06605"
     CDS             complement(3217..4089)
                     /locus_tag="VFQ54_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0164700.1"
                     /translation="MTDTIKTIANHVENRRGAITKLVAAGAGAAILATGQQMLASAQA
                     TPGASPVASPVANLEPIVVLVHGAFADGASWGGVIAELQSTGIPALATANPLRGIKAD
                     ATYLDSVLAGIPNPVILVGHSYGGAVITNITATNVIGLVYVAAFVPDENEVALQVLGS
                     HTATELGTALLPASVPASEAEVEVIIDPTKFASIFCADLPATQAAVLAVSQRAVMAAA
                     FSEPSGVPAWKTIPTWYALANQDMALGIDVETFYAERTGGTIVKIDGSHAVAVSQPAA
                     VAEVIKQALVALKP"
     gene            4352..4822
                     /locus_tag="VFQ54_06610"
     CDS             4352..4822
                     /locus_tag="VFQ54_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEU0164701.1"
                     /translation="MPAPELTTDALWNLLAVRSDGVLATIKRDGRPQLSNISYHYDPE
                     TRILRTQAATFRAKYNNMLRDPRVSIHVSSEDFRLWVVAEGTAEVGEPVQTHDDAVGR
                     ESVAYIRARYPELSDAELEEHLAEYPVIGRALIAIKVERIYGGNGSIARGISAS"
     gene            complement(4829..>5375)
                     /locus_tag="VFQ54_06615"
     CDS             complement(4829..>5375)
                     /locus_tag="VFQ54_06615"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU0164702.1"
                     /translation="LEWLMAHPDSAVVDVGTGSGAIAISIAANLPETSNSMVVASDPS
                     IEALRIAIQNRTLHGDKGIVRRILMVQQSLLSETTGHVDLVLANLPYLTPAQIAENPD
                     LEAEPRLALDGGADGLDLVRDLIADLPRVLAADGAAGFELDPAQTATVAQLLRDTFPG
                     ATIRTIDDLAGLARHVVMTRA"
BASE COUNT          983 a         1604 c         1835 g          953 t
ORIGIN      
        1 gtcccatcca gacgacctgg atcagcagtg cgctccagat gtgcccggtt gccggattcc
       61 cgatgtagat cgagaccggg atataggcga tcgactggaa cggcaggacc tggatcacga
      121 tccggagaag atccggcatg aaccagagtg gaatcagggc cccggtcgcg aagttggaga
      181 ccaggttgta catcattcgg aagccggtca tctccagcgt ccagaagctg acgagcccga
      241 tcaccatgtt gagttcgacc gcgacgatcc acgccagggc gaggctgacg gcgtaggcga
      301 tcccgttcga gaccgatgtt ggcgccctca gctcgccgaa gacgaacgcg atcggcagcg
      361 cgaggacgat catcgggatc aggccgacca tctgaccggc ggccccggcg acgagttgaa
      421 acggatagct cagcgggcgg ttgagatcga acccgatctg cccttcccgg atccgctcct
      481 cgatgtcctg atcgagctga ggggcgagga agcgaagctg gagattggcg atcgtgaggt
      541 agacgagcat ctggtcgatc gagacgccgc cgaccgaggt gcgatcgccg taggccgcct
      601 tccacaggat cgtcagcagg tagatcgtga tgccggtgag gagcatcccc atcacgacgt
      661 tgacgcgata gatgtagaca cgtcgcatcg tcatgcggac gtattccgcg aaggcgatcc
      721 ggttgcgctg gaggtagctc agcgtcatgc gcggatctcg ccgtcttcga cgatctcgcg
      781 gaccacgcct tcgaggtcag gctcgatgat cgagatgtcc gagaccggat ggcgcgagat
      841 taccgccgcg atcagatcgg tcaccgcgat cacattcggg tcgaagcgga tgagcagtcg
      901 cgtcggttcc tgcgactcga tctcgacacc cgggatatcg agcacgatcg gctcgccgcc
      961 gttcggcgcg agcgtcacgt caagcatgcg gtagggtgca tagcggtact tgagttcggc
     1021 aaccgagccg tcgtagagca cggttccctt gtcgatgaag atgatccgct tgcagaggcg
     1081 ctcgacatcg gcgagatcgt gggtcgtcag gatgatcgtc gtgcccagtt cgcgattgac
     1141 ctgttcgacg aactggcgcg ttcgctcctt cgccacgaca tcgagcccga ccgtcggctc
     1201 gtccaggtag agaatccgcg gctcgtagag catcgccgcc gcgagatcgc cacgcatgcg
     1261 ctgcccgagg ctcagctgac ggacgggcgt gtccatgaac gggtcgagtc cgaggagatc
     1321 gaccatcgtc gccaggttgc gcttgtagcg atcgggttcg acccggtaga gcttgccgat
     1381 caacttcagg gagtcggcga gggggagatc ccaccagagc tgggtgcgct ggccgaagac
     1441 gacgccgatt gcaagcgcgt tctgttcccg gtcctgccag ggaacgcgtc ccacgacctc
     1501 gaccttcccg tcagtcggga cgaggatgcc ggagagcatc ttgatcgtgg tcgacttccc
     1561 ggcgccgttg gagccgagat agccgacgag ctcgccctcg tcgatgctga acgagacgtc
     1621 actgacggcg cgcttcgttt caaattcgcg tgtgaagagc gtgcggacgc cgccaaaggg
     1681 tccttcaaac cgcttcggtc gatggaactc cttgacgagg tgttcgactt cgatgatggg
     1741 cacgtatgga tttcccggag gtgaggagaa acaggacggc agcaccgcgg attgccgccc
     1801 gggcgcgagt ctgggagata tacccgcgaa cctgtgaatg tgtcaatgac tctcgggtgc
     1861 gccggcggga ggcgggacat tactccatcc aaagaccgac atccgcagct gtgaaacacg
     1921 tcgatcgcag tctttggatt ccgcagtccc gtccggacgc tgcactactg aacagtcgac
     1981 catgactcgc gtttttctga cccgttcgtc gaccaccggt cgttcgtagg gcgggatcgc
     2041 cgctgccaga tcgcagatct ggacggcctg tcccgcctct cgctgggtcg cgaggcgttc
     2101 tccgccacct gccgcccccc gccgactcgc aaggcgcttc tgcccaacgt cggggcgcga
     2161 ccacatgggt cgcggggacg ggatgggaaa caccggctct cctgagcggg agagccggtg
     2221 tcctgtttcc gtggattcga attacttggc gaggaactcg atgagcgctt cgctcacctg
     2281 atcgccgtgc gtccagagca ggccgtgcgg tgcgccctcg atcggcacga actcgtgatc
     2341 ggcgatcagc gccggcaggc gctggccgga cgaggcgaac ggcaggatcc gatcgtcggt
     2401 gccctggagg atcagcgtcg gcacgtcgat cttcgggaga tcggcgcgga agtcggtgag
     2461 ccacgtcggg acgacggccg aggatgcgaa ccaggaagcg ccgacggcga cattccagct
     2521 cgcgcgaacc gcttcttcgc tgatccgcgt gcccaggttc tggtcgaggt tgtagaagtt
     2581 ctcgtagaag gcggtgaacc acgcatagcg atcggtctgg gcggtctgca ggatgccgtc
     2641 gaagacagcc tgcggcacac cctccggatt gtcgtccgtc agtaggagga atggcgggat
     2701 cggggcgagg agaccagcct tgctgatccg ttcgctgcca taggtgccga tgtagcgggc
     2761 gacttcgccg gttcccatcg agaagccgaa gagaccgacc tccgcgagat cgagggtctc
     2821 gaggaggatc ctgagatcgg aagcgaaggt gtcgtagtca tatcccaccg tcggctggct
     2881 ggagagcccg aagccgcgac ggtcgtaggt gatgacgcga tagcccgcct ggaggaggac
     2941 ggcggattgc ttctcccacg agcggccgtt cagcgggtag ccgtggatga gcacgacggg
     3001 ctgcccgctg ccgtgatcct catagaagat ctcgatatcg gtcgagtttt ccttgccaac
     3061 ggttacaaaa gccatctgcg aaatcctttc ggctgttcct gtgtctccgg ggagggcatc
     3121 cgatcctccc cggaagatcg tgacgggaga tgatgtggaa ccacgacctg caaggcgtat
     3181 gccgcacgcg cgatcgtctg cgccactctt tcaggtctac ggcttgaggg cgacgagcgc
     3241 ctgcttgatg acttcggcga ccgcggccgg ctgcgagacg gcaaccgcgt gggagccgtc
     3301 gatcttgacg atcgtgccac cggtgcgctc ggcgtagaac gtctcgacgt cgatgccgag
     3361 cgccatgtcc tggttggcga gggcgtacca ggtcgggatc gtcttccagg ccggcacgcc
     3421 tgacggctcg gagaaggcgg cggccatcac cgcgcgctgc gagaccgcga gcaccgcggc
     3481 ctgcgtggcc ggaaggtcgg cgcagaagat gctcgcgaac ttggtcgggt cgatgatgac
     3541 ttcgacttca gcttcagacg ccgggacgga ggccggcagg agcgcggtgc cgagttcggt
     3601 cgcggtgtgg gaaccgagga cttggagcgc cacttcgttc tcgtccggca cgaatgcggc
     3661 gacgtagacg agaccgatca cgttggttgc cgtgatgttc gtgatgacag cgccaccgta
     3721 ggagtgtcca acgaggatga ccggattcgg aatcccggcg agcacgctgt cgagatacgt
     3781 ggcgtcggcc ttgatgccgc gcagcggatt cgccgttgcg agagccggga tgcccgtcga
     3841 ctgaagttcg gcgatcacgc cgccccagct ggcgccatcg gcgaaggcgc cgtgcacgag
     3901 gacgacgatc ggctcgagat tggcgaccgg tgaagcgacg ggagatgcgc ccggcgtcgc
     3961 ctgggcgctg gcgagcatct gctgaccggt ggcgagaatg gcggcaccgg cgccggctgc
     4021 tacaagcttc gtgatcgcac cgcggcgatt ttcgacgtga ttggcgatgg tcttgatggt
     4081 gtcggtcatg tcagggcctt tcgtgatggt cctggcccgg cctcgtggcc acgcagcagg
     4141 ggatgctgga aacaggggag tcgatgaagt cgggtgcgca actgtcctcg tgccaacctc
     4201 cattcgtggc aatgtggtgt attcggttgt tttgcaggga cccatagaac cataccatgc
     4261 ggcgtccccg gttcgacgat gtcgcgcgac gcgatgtccg cgccatagaa tgcagggcag
     4321 cgaatccgcc gtaaccagaa ggacatcttc gatgcccgct cccgaactca cgaccgacgc
     4381 actctggaat cttctcgccg tacgaagtga cggcgtgctg gcgacgatca agcgcgacgg
     4441 tcgaccgcaa ctctcgaata tctcgtatca ctacgatccg gagacccgga ttctacgcac
     4501 gcaggcggcg accttccgcg ccaagtacaa caacatgctg cgcgatcccc gtgtcagcat
     4561 ccatgtctcg agcgaggact ttcggctctg ggtggtggcc gaaggaacgg ccgaagtcgg
     4621 cgagccggtc cagacccacg atgatgcggt cgggcgggaa tcggtcgcct acatccgggc
     4681 gcggtacccg gagttgagcg atgccgagct cgaggaacac ctggcggagt atcccgtgat
     4741 cgggcgtgcg ctgatcgcga tcaaggtcga gcgcatctac ggtgggaacg gatcgatcgc
     4801 gaggggaatc tccgcgtcgt agtcgcgttc aggcgcgcgt catcacgacg tgccgcgcga
     4861 gcccggcgag atcgtcgatc gtccggatcg ttgccccggg gaacgtgtcg cggagaagct
     4921 gcgcgacggt cgccgtttgc gcgggatcga gctcgaaccc cgctgctccg tccgcggcaa
     4981 gaacgcgagg gagatcggcg atcaggtcgc gaacgagatc gagaccgtcg gcaccaccat
     5041 ccagggcgag acggggctcc gcctcgagat cgggattctc cgcgatctgt gcgggtgtca
     5101 ggtaaggcag gtttgcgagg acgagatcga cgtgaccggt cgtctccgac aggagcgatt
     5161 gctgaaccat gaggatgcgg cgcacgatcc ccttgtcccc gtggagcgtg cgattctgga
     5221 tcgcgatccg gagtgcctcg atcgagggat cggaggcgac gaccatcgag ttggacgttt
     5281 cggggagatt cgccgcgatg ctgattgcga tcgcgccgga accggtcccg acgtcgacga
     5341 cggcggaatc cggatgcgcc atgagccatt ccagc
//
LOCUS       DASTMD010000455         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1560701, whole genome shotgun sequence.
ACCESSION   DASTMD010000455 DASTMD010000000
VERSION     DASTMD010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560701"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..683
                     /locus_tag="VFQ54_06620"
     CDS             51..683
                     /locus_tag="VFQ54_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU0164703.1"
                     /translation="MAKGLSIETIVQAGFEVLGETGLDGMTMRIIAARLGVKAAAIYW
                     HLKSKQDLLDEMATQQWREIIGEIAGFPESTTSAEGLAAFAHIVRATLLKYRDGARLF
                     AGTFLTDSSLLAEQRERMSWMIDAGYTVKALIQAYALIYNFTVGFTIEEQAIRQAADD
                     RYSLETRAERLGPDVDPIVLAAGVEVFSDSGQRFEELIALLVQTAESLRS"
     gene            complement(778..1191)
                     /locus_tag="VFQ54_06625"
     CDS             complement(778..1191)
                     /locus_tag="VFQ54_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164704.1"
                     /translation="MQRSIRFPLRTILLADAAISGLSGLVMIVLASPLADLLELPTAL
                     LRVSGLILIPYVGALIYVATRKEIPVRGVWAVIATNLLWTAGSVVTLFSGEIDANAGG
                     ILVIVVQAVAVALIAELEVLALHGHQQSGRTSLAA"
     gene            complement(1489..1863)
                     /locus_tag="VFQ54_06630"
     CDS             complement(1489..1863)
                     /locus_tag="VFQ54_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164705.1"
                     /translation="MTDTTIDITTEQLVNDYIAIWNEEDAASRGELIARTWELDGEYV
                     DPLLQGSGHDGIEAMTAGVQSTYPGHKFVRIGAIDQHHDRIRFGWQLLTPEREPLVIG
                     TDYGVVSADGRLKAITGFFEPD"
     gene            2034..>2753
                     /locus_tag="VFQ54_06635"
     CDS             2034..>2753
                     /locus_tag="VFQ54_06635"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0164706.1"
                     /translation="MERTVGDVLREWRRRRHLSQLDLATEAEISPRHLSFVETGRSNP
                     SRDLLIHLADQLKVPLRERNALLTAGGYAPVYQQKPLDDPTLAAAKEAVQVMLRCHEP
                     NPAVAIDRHWNLIAANRTIGPLLVDVAPELLEPPINILRLAMHPGGLASRIVNVQEFG
                     NLLVERLRHEIEISGDPELVDLAKELVAFQRGQPGVVTSSAPPPNVFGIAVPFQLRTD
                     EGILSFFNMTTVFGTATEVTLS"
BASE COUNT          484 a          837 c          908 g          524 t
ORIGIN      
        1 taattgaacg ttgttaaggt gtcaagacgc cgcaaggggg gagggcacgc gtggcgaagg
       61 gactctcgat cgagacgatc gtgcaggcgg ggttcgaggt gctcggggag accggtctcg
      121 acgggatgac aatgcggatc atcgccgccc ggctgggagt gaaggcggct gcgatctact
      181 ggcatctcaa gagcaaacag gatttgctcg acgagatggc aacgcagcag tggcgcgaga
      241 tcatcgggga aatcgccggg ttccccgagt ccacgacgtc tgccgaagga ctggccgcgt
      301 tcgcgcacat cgtgcgggcg acgctcctga aataccgcga cggcgccagg ctcttcgcgg
      361 ggacgtttct caccgattcc tctctcctcg cggagcagcg ggagcggatg tcgtggatga
      421 tcgatgccgg ctacacggtg aaggccctca tccaggccta tgcgctcatc tacaacttca
      481 ccgttggttt cacgatcgag gagcaggcga ttcgccaggc ggcggacgat cgctattcgc
      541 tcgagacccg ggccgaacgt ctcggtcccg acgtcgaccc catcgtgctc gccgccggcg
      601 tggaggtatt cagcgactcc gggcagcggt tcgaggagtt gattgcgctt ctcgtgcaga
      661 cagcggaatc gcttcgctcc tgatccgggc cggcgcccgg acgcgaagag acgccgctga
      721 attccatcag cgacgtcgcg tcgatgaacc ggactggtgg agtgaccagt atcccggtca
      781 tgctgcgagt gaggttcgtc cagattgctg atggccgtgg agcgccagga cttccagttc
      841 ggcgatcagc gcgacggcga cagcctggac gacgatcacg aggatgcctc cggcgttcgc
      901 gtcgatctca ccgctgaaga gcgtgacaac gctgcccgct gtccacagca ggttcgtcgc
      961 gatcacggcc cagacgccgc ggacaggaat ctccttgcgt gttgccacgt agatcagggc
     1021 gccgacgtac gggatcagga tcagtcctga aacgcgaagc agcgccgtcg gcagctcgag
     1081 cagatcggcg agtgggctcg ccagaacgat catcaccagg ccgctgagcc cgctgatcgc
     1141 ggcgtccgcc agcaggatgg tgcggagcgg gaatcggatg ctccgttgca tggtgaattc
     1201 ctccttgact ccgggctgcg agccggcggc gcacgatcca cccgctcgca gatgcattcg
     1261 atcggatccg gcgttgtccg gcgcgtccgg tcgttcgagg ggtgggaccg ccgctgccgg
     1321 atcggagatc cggacggcct gtcccacctc tcgttcttcg cggtgcgatg atccctggtg
     1381 gtctgtcacc ggtgttttgc cggttggcat cgttatagcc aacgtcatat gcggcttcgc
     1441 cgcatgggcc acacgggtgt tcagactgga gacgtgcgtg acgcaccact agtccggctc
     1501 gaagaagccg gtgatcgcct tcagtctgcc gtcggcggaa acgacgccat agtcggtgcc
     1561 gatcaccagt ggctcacgct ctggcgtgag cagctgccag ccgaagcgaa tccggtcgtg
     1621 atgctggtcg atcgcgccga tgcgaacgaa cttgtggccc gggtaggtgc tctggacgcc
     1681 ggcggtcatc gcctcgatcc cgtcatgacc cgagccctgg agcaggggat cgacgtattc
     1741 gccatcgagc tcccatgtcc tggcgatcag ttcgccgcgg gatgcggcgt cctcttcatt
     1801 ccagatcgcg atgtagtcgt tgacgagctg ctcggtcgtg atatcgatcg tcgtgtcggt
     1861 catcgtctgt gcctcctctg gcagcttggg ctccggtttg gccgaccacc ctggcggctc
     1921 ctggtgcctg gaattcagtc tgccggggcg ggaaatcgaa tccaattacc tcggaggtaa
     1981 gtgaaaattc ggggcacttg cgtatgattg aggacatgaa tgcaatgaac gcgatggagc
     2041 gcacagttgg agatgtcctg cgagagtggc ggcgacggcg ccacctgagc cagctcgatc
     2101 tggcgacgga agccgagatc tcgccacgtc atctcagctt cgtggagacg gggcgctcga
     2161 atccgagtcg cgacctgctg attcatctcg cggaccagct caaggttccc ctgcgtgagc
     2221 gcaacgccct gctgaccgcg ggcggatatg cgcccgtcta ccagcagaaa ccgctcgacg
     2281 acccgacgct cgccgccgcg aaggaagcgg tgcaggtcat gctccgttgt cacgagccga
     2341 atcccgccgt cgcgatcgat cgtcactgga atctcattgc cgcgaaccgg acgatcggtc
     2401 cgctgctggt cgatgtcgca ccggagttgc tggagccgcc gatcaacatt ctccgattgg
     2461 ccatgcatcc gggcggattg gcgtcgcgta tcgtgaatgt gcaggaattc ggaaatctcc
     2521 tggtggagcg gttgcgtcac gagatcgaga tcagcggcga ccccgagctc gtcgatctcg
     2581 cgaaggaact cgtcgcgttc cagcgcggtc agccgggcgt ggtaacgtca tcggccccgc
     2641 cgccgaatgt gttcgggatc gcggtcccat tccaattgcg gactgatgag ggtattctca
     2701 gcttcttcaa catgaccacg gtcttcggaa cggcgacgga agtcaccctg tcc
//
LOCUS       DASTMD010000456         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2097875, whole genome shotgun sequence.
ACCESSION   DASTMD010000456 DASTMD010000000
VERSION     DASTMD010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097875"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFQ54_06640"
     CDS             complement(<1..360)
                     /locus_tag="VFQ54_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164707.1"
                     /translation="MAAANAKGSAIQPIEIFRVGSHTAMNGDVLDVTPQMLEQVAAGY
                     DPARHEAPLVIGHPKTDAPAMGWVKSLHVENDGLFATVDQLEPEFAELVRQGRFKKIS
                     GSFWTPGTTGNPVPGSLR"
     gene            complement(360..1307)
                     /locus_tag="VFQ54_06645"
     CDS             complement(360..1307)
                     /locus_tag="VFQ54_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEU0164708.1"
                     /translation="MSSMTVAQARVIDPVLTSVAQGYKHADRVGSVLFPRVDVTARGG
                     KILEFGREGFRLYASRRAPGAPTLHVPFAYEGKDFNLAQDALNSSVPRELLDDARRVP
                     GVDMGTRAVSTCMFALTLTLEAEQAKLATTPGNFDLNHRLILTGEDRWSAPDSNPVAD
                     IKDAKEKVRRSVGVDPNRMVVSKPIFNALKMHPKIVDRFKYTSSDSITAAMLANLFDL
                     DMIAIGKAAALEGPDETAQFVDVWGNFATLAYVPAAGAGYEEPSFGYTYTLKGHPFVE
                     QPYWDGDKKSWIYGVTYERRPVLTGMLAGFLFQSVIEEA"
BASE COUNT          286 a          453 c          498 g          301 t
ORIGIN      
        1 gcgtagagat ccgggaaccg gattgcccgt tgtgcctggc gtccagaagg agccgctgat
       61 cttcttgaac cgaccttgtc ggacgagttc ggcgaactcc ggttcgagct gatcgaccgt
      121 ggcgaaaagc ccgtcgttct cgacatggag cgacttgacc cagcccatcg caggcgcgtc
      181 ggttttgggg tggccgatga cgagaggagc ttcgtgccgg gcgggatcgt agcctgccgc
      241 gacctgttcc agcatctgcg gcgtcacgtc gagcacatcg ccgttcatcg cggtgtggga
      301 gccgacgcgg aagatttcga taggctggat cgcgctgccc ttcgcgttcg cggcggccat
      361 cacgcttcct cgatgacgga ctgaaacagg aagccggcga gcatgccggt cagcaccggc
      421 cgtcgctcgt aagtcacgcc atagatccac gatttcttgt cgccgtccca atagggctgc
      481 tcgacgaacg gatgtccctt cagcgtgtag gtgtatccga aggaaggctc ctcatagccg
      541 gctcccgcag ccggtacata ggcgagcgtc gcgaagttcc cccatacgtc gacgaactgg
      601 gccgtctcgt cggggccctc cagagcggca gccttgccga tggcaatcat gtcgagatcg
      661 aacaagttag ccagcatcgc tgccgtgatg ctgtcagacg acgtgtattt gaaacgatcg
      721 acgatcttcg gatgcatctt gagtgcattg aatatgggtt tggaaacaac catacggttc
      781 ggatcgacgc ccacggacct gcgaaccttt tccttcgcgt ccttgatatc ggcgacgggg
      841 ttactgtcag gggcggacca tctgtcttca cccgtgagga tcagcctgtg attgaggtcg
      901 aagttgccgg gggtggtcgc cagcttcgcc tgctcggctt cgagcgtcag cgtcagcgcg
      961 aacatgcagg tgctgacggc ccgcgtgccc atgtccacgc ccggaaccct gcgcgcatcg
     1021 tcgagcagtt cgcgcgggac actagaattc agcgcgtcct gagcgaggtt gaagtccttg
     1081 ccttcataag cgaacgggac gtgcagcgta ggcgcccccg gtgcccggcg cgacgcgtag
     1141 agtcggaaac cctcgcgacc gaattcgagg atttttccgc cgcgcgcggt gacatccaca
     1201 cgagggaaca gcacggaccc gacgcggtcg gcgtgcttgt agccttgcgc gacactggtc
     1261 agaaccggat cgatgacgcg agcctgcgcg acggtcatac tggacatagt cactcctgaa
     1321 aatgatggaa aatgccggaa ggcgtttgga agcccgtagg cgggcgttcg atgctctcgg
     1381 ctattgagct agcgggcata gtccgcgacc cccgtcagag gggcgtcaga atcccggcga
     1441 atgcgcatgc gatctgcatt gggcaggcgg tagccaaggg ttcgctcgac ggtgcggtat
     1501 gaaacgttga gctcgagggc tatccgactc gtggtctg
//
LOCUS       DASTMD010000457         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2543524, whole genome shotgun sequence.
ACCESSION   DASTMD010000457 DASTMD010000000
VERSION     DASTMD010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543524"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="VFQ54_06650"
     CDS             complement(<1..1141)
                     /locus_tag="VFQ54_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164709.1"
                     /translation="MTTPISPERAVFDLPSLGDAAISPDGSRIAYVRSHVDRETAKAG
                     RQIWIVNADGTGKRQLTYIGSNGEPTWSPDGTALAYVSRRDGDLPFAISLMRFDGGEP
                     AVLTTHAAAPSSLVWSDDGKTIAYQKPVDPANPNETPRDPKAPPAVRVVKRIDYKQDG
                     IGYVNDVRNQIFLLDVETGERRQLTRDLVDHTKPDWSPDGASLAVIVSNRNGQRTQLG
                     VVNVATGTVTLHGDYDGEVGLPSWSPDGKTIFVQGDARGMPHHGIFTLDVASGAFTSL
                     IEDPEFYLDGTILSTTVPIWLDNGNVLVSAQHHGTSGLLTVAPATGEIGPVGFWAATH
                     VGLSVSADGTTIVQTSNDLDGAVSIVRIDRTTGERTTLFDEAEEFF"
     gene            complement(1138..>2977)
                     /locus_tag="VFQ54_06655"
     CDS             complement(1138..>2977)
                     /locus_tag="VFQ54_06655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0164710.1"
                     /translation="VDRETGKSESQIWVCNIDGTERRRLTWIGTRNADPVWSPDGSQL
                     AYVAARDGDHPYAIVILGFDGGDSRVLTRHASSPSSLAWSPDGSTIAYTKAVDPDNPE
                     ETPRDPKATPAVRLVKRMDFRQDGLGFINNIRSQIFLLDVATSERHQLTHTPVDHTRP
                     TWSPDGKALAIGLPNRNGLRGQIGILDVASGDLRASTPYDGAVALFAWSPDGTRLLFD
                     GAYPYSPHHDYSLVDVATATVTPVTTDVHFLPDVGYQSGGNAGAPVWLDEKTALVHGL
                     SKGRSGIWKLDLATGNTTEVAIWNEMHSSFAVSADNSTVVQAVSSIDGVIGLMKLDVA
                     SGESSLLFNEAADVFAATPLAAWELVSIERAGFTVEGWLLKPANFDERKTYPIILDVH
                     GGPHNAYGYGINALAEMYASNGYFVLLPNPRGSGTYSRAFAEAVIGDWGGGDWADLEA
                     ILDHVLERPYIDSDRTGIYGYSYGGYMTAWVLGHTDRFRTIICGAPVFDFESTFGQSD
                     IGHVRATGEPPWEAREYLTDRSPATHIQNAVTPTLIIQGEADERCPVEQSEQLFMSLM
                     MLGVETEFIRYPGQSHLFINSGDPAYRLDFFARTLAWFNRFLEEDR"
BASE COUNT          500 a          898 c         1040 g          539 t
ORIGIN      
        1 cgaagaactc ctccgcctcg tcgaagagcg tcgtgcgctc cccggtcgtg cgatcgatgc
       61 ggacgatact gactgcgccg tcgagatcgt tcgacgtctg gacgatcgtc gtcccgtcgg
      121 cggagacact gagacccacg tgcgtggcgg cccagaagcc gacggggccg atctcaccag
      181 tcgccggtgc caccgtcagc agaccactcg tcccgtgatg ctgcgcgctg acgagcacgt
      241 tcccgttgtc gagccagatc ggcaccgtgg tcgagaggat cgtgccgtcg agatagaact
      301 cggggtcctc gatcagactc gtgaaagccc cgctcgccac gtcgagggtg aagatgccgt
      361 gatgcggcat cccgcgcgcg tcgccctgga cgaagatcgt cttgccgtct ggcgaccagg
      421 acggaagacc gacttcgccg tcgtaatcgc cgtggagcgt gacggtgcca gtcgctacat
      481 tgacgacgcc cagttgggtc ctctgtccat tgcggttgga gacgatcacc gcgagcgatg
      541 cgccgtctgg cgaccagtcc ggcttcgtgt ggtcgacgag gtcgcgcgtc agttgtcgcc
      601 gctcgcccgt ctcgacatcg agcaggaaga tctggttgcg gacgtcattg acgtacccga
      661 tgccgtcctg cttgtagtcg atgcgcttga cgacgcggac cgcgggcggt gccttcgggt
      721 cgcgcggcgt ctcgttcggg ttcgcgggat cgaccggctt ctggtaggcg atcgtcttgc
      781 catcgtccga ccagaccagg ctcgatggcg ccgcggcgtg cgtggtgagg acggctggct
      841 cgccgccgtc gaagcgcatc agggagatcg cgaagggaag gtcaccatcg cggcgactca
      901 cgtaggccaa tgccgtgcca tctggcgacc aggtcggttc gccgttgctg ccgatgtagg
      961 tcagctggcg cttcccggtg ccatcggcat tgacgatcca gatctgacgc cccgccttcg
     1021 cagtttcgcg gtcgacgtgc gagcggacgt aggcgatacg cgagccgtct ggcgagatgg
     1081 ccgcatcgcc gagcgatggg agatcgaaga cggctcgttc tggagagatt ggcgtggtca
     1141 tcggtcctcc tcgagaaagc ggttgaacca ggcgagtgtc cgcgcgaaga agtcgagccg
     1201 gtaggccggg tcaccactgt tgatgaagag atgggattgg ccggggtagc ggatgaactc
     1261 ggtctcgacc ccgagcatca tcagggacat gaagagttgt tccgactgtt cgacggggca
     1321 acgctcgtcc gcctcaccct ggatgatcag ggtcggggtc accgcgttct gaatgtgcgt
     1381 ggctggcgag cgatcggtca ggtattcgcg cgcttcccac ggtggttccc cggtggcgcg
     1441 cacgtggccg atatcggact gcccgaaggt cgattcgaaa tcgaagacgg gcgcgccgca
     1501 gatgatggtc cggaagcgat cggtatgacc gaggacccag gccgtcatgt agccgccgta
     1561 cgagtagccg tagatgccgg ttcggtcgct gtcgatgtag ggccgttcga ggacgtgatc
     1621 gaggatcgcc tcgaggtcgg cccagtctcc gcctccccag tcgccgatca cggcttcggc
     1681 gaaggcgcgg ctgtaagtgc cggaaccgcg cggattcgga agcagcacga agtagccgtt
     1741 cgatgcatac atctcggcca gcgcgttgat cccgtagccg taggcgttat gcggtccgcc
     1801 gtggacatcg aggatgatcg gataggtctt cctctcgtcg aagttggcgg gtttgagcag
     1861 ccatccttcg acggtgaagc cggcgcgctc gatcgagacg agctcccacg ccgcgagcgg
     1921 cgtcgcggcg aagacgtcgg ccgcctcgtt gaagagcaac gacgattcgc cggacgcgac
     1981 atcgagtttc atcaggccga tcacgccgtc gatgctggaa acggcctgca cgacggtcga
     2041 gttgtcggcg gagacggcga aactggagtg catctcgttc cagatcgcga cctcggtggt
     2101 gttaccggtc gcgaggtcga gtttccagat gccggaacga cccttcgaga gaccatgaac
     2161 gagcgcggtc ttttcgtcca accagacggg cgccccggcg ttgccgccgg actggtagcc
     2221 gacatccggc aggaaatgga cgtcggtcgt gaccggcgtg acggtcgcgg tggcgacgtc
     2281 gaccagcgaa taatcgtgat ggggactgta gggataggca ccgtcgaaga ggagtcgcgt
     2341 gccatccggg gaccaggcga agagtgcaac ggcgccgtcg tatggcgtcg atgcacgcag
     2401 gtcaccgctg gcaacgtcga ggatgccgat ctgtccgcgc aggccgttcc ggttcgggag
     2461 gccgatcgcc agcgccttcc cgtccggcga ccacgtcggc cgcgtgtgat cgacgggcgt
     2521 gtgcgtcagc tgatggcgct cgctggtcgc cacgtcgagc aggaagatct ggctgcggat
     2581 gttgttgatg aagccgagac cgtcctggcg aaagtccatc cgcttcacga ggcgaacggc
     2641 gggtgtcgcc ttcggatcgc gcggcgtttc ctccggattg tcgggatcaa ccgccttcgt
     2701 gtaggcaatc gtcgagccgt cgggagacca ggcgagactc gatggcgacg acgcgtgacg
     2761 ggtcaggacg cgggaatcac cgccgtcgaa accaaggatc acgatcgcgt aggggtggtc
     2821 gccgtcgcgc gcggcgacat aggcgagctg cgagccgtcc ggtgaccaga ccggatcggc
     2881 gttgcgcgtg ccgatccagg tcaggcgtcg tctttcagtg ccgtcgatgt tgcagaccca
     2941 gatctggctt tcggacttcc cggtctcccg gtcgacc
//
LOCUS       DASTMD010000458         1894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_490321, whole genome shotgun sequence.
ACCESSION   DASTMD010000458 DASTMD010000000
VERSION     DASTMD010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490321"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..897)
                     /locus_tag="VFQ54_06660"
     CDS             complement(190..897)
                     /locus_tag="VFQ54_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0164711.1"
                     /translation="MGRILVVEDERELADLVRRELEHAGHQVEIVGDGPGGVEAVDRF
                     GPDLILLDVMLPGFDGLEVTRRVRANHATPILMLTARSTELDKVVGLELGADDYLTKP
                     FSMRELLARVTAVLRRVELIRARSVQREASARLEFPSLTIDVDAHDVTSAGAYVGLTA
                     TEFNLLKLLASNPNRVFSREYLLEEVWGGEAAVFDRTVDSHIQRLRKKLGGPGTPGDY
                     IETVWGVGYRFSPRVHR"
     gene            complement(946..1128)
                     /locus_tag="VFQ54_06665"
     CDS             complement(946..1128)
                     /locus_tag="VFQ54_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164712.1"
                     /translation="MIVAIVLLIVFGLLFAAMAIAPAAIAGVEPQPKLIVRPQAPIGI
                     DVSRTTGAVEHEPQAA"
     gene            complement(1278..>1894)
                     /locus_tag="VFQ54_06670"
     CDS             complement(1278..>1894)
                     /locus_tag="VFQ54_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU0164713.1"
                     /translation="DNGATTRIQSARSPVAVGAAAPFSSPPGVSETVESGPTRWDRAY
                     ALGGKVARRTGRFRGILWRLTLILFIGNLLLGTVLLVRGGPSALVERFLAVAPNTTTT
                     VNIDELNLRVAPDLDAPVITVLDQGDKVRVTGLSETSGPYQFWPVDVTIDGSTVTGWV
                     WGDGLAPNEWTGRLGWVQGIVDQGRDIDHQVNDWTGTVTSLWPF"
BASE COUNT          368 a          629 c          571 g          326 t
ORIGIN      
        1 tgctcagggt cacgacgagg agatgggaga cggtcagctt ggtgcgaagt gatctccgga
       61 tgttctgccg aaagccgcgt acccggggca cgaccgaggg ccgaatgctg atcgcagacc
      121 tcggctcggc ggggcgcggc cggctggacc gcgactcggt agcgatcgag tcgagatccg
      181 ggagcgattt caccggtgta cccgaggcga gaagcggtag ccgacacccc agacagtctc
      241 gatgtagtcg ccgggtgtgc caggaccacc gagtttcttg cgcagccgct ggatgtggga
      301 atcgaccgtt cgatcgaaca cggccgcctc tccaccccag acctcttcga gcaggtactc
      361 gcgactgaag acgcggttcg gattgcttgc gagcaacttc agcagattga attccgtcgc
      421 ggtgagtccg acgtacgccc ccgcgctcgt cacgtcgtgg gcatcgacat cgatcgtcag
      481 cgatggaaac tcgagccggg cggaagcttc ccgctgaacc gacctcgcac ggatgagctc
      541 gacgcgccgt aaaacggcgg taacgcgcgc cagcaactcc cgcatcgaga acggcttcgt
      601 gagatagtcg tcggccccga gttccagtcc gacgaccttg tcgagctcgg tggagcgcgc
      661 ggtcagcatc aggatcggcg tggcatggtt ggcgcggacg cggcgcgtga cctcgagacc
      721 gtcgaagccc gggagcatga cgtcgagcag aatgagatcg ggcccgaagc ggtcgactgc
      781 ctcgactccg cctggaccat ctcccacgat ttcaacctgg tgaccggcgt gttcgagttc
      841 gcgccgaacg agatccgcga gttctcgctc gtcctcgacc accaatattc tgcccaacga
      901 ctccccctga catcccgctc gcccctggcg agccggatcg tgacattatg cggcctgcgg
      961 ttcgtgctcc accgcacctg tggttcgcga cacgtcgatc ccgatcggcg cctgcgggcg
     1021 aacgatcagt ttcggctgcg gctcgacccc ggcgatcgcg gccggagcaa tcgccatcgc
     1081 cgcaaacaac aggccaaaga cgatcaagag aacaatagca acaatcatgg tgtttattcc
     1141 tcgttgaata ccggagaacc ggcaacggct tccctcacac catcagtttg attgttgtct
     1201 gttgcagcat cactacccat tcatgacaaa accgcgaaga aatgtggaga aactgtgacg
     1261 gacgagcaca gcgcgatcta gaacggccag agcgacgtta ccgtgcccgt ccaatcgttc
     1321 acctgatgat cgatatcacg cccctggtcg acgataccct gtacccaacc caggcgtccc
     1381 gtccattcgt tcggcgccag gccgtcgccc catacccatc ccgtcaccgt cgacccgtcg
     1441 atcgtgacgt cgaccggcca gaactgatag ggccccgatg tctcggagag accggtcacg
     1501 cggaccttgt ctccttgatc gagaaccgtg atcaccggag catcgaggtc tggtgcgacc
     1561 cgcaggttga gttcgtcgat attgacggtc gtcgtggtat tcggagcgac ggcgaggaag
     1621 cgttcgacga gcgcactcgg tccgccgcgc acgagcagca cggtccccag cagcaggttc
     1681 ccgatgaaga gaatcaacgt caatcgccag aggattcccc ggaaacgccc tgtccgcctc
     1741 gccaccttcc cgcccagcgc gtaggcccgg tcccaccgcg tcggcccgct ttcgacagtc
     1801 tcgctcaccc cgggcggcga cgaaaacggc gccgcggccc caacggcaac cggcgatcga
     1861 gcactctgga ttcgcgtcgt cgccccgttg tcga
//
LOCUS       DASTMD010000459         3099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_400697, whole genome shotgun sequence.
ACCESSION   DASTMD010000459 DASTMD010000000
VERSION     DASTMD010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400697"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..888
                     /locus_tag="VFQ54_06675"
     CDS             391..888
                     /locus_tag="VFQ54_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEU0164714.1"
                     /translation="MSPEDARPSTSAPPTSLSMESPPERSRGRSRHAIGRGLRIHLFV
                     FAVVAVDLLVLNLITNRDTIWAVWPIGVWAVLVAAHLGANLVKPAFFGVHLLGGGAIC
                     LGLGIINIFHGRDDASAAGFWVIWPILAWLVSLVIHLPFAFDLLASTRPSRRAPATVA
                     STPER"
     gene            1171..>3099
                     /locus_tag="VFQ54_06680"
     CDS             1171..>3099
                     /locus_tag="VFQ54_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0164715.1"
                     /translation="MATPESHDSPLGRLYTSFTQGAVSRRAFVRRATAMGVSAATATF
                     LANAGAQAASGKPGRNGFAFTNQDATPAASPVASPAAAVGSASTVGAPDAGTAKQTRG
                     AGSEFRALIWQAPTIAAPHSALSDKDYMAAAPVIEPVLQYLPDGTIVPNLVEQVPTVE
                     NGLLKEDLTEATFVFLKGLTWSDKTPVTANDLVFTWKWVTTAANQATSATTWGTIKNI
                     VAKDDRTAVVTLNKPSATWFEPFSGNYNGNLYPAHIFNNDPTNKNDAFLLAPIGTGPY
                     VLSKFSPNDSAEYSINENYRDATKPYFKSIALKGGGDATSAARAVLQTGDYDYAWNVQ
                     VAPDVLTSIAKDSKYAKVYSSPGTTIESLNFNFSDPNKDVDGQKSQKDTPHPLFSQLA
                     VRQALNASVDRQLITDKLYQPPVEHPTANLLAGLPSFDSPNTTWEFDIDKANKILDDA
                     GWTKDGDTRSKDGVKFSFNYWTSITDVRQKTQAIVKADWQKLGASIELDQVDGGVFFS
                     GDASAEQNFYHMYWDLDMWTNGPYSALPFSYMNRWYAGPNGENIAQKENGWARDNTQR
                     YNNPDYDALYEKLIDTLDPNEAAQLLIKMNDLLIADVVEIPIVVRAFSTYALANRVRN
                     DNLANGPSFVLPFWNIANW"
BASE COUNT          590 a         1029 c          940 g          540 t
ORIGIN      
        1 acgacgatgt gatgggggtt cggcgtgatc gtgatcggat gatccggtcc cggaatgagt
       61 cgcggcttgt cgctggtggt ggatcgctcg gtcatggaaa tccctcttct cttggatgga
      121 tagcgatgca gatgtccgtc cgggtgcgcc gtctccatca tccggcgctt tgacgagtgt
      181 cgaaccggac cgatcgaccg tcaactggaa cgatgggcag ccccccttca tgcgcagcga
      241 ggggaacagg ttgcctcagc ggtgggggat agctgcaaaa tcgggtcgca cggataggcg
      301 gcgacgatgg cggttgggta tgctggtcgc aaggatcgcc caggcaccac acgcatccgt
      361 tcaccgtggc gatcaggaag aggaccagtg atgtctcccg aggacgcgag gccgtcgacc
      421 tccgcacccc caaccagtct ctcgatggaa tcgcctcccg aacgaagccg gggccggagt
      481 cgccacgcga tcgggcgggg actgcgtatc catctcttcg tcttcgctgt cgttgcggtc
      541 gatctcctcg tgctcaatct catcacgaac cgggatacga tctgggcagt ctggccgatc
      601 ggcgtctggg ccgtgctcgt cgccgcgcat ctcggtgcga atctggtcaa gccggcgttt
      661 ttcggcgtcc atctgctcgg cggcggggcg atctgcctcg gactcggcat catcaacatc
      721 tttcacgggc gcgacgatgc ctcggccgcc ggcttctggg tgatctggcc gattctcgcc
      781 tggctcgtct ccctggtgat ccaccttccg ttcgccttcg atctgctcgc ctccacccgg
      841 ccgtcccgtc gcgccccggc gaccgtggca tcaacgccgg agcgatagtc cgcgcacagc
      901 cactccctat ctggccgatc tccggttgcg attgctgcat ttccccgtac aatcccttgt
      961 gaggaacctg tgccaggtat ccgccgcacc tgcagccgcg cgagcctctg cacaggaatc
     1021 gatcgcccct gaatgggcgg tccgggactt aatcgatcga tcatgaactc atcggatcgt
     1081 cggcgcacga tgatcttgcc ggacgcatgc agatgtccgc cgtgattcga tgagcgcgac
     1141 gcgacaagac atcggtacag agagaagatc atggccaccc cggaatcgca cgattcacca
     1201 cttggacgtc tttacacatc cttcacgcag ggagcagtct cgcgtcgcgc tttcgtccgg
     1261 cgcgccaccg cgatgggcgt gagcgccgca acggcgacct tccttgcgaa tgccggagcc
     1321 caggccgcca gcggcaagcc cggccgcaac ggttttgcct tcacgaacca ggatgcgacc
     1381 ccggcggctt cgcccgtcgc ctcccccgcg gcggccgttg gctccgcgag tacggtcggg
     1441 gcgccggatg ccggaaccgc gaagcagacg cgcggcgcgg gcagcgagtt ccgcgcgctg
     1501 atctggcagg cgccgacgat cgccgcgccg catagcgcgt tgagcgacaa ggactacatg
     1561 gcggcggccc cggtgatcga gccggtgctg caatacctgc ccgatggtac gatcgtgccg
     1621 aatctcgtcg agcaggtgcc gacggtcgag aacgggctcc tcaaggagga cctcaccgag
     1681 gcgaccttcg tcttcctcaa gggtctgacc tggagcgaca agacgccggt caccgcgaac
     1741 gatctcgtct tcacctggaa gtgggtgacg accgccgcca accaggcgac ctcggccacg
     1801 acctggggca cgatcaagaa catcgtcgcc aaggacgatc gcaccgccgt cgtcaccctc
     1861 aacaagccat cggcgacctg gttcgagccg ttcagcggta actacaacgg caacctttac
     1921 ccggcgcaca tcttcaacaa cgacccgacc aacaagaacg acgccttcct gctggcgccg
     1981 atcggcaccg gtccctacgt gctctcgaag ttctccccga acgattctgc cgagtacagc
     2041 atcaacgaga actatcgcga tgcgaccaag ccgtacttca agagcatcgc gctcaagggc
     2101 ggcggcgacg ccacctcggc ggcgcgcgcc gtcctccaga ccggcgatta cgactacgcc
     2161 tggaatgtcc aggtcgcgcc ggatgtgctg acgtcgatcg ccaaggacag caagtacgcc
     2221 aaggtctact cctcgccggg cacgacgatc gagagcctga acttcaactt ctccgatccg
     2281 aacaaggacg tcgacggcca gaagtcccag aaggacacgc cgcacccgct cttcagtcag
     2341 cttgcggttc gccaggcgct caacgcctcg gtcgatcgcc agttgatcac ggacaagctc
     2401 taccagccgc cggtcgagca cccgacggcg aacctgctcg ccgggctccc ctcgttcgat
     2461 tccccgaaca cgacgtggga gttcgacatc gacaaggcga acaagatcct cgacgacgct
     2521 ggctggacga aggacggcga cacgcgcagc aaggacggcg tgaagttcag cttcaactac
     2581 tggacatcga tcacggatgt ccggcagaag acgcaggcga tcgtgaaggc ggactggcag
     2641 aagctcggcg cctcgatcga gctcgaccag gtcgacggcg gcgtcttctt cagtggggat
     2701 gcctcggcgg aacagaactt ctatcacatg tactgggacc tcgacatgtg gacgaacggc
     2761 ccctactccg ccctgccgtt ctcgtacatg aatcgctggt acgccggccc gaacggcgag
     2821 aacatcgcgc agaaggaaaa cgggtgggcg cgcgacaaca cgcagcgcta caacaacccg
     2881 gactacgatg ctctctatga gaagctgatc gatacgctcg acccgaatga agcggcgcag
     2941 ctgctgatca agatgaacga cctgctgatc gcggacgtcg tcgagattcc gatcgtggtc
     3001 cgcgccttca gcacctatgc actggcgaat cgcgtccgca acgacaatct tgcgaacggt
     3061 ccgagtttcg tgcttccgtt ctggaacatc gcgaactgg
//
LOCUS       DASTMD010000460         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2721460, whole genome shotgun sequence.
ACCESSION   DASTMD010000460 DASTMD010000000
VERSION     DASTMD010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721460"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1157
                     /locus_tag="VFQ54_06685"
     CDS             147..1157
                     /locus_tag="VFQ54_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU0164716.1"
                     /translation="MSDVKDRPAVGQQQATLPLLGHTGDAPRGGVPAFAHPAEQEVAR
                     LLTFFGLRWAYEPTTFALKRHPDGRIAEAFSPDFFLPDLNLYLELTTMRQKLVTRKNR
                     KARLLRELFPNVQLRLIYRRDYLRMMECYRGEMRRFDGADISDVVRSEHEIAEEIDRI
                     ALRLIARLQRTGGGTGGSVVLLAVSRSSRHFQMQLANRLEAHGVSVTRLDLSIATTEG
                     IGSQPRIRFARTSLQVLRETPVVLVETLVSSGLTLGHAQRWLTARGVGPMETIALFAR
                     TNCRIGNAVLDHVSFEAPNDVLAGYGLQLRAAYADLPYVGKVHAGSGSSLDPFFFSTA
                     PE"
     gene            1310..>2081
                     /locus_tag="VFQ54_06690"
     CDS             1310..>2081
                     /locus_tag="VFQ54_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164717.1"
                     /translation="MAISNLTQRLEEQSARYFDRPGFSSPSFIDADHIALLDDRNGVP
                     QISVLDLTDGSIEPATTFGERVLTLLASSASGIIVFGMDEGGNEYQQIWAIAPGGSPR
                     RLTTSDTSRHEPVSLSRDGKYVYFRSNARSVGEFDIERIDLVSGERSILLENAGQPMD
                     FVPSADGSKLYVESAIGNLAAILFELDTVDGTIRNLTPGPEGATIFSIAAAGDDALWV
                     STNLDRDFGALVKLGLTTLESELVLARYWDVEAVSPSPD"
BASE COUNT          377 a          664 c          654 g          386 t
ORIGIN      
        1 gccattgttc gtgttgaggg attgtgccac tattcgctta ccccgcaaga tcaatcgagt
       61 tgtacatggc gcagtccagc gaatcgctgc cttcacgatg gggatcgtat ggatcgctac
      121 ccgatgctga aagaacaggc gtcccgatgt cagacgtgaa ggaccggccg gcggtcggcc
      181 agcaacaggc gaccctgcca ttgctcggcc ataccggcga cgcgccgcga ggcggggttc
      241 cggccttcgc gcatccggcg gagcaggaag ttgcgcgtct gttgaccttc tttggtctgc
      301 gatgggcgta cgagccgacg accttcgcgc tgaagcgcca tccggacgga cggatcgccg
      361 aggcgttctc accagacttc ttcctgcccg atctcaacct ctacctcgaa ctgacgacga
      421 tgcggcagaa actggtgacg cggaagaatc gcaaggccag gctgttgcga gagctcttcc
      481 cgaatgtcca gcttcgcctg atctatcgcc gcgactacct ccggatgatg gagtgctacc
      541 gcggcgagat gcgccggttc gacggtgccg atatctccga tgtcgtgcga agcgagcatg
      601 agatcgccga ggagatcgac cggatcgcgc tacgcctgat cgcgcgcctg cagcgaaccg
      661 gaggcggaac gggcggctct gtggtcctgc tcgcggtcag tcgcagttcg cgtcacttcc
      721 agatgcagct cgcgaatcgg ctggaggcgc atggcgtcag tgtcacgcgg ctcgatctct
      781 cgatcgcgac gaccgagggg atcggatccc aaccccggat caggttcgcc agaacatcgc
      841 tccaggtact tcgggagacg ccggtcgtgc ttgtcgagac gcttgtgagc agcggactca
      901 cactgggtca cgcgcagcgg tggctgaccg cgaggggggt cggaccgatg gaaacaatcg
      961 ccctcttcgc ccggacgaac tgccggatcg ggaacgccgt cctcgaccac gtctcgttcg
     1021 aggcgccaaa tgacgtcctg gccgggtatg gattgcagct gcgcgccgcc tatgccgatc
     1081 tgccctatgt cgggaaggtt catgcgggct ccggatccag cctcgacccc ttcttcttct
     1141 cgaccgctcc cgaatagatt cggaatccgg ccggtaggaa gggacgattc gtcttgcggg
     1201 acggggcaag ttgtgcgtca atcggatgcc ttcagggcgc cgaaccatcc cgccatgccg
     1261 gaccgcaatt cgtagcggag ccgccggcag cacaaggagc acacccacca tggcaatctc
     1321 caatctcacg caacgcctgg aggaacagtc cgcccgctac ttcgatcgcc ccggattctc
     1381 atcgccgtcc ttcatcgacg ccgatcacat cgcgctgctc gacgatcgca acggtgtgcc
     1441 tcagatctcg gttctcgatc tcacggatgg ctcgatcgaa ccggcaacga cgttcggcga
     1501 gcgggtcttg accctcctcg catcgtcggc ctcgggaatc atcgttttcg gcatggatga
     1561 aggcggcaac gagtatcagc aaatctgggc gatcgcgccg ggcggaagcc cgaggcggct
     1621 gacgaccagc gatacgtcgc gacacgagcc ggtctcgctc tccagggatg gcaagtacgt
     1681 ctacttccgc tcgaacgccc gatcggtcgg cgagttcgac atcgagcgga tcgatcttgt
     1741 ctcgggcgag cgctcgatcc tgctcgagaa cgcgggccag ccgatggact tcgtgccgag
     1801 tgcggacgga tcgaagctct acgtcgagag cgcaattggc aacctggctg ccatcctctt
     1861 cgagctcgat acggtcgacg gaacgattcg gaatctgacg ccgggacccg aaggcgcgac
     1921 gatcttcagc attgccgcgg ccggtgacga cgcactctgg gtctcgacga acctggaccg
     1981 cgacttcggt gcactcgtca agctgggcct gacgacactc gagtccgaac tcgtcctcgc
     2041 ccggtattgg gatgtcgagg ctgtctcgcc ttcgccggac g
//
LOCUS       DASTMD010000461         4506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_131091, whole genome shotgun sequence.
ACCESSION   DASTMD010000461 DASTMD010000000
VERSION     DASTMD010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131091"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VFQ54_06695"
     CDS             complement(<1..831)
                     /locus_tag="VFQ54_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164718.1"
                     /translation="MRDGIGAALIRALVVCVTVVGSVAGFGAAIVAAAPGPAASPVTT
                     TITPTSTPDFAAADIPVSGLTLSNVQVTPATYSFLDVDGTVTVTFDLTNPSGTDSSTP
                     VNLQVNSYNGGLYGSTATVLPQGVVIPPDGTQSFTMTFVLSQEYWEVLANVVASDISL
                     FVGDGTKNLFSVYYQLGMPAATAIGFSCAATLPAGTTAGTTIPARFTLQNNTQAVPLG
                     RAPASQWASITYQGITSTAPGAIASGQQFEADYTYSVTADDIAACAGCATTTLSATTA
                     S"
     gene            complement(987..2936)
                     /locus_tag="VFQ54_06700"
     CDS             complement(987..2936)
                     /locus_tag="VFQ54_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU0164719.1"
                     /translation="MPDDPRNRPLPDPSEPTPAPGADDYLRRTIRRARRRNPASPDQE
                     TFRQRPEQRYAAYRNVNDPGQTAPRRRDQLPPRAALDAGATLPPSGLDAEFDVAERSQ
                     IATAKSRRKRSRRPRLLRKPWAYATLGIPATFLLVFLVIAAPLAYKSYNAYREIKVGP
                     VEHVDSAKVAVVNAAGTPELAVAPTKPPSDDWTGTDRINILLLGVDNAEGGVSRTDTI
                     IVVNIDPVAKKASMISIPRDLKVVIPGYGIDKINAAYALGEYNKVQGGGAGLLIRTIE
                     ANLGIPIDHFAQIDFAGFEKMIDTVGGITIDNPYPIKDDEYPAANYQYERIYFPAGWQ
                     HLNGAQALEYARTRHQDGDGRRSERQQQVLLALRDKAINLNMISKVPTLIDQFAQFTR
                     TDISAGDALKLARLGASMNSSAITQYSLMPAMSDEQLPGEPYYLVADWSETGAILSQF
                     TGTTVNPPGAALANPNFNLPILVENGTNNQGLAGRVGSVLEENGFTNVQVALTTDGEH
                     QTTELVDNGGNLGTSATLANLIGVGADRIDVENMNLAGTATPGATPGLSTPSATPNPA
                     DPNQFAIVITLGDDAPDPGDSQWQIQDYQDQVGDDDTGDGPIATEAPTSNDSSDPGAN
                     PTGIPTEVPTEDPYSLPSDGTSGGQ"
     gene            3359..3952
                     /gene="dcd"
                     /locus_tag="VFQ54_06705"
     CDS             3359..3952
                     /gene="dcd"
                     /locus_tag="VFQ54_06705"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEU0164720.1"
                     /translation="MVLSDRDILIALEQGRIVIDPYPDMEIQLGSVSVDFRLGSTFMV
                     FEHSRHSYIDPRRPQSIGDAMRTIEVTEDEPFIMQPGDFALASTMERLELADDLLGRL
                     EGRSSIARLGITVHSTAAVFEPGWIGTATMELSNLGRMAVALYPGMLICAFSFETVSS
                     PVMTPYRKKLGNKYAGQQTPQASQLSEETRRATEASR"
     gene            complement(4044..>4506)
                     /locus_tag="VFQ54_06710"
     CDS             complement(4044..>4506)
                     /locus_tag="VFQ54_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164721.1"
                     /translation="LFVPLVLALIGATGLYEPFGDLERISTPYWWQIRISGAFILLVA
                     QSLAALIVFGFDHTGVAVVRNLVAFTGMAACLAVVIDPRQVWVVLLPVWILVFLLDDS
                     RYPDTWPNVLWAWPLSSMQSPGAWVQAVAWSVAGIVTLAGVGAKLRRGLQE"
BASE COUNT          775 a         1406 c         1446 g          879 t
ORIGIN      
        1 cgacgccgtc gtggccgaaa gcgtagtcgt cgcgcaaccc gcgcacgcgg cgatatcatc
       61 cgccgtcacg ctgtacgtgt aatccgcctc gaactgctga ccggacgcaa tcgcccccgg
      121 cgcggtcgac gtgattccct ggtacgtaat gctcgcccac tgcgatgccg gcgcccggcc
      181 gagcggcacc gcctgcgtat tgttctgaag ggtgaaccgg gcgggaatcg tcgtcccggc
      241 ggtggtcccc gctggcaacg tcgccgcgca ggagaaaccg atcgccgtgg cagccggcat
      301 cccgagctga tagtagaccg aaaacagatt cttcgtgcca tcgcccacga aaaggctgat
      361 atccgaggcc accacattgg caaggacctc ccagtactcc tggctgagca cgaaggtcat
      421 cgtgaagctc tgggttccgt ctggcggaat caccacgcct tgcggcaaga ccgttgccgt
      481 tgaaccgtag agtcccccgt tgtaggaatt gacctggaga ttgaccggcg tactcgagtc
      541 ggtcccactg ggattggtca ggtcgaaggt cacggtcaca gtgccgtcga cgtcgaggaa
      601 ggaatatgtg gcaggtgtca cctgaacatt gctcagcgtg agccccgaga ccgggatgtc
      661 tgccgccgcg aagtccgggg tcgatgtcgg cgtgatcgtc gtggtgaccg gagatgccgc
      721 cggcccaggc gccgccgcca cgattgccgc gccgaatccc gccacacttc ccacaacggt
      781 gacgcacacc accagcgccc ttatcagggc ggcaccgatt ccgtcacgca tgtcttgttg
      841 gtccttgtcg ttcccaccac accacattcg cccgtaggca ttgccccgga tgctagcagg
      901 tcaaccagat gcgcggatat aggacgttcg gatatcccgg ttctggtgcg ggccgcgagg
      961 atgtcgaaga accgcgatcg tgggcgctac tgccccccgc tcgtgccgtc ggacgggagc
     1021 gaatacggat cctcggtcgg gacctcagtc gggatcccgg tcggattggc gccgggatcc
     1081 gacgagtcat tcgaggttgg cgcctcggtc gcgatcgggc cgtcaccggt atcgtcgtcc
     1141 ccgacctgat cctggtaatc ctggatctgc cattgtgaat cgccaggatc cggcgcgtca
     1201 tcgccgagcg tgatcacgat cgcgaactga ttcggatcgg cgggattcgg ggtggccgac
     1261 ggcgtggaaa ggccgggcgt tgctcccggt gtcgcggtgc cggcgagatt catgttctcg
     1321 acatcgatcc ggtccgcgcc gaccccgatc aggttcgcga gggtggccga ggtcccgagg
     1381 ttgccgccgt tgtcgaccag ttccgtcgtc tggtgctcgc catccgtcgt cagtgccacc
     1441 tggacattcg tgaagccatt ttcctcgagc acgctgccga cgcgtcccgc cagtccctgg
     1501 ttgttcgtgc cgttttcgac caggatcggg agattgaagt tcggattcgc gagggcggct
     1561 cccggcggat tgaccgtcgt ccccgtgaac tgggagagga tcgcgccggt ctcggaccag
     1621 tcggctacca gatagtaggg ttcaccggga agctgttcgt cgctcatcgc cggcatcaat
     1681 gagtactggg tgatcgccga cgagttcatg gacgcgccga gccgcgccag cttgagcgcg
     1741 tcaccggcgc tgatgtctgt ccgggtgaac tgcgcgaact gatcgatcag ggtcggcacc
     1801 ttggagatca tgttgagatt gatcgccttg tcgcggagtg cgaggagtac ctgctgctgg
     1861 cgttccgacc ggcggccatc gccgtcctgg tggcgggtgc gggcgtattc gagcgcctgc
     1921 gccccgttca ggtgctgcca gccggccggg aagtagatcc gctcgtattg gtagttcgcg
     1981 gccggatact cgtcgtcctt gatcggatag ggattgtcga tcgtgattcc cccgacggta
     2041 tcgatcatct tctcgaagcc ggcgaagtcg atctgggcaa agtgatcgat cgggataccg
     2101 agattcgcct cgatcgtccg gatcagcaga ccggcccctc ccccctgcac cttgttgtac
     2161 tcaccgagcg cgtaggcggc attgatcttg tcgataccgt agccgggaat gacgaccttc
     2221 agatcgcgcg ggatcgagat catcgaggcc ttcttcgcca cggggtcgat gttgacgacg
     2281 atgatcgtgt cggtccgcga aacgccgccc tccgcattgt cgacgccaag caggaggatg
     2341 ttgatgcgat cggtccccgt ccagtcgtcg gacggtggct tggttggcgc gaccgccagc
     2401 tccggtgtcc ccgccgcatt gacgacggcg accttcgccg aatcgacgtg ttcgaccgga
     2461 ccgaccttga tctcgcggta ggcgttgtag ctcttgtagg caagcggtgc cgcgatcacg
     2521 aggaaaacga gcaggaaggt agcggggatg ccaagcgtcg cgtatgccca cggctttcgg
     2581 aggagccgcg gccggcgact gcgcttcctg cgcgatttcg ccgtcgcgat ctggctgcgc
     2641 tcggcaacgt cgaactcggc atcgagaccg gatggcggca gggttgcacc agcgtcgagc
     2701 gcggcacggg gcggaagctg atcccgacgt cgtggtgccg tttgccccgg atcgttgacg
     2761 tttcgatagg ccgcgtaccg ttgttccggt cgttggcgga atgtttcctg atcgggactg
     2821 gcgggattcc ggcgccgggc acgccggatc gtacgacgga gatagtcgtc cgcgcccggt
     2881 gccggggtcg gctccgaggg atcgggcagt ggccggtttc gaggatcgtc tggcatgcgt
     2941 cagtctatcg ggtactcgtg agcctggctg ctcggcggac ggtctggatt gctgttgggt
     3001 gtgatggtcg ccgtgactcg cggcatgagc ttcgctgccg cgagtctacc acgcgtgtac
     3061 gttccatcgg tccgggcgga tccgccttcg gcgggtcgcc ggcggctcgc gttgtccgtc
     3121 ccgggatggc cttccgcagg actcaactgc gctttatgtc acgccgagtt cgcggattga
     3181 cgtgtgcaga tgggaccaat tgaccgatga cagatgtgta cgtacactta tcctgatctg
     3241 atgggtgtcg tgtgtctcgt tgcaccgggc gcggcttccc cttccggcgg gcgaacagtc
     3301 gcaccgccgc cgtggcgtct gcagggttca ccctcgcgcc gtgacgacgt gaggcaccat
     3361 ggttctttcc gatcgcgaca tcctgattgc gctggaacaa ggccggatcg tgatcgatcc
     3421 ctatccggac atggagatcc agcttggctc cgtctccgtc gatttccggc tcggttcgac
     3481 cttcatggtc ttcgagcaca gccgtcacag ctacatcgat ccccgccgcc cccaatcgat
     3541 cggcgatgcg atgcgcacga tcgaggtcac cgaggacgag ccgttcatca tgcagccggg
     3601 ggatttcgcc ctcgcctcga ccatggaacg actcgaactc gcggacgatc tcctcggccg
     3661 gctcgaggga cgatcgagca tcgcccgtct cgggatcacg gtgcattcga cagcggcggt
     3721 cttcgaaccc ggctggatcg gcacggcgac gatggaactg agcaatctcg gccggatggc
     3781 ggtcgcgctc tacccgggca tgctaatctg cgccttctcc ttcgagacgg tctcgtcccc
     3841 ggtgatgacg ccctatcgca agaagctcgg caacaagtac gccgggcaac agacgccgca
     3901 ggcgagccag ctctccgagg aaacgcgccg cgcgaccgag gcgtcgcggt aggtcccgcc
     3961 aacgtccagc cttctctctg ttaacgatga cgacctcttg tgtgctcaga ggtcatctat
     4021 ttgtaacgtg gcctataatg cttctattcc tgaagacctc ttctcaactt ggctccaact
     4081 cctgcgagcg tgacgatacc cgcgacgctc caagcaacag cctgtaccca tgcacctgga
     4141 gattgcattg aggagagcgg ccacgcccac agcacattcg gccatgtgtc cggatacctc
     4201 gaatcgtcga gaaggaagac aagaatccag accgggagaa ggacgaccca gacttgtcgt
     4261 ggatcaatca cgaccgcaag gcaggcagcc attcccgtga acgcgaccag attgcgtacc
     4321 accgccactc cggtgtgatc gaatccgaag acgatcagcg cggcgaggct ctgcgcgacg
     4381 agcaaaatga aggcacctga aatgcgaatc tgccaccaat atggagtgga tatgcgctcg
     4441 aggtcgccga acggttcgta gagccccgtt gctccaatca gcgccaacac gagaggcacg
     4501 aataag
//
LOCUS       DASTMD010000462         5217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_935679, whole genome shotgun sequence.
ACCESSION   DASTMD010000462 DASTMD010000000
VERSION     DASTMD010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935679"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..724
                     /locus_tag="VFQ54_06715"
     CDS             125..724
                     /locus_tag="VFQ54_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU0164722.1"
                     /translation="MSTTFTVDFFCSLDGYGMAKGWPGYWGKEGPELIEDRVRTFAQD
                     QVLVFGATTFRQFSKFITMFDEPYYDSLNELPKIVFSSTLEEPLGWQNSTVLNEDAVT
                     AIERLKRTTDVPMRSHGSISLNRALLAAGLVDRIEVMMFPAISGQAGRAALFHDGPEF
                     DLELIESTVLDGRTHKLVYAPHLHGEIPEGAGPKLRTAD"
     gene            complement(953..1534)
                     /locus_tag="VFQ54_06720"
     CDS             complement(953..1534)
                     /locus_tag="VFQ54_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEU0164723.1"
                     /translation="MVRLILFVTFAVPLFILAVMGYVYYEAHNDQARRVDAIVILGAA
                     QYNGTPSPVLQARLNQALALWNDGYAPRVVVTGGKMPGDAYTEAESEANFLMDNGVPS
                     SAILYENKGRDTWQSMQGVAAVLKGTGVKKLLLVSDGFHLARSKLMATELGFTAYGSP
                     AKNSPIKSWSGDEFHYVIRETGGIIDFLPKFIF"
     gene            complement(1778..3733)
                     /locus_tag="VFQ54_06725"
     CDS             complement(1778..3733)
                     /locus_tag="VFQ54_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0164724.1"
                     /translation="MTMSDVAWIPTEDYLRDNRLLRLIEKTGSGDYQGLLARVAEDPT
                     WYWEQALADLDLAWQKPWSQIMDLSRGKAWPRWFPDAEFNYVASALDRHADGTLADQP
                     AVIWEGDDESTRTLTFAELGETTDRFAHVLRGLGVGKGDKVGIFLPMIPEVVAATLAC
                     GKLGAIYVPLFSGFGEESIVSRIGDSDAKVMIVADGFLRRGKFVPMKAVVDKALARLP
                     LIEHVVVVRRTGAEIDWTEGRDVWWDEAMATADSSRITEPTAADDPYMILYTSGTTGR
                     PKGAVHIHAGFPIKATHDLAYSFDLHQGDVLFWMTDMGWMMGPWLIAGGLTLGATILL
                     FEGTPDYPTSDRLWRIVEKHRVTTLGVAPTAIRALIQYGEEIVKATDRSSLRILGSTG
                     ETWNPDPWRWFLDVVGDGRCPIINYSGGTETSGGIISSLPIFPIKPCSFTGPIPGMVA
                     DVVVVDAGGAPVRDGVGELVIREPWVGMTNGFWRDPDRYEETYWSRLPGVWVHGDFAE
                     IDADGFWYIRGRSDDTLKVAGKRIGPAEVESAAVAHGTVREAAAIGVPHPVKGDVAIV
                     FCVLRSDQTATDELAEQIKRFIGNQLGAALRPDRVVFVNDLPKTRNAKIMRRVIRAAW
                     LGLPAGDTSALENPDAVESIRLVAVES"
     gene            3885..4787
                     /locus_tag="VFQ54_06730"
     CDS             3885..4787
                     /locus_tag="VFQ54_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164725.1"
                     /translation="MPWRQFWKREKQAESGQEAKSAPPATEPTPSVPASNLPPHMQRI
                     VGERERPANRPPLNPAEKRARLQRQRLAILFDVDQGELAEQDDNPWSSRISLLTDALE
                     TVNVDRARLLRIAPSPYAPVPATPIADLTISETQPIAISFQIGPERFAYEEDLDWAER
                     GHQLALPELHQKTGCVDAIIPQDFPNDLREPLAAHLSHSLDVFAADLRDRRLDDEPLP
                     ESPTLADIARPCPTCGGWTDWKGRCAACAARAADELQLNREQRRLLDERAREAEERHR
                     LRDRLPLARKRLADVDAEIAALDE"
     gene            4780..>5217
                     /locus_tag="VFQ54_06735"
     CDS             4780..>5217
                     /locus_tag="VFQ54_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164726.1"
                     /translation="MNDALTHIARGLVDRHTFGVVMLVAIAAIGWRNRSSFRGMLADD
                     DAFWRLTARLFVGSFVATAVWIAIFDDWIQLFSEPYRRTRDLSVDRVVADPIPTELRA
                     ITLILLAITIIATAAIVARHIGGYGLQIGALLLSITFWLILFIF"
BASE COUNT          921 a         1793 c         1632 g          871 t
ORIGIN      
        1 gaaccagcac gccattcaac cgccatcaag aagttcagga agagatgtcg gatcgggccg
       61 gtcgtgttcg tagaaggagt gaagccgccc acgagaggtg gcgcgaagaa agaggaggaa
      121 caccatgagc acgaccttca ccgtcgactt cttctgcagc ctcgatggct acggcatggc
      181 caagggttgg cccggatact ggggcaagga gggcccggag ctgatcgagg accgggtacg
      241 caccttcgca caggaccagg tgctcgtctt cggcgcgacg acgttccggc agttcagcaa
      301 gttcatcacc atgttcgacg agccctacta cgacagcctg aacgaactgc cgaagatcgt
      361 gttctccagc acgctcgaag agccgctcgg atggcagaac tcgacggttc tcaacgagga
      421 cgcggtgacc gcgatcgaac gcctgaagcg caccacggac gttccgatgc gctcgcacgg
      481 cagcatctcg ctgaaccggg ctctgctcgc cgccggcctc gtcgaccgga tcgaggtcat
      541 gatgttcccg gccatctcgg ggcaggccgg gcgagcggca ctcttccacg acgggcccga
      601 gttcgacctg gagctcatcg agtcgacggt gctcgacggt cggacgcaca agctcgtgta
      661 cgccccgcac ctgcacggag agatccccga aggcgccggc ccgaagctgc gaaccgccga
      721 ctagtcctcg gcaaggcaca cggtcttgcc cgacaaaccc cgatcaggtt caacgtggcg
      781 acggtccgtg atttcgcatc cccgcaagag tgaggacgct tgcctacagc tgcgaatcgc
      841 gcgtcgggca cccgttcgtt cgtagggcgg gaccgccgct gccggaccgc aggtccggac
      901 ggcccgtccc gcccgggatt cgcccacggg tcgtccgtcc ggagatttcg tgtcagaaga
      961 tgaacttggg caggaaatcg atgatgccgc cggtctcccg gatcacgtag tggaactcgt
     1021 cgcccgacca ggacttgatc gggctgttct tcgccggcga tccgtacgcc gtgaagccga
     1081 gctcggtcgc catcaacttc gagcgggcaa ggtggaagcc gtcgctgacg agcagcagct
     1141 tcttgactcc ggttcccttc aggactgcgg cgacgccctg catgctctgc caggtgtcgc
     1201 gccccttgtt ctcgtagagg atcgcgctcg acggcacgcc gttatccatc aggaagttcg
     1261 cttcggattc cgcttcggtg taggcgtcgc ccggcatctt gccgccggtg accaccacgc
     1321 gcggcgcgta gccgtcgttc cacaaggcaa gcgcctgatt gagtcgcgcc tggagaacgg
     1381 ggctcggcgt gccgttgtac tgcgcggcgc cgaggatcac gatcgcgtcg acacggcgcg
     1441 cctggtcgtt gtgcgcctcg taatagacgt agcccatcac cgcgaggatg aagagcggga
     1501 ccgcgaaggt gacgaagagg atgagccgga ccatccagcg gccgaagcca gccttcttgc
     1561 gccgggtccc gatcggggga gcgatggttc gcagcgcgcc ctccggacct ggcgtctgtg
     1621 ccggccaggt cgccgagtac gactcctgtc ttggcgaggg agactgcggt gggcgcgcgg
     1681 cgtgaggtgg aggagtgcga tcggaatcgc cagcgtttcg catgcgcgaa gcatgtcacg
     1741 ccgcgtttct cccgtgcaac tcacgccgcc gtggcgatca tgattcgacg gcaaccaggc
     1801 ggatcgactc gacggcgtcg ggattctcga gcgccgacgt atcgcccgcc ggcagcccca
     1861 gccatgccgc ccggatcacc cgccgcatga tcttcgcgtt tcgtgtcttg gggaggtcgt
     1921 tcacgaagac gacgcggtcc ggccgcaacg ccgcgcccag ctgattgccg atgaagcgct
     1981 tgatctgctc ggccagctcg tccgtcgccg tctggtcgga gcggagcacg cagaagacga
     2041 tcgcgacgtc gcccttcacc ggatgcggca cgccgatcgc cgccgcctcg cgcaccgttc
     2101 cgtgcgcgac cgccgcgctc tcgacttcgg cggggccgat ccgtttcccc gcgaccttca
     2161 acgtatcgtc ggagcgaccg cggatgtacc agaagccgtc ggcgtcgatc tcggcgaagt
     2221 cgccatgaac ccagacaccc ggcagtcgcg accagtacgt ttcctcgtag cgatcgggat
     2281 cgcgccagaa gccgttcgtc atcccgaccc acggctcgcg gatcacgagt tcgccgacac
     2341 cgtcccggac tggcgcgcct cccgcgtcaa caaccacgac atcggccacc atcccgggaa
     2401 tcgggccggt gaacgagcat ggcttgatcg ggaagatcgg caggctcgag atgatcccac
     2461 ccgacgtctc ggttccgccg gagtagttga tgatcgggca acgcccgtca ccgacgacat
     2521 cgaggaacca gcgccatgga tcgggattcc aggtctcgcc cgtggagccg aggatccgca
     2581 gcgacgaacg atccgtcgcc ttcacgatct cctcgccgta ctggatcagc gcccggatcg
     2641 ccgtcggcgc cacgccgagc gtcgtcaccc ggtgcttctc gacgatccgc cagaggcgat
     2701 cggaggttgg atagtccggc gtcccttcga agagcaggat cgtggctccc agcgtcagac
     2761 caccggcgat cagccacgga cccatcatcc agcccatgtc cgtcatccag aacagcacgt
     2821 cgccctgatg gagatcgaag ctgtaggcga gatcgtgcgt cgccttgatc gggaaaccgg
     2881 cgtggatgtg cacggctccc ttcggacgac ccgtcgtgcc ggatgtgtag aggatcatgt
     2941 agggatcgtc ggccgccgtc ggctcggtga tccgcgacga atcggccgtc gccatcgcct
     3001 cgtcccacca gacgtcgcgc ccctcggtcc agtcgatctc ggcacccgta cggcgcacaa
     3061 cgacgacgtg ctcgatcaac ggcagtcgtg cgagcgcctt gtcgacgacc gccttcatcg
     3121 gcacgaactt gccacgccgg aggaagccgt cggcgacgat catcaccttc gcgtcggagt
     3181 cgccaatccg cgagacgatc gattcctcgc cgaaaccgga gaagagcggc acgtagatcg
     3241 cgccgagctt gccgcaagcg agtgtcgccg cgacgacttc cgggatcatc ggcaggaaga
     3301 tcccgacctt gtcacccttg ccgacaccga gaccgcgcag gacgtgcgcg aagcgatccg
     3361 tcgtctcgcc gagttcggcg aaggtcaggg ttcgcgtcga ctcgtcgtcg ccttcccaga
     3421 tcacggctgg ctgatcggcg agcgtaccgt cggcgtgacg atcgagcgca ctcgcgacgt
     3481 agttgaactc ggcatccgga aaccagcgcg gccacgcctt gcctcggctc agatccatga
     3541 tctgcgacca cggcttctgc caggcgagat cgaggtcggc cagcgcctgc tcccagtacc
     3601 aggtcggatc ctccgccacg cgcgcgagaa gtccctggta gtccccgctc cccgtcttct
     3661 cgatgaggcg caggagacgg ttatcccgca ggtaatcctc ggttggaatc caggccacgt
     3721 ccgacatcgt catgagatga ctcccgggtt tcgcgcattc atgctggcaa tgacttcgta
     3781 ggcgtattgt tcccagcgga atcccgttcg gtcaagggct tcgtgcatcg ccgggtcgtt
     3841 gtgggagaat tcccgcagtg aactccctgt gaaagcgacc aaagatgcct tggcgacaat
     3901 tctggaaacg cgagaagcaa gcagagagcg ggcaggaggc gaagtccgcc cctcccgcca
     3961 ctgagccgac cccatccgtt cccgcgtcga atctgccacc ccacatgcaa cggatcgtcg
     4021 gcgaacgcga gcggccggcg aacagaccgc cgctgaatcc cgccgagaag cgtgcgcgcc
     4081 tccagcgaca acggctcgcg atcctcttcg atgtcgatca gggcgagctc gccgagcagg
     4141 acgacaatcc atggtcgagc cggatctccc tcctgacgga cgcgctcgag accgtcaacg
     4201 tcgaccgcgc gcggctcctc aggatcgcac cgtccccata cgcaccggta cccgcgacac
     4261 cgatcgccga tctcaccatc agcgagacgc agccgattgc gatctccttc caaattggac
     4321 cggagaggtt cgcctacgag gaagatctcg actgggcgga acgggggcac cagctggcgc
     4381 tgccagagct tcatcagaag acgggatgtg tcgacgccat catcccgcag gatttcccca
     4441 acgatctccg cgagccgctg gccgcccatc tttcccacag tctcgacgtc ttcgcggcag
     4501 acctccgcga tcgccggctc gacgacgagc cgctgccaga atcgccgaca ctcgcggaca
     4561 tcgcacgccc gtgcccaacc tgcggcggct ggaccgactg gaagggtcgc tgcgcagcgt
     4621 gcgccgcccg cgccgcggac gaactccagc tgaaccgcga gcaacgacgg ctcctcgatg
     4681 agcgcgcccg ggaggcggaa gaacgacatc gcctgcgcga ccgcttgccg ctcgcccgga
     4741 agcgcctggc cgatgtcgat gccgagatcg cggcgctcga tgaatgacgc cctgacgcac
     4801 atcgcccgcg gcctcgtcga tcgccacacg ttcggcgtgg tcatgctcgt cgcgatcgcg
     4861 gcgattggct ggcggaatcg gtcctccttc cggggcatgc tcgccgacga tgacgccttc
     4921 tggcgactca cggcgcggct cttcgtcggc tcgttcgtcg cgaccgcggt ctggatcgcg
     4981 atcttcgacg actggatcca gctcttctcg gagccttatc gccgcacccg cgatctctcg
     5041 gtcgatcgcg tcgttgccga tccgatcccg accgaactcc gggcgatcac gctgatcctg
     5101 ctcgcgatca ccatcatcgc aaccgcggca atcgttgccc gccacatcgg aggatacggc
     5161 ctgcagatcg gggcgcttct gctcagcatc accttctggc tgatcctctt catcttc
//
LOCUS       DASTMD010000463         4386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_222446, whole genome shotgun sequence.
ACCESSION   DASTMD010000463 DASTMD010000000
VERSION     DASTMD010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222446"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VFQ54_06740"
     CDS             <1..1333
                     /locus_tag="VFQ54_06740"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEU0164727.1"
                     /translation="SLPSGAVLVGTDRGVAEVVSMPQSGNGAERVRISVETAGDVTLT
                     KPVDVIAFLPRGVEVNVGDRIALAWSFEHVDTLAPGFGGWVTSQGVVGVVYVYEVTIE
                     ARGDSWERPLVDARRLVSDRLQRLIPGDAGALASGIVTGDDSRMSAEVNGAFQRTGTA
                     HVTAVSGQNVAMLIGLAALLFPARWRKRAVMIQAALVVLIVSYALMVVFNPPSVRAAF
                     FAILVLFGARFGRRPDLFTILALVTAGMVLWNPGMVQSISFWLSVVSSAALVTCLEHE
                     LGAGWKRKVGGVIVGLAAAQLSTVPITLAAFGTWSIGSIFANLIIAPIVALAFPVTFV
                     VALVAVASPTAARWIGWFPEILLRFVIDVVTEIDRTFGVVDFAASRELGVIAIAVPCG
                     MGLLVLSGDARRWLDRVRQSPFRESSGGRAAIAGASMGLGLAIAVAILGFH"
     gene            1370..1443
                     /locus_tag="VFQ54_06745"
     tRNA            1370..1443
                     /locus_tag="VFQ54_06745"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1404..1406,aa:Glu,seq:ttc)
     gene            1490..1987
                     /locus_tag="VFQ54_06750"
     CDS             1490..1987
                     /locus_tag="VFQ54_06750"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0164728.1"
                     /translation="MTELKTAEDVITLLDLQPLPGEGGFFRQTYVRSSTDPDGPPETT
                     AIYYLVTPDSFSGLHRLGHDELFHFYLGDPCEMVTYIDGEQPEIIVLGQDLGAGMTVQ
                     HLVPAGTWQGTRLRDGGRFALMGTTMTPGFDPRGFELASPDLLAGVPDVAAEQLSRFL
                     AHASH"
     gene            2204..3181
                     /locus_tag="VFQ54_06755"
     CDS             2204..3181
                     /locus_tag="VFQ54_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU0164729.1"
                     /translation="MDIILPAAGFGTRLRPQTWSKPKPLVTVAGKPMIEHVLDRLMPY
                     GPEKLVFIVGYLGDQLETWARSTYTDVEVVVKYQPQMLGQSDAILRTRDVCRDDALIL
                     FPDALFEADFSGLAELDVDGVAYTKVIDDPSAYGIAVVEDGRITRLVEKPSEPISNLA
                     LIGIYYFKQVQDLYAAMDDQIAREIKTRGEYFLSDAVEIMVENGKHFVTKAVESWEDC
                     GNTEALLQTNRYLLSHDSPSPVQRPGSVINHPSFVHREAVLENAVVGPYASIGAGSVI
                     RNAVLRDVIVGDNSTVEDVVLERSIIGDGVEVSGSAATINIGDAGKIAL"
     gene            3196..4152
                     /gene="prmA"
                     /locus_tag="VFQ54_06760"
     CDS             3196..4152
                     /gene="prmA"
                     /locus_tag="VFQ54_06760"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU0164730.1"
                     /translation="MSESVNAATTAPEKIWIELAVEVDAEAVEPVSELFGRYGFNEGV
                     AIDEPYVQDGDGDNLEIATDRPFVIRTYVADSDFKPEIIEQVKEGLWYLGKLRSIGEL
                     RISNLKEEDWANAWKEHFQVHRIGERVVIRPTWREYAAQGDEVVVDLDPGMAFGTGLH
                     PSTRLSMLGLETVVKPGDAVLDVGTGSGILAIAALKLGASRADTVDVETVAVRATAEN
                     AALNGVADRLKVELGSVGTGEPFDGEQYDVVLANIIARILIDLSPAIVGHTRPGGSMV
                     LAGIIQQREDDVVAAFAAQGAIVVQRRQFDDWVSLVLERSQG"
BASE COUNT          743 a         1313 c         1457 g          873 t
ORIGIN      
        1 ctccctgccg tccggggctg ttctggttgg aaccgatcgg ggagtcgcgg aagtcgtttc
       61 gatgccccaa tcggggaacg gagccgagcg cgttcggatc tccgtcgaaa cggccgggga
      121 cgtcacgctg acgaagccgg tcgatgtcat cgcgtttctg ccgcgcggtg tcgaggtcaa
      181 cgtcggcgat cggattgcgc tcgcctggag cttcgagcat gtcgacacgc tcgcacccgg
      241 tttcggcgga tgggtgacgt cgcagggggt tgtcggagtc gtctacgtct acgaggtcac
      301 gatcgaggct cgcggcgact cctgggaacg cccgctcgtc gatgcccgcc ggttggtcag
      361 cgatcgcctg cagcggctga ttcccgggga tgcgggcgcg ctggcgtcgg gaatcgtcac
      421 cggcgacgac tcgcggatga gtgccgaggt caacggagcc ttccaacgca cggggacggc
      481 acatgtcacc gcggtgagcg ggcagaacgt ggcgatgctg atcgggcttg cggcgttgct
      541 ctttccggcc cgatggcgga agcgggccgt gatgatccag gccgcgctcg tcgtcctgat
      601 cgtctcctat gcactgatgg ttgtattcaa cccgccgtcc gtgcgcgccg cattctttgc
      661 cattctcgtc ctcttcgggg cgcgattcgg acgaaggccg gatctcttca cgatcctggc
      721 gctcgtcacc gccggaatgg tgctctggaa cccggggatg gtccagtcga tctcgttctg
      781 gctctccgtg gtttcttcgg cggcgctggt gacctgtctc gaacacgagc tgggagccgg
      841 ctggaagcga aaggtgggag gtgtaatcgt cggattggcc gcggctcagc tctcgacggt
      901 gccaatcacg ttggcggcgt ttggcacatg gtcgatcggc agcatcttcg ccaacctgat
      961 cattgccccg atagtcgccc tcgctttccc cgttacgttc gtcgttgcgc tcgttgccgt
     1021 tgcttcgccg acagcggcga ggtggatcgg ctggtttccg gagatcctgc tgcgtttcgt
     1081 gatcgatgtc gtgacggaaa tcgatcggac gtttggggtc gtcgacttcg cggcgtcacg
     1141 ggaactcggc gtgattgcga tcgcggtgcc ctgcgggatg ggattgcttg tcctgagcgg
     1201 cgatgcgcgc cgctggctcg atcgggttcg gcaatcgccc ttccgggaat catcaggagg
     1261 tcgtgccgcg atcgctggcg cctcgatggg gctggggctc gcgattgccg tcgcgatcct
     1321 ggggtttcat tgacaaagtg cacattcagc gcgtatcttg tccggcgtcg cccccatagt
     1381 ctagcggcct aggacgtcac cctttcaagg tgaagatcgc gggttcgaat cccgctgggg
     1441 gtacccaccc acagtccggc gacaactccc ctcccaacag acgagatcaa tgaccgagct
     1501 gaagaccgct gaagacgtga tcacgctgct tgatttgcaa ccgcttcccg gtgaaggcgg
     1561 tttcttccgg cagacgtacg tccgttcctc cacggacccg gacggccctc cggagacgac
     1621 cgcgatctat tacctcgtga cgccggattc cttctccggt cttcatcgtc tcggccacga
     1681 cgaactcttc catttctacc tcggcgaccc ctgcgagatg gtgacgtaca tcgacggcga
     1741 acagcctgag atcatcgtcc tcggccagga tctgggcgcc gggatgaccg tgcagcatct
     1801 cgtcccggcg ggaacctggc agggaaccag gttgcgcgac ggcggtcgat tcgcgttgat
     1861 ggggacgacg atgacgccag gattcgatcc gcgtggtttc gagctcgcgt cgccggacct
     1921 gctcgccggc gtaccggacg tcgctgccga gcaactgagc cggtttcttg cgcatgcaag
     1981 ccactaagac accgccgctc ctgccgcagg tggggtatcc tgtccagaat tggtgcaggt
     2041 gaatgatggt cagcttcgcg ctgtccgtcg catgcgaccg ctccacgaca agccgttgtc
     2101 cgctggggtg agttggtcat gtgcgccgtt cggaaaacca cgatggatcc gcggcgattg
     2161 gctgagattc cgggatctgt caatatcttg tgggagggat tgcgtggata tcattctgcc
     2221 tgcagcgggg ttcgggacac gactgcgacc gcagacgtgg agcaagccga agccgctggt
     2281 gacggttgcc gggaagccga tgatcgagca tgtgcttgac cgtctcatgc catacggccc
     2341 ggaaaagctg gtcttcatcg tgggatacct cggtgaccag ctggaaacat gggcacgctc
     2401 gacgtatacc gatgtcgagg tcgtggtcaa gtaccaaccg caaatgcttg ggcagtctga
     2461 tgcgattctg cgcacccgtg atgtctgccg ggacgacgcg ctgatccttt tccccgatgc
     2521 cctcttcgag gccgatttct ccggtctcgc cgagctcgac gtcgatggcg tggcatacac
     2581 gaaggtgatc gatgacccgt cggcgtacgg gattgctgtc gtcgaggacg gcaggatcac
     2641 gcggcttgtc gagaagccgt ccgaaccaat ctccaatctc gccctgatcg gcatctacta
     2701 cttcaagcag gtccaggatc tgtacgcggc gatggacgat cagatcgcgc gggagatcaa
     2761 gacgagggga gaatactttc tctctgatgc cgtcgagatc atggtcgaga atggcaagca
     2821 cttcgtcacc aaggccgtgg agtcctggga ggattgcggc aacaccgagg cattgctcca
     2881 gacgaaccgg tatcttctct cccacgattc gccgtctccc gtccagcggc cgggcagcgt
     2941 catcaaccat ccgtcgttcg tgcatcgcga ggcggtcctc gagaacgccg tcgttggtcc
     3001 gtacgccagc attggcgccg gttcggtgat tcgcaacgcg gtgcttcgcg atgtcatcgt
     3061 gggcgacaat tcgacggtcg aggatgtggt gctcgagcgc tcgatcatcg gcgatggcgt
     3121 tgaagtgtcc ggcagcgcgg cgacgatcaa tattggcgat gcgggcaaga tcgcgctcta
     3181 gtaattgggg aatcaatgag tgaatcggta aacgcggcaa caacggcgcc ggagaagatc
     3241 tggatcgagc tcgcggtcga agtcgatgcg gaagcggtcg agccggtctc ggagctcttc
     3301 ggtcgctacg gcttcaacga gggcgtcgcc atcgacgagc catacgttca ggatggcgat
     3361 ggcgacaatc tcgagatcgc gaccgaccgg cccttcgtca tccggaccta tgtcgcggac
     3421 agcgatttca agccggagat catcgagcag gtcaaggagg gactctggta cctcggcaag
     3481 ctgcgctcga tcggtgagct caggatctcg aatctgaagg aagaggattg ggcgaacgcc
     3541 tggaaggagc acttccaggt tcaccggatc ggtgagcgcg tcgtgattcg cccgacctgg
     3601 cgcgaatacg cggcgcaagg cgacgaggtc gtggtcgatc tcgatccggg aatggcgttc
     3661 ggcacgggac tccacccgtc gacgcgcctg tcgatgctgg gtctggagac ggtcgtcaag
     3721 cccggcgatg ccgtcctcga tgtcggaacc gggtcgggca tcctcgcgat cgccgcgctc
     3781 aagctcggcg cgtcccgcgc cgataccgtc gacgtcgaaa ccgtcgccgt tcgcgcgacg
     3841 gcggagaacg cggcgctgaa cggcgtcgcc gatcgtctga aggtggagct cggcagcgtt
     3901 gggaccggcg agccgttcga cggtgagcag tacgacgtgg tgctcgccaa catcatcgcg
     3961 cgcatcctga tcgacctctc gccggcaatc gtcggtcaca cccgaccggg tggctcgatg
     4021 gtgctcgccg gcatcatcca gcagcgtgaa gatgacgttg tcgccgcttt tgcggcgcag
     4081 ggagcgatcg tcgtccagcg ccgtcagttc gacgactggg tttcgctcgt gctggaacgc
     4141 agccagggct agccatctcc cgagcctgga aatggacgtg ccgtccttcg gatgaaggac
     4201 ggcacgtcgc gttcggcgac agataacgca tcaggaaatg ctggcaccct gcgttctatt
     4261 tgccttcggc gatcgatgac gtgtcgggag acgctagcgg tgccggcgga ggaagcggcc
     4321 gacgatgaag acggcgaccg cgctccccgc gccgagcaac gcgtctccca acgcgtagta
     4381 gggacg
//
LOCUS       DASTMD010000464         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2543780, whole genome shotgun sequence.
ACCESSION   DASTMD010000464 DASTMD010000000
VERSION     DASTMD010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543780"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(381..668)
                     /locus_tag="VFQ54_06765"
     CDS             complement(381..668)
                     /locus_tag="VFQ54_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164731.1"
                     /translation="MYPMLPSETQADLSHELFNGKRSHEWPDDPFYQIPLESAALRPD
                     PIWAALWEDLAALGGGIRRGIAWIRRRPGSTTTLAQPTTGCDREGRPYGMS"
     gene            890..1282
                     /locus_tag="VFQ54_06770"
     CDS             890..1282
                     /locus_tag="VFQ54_06770"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0164732.1"
                     /translation="MKYVLLIYSNPETWRHPSYKYISEAKSAEKLAEMQAKSDELTRQ
                     FEATGELLYAEPLATPSRAKLGRTKGGTPVVTDGPFLESKEQLAGYFVIDVATPERAY
                     EIAALFPETEYSPIEVRPISGPEDMDDE"
     gene            complement(1324..>2091)
                     /locus_tag="VFQ54_06775"
     CDS             complement(1324..>2091)
                     /locus_tag="VFQ54_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164733.1"
                     /translation="VAPGAAGLLIQLVTAPKAILADVGSYLASAWALGGVGTGEAKPI
                     RQSSGSVLADIGREIREGLHELVRTPLLRSLAISMGVIVVGGSVQQTVQVLFFTHTLH
                     LSAFEIVSLGVFNGIGSLIGAALAGKVALRWPIGMVMIGAAISEGIVVGTSAFAGLAP
                     YPYVVLAGTATAGGLGYAIFAITQISYRQRITPTQLMGRVTSARRFLIFCMGIFGTAL
                     GGWAGTVLGFQPALLIGAAIMFGGPLVMWFSPVRDAA"
BASE COUNT          404 a          721 c          651 g          315 t
ORIGIN      
        1 gtcgaagggg cggctccgaa gccgtcagcc cggagagagt ctcccggcga aggtgatgcg
       61 gggctgatcc gtcgcgacgg ggacggtccg cgggccagca ccaggcggga cattcctccg
      121 tccagcgacc ggagtttccg cgccaaagac gcgccgagcg ccggtcgcgg gactccgtag
      181 tcccgcccca catacgcacg cggcatccat gactggtccg gtgtgccgat gaaccgcccg
      241 ccctttcccg ccttcgcccc actccctcgc gatcccgcca caacgtaaac gcggagagac
      301 cggcaggatg cgagatgcct gccggtctct tgtcgccgtc ctgcgcgcgg tcatctgccc
      361 ttccgccgtc cggcaaccac ctacgacatc ccgtagggac gcccttcccg atcgcaccct
      421 gtcgtaggct gagccagggt cgtcgtcgat ccgggacgcc gtcgaatcca ggcgatgccg
      481 cgacggattc caccgccgag cgccgccagg tcctcccaca gcgccgccca gatcggatcg
      541 gggcgaagcg cggcgctctc caggggaatc tgatagaagg gatcgtccgg ccactcgtga
      601 gatcgcttgc cattgaagag ctcgtgcgaa aggtcggcct gggtctcgga cggaagcatc
      661 gggtacatct cggtcactcc ttgcagatgc tgtactgctt gcggaaccct cgcttgggtc
      721 cctctgcaaa ccagtcgtct ccaggcgcga catctcgaca tttctgcgat gacgattttc
      781 tcgacgatcg tgatgtccga aaatgtagag gtcaccacaa aggatccgac caccgtgtag
      841 agggacatcc gatggggatg tccctggcag caccaaaggg agtgacacga tgaaatacgt
      901 gctcttgatc tacagcaacc cggagacctg gcgacacccg tcctacaagt acatctccga
      961 ggcgaaatcc gcggagaagc tggccgagat gcaggcgaag agcgatgagc tgacccgtca
     1021 gttcgaggcc accggcgagt tgctctacgc ggaaccactc gcgacgccga gtcgggcaaa
     1081 gctcggccgc accaagggcg gcacgccggt cgtcacggat ggaccgttcc tcgaatcaaa
     1141 ggaacagctc gccggctatt tcgtgatcga tgtcgcgacc ccggaacgcg cctacgagat
     1201 cgcggcgctc ttccccgaga cggagtacag cccgatcgaa gtccgcccga tctccggtcc
     1261 cgaggacatg gacgacgagt agtcgaagcg gtcatcgatg ccgtggcatg agggcgctcg
     1321 ccctcatgcc gcgtcccgga ccggcgagaa ccacatcacc aatggccctc caaacatgat
     1381 cgcggcgccg atcagcagtg ccggctggaa tcccagcacg gttcccgccc atccgccgag
     1441 cgcggtgccg aagattccca tgcagaagat gaggaagcgc cgcgccgagg tcacgcgccc
     1501 catcagctgc gtcggcgtga tccgctgccg gtagctgatc tgcgtgatcg cgaagatcgc
     1561 atagccgagc ccaccggcgg tcgccgttcc cgccagcacg acgtagggat atggggcgag
     1621 gccggcaaag gccgaggttc caacgacgat cccctccgag atcgcggcgc cgatcatcac
     1681 catcccgatc ggccagcgga gcgccacctt gcccgcgagc gcggcgccga tcagcgagcc
     1741 gatcccgttg aagacgccaa gcgagacgat ttcgaacgcg gagagatgca acgtgtgcgt
     1801 gaagaagagg acctggacgg tctgttgcac cgatcccccg accacgatca cgcccatcga
     1861 gatcgccagc gaccgcaaca acggcgtgcg cacgagctcg tggagcccct cgcgaatctc
     1921 ccgcccgata tcggcgagca cacttcccga cgattgccgg attggtttcg cctcacccgt
     1981 gccgacgcca ccgagcgccc acgccgaggc gagataggag ccgacgtcgg cgagaatcgc
     2041 cttcggcgcg gtcacgagct ggatcagcaa tccggcggcc cccggtgcga c
//
LOCUS       DASTMD010000465         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2006954, whole genome shotgun sequence.
ACCESSION   DASTMD010000465 DASTMD010000000
VERSION     DASTMD010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006954"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1551)
                     /gene="acnA"
                     /locus_tag="VFQ54_06780"
                     /pseudo
     CDS             complement(1..1551)
                     /gene="acnA"
                     /locus_tag="VFQ54_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
BASE COUNT          249 a          479 c          554 g          272 t
ORIGIN      
        1 gacggtagcg tattcctcgc ggaacatcgc agggtcgatc gaactctcga tcgcggcctg
       61 gacctcctgt tcggttggcc agatgtcggc gaggtagacc ggatcgccat tcggatcgta
      121 gccgaccggc tctgtcgtca gatcggtatc gaccgaaccc gcgagcgcgt aggcgacgac
      181 cagcggcggc gaggcgagga aggaggcgcg cacctgtggg tggacgcgtc cctcgaagtt
      241 gcggttgcca gagagcaccg aggcgacgac gaggttgttc tcatccaccg ccctggcgac
      301 ggcatccggc aggggacccg agttgccgat gcaggtcgtg cagccgtagc cgacggtctg
      361 gaagccgaga gcgttcaggt atggcgtcac accggagcga tcgaggtagc gggtaacgac
      421 ctgcgatccc ggcgcgagac tggtcttcac gtacggcggc acgtcgagac cgcgctagac
      481 cgccttcttt gcgagcagac cggcgcccag catcacggac gggttcgacg tgttggtgca
      541 ggaggtgatc gccgcgatcg cgaccgcgcc atgatggagg tcggtctcca tgccgtcgat
      601 gtggatcgag acctcgccga agtggctgac cttcttcggc ttcgcgacaa ccgcgagacc
      661 gcctgccggt tccgggtcga ggtgggcgtc tccaccaccg gccatcgatg ccgggggatc
      721 gctcgccgga aaggattccc cggcctggat gtcgtaggcg gtgtcggacg tctcgagtgc
      781 tgccgggaag gccgcgtcgt acgtcgcgtg cagggtgcgc tggacatcgc cgagcgcgat
      841 ccgatcctgc gggcggcgag gaccggccat gctcggctcg acggtggcga gatcgagttc
      901 gagaagctcg ttgaagatcg gatccggtgt ctcgtcggtg cggaagaggt tctgcgcctt
      961 cgcgtagagc tcgacgagtt ctacctgatc gtcggtgcga ccggtcaggc gaaggtagcg
     1021 cagcgtctcc tcgtcgatcg ggaagagccc ggcggtcgcc ccgtattccg gcgccatgtt
     1081 cgcgatcgtc gcgcggtcgg cgaggctgag gttgctgagc ccggtgccgc agtactcgac
     1141 aaaacgaccg acgacgccgt gcttgcggag gagttgcgtc acggcgagga cgagatcggt
     1201 cgccgtcgta cccgggcgca tgctgccgat gaacttgagg ccgacgactt ccggcgagag
     1261 ctggtagagc ggctgaccga gcatcacggc ctcggcctcg atgccgccga cgccccagcc
     1321 gagcacgccg agcgcgttga tcatcgtcgt gtggctgtcg gttccgacga gcgtatccgg
     1381 gaatgcgacg acgccatcct cggtctcctt cgtcgcgatc accggcgcca ggtactcgag
     1441 attcacctgg tggacgatgc ccgttcccgg agggacggcg cggaagttct ggaaggcgtt
     1501 ctgtccccag cgaagcagcg tgtagcgttc ctggttccgc tcgtattcca gctc
//
LOCUS       DASTMD010000466         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2364797, whole genome shotgun sequence.
ACCESSION   DASTMD010000466 DASTMD010000000
VERSION     DASTMD010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2364797"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2088)
                     /locus_tag="VFQ54_06785"
     CDS             complement(<1..2088)
                     /locus_tag="VFQ54_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU0164734.1"
                     /translation="MSHGRITRASRRYPDAKRRAARYGRTPYGKRTTSLPPHLLPGAA
                     GRGKKAGPITITRALFILGAIFVATTISIVILTVVSGVAGAYGTIRAYKDVNKKLPNA
                     AQVSAKTFQTTRLYDRNGTLLQEVDNPNFGWRTYVGYDQISPHLINATVSAEDSTFWT
                     NEGVEPIAIIRGGFINFSGAGSSGGSTITQQLVRAIYPDQISALDISYTRKIREAMAA
                     IALAKKYSKKDIITMYLNQIYYGARSYGIEAASQTYFNKHAKDLDLAEASMLAGLPQA
                     PSYYEPDIPENFAIAKRRQQYVLDQMVKYRYITKEDADAAFAEALHPKNDRSGAVLNA
                     PHFTQYVKDYIIDHYGEDALYGGLEITTTIDLPLQAEAEQIVATGVQNMAQYDRNNGA
                     VVVISPWSGQVLCMVGSADFNDALIDGQVNFATSQIQPGSSMKPIVYSAAFEKGWNPA
                     TILLDVPTTWKIEGQPDYTPQNDLGIFYGAVSVRIALDNSLNIPALKTADFLGVQSVI
                     DQAHKMGIKSSLNEGAGFYGLSIALGAGEVTLLEHTNVYATLANNGTYVPANPILQIK
                     DSQGNLLYKLDEAQVNKTKTQAVPAGNAYQITSILTDNDSRKLVWGLDNLFGNTQTSI
                     SRPTAVKSGTTENWKDLWTMGYTTAAAIGVWVGRSGDTSDTALPEIDGVVAAGPIWQD
                     MMELIHKNPTWLKL"
BASE COUNT          338 a          614 c          711 g          445 t
ORIGIN      
        1 gagcttcagc catgtcgggt tcttgtggat caactccatc atgtcctgcc agatcggtcc
       61 ggcggcaacg acgccatcga tctccggcag ggcagtgtcc gacgtgtcgc cgctgcggcc
      121 aacccagacg ccgatcgcgg cggcggtcgt gtagcccatc gtccagagat ccttccagtt
      181 ctccgtcgtt cccgacttta cggccgtggg gcgactgatc gaggtctggg tgttcccgaa
      241 gagattgtcg agtccccaga cgagcttgcg cgaatcgttg tcggtgagga tcgaggtgat
      301 ctggtaggca ttgccggcgg gcaccgcctg ggtcttggtc ttgttcacct gcgcctcgtc
      361 gagcttgtag aggagattcc cttgcgagtc cttgatctgg aggatcggat tcgccggcac
      421 ataggtgcca ttgtttgcga gcgtcgcata gacgttcgtg tgctcgagca gcgtcacctc
      481 gccggcgcca agggcgatcg agaggccgta gaaccccgca ccctcgttga ggctgctctt
      541 gatccccatc ttgtgcgcct gatcgatcac cgactgcacg ccgaggaagt cggcggtctt
      601 gagggcgggg atgttcagcg agttgtcgag tgcgatccgg accgacacgg ccccatagaa
      661 gatgccgagg tcgttctgcg gggtgtaatc cggctggccc tcgatcttcc aggtggtcgg
      721 gacgtcaagc agaatcgtcg ccgggttcca gcccttttcg aacgcggccg aatagacgat
      781 cggcttcatc gaggaacccg gctggatctg gctcgtcgcg aagttcacct gaccgtcgat
      841 cagggcgtcg ttgaaatcgg cggagcccac catgcagaga acctggccgc tccacggcga
      901 gatgacgacc actgcgccgt tgttgcgatc gtactgcgcc atgttctgga cgccggtcgc
      961 cacgatctgc tcggcctccg cctggagcgg gagatcgatc gtcgtcgtga tctcgaggcc
     1021 gccgtagagc gcatcttcgc cgtagtggtc gatgatgtag tccttgacat actgcgtgaa
     1081 gtggggggcg ttgagaacgg cgccggagcg atcgttcttc gggtgcagcg cctcggcgaa
     1141 cgcggcgtcc gcgtcctcct tggtgatgta gcgatacttc accatctggt cgagcacgta
     1201 ctgctggcgg cgcttcgcga tcgcgaagtt ctccgggatg tcgggctcgt agtaggaagg
     1261 cgcctgcggc agacccgcga gcatcgaggc ttcagcgaga tcgagatcct tcgcgtgctt
     1321 gttgaaatag gtctgcgacg ccgcctcgat cccgtatgac cgcgcgccgt agtagatctg
     1381 attcaggtac atcgtgatga tgtccttctt cgagtacttc ttggcgagcg cgatcgcggc
     1441 catcgcctcg cggatcttgc gggtgtagct gatgtcgagc gccgagatct ggtccgggta
     1501 gatcgcgcgg acgagttgct gggtgatcgt cgaaccgccc gagcttccgg cgccggagaa
     1561 gttgatgaag ccaccgcgga tgatcgcgat cggctcgacg ccctcgttcg tccagaaggt
     1621 ggagtcttcg gcggagaccg tcgcgttgat caggtgaggc gagatctgat cgtatccgac
     1681 gtaggtgcgc cagccaaaat tcgggttgtc gacttcctga agcagggttc cgttgcgatc
     1741 gtagagccgc gtcgtctgga aggtctttgc ggagacctgt gccgcgttcg ggagcttctt
     1801 gttgacatcc ttgtaggcgc ggatcgtgcc gtaagcgccg gcgacgcccg acacgacggt
     1861 gaggatgacg atgctgatcg tcgtcgcgac gaagatcgcg ccgaggatga agagcgcgcg
     1921 ggtaatcgtg atcggcccgg ccttcttgcc acgtcccgcg gctccgggca gcagatgtgg
     1981 cggcagcgac gtcgtgcgct tgccgtatgg ggttctgcca taccgcgcgg cgcggcgctt
     2041 cgcgtcggga tagcggcggc tcgcgcgtgt gattcgacca tgactcatga agtggacgac
     2101 tccctcgg
//
LOCUS       DASTMD010000467         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1026241, whole genome shotgun sequence.
ACCESSION   DASTMD010000467 DASTMD010000000
VERSION     DASTMD010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026241"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VFQ54_06790"
     CDS             complement(<1..1187)
                     /locus_tag="VFQ54_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEU0164735.1"
                     /translation="MTNWPRFTMASGPVDIAPETLRAMQRPALYHYDPRFIEIFENAN
                     RLLKAAFRTDYDTVIMQAEAVLGLEAAAASLIEPGDKVLNLVSGVFGKFFEDWIRKYG
                     GEPIELAVPYNAAIDPEDVRCVLQTQPGIKYLSVVHSETPSGTVNPMKEIGRIAHEFG
                     VLTIVDTVSGWLSEDLSPEEWGMDVAVAGPQKCLGSTPGLSLITVSPAAWEAMEIRPN
                     PLRGSFLSLLDWKSSWIENHRFPYTPSVYEMYAVESALQQVIDEGVANVVARHQVSAK
                     ASRAAVRALGLDLWPESDEIAATCCTAVTAPEGLDEGKLRAIMRSRYGVMISGGYGSI
                     SGKVFRLGHMGPSAHPTAVVSQLGILERSLDDLGFRIPLGAGVGAALAEFKGWNDEER
                     QYP"
     gene            1331..>1516
                     /locus_tag="VFQ54_06795"
     CDS             1331..>1516
                     /locus_tag="VFQ54_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164736.1"
                     /translation="MPRLAANLTMLFTEVPFLERFDHAAAAGFKGVEFLFPYSETPEA
                     IQDALTKNGLELVLFNLP"
BASE COUNT          273 a          530 c          442 g          271 t
ORIGIN      
        1 ctcgggtact ggcgttcctc gtcgttccat cccttgaact cggccagagc ggcgccaacg
       61 ccggccccga gcggaatccg gaatccgaga tcgtcgagcg atcgctccag gattccgagc
      121 tgtgagacca cggccgtcgg atgcgccgat ggccccatgt gaccgaggcg aaaaaccttt
      181 cccgatatcg agccgtagcc cccggagatc atcaccccat accgcgagcg catgatcgcc
      241 cggagcttgc cctcatcgag tccttccggc gccgtcaccg ccgtgcagca ggtggcggca
      301 atctcgtcgc tttccggcca caggtcgagc cccaaggcgc gcacggccgc acgtgacgcc
      361 ttcgccgata cctggtgacg cgccacgacg ttcgcgacgc cctcatcgat cacctgctgc
      421 aacgcggact ccaccgcata catctcgtag acggatggcg tgtacggaaa gcgatggttc
      481 tcgatccagc tcgacttcca gtcgagcagc gagagaaagc tgccgcggag cggattcgga
      541 cgtatctcca tcgcctccca cgccgccgga ctcaccgtga tcagggagag ccccggagtc
      601 gatccgaggc acttctgcgg ccccgcgacc gcgacgtcca ttccccattc ctcagggctg
      661 agatcttcgc tcagccagcc ggagaccgtg tcgacgatgg tgagcacgcc gaactcgtgc
      721 gcgatgcgac cgatctcctt catcggattc accgtccccg atggcgtctc cgagtgaacg
      781 accgagaggt acttgatccc cggctgggtc tgcaggacgc agcggacgtc ctccggatcg
      841 atcgccgcgt tgtacggaac ggccagctcg atcggctcgc cgccatactt gcggatccag
      901 tcctcgaaga acttcccgaa gactccggag accagattca ggaccttgtc gccgggctcg
      961 atcagcgagg cggctgccgc ctcgagaccg aggaccgcct ccgcctgcat gatcacggtg
     1021 tcgtagtcgg tccggaatgc cgccttcagc aaccggttgg cgttctcgaa gatctcgatg
     1081 aagcgcgggt cgtagtgata cagcgcggga cgttgcatcg cgcgcaatgt ctccggcgcg
     1141 atatcgaccg gtcccgatgc catggtgaaa cgtggccagt tcgtcatcct gattttctcc
     1201 ctgcatgtca cgacggcgcc ttcgatgcgc cgcgaggatc gctcacggag cgcattatcc
     1261 cccacgtctt cacgaaatct ggcagaatcg tgctcagcta ccgaaatcac cgtcgcaagg
     1321 agcccgacgc atgccacgac tcgccgcgaa tctcacgatg ctcttcaccg aagtcccctt
     1381 cctcgagcgc ttcgaccacg ccgccgccgc cgggttcaag ggtgtggagt tcctcttccc
     1441 ctattcggag acgccggagg cgatccagga cgcgctgacg aagaacggcc tcgagctggt
     1501 gctcttcaac ctgccc
//
LOCUS       DASTMD010000468         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1115536, whole genome shotgun sequence.
ACCESSION   DASTMD010000468 DASTMD010000000
VERSION     DASTMD010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115536"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFQ54_06800"
     CDS             complement(<1..297)
                     /locus_tag="VFQ54_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164737.1"
                     /translation="MVVDNAAETRDWIAELGVRPVINAAATLTKLGGSVMPPEVVAAM
                     SQASLAFVDYVELQKAVGIRIAALTRNDAAYVSSGAAAGITMTVAAILTEGLPER"
     gene            complement(288..1214)
                     /locus_tag="VFQ54_06805"
     CDS             complement(288..1214)
                     /locus_tag="VFQ54_06805"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0164738.1"
                     /translation="MTDSTTTGTTPETVATWWDAIDTELAAMPAAPERFAIPLYSTGF
                     ATTYQVRLTSIGRYRIAAYVSIPHGEGPFPALLAVPGYGSVVTPPQYEDRQRYVVMTL
                     MYRGTRHADWPYAGKFPGVLTDGIADSRTWVYRGILADHLRGLEFLAGMPEVDPSRIG
                     LIGNDIALLIAARRPSVTAVAVTASFFHQAWEVAQGSEAYPIEEINDYVRTCPADRDG
                     VRTSLALVDPGTHAAAISARVLLSVNEPGSGGDSAWWASLTANLGGQLEEYPVTHAGQ
                     ADRDAVDAWLATALGSEPKPRIWTPEELGPWS"
     gene            complement(1227..2207)
                     /locus_tag="VFQ54_06810"
     CDS             complement(1227..2207)
                     /locus_tag="VFQ54_06810"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU0164739.1"
                     /translation="MPQQRGRADQHQTSVVRPPDFDAFWADVQAELDATPLDPTLEPV
                     PMRSTPEVEVFEIGYTSLDGVRIAGWYCRPRESYIAGPYPGLSIVPGYVSEPTLPKSW
                     AKLGYAAIGVAPRGKLRSNRQFNPGYPGLLIHNIVDRNTYSYRAFYADAARAIDFLVA
                     RPEVIADRIGVHGSSQGGALTLTTAAMRPQNVTCGAAGAPYLTGFMDAAHLTHSYPYE
                     EMNEYLRLHPEREAAMRETVVYFDCINFAPSIEAPMLVYAGENDDVCPPETAHALVAA
                     MPGEVTLHVHPRCGHDAGAYWEMPVVEAFLAQHLQPNTPQANLRAQTTVG"
     gene            complement(2524..>3130)
                     /locus_tag="VFQ54_06815"
     CDS             complement(2524..>3130)
                     /locus_tag="VFQ54_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164740.1"
                     /translation="IASVAQGTPQSDGKYIADTSAQVLDQYADFCDNHGILLFLDLQF
                     GFRTVAEDTAGLEPWLRKPFVHLALDPEFHLTTPGEVPGQDLGAIDGSDVTWAQKYLV
                     DLSKKYDLPPKVLIVHQFNYYSITNKDTIKRVPGVQLVIDEDGFGSPDLKKNTYDVII
                     TQHPIEFNGIKLFYDPERDDPLMTPAEVIKLDPSPDLIVYQ"
BASE COUNT          528 a          932 c         1101 g          569 t
ORIGIN      
        1 ccgctcgggg aggccttcgg tcaggatcgc ggccaccgtc atggtgatgc ctgccgcggc
       61 acccgaggag acatacgcgg cgtcattccg ggtcagcgcc gcgatccgga tgccaaccgc
      121 cttctgcagc tcgacgtaat cgacgaaggc gagtgacgcc tgcgacatcg cggcgacgac
      181 ctccggcggc atcacggagc caccgagttt cgtcagcgtc gcggccgcgt tgatcacggg
      241 tcgaacgccg agctcggcga tccagtcgcg ggtttccgca gcgttgtcta cgaccatggt
      301 ccaagttcct ccggtgtcca gatgcggggt ttcggttcgc tgccaagggc ggtcgcgagc
      361 catgcatcga cggcatcgcg atcggcctga ccggcgtggg tgacaggata ttcctcgagc
      421 tgtccgccga ggttcgcggt cagggacgcc caccaggcgg agtcgccacc cgatccgggc
      481 tcgttgaccg agagcagcac cctcgcgctg atcgccgcgg catgggtgcc gggatcgacc
      541 agtgcgagcg aggtgcggac gccatcgcgg tcggcgggac aggttcgcac gtagtcgttg
      601 atctcctcga tcggataggc ctcggatccc tgcgcgacct cccacgcctg gtggaagaag
      661 ctcgccgtca ccgcaacggc cgtcacgctt ggtcgccgcg cggcgatcag gagtgcgatg
      721 tcgttgccga tcagcccgat tcggcttggg tcgacctcgg gcatgccggc gagaaactcg
      781 agacctcgca ggtggtcggc gaggatgccg cgatagaccc acgtccgcga atcggcgatg
      841 ccatcggtga ggacgccggg aaacttgccg gcgtagggcc aatcggcatg acgggtgccg
      901 cgatacatga gggtcatcac gacgtaccgc tggcgatcct cgtattgcgg tggcgtgacg
      961 acgctgccgt aaccggggac cgcgaggagc gccgggaagg gaccttcacc gtgagggatg
     1021 ctgacgtagg cggcgatccg gtagcgcccg atgctcgtca ggcgaacctg atacgtcgtc
     1081 gcgaacccgg tcgagtagag cggaatcgcg aatcgctccg gtgcggccgg catcgccgcg
     1141 agttcagtgt cgatcgcatc ccaccaggtg gcgacggtct ccggggtagt cccggtcgtg
     1201 gttgagtcgg tcacgatggc tcctccctac ccgacggtcg tctgggcgcg caggttggcc
     1261 tgcggcgtgt tcggctggag atgttgcgcg aggaacgcct cgacgaccgg catttcccag
     1321 tacgcgccgg cgtcgtgacc gcagcgcggg tgcacgtgga gcgtgacctc gcccggcatc
     1381 gccgcgacca gcgcgtgggc ggtctccggc gggcagacat cgtcgttctc accggcgtag
     1441 acgagcatcg gcgcctcgat cgaaggtgca aagttgatgc agtcgaagta gacgaccgtc
     1501 tcgcgcattg ccgcttcccg ttccggatgg agacgcaggt actcgttcat ctcctcgtag
     1561 ggataggagt gggtgagatg ggcggcgtcc atgaagccgg tcaggtaggg agcgcccgcc
     1621 gcgccacagg tgacgttctg cggtcgcatc gccgcggtcg tcagtgtcag cgcgccgccc
     1681 tgactcgagc cgtggacgcc gatcctgtcg gcgatcacct ccggccgggc gacgaggaag
     1741 tcgatcgcac gggcggcatc ggcgtagaac gcgcgatagc tgtaggtgtt gcggtcgacg
     1801 atgttgtgga tgaggagacc gggataaccg ggattgaact gccggttgga gcgcagcttg
     1861 ccgcgtgggg cgacgccaat cgcggcatag ccgagcttcg cccacgattt tggcaatgtc
     1921 ggttccgaga cgtagccggg gacgatgctg agcccgggat agggtcccgc gatgtacgac
     1981 tcgcggggac ggcagtacca gccggcgatg cggacgccgt cgagactcgt gtagccgatc
     2041 tcgaagacct cgacctccgg ggtcgaacgc atcggcaccg gctccagcgt cggatcgagc
     2101 ggcgtcgcgt cgagctccgc ctggacatcg gcccagaatg catcgaaatc gggtggtcgc
     2161 accacgctcg tctggtgctg atcggcacgg ccacgttgtt ggggcacggg gtcatccttt
     2221 cggtccgctg gtcgggagat tgcgccatta tctgacgtca ctatccggcg agaccgcgct
     2281 gatgacaaga gtccaggcgg gacactgccg tcccggatct tcgatttcat caacgtccaa
     2341 ggcgggacac ggaggtcccg ccgtacgaac ggccgctggg ggtgacgcgt ctgttcgtag
     2401 ggtgggacac gggaggtccc accgtacgaa cggccgctgg gggtgacgcg tctgttcgta
     2461 gggtgggaca cgggaggtcc caccgttggc tcttcatgga gcaggaatag agcgcagaac
     2521 acgtcattga tagacgatga gatcggggga agggtcgagt ttgatgacct ctgccggggt
     2581 catcagcggg tcatctcgct ccgggtcata gaagagcttg attccgttga actcgatggg
     2641 gtgctgcgtg atgatcacat cgtaggtgtt cttcttcagg tctggcgagc cgaagccatc
     2701 ctcatcgatc accagttgca cgcccggcac gcgtttgatc gtgtccttgt tggtgatcga
     2761 gtaatagttg aattgatgga cgatcaggac cttgggtggc aggtcgtatt tcttgctcag
     2821 atcgaccagg tacttctgcg cccaggtcac atcgctgccg tcgatcgcgc caagatcctg
     2881 tccgggcacc tcgcccgggg tcgtcaggtg gaattcggga tcgagtgcga gatgaacgaa
     2941 gggcttcctg agccagggtt ctagcccggc ggtatcctcc gccacggtgc gaaaaccgaa
     3001 ctggagatcg aggaagagca ggatgccgtg attgtcgcag aagtcggcgt attgatcgag
     3061 aacctgggcg gacgtatcgg cgatgtattt gccatccgat tgcggcgtgc cctgggcgac
     3121 ggacgcgatc
//
LOCUS       DASTMD010000469         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1831117, whole genome shotgun sequence.
ACCESSION   DASTMD010000469 DASTMD010000000
VERSION     DASTMD010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831117"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFQ54_06820"
     CDS             <1..322
                     /locus_tag="VFQ54_06820"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEU0164741.1"
                     /translation="AGTVLIVGALTYFPAYALGPIVEQKQMSSGVTFQMSDDGGGAYE
                     VVATPVAAAPAPPPAAASAPEAQPTAPAGNDAAGSTGTSSSSQSSQSSDQPAAVVDAS
                     SGGK"
     gene            481..2592
                     /gene="kdpB"
                     /locus_tag="VFQ54_06825"
     CDS             481..2592
                     /gene="kdpB"
                     /locus_tag="VFQ54_06825"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEU0164742.1"
                     /translation="MLPREERASSSGSPSKSPRRNLFDPEIAGPALKDAFRKLDPRNM
                     AKNPVMLLVEVGAAITTIILVLDLIGAADSFGLAIEHGQRWFVAWITVWLWFTVIFAN
                     FAEAMAEGRGKAQADTLRKTRSETMAKVITSGGGIESRASNALHLGDLVLVEAGDIIP
                     SDGEVVEGIAFVSEAAITGESAPVLKEPGTDTSSSVTGGTQVVSDHLRVRITAEPGNT
                     FLDRMIALVEGASRQKTPNEIALNILLAGLTIVFLITVITLGPIASYAKADVSITVLI
                     ALLVTLIPTTIGALLSAIGIAGMDRLVQHNVLAMSGRAVEAAGDVDVLLLDKTGTITY
                     GNRMASEFIPAPGVSIAELTNAALRSSLADETPEGKSIVALAGQQGYSIDRASLAGAE
                     LIPFTAETRMSGVNINGVATMKGAIDAIRRAATTTVPNEMIEASNRIAEQGATPLGVI
                     DNGRPLGVIALKDTVKPGMKERFEELRRMGIRTIMITGDNPLTAKSIAAEAGVDDFVA
                     EAKPEDKIRLIREQQAEGRLVAMTGDGTNDAPALAQADVGVAMNTGTAAAKEAGNMVD
                     LDSDPTKLISVVAIGKQLLMTRGSLTTFSIANDVAKYFAIIPAIFATAYGVPSSQGLG
                     FLNIMDLTSPQSAILSAIIFNALIIVALIPLALRGVAYKAMSAASLLQRNILIYGVGG
                     IITPFIGIKLVDIVLTAVGLT"
BASE COUNT          509 a          878 c          886 g          463 t
ORIGIN      
        1 cgccggtacg gtcctcatcg tcggtgctct gacctacttc ccggcctatg cgttgggtcc
       61 gatcgtcgag cagaagcaga tgagttccgg tgtcaccttc cagatgagtg acgacggcgg
      121 cggcgcctac gaggtcgtgg cgactccggt cgcggcggct cccgccccgc cacccgccgc
      181 agcgtccgca cccgaggcgc agcctaccgc gccagcgggg aacgacgcgg cgggctcaac
      241 cggaacatca tcgtcatcgc agtcatcgca gtcatccgat caaccggcgg ccgtcgttga
      301 cgccagctcg ggcggaaagt aggtcagctg gagatggcta ccaatccatt tcgattgaaa
      361 aaggacacct cgatgttccg gtctctggaa caggcattca ttcaaaccgg tgagagtccg
      421 gcggcggaag atcgggccca gacgggccgc gccgatacaa gccgtggggc gggagcaaac
      481 atgctcccgc gcgaggaacg tgcgtcatcg agcggctctc cgagcaagtc gccgcggcgg
      541 aatctcttcg atcccgagat cgcgggaccg gcgctgaagg acgcgttcag gaagctcgat
      601 ccgcgcaaca tggcgaaaaa cccggtcatg ctgctggtcg aggttggcgc ggcgatcacg
      661 acgatcatcc tcgttctcga cctgatcggc gccgccgatt cgttcgggct cgcgatcgaa
      721 cacgggcagc gctggttcgt tgcctggatc acggtctggt tgtggttcac cgtcatcttc
      781 gcgaacttcg cggaggcgat ggcggaagga cgcggcaagg cccaggctga taccctgcgc
      841 aagacccgca gcgaaacgat ggcgaaagtc atcacgagcg gcggcgggat cgagtcccgg
      901 gcatcgaacg ccctgcacct cggggacctc gtcctcgtcg aggcgggcga catcattccg
      961 agcgatggcg aagtggtcga aggtatcgcc ttcgtctccg aagccgcgat cacgggtgag
     1021 tcggcgccgg tgctcaagga gccaggaacg gatacctcgt cctcggtgac cggtggcacg
     1081 caggtcgttt ccgatcatct cagggtccgg atcacggcgg agcctggcaa tacctttctc
     1141 gatcgcatga tcgcgctggt cgaaggcgct tcccgccaga agactcccaa cgagatcgcg
     1201 ctgaacatcc tgctcgcggg tctcaccatc gtcttcctga tcaccgtgat cacgctcggc
     1261 ccgatcgcga gctacgcgaa ggcggatgtc tcgatcacgg tcctgatcgc gctgcttgtg
     1321 acgctgatcc cgacgacgat cggtgcactg ctctccgcga tcggtatcgc gggcatggat
     1381 cgcctggtcc agcacaacgt gctggcgatg agcggtcgcg ccgtcgaggc ggcgggtgac
     1441 gtcgatgtcc tgcttctcga caagacgggg acgatcacct acggcaatcg catggcgagc
     1501 gagttcatcc cggcgccggg ggtctcgatc gccgagctga cgaatgcggc cctgcgctcc
     1561 tccctcgcgg acgagacgcc ggaaggcaag tcgatcgtcg cgctcgcggg gcagcaggga
     1621 tattcgatcg atcgcgcgtc gctggccggc gccgaattga tccccttcac cgccgagacc
     1681 cgcatgagcg gtgtcaacat caacggcgtg gcgacgatga agggcgcgat cgatgcgatc
     1741 cgccgcgccg ccacgacgac agtcccgaac gagatgatcg aggcgtcgaa ccggatcgcc
     1801 gaacagggcg cgacgccact cggtgtgatc gacaacggac gtccgctcgg cgtgatcgcc
     1861 ctcaaggaca ccgtcaagcc gggcatgaag gagcggttcg aggaactgcg gcggatgggt
     1921 atccgcacga tcatgatcac gggtgacaac ccgctgacgg cgaagtcgat cgcggcggaa
     1981 gccggtgtcg acgacttcgt cgccgaggca aagccggaag acaagatccg cctcattcgc
     2041 gagcagcagg cggaaggtcg cctcgttgcg atgacgggag acggcacgaa cgacgcgccg
     2101 gcgttggccc aggccgacgt tggagtggcg atgaacacgg gcacggcggc ggcgaaggaa
     2161 gccggcaaca tggtcgatct cgattccgat ccgaccaagc tgatctccgt cgtcgcgatc
     2221 ggcaagcagt tgctgatgac gcgcggctcg ctgacaacct tcagcatcgc gaacgatgtc
     2281 gcgaaatact tcgcgatcat cccggcgatc ttcgccaccg cgtatggcgt gccctcgtcg
     2341 caggggctcg ggttcctgaa catcatggac ctgacgagcc cgcagagcgc gattctctcg
     2401 gcgatcatct tcaacgccct gatcatcgtc gcgctgatcc cgctggccct gcgcggcgtc
     2461 gcctacaagg cgatgagcgc ggcgtcgctg ctgcagcgga acatcctcat ctacggtgtc
     2521 ggcgggatca tcaccccgtt catcgggatc aagctcgtcg atatcgtcct gaccgcagtc
     2581 ggtcttacgt agtacccggg gcgggagcgt tcctcccgcc cacccaccac ccatgctgat
     2641 tcgtgggact cctgaaagaa ggattctgat gctcaaacaa ctccggaccg cgatcatcat
     2701 gatcgtggtg atgaccgcca tcacgggtat cgccta
//
LOCUS       DASTMD010000470         1570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_848312, whole genome shotgun sequence.
ACCESSION   DASTMD010000470 DASTMD010000000
VERSION     DASTMD010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848312"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFQ54_06830"
     CDS             <1..825
                     /locus_tag="VFQ54_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEU0164743.1"
                     /translation="PDILRRKLAIELDGKNQQLARLYGDAELDFNEIYQNLLRWCDEL
                     RPFVTATEVLVQDELALGRKILIECAQGAMLDIDYGSYPYVTSSSPTAAGACQGAGVA
                     PKQIDRVVAVYKAYSTRVGGGPMPAELEDEVGQTIRERGNEYGTTTGRPRRTGWFDAV
                     AARHTSRLNGVTDVAVTLVDVLDIFEEIKWCSAYRIGAEELHRVPALVDDLVDAVPVW
                     SSLPGWQTDTTGIRTFSDLPSELKSYLFELETILSTRIRYVGVGPERAQLIDRGAE"
     gene            complement(835..>1570)
                     /locus_tag="VFQ54_06835"
     CDS             complement(835..>1570)
                     /locus_tag="VFQ54_06835"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU0164744.1"
                     /translation="RAFGESAGLHLEGKLIVSAAKGIENETASRPSQILAEIRGRNDV
                     VVLSGPNLATEIASGLPAAAVVAGYDAGSARRAQRLLHSSSFRVYTSSDVVGVELGGA
                     LKNVIAIAAGISDGLQLGDNAKSSMMTRGIAEIARLGVRCGAERETFHGLSGFGDLIA
                     TCGSALSRNYRVGFALAQGKTLVEILENQVETAEGIPTTRSAMQLALREGIEMPITTQ
                     VSRVLFEDVSPREAIQALMGREATFE"
BASE COUNT          287 a          500 c          503 g          280 t
ORIGIN      
        1 cccgacatcc tccggcgcaa gctcgcgatc gaactcgacg gcaagaacca gcaactggcc
       61 cggctctatg gcgacgcaga gctcgacttc aacgagatct atcagaacct gttgcggtgg
      121 tgcgatgaac ttcgaccctt cgtgacagcc accgaggtgc tcgtccagga tgaactcgcg
      181 ctcggacgca agatcctgat cgagtgcgcc cagggcgcaa tgctcgacat cgactacggc
      241 tcatatcctt atgtcacgtc gtcgtcaccg accgccgcgg gcgcctgcca gggcgccggg
      301 gtcgcgccga agcagatcga tcgtgttgtt gcggtctaca aggcatacag cacacgggtc
      361 ggcggcggcc cgatgccggc cgagctcgaa gatgaggttg gccagacgat ccgcgaacgt
      421 ggcaacgaat acggaaccac gaccggtcga ccgcgacgca ctggctggtt cgatgctgtc
      481 gcggctcgac acacgagtcg actgaacggg gtgaccgatg tcgccgttac gctcgtcgat
      541 gtcctcgaca tcttcgagga gatcaagtgg tgctcggcat atcgcatcgg cgcggaagag
      601 cttcatcgcg tgccggcgct ggtcgatgac ctcgtcgacg cggtaccggt ctggtcgagt
      661 cttcccgggt ggcagacgga cacgaccggg atcaggacct tttccgatct tccgagcgag
      721 ctgaagagct atctgttcga actcgagacg attctttcga cgcgcatccg ctatgtgggt
      781 gtcgggcccg agcgcgccca gctcatcgat cgcggcgcgg aatagcggct ccagtcactc
      841 gaaggtggct tcgcgcccca tcagtgcctg gatcgcctcg cgcggggaga catcctcgaa
      901 gagaacccgg ctcacctggg tcgtgatcgg catttcgatg ccttcgcgca gggcgagttg
      961 catcgccgaa cgcgtcgtcg ggatcccttc ggctgtctcg acctggttct ccaggatctc
     1021 gacgagcgtc ttgccctggg ccagggcgaa gccaacacga tagttccgcg agagtgcgct
     1081 gccgcaggtc gcgatcaggt cgccgaatcc cgaaagcccg tgaaacgtct cgcgttcggc
     1141 gccgcatcgc acgccaaggc gggcgatctc ggcaatgcca cgcgtcatca tcgatgattt
     1201 cgcgttgtca ccaagctgaa gtccgtcgct gattcccgcg gcgatcgcga tcacgttctt
     1261 caatgcgccg ccgagttcga cgccaacgac atcggacgac gtgtagacgc ggaacgacga
     1321 cgagtgaagg agccgctgcg cgcgtcgcgc cgatccggcg tcgtagccgg caacaacggc
     1381 ggcggccggg agcccggagg cgatttcggt tgccaggttg ggacccgaga gcacgacgac
     1441 atcgttgcga ccacggattt cggcgaggat ctgggacgga cgcgacgcgg tttcgttctc
     1501 gattcctttt gccgccgaga cgatcagctt gccctccaga tggaggccag ccgattcgcc
     1561 gaacgcacgc
//
LOCUS       DASTMD010000471         5118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3079153, whole genome shotgun sequence.
ACCESSION   DASTMD010000471 DASTMD010000000
VERSION     DASTMD010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3079153"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1544)
                     /locus_tag="VFQ54_06840"
     CDS             complement(111..1544)
                     /locus_tag="VFQ54_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0164745.1"
                     /translation="MAVQATDRHQENVIEWLDERESRFVGIANQIWEKPETALTEVFA
                     SKLQADDLAADGFTITTNIADLPTAFMAEWSNGTGGPTFGFMGEYDALPGLSQERSPT
                     QSAIVANGPGHGCGHNLLGSAALAATAVVKAWMQATGTAGTVRYYGCPAEETVEGKNF
                     MARAGAFDDLDFAVSWHPGSVNTPSGGTSLASDNIKFRFRGRTAHAAGNPETGRSALD
                     AVELMNIGVNFLREHTIDQARLHYVITNGGGAPNVVPDDCEVWYFIRSPERHQVNELT
                     ERVRNIARGAALMTETRMEETFISGIYNLLPNKVMVDRMEENLRAIGPIDFTPEEKAF
                     AQEIASAYPLETRESILKAEHLPASLASEGITGEIYPTLDFNQLMMGGTDVADISWVT
                     PTVQMTSACFPLGVPGHSWANTATAGMSIGHKGLIFAAKAMALTAADFVLDPDLLQAA
                     KDEFAASTKGRPYVSPIPASVHPRTKK"
     gene            complement(1610..3040)
                     /locus_tag="VFQ54_06845"
     CDS             complement(1610..3040)
                     /locus_tag="VFQ54_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0164746.1"
                     /translation="MATETPGKTHENVVEWLEERQGRFITIADTIWKNPELALAEYKA
                     CKLQSEDLAADGFTITGNIGDLPTAFMAEWTSGTGGPIIGFLGEYDALPGLSQDIVSA
                     QSPLVVDGPGQGCGHNLLGTAALAAASVVKAWLQATGTPATVRYYGCPAEETCEGKVY
                     MARAGAFDDLDMAITWHPGSVNSVWAGSSLAVNNIKFRFKGRTAHAAANPETGRSALD
                     AVELMNVGVNYLREHMPDAARIHYVITNGGGAPNVVPDDCEVWYFVRSPERAQVDELT
                     ERVRNIAKGAALMTETTMTENFLSGAYNMLTNMTLSDQMIEVLEELGPLEFTEEEITY
                     AKTIAGAFPEELRRGILKSEHLPESMISAGLDGNVWPIRDAGQVMPGSTDVSDVSWIT
                     PTSQITTACFPLAVPGHSWAITATSGNSIGHKGMIHAAKAMAMVASDLITDPALLQKA
                     KDEFTAATAGRPYQCPIPADVQPRRP"
     gene            complement(3298..3708)
                     /locus_tag="VFQ54_06850"
     CDS             complement(3298..3708)
                     /locus_tag="VFQ54_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0164747.1"
                     /translation="MDLNDAGQLVTYVWLETLRHFPAVTLDAFVMMPDHMHAILTIGD
                     GSAPASMPTLGNVVQRFKSLTTVLYGKGVRLGIYPPYETRLWQFRYHDHIIRDTDDLA
                     SHRRYIESNPLRWAERMATDGPPSLTGDPVNRGM"
     gene            complement(4063..4863)
                     /locus_tag="VFQ54_06855"
     CDS             complement(4063..4863)
                     /locus_tag="VFQ54_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEU0164748.1"
                     /translation="MKIPFYQIDAFTNRAFSGCPAAVCPLDAWLPDDLLQKIANENNL
                     SETAFFVPDSGDHDYHIRWFTPTVEMDLCGHATLASAALIFRDLRPELDTIRFSSLSG
                     TLVVNRAGGGRFELDFPLRAGEPGEVPDELASALGARPDEVRVAGWSMAVFKDAGTIR
                     TVTPDMGLLADRFPAGVMVTAPGDVDGIDYVVRVFAPGIGIPEDPATGSAQCTLVPYW
                     ADRLGKTELRALQVSARGGEFRLRLDGATNRALIGGGAVFVIEGTLTI"
BASE COUNT          892 a         1547 c         1709 g          970 t
ORIGIN      
        1 cgtgcgtggt agatgtggta gccgaagcca tgtgagtccc tcctgaatac gtgagacctg
       61 agatcgggca cgtcgtggcc gtcgtcgcgg cgtggaagaa acgcgtcttc ctacttcttc
      121 gtgcgcggat ggacgcttgc cgggatcggg ctcacgtatg gtcgaccctt cgtcgacgcg
      181 gcgaattcgt ccttcgcggc ctggagaaga tccggatcga ggacgaagtc ggcggcggtg
      241 agcgccatcg ccttcgcggc gaaaatgaga cccttgtggc cgatcgacat ccccgcggtt
      301 gcggtgtttg cccagctgtg acctggcacg ccgagcggga agcaggcgct ggtcatctgg
      361 acagtcggtg tgacccacga gatgtcggcg acatcggtgc cacccatcat cagctggttg
      421 aaatcgagcg tgggatagat ctcgccggtg atcccttcac ttgcgagcga cgccgggaga
      481 tgctccgcct tcaggatcga ctcccgcgtc tcgagcgggt aggcggaagc gatctcctgc
      541 gcgaacgcct tctcctccgg cgtgaagtcg atcggcccga tcgcgcggag gttctcctcc
      601 atcctgtcga ccatcacctt gttcggcagg aggttgtaga tgccggagat gaaggtctcc
      661 tccatccggg tctcggtcat cagcgccgcg ccgcgcgcga tgttgcgcac gcgttccgtc
      721 agttcattca cctggtgccg ctctggggag cggatgaaat accagacctc gcagtcatcc
      781 ggcacgacgt tcggggcgcc gccgccattc gtgatcacgt aatgcagtcg cgcctggtcg
      841 atggtgtgtt cgcggaggaa gttgacgccg atattcatca gctcgacagc atccagcgcc
      901 gagcgaccgg tctccggatt gccggcggcg tgggcggtgc gaccgcggaa gcggaacttg
      961 atgttgtcgg aggcgaggct ggtgccacca cttggcgtgt tcaccgaacc aggatgccaa
     1021 ctgacggcga aatcgagatc gtcgaaggcg ccggcccggg ccatgaagtt cttgccctcg
     1081 accgtctctt cggccgggca gccgtagtag cggacggtgc ctgcggtgcc ggttgcctgc
     1141 atccaggcct tcacgacggc ggtcgcggcg agcgccgccg aaccgaggag attatgcccg
     1201 cagccgtgcc cgggtccgtt cgccacgatc gcggactgtg tcggcgatcg ttcctgggag
     1261 agaccgggaa gggcatcgta ttcgcccatg aaaccgaagg tcggaccacc ggtgccattg
     1321 gaccactccg ccatgaatgc ggtcggcaga tcggcgatgt tcgtggtgat cgtgaatccg
     1381 tcggcagcga gatcgtcggc ctgcagtttc gaggcgaaga cctcggtcaa cgccgtctct
     1441 ggcttctccc agatctggtt cgcgatgccg acgaagcggc tctcgcgctc gtcgagccat
     1501 tcgatcacgt tttcctgatg acggtcggtt gcctgaacgg ccatgcaggt cctttctcgt
     1561 gggtatgaga agaccgcccc acgatgcagg gcggtccgca tcgagacggc tacgggcggc
     1621 gcggctggac gtcggccggg atggggcact ggtacggtcg accggcggtc gcggcggtga
     1681 actcgtcctt cgccttctgg agcagcgcgg gatcggtgat caagtcggag gcgaccatcg
     1741 ccatcgcctt cgcggcgtgg atcatcccct tgtgcccgat cgagttaccg ctcgtcgcgg
     1801 tgatcgccca ggagtgaccg ggaacggcga gcgggaagca ggccgtcgtg atctgcgagg
     1861 tcggcgtaat ccaggaaacg tcggagacgt cggtcgaccc cggcatgacc tgaccggcat
     1921 cgcggatcgg ccagacgttg ccgtcgaggc cggcggagat catcgattcc ggcagatgct
     1981 cggacttgag gatgccgcgg cgcaactcct cggggaaggc tccggcgatc gtcttcgcgt
     2041 aggtgatctc ttcctcggtg aactcgagcg ggccgagctc ctcgaggacc tcgatcatct
     2101 gatcgctgag cgtcatgttc gtgagcatgt tgtaggcgcc ggagaggaaa ttctccgtca
     2161 tcgtcgtctc ggtcatcagc gccgcaccct tggcgatgtt gcgcacgcgc tcggtcagtt
     2221 cgtcgacctg ggcgcgctcg ggggagcgga cgaagtacca gacctcgcag tcgtccggca
     2281 cgacattcgg ggcgccgccg ccattcgtga tcacgtagtg gatgcgggcg gcatcgggca
     2341 tgtgttcgcg gaggtagttg acgccgacgt tcatcaattc cacggcgtcg agcgccgaac
     2401 gacccgtctc cgggttcgcc gccgcgtggg cggtgcggcc cttgaagcgg aacttgatgt
     2461 tgttcacggc gaggctgctg ccggcccaga ccgaattcac ggaaccgggg tgccaggtga
     2521 tcgccatgtc gaggtcgtcg aaggcgccgg cgcgggccat gtagaccttg ccttcgcagg
     2581 tttcttccgc gggacagccg tagtaacgaa cggttgccgg cgtgccggtc gcctggagcc
     2641 acgccttgac gacgctggcg gcggcgaggg ccgcggtgcc gaggaggttg tgaccgcagc
     2701 cctggccggg gccatcgacc acaagcggcg actgggcgct gacgatgtcc tgcgacaagc
     2761 ccggcagggc gtcgtactcg ccgaggaagc cgatgatcgg accgccggtg ccggaggtcc
     2821 attcggccat gaaggccgtc gggagatcac cgatgttgcc ggtgatcgtg aagccatcgg
     2881 cggcgaggtc ttccgactgg agcttgcagg ccttgtactc ggcgagcgcg agttccggat
     2941 tcttccagat cgtatccgcg atcgtgatga atcgcccttg tcgctcctcg agccattcga
     3001 ccacgttctc gtgcgtcttg cccggtgtct ccgtcgccat gtggcagcct ctccatggtg
     3061 tgttggtacg tcgcgaagat tggtcccgcc tgcgcggtga tgagcacatt gtgtgcagct
     3121 tcgctgaaat gggggcgcgg tgcaatggtc gggaaggtgg ggattgcaca aatcggttcg
     3181 cacggcgaac gcgcttgtgc cagttgcgga gccgaacgtc tactcagttg tgcaatttgt
     3241 ggaaacaagc agacggcatg atgggacggc ccttgcggca agacacaagg tcttgcccta
     3301 cataccccgg ttcaccggat ctcccgtgag ggatggcgga ccgtccgtcg ccatgcgctc
     3361 ggcccaacgg agaggattgg actcgatgta cctgcgatga ctcgccagat cgtccgtgtc
     3421 ccgaataatg tgatcgtgat agcggaattg ccagagccgt gtctcgtatg gcggatagat
     3481 gccaaggcga actcccttgc cgtagaggac ggttgtcagg ctcttgaatc gttggacgac
     3541 attgccaagg gtcggcattg aggcaggggc cgagccatcc ccgatcgtca ggatcgcgtg
     3601 catgtgatcc ggcatcatga caaaagcatc cagcgttacg gcagggaaat gtctcagcgt
     3661 ttcaagccag acatacgtga cgagctggcc cgcatcattc aggtccattc ctccgtcgct
     3721 ggaaatattg ccgaaccgcg gctcgcggat ggcaagcgca atcgtaatga agtacggacc
     3781 cggcaatctg tagtcgtgtt cgcggatgcg aatctgcttt cgagtcggcc ggaggttcac
     3841 gatgttcccc atcacgcgtt gcgttctgat ggcatgaccc cgaaacggtc atacgcgaat
     3901 gatgtcctcg cgaaaacccg ggtttgtagg gcaagacctt gtgtcttgcc gcgggaacca
     3961 acgcatcacg ccttgtcctc cgtcgaacgg cgtcacgcac gacccgggtt tgtagggcaa
     4021 gggcttgtcc cttgccgttg atccagcgct cggtcagccg gttcagatcg tcagcgttcc
     4081 ctcgatcacg aagacggcgc cgccgccgat cagggcgcgg ttcgtggctc catcgagccg
     4141 caaccggaac tccccgccgc gggcgctgac ctgcagcgca cggagttccg ttttccccag
     4201 gcgatccgcc cagtagggga cgagcgtgca ctgcgcggag ccagtcgccg gatcctccgg
     4261 gataccgatg cccggcgcga agacgcgcac gacgtagtcg atcccatcca cgtcgccggg
     4321 cgcggtgacc atcacgcccg ccggaaagcg atcggcgagt aatcccatat ccggggtcac
     4381 cgtccggatc gtgccggcgt ccttgaagac ggccatcgac catccggcga cgcgtacctc
     4441 gtcaggtcgc gcgccgagtg ccgaggcgag ttcatcgggg acttcacccg gttcgcccgc
     4501 acgtaggggg aagtcgagtt cgaaacggcc acccccggcg cgattcacga ccagcgttcc
     4561 actcaggctc gagaaccgga tcgtatccag ctcgggacgc agatcgcgga agatcagggc
     4621 tgccgacgcc agcgtcgcgt gaccgcagag atccatctcc accgtcggcg tgaaccaccg
     4681 gatgtgatag tcgtgatcgc cggaatcggg cacgaagaac gcggtctccg agaggttgtt
     4741 ctcgttcgcg atcttctgga gcaggtcgtc ggggagccag gcatcgagcg ggcaaaccgc
     4801 cgccgggcat ccactgaacg cgcgattggt gaaggcgtcg atctggtaga acgggatctt
     4861 catgggtctg gaagctccac tgcgcattgc gcgatccgaa ggatttcggg acgacactac
     4921 cagttcgtgc ggccatcgcg cgagtcgtga ccgaagcgaa tcgccgtgat atcgaggacg
     4981 atgaacgcga gcatgaagat cagaaacgcc acggtagtgg tcctttcatt gaggatgtga
     5041 ctcactcgag ccacatcgga atcagcgaga cgatgacgag tgatccgaga atcgtgttgg
     5101 cgacattcca gtggcgct
//
LOCUS       DASTMD010000472         4113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_580191, whole genome shotgun sequence.
ACCESSION   DASTMD010000472 DASTMD010000000
VERSION     DASTMD010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580191"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1000
                     /locus_tag="VFQ54_06860"
     CDS             59..1000
                     /locus_tag="VFQ54_06860"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEU0164749.1"
                     /translation="MREFARNLTESLVEAAAEAPSRQGYGGQSMVAPLKRVLVQTPAR
                     SLSPDDAAHFGYPHAPNDDLAMSEHSAFVTLLQEQGIEVIQQDAAPDGELDSIFVYDS
                     GYITDDGALLTRPGKVVRRGEPRYAASAFADLGISIIGEIEEPGTLEGGDMCWLDPQV
                     LAVGESYRTNANGIDQLGVIMQAVGVDLYRVPLPHWHGEDECLHLLSLISPVAEQMAV
                     VYLPLLPVPFVQTLRRSGWTLIETPDEEFASQGCNVLALAPRKVLMLKDNPITRTRLD
                     AAGVETIVYTGDEISHNRAGGPTCLTRPILRDMHDEG"
     gene            1163..1543
                     /locus_tag="VFQ54_06865"
     CDS             1163..1543
                     /locus_tag="VFQ54_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164750.1"
                     /translation="MSFNAYTPPGLWDRTRLARIGFVTGLIFGIGLGWFFHGVISLII
                     RFGILVLLLIPLMLIAWLWWRSSRTVYRRDGGGTVMTWTTANFPGFGSAPSDNAPRSD
                     DRRSQESGQVYDLDELKRQQERNR"
     gene            1558..2310
                     /locus_tag="VFQ54_06870"
     CDS             1558..2310
                     /locus_tag="VFQ54_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0164751.1"
                     /translation="MAVVCCAVGVLLGFVGAGGAGVMVSLLNLGFHLPIHEAVGTSLA
                     AMFFVTLSGTISHYREGNTNVRAGIMIGVAGIVGAVAGADVGQSIPEHILQPMAGFAL
                     WFLALLVYLRARISARATSPEFDPAHAVPTTRQNVGAVALGISGGVASAFFGVGMTPW
                     IQLGTLTILKLPLIKTIGTTMLALIFISLSGSVALARHGDVSVPHLIGATIGMTIGSY
                     VGAKFTRRAPLWLLRGAIVLTPFTAGTLLIFF"
     gene            2610..2921
                     /locus_tag="VFQ54_06875"
     CDS             2610..2921
                     /locus_tag="VFQ54_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU0164752.1"
                     /translation="MAVEFTKDDIYAGLKNVYDPEIGINIVDLGLVYDADIAETGDVM
                     ITMTLTSIGCPLGPIIVQEVDNALKDLPEIGKTDVKLVWSPAWSPDMMTEEAKDELGI
                     W"
     gene            3131..>4113
                     /locus_tag="VFQ54_06880"
     CDS             3131..>4113
                     /locus_tag="VFQ54_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0164753.1"
                     /translation="MRQQRRSTRRTSATSPRSRIGYTIRPAALLVVILSGIVGLFGIH
                     HVQSVDPAVDLSPTLAPPTVQVLPTRDATFVPPPTGIPPSEVPTIVPSLVPTATLVPT
                     IAPTAGPTEIPTPGPRPAAPATGMTSKSYILYRGESGRKEVALTFDAGQTTGYTSQML
                     DILDQYGIKGSFGVTGEWAQANPDLLKRMVDEGHMLINHTYDHASWSGLSLGTEPLTA
                     DQMTQELTKTEQIIKDQTGYELKPYFRFPYGDYNDLSLQVLFQNGYDWTLWWTCDTEA
                     WNGNVASQIEEKCSPDDPTHGGPGAIILMHVIQEEDLKALPALIDAYKAEG"
BASE COUNT          745 a         1292 c         1314 g          762 t
ORIGIN      
        1 atgctaagat ttcttccatt cgcaggtcac aggggattgg tcacgagagg agcgcgagat
       61 gcgggaattc gcacggaatc tgaccgaatc gctggtcgag gcggccgccg aggcgccatc
      121 gcgccagggg tatggcggtc aatcgatggt cgcgcccctg aagcgcgtgc tggtgcagac
      181 gccggcgcga tcgctctcgc cggacgatgc cgcgcatttt ggctatccgc acgccccgaa
      241 cgacgacctc gccatgagcg agcattcggc cttcgtcacc ttgctgcagg agcaggggat
      301 cgaggtgatc cagcaggatg ccgcgccgga cggcgaactc gattcgatct tcgtctacga
      361 ctccggttac atcacggacg acggcgcgct cctgacccgt cccggcaagg tcgttcgccg
      421 gggagagccg cgttatgccg cgtccgcctt cgccgatctc ggaatctcga tcattggcga
      481 gatcgaggag cccggtacgc tcgagggcgg cgacatgtgc tggctcgacc cccaggtgct
      541 cgccgtgggc gagagctacc gcaccaatgc caacggcatc gatcaactgg gcgtgatcat
      601 gcaggcggtt ggcgtcgatc tctatcgcgt gccccttccg cattggcatg gcgaggacga
      661 gtgcctgcat ctcctctcgc tgatcagccc ggtcgccgag cagatggcgg tcgtctatct
      721 tcccttgctc ccggttccgt tcgtgcagac gctgcgtcgc tccgggtgga cgttgatcga
      781 gaccccggac gaggagttcg cctcccaggg ctgcaacgtt ctcgccctcg cgccgcgcaa
      841 ggtgctcatg ctgaaggaca acccgatcac gcgaacgcga ctcgacgcgg cgggtgtcga
      901 gacgatcgtc tacacgggag acgagatctc gcacaatcgc gctggcggac cgacctgcct
      961 gacgcgtccg atcctgcggg acatgcatga tgaaggctga tcccgccgaa atgggggata
     1021 accagccgcc cacgggcgca gctttcggct aagcttgata tcagcaggag atcgacagaa
     1081 tcgacgctgc cctcctgcct gaattcggcg cccgaatctc tccaatggtg gatgttgacg
     1141 ggcgttttca gggtggtcct tcatgagctt caatgcatac acaccaccgg gactctggga
     1201 cagaacgcgt ctggcgcgca ttggcttcgt caccggtctg atcttcggga tcggcctcgg
     1261 ctggttcttt cacggcgtca tctccctgat cattcgcttc gggatcctgg tcctcctgct
     1321 gatcccgctg atgctgatcg cctggctctg gtggcgctcc tcgcgtaccg tctatcgtcg
     1381 cgatggcggc gggacggtga tgacgtggac gacggcgaac ttcccgggat tcgggagcgc
     1441 cccgtctgac aacgcgccgc gatccgacga ccggcgctcg caggagagtg gccaggtcta
     1501 cgacctcgat gaactgaagc ggcagcagga gcgaaatcgc tgacacttgt cctgaccttg
     1561 gccgtcgtct gttgcgccgt cggagtcctt cttggcttcg tcggcgccgg cggcgcagga
     1621 gtgatggtct cactcctcaa cctcggcttt catcttccga ttcacgaggc cgttggcaca
     1681 tcgctggcgg ccatgttttt cgtgaccctt tcgggcacga tctcccacta ccgcgagggc
     1741 aatacgaacg tgcgcgccgg gatcatgatc ggcgtcgccg ggatcgtcgg tgcggtggct
     1801 ggggctgatg tcgggcagtc gatccccgag cacatcctcc agccgatggc cggcttcgcg
     1861 ctctggttcc tcgccctcct cgtctatttg cgggcccgca tctcggctcg tgcgacctcg
     1921 cccgaattcg acccggccca cgccgtgccg acgacccgcc agaatgtcgg agcggtggcg
     1981 ctcggtatct ccggaggcgt ggcgtcggcg ttcttcgggg tgggaatgac gccgtggatc
     2041 cagctcggca cgctgacgat cctcaagctg ccgttgatca agacgatcgg gacgacgatg
     2101 ctggcgctga tcttcatcag cctcagcggc agcgtcgcgc tcgcgcggca cggtgatgtc
     2161 tccgtaccgc atctgatcgg cgcgacgatc gggatgacga tcggttcgta tgtcggggcg
     2221 aagttcacgc ggcgagcgcc gctctggttg ctgcggggcg cgatcgtgct gacgccgttc
     2281 acggccggaa ccttgctgat cttcttctag ctgtcccggc tcgatgaccg gatgctatcg
     2341 accggagaag gcggcctgca gccgcgggcg accaccaggg tcgcccctac gacgacgtca
     2401 tctggcatgc ccacgtaggg gtcagcctcg tgctgacccg cggccgcagg ccgcctcatg
     2461 gcttggactc acaattggta agcgaaaatt cccacgggaa tggtcggggt tcggtagaat
     2521 aggaagagca gattccgggg gcaggatgcc cgcggatctc ccggacgcgg atatgcgtgc
     2581 gaacgggatg ttcatggagg acacaatcga tggcggtgga attcaccaag gacgacatct
     2641 acgcgggtct gaagaacgtc tatgacccgg aaatcggcat caacatcgtc gacctcgggc
     2701 tggtctatga cgcggacatc gcggaaaccg gcgatgtcat gatcacgatg acgctgacct
     2761 cgatcggttg cccgctcggc ccgatcatcg tgcaggaagt cgacaacgcg ctcaaggatc
     2821 tgccggagat cggcaagacg gacgtcaagc ttgtctggag tccggcctgg tcgccggaca
     2881 tgatgacgga agaagccaag gacgaactcg gcatctggta acggattcca gtctccagat
     2941 gtgacgaccg gctccgcgag gggccggtcg ccgtattttg aaacgatgcg atgacggttc
     3001 attcgtggta ccgtgatgta cgtacgcgaa tagcggtcgc acgtcgtcca tggttcatcc
     3061 gggaagccgt gtggagcgta ggttccagcg aggctcgctc ggagcgaatg agaacgggac
     3121 tgatcggcag atgaggcagc agcgcaggag cacgagacgg acaagcgcga cttcgccgcg
     3181 aagtcgcatc ggctacacga ttcgtccggc ggcgctcctg gtggtgatcc tctccgggat
     3241 cgtcgggctc ttcggcatcc accatgtgca gtccgtcgat cccgcggtcg atctctcacc
     3301 gaccctcgcg ccgccgaccg ttcaggtcct gccgacacgg gatgcgacgt tcgtgccgcc
     3361 gccgaccggg attccgccat cggaagtccc gacgatcgtc cccagcctcg tgccaaccgc
     3421 aaccctcgtc cccaccatcg ctccaactgc gggaccaacc gagatcccga cacccggtcc
     3481 gcgtccggca gcgccggcaa cgggaatgac ctcgaaatcc tacattctct atcgcgggga
     3541 atcaggacgg aaggaagtcg cgctcacctt cgacgctggt cagacgacgg gttacacgtc
     3601 gcagatgctc gacatcctcg atcagtacgg gatcaagggc agcttcgggg tcaccggcga
     3661 gtgggcgcag gcaaatccgg atctcctcaa gcggatggtg gatgaggggc acatgctgat
     3721 caaccacacc tacgatcacg cctcctggtc cgggttgtcg ctcggaaccg aaccgctcac
     3781 ggcggaccag atgacgcagg aactgacgaa gacggaacag atcatcaagg accaaacggg
     3841 gtatgagttg aaaccatact tccgctttcc atacggagac tacaacgatc tctccctgca
     3901 ggtgctgttt cagaacggct atgactggac gctctggtgg acgtgcgaca ccgaggcgtg
     3961 gaacggcaac gtcgccagcc agatcgagga gaagtgctcg cccgacgatc cgacgcacgg
     4021 cggacccggg gcgatcatcc tgatgcacgt gatccaggaa gaggacctca aggcactccc
     4081 cgcgctgatc gatgcctaca aggcggaagg cta
//
LOCUS       DASTMD010000473         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_668554, whole genome shotgun sequence.
ACCESSION   DASTMD010000473 DASTMD010000000
VERSION     DASTMD010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668554"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..581
                     /locus_tag="VFQ54_06885"
     CDS             279..581
                     /locus_tag="VFQ54_06885"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0164754.1"
                     /translation="MTSISEAQAEEMAGWMRALADPTRIRILNMLARREDAVCVCEFV
                     DEFPLGQSTISHHLKILRDARFVFAERRGTFMYYQINHTELDALPGAVRHILNAGI"
     gene            663..2117
                     /locus_tag="VFQ54_06890"
     CDS             663..2117
                     /locus_tag="VFQ54_06890"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU0164755.1"
                     /translation="MDRLEYPVVVIGAGPVGLAAAAHLKSRAIEPLVIERGSQVAANV
                     AEWGHVRFFSPWRYSVDTAAAALLEAHGWIAPDPESYPTGADLVSRYLKPLAGLPEIQ
                     PQLRLQTEVVSVTREGFDKMKTPGREHAPFLVTTRDADGHEENVLARAVIDASGTWNH
                     PNPLGASGQFAIGERPLAEHIAYGIPDVLGAARTRYAGKRVLVVGSGHSAFNVLLDLV
                     DLADSAPGTSITWAIRREGERLGNLFGGGIGDALPARGQLGDRVRSLVEQGRLRLVTG
                     VRITRLVATAEGIVVSGQDSVLEPVDEIIAATGFRPDLGLLSELRLALDAAVESPTAL
                     APLIDPNVHSCGSVPPHGAEELKHPEPDLYIVGMKSYGRAPTFLMLTGYEQVRSVAAA
                     IAGDWEAARDVQLVLPETGVCSTSALTDRGVACCAPASEAASDLEASCCGGGGAIEQD
                     GGGSVQSGACCQSAAPQVIQLAAIGRARSGEKCC"
     gene            2446..>3069
                     /locus_tag="VFQ54_06895"
     CDS             2446..>3069
                     /locus_tag="VFQ54_06895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164756.1"
                     /translation="MKRFLGLYYGWRITAALAVTETISWGILYYAFSVFLVPMHDDLG
                     WSVTAMTGAYSLALLVSGLLAPLVGRWLDRRGPRGIMTAGSAVGVIALLTWSRVDSIA
                     GFYVLWVGIGIAMSTTLYEPAFATLTRWFSRGRSRALLIVTIAAGFASTIFLPFASLL
                     VEHLGWRHALLVLAAILGITAIPLHGFILRRRPEDLGLTVDGDPGSET"
BASE COUNT          484 a         1016 c         1018 g          551 t
ORIGIN      
        1 ggacctggct gacgggcacg catatcgttt cccgcgaact ggtaacgaca cgctgaccac
       61 atcgccggac atgaaaccct ggcaggatag acgcgacgga aaccacgata atgaagatgc
      121 tgcggcaatg gtccatcgat acagaccgat ggatatattg acaaccatcg atacggtgga
      181 tatgatgtcg gtagtagaag ggtgctttga tggtcgtcgc tcaacccatt gctcgcaaaa
      241 ccgttcccgt cgtcaacacc tgttgcgatc tcgacgcgat gaccagtatc tccgaagcgc
      301 aggcggaaga gatggcgggc tggatgcgtg cgctcgcgga tcccacccgc attcgcatcc
      361 tgaacatgct cgcccggcgc gaggacgcgg tctgcgtctg cgagttcgtc gacgagtttc
      421 cgttggggca gtcgacaatc tcccaccacc tcaagattct gcgcgatgcc cggttcgtct
      481 ttgccgagcg gcgcgggacc ttcatgtact accagatcaa tcacaccgaa ctcgacgcac
      541 tgccaggcgc cgtccgccat atcctgaacg ccggaatcta gacgcgaaga tacccatcga
      601 ggatttcgct gggtcgtcta tatcgaacat cgtcgatgct cgatgcaacg tgaggagcca
      661 tcatggatcg tctcgaatat cccgtcgtcg tgatcggtgc ggggccggtc ggactcgccg
      721 ccgcggcaca cctgaagtcg cgcgcaatcg agccgctcgt catcgagcgg ggatcgcagg
      781 tcgcggcgaa cgtcgcggaa tggggccacg tccgcttctt ttcaccgtgg cgatattcgg
      841 tcgatacggc cgctgccgcg cttctcgagg cgcacggctg gatcgccccc gatcccgaga
      901 gctatccgac gggtgcggat ctcgtgagcc gctacctcaa gccactggcc gggcttccgg
      961 agatccaacc gcaactccgg ctccagaccg aggtcgtttc cgtcacccgc gagggcttcg
     1021 acaagatgaa gacgccgggt cgcgagcacg caccgttcct cgtcaccacg cgcgatgccg
     1081 acggtcatga ggagaacgtc ctcgcccggg cggtgatcga tgcctccggg acgtggaatc
     1141 atccgaatcc actcggcgca tcggggcagt tcgcgattgg cgagcgtccg ctcgcggagc
     1201 acatcgccta cggcatcccg gacgtgctcg gcgccgcgcg cacgcgttat gccggcaagc
     1261 gcgttctcgt cgtcggcagc ggtcactcgg cgttcaacgt cctgctcgat ctcgtcgacc
     1321 tcgcggattc cgcacctggg acatcgatta cctgggcgat ccgccgcgaa ggcgagcggt
     1381 tggggaacct cttcggtggc gggataggcg acgccctccc cgcccgcggt cagctcggcg
     1441 atcgggtgcg gagtctcgtc gagcagggac ggctccggct cgtcaccggc gtccggatca
     1501 cgcggctcgt tgcgacggcg gaagggatcg tggtttcggg tcaggacagc gtcctcgagc
     1561 cggtcgacga gatcatcgcg gcaaccggct tccgaccgga cctcgggctg ctctccgagc
     1621 tccggctggc tctcgacgcc gccgtcgaga gcccgaccgc cctggcgccg ctgatcgatc
     1681 ccaacgtgca cagctgcggc tcggttcctc cccacggggc ggaggagctg aagcacccgg
     1741 agccggatct ctacatcgtc ggcatgaaga gctacgggcg cgccccgacc ttcctgatgc
     1801 tgaccggcta cgagcaggtg cgctcggtcg ccgcggcgat cgccggggac tgggaggcgg
     1861 cgcgtgacgt ccagctcgtg cttccggaga ctggcgtctg ctcgacgagc gcgctgacgg
     1921 atcggggcgt cgcctgttgc gcgccggcat cggaagcggc aagcgacctg gaggcgagct
     1981 gctgtggcgg tggcggtgcg atcgaacagg acggcggcgg ctcggtgcag tctggcgcct
     2041 gctgccaaag cgcggcgcca caggtcattc agctcgcggc gatcggtcgc gcgcgtagcg
     2101 gcgagaagtg ctgctagcgg agaggcgatc ctacagccca gttcggatcg ctcgccccgg
     2161 gtctgtaggg caagaccttg tgtcttgccg cgaatgccga tcacttgaac cgggtgcgac
     2221 gcccgggatc gccaccgacc tgaaccgcgc tcttgagggc acccgttgcc gacccggtgt
     2281 ggggaaggcg gcctccggcc gcgggcgaac acgaggttcg cccctacgag gtgttgggaa
     2341 ggcgatgtgc gacggcgggc gagcaccaga gcgcggccga atgggttcgc ccctacggga
     2401 tgacgccggg tcagggttgg ccagggattg cgcggaggat gaggcgtgaa acggttcctc
     2461 gggctctact acggctggcg gattaccgcg gcgctcgccg tcaccgagac gatctcctgg
     2521 ggcatcctct actacgcctt ctccgtcttc ctcgtgccga tgcacgacga tctcggctgg
     2581 tcggtgacgg caatgacggg cgcctactcg ctggcgctcc tcgtctccgg actcctcgct
     2641 ccactcgttg gccgctggct cgatcgacgc gggccgcggg gaatcatgac cgctggctcg
     2701 gccgtcggcg tcatcgcgct ccttacctgg tcccgcgtcg attcgatcgc cggattttat
     2761 gtcctgtggg tggggatcgg catcgcgatg tcgacgacgc tctacgagcc cgccttcgcg
     2821 acgctgacgc gctggttctc ccgcggacgg agccgggcgc tgctgatcgt gacgatcgcc
     2881 gccggcttcg cgagcacgat cttccttccc ttcgccagtc tcctggtcga gcatctgggg
     2941 tggcgccatg ccctgctcgt gctcgccgct atcctcggca tcacggcgat tccactccac
     3001 gggttcatcc tgcgccgtcg ccccgaggat ctcggtctga ccgtcgatgg cgatcccggc
     3061 tccgagacg
//
LOCUS       DASTMD010000474         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_936575, whole genome shotgun sequence.
ACCESSION   DASTMD010000474 DASTMD010000000
VERSION     DASTMD010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936575"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VFQ54_06900"
     CDS             complement(<1..1230)
                     /locus_tag="VFQ54_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0164757.1"
                     /translation="MSAESTLVSITSDYETQFATSKAWNDRAKAVIPSGITHDGRHLS
                     PFPPYVTHAEGAYKYDADGHKLIDYAVGHGSLIFGHSDPDILAAMSGQVGKGTHFSAG
                     HLAEIEWAERIVSLIPSAEQVRFTASGTESTLLAMRVARGYTGKNTILKFEGHFHGWQ
                     DYALKGEKPPFEKTTVPGIPEETLGTVAVVPSNELAMLEERLTQGDVAAVIMEPSGGS
                     WATIPLKEDFLRNVRSLTEKYEAVLIFDEVITGFRWAPGGAQERFGVIPDLTTMAKIV
                     AGGMPGGAVAGKTDLMQMIAFKSEPGWNENRKVLQAGTYNGNPLAAAAGVACLTKVAD
                     KRVHDHCDRLAANIRIGLNEIFTKKGVPAFAWGESSVFHISFGNRAANQTAGDLHTPE
                     GISAEDLKKSGGSKLAQV"
     gene            1561..2847
                     /gene="hemA"
                     /locus_tag="VFQ54_06905"
     CDS             1561..2847
                     /gene="hemA"
                     /locus_tag="VFQ54_06905"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEU0164758.1"
                     /translation="MASEASAAAGATPALVVVGSNHEYAPVSVRERLAFAGEALTHGL
                     QALHERVGEGLILSTCNRTEIYVISDNEEQAREGIFGFLSGYHHVPLPVLERASYVFS
                     GNEAVDHMFRVASGLDSMVLGEPQILSQIRDALDAARDASSVGPLLQRLATDALRVGK
                     RARTETDISRNRNSIAHAAVDLAERELGGLEGRSAVILGAGNMATLAAKLLRARGIGR
                     MTIVNRSFNRATELADATDADAAPMSDLAATIAGADVVFGAATAEAPLITLETILPRT
                     SPLIAIDLSVPRVIAPECELLDGVVVRDVDALEPAIEETRRQYASEVTKVEGLVESAV
                     SSFATWSQSRATTTAIAAIRHHAEQIRDQEVERTLRKLAHLNDRDRNLVRALAVGITN
                     KLLHQPVQSLKAAESLQDVQLALDLFGLGMDPIPEA"
BASE COUNT          498 a          916 c          987 g          553 t
ORIGIN      
        1 gacctgggcg agcttgctgc caccactctt cttgagatcc tcggcggaga taccctctgg
       61 cgtgtgaagg tcgcccgcgg tctgattcgc ggcgcgattg ccgaaggaga tgtggaagac
      121 cgagctctcg ccccaggcga aggccggaac gcctttcttc gtgaagatct cgttcaggcc
      181 gatccggata ttcgcggcga ggcgatcgca gtgatcgtgc acgcgcttgt ccgcgacctt
      241 ggtcaggcag gcgacgcccg ccgccgcggc gagcgggttg ccgttgtacg taccggcctg
      301 gaggaccttg cggttctcgt tccagcccgg ctcgctcttg aaggcgatca tctgcatgag
      361 gtcggtcttg ccggcgacgg cgccgcccgg catgccgccg gcgacgatct tcgccatcgt
      421 cgtcaggtcg gggatcacgc cgaaccgctc ctgggcgccg cccggggccc agcggaagcc
      481 ggtgatgacc tcgtcgaaga tgaggaccgc ctcgtacttc tcggtcaggg agcggacgtt
      541 ccgcaggaag tcttccttca gcggaatcgt cgcccaggag ccgccgctcg gctccatgat
      601 caccgcggcg acgtcgccct gggtcaggcg ctcctccagc atcgcgagct cattcgacgg
      661 caccacggcg accgtgccga gcgtctcttc cggaatgccg gggacggtcg tcttctcgaa
      721 cggcggtttc tcgcccttca gcgcgtagtc ctgccagccg tggaaatgcc cctcgaactt
      781 gaggatcgtg ttcttgccgg tgtagccacg agcaacgcgc attgcgagga gcgtcgactc
      841 ggtgccggat gccgtgaagc gaacctgctc ggcggacggg atcagcgaga cgatccgctc
      901 cgcccactcg atctcggcga gatgcccggc gctgaagtgg gttcccttgc cgacctgacc
      961 gctcatcgcg gcgaggatgt ccggatcgct gtgaccgaag atgagcgagc catgccccac
     1021 ggcgtagtcg atgagcttgt ggccatcggc gtcgtacttg taggcgccct cggcgtgcgt
     1081 gacatatggg gggaacgggc tcagatggcg tccgtcatgc gtgatgccgg atgggatgac
     1141 ggctttggcg cggtcgttcc aggccttgga cgtggcgaac tgggtttcat agtcggacgt
     1201 aatcgagacc agggtggatt ccgcggacat ggatatccct cgtgagtgat tgcccgaacg
     1261 ggcctcgcga gcacgccaga ttagcgccaa ggtgatgcaa gaggcccatt gcgagacgcg
     1321 ccggaagcct cggcctgtcc gtgcactata gagccgacgc tggatccgcg caaaggtgtt
     1381 tttcggtggc gttgcaaccc acccacccgt tgaattcact tgagaattat tcttgcagtg
     1441 gtgcacgggg agacggttgt gggcgtgtat ctcgggtatc cttgtcggga cctgagtacg
     1501 ccggatcgct agggtcgata tggcccggct ggcgctggtt tgtggggcga ttctagaacc
     1561 atggcatcgg aagcatcggc ggcggcggga gctacaccag cgctcgttgt cgttggatcg
     1621 aatcatgagt atgcgccggt gagcgtgcgc gaacgtctgg cgttcgcggg cgaagcgttg
     1681 acgcatgggc tgcaggcgct ccacgagcgc gtgggtgagg ggttgatcct ttccacctgc
     1741 aatcgcaccg agatctacgt catcagtgat aacgaggaac aggcgcggga ggggatcttc
     1801 ggatttctct ccggctacca tcatgtgccg ctgcccgtcc tggaacgggc gtcctacgtc
     1861 ttctcgggaa acgaagccgt cgatcacatg tttcgagtcg caagcggtct cgattcgatg
     1921 gtgctcggcg aaccgcagat cctgagccag atcagggatg ccctcgacgc cgcgcgcgac
     1981 gcctcgtccg tcggaccgct gctgcagcgc ctcgccaccg acgccttgcg ggtgggcaag
     2041 cgcgcccgca ccgagaccga catctcgcgg aatcgcaact cgatcgccca cgccgcggtc
     2101 gatctcgcgg agcgggaact cggtggactc gaagggcggt ccgcggtcat cctcggggcc
     2161 ggcaacatgg cgaccctcgc ggcgaagctc ctgcgggcac gcggtatcgg gcgaatgacg
     2221 atcgtgaacc gatccttcaa tcgcgctacc gaactcgcgg atgcgactga cgccgatgcc
     2281 gcgccgatga gcgacctggc ggcgacgata gccggagcgg atgtcgtttt cggagcggcg
     2341 accgctgagg cgccgctgat cacgctcgag acgattcttc cccgaacttc cccgctgatc
     2401 gcgatcgacc tcagcgttcc ccgcgtgatc gcccccgaat gcgaacttct cgacggcgtc
     2461 gtggtgcgcg acgtcgatgc gctggagcca gcgatcgagg agacgcgacg tcagtatgcg
     2521 tccgaagtca cgaaggtcga aggtctcgtc gagtctgccg tctcgtcgtt cgcgacgtgg
     2581 agccagagtc gggcgacgac gaccgcgatc gccgcgatcc gtcatcacgc cgaacagatt
     2641 cgcgatcagg aagtggagcg tacgttgcga aagctcgcgc acctcaacga tcgcgatcga
     2701 aacctcgtgc gggcgctggc ggtggggatc accaacaagc tgctccatca gccggtgcag
     2761 tcgctcaagg cggccgaatc cctccaggac gtccagcttg cgctggatct gttcggactc
     2821 gggatggacc cgatccccga agcgtgatct gggtcgctgg attacagaat cgaaccaacg
     2881 ctccgcatat ccgtcgttgc agggcaatga cgacctgttc atgacgtttc catcgatgga
     2941 ggaagttgtg ccgt
//
LOCUS       DASTMD010000475         1601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1562153, whole genome shotgun sequence.
ACCESSION   DASTMD010000475 DASTMD010000000
VERSION     DASTMD010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562153"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFQ54_06910"
     CDS             complement(<1..433)
                     /locus_tag="VFQ54_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164759.1"
                     /translation="MLGASLAIGLLYFLPRYDDIHRVVTVDEPLWLGRSANFLDGLLF
                     LHPLDTYQFAHPGVMTMWAGALAYLVMQPAYMLHDFKYTTHTTDIHKQLRAFDIDPLQ
                     MLVYARVSKLLLQTGLFIFAIWMIDRLFGRRVAVGTAITIAF"
     gene            complement(661..>1601)
                     /locus_tag="VFQ54_06915"
     CDS             complement(661..>1601)
                     /locus_tag="VFQ54_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0164760.1"
                     /translation="SYKGDAAQLRELALFEDVFALITDRQGIGGERVALKVVQGLVGG
                     PGLDAAFRIANPLLPGNRKKPTYRVIARMSGDHIYRRTDLRTTVERGVQARMPDWGLV
                     EDARVEIWAQLVGDRLVAGLRLSDETMRQRTYKMVSLPASLKPTIARAIVTLSRPEDD
                     DVFLDPMCGAGTILIERAHAGRYGRLEGGDIDHVAVNATRENFGTRHQPFTIREWDAR
                     ELPFADGEISAIGSNLPFGKQIGSRADNQLLYPAVLKEWARVLKPGGRMVLLTSESRL
                     LRATLRDRAEFIWEQTVPIVVRGQPAEIFVLMRATD"
BASE COUNT          287 a          493 c          556 g          265 t
ORIGIN      
        1 cgaaggcgat cgtgatcgcc gtgccgaccg caacccggcg tccaaagaga cgatcgatca
       61 tccagatcgc gaagatgaag agcccggtct ggagcagcag tttcgagacg cgcgcgtaga
      121 cgagcatctg cagcggatcg atgtcgaagg cccggagttg cttgtggatg tcggtggtgt
      181 gcgtcgtgta cttgaagtcg tgcagcatgt aggcaggctg catgaccagg taggcgagcg
      241 cgcccgccca catcgtcatc acgccgggat gggcgaactg gtaggtatcg agcgggtgga
      301 gaaagagcaa cccgtcgaga aagttcgccg agcgtccaag ccagagcggt tcgtcgaccg
      361 tcacgacgcg atggatatcg tcatagcgtg gaaggaagta gagcaagccg atcgcgagcg
      421 aggcaccgag cacgctccag attccgaagg agcgatcgcg gacccatcgg gaacgggcat
      481 cgtcaggcgt tggtattccg gggatgtccg aagggagcga cggatcctgc gcgccggcgc
      541 caggcgtgag ttggtctatg gcgcgtttct cctcgcgcga cggcgcacac cccacgtcgc
      601 gaccacaaca ccggtgatgg tctacaggga cccagattgc atgatatcgc gctggctgcc
      661 ctaatcggtg gcgcgcatca gcacgaagat ctccgcgggc tggccgcgga cgacgatcgg
      721 caccgtctgc tcccagatga actccgcgcg gtcgcgcagc gtcgcccgca ggagccgcga
      781 ttcgctggtc agcagcacca tgcgtccgcc tggtttcagg acgcgcgccc actccttgag
      841 tacggcggga tagagcaact ggttgtctgc gcgggagccg atctgcttgc cgaacgggag
      901 gttggacccg atcgccgaga tctctccgtc ggcgaagggc aactcgcggg catcccattc
      961 ccggatcgtg aagggctggt ggcgcgttcc gaagttctcc ctggtcgcgt tgacggcgac
     1021 gtggtcgata tcgcctccct cgagccggcc gtaacggcca gcatgcgcgc gctcgatcag
     1081 gatcgtcccc gcgccgcaca tgggatcgag gaagacatcg tcgtcctcgg gtcgggagag
     1141 ggtgacgatc gcacgggcaa tggtgggctt caatgaggcg gggaggctga ccatcttgta
     1201 ggtgcgctgg cgcatcgtct cgtcggagag acgcaggccg gcgaccagcc ggtcgcccac
     1261 gagttgcgcc cagatctcga cgcgggcgtc ctcgacgagg ccccagtcag gcatcctcgc
     1321 ctgcacgccg cgttcgaccg tcgttcgcag atcggtgcgg cggtagatgt ggtcgccgga
     1381 catccgggcg atgacgcgat acgtcggctt cttgcgattt cccgggagga gtggattggc
     1441 gatccggaag gcggcgtcca gtcccgggcc gccgaccagt ccctggacga ccttgagcgc
     1501 cacccgctct ccaccgatgc cctggcggtc ggtgatcagc gcgaagacat cctcgaagag
     1561 cgcgagctca cggagctgcg cggcgtcccc cttgtacgag a
//
LOCUS       DASTMD010000476         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1918985, whole genome shotgun sequence.
ACCESSION   DASTMD010000476 DASTMD010000000
VERSION     DASTMD010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918985"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..2768)
                     /locus_tag="VFQ54_06920"
     CDS             complement(228..2768)
                     /locus_tag="VFQ54_06920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0164761.1"
                     /translation="MPDIGDTSDGVAPTRAPERSSERQAGVKVRAAATALVLSILIAL
                     LYLVPRLVDINRMVVVDEALWLGRSANFAYGLVHFHPMETYQFAHPGVTTMWAGAIAM
                     FVMENVFVRHHDMPINSVVNVHEALRNYGIDPLDMLVYARVTKLLGQALLFVIAFWLI
                     VRIFGRWVAIVAAVMIACDPFLIGHDRLLHIDGLLSITSFTTLLAFIYARQRRANVWP
                     WVLCGVLFTLTCLSRSVGGILLPIFAAIVILPPAIAAIRRRLDIEAAWSQIKTPVLGF
                     IVPAVISAWILWPALIWAPGIVVTDMLSFSITAVDEGHELPTYFNGKSVGGDPGWIFY
                     PETMLWRMTPFTLAGVAILVVALLLRRERIVPRSYRGPIAILGAFAAIFTFGMTLADK
                     KFDRYVLPVYPIAELFAAIAVVGVARLCWERRSTIPNIVAAIAILLAVGGQAQAAYSD
                     RTYGLDYYNPLLGGMKGADDSMLIGWGEGLDQAADYIEAQPDGAYAIVRTSANRVSLL
                     YFVPETTDVEAGGLTASATGIANWAETDYYVSYTLQWQRNLTQMVLHQLAGYQPVHVV
                     SIDGVEMARVYKLEDIPPPQKMVDSLNCTWDFGEQVRLLTFTDIPNPVPQAAQSTTNQ
                     IINIIKSITGANASETPTPTATTPVTTPAPTATPPPTPTTPASPSPVAPASASATPGT
                     PAPATPAPSPTPTAKIVVTPTPAPTSESNGAAAGPPLLPGRVVAPTSGPNESRLVTLI
                     FATGASYAGTGSFDVKVTLVPKAGIQSPIVETTTLTPSTVPGALREATVNLTIPANRD
                     LNSYWMEVTVSDPATGKVLPGELLDAKGNPVPKSSTIVNLCSPTQPKG"
     gene            complement(2936..>3212)
                     /locus_tag="VFQ54_06925"
     CDS             complement(2936..>3212)
                     /locus_tag="VFQ54_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164762.1"
                     /translation="SNPLDVTVTFVPKGSLLPPIVATSTLIPSNVPGALRSVPLDVSF
                     PGSETAASYDMTVTVTDPVTGEQLASSSVEKDGTTTPGPAAMNACES"
BASE COUNT          568 a          982 c         1117 g          545 t
ORIGIN      
        1 gtcgacacgc ttggtgctgc aggggttggt agggcggggt tttatacccc gccgcaaagc
       61 cgacttcggc agaaacacgt cccgcgccaa ggagcgccga ccgagacccg ctctccaccc
      121 cgccgcgacg cggattcggc agagacacgc cccgccgcaa ggccgacttc gggagagtca
      181 cgcctcgccc cgagacggca tcgagccgga tctggctcca ggagacgtta tcccttgggc
      241 tgcgtcggcg aacagagatt gacgatggtc gagctcttgg ggaccggatt gcccttcgcg
      301 tcgaggagct cgcccggcag gaccttgccc gtggcgggat cggaaaccgt cacttccatc
      361 cagtagctgt tcaagtcgcg gttcgccggg atcgtcagat tgacagtcgc ttcccgtaag
      421 gcccccggaa cggtcgacgg tgtgagcgtc gtggtctcga cgatcgggct ctggatgccc
      481 gccttcggca cgagggtcac cttgacatcg aaggagcccg ttcccgcgta cgaggcgccg
      541 gtcgcgaaga tcagggtgac gaggcgactc tcgttcggcc cggaggtcgg cgcgacgacc
      601 cgaccgggaa gcagcggcgg accggcggcg gcaccgttcg actccgatgt cggggcagga
      661 gtcggggtga cgacgatctt cgccgtcggc gttggcgagg gtgccggggt tgccggagct
      721 ggtgtgcccg gtgtcgccga cgctgaggct ggagcgaccg gcgacggaga tgccggggtc
      781 gtcggtgttg gcggcggcgt cgctgttggc gccggcgtgg ttaccggcgt ggttgcggtc
      841 ggtgtcggcg tctcgctggc gttggccccg gtaatcgact tgatgatgtt gatgatctgg
      901 ttggtcgtgg actgggcggc ctgcggaacc ggattcggga tgtccgtgaa ggtgagaagc
      961 cggacctgct cgccgaagtc ccacgtgcag ttgagactgt cgaccatctt ctgcggcggt
     1021 gggatgtcct ccagcttgta gacgcgagcc atctccacac cgtcgatcga gacgacgtgg
     1081 acgggctgat atccggcgag ctgatgcagc accatctgcg tcagattgcg ttgccactgc
     1141 agtgtgtagg agacgtagta gtccgtctcc gcccaattgg cgatgccggt ggcgctcgcc
     1201 gtaagcccgc cagcctcgac atcggtcgtt tccggtacga agtagagcag cgagacgcga
     1261 ttggccgacg ttcggacgat cgcatacgcg ccatcgggtt gcgcctcgat gtaatccgcc
     1321 gcctggtcaa gtccctcacc ccagccgatc agcatcgaat cgtccgcgcc cttcatcccc
     1381 ccgagcagcg gattgtagta gtcgagtccg taggtgcgat cgctgtaggc cgcttgcgcc
     1441 tggccaccga cggcgagcag gatcgcgatt gcggcgacga tgttggggat ggtggaccga
     1501 cgctcccagc agagtcgcgc gacgccgacg acggcgattg ccgcgaagag ctcggcgatc
     1561 ggatagacgg gcaggacgta gcggtcgaac ttcttgtcgg caagcgtcat tccgaacgtg
     1621 aagatcgcgg cgaaggcgcc cagaatggcg atcggcccgc gatacgatcg agggacgatg
     1681 cgctcgcggc gaaggagcag cgccactacg agaatcgcga ccccggccag ggtgaacggc
     1741 gtcatccgcc agagcatggt ctcggggtag aagatccagc ccggatcgcc gccgacgctc
     1801 ttcccgttga agtacgtggg tagctcgtgg ccctcgtcga ccgcggtgat cgagaagctg
     1861 agcatgtccg tcacgacaat ccccggcgcc cagatcagcg ccggccagag gatccaggcg
     1921 gagatgactg ccggcacgat gaagccgagc accggcgtct tgatctggct ccaggcggct
     1981 tcgatgtcga gacgccggcg gatggcggcg attgccggcg gcaggatcac gatcgcggcg
     2041 aagatcggga gcaggatccc tccgaccgaa cgcgacaggc aggtcagcgt gaagagcacg
     2101 ccacagagca cccacggcca gacgttggcc ctcctctgcc gggcgtagat gaaggcgagc
     2161 agcgtcgtga acgacgtgat gctcagcagg ccgtcgatgt gaagcaggcg atcgtggccg
     2221 atcaggaagg ggtcgcaggc gatcatcacg gctgccacga tcgccaccca gcgcccgaag
     2281 atgcggacga tgagccagaa ggcgatgacg aagaggagag cttgtccgag gagcttcgtg
     2341 acgcgagcgt agacgagcat gtcgagcggg tcgataccgt agttgcgcaa tgcctcatgg
     2401 acattgacga cgctgttgat cggcatgtcg tggtgacgaa cgaagacatt ctccatcacg
     2461 aacatcgcga tcgccccggc ccacatcgtg gtgacgccgg gatgcgcgaa ctggtacgtc
     2521 tccatcgggt ggaagtggac gagaccgtag gcgaagttcg ccgagcgacc gagccagaga
     2581 gcctcgtcca cgacgaccat ccgattgata tcgaccagac ggggaaccag gtagagcagc
     2641 gcgatcagga tcgagaggac aagcgcggtc gcggcggcac gtaccttcac gccggcctgg
     2701 cgttccgacg atcgttccgg agcgcgggtt ggggcgacgc cgtcgctcgt gtcgccgatg
     2761 tcaggcatca tgttcgccca atctctcgcc ccatcctcgt cgcattcgcc cctgcaggtt
     2821 gacgcactcc cggtgctttt gtgggctccg gctcctcgac gcagtatgtc atgacccgca
     2881 ccgccggcga gacttcatgc ggacccgctc gcttcttgaa cgtgaagaaa cggcatcagg
     2941 attcgcaggc gttcatcgcc gccgggcccg gggtcgtggt cccatccttc tctacgctgg
     3001 aactcgcgag ttgttccccc gtgaccgggt cggtcacggt cacggtcatg tcatagctcg
     3061 ccgcggtctc cgatcccgga aaggagacgt cgagcgggac cgatcgcagg gcgccgggca
     3121 cgttcgacgg gatcaacgtc gatgtcgcca cgatcggggg caggagcgat cccttcggga
     3181 cgaatgtgac tgtgacgtcg aggggattgc tg
//
LOCUS       DASTMD010000477         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1384526, whole genome shotgun sequence.
ACCESSION   DASTMD010000477 DASTMD010000000
VERSION     DASTMD010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384526"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1409)
                     /locus_tag="VFQ54_06930"
     CDS             complement(3..1409)
                     /locus_tag="VFQ54_06930"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEU0164763.1"
                     /translation="MSQGETRTVRDSMGEMQVPANVLYGAGTARAVENFPISGLRFSR
                     AFLHALGLIKASTATVNRDLGLLDPAIADLVERAALEVADGAWDAEFPIDIFQTGSGT
                     STNTNANEVISTRATQLDANVKIHPNDHVNMGQSSNDVIPGAIHVSAYIAVAEQLLPA
                     LEHLAATFEKRADETRGIIKTGRTHLMDATPVRLGDEIGGWAAQVRNAAARITSTLPR
                     VAKLAIGGTAVGTGLNADPGFGRLVAAELSRRTGHPFVETDNHFEAQASQDTAVELSG
                     QLKVVAVALIKIANDLRWMNSGPQSGLGEIVLPALQPGSSIMPGKINPVIPEATMMVA
                     AQVIGNDATITTGAQLGNFELNVMLPVIAHNLLQSIELLASVSTVLADKAVAGFTVNT
                     YHIGDLVEKNPIMVTGLNPIIGYDNAAKIAKRAYAENRRVKDVALEETELTEEELAHL
                     LDPAELADPGIKGAASGG"
     gene            complement(1605..2000)
                     /locus_tag="VFQ54_06935"
     CDS             complement(1605..2000)
                     /locus_tag="VFQ54_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU0164764.1"
                     /translation="MPKGPVPAQFHDILESTGLAHVATIGRDGRPQNNPVWFLWDGEH
                     VLLSFIEAKQKYQNLLKNPAVAISIADPNNPYHYVELRGRLVSTEVDEDRAFIDTIAK
                     KYTGQPFGLHKEGEVRLRSVIEIDSWTGQ"
     gene            complement(2102..2497)
                     /locus_tag="VFQ54_06940"
     CDS             complement(2102..2497)
                     /locus_tag="VFQ54_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU0164765.1"
                     /translation="MPKGPVPAKFHPLLESAALAHLATIGANGHPQVNPIWFLWRGES
                     IQFGVKDGTAKLENIRRDPHVALSILDRDDPGHYVELRGEVTGIELYLDLSLVNELAR
                     KYTGEDFTGGKVGEPRYRLTMRVDSWTGQ"
     gene            complement(2500..2901)
                     /locus_tag="VFQ54_06945"
     CDS             complement(2500..2901)
                     /locus_tag="VFQ54_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU0164766.1"
                     /translation="MPVEIPADFHPLLESRAIAYVSTLGAKGEPQTSPIWFMWDGETV
                     SFSLVDGRQKLKNLRRDPRISVAIADLEVPTWYIELRGRIDDLVEDPGRELERAIAIK
                     YTGEDFDVDPAGTMRYDARVIVEKITFQRGI"
     gene            3082..>3286
                     /locus_tag="VFQ54_06950"
     CDS             3082..>3286
                     /locus_tag="VFQ54_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0164767.1"
                     /translation="MAIEVSIMIEGQDGLSWPRWQRIARAAEELGFAGLYRSDHFTNP
                     QGPVLDDIETWVSLAWLASHTSRI"
BASE COUNT          571 a         1044 c         1020 g          651 t
ORIGIN      
        1 gatcaaccgc cgctcgccgc gcccttgatt cccgggtccg cgagttccgc cggatcgaga
       61 agatgcgcaa gctcctcctc tgtgagctcc gtttcctcga gcgcgacgtc cttcacacgc
      121 cggttctcgg cgtaggcacg cttcgcgatc ttcgccgcgt tgtcgtagcc gatgatcgga
      181 ttcaggccgg tgaccatgat cggattcttc tcgacgagat cgccgatgtg atacgtgttg
      241 acggtgaagc cggcaacggc cttgtccgcg agcacggtgc tcacactggc aagcaactcg
      301 atcgactgca gcaggttgtg ggcgatcacc gggagcatca cgttcagttc gaaattcccg
      361 agctgggcgc cagtcgtgat cgtcgcgtcg ttgccgatca cctgggcggc aaccatcatc
      421 gtcgcttccg ggatcacggg attgatcttg cccggcatga tcgacgatcc cggttgcagc
      481 gccgggagca cgatctcgcc cagtcctgat tgcggaccac tgttcatcca gcgcagatcg
      541 ttggcgatct tgatcagcgc gacggcgacc accttgagct gcccgctaag ttcgaccgcg
      601 gtgtcctgcg acgcctgcgc ctcgaaatga ttgtccgtct cgacgaacgg atgcccggtg
      661 cggcgcgaga gttccgctgc cacgagacgg ccgaaacccg gatcggcgtt gagaccggtg
      721 ccgacggccg taccaccgat tgccagcttc gcgacccggg gaagcgtgct cgtgatccgc
      781 gccgcggcgt tccggacctg ggcggcccaa ccgccgatct cgtcgccgag tcgaaccggc
      841 gtcgcgtcca tcaggtgggt acgtccggtc ttgatgattc cccgcgtctc atcggcgcgc
      901 ttctcgaacg tcgccgcgag atgttcgagc gccggcagca gctgctccgc gaccgcgatg
      961 tacgccgaga cgtgaatcgc gcccggaatc acatcgttgc tgctctgtcc catgttgaca
     1021 tggtcattgg ggtggatctt cacgttcgcg tcgagctggg tggcccgggt cgagatcacc
     1081 tcgttcgcgt tcgtgttcgt ggatgtcccg gaaccggtct ggaagatgtc gatcgggaat
     1141 tccgcatccc aggcgccgtc ggctacctcg agcgcggcgc gctcgacgag atcggcgatg
     1201 gcgggatcga ggagaccgag atcgcggttg accgtcgccg tcgaggcctt gatcagacca
     1261 agggcatgga ggaacgcccg cgagaagcgg agaccgctga tcggaaagtt ctcgactgcg
     1321 cgtgccgtcc ccgcgccata gagcacgttc gccggcacct gcatctcacc catgctgtca
     1381 cggacggttc tggtctcacc ctgactcatg cacatcccct tcgtatcgca tgtcatcgcc
     1441 gccgccccgc cgggcggttc ggcgtgcgca tcctcgcgca tcatcggagg tccagtgtac
     1501 cgccgccgcg atagctgcgg tgaggcttcg gcgaacgcaa cggcgcccgg acctgtggca
     1561 tcaggtccgg gcgccgtctt cgagtcagat cgagttgcct gccgctattg ccccgtccag
     1621 gagtcgattt cgatcacact gcggaggcga acttccccct ccttgtgcag accgaacggc
     1681 tggccggtgt atttcttcgc gatggtgtcg atgaaggcgc gatcctcgtc gacttcggtc
     1741 gagacgagac ggccacgaag ctcgacgtag tgatacgggt tgttcggatc ggcgatcgaa
     1801 atcgcgaccg ccggattctt caggagattc tgatacttct gcttcgcctc gatgaagctc
     1861 agcagaacgt gctcgccgtc ccagagaaac cagaccgggt tgttttgcgg cctgccatcc
     1921 cgaccgatcg tggcgacatg tgccaggccg gtactctcga gaatgtcatg aaattgcgcg
     1981 ggaacggggc ctttgggcat gtcgccttca ccttctggtt ggcacgaatg gatacgaccg
     2041 tcctcctgga cgaccgcaat gatagtactt acgaattgtt agctcgtcaa ggtcggcgcg
     2101 ttcattgacc ggtccaggaa tccacccgca tcgtcaaccg gtagcggggc tccccgacct
     2161 tgccaccggt gaagtcctcg ccggtgtact tccgggcgag ttcattcacc agcgagaggt
     2221 cgagatagag ttcgatgccc gtcacctcgc cgcgaagctc gacgtagtga ccaggatcat
     2281 cgcgatcgag aatcgagagc gcaacgtgtg gatcacgccg gatgttctcc agcttcgcgg
     2341 tgccatcctt gaccccgaac tggatgctct cgccccgcca gagaaaccag atcgggttca
     2401 cctggggatg cccgttcgcg ccgatcgtcg cgaggtgggc gagcgcggca ctttcgagca
     2461 gcggatggaa tttcgcgggt accgggccct tcggcatggt catatccctc gttgaaaggt
     2521 gatcttctcg acaatgacgc gtgcgtcgta gcgcatcgtc cccgcaggat cgacatcgaa
     2581 atcctcaccg gtgtacttga tggcgatcgc ccgctccagc tcgcgaccgg gatcctcgac
     2641 gagatcgtcg atgcgaccgc gcaactcgat gtaccaggtg ggcacctcga gatcggcgat
     2701 cgcgacggag attcgcgggt cgcggcgcag gttcttcagc ttctgccggc catcgacgag
     2761 actgaaactc accgtctccc catcccacat gaaccagatc ggtgacgtct gcggctcgcc
     2821 cttcgcgccc agcgtcgaga cgtaggcgat ggcccttgat tccagcaggg gatggaaatc
     2881 cgcaggtatt tcgacaggca tgggatcggt ggctccttcc gtcaaatcgc gggcgcatct
     2941 tcgctccact gtacagtgtg ctacacgacc attaagatca atgcggatct cgaccgaccg
     3001 gctgtctgcc tcaggcaacg gacgaacgct atggaggcgc cctgatgcgg ggcgtcacag
     3061 caagaaaagg atgacagcca gatggcgatc gaagtttcga tcatgatcga gggacaggac
     3121 ggcctttcgt ggccgcggtg gcagcgaatc gcgcgcgcgg cggaggaact cggtttcgcc
     3181 ggtctctatc gttcggacca cttcacgaac ccgcagggac ccgtgctcga cgacatcgag
     3241 acctgggtct cgctggcctg gctcgcctcg cacacgtcgc gtattt
//
LOCUS       DASTMD010000478         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_580644, whole genome shotgun sequence.
ACCESSION   DASTMD010000478 DASTMD010000000
VERSION     DASTMD010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580644"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..467
                     /locus_tag="VFQ54_06955"
     CDS             228..467
                     /locus_tag="VFQ54_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU0164768.1"
                     /translation="MATVLERIQGIVAERLGVEESQVVESAEFIGDLNADSLDLVEVI
                     MAMEQEFDLEIKDEDAENIRTVGDAVKYIEEHQES"
     gene            1045..1944
                     /gene="tatC"
                     /locus_tag="VFQ54_06960"
     CDS             1045..1944
                     /gene="tatC"
                     /locus_tag="VFQ54_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU0164769.1"
                     /translation="MSTLPKRIGVGSARAIARTARAARDPLKPVRAIRDIYRPEDPDT
                     PEVFEEMTLQEHLVELRDRIMKIVIALVLAMIVGFYFAGGILENIKDKANAEQGLDVK
                     SPTDPLTLTFKVAFYVAIAIIMPIIIYQLVGFLSPGLTRREKRVLFSALPFVSLLFIG
                     GAAYGYFVAAPRALYFLSTWNLGAFTWQPDGPEVITFFLTLMIGLGLAFQLPVIMFIL
                     AKIGIVTARKMRQWRRYAYLVLCIAAAVITPSTDPFNMAIVAVPLIVLYEFGLIITTL
                     FAKTSLRDKLPDAADAASPTGGA"
     gene            complement(2244..>2844)
                     /locus_tag="VFQ54_06965"
     CDS             complement(2244..>2844)
                     /locus_tag="VFQ54_06965"
                     /inference="COORDINATES: protein motif:HMM:NF017482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HEU0164770.1"
                     /translation="RKAQKAGDQLPERIEESEREREYLLQLRLQAEQSDGFAAIEAIR
                     QEFEEHNGGRNPVGEKNPQRGKKQQTRRVTPMIDDDGNLIYIGRSGRENDQVTFDIGG
                     PNDTWLHARGVPGSHVIVRWVRPDNESEESIETAAALAAYYSGSRGSGTVEVDIAKRR
                     DVRKIKGAGPGMVTYRNEYTIPVRPLSEVDLKQAGRLEK"
BASE COUNT          494 a          946 c          837 g          567 t
ORIGIN      
        1 gtcgtccagc gagggatgat tttcctaccc ctgaggcggg cggttcgtgt atcatccacc
       61 cgatatcatg cgttctagag cgtcgtttga tagattcttc gggaaccggt ttggcgaggc
      121 gcacggaacg tacggtgggc ggacatatgt gtccgcaatt cgtttgagtc cggacagagg
      181 ctgaagcaag ccggttcgca tcattgtctc gttgaggagg atttaggttg gcaaccgtac
      241 tggagcgaat tcagggcatc gtcgccgagc gattgggtgt tgaagagagc caggtcgtcg
      301 aatccgccga gttcatcggc gatctcaacg ccgattcgct tgacctcgtc gaggtcatca
      361 tggcgatgga gcaggagttc gatctggaaa tcaaggacga ggatgcggag aacatccgca
      421 ccgttggtga tgccgtcaag tacattgaag agcatcagga gtcctaggcc tcgcgagtgg
      481 atgcggacga cctgtgacag cacggggagt gtgtgacggt atcggcagat tcgccgttcg
      541 accgcgctcc caaccaaacc ggtgcattgc actggcacaa tggcgaaccg tgtatccatt
      601 ctggtcgtct cgacaccgct gacagaatcc gtgaaccatc ctcgcgccaa ggtagctcca
      661 tcacgtcgat tccgggtcga tgcacccagg gggatcggga gtgacaaaca tgagccaggc
      721 gcacgatcaa cgtccaccat caggtgaccg cgacgacctc gcaccccgtg cgatgcgtct
      781 ctccttgcgc tccctcccct gccctcaggt catctcccca cgatcttcag tcgatgtccg
      841 tcgcggcccc gtgccgcgcg gcatgatgca tccggccccg gctcccgttc gtcgccctgc
      901 gatgagtggt gactcgaagc cggatgttcg tttcggcgca gcgggacagg tcgtctcatc
      961 actcgccgga gcctctgatg gcattgcgac gacgcgttcg acggcagcag caggccgatc
     1021 gaacaaggca gggggagtca cagcatgagc accttaccga agagaattgg tgtcgggtcc
     1081 gcccgcgcca tcgcgcgaac agcccgcgcg gcccgcgatc cgctgaaacc ggttcgcgcg
     1141 atccgtgaca tctatcggcc cgaggatccg gacactcccg aagtattcga ggagatgacg
     1201 ctccaggagc atctcgtcga gctccgcgac cgcatcatga agatcgtgat cgcgcttgtc
     1261 ctcgcgatga tcgtcggctt ctactttgcc ggcggcattc tcgaaaacat caaggacaag
     1321 gcaaacgccg agcaggggct tgacgtcaag agcccgaccg atcccctgac cctgaccttc
     1381 aaggtcgcct tctacgtggc gatcgcgatc atcatgccga tcatcatcta ccagcttgtc
     1441 ggcttcctgt cccccggtct gacacgtcgc gagaagcgag tgctcttttc ggcgctccct
     1501 ttcgtctccc tgctcttcat cggtggcgcg gcctacggct atttcgtcgc cgccccgcgc
     1561 gcgttgtact tcctctcgac ctggaatctc ggcgccttca cctggcagcc ggacggcccc
     1621 gaagtcatca ccttcttcct gacgctgatg atcggtctcg gtctcgcctt ccagttacca
     1681 gtcatcatgt tcatcctcgc caagatcggg atcgtgactg ccaggaagat gcggcagtgg
     1741 cgacgatacg cctatctcgt cctttgtatc gccgctgccg tgatcacgcc cagcaccgat
     1801 cccttcaaca tggcgatcgt cgcggtcccg ttgatcgtgc tctacgagtt cgggctcatc
     1861 atcacgacgc tcttcgcgaa gacctcgctc cgcgacaagc tcccggatgc ggccgacgcg
     1921 gcgagcccca ccggcggcgc atagccgtca cactgacggc gtatcggacg ccatgagtga
     1981 agcagccggg atgccgtcat ggcatcccgg ctccgaccaa accctcctcc cgagctcgtc
     2041 gagggaacgc cccgacgctc ctgtgctctc aatgcaacgc cgccccagtc gtcaattcga
     2101 tcccgtcgag aaatctcccg gcccgacacc gacacgaccg ttgctcgcgc ggtgttcgac
     2161 ggaaatggat cggcgatcga aacgggttgc ccgcgggagc gcgatcccgg ggcgaacggg
     2221 aggcgggaca ggccgtccgg gcgctatttc tcgagacgac ccgcctgctt caggtccacc
     2281 tcgctcagag ggcgcaccgg gatcgtgtac tcgttgcgat aggtcaccat tcccggaccc
     2341 gcgcccttga tcttccggac gtcacgccgc ttcgcgatgt cgacctcgac ggtgccgctt
     2401 ccccgcgatc cgctgtagta ggcggcaagc gccgccgccg tctcgatgct ctcctcggac
     2461 tcgttgtccg ggcgcaccca ccgcacgatc acgtgggacc ccggcacacc gcgcgcgtgg
     2521 agccacgtgt cgttcgggcc accgatgtcg aacgtcacct ggtcgttctc gcgaccggag
     2581 cgaccgatgt agatcaggtt gccatcgtcg tcgatcatcg gcgtcacgcg gcgggtctgc
     2641 tgcttcttgc ctcgctgggg attcttctca ccgaccgggt tccgcccgcc attgtgctcc
     2701 tcgaactcct ggcgaatggc ctcgatcgcg gcaaagccat cggattgctc ggcctggaga
     2761 cgcagctgaa gcagatactc ccgctcgcgc tcgctctcct cgatccgttc cggaagttga
     2821 tcgcccgcct tctgcgcctt ccgg
//
LOCUS       DASTMD010000479         1941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_401598, whole genome shotgun sequence.
ACCESSION   DASTMD010000479 DASTMD010000000
VERSION     DASTMD010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401598"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VFQ54_06970"
     CDS             complement(<1..662)
                     /locus_tag="VFQ54_06970"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEU0164771.1"
                     /translation="MIERFKRYLPFIRKYGTYLWLGAILILAVYVGLNQRDDLRKTWD
                     LIVGADPRWIVSILILEAVSFVLIAAVYGLVLHQLGYRLSLALMISLHLQRAVINGLT
                     PLGGPSSVVVFVTRLRHKGVPPADSLLAAFIKSVSGHVAFLLILLPALFIGDPTFLMF
                     ASTGCLVVLVAAMLTFLSLIMRRRRLPGFLIARIPRRGLRMLVQLRRHRIEPQTLAWP
                     MV"
     gene            complement(763..981)
                     /locus_tag="VFQ54_06975"
     CDS             complement(763..981)
                     /locus_tag="VFQ54_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164772.1"
                     /translation="MAWMVGETMDGQAVLWFAIGVGAACFGLSLLYQRYVKRTPTAVQ
                     SGGSFTMFAILMFLFALGAIAAGVIASA"
     gene            1074..1556
                     /locus_tag="VFQ54_06980"
     CDS             1074..1556
                     /locus_tag="VFQ54_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU0164773.1"
                     /translation="MSDSHRLPPGSVPRYEPPRPVRYTISDLERETGVTGRTIRYYIA
                     EGLLQPAFGRGPSATYDSDHLLRLRLIQQLKDSRLPLAEIKERLAQLTPEQIASTLRV
                     QLEPSAETWHHFHLHPDLQIMVRDRPAGAREATYDHAFDLIVEYARTVLEDLERRVNE
                     "
     gene            1549..>1941
                     /locus_tag="VFQ54_06985"
     CDS             1549..>1941
                     /locus_tag="VFQ54_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU0164774.1"
                     /translation="MSDSERATNSSGGGIVFGGSRSDPGSGDVARGLRRRWASGVAVA
                     TAVEPNGGLRGVTVSSLMHVALDPPSLAIALATESSFHALLPEGAEFAISILDRRQEF
                     TAERFAGRAPVPDARFGGVPHDLVGGLPI"
BASE COUNT          377 a          642 c          627 g          295 t
ORIGIN      
        1 atcaccatcg gccaggcgag cgtctgcggc tcgatccggt gccggcggag ctggacgagc
       61 atgcgcagtc cccggcgggg aatccgcgcg atgagaaacc ccggcagacg acgacgccgc
      121 atgatcagcg agagaaacgt cagcatcgcg gcaacgagca ccacgaggca accagtactc
      181 gcgaacatca ggaatgtcgg gtcgccgatg aagagcgccg ggaggaggat cagcaggaag
      241 gcgacgtgac cggagacgct cttgatgaag gcggcgagca gggaatcggc cggcggcacg
      301 cccttgtgcc gcaaacgcgt gacgaagacg accaccgacg acgggccgcc gagtggagtc
      361 aacccgttga tcaccgcccg ctgcaagtgc aggctgatca tcagcgcgag gctcaaccgg
      421 tagccaagct gatggaggac gagcccgtag acggcggcga tgaggacgaa cgaaaccgcc
      481 tccaggatga ggatcgagac gatccaccgg ggatcggcgc cgacgatcag gtcccacgtc
      541 ttcctgaggt catcccgctg gttcaggccc acgtaaaccg cgaggatcag gatcgcgccg
      601 agccagaggt acgtgccgta cttcctgatg aatggcagat atcgtttgaa gcgttcgatc
      661 atgcgcgtcg cgacccggga tgcgccttta ctctcgattt cctgcacact cgggacgtca
      721 tttccttact gaatttcgag ggatcaaggg tgccgcgccg acctacgccg aggcaatgac
      781 accggccgcg atcgcaccga gggcaaagag gaacatcagg atcgcgaaca tcgtgaacga
      841 gccgcccgac tgcaccgcgg tcggcgtccg ctttacatag cgttggtaaa ggagcgacaa
      901 cccgaagcag gctgctccga ccccgattgc gaaccagagc accgcctgcc cgtccatcgt
      961 ctctcccacc atccatgcca tcgaactgcc gtatgcttcc ctccattatg aaagaaagac
     1021 cgggcggcgc gacggatgcg accgtcctgc ccgcgtcatt cggaaggaca cgcgtgagcg
     1081 acagccatcg actacctcca ggttcggtcc ctcgctacga accaccccgg cctgttcggt
     1141 atacgatcag cgatctggag cgagagaccg gcgtcaccgg acgcacgatc cgctactaca
     1201 tcgcggaggg cctgctgcag cccgcgttcg ggcgcgggcc atcggcgacc tatgattccg
     1261 accacctgct gcgactgcgc ctgatccagc agctgaagga ctcccgcctg ccgctcgccg
     1321 agatcaagga acgcctggcc cagctcacgc cggaacagat cgcctcgacg cttcgcgtgc
     1381 agctggaacc gtccgccgag acctggcatc acttccatct gcatccggac ttgcagatca
     1441 tggttcgcga ccgtcccgcc ggcgcgcggg aggccaccta cgaccacgcc ttcgacctga
     1501 tcgtcgagta cgcgcggacg gtgctcgagg acctggaacg acgcgtcaat gagtgactcc
     1561 gaacgcgcca cgaattcttc tggcggcggg atcgtcttcg gcggaagccg ttcggacccg
     1621 ggatccggcg acgtcgcccg gggactgcgg cggcgatggg cgagcggcgt agccgtggcg
     1681 acggcggtcg agccaaacgg cgggctgcgc ggcgtcacgg tcagttcgct gatgcacgtc
     1741 gcgctcgacc cgccatcgct cgcgatcgca ctggcgacgg agagctcctt ccacgcgttg
     1801 ctgccggagg gagcggagtt cgcgatctcg atcctcgatc gccgccagga gttcaccgcc
     1861 gagcgttttg ccggacgggc gccggtgccg gatgcgcgct tcggcggcgt ccctcacgat
     1921 ctggtcggag gattgccgat c
//
LOCUS       DASTMD010000480         1716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3079796, whole genome shotgun sequence.
ACCESSION   DASTMD010000480 DASTMD010000000
VERSION     DASTMD010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3079796"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..925
                     /locus_tag="VFQ54_06990"
     CDS             26..925
                     /locus_tag="VFQ54_06990"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0164775.1"
                     /translation="MKTSTMTDRATQPVVIDSHHHLWDTTKRTFGWLQDLPAINRPFL
                     PEDMRPLLAAAGVDATVLVQTIEDLDETREFLQFAADTDFIAGVVGWADLTDPAISDT
                     LRTLKDGPNGQYLVGIRHLVHNEEEPDWLLRAEVQHGLNALADAGLVYDLLVRPRELP
                     SAITVARDFPHLKMVVDHIAKPPIASGELEPWASLMREFAALEHVACKLSGMVTEADP
                     VSWTIDDLKPYVSTVVEIFGPDRMMYGSDWPVCLLAADYQQVIGAARTTLDELGVLTP
                     ASEAAIFGNTAAHWYGLDLSPYR"
     gene            1130..>1716
                     /locus_tag="VFQ54_06995"
     CDS             1130..>1716
                     /locus_tag="VFQ54_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEU0164776.1"
                     /translation="MSETTVLTDDLQTLLDAASIAPGIATRWPDYRALLIVAEGFAPT
                     GDVAYAETFLGETEARIRAMGEVDWGQHPHMAAWNEAYRGFGAKPQRTRPSALALLKR
                     VGGGLPRIDPATDLYNAISVGHVLPIGGEDIAGYEGPPRLVSATGDEPFETTDSGEAV
                     IDHPAAGEVVWRDDRGVTCRRWNWRQGTRTRITPET"
BASE COUNT          321 a          603 c          538 g          254 t
ORIGIN      
        1 caccggtgat ctcgatacat gacgtgtgaa aacaagcacc atgaccgatc gcgccacaca
       61 accagtcgtc atcgatagcc atcatcatct ctgggacacg acgaagcgaa ccttcggctg
      121 gctccaggat cttccggcga tcaatcgtcc cttcctgccg gaggatatga ggccgctcct
      181 cgcggcggcc ggcgtcgatg cgaccgttct cgtgcagacg atcgaggatc tcgacgagac
      241 ccgtgaattc ctgcagttcg ccgccgacac cgatttcatc gccggcgtcg tcggctgggc
      301 cgatctcacc gacccggcga tctcggacac attgcgaacc ctgaaggacg gtcccaacgg
      361 gcagtacctc gttggaatcc ggcatctcgt ccacaacgag gaggaaccgg actggttgct
      421 gcgggccgag gtccagcacg gactcaacgc gctcgccgac gccggactcg tctacgatct
      481 tctcgtccga ccgcgcgagc tcccgagcgc gatcaccgtc gcccgcgact tcccccacct
      541 gaagatggtc gtcgaccaca ttgcgaaacc gccgatcgcc tccggggaac tcgagccatg
      601 ggcgagcctg atgcgcgagt tcgccgcgct cgaacatgtc gcctgcaagc tctccgggat
      661 ggtgaccgaa gccgatccgg tatcgtggac gatcgacgac ctcaagccat acgtctcgac
      721 cgtcgtcgag atcttcggtc cggaccggat gatgtatggc tcggactggc cggtctgcct
      781 gctcgccgcc gactatcagc aggtgatcgg cgccgcccgc acgacgctgg acgagcttgg
      841 cgtgctgaca ccggcgagtg aggcggcgat cttcggcaac accgccgccc actggtacgg
      901 cctcgatctc tcaccctacc ggtaggcgac cgccgggcag gacaccaagc gcaccaggtc
      961 gagccgggcg accgccccgc aagacgcctt gaccgcaacg gggcaccgga cgaccaccag
     1021 ggtcgcccct acgaaggtcg gtaatcccgt agggacgacc ccggtagtcg cccttgtgtc
     1081 cgatacgcca atggcgattc gcgctgccac gcaccgaaga aagagaccga tgtccgaaac
     1141 gaccgtcctc accgatgacc tgcaaacgct cctcgatgcc gcgtcgatcg cccccgggat
     1201 cgcgacgcgc tggcccgact accgcgcgct cctgatcgtc gccgaaggat tcgcaccgac
     1261 gggcgatgtc gcctacgccg aaaccttcct cggcgagacc gaggcacgga tccgcgcgat
     1321 gggggaggtc gactgggggc aacatccaca catggcggcc tggaacgaag cgtaccgcgg
     1381 cttcggcgcg aaaccgcaac ggacgcgacc gagtgcgctc gcgctgctca agcgcgtcgg
     1441 cggcggcttg ccgcgcatcg acccggcgac cgatctctac aacgcgatct cggtcgggca
     1501 cgtcctgccg atcggcggcg aggacatcgc gggctacgaa ggaccacccc gcctcgtgag
     1561 cgccacgggc gacgaaccgt tcgagacgac cgattccggc gaggcggtga tcgaccatcc
     1621 ggcagccggc gaggtcgtct ggcgcgacga tcgcggggtg acgtgccggc gctggaactg
     1681 gcgacaggga acacggaccc ggatcacgcc agagac
//
LOCUS       DASTMD010000481         1893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2099987, whole genome shotgun sequence.
ACCESSION   DASTMD010000481 DASTMD010000000
VERSION     DASTMD010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099987"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="VFQ54_07000"
     CDS             <1..1606
                     /locus_tag="VFQ54_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU0164777.1"
                     /translation="LQMRFEAAGGYDIEHRTEAVLNGLGFTEDQYGDPVDRLSGGQKT
                     RVALAKALLESPDLLLLDEPTNHLDLEMIEWLETFLRTWNGACLIVSHDRYFLDRVTT
                     RTMDLSFGRLEDYPAPYAKYLPMREERMIRRRKEYVEQQEFIARTEEFIRKYGAGQRY
                     REARGRQKKLDRLERIERPMDHDRIHLRLGSPLRSGRMVLSSTKMTIGYNDPDGPKPL
                     IETPELLIERNERIGLIGPNGSGKTTLIKTIVDSLPALSGQYTYGTNVKPGYYAQSHE
                     QLRGKGNGTPLSVILDTGPMSEEVARNYLGRFLFSGDDVYKQVTSLSGGERSRLALGV
                     LLLEQANFLILDEPTNHLDIQARETLEEMLSDFDGTILFVSHDRYFMDRIATQLWVVE
                     DGGLSVSIGNYTDYQRQIGHRAAEIEKAEKAERSEKTPEPVRVEPEPIDVTPDSATEV
                     VITAKGKPRRKTDNDNQKALAQLEREISKLEGRLNELSDVLAIASIDEDVDAIARLGE
                     EYEKCQSELDDAYARWETVNAPVAAG"
     gene            1642..>1893
                     /gene="coaE"
                     /locus_tag="VFQ54_07005"
     CDS             1642..>1893
                     /gene="coaE"
                     /locus_tag="VFQ54_07005"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU0164778.1"
                     /translation="MSEQPRKIVLGVTGSIASGKSTVVRILKELGAIHIDADLVYREL
                     VGPGAPLLRALAEHFGEGIVAPDGSLDRAALGKIVFSDPA"
BASE COUNT          392 a          608 c          593 g          300 t
ORIGIN      
        1 cctccagatg cgtttcgagg ccgccggagg ctacgacatc gagcaccgga cggaggccgt
       61 cctcaacgga ctcggcttca ccgaggacca gtatggcgat cccgtcgatc gcctcagcgg
      121 cggtcagaag acccgcgtcg cgctcgcgaa ggcgttgctc gagagtccgg atctcctcct
      181 gctcgacgag cccacgaacc acctcgatct cgagatgatc gagtggctcg agaccttcct
      241 ccggacctgg aacggcgcct gcctgatcgt ctcccacgac cgttacttcc tcgatcgggt
      301 gacgacgcgc acgatggatc tctccttcgg tcgcctggaa gactatccag cgccatacgc
      361 gaagtacctg ccgatgcgcg aggagcgcat gatccgccgc cgcaaggaat acgtggagca
      421 gcaggagttc atcgcgagga cggaagagtt catccgcaag tacggcgcgg gtcagcgata
      481 tcgcgaggct cgcggtcgcc agaagaaact cgatcgtctc gaacgaatcg agcgaccgat
      541 ggatcacgac cggatccacc tgcgcctcgg cagcccgctg cggtccggcc ggatggtact
      601 ttcctcgacg aagatgacca tcggctacaa cgatcccgac ggcccgaagc cgctgatcga
      661 gacgcccgag ttgctgatcg aacgcaacga gcggatcggc ctgatcgggc cgaatggaag
      721 tggcaagaca acgctgatca agacgatcgt cgattcgctg ccggcactca gcggtcagta
      781 cacctacggt acgaatgtga agccaggcta ctacgcacag tcgcacgagc agctgcgcgg
      841 caagggcaac ggaacgccgc tctccgtcat tctcgatacc ggcccgatga gcgaagaggt
      901 cgcgcgcaat tatctcgggc gcttcctctt cagcggtgac gatgtctaca agcaggtgac
      961 ctcgctctcg ggcggtgaac gctcgcgact ggcgctcggt gttctcctgc tcgaacaggc
     1021 gaacttcctg atcctcgacg agcccacgaa ccacctcgac atccaggcgc gtgagacgct
     1081 ggaagagatg ctgagcgatt tcgacggcac gattctcttc gtctcgcacg accgctactt
     1141 catggatcgc attgcgacgc agctctgggt ggtcgaggac ggcggtctct cggtgtcgat
     1201 cggcaactac acggactacc agcgccagat cggccatcgc gccgccgaga tcgagaaggc
     1261 ggagaaggct gagcggtcgg agaagacgcc cgagcccgtc agggtcgaac cggaaccgat
     1321 cgatgtgaca ccggattcgg cgactgaggt cgtgatcacc gccaagggca agccacggcg
     1381 gaagaccgac aatgacaacc agaaggccct cgcgcaactc gagcgtgaaa tcagcaagct
     1441 tgaggggcga ctcaacgaac tcagcgatgt actcgcgatc gcaagcatcg atgaggatgt
     1501 cgacgcgatc gcccggctcg gcgaggagta cgagaagtgc cagtccgagc tcgatgacgc
     1561 ctacgcccgc tgggaaaccg tcaatgcgcc ggtcgcggcc ggatagccgc cgcgccagct
     1621 aacgagagac agaacgacga gatgagcgag caaccccgga agatcgtcct cggagtcacc
     1681 ggcagcattg cctcgggcaa gagcacggtc gttcgtatcc tgaaggagct cggcgcgatc
     1741 cacatcgatg ccgatctcgt ctaccgggag ctcgtcggtc ccggcgcccc cctcctccgc
     1801 gccctggccg agcacttcgg agaaggcatc gtcgcgccgg acggatcgct cgatcgcgcg
     1861 gcgttgggga agatcgtctt ctcggatccg gcg
//
LOCUS       DASTMD010000482         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_669491, whole genome shotgun sequence.
ACCESSION   DASTMD010000482 DASTMD010000000
VERSION     DASTMD010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669491"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..415)
                     /locus_tag="VFQ54_07010"
     CDS             complement(98..415)
                     /locus_tag="VFQ54_07010"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEU0164779.1"
                     /translation="MAEHFVTVARKGYLEPGELMYVEVDDEPVCLINLDGEIHALNDV
                     CTHEDASLSDGEIVGDDIECPMHGGAFNIRTGQPTSFPVVVAIEKYTVRVVGDEVQVS
                     VKK"
     gene            complement(547..951)
                     /locus_tag="VFQ54_07015"
     CDS             complement(547..951)
                     /locus_tag="VFQ54_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU0164780.1"
                     /translation="MSFGSDSIYREIILEHYHHPKNRGTLDPADFSYEDINPLCGDEV
                     RIDVRVDGDRVSEIKFSGRGCAVSQASASILTEMVEGKTLAEVKAIGKDELLDEIGIP
                     VSPARIKCALLGLKVLKAGVYGVEHAETLEGI"
     gene            complement(997..2301)
                     /locus_tag="VFQ54_07020"
     CDS             complement(997..2301)
                     /locus_tag="VFQ54_07020"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU0164781.1"
                     /translation="MALANSTTSLDAFRLKGDFPILSQPQTNGRAPLVFLDSAASSQK
                     PQVVIDALADYYTSYNANIHRGVYHLSELATTAYEDARRKVARFINAKSARECIFVRN
                     TTEAINLVAQSWGRANLNEGDIILFTEMEHHSNIVPWHLIAEQRGAKVDYVPVTPDGL
                     LDQDVYEEKLKLGPKVVAFTHGSNVLGTVNPVKEMIARAHAAGAVTVLDGAQSVPHFA
                     VDVQDLDCDFMGFSGHKMLGPMGGGILYGKRALLDAIPPYMGGGSMIRKVELTHTTYA
                     DLPAKFEAGTPAVADAIGLGVAIDYLNGIGMDRLHAHELEMTRYAHERLQEVPGLTLF
                     GPRDFDVLSGVASFALGDLHPHDVAQVLDEHNVAVRAGHHCAQPLMRALDVVATTRAS
                     FYLYNTTDDIDRLVEGLYDAYRTMNGGVSPTISDARSVNAAG"
     gene            complement(2445..3812)
                     /gene="sufD"
                     /locus_tag="VFQ54_07025"
     CDS             complement(2445..3812)
                     /gene="sufD"
                     /locus_tag="VFQ54_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU0164782.1"
                     /translation="MDVSTKTSSVAAADLTGFTREGVEALSRAKNEPAWVLEARLAAW
                     EIYDALPMPTRQDEEWRRTDLRRLKLDQYAPFAGTDQRVSALGDVLHAEAEEHALQFG
                     EADQRAGIIVNQDSGTAYTELDPTVAAQGAIFVDLDTAIAEYPELVKQYFMTEAVPVS
                     FGKFQALHAAFWQTGSLLYVPKGVSITLPFRQFVQASAPGASTFSHSLVVIEEGAEAF
                     FVDAFQSATQAEPAFTSHVVELIVRADAKLRYVQVQDWGRHVFNFMTERSVLGRDTEL
                     KSLQVTLGSKFTKNSIGSHLRGENSLAEMLGIFFGDEDQFFDHHTWQLHESPYATSDL
                     EFKGAMKERSRSVYSGLIKVSEGAQRTDAYQQNRNLVLSREARADSIPNLEISANDVR
                     CTHGATVSQVEDEHIFYLQARGIPRSEAQKLIVEGFFRPVIDRIPVEEIQDFLQGAIA
                     AKVGY"
BASE COUNT          729 a         1258 c         1211 g          752 t
ORIGIN      
        1 ttatcggaat ctcccttcgg atcagctgcg gtgcttcccg gtgcgcccgt tcgttcgtgg
       61 ggcgggacgc cccccgacgc cggagccggg tcatgcgcta cttcttgacc gagacctgaa
      121 cctcatcgcc gacgacgcgg accgtgtact tctcgatcgc gaccacgacc gggaacgacg
      181 ttggctggcc ggtgcggata ttgaatgccc cgccgtgcat cggacattcg atgtcgtcac
      241 cgacgatctc gccatccgaa agcgacgcgt cctcatgggt acagacgtcg ttcagcgcat
      301 ggatctcgcc gtcgagattg atcaggcaaa ccggctcgtc atcgacttcg acatacatca
      361 actcgccagg ctcgagatag cccttcctgg cgaccgtcac gaaatgctcc gccattcaac
      421 gtccctcgca atacacagat tagatcgaat ctcattatcg ttcagactga cgcctgagaa
      481 tgaaaacgcc cgggcgagaa tggcatcccc gcccgagcag cccgatcgag caaccgcgtc
      541 gggaacctag ataccttcga gggtttcggc gtgctcgacg ccgtagacac ccgccttgag
      601 caccttcaga ccgagcagcg cgcacttgat gcgcgcgggg ctgaccggaa tcccgatctc
      661 gtcgagaagt tcatccttcc cgattgcctt cacttcggcg agggtcttcc cttcgaccat
      721 ctcggtcagg atcgaggccg aggcctggct gaccgcgcag ccgcggcccg agaacttgat
      781 ctcggagaca cgatcgccat cgacacgaac gtcgatccga acctcatccc cgcagagggg
      841 attgatgtcc tcgtacgaga aatccgccgg atcgagcgtg ccccgattct tcgggtgatg
      901 gtaatgctcg aggatgattt ccctgtagat gctgtcgctg ccgaaactca tctcatgttc
      961 ccgttcttct ggatcccgcg gtcacgcccg gacccgctag cctgccgcat tgaccgaccg
     1021 ggcatcggag attgtcggcg ataccccacc gttcatcgtg cgataggcat cgtacaaccc
     1081 ttcgaccagg cgatcgatat cgtccgtcgt gttgtagagg tagaaactgg cccgcgtcgt
     1141 cgcgacgaca tcgagggccc gcatcaatgg ctgcgcgcag tgatgaccgg cacggacggc
     1201 gacgttgtgc tcgtccagca cctgggcaac atcgtgcggg tgaagatcac cgagcgcgaa
     1261 cgaggcgacg ccgctcagga catcgaagtc acgcggaccg aagagggtca ggccggggac
     1321 ttcctgcagc cgctcgtgcg cgtatcgcgt catctcgagc tcgtgcgcat gcagccggtc
     1381 cataccgatg ccgttcagat agtcgattgc aaccccgagt ccgatcgcat cggcgacggc
     1441 cggagtgccg gcctcgaact tcgccggaag atcggcatac gtcgtgtgcg tcagctcgac
     1501 cttgcggatc atgctgccgc ctcccatgta gggcgggatc gcatcgagga gcgcacgctt
     1561 gccgtagagg atgccaccac ccattggccc cagcatcttg tgccccgaga agcccatgaa
     1621 gtcgcaatcg agatcctgca cgtcgaccgc gaagtgggga acgctctgcg cgccgtcgag
     1681 gacggtcacc gcgccggcgg catgcgccct ggcgatcatc tccttgaccg gattgaccgt
     1741 gccgaggacg ttactgccgt gggtgaaggc gacgaccttc ggtcccagct tcagcttctc
     1801 ttcgtagaca tcctgatcca gcaagccgtc cggcgtcacc ggcacatagt cgaccttcgc
     1861 gccacgctgc tcggcgatca ggtgccaggg gacgatattg ctgtgatgct ccatctcggt
     1921 gaagaggatg atgtcccctt cgttgagatt ggctcgtccc caggactgcg cgacgaggtt
     1981 gatcgcctcg gtcgtgttcc ggacaaagat gcattcccgc gccgacttcg cgttgatgaa
     2041 gcgcgccacc ttgcggcggg catcctcata ggcagtcgtc gccagctcgg agagatggta
     2101 gacaccgcgg tggatgttgg cgttgtagga cgtgtagtag tcggcgagcg cgtcgatcac
     2161 gacctgcggc ttctgcgagc tcgcggcgct gtcgaggaag acgagtggcg cacgaccatt
     2221 cgtctgtggt tgcgaaagaa tcgggaagtc gcccttcagc ctgaaggcat caagcgaggt
     2281 cgtcgagttt gcgagggcca tttgcagctc ctgggattca acgtggcgat ggcttccatc
     2341 accgagcatt gtccggctgc cgggcatgga acaaggacac gacctgggtt ggatatggag
     2401 acgggtgaca ccgtgtgtgc cacccgtccc gctggatcga cgtcttaata gccgaccttc
     2461 gccgcgatcg cgccctggag gaaatcctgg atctcctcca ccggaatgcg atcgatcacc
     2521 ggccggaaga agccttcgac gatcagcttc tgcgcctccg atcgcggaat gccgcgcgcc
     2581 tggaggtaga agatatgctc gtcctcgacc tgcgagaccg tcgcaccgtg ggtgcaacga
     2641 acgtcattcg cgctgatctc gagattcggg atcgaatccg cccgcgcctc gcggctgagg
     2701 acgagattgc ggttctgctg gtaggcgtcg gtgcgctggg cgccctcgga gaccttgatc
     2761 agtcccgaat agacggagcg cgaacgctcc ttcatcgcac ccttgaactc gagatcgctc
     2821 gtcgcgtacg gcgactcgtg cagctgccag gtgtggtgat cgaagaactg atcctcgtcc
     2881 ccgaagaaga taccaagcat ctctgcaagc gagttctcgc cgcggaggtg ggatccgatc
     2941 gagttcttgg tgaacttcga gccaagcgtg acctgcagcg acttcagctc ggtatcgcgt
     3001 ccaagcacgg aacgctcggt catgaagttg aagacgtgac gtccccagtc ctgaacctgc
     3061 acgtagcgca gcttcgcgtc ggcacgaacg atgagttcca cgacgtgcga ggtgaacgcg
     3121 ggttcggcct gcgtcgcgga ctggaatgca tcgacgaaga acgcctccgc gccttcctcg
     3181 atcacgacca gcgagtgcga gaaggtcgac gcccctggcg ccgacgcctg cacgaactgc
     3241 cggaacggca gggtgatgct gacacccttc ggcacgtaga gcagcgaacc ggtctgccag
     3301 aacgccgcat gcaacgcctg gaacttgccg aacgagaccg gcacggcctc tgtcatgaag
     3361 tactgcttga cgagctccgg gtactccgcg atcgcggtgt cgagatcgac gaagatcgcg
     3421 ccctgcgccg cgacggtcgg atcgagttcc gtgtatgcag tgccgctgtc ctgattgacg
     3481 atgatgcccg cgcgctggtc ggcttcgccg aactgcagcg cgtgctcttc cgcctccgcg
     3541 tgcaacacgt caccaagcgc gctcacacgc tggtcggtgc cggcaaacgg agcgtactga
     3601 tcgagcttga gacgacgaag atcggtccgc cgccactcct cgtcctggcg cgtcggcatc
     3661 ggcagcgcgt cgtagatttc ccacgccgcg agtcgcgcct cgagcaccca ggcgggctcg
     3721 ttcttggcgc gggagagcgc ttcgacgccc tcccgcgtga atccggtcag atccgccgca
     3781 gcgactgacg atgtcttggt tgatacgtcc attgctacct caacgaatgt ccggtcggtg
     3841 ccgggggtcg tcgttatccg accgaacctt ccatctcgag ctggatcaac cggttgagct
     3901 cgaccgcgta ttccagcggg agttccttgg cgatcggctc gatgaagcca
//
LOCUS       DASTMD010000483         1764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3170766, whole genome shotgun sequence.
ACCESSION   DASTMD010000483 DASTMD010000000
VERSION     DASTMD010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3170766"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1495
                     /locus_tag="VFQ54_07030"
     CDS             11..1495
                     /locus_tag="VFQ54_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU0164783.1"
                     /translation="MNDKLAVFAPFVKSYDLTSVAAGGSSYRHGSMVTSALLYGSIDP
                     AAPLPDPTANVDHYRVFPLPPLKPGEPDHRLYDVLDQMKATLTRQHYPLVVLAIGPND
                     SLDENAEPDRFTVELDTLAFENGITFITAVGNNGELDTHLGYDRVQPPGDMVNGIGVG
                     AITRKHRDPKKRWKRAPYSARGPGREGQRVQPLIVEFGGSNDERFIGFDERGRLTSAM
                     GTSFASPTTGRAAGGLQAILDRARHRPDIFRAFAVHHAERGHGHGKHYCELGYGRVLA
                     DFNEALECDANEVTVLFEDELPRAEPIAHRFPLPKGLPPATRLELNWTVSYISEVDPR
                     DATDYTLSGIEAIFRPDSRKRTLTDPATNKGEAVCLQADHARVTAAITAGWRLSEEPT
                     AHSSWALTKGEQKLRREGKWETIMPGRVLLAAGDLYEPRLDLTHIRRENGRLVRGNAI
                     KPLRFSMLLTIRTRDGEAIYDQIAAQYRVLTPLVRVPLRVSTVA"
     gene            1507..>1764
                     /locus_tag="VFQ54_07035"
     CDS             1507..>1764
                     /locus_tag="VFQ54_07035"
                     /inference="COORDINATES: protein motif:HMM:NF037930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164784.1"
                     /translation="MAISLVHELQRLAASGDTPPTEVLRMAKVVAAKLNIDDARSWID
                     HETNGYPDAALLPSYRIIPCELRGHNRVRGWQQVRWDVANQL"
BASE COUNT          330 a          574 c          555 g          305 t
ORIGIN      
        1 cgacggtggt gtgaacgaca agctcgcggt gttcgccccg ttcgtgaagt catacgattt
       61 gacgtccgtt gcagcgggcg gttcgtcgta caggcacgga agcatggtga cgagcgcact
      121 gctctacggg tcgatcgatc cggcggcacc actgcccgac cctaccgcga acgtcgatca
      181 ctaccgcgtc tttcccctgc cgcccctcaa gccgggagag cccgatcaca gactttacga
      241 cgtgctcgac cagatgaagg cgaccttgac gaggcagcac tatccgctcg tcgtgcttgc
      301 gatcggcccc aacgactcac tcgacgagaa cgccgagccc gatcggttca ccgtggaact
      361 cgacacgctg gccttcgaga acggcatcac gttcatcacg gcggtgggta acaacggcga
      421 gttggataca catctcggct acgatcgcgt ccagccgcct ggcgacatgg ttaacggcat
      481 cggcgtcggt gccatcacgc gcaagcatcg cgaccccaag aagcgttgga agcgtgcgcc
      541 gtactccgcg cgtggccctg gtcgagaggg ccagcgtgtc cagcccctca tcgtcgaatt
      601 cggcggtagt aatgacgagc ggttcattgg gttcgatgaa cgcggtcggc tcacgtctgc
      661 tatgggcacg agtttcgcct cgccgacgac gggtcgtgcc gccggcggac tgcaagccat
      721 cctcgaccgc gcgcggcacc gtcctgacat tttccgtgca ttcgcggtcc accacgccga
      781 acgcggacat ggccacggca agcactactg cgagttgggg tacgggcggg tgttggccga
      841 tttcaacgag gcgttggagt gcgacgcgaa cgaggtaacg gtgctgttcg aggacgagct
      901 gccacgcgcg gagccgatcg cacaccgctt cccgttaccg aagggactgc ctcccgcgac
      961 gaggctcgag ttgaattgga cggtgagcta catttcggaa gtcgacccac gcgacgcgac
     1021 ggattacacg ctgtcgggca tcgaggccat ttttcgcccg gattcgcgca agcgcacgct
     1081 cacggatccg gcgacgaaca agggcgaagc ggtgtgtctc caagcggacc acgcacgtgt
     1141 aaccgccgcg atcaccgccg gctggcgatt gtcagaggag cccaccgccc attcgagttg
     1201 ggcgctcacc aagggcgagc aaaaactccg ccgcgaaggc aagtgggaga cgatcatgcc
     1261 ggggcgcgtc ttgctcgcgg cgggggatct ctatgagccc cgcctcgatc tcacgcacat
     1321 ccgcagggag aacggccgcc tcgtgcgggg caacgcgatc aagccgctgc ggttcagcat
     1381 gctgctgacg atccgcacgc gcgacggcga agcgatctac gatcaaatcg ccgcccagta
     1441 tcgcgtgctc accccgctcg tgcgcgtccc gcttcgtgtg tcaaccgtcg cgtgaaagcc
     1501 gcagaaatgg ctatttccct cgtccatgag ttgcagcgcc tcgctgcttc cggcgacacg
     1561 ccccccactg aggtgctgcg tatggccaag gtcgtcgcgg ccaagctgaa catcgatgac
     1621 gcccgtagct ggattgatca cgaaaccaac ggatatccag acgcggcttt acttccctct
     1681 taccggatca tcccgtgcga gctgcgtggc cataaccgcg tgcgcggatg gcagcaggtg
     1741 cggtgggacg tggcgaacca gctc
//
LOCUS       DASTMD010000484         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_132728, whole genome shotgun sequence.
ACCESSION   DASTMD010000484 DASTMD010000000
VERSION     DASTMD010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132728"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(354..716)
                     /locus_tag="VFQ54_07040"
     CDS             complement(354..716)
                     /locus_tag="VFQ54_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0164785.1"
                     /translation="MTETTTRPPVITEYIAAMNAFDGDRLLAVFADDALINDDHREFW
                     GKAAIKPFLDEEFIAAKVTMEVPRVIAHHGQYIIPAIVDGEYDKTGLPDPLILTNYIT
                     VEGDRIVTLIIIHNHARA"
     gene            complement(827..1669)
                     /locus_tag="VFQ54_07045"
     CDS             complement(827..1669)
                     /locus_tag="VFQ54_07045"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU0164786.1"
                     /translation="MKSVPHALTIGDFSRTTHLTVKTLRHYHRVGLLEPEAINPETGY
                     RYYGTDQVPVAQVIRRLRDLEMPIDDVRAVLAAPDITARNTLIAAHLERLERQLQATA
                     AAMTSLRNLIDHPHAPDDAATAIQHRSIAATRSLAIREIVPRGGLGAWWPQALAALRT
                     FVETERIAQAGPAGGLFTNGLFTDEEGEAALFLPVEGTIEGRGRIRPFLVPAAEVAVV
                     AHHGSHADIDLAYGALGTYVARYALGIEGPLREYYVIDASDTPDSSQWVTEICWPVFQ
                     TTDR"
     gene            1843..2187
                     /locus_tag="VFQ54_07050"
     CDS             1843..2187
                     /locus_tag="VFQ54_07050"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0164787.1"
                     /translation="MKYVLMTVHNDDVWAGLSQAEKEATWRDGGAWWDTLAGSGEGIF
                     GAPFADVSETHLVRTQDGTTVTTKGPLTDSKDQIAGYVLLDVASEQRALELAAEWPES
                     RHATIVVRQLMG"
     gene            2239..2592
                     /locus_tag="VFQ54_07055"
     CDS             2239..2592
                     /locus_tag="VFQ54_07055"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0164788.1"
                     /translation="MLMIYHNAELWAALAEKEQQALMADGGRRWRELVATGEAIMGEP
                     LADGAGARAVRVRDDAVVVTDGPFVEAKEQFVGFLLVDVATRERAVELATQWPDARVG
                     TIYVREIRVVNELNP"
     gene            2646..2996
                     /locus_tag="VFQ54_07060"
     CDS             2646..2996
                     /locus_tag="VFQ54_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0164789.1"
                     /translation="MMLIYQNTEAWNALAEEERQAMMEEAGEIWGNLMATGEVVSGEA
                     LAAPTLARTIRVREGVSMVTDGPFIEAKEQLAGLVVFDVASEERAIEIATGWPDARYW
                     AVELRPLMHQGGEA"
     gene            3014..3883
                     /locus_tag="VFQ54_07065"
     CDS             3014..3883
                     /locus_tag="VFQ54_07065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU0164790.1"
                     /translation="MPGMPRIGIGLPNFGPEAGPEAILRIASTVERLGFHGVSVCDRL
                     LLPLGEEWENTYGLPDSYIFDPVETLTFVAARTGRIRLATTIVNSPFQSPIVLARRLA
                     TLDQLSGGRLDAGIGLGWMPEEYAASGVPYAERGARFEDHLAAMRACLGPDPVQHDGP
                     FYQVPKAKIGPKPVNGRLCVLVGGVAKPAIERAARLGDGFIAAIREWETTLQEIAWYR
                     AAGGTGMVMLRTASSWDAQAEDPIGMSVAAMMADLTTFAEAGVDEVHWDLTLAGIGID
                     RQIEVLEALAMHV"
BASE COUNT          698 a         1180 c         1460 g          714 t
ORIGIN      
        1 tcggcgaccc gccgcagagc atggtcatcc atgacgcggt cgtgagtagg gggggacttc
       61 tatgtccccc ctgaagcaag gcgccgtgcc gcacagcgct ctctcagaca cgagtcggcg
      121 tcgccgaccc gatgcgacca caagggtcgc gtctacggtt cattgaacga tgaggatatc
      181 gagacgcgtc cacgcagcga tcgcaaggtg cgttctttga tgcagcgttc gtgcgtaggg
      241 aagctctgca gtcgtcggac cggttgcggc gaatcgttgc atgccgaatc cgaggtccga
      301 tgacggaatg tgcttccttc ggctccggcg ccccagcgct tgcattcccg ggttcaggcc
      361 cgggcgtggt tgtggatgat gatcagcgtg acgatcctgt cgccttcgac ggtgatgtag
      421 ttcgtgagga tgagggggtc ggggagaccc gtcttgtcgt attcgccatc gacgatcgcc
      481 gggatgatgt attgcccgtg atgcgcgatg acccggggca cctccatcgt cactttcgcg
      541 gcgatgaact cctcgtcgag gaagggcttg atcgccgcct tgccccagaa ctcgcgatga
      601 tcatcgttga tgagggcgtc gtcggcgaag acggcaagca ggcggtcgcc atcgaacgcg
      661 ttcatggcgg caatgtattc cgtgatgacg ggaggacgtg ttgtggtctc ggtcatgatc
      721 tgctccgtgg ttgggacgtg tatcgaatgc cagattcgct ggcaaacacc acgataggac
      781 ctccccttgg gggagggtca agcagtgcgc cgggtgtcga cgggagtcaa cggtcggtcg
      841 tctggaagac cggccagcag atctcggtca cccattgtga ggagtccggc gtatcggacg
      901 catcgatgac gtagtactcg cgcagcggac cctcgatccc cagtgcatag cgggcgacgt
      961 aggtccccag ggcgccgtag gcgagatcga tgtccgcgtg cgatccgtga tgggcgacga
     1021 cggcgacctc ggcggccggc acgaggaacg gccggatcct gccgcgtcct tcgatcgttc
     1081 cctcgaccgg aaggaagagg gcagcctccc cctcctcgtc ggtgaaaagg ccgttcgtga
     1141 agagaccacc cgcgggtccg gcctgggcga tccgctccgt ctctacaaat gtgcggagcg
     1201 cggccagtgc ctggggccac caggcaccga ggccgccacg cgggacgatt tcccggatcg
     1261 cgagtgaccg ggtggcggcg atcgatcggt gctggatcgc ggtcgcggcg tcatccgggg
     1321 cgtggggatg gtcgatcagg ttgcgcagcg acgtcattgc cgccgccgtc gcctgcaact
     1381 ggcgttccag acgctcgagg tgggccgcga tcagggtgtt gcgggcggtt atatccgggg
     1441 cagcgaggac cgcgcggaca tcatcgatcg gcatctcgag gtcgcgcagg cgccggatca
     1501 cctgggcgac cggcacctgg tcggtcccgt agtagcggta gccggtctcg ggattgatcg
     1561 cctccggttc cagcagaccg acgcggtgat agtggcggag ggtcttcacc gtgagatggg
     1621 tggtgcgcga gaaatcaccg attgtcaggg cgtggggaac gctcttcatg ggctgtccgg
     1681 ttcgtgggaa tgggcctggt gcgacgtatc gcgatcgatc atacatgacc gagcgaaggc
     1741 gggcagaatc attgacggac cgatgtcgag aacccggatt cggctccgac taacgggaga
     1801 agggcactca ggatgggtgt cgacgtccag aggagaacca ccgtgaagta cgtactgatg
     1861 accgttcaca atgacgatgt ctgggccggg ctttcgcagg ccgagaagga agcgacctgg
     1921 cgcgatggcg gagcctggtg ggatacgctc gccgggagtg gcgaaggaat tttcggcgca
     1981 cccttcgccg acgtctcgga gacgcatctc gtgcggacgc aggatggcac gacggtgacg
     2041 acgaaggggc cgctgaccga ttcgaaggat cagatcgccg ggtacgtgtt gctcgatgtc
     2101 gccagcgagc agcgcgcact cgaactcgcg gcggaatggc cggagtcccg ccacgcgacg
     2161 atcgtcgttc gtcagctcat gggctgaccg cccggattcg acctccgcgt gatacgtgag
     2221 gagcgccagt tgaagtacat gctgatgatc tatcacaacg ccgaactctg ggctgcgctg
     2281 gccgagaagg aacagcaggc attgatggcc gacggcggtc gtcgctggcg ggagctcgtc
     2341 gcgacgggcg aggcgatcat gggggagccg ctcgcggacg gagcgggggc tcgtgccgtc
     2401 cgcgtccgcg atgacgccgt cgtggtgacg gatggtccgt tcgtcgaggc gaaggagcag
     2461 ttcgtcggct tcctgctcgt cgatgtcgcg accagggaac gcgccgtcga gctcgcgacg
     2521 caatggccgg acgcccgcgt cgggacgatc tacgtccgcg agatccgggt cgtcaacgaa
     2581 ctcaatccat aaccggggcc agtctccaac ggcgccgaac cagcaggagt cacgacgtga
     2641 agtacatgat gctgatctac cagaacaccg aagcgtggaa cgcgctggcc gaggaagagc
     2701 ggcaggcgat gatggaggaa gccggcgaga tctgggggaa cctgatggcg accggtgagg
     2761 tggtgagtgg cgaggcgctc gcggctccca cgcttgcgcg caccatccgc gtccgcgaag
     2821 gggtttccat ggtgacggat gggccgttca tcgaggcgaa ggagcagctc gccgggctgg
     2881 tcgtgttcga tgttgccagc gaggagcggg cgatcgagat cgcgaccggg tggccggatg
     2941 ctcgctactg ggcagtcgag ctgcgaccgc tgatgcatca gggtggcgag gcctagccgg
     3001 ggaggaatcg tttatgccag gcatgccacg gattggcatt ggactcccga atttcggacc
     3061 cgaggcggga ccggaggcga tcctcaggat cgcctccacc gtcgagcggc tcggtttcca
     3121 tggcgtctcc gtctgcgacc ggctcctgct gccgctcggt gaagaatggg agaacaccta
     3181 cggtcttcct gacagctaca tcttcgaccc ggtcgagacg ctgacattcg tcgccgcgcg
     3241 caccggacgg atccggcttg cgacgacgat cgtcaactcg cccttccagt cgccgatcgt
     3301 gctggcgcgg cggctggcga cgctcgacca gctctccggt gggcgactcg atgccggcat
     3361 cggtctcggt tggatgccgg aggagtatgc ggcctccggc gtgccgtacg ccgaacgcgg
     3421 cgcgcgcttc gaggatcatc tcgcggcgat gcgtgcctgt ctcggaccag atccggtcca
     3481 gcacgacggc cccttctacc aggttccgaa ggcgaagatc ggtcccaaac cggtgaacgg
     3541 gcggctatgc gtgctggtcg gtggggtcgc gaaaccggcg atcgagcgcg cggcgcgcct
     3601 gggcgacggc ttcatcgcgg cgatccggga gtgggagacg acgctccagg agatcgcgtg
     3661 gtaccgcgcg gccggtggca cgggcatggt gatgctccgc accgcatcgt cctgggatgc
     3721 gcaagcagag gatccgatcg gcatgtcggt tgcggcgatg atggcggatc tcacgacgtt
     3781 cgcggaggcc ggtgtcgacg aggtgcactg ggatctgacg ctggccggga tcgggatcga
     3841 tcgccagatc gaggtgctcg aagcgctcgc catgcacgtc tgacctggat cagacggcgt
     3901 cgacgggtgg gcgaccggtg aatttggcga tgagccactc cggattcgga aagaagtgat
     3961 gttcccggac ggcacggaca acagcttcgg tgtcatattg gactcgtcgg ggtgtcacct
     4021 ggtatccgtt gatatccgcc tcgaggaggg cg
//
LOCUS       DASTMD010000485         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_669835, whole genome shotgun sequence.
ACCESSION   DASTMD010000485 DASTMD010000000
VERSION     DASTMD010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669835"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFQ54_07070"
     CDS             complement(<1..295)
                     /locus_tag="VFQ54_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164791.1"
                     /translation="MTGDSPKRPSGARRLLRTTGAAILACFLALGFIAGSSEVQFAAA
                     QSVIDPDIDSDGDNINNRWDPDDDNDGYPDEIDPDPFDASNPAYVPTPNTIDPD"
     gene            567..1838
                     /locus_tag="VFQ54_07075"
     CDS             567..1838
                     /locus_tag="VFQ54_07075"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164792.1"
                     /translation="MRVAAKDSIWRSADFRRLWMAQTVSEIGSQVSFLALPLVATMVL
                     QASALEMGLLTAAGALPSLLLGLHAGAMIDRRRKRPLMVMSDVGRAALLAVIPLAWLC
                     GVLDFELLILVALLGGVFTLVFQVASQSFLPLVLVRGKLVDGNSKLELSRSVAEVVGP
                     GLGGWLISLLTAPLSLLVDASSYLCSALFLSGIQVPESNPAVGEGRVPWRREIGDGMR
                     AVLAEPRLRVAAGAGSLLEFFNAMLEAVFVLFLVRELGLKPGLIGLVFMIGSVGFVVG
                     AMAPDYLTRRFGLGRMTTLSVLLIGVSDMIVPFAYGAVWMVLSLMVVAQLTFGIGLTI
                     FRVNRSSLLQALIPASMRARATATMRTLTMSLVPVGALAGGLLGTWAGIRETLVIAAT
                     GEIVAALWIWHSPLREMRAIPTADGIPEISA"
BASE COUNT          318 a          610 c          676 g          449 t
ORIGIN      
        1 cgtcgggatc gatcgtgttc ggggtcggca cgtaggcggg attggaggca tcgaagggat
       61 cggggtcgat ctcgtcggga tacccgtcgt tatcgtcgtc cggatcccac cggttgttga
      121 tgttgtcgcc gtcgctatcg atgtcgggat cgatcacgct ctgggcggcg gcaaactgga
      181 cctcgctgga tcccgcgatg aacccgagcg ccaggaaaca ggcaaggatg gccgcgcccg
      241 tcgttcgcag gaggcgtctg gcaccggacg gccgtttcgg agaatcccct gtcaccacgt
      301 tcacactcca ccacgttcgg agtctcacga ctcccagttg cggacgcaca tggttccgtg
      361 gttcgcgcgc cctcgctccg ttgtttcatg accctgacgt catagttacg tgaaggcgag
      421 tcgctcgtca accagtacgg acatgttttc gtggagtgac gatcaccgcg tacgggttag
      481 cactcggcat atagagagtg ctaatgttga ggtcttatga ccttcccatg agccgacgca
      541 ggcgggcgag caggggacga gagaagatgc gtgtcgcggc aaaggactct atctggcgta
      601 gtgcggactt tcgccggttg tggatggcgc agacggtgtc ggaaatcggc tcacaggtaa
      661 gcttcctggc gctgccgctc gtcgcgacga tggtgcttca ggcatcggcg ctggagatgg
      721 gactccttac cgccgccggc gcgctcccgt cattgctgct cggactgcac gccggggcga
      781 tgatcgatcg gcgacgaaag cgcccactga tggtgatgtc tgacgtgggg agagctgcac
      841 tgcttgcggt cattccgcta gcctggctgt gcggggtcct cgacttcgaa ttgctgatcc
      901 tcgttgcgct gctcggcggg gtgttcacgc tcgtcttcca ggtcgcatcc cagtcgttcc
      961 tgccccttgt cctcgtacgg ggaaagctcg tcgatgggaa cagcaagctt gaattgagcc
     1021 gatccgtggc ggaggtcgtc ggcccgggcc tcggcggatg gctcatctcc ttgttgacgg
     1081 caccactctc gctgcttgtc gatgcctcct cctatctttg ctcggcgctc ttcctgagcg
     1141 gtatccaggt tcccgaatcg aatccggcag tgggcgaagg tcgcgtcccc tggcgtcggg
     1201 agattggcga cggtatgcgt gcggtgctgg cggagccgag attgcgggtc gccgccggcg
     1261 ccggatccct gctcgaattc ttcaatgcga tgctcgaggc cgtcttcgtc ctcttcctcg
     1321 tccgggaact cggactgaag ccgggactga ttggcctggt cttcatgatt gggagcgttg
     1381 gcttcgtcgt cggggcaatg gcacccgatt atctgacgcg acggtttggt ctcggccgga
     1441 tgacgacgct ctcggtgctg ctgatcggcg tgagtgacat gatcgtgccc ttcgcctatg
     1501 gggcggtctg gatggtgctg tcgctgatgg tggtcgcgca gctcaccttc ggcattggcc
     1561 tgaccatttt ccgggtcaac cgatcgagcc ttctccaggc gttgatacca gcttcgatgc
     1621 gcgcacgggc aacggcgacg atgcggacac tcaccatgtc cctcgttccc gtcggtgccc
     1681 ttgcgggtgg actccttgga acctgggccg ggattcggga gaccctcgtc atcgcggcga
     1741 ctggggagat tgtggctgct ctctggatct ggcactcgcc gctccgcgag atgcgcgcga
     1801 tccccaccgc ggatgggatc cccgagatct cagcctgatc gcatcgtggt tgatacgaaa
     1861 ccggccccag ggtgaaatct caccgcgggg ccagtcacat tccgcccaag agaggacgcg
     1921 agcgatcagt tgtggttgag cggaaccgcc gccgtcaccg tcgtgtacgg gtagtccgaa
     1981 tcgatatcga gctcgtaact cgcaccgtcg agggtgtaac ccgcgggaag actcgtgact
     2041 ttcacggttc gct
//
LOCUS       DASTMD010000486         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1117263, whole genome shotgun sequence.
ACCESSION   DASTMD010000486 DASTMD010000000
VERSION     DASTMD010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117263"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFQ54_07080"
     CDS             complement(<1..299)
                     /locus_tag="VFQ54_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HEU0164793.1"
                     /translation="MTKQDVSPRDQLPPLIAIVGPTAVGKTAASIRIATAISAEIINA
                     DSRSFYRGMDIGTAKASPSERAEVPHHLIDILEPTDPMSLATFQDLVYEIVAGIH"
     gene            441..1604
                     /gene="mltG"
                     /locus_tag="VFQ54_07085"
     CDS             441..1604
                     /gene="mltG"
                     /locus_tag="VFQ54_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU0164794.1"
                     /translation="MIRVVTQSLKIVSILVVAIAVVAGSVAFFNYWTDRTSSAEVGRP
                     VTIEITADDDTGSVAKKLTKAKLVKYGFYFEIRMRLGGGDLQPGVYTIPKGDSVSEII
                     KRITVKSSSSDSADTSSSKPPTAIKMTFIEGQRIGQFADQFNNAGFSWGSADFIKAAS
                     NPANRKGFDFLSGLPADASLEGFLFPDTYTFGSNATPDQVVQLMLEDFQTRFDASMLK
                     EQKSSGLTMLQVVTIASIVEREAAVPEERPIIAKVYINRVNDGMQLNADPTLSYMAGK
                     EGDWWPKITDAILAKESPYNTYKVVGLPPGPIASPSLASLQAVLQPADVTYLYFVAKN
                     DGSQTHAFADTLDEQNANTCTYQGTGCDGSSGGDGTGSSGDTPTATVPPDDGN"
     gene            1640..>2356
                     /locus_tag="VFQ54_07090"
     CDS             1640..>2356
                     /locus_tag="VFQ54_07090"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU0164795.1"
                     /translation="MVAYVLRAGLIGNPVAHSRSPRMHNAAFAALGVDAHYELWLTEV
                     EEIPDRIATVRGAGMLGANVTVPHKQAVMPFCDRLSETAQRAGAVNTLIPTADGLLGE
                     NTDVHGFAESVREAIGRDGDVPPCALLLGAGGAARGVVVALQQLGVGHITIANRTLAR
                     AQEIADALAGDIGTIDAIPLDRLGDAAPGVGFLINSTSVGWHGDELPVPESFIEALPA
                     STLVANLTYRQTALLRIAGQR"
BASE COUNT          442 a          742 c          749 g          423 t
ORIGIN      
        1 tcgtgaatcc cggcaacgat ctcgtacacc agatcctgga aggtcgcaag cgacatcgga
       61 tcggtcggtt cgaggatatc gatcagatga tgcgggactt cggcccgttc cgaaggcgac
      121 gccttggcgg tgccgatatc catgccccgg tagaacgatc gggaatcggc attgatgatc
      181 tccgccgaga tcgcggtcgc gatccggatc gaggccgcgg tcttgccgac ggcggtcggg
      241 ccgacgatcg caatcagcgg aggtagttgg tcacggggcg aaacgtcctg tttggtcatc
      301 gtagaactat ccgactagaa tgggcgggtg ccggtcgccc ggcttcgatt acctggcgcc
      361 gtccatacgc acaccccggt tgctgcatat cgcgcgatcg cattcttccc acgcagagcc
      421 agggttcagg agtatgtcgc ttgattcgtg tcgtcactca atcactgaag atcgtctcga
      481 ttctcgtcgt ggcgatcgcg gtcgtcgcgg gcagcgtcgc cttcttcaac tactggaccg
      541 atcgcacctc ttcggccgag gtcgggcgcc cggtcacgat cgagattacc gccgatgatg
      601 ataccggaag cgtcgccaag aagctgacca aggccaagct cgtcaagtat ggcttctact
      661 tcgagatccg gatgcgcctc ggtggcggcg acctccagcc cggcgtctac acgatcccca
      721 agggtgacag cgtctccgag atcatcaagc ggattacggt caagagcagt tcgtcggaca
      781 gcgccgacac ctcctccagc aaaccgccga ccgcgatcaa gatgaccttc atcgaagggc
      841 aacgcatcgg gcagtttgcc gatcagttca acaatgccgg cttttcatgg ggaagcgccg
      901 acttcatcaa ggccgcgtcc aaccccgcca accggaaggg attcgacttc ctcagcggac
      961 tccccgccga cgccagcctc gagggattcc tgttccccga cacctatacc ttcggctcga
     1021 acgcgacgcc ggaccaggtc gttcagctga tgctcgagga cttccagacc cggttcgatg
     1081 cgagcatgct gaaggagcag aagagctccg gtctcaccat gcttcaggtg gtcacgatcg
     1141 cttcgatcgt cgagcgcgag gcggcggtgc cggaggagcg tccgatcatc gcgaaggtgt
     1201 acatcaaccg cgtcaacgac ggcatgcagc tgaatgcgga tccgacgctc tcctacatgg
     1261 ccggcaaaga aggggattgg tggccgaaaa tcaccgatgc catcctcgcg aaggagagcc
     1321 cgtacaacac atacaaggtc gtcggtctcc cgccgggtcc gatcgccagc ccgagtctcg
     1381 cctcgctcca ggcggtgctc cagcccgcgg atgtgaccta tctctacttc gtcgcgaaga
     1441 acgacgggag ccagacgcac gcattcgcgg ataccctaga cgagcagaac gccaatacct
     1501 gcacgtacca ggggaccggt tgcgatggat cctccggtgg tgacggtacc gggagcagtg
     1561 gcgacacgcc gacggcaacg gttccgcccg acgacggcaa ctgagatcag gcgcggcgga
     1621 tgattcaggg tggggaaagg tggtcgctta cgtgcttcgg gcgggactga tcggcaatcc
     1681 ggtggcgcac tcgcgatcgc ctcggatgca caatgcggcg ttcgccgcgc tcggcgtcga
     1741 cgctcactac gagctgtggt tgaccgaggt cgaggagatt ccggatcgca tcgccacggt
     1801 gcggggcgca ggaatgctcg gcgcgaatgt gaccgttccc cacaagcagg cggtgatgcc
     1861 tttctgcgac cgcctctccg agacggcaca gcgcgcggga gcggtcaata cgcttatccc
     1921 gaccgccgac ggattgcttg gcgagaacac cgatgtccat ggcttcgcgg aatctgttcg
     1981 ggaggcgatc ggtcgcgatg gcgacgtgcc tccctgcgcg ctcctgctcg gcgcaggtgg
     2041 cgcggcgcga ggtgtcgtgg tcgcattgca acagcttggc gtcggccaca tcacgattgc
     2101 gaaccggaca ctggcgcgcg cccaggagat tgcggacgcc ctcgcaggcg acatcgggac
     2161 gatcgatgcg attccgctcg atcgactggg agacgcggcg cccggggtcg ggttcctgat
     2221 caactccacc tcggtcggat ggcacggcga tgagcttccg gttccggaat ccttcatcga
     2281 ggcattgccg gcctcgacgc tggtggccaa cctgacctat cggcagactg cgctgctccg
     2341 catcgctggg cagcgc
//
LOCUS       DASTMD010000487         3922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2636402, whole genome shotgun sequence.
ACCESSION   DASTMD010000487 DASTMD010000000
VERSION     DASTMD010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2636402"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1546)
                     /locus_tag="VFQ54_07095"
     CDS             complement(<1..1546)
                     /locus_tag="VFQ54_07095"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEU0164796.1"
                     /translation="MDWFNLTLRWYLLTTASTIALAPIALWLCSGLVDRGASVARPIA
                     ALVMIWPIWYLAGIGSGIVPFSVAPLWVALVVIGAASWAFAWRIKLVDRITIRHLLIA
                     EIGFLLFFALYIWFHGYGPQITDQEKPGDMMMLASSMRSTQMPPNDAWMAGDTINYYY
                     LGYVIWAAVAKMVGATPWEAFNLTLATTFGITCVGAAGLAANIAGRWLKENVARLVGL
                     LGLLFVVFMGNPWAAFKSLDHWSSLWKLDFFSGIGWPASRVIHDVITTPPDTIISEFP
                     AFSFILADLHPHLLTLPFAIVSLSLGWALLQGFKAGSLYTVEAMGRVVLTGGIIGALY
                     AMNSWDMPTYLVLAAIALVISTVGFAARERVVSVVVLAVSAVIAWLPFYINFTAPTKP
                     GQTGLARTMGDIPVVGGVLASIVSYHGSRTSAIEYLDVFGFVWVIAVVLIAAEFWNRR
                     HAHVDPLNSRLALGAAAILILGALLIPMPVLALAGLPVVATVVLIQRDPRVTIANVAL
                     GLFSFGF"
     gene            complement(1671..2306)
                     /locus_tag="VFQ54_07100"
     CDS             complement(1671..2306)
                     /locus_tag="VFQ54_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0164797.1"
                     /translation="MISVMLVDDHPVVIEGLRKLLDAAGDIDVTSTARDASTAITQAR
                     TYKPDVILLDLRMSGATGIQATRRLREQEYGGAIIILTSYGDQAYVRQALEAGADGYL
                     LKSTPSDELISSIRNASRGRRQLSPELLSGVLAEFGDLAREKTTRDADLSADDRTILR
                     RASEGATNREIGISMNKSEVAIKKRLQIIFAKLGAVDRAHAVAEAIRRGLI"
     gene            complement(2303..>3922)
                     /locus_tag="VFQ54_07105"
     CDS             complement(2303..>3922)
                     /locus_tag="VFQ54_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0164798.1"
                     /translation="DAIQDQIALTIESGQPAVSKPIQDTEDHAVMLITVPVTTLSDGS
                     MNTSSDDSSTPTPTPVAPGATSTSAQQAATGTNVGAIVAVVNLNYLESLVVPYARGRT
                     EIAVASKDGVIVSTAGIQKNIPQFLQSENDQIEQALEGDSGNFTTTDAGGRQRLAVFS
                     PIQSDVTHWAVIVTSPTPRASAQTLIYQSVVVLCLAGLVILALAVVFGEFTARPLRTL
                     AQRAASMQKGDFSVNIQPVGGGEVRALSTALADMGDQLAAQVQGLEASHQDRDRQANQ
                     MRDLLRRTLRLQEDERRRIAGEIHDAVSPLITGALYQARALQMTNGHTEPEAREESLE
                     SVNKLLEQASEELHGVIFDLRPPDLDDIGVVAAIEAFIQTMQRTGLNCRLEVAAEPSL
                     TPEVRLGIYRIVQEALHNVVRHASADEAVVRLESVGDGLRVTVRDNGTGFDPATSVRP
                     TSLGLLSMRERAAAIGASFTIVSKPGGGTAIVIERADTDSVMSDRVLADLMGETAANE
                     DTGDDMLEDEFQDDAQVTEPASRTQQESKAS"
BASE COUNT          682 a         1199 c         1367 g          674 t
ORIGIN      
        1 gaaagccgaa ggagaagagg ccgagtgcca cattggcgat cgtcacgcgt ggatcgcgtt
       61 ggatgaggac aaccgtcgcg acgactggca agcccgcgag ggcgagcact ggcatcggga
      121 tgaggagcgc gccgaggatc agaatcgccg ccgcgccgag tgcgagcctg gaattgagcg
      181 gatcgacatg ggcatgtcga cgattccaga actcggccgc gatcagcacg acagcgatca
      241 cccagacgaa gccaaagacg tccagatatt cgatcgccga cgtccggctg ccgtggtagc
      301 tcacgatcga ggcgagcacg ccaccgacga cgggaatgtc acccatcgtc ctggcgagtc
      361 cggtttgtcc cggcttcgtg ggcgccgtga agttgatgta gaagggaagc catgcgatga
      421 cggcgctgac ggcgagcacg acaacggaga cgacgcgttc gcgtgccgcg aatccgacgg
      481 tggaaatgac cagggcgatc gcggcgagga cgaggtaggt cggcatgtcc cacgagttca
      541 tggcgtagag cgcgccgatg atgccacccg tgagaacgac ccgccccatc gcttcaacgg
      601 tgtataggct gcccgccttg aatccctgga gcagtgccca cccgagcgaa agcgagacga
      661 tcgcaaaggg aagcgtcagc aggtgtggat ggagatcggc gaggatgaag ctgaaggccg
      721 ggaactcgct gatgatcgta tcgggcgggg ttgtgatgac atcgtgaata acgcgacttg
      781 ctggccagcc gatcccgctg aagaaatcga gcttccagag agaagaccag tggtcgagtg
      841 acttgaaggc ggcccaagga ttgcccatga agacgacgaa gagcagcccg agcagaccga
      901 cgagccgggc gacgttttcc ttcagccagc ggcccgcgat gttcgcggcg agcccggccg
      961 cgccgacgca ggtgatgccg aaggtcgtcg ccagtgtgag gttgaacgcc tcccagggcg
     1021 tcgcaccgac cattttcgcg acggccgccc agatcacata gccgaggtag tagtagttga
     1081 tcgtgtcgcc ggccatccag gcgtcgttcg gcggcatctg cgtcgaacgc atcgaggaag
     1141 cgagcatcat catgtcgccc ggcttctcct ggtccgtgat ctgcggcccg tatccgtgga
     1201 accagatata gagtgcgaag aacagcagga agccgatttc cgcgatcaac aggtgacgga
     1261 tcgtgatccg gtcgacgagc ttgatgcgcc aggcgaacgc ccaggacgcc gcgccgatga
     1321 cgacgagcgc gacccagagc ggggcgacgg agaaggggac gatgccggag ccgattcccg
     1381 ccaggtacca gatcggccag atcatcacca gcgcggcgat cggtcgcgcc acgctcgccc
     1441 cacggtcgac gagaccgctg cagagccaga gggcgatcgg ggcgagcgcg atggtggagg
     1501 cggtggtcag caggtaccag cgaagggtga gattgaacca atccacgcgg cgtcatcctt
     1561 ggcagaaatg cgggcgatgg tgcgtcagtc gctctcgacg tcccgccgaa gtgtaccgtc
     1621 tcgatctcgt tcgcggattt ccggcggcaa ccgtgatccg ccagcgccgc tcagatcagc
     1681 ccgcggcgga tcgcctcggc gacggcatgc gcgcggtcga ctgcgcccag cttcgcgaag
     1741 atgatctgca gccgcttctt gatcgcgacc tcgctcttgt tcatcgagat cccgatctcg
     1801 cggttggtcg cgccttcgct cgcgcggcgg aggatcgtgc ggtcgtcggc cgagagatcg
     1861 gcgtcgcggg tggtcttctc gcgtgcgaga tcgccgaact cggcgagcac gccgctcagg
     1921 agttccgggc tgagctggcg acggccgcgc gaggcgttcc ggatcgagga gatgagttcg
     1981 tcggacggcg tgctcttcag gaggtagccg tcggcgccgg cctcgagtgc ctgccgcacg
     2041 tatgcctggt ccccgtatga ggtgaggatg atgatcgccc cgccgtattc ctgctcccgg
     2101 aggcggcggg ttgcctggat tccggttgcg ccggacatcc ggagatcgag caggatcacg
     2161 tccggcttgt atgtcctcgc ctgcgtgatt gccgtcgatg cgtcacgcgc cgtgctcgtg
     2221 acgtcgatgt cgccggcggc gtcgagcagt ttgcggagtc cctcgatcac cacggggtga
     2281 tcgtcaacca gcatgaccga tatcatgacg ccttgctttc ctgttgcgtt cgcgatgcgg
     2341 gttccgtcac ctgggcgtcg tcctggaatt cgtcttccag catgtcgtcg ccagtgtcct
     2401 cattggcagc ggtttctccc atcaggtcgg cgaggacgcg gtcggacatc acgctgtcgg
     2461 tgtccgcccg ctcgatgacg atcgccgtgc cgccacccgg cttgctgaca atggtaaatg
     2521 acgcgccaat cgccgccgcg cgttcccgca tcgagagaag cccgagcgat gtcgggcgaa
     2581 cggaggtcgc gggatcgaat cctgtgccat tgtcgcgcac ggtgacgcga aggccgtcgc
     2641 cgaccgactc cagccggacg acggcttcgt ccgcgctggc gtgccggacg acgttgtgca
     2701 gggcttcctg gacgatgcga tagatgccga gacggacttc cggggtcagg gacggttccg
     2761 ccgccacctc gaggcggcag ttcagacccg tgcgctgcat cgtctggatg aaggcttcga
     2821 tcgcggcgac gacgccgatg tcgtcgagat cgggcgggcg gagatcgaag atgacgccgt
     2881 gcagttcttc ggatgcctgt tcgaggagct tgttgacgct ctccagcgac tcctccctcg
     2941 cctccggctc cgtgtggcca ttcgtcatct ggagcgcgcg cgcctgatag agcgcaccgg
     3001 tgatgagcgg ggaaacagca tcgtgtatct cgccggcgat gcgacggcgc tcgtcttcct
     3061 gcagccgcag ggtgcggcgc aggagatcgc gcatctggtt cgcctgccgg tcgcgatcct
     3121 gatgactcgc ctcgagcccc tgcacctgcg ccgcgagttg gtcgcccatg tcggcgagcg
     3181 cagtgctgag ggctcggacc tcgccgccgc cgaccggctg gatgttgacg gagaagtcgc
     3241 ccttctgcat cgaggcggca cgctgggcaa gcgtgcgaag cggtcgcgcc gtgaactcgc
     3301 cgaagacgac ggcgagcgcg aggatgacga gaccggcaag gcagagcacg accacgctct
     3361 ggtagatcag cgtctgcgcg gacgcgcgcg gggtcggact ggtgacgatg acggcccagt
     3421 gggtgacgtc ggactggatc ggcgagaaga ccgccaggcg ctgacgtccg ccggcgtccg
     3481 tggtcgtgaa gttcccggag tcgccttcga gtgcctgttc gatctggtcg ttttccgact
     3541 ggaggaactg cgggatgttc ttctggatgc ccgccgtgga gacgatcacg ccgtccttgc
     3601 tggcgacggc gatttcagtg cgaccacgcg cgtagggaac caccagcgac tcgaggtaat
     3661 tcaggttcac gacggcgacg atcgcgccga cgttggtccc ggtcgccgcc tgctgggcgg
     3721 acgtactcgt cgcgcccggg gccacgggcg ttggtgtcgg ggtcgacgag tcatcgctcg
     3781 acgtattcat cgaaccgtcg gagagcgtgg tgaccggcac cgtaatcagc atcacggcgt
     3841 gatcctcggt gtcctggatc ggcttcgaga cggccggctg gccactctcg atggtgaggg
     3901 cgatctggtc ctggatcgcg tc
//
LOCUS       DASTMD010000488         4582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1117860, whole genome shotgun sequence.
ACCESSION   DASTMD010000488 DASTMD010000000
VERSION     DASTMD010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117860"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(397..2010)
                     /locus_tag="VFQ54_07110"
     CDS             complement(397..2010)
                     /locus_tag="VFQ54_07110"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEU0164799.1"
                     /translation="MAHDEILPVIRFDRRRMLGIASGTVAATMFGPLGLAGAADVSAN
                     VLAAYTQSAGFGDLVPDPKGRLALPEGFSYTEISPAGSKLTNGDLVPDWHDGSAAFPG
                     ADGNIILIRNHEITYEPKEDPSILRVPASKPYDSEAMGGTTAVVIAPDRTVVESFVAS
                     SGTLSNCAGGATPWGTWLTCEEVTSKILLDEDEITTKLPHGYVYEVDPSDPEGELSRT
                     PIKDMGTFSHEAVGFDTETGIIYLTEDGDTDASDTDPSKDTGGSFLYRFTPTDKSQKP
                     GALQKGGKLESLKAVKAPTTNDADLLDQGNTLSVTWVEVDKDDTTADALAKGCLHFNR
                     LEGASFAAGTLWFCDTNGGEQRLGQIFRYTPKTNMLELFYEGTDGAGYPGSDAGTDFA
                     RLESPDNITVAPWGDIIVAEDGGGVNRLIGFTPDAESYVMALHNLPYPTLEEEGLPVN
                     FQSEVTGPTFSPDGKTLFFNVQYPGTTFAVWGPFKEPSAERQALMAVAPAPAALAPHV
                     SPELRAAASRHNLTIGQALAFHRLGVLSI"
     gene            2788..3294
                     /locus_tag="VFQ54_07115"
     CDS             2788..3294
                     /locus_tag="VFQ54_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0164800.1"
                     /translation="MDRYDSRRRQRLPGYDYAQANVYFVTITTHQFVARFGTIRNATV
                     ELNDAGRTVERFWRLIPRRYPTAIIDAFVVMPDHFHGILVLGGESKGRPTLPRIMQWF
                     KSWTTTEYATGVQEMDWEPFDGKLWHTGYYAHICRDEIDIERIRAYIESNPDRRWRKM
                     LLGKEAPS"
     gene            3494..4348
                     /locus_tag="VFQ54_07120"
     CDS             3494..4348
                     /locus_tag="VFQ54_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0164801.1"
                     /translation="MKLGVSSYSFATAFATGTMDILDAIDWIAGSSASHLEISLAGLG
                     QDLIEQPDLVAAIKQRATEKNVPLVNYVVGANFRDSDLAGQIARLKKHIDIAHDLGIT
                     HFRHDVVEWGWRDPSQKEFEQVFATVVPVTQEIADYAAPLGIVTSVENHGFGFNNSER
                     VRRLVDAVDRPNFRTTLDIGNFLCVDEQPIYAVAMNLPYASVIHLKDFYVRDERPGDG
                     WLKTVAGNHIRGAIVGYGDLPMRRLLTLIKESGFDGPISIEFEGIEDDLLAIQTGLAN
                     AVRIWDEI"
BASE COUNT          869 a         1482 c         1405 g          826 t
ORIGIN      
        1 acccggaacg gcgacaagcg ccgtggggac gggaatggca gcgccttgct cgaaagtcat
       61 cgcgaacggg gagaccatga cgatcaccag gatcgcccct acgacatcgt ccacaaatga
      121 cgcctttccg ctcgacaacc ctgtccgtca cgactgggac gcgaccacat gggtcgcagg
      181 gacgggatgg gagcgcgtcg ttccgacgac acgttgtggt cgaggattgc gacatcccgt
      241 acggcgggcg ccaaggacgc cttccataag aagacagaac gcgaggaggc ccgtcgtcaa
      301 tgacgggcct cctccctcga tctgaattcg cggtcgtgct ggatccggcc ccggggtcga
      361 cgcaacaggt cgatcgtctc ccggaatccc tctctgctag atcgagagca cacccagccg
      421 gtggaaggcg agcgcctgtc cgatcgtcag gttgtggcgg gacgccgcag cgcggagttc
      481 aggagagacg tgcggcgcca gcgcggccgg ggccggagcg accgccatca gcgcctgtcg
      541 ctcggcggac ggctccttga acggtcccca gaccgcgaag gtcgtgcccg ggtactggac
      601 gttgaagaag agcgtcttgc cgtccggcga gaaagtcggg ccggtgacct cggactggaa
      661 gttgaccggc aagccctcct cctcgagcgt cggatacggc aggttgtgca acgccatcac
      721 gtagctctcg gcatccggcg tgaagccgat cagccggttc acgccgccac cgtcctccgc
      781 gacgatgatg tcaccccacg gcgcgacggt gatgttgtcc gggctctcga ggcgggcgaa
      841 atcggtgccc gcgtccgacc ccggataccc ggcgccatcg gttccttcat agaagagttc
      901 cagcatgttg gtcttcggcg tgtagcggaa gatctggccg agccgctgct cgccaccatt
      961 ggtatcgcag aaccagagcg tgcccgccgc gaaggaggcg ccctcgaggc ggttgaagtg
     1021 caggcagccc ttggcgagcg catcggcggt cgtatcgtcc ttgtcgacct cgacccaggt
     1081 gacgctcaac gtgttgccct gatcgagcag gtcggcatcg ttcgtcgtcg gcgccttcac
     1141 ggccttgagg gattcaagct tgccaccctt ctgcagggcg cctggcttct ggctcttgtc
     1201 ggtcggggtg aagcgataga ggaagctgcc acccgtgtcc ttgctcggat cggtatcgct
     1261 ggcatccgta tcgccgtcct cggtgaggta gatgatgccg gtctcggtgt cgaagccgac
     1321 ggcctcgtgc gagaaggtgc ccatgtcctt gatcggcgtg cgggaaagct cgccttcagg
     1381 atcggacgga tcgacttcgt agacgtagcc gtgcgggagc tttgtcgtga tctcgtcctc
     1441 gtcgaggagg atcttcgagg tcacttcctc gcaggtgagc caggtgcccc acggggtcgc
     1501 gccaccggca cagttggaaa gcgtgccgga ggaggcgacg aagctctcga cgaccgtgcg
     1561 atccggcgcg atcacgacgg cggtcgtgcc gcccatcgcc tcactgtcat aaggcttgct
     1621 cgccggaacc cgcaggatgc tgggatcttc cttcggttcg tacgtgatct cgtgattgcg
     1681 gatcaggatg atgttgccgt cggcgccggg gaaggcggcg ctgccatcgt gccagtccgg
     1741 gacgaggtcg ccattggtca gcttgctgcc tgccggcgag atctcggtgt aggagaagcc
     1801 ctccggcaac gcgaggcgtc ccttcggatc cggcacgagg tcgccgaaac cagccgattg
     1861 cgtataggcg gcgagcacgt tcgcgctcac gtcggccgcg ccggccaggc cgagcggacc
     1921 aaacatcgtg gccgcgacgg taccactcgc gatgccaagc atgcggcggc ggtcgaaccg
     1981 gatcactgga agaatctcgt catgcgccac gttgtctctc ccatacattc accagcggca
     2041 agatcgccac aaacgcaggg gcatgccgcg aaacgccctt gtttcactgc atgccccgtg
     2101 ttcatccagc ctatttggtg agacgtagta tccaatcaac gcaatgataa ttcttctcag
     2161 ctgatacctt ttcgtaaaga ccatcgcgat gctgttgatg acccatcctc atatcaggac
     2221 atctcggcgt cacgcgcaac acggtcgcgc gagatgtcgc actcggggcc atcccgttgc
     2281 gcatccacgc gatccctcac ctcccgagta gtcatgaagc gctcgcctcg ccagcgcggc
     2341 atgaaaaagc ttgggtcgga gggcccgaag ggcggagttt ttcagagcag cttgtcgagg
     2401 aatcgcgtcg gcaccgcgtg ccgtgcgtac aacccgtccc ccagacacgt cctcccgagc
     2461 tcgtcgaggg accatgtcgg caccacgtgc catgcgtgcg acacctcccc cggaccccaa
     2521 cggggactcg cgcctttgcc atgcatgcgt tcagaaacgg atcggctgcg ccgcatcgac
     2581 ccgaaacggc gacaagcgcc gtggggacgg gaacgccagc gcgccgttcc ggcggcatgg
     2641 ggtacgggaa aacgacggcg actcgccgga gcgagaggcg ggacacggag gtcccgccgt
     2701 acgaacgacc gctgcctcaa cgattctcta tgcgagaatc agcctcgggt cgcggacttc
     2761 atccaaaacg ggtggagtga agaattcgtg gatcgatatg actcacgccg acgccaacga
     2821 cttcccggct acgactacgc tcaggcaaac gtctattttg taacgatcac aacccatcaa
     2881 ttcgtggcca gattcggcac gatccgcaac gccaccgtcg agctgaacga cgccgggcgc
     2941 acggtcgaac gcttctggcg cctgatcccg agacgatacc ccacagcaat catcgatgcg
     3001 ttcgtcgtca tgcccgacca tttccacggg attcttgttc tcggaggtga gtcgaaggga
     3061 cggccaacac ttcctcgaat catgcaatgg ttcaaatcct ggacgacaac cgaatatgcg
     3121 acgggtgttc aggaaatgga ttgggagcca ttcgacggca aactctggca cacggggtat
     3181 tacgctcata tctgtcgcga cgaaatcgat atcgagcgga ttcgcgcata catcgaaagc
     3241 aaccccgacc gccgttggag aaagatgctg ttggggaagg aagcccccag ctgacagccg
     3301 cctgccgcga cgcggtcgtt cgtacggcgg gaccaccgtg tcccgccttt tgtcctgggc
     3361 ggcttggggc catcatctcc ctgaccagga cctggtttgc atcttcatcc ggttccgcat
     3421 accgtgtccg aattgacgac gaaccgatcg gttcgttcga tgagcatgca gcaccacgag
     3481 gagtttccgc acgatgaaac ttggcgtcag ttcgtacagc ttcgccaccg ccttcgcgac
     3541 gggcacgatg gatattctcg acgccatcga ctggatcgcg ggttcctccg ccagccacct
     3601 cgaaatctcg ctcgccggac tcggtcagga tctgatcgag cagccggacc tcgtcgccgc
     3661 catcaagcag cgcgccaccg agaagaacgt gccgctggtg aactacgtcg tcggtgcgaa
     3721 cttccgggac tccgatctcg ccggccagat cgccaggctg aagaagcata tcgacatcgc
     3781 ccacgacctc ggcatcaccc acttccgcca cgacgtcgtc gaatggggct ggcgcgatcc
     3841 gagccagaag gaattcgagc aagtcttcgc gacggtcgtc ccggttaccc aggagatcgc
     3901 cgattatgcc gcgccgctcg ggatcgtcac cagcgtcgag aatcacggct tcggcttcaa
     3961 taacagcgag cgcgtccgcc gcctcgtcga tgcggtcgac cgtcccaact tccgcacgac
     4021 gctcgatatc ggcaacttcc tctgcgtcga cgagcagccg atctacgcgg tcgcgatgaa
     4081 tctcccgtac gcttccgtca tccatctcaa ggacttctac gttcgggacg agcgtcccgg
     4141 cgatggctgg ctgaagacgg tcgccggcaa ccatatccgt ggcgccatcg tcggctacgg
     4201 cgacctgccg atgcgccggc tcctcacgct gatcaaggaa tccggcttcg acgggccgat
     4261 ctcgatcgaa ttcgaaggca tcgaggacga tcttctcgcg atccagaccg gcctcgccaa
     4321 tgccgtccgg atctgggacg agatctaggg cgcaatccgg cttcagcccc aacccgtggt
     4381 tcgagcgaag gcggagggag acttctcccc cgccttcctc tggccccacc cctcgcaatc
     4441 gggaagaagc gagaccaccg gaacctgcct atcctgaatc aaagggcgat cgacggtgtc
     4501 gtcgcccgcc cttctcatcg tgtggcaacc agacgccacg aagggagatt cggcacatgg
     4561 ctacctacgt tctgattcac gg
//
LOCUS       DASTMD010000489         5835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_670784, whole genome shotgun sequence.
ACCESSION   DASTMD010000489 DASTMD010000000
VERSION     DASTMD010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670784"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VFQ54_07125"
     CDS             complement(<1..675)
                     /locus_tag="VFQ54_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0164802.1"
                     /translation="MQSTGSTVEVKYWTAFPSGVNGDAQKKLVDDFHAAQSDVKINMQ
                     TQASYEDLAAALTAALQTGDEPHLVVLSDVWWFRFYLAEALADLNPLLKAANIDTADY
                     VQSLYTEYTRNGGQYAAPFARSTPLLYYNQDALDAANVDASSLATWDGIAEVAPKLTG
                     GKTKYAFGFGNAASYGAWVLQGAVWAFGGHYSTPDFTITLADAPAVATGEFMRKLVSD
                     GYGISTE"
     gene            complement(1055..1927)
                     /locus_tag="VFQ54_07130"
     CDS             complement(1055..1927)
                     /locus_tag="VFQ54_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0164803.1"
                     /translation="MINLSPKQQETTGRVAGYIGLAILVLIIGIPMFWMLSSSLKTYR
                     EVYTFPPQWIPSNPRWSNYKDAWDAAPFGRFYLNTVIITVCGTGLQLFNGILCAYALA
                     FLKFPFKSGFMVLILAALMIPGEVTILPNYLFMGNTVRDWFGLSDNWVNTYQGIILPG
                     AATAFGTFLMRQGFKGLPREVLEAAKLDGAGHLRTMWDIVLPMARPMVVTFAVISVVN
                     KWNDYLWPLIITNTDQMRPLTVGLTFLFDNEGNNQWGIIMAGTAFVIVPLIVVFLFAQ
                     RFIIEGLTAGATKG"
     gene            complement(1920..2888)
                     /locus_tag="VFQ54_07135"
     CDS             complement(1920..2888)
                     /locus_tag="VFQ54_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0164804.1"
                     /translation="MTETVVQRRDATGAIGASIPIEQARPRRRKLANQEWVIFILFMF
                     PNLFFLSLFTFWPMIKNVEISFHRSNLLNQHSTWVGLDNYRYIFEDAQFQQVLRNTLV
                     FTISTLVATLVLGLLIALLLNQKLRFRNAARAIVFAPTLLSGAAIGIVWAYIFDPRYG
                     LLAQMLGWIHITSPQWLNRPEWAMPAIIIVYVWKNVGFSAVIFLAGLQAIPKDLYDAA
                     KVDGANVFWRFRSVTLPMLSPVSFFLLITTVLATFQAFDIQRVMTRGGPAFSTTTLIY
                     YVYEQGFGLAANYGRAAAAAITLFFVMLVITVIQFRFSENQVHYDD"
     gene            3094..3801
                     /gene="tsaB"
                     /locus_tag="VFQ54_07140"
     CDS             3094..3801
                     /gene="tsaB"
                     /locus_tag="VFQ54_07140"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU0164805.1"
                     /translation="MTDFTFPVLLALDTSTDQATVGLTNGDLFAELRWPAGRSQTTSV
                     LPAIDHLLTMNGLTMNAIAAIAVAIGPGTFTGLRVGLSIAKGLSLAKDIPLVSIKTLA
                     ITAYPYSGLGRPLLVTLPAGRGRVVWEIHDNGGVSEPHNTTVPELIEVLVGRPDLFVA
                     GELLPEHRATIAESHSLIESVGVAGRRGSVLAQLGWERWRRGDVDDAATLEPIYVHGR
                     PASTAPIEDRLRRKSTR"
     gene            3967..4926
                     /locus_tag="VFQ54_07145"
     CDS             3967..4926
                     /locus_tag="VFQ54_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0164806.1"
                     /translation="MNAITSASNGVVATAVDAFPTVDALEQALRDQSYVTDRGLATTL
                     YLTLKLQRPLLLEGEAGVGKTEIAKVLSAALNTPLIRLQCYEGLDASSAIYEWDYPRQ
                     MLYLRSLEATGTLNQDEVRKNLFGEDFLLKRPLLQAIDVAHDRAPILLIDEVDRADQE
                     LEAFLLEILSDFQVTVPELGTLRAEHVPIVILTSNRTREIHDALKRRCLYHWIDFPTY
                     EKEIQILNAKVPEVSGQLAKEICAFTQGLREIDLFKSPGMAETLDWANALLALGSNQL
                     QDQVVEDTLGVILKYQEDVDRVRGDVTQRLVQRALTSAAANRG"
     gene            4931..>5835
                     /locus_tag="VFQ54_07150"
     CDS             4931..>5835
                     /locus_tag="VFQ54_07150"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0164807.1"
                     /translation="MSELRPADMLDNSRVRAERVASNLLLFGRRLRSGGMPVGPGQVI
                     GLVEALGAIDINRRDDVYSAAKASLINRPEQIPVFDAEFKRFWRDLVQQDAPLLTEYA
                     ADDDESVGVPDTKNTGEQSEQGQSGQGGEADRSVLAIEGLDQAGDIGETEDYEVPPED
                     VLIFSASEALRKKDFAQFTAEEIAEARRIIEGMTWRLGTRKTRRRERALHGEFIDHRA
                     TLRSSMKQGGVPLDLKWRTKKERMRPLVLICDISGSMDRYARLLLRFVHALEHGLDSV
                     EVFVFGTRLTRITRELRRRDVDAAIL"
BASE COUNT         1107 a         1749 c         1900 g         1079 t
ORIGIN      
        1 ctcggtgctg ataccgtacc cgtccgagac gagcttgcgc atgaactcac cggttgcgac
       61 ggccggagca tcagcgagcg tgatggtgaa gtccggggtc gagtagtgac cgccgaaggc
      121 ccagaccgca ccctggagga cccaggcgcc ataggaggcg gcgttgccga agccgaacgc
      181 gtacttcgtc ttgccgccgg tcagcttcgg cgcgacctcg gcgatgccgt cccaggtggc
      241 gagcgacgag gcgtccacgt tcgccgcatc gagggcatcc tggttgtagt agaggagcgg
      301 cgtcgagcgg gcgaacggcg cggcgtactg gccgccgtta cgcgtgtact cggtgtagag
      361 ggactgcacg taatcggcgg tatcgatgtt cgccgccttg agcagcgggt tgagatcggc
      421 gagcgcctcg gcgaggtaga agcggaacca ccagacgtcg gacagcacca cgagatgcgg
      481 ctcgtcaccg gtctggaggg cggccgtcag ggcagcggcg aggtcctcgt aggaggcctg
      541 cgtctgcatg ttgatcttga cgtccgactg cgcggcgtgg aaatcgtcca cgagcttctt
      601 ctgcgcatcg ccgttgacgc cgctcgggaa tgccgtccag tacttgacct caacggtcga
      661 gccggtggac tgcaccaggg tcggggcggc aaagacgctg ctcggcttga tgccgagtgt
      721 ggcggcggag gtcgcgaggg cagcgccgac ggcggcaccc ttgaccagag ttcgtcgcta
      781 caggatttcc ttcattactt cgatcctcct cgatcgctcg ttcgttccca cccgcatctg
      841 ccacccggca gaccggtgag tgagttggtt gcaagacctg ggacaccgtg tcccagctca
      901 ttgttcaacc aatgcgacgc tgcattgttg aactcgtgct aacttccgga gaaaaaaccg
      961 gaatcagaac cgattggtga aggctttggc caatcccccg gggtatatgt aaagaacgac
     1021 atatgaatcg tcgcctttcc gcaaaatgtg cggcctaccc cttggtcgcg cccgccgtga
     1081 gtccctcgat gatgaaccgc tgtgcgaaga ggaagacgac gatcagcggc acgatcacga
     1141 atgcggtgcc ggccatgatg attccccact ggttgttccc ctcgttgtcg aagaggaacg
     1201 tgaggccgac cgtcagcgga cgcatctggt cggtgttcgt gatgatcagc ggccagaggt
     1261 agtcgttcca cttgttgacc accgagatga cggcgaaggt gacgaccatc ggtcgcgcca
     1321 tcggcaggac gatgtcccac atcgtgcgca ggtggccggc gccgtcgagc ttcgccgcct
     1381 cgagcacctc gcgcggcaac cccttgaacc cctgtcgcat caggaacgtg ccgaacgcgg
     1441 tggcggcgcc gggaaggatg atgccctggt aggtgttgac ccagttgtcg gagagtccga
     1501 accagtcacg caccgtgttg cccatgaaga ggtagttcgg gaggatcgtg acttcgccgg
     1561 ggatcatgag ggcggcaagg atcagaacca tgaagccgct cttgaacggg aacttcagga
     1621 acgccagcgc gtatgcgcag aggatgccgt tgaagagctg caaccccgtc ccgcagacgg
     1681 tgatgatcac cgtgttcagg tagaagcgac cgaacggcgc ggcgtcccag gcgtccttgt
     1741 agttggacca gcgcgggttg ctcgggatcc actgcggcgg gaaggtgtag acctcgcgat
     1801 aggtcttcag cgagctggag agcatccaga acatcgggat gccgatgatg agcacgagta
     1861 tcgcgaggcc gatgtacccg gcgacccgtc ccgtcgtctc ttgttgcttc gggctgaggt
     1921 taatcatcgt agtgcacctg attctcggag aagcggaact ggatgacggt gatcacgagc
     1981 atcacgaaga agagcgtgat cgctgccgcg gccgcgcgac cgtagttcgc ggcgagaccg
     2041 aagccttgct catacacgta atagatgagc gtggtcgtcg agaacgccgg acctccccgg
     2101 gtcatgacgc gctggatgtc gaacgcctgg aatgtggcga gcacggtcgt gatcaggagg
     2161 aagaaggaga ccggtgaaag catcggcagg gtcaccgagc ggaagcgcca gaagacgttc
     2221 gcgccgtcga ccttggcggc gtcatagagg tccttcggga tcgcctggag cccggccagg
     2281 aagatcacgg cggagaagcc gacgttcttc catacataga cgatgatgat cgccggcatc
     2341 gcccattccg ggcggttcag ccactgcggc gacgtgatgt ggatccagcc gagcatctgg
     2401 gcgagcagcc cgtaccgggg atcgaagata taggcccaga cgatgccgat cgcggcgccg
     2461 gaaaggagcg tcggcgcgaa gacgatcgcg cgggcggcgt tccggaagcg gagcttctgg
     2521 ttgagcagca gtgcgatcag gagtccgagg acgagcgtcg cgaccagggt cgagatcgtg
     2581 aagaccagcg tgttccggag gacctgctgg aattgcgcgt cctcgaagat gtaccggtag
     2641 ttgtccagtc cgacccacgt actgtgctga ttgagcagat tcgagcggtg gaaactgatc
     2701 tcgacgttct tgatcatcgg ccagaacgtg aagagcgaca ggaagaagag attcggaaac
     2761 atgaagagga tgaagatcac ccactcctga ttcgccagct tgcgacggcg gggcctcgcc
     2821 tgctcgatgg gaatcgatgc tccgattgcg ccggtggcat ccctgcgctg gacgaccgtc
     2881 tcagtcattc gcgttcctcg gtttcgtctc gttcgatcgt tccgtcgcca ttggaacgca
     2941 ccacgatgac cccaatacaa ttgccgttcg gacgggcgca ctcgcgcaat ccccgacgaa
     3001 aaggaagtct gcaacatgat tcgcgccaga tggcttgagg attgttaaca ttttcaaatt
     3061 gcagctgaat ctattgggta cgaggcgaat ttgatgaccg atttcacgtt tccggtgctg
     3121 ctcgcactcg acacttctac cgatcaggca actgttggcc tgacgaatgg cgacctgttt
     3181 gcggaattgc gctggccggc ggggcgatcg cagacgacga gcgtcctccc ggcgatcgat
     3241 catctgctga cgatgaatgg tctgacgatg aacgccatcg cggcgatcgc cgtggcgatc
     3301 ggtcctggca cgtttacagg gttgcgcgtg ggattgagca tagcgaaggg gttgtcgctt
     3361 gccaaggata ttccgctggt aagtattaag acgttagcaa ttacagcgta tccctattcc
     3421 ggactgggac gacccctgct cgtgacgctg ccggcgggcc gcggacgcgt cgtctgggaa
     3481 attcatgata acggcggggt ttccgagccc cacaacacga cggtgcccga gctgatcgag
     3541 gttctcgtgg ggcgcccgga cctcttcgtg gccggagaat tgttgccgga acaccgtgcc
     3601 acgatcgccg aatcccatag cctgatcgag tcagtcgggg ttgcgggacg acgcggatcc
     3661 gtgctcgccc agctcggttg ggagcgctgg cggcgtggcg acgtcgacga cgccgcgacg
     3721 ctggagccga tctacgtgca cggccggccg gcgtcgacgg cgccaatcga ggatcggctg
     3781 agacgaaaat ccacccgctg acgtcgattt cttgggccgc gagcccaagt catcgctgtc
     3841 cgccggggcc cgcattgacc ggatatggcc aaggttgcac gcgtcgggga tactaaacag
     3901 atagacagcc ccgatctgtc acccgtttcg ggttgcgccc ctcccattca ctcagcgagg
     3961 tcctctttga acgcaatcac ttctgcttcc aatggcgttg tcgctacggc agtcgacgcc
     4021 tttcctaccg ttgacgctct cgaacaggcg ctccgcgatc agtcctacgt cacggatcgc
     4081 ggtctggcga cgacgctcta tctcacgctg aagctgcagc gaccgctgct cctcgaaggc
     4141 gaggccggcg tcggcaagac cgagatcgcg aaggtcctct ccgcggcgct caataccccc
     4201 ttgattcgcc tgcagtgcta cgagggtctc gacgcctcct cggccatcta tgagtgggat
     4261 taccctcgcc agatgctcta ccttcgctcg ctcgaggcga ccggcaccct gaaccaggac
     4321 gaggttcgca agaacctgtt cggtgaggac ttcctgctca agcgaccgct gctccaggcg
     4381 atcgatgtcg cccacgaccg cgcgccgatc ctgctgatcg acgaggtcga ccgcgccgac
     4441 caggagctcg aggcattcct gctcgagatc ctctccgact tccaggtcac cgtgccggag
     4501 ctggggacgt tgcgtgccga gcacgtgccg atcgtgatcc tcacctcgaa tcgtacccgc
     4561 gagattcacg acgccctgaa gcgacgttgc ctctaccact ggatcgactt cccgacctac
     4621 gagaaggaaa tccagatcct caacgcgaag gtgccggagg tttccgggca actcgccaag
     4681 gaaatctgcg cctttaccca gggcctgcgt gagatcgacc ttttcaagtc gccgggtatg
     4741 gccgagacgc tcgactgggc caacgccctg ctcgcgctcg gcagcaacca gctccaggat
     4801 caggtcgtcg aggatacgct cggcgtcatc ctcaagtacc aggaggatgt cgatcgcgtg
     4861 cgtggcgacg ttacccagcg cctcgtgcag cgcgccctga cgtccgccgc cgcgaatcgg
     4921 ggatagcacc gtgagcgagt tgcgaccagc ggacatgctc gacaactcac gcgttcgggc
     4981 cgaacgcgtg gcgtcgaatc tcttgctctt tgggcgccga ttgcgctccg gcggcatgcc
     5041 ggtcggaccg ggtcaggtga tcggcctggt cgaagcgcta ggtgcaatcg acataaaccg
     5101 gcgcgatgat gtctattcgg cggcgaaggc gtcgctgatc aaccggcccg agcagattcc
     5161 cgtcttcgat gccgagttca agcgcttctg gcgcgatctc gtgcaacagg atgcgccgct
     5221 tctgaccgag tacgccgccg atgacgacga gtctgtcggc gttccggata cgaagaacac
     5281 cggcgagcag tcggagcagg ggcaatcggg tcagggcggc gaggccgatc gctccgtcct
     5341 cgccatcgag ggactcgacc aggcgggcga catcggcgag accgaggatt acgaggttcc
     5401 gcccgaagat gtattgatct tcagtgcgag cgaggccctt cgcaagaagg acttcgccca
     5461 gttcacggcg gaagagatcg ccgaggcgcg acggatcatc gagggcatga cctggcgact
     5521 cgggacgcgc aagacgcggc ggcgcgagcg ggcgcttcat ggtgagttca tcgatcaccg
     5581 tgccacgctt cgcagctcga tgaagcaggg cggcgtcccg ctcgatctga aatggcggac
     5641 caagaaggag cggatgcgtc cgctcgtgct gatctgcgac atctcgggct cgatggatcg
     5701 ctacgcccgg ttgttgctgc gattcgtcca cgcgctggaa catggcctgg attcggtcga
     5761 ggtcttcgtc ttcggcacgc ggttgacccg catcacgcgg gagcttcgcc ggcgtgacgt
     5821 cgatgccgcg atcct
//
LOCUS       DASTMD010000490         1848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1028638, whole genome shotgun sequence.
ACCESSION   DASTMD010000490 DASTMD010000000
VERSION     DASTMD010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028638"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(699..1688)
                     /locus_tag="VFQ54_07155"
     CDS             complement(699..1688)
                     /locus_tag="VFQ54_07155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU0164808.1"
                     /translation="MGSIGYAASFEQFHPTDLLKYSQLAEKNGFSVITASDHFHPWVP
                     SQGHSAFVWSWMGALGATTNLRFGTGVTPPGWRYHPAILAQAAATLEAMYPGRFFLGL
                     GAGEALNEHIVGDYWPEAPTRLDRLVEAIEIIEKLFTGKVVKHSGKHFTVESAKLYTL
                     PDAPPPIYVATSGPIMAARTGKLTEGIITVGAADEKIKMLLDRFDKGARDAGKDPNTL
                     AKILQVKVSYATTEQEAIDSAMKDWPNGGMNFPKADIRNPEDFEAMSKLVRPENYKNR
                     VLTTADLSEHVAYLQHFIDLGFREIHIHNVNRNQEGFIEAYGKDVLPKLNWPS"
BASE COUNT          326 a          592 c          593 g          337 t
ORIGIN      
        1 cccgcagcat agataagcgg ggcagcaccg ctgaggcggt acggatcgtc ccgcctggca
       61 taccgagaac gccgcgagac ccctgcgctc ggcgagtcga tccgggaatg ggctgttcgc
      121 cggcccccgg tggtcggatc ggaaaccagc cgcttgccac cgggaggcag gaggcaggac
      181 atacttccgg cgcacgaccg cccaaccgct cgtgcgcaaa cgatgtgcga tcgtgcgcct
      241 catagccccg ccccacgtac gaacgctggg cggatcgccc tgatccgaga tcaagccgag
      301 cgacacggtg gcacctacag cgaaatcgaa acggttgtca catgccgtcc gagattaaat
      361 gcgatgcctc gttaagcgca cggtcgttcg tagggcggga ccgccgctgc cggaccggag
      421 agcctgccct gagcttgccg aatgggtccg gacggcctgt cccacctcgc gtttgcccac
      481 ggatcgcgct cccggtggcg actattccgg agccttcgac acgttttgtg gtgctggggt
      541 tggtagtgcg gggtttcata acccgccgca acccaaacac acgagaaaca cgccgtgccc
      601 cgaggtagtt accgctacgg gctggccaac cgaatcgtcc gaaacgcgaa gagccccggc
      661 gagtgatcgc cggggctccc atgttgcact tgccaggtct aggatggcca gttcagcttc
      721 ggcaggacgt ccttgccgta ggcctcgatg aacccttcct gattgcgatt cacgttgtgg
      781 atgtgaatct cccggaagcc gagatcgatg aagtgctgca ggtacgcgac gtgctcgctg
      841 aggtccgccg tcgtcagcac ccggttcttg tagttctcgg ggcggacgag cttcgacatc
      901 gcctcgaagt cctccgggtt ccggatgtcc gccttcggga agttcatccc gccgttcggc
      961 caatccttca tcgcggagtc gatcgcctcc tgctcggtgg tcgcgtaact gaccttcacc
     1021 tgcaggatct tcgcgagcgt gttcggatcc ttgccggcgt cacgcgcgcc cttgtcgaag
     1081 cgatcgagaa gcatcttgat cttctcgtcg gccgcgccga cggtgatgat gccctcggtc
     1141 agcttgccgg tgcgggccgc catgatagga cccgacgtcg cgacgtagat cggcggtggc
     1201 gcatccggca gcgtgtagag cttcgcgctc tccaccgtga agtgcttgcc cgaatgcttg
     1261 acgaccttgc ccgtgaagag cttctcgatg atctcgattg cctcgacgag gcggtcgagc
     1321 cgggtcgggg cctccggcca gtagtcgcca acgatatgct cgttcagcgc ctcaccggcg
     1381 ccgagaccga ggaagaaacg acccgggtac atcgcctcga gcgtcgcggc ggcctgggcg
     1441 aggatcgccg gatggtagcg ccagcccgga ggcgtgacgc cggtgccgaa gcgaagattg
     1501 gtggtcgcgc ccagtgcacc catccaggac cagacgaaag cggaatggcc ctgcgacggc
     1561 acccagggat ggaagtggtc cgacgcggtg atgaccgaga agccattctt ctcggcgagc
     1621 tgactgtact tgagcagatc cgtggggtgg aactgctcga acgacgctgc gtatccgatg
     1681 gagcccatga ttcctccgat tgattttcag gcgaggacgt ttctcgcctc gaactcaaac
     1741 gctagttgaa gagattgcgg tcttccgcca tcacgagatc gcgagccgtt cccggctcgc
     1801 catccgcctg cacgtcgtag ccacgaatga ccgcgacggg ccgcgcgg
//
LOCUS       DASTMD010000491         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_583026, whole genome shotgun sequence.
ACCESSION   DASTMD010000491 DASTMD010000000
VERSION     DASTMD010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583026"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VFQ54_07160"
     CDS             <1..1126
                     /locus_tag="VFQ54_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164809.1"
                     /translation="TGPFKVDSFSPNDQVQFSQNDNYREPNKPFFSGVVLKGGGDAAS
                     AARAVIQTGEYDYAWNLQVEPEILKSMESDSAPGKLMIGPDVTVDRIELNLSDPNKEV
                     NGEKSEMNTPHPFFSDIAVRQAVCTAIDREKLADTFWFGKDGEPAVSNLLEGIPAIQS
                     QANHFEFDTDKANKILDDAGWAKDGKTRSKNDVELKLTYTCSVNQVNAKMQAVVKANL
                     EAIGFNVQLNQVDPNILFDASPGNEQNLQHFYWDMQMFSQSLSSPRPFSYMRGWYAGK
                     DGSNIAQKSNQWVGQNYSRFRNDDYDAALDQAVTETDPDAIAQAFIKMNDIVIQNHVE
                     IPLIRVGQKVGISKKLNADNIALGPFTTQYWNIANWNKVG"
     gene            1228..2880
                     /locus_tag="VFQ54_07165"
     CDS             1228..2880
                     /locus_tag="VFQ54_07165"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU0164810.1"
                     /translation="MAATTPIVDEVSTLNLMNHVRSLAQWVRTSGSAEEAKAFDYLQE
                     QLTSFGYNVTRYESDALIGLPGESLLTIAGENEEIIPSNGYAFGGVTPDAGVSGEIVF
                     VGFGRKDDYAGLDVTGKIVLADGIAMPDKVLKAFEAGAVALININDDHFHEMCISPVW
                     GTPIPETAALLPTLPTLSVLRADGEYLKARIEAGTLTARIVTKPTLTDGKLPTLIGDL
                     PAESATDGTFALFSGHVDSWHYGAMDNGTANATQLEVARLLADRRDDLKRGVRIAFWS
                     GHSHSRYGGSAWYADTFWHELHERCICHVNIDSVGAITADYLTSSGTMAETHAFGAEV
                     VAELTGQTLGYSRMSRSSDQSFWGHGIPSLFAALSQQGGPDDHNPFGLGWWWHTTEDL
                     LDKVNPENLQRDASVFALALWRLCTVDRLPFDYANVANEIDGTLAAYQAQAGDAFDLS
                     GTRDLAQQLATAVAAVNLDAAAFNTLAIELGRILIPVNYTAWGPFEQDLALGTVPLPG
                     LREIVNLPSLSGRDALYLRTKLIRERNRIEHALRTAIRLTEG"
BASE COUNT          616 a         1033 c          947 g          513 t
ORIGIN      
        1 gacggggccg ttcaaggtcg acagcttctc gccgaacgac caggtgcagt tcagccagaa
       61 cgacaactac cgcgagccga ataaaccctt cttctccggg gtcgtcctca agggtggcgg
      121 cgatgccgca tccgccgcgc gagcggtgat ccagacgggt gagtacgact acgcctggaa
      181 cctccaggtc gagcccgaga tcctcaagag catggagtcg gacagcgctc ccgggaagct
      241 catgatcggc cccgacgtca ccgtcgaccg catcgagctc aacctctcgg atccgaacaa
      301 ggaggtcaac ggcgagaagt cggagatgaa tactccccat cccttcttct cggatatcgc
      361 ggtacgccag gcagtctgca ccgcgatcga ccgcgagaag ctggccgaca ccttctggtt
      421 cggcaaggat ggcgagccgg ctgtctccaa tctcctggag ggcatccccg cgatccagtc
      481 gcaggccaac cacttcgagt tcgataccga caaggcgaac aagatcctcg acgatgccgg
      541 ctgggcgaag gacggcaaga cccgctcgaa gaacgatgtc gagttgaagc tcacctacac
      601 ctgcagcgtc aatcaggtga acgccaagat gcaggccgtc gtgaaggcga acctcgaggc
      661 gatcgggttc aatgtccagc tgaaccaggt cgaccccaac atcctcttcg atgcctctcc
      721 gggcaacgag cagaacctcc agcatttcta ctgggacatg cagatgttct cgcagtcgct
      781 ttcctcaccg cgcccgttca gctacatgcg cgggtggtac gccggcaagg acgggtcgaa
      841 catcgcgcag aagtcgaacc agtgggtcgg tcagaactac tcgcgcttcc gcaacgacga
      901 ctatgacgcc gccctcgacc aggccgtcac cgagaccgat cccgacgcga tcgcccaggc
      961 gttcatcaag atgaacgaca tcgtgatcca gaaccacgtc gagatccccc tgattcgcgt
     1021 cggccagaag gtcggcatct cgaagaagct gaacgcggac aacatcgcgc tcggcccctt
     1081 caccacccag tactggaaca tcgccaactg gaacaaagtc gggtaacgtg catcgtctcg
     1141 ttcgtcccgc tttccgagag acgcgcggct ccggccgccg tctctcgtcc gttccggcgt
     1201 cagatccagg aatccgagga gcatggcatg gcagccacca caccgatcgt cgacgaggtt
     1261 tccaccctca atctcatgaa ccatgtccgc tcgctcgcgc aatgggtacg cacctccggc
     1321 agcgcggagg aggcgaaggc attcgactat ctccaggagc agttgacgtc gttcggatac
     1381 aacgtcaccc gctacgaaag cgacgccctg atcggtctgc cgggtgagtc gcttctcacg
     1441 atcgccgggg agaacgagga gatcatcccc tcgaatggct acgcgttcgg cggcgtcaca
     1501 cccgatgccg gcgtgagcgg ggagatcgtc ttcgtcgggt tcgggcgcaa ggatgactac
     1561 gctgggctcg atgtcaccgg caaaatcgtc ctcgcggatg gcatcgcgat gccggacaag
     1621 gtgctgaagg cgttcgaggc tggcgccgtc gcgctgatca acatcaacga cgatcacttc
     1681 cacgagatgt gcatctcccc ggtctggggc acaccgatcc cggaaacggc cgcgctgctc
     1741 ccgacgctgc cgacgctctc cgtgctccgc gccgatggcg aatacctcaa ggcccggatc
     1801 gaagccggca cactcacggc gcggatcgtc accaagccga cgctgaccga cggcaagctg
     1861 ccgaccctga tcggcgacct cccggccgag agcgcgacgg acgggacctt cgccctcttc
     1921 agtggtcacg tcgattcctg gcactacggc gcgatggata acggcaccgc caatgcgacc
     1981 cagctcgagg tcgcgcgcct gctcgcggat cgtcgcgacg acctgaagcg tggggtgcga
     2041 atcgcgttct ggtccggcca ttcgcacagc cgctacggcg gatcggcctg gtacgcggat
     2101 acgttctggc acgaactcca cgagcgttgc atctgccacg tcaacatcga ttcggtcggg
     2161 gcgatcacgg cggactacct cacgagctcc ggcacgatgg cggaaacgca cgcgttcggc
     2221 gccgaagtcg tcgccgaact caccgggcag acactcggct acagccggat gagccgctcg
     2281 agcgaccagt cgttctgggg tcatggcatt ccgtccctct tcgccgccct ctcgcagcag
     2341 ggcggaccgg acgatcacaa tccgttcgga ctgggctggt ggtggcacac gaccgaggat
     2401 ctgctcgaca aggtgaatcc ggagaacctg cagcgggacg cctcggtctt cgcgctcgcc
     2461 ctctggcgac tctgcaccgt ggatcggctt ccgttcgact atgcgaacgt cgcgaacgag
     2521 atcgacggga ccctggcggc gtaccaggcg caggccggcg atgcgttcga tctgagcgga
     2581 acgcgcgacc tggcccagca actcgcgacg gcggtcgcgg cagtgaatct cgacgcggcg
     2641 gcattcaaca ccctcgcgat cgagctcggg cgaatcctga tcccggtcaa ctacacagcc
     2701 tggggaccgt tcgagcagga tctcgcactg ggaaccgtgc cgctgccggg gcttcgcgag
     2761 atcgtgaacc tcccgagcct ctccggacgc gacgcgctct acctccgcac gaaactcatc
     2821 cgcgagcgga accggatcga gcacgcgctg cgcacggcga tccgtctgac ggagggatag
     2881 gctcgacgtt aagaaattcg tagaacgacg gcatcaaccg ttccctatac tgcctcttcg
     2941 tgactgcgcg accggccccg gttcagacag gggccggtcg ccgcgattgt cgatctcgac
     3001 aggcaagatg gtgggtgggg atgacgctgc gggagatgat ccgggaacat aaccagctca
     3061 acggctacct cttcgcgatc atcgagttca cgatccgtcg cggtggcgg
//
LOCUS       DASTMD010000492         5407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_939297, whole genome shotgun sequence.
ACCESSION   DASTMD010000492 DASTMD010000000
VERSION     DASTMD010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939297"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..910)
                     /locus_tag="VFQ54_07170"
     CDS             complement(128..910)
                     /locus_tag="VFQ54_07170"
                     /inference="COORDINATES: protein motif:HMM:NF009389.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0164811.1"
                     /translation="MVGAASDLSLNGKIAIVTGASRRQGIGAAVCRAFASHGADVVFT
                     HWQPYDRVQHYGPDEDGPAALERELRDLGVRAHAVACDLSDPDAPFRLLDQAQELIGS
                     PSILVNNAVHSERNSFRDLDAKALDTAYAVNVRAMALLSVEFARRYPGGPGGRIINMS
                     SGQGVGPMPGELAYVTTKGAIEAFTSTLAAEVAMKGITVNAIDPGATDTGWMTEEFKA
                     AIARELGFGRVGLPEDAARLILFLASDAGAWITGQTIHSRGA"
     gene            1476..2033
                     /locus_tag="VFQ54_07175"
     CDS             1476..2033
                     /locus_tag="VFQ54_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0164812.1"
                     /translation="MRILVTGASSNLASGIIEPLATNHELVLSDIVPVESAHPFVEAD
                     VRKRDEIIAAAKGVDVIIHTPAWHGIQLKTHSEAEFWELNVDGTFNMFQAALANGVKK
                     VIWISSQSVFSNENIYGITKIVGEELCRYYHRVHGISVLMLRPHDFTPIRSHRHYGER
                     LLRGGVDRRDVHQATVLAVDNTTVG"
     gene            2082..2312
                     /locus_tag="VFQ54_07180"
     CDS             2082..2312
                     /locus_tag="VFQ54_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164813.1"
                     /translation="MAWREDAAGVLEKYFPGARDLVANYTLDLPGELTLLDITKEKDV
                     LGYQPRYNFFTFLQDLADHDRQGDGAEWLERP"
     gene            2917..3783
                     /locus_tag="VFQ54_07185"
     CDS             2917..3783
                     /locus_tag="VFQ54_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU0164814.1"
                     /translation="MKSERLRSRIDQHKQSLTKSETVIADRLRKDGIEVAFSTAQAVS
                     AQLGVSEATLVRFARTIGYDSYQHLQQDLQDEIRHQLSITTVERLRDNAAAGSALHEV
                     FKSSLQRDIENLESSLNDFTDADIDAAVDALISARHVYVLGVRASAPAALFLSHSLRY
                     VLETVRPFTLSVESYFEEMLDIGAEDALVVITLSRPSPRALQLVSFAKDRGAKIVAIC
                     DSAHSPIGQLADVALVVESESVTFIQSYTPVMSVGLALIAAVGSARHEDAERRLAAAE
                     ELIERYQLNVKW"
     gene            3800..4750
                     /locus_tag="VFQ54_07190"
     CDS             3800..4750
                     /locus_tag="VFQ54_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164815.1"
                     /translation="MLVRFVIQRILQAIPTLWIITVLIFLLAHLAHGDPVTAMAGPGA
                     SNQLIDEIKAQYHLNRPLPVQYLLWMRDLLHANLGKSITSNQSVLTMIGDRLTLTLLL
                     ALGGTIFSVIIAVPLAILSVMKRGKAIDNIIMAFTSLAISLPNFFTSIVLIVIFGVKW
                     KVLPFAGFPGLREDFWGSVERLILPTISIGLIYLALMTRLVRSELIDVMRADYIRTAR
                     GKGLPDRVVFTVHALRNSMLPSINLITLNFASLLGGTVIIEEVFALPGMGRLIVQAVL
                     QRDFPVIQGVTLVIGLVFILSSIVADVVSYFADPRISASH"
     gene            4758..>5407
                     /locus_tag="VFQ54_07195"
     CDS             4758..>5407
                     /locus_tag="VFQ54_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164816.1"
                     /translation="MNTSVFTSQLPVAATMAAAAPRRDGFWYRFRRDKRAVVSSSILL
                     IITFLAIAAPILPIQSPSAMGLMDLIKTPSRHHLLGTDSFGRDILSRIIYGARVSLLI
                     GLVSVGFALIVGATSGVIAGFYHGPIDAVIMRIMDALLAFPPILLAVALVGVLGPGEF
                     NVMLGLGIVYTPTFARIARAKVMSVSNQEFVTAARAIGISNSRLLFTHVVPNSLGP"
BASE COUNT          939 a         1875 c         1578 g         1015 t
ORIGIN      
        1 ggcggcggca tctcgggacc agaagaatcc gaggtaggac ataaaagtcc caccctacgc
       61 acgaacgctg taggtacgcc ggtcgtgcgt acggcgggac ctccgtgtcc cgcctcggat
      121 cgagaatcta cgcaccacgc gagtggatgg tctggccggt gatccacgcc cccgcatcac
      181 tcgccaggaa caggatcagc cgcgccgcat cctccggcag gcccactcga ccgaagccga
      241 gttcacgcgc gatcgccgcc ttgaactcct ccgtcatcca gccggtgtcc gtcgcaccag
      301 gatcgatcgc gttgaccgtg attcccttca tcgcgacttc cgccgcgagc gtgctcgtga
      361 aggcctcgat cgcgcccttc gttgtcacgt aggcgagctc gcccggcatc ggcccgaccc
      421 cctgaccgga cgacatgttg atgatccgac cgccgggacc acccggataa cggcgcgcga
      481 actcgacgga gagcagcgcc atcgcccgga cgttcaccgc gtaggccgtg tcgagcgcct
      541 tcgcatcgag atcgcggaag ctgttccgct ccgaatggac ggcgttgttg acgaggatcg
      601 acggcgaacc gatcagctcc tgcgcctggt cgaggagacg aaacggcgcg tcgggatcgg
      661 agagatcgca tgcgaccgcg tgggcacgga cgccgagatc gcgcagctcc cgctcgagcg
      721 cggcgggtcc atcctcgtcc ggaccgtaat gctggacgcg atcgtagggc tgccagtgcg
      781 tgaagacgac atcggcaccg tgactggcga acgcgcggca gaccgcggcg ccgattccct
      841 gtcgtcggct ggcaccggtc acgatcgcga tcttgccgtt cagggaaaga tctgacgcag
      901 caccaaccac cttcgcctcc tcgggttctt ccaatgcttt cgctcgaatc tgtctcactg
      961 tacctgattg gcctgccgga atgaccagcg cacggaggct cacagacatc ggtacacccc
     1021 ccttcgatga cctgatgctt acacgacaac accgttgata cagcggtcgt gagtacggcg
     1081 ggacttctat gtcccgcctc cccctcggca aacacgtcgc ttccatctcc cgccgcccca
     1141 gcgcgatcat cgatggggat cgttccggat cgccgttctc tccgaacgga tcggctccgc
     1201 cgacccgggg cgaccacaag agcttgtcct gagcgcagcc gaatggagtc gcgcctacgg
     1261 ttcagcgaac gaccgtaatc ggcgaggcat cgttcatggg acatatccga tgcccgcata
     1321 cggagacatg ggtaacactc gtccgatcgg acaggggttt gacatctccc ggcgctgcgg
     1381 gatgatgcct caatcattga ttagccacgt cccgtgcgta ccatcgccgg gtcacgaacg
     1441 ggaattccgg tcgagttccc cggagagtcg tatccatgcg catcctcgtc accggtgcca
     1501 gcagcaacct cgcgtccggc atcatcgagc cgctcgccac caaccacgag ctcgtgctct
     1561 cggacatcgt gccggtggag tcggcacatc ccttcgtcga ggccgatgtt cgcaagcgcg
     1621 acgagatcat cgccgccgcg aagggggtcg acgtcatcat tcacacgccc gcctggcacg
     1681 gcatccagct caagacgcac agcgaggccg agttctggga actcaatgtc gatggcacct
     1741 tcaacatgtt ccaggccgcc ctcgccaacg gcgtgaagaa ggtgatctgg atctcgagcc
     1801 agtccgtctt ctcgaacgag aacatctacg ggatcacgaa gatcgtcggc gaggaactct
     1861 gccgctacta ccatcgcgtc cacggaatca gcgtcctcat gctgcgcccg cacgacttca
     1921 cgccgatccg ctcccaccgt cactacgggg aacgcctgct caggggcggc gtcgatcgcc
     1981 gcgacgtcca ccaggcgacc gtcctcgccg tcgacaatac gacggtcggt tgagacgggt
     2041 tcggcgtgct gcgcgacgac cccttcaccc aggaggatgc cgtggcgtgg cgggaagatg
     2101 ccgccggtgt cctcgagaag tacttccccg gagcacgcga tctcgtcgcg aactacacgc
     2161 tcgatctccc gggggaactg acgctgctcg acatcacgaa ggagaaggac gtgctgggct
     2221 accagccacg ctacaacttc ttcaccttcc tccaggatct cgccgatcac gaccgccagg
     2281 gtgacggcgc ggaatggctg gagcgcccat aggacggccc atccgggaaa ccgtcccgcc
     2341 aaccgggcaa aatatgggtc gccggaacgg accgcaccgt cgcagatcgt gaatactgcg
     2401 ttgcgttcgt agggcgggac cgccgctgcc ggaccgcagg tccggacggc ctgtcccgcc
     2461 tgctgcgctg acgtccctct gcgccgaacc ggcggcgacc acatgggtcg ccggaacgga
     2521 ccgcaccgtc gcagatcgtg aataccgcgt tgcgttcgta gggcgggacc gccgctgccg
     2581 gaccgcagtc cggacggcct gtcccacctg cgctctgtcc cgcctcccct tgctcgaacg
     2641 agccgtccgc ctgcaatttt tgagcatgat gcccatctgc cacggagaac gacgcgacga
     2701 gtcgcgccgg tgcgtacggt gttggcttct gcgccccagt ttccttcgca tcggttgggc
     2761 aagatgacct atccgggcca catcgccatg tggcagacct gttgacggcc ctcgaaccta
     2821 catgtaacgt ttcttgcaag tcaacgagtg actgaaacat ttcattcttt tcaacctttg
     2881 cccccggcct gcctccatga aggatccctt gcctcgatga agagcgaacg tctccgtagt
     2941 cgcatcgatc agcacaagca gtcgctgacc aagtccgaga cggtcatcgc cgatcgtcta
     3001 cgcaaggatg gaatcgaagt cgcgttctcg accgcccagg cggtaagcgc ccagctcggg
     3061 gtcagtgagg ccacgctcgt ccggttcgcc cgtacgatcg gctacgactc ctaccagcac
     3121 ctgcagcagg atcttcagga cgagatccgc caccagctga gcatcacgac cgtcgagcgg
     3181 ctccgcgaca acgccgccgc cggttccgcc ctgcacgagg tcttcaagtc atcgcttcaa
     3241 cgggacatcg agaatctcga gtcgtcgctc aacgatttca ccgacgccga tatcgatgcc
     3301 gccgtcgacg ccctgatcag tgctcgccac gtctacgtcc tcggtgtccg cgcgtcggca
     3361 ccggcggcgc tcttcctctc gcactcgctt cggtacgtgc tggagacggt tcgtcccttc
     3421 acgctctcgg tcgagagcta cttcgaggag atgctggata tcggggcgga ggacgccctc
     3481 gtcgtcatca ccctctcgcg tccttcgccc cgcgccctcc agctcgtgtc gttcgccaag
     3541 gatcgcggcg ccaagatcgt cgcgatctgc gacagcgccc actcgccgat cggtcagctt
     3601 gcggacgtcg cactggtcgt cgagagcgag tcggtcacct tcattcagtc ctataccccg
     3661 gtgatgagcg tgggcctggc gttgatcgcc gcagtcggct ccgctcgtca cgaagatgcc
     3721 gaacggcggt tggcagccgc ggaagaactc atcgagcgat atcagctcaa cgtgaaatgg
     3781 tgaaggcgga agagacagta tgctcgtacg gtttgtcatt cagcgcatct tgcaagcgat
     3841 tccgaccctc tggatcatca ccgtcctgat cttcctgctg gctcatctcg cccatggcga
     3901 ccccgtgacc gcgatggcgg gtccgggcgc ctccaatcag ctcatcgacg agatcaaggc
     3961 gcaatatcat ctcaatcgtc cgctcccggt tcaatacctg ctctggatgc gcgacctcct
     4021 gcacgccaat ctcgggaagt cgatcacatc caaccagagc gtgctgacga tgatcggcga
     4081 ccgcctgacc ctgacgctgc tgctcgccct cggcggcacg atcttctccg tgatcatcgc
     4141 cgtgccactc gcgatcctct cggtcatgaa gcgcggcaag gcgatcgaca acatcatcat
     4201 ggcgttcacc tcgctcgcga tctcgctgcc gaacttcttc acctcgatcg tgctgatcgt
     4261 gatcttcggc gtgaagtgga aggtgctccc gttcgccgga ttccccgggt tgcgggagga
     4321 cttctgggga tcggtcgagc ggctgatcct gcccacgatc tcgatcgggc tgatctacct
     4381 cgcactgatg acgcgtctcg ttcgcagcga gctgatcgac gtgatgcgcg ccgactacat
     4441 ccgtactgcc cgcggcaagg gattgccgga ccgcgtcgtc ttcacggttc acgccctccg
     4501 gaactcgatg ctgccatcga tcaacctgat cacgctcaac ttcgcgtcac tgctcggcgg
     4561 cacggtcatc atcgaggagg tcttcgccct ccccggcatg ggccgcctca tcgtccaggc
     4621 cgtcctgcaa cgggatttcc ccgtgatcca gggggtcacg ctcgtgatcg ggctggtctt
     4681 cattctctcc agcatcgtcg ctgacgtcgt ctcctacttc gccgatcccc ggatctcggc
     4741 ttctcactag gacatccatg aacacgagcg tcttcacaag ccagctcccg gtcgcggcga
     4801 cgatggccgc cgccgccccg cgccgcgacg gattctggta ccgcttccgg cgagacaagc
     4861 gagccgtcgt cagctcatcc atcctcctga tcatcacctt cctggcgatc gccgccccga
     4921 tcctgccgat ccagtcgccc tccgcgatgg ggctgatgga cctgatcaag acgccgagcc
     4981 ggcatcacct gctcgggacg gattccttcg ggcgcgacat cctgagccgg atcatctacg
     5041 gggcgcgggt ctccctcctg atcggtctcg tctccgtcgg cttcgcgctg atcgtcggcg
     5101 ccaccagcgg ggtcatcgcc ggtttctatc acggcccgat cgacgccgtg atcatgcgga
     5161 tcatggacgc gctgctcgcc tttccgccga tcctgctggc ggtggcgctg gtgggagtgc
     5221 tcggtccggg cgaattcaac gtcatgctcg ggctcggcat cgtctacacc ccgacgttcg
     5281 cccggatcgc ccgggccaag gtgatgtccg tctcgaacca ggagttcgtg acggccgccc
     5341 gcgcgatcgg catctcgaac tcacgattgc tcttcaccca tgtggttccg aattccctgg
     5401 gaccgat
//
LOCUS       DASTMD010000493         1971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1207028, whole genome shotgun sequence.
ACCESSION   DASTMD010000493 DASTMD010000000
VERSION     DASTMD010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207028"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFQ54_07200"
     CDS             <1..827
                     /locus_tag="VFQ54_07200"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0164817.1"
                     /translation="DGIVGGLAASAMRLGRSSVQDYLDSRPVVAELEAIGRAIALAEA
                     TGCSLHIVHVSTGRGAALVGAARARGVDVTAETCPHYLVFTDEDVEQMGAIAKCAPPL
                     RSVADQESLWSAVRGGEIDLIASDHSPSLPSMKSGDDFFAVWGGISGCQHLLAAMISA
                     GIPRGASLPDIARLTSGTIARRFRLAGKGGIAVGNDADLTWLRYRDPVPIPASRVRYR
                     HPASVWDDVPLAVEAAGTIMRGRIVYRDGELLDQPAGRLLKPDVTATRNWPFDERD"
     gene            871..>1971
                     /locus_tag="VFQ54_07205"
     CDS             871..>1971
                     /locus_tag="VFQ54_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU0164818.1"
                     /translation="MRPTWNDYFQPASLGEALNLLHRHGRESRILAGGTDVIVELSRV
                     VKPTNTLIDISRVHELRGIHVDDSSITIGALVTHNDVISSPEIVRDAFPLAQASLEIG
                     APQLRTRATVAGNIATGSPANDTISALIALDADVMLASVDGERVVPIRDFYPGFRQTA
                     LRPDELVRAIRIPRMSARERGVFVKLGLRRAQAISVLHFAIVLDLDGDHVRSARVALG
                     CLAPTVVRGARIEEFLTGRRLTVDACGAAGAIVQDDVAPIDDLRGSGEYRRRALGRLF
                     TDALKRLMLGEERSAWPASPILLDTTSATYALPSGPAGETLEATINGEVRAFPRSSSR
                     QTLLNFLRDEAGLRGAKEGCAEGECGACTVWLE"
BASE COUNT          310 a          640 c          664 g          357 t
ORIGIN      
        1 acgacggcat cgtcggcggt ctggccgcga gcgcgatgcg attgggccgg agcagcgtcc
       61 aggactatct ggacagccgc cccgtcgtcg cggaactcga ggcaatcggg cgtgcgatcg
      121 cgctcgcgga agcaaccgga tgttcgttgc atatcgtcca cgtcagcacc gggcgtggcg
      181 ccgcgctggt tggagcagct cgcgcgcgcg gtgtcgacgt caccgccgag acgtgtccgc
      241 attaccttgt cttcacggat gaggatgtcg agcagatggg tgcgatcgcc aagtgtgcgc
      301 cgccgctgcg gagcgtcgcg gaccaggaga gcctctggag cgccgtccgc gggggagaga
      361 tcgacctgat tgcctccgat cactcgccct cactgccttc gatgaagagc ggggatgatt
      421 tcttcgcggt ctggggtggg atcagtggct gccagcatct gctcgcggcg atgatcagcg
      481 ccggcattcc ccggggtgcg agcctgccgg atatcgctcg cctcacatcc ggcaccatcg
      541 ccaggcgatt ccgcctcgcc ggaaagggcg ggattgccgt cggcaatgat gccgatctga
      601 cctggctgcg ataccgcgac cccgtcccga tccctgcatc ccgcgttcgc taccgtcatc
      661 ccgcctcggt ctgggatgac gtcccactcg cggtcgaggc cgcaggcact atcatgcggg
      721 gacggatcgt ctaccgcgac ggcgagttgc tggaccagcc ggccgggagg ctcctcaagc
      781 ctgatgtgac cgcgacacgg aactggccgt tcgacgagcg cgactgacgg actcgcgata
      841 tctcacggaa aatgcatcga cggggtgccc atgcgaccga cgtggaatga ctactttcag
      901 ccggcatcgc tcggcgaagc attgaatctc cttcatcgcc acggccgcga gtcacggatt
      961 cttgccggcg gcacggacgt gatcgtcgaa ctctcgcgtg ttgtgaagcc caccaacacg
     1021 ctgatcgata tctcgcgggt gcacgagctt cgtggaatcc acgtcgacga ctcgtcgatc
     1081 acgatcggtg cgctcgtcac gcacaacgat gtcatctcgt cgccggagat cgttcgtgat
     1141 gcctttccgc tcgcccaggc gtcgctcgaa atcggcgcgc cgcagctgag gacgcgggcg
     1201 accgtcgccg gaaacatcgc gacgggatcg cctgcgaacg acacgatctc ggcgttgatc
     1261 gctctcgatg ccgacgtcat gctcgccagc gtcgacggcg agcgggtggt gccgattcgc
     1321 gatttctacc ccggatttcg tcagactgcc ctgcgacccg atgaactggt gcgcgcgatc
     1381 cggattcccc ggatgtcggc ccgggaacgc ggcgtcttcg tcaagctcgg gctcaggcgg
     1441 gcgcaggcga tttcggtgct tcacttcgcg atcgtcctcg atctcgacgg ggaccacgtt
     1501 cgctccgccc gcgttgccct cgggtgcctt gcgccaaccg tcgtgcgcgg cgcacggatc
     1561 gaggaattcc tgacgggcag gaggctgacc gtggacgcct gcggtgcggc gggcgcgatc
     1621 gtgcaggatg atgtcgctcc gatcgacgac ctgcgaggaa gcggggaata ccggcgacgt
     1681 gcgctcggtc gccttttcac cgatgcgttg aagcgtctca tgctcgggga ggagcgttcc
     1741 gcctggccgg cgtcgccgat tctccttgat acgacatccg ccacctatgc cctgccgtcg
     1801 ggtccggcag gggaaacgct cgaagcgacg atcaacgggg aggtgcgcgc atttcctcgc
     1861 tcatcctcca ggcagacgct cctcaacttc ctgcgcgacg aggctggact gcggggtgcg
     1921 aaggaggggt gcgccgaggg cgagtgcggt gcctgtaccg tctggctgga g
//
LOCUS       DASTMD010000494         1647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1386694, whole genome shotgun sequence.
ACCESSION   DASTMD010000494 DASTMD010000000
VERSION     DASTMD010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386694"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..>1647
                     /locus_tag="VFQ54_07210"
     CDS             90..>1647
                     /locus_tag="VFQ54_07210"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU0164819.1"
                     /translation="MNQQQPEDGCDVVQPGPDTAQERGASSGSRSRLFRPVNRRVVMK
                     ASAAVSAITMAGGIGAATGLPARTVRAAQNGGTRAGSFVPDDQGSKFSAASEGDFVTF
                     QTDFPFYALGANWGPEVGSWPVIEVQISTDGATWSDTYEMAENHEDGGRPTRGNRFFT
                     PLIFTDGEQYVRYRTVDSNGNAGTVTGLSFTYIDATDGPWAADVDAAVASPDATTFAA
                     ADTGDDRVPPTVVTREEWGADESWRYDSSIGQIWPPEYQTVEHVIVHHTDTPTTQDPI
                     VAIRSIYYYHAVDQGWGDIGYNYLVDRNGRIYEGRYGGQNVIGGHSYEYANGSSGISI
                     IGDYQDSPPSDVSVAGLVAIASWVARKLDPYGTSDFLEAPDLPTICAHRDVNSTTCPG
                     DQLYFDIAEIRSLVAATLNAGDLDSGHPGGIAVRDRIVIQTDDGGPLNLRSNAGTQYS
                     VVAQLANGSPGTVTDGPVSTSSDNWYKILSNGTSGWVVARYLIVSPSAPPASGKFVFG
                     QNIQFTTTTNV"
BASE COUNT          294 a          541 c          549 g          263 t
ORIGIN      
        1 gtgacgcggt cctcgagggg agcatggcga actcgtcatg acgcagggat cgatagcatc
       61 atccgccatc cgatgagggg agtggggcaa tgaatcagca acagccggag gacggctgcg
      121 acgtggtgca gccgggtccg gacacggcgc aggagcgcgg ggcctcgtct ggctcgaggt
      181 cgcggctctt tcgtccggtg aaccggcgag tcgtcatgaa ggcgtcggcc gcggtaagcg
      241 cgatcaccat ggctggtggg atcggcgccg cgacgggtct tcccgcgcga accgtgcgcg
      301 ccgcgcagaa cggcggcacg cgggccggca gtttcgttcc ggacgaccag ggttccaagt
      361 tcagcgccgc gtcggaaggg gacttcgtca cctttcagac ggacttcccg ttctacgcgc
      421 tgggggcaaa ctggggtccc gaggtcggat cgtggccggt catcgaggtg cagatcagca
      481 cggacggcgc gacctggagc gacacctacg agatggcgga gaaccacgag gacggcgggc
      541 gtcccacgcg tggaaatcgc ttcttcaccc cgctgatctt cacggatggc gagcagtatg
      601 tgcgctatcg gacggtggac agcaacggga acgcgggaac ggtgactggt ctctccttca
      661 cctacatcga cgctaccgac ggcccatggg cggcggacgt cgatgcggcc gtcgcgagtc
      721 cggacgcgac cacgtttgcg gccgccgaca cgggcgacga tcgcgtaccg ccgacggtgg
      781 tgacccgcga ggagtggggc gccgacgaat cctggcgcta cgattcgtcg atcggccaga
      841 tctggccccc ggaataccag accgtcgagc acgtcatcgt gcatcacacg gatacgccga
      901 ctacgcagga cccgatcgtc gccatccgct cgatctacta ctatcacgcg gtggatcagg
      961 gctggggcga catcggctac aactacctgg tcgatcgtaa cggacgcatc tacgaggggc
     1021 gctacggcgg ccagaacgtg atcggcggtc actcgtacga atatgcgaac ggcagttccg
     1081 ggatctcgat catcggtgac taccaggact cgccgcccag cgatgtctcc gtcgccgggc
     1141 tcgtcgccat cgcctcctgg gtcgctcgca agctcgatcc ctatggcacc tcggacttcc
     1201 tcgaagcccc cgatctcccg acgatctgcg cccaccggga tgtcaactcg acgacctgcc
     1261 ccggcgacca gctctacttc gacatcgccg agatccgcag cctcgtcgcc gcgaccctga
     1321 atgcggggga cctcgactcc ggccatcccg gcggcatcgc cgtgcgggac cggatcgtca
     1381 tccagaccga cgatggcgga ccgctgaacc tgcgcagcaa cgccgggacc cagtattccg
     1441 tggtggcgca gctcgccaat ggcagcccgg ggacggtgac cgatggtccg gtctccacga
     1501 gcagtgacaa ctggtacaag atcctgtcga acgggaccag tggctgggtg gtcgcgcgat
     1561 acctgatcgt ttcgccctcg gctccgcccg cgagtggcaa gttcgtcttc gggcagaaca
     1621 tccagttcac gacgacgacg aacgtcc
//
LOCUS       DASTMD010000495         5016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1742952, whole genome shotgun sequence.
ACCESSION   DASTMD010000495 DASTMD010000000
VERSION     DASTMD010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742952"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..311)
                     /locus_tag="VFQ54_07215"
     CDS             complement(66..311)
                     /locus_tag="VFQ54_07215"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0164820.1"
                     /translation="MTNSTLVRIETPVGAITVALDGEHTPITTANFLHYIDGGFYTGG
                     RFHRTVRTDNNSNAGLRISDTDGGIDPNADRRQLPND"
     gene            452..1039
                     /locus_tag="VFQ54_07220"
     CDS             452..1039
                     /locus_tag="VFQ54_07220"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEU0164821.1"
                     /translation="MTTPQSALPRPHQPQHTDEHLWHALSEPERRAIRAVFAHRQKVK
                     NVNEIEEEKLTPGERIADSVARVMGSWRFIIIQSVILIGWIFLNALAWIHHWDPYPFI
                     LLNLALSFQAAYSAPIIMMSQNRQATKDRLTAESDYHVNLHSEVEVAAIHGKLDGLIG
                     EQWNNLVELQQKQLELLERIDRLTAQLHQLTSERG"
     gene            complement(1068..2075)
                     /locus_tag="VFQ54_07225"
     CDS             complement(1068..2075)
                     /locus_tag="VFQ54_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU0164822.1"
                     /translation="MQTTRVTFSAANVVELETIDLDETDLGPHEVIVRTHRTLISPGT
                     ELARLQGKLIFDTDEPPAFPMPIVGYANIGTILATGPEVGVSPGDRVYTMGNHASIVR
                     VDTRQMLCVPVPAGLADEDAVFARLATVSMTTLVTTFARPGHAVAIVGLGLVGNLAAQ
                     VFQASGYRVNAFDLSPHRRELAREAGVISVHDGNAMAEFSQQHTLIVEATGSAKALAS
                     AVDMAAPGGEIVMIGAPWGGDANSVPSSQITRLLFYRFLRLRSGSEWEIPRQPEPLAI
                     GSNHQNSVTALDWLSTGKLAVQPLITHRITPADVPDAYAGLRDHPNDYLGVVIDWTGG
                     A"
     gene            2560..3360
                     /locus_tag="VFQ54_07230"
     CDS             2560..3360
                     /locus_tag="VFQ54_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164823.1"
                     /translation="MMERDRGAVVDRVDAIRLGGWQYCPGYEIYWMDMQAKAEPLALV
                     SLVIRSGEHVVLVNCGPDPSQLPPKPAGDPMVTQQHVLESNPDQYLEVQLATLGLSPA
                     DVTHVICTPFQIYSLGNLRKLTNAEICLSRTGWIDFHAPRWKEHPHDKRWSCIPRDVL
                     VNLVTDAWPRVRLVHDEVIVPGVETFWTGVHHRASLAVKIQTTRGIVIAGDAFMRLGN
                     IERRHPIGINESMEEALTTYDRVAREADIIVPLYDPAVWERHPGGRIA"
     gene            3392..3976
                     /locus_tag="VFQ54_07235"
     CDS             3392..3976
                     /locus_tag="VFQ54_07235"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEU0164824.1"
                     /translation="MSSRTIEIVRYDETWPDQFREIAVPLRSALGEIAVRIDHIGSTS
                     VPGLAAKDVIDVQVTVATLDGPLIAGLLAPVGYTRRADINHDHVPPGEVTATAEWEKL
                     YFRAPEGQRPTHLHVRQNGRANQRYPLLFRDYLRANADASETYRQIKVALARLHPDDV
                     EAYYDIKDPACDLIMQAATRWVRESGYTIGATDA"
     gene            complement(4105..>5016)
                     /locus_tag="VFQ54_07240"
     CDS             complement(4105..>5016)
                     /locus_tag="VFQ54_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164825.1"
                     /translation="DQVSQPIVQTAQVSAADTGATPSDRAFELYLNGDFNATPYTFST
                     VNGVAVVDGLATGSYTLVDVQTLAQFPGIVIQSNTTTSIIAIFPSGPAAGDSDVASLA
                     ISAFYTDSVNQPTSVLSAPLSGDGSGTPVAGAAAVDPADIGQNVTPADRNFSIYLNGD
                     LDSVPFATVETVGGSAVVNNLPVGTHAIVDDETGTAFSFTIASGSITAITLYFPNGSP
                     AVVPTTAPGAPTVAPTTAPGGGTSGGGSSTGGGSSASGSTSVTALPNTGQGTESHSTS
                     TAFLMLLGSISLIVIAAGITWRRRQSL"
BASE COUNT          928 a         1512 c         1694 g          882 t
ORIGIN      
        1 atcggaccgg cctgctcgcc gatgcgatcg ggattgacgc cgccctggat cacctcgatc
       61 acgatctagt cgttcggcag ctggcgacga tcggcgttcg gatcgatccc accgtcggta
      121 tcgctgatcc ggagcccggc gttcgagttg ttgtccgtgc ggacggtgcg gtgaaagcga
      181 ccgccggtgt agaacccacc gtcgatgtag tggaggaagt tcgccgtggt gatcggcgtg
      241 tgctcgccgt cgagggcgac cgtgatcgcg ccgaccggtg tctcgattcg aaccagcgtg
      301 gaattcgtca tcttcgaagg tatctccagg ttccaggttg cgtacatcac atcagcatga
      361 cgtgcgagat cgtatcctgc ctgtgtcgat cggcgcaatt cccaaccggc agagagcacg
      421 tgatgtcgac gacagaatca ggagcctgat ggtgaccacc ccccaatccg cactgccccg
      481 acctcatcaa ccgcaacaca ccgatgagca tctctggcat gcactgagcg agccggagcg
      541 acgggcgatc cgcgccgtct tcgcacaccg gcagaaggtc aagaatgtca acgagatcga
      601 ggaagagaag ctgacaccgg gtgagcgaat cgcggattcc gtcgcccggg tgatgggatc
      661 ctggcgattc atcatcattc agtcggtgat cctgatcggg tggatcttcc tcaacgcgct
      721 ggcgtggatt caccactggg atccctatcc tttcattctc ctcaacctcg cgctctcgtt
      781 ccaggcggcg tactcggcgc cgatcatcat gatgagccag aaccggcagg cgacgaagga
      841 tcgactgacg gcggagtccg actatcacgt caatctccac tccgaggtcg aggtcgcggc
      901 gatccacggc aagctcgatg gcttgatcgg ggagcagtgg aacaacctcg tcgagttgca
      961 gcagaagcaa ctggaactgc tcgagcggat cgatcgcctc acggcgcaac tgcatcagct
     1021 gacgagcgag aggggatgac gcgtcgtcgc gttcgggcga agaggactca agctcccccg
     1081 gtccagtcga tgacgacgcc aaggtagtcg ttgggatgat cccgcaaccc ggcgtaggcg
     1141 tccggaacgt ccgccggtgt gatgcgatgg gtgatcagcg gctgtaccgc gagcttgccc
     1201 gttgagagcc agtcgagggc ggtgaccgag ttctgatgat tggagccgat cgccaggggt
     1261 tccggctggc gggggatctc ccactcggaa ccgctgcgga ggcggaggaa gcgatagaag
     1321 aggagtcgcg tgatctggct cgagggcacg ctgttcgcgt cgccgcccca cggtgcgccg
     1381 atcattacga tctcgccgcc gggcgccgcc atgtcgaccg cgcttgccag tgccttcgcg
     1441 gagccggtcg cctcgacgat cagcgtgtgc tgctgactga actccgccat cgcattgcca
     1501 tcgtgaacgg atatgacccc ggcttcccgg gcgagctcgc ggcggtgcgg ggagagatcg
     1561 aaggcgttga cgcgatagcc gctcgcctgg aagacctggg ccgcgagatt cccgaccagg
     1621 ccgagcccga cgatcgcgac cgcgtgaccg ggccgggcga aggtcgtcac cagtgtcgtc
     1681 atcgaaaccg tggcgagacg cgcgaagacg gcgtcctcat cggcgaggcc ggctggtacc
     1741 ggcacgcaga gcatctgccg ggtatcgacg cggacgatcg aggcgtgatt ccccatcgtg
     1801 tagacgcgat cgccgggact gacgccaacc tcgggtccgg tggcgaggat cgtcccgatg
     1861 ttcgcgtagc cgacgatcgg catcgggaac gcgggcggct catccgtgtc gaagatgagc
     1921 ttgccctgaa ggcgggcgag ttccgtgccc gggctgatca gggttcgatg cgtgcgcacg
     1981 atgacctcgt gcggaccgag gtcggtctca tcgagatcga tcgtctcgag ttcgacgaca
     2041 ttggcggcgc tgaacgtaac gcgcgttgtc tgcatgtgcg ctcctggcgc cgcgtctcga
     2101 ggcgggcccg cttgggaagg tgtggatgat ttcattgatt atccacgcct gggtggggac
     2161 atagaagccc ccgccacaat gcggattcgg aagaaacatg ccccgaggtg acgaccgtgg
     2221 cggttgcatt cccatcccca cggcgcttgt cgccgtttcg ggtcggtgcc gcgacgccga
     2281 cccgcttgcg aacgatcatt cgtcgcgaga cctcatgtac gggacgttcg tgttcgcggc
     2341 ggggtataaa accccgccct accaaccccg gcggtcgatg cgatcgggat tgccacgcgt
     2401 atcgcgttga tcgagttgaa cagcacgatc gcgtggattt gtcgttgccc gggtggttga
     2461 cgataccagc cgtgcggcga gacggcatgt gttcgcgcgg ggccatgagc gagaatgaac
     2521 caaattcgca ggcgcataag aggaaagcag gagggatcca tgatggaacg ggatcgtggc
     2581 gcggtcgtcg atcgggtcga cgcgattcgg ctgggaggct ggcagtactg ccccggctac
     2641 gagatctact ggatggacat gcaggcgaag gccgagccgc tggcgctcgt cagcctcgtg
     2701 atccggagcg gtgaacacgt cgtgctcgtc aattgcggtc ccgatccgtc gcagttgccg
     2761 ccgaagccgg cgggtgatcc gatggtgacg cagcagcacg ttctcgaatc aaatccggat
     2821 cagtacctgg aggtccagct cgcgacgctc gggctgtcac cggcggatgt gacgcacgtc
     2881 atctgtacgc ctttccagat ctactcgctc ggcaacctac gcaagctgac gaatgccgag
     2941 atctgtctct cccggaccgg atggatcgac ttccacgccc cgcggtggaa ggagcatccc
     3001 cacgacaagc gctggagctg catcccgcgg gatgtgctcg tcaacctcgt gaccgatgcc
     3061 tggccgcgcg ttcgcctcgt gcacgacgag gtgatcgtgc cgggcgtcga gacgttctgg
     3121 acgggtgtcc atcaccgggc aagcctggcg gtgaagatcc agacgacaag gggcatcgtg
     3181 atcgcgggag acgccttcat gcgactcggg aatatcgagc gccggcatcc gatcgggatc
     3241 aatgagagca tggaagaggc gctgacgacc tacgaccggg ttgcgcggga ggcggatatc
     3301 atcgtcccgc tctacgatcc ggccgtctgg gagcgtcatc ccggtggacg catcgcgtaa
     3361 ctggccgtga tggtgcggaa aggggacacc gatgagttcg aggacgatcg agatcgtgcg
     3421 gtatgacgag acgtggccgg atcaattcag ggagatcgcg gtcccgttgc gatcggcact
     3481 gggtgaaatc gcggtcagga tcgaccacat cggatcgacc tcggtgcccg gtctcgccgc
     3541 aaaggatgtg atcgatgtgc aggtcacggt ggcgacgctg gacggtccgc tgatcgccgg
     3601 gttgctcgcc cccgtcggct atacgcgacg ggccgacatc aatcacgatc atgtcccgcc
     3661 gggcgaggtt accgccaccg ccgaatggga gaagctctac ttccgcgccc cggagggaca
     3721 gcgaccgacg catctgcacg ttcgccagaa cgggcgggcc aaccagcgct atccgttgct
     3781 ctttcgcgac tatcttcgcg caaatgccga tgcgtccgag acgtaccggc agatcaaggt
     3841 tgcgctggcc cgcctccatc cggacgatgt cgaggcgtac tacgacatca aggatcccgc
     3901 ttgcgatctc atcatgcagg cagcgacgcg gtgggtgcgg gagtccggct ataccatcgg
     3961 cgcgaccgac gcctgaagcc aatcgccgcc gttgcgatcc cgggacaaac gggcagaacc
     4021 tcctggcgat ttgccagaag gtcctgctcg ttcgatcaga tgtgggtctc cgatcgacga
     4081 cgatttacat ggtctcccaa gacattacag cgactggcgt cggcgccagg tgatgccagc
     4141 ggcgatgacg atcaggctga tcgagccgag aagcatcagg aacgccgtcg aggtgctgtg
     4201 gctttccgtg ccctgaccgg tgttcggaag tgccgtcacc gaggtgctgc cactcgccga
     4261 cgaaccgccg ccggtcgagc taccgccacc cgaggtgcca ccacccggag cggtggttgg
     4321 agccacggtc ggcgcacccg gagcagttgt cgggacgacc gccggtgaac cattcgggaa
     4381 gtaaagcgtg attgccgtaa tgctgccgga cgcgatcgtg aaggagaagg ctgtgccggt
     4441 ctcatcatcg acgatcgcat gggtgccaac cggcaggttg ttcaccacgg cgctgccgcc
     4501 gacggtctcc acggttgcga acggaacgga atcgaggtca ccgttgaggt agatcgagaa
     4561 gttccgatcc gccggggtca cgttctggcc gatgtccgcc ggatcgaccg ccgccgcgcc
     4621 ggcgaccggc gtgcccgagc cgtcaccgga gaggggagca gagagcaccg acgtcggctg
     4681 gttcacggaa tccgtgtaga acgccgagat cgcgagcgat gcaacatcgg aatcacccgc
     4741 ggccggaccg gacgggaaga tcgcgatgat cgaggtcgtg gtgttcgact ggatgacgat
     4801 gcccgggaac tgggccagcg tctggacgtc cacgagcgtg tacgagccag tggcgagacc
     4861 gtcaaccacc gcgacaccgt tgacggtgct gaaggtgtac ggcgtggcgt tgaagtcgcc
     4921 gttcaggtag agctcgaagg cgcgatcgga cggtgtcgcc ccggtatcgg cggccgagac
     4981 ctgggcggtc tggacgattg gttgcgagac ctgatc
//
LOCUS       DASTMD010000496         1700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_404113, whole genome shotgun sequence.
ACCESSION   DASTMD010000496 DASTMD010000000
VERSION     DASTMD010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404113"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /gene="ppk1"
                     /locus_tag="VFQ54_07245"
     CDS             complement(<1..1307)
                     /gene="ppk1"
                     /locus_tag="VFQ54_07245"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEU0164826.1"
                     /translation="MTRPAHIVRLAHDDDDPFESGEGGDRFFNRELSWLAFNRRVLEE
                     ASNEAHPLLERVRFLSISGSNVDEFFMVRVAGLMGQQQQDVERRSPDGLTPREQLTAI
                     VANADELVEDQRNVWRELRRLLAETGIQVLGSRQIDEAVNEEEGAWLENYFREQIFPI
                     LTPQALDPAHPFPFMPNKGLAVIFDLVRASDGEEIRELVMLPARMARFVRLPGEPARY
                     FAVESLVKRFSTVIFPGYAVTGAAEFRVLRDSDIETEEEAEDLVRYFANAIKRRRRGR
                     VIRLELESGMPDNLATILKDELAGGDAFVTESGAFLGISDLDAIIDENRPDLKFVPFS
                     PRFPERIREFGGDCFAAIKSKDIVVHHPYETFEVVIEFLKQAAADPDVVAIKQTLYRA
                     GKQSAVINALIAAAESGKSVTAVVELKARFDEEQNLLWASALER"
     gene            complement(1304..>1700)
                     /locus_tag="VFQ54_07250"
     CDS             complement(1304..>1700)
                     /locus_tag="VFQ54_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164827.1"
                     /translation="EADLFHAWNAAQAERRPQLFLADVAPPEWRVKLPDLRSRLAASP
                     VAVIEAPDDMLMAALLERQFHRRGLDARPDLIQWLVARLERSHIAVIRAVDILDQDVL
                     ERRKRLSIPLARATLVEAGIIHPPTEETR"
BASE COUNT          300 a          561 c          535 g          304 t
ORIGIN      
        1 cgctccagcg cggaggccca gagcaaattc tgctcctcat cgaagcgggc tttcagctcg
       61 acaaccgccg tcaccgattt gcccgattcg gccgcggcga tcagcgcatt gatcaccgcc
      121 gattgcttgc ccgcgcggta gagcgtctgc ttgatcgcga cgacatcggg atcggcggcg
      181 gcttgcttga ggaattcgat caccacctcg aacgtttcgt agggatggtg gacgacgata
      241 tccttggatt tgatcgcggc gaagcaatcg ccgccgaatt cgcgaatccg ctccgggaag
      301 cgcgggctga acggcacgaa cttcagatca gggcgattct cgtcgatgat cgcgtcgagg
      361 tcggagatgc ccaggaacgc gccgctttcg gtgacgaaag catccccgcc ggccagctcg
      421 tccttcagga tcgtcgccag attgtcgggc atgcccgatt cgagctccag ccggatcacc
      481 cgcccgcgcc gccgccgctt gatggcgttg gcgaagtaac ggacgaggtc ctcggcctct
      541 tcctcggtct cgatatcgct gtcgcgcagc acccggaact cggctgcgcc ggttaccgca
      601 tagcccggga agatgactgt cgagaatcgc tttaccagcg attcgaccgc gaagtaacgt
      661 gcgggctcgc ccggcaagcg gacgaaccga gccattcgcg cgggcagcat caccagctcc
      721 cgaatctcct ccccgtccga cgcgcggacc aggtcgaaga tcaccgccag tcccttgttg
      781 ggcatgaacg ggaacggatg cgccggatcg agcgcctggg gcgtcaggat ggggaaaatc
      841 tgttcgcgga aatagttttc gagccaggcg ccctcttcct cgttcacggc ttcatcgatc
      901 tggcgcgacc ccagcacttg aatgccggtt tccgcgagca agcggcgcag ctcacgccac
      961 acgttacgct ggtcctcgac cagctcgtcg gcattggcga cgatcgcggt cagctgttcg
     1021 cggggggtca ggccatcggg cgagcgccgt tcgacgtcct gctgctgctg gcccatcaag
     1081 ccggcgaccc ggaccatgaa gaactcatcg acgttcgatc cggaaatcga caggaaccgg
     1141 acgcgttcca gcaatgggtg cgcctcgttg ctcgcttcct ccagcacgcg gcggttgaac
     1201 gccagccacg acagttcgcg gttgaagaag cgatctccac cctctcccga ttcgaagggg
     1261 tcgtcgtcat catgagccag gcgcacgata tgggctgggc gagtcatcgg gtctcttccg
     1321 ttggcggatg gatgataccc gcctccacca atgtggcgcg ggcgagcggg atcgacaagc
     1381 gtttgcgccg ttcgagcacg tcctggtcga gtatatcgac cgctcggatg acagcgatgt
     1441 gacttcgctc cagccgcgcc accagccatt ggatcagatc gggtcgggca tcgagcccgc
     1501 ggcgatggaa ttgccgttcg agcagggccg ccatcaacat atcatcgggc gcctcgatca
     1561 ccgccaccgg gctggcggcg agccgggatc gcaggtcggg caacttcact cgccactccg
     1621 gcggcgcgac atccgccagg aacagctgcg gccgccgctc cgcctgcgcg gcgttccagg
     1681 cgtggaacag gtccgcttcc
//
LOCUS       DASTMD010000497         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2724821, whole genome shotgun sequence.
ACCESSION   DASTMD010000497 DASTMD010000000
VERSION     DASTMD010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2724821"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..>1527
                     /locus_tag="VFQ54_07255"
     CDS             186..>1527
                     /locus_tag="VFQ54_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0164828.1"
                     /translation="MTPTSQSPRTIVPTFEAHVPLIVVGGGLAGTFAAIAAARNGTRV
                     VLIQERPVLGGNSSSEIRVHPVGASQHGYHRDARETGLMEELFIEVRARAYGLRQING
                     RHYPMWDVILAEMAEAEPNLTLHLNTRVIGVETEDDIRDGYTTRITGVTAVQQGTEAV
                     FHYLADAAIDATGDGFIALQAGAPFRYGRESRAEFDEQWAPEEADDIVLGSTIMFAAR
                     DVGRPVPYAPPAWAHSFPDEDSLPFRAHEELDSGYWWIEWGGKLDTIVDNEAIRKELH
                     AAVFGVWDHIKNHCTVPGVRERAANMTLDWIGHIPGKRESRRFEGDYIITEKDITHGI
                     FDVPDDVVAYGGWAIDLHAPDGVYSPDHPCTQPPLPDLYGIPLRSLYSRTVSNLFLAG
                     RNISQTHVAHGSTRVMKTTAVIGEAAGTAAAFAIAGDQTPRALVADATRLQALQQ"
BASE COUNT          278 a          527 c          480 g          242 t
ORIGIN      
        1 gacgccgcaa ctgaccacga ttcgggtcga tcgcgtttcc tggggcgccc gcgccatgga
       61 ccgcctgatc gacacgctcg aaggaatcaa accgctcggt tcggaccgac tgctgatgcc
      121 ggtcgagctg atcgttcgcg catcttccgg cccggtttcg catccgtagt tcccaggagc
      181 agatgatgac cccgaccagc caatctcccc ggacgatcgt ccccacgttc gaagcgcacg
      241 tcccgctcat cgtcgtcggc ggtggactgg ccgggacgtt cgccgcgatc gccgcggctc
      301 gcaacgggac gagggtcgtg ctgattcagg agcgtcctgt gctcggggga aacagcagca
      361 gcgagatacg agtccacccc gtcggcgcgt cgcagcatgg ctatcaccgc gacgcccgcg
      421 agaccggatt gatggaggaa ctcttcatcg aggtccgtgc gcgcgcctat ggactgcgcc
      481 agatcaacgg ccgccattac ccgatgtggg atgtgattct cgccgagatg gcggaggcgg
      541 aaccgaacct cacgctccat ctcaacacgc gcgtgatcgg cgtcgagacg gaggatgaca
      601 tccgcgacgg ttacacgacc cggatcaccg gcgtcacggc ggttcagcag gggacggaag
      661 ctgtcttcca ctacctcgcg gatgccgcga tcgacgcgac cggcgacggc ttcatcgccc
      721 tccaggcggg cgcgccgttc cgatacggac gggagagccg cgcggaattc gacgaacagt
      781 gggcgccgga ggaagccgac gacatcgtgc tcggctcgac gatcatgttc gccgctcgcg
      841 atgtcggacg gcccgtcccc tacgcgccac cggcatgggc gcactcgttc ccggacgagg
      901 attccctccc cttccgcgcg cacgaggaac tcgacagcgg ctactggtgg atcgaatggg
      961 gcggcaagct cgacacgatc gtcgacaacg aggcgatccg gaaggaactc cacgcggcgg
     1021 tcttcggcgt ctgggatcac atcaagaacc attgcaccgt accgggagtt cgcgagcgcg
     1081 ccgccaacat gacgctggac tggatcggtc atatccccgg gaaacgcgag agccgccgtt
     1141 tcgagggtga ctacatcatt acggagaagg acatcaccca cggcatcttc gacgtgccgg
     1201 atgatgtcgt cgcctatggc ggctgggcga tcgacctcca cgcgccggac ggcgtctact
     1261 cacccgatca cccgtgcacg caaccgccgc tgccggatct ctacggcatc ccgctccgtt
     1321 cgctgtatag ccggacggtg agcaatctct tcctcgctgg gcgcaacatc agccagactc
     1381 acgtcgccca cggctcgacc cgggtcatga aaacgaccgc cgtgatcggc gaagccgccg
     1441 gtaccgctgc cgccttcgcg atcgccggag atcagacacc gcgcgcactc gttgccgacg
     1501 cgacgcgcct ccaggccctg cagcagc
//
LOCUS       DASTMD010000498         1570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_134361, whole genome shotgun sequence.
ACCESSION   DASTMD010000498 DASTMD010000000
VERSION     DASTMD010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134361"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..872)
                     /locus_tag="VFQ54_07260"
     CDS             complement(309..872)
                     /locus_tag="VFQ54_07260"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEU0164829.1"
                     /translation="MVREGEVFFDVAYPEHLSRGLIFIKWLLIIPHIIVLWVLSVVAS
                     VVYVIGWFAVLILGRYPVGMWNFATMVLRWGMRVNVYTSLMRDEYPPFGDASYPIRFD
                     VLYPQRLSRWKIFFKWLFVIPHMIALYVLGIIFSIVSVIAWFAILITGRYPQALFDFS
                     VGVLRWGLRVSMYLFCLTDVYPPFSLR"
     gene            complement(1176..>1570)
                     /locus_tag="VFQ54_07265"
     CDS             complement(1176..>1570)
                     /locus_tag="VFQ54_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164830.1"
                     /translation="ITVPDTLPDSLAGQLDPDTIAAENKKLVTYTPKKPTPTPAPTAK
                     PKAKVKAKPTITSTPTPTPKAKVKAKPTATPTPTPKAKVKAKPTVTPTPTATPAGSGT
                     PTRKATPAAGDVAVQHVAGIDDRRRLLA"
BASE COUNT          316 a          455 c          488 g          311 t
ORIGIN      
        1 gtcgtggatg cggcttcgga gatgatgtcg accgcgcgac gaacgcccca gcaatagccg
       61 aggacatccg cgacgataac ccgtcgctca ggctgggtga ccggtgtggt tgtggatgat
      121 tctgtaggga cggcgatggc catgagattg atcctcgatc cgggctgggt ggatgcgcgg
      181 ccagttcatt cccgtcacgc cgacggcgaa caggctccgc aggcagccga tgcatcatgg
      241 cggcgttctc acaccacctc gtacaagtat agccccagat cccggacgat tcttcgatca
      301 gtgcaggatt atctcaacga aaacggcgga tagacatcgg tgaggcagaa caggtacatg
      361 ctcacccgaa gtccccagcg caggacgccg acgctgaaat cgaagagcgc ctgcggatat
      421 cgtccagtga tgagaatcgc gaaccaggcg atgacgctca caatggaaaa gatgatcccg
      481 agcacgtaga gcgcgatcat gtgtgggatc acgaagagcc acttgaagaa gatcttccat
      541 cgcgaaagac gctggggata gagcacgtcg aatcggatcg gataggacgc atcgccgaac
      601 ggcgggtatt cgtcccgcat gagcgacgta tagacattga cccgcatccc ccagcgcagc
      661 accatcgtcg cgaagttcca catccccacg gggtagcgac cgagaatgag cacggcgaac
      721 cagccaataa catagaccac gctggccaca acgctgagga cccacaacac gatgatatgt
      781 ggaatgatca acagccactt gatgaagatc aggccacggc tcagatgttc cgggtaggcg
      841 acatcgaaga acacttcccc ctcgcggacc atagcactcc ccatttccga atatacgttc
      901 ggcgaacgtc acctcgcctt ttgtcaggac gcccagaatc tacaaataac cggattgcga
      961 tgctcattgg tatcacccga tcggatgaat gttccgcaat tgaatcaaga accggccaat
     1021 catgaccgag acaggggttt cgaactgtga cttggggatg attgatccat gttcggcttg
     1081 tgccgggccg ttcctcgccg ctttttcgac agctgtcgag gcgacctggg ggcaaggcag
     1141 ccggctccac ttctgtcgcc gatcgctggt ccgaatcagg cgaggagacg gcgccgatcg
     1201 tcgatgcccg ccacgtgctg caccgcgacg tcgccggccg cgggcgttgc ctttcgggtg
     1261 ggcgtcccgc tcccggcggg cgttgcggtc ggggttgggg taacggtcgg ctttgccttc
     1321 accttcgcct tgggcgtcgg ggttggggta gcagttggct ttgccttcac cttcgccttg
     1381 ggcgtcggtg tcggagtcga ggtgatggtt ggcttcgctt tcaccttcgc cttgggtttc
     1441 gccgtcggcg ctggcgtcgg cgtcggcttt ttcggcgtgt aggtgacgag cttcttgttc
     1501 tccgccgcga tcgtgtccgg atcgagctgc cccgcgaggg aatccggcag ggtgtccggt
     1561 acggtgatca
//
LOCUS       DASTMD010000499         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2724970, whole genome shotgun sequence.
ACCESSION   DASTMD010000499 DASTMD010000000
VERSION     DASTMD010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2724970"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VFQ54_07270"
     CDS             <1..1051
                     /locus_tag="VFQ54_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164831.1"
                     /translation="RAITLISTVGLVGLAIGPATGGFVLAIAPWQVLLLVNVPIAALA
                     FIGIRSGIAADEAADLHRDPVDIAGAILGTATIVLALVAPTLFVNDGTGSWAPWTVTA
                     AAIVGAMLFILRERAARYPLLDFKLVAQPLVASGLAYKAASGLAIAGLGYMVTLQLQF
                     AWGWSPAHAAIGTLPQVVVLVAGSSVVGPFIQRVGFDRAAWLSAAAVVFGLAIFGLFS
                     RFGYVWVAIALVLAAAGMRVVGVVAGNNVLRGLPENRTTIGAALVDTASEVTSGVGIA
                     VTGTILAALFTGNITASNWSTHQTSEFEAAVTTAGLVLATVAAALVGWAILRTRNASG
                     GRATTSVDDAIPERA"
     gene            1246..1620
                     /locus_tag="VFQ54_07275"
     CDS             1246..1620
                     /locus_tag="VFQ54_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU0164832.1"
                     /translation="MATIDDARRITRSFPETTEDPEGFGFSVRDKGFAWAWTERVEPK
                     KPRVRRDDVLAVRVSGDAEKQILLASDPVKFFTEPHYNGFPAILVNLPAIDDEELAEL
                     LLNAWRCRAPKRLVQEMDGSAG"
     gene            1754..>2699
                     /locus_tag="VFQ54_07280"
     CDS             1754..>2699
                     /locus_tag="VFQ54_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0164833.1"
                     /translation="MRDPQMIGKDAPQSMDEVLATQPRREHVTISLSIKTIGIVFGIL
                     LTIWVLKELSGTLLIFAGAILLATAIDRPVSWMQSRGIPRGFGITIIFAGVVGLLVGV
                     VAVLIPLVTDETVHFKDHLSDYQRQFEDELNKFGIKSSENTHQISLDQITSRIGDNVS
                     TIASSLTSITIGIGHTAVLIFAMLVISFMLAMDPTSGTRFARRFLTDSAHARMQRISG
                     DIHDRIGGWVRGQVLVAATFGIAFGLGLWIIGVPYAASLGLAAGVLEVMPYLGGAITV
                     VIAAIVALTIGVPQLVAVLVLYAILINIESHILAPRFIG"
BASE COUNT          442 a          902 c          835 g          520 t
ORIGIN      
        1 ccgggcgatc accctcatct cgaccgtcgg tctggttggt ctggcaattg gtccggcgac
       61 gggtggcttc gtgctcgcca tcgctccatg gcaggtcctg ctgctcgtga acgtaccgat
      121 cgccgcgctc gcgttcatcg gcatacgatc cgggatcgcc gcggacgagg cagccgacct
      181 gcatcgcgat cccgtcgata ttgccggcgc catcctcggc acggcaacga tcgtgctcgc
      241 gcttgttgca ccgaccctct ttgtgaacga cggtaccggc tcctgggcgc cgtggacggt
      301 caccgcagcg gccatcgtcg gggcgatgct cttcatactc cgcgagcgcg cggcccgtta
      361 cccgctgctc gacttcaagc tggtcgcgca accactcgtc gccagcggtc tcgcctacaa
      421 ggctgcctcc gggctcgcta tcgcgggtct cggctacatg gtgacgctcc agctgcagtt
      481 cgcatggggt tggtcaccgg cacacgcggc gatcgggacg ctgccccagg ttgttgtcct
      541 cgtcgcgggc agctcggtcg tcggtccgtt catacaacgg gtaggcttcg atcgggcggc
      601 atggctgagt gccgcggcag ttgtgttcgg tctcgccatc ttcgggcttt tcagccgctt
      661 cggatacgtg tgggtcgcga tcgcactcgt cctcgcggct gccgggatgc gcgtcgtcgg
      721 cgttgtcgcc ggaaacaacg tgctccgtgg tctgccggag aaccggacga cgattggcgc
      781 ggcgctcgtc gatactgcta gcgaggtcac ctccggcgtc ggtatcgccg tcaccggaac
      841 catcctcgcc gcgctcttca ctggcaatat cacagcatcg aactggagca cgcaccagac
      901 ctcggaattc gaagcagcgg tcacgactgc tggactggtg cttgccaccg tggctgccgc
      961 gctcgtaggg tgggcgattc tgcggacgag gaacgcctcg ggcgggcggg caacaacgtc
     1021 cgttgacgac gcgattcccg agcgggcctg accgccgccg attcgtcctc cgggagcagc
     1081 gaatgggagt tcccgacaag cttcaaatga ctgcccgtcc gtgagtcccc tgagggatac
     1141 ggtccggatc gccatcctca tcgccgattt cgggtgactg gccccgattc tttcatgcat
     1201 catgatcgga acagttccgc ttgtcccacc ggaaaggctg gaggaatggc aacgatcgac
     1261 gatgcccggc gaatcacgcg ttcgttcccc gagacgacag aggaccccga gggctttggc
     1321 ttcagcgtga gggacaaggg attcgcctgg gcctggacgg aacgggtcga gccgaagaag
     1381 ccgcgagtcc ggcgggacga tgtcctcgcc gtccgcgtat cgggtgacgc cgagaagcag
     1441 atcctgctgg ccagcgatcc agtgaagttc ttcaccgaac cccactacaa cggattccct
     1501 gccatcctcg tcaacctccc ggcgatcgac gacgaggagc tcgcggagct gctcctcaac
     1561 gcctggcgct gccgcgcacc aaaacgactc gtgcaggaga tggacggctc cgccgggtga
     1621 atcatcttct cgttgaaggt cgagtcgggc ccaatctggc gaatcgctgt gtaacgcggt
     1681 atcgtcgcgc atgcgcgatg gtgcgcgatg aacacagccc ggcggcccga cgctggggag
     1741 gagcaagccg gccgtgcgcg atccccagat gatcgggaaa gatgctccac aatcgatgga
     1801 cgaggtcctc gccacccagc cccggcgcga acacgtcacg atcagcctgt cgatcaagac
     1861 gatcggcatc gttttcggca tcctcctcac gatctgggtc ctcaaggaac tctccggcac
     1921 gctcctcatc ttcgccggtg cgattctgct cgccacagcc atcgatcgtc ccgtgtcctg
     1981 gatgcagtct cgtggcattc cgcgcggctt tggcataacg atcatctttg cgggcgtcgt
     2041 cggactcctg gtcggggtag tcgcggtcct catccccctc gtgaccgacg agacggtgca
     2101 cttcaaggac cacctcagtg actatcagcg tcagttcgag gacgaactga acaagttcgg
     2161 catcaagtcc tcggaaaaca cgcaccagat atcgctcgac cagatcacga gccggatcgg
     2221 cgacaacgtc tccaccatcg cctcgagcct gacgtcgatc acgatcggca tcgggcacac
     2281 cgccgtcctg atcttcgcga tgctcgtcat ctccttcatg ctggcgatgg atccgacgtc
     2341 gggaacccgg ttcgcccggc gcttcctgac cgacagcgcc cacgcccgga tgcaacgcat
     2401 ctccggcgat atccacgatc ggatcggcgg ctgggtgcgc ggccaggtac tggtcgccgc
     2461 gacgttcgga atcgccttcg ggctcgggtt gtggatcatc ggagtgccct acgcggcgtc
     2521 gctcggcctc gcggccggcg tcctcgaggt aatgccctat ctcggcggcg cgattaccgt
     2581 ggtgatcgcg gcgatcgtcg cactcacgat cggcgttccc cagcttgttg ccgtcctcgt
     2641 cctctacgcg atcctgatca atatcgagtc ccacattctc gcgccgcgat tcatcggga
//
LOCUS       DASTMD010000500         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_583560, whole genome shotgun sequence.
ACCESSION   DASTMD010000500 DASTMD010000000
VERSION     DASTMD010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583560"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFQ54_07285"
     CDS             complement(<1..642)
                     /locus_tag="VFQ54_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0164834.1"
                     /translation="MELGIHSFAEITEDALTGKVISPEQRLRDLLEEIELADKVGLDV
                     FGVGEHHRPDFAVSSPAVVLAAAAAKTRQIRLTSAVSVLSSDDPVRVFQQFSELDLIS
                     GGRAEIMAGRGSFIESFPLFGYDLSDYDSLFEEKLRLLLAICDNERVTWSGAHRAALV
                     DQPIYPRPVQDRLPIWVAVGGTPKSALRAGILGLPMAVAIIGGGTPGFVPIVDA"
     gene            1042..1233
                     /locus_tag="VFQ54_07290"
                     /pseudo
     CDS             1042..1233
                     /locus_tag="VFQ54_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
     gene            1285..1500
                     /locus_tag="VFQ54_07295"
     CDS             1285..1500
                     /locus_tag="VFQ54_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU0164835.1"
                     /translation="MDPTSLPVASGVVDRFDDLKGFGFIKPDNGGSDVFFHQSALGGA
                     RVYQGDHVEFRLGEGPKGPRAELVEIK"
     gene            1693..2019
                     /locus_tag="VFQ54_07300"
     CDS             1693..2019
                     /locus_tag="VFQ54_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HEU0164836.1"
                     /translation="MSTLKAFGTVAAIEAVSWLALLVAVILKYGPAKDFGSSAVSIIG
                     PIHWVLSVAYVILLYLNYQQRKWTIQKTVIDLVCLVIPFASFWVSKQAFDEDRATSTT
                     AASSAR"
     gene            2069..2857
                     /locus_tag="VFQ54_07305"
     CDS             2069..2857
                     /locus_tag="VFQ54_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEU0164837.1"
                     /translation="MSQRRPNRREERSHARTKAQQQEAIKVRRRRLMMMVSAVLVIAV
                     ATVSIVIAVNNSGSDDDPTAPSPLGTAVFEGIPSSGTTLGNPDAKVTVVEYADYQCPY
                     CANFANQNESQLITDFVKTGQIKYEFEPMPIISQLPLTNPENESVLAAQASLCAVDQG
                     KFWSFHQLLFSKWTGENVGTFTSANLITYASQGGLDVTTFTTCLDSNKYQQTVVDSHD
                     QGIKDGVQGTPTFIINGTVVTLTTSGYDKLKSQIQDAIDGKPIQ"
     gene            2857..>3129
                     /locus_tag="VFQ54_07310"
     CDS             2857..>3129
                     /locus_tag="VFQ54_07310"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEU0164838.1"
                     /translation="MRFPRLSWASFVLAAAGVAVAIYLVYVHYQEHALVCGLGNCEKV
                     QTSKYAELAGIPIAWMGLAMYLVIAVLLIARELRADLLDLTTVGLLG"
BASE COUNT          628 a          974 c          946 g          581 t
ORIGIN      
        1 ggcatcgacg atcggcacga agcctggcgt gccgcccccg atgatcgcga ccgccatcgg
       61 caggccgagg ataccggcgc gcaacgccga cttcggcgtg ccaccgacgg ccacccaaat
      121 cggcaatcga tcctgaactg gtcgcgggta gatcggttga tcgacgagcg cggcacgatg
      181 cgcgccgctc caggtcacgc gctcgttgtc gcagatggcg agcaggagcc gaagcttttc
      241 ttcaaagagc gaatcgtagt cggagaggtc gtagccgaag agcgggaacg actcgatgaa
      301 cgagccgcga cccgccatga tctccgcgcg accgccggag atgagatcga gttcgctgaa
      361 ttgctggaag acgcggaccg gatcgtccga gctgaggacc gacaccgcgc tcgtgagccg
      421 gatctgccgc gtcttcgccg cagcggcggc aagcacgacc gccggggatg agaccgcaaa
      481 atccggccga tgatgctccc cgacgccaaa gacgtcgagc ccgaccttat cggccagctc
      541 gatctcctcg agcagatccc ggaggcgttg ctccggcgag atgaccttgc cagtcagggc
      601 gtcttcggtg atctccgcga aactgtggat tccgagttcc atgttcgtgc actccatgcc
      661 agaagaataa tcgcttacga aaggtaattg tcgtatactt cctcggggat tcccatgagg
      721 aaggagtcga tgcgaaatcg acctgatggg aagacggcag ataatcggag aagcgttcac
      781 attgattggt cgaaatgcat caaggatagt gtgaagagtc cgccctacaa accgcgcccg
      841 ccgggattgg aatcccgcaa caatcgtcgc aacgacgagc tccaggagaa tcgatgatgc
      901 atccctgccg ggagatcgtg cagtgatgga cagttgcgtc tcgcggtggt agtctacatg
      961 cgcaccttca gcatcatagg tacgagtctg aaggctttag gaacggcgcg ctgcgccgaa
     1021 caggttcaac gaggaagcac aatgcccacg ggaacagtca agtggtataa ccctgaaaag
     1081 ggatttgggt tcatcgagcg cgacagcggc ggtgatgatt tgtttgtgca tcgctctgcg
     1141 gtcggttacc agtcccttgg agagggtgac cgcgtcgagt tccgcgaagg tatgggcgca
     1201 aagggaccga gcgccgagga cgttgtcgtc gtcgagacca acccgacgcc gcgtccgcgc
     1261 ggtggcgcgt ctcgcggtcc gtccatcgat ccgaccagcc tcccggttgc ctctggtgtc
     1321 gtcgatcgtt tcgatgacct caagggcttc ggcttcatca agccggacaa tggcggaagc
     1381 gatgtcttct tccaccagtc cgcgctcggc ggcgcccggg tctaccaggg cgatcacgtc
     1441 gaattccgcc tcggcgaggg cccgaagggt ccgcgcgcgg agctcgtcga gatcaagtaa
     1501 cgagcagctg acgagagggg gcggttccat tccggaaccg tccctttttc tgtgtccgca
     1561 ttgcgtcgcc ctaacgcgca tccattccgg catcagactt ttccccgcag gcatcacggg
     1621 gttaacgtgg tattgtccag atcgtgtgct gctgttgtgc gccaccttcc tgttcattcc
     1681 cggagcgacg aaatgtccac actcaaagcg ttcggtaccg tagccgccat cgaggccgtc
     1741 tcctggcttg ccctccttgt cgccgtcatt ctcaagtacg gcccggccaa ggattttggc
     1801 agcagcgcag tcagcatcat cggaccgatc cactgggtgc tctcggtcgc ctacgtgatc
     1861 ctgctctacc tcaactacca gcagcgcaag tggacgatcc agaagaccgt gatcgatctc
     1921 gtttgcctcg tcatcccctt cgccagcttc tgggtctcga agcaggcctt tgacgaggat
     1981 cgcgccacca gcaccaccgc cgccagcagt gcgcggtagt cccgcgccag acgcgtcgcg
     2041 attcaatggg gaatcgggag cctgattcat gagtcaacgt cggccgaaca gacgcgaaga
     2101 gcgatcgcac gcgcgcacga aggcacagca gcaggaggcg atcaaggtac gccggcgccg
     2161 gctcatgatg atggtgagtg cggtgctcgt catcgccgtc gccacggtct cgatcgtgat
     2221 cgccgtcaac aacagcggat cggacgacga tccgaccgcg ccatcgcccc tcgggactgc
     2281 cgtcttcgaa ggcattccct cgagcggcac gacgctcggc aatccggacg cgaaggtcac
     2341 ggtcgtcgag tacgccgact atcaatgccc ttactgcgcc aatttcgcga atcagaacga
     2401 gtcgcagctc atcactgact tcgtcaagac gggtcagatc aagtatgagt tcgagccgat
     2461 gccgatcatc agccagcttc ccctgacgaa tccggagaac gagtctgtcc tcgcggcgca
     2521 ggcgtcactc tgcgcagtcg accagggcaa gttctggtcc ttccaccagc tcctcttttc
     2581 gaagtggacc ggcgagaacg tcgggacatt cacgtcggcg aacctgatca cctacgcctc
     2641 ccagggcggg ctcgacgtga cgaccttcac gacctgtctc gactccaaca aataccagca
     2701 gacggtggtc gacagccatg accaggggat caaggatggg gtccagggca ccccgacctt
     2761 catcatcaac ggaacggtcg tcaccctgac gacgtccggc tatgacaagc tgaagtccca
     2821 gatccaggac gcgatcgacg gaaagccgat ccagtgatgc gattcccgcg gctgagctgg
     2881 gcctcattcg tgctggccgc cgcgggtgtc gccgtagcga tttatctggt ttatgtccac
     2941 taccaggaac atgcgctcgt ctgcggtctc ggaaattgcg agaaggtcca gaccagcaaa
     3001 tatgcggaac tcgcgggaat ccccatcgcc tggatgggac tcgcgatgta tctcgtcatc
     3061 gccgtcctgc tgatcgcgcg cgagttgcgg gccgacctcc tcgacctcac gacggtgggg
     3121 ctcctcggc
//
LOCUS       DASTMD010000501         1988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1743330, whole genome shotgun sequence.
ACCESSION   DASTMD010000501 DASTMD010000000
VERSION     DASTMD010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743330"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VFQ54_07315"
     CDS             <1..1025
                     /locus_tag="VFQ54_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEU0164839.1"
                     /translation="RYGGGSPDFAIEREVLAGYPDLDVELLGAEPDSAADMIALGQTA
                     DAMLLSTRDAVTRKVVSRIPRVKVIGRYGVGLDNVDLDAATEHGIVITHFPQYCTAEV
                     ADHALAFILAANRRIVELDHDLREGAWTRHKADTDAILRGAVPPMRELTIGIIGFGAI
                     GRAVAARLKAFGSRLIAFDPFVSGASFAEAGVESCGFEGLLAEADIVTIHCPLTSETR
                     GLIDAAAFALMKPAGVLVNTARGPIVNQAALTAHLQANPQFRALVDVVEVEPLPLDSP
                     LFALPNVTLTPHSAYYSLRSVEIVQRETLIGALDALSGVRPVTVANPAVLRSFALDAN
                     PRTIDG"
     gene            1274..1906
                     /locus_tag="VFQ54_07320"
     CDS             1274..1906
                     /locus_tag="VFQ54_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0164840.1"
                     /translation="MNEPGPGQENEDERLMIAAQGGDLASFNLLVQRHERAVFSVCLR
                     LLRDVGLAEDAGQDTFIRAWNAIDSFRGGLVRPWLLRIATNRAYDVLRAKARRPAQSL
                     DAELYEIEPEWTSQAQGTEHPETFAARTELSGYLERALASLPDDQRLAIVLADVQGYG
                     YEEVAQIMDVAVGTVKSRISRGRSRLREILRNQEESREHFARYTRLHSSE"
BASE COUNT          336 a          639 c          658 g          355 t
ORIGIN      
        1 atcgctacgg cggcggttcc cccgacttcg cgatcgagcg cgaggtgctc gccggatatc
       61 cggatctcga tgtcgaactg cttggagcgg agccagattc ggccgccgac atgatcgcgc
      121 tcggccagac cgcggacgcg atgctgctca gcacgcgcga cgccgtgaca cgcaaggtcg
      181 tctcccggat cccccgcgtg aaggtgatcg gccgctacgg ggtcgggctc gacaacgtcg
      241 acctcgatgc cgcgaccgag catggaatcg tgatcaccca ctttcctcaa tactgcacgg
      301 cggaggtcgc ggatcacgcg ctcgcattca tcctcgccgc gaaccggcgg atcgtggagc
      361 tcgaccatga cctccgcgag ggggcgtgga cgcgtcacaa ggcggatacc gatgcgatct
      421 tgcgcggcgc ggtgccgccg atgcgggaac tgacgatcgg gatcatcggc ttcggcgcca
      481 tcggtcgcgc cgtcgcggcc cggctgaagg cgttcgggtc gcgcctgatc gccttcgatc
      541 cgttcgtctc cggcgcatcc ttcgccgaag ccggggttga atcgtgcggg ttcgaggggc
      601 tcctcgccga ggcggacatc gtgacgatcc actgcccgct gacatcggag acgcgtggcc
      661 tgatcgatgc cgcggcgttc gcgctgatga agccggctgg cgtgctcgtg aataccgcgc
      721 gtggcccgat cgtcaatcag gcggcgttga ccgcccatct tcaggcgaat ccacaattcc
      781 gcgccctcgt cgatgtcgtt gaagtcgagc cgttgccgct cgactcaccg ctcttcgcct
      841 tgccgaacgt cacattgaca ccgcactcgg cctactactc gcttcgatcg gtcgagatcg
      901 tgcagcgcga gacgctgatc ggggcgctcg atgctctgag cggcgtgcgc ccagtgactg
      961 tcgcgaaccc cgccgtcctg cgttcgttcg cgctggatgc gaaccctcgc accatcgacg
     1021 gatagggacg gggtgactcg accgtgcccc cgtcgcgcgg acccatgatg agcgttcgtt
     1081 cgctccgatg ggatcaccca cgtacaactt tctttcgcca agcgccgcag ggaacctgta
     1141 ggatatacgt atcgacgcgg tgcgcggtcg acgattgctc gacagatgat tctggatgct
     1201 tggttttgcg cagccggtga aacgctttcg ggtggttcgt gcggccgaca caacgtcacc
     1261 atcggacgcg ggagtgaacg aacccggacc ggggcaggag aacgaggatg aacggttgat
     1321 gatcgccgcc cagggcggtg atctggcgtc gttcaatctc ctcgtgcagc ggcatgagcg
     1381 cgcggtgttc agtgtctgcc tccgcctcct gcgcgatgtc ggactcgccg aggacgcggg
     1441 ccaggacacg ttcatccggg cctggaacgc gatcgacagc ttccggggcg gtctcgttcg
     1501 cccgtggttg ctgcgtatcg cgacgaatcg cgcctacgac gtcctgcgtg ccaaggcgag
     1561 gcgacccgcg cagtcgctcg acgccgagct ctatgaaatc gaacccgagt ggacgtcgca
     1621 ggcgcagggg acggaacatc cggagacctt cgccgcgcgt accgagctga gtggatatct
     1681 cgaacgggcg ctggcgtcac ttccggacga tcagcgactc gcgatcgtcc tcgccgatgt
     1741 ccagggatac ggatacgaag aggtcgcgca gatcatggat gtcgcggtgg gcaccgtgaa
     1801 atctcgcatc agccgggggc gttcgcggtt gcgagagatc ctcaggaatc aggaagagtc
     1861 gagggaacac ttcgcccgct acacacgttt acactccagc gagtaatccg gtcgaccggg
     1921 ctggcaccgg gcgactcgcg attttccgta cccggcatca cgcggtgagc ggcaacgacg
     1981 agcagaag
//
LOCUS       DASTMD010000502         1770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3082493, whole genome shotgun sequence.
ACCESSION   DASTMD010000502 DASTMD010000000
VERSION     DASTMD010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3082493"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFQ54_07325"
     CDS             <1..467
                     /locus_tag="VFQ54_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164841.1"
                     /translation="DTINDHAYDDAWIPDMLAGKTMKEVGEDAYTGDLLGDDPLGNFA
                     RYADRAVVSIEQITDLDQIVHCSFGDYSTRDYLYQVANFRGMRAHDVAKVIGVSTELP
                     PDLVSGLYDMILPFAEEWRTIGVFGPKVEVAEDAPLQDKLLGLTGRQPDHRS"
     gene            570..>1770
                     /locus_tag="VFQ54_07330"
     CDS             570..>1770
                     /locus_tag="VFQ54_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0164842.1"
                     /translation="MPRRTPRRFTSDPLINSVDTHQPKPKLTRLDLHGFKSFAQKTAF
                     VFEPGITAIVGPNGSGKSNISDAIRWVLGETSYNALRSKKTEDVIFAGGKGKTPAGLA
                     EVTVTFDNEDGWLPSEFTEVTVTRRAYRGGENQYFINGRRVRLKDVAFLTASLGQSYT
                     VVGQGLVDAALSQRADERRGLFEHAADLTGLRLKVHEAERNLAEADANSERLTDLLTE
                     LEPRLKTMERQAKQAREWQGLRDRLTFLQRGHYHRLLVAATERFGSAERLIAGDAETL
                     DEARARIDALLTERTAASDAIAIARDSLATHQAQLNALAEQVRRVEHERDLVQERRGA
                     LDRRREDMQDTQSGLDEQVARVEAELGALAAELATLETDATAARAAVDALHAAARERR
                     KERTALEQ"
BASE COUNT          336 a          573 c          595 g          266 t
ORIGIN      
        1 gcgacacgat caacgaccac gcctatgacg atgcctggat ccctgacatg ctcgcgggca
       61 agacgatgaa agaggtcggg gaagacgcct acacgggtga tctgcttggc gacgacccgc
      121 tcgggaactt cgcacggtac gcggaccgcg ccgtcgtctc gatcgagcag atcaccgatc
      181 tcgaccagat cgtccactgc tcgttcgggg attattcgac ccgcgactat ctctaccagg
      241 tggcgaactt ccgggggatg cgggcacacg acgtggcgaa ggtcatcggg gtgagcacgg
      301 agttgccacc ggacctggtg agcggtctct acgacatgat cctgcccttc gcggaggagt
      361 ggcggacgat cggggtgttc ggtccgaagg tcgaggtggc cgaagatgcg ccgctccagg
      421 acaagctcct tgggttgacg ggccgccagc cagatcatcg atcctgatcc atcgtcacgt
      481 cccgcgcgtt atcggtgggt gtcagccgtt cggcgtggac gagggatgcg ggcaaggatg
      541 gtaccctttg gtgctgcgta cgatccggaa tgccgcgccg gaccccgaga cgattcacga
      601 gcgatccact gatcaacagc gtcgataccc accagccgaa accgaagctc acccggctcg
      661 atctgcacgg gttcaagagc tttgcccaga aaaccgcgtt cgtcttcgaa cccgggatca
      721 cggcgatcgt gggtccgaac ggctccggga aatcgaacat ctcggatgcc atccgctggg
      781 tgctcgggga gacgagctac aacgcgcttc gctcgaagaa gaccgaggac gtgatcttcg
      841 ctggtggcaa ggggaagact cccgccggcc tggcggaagt gaccgtcacc ttcgacaacg
      901 aggacggctg gctccccagc gagttcaccg aggtcaccgt cacccggcgc gcgtaccggg
      961 gtggcgagaa ccagtacttc atcaacgggc gccgcgtccg cctcaaggat gtcgcgttcc
     1021 tcaccgcctc gcttggccag agctacaccg tggtcgggca gggactcgtc gacgccgcgc
     1081 tcagccagcg cgccgatgag cgccgcgggc tcttcgagca cgcggccgat ctcaccgggc
     1141 ttcggctcaa ggtccacgag gcggaacgca atctcgccga ggccgacgcc aacagcgaac
     1201 gcctgaccga tctcctgacc gaactcgaac cgcgcctgaa gacgatggaa cgccaggcaa
     1261 agcaggcacg cgagtggcag ggattgcggg atcgcctcac gttcctccag cgcggccact
     1321 atcaccggtt gctcgtcgcc gcgaccgagc gcttcgggag cgccgagcga ttgatcgcgg
     1381 gtgatgccga gaccctcgac gaggcgcggg cgcggatcga tgcgcttctc accgaacgta
     1441 ccgccgcgag tgacgcgatc gcgattgccc gcgacagcct cgcaacgcat caggcgcagc
     1501 tgaatgcgct ggccgagcag gtccgccgcg tcgagcacga gcgggatctc gtacaggagc
     1561 ggcgcggcgc actcgatcgc cgtcgcgagg acatgcagga tacgcagtcc ggtctcgacg
     1621 agcaggttgc acgggtcgag gcggaactcg gagcactggc cgccgagctg gcgacgctcg
     1681 aaaccgacgc cacagcggcg cgcgctgcgg tcgatgctct ccacgcagcg gcgagggagc
     1741 gacgcaagga gcggacggcg cttgagcaga
//
LOCUS       DASTMD010000503         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3082577, whole genome shotgun sequence.
ACCESSION   DASTMD010000503 DASTMD010000000
VERSION     DASTMD010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3082577"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /gene="pxpB"
                     /locus_tag="VFQ54_07335"
     CDS             <1..1607
                     /gene="pxpB"
                     /locus_tag="VFQ54_07335"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEU0164843.1"
                     /translation="LSRVMLSEWSALVGSGADDSAAVAREVVIPVVYGGEHGPDLGDV
                     AAHTGLSPDEVVRRHAAGEYAVGALGFVPGFGYLIGLPPELTTPRRATPRVAVPPGSV
                     GIGGVQTGVYSLPTPGGWSLIGRTPLRLFRPESEEPFVIRGGDLVRFDPISLERYDEI
                     AAAETVSSVRPDSSDAKTEATFEVISPGLQTTVQDLGRPGLGRFGVTPGGAADRRALI
                     AANRLVENEDGAAGIEMTLLGPQLRLLRSCRIAIAGADLGAQRNEVPLPFARPIDLAA
                     GDELRFGPVVGGFGARGYLAIAGGIDVPLVMGSRATDLTAGFGGFEGRALRAGDRLAT
                     GECQVAAGENPLSLRRGRNHAATATVQAIRIVRGPQAERFDERAWKTFLGESFAVSSR
                     SDRLGARLDGPPVRPTGGSDVISEGMVTGTIQITTDGQPIVMMPARATIGGYAKIATV
                     ASADLGLLGQLRPGSRVRFVEVTVEEAFRLARNGDDVEEPGMSLQDAVAIVRAFASLP
                     FSALDLELPARDFSLRLRRGHQASPD"
BASE COUNT          249 a          565 c          636 g          337 t
ORIGIN      
        1 tgctctcgcg ggtgatgctg agtgagtggt ctgctctcgt gggctcagga gcggatgatt
       61 ccgctgccgt tgcgcgcgag gtcgtgatcc cggtcgtcta cggtggcgag cacggtcctg
      121 atctcggaga tgtcgccgcg cataccggtc tctccccgga cgaggttgtt cgccggcacg
      181 cggcgggaga atacgccgtg ggcgcgctcg gcttcgtgcc ggggttcggc tacctgatcg
      241 gtctgccgcc ggaattgacg acaccccgtc gtgcgacgcc gcgggtcgcc gttcccccgg
      301 ggtcggtcgg gatcggcggc gtgcagacgg gtgtctactc gctgccgaca ccgggcggct
      361 ggtcgctgat cggccggaca ccgctgcggc tcttccgacc ggagagcgag gaaccgttcg
      421 tgatccgggg cggcgatctc gtgcgcttcg atccgatctc gctcgaacgt tatgacgaga
      481 tcgctgccgc cgagaccgta tcctccgttc gtccggacag ttccgacgcg aagaccgaag
      541 ccacgttcga ggtgatctcg cctggcttgc agacgacggt gcaggatctt gggcgaccgg
      601 gtctcggtcg cttcggcgtc acgcccggcg gcgcggcgga ccgccgggcc ttgatcgcgg
      661 cgaatcgtct ggtggagaac gaggatggtg cggccggcat cgagatgacg ctgctgggac
      721 cgcaactacg actgctgcgg tcctgccgga tcgcgatcgc gggcgccgat ctgggagcgc
      781 agcggaatga ggtcccgctg ccgttcgcgc ggccgatcga tctcgccgct ggtgacgaat
      841 tgcgtttcgg accagtcgtc ggaggctttg gtgcgcgcgg ttacctcgcg atcgctggcg
      901 ggatcgatgt cccgttggtg atgggaagtc gcgcgaccga tctcaccgcc ggcttcggtg
      961 gtttcgaggg tcgggcgttg cgggccgggg atcgattggc gacaggtgag tgccaggtcg
     1021 ccgctggcga gaacccgttg tcgctacgac gaggtcgtaa tcatgcagcg accgcgaccg
     1081 tgcaggcgat ccgcatcgtg cgtggtccgc aggcggagcg gttcgacgag cgcgcctgga
     1141 agaccttcct tggtgagtcg ttcgcggttt cttcccgttc cgatcgcctt ggtgcccggc
     1201 tggacggacc gcccgtacgc ccgacgggag gttccgacgt catctccgag gggatggtga
     1261 ccggtacgat ccagatcacg accgacggtc agccgatcgt gatgatgccg gcgcgcgcaa
     1321 cgatcggcgg ctacgcgaag atcgcgacgg tggccagtgc cgatctcggc ctgctcgggc
     1381 agttgcgacc cgggagccgg gtgcgcttcg tcgaggtgac ggtggaagag gcattccgtc
     1441 tcgctcgcaa cggagatgac gtcgaggagc cggggatgtc gctccaggat gcggtcgcga
     1501 tagtccgcgc cttcgcatcg ctcccatttt ccgcgctcga tctagaactt ccggcgcgtg
     1561 atttctcgct tcgcctccgt cgcggtcatc aggcatcgcc ggactgaacg gacacacgta
     1621 tcggccattg acaatagaga cgtcgccccg gcgtattccg acgcgatcga cacctcgcgt
     1681 tccgatccgc cggggtctgt agggcaagac cttgtgtctt gccgcgtgcc ccacgcccca
     1741 tgcaacgccc ctcgcggcaa cgcgcgtccg ccgagacgtt gatgcat
//
LOCUS       DASTMD010000504         1832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1207664, whole genome shotgun sequence.
ACCESSION   DASTMD010000504 DASTMD010000000
VERSION     DASTMD010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207664"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="VFQ54_07340"
     CDS             complement(<1..1141)
                     /locus_tag="VFQ54_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0164844.1"
                     /translation="MFLTRISISRPVFATMIMVAIMVFGLVGWRSLPIDRYPDIDFPV
                     VAVLTAYSGASPETVENDVTKPIEDELNTLSGIEELTSTSSDGSSLVVVKFKLETDSA
                     LAAQDVRDRIAAVAPSLPDGVKTPLVMRHNPTETPVMSLAVSSASLDPGRLSRLTEDV
                     VVPALTAITGVGSATVVGSVEDQVGVLIDPDRLRAYGLGVDDVVDALRQDNLLQPAGS
                     VTTGTLVRAVQVNSESRTLEQLREVIVARQAGAAVRLGDVASIAASTSDPESLAFRDG
                     QAALAIDVIKVDGANTVAVADDIERAIARLRGQGTIGDARIDVLINSADAIEANYATV
                     RSTLVEGALLAVAIVFVFLNSWRSTVITALTLPISFLGTLAVISLL"
     gene            complement(1145..>1832)
                     /locus_tag="VFQ54_07345"
     CDS             complement(1145..>1832)
                     /locus_tag="VFQ54_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0164845.1"
                     /translation="VKPKSALEDAESSVAMLVASLDASEARVNLARTNLERVKVRAPI
                     SGVVASRHIESGQFVQVGAPLFDVIDLSKVTMEAMVPLNRLVALQPGQTADLWLPQDP
                     EHFFSARVARISPRAEQGSRSAVVYLDVENAEGILRGAMFMTGRIRIREKLDAIALPK
                     DAVTFDGSSASVHLIRDKRIVDSPVTTGAQWQAGNAIEIMDGLVSGDAVLTRPLRGLE
                     PGDLVRIGGN"
BASE COUNT          354 a          602 c          563 g          313 t
ORIGIN      
        1 cgagcaggct gatcaccgcg agggtgccaa gaaaggagat cggcagcgtc agggcggtga
       61 tcaccgtcga gcgccaggaa ttgaggaata cgaacacgat ggcgacggcg agcagcgcgc
      121 cttcgacgag ggtggacctc accgtcgcgt aattggcctc gatggcatcg gccgaattga
      181 tcagcacgtc gatcctcgca tcgccgatcg tgccctggcc ccgcagccgg gcgatggctc
      241 gctcgatgtc gtcggcgacg gcgacggtgt tcgctccatc gaccttgatc acgtcgatcg
      301 ccagcgccgc ctgtccgtcc cggaaggcca ggctttcggg atccgaggtc gaggccgcga
      361 tggacgccac gtctcccagc ctgaccgccg cccccgcctg ccgcgcgacg atcacttcgc
      421 gcagctgttc gagcgtgcgg ctctccgaat tcacctgcac cgcccggacg agggtacccg
      481 tcgtaaccga accggccggc tgcaaaagat tgtcctgccg caacgcgtcg acgacatcgt
      541 cgacaccaag gccgtaggcg cgaagccggt cgggatcgat cagaacgcca acctgatcct
      601 cgacggaacc gacgacggta gcactgccga cacccgttat ggccgtaagt gccggaacga
      661 cgacatcttc ggtgaggcgt gacagccgcc ccggatccaa agaggcgctg cttacggcca
      721 gcgacatcac cggcgtctcg gtcggattgt ggcgcatgac cagcggtgtt ttcacgccgt
      781 ccggcagcga aggtgcgacc gctgcgatcc ggtcacgaac gtcctgggcg gcaagggcac
      841 tgtccgtttc aagcttgaac ttgacgacga cgagagagct tccgtcactg gacgtcgacg
      901 tcaactcctc gattccggaa agagtattca gttcgtcctc gatcggcttg gttacgtcgt
      961 tttccaccgt ctccggcgag gcgcctgaat aggcggtgag gaccgcgacg acgggaaagt
     1021 caatgtccgg ataccggtcg atcggcagcg accgccaccc aacgagcccg aacaccatga
     1081 tcgccaccat gatcatggtc gcaaacaccg gtcgcgatat gctgatgcgt gtcaggaaca
     1141 tcgatcagtt ccccccgatc cggacgagat cgccgggctc gagcccacgc aatggccggg
     1201 tgagcaccgc atcgcccgat acgagtccgt ccatgatctc gatcgcattg ccggcctgcc
     1261 attgcgctcc ggtggtaacc ggcgaatcga cgatgcgctt gtcgcggatc agatgcaccg
     1321 aggccgaact gccgtcgaag gtcacggcat ccttgggaag agcgatggcg tcgagtttct
     1381 cgcggatgcg aatacggccg gtcatgaaca tcgccccgcg cagaattccc tcggcatttt
     1441 cgacgtcgag gtaaacgacg gccgaccgag atccctgttc ggcgcgtgga ctgatccgcg
     1501 ccacccgcgc agaaaaaaaa tgctcggggt cttgcggcaa ccacaggtcc gccgtctgac
     1561 ccggttgcag cgcaaccagc ctgttgagcg gcaccatggc ttccatcgtg accttcgaca
     1621 ggtcaatgac atcgaacagt ggagcgccga cttgcacgaa ttgcccggat tcgatgtgcc
     1681 gactcgcgac cacgccggag atcggcgcgc gcactttcac acgctcgagg ttggtgcgcg
     1741 cgagattgac gcgggcctcc gatgcgtcga ggcttgccac cagcatcgca accgaacttt
     1801 ccgcatcctc cagcgcgctt ttcggcttca ca
//
LOCUS       DASTMD010000505         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1834581, whole genome shotgun sequence.
ACCESSION   DASTMD010000505 DASTMD010000000
VERSION     DASTMD010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834581"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VFQ54_07350"
     CDS             <1..538
                     /locus_tag="VFQ54_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEU0164846.1"
                     /translation="LAAIAATSVYGAPAEAIAAGLASFKGAPGRMEEIATIDGVLYVN
                     NTTATAPVATIAALSVLPGLFNRVHLIAGGSSKRSDLTELGKAIAASGVRVLLLTGSG
                     TDELRAHIATAGGSYEGPFGSMADAMEAAVARVSTGDVVALSPGCASFGMFLNEFDRG
                     RQFDSFVHQIHESVISKS"
     gene            573..>2465
                     /gene="murC"
                     /locus_tag="VFQ54_07355"
     CDS             573..>2465
                     /gene="murC"
                     /locus_tag="VFQ54_07355"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU0164847.1"
                     /translation="MQPTSAVPTTDLHLPPPPANIHFIGIGGIGMSGLARILIAWGYR
                     VSGSDAVASEQTEALAKLGATIVIGHDDPTLASLADMLVTTLRAEQGAAIEVQAARAA
                     GVVQVKRGQLLAMLANARRSIAVAGTHGKSTTTGMLTYALRELGTDPSYAIGATLTAT
                     GLNAAPGNGPFMPVEADEFDRAFLWLRPEVALVTAVSFDHPDIYADQDDYDAAFVEFA
                     QGIVPGGTLVIPADDPGGARVVAALRASGDLPFAVVTFGSSDDADWKIVHGASGGVVT
                     LPDSRALPVNPTVSGRHNVRNTVAAAIALDALGFDPEIALPALVRFDGTGRRFEHKGT
                     LGGIDVVDDYAHHPEEIEATLQAARERFPNRRVIVAFQPHTFSRTKALLDQFAVALSK
                     ADEVVLLEIYPSGENDDLGISSRDIFTRLSTPAHAAARPEDAAAEIATIAKPGDVVLT
                     LGAGDITKVGALLLSNLSESRPVAEEQPARPVRTRAASRAPKPESIAIPERPSLKVQP
                     EAAMSLYTTMRIGGPADFLVRAQEADDIAAAVRWADAEGLPVTVIGGGSNLLVGDRGI
                     RGLVIVVRTPGERAASLLAVEDGGESVELTVGAQAPLSWVGRYCAEHGWAGMDWGVGL
                     PGQIGGA"
BASE COUNT          394 a          794 c          847 g          430 t
ORIGIN      
        1 cctcgcggcg attgccgcga cgtccgtgta tggcgcgccg gcggaggcga tagccgccgg
       61 gctcgcatcg ttcaaggggg cgccgggtcg gatggaggag atcgcgacca tcgacggcgt
      121 gctgtatgtg aacaacacga cggcgacggc gccggtcgcg acgatcgcgg cattgtcggt
      181 tcttcccggc ttgttcaacc gcgtgcatct gatcgccggc ggctcctcga agcgctcgga
      241 tctcacggaa ctgggcaagg cgatcgccgc gtcgggcgtg cgcgtcctgc tcctgacggg
      301 atcgggaacc gatgagctcc gagcgcacat cgcgaccgcc ggaggatcgt acgaggggcc
      361 gttcggatcg atggcggacg cgatggaagc cgcagtcgca cgcgtttcca ccggcgacgt
      421 cgtggcgctc tcgcccgggt gcgcgagctt cgggatgttc ctcaatgagt tcgaccgtgg
      481 gcgccagttc gactccttcg ttcaccagat ccacgaatca gtcatttcga agtcctgatc
      541 gtcaccgatc ggacgtcaca cgcgagaaat cgatgcagcc gaccagcgcc gtcccgacaa
      601 ccgatctgca cctgccaccg ccgcccgcga atatccactt catcgggatc ggcgggatcg
      661 ggatgagcgg tctcgcccgg atcctgatcg cctggggata ccgggtttcc ggttccgatg
      721 ccgtcgcctc cgaacagacc gaagccttgg ccaagctcgg ggcgacgatc gtgatcggcc
      781 acgacgatcc gacccttgcc tcgctggccg acatgctggt gactacgctt cgcgccgagc
      841 agggagccgc gatcgaggtc caggccgctc gcgccgcggg cgtcgttcag gtcaagcggg
      901 gacaactgct ggcgatgctg gcgaacgcgc ggcgctcgat agccgttgcc ggaacccacg
      961 gtaagtcgac gaccaccggg atgctgacgt atgccctgcg ggaactcggg accgatccga
     1021 gctacgcgat cggcgcgacg ctgaccgcga ccggtctcaa cgccgcaccg ggtaacgggc
     1081 cgttcatgcc ggtcgaggcg gacgagttcg atcgcgcgtt cctctggctt cgtcccgagg
     1141 ttgccctcgt taccgcggtg agcttcgacc atcccgacat ctacgccgat caggatgatt
     1201 acgacgcggc gttcgtggag ttcgcacagg ggatcgttcc cgggggcacg ctggtgattc
     1261 cagccgacga tcccggcggt gctcgggttg ttgcagccct tcgcgccagc ggcgaccttc
     1321 ctttcgccgt ggtcaccttc ggctcgagtg atgacgctga ctggaagatc gtccatggcg
     1381 cgagtggtgg ggttgtgacc cttccggaca gccgggcgct gccggtgaac ccgacagtat
     1441 cgggtcgtca taacgtccga aacacggtcg cggcagcgat tgctctcgat gccctcggat
     1501 tcgatcccga gatcgcattg ccggcgctcg tgcgattcga tggaaccggg cggcgcttcg
     1561 aacacaaagg gacgctcggc ggcatcgatg ttgttgatga ctacgctcac catcccgagg
     1621 aaatcgaggc gacgctgcaa gcggcccgcg aacgattccc gaaccggcgt gtgatcgtcg
     1681 ccttccagcc gcacacattc agccggacga aggcgctgct cgaccagttc gcggtggcac
     1741 tgagcaaggc ggacgaggtc gtgctgctcg agatctatcc ctccggcgag aatgacgatc
     1801 tcggtatctc ctcgcgagac atcttcacgc ggctttcgac acccgcccat gccgcggcac
     1861 gacccgagga cgcggcggcg gagatcgcga cgatcgccaa gccgggcgat gtcgtcctca
     1921 cgcttggtgc gggcgacatc acgaaagtgg gcgcactcct gctgtcgaac ctctcggagt
     1981 cccgaccggt tgccgaggaa cagcctgcgc gaccagtgcg gacgcgggcg gcatcgcgag
     2041 cgccgaagcc ggaatcgatc gcgattcccg agcgcccgag cctgaaggtg caacccgagg
     2101 cggcaatgtc gctctatacg acgatgcgga tcggcggtcc cgctgatttc ctcgtccgcg
     2161 cgcaggaggc cgacgatatc gccgccgccg tgcgctgggc cgacgccgag gggcttccgg
     2221 tgaccgtgat tggtggcggg agcaatcttc tcgtgggtga tcgcggtatt cgcggtctcg
     2281 tgatcgtcgt gcggacgccc ggtgaacgcg ccgccagtct gctcgccgtc gaagatggcg
     2341 gcgaatccgt cgagctgact gtcggggcgc aggcgccgct gtcgtgggtc ggtcgttact
     2401 gtgccgagca cggctgggcg gggatggatt ggggcgttgg attgccgggt cagatcggtg
     2461 gcgcg
//
LOCUS       DASTMD010000506         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1743598, whole genome shotgun sequence.
ACCESSION   DASTMD010000506 DASTMD010000000
VERSION     DASTMD010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743598"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VFQ54_07360"
     CDS             <1..1137
                     /locus_tag="VFQ54_07360"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0164848.1"
                     /translation="RVTMLAITNGKVYTALGDIHDTGTVLIDGSKIVAVGPTVEIPEG
                     TEIVDVRGKVVLPGFVESHSHVGIWTEGVGFEGRELNETSDPITPHIRAIDGINPNDP
                     SFRLVREAGITTVLTGPGSANLIGGEWIALKPVGRTVEDMILKEPAGLKMALGENPKT
                     AYGEFKKAPVTRMAQVAMIREQLFKAQKYAESENPERDLRLEPLVRALRREQRTRIHA
                     YTVLDITNGIRFAKEFGLDPVFEHAYEAHTIADILARENIPVSLGPFHLGRMKVEMQA
                     LTFEAPGILARAGVRVAIHMDARGSTALLPVFAGLAVRAGMSEEDAIKAITINSAIIS
                     GVDDRVGSLEPGKDADVIVLSGHPFDWLTKVEKVYVNGQLVHAA"
     gene            1261..>1560
                     /locus_tag="VFQ54_07365"
     CDS             1261..>1560
                     /locus_tag="VFQ54_07365"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEU0164849.1"
                     /translation="MATQTERLDGYRERRNTYFKEHENSPLTPEQKEIFNGLSYFPEN
                     EDLHFTLEFDQLGEDVGEVVTFATVSGSEKQYTRAGRVFFAVDGQDATLSVFRNAT"
BASE COUNT          331 a          439 c          520 g          270 t
ORIGIN      
        1 agagtaacca tgctggcgat tacaaacggg aaggtctata cggcccttgg cgacatccac
       61 gataccggga ccgtgctgat tgacgggtcg aaaatcgtcg ccgtgggacc gaccgtcgag
      121 attcctgaag gcacggagat cgtggacgtc cgcggcaagg tggtcctgcc ggggttcgtg
      181 gaatcgcatt cgcacgtcgg gatctggacg gagggcgtcg gctttgaggg ccgcgagctc
      241 aacgagacgt cggatccgat cacgccgcac atacgggcaa ttgacgggat caacccgaac
      301 gacccttcgt ttcgactcgt tcgtgaagcg ggtatcacca ccgtcctgac gggaccaggg
      361 agcgcaaatc tcatcggcgg cgaatggatc gcgctcaaac cggtgggacg gaccgttgaa
      421 gacatgatcc tgaaggaacc ggcgggtctg aagatggccc tcggggagaa cccgaagacc
      481 gcctatggtg aattcaagaa ggcgccggtc acgcggatgg cgcaggtggc aatgattcga
      541 gagcaactgt tcaaggcgca gaagtacgcc gagagcgaga atccggagcg agatctccgt
      601 ctcgaaccgc tcgtccgcgc gttacggcgg gaacagcgga cgcgcattca cgcctacacc
      661 gtgctggaca tcaccaacgg catacgcttc gccaaggagt tcgggctcga cccggtcttc
      721 gagcatgcct atgaagctca tacgattgcc gacatcctgg cgagggagaa cattccggta
      781 agtctcggtc cgttccacct cggccggatg aaggtggaga tgcaggcatt gaccttcgag
      841 gcgcccggaa tcctcgcccg cgccggggtg agagtggcga ttcacatgga cgcgcgggga
      901 agtaccgccc tgctgccggt ctttgccgga ctggccgtgc gcgcggggat gagcgaagag
      961 gatgcgatca aggcgatcac catcaactcg gcgatcatca gcggcgtcga tgatcgtgtc
     1021 ggcagcctcg agccgggcaa ggacgcggac gtgatcgtcc tctccggtca tcccttcgac
     1081 tggctgacca aggtcgaaaa ggtctacgtg aacgggcagc tggtgcatgc ggcgtaggca
     1141 tgaaagtctc agccacgccg ggtgatcgtc ctgttcgccg aagcgccgtc gtcatatgga
     1201 agaacgtacc gtccgggcgc tccccagagg gggtgccggg tcaagcgagg gaagatgcaa
     1261 atggcaacgc agaccgaacg tcttgacggg tatcgcgagc gcaggaacac ctatttcaag
     1321 gagcatgaga actctccgct gactcccgag cagaaggaga tcttcaacgg tctctcgtac
     1381 ttccccgaga atgaggacct ccacttcaca ttggaattcg atcagctcgg cgaggatgtc
     1441 ggcgaggtcg tgacgtttgc gacggtgagc gggagcgaga agcagtacac ccgcgccgga
     1501 cgcgtgtttt tcgcggttga tgggcaggat gcgacgctga gtgtcttccg gaacgcgacg
//
LOCUS       DASTMD010000507         3970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2457711, whole genome shotgun sequence.
ACCESSION   DASTMD010000507 DASTMD010000000
VERSION     DASTMD010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2457711"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..2647
                     /locus_tag="VFQ54_07370"
     CDS             338..2647
                     /locus_tag="VFQ54_07370"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU0164850.1"
                     /translation="MTNIVSLPQRKSLLDILTAHSSDDSESPNGYAPQFDAPYEPMVV
                     EALRHLDAALAKDALVTDVEFVARAFKLAFTAHDGVRRKSGEPYIVHPVETTAILAMM
                     NLDAETLAGGLLHDVVEDTEVTSEEIEEQFGVRVSRLVDGVTKLGQIPWTPESEDGRT
                     LVAREKEHQAESLRKMFLAMVDDIGVVLIKLADRLHNMRTLGAMPPHKQVRIAQQTME
                     IYAPLANRLGIWQVKSELEDLAFQYLRPLDFQQITMELSKRGADSAAYISRVKSDLQE
                     LLQQAGIQADMSGREKHAYSIYRKMNRKGRSFEEIYDVIGIRILVDTVQECYSVLGIV
                     HGRWHPVPGEIDDYIATPKESMYQSLHTAVIGPDGHPVEIQIRTWEMHRVAEYGIAAH
                     WRYKEGGKSDARLEAKITWLRQLMDWRDEVADAEEFVESLKSDVFQDMVYCFTPAGDI
                     IELPNGATPVDFAYRIHTQVGHHCVGATVGGQMVPLDYKLQNGQVVKIRTSKTVNGPR
                     RDWLQASSGYVKTASAREKIRQWFRRQERDENISQGKEILERELKRLGLPEMKLDEVL
                     HRFPRFTKLDDFLAAIGYGGVSAQQIAYKLDEKPQEVFPVSTSVKAPTTPARVEVMGA
                     GGMLTIQANCCRPVPGDPIIGYVTRGRGVVYHRRDCVNVTNLPDPERLVAVSWGKEAH
                     ETYPVPVRLYALDRVGLLKDVSTLLSDERVNILSVLTQTHDDRTVTLLLTVEVESVSQ
                     LSDIMRRLEGVRDVTGVERDVPHSQARAS"
     gene            2644..3606
                     /locus_tag="VFQ54_07375"
     CDS             2644..3606
                     /locus_tag="VFQ54_07375"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEU0164851.1"
                     /translation="MSEQVRPVISIGECLVDLIVGPGHDLSDASTLNVREGGAPMNFA
                     VALARLGVASAMCAVVGDDPFGDRLIALLDREGVDVSGIRKTADAETTIAYAWRDERG
                     DGRFRLVRMADRLLSGEDIARLPIGGAAAIQICSISLSAQPSRGATEDAIEVANRLGI
                     PVIFDVNVRATLWLDRASLGEACLPRMARATVLKLSLDDADALWGFTSIDDVQRVLDA
                     FDAPIVALTDGARATWIRRGGDWSRHDVFPVEAIEPTGAGDAFGAALVSRLLLRDPAD
                     WTNLGDAEIRFAMAAGAIATTKPGALPALPTVPEIEAFVVARST"
BASE COUNT          722 a         1250 c         1287 g          711 t
ORIGIN      
        1 gaggcgacca cgtgggtcgc cgggacgggt ggcgcaagga gcggcgacaa ggggcgtgag
       61 ggcgggtttt gggagcgcga cttgaccggt gtgacataat ctatgcaata catgcatgaa
      121 cccaacgatg cgcaggctca ctgagactca ttacgataca aagttcacaa atcattgacg
      181 cggaatttgt tgcatgccta cacttgccgt cagcaacggt gctatcggcg acattggctc
      241 cgcgcctgct gagcgttcga gagctctccc gggacacagg tttcccttcg gaaacgcctc
      301 acaggataca cgcggatctc gcggagcgac gccacggatg acgaatatcg tttcccttcc
      361 tcaacgcaag tcgttgctgg atatccttac cgctcattcc agcgacgatt cagagagccc
      421 gaatggctat gcgcctcaat tcgacgcgcc ctacgagccc atggtcgtcg aagcgttgcg
      481 tcatctcgat gccgcgcttg ccaaggatgc cctcgtcaca gacgtcgagt tcgtcgctcg
      541 tgccttcaag ctggccttca ccgcccatga tggcgtgcgt cgcaagtccg gcgagcccta
      601 catcgttcat cccgtcgaga cgaccgccat cctcgcgatg atgaatctgg acgcggagac
      661 gctcgccggc ggcctcctcc acgacgtcgt cgaggatacc gaggtcacca gcgaggagat
      721 cgaggaacaa ttcggcgtcc gcgtctcccg tctcgtcgac ggcgtgacca agctcgggca
      781 gattccctgg acgcccgaat ccgaggatgg ccggacactg gtcgcccggg agaaggagca
      841 tcaggcggaa agcctgcgca agatgttcct cgcgatggtc gacgatatcg gtgtcgtgct
      901 gatcaagctc gccgaccgcc tgcacaacat gcgtacgctg ggcgccatgc cgccccacaa
      961 gcaggtacgc atcgcccagc aaaccatgga gatctacgct ccgctcgcga atcgactggg
     1021 aatctggcag gtcaagtccg aactcgagga cttggccttt caatatctca ggcccctcga
     1081 tttccagcag atcacgatgg agctctcgaa gcggggcgcc gacagcgcgg cctatatcag
     1141 tcgggtgaaa tcggatctcc aggaactcct gcagcaggca gggatccagg ccgacatgag
     1201 cggccgcgag aagcacgcct actcgatcta tcgcaagatg aatcggaagg gacgctcctt
     1261 cgaggaaatc tacgatgtca tcggcatccg gattctcgtc gataccgtcc aggagtgcta
     1321 cagcgtcctc ggcatcgtgc atggccgctg gcatccggtg cccggagaga tcgacgacta
     1381 catcgcgacg ccgaaggaga gcatgtacca gagcctccat acggctgtga tcggacccga
     1441 cggtcatccc gtcgagatcc agatccggac ctgggagatg catcgcgtcg ccgagtacgg
     1501 catcgcggcg cactggcgct acaaggaggg cggcaagtcc gacgcccggc tcgaggccaa
     1561 gatcacctgg ctgcgccagc tgatggactg gcgggacgag gtagccgacg ccgaggaatt
     1621 cgtcgaatcc ctcaagtccg atgtcttcca ggacatggtc tactgcttta ccccggctgg
     1681 agacatcatc gaactgccga acggtgccac cccggtcgat ttcgcgtatc gcatccatac
     1741 gcaagtcggc catcattgtg tcggggcgac ggtcggcggg cagatggtgc cgctcgacta
     1801 caagctgcag aacggccagg tcgtcaagat ccggaccagc aagacggtca acggaccgcg
     1861 gcgcgactgg ctgcaggcga gttcgggcta tgtgaagacg gcctccgccc gtgagaagat
     1921 ccggcagtgg ttccgtcgcc aggagcgcga cgagaatatc tcgcagggca aggagatcct
     1981 cgagcgcgag ctgaagcggc ttggcctccc cgagatgaag ctcgacgagg tcctgcatcg
     2041 cttcccgcgc ttcacgaagc tggacgattt cctcgccgcg atcgggtatg gcggcgtttc
     2101 ggcgcagcag atcgcctaca agctcgacga gaagccgcag gaagtcttcc cggtctcgac
     2161 atccgtcaag gcgccgacga cgccggcccg ggtcgaggtg atgggcgccg gcggcatgct
     2221 gacgatccag gcgaattgct gtcgcccggt accgggcgat ccgatcatcg gctacgtgac
     2281 gcgcggtcgc ggggtggtct accaccgcag ggactgcgtg aatgtgacga atcttcccga
     2341 tcccgagcgg cttgtcgccg tttcctgggg caaggaagct catgagacct accccgttcc
     2401 ggtgcgactt tatgcactcg atcgcgtcgg actcctgaag gacgtcagca cgctgctctc
     2461 cgacgagcgc gtgaatatcc tctccgtcct cacccagacc cacgacgacc gcacggtgac
     2521 gttgctcctg accgtcgagg tggagagcgt ttcccagctc tccgacatca tgcgacgact
     2581 cgaaggcgtc cgcgatgtga cgggcgtcga gcgcgacgtg ccgcattccc aggcaagagc
     2641 gagttgagcg agcaggttcg tccggtcatc agcatcgggg agtgcctggt cgacctgatc
     2701 gtcgggcccg ggcacgatct ctccgatgcc tcgacgctga acgtccgtga gggcggtgcg
     2761 ccgatgaact tcgcggtcgc cctcgctcgc cttggtgtgg cgagcgcgat gtgcgccgtc
     2821 gtcggggacg atccgtttgg cgatcgcctc atcgcgctgc tcgatcgcga gggcgtcgat
     2881 gtctccggaa tccggaagac cgcggacgcc gagacgacga tcgcctatgc gtggcgggat
     2941 gagcgtggcg atggccgctt ccgtctcgtg cggatggccg accggttgct ctccggtgag
     3001 gacatcgcgc ggctgccgat cgggggagcg gcagcgattc agatctgctc gatctccctc
     3061 tccgctcagc cgtctcgtgg cgcgaccgag gatgcgatcg aagttgccaa tcgcctcggc
     3121 attccggtga tcttcgatgt caacgtgcgc gcgacattgt ggctggatcg cgcgtcactc
     3181 ggggaagcct gtctcccacg catggcgcgg gcgacggtgc tcaagctcag tctcgatgac
     3241 gcggacgcgc tatggggatt cacgagtatc gatgacgttc agcgggtcct cgatgcattc
     3301 gatgcgccga tcgttgcgct gaccgacggg gcgcgggcga cgtggatccg tcgtggtggg
     3361 gattggagtc gccacgacgt cttcccggtc gaggcgatcg agccgaccgg cgcgggcgat
     3421 gccttcggcg cggcgctcgt ctcgcgcctc ctcctgcgcg atcccgcgga ttggacgaac
     3481 ctcggcgacg cggagatccg attcgcgatg gccgccggtg cgatcgcgac gacgaagccg
     3541 ggtgcgctcc ccgccctgcc gacggtgccc gagatcgagg cgttcgtcgt cgcccgctcg
     3601 acctagggcc agacccgcaa cggtacccgg gttggggcac gacgtgctgc tcccgcgccc
     3661 gctctgcggc ggggtatgaa acccaacgtt gtcgcctaaa ccgcgcgggg cggacatgaa
     3721 gaccaacgtt tgataccagg tgccttttta aaagatggtc tggcggtgcc cagccggagc
     3781 ggccgcaacg cctgcggaga cgggaatggc gaacctgtcg ttccaaccgc gtcttgaggc
     3841 gggacattga agtcccgccg tacttacgac cgccgtatca acgatcctgg ttggagatcg
     3901 ccgttctcgg gacaccgtcg gcacgaccgg gtttgtgggg cgggagctta tctctcgccg
     3961 cgacgggagc
//
LOCUS       DASTMD010000508         5514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2725735, whole genome shotgun sequence.
ACCESSION   DASTMD010000508 DASTMD010000000
VERSION     DASTMD010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2725735"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFQ54_07380"
     CDS             complement(<1..693)
                     /locus_tag="VFQ54_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164852.1"
                     /translation="MEQRGRFAQLSDDLKSGKIDRRQFMERATALGVGFAAALFAANA
                     ASVAAAGGSRNGYALYPNQDGTPAPSPVAGGAAAPAVGTEGKTRGQEGELKLIQWQAP
                     TLASPQIATGDKDFLAGQLVLEPLMHYLPSGTIIPNLVTDVPSVENGLLAKDLSTVTF
                     KLIQNVTWSDGEPFTSTDVKFTWQWVTNTTRTSNLSVNSSVWAVVKDIATPDPLTAVV
                     TYAKPSAAWFDPF"
     gene            complement(1680..2867)
                     /locus_tag="VFQ54_07385"
     CDS             complement(1680..2867)
                     /locus_tag="VFQ54_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164853.1"
                     /translation="MTYKPGPSPDAIVASSKRHDRTPEDGLRRGTRRSWRHRVLAATM
                     TVSLVGSAVAGTAGLAGAQDGTGAATPTPPEACAVSPATWSKAIADAPTATPAPSPTV
                     APTTAASPIIDGTPGASPAATPQASPVVPVVITPTTAPTGTPPPTAADLLQHDLTATS
                     AAIVGCLSEGKAPTLVKLTGDDFRGQLIGSDQAIGADDFLSLADTLPVLPYHLLTVTD
                     ATVDEDSARSDNITATAVVTYTVGKQIRAGTWTFHWTKVDGKAAWVLESEAPRAPAAP
                     DGAAKANVTIKDNKFQLATGSFPDADVSFTIKNEDAADHEFFVIQLGGSVKADSLLTN
                     TGPGLPKGVTFIGQVTVLAGDSSQIVLTGLAPGTYTVVDLLPDDEGIPHLSDGMEATF
                     TIK"
     gene            3159..4046
                     /gene="dapF"
                     /locus_tag="VFQ54_07390"
     CDS             3159..4046
                     /gene="dapF"
                     /locus_tag="VFQ54_07390"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU0164854.1"
                     /translation="MQDAGLPDRAIPFAKMNGSGNDFVVIDNRNGVLAGSALPRFTRQ
                     VCRRGLGLGADGVVTIEPVAAGPGEPDFRWRYLNADGSDGEMCGNGAMCGARFAVMNG
                     IAPRRCVFQTPSGLVRAEIDPGSSRVTLAMVDSGIVQDPVTLELAGRVLTLHPVRVGV
                     PHVVAFVDDADGFAGAADFAELGRAVRFHDRFAPAGTNVNIVSRIDHRALRMRTYERG
                     VEAETLACGTGSVATAIVAHRVLGMPLPLTIRTSSGRPLDVGFDWDEVRQVATEIRLT
                     GEARVVATGELDPEGWLDA"
     gene            4285..4695
                     /locus_tag="VFQ54_07395"
     CDS             4285..4695
                     /locus_tag="VFQ54_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164855.1"
                     /translation="MIVFWKSRREPVGPLAHIPAAETTSSPQSAGTIDRARMTQPLTL
                     HQQQRLLDAAREIDRAVNVFTVNELRKVLEDIDDESSYWREGYRLFACHDLGMRGGED
                     PRDERLVREEWEGLGPDSQREYCEAARFFRQDLS"
     gene            5035..>5514
                     /locus_tag="VFQ54_07400"
     CDS             5035..>5514
                     /locus_tag="VFQ54_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164856.1"
                     /translation="MTEATINPLTSESADGEDAATTSVESGSTPPPVVETWEEIAAPT
                     AAEATSTHEDPFTTPYPVVGSPEVSPDGRRIAYLLTDGDGATGLWLSPTDGGDAVKLE
                     IPFVPIEDVDPDTGRIQRGPQWSPDGLTIAVAGTHPEAKDRTAIWLVPVQPLPAPVVA
                     "
BASE COUNT          941 a         1652 c         1908 g         1013 t
ORIGIN      
        1 gaacgggtcg aaccaggcgg cggacggctt ggcgtaggtc acgacggccg tcagtggatc
       61 tggcgtcgcg atgtccttga ccacggccca gaccgaggaa ttgacggaga ggttggacgt
      121 gcgggtggta ttcgtcaccc actgccacgt gaacttcacg tcggtcgagg tgaacggttc
      181 gccatcactc caggtgacgt tctggatgag cttgaaggta acggtgctga gatccttggc
      241 gagcaaaccg ttctcgacac tcgggacatc ggtgacgaga ttcgggatga tcgtgccgga
      301 gggcaggtag tgcatgagcg gctcgagcac gagctggccg gcgaggaagt ccttgtcacc
      361 ggtggcgatc tgcggcgagg cgagggttgg tgcctgccac tggatcagct tgagctcgcc
      421 ctcctgaccg cgggtcttgc cttcggtgcc gactgccggg gctgccgcgc cgccggcgac
      481 gggactgggg gccggggtgc catcctgatt cgggtaaagc gcatagccgt tccgggaacc
      541 gccggctgct gccaccgagg cagcgtttgc ggcaaagagt gccgcggcga agccgacgcc
      601 gagggcggta gcacgctcca tgaactgacg acgatcgatc ttgccactct tgagatcatc
      661 gctcagttga gcgaaacgtc cgcgttgctc catcgagaac ctccaacaat gaaccatgac
      721 cgtcggttgg acgattgatg ttgggcgaaa gcgataggtt caaatccccg aaactatcga
      781 atccgtcgtt ggcaaaccac atcatcgata cgatgtggac ctcgcagtag gaccccggcg
      841 caggcaacgc gcatcccctg aaaacagaga gaagattgca cgaggtcagc ggaattgcgg
      901 gctgcgtcgc ccacctatcc gtactcaagc aatctgctcg ttgcgcacac cttccgtgcg
      961 gaccgagctc cacgatccgg caaggtgagt gttgcccctt atggggggtg gcatcaaccc
     1021 acggtgtcgt gcaaatggct caaaaggcgg gtcaatttgc ttaggagtgg cgcggatatg
     1081 agtgaaacga ggaattcgtg tttgggtcgg tacttggatt caggcgggga atgatgaaat
     1141 cagacaacgg ttttgtgcat caagtacgaa cgtgatctca ttcctgtaca aagcgcgcgt
     1201 gagattcgca cattttgcag gaaaggggcg cggtccggca aagccgcatg tttgcaggat
     1261 ccatgtgatc gggaagatgg cgggggccca tgccattcgg ctcccgttcc gggccttccg
     1321 gcgaacgcgg ggcgtcagga gcatgcactg accccccttc tgtcgtccgg gtgggatggt
     1381 ctggtcagga ccatcctccc gtcgctaccc gtgtgcggct ccgaaccgct ggccgtatcg
     1441 tgccggaacg gcgacaagcg ccgtgatgac gggattggag ggcaatgctg tcgcgtggcg
     1501 tccgcctgcg gcggcggcgg gcgagcctcg tgctcgcccc tacgaaccgg gggatttctc
     1561 gaccggcgtg aaatgacgaa tgtgtatgcg gcagcgttgg gaatggagaa cgctcatcgc
     1621 aaggcgggac acggaggttc caccccgagc ccgccctggt cctggtcgag aggccgagcc
     1681 tacttgatcg tgaacgttgc ctccatgccg tccgagaggt gggggattcc ttcgtcgtcc
     1741 ggcaggagat cgaccacggt gtaggttccc ggcgcgagcc cggtcaggac gatctgcgaa
     1801 ctgtcgcctg cgaggaccgt gacctggccg atgaacgtca cgcctttcgg gagtccggga
     1861 ccggtgttcg tgagcaggga atcggccttc acggacccgc caagctggat cacgaagaac
     1921 tcgtgatcgg ccgcgtcctc gttcttgatc gtgaagctga catccgcgtc cgggaaggaa
     1981 ccggtcgcga gctggaactt gttgtccttg atcgtcacgt tggccttggc ggcgccatcc
     2041 ggtgccgctg gcgcgcgtgg cgcctccgat tccaggaccc aggccgcctt gccgtcgacc
     2101 ttcgtccagt ggaatgtcca ggttccggcc cggatctgct tgccgaccgt gtaggtgacg
     2161 acggccgtcg cggtgatatt gtccgagcgt gcgctgtcct catccaccgt cgcatcggtg
     2221 accgtgagca gatggtatgg caggaccggc aacgtatcgg cgaggctcag gaaatcgtcg
     2281 gcgccgatcg cctggtcgct gccgatcaac tgaccgcgga agtcatcgcc ggtcagcttg
     2341 acgagggtcg gggccttgcc ttcgctgaga cagccgacga tcgccgccga cgtcgcggtg
     2401 aggtcatgct gaaggaggtc cgccgcggtt ggcggtgggg tcccggtcgg cgccgtcgtc
     2461 ggagtgatga cgaccgggac gaccggactc gcctgcgggg tggcggctgg cgaggcgccg
     2521 ggcgtgccgt cgatgatcgg ggacgccgca gtcgtcggtg caaccgtcgg tgacggcgcc
     2581 ggggtcgcgg tcggggcgtc ggcgatcgcc ttgctccacg tcgcgggaga aacggcgcag
     2641 gcttcgggtg gcgtcggcgt ggcagcgccg gttccgtcct gggcgccggc gaggcccgcg
     2701 gtgcccgcaa ctgcgctgcc gacgagggag acggtcatcg tggctgcgag gacgcggtgt
     2761 cgccaggaac gcctggtccc ccgacggagg ccgtcctccg gggtgcgatc gtgacgcttc
     2821 gacgaggcga cgatggcatc aggagaaggt cccggtttgt aggtcacgaa aacatccttc
     2881 catgagactg gctgtgattg tggccgcgcg gttggcgatc ccgcgacgag gggattggcg
     2941 cgtatcggtg cacgcctacc atcggctgcg cacggtgacg agcaggtgtc cgtacacttt
     3001 cgaaaacgat tgtagccgat gatcggtgtt gtctatcggc ggcggaattc gatttcgtgt
     3061 aggcaccctc gattgtgcgt gaggatcgga aacgtatcgt tgcggtgacg tgcgaagatg
     3121 aggcggttgg ttcgctgatg cgtgatcggg atcgtgagat gcaggacgcg ggattgcctg
     3181 accgcgccat tccctttgca aagatgaatg gcagcgggaa cgatttcgtc gtgatcgaca
     3241 atcgaaacgg ggtcctcgcc gggagcgcgc ttcctcgatt cacccgccag gtctgccggc
     3301 gcgggcttgg cctcggcgcg gacggcgtcg tgacgatcga gccggtcgca gcagggccgg
     3361 gtgagccgga tttccgctgg cgatacctca atgccgacgg ctccgacggc gagatgtgtg
     3421 gaaacggcgc gatgtgcggg gcacgattcg cggtgatgaa cggaatcgcg ccgcggagat
     3481 gcgtcttcca gacgccgagc ggtctcgtcc gtgccgagat cgatcccggc agctcgcggg
     3541 tgacgctggc gatggtcgat tcggggatcg tgcaggatcc ggtgacgctc gagcttgccg
     3601 gtcgcgtgct gacgctgcac cccgtccgcg ttggcgtgcc gcacgtcgtg gcgttcgtcg
     3661 acgatgctga cggcttcgcc ggcgcggcgg acttcgcgga actcgggcgg gcagtccgct
     3721 tccacgatcg cttcgcgccc gcgggcacga acgtcaatat cgtgagccga atcgaccatc
     3781 gcgcgttgcg gatgcggacc tacgagcgag gcgtcgaggc ggagacactc gcctgcggga
     3841 ccggttccgt ggcgaccgcg atcgtcgcgc accgggtgct tggaatgccg ctcccgttga
     3901 cgatccggac gagcagcgga cgcccgctgg atgtcggctt cgattgggat gaagtgcggc
     3961 aggtcgcgac ggagatccgc ctgaccggtg aggcccgcgt cgtggcgacc ggggaactcg
     4021 atcccgaggg gtggctcgac gcgtgatgcg gcgccgtgag gctccgctcg gtgggaacga
     4081 ttcgctatcc ggtatcgttg cttgacccct gtacgtacga tggtactggg ccatgaggcc
     4141 tatgcttccc atccgataat ccatgtagta ctatcggcat caagcctcgc gagagcgctc
     4201 tcatgcattg aggtccaggg ctgaacggac ctctggtcgt ggtgcgcccg gtcgcccgtt
     4261 tcctcattct ctatggaacg caccatgatc gtcttctgga aatcgcgtcg ggaaccagtg
     4321 ggtcccctcg cacacattcc cgcggcggag acgacgtcat cgccgcaatc tgccgggacg
     4381 atcgatcggg cccggatgac gcaaccgttg acgctccacc agcagcagcg gttgctcgat
     4441 gccgcccgcg agatcgatcg tgcggtcaac gtcttcacgg tcaacgaact ccgcaaggtt
     4501 ctcgaggata tcgacgacga aagctcgtat tggcgggagg gctatcgcct gttcgcctgt
     4561 cacgatctcg ggatgcgtgg cggcgaggat ccgcgggacg agcgactcgt gcgggaggag
     4621 tgggaagggc ttggcccgga ttcccagcgc gagtactgcg aggcagccag gttcttccgg
     4681 caggaccttt cctgacccca tcacccgcga atcgatcgca atgtcacgaa accggccgta
     4741 aacgcggtcg gtttcgttca tatctggcat gcaaaccagc atcgggctac catgtcccca
     4801 acacggcgca gacgatctgc ccgatggggt tcgtgtgcgg tcgctccggt ggcctatcca
     4861 gacgcaatga ttgcagagcc ggctcacacg cagcagccct ggctgcggcg acgatccggc
     4921 ttggggtgtt gcgcgcggca tagtcaagct gtcatgcggg tgtgtccatc atccggtgac
     4981 gggaatgcgt tgcgttctga tcgcgcgcga ccaccaggag tgagagacga ttccatgacg
     5041 gaagccacaa taaacccgct cacatccgaa tccgccgatg gggaagatgc cgccacgaca
     5101 tccgtggaat ccgggagcac cccccctcct gtcgtggaaa cctgggagga aatcgcggcg
     5161 cccacggcag ccgaggcaac ctccacacat gaggatccct tcacgactcc atatccggtc
     5221 gtcggctcgc ccgaggtctc gcccgacggg cgacggatcg cctatctcct taccgatggc
     5281 gacggcgcga ccggtctctg gctgagtcca accgacggcg gcgacgcggt gaagctcgag
     5341 attcccttcg tcccgatcga ggatgtcgat cccgataccg ggcgcatcca gcgcggcccg
     5401 cagtggtcgc cggacggtct gacgattgcg gtcgcgggca cacatccgga ggcgaaggac
     5461 cgcacggcga tctggctcgt gccggtgcag ccacttcccg cgccggtcgt tgcc
//
LOCUS       DASTMD010000509         7721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2638457, whole genome shotgun sequence.
ACCESSION   DASTMD010000509 DASTMD010000000
VERSION     DASTMD010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2638457"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VFQ54_07405"
     CDS             <1..873
                     /locus_tag="VFQ54_07405"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU0164857.1"
                     /translation="RAIPGSMSEEEHDGLEEDLRPHKYEADDEVIGADEREMIDGILS
                     LEELTARDIMVPRLDIIAVDRFVSSQELIETITKAGHSRIPVYQESIDRVLGVLYAKD
                     LLPFVIGSTSRIPLLDLIRPAFVVPESKRLNELFTELKRARIHIAIVADEYGGTAGLV
                     TIEDILEEIVGEIQDEYDFEEEQFQLLAPDVLLADGRLPIEDVEDALGVDFEEDDDFG
                     TLGGFVHKHLGRLPIKGDVFEAEGVRVEILGVERHRVRKLRITKLAPPEEEVGSGRRG
                     GRGNDRDEPDESTE"
     gene            941..1714
                     /locus_tag="VFQ54_07410"
     CDS             941..1714
                     /locus_tag="VFQ54_07410"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU0164858.1"
                     /translation="MNDVRLGRPRLHLPRTGSTMDDLLALARVGAPEGTVVTTDFQDA
                     GQGRAGRVWAAPPGSALLMSTLLKPGRPHAELTPLSLLVALAIATSIRDGFGLDARVK
                     WPNDVFVGRRKAVGVLLRTHAIRGESQPVVVVGTGINVNVPAASLPETGTSIAVELGR
                     EVDRDVVRDRVLAELGDVYHRFLRGDIEADWDRLNQLLLYRDEPVSIQDGDRLLTGTL
                     AGLDHRGGLLLMQPDATMRTVVSGDLTRGPQPIGDRSAS"
     gene            1799..2998
                     /locus_tag="VFQ54_07415"
     CDS             1799..2998
                     /locus_tag="VFQ54_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0164859.1"
                     /translation="MNFELTDDQIQVRDMVREFARREVAPHIQQWDAKGEYHREIIEK
                     MGEAGFLGLPIPEEYGGLGLDYVSLALACDELEYVDTSLRVVLSVHVGLNSLALLQWG
                     TEEQKQRWLIPQATGEKIATFGLTEPGAGSDAGSIATTAVRDGDSYILNGNKMWISLA
                     DVADHFLIFASVDRSLKHRGLVAFVVERGMVGLTTGTIKGKLGVRAGNTGEIAFNNVR
                     VPIENRIGEEGEGFKIAMSCIDQGRFTVAAGAVGLTRASLDASVAYANQRETFGQKIG
                     RHQLVQQMISNMVLGHETSKLLVMQAAELKNRGIRNTKETSLAKWHACDVALKSADDA
                     VQIHGSYGFSNEYPVERFLRNARGAVIYEGTREVHTILQAEYALGYREDKPLSHPQLP
                     ASGFEEE"
     gene            3001..4182
                     /locus_tag="VFQ54_07420"
     CDS             3001..4182
                     /locus_tag="VFQ54_07420"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU0164860.1"
                     /translation="MGNTVILGGARTPFGKLSGSLGSKSAVELGTIALNAAIDRSGVE
                     RRSIEHVTFGQVLQGGAGQNPARQVSFAAGLDRTVTSETINHVCGSGIRAVGLADNAI
                     RVGDQKVIATGGMESMTNAPYILRGARGGYRMGDGKIEDMMVLDGLFCAIEHESMGAI
                     CGVVGDEHHITREEQDAWALRSHLKALDAIDSGWMARQIVPVEVAGRRETTIVDTDEA
                     PRRDTSLEALARLKPAFAAAGTITAGNAPGVNDGATATIIADAEWASAEGLPVQAHIL
                     GHAQAAWDAPYLAYTPAMATELALKKAGLTVADVDLFEINEAFASVTIISTQRLGIDP
                     EKVNIHGGAIALGHPLAASGGRLLLTLIESLKDIGGGIGVAAICTGGGQGDAIVIRVD
                     G"
     gene            4179..4748
                     /locus_tag="VFQ54_07425"
     CDS             4179..4748
                     /locus_tag="VFQ54_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEU0164861.1"
                     /translation="MSERQQRLAALAERHPGAIILPFDGHWPVIHETAFIAPGAVVIG
                     RVTLGEETSVWFNTTVRGDIAPISIGDRSNVQDGTVVHVNEDAPVVVGNDVTIGHSAL
                     IHGTVIGDGTMIGMGAIVLSYSKIGSGVVIAAGAVVAERVEVPDGAVMMGVPAKQRQA
                     LDPVQQQRIGEISGRYIGVSRRYMSELKS"
     gene            4947..5720
                     /locus_tag="VFQ54_07430"
     CDS             4947..5720
                     /locus_tag="VFQ54_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0164862.1"
                     /translation="MAIDLERDGAVATIVFNAPERLNAMNREMLAELHAVLRDVAGDR
                     GIRAVILTGAGDRAFVAGADIKEMATLPRSESLAFARLAHATVTAIERLPVPVIAAVN
                     GYALGGGSEISLAADFRYAATNAVFAQPEVTLGIPPGWGGTQRLARAVGEARAAELIF
                     TGRRVSAEEALRIGLVNEVFPSEELLPAAKKTAEQIAANPRRVVQLSKRLIALTRQGN
                     PTSALAEEAFGFADVFETHDQREGMAAFVERRPAAFDHE"
     gene            5713..6501
                     /locus_tag="VFQ54_07435"
     CDS             5713..6501
                     /locus_tag="VFQ54_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEU0164863.1"
                     /translation="MSDVGGDNTARIAVLVKQIPDPGQIAIDAESRAPQYGGSLVVNT
                     FDTYAVAEAIDFKERYRADVTVVSVGPAPARDAILRGLATGADRGLHLVVDDLDRRDS
                     LAVATLIADALRDRGFDLILAGQISEDVETGQVGPQVAELLGIPHVSLVTGIEVDGAE
                     FRIRRDSEGRKQLVGVAMPCMALVLSGRDGEQRHPTLRGMMQAKRKPIEEIVVPSFDE
                     TPRMTWSDPVAPVRVRTAVIAQDVAPADAAKRLVGWLHEKKIVV"
     gene            6812..>7721
                     /locus_tag="VFQ54_07440"
     CDS             6812..>7721
                     /locus_tag="VFQ54_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEU0164864.1"
                     /translation="MTYIVVVADLADDGSMRPHTLELVTAARSIGDAFGASLAALVLA
                     PGREVAEAVAAIAGVDRVVLIESERALPYTSDVWSAAIGAALARLEPAAVIFAGSLAA
                     RDYAPRVAARHGWGMVADVHAIQIDGERLIVHRSLLGGRVESAIEFDGDAIPMMTIKP
                     GAFPRATSSATPAPIEALAIPRDEILNRVRVLESTLHRPATASLTSARRIVSGGRGLR
                     GPDAFGMLEELAALLDAAVGASGAVVGAGWRSHDDQVGSTGHTVAPLLYLAVGISGAP
                     QHLVGMQGSEYVVAINRDPDAPIFDIA"
BASE COUNT         1345 a         2301 c         2722 g         1353 t
ORIGIN      
        1 cgcgcgattc cgggctcgat gagtgaggaa gagcatgacg ggctcgagga agacctgcga
       61 ccgcacaagt acgaagccga cgacgaagtg atcggcgccg acgagcgcga gatgatcgac
      121 ggtattctca gcctcgagga gctgacggcg cgggacatca tggtgccgcg actcgacatc
      181 atcgccgtcg accgctttgt ctcgtcgcag gaattgatcg agacgatcac caaggcgggc
      241 cactcgcgaa tcccggttta ccaggaatcg atcgaccgcg tccttggcgt cctctacgcc
      301 aaggacctgc tgccgttcgt gatcgggagt acgagccgga ttccgctgct cgacctgatc
      361 cggccggcat tcgtcgtgcc cgagtcgaaa cgactcaacg aactcttcac ggagctgaag
      421 cgggcacgaa tccacatcgc gatcgtggcc gacgagtatg gcggcacggc cggcctggtc
      481 acgatcgagg acatcctcga ggagatcgtc ggcgagatcc aggacgagta cgacttcgag
      541 gaagagcagt ttcagctgct ggcgccggat gtgttgctcg cggacgggcg gcttccgatc
      601 gaggatgtcg aggatgcgct cggtgtcgac ttcgaggagg acgatgactt cgggacgctt
      661 ggcggcttcg tgcacaagca tctcggacgg cttccgatca agggagacgt cttcgaggcc
      721 gagggcgttc gcgtcgagat tctgggtgtc gagcgacatc gcgtccgcaa gctccggatc
      781 acgaagctgg cgccgcccga ggaagaggtc ggcagcggtc gccggggtgg gcgcggcaac
      841 gatcgcgacg aaccggacga gtcgacggaa tagcgcgctc gccggggcat tcccggacgg
      901 gaacgaccgc cggatcatcg acattgggca ggattgtgct gtgaatgacg tgcgtctcgg
      961 acgacctcgc ctccatctcc cgcgtaccgg cagcacgatg gacgatctcc tcgcgctcgc
     1021 ccgggtaggc gcgccggaag ggaccgttgt gacgacggac tttcaggatg ccgggcaggg
     1081 gagagcgggt cgcgtctggg cggctccgcc cggctcggcg ctcctgatgt cgacgctgct
     1141 caagcccggg cgccctcacg cggagttgac accgctttcg ctgctcgttg cgcttgcgat
     1201 tgcaacgtcg attcgtgacg ggttcgggct cgatgctcgc gtcaagtggc cgaacgatgt
     1261 ctttgtcggt cgtcgcaagg cggtcggcgt cctgctgcgc acgcacgcga tccgtggcga
     1321 atcgcagccc gtcgtcgtgg ttgggaccgg gatcaacgta aacgtacccg cggcttctct
     1381 cccggaaacc gggacgagca tcgccgtcga gctcggtcgc gaggtcgacc gggacgttgt
     1441 gcgcgatcgc gttcttgctg agcttggcga cgtctatcac cggtttcttc gtggagatat
     1501 cgaggctgac tgggatcggt tgaaccagtt gctcctgtat cgcgacgaac ccgtctcgat
     1561 ccaggatggc gatcggttgc tgaccgggac gctcgccggg ctcgatcacc gcggcgggtt
     1621 gctcctcatg cagcccgatg cgacgatgag gaccgtcgtt tcgggtgatc tcacccgggg
     1681 gccgcagccg atcggggatc ggagcgcctc ctgatcgcct gtatactttt gcgcgtgacg
     1741 tcagccaaag aacgcgcgaa gttgtgggtc gatgttgatc cgcgcagatg ggatgaggat
     1801 gaatttcgaa cttaccgacg atcagatcca ggttcgtgac atggtgcgcg agttcgcccg
     1861 gcgcgaggtc gctccccaca tccagcagtg ggatgcaaag ggtgaatacc accgcgagat
     1921 tatcgagaag atgggggagg ccggatttct cggtcttccg atccccgagg agtatggcgg
     1981 gcttgggctc gactacgtca gcctcgcgct cgcctgcgat gaactcgagt acgtggatac
     2041 atcgctccgc gtcgtgctca gcgtgcacgt cgggctcaac agcctggcgc tgctgcaatg
     2101 gggaaccgag gagcagaagc aacggtggct gataccgcag gcgaccggcg agaagatcgc
     2161 cacgttcggc ctgacggagc caggcgccgg ttccgatgcc gggtcgatcg cgacgactgc
     2221 cgtccgtgac ggtgattcct acattctcaa tggcaacaag atgtggatct cgctcgccga
     2281 tgtcgccgat cacttcctga tctttgccag cgtcgatcgt tccctgaagc atcgcggact
     2341 cgtcgccttc gtcgtcgagc gcgggatggt gggactgacg acggggacga tcaagggcaa
     2401 gctcggggtt cgcgccggta acaccggcga gatcgccttc aacaacgtcc gcgtcccgat
     2461 cgagaaccgg atcggcgagg agggcgaggg cttcaagatc gcgatgagtt gcatcgacca
     2521 ggggcgattc accgttgccg cgggtgcggt cgggctgacc cgggcttcgc tcgacgccag
     2581 cgttgcctac gcgaatcagc gtgagacctt cgggcagaag atcggtcgcc atcagctcgt
     2641 ccagcagatg atctcgaaca tggtgctcgg tcacgagacg tcgaagctgc tcgttatgca
     2701 ggcggctgag ctcaagaacc gggggatccg caacactaag gagacatcgc tcgcgaagtg
     2761 gcacgcgtgc gatgtcgcgt tgaagtcggc cgacgatgcc gtgcagatcc acggctcgta
     2821 tggcttctcg aacgagtatc cggtcgagcg cttcctccgg aacgcgcgcg gagccgtgat
     2881 ctacgagggg acgcgcgagg tccacaccat tcttcaggcg gaatacgcgc tcgggtaccg
     2941 cgaggacaag cctctcagtc acccgcagct gcccgcatcc ggattcgagg aggaatagca
     3001 atgggaaaca cggtcatcct cggcggcgca cggacgccgt tcgggaagct cagcggatcg
     3061 ctgggatcga agagcgcggt tgaactcgga acgatcgccc tcaacgcggc gatcgaccgc
     3121 tcgggtgtcg agcggcgatc gatcgagcac gtcacctttg gtcaggtcct gcagggtggc
     3181 gccggtcaga acccggcgcg acaagtgagt ttcgcggccg gactcgatcg cacggtgaca
     3241 tcggagacga tcaatcacgt ctgcggctcg ggtatccggg ccgttggtct cgccgacaac
     3301 gcgattcgcg tcggcgacca gaaggtgatc gccaccggcg gcatggagag catgacgaat
     3361 gcgccctaca tcctccgtgg cgctcgtggc ggctaccgga tgggtgacgg caagatcgag
     3421 gatatgatgg tcctcgacgg tctcttctgc gcgatcgagc acgagtcgat gggcgcgatc
     3481 tgcggcgtcg ttggcgacga gcatcacatc acgcgcgagg agcaggatgc ctgggcattg
     3541 cgatcccatc tgaaggcgct cgacgcgatc gactccggct ggatggcacg gcagatcgtg
     3601 ccagtcgaag tggccggtcg cagggagacg acgatcgtcg ataccgacga ggcgccgcgc
     3661 cgggacacga gcctcgaggc gctcgcccgg ctgaagccgg cattcgccgc cgccgggacg
     3721 atcaccgccg gcaatgcccc gggtgtgaac gatggggcga cggcgacgat catcgccgac
     3781 gcggagtggg cgagtgccga ggggcttccc gttcaggccc atatcctcgg gcatgcgcag
     3841 gcggcgtggg atgcgccgta tctcgcctac acgccggcga tggcgacgga gctcgccttg
     3901 aagaaggcgg ggctgaccgt cgccgacgtc gacctcttcg agatcaacga ggcgttcgcg
     3961 agtgtgacga tcatctcgac gcagcggctg gggatcgatc ccgagaaggt gaatatccac
     4021 ggcggggcaa tcgcgctcgg tcatccactg gctgcgagtg gggggcgcct gctgcttacg
     4081 ctcatcgagt cgttgaagga tatcggcggc ggaatcggtg tcgccgcaat ctgcactggc
     4141 ggcggacagg gagatgcgat cgtgattcga gtcgacggat gagtgaacga caacaacgtc
     4201 tcgccgcgct tgcggagcga caccccggtg cgatcatcct gcccttcgat ggtcactggc
     4261 cggtgattca cgagacggcg ttcatcgcgc cgggagcggt ggtgatcggc cgcgtgacac
     4321 tcggcgaaga aaccagcgtc tggttcaaca cgaccgtccg tggcgacatc gccccgatct
     4381 cgatcggcga ccggagcaat gttcaggatg gaacggtcgt ccacgtcaat gaggacgcac
     4441 ctgttgttgt cggcaacgac gtgacaatcg gtcacagcgc actgatccac ggcacggtga
     4501 tcggtgacgg gacgatgatc gggatgggtg cgattgtgtt gtcgtattcg aagatcggtt
     4561 cgggtgtcgt catcgccgcc ggcgccgtcg tcgcggaacg cgtcgaggtg ccggatggtg
     4621 cggtgatgat gggggttccg gcgaagcagc ggcaggcgct cgatcccgtg cagcagcagc
     4681 ggatcggcga gatcagcggc cgctacatcg gagtgagccg acgctacatg agcgaactga
     4741 agtcctgagg ggacgacagg agcgcggcca ctggaagcaa tcttcgggtg aactccaggc
     4801 gggacaggcc gtccggactg cggtccggca gcggcagtcc cgccccacga acgaccgggt
     4861 gcgccgcaga ctatcgtatt cgctcccgta cattttgcga cggagcagcg acgatgccgg
     4921 tatggtccgg gcatctggag gaattcatgg ctatcgatct cgaacgcgac ggcgctgtag
     4981 cgacgatcgt tttcaacgcg cccgagcgtc tcaatgcgat gaaccgggag atgctcgccg
     5041 agcttcacgc ggtgcttcgt gacgtcgcgg gcgaccgtgg aattcgggcc gtgatcctga
     5101 cgggagccgg cgaccgtgca ttcgtcgccg gtgcggacat caaggagatg gcgacactgc
     5161 cgaggtcgga gtccctggcg tttgcccgcc tcgcgcacgc gacggtgacg gcgatcgaga
     5221 gacttccggt gccggtgatc gcggcggtca acggttatgc gctcgggggt ggaagcgaga
     5281 tatcgctcgc ggccgatttc cggtacgcgg cgaccaatgc cgtcttcgcg cagccggagg
     5341 tgacgcttgg catccccccg ggttggggcg ggacgcaacg actcgcccgt gctgtcggtg
     5401 aggcgcgagc ggcggagctg atcttcaccg gtcgccgggt cagcgccgag gaagcgttgc
     5461 ggatcggcct ggtgaacgag gtcttccctt ctgaggagtt gttgcctgcg gcaaagaaga
     5521 cagcggagca gatcgcggcg aacccgcgtc gggtggtcca gctctcgaag cgtctgatcg
     5581 cgctcacgcg ccagggcaac ccgacatcgg cgctggcgga agaagcgttc ggattcgccg
     5641 atgtcttcga gacgcacgat cagcgcgagg ggatggccgc cttcgtcgag cggcgaccgg
     5701 cggccttcga tcatgagtga tgtcgggggc gacaacacgg cacggattgc cgtcctcgtc
     5761 aagcagattc ctgatccggg ccagatcgcg atcgatgccg aatcgcgtgc gccgcagtat
     5821 ggcggatcgc tggttgtgaa tacgttcgac acctacgccg tcgccgaggc catcgacttc
     5881 aaggagcgat acagggcgga cgtcaccgtg gtgtcggtcg gtccggcgcc ggcgcgtgat
     5941 gcgattctgc ggggtctggc gaccggagca gatcgcggcc tgcacctcgt ggtggacgat
     6001 ctcgaccggc gcgactcgct cgcggtggcg acgctgattg ccgatgcgtt gcgagatcgt
     6061 ggcttcgacc tgattctcgc cggccagatc tccgaggatg tggagaccgg ccaggttggc
     6121 ccccaggtcg cggagcttct cggcattccc cacgtctcgc tcgtgaccgg aatcgaggtc
     6181 gacggcgccg aattcaggat tcgacgggac agcgagggac gcaagcaact cgttggggtg
     6241 gcgatgccgt gcatggcgct cgtgctttcg ggacgggacg gcgagcagcg ccacccgaca
     6301 ttgcggggga tgatgcaggc gaagcggaag ccgatcgagg agatcgtcgt gccgtcgttc
     6361 gacgagacgc cgcggatgac ctggtcggac ccggtggcgc cggtgcgagt acgcacggcg
     6421 gtcattgcgc aggatgtcgc gcccgccgat gccgcgaagc ggctcgtcgg atggcttcat
     6481 gagaagaaga tcgttgtcta gggtgagcgc cagcggcaag acggtagggt cgggcgtcgt
     6541 tttgcggcgc cgccgacaaa tattgggcgc gatggcagga cgtcaaaggc gggacataga
     6601 agtcccgccc tacgaacgga cgctgtatgc gcactagtca tccgtaggac aggcttgtgt
     6661 cgcacatgac agcgacgctc ttggcaagac gccgtggtcg ggacgtcacg ttgcggctgg
     6721 agacaaactc cagccctaca aattccggtt gctctggcgt gcgtcgatcg attcgatatc
     6781 cgctctgcac gtggaatgga agggctgatc cttgacttac atcgtcgtgg tagccgatct
     6841 cgccgacgac ggatcgatgc gcccacacac gctggagctt gtgacggcgg cgagatcgat
     6901 cggggacgcg ttcggcgcgt ctctggccgc gctcgtactc gcgccggggc gagaggtggc
     6961 agaggcggtc gcggccatcg ccggcgtcga tcgcgtggtc ttgatcgagt ccgaacgggc
     7021 actgccgtac acgagtgacg tgtggtcggc ggcaatcggc gcggcactcg cgaggctcga
     7081 gccggcggcg gtgatctttg cgggaagtct cgccgcccgg gactatgctc cccgcgttgc
     7141 cgcccgccac ggctggggaa tggtcgccga cgtccatgcg atccaaatcg acggggagcg
     7201 gctgatcgtt catcggagcc tgctcggcgg acgcgtggaa tcggcgatcg agttcgacgg
     7261 cgacgcgatt ccgatgatga cgatcaagcc aggtgcgttt ccgcgtgcga catcctcggc
     7321 gactccggcg ccgatcgaag ctcttgccat cccccgggac gagatcctga acagggtcag
     7381 ggtgcttgaa tccacgctgc accggccggc gacagcgtcg ctcacgtcgg cgcggcggat
     7441 cgtctccgga gggcgtggtc ttcggggacc cgatgcgttt gggatgctcg aggagcttgc
     7501 cgcgctgctc gacgcggccg tgggtgcgtc gggagccgtc gtcggcgctg gttggcggtc
     7561 gcacgacgat caggtcgggt cgaccgggca cacggtcgcg ccgctcctct accttgccgt
     7621 tgggatcagc ggagcgccac aacacctcgt cggcatgcag ggttcggagt acgtcgtcgc
     7681 gatcaatcgg gatccggacg cgccgatctt cgacatcgca t
//
LOCUS       DASTMD010000510         1831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1922976, whole genome shotgun sequence.
ACCESSION   DASTMD010000510 DASTMD010000000
VERSION     DASTMD010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922976"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFQ54_07445"
     CDS             <1..414
                     /locus_tag="VFQ54_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU0164865.1"
                     /translation="AREAAKTPLANSERGDHDRTVALIHVRRRPDQFDADWRRGRALT
                     VRAAMDLAMTRMPESVAAHVNALSDPALAAFGLTQREEQVLRLIARRLSDREIAEELF
                     ISPRTVARHVAGILQKLDVHSRRDAGAIAIEHGFL"
     gene            596..>1831
                     /locus_tag="VFQ54_07450"
     CDS             596..>1831
                     /locus_tag="VFQ54_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164866.1"
                     /translation="MSRLRPLMTIVTIVMLWLGSFSGIAVAQGSSPPDLPAQAALADN
                     PTPASDTVDLPTDVPPPTDAPTEEAAPTDVVVPTEASQPTSEVTPTEAATSTATLTPA
                     PTGTAAAGSVVVQAHLCPATIAVADAAGSDLATACTGDAGGTVFDLAGNLGATASQTV
                     GASDGVEFDDLPADNYTITQEDAPSDSSLAVFCAAGTDGQSQQLEVDRGAIAFDLADQ
                     AQQVCDWYIGGATPVATANATATASGTPGLQAKNLPGTGSLQIKVYGCPEGYTQDSPD
                     PSGLARFCVVPMPGVGFTTFSNFTSTGTLHQGSTTGSPSFVKFDGLPTGTLSIREVFP
                     AGYTSAVVFCRAEGTPAVAYAQYPAASGGDHTNVYWGNLGEQDVSCDWYNIPALTTAE
                     NGTVYVNLNGCPTGFPADTV"
BASE COUNT          315 a          623 c          588 g          305 t
ORIGIN      
        1 gcgcgggaag ccgcgaaaac gccactggct aattcggagc gtggcgatca cgatcgcacc
       61 gtcgcgctga tccatgtgcg acgtcgcccc gaccagttcg atgccgactg gcgacgcgga
      121 cgcgcgctga cggtccgtgc ggcgatggac ctcgcgatga cgcgaatgcc ggagtcggtc
      181 gccgcgcacg tcaacgcgct atcggacccg gcgctggccg ccttcggtct gacccagcgt
      241 gaggagcagg tcctgcggct gatcgcgcgt cgactgtcgg atcgggagat cgcggaagaa
      301 ctcttcatca gcccgcggac ggtggcacgt cacgtcgcgg gcatcctcca gaaactcgat
      361 gtccactcgc gacgcgacgc gggtgcgatc gcgatcgagc acggcttcct ctgaccgctc
      421 ccgtacgcag gaattactca gggccgccga atgaaattgg gcaattcatt cgcatgacga
      481 gacctccggc gccgacgacg atggaaggaa tctgtccttt cgatcatcgc cgccgggtga
      541 tgtcggtcct gccatcgtgc gtgcgaccgg gctcacgcgg caaatggagc caatcatgtc
      601 gcgtcttcgc cctctcatga cgatcgtcac gatcgtgatg ctctggcttg gcagtttctc
      661 cgggatcgcg gtcgcccagg gcagttcgcc gccagatctt ccggcacagg cggcgctcgc
      721 cgacaatccg acacccgcat ccgacaccgt cgatcttccg accgacgtac cgcctcccac
      781 ggatgccccg accgaggaag ccgcaccaac cgatgtcgtt gtcccaaccg aagcctcgca
      841 gccgacaagc gaggtgacgc cgacggaggc cgcgacatcg accgcaacgt tgacgccggc
      901 gccgaccgga accgccgcgg ctggcagcgt cgttgtccag gcgcatctgt gtccggcgac
      961 gatcgccgtc gccgatgccg ccggcagtga cctcgcgacc gcctgcaccg gcgacgccgg
     1021 gggaacggtg ttcgatctcg ccgggaacct gggagcgacc gcgtcacaga cggtcggcgc
     1081 atccgatggc gtggaattcg acgatcttcc cgccgacaac tacacgatca cccaggaaga
     1141 tgccccgtcg gattcgtcgc tcgcggtctt ctgcgcggcg ggaacggatg gccagtctca
     1201 acagctcgag gtcgatcggg gtgcgatcgc cttcgatctc gccgatcagg cgcagcaggt
     1261 ctgcgactgg tatatcggcg gtgcgacgcc ggttgccacc gccaacgcta cggccactgc
     1321 cagcgggacg cccggtctgc aggcgaagaa cctgccgggt acggggtcgc tgcagatcaa
     1381 ggtctatggc tgtccggagg ggtatacgca ggattcgccc gatccgtctg ggctcgcccg
     1441 cttctgcgtc gtgccgatgc cgggagtcgg gttcaccacc ttcagcaact tcacctcgac
     1501 cggaacgctg catcaggggt cgacgaccgg aagtccgagt ttcgtcaagt tcgacggtct
     1561 tcccacgggc acgctctcga tccgcgaggt ctttcccgcc ggctacacct cggccgtcgt
     1621 tttctgccgg gcggagggaa cgccggcggt cgcctacgcg cagtacccgg cggcgagcgg
     1681 gggcgaccac acgaatgtct actgggggaa tctcggagaa caggatgtct cctgcgactg
     1741 gtacaacatc ccggcactca ccacggctga aaatggcacg gtctacgtca acctgaacgg
     1801 ttgtcccacc gggtttccgg cagacaccgt c
//
LOCUS       DASTMD010000511         1931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1030303, whole genome shotgun sequence.
ACCESSION   DASTMD010000511 DASTMD010000000
VERSION     DASTMD010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030303"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFQ54_07455"
     CDS             <1..334
                     /locus_tag="VFQ54_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0164867.1"
                     /translation="VTPIPIQHGTLPIVGYRIGNFAYITDAKVIPDIARPLLKGVDTL
                     VITALRKTPHPAHMSLGEALRAIDEIAPRQAFLSHIAHDMGRHADVELELPPHVRFAT
                     DGMVVQIA"
     gene            485..1585
                     /locus_tag="VFQ54_07460"
     CDS             485..1585
                     /locus_tag="VFQ54_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0164868.1"
                     /translation="MTIEPDVRVAAVVHSTVAKPRLPQLKFLPEIAAVLLVITWASTF
                     ILTKDAYNQITPMAYSFARYLSASLLGLVVLFLRGWRTPARRATYWRIDRSDFWRFTW
                     CGLFGFAIYQVCFSVGLAHTSAFASSLLTSLIPLFSLILVTSLGERPARPVWLGVIVA
                     IAGVTLFLAQGGGGSGMWGNLLCLGAAACFAVYSEIIRPLVKKYPAETVAAYTTFFGS
                     IPLFAIAMPEVLRQDWTGLEPKIWLMVAYTVTLPIYLALIVWNWIISQRGVAATGWNL
                     LVPIASGIMAMIFLGDSLVPIQVVGALLALCGLILMQRGSLVARTRQRTPDPGPPGRK
                     HIPSSDLGSAIDRFAFPRPMASELPCARTLCR"
BASE COUNT          297 a          629 c          607 g          398 t
ORIGIN      
        1 ggtgacgccg atcccgatcc agcacgggac gcttccgatc gtcggctacc ggatcgggaa
       61 cttcgcctac atcaccgacg cgaaggtgat cccggatatc gcacggccgc tgctgaaggg
      121 cgttgacacg ctcgtgatta cggcgctccg gaagacgccg catcccgcgc acatgtcgct
      181 cggcgaggcg ctgcgggcga tcgacgagat cgcgccacgg caggcgtttc tttcgcacat
      241 cgcgcatgac atggggcgcc acgccgatgt cgagctggaa cttcctcccc acgtgaggtt
      301 cgcgaccgac ggaatggtgg tgcagattgc ctgatcccgt cataatgaat gcctgaaatc
      361 gtcgcgatcg tggcgggtcc aaacagatat ccccacgagc ggccggctct ccgaatcccg
      421 gatgggccgg cttttctgat gtacgcggaa tgacacgatg agcgccgaac cgatggctgc
      481 agacgtgaca atcgaacccg acgttcgcgt cgccgcggtc gtgcattcga ccgtcgcgaa
      541 gccgcgtctg ccgcaactga agttcctgcc cgagatcgcc gctgtcctgc tcgtcatcac
      601 ctgggcgtcg acgttcatcc ttacgaagga tgcctacaac cagatcacgc cgatggccta
      661 ttcgttcgcg cgatatctct cggcgtcact gctcggactc gtcgtgctct tcctccgcgg
      721 ctggcggaca ccggcgcggc gcgcgaccta ctggcggatc gaccggagcg acttctggcg
      781 cttcacctgg tgcgggctct tcggcttcgc gatctaccag gtctgcttct cggttggact
      841 cgctcatacg tcggcattcg ccagttcgtt gctgacgtcg ctgatcccgc tcttttcgct
      901 gatcctcgtc acatctctcg gagagcggcc ggcgcgaccg gtctggctcg gcgtgatcgt
      961 cgcgatcgcc ggtgtgacgc tctttctcgc gcagggaggg ggaggatccg ggatgtgggg
     1021 gaatctcctc tgccttggcg ccgccgcctg tttcgcggtc tacagtgaga tcatccgacc
     1081 gctggtgaag aagtacccgg cggagaccgt cgccgcctat acgaccttct tcgggtcgat
     1141 cccgctcttc gcgatcgcga tgccggaggt cctccgccag gactggacgg ggctggagcc
     1201 gaagatctgg ctgatggtcg cctacacggt gacgttgccg atctatcttg cactgatcgt
     1261 ctggaactgg atcatttcgc agcgcggcgt cgcggcgacg ggctggaatc tcctcgtccc
     1321 gatcgcgagc ggcatcatgg cgatgatttt cctcggggat tcgctcgtcc ccatccaggt
     1381 cgtcggggcg ctccttgcgc tctgtgggtt gattctgatg caacgcggca gcctcgtggc
     1441 acgaacccgg cagcggacgc ctgatcccgg cccgcccgga aggaagcaca ttccgtcatc
     1501 ggaccttgga tccgccatcg acagattcgc cttcccccgt ccgatggctt cagagcttcc
     1561 ctgcgcacga acgctgtgtc gatgattcgg cgtgggcatg gcgtggtcga gcggatctcc
     1621 tttgcgcctt cggccccagc accaaatcta tgtatggtgg tcgcgcgtac ggcggctctg
     1681 aagctgtcag cccgactggg cacatttccg cgaacatggt gcagggctga tcggcggaat
     1741 gtgccgcctc gacggtcgcg gacgcgggat cggctccgcc gacccggtgc gaccaccagg
     1801 gtcgcgtcta tggttcccct ggcgactgtg ctctcctgct acgggaagcc ctgaagtcgc
     1861 gccgcctgct agaatcccgt ctgtgaagat tgcgcttgtc tcaccatatg gcatgcaact
     1921 ggctggcggc g
//
LOCUS       DASTMD010000512         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_135320, whole genome shotgun sequence.
ACCESSION   DASTMD010000512 DASTMD010000000
VERSION     DASTMD010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135320"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFQ54_07465"
     CDS             complement(<1..450)
                     /locus_tag="VFQ54_07465"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU0164869.1"
                     /translation="MLRAFIAIVASVGLLAPVAMAQDATTTPTTFDPSAFNVGFEQIG
                     SGFSKPVFITNAGDDSGRLFVVDQVGQIQIIQDGDVLPTPFLDIVPLVNSSSQELGLL
                     GLAFAPDYATSGYFYVDYITQDMHTVVVRYSVSKDDANVADPDSAVTI"
     gene            complement(682..1734)
                     /locus_tag="VFQ54_07470"
     CDS             complement(682..1734)
                     /locus_tag="VFQ54_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU0164870.1"
                     /translation="MQRNGRTRASGRGWLWVSLVSLIVSVWPGASANAAAPSFFASLD
                     QSLSTMAPASNFLVAEFSGNSCATIHGRNQDDALAIASTFKLYVLGELARQIQLGMAS
                     WDEKITLQADLRSMPSGDYAYAAAGTTATIRDLAEAMIWNSDNTATDNLINRLGRDNV
                     QRAFAAFGNQEAVRNTPLLLTHEMFAIKMTQSSTWMAQYEKADDNARLDMIKQDIDPL
                     TIDPTAGWGLWNGPTAISGIEWFASASDLCRVMAQLWTMGAQPGLSPVRDILSGNRYG
                     ITDTKTWPRAGYKGGYESGVVNMTFVLQRSDGRVFFVSAGYNDPTGLVDTATARSYLD
                     PLFACLGSGKTGDCGA"
     gene            1994..>2548
                     /gene="lepB"
                     /locus_tag="VFQ54_07475"
     CDS             1994..>2548
                     /gene="lepB"
                     /locus_tag="VFQ54_07475"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU0164871.1"
                     /translation="MPDAAPNLPSPGATDPAELTPPARRRRRRRTWRRELIELCLIVI
                     VVFVGMRMFLLPYQVDGASMTPYLSNGERLFVNRTAYAHIDLNAVLDLLPGEKHIDTN
                     EFYPFSAPQRGDVIVLTPPTTSKEPYVKRIIGLPGETIAFHEGRVYVNGQPLAESYID
                     GAITYCYYGRWCSLKVPDDSVYVLG"
BASE COUNT          497 a          787 c          805 g          459 t
ORIGIN      
        1 gatcgtcacc gcgctgtccg gatcggcgac gttcgcatcg tccttggaca ccgaatagcg
       61 gacgacgacg gtatgcatgt cctgcgtgat gtagtcgacg tagaagtagc cgctcgtcgc
      121 gtagtccggc gcgaaggcca acccgagcag accaagttcc tggctcgacg agttcacgag
      181 cggaacgata tcgaggaacg gtgtcggcag gacgtcccca tcctggatga tctggatctg
      241 cccgacctga tcgacgacga agagccggcc gctgtcgtcg ccggcgttcg tgatgaagac
      301 gggcttgctg aatccgctgc cgatctgctc gaatcccacg ttgaaggccg aaggatcgaa
      361 ggtggtcggg gtcgtggtgg cgtcctgcgc catcgcgacc ggagcaagca ggccaacgga
      421 ggcgacgatg gcgatgaagg cgcggagcac tcgggctcca ggggcgtgaa aatggagacg
      481 catcgcggga agtcccttca ggtagaggtc gtagagtcat gacgacacga cgagcgacat
      541 gctatcgagc gggcacccca ccattgtcca cgccggatct gcaatccgtc accccgaata
      601 ggaacaattt cacatgggga tcgccccgat cgcgacccct ctctggcccg ggcccatgac
      661 gcggagcgat gcacggcgcg atcaggcgcc gcaatctccg gtcttgccgg aaccgagaca
      721 ggcgaagagc ggatcgagat aggagcgcgc ggttgccgtg tcaacgagcc cggtagggtc
      781 gttgtagccg gccgagacga agaagacgcg gccgtcgctc cgctggagca cgaaggtcat
      841 gttgacgacg cccgattcgt acccgccctt gtagccggct cgcggccacg tcttcgtatc
      901 cgtgatcccg tagcggttgc cggagaggat gtcgcggacc ggggaaagtc caggctgggc
      961 gcccatcgtc cagagctgcg ccatcacccg gcagagatcc gatgccgagg cgaaccactc
     1021 gatgccactg atcgccgtcg gaccgttcca gagtccccag ccagccgtgg gatcgatcgt
     1081 gaggggatcg atgtcctgct tgatcatgtc gagccgggcg ttatcgtcgg ccttttcgta
     1141 ctgggccatc cacgtcgacg attgcgtcat cttgatcgca aacatctcat gggtgaggag
     1201 aaggggtgta ttgcgcacgg cttcctgatt gccgaacgcg gcaaacgcgc gttgcacgtt
     1261 gtcccggccg aggcggttga tcaggttgtc ggtcgcggtg ttgtcactgt tccagatcat
     1321 cgcctcggcg agatcccgga tcgtcgccgt cgtgccggcc gcggcgtatg catagtctcc
     1381 cgacggcatg ctgcgcagat cggcctgcaa ggtgatcttc tcgtcccaac tcgccatgcc
     1441 gagctgaatc tgccgcgcga gctcgccgag gacatagagc ttgaatgtcg aggcgatggc
     1501 gagcgcgtca tcctgattgc gaccgtgaat cgtcgcgcag gagttgccgc tgaactcggc
     1561 gacgaggaaa ttgctcgccg gcgccatcgt cgacagcgac tggtcgagcg acgcaaagaa
     1621 gcttggcgcc gccgcgttcg ccgacgcacc cggccagacc gaaacaatga ggctgacaag
     1681 tgaaacccac agccatcccc gtccggatgc cctggttcgt ccgttgcgtt gcatgaccgt
     1741 gcccaccgcc gtgttagctg catgatgttc gaatgggtga agcgggagcg cgggagaacc
     1801 cggaaggcag tggcccatgc ctctgcatgg agtgtccgta cagtctgcat gccaaggcga
     1861 tcatattctt tggaaggcgg agagttcaag atgtgccatg cagtcaggca cgttctgtag
     1921 attcacgaaa atagctgccg gtatactgac cgcgatgaac gattcattcc gagaacagaa
     1981 aacgagggaa tcgatgccgg acgcggcacc caatctgcca tcacccggag cgaccgatcc
     2041 cgcggaattg acgccacccg cgcgtcgacg ccggcggcgc cgaacctggc gacgcgaact
     2101 gatcgagctc tgtctgatcg tgatcgtggt cttcgtcggg atgcggatgt tcctgttgcc
     2161 gtaccaggtg gacggcgcga gcatgacccc atatctcagc aacggcgaac ggctcttcgt
     2221 gaaccgcacc gcctacgccc acatcgatct caacgccgtg ctcgatctgc ttcctggtga
     2281 gaagcacatc gacacgaacg agttctaccc gttcagcgcc ccccaacgcg gtgacgtgat
     2341 cgtcctcaca cccccgacga catcgaagga gccctacgtc aagcgcatca tcggccttcc
     2401 cggcgaaacg atcgccttcc acgagggccg cgtctatgtc aacggccagc cgctggccga
     2461 gtcatacatc gacggcgcga ttacctattg ctattacggt cgctggtgct cgctcaaggt
     2521 tccggacgac agcgtctacg tgctcggc
//
LOCUS       DASTMD010000513         4851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_135404, whole genome shotgun sequence.
ACCESSION   DASTMD010000513 DASTMD010000000
VERSION     DASTMD010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135404"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1200
                     /gene="tuf"
                     /locus_tag="VFQ54_07480"
     CDS             1..1200
                     /gene="tuf"
                     /locus_tag="VFQ54_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU0164872.1"
                     /translation="MAKKVFERTKPHVNIGTIGHVDHGKTTLTAAISKTLSLQGGAAF
                     RDFASIDNAPEERARGITIATSHVEYETANRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAPDGPMPQTREHILLARQVQVPNIVVFLNKVDMMDDEELLELVELEVRELLSQ
                     YDFPGDEIPIIRGSGLAALESQSSDLSSPEYLPILELMQAVDDYIPTPERAVDLPFLM
                     PIEDVFGIKGRGTVVTGRIERGRVKTGDEIEIVGMGERRKVIVTGVEMFMKTLESGDA
                     GDNVGTLLRGVERDDVQRGQVLAKPGSINPHTKFQAEVYVLSKEEGGRHTPFFPGYRP
                     QFYIRTTDVTGSIELPEGTEMVMPGDNIQMGVELITPVAIEEGLRFAIREGGRTVGAG
                     AVTQITQ"
     gene            1312..1482
                     /gene="rpmG"
                     /locus_tag="VFQ54_07485"
     CDS             1312..1482
                     /gene="rpmG"
                     /locus_tag="VFQ54_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU0164873.1"
                     /translation="MAKKSKVDRPVVTMECTVCRERNYTTEKNRRNDPGRLELKKFCP
                     RCRQHQLHRETR"
     gene            1502..1574
                     /locus_tag="VFQ54_07490"
     tRNA            1502..1574
                     /locus_tag="VFQ54_07490"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1535..1537,aa:Trp,seq:cca)
     gene            1750..2193
                     /gene="secE"
                     /locus_tag="VFQ54_07495"
     CDS             1750..2193
                     /gene="secE"
                     /locus_tag="VFQ54_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU0164874.1"
                     /translation="MSTQEQAVPKRRSSRGATANRRSAAQRAAAGAIAEVPATEATAA
                     SATGDKKAVAARSTPAAEPAPRKDRAARKEQSAEPRKGVVNVERFEPIRRFTRDTMAE
                     IKKVNWPDQQTTRNLTIVVIAVSAGLGLILGGMDFVLLKIFEALP"
     gene            2234..2833
                     /gene="nusG"
                     /locus_tag="VFQ54_07500"
     CDS             2234..2833
                     /gene="nusG"
                     /locus_tag="VFQ54_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU0164875.1"
                     /translation="MTRQTLRERLAAKRAAEIGGSESEVQGHWYVLHTYSGYENKVKK
                     TIESRIEALDMGDRVFEIVVPTQEEIEIRQGQRHNVQRKVFPGYVLVRMEMDEDSWYQ
                     LRNTPGVTGFVSFNNKPVSLTEDEAQGVLKGMAAEAPKVKMQFALGDTVRIVDGPFAD
                     FRGEIDEINNEKGKIKVLVSFFGRETPVELDFLQAERET"
     gene            2930..3355
                     /gene="rplK"
                     /locus_tag="VFQ54_07505"
     CDS             2930..3355
                     /gene="rplK"
                     /locus_tag="VFQ54_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU0164876.1"
                     /translation="MAKKIRGYVKLQIPAGKATPAPPIGPALGQHGVAIMNFCKEYNE
                     RTQAQVGQIIPVVITVYEDRSFTFVTKTPPAADLLRKAAGVKSGAANPRTTKVGSVST
                     QQVREIAELKQKDLNAVDLAGAIKQIEGTARSMGIEVTA"
     gene            3424..4146
                     /gene="rplA"
                     /locus_tag="VFQ54_07510"
     CDS             3424..4146
                     /gene="rplA"
                     /locus_tag="VFQ54_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU0164877.1"
                     /translation="MAKHGKNYVAALAQVDQDREYRVEDAVELLQKVSFAKFDETIEL
                     HARLGIDPRQADQAVRTNVNLPHGSGSTVRILVFAAGEAERVALEAGADYAGTDELVQ
                     QIESGWLEFDAAIAMADQMSKVGGLGRILGRRGLMPNPRSGTVIREVEDLPNVIRELK
                     GGRVEFRNDRTGNVHITVGRKGFTPEQLTTNVFAAIDAVNRAKPTGIKGIYCRTLTIT
                     STMGPGITIDVNDALGRASSVV"
     misc_feature    4159..4291
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            4337..>4851
                     /gene="rplJ"
                     /locus_tag="VFQ54_07515"
     CDS             4337..>4851
                     /gene="rplJ"
                     /locus_tag="VFQ54_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU0164878.1"
                     /translation="MPTPKKATEIAELADRFNRAQLAVIADYRGLSVTQLQDFRGRIR
                     NMDSEFRVAKNTLTRIASEQAGIEGLAAQLEGPTAILFAFGDAVAPAKALSDFARASR
                     ILTVRAGVMNGQLLSPEDVEAVATLPPREELLGKLVGMLASPMARTVGVLSGPSRSVA
                     YLLNSRLEALGG"
BASE COUNT          969 a         1391 c         1662 g          829 t
ORIGIN      
        1 gtggcgaaga aggtatttga gcggacgaag ccgcatgtga acatcgggac gatcgggcac
       61 gtggaccacg ggaagacgac gctgacggcg gcgatctcga agacgctgtc gctgcagggc
      121 ggggcggcgt tccgggactt cgcgagcatc gacaacgcgc cggaagagcg ggcgcgcggg
      181 atcacgatcg cgacgagcca cgtggagtac gagacggcga accggcacta cgcgcacgtg
      241 gactgcccgg gacatgccga ctacatcaag aacatgatca ccggggcggc ccagatggat
      301 ggcgcgatcc tggtggtgag tgcgccggac ggaccgatgc cgcagacgcg ggagcacatc
      361 ctgctggcgc ggcaggtgca ggtgccgaac atcgtggtct tcctgaacaa ggtggacatg
      421 atggacgacg aggaactgct ggagctggtg gagctcgaag tgcgggaact gctgagccag
      481 tatgacttcc cgggcgacga gattccgatc atccgggggt cggggctggc ggcgctggag
      541 agccagagca gcgatctgag ctcgccggag tacctgccga tcctggagct gatgcaggcg
      601 gtggacgact acattccgac gccggagcgg gcggtggacc tgccgttcct gatgccgatc
      661 gaagacgtgt tcgggatcaa ggggcggggc acggtggtga ccgggcggat cgagcggggc
      721 cgggtgaaga cgggcgacga gatcgagatc gtggggatgg gcgagcgccg gaaggtgatc
      781 gtgaccgggg tcgagatgtt catgaagacg ttggagtcgg gggatgccgg cgacaacgtg
      841 gggacgctgc tgcggggcgt ggagcgggac gacgtgcagc gggggcaggt gctggcgaag
      901 ccgggctcga tcaacccgca cacgaagttc caggccgagg tgtacgtgct gagcaaggaa
      961 gagggggggc ggcacacgcc gttcttcccg gggtaccggc cgcagttcta catccggacg
     1021 acggacgtga cggggtcgat cgaactgccg gaaggcacgg agatggtgat gccgggggac
     1081 aacatccaga tgggggtgga gttgatcacg ccggtggcga tcgaggaagg gttgcggttc
     1141 gcaatccggg aaggtggccg cacggtgggt gctggcgccg tcacccagat cacccagtag
     1201 tttcgcttga aaatgatccc cgggggtggg tttccgcccc cggggaagtc cagaactccc
     1261 gccggttcgc ccgcggtgac gactgggtag ttctggaaag gatgtagtac aatggcaaag
     1321 aagtccaagg tcgatcggcc tgtggttacg atggagtgca cggtgtgccg agagcgtaat
     1381 tacacgaccg agaagaaccg gcgcaatgat ccgggtcgac ttgagctcaa gaagttctgc
     1441 cctcgatgcc gacagcatca gctgcatcgc gagacgcggt aggctacggg gagagcggca
     1501 tagggtcgta gctcaattgg tagagcggcg gtctccaaaa ccgtaggttg cgggttcgat
     1561 tcctgccggc cctgcccggt gatccagtcc gagtcgaggt ggggcggagc cgaacgtggc
     1621 acgccccact tctgtgtgcg atagacagat gtttcattcc gaaggcatcg cgcaatcact
     1681 cgtcgggcgg tagagttgtc gttcgaatga tcctcgaact atcccgatgt ccgtgaagga
     1741 ttccgagagt tgagcactca agaacaggcc gtgcccaaga gacgatcgtc ccggggcgcg
     1801 acggcaaatc ggcggtccgc cgcccagcgt gctgcagcag gtgcgatcgc ggaagttccc
     1861 gcaacggagg cgaccgcggc atccgcgaca ggcgacaaga aggctgtcgc ggctcgcagc
     1921 acgccggcgg cagagccggc gccacgcaag gaccgcgcgg cgcgcaagga acagtctgcc
     1981 gagccccgga agggcgtcgt caacgtcgag cgcttcgagc cgatccggcg cttcactcgt
     2041 gacacgatgg ccgagatcaa gaaggtgaac tggcccgacc agcaaaccac gaggaatctc
     2101 acgatcgtgg tcatcgcggt gtcggcgggg ctgggcctga tccttggtgg tatggacttc
     2161 gttcttctca agatattcga ggcactgccg taggacatct tcgcagtagt tccgtgatgt
     2221 gaaagaggtt ccgatgacac ggcaaacgct ccgggaacgt ctcgccgcga aacgggccgc
     2281 cgagattggt gggtccgaat ccgaggttca gggacactgg tacgtgttgc acacgtactc
     2341 cggatatgaa aacaaggtca agaagacgat cgagagccgg atcgaagccc tggacatggg
     2401 cgatcgggtt ttcgagatcg tcgttcctac ccaggaagag atcgagattc gccaggggca
     2461 gcggcacaac gtacagcgga aggtctttcc ggggtacgtg ctggtgcgca tggagatgga
     2521 cgaggattcc tggtatcagc ttcggaacac acccggtgta accgggtttg tcagcttcaa
     2581 caacaagccg gtctccctca ccgaggacga ggcgcagggc gtattgaagg ggatggccgc
     2641 cgaggcgccc aaggtcaaga tgcaatttgc ccttggcgat acagttcgga ttgtcgacgg
     2701 tcccttcgcg gatttccgcg gagagatcga tgagatcaac aacgagaagg gcaagatcaa
     2761 ggtgctcgtc tcgttcttcg ggcgtgagac tccggtcgag ctcgattttc ttcaggccga
     2821 acgggaaacg tgaggcgctg aacgcagttt tgtggatacg gtgtcacgcg gcactgccgc
     2881 gtgacattcg gtttgtttgg gatgtggtac ccaggaaagg acacattcgt tggctaagaa
     2941 gattcgaggc tacgtcaagc ttcagattcc ggcgggcaag gcaactccgg ccccgccaat
     3001 cggtcccgcg ctcggtcagc acggtgttgc gatcatgaac ttctgcaagg agtacaacga
     3061 gcgcacccag gcccaggtcg ggcagatcat cccggtcgtg atcacggtct acgaagaccg
     3121 ctcgttcacg ttcgtcacga agaccccgcc ggcggcggat ctccttcgca aggcggccgg
     3181 tgtgaagagc ggcgcggcga acccgcgcac gaccaaggtt ggttcggtct ccacgcagca
     3241 ggtccgcgag atcgcggagc tgaagcagaa ggacctcaac gcggttgacc tcgccggtgc
     3301 gatcaagcag atcgagggca ccgcgcgcag catgggaatc gaagtcaccg cctaggttga
     3361 cgcgatttcc cctaacaggt gggagggctg acacagcccg tttgcaccac cggaggcaaa
     3421 gacatggcaa agcacggcaa gaactatgtg gcggcactcg cccaggtcga tcaggaccgc
     3481 gagtaccggg tcgaggatgc ggtcgaactc ctgcagaagg tttccttcgc gaagttcgat
     3541 gagacgatcg agcttcacgc ccggctcggg atcgatcctc gccaggccga ccaggccgtt
     3601 cgtacaaacg tcaatcttcc ccacggctct ggtagcacgg tccgtatcct cgtcttcgcc
     3661 gcgggtgaag ccgagcgcgt cgccctcgaa gccggcgccg actacgcggg taccgacgaa
     3721 ctcgtccagc agatcgagtc cggctggctc gagttcgacg ccgccatcgc gatggcggac
     3781 cagatgagca aggtcggtgg tctcggccgc atcctgggtc gccggggcct catgccgaat
     3841 ccgcgctctg gtacggtcat tcgcgaagtc gaggatctgc cgaacgtcat tcgcgaactc
     3901 aagggtggtc gtgtggaatt ccgcaacgac cgcaccggca acgtgcatat caccgtcggc
     3961 cgcaagggat tcacccccga gcagctgacg acgaatgtct tcgcggcgat cgacgcggtc
     4021 aaccgcgcga agccgactgg catcaagggt atctactgcc gtacactgac gatcacgtcg
     4081 acgatggggc ccggcatcac tatcgatgtc aacgacgcac tcggtcgggc atcctccgtc
     4141 gtctagtcat ttgccaacca acaagagtct tcctggccga agacagccgg cgcggttctc
     4201 cgcttaattc ccggccgagg ctcagatgct cactttcgcg agaatgtgcg ccctgggctg
     4261 tgtccgccca gggcgctctt ttgtgaggaa caggggatct tccccgaaaa tctttgcgga
     4321 aaggaggaaa agatcaatgc caacaccaaa gaaggctacc gagatcgcgg aacttgccga
     4381 ccggttcaac cgggcgcaac tcgccgtgat cgcggactat cgcggtctct ccgtgaccca
     4441 gctgcaggac ttccgtggca ggattcgtaa catggactcc gagttccgtg tcgcgaagaa
     4501 caccctgacc cggatcgcgt ccgagcaggc ggggatcgag gggcttgccg ctcaactcga
     4561 ggggccaacc gcgatcctct tcgcgttcgg tgacgccgtc gctcctgcga aggcgctttc
     4621 ggatttcgct cgtgcgtcgc gcatccttac ggttcgcgcc ggtgtgatga atggccagct
     4681 cctctcgcca gaggatgtcg aggccgtcgc cacgctgccg ccacgcgagg aattgctcgg
     4741 caagctcgtg ggaatgctcg catccccgat ggcgcgcacg gtgggcgttc tcagcgggcc
     4801 gtcccgctcc gtcgcctatc tgctcaactc acggctcgag gccctgggtg g
//
LOCUS       DASTMD010000514         1846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1478334, whole genome shotgun sequence.
ACCESSION   DASTMD010000514 DASTMD010000000
VERSION     DASTMD010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478334"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFQ54_07520"
     CDS             complement(<1..509)
                     /locus_tag="VFQ54_07520"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU0164879.1"
                     /translation="MLALLLLAGVASTSSSRVAAAGVVAQNVTFSTGADTPYTGTLAG
                     KASGGLEPYSYYLDAGPTHGTLTQPLSDTGAFTYTPSAGYNGLDSFTYHAEDGDGRSS
                     PIATVKLEVGSPPLTLNGVAGPVTVPVGSTVIPGSDSGAVIAFWSNPTCSGTPFLAHI
                     IPGGTVTIAR"
     gene            748..900
                     /locus_tag="VFQ54_07525"
     CDS             748..900
                     /locus_tag="VFQ54_07525"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEU0164880.1"
                     /translation="MLFIIAAVLVVLWLVGWLAFHVAGAFIHLILIVALIVIIVQLLQ
                     GRRSAS"
     gene            1080..1754
                     /locus_tag="VFQ54_07530"
     CDS             1080..1754
                     /locus_tag="VFQ54_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU0164881.1"
                     /translation="MVAPAAKKRVVAKPKLPARGTAVAVGIDRIDDEVTLTGVSLKQL
                     DLRDRELTQLALKKADLGMVTFAGAHLFGLALEDARLDRCDLANTAVEQGRFVRVELD
                     GCKLTGAVLNRSLVQDVRFVECRADYAQWQGINTQRVDFINCNLDHAYFNDADLTGVR
                     FRDCDLRNADFSHAQLAGADLRGSNLENIVITVDQLRGMKVTTDQAIYLCGLIGLEID
                     DEVGLL"
BASE COUNT          332 a          584 c          591 g          339 t
ORIGIN      
        1 cgcgcgatgg taacagtgcc ccccggtatg atgtgggcga ggaatggcgt gcccgagcag
       61 gtcggattgg accagaacgc aatcacggca ccggaatcgc tcccgggaat gacggtgctt
      121 cccactggta ccgtgaccgg accggcgaca ccgttgagcg tgagcggcgg tgaaccgacc
      181 tcgagcttga cggtggctat ggggctcgaa cgaccatcgc cgtcctcggc gtgataggtg
      241 aaggagtcca atccgttgta gccggcgctc ggggtatagg tgaacgctcc cgtatcactc
      301 agcggttgcg tcagggtgcc gtgcgtcggc ccggcatcga ggtaatagga gtacggctcc
      361 agaccgccgc ttgccttgcc ggcaagcgtc cccgtgtacg gggtatcggc ccctgtggaa
      421 aaggtcacat tctgggcgac aacgccggcc gccgcgactc gtgatgatga cgtggatgcg
      481 actccggcaa gcaggagcag ggcaagcacg accgctgtga tccatcgcca ttgccatcgt
      541 ggcgccgagt acgctgacat cattgcttcc ccacagatcc tggctatgcg ttctacattg
      601 tgtgcttatg acggcactgg agtcccagtt cgcacgatac cggagaacac gtgaacggtg
      661 caaccaggtg tcccgtcaga acggcggcac ggtatctgca tccggggtac catgacagat
      721 tcctggaaaa gagaagggac cctggagatg ctctttatca ttgccgcggt gctcgtcgtc
      781 ctctggctgg tcggctggct ggcgttccat gtggcgggcg ccttcattca tctcatcctc
      841 atcgtggcgc tgatcgtcat catcgtgcag cttctccagg gacgccggtc tgcctcctga
      901 cctttccgta tcggcatgaa tgtgatcgcg gagcagcgtg ccggacggcg tggaaacgtc
      961 gcccggcacg ctgctccacg gacgtcgcca gaacgcggcg aatctgcgat catcgcatac
     1021 tcgggcaacg atcgctggcg acaacgtgcg gcgtcggatc tggattcagg agagcgagta
     1081 tggtggcacc cgcggcgaag aagcgtgttg tggcgaaacc gaagctcccg gcccgcggca
     1141 cggccgtcgc ggtcgggatc gatcgcatcg acgacgaggt gacgttgacc ggcgtctcgt
     1201 tgaagcagct cgatctccgc gaccgcgaac tcacccagct cgcgctgaag aaggcggacc
     1261 tcggcatggt caccttcgcg ggcgcacacc tcttcggact cgcgctggaa gatgcccgcc
     1321 tcgaccgctg cgatctcgcc aacaccgcgg tcgagcaggg gcgtttcgtc cgggtcgagc
     1381 tcgacggctg caagctgacg ggcgccgtcc tcaaccgctc gctcgtccag gacgtccggt
     1441 tcgtcgaatg ccgtgccgac tacgcgcagt ggcaggggat caacacgcag cgggtcgact
     1501 tcatcaactg caacctcgac cacgcctact tcaatgatgc cgacctgacg ggtgtgcgct
     1561 tccgggattg cgatctccgc aacgcggatt tctcccacgc ccagcttgcc ggggccgacc
     1621 tccgtggctc gaatctcgag aacatcgtga tcaccgtcga ccagctgcgc ggcatgaagg
     1681 tcacgaccga ccaggcgatc tatctctgcg gcctgatcgg cctcgagatc gatgacgagg
     1741 tcggcctgct ctgaggaccg gagcgaagcc ggtccgatcg tcacgcccgg ctgaccaggt
     1801 tcgcatgcag ttccgacacg atctcatcga gccgatccag ctcggt
//
LOCUS       DASTMD010000515         3851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2191567, whole genome shotgun sequence.
ACCESSION   DASTMD010000515 DASTMD010000000
VERSION     DASTMD010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2191567"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VFQ54_07535"
     CDS             complement(<1..611)
                     /locus_tag="VFQ54_07535"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU0164882.1"
                     /translation="MAATTQDVENMDIAVEPGDDRAYELARMRHSAAHLMAEAVQELF
                     PDARFGIGPAIDNGFYYDFDLPRALTPDDLVEIEKRMKQHQKKNETFERKEVSKDEAR
                     AIFHDNPYKLELIDGFPEGETITTYQQGDFLDLCRGPHVASTREIGPFKLQSVAGAYW
                     RGDEKRPMLQRIYATAWFTREELDDYLHKLEEAARRDHRKLGR"
     gene            complement(933..2210)
                     /locus_tag="VFQ54_07540"
     CDS             complement(933..2210)
                     /locus_tag="VFQ54_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU0164883.1"
                     /translation="MRTSATFPNRRPCLRRAARFLAAVVLFAFPFFQIVATTSAQDVP
                     QLEIDSKEFLVMNADTGEVFAQKDAHKRVPIASLTKIFTAVEAIELAPLDTEITTNES
                     DMQPSDATVMGFSPGETYTLKDLLYGMLLPSGNDAAHAIARGLGYQKGDTDDQAVQRF
                     MVLINQRIKDMGLKDTHLVNPHGYGVPGHYSSAWDVATFMNYAMTFPLLTQIMGTASY
                     TTANGLITVTQGNKMLNTYSDWVAGKTGYDDTAGWCLSNLAEQGNVRMIAVTLDGIAP
                     DDWYDDNTVLLNYAFEQQQTLTASKKAFTGDTVSYTDPAAAEIARSAKPGTDLSSLAA
                     APVSQPQTSDVKPIVPPVDTASQTLPDVAPAAIDDAPSRSFQQKIEDSARDIAAIGLA
                     ALLVLVPTLVTYRLRWGRGNRSASRVDTTTASH"
     gene            complement(2420..2496)
                     /locus_tag="VFQ54_07545"
     tRNA            complement(2420..2496)
                     /locus_tag="VFQ54_07545"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2460..2462),aa:Val,seq:gac)
     gene            complement(2552..3040)
                     /locus_tag="VFQ54_07550"
     CDS             complement(2552..3040)
                     /locus_tag="VFQ54_07550"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEU0164884.1"
                     /translation="MSHKSIYLASPLGFADSTRGFMADLVALLSAHVDVVNPWEKGVF
                     DAEFASLATENDWSRRVARLHEIDLELGRLNTASIDGVDGVFAVLDGVDVDSGTAAEI
                     GYAFAKGKYVAGLRTDFRLAGDNDGSIVNLQVEYFIEASGGRIVRTADALAGLAREYA
                     GR"
     gene            3143..3310
                     /locus_tag="VFQ54_07555"
     CDS             3143..3310
                     /locus_tag="VFQ54_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164885.1"
                     /translation="MRGALWIALVVTLIVLVFIKFATGIVWTGAFGIALLTFCISFAI
                     AIRSGRGIRRN"
     gene            complement(3313..3753)
                     /locus_tag="VFQ54_07560"
     CDS             complement(3313..3753)
                     /locus_tag="VFQ54_07560"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEU0164886.1"
                     /translation="MSTSTPSQTNVSPKSRPRSFEIVFDGGSLGNPGKGYGSYEITSG
                     GEVVRHEREDYGNNVTNNQAEYLTLIRALEWLDTLLEGRRESATVLINGDSKLVINQV
                     QGLWKINNAQLRPLAERARELLKGYAKSSLVWHARANSVKRLGH"
BASE COUNT          676 a         1132 c         1275 g          768 t
ORIGIN      
        1 tcacggccga gcttgcgatg atcgcgccgg gcggcttcct ccagcttgtg caggtagtcg
       61 tcgagctctt cccgcgtgaa ccacgccgtc gcgtagatcc gctgcagcat cggtcgcttc
      121 tcatcgccac gccagtaggc acccgccaca ctctgcagct tgaagggacc gatctcccgc
      181 gtgctcgcga cgtggggacc gcggcagaga tcgaggaagt cgccctgctg gtaggtcgtg
      241 atcgtctcgc cttcggggaa gccatcgatc agttcgagct tgtagggatt gtcgtggaag
      301 atcgcgcggg cttcgtcctt ggagacttcc ttgcgttcga acgtctcgtt cttcttctga
      361 tgctgcttca tgcgcttctc gatctcgacg aggtcgtcgg gggtgagggc gcgggggaga
      421 tcgaagtcgt agtagaagcc gttgtcgata gcgggaccga tgccgaatct ggcgtcgggg
      481 aagagttcct ggacggcttc ggccatgagg tgggcggcgg agtgacgcat gcgggcgagc
      541 tcgtaggcgc gatcgtcgcc gggttcgacg gcgatatcca tgttctcgac gtcttgtgta
      601 gttgcagcca tctgaatcct ccatgaggga agcgccgcgt catgcgacgg acagaaaacg
      661 gcccggcgat ccgcaaggga cgccgggccg acgtggttcc accctcattc cggatcttcc
      721 cgtggaggac gagatgatcc gcttgattgg cccggtatcg gaggcaaccc ggagacgatt
      781 aatggcgacg gtcagccgcg ttcttcgttc agctcacagg gggtggggaa tcggcgttca
      841 agatccggct cgcacctgtc ccggattcgc tgccatgtcg tgtcgacgac catatcctgc
      901 tcgtagcatg ctcttcactt gtccgccaaa gcttagtgac tggccgtggt cgtgtcaacg
      961 cgcgaggcag agcgattccc gcgcccccag cggagacgat acgtgacgag cgtcgggacg
     1021 agcacgagca gggccgcgag tccgatcgcg gcgatatcac gagcggagtc ctcgatcttc
     1081 tgctggaagg aacgggaggg ggcgtcgtcg atcgccgccg gcgccacatc gggcagcgtc
     1141 tgcgaagcgg tatccacagg ggggacgatc ggtttcacgt cgctcgtctg cggctgcgaa
     1201 accggagcgg cggcgagact cgagaggtcg gtaccgggct tcgcgctacg tgcgatctcg
     1261 gccgccgcgg gatcggtata ggagacggtg tcgccggtga acgccttctt cgacgccgtc
     1321 agcgtctgct gctgctcgaa ggcatagttg aggagcaccg tgttgtcgtc gtaccagtcg
     1381 tcaggtgcga taccgtcgag tgtcaccgcg atcatccgga cgttgccctg ttcggcgaga
     1441 ttggaaagac accagccggc ggtgtcatcg taaccggtct ttcccgccac ccagtcggag
     1501 taggtgttga gcatcttgtt gccctgggtg accgtaatca ggccattggc ggtcgtgtag
     1561 ctcgcggttc ccatgatctg cgtcagcagc gggaaggtca tcgcgtagtt catgaaggtg
     1621 gcgacatccc atgccgagga gtagtggccc gggacgccgt aaccgtgcgg gttcacgaga
     1681 tgcgtgtcct tgagacccat atccttgatc cgctggttga tcaggaccat gaagcgttgc
     1741 acggcctggt cgtcggtatc gcccttctgg tagccgagac cgcgcgcgat cgcgtgtgcg
     1801 gcgtcgttac ccgaggggag gagcatgccg tagaggagat ccttgagcgt gtaggtttcg
     1861 cccgggctga atcccatcac ggtggcgtcc gacggctgca tgtcggattc gttcgtcgtg
     1921 atctcggtgt cgagtggagc gagttcgatc gcctcgaccg ccgtgaagat cttggtcagg
     1981 cttgcgatcg ggacgcgctt gtgggcgtcc ttctgggcga agacctcgcc ggtgtcggcg
     2041 ttcatcacca ggaattcctt ggaatcgatc tccagttggg gaacgtcctg ggcggaggtg
     2101 gtagcgacga tctggaagaa ggggaacgcg aagagaacga cggcggcgag gaatcgcgcg
     2161 gcgcggcgca ggcaagggcg tcgattcggg aacgtggctg acgtccgcat ctgctctctc
     2221 gatagcggcc actgactgcc ctcatgtcga gcgcaaggac cgtacgttca ctcttggcga
     2281 aggatcgccc ctcaatggga acagtaccac ttcggtaaac gcgagggact ccgtcgtggt
     2341 tctcgctcca cgcccctcgg tgcggcgtgc atgcaaaagc ccgatcatca gggcgtgatg
     2401 tcgggcgatt cagggattct ggtgggcgat actggactcg aaccagtgac ctccacgatg
     2461 tcaacgtggc gctctaacca actgagctaa tcgcccttgg gcacgcgcta gtatatgagg
     2521 cgcggcccgg agaatcaagg cgaattgcct gtcagcgtcc cgcgtattcg cgagccagtc
     2581 cggccagcgc gtcggccgtg cggacgatcc tcccccccga agcctcgatg aagtattcga
     2641 cctggagatt gacgatcgag ccgtcgttat cccccgccag ccggaagtcg gtgcggagtc
     2701 cggcgacgta cttccccttg gcgaaggcat agccgatctc ggcggcggtg ccggagtcga
     2761 catcgacgcc gtcgagcaca gcgaagacgc cgtcgacacc gtcgatgctc gcggtattga
     2821 ggcgcccgag ctcgagatcg atttcgtgaa ggcgagcgac gcgccgggac cagtcattct
     2881 cggttgccag cgacgcgaac tccgcgtcga agacgccctt ctcccacgga ttgacgacgt
     2941 caacgtgggc cgagagaagc gccacgaggt cggccatgaa gccgcgcgtc gaatcggcaa
     3001 agccaagggg tgacgcgaga tagatcgatt tgtgggacac atttcctcga ttcgctgaag
     3061 gacgccggcg gggcttagga cctctatact accaaccatg aatcaacgac ggcgaccgac
     3121 cagatcaacc gaagacgcga cgttgcgggg agcgctctgg atcgcgctcg tcgtgacgct
     3181 gatcgtcctc gtcttcatca agttcgcgac gggaatcgtc tggactggtg cgttcgggat
     3241 cgcgttgctg accttctgca tcagcttcgc gatcgcgatc cgatcgggac gcggaatccg
     3301 acgcaactag gatcagtgcc cgagtcgctt cacggagttg gcgcgggcgt gccagacgag
     3361 tgacgacttg gcatagccct tgagaagctc acgggcacgc tcggcgagcg ggcgaagctg
     3421 ggcgttgttg atcttccaca atccctgcac ctggttgatc accagcttgc tgtccccgtt
     3481 gatcaggacc gttgccgatt cccggcgtcc ttcgagcagt gtgtcgagcc attcgagtgc
     3541 gcggatcagc gtcagatact cggcctggtt gttggtgacg ttgttcccat agtcctcgcg
     3601 ctcgtggcgg acgacttctc ctcccgaggt gatctcgtag ctgccatacc ccttgccggg
     3661 attacccagc gaacccccat cgaagacgat ctcgaacgat cgcggcctcg acttcggaga
     3721 gacgtttgtc tggctgggtg tcgatgtact catgattgct ccctgacgat cgaaacaaga
     3781 gacgagccgg ggcgttgctc cggctcgtct gccttcaggg tagcaaatcc ggacccatcc
     3841 ggtcaggagg c
//
LOCUS       DASTMD010000516         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1208787, whole genome shotgun sequence.
ACCESSION   DASTMD010000516 DASTMD010000000
VERSION     DASTMD010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208787"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFQ54_07565"
     CDS             <1..329
                     /locus_tag="VFQ54_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164887.1"
                     /translation="HFRAPLFGTFPMPPTLPGVFGQRIFGGEKRITHPGDALFRTLQK
                     LMGDRGPTVAEVRTAVDDIDADAVDVICTKIQQLYASRSMPRGANPWRVAQNELESAR
                     ALWSRS"
     gene            326..1531
                     /locus_tag="VFQ54_07570"
     CDS             326..1531
                     /locus_tag="VFQ54_07570"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEU0164888.1"
                     /translation="MSGTIRIAHLSDTHLGYRGLTKTDPVTGRNQRTIDIETAFTRTI
                     DHILTQDVDAVIHSGDAFHHTRPTWHSLRHFIRQMRRLEDAGIPTLVIAGNHDTPRMR
                     TGGSAYSVLELALPKIRFVADYEAIEVTEAPFDRLDLHIHAIPHGALTNPDPVIPRLQ
                     RGKRNILVCHGMVPGILAPGHHAEQGEQQLDTALLDSQFDYIALGHYHVPSNPAPKAW
                     YSGATERMGFGDWDVTPGYNVVEVHAPSEKVDVTHLPGEARPMTSLKAVYGNGRPARA
                     IADDILEQLSKDDFVTAMVRVELRETERPIRRETDSILRREYQEFAWSLTLAQERTIF
                     VTESADQPDGIDGVPDLQSLFATFVQERTGTHFDQVFATTFLERGSRALSDAILTQEA
                     PAPEEEGAA"
     gene            1528..>3093
                     /locus_tag="VFQ54_07575"
     CDS             1528..>3093
                     /locus_tag="VFQ54_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0164889.1"
                     /translation="MILSRVEIENYKQFADRHEIEIPTTATIGVIGNNGVGKTTLFEA
                     IEWCLYNPAQIRSAEIRPRGRSGFTKVAVTLDEPDSGRRFIIERELRRSSVTATIYEV
                     EESGNEVVIVQGTKPVTEYVSNRLIGLSHRAFVATFFTRQKELSFFGDMGDSDRRREV
                     SRLLGMETIRLAQQSIADDRKALAADAQSYRRQYDEQSLGRDFDAEITSARTTIASGN
                     AALIEADRKVEAATGEVKQRELELTAIEASRDRDAGFANQLADLGAQRAAARKRITEI
                     AGQIEYLDARAREREHHVAIAARLPELTAELATLETDRERFLRRRELVQASQETGRRR
                     GDLLMSIATTVDGVRHDGRVSGWSWSTDDSDAPAAAIQRLQATIASLDVDAAIAHEQR
                     LVAARESARVLDDASSKLRAFRDRLHQLNEELNVLLVEGEPTVALQELDLRQNAIQRE
                     LSDIQASRSHQETQSRQARGLIENLTHAHFGDRCPTCARPFSEYDAGIVVDSLKAQVA
                     TFTEAIRTGKSRET"
BASE COUNT          583 a         1085 c          955 g          470 t
ORIGIN      
        1 cccacttccg cgcgccgctc ttcggcacct tcccgatgcc gccgacgctt cccggcgtct
       61 ttggccagcg catctttggc ggcgagaagc ggatcaccca tccgggcgac gccctcttcc
      121 gcacgctgca gaagctgatg ggcgatcgcg gtccgaccgt cgccgaggtg cgaaccgccg
      181 tcgacgatat cgatgccgac gccgtcgacg tgatctgcac gaagatccag cagctctatg
      241 cgtcgcgatc gatgccacgc ggggcgaatc cctggcgcgt cgcccagaat gaactggaaa
      301 gcgcccgtgc gctctggagt cgatcatgag cggcacgatt cgcatcgccc atctctcgga
      361 tacccacctc ggatatcgcg gcctgaccaa gaccgatccc gttaccggtc gcaatcagcg
      421 cacgatcgat atcgaaactg ccttcacgcg caccatcgat cacatcctga cgcaggacgt
      481 cgatgccgtc attcacagcg gcgacgcctt ccaccacacg cggccaacct ggcactcgct
      541 gcgccacttc atccgccaga tgcgccgcct ggaagacgcc ggcatcccca ccctcgtgat
      601 cgcgggcaac cacgacacgc cacggatgcg caccggcggc tcggcctaca gcgtcctcga
      661 actcgcactg ccgaagattc gcttcgtcgc cgactacgag gcgatcgagg tgaccgaggc
      721 gccgttcgat cgtctcgatc tccacatcca cgcgatcccg cacggcgccc tgaccaatcc
      781 ggaccccgtg attccccgct tgcagcgcgg aaagcggaac atcctggtat gccacgggat
      841 ggttcccggc atcctggccc cggggcacca cgccgaacag ggagagcagc agctcgatac
      901 cgcgctgctc gattcccagt tcgactacat cgcgctcggt cactaccacg tgccgtcgaa
      961 ccccgcgccg aaggcctggt attcgggtgc gaccgaacgc atgggattcg gtgactggga
     1021 cgtgacgccg gggtacaacg tggtcgaggt gcacgctccc agtgaaaagg tcgatgtcac
     1081 ccacctcccg ggtgaggcac gcccgatgac gtcgctgaag gccgtctacg gcaacggtcg
     1141 tccggcgcgc gcgatcgccg acgacatcct cgagcagctc tcgaaggacg acttcgtcac
     1201 agcaatggtc cgcgtcgagt tgcgtgagac cgagcggccg atccgccgcg agaccgactc
     1261 gatcctgcgg cgtgagtacc aggagttcgc ctggtcgctc acgctggccc aggagcgcac
     1321 gatcttcgtc accgaatccg ccgaccagcc ggacggcatc gacggcgttc ccgacctcca
     1381 gtcgctcttc gcgaccttcg tccaggaacg aacggggacc cacttcgacc aggtcttcgc
     1441 cacgacgttc ctcgagcgcg gcagccgggc gctttccgac gcgatcctga cccaggaagc
     1501 gccggctccg gaagaagagg gcgccgcatg atcctcagcc gcgtcgaaat cgagaactac
     1561 aagcagttcg ccgatcgcca cgagatcgag atcccgacga cggcgacgat cggcgtcatc
     1621 ggcaacaacg gcgtgggcaa gaccacgctc ttcgaagcga tcgagtggtg cctctacaac
     1681 ccggcgcaga tccggagcgc ggagatccgc ccgcggggaa ggagcggctt taccaaggtc
     1741 gcggtcacgc tcgatgagcc ggacagcggt cgacggttca tcatcgagcg cgaactccgg
     1801 cgaagctcgg tcaccgccac gatctatgag gtcgaggaga gcgggaacga agtcgtgatc
     1861 gtccagggga cgaagccggt cacggagtac gtcagcaacc ggctgatcgg tctcagtcat
     1921 cgcgccttcg tcgccacctt cttcacacgc cagaaggaac tctcgttctt cggcgacatg
     1981 ggtgattccg atcgccggcg cgaggtcagc cgcctgctcg ggatggagac gattcgtctc
     2041 gcccaacagt cgatcgcgga cgaccgcaag gcgctcgcgg cggacgcgca gtcctaccgg
     2101 cgtcaatacg acgaacagtc gctgggccgc gacttcgatg ccgagatcac cagcgcccgt
     2161 acgacgatcg cctctggtaa tgccgcgctc atcgaggcgg atcgaaaggt cgaggccgct
     2221 accggcgagg tgaagcagcg ggaactggag ttgacggcga tcgaggcgtc tcgcgaccgg
     2281 gatgccgggt tcgccaacca gctcgccgat ctcggcgccc agcgcgcggc ggcgcgcaag
     2341 cggatcaccg agatcgccgg gcagatcgaa tacctcgatg cccgcgcgcg cgagcgcgaa
     2401 catcatgttg cgatcgcggc gcgccttccg gagctcacgg cggaactcgc gaccctggaa
     2461 accgaccgcg agcgattcct gcgacgacgg gagctcgtcc aggcgagtca ggaaactgga
     2521 cggcggcgcg gcgatctcct gatgtcgatc gcaacgacgg tcgacggcgt acgtcacgac
     2581 gggcgggtct ccggatggtc ctggtcgacc gacgattcgg atgcccctgc ggccgcgatt
     2641 caacgcctcc aggcgaccat cgcgtcgctc gacgtcgacg cagcgatcgc gcacgaacaa
     2701 cgcctcgtcg cggcccgcga atccgcccgc gtgctcgacg atgcctccag caagctccgt
     2761 gcgttccgcg accgcctgca tcaactcaac gaggaactga atgtcctcct tgtcgagggt
     2821 gaacccaccg tcgccctgca ggagctcgat ctccgccaga acgcgatcca gcgagaactc
     2881 agcgacatcc aggcaagtcg cagtcaccag gagacccaat cgcggcaggc acggggcctg
     2941 atcgagaatc tcacgcatgc acacttcggc gatcgctgcc ctacctgcgc gcggcccttc
     3001 agcgagtacg acgccggcat cgtcgtcgac tcgctgaaag cgcaggtcgc cacgttcacg
     3061 gaggcgattc gcaccggtaa gtcccgcgag acg
//
LOCUS       DASTMD010000517         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_585030, whole genome shotgun sequence.
ACCESSION   DASTMD010000517 DASTMD010000000
VERSION     DASTMD010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585030"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..893)
                     /locus_tag="VFQ54_07580"
     CDS             complement(189..893)
                     /locus_tag="VFQ54_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEU0164890.1"
                     /translation="MSWRRGPSRDREAEPRPEGPRAGRISQITAQVHDRDRVSIFLDD
                     VFAFGLAADLVLEEGLAPGMALDEAAVERLLAADEIRRATAAALNLLAYRPRSEGEIT
                     TRLRQRNFSPTAIAAAIVRLREWHYVDDADFANRWIENRQQHRPRSARMLAQELKAKG
                     VDQEIVRESIAEAGLDEVADARAVAERQRMKYASLDEETQNRRLMAFLARRGYGFDVI
                     RRALEVDETDPENEEE"
     gene            complement(897..1955)
                     /locus_tag="VFQ54_07585"
     CDS             complement(897..1955)
                     /locus_tag="VFQ54_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU0164891.1"
                     /translation="MNDSRSAATLLDALLAPPLEQALSEMEGNAPLLPRMARYHLGMV
                     DATGAPTDLETRKLVQGKRMRPLLAVLSCLAVGGRAGDAAPIAAAIELLHNFTLVHDD
                     IQDRSPNRRHRATVWRIWGDAQAINAGDGIFSAAHLAVLQTPHDMLPAESLFTLTAEF
                     NRMTIAIVRGQVLDLGFEGRGDVTPDDYLGMISGKTAAIVRYAAWAGSIVGGAPEEVS
                     DRFARMGLALGIGFQIQDDLLGIWGTSEETGKEVADDVRRRKQSLPVLLLRSQANAED
                     TELLDRLYANDEIDEPGIARVLQLLDAYDIQRQVVAKVEEAHDEASQILAEVAGGTSN
                     PGLDGIQELITRMRVRLS"
     gene            2291..2998
                     /locus_tag="VFQ54_07590"
     CDS             2291..2998
                     /locus_tag="VFQ54_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEU0164892.1"
                     /translation="MFFFDPLYIVFMLPGLLFMLWAQSKVKGNYQKYSQVRNQANVTG
                     AQAARRVLDSQGLSDVQIEPIAGELSDHYDPRTRVLRLSQGVYGVPSIAAIGIAAHEA
                     GHAIQHAKAYQPLKWRTALVPAVNIGSNIGFGILFLGIIIARPELAWVGVALFGLSTF
                     FALLTLPVEFDATRRAKAALVQIGLVDSGVAGGEESRGVARVLDAAAWTYVAGFASSL
                     LTLLYYVMMVSGMNRRN"
     gene            complement(3082..>3440)
                     /locus_tag="VFQ54_07595"
     CDS             complement(3082..>3440)
                     /locus_tag="VFQ54_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0164893.1"
                     /translation="IQRPIPIWIGASAEVAVRRAARLTDGFFANGSLDDAMDHQLAAL
                     YDELARNGRSLDDFGLEARISLASASPDDIRRSFAAWQAKGISHVSFVTMNAGFATVG
                     DHLRALEAAYRAIEGL"
BASE COUNT          586 a         1057 c         1155 g          642 t
ORIGIN      
        1 tccagcgcca gactgacccc tgcgatttct cgttcagctg cgtcattccc cagggaggcg
       61 accagacggg tcgccgggat gggaatggga tcacgtcatc ctgaaggcgc atcctgccaa
      121 cgtaatgggt gatggccagg atcgtccggt tgcacatcag agtggagtca cttcgatcct
      181 gacgggcatt attcctcctc gttttcgggg tcggtctcgt cgacttcgag tgcacggcgg
      241 atgacgtcga agccgtatcc gcggcgggcg agaaaggcca tcaggcggcg gttctgcgtc
      301 tcctcgtcga gtgacgcgta cttcatccgt tgtcgctcgg caaccgcgcg ggcgtcggcg
      361 acttcgtcga gcccggcttc cgcgatcgac tcgcggacga tctcctgatc cacgcccttc
      421 gccttcagct cctgggccag catgcgcgcg gagcggggac gatgctgctg ccggttctcg
      481 atccaccggt tggcgaagtc ggcatcgtcg acataatgcc actcgcggag ccggacgatc
      541 gcggcagcta tcgccgttgg cgagaagttc cgctggcgaa gcctggtcgt gatctccccc
      601 tcggagcggg gacgataggc gagcaggttg agggcggcgg ccgtcgcgcg acgaatctcg
      661 tcagcggcga ggagacgctc gaccgccgcc tcgtcgagcg ccatccccgg cgcgagtcct
      721 tcttcgagga cgagatcggc ggcgagcccg aaggcgaaga cgtcatcgag gaagatgctg
      781 acgcgatcgc gatcgtggac ctgcgcggtg atctggctga tacgaccggc acgcggtcct
      841 tccgggcgcg gctcggcctc ccgatcgcgt gagggaccgc gtcgccacga catcgatcag
      901 gacaaccgga cccgcatgcg ggtgatcagt tcctggattc cgtcgagacc cgggttcgat
      961 gtgccgccgg caacctccgc gagaatctgg ctcgcctcgt cgtgcgcctc ctcgaccttc
     1021 gcgacaacct ggcgttggat gtcgtaggcg tcgagcagtt gcagcacgcg ggcgatcccg
     1081 ggctcgtcga tctcatcgtt cgcatagagg cgatcgagca gttcggtgtc ttccgcgttt
     1141 gcctgcgagc ggaggagaag gaccgggaga ctctgtttgc gccggcggac gtcgtcggca
     1201 acttccttgc cggtctcctc gctggttccc cagatgccga gcaggtcgtc ctggatctgg
     1261 aagccgatgc cgagcgcgag ccccatccgg gcaaagcgat cggagacctc ctccggcgcg
     1321 ccaccgacga tcgaccccgc ccaggcggcg tagcggacga tcgcggcggt cttgcccgag
     1381 atcatgccga ggtagtcgtc cggggtgaca tcgccacggc cctcgaaacc gagatcgaga
     1441 acctgaccgc ggacgatcgc gatcgtcatc cggttgaact ccgcggtcag cgtgaagagc
     1501 gactcggccg ggagcatgtc atgcggtgtc tggagcacgg cgaggtgagc cgccgagaaa
     1561 atgccatcgc cggcgttgat cgcctgggca tcgccccaga tccgccagac cgtcgcccga
     1621 tggcggcggt tgggactgcg gtcctggata tcgtcgtgga cgagcgtgaa gttatgaagc
     1681 agctcgattg cggcggcgat cggtgctgca tctccggcac gaccgccaac ggcgagacag
     1741 ctgaggacgg cgaggagcgg acgcatccgc ttgccctgca cgagcttgcg tgtctcgagg
     1801 tcggtcgggg cgcccgtggc gtcaaccatg ccgaggtgat agcgcgccat ccggggaagg
     1861 aggggagcgt tcccttccat ctcggaaagc gcctgctcga gcggcggcgc gaggagcgca
     1921 tcgagcaatg tggctgcgga gcgggaatca ttcatggacg cgggctcgat tccggatggg
     1981 tcatcaacgc gggatgactg gatcactgat gtactcccca cctaggcgat gatcgcgtca
     2041 ccggcggccc ctaagcgagt gggcaggtga cgaagtgagc atacttcctt agagttgaga
     2101 tgtcgccgca tttgtatcag gcggatgcgg ataacggtga tgattgaacg cacgaaacga
     2161 gccgaaacgg ccttttgcac gtatacttgt gtacatgagt gagaccatag ctcaccaacg
     2221 agttgcttgc cgtcggagcc accgatttcg tcttgtggca gcaagtagcc gtagatgagg
     2281 acccacctcg atgttcttct tcgatccact ctatatcgtg ttcatgctcc cgggcctgct
     2341 gttcatgctc tgggcacaga gcaaggtcaa gggcaattat cagaagtatt cccaggttcg
     2401 caaccaggcg aatgtgacgg gagcgcaggc tgctcgccgc gttctcgata gccagggact
     2461 ctcggacgtc cagatcgagc cgattgccgg cgagctgtcg gatcactacg atccgcggac
     2521 ccgcgtgctg cggctgtcgc agggcgttta tggggttccg tcgattgctg cgatcggtat
     2581 cgcggcgcat gaggctgggc atgcgattca gcatgcgaag gcatatcagc cgctcaagtg
     2641 gcgtaccgcg ttggtgccgg cggtgaacat cggatccaac atcggattcg gcattctctt
     2701 cctcggtatc atcattgccc gccccgaact ggcgtgggtc ggtgtcgccc tcttcggtct
     2761 ttcgaccttc ttcgcgctcc tgacccttcc ggtcgagttc gacgctacgc gacgcgcaaa
     2821 ggcggcgctg gtgcagatcg ggctggttga ctcgggtgtc gccggtggcg aagagagccg
     2881 cggcgttgcc cgcgtcctcg acgccgcagc gtggacgtac gtggcgggat tcgcctcctc
     2941 gctcctgacg ctgctctact acgtgatgat ggtttccggc atgaaccgcc gcaactagaa
     3001 tcacgatcgg ccaccgtcaa gatgtcgatc tgaaaagcaa cgccccgtac ggatttccgg
     3061 acggggcgtt gacgtgcctg cctacaaccc ttcgatcgcg cggtatgccg cctcgagcgc
     3121 ccggaggtga tcgccgacgg tggcgaagcc ggcgttcatc gtgacgaacg agacgtggga
     3181 gattcccttc gcctgccacg ccgcgaacga gcggcgaata tcgtccggtg aggctgaagc
     3241 gagcgagatt cgcgcctcga gcccaaaatc atcgagcgag cgcccgttgc gggcaagctc
     3301 gtcgtagagg gccgcgagtt ggtgatccat cgcgtcgtcg agcgatccgt tcgcgaagaa
     3361 gccgtccgtt aggcgggcag cgcggcggac cgcgacctcg gcgctggcgc cgatccagat
     3421 cgggatgggg cgctggatcg
//
LOCUS       DASTMD010000518         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_941644, whole genome shotgun sequence.
ACCESSION   DASTMD010000518 DASTMD010000000
VERSION     DASTMD010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941644"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1681
                     /locus_tag="VFQ54_07600"
     CDS             35..1681
                     /locus_tag="VFQ54_07600"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU0164894.1"
                     /translation="MGQSADRNNTPANGSGGAPAGDGGASAERAFWERHGSRFTSENL
                     TEDGPDASGPAANRSEPDRGPAQPDFLGRFRAKMNQLADYIDENTTFNDPKRDLKRDA
                     RERSSERRGKDIFTNFVNPDMASSRTAITHPDFLWARLREIPGVKSWKILNDSLFLAL
                     SFVLGLLWFVVGIVMFSLGVGLLIVWVGIPILAATFALMIWGAQLERSRIKMFMGVEI
                     PTPYAYLPKEGKPWKRAWGFVRNPQLWRDVTYLFLLFPIGIFELAVLSVPLTFLTSPL
                     IFVFGGSHSVYFWSIGSFGESLLAFIFGLVVLVPFSMLINFVALMHVEFGRWLLAPTT
                     EEVLTERVEELTESRSAVMRAMHMERRRIERDLHDGAQQRLVNLAMELGLAREKMGTD
                     LDATRKLLEESHEEAKMVLTELRELVRGIHPAVLTDRGLDAAISAIAGRSPIPVGVDI
                     NLKERHPDEVEGTAYFVVVESLTNIAKHSGATEANVSIRKAGSWLRIVVSDNGRGGAD
                     PTKGTGLRGLQDRIAALDGRFAIDSPVGVGTSILVEIPCA"
     gene            1672..2349
                     /locus_tag="VFQ54_07605"
     CDS             1672..2349
                     /locus_tag="VFQ54_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0164895.1"
                     /translation="MRVTIAEDSVLLREGLIRLLTEHDAEIVAAVADGDALITAIEKE
                     RPEIIICDVRMPPTFRDEGLRAAIQIRRRWPNTPILVFSQYVEERYAAELLEGGTRGI
                     GYLLKDRVADVREFIVAVRRVASGGTVLDPEVVAQLVTRRRRDERLSQLTQREQEVLA
                     LMAEGRTNNAIADALFVSEGTVEKHIRGIFIKLGLEDAESVHRRVLAVLTFLRLGEDD
                     LGQPRAF"
BASE COUNT          440 a          834 c          808 g          459 t
ORIGIN      
        1 ccgaattgcg cgcacccagc aaggaccgga cagaatgggc caatcagcag atcgcaacaa
       61 cacccccgcc aacggatccg gcggcgctcc agccggcgac ggcggagcct ccgccgagcg
      121 cgcgttctgg gaacgccacg gctcgcggtt tacctccgag aacctcaccg aggatggacc
      181 ggacgcgtcg ggcccggcgg cgaaccgatc ggagccggat cgtgggccgg cgcagccgga
      241 tttcctcggc cggttccgcg cgaagatgaa ccagctcgcg gactacatcg acgagaacac
      301 gaccttcaac gatccgaagc gggatctcaa gcgtgacgcc cgcgaacgat ccagcgaacg
      361 tcgcggcaag gacatcttca cgaacttcgt gaacccggac atggcatctt cccggacggc
      421 gatcacccat cccgatttcc tgtgggcgcg actccgcgag attcccggcg tcaagagctg
      481 gaagattctc aacgacagcc tcttccttgc cctgagcttc gtcctcggcc tgctctggtt
      541 cgttgtcggg atcgtgatgt tctcgctcgg cgtcggcctc ctgatcgtgt gggtgggaat
      601 cccgatcctg gcggcgacgt tcgccttgat gatctggggc gcccagctcg agcggagccg
      661 gatcaagatg ttcatggggg tcgagatccc gacgccctac gcctatctgc ccaaggaggg
      721 caagccgtgg aaacgggcgt ggggattcgt ccgtaacccg cagctctggc gtgatgtcac
      781 ctacctcttt ctcctcttcc cgatcggcat cttcgagctg gccgtcctca gcgtcccgct
      841 caccttcctg acgtcaccgt tgatctttgt cttcggtggg agtcacagcg tctacttctg
      901 gtcgatcggc agcttcggcg aatcgctcct cgcgttcatc ttcggactgg tcgtgctcgt
      961 gccgttcagc atgctgatca acttcgtcgc cctgatgcat gtcgagttcg gccgctggct
     1021 cctggcgccg acgaccgagg aagtcctcac cgagcgcgtc gaggaactga cggagagtcg
     1081 ttccgccgtg atgcgggcga tgcatatgga gcgtcgccgg atcgagcgcg atctccacga
     1141 cggcgcgcag cagcggctcg tcaacctcgc gatggagctc ggactggctc gcgaaaagat
     1201 gggcaccgat ctcgatgcca cacgaaagct gctcgaggaa tcgcacgagg aggcgaagat
     1261 ggtccttacc gaattgcgcg aactcgtgcg cgggattcac ccggcagtcc tgaccgatcg
     1321 tggtctcgac gcggcgatct cggcgatcgc tggccgctcg ccgatcccag tcggcgtcga
     1381 catcaatctg aaggagcgcc atccggatga agtcgagggg acggcctatt tcgtcgtcgt
     1441 cgagtcgctg acgaacatcg ccaagcacag tggtgcgacg gaggcgaacg tcagcatccg
     1501 gaaggcaggg tcgtggctgc ggatcgtcgt gtcggataat ggacggggtg gggccgatcc
     1561 caccaagggt accggactcc gtggtctcca ggatcgcatc gccgcgctcg atggtcgctt
     1621 cgcgatcgac agcccggtcg gagtcggcac gagcatactg gtggagattc catgcgcgta
     1681 accatcgcgg aagattccgt cctgcttcgt gaggggctga ttcgcctcct gaccgagcac
     1741 gacgccgaga tcgtggctgc cgtcgccgat ggtgatgccc tcatcaccgc aatcgaaaag
     1801 gagcggcccg agatcatcat ctgcgacgtg cggatgccgc caaccttccg cgacgagggg
     1861 ttgcgggccg cgatccagat ccggcgccgg tggccgaaca caccgatcct cgtcttctcg
     1921 cagtacgtcg aggaacggta cgcggcggaa ctgctcgaag gtggcacgcg cggcatcggc
     1981 tacctgctca aggatcgcgt cgccgacgtt cgcgagttca tcgtcgccgt ccgccgggtg
     2041 gcgtctgggg gcaccgtcct cgacccggaa gtcgtcgccc agctggtgac ccggcgtcgt
     2101 cgcgacgagc gtctctccca actcacgcaa cgcgagcagg aagtcctcgc gctgatggcg
     2161 gaaggccgga cgaacaacgc gatcgccgac gcgctcttcg tgagcgaggg gacggtcgag
     2221 aagcacatcc gcggcatctt catcaagctc ggtttggagg atgccgaatc ggtccatcgc
     2281 cgcgttctcg cggtccttac cttcctccgc cttggcgagg acgacctggg ccagccccgc
     2341 gcgttctgac cgcgcacccc gataccactt ccgctcccgt aggggcgagc ctcgtgctcg
     2401 cccgggccga catcgccgac gaatgtcttg cgtaagctcc tggacattcc gcccgttggg
     2461 ggatcacagg ttccgtctcc agcaacctca agccccgtca ccaacccggt gacagggctt
     2521 cagtgatttg gcgtgggagt a
//
LOCUS       DASTMD010000519         3760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1656998, whole genome shotgun sequence.
ACCESSION   DASTMD010000519 DASTMD010000000
VERSION     DASTMD010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656998"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(637..2949)
                     /locus_tag="VFQ54_07610"
     CDS             complement(637..2949)
                     /locus_tag="VFQ54_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU0164896.1"
                     /translation="MAGPTPTYRPSHPYAARRRPVRWPWTVASLIMFAIIGMTALLVG
                     DRLRAPDQISQEAGITPESDKVAQVVETASPAPTDVPTVTPIPTPTSVSPEGPEKTAE
                     NWLVAWQKGDYAAMYTMLTTTSQKSITQADFVARYQGIVDKASLTKVKASIMGQPGLD
                     GKVPYQITFTSALVGDISEKNSIPVVQEGSRWAISWTPSLIFRDLGTDGCIDFEGTTP
                     TRGKILDRNGKVLAEDQPFAQVGVDVGEVVDPTTYDELSPVIGMSASDIRDLVQGKPA
                     NWRVPLKNMPADKSTDLLNAVKGIPGATVTSTTARTYPYKALTAQVVGWVSPATEDDI
                     KNDTTGTIQDNQMIGRTGIEAGADDLLTGKPGGQLEVVNCDSRAVEKVLAARDPTPSS
                     DIYLTLDVAFQKSVDQALTAQVKGDQRSSAVILDPQTGAVLAMVSHPSFDPNGYVTGV
                     FSTADQRVLNDDLLTASVNRASYELYPTGSIFKVITTSAAMHYLKYTADTQINCPSSF
                     SIGDQTWDDWVVDYGEPPQGELTLHEGLVQSCNTVFYQIGQKLDDTNSEDLPAMAKAY
                     GLGSLTGIKYLPELAGTIPDPAWKEKNVGDGWSTGDAVNMAIGQGYVLATPLQMANVY
                     ATIANGGHLLTPYIVDRTKKQGDTQTVQVGKRVERGKIPLTADQMGQLQDMLKDQTSN
                     DNNEGSAQVFLGFDFDISGKTGTAENTLDGSGEPHSWFAGYGPGRDKAPTIASCVMFE
                     NKGEGVSFAAPATKAIYTAYEKSSLAKKEP"
     gene            complement(3459..>3760)
                     /locus_tag="VFQ54_07615"
     CDS             complement(3459..>3760)
                     /locus_tag="VFQ54_07615"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEU0164897.1"
                     /translation="SFPKITFVSTSVQAKSSDEFKVTGDLTLHGVTQPIVLDVEHTGR
                     GKSPFGFEVAGFSATTKISRKAFGLEWNAALETGGVLVGDEIKISIEIEAVPAES"
BASE COUNT          637 a         1159 c         1244 g          720 t
ORIGIN      
        1 cccgggttca tcgcctgtga ggacagggaa caccggatcg actgccggcc agtggcgact
       61 ggcatggatc catgagaagc aaaaggcgca tgaatacacc ttcattgtct cgcatgatac
      121 ctgatcccaa ccgcgttgtc acccggtcac cgggtggcct ctccaacacc tctccatgca
      181 cgtcctccct cggtcacgtc accaccccag tcacgtcctc cctcggtcac gtcctcccga
      241 gcctgtcgag ggatcggttg gcatccccgc gcttcccgaa cagcggcgac tgcgtagggg
      301 cgagcctggt gctcgccctc cgccgcaggc ggactccaac ggaagcatcc acctcaagac
      361 gcgttccgca acggcgacaa gcgccgtggg gacgggaatg cggcgatcgt ccagggtggg
      421 catgtggtga ccagccgatc cctcgacaag ctcgggaaga cgtgaatggg gtgatgaagg
      481 gggacggtgt gaatgggaac gacgtgcggg gaggatggat ggcgtgatcg ggatctggcg
      541 accaggatga ggggacggcg gggccgttcg gacagcaaga cgggcgggca tggtgcccgc
      601 ccgtctgaag acgaatgaac tcatgggtca ggacggtcag ggttccttct tggcaagcga
      661 ggatttctcg taggccgtgt agatggcctt cgtcgccggc gccgcgaagc tgacgccctc
      721 acccttgttc tcgaacatca cgcaggacgc gatggtcggg gccttgtccc gacccgggcc
      781 atagccggca aaccaggaat gcggctcgcc actcccatcg agcgtgttct ccgccgttcc
      841 cgtcttgccg gaaatgtcga agtcgaagcc gaggaagacc tgcgcagatc cctcgttgtt
      901 gtcgttgctc gtctgatcct tcagcatgtc ctgcaactgg cccatctggt cggcggtcag
      961 cggaatcttg ccccgttcga cccgcttccc gacctgcacc gtctgcgtgt caccctgctt
     1021 cttcgtcctg tcgacgatat acggcgtcag gagatgccca ccattggcga tcgtcgcgta
     1081 aacgttggcc atctggagcg gcgtcgccag gacgtatccc tgtccgatcg ccatgttcac
     1141 cgcatcaccg gtcgaccagc catcgccgac gttcttttcc ttccacgccg gatcggggat
     1201 cgtcccggcg agttcgggca ggtatttgat gccggtcagc gatccgaggc cgtaggcctt
     1261 cgccatggcc ggcagatcct ccgaattcgt atcgtccagc ttctgcccga tctggtagaa
     1321 gaccgtgttg cacgactgaa ccagcccctc gtgcagtgtc aattcacctt gaggcggttc
     1381 tccatagtcg acgacccagt cgtcccaggt ctggtcgcca atggagaagc tcgacggaca
     1441 gttgatctgg gtgtcagccg tgtacttgag atagtgcatc gcggccgagg tcgtgatgac
     1501 cttgaagatc gagccggtcg ggtagagctc gtacgaggcg cgattgacgc tcgccgtgag
     1561 gagatcgtcg ttgagcacac gctgatcggc cgtgctgaag acaccggtga cgtatccatt
     1621 cggatcgaag ctcgggtggc tgaccatcgc gaggacggct ccggtctgcg ggtcgagaat
     1681 gacagcggaa gaacgctggt cgcccttcac ctgcgcggtc aacgcctggt cgaccgactt
     1741 ctggaaggcg acatcgagcg tcaggtagat atcggacgag ggagtgggat cgcgcgccgc
     1801 gagcaccttt tcgacagccc ggctgtcgca gttgacgacc tcgagctgac cacccggctt
     1861 cccggtgagg aggtcgtcgg cgccagcctc gatcccggtg cgaccgatca tctggttgtc
     1921 ctggatcgtg ccggtcgtgt cgttcttgat gtcgtcttcc gtcgccgggc tgacccagcc
     1981 gacgacctgc gccgtgagcg ccttgtatgg atacgttcgc gccgtcgtcg acgttaccgt
     2041 cgcgccgggg attcccttga cggcgttgag aagatcggtc gacttgtcgg ccggcatgtt
     2101 cttgagcggc acgcgccagt tcgccggctt cccctggacg agatcgcgaa tgtcgctcgc
     2161 ggacatcccg atgaccgggc tcaactcgtc gtacgtcgtc ggatccacga cctcgccgac
     2221 atcgacgcca acctgcgcga acggttggtc ctcggcgagg accttgccgt tgcgatcgag
     2281 aatcttgcca cgcgtcgggg tcgtgccctc gaagtcgatg cagccgtcgg tcccgagatc
     2341 cctgaagatc agggaaggag tccaggagat ggcccaacgc gacccttcct ggacgacggg
     2401 aatcgagttc ttttcgctga tgtcaccgac gagggcggag gtgaaggtga tctggtaggg
     2461 cactttcccg tcgaggccgg gctgacccat gatgctcgcc ttgaccttgg tgagggaggc
     2521 cttgtcgacg attccctggt accgcgccac gaaatcggcc tgggtgatgg atttctgact
     2581 ggttgtcgtc agcatcgtgt acatcgccgc gtagtcgccc ttctgccagg cgaccagcca
     2641 gttctccgcg gtcttttccg gtccttcggg gctgacgctg gtcggcgtcg ggatcggggt
     2701 gacggtaggc acgtcggtcg gcgccggcga ggcggtctcc acgacctggg cgaccttgtc
     2761 gctctccggc gtgatgccgg cctcctgcga gatctggtcg ggcgcgcgca ggcggtcccc
     2821 gacgagcagg gcggtcatgc cgatgatggc gaacatgatc agtgaggcga cagtccacgg
     2881 ccagcgaacg ggccgccgtc gcgccgcgta gggatgggaa ggacggtagg tgggagtcgg
     2941 tcctgccaag gaggatgccc tttcggagat cggcgaggat gagccggtcg ggccagttag
     3001 gtgccgggac cggacgtggg cgacggtctg atcgggtagt tgtcaataaa ccaggatgat
     3061 cgcacagatc ggcgcgatgc gccgccctgc atacgacgcg gtcagtatgc actgtccgta
     3121 catgccgtgg caaaaggact ctggtggcga atgcgctgcc cggcatgcgg gcggagttcc
     3181 gctgggggag ccggacgacg cgacagccgt gcgtcgtccg gctcccggaa tcgaggcatc
     3241 atgcagcagc gatgcgctcg tcatcttgta cgcgtggcgc gggacggcgg tttcgcggtt
     3301 acgtcaagcg tgctcgagca tccctcgcac aaaagaagaa gggcggacca gaaatctggt
     3361 ccgcccttct tcgatcatcg atcggcgatt ggtccgggag cgacgtcggc gcgcaaaccg
     3421 gtcatcccaa aaccattgct ggcgatgagc ggcgctgact agctctccgc cggaaccgcc
     3481 tcgatctcga tcgagatctt gatctcatcg ccgacgagga caccgcccgt ctccagcgcg
     3541 gcattccact cgaggccgaa cgccttgcgg ctgatcttcg tggtggccga gaagccggcg
     3601 acctcgaagc cgaacgggga cttgccgcgg ccggtgtgct cgacatcgag cacgatcggc
     3661 tgggtgacgc cgtgaagcgt caggtcgccg gtgaccttga actcgtcgct gctcttcgcc
     3721 tgcaccgagg tgctgacgaa ggtgatcttc gggaagctgg
//
LOCUS       DASTMD010000520         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_673356, whole genome shotgun sequence.
ACCESSION   DASTMD010000520 DASTMD010000000
VERSION     DASTMD010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673356"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VFQ54_07620"
     CDS             complement(<1..1092)
                     /locus_tag="VFQ54_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU0164898.1"
                     /translation="MCGVFGFAGTEHEVAATVGPALQSLEYRGYDSWGVSWLVEREME
                     TRKHTGRLVLDELPAIRASAAVGHTRWATHGGVTDANAHPHLSQSGRFSVVHNGIIEN
                     AQQLKNLLLAGCRFQSATDSEVVPHLIEREVSHGTPVREAVELVFSLLEGHNAIVVMD
                     AESGVIHAISSGSPLLVAEGDVGAYVASDVIAFQDLATTMMPLPDLRLVRLSVDGVEL
                     LDAGTHTWRHPRMTPIVRRPTDKLGSFDHFTQKEIAEQPDVIERVLHDNASTERLAAA
                     IADSATVILTGSGSAYFAARFGAAWITRHAGKPALAIPASEFGELSPFTTTDALVLAL
                     TQSGETADVIDAVQDGLRSGARVQAVVNVP"
     gene            1639..>2704
                     /gene="purB"
                     /locus_tag="VFQ54_07625"
     CDS             1639..>2704
                     /gene="purB"
                     /locus_tag="VFQ54_07625"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU0164899.1"
                     /translation="MIERYTRPEMGAIWSDKAKVEHWLAVELAVCEAWGRRGEIPVDD
                     LTAIRGASIDLERMREIEKETDHDVIAFLRAVGETVGPASRFIHLGLTSSDVVDTGLG
                     IQAKQAGELLLRELKRLTGIVGERAIEHKRTITIGRSHGMHAEPTTFGLKLAVWYDEL
                     RRHQKRLEHAIDDIAVGKISGAVGTHAHVPWGLEDEVCAELGLGIEFASTQIVQRDRH
                     AFFLSVLAGIGATLEKFGVEIRHLQRSEVGEAEEFFGEGNQGSSAMPHKRNPHESERV
                     SGLARLLRGYATPGLENVALWHERDISHSSVERVIFPDACIVLDYMLDLTSELIESWV
                     IYPGRMIANLESSGGLIYSQR"
BASE COUNT          492 a          850 c          882 g          480 t
ORIGIN      
        1 ggggacgttg acgaccgcct gcaccctcgc gccgcttcgc agcccgtcct gcaccgcatc
       61 gatcacatcc gccgtttccc cgctctgcgt cagggcgagc acgagcgcgt ccgtcgtcgt
      121 gaatggtgag agctcgccaa actccgaagc aggaatggcg agcgccggct tgcccgcatg
      181 ccgtgtgatc caggcggcgc cgaaacgcgc ggcgaaatat gcagagccgg atccggtcag
      241 gatcaccgtc gcgctgtccg cgatggccgc cgcgagccgc tcggtcgatg cgttgtcgtg
      301 aaggacgcgc tcgatgacgt ccggctgttc cgcgatctcc ttctgcgtga aatggtcgaa
      361 cgagccgagc ttgtccgtgg ggcgacggac gatcggcgtc atcctcgggt gccgccacgt
      421 gtgggtgcca gcatcgagca gctcgacgcc gtcgaccgag aggcggacca atcggaggtc
      481 cggcaacggc atcatcgtcg tggcgagatc ctggaaggcg atgacgtccg acgcgacgta
      541 cgcgccgaca tctccctcgg cgacgaggag cggcgacccc gagctgatgg cgtgaatgac
      601 gcctgactcg gcatccatca cgacgatcgc gttgtgtccc tcaagcagcg agaagaccag
      661 ttcgacggcc tccctgacag gcgtaccgtg ggagacctcc cgctcgatca gatggggcac
      721 gacctcggaa tcggtcgccg actggaaacg acatcccgcg agaaggaggt ttttcagttg
      781 ctgggcgttc tcgatgatcc cgttgtggac gaccgagaat cggccacttt gggagaggtg
      841 ggggtgcgcg ttggcgtcgg tcacgccacc gtgcgtcgcc cagcgcgtgt gaccgaccgc
      901 ggcggacgcg cgaatggcgg gaagctcatc gagcaccagc cggccagtat gttttcgtgt
      961 ttccatctcg cgttcgacga gccagctcac gccccaggag tcgtagccgc gatattcgag
     1021 cgattgcagc gccggtccca ccgtcgccgc gacttcgtgt tccgtaccgg caaagccgaa
     1081 gactccgcac atcggatagc ccctcccgac accgacgcaa ggatcaccga gatccctgtt
     1141 tgcgggcaaa cgcaaaccag gaactcgatc gagccgttgg tcgagcatcg tgtaacgaat
     1201 gaattgttgg gagggagagg gaagcacatg gacgacgatt tgtcgggttg tcaccaatcc
     1261 ggtttgacgt cgcgctgacg actatgcctg ccggggaacc atccgccgag tctttcgatc
     1321 ggttcccgcc tcgtcaactc gcgtacccgg gcgagtcctg gcgctgtaag gagaatacga
     1381 aattcctcct ccccgccgag attctagcgc acccgttctg ccctgaacaa ccgccgacgg
     1441 atgagggact ctcgtacgta cgcgtcgatg cttcgccctc ttcgtgtcga actggcaccg
     1501 cggccggtcg cttcgtccat cttcgccgcg aaagggacgt cgagtgaagc aagaccttat
     1561 acttgcaagg gtatttagag gatcggttcc ctcagcgttc gcgggcaatc cggtctagtc
     1621 ctgaaaggga tcgggttcgt gatcgaacgg tatacgcggc cagagatggg cgcgatctgg
     1681 agtgacaagg cgaaggtcga gcactggctc gccgtcgaac tcgcggtctg cgaggcgtgg
     1741 ggccgccgcg gcgagattcc cgtggatgat ctcacggcca ttcgcggcgc gagcatcgat
     1801 ctcgagcgga tgcgcgagat cgagaaggaa accgaccacg acgtgatcgc cttccttcgc
     1861 gccgtcggcg agacggtggg accggcgtcg cgtttcatcc atctcggcct cacctcatcc
     1921 gatgtcgtgg acaccggact cgggatccag gcgaagcaag ccggcgagct cctcttgcgg
     1981 gagctgaagc ggctcaccgg gatcgtcggt gaacgtgcga tcgaacacaa gcggacgatc
     2041 acgatcggac gcagtcatgg catgcacgcc gagccgacca cgttcggcct gaagcttgcc
     2101 gtctggtacg acgagcttcg ccgccatcag aagcggctgg agcacgcgat cgacgacatc
     2161 gcggtcggca agatctctgg cgcggtcggg acgcatgcac acgtgccgtg ggggcttgag
     2221 gacgaggtct gtgccgagct cggcctcggg atcgagttcg cctcgaccca gatcgtccag
     2281 cgcgatcgac atgccttctt cctcagcgtg cttgccggga tcggcgccac gctcgagaag
     2341 ttcggggtgg agatccgtca tctccagcgg agcgaggtcg gcgaggcgga ggaattcttc
     2401 ggcgaaggca accagggctc atcggcgatg ccgcacaagc ggaatccgca cgaatccgag
     2461 cgcgtcagcg ggctcgcccg cctgcttcgt ggctacgcga cgccggggct cgagaacgta
     2521 gcgctctggc atgagcgcga catcagccat agctcggtcg agcgggtgat cttcccggac
     2581 gcctgcatcg ttctcgacta tatgctcgat ctcacatctg aactgatcga gagctgggtg
     2641 atctaccccg gacgcatgat cgccaatctg gaatcctccg gtgggctcat ctactcgcag
     2701 cgcg
//
LOCUS       DASTMD010000521         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1744943, whole genome shotgun sequence.
ACCESSION   DASTMD010000521 DASTMD010000000
VERSION     DASTMD010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744943"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            605..677
                     /locus_tag="VFQ54_07630"
     tRNA            605..677
                     /locus_tag="VFQ54_07630"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:638..640,aa:Arg,seq:acg)
     gene            complement(725..1894)
                     /locus_tag="VFQ54_07635"
     CDS             complement(725..1894)
                     /locus_tag="VFQ54_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164900.1"
                     /translation="MLDLLVFGAAVVAASFALAWAAEAAQKDISGALAIAILALIAVL
                     PEYAVDLYYAFRSGSDAGYEQFAAANMTGSNRLLLGLGWPLVVFVALFVARKIAKAGG
                     ESGRSLPSILRLPGNSRLDIGVLAIVSVLAFAIPLMGMIALPFGILLIVIFAAYLWRA
                     GRVEEDDGEEEFVGAAALIANMPQRARRITVITMFVAAAAIILLSAEPFADALVQSGR
                     ALGVDSFFLVQWLAPLASEAPEFIVAVLFAVRGHGVAAIGTLIASKINQWTLLVGSLP
                     IAHALGGGGLNLELDPRQVEEFVLTASQTVMGVAILIALRFHRWSAFALIALFAVQFA
                     VTDTAGRYVLSAIHLVIAVVALAINRRDILPTITAPFRRPVTLAKHPALIDKQAG"
BASE COUNT          355 a          683 c          806 g          363 t
ORIGIN      
        1 gctccagctg cgccacgtgg atcgccttcg tgcgggcgat gccgctgacg tcgcaatact
       61 cgaagcggat gagcttcacg ccggcctcgt gggcggcctc gacgacttct tcgatgttca
      121 cagggcttcc tcgtgtgcga gacccatgag ctccgtgatg acgcgctttc ggtcggcggt
      181 gatcacgagg tggctccctg cctgtgtcgc ggagatggtg agcggatacc tccttggtat
      241 atcagccgta tttcagccgc accgacgtgg ggttacggga gtattacgcg cgagcactga
      301 cggtggcccc gtcggctccg cgggcgggcc ggtgggatac tggagccatg gcaacagttg
      361 ttctcctcac ggtgttctgg gtactggtcg gtgtcggtgc ggtcatcggc gtcgcggcgc
      421 tcgtggcgat gatcaagagc ccgtactcct gatccgggcg tcgttccgcg ggtggacagc
      481 gccgcgtcgc acgaactgcg cgtcgcgggc actgatccat cccgcgacgg cccaccggcc
      541 ggattcgcca ggcggcgatc cgctggtata gtcgtcgggt tgggaactgc ggttccgaac
      601 agatgcgccc gtagctcagc ggatagagca attgactacg gatcaatagg ccgggggttc
      661 aaatccctcc gggcgcacac cttcagcccc gtctcaggac ggggctgttt tgcgtccgga
      721 gccgtcatcc ggcctgtttg tcgatgagcg cgggatgctt cgcgagcgtg acggggcgac
      781 ggaatggcgc ggtgatcgtc gggaggatgt cacgccggtt gatcgcgagc gccaccacgg
      841 cgatgacgag gtggatggcg ctgagcacat agcggccggc ggtgtcggtg acggcgaact
      901 gcacggcgaa cagtgcgatg agcgcgaacg cggaccagcg gtggaaccgc agtgcgatca
      961 gtatcgccac gcccatcacc gtctggctcg cggtcagcac gaactcttcg acctggcgtg
     1021 ggtcgagctc caggttcagc ccgccgccgc ccagtgcgtg ggcgatgggg agggagccga
     1081 cgagcagcgt ccactggttg atcttcgacg cgatgagcgt cccgatcgcg gcgaccccgt
     1141 gaccgcggac ggcgaacagc acggcgacga tgaactccgg cgcttccgag gcgagggggg
     1201 ccagccactg cacgaggaag aagctgtcca cgccgagcgc ccggcccgac tgcacgagcg
     1261 cgtccgcgaa cggttcggcg gagagcagga tgatggcggc cgccgccacg aacatggtga
     1321 tgacggtgat ccgcctggcg cgctgcggca tgttcgcgat cagtgcggcg gccccgacga
     1381 actcctcctc gccgtcgtcc tcctcgacgc ggcccgcacg ccacaggtat gccgcgaaga
     1441 tcacgatgag gaggatgccg aacggcaggg cgatcatgcc catgaggggg atggcgaacg
     1501 cgagcacgga cacgatggcc agcacgccga tgtccagcct gctgttgccg ggcaggcgaa
     1561 ggatgctcgg caggctcctg cccgactcgc ctccggcctt cgcgatcttg cgggcgacga
     1621 acagcgcgac gaagacgacg aggggccatc cgagaccgag caggaggcga ttcgatccgg
     1681 tcatgttcgc ggccgcgaat tgctcgtagc cggcgtcgga tccggaacgg aaggcgtagt
     1741 agaggtccac ggcgtattcg ggcagcacgg cgatcagggc gaggatcgcg atcgcgaggg
     1801 ctccggagat gtccttctgc gcggcttccg ccgcccacgc gagagcgaat gacgcggcca
     1861 cgacggcggc cccgaagacg aggaggtcga gcacggggtt cggtgcgaac cccgtgacgc
     1921 ggaaggcgat cgccggggag gcgatcgcga ggcagaggat gaggcgtcgg atgagggcgg
     1981 agggcatggc tgctgtcctt cggttgcaac cggccggagg catggtggat cgcttcggtc
     2041 ggtctgtgtc ggccgaaggt ctcactcgcc cagatctggg tggtacgccg ggcggatcgt
     2101 cgtgatcgcc agtgtgtcga cgcacctctc gacccgcgct gcgggcccgg agctactccc
     2161 cttcgtcctc gatcgttgca gtgcgcgggg ctcgtgtcca tgtgtcc
//
LOCUS       DASTMD010000522         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2817412, whole genome shotgun sequence.
ACCESSION   DASTMD010000522 DASTMD010000000
VERSION     DASTMD010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2817412"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFQ54_07640"
     CDS             complement(<1..335)
                     /locus_tag="VFQ54_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164901.1"
                     /translation="MARNNIPPDGRTSSVPEVPRPTGVIWEELAAEGVVHESQVLIVG
                     LGADLPARLILTRERLALVNDGEIAIEVPRTWLRPSPKLAAENGVRIYMTPTGPGNGG
                     DTETLSIRAR"
     gene            complement(844..2346)
                     /locus_tag="VFQ54_07645"
     CDS             complement(844..2346)
                     /locus_tag="VFQ54_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0164902.1"
                     /translation="MASRSGEITLRDLLAWEPQLQLLVRPTSGPAGDDPLANDVEWVV
                     TARASAPMLPSLRGGELVLLPQRVVAESGVPFTMLLHELAGRPVAGVLTDIRVDLTTD
                     VPLPLLSVPVVTTDLETEINRLLTSRRGDLLRTGADLEHLISSMISRNARPSELIDAI
                     GRRLHLGITIATESGTIIFTTLDHSKIQLPVNVSPSTTTRVGDQSWIPHPLRGDRVLW
                     VWPIAPAQGALSRLVVRQLVDGIQRSFDQDDATAPRGAARTRVLNELLLQAPTDPNTI
                     EAAALRAGLPIGAQFRVALHPADIPEEPIYRRLTSLGTVHDAGIIDGFTARIVTSQIA
                     RNPNIPAKLAPGAPRMWLALSAPMTSPRQLPDATRQARFIAGLADRELLTGAELRFDD
                     EAVLGAYALLFDRWGSASLGRYIDQLIGNLLREDRRGLLRETLRVYLEHGGAQRSTSD
                     RLAIHRNTLTYRLRQIREVLHLDPDDPNSRLGLHLAILAAELPPAPPVHG"
     gene            2743..>2959
                     /locus_tag="VFQ54_07650"
     CDS             2743..>2959
                     /locus_tag="VFQ54_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164903.1"
                     /translation="MNSPFDVASARPDRTASWRRILSGSTPILIVASLVGLIGFLSPL
                     VLPLAQRADADTSAHAGDAPILFALVTA"
BASE COUNT          539 a          892 c         1019 g          509 t
ORIGIN      
        1 cgcgcgcgga tcgacagcgt ttccgtatcc ccgccgttgc cgggtccggt cggtgtcatg
       61 tagatgcgga caccgttctc ggcggcgagc ttcggtgatg gccgcaacca tgtccgcggc
      121 acctcgatcg cgatttcacc atcgttgacc agcgccaggc gttccctggt caggatcagg
      181 cgggcaggaa gatcggcgcc gagtccgacg atgaggacct ggctctcgtg gacgactccc
      241 tccgccgcga gttcttccca gatgacgcct gtcggtcgcg ggacctcggg cactgatgat
      301 gttcgcccat cgggcgggat gttgttgcga gccactctgt ggacctccct cgctggccgg
      361 ggcgtgccgc atccgcaatc gggtggacat tcgccatcgg tgacagccgg gctaacgtgt
      421 cgtgggcagg tgcgctgcgg aactccacca gaacgcatgc accatcatcg ggaatgacgc
      481 caaccagacc gaccggtgaa cccagaccaa cgcaggcacc gtctgcggcg ccggcgctgg
      541 cgatgagtga gattgcggga aggctgcggg gaagcctgca gggtgcgtcc ggttcgtgtt
      601 gacacacaca tatccgacgg accgcaacgg agcctcggaa gaggccctgc cgcggtccgt
      661 ttcaatcgaa acggttcgaa cggtgagcac cggattctgc ccggagatgt ctcaaacgga
      721 catcaaccag ccaaggcaga ggtgtacgta cactctatca tagttgtcgg aaaatggaaa
      781 acaagggagg cgctgttgtt gcggggcggt tcgcctggtg ttgcgaaaga cgccccggca
      841 atcttatccg tggacgggag gagccggcgg aagctccgcg gcgaggatgg cgaggtggag
      901 acccaggcgc gagttcggat cgtccggatc gagatggagc acctcgcgga tctgacgcag
      961 gcggtaggtc agcgtgttgc ggtgaatcgc gaggcgatcc gaggtcgagc gctgggcgcc
     1021 gccgtgctcg agatagacgc gcagcgtctc gcgcagcaat cctcgccggt cctcgcgaag
     1081 gagattcccg atcagctggt cgatgtagcg accgagcgag gcgctacccc accgatcgaa
     1141 gagtagcgca tacgcgccga gcaccgcctc gtcgtcgaag cgaagttccg ccccggtcag
     1201 gagctcgcgg tcggcgagac cggcgatgaa gcgcgcctga cgcgtcgcat ccggaagctg
     1261 acggggagat gtcatcggcg ccgagagcgc gagccacatc ctgggcgcac ccggtgcgag
     1321 cttcgccggg atgtttggat tgcgcgcgat ctggctcgtg acgatgcgcg cggtgaagcc
     1381 gtcgatgatc cccgcatcat ggacggtgcc cagcgatgtc aggcgacgat agatcggttc
     1441 ctcggggatg tccgcgggat gaagcgccac gcgaaactgg gcgccgatcg gcagcccggc
     1501 gcggagtgcc gccgcctcga tcgtgttcgg atcggtcggg gcctgcagga gcagctcgtt
     1561 gaggacgcgg gtgcgggcag cgccccgtgg cgccgtcgcg tcatcctggt cgaacgagcg
     1621 ctggatgcca tcgacgagct ggcggacgac gagacgggag agcgcgccct gggcgggcgc
     1681 gatcggccag acccaaagca cccgatcgcc gcgcaacggg tgagggatcc aggactggtc
     1741 ccctacgcgt gtcgtcgtcg acggcgagac attgacggga agctggatct tcgaatgatc
     1801 gagcgtcgtg aagatgatcg tcccgctctc ggtcgcgatc gtgatcccga ggtgcaagcg
     1861 ccgtccgatc gcgtcgatga gctcggaggg gcgggcattg cgggagatca tcgaggagat
     1921 caggtgctcg agatcggcgc cggtacggag cagatcgccg cgacgggaag tcagcaggcg
     1981 attgatctcg gtctcgaggt cggtggtgac gacggggacg gagaggagcg gcagcgggac
     2041 atccgtcgtc aggtcgacgc gaatatcggt gaggacaccg gcgacggggc gcccggcgag
     2101 ctcgtggagc agcatcgtga acggaacgcc ggattcggcg accacgcgct gcggaagcag
     2161 gacgagttct cccccgcgca gggatggcag catcggtgcg ctggcgcgtg ccgtgacgac
     2221 ccattccaca tcgttggcca gcggatcatc tcccgccggg ccggaagtgg gacgcacgag
     2281 caattgcagt tgtggttccc aggcgagaag atcgcgaagg gtgatttcgc cgctccgtga
     2341 tgccatattc gctttatcct tcgtcttggt ggaaagtgtg gaaggcatcg ttgccggttg
     2401 aatccacgac ggaagatcgg gctgccgggt ggttccgcat gggaatttcc atgcacgaaa
     2461 ttcacccacg agctggcaac tcccacagcg ccactcatgc atgatgccca atttcgctcc
     2521 ggacgcgacg atgaatccgg actccatgca gatgctacca aagaagtggc acgatgcact
     2581 agccagtatc cgggaggagt ttcgggtgtt ttatgcaacg tgcacgaccg ctaccccggc
     2641 gtattggtct gattctctac atagaggcag gaattcccga ctaatcacgt cacaatcgcg
     2701 ggtggacgcc attctggcca attgcacaag tcgaagttcg tgatgaactc cccattcgat
     2761 gtggcgagcg ctcgtccgga tcggaccgcc agttggcgcc gaatcctgtc cggttcgaca
     2821 ccgatcctga tcgttgccag cctggttgga ctcatcggct tcctctcgcc cctcgtgctt
     2881 ccgttggcgc agcgggccga cgccgacacc agcgcacacg ccggcgacgc accgattctc
     2941 ttcgcgctcg tcacggcct
//
LOCUS       DASTMD010000523         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2012579, whole genome shotgun sequence.
ACCESSION   DASTMD010000523 DASTMD010000000
VERSION     DASTMD010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012579"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..2593)
                     /locus_tag="VFQ54_07655"
     CDS             complement(62..2593)
                     /locus_tag="VFQ54_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164904.1"
                     /translation="MNATLTQMKHSARNLVAVSLAITIGVAFVAATFQFGSVLQQTIW
                     NSVAAPVAGSDIIITADGGRLDGTTLTRVNGVDGVATAEGRQFVGLNLKAGTRQTTSF
                     TTSVPTTEAIRDRISLTDGRLPAKPGELVVVKDMLKQFDVRLGSTVSVTPLGGDGAGT
                     DAATPVTIVGVMSPVAFRAAGAPDMYAWPSDLAKWLPTSAYVSIMVTTDPGTRTSDVI
                     ARLNAVLGGDAVVRSYQDYAEAQANHAVGSADVITTGLLAFAFVVLFVAGIVIGNTFT
                     ILVTQRVRQLALLRCIGATKAQVRRSVLTEAFVLALIASVIGVALGLAVVAGGVRLYG
                     AIAGTSVIATSAPVPATAYLVPPVLGIVATLIAAWMPARSATRVAPLDALRPTGAPVL
                     GSRTSRARIIWTLLLLGFGCLLLAGGVVLATNAIGPGPLAVGVGGGIISALGVLIGAV
                     LIVPRTVRLIGALAGRLGAPGAIALSNIGRNPRRTTATAVALLIAVTLVTMMSVGTAS
                     MKTTLGKVIDERDPIDIVVSYAPTTSVDPNQSGLPDATLTTVTQTLQASGKIAQTMPV
                     LNVTAWLEGMPLSANGVDPATALTITHAPDQLTGLADGTAIVPEDVATAYGIVDGDTL
                     SLTVPSSGAIDVTAHVTSYPGDALIMTTHDATVLDPPAIPSQVWASFTSGTNAGRAVG
                     SIQDSLANVGGISFSGGAESKQSNDRILDTILLIVTALLGVAVIIALVGVGNTLSLSI
                     LERTRESAILRAMGLTRRQLRAMLAIEGVLISVVGAVLGIVLGTAYGLIGAVTLLGDR
                     WGISLALPGDRIAEIVVVAIVAGLLASVLPARKALKVSPVAALAE"
     gene            complement(2595..>3228)
                     /locus_tag="VFQ54_07660"
     CDS             complement(2595..>3228)
                     /locus_tag="VFQ54_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0164905.1"
                     /translation="EGRFTAIMGPSGSGKSTLMHCLAGLDTVSSGKVFLGGTDLTALD
                     DTALTILRRQRIGFVFQSFNLLPAFTAEQNIMMPLTLAGIRKPDRHWFDTLVQTLGIG
                     DRLKHRPSELSGGQQQRIAIARALLTRPDVVFADEPTGNLDSRSGSQVLEFLRGSTRD
                     LGQTVVMVTHDPHAAAYADRVIFIADGELAGQLDQPTPDSVLNALRALGE"
BASE COUNT          573 a         1057 c         1105 g          493 t
ORIGIN      
        1 ccgcctctcg cctacccatg acgatcgccc caacgagacg ctcatgcgaa cattggacgc
       61 gctattccgc cagcgccgcg acgggtgaga ccttgagcgc cttccgcgcc ggcagcaccg
      121 aggcgagcaa cccggcgacg atcgcgacga cgacgatctc cgcaatccga tcgccaggca
      181 gcgccagcga aatcccccac ctgtcgccaa gcaacgtcac ggccccgatc agtccgtagg
      241 ccgttccaag gacgatcccg agtaccgccc caacgaccga gatcaacacg ccttcgatcg
      301 ccagcatcgc gcgcaactgc ctgcgcgtga gacccatcgc gcgaaggatt gccgattccc
      361 gggtgcgttc gaggatcgag agcgagagcg tattcccgac gccgacgagc gcgatgatga
      421 ccgcaacgcc gagcaacgcc gtcacgatca ggaggatcgt gtcgaggatg cggtcgttcg
      481 actgcttcga ttccgcaccg ccactgaagg agataccgcc cacgttggcg agcgaatcct
      541 ggatcgatcc caccgcccgc ccggcgttgg tgccggaggt gaaactcgcc cacacctggg
      601 aggggatcgc cggtgggtcg agcacggtcg cgtcgtgcgt cgtcatgatc agggcgtcgc
      661 cgggatagct cgtgacgtgc gcggtcacgt cgatcgcccc ggaggacggg acggtcagcg
      721 acagcgtgtc cccatcgaca atcccatacg ccgtcgccac gtcctcgggg acgatcgccg
      781 tgccatcggc gaggccggtg agctgatcgg gggcgtgagt gatcgtcaac gccgtcgccg
      841 gatcgacacc gttggcggaa agcggcatcc cctcgagcca cgccgtcaca ttcagcacgg
      901 gcatcgtctg cgcgatcttg ccgctggcct gcaatgtctg ggtcaccgtc gtcagggtcg
      961 cgtcgggaag gccgctctgg ttcgggtcga cactcgtcgt cggcgcgtac gagacgacga
     1021 tgtcgatcgg atcgcgttcg tcgatcacct tgccgagcgt cgtcttcatg ctcgcggtac
     1081 caaccgacat catcgtcacc agcgtgaccg cgatcagcag ggcgacggca gtcgcggtcg
     1141 tccgccgggg gttgcgaccg atgttggaga gggcgatcgc accaggagcg ccaaggcgac
     1201 cggcgagggc gccgatcaga cgcaccgttc gcggcacgat caggacggcg ccgatgagca
     1261 caccgagcgc ggagatgatg ccgccgccga caccaaccgc cagcgggccg ggaccgatcg
     1321 cattcgtcgc gagcaccacc ccaccggcaa gcagcaggca tccgaatccg agcaggagta
     1381 gcgtccatat gatgcgcgct cgtgacgtcc gtgacccgag caccggcgca cccgttggcc
     1441 ggagcgcatc gagcggggcg acgcgcgtcg cgcttctggc cggcatccac gccgcgatca
     1501 aggtcgcgac gatgccgagc accggtggca cgaggtaggc cgtggccgga accggggcac
     1561 tggtcgcgat cacactggta ccggcgatcg caccgtagag gcggacgccg cccgcgacca
     1621 cggccagacc gagggcgacg ccgatcaccg aggcgatcag ggcgaggacg aacgcctcgg
     1681 tcaacacgga gcggcgcacc tgcgccttcg tggcgccgat gcagcgcagc aaggcgagtt
     1741 ggcgcacgcg ttgggtgacc agaatcgtga aggtgttgcc aatcacgatc ccggcgacga
     1801 agaggacgac gaacgcgaag gcgaggagac cggtggtgat cacgtcggcg ctcccgacgg
     1861 cgtggtttgc ctgtgcctcc gcatagtcct ggtagcttcg gacgaccgca tcgccgccga
     1921 gaacggcgtt caggcgggcg atcacatcgg aggtccgcgt tcccggatcg gtggtcacca
     1981 tgatcgaaac gtaggccgag gtcgggagcc acttcgcgag atcgcttggc caggcataca
     2041 tgtcgggcgc accggcggcg cggaaggcaa cgggactcat gacgccgacg atcgtcaccg
     2101 gcgtcgcggc atcagtcccg gcgccatcgc cgccgagcgg agtgacgctg acggtcgacc
     2161 cgagccggac atcgaactgc ttcagcatgt ccttgacgac cacgagttcg cccggctttg
     2221 cggggagcct gccgtccgtc agcgagatac gatcgcggat cgcctccgtc gtgggcacgc
     2281 tggtggtgaa ggacgtcgtc tggcgcgtcc cggctttcag gttgagcccg acgaactggc
     2341 gcccttccgc cgtggcgaca ccgtccaccc cgttgacacg tgtcagcgtc gtgccatcga
     2401 gccgaccgcc atccgcggtg atgatgatgt cggatcctgc caccggcgca gcgacggaat
     2461 tccagatcgt ctgttggagc accgagccga actgaaaggt cgccgcgacg aaagcgacgc
     2521 cgatggtgat ggcgagcgac acggcaacga gatttcgtgc ggagtgcttc atctgggtga
     2581 gcgtggcgtt catcctactc tcccagcgcc cggagcgcgt tgagcaccga gtccggcgtc
     2641 ggctggtcga gctggccggc aagctcacca tcggcgatga agatgacgcg atcggcgtag
     2701 gcggcggcgt ggggatcgtg cgtcaccatc acgaccgtct ggccgaggtc gcgcgtgctg
     2761 cctcgcagga actcgagcac ttgcgacccc gatctcgagt cgagattccc ggtcggttcg
     2821 tcggcgaaga cgacatctgg tcgcgtcagg agggcgcgtg cgatcgcgat gcgttgctgc
     2881 tgcccgccgg agagttcgct cggtcgatgc ttgaggcgat cgccgatccc gagtgtctgg
     2941 acgagcgtgt cgaaccagtg tcggtccggc ttgcgaatcc cggccagggt gagcggcatc
     3001 atgatgttct gctcggcggt aaaggcgggc aggagattga aggactggaa gacgaagccg
     3061 atccgttgcc gccggaggat ggtcagggcg gtgtcgtcga gcgcggtgag atcggtgccg
     3121 ccgaggaaga ccttgccgct cgagaccgtg tcgagaccgg cgaggcaatg catcagcgtc
     3181 gatttccccg atccggatgg tcccatgatc gccgtgaaac gtccctcc
//
LOCUS       DASTMD010000524         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1299300, whole genome shotgun sequence.
ACCESSION   DASTMD010000524 DASTMD010000000
VERSION     DASTMD010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299300"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFQ54_07665"
     CDS             complement(<1..270)
                     /locus_tag="VFQ54_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164906.1"
                     /translation="MTRMEQDNGMNLDAIPVIDTHTHPFPPELTAISWTDLRNGISVG
                     FRERTDSDNDTMLLSRMMLRQLGRLLDVPATPEAVIAARNERATAD"
     gene            complement(826..1152)
                     /locus_tag="VFQ54_07670"
     CDS             complement(826..1152)
                     /locus_tag="VFQ54_07670"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEU0164907.1"
                     /translation="MIALISDNLDQIVELCREYRIRKLDLFGSAATGAFDPETSDVDF
                     IADLGEYERGVSKRFFRFADALEELLGRDVDVLTEPMIKNPYFRYAVEKQRVSLYEAR
                     DREAVA"
     gene            complement(1277..>1648)
                     /locus_tag="VFQ54_07675"
     CDS             complement(1277..>1648)
                     /locus_tag="VFQ54_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEU0164908.1"
                     /translation="MKLETAKRLSDARDACVELREITADETLGGFLDNRIPKLAVWKL
                     IEIVGEALRQAEHTDRTIATRLPNIREVVDTRNRIVHGYDSVSFRVLWEIVESEIPPL
                     QGLLEDLLRDAPAIPGTYLPE"
BASE COUNT          341 a          479 c          493 g          335 t
ORIGIN      
        1 atcggcggtg gcgcgctcgt tccgggccgc gatcacggct tccggcgtgg cggggacgtc
       61 gagaaggcga ccgagctggc ggagcatcat ccggctgagg agcatcgtgt cgttgtcgga
      121 atcagtccgt tcccggaagc cgacggaaat cccgttccgg agatcggtcc atgagatcgc
      181 ggtgagttcg ggcggaaacg ggtgcgtgtg ggtgtcgatg acgggaatcg cgtcgaggtt
      241 cataccgttg tcctgctcca tcctggtcac gtgcgatcgt tgcctgggct ctttgcacaa
      301 gaccgatcca ccccgggcga tgatcgcgcc gggtggtccg tccgtgcgtt cttacgggag
      361 caggatgggg ttggcgggaa gtggtgtcaa cggcgcgatc ggggttcgaa cgtggatatg
      421 cccagatcgg attacacgcc acgatcaatg caaacggaca ggagcatctg ggtcacctgg
      481 gaacgccttc gagaactacc acgatgtctc gccctcaccg atatcagggg ccccggtcaa
      541 cagaagttcg agcctggact gcaagggagg gatctcgtcc aggacgagat cccagaggag
      601 actgtagtca atgtcgtcgt aaccatgtac gagaggattc cgcgtctcga tgatctcgcg
      661 atcgcggcaa cggcattccg atcgctcagt cccgcctgac gaagagcctc gccgatgttc
      721 tcgataagct tctgtacgat gagttgcagg ccgcggtcgg aaagaagaaa ttctctcgac
      781 ctgcccatac cgaagtcccg aacctccgcg caggcagaac gaacatcatg caaccgcttc
      841 gcggtctcga gcctcataga gactcactcg ctgtttctcg accgcatagc ggaaataggg
      901 attcttgatc attggttcgg tcaggacatc cacatcccgc ccgaggagtt cttccaaagc
      961 atcggcgaaa cgaaagaatc gcttcgacac gccacgctcg tactcaccga ggtcggcgat
     1021 gaagtcgaca tcgctcgtct ccggatcgaa ggcaccggtg gccgccgacc cgaagaggtc
     1081 gagcttgcga atccggtact cccggcagag ttccacgatc tggtccaggt tgtcggagat
     1141 gagtgcgatc atgcgtgtcg tccctgtgcc gtcgagcaat cctgagacga ttgtagcgcc
     1201 cagcacgacg cgacgactca gttcagaact tcccaaacct tacacggcct acgcagaatg
     1261 aaatttgccg atacggctat tcaggcaagt aggtaccggg aatggccggc gcgtctcgca
     1321 agaggtcctc gagtaatccc tgcagaggcg ggatctcgct ttccacgatc tcccagagaa
     1381 cgcgaaaact cacgctgtca tatccgtgaa caatgcgatt ccgcgtgtcg acgacctcac
     1441 gaatattcgg aagccttgtc gctatggtgc gatcggtatg ttccgcctgc cttagggctt
     1501 cgccaacgat ctcgatgagc ttccataccg cgagcttcgg gatccgattg tcgaggaatc
     1561 ctccgagtgt ctcgtcagca gtgatctcac gtagttcaac gcaagcatcc cgggcgtcac
     1621 taagccgctt tgcggtttcg agcttcat
//
LOCUS       DASTMD010000525         1686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1567110, whole genome shotgun sequence.
ACCESSION   DASTMD010000525 DASTMD010000000
VERSION     DASTMD010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567110"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="VFQ54_07680"
     CDS             complement(<1..1443)
                     /locus_tag="VFQ54_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164909.1"
                     /translation="MTQNGMDDRPDQENEESGGAPLAPPDPTPSSMRSRPLFRPAPYP
                     SPRANGEEPELSDRGRAYAESVERTPSEPLIPVAPITGLSEERTPPSRTELLGSRTSA
                     VVATVLRNWPFAIPIALFAVAAMIVPTMTQIATTDDWGYSRSVEILVEDGHLTVFPVV
                     AATAVGQILWGALFGEIFGLDLGVMRLSTVVAVAIGGMALFGLLRQLGVSRWRSSMGV
                     SLYLFNPLSFILSFTFMTDPHFTSWMLVAMVFYAWGLRDEETRPWAIVVGSIAAGYAF
                     WIRQQGALIPFAVILYLVVTRRLWFNWRSLRLAAQAALAPAVMLVGYYVWLKWFNDVT
                     AVQQGFFDEMKSYGWSGAWRLTRNLTYIELAYLGIILVPILLAVVPVWRSRSRSGSRV
                     SASTVTPAAGFRTPLGYWLFVAVSIVIAWGLFAFTWENRRMPYISQFVGAGGLGPPDV
                     PGSRMRLIEWPDIYTIATVAAVAGAIVASLL"
BASE COUNT          337 a          551 c          561 g          237 t
ORIGIN      
        1 cagcaggctc gcgacgatcg ctccggccac cgcggcgacc gtcgcgatcg tgtagatgtc
       61 gggccactcg atcaagcgca tccggctccc gggaacgtca ggggggccta gcccgccggc
      121 gccaacgaac tgggagatgt acggcatccg ccggttctcc cacgtgaagg caaagagtcc
      181 ccaagcgatg acgatggaaa ccgcgacgaa gagccaataa ccgagtgggg tccggaatcc
      241 ggcagccggc gtcaccgtcg acgcggaaac gcgcgagcct gaccgcgacc gcgaccgcca
      301 gacagggacg acggcgagca gaatcggcac gaggatgatg ccgagatagg cgagctcgat
      361 ataggtcagg ttccgggtga ggcgccaggc accgctccag ccgtagctct tcatctcgtc
      421 gaagaagccc tgctggaccg ccgtcacgtc gttgaaccac ttcagccaga cgtagtaccc
      481 gacgagcatc acggcgggcg ccagcgccgc ctgggcggcg agccgaaggc tccgccagtt
      541 gaaccagagt cgccgcgtca ccaccaggta gaggatcacg gcgaagggga tcagcgcccc
      601 ttgttgccgg atccagaacg catatccggc cgcgatcgat cccaccacga tcgcccaggg
      661 acgagtttcc tcgtcccgga gcccccacgc gtagaagacc atcgcgacaa gcatccacga
      721 ggtgaagtgg ggatcggtca tgaaggtgaa cgagaggatg aacgagaggg gattgaagag
      781 atagagactc acgcccatgc tgcttcgcca ccggctcacg cccagctggc gcagcagccc
      841 gaacagcgcc atcccgccga tcgccaccgc aacgaccgtg gagagccgca tcaccccgag
      901 atcgagcccg aagatctcac cgaagagcgc tccccagaga atctggccca cggctgtcgc
      961 cgcgacgacg ggaaagacgg taaggtggcc gtcttcgacg aggatctcga ccgagcggga
     1021 gtatccccag tcgtccgtcg tcgcgatctg cgtcatcgtc ggaacgatca tcgcggcaac
     1081 cgcgaagagc gcgatcggga tcgcgaacgg ccaattacgg agaaccgtcg cgacgaccgc
     1141 ggatgtccgg ctacccagca gttccgtccg gctcggggga gtcctctcct ccgatagtcc
     1201 cgtaatcgga gcgacgggaa tcagcggctc ggagggcgtc cgctcgacgg attcggcgta
     1261 ggctcgccca cgatccgaga gttccggttc ctcaccgttc gcccggggag aggggtaggg
     1321 ggcagggcgg aagagcggtc gtgacctcat ggaagatggc gtcggatccg gcggcgcgag
     1381 gggcgctccg ccggattcct cgttttcctg gtccggacga tcgtccattc cgttctgcgt
     1441 caagccggct tcccccatcg cgatcgaatc cgtgcattcg ttcggagtat gcggcaggag
     1501 cagacctccc tgcatccccg gcccagctat ccctgcgcgg ccacccagga cgccaccgcg
     1561 tgatcgacga gatgggatac ggcggaccac gagtcgaagg tgggataggg cagcatgtgg
     1621 tcgccgggaa tctcgtgata cgcccggagg cgcggcaggt cactgacgag gcggcgcgta
     1681 tcccgg
//
LOCUS       DASTMD010000526         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_136179, whole genome shotgun sequence.
ACCESSION   DASTMD010000526 DASTMD010000000
VERSION     DASTMD010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136179"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1290)
                     /locus_tag="VFQ54_07685"
     CDS             complement(229..1290)
                     /locus_tag="VFQ54_07685"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU0164910.1"
                     /translation="MNSSFDLPQHTSAYAAFEPGGPIAPWSFERRALRSHDVAVKVLF
                     CGICHSDLHAVNNPGTDFPVVPGHEMVGTIVALGSDVTSFALGDTVAIGTIVDSCHVC
                     EPCLDEKEIYCREFPTLTYNGTDRIDGTRTRGGYSDHYVADEHFVYHLPEGLDPAASA
                     PLICAGITTYSPLRYWNVGPGMTVGIVGIGGLGHLAIKFARAMGAHVVAFTTSASKVD
                     EALNLGAHEVVLSSDGAQMEAQAYRFDFILDTVSRSYPMDPFLRALKLDATLCSLGIP
                     DHFEFSPVLLAMGRRSIASSGAGGTRETKEMLEFCREHHIGADVEVIAPNEINAAFAR
                     LERGDVHYRFVIDMQRSAS"
     gene            1388..1945
                     /locus_tag="VFQ54_07690"
     CDS             1388..1945
                     /locus_tag="VFQ54_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0164911.1"
                     /translation="MVTKEERILDAAWQLIMRHGFRKVTMSDIADAAGMSRPSLYASF
                     ANKESIVDTFTSRKSDLYDIETEMQLRDRESLAGRLAAVFEIWIVEPFASVIDLDNGL
                     DLLTNSEIYAPAGTSDFYARFERHLAEILRSEFATTSSMTAEDTAHILMMATKGLKAT
                     TQTLPELRRMIDGLITMTTATARCG"
     gene            complement(2018..2308)
                     /locus_tag="VFQ54_07695"
     CDS             complement(2018..2308)
                     /locus_tag="VFQ54_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HEU0164912.1"
                     /translation="MSERSYYITFVYQLIDDYADWNNGVFTLSGQDGVLAVTPGGEPV
                     ATVTIQGLAALIWAGQDPADFIYRGWGDVDDTAAATLRALFPVAYPVLHEEF"
BASE COUNT          489 a          689 c          753 g          439 t
ORIGIN      
        1 ttggcgccaa ttcgccttcg atacgatcct tatcctcgga ttcgtcatcg atattcttta
       61 gcgctaccat gccaatcccc ttatgactcc attatcggtg agagatgtct tctctccttc
      121 gctcccatca taggggagac actacacaat tgaaagccgc cagggagagg gtcgatttgc
      181 acgtatcgac ggcctccccg gcgctagcga atggcggaac gagttgcgtt acgaggctga
      241 ccgctgcatg tcgatcacga accggtagtg gacatcgccg cgctcgagac gagcgaaggc
      301 ggcgttgatc tcgttcgggg caatcacttc cacatcggcg ccgatgtggt gctcgcggca
      361 gaactccagc atctccttcg tctcgcgggt gccgccggcg ccggaactgg cgatactgcg
      421 acgacccatc gccaggagca ctggactgaa ctcgaagtga tccggaattc cgagcgagca
      481 gagcgttgcg tccagcttca gcgcgcgcag gaacgggtcc atcggatagc tgcgggagac
      541 ggtgtcgagg atgaagtcga accggtaggc ttgagcttcc atctgggccc cgtcgctcga
      601 gagcaccacc tcgtgcgcgc cgaggttgag cgcctcatcg accttcgatg cggaggtcgt
      661 gaaggcgacg acgtgggcgc ccatcgctcg cgcgaacttg atcgccagat gaccgaggcc
      721 gccgatcccg acgatgccga ccgtcatccc cggcccgaca ttccagtagc gcagcggcga
      781 ataggtggtg atgccggcgc agatgagggg agcggatgcc gccggatcga gaccttccgg
      841 caggtggtag acgaaatgct catccgcgac atagtggtcg gaatacccgc cgcgggtgcg
      901 cgttccgtcg atccgatcgg tgccgttgta ggtcagggtg gggaactcgc ggcagtagat
      961 ttccttttcg tcgaggcagg gttcgcagac gtggcaggaa tcgacgatcg tcccgatcgc
     1021 caccgtatcg ccgagcgcga acgaggtgac gtcggagccg agggcgacga tcgtgccgac
     1081 catctcgtgt cccggcacga ccgggaaatc cgtgccagga ttgttcacgg cgtgcaggtc
     1141 ggagtggcag atgccgcaga agagaacctt caccgcaacg tcgtgagatc gaagcgcgcg
     1201 tcgctcgaac gaccacggag cgatcgggcc gcccggttcg aacgccgcgt aggcgctggt
     1261 gtgctgaggg agatcgaagg aggaattcat ggatgctccg tgtgaggtac attacaggtg
     1321 aacacgatct gtaaacccgt ttgttggtac agattaacac atatcgttag tatgtaaagg
     1381 cccgagtatg gtaaccaaag aagagaggat tctggacgcc gcctggcagc tcatcatgcg
     1441 acacggattt cgcaaggtga cgatgagcga catcgccgat gccgccggca tgtcacgacc
     1501 atccctctat gcgtcgttcg cgaacaagga atcgatcgtc gataccttca cctcgcgaaa
     1561 atccgatctc tacgacatcg agacggagat gcaactgcgc gatcgggaat cactcgcggg
     1621 ccggttggcg gcagtcttcg agatctggat cgtcgagccg ttcgcgtcgg tgatcgatct
     1681 tgataacgga ctcgatcttc tgacgaattc ggagatctat gctcccgcgg ggacaagtga
     1741 tttctatgcc cgcttcgagc ggcatctggc ggagatcctg cgctcggagt tcgcgacgac
     1801 atcgtcgatg acggcagaag acaccgcgca catcctgatg atggcgacga aggggctcaa
     1861 agcaacgacg cagacgctcc cggagcttcg ccgcatgatc gacggcctga tcacgatgac
     1921 gacggcgacg gcgcgctgcg gctgacccgc gtcgatcgtc tggccctggg ggcgatcatc
     1981 ccggtcatcg attacgcgac cgggatgatc gggttggcta gaactcctcg tggagaaccg
     2041 gataggcgac cgggaagagc gcccgcaagg tcgcagccgc cgtgtcgtcg acatcgcccc
     2101 agccgcgata gatgaagtcg gcgggatcct gtccggccca gatcaatgcg gcaaggccct
     2161 ggatcgtgac cgtcgcgacc ggttcgccac cgggcgtgac ggcgaggacg ccgtcctgac
     2221 cagacagcgt gaagacgccg ttgttccagt cggcgtagtc gtcgatcagc tggtagacga
     2281 aggtaatata atatgagcgt tcgctcagta aagaatctga tccaaacgac tcaagacggt
     2341 gacgatgtca ccgacgcctc cgatctaaga
//
LOCUS       DASTMD010000527         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_228108, whole genome shotgun sequence.
ACCESSION   DASTMD010000527 DASTMD010000000
VERSION     DASTMD010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228108"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VFQ54_07700"
     CDS             complement(<1..775)
                     /locus_tag="VFQ54_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164913.1"
                     /translation="MADESVPLSPSGKTPRDDDDAQPTESLDIDPPRPQTNADTLRSF
                     FARAASGDTTPSSEPSSSNAEPDVDDPAHDGADTAAIPHVEEDGRAATWMSSFKSDAC
                     SASTESQAGDSEDATIVTPAPTEPTPERIASESATAAGAGPAAPVNGPVPPAAEEIPP
                     PGDASTGAKANARASGDAAPRPGSRMPRFQLPKLGNLDSIWNRLTLDPVRAIVPTVPH
                     QITLAVGAVLALLSLLANSGGLALIIAGGIVPILVLLTLN"
     gene            complement(768..926)
                     /locus_tag="VFQ54_07705"
     CDS             complement(768..926)
                     /locus_tag="VFQ54_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164914.1"
                     /translation="MKTAIWQGKGGESLTILSSNRYGPRFLFATGMLVHLSTREFHAL
                     FVKEDFHG"
     gene            1330..>2117
                     /locus_tag="VFQ54_07710"
     CDS             1330..>2117
                     /locus_tag="VFQ54_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164915.1"
                     /translation="MKNESNSTEQPAAVPVADDGIASRSVRTRLSRRSVVAGALTGAA
                     GLGLAFAEAGTRGGRSASAAQEASATPEGSPAASPVPTNTPTPTPSPTPVPLLTIVTD
                     QQPTYSGPPLETTRLNLFVASAGLTTFSPPSMAQDPQIAASYLDSLVWADNNTMEPEP
                     ALATSWSWNKDGTELTIKLRQGVKWHDGTAFTAQDVSFSLNVYRLDYDSAVARFFANV
                     DHFAINDPNSIKVVFSAPDGAFVFNALTLPIFQRAQYTKFWAKNP"
BASE COUNT          359 a          635 c          711 g          412 t
ORIGIN      
        1 gattgagggt gagcaggacg aggatcggga cgatcccgcc ggcgatgatg agcgcgagtc
       61 cgccactgtt cgcgagaagc gagagcaggg ccaggaccgc gccgacggca agcgtgatct
      121 ggtggggaac ggtggggacg attgcccgca cgggatcgag cgtcagtcga ttccagatgc
      181 tgtcgaggtt gccgagtttc ggcaactgga agcgcggcat cctgctcccc ggacgcgggg
      241 cggcatctcc agacgctcgt gcattcgctt tcgcgcctgt cgaggcatct cctggtggcg
      301 gaatctcctc tgcggctggc ggcaccgggc cgttcaccgg cgcggcggga ccggccccgg
      361 cggcggtggc tgattccgat gcgattcgct ctggcgtggg ttcggttggc gccggcgtca
      421 cgatggtggc atcttcgctg tcacctgcct gggattcggt ggaggccgag caggcgtcgg
      481 acttgaacga actcatccac gtcgccgcgc gtccgtcctc ctcgacgtgg ggtatggcgg
      541 cggtgtctgc gccgtcatgc gccggatcgt cgacgtctgg ttcggcattg gaggatgaag
      601 gctcgctgga cggggtcgtg tctccggatg cggcgcgagc gaagaatgat cgcagcgtat
      661 ccgcattcgt ctgtgggcgt ggcgggtcga tgtcgaggct ctcggtcggc tgtgcgtcgt
      721 cgtcgtcgcg cggggtcttc cccgaaggtg acaagggcac tgattcatca gccatggaag
      781 tcctctttca cgaacagcgc gtggaattca cgcgtcgata gatgcacgag catgccggtg
      841 gcgaagagaa aacggggtcc gtacctgttg gaagagagta tagtcagcga ttcgccgccc
      901 ttgccttgcc agatggcagt tttcatggca aatgtcgtgc cggttccgac ggtccggagt
      961 gtatcggtgc ctggtggtca attgtcattg cgacgtaacc agatagtaca cagctgcgtt
     1021 ctctacagtg ggagtcgcta tcgattcgcg aaacgcggga tcgtggctga aacggcgcga
     1081 cggtggggag actttgtctc atacaaccaa cccatgctgc ttcatcgtat tccgttcggg
     1141 cgtcgatggt tcgtccggct ccataatggc catcttggtg gaatgatgac atcgcgggac
     1201 atgccgaacc ttaaactttc tatgtccgta cagggtgtag tatggtgtgg cgcatgtctg
     1261 gccgggcgag tgtgatgctc gcgaggccgg cggtgttcaa ttcatgggat tttgaagcat
     1321 gggtgtgatg tgaagaacga gagcaattcg accgagcaac ctgcagccgt ccccgtggcg
     1381 gacgacggca tcgcctcccg ctcggtgcgg acacgtctca gccggcgttc ggtggtcgcc
     1441 ggcgcgctga ctggcgcggc gggcctgggt ctggcgttcg ccgaggcagg aacccggggc
     1501 gggcgatccg ccagcgcggc ccaggaagca tcggcaacgc cggaaggctc ccccgcggcg
     1561 tcgccggtcc cgacgaacac gccaaccccg acaccatcgc ccacacccgt tccgctgctc
     1621 accatcgtca ccgaccagca gccgacctat tcggggccac cgctcgagac cacgcggctc
     1681 aatctcttcg tcgcaagtgc cgggctgacg acgttcagtc cgccgtcgat ggcccaggat
     1741 ccgcagatcg ccgcgagcta tctcgattcg ctggtctggg cggacaacaa tacgatggag
     1801 ccggaacccg cgctcgcgac atcgtggtcc tggaacaagg acggcaccga actgacgatc
     1861 aagcttcggc agggcgtgaa gtggcacgat ggcacggcgt tcacggcgca ggacgtctcg
     1921 ttctcgctga acgtttacag gctcgactat gacagcgcgg ttgctcgctt cttcgcgaac
     1981 gtcgaccatt tcgcgatcaa cgatcccaac tcgatcaagg tcgtcttctc cgccccggac
     2041 ggcgccttcg tcttcaacgc gctgaccttg ccgatcttcc agcgcgccca gtacacgaag
     2101 ttctgggcga agaatcc
//
LOCUS       DASTMD010000528         1734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2818201, whole genome shotgun sequence.
ACCESSION   DASTMD010000528 DASTMD010000000
VERSION     DASTMD010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2818201"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFQ54_07715"
     CDS             complement(<1..592)
                     /locus_tag="VFQ54_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0164916.1"
                     /translation="MATTTSDIIVDRLLDWGVDVVFGLPGDGINGVFEALRTRQEKIR
                     FIQVRHEEAAAFAACAYAKYTGKLGVCIATSGPGGIHLLNGLYDAKCDGAPVLALTGH
                     TYHDMIGSGYQQDIPLDRLFMDVAAYSERVSGPAHARSAIDTAIKSALTWRTVAHLTI
                     QKDIQEWPLDEKLRSEPNIAQHTSLLPHVAAAHPAMQ"
     gene            complement(753..1193)
                     /locus_tag="VFQ54_07720"
     CDS             complement(753..1193)
                     /locus_tag="VFQ54_07720"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEU0164917.1"
                     /translation="MHRLTRGALAGGAATLLMSVPIVAAEKLGFFQNPPPREISARAA
                     NRTDALPDSPEPAFSLVWPVAHLAYGTGVGAGFGLVRRMLPSSPVVTGLIVGGGVWAI
                     SYAGYLPALGLYPPPSDDSPPRPVTMIVAHAVYGIALARIYDRL"
     gene            complement(1464..>1734)
                     /locus_tag="VFQ54_07725"
     CDS             complement(1464..>1734)
                     /locus_tag="VFQ54_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164918.1"
                     /translation="YNQTKNNYEPYANSPDMVLPPLYFDPDGAQTIAELTPTISDYVD
                     QTFAQAVTGQMDIEQGWSDYLSTLSGMGLDNFISIQQDVYDKSKG"
BASE COUNT          323 a          526 c          571 g          314 t
ORIGIN      
        1 cctgcatcgc gggatgcgcc gccgcgacgt ggggcagcag actcgtgtgc tgcgcgatat
       61 tcggctcgga gcgcagcttc tcatccagcg gccattcctg tatatccttc tggatcgtga
      121 ggtgggctac ggttcgccag gtcaacgctg acttgatcgc ggtatcgatc gccgagcgag
      181 cgtgggcggg accggagacg cgttcgctgt aggcggcgac atccatgaag aggcgatcga
      241 gggggatgtc ctgctgataa ccggatccga tcatgtcgtg ataggtgtgt ccggtcagcg
      301 cgaggacggg tgcgccatcg cacttcgcgt cgtagaggcc gttcaggagg tggattccac
      361 caggtcccga cgtcgcgatg cagacgccga gctttccggt gtacttggca taggcgcagg
      421 ccgcgaacgc cgcggcttcc tcatggcgaa cctggatgaa gcgaatcttt tcctgccgcg
      481 tgcgcaatgc ctcgaagacg ccgttgatgc cgtcgccggg gagcccgaaa acgacatcga
      541 cgccccaatc gaggaggcgg tcgacgatga tgtccgatgt ggtcgtagcc atggaaaacg
      601 tccctttccg aactatcgga aactgaagtg caacactcct agtggtgcgt cactcctgag
      661 ttgccacgga gattccgtag cgggccaccc cgtgtggccc atgcggcgaa gccgcatctg
      721 atcgttggcg aagagcgccg gtaggcaaaa cactagaggc ggtcgtagat cctcgcgagc
      781 gcgatcccat agaccgcgtg ggcgacgatc atcgtcaccg ggcggggagg gctatcgtcg
      841 gagggtggtg ggtagagacc gagtgcgggg aggtagccgg cgtaactgat cgcccagaca
      901 ccaccgccga caatgagccc ggtcaccacc ggtgatgacg gcagcattct gcgaacgagc
      961 ccgaagccgg caccgacacc ggtgccatag gcgagatggg cgaccggcca gacgagggag
     1021 aaagcgggct ctggcgaatc cggcagcgca tccgtccggt tcgcggcgcg ggcgctgatc
     1081 tcgcggggcg gcgggttctg gaagaacccg agcttctccg cggcgacgat cggcacgctc
     1141 atcaacaggg tggcggcacc gccagccagt gccccgcgcg tcaatcgatg catggaatcc
     1201 tcgctttccc gttccccggg atcacgccga tcctggtcat gaagactcct ttcgcatatt
     1261 cggcaggtag caacggacct gatgcgatga ccgaatacgt gaggagtgcc catgcaccat
     1321 gtgtgccaga ccgtcgccgc cagcgccggc cgaccccggc gaaatccacg caagaacggg
     1381 agaaggggcc ccacgcgaca tcgcgtgagg ccccttctcc cggtttacct gatcgcatcc
     1441 ccagccggac gcgattgaat catctatccc ttgctcttgt cgtagacatc ctgctggatg
     1501 gagatgaagt tatccagccc cattccgctc agcgtgctga ggtagtccga ccatccctgc
     1561 tcgatatcca tctggccggt gaccgcctgg gcgaaggtct ggtcgacata atcggagatc
     1621 gtcggcgtca gctcggcgat cgtctgggcg ccgtccggat cgaagtagag cggcggcaac
     1681 accatgtccg gcgaattcgc gtatggctcg tagttgttct tcgtctggtt gtag
//
LOCUS       DASTMD010000529         4401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_943013, whole genome shotgun sequence.
ACCESSION   DASTMD010000529 DASTMD010000000
VERSION     DASTMD010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943013"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VFQ54_07730"
     CDS             complement(<1..753)
                     /locus_tag="VFQ54_07730"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEU0164919.1"
                     /translation="MATSTSTEAPAEDRATPSTTKVKATTTKKATTTKARKPKAARAP
                     RGKLVIVESPAKARSIGRYLGAGYTVKASMGHIRDLPKSTLGVDVDADFHPKYLIPRD
                     KSKVVKELKESVAGAKEIYLATDPDREGEAIAWHLLHATDAEDKPVHRVVFHEITPEA
                     VIAAIENPRDIDMDLVDAYQARRILDRLVGYGISPLLWKKVKRGLSAGRVQSAALRIV
                     AEREREIEQFIPVEYWSLDADLAKITGKKPTAR"
     gene            complement(1034..2155)
                     /gene="dprA"
                     /locus_tag="VFQ54_07735"
     CDS             complement(1034..2155)
                     /gene="dprA"
                     /locus_tag="VFQ54_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU0164920.1"
                     /translation="MGFDGVQPDVERERPFWVAFHRVPYVGPVSIRKLLERFGSLEAA
                     WHLDGRRLRAALNERAVASVESTRRELDVNQLMSDLERDRISVVTLAEEGYPRLLREI
                     HSSPPVLFYRGDLIPEDATAVAIVGTRQVTAYGRAVAEDLAGGLARAGITIVSGLAKG
                     VDGIAHRAALEAGGRTIAVLGGGINRLYPYEHRHLAERITTSGAVLSDYFPDSKPEGL
                     NFPARNRIISGLSLGVIVVEAPEKSGALITVDFAADQGRDVFAVPGPLGAERSAGCNR
                     ILRDGARLVRSADDVLEDLRLRGQAREVEVQQALPLGEDERRVLAVLTGEPMHIDDIC
                     EQANLSTPLVSAHLITMELQGLVRNAGAQHYVRSGRPGL"
     gene            2319..3086
                     /locus_tag="VFQ54_07740"
     CDS             2319..3086
                     /locus_tag="VFQ54_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0164921.1"
                     /translation="MQGNPIRDKMAAGEVVIGPFLSIASPLLVEIIALADFDFVILDG
                     EHGPLSADLIYPMILAAEARGISAFARVGELDKQVILKYLDVGVTGIMAPQIADAQQA
                     QTAIAATRFSPRGNRGLAGGRIFDFALRQPAPDLVQPLSDRVLTMIQFEHVDTLAHLD
                     EILDTPELDVLFVGPNDLAQSLGLPGQPGHPRVTEVADEVVARCKAKGIKTGTTISDV
                     ATAKATADRGFDMLVPNLPSLIAQASRSLVQGIRAAL"
     gene            3699..>4401
                     /locus_tag="VFQ54_07745"
     CDS             3699..>4401
                     /locus_tag="VFQ54_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0164922.1"
                     /translation="MAAEPVTTSANTGGIDRGSPLLEVKGLQTQFFTQDGVVKAVDNV
                     SFYVMPGETLGVVGESGSGKSITGLSIMRLIPSPPGKIVSGEVLFQGNDIRKMSDEQV
                     RAIRGNEIAMIFQDPMTSLNPVLTINRQISESLQLHMGMNKSQAKARSIELLQMVGIP
                     NAESRVDQYPHQFSGGMRQRVMIAMALSCNPKLLIADEPTTALDVTIQAQILDLMRTL
                     QNETGTGVIMITHSMG"
BASE COUNT          821 a         1421 c         1363 g          796 t
ORIGIN      
        1 ccgcgccgtc ggcttcttgc cggtgatctt cgcgagatcg gcatccagcg accagtactc
       61 gaccgggatg aactgctcga tctcccgctc gcgttccgcg acgatgcgca gcgcggcgct
      121 ctggacgcgg ccggccgaaa ggccgcgctt gaccttcttc caaagaagag ggctgatccc
      181 gtatccgacg agacgatcga ggatgcgccg cgcctggtag gcgtcgacga gatccatgtc
      241 gatgtcccgc ggattctcga tcgccgcgat caccgcttcg ggcgtgatct cgtggaagac
      301 gacgcggtga acgggcttgt cctcggcgtc cgtcgcatgg aggagatgcc aggcaatcgc
      361 ctcgccctcg cgatcggggt cggttgcgag atagatctcc ttcgcaccgg ccacgctctc
      421 cttcagttcc ttgaccacct tgctcttgtc gcgggggatc aggtacttgg gatggaagtc
      481 ggcatcgaca tcgacgccga gcgtgctctt gggaaggtca cggatgtggc ccatcgaggc
      541 cttgacggtg tagccggcgc caagatagcg accgatcgac cgcgccttgg ccggcgactc
      601 gacgatcacg agcttgcctc gcggggcacg cgccgccttc ggcttgcgcg ccttcgtcgt
      661 cgttgccttc ttggttgtgg ttgctttcac cttcgtcgtt gaaggagttg ctcgatcctc
      721 ggccggagcc tcggtcgaag tactggttgc catggagatt agatcctgtt cgttaacgag
      781 cttgcccggg tggcagtgac gtcctgccac ccatgtgtgc ggtcaagcct ggacccggtg
      841 ggacgagggc cggcaattat cgagcgtttt cgctcacccg tgccatggcc cctgagatgt
      901 ccccgggcca cactcttttc taaagtagga atagatgaag acggatgtca agagacgatg
      961 agaaatcaat tctgtgcaaa tgcttggaaa tggccatatc agcgcagacg aaacccgggg
     1021 gaaataccac tgctcagagc cccggtcgac cggatcgcac gtagtgctgc gcccccgcat
     1081 tccgcaccag cccctgcaat tccatcgtga tcagatgggc ggagacgagc ggcgtcgaga
     1141 gattcgcctg ttcgcagatg tcgtcgatgt gcatcggctc accggtcagg acagccagga
     1201 ctcgccgttc gtcctcacca agtggaagcg cctgctgcac ctcgacctcc cgggcctgtc
     1261 ccctgagacg caggtcctcg aggacatcat cggcgctgcg gacgagccgc gcgccatcgc
     1321 ggaggatgcg gttgcatccc gcgctccgtt cggcgccaag cgggccgggg acggcgaaga
     1381 cgtcacgccc ctgatccgcg gcgaagtcga cggtgatcaa cgcgccgctc ttttccggcg
     1441 cctcaacgac gatcacgccg agcgacagcc cggagatgat ccggtttcgc gccgggaagt
     1501 tgagcccttc gggcttgctg tcgggaaagt agtcggaaag cacggcgcca ctcgtcgtga
     1561 tccgttccgc aagatgacga tgttcgtagg gatagaggcg attgatgccg ccaccgagaa
     1621 cggcaatcgt ccgccctcct gcctcgagcg cggcccgatg cgcgatcccg tcgacgccct
     1681 tggcgagacc ggagacgatc gtgatcccgg cccgcgcgag tccgcccgca agatcctcgg
     1741 cgacggcgcg cccataggca gtcacctgcc gagtcccgac gatcgcgacc gctgtcgcgt
     1801 cctcgggaat cagatcgccg cgatagaaga gcacgggtgg cgacgagtgg atctcccgca
     1861 ggaggcgtgg atacccctcc tccgcgaggg tcaccacgga gattcgatcg cgttcgagat
     1921 cggacatcaa ttggtttacg tcaagttccc ggcgtgtgga ttcaactgaa gccacggccc
     1981 gctcgttgag cgccgcccgg aggcgacgac catcgagatg ccacgcggct tcgagactcc
     2041 cgaagcgttc cagcagcttg cggatactca ccgggccgac gtaggggaca cgatgaaacg
     2101 cgacccaaaa gggccgctcg cgctccacat ccggctgaac gccgtcgaat cccacgtgtg
     2161 ctcctcccgg ttcactccca gaacaccatt atccgagagc gagtccggtt tgcacaacac
     2221 gcgaatgtgc atagaatgcc gaccagcggc tactcgcgcg ttccgcatgc ccgatcaggg
     2281 cgtccggcgt ttgactgatc cctgggggaa ctcatttcat gcaaggcaat ccgattcgcg
     2341 acaagatggc ggcgggagag gtcgtgatcg gccccttcct ctccatcgcg tcacctctcc
     2401 tcgtcgagat catcgccctc gccgacttcg acttcgtcat cctcgatggc gagcacggtc
     2461 cgctctcggc ggatctgatc tacccgatga tcctcgccgc cgaggctcgc ggcatctctg
     2521 cgttcgcccg cgttggcgag ctcgacaagc aggtcatcct gaagtacctc gatgtcgggg
     2581 tgaccggaat catggcgccg cagatcgccg acgcccagca ggcgcagacg gcgatcgccg
     2641 cgacgcgctt ctccccccgt ggaaatcgcg gtctcgccgg tggccgcatc ttcgacttcg
     2701 cgctccgcca gccggcgccc gacctcgtgc agccgctgag cgaccgcgtc ctcaccatga
     2761 tccagttcga gcatgtcgac acgctcgccc acctcgacga gatcctcgac acgcctgaac
     2821 tcgacgtcct cttcgtcggc ccgaacgatc ttgcccagtc gctcggtctg ccgggccagc
     2881 ccggacaccc cagggtgacc gaagtcgccg acgaagtcgt ggcccgctgc aaggcgaagg
     2941 gcatcaagac cggaacgacg atcagcgatg tcgcgacggc gaaggcgacg gcggatcgcg
     3001 ggttcgatat gctcgtcccg aacctgccaa gcctgatcgc gcaggcctcc cgatcgctcg
     3061 tgcagggaat tcgcgccgct ctctagactc cgggcacaat ccatcgtggg agtcgtgcac
     3121 tctgcacacc atcgccccga tcttgctccc tcgaccggac gttcgttgca cggagtgacc
     3181 aaaacaactg gttaatgttt gtacgggatc gccggatgga cgcgtttcga gggcgaatct
     3241 gacacctttg accagtaaat cggccctccg ctgaagattt cgcggacatt tccgaacttg
     3301 tggtaatgca tcttgttcgc aaagcaggca tactatgcgg gagtcaacga tgaccatgtc
     3361 cacgcccacg attccggggt catcccgagc atcaaggcga ggcgatggtc gtcgctctgt
     3421 gttcgttcac gagccggtgg atggagcacg tgaccaatca cggtgcgtcg aggccgatcg
     3481 ccggaaccgc accaaaacac agggcaaacg gtacgcgcaa cgaggcgcgc cggatgatat
     3541 ggaacactga ccggttcctg cagtcgttcg tcacggtccg acggcgctgc gctcactgag
     3601 gcgggcctcg gatatgctcc gagcccaaca cgggaccggt tgtcgaatcg agccagttgt
     3661 ccaacgcagg aattgtcgtt tgggcgagag gagccaggat ggcggcagag ccagtaacca
     3721 catctgcgaa caccggcgga atcgatcgcg gttcgccact gctggaagtg aaggggctgc
     3781 agacgcagtt cttcacgcag gacggcgtcg tcaaggcggt cgacaacgtt tcgttctacg
     3841 tgatgccggg agagacactc ggcgtcgtgg gcgaatcggg atctggcaag agcatcacgg
     3901 gcctgtcgat catgcgactg atcccgagcc cgccaggcaa gatcgtcagc ggcgaagtgc
     3961 tcttccaggg gaacgacatc cgcaagatgt cggacgagca ggttcgcgcg atccgcggca
     4021 atgaaatcgc catgatcttc caggacccga tgacgtcgct caacccggtg ctcacgatca
     4081 accgtcagat cagcgagtcg ctgcagcttc acatgggcat gaacaagtcc caggcgaagg
     4141 cgcggtcgat cgaactgctc cagatggtcg gtatcccgaa cgcggaatcg cgcgtcgatc
     4201 agtacccgca ccagttctcg ggcggtatgc gccagcgcgt gatgatcgcg atggcgcttt
     4261 cgtgcaatcc gaagctgctc atcgcggacg agccgacgac ggcgctcgac gtgacgatcc
     4321 aggcccagat cctcgatctg atgcgcacgc tccagaacga gacgggcacg ggcgtcatca
     4381 tgatcaccca ctcgatgggt g
//
LOCUS       DASTMD010000530         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_49940, whole genome shotgun sequence.
ACCESSION   DASTMD010000530 DASTMD010000000
VERSION     DASTMD010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49940"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..523)
                     /locus_tag="VFQ54_07750"
     CDS             complement(95..523)
                     /locus_tag="VFQ54_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU0164923.1"
                     /translation="MFIRINAALAASRSKREDDNEKGFTLIELLVVVLIIGILAAIAI
                     PVYIGQQHSAQDAAAKSDLTNAKTAIIAYAAGNTSDTLPAVAAAADYNDYGWPKAPAG
                     TIGGTITSSTSFSLTECSASGKGFKVDQDTAPAEDAALTC"
     gene            complement(665..1897)
                     /locus_tag="VFQ54_07755"
     CDS             complement(665..1897)
                     /locus_tag="VFQ54_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU0164924.1"
                     /translation="MSNVTAYAYTARNSSGKVVKGKLEASSQSAVLAKLRTMGISPIT
                     IEESSAGTGLRMEIKVPGLSNRVKLKDLAIASRQMATMIEAGLSLIRALTILADQTEN
                     RELARVLGLVKADIEQGLSLSDAMAKHDKAFPPLMVHMIRAGETGGFLDQALTGIADN
                     FEADVKLRDEVKSAMTYPVIVLIIAVVAVVGMLLFVVPIFQKMFDGMGETLPVPTLIL
                     VVLSKNMAWILPVLVVLIIGFAVWWSRNKNTDVVRERWDKIRLKLPVFGKLTQKIAIA
                     RFTRTLATMLGAGVPILQALSIVGSTSGSWTIENAIKRVQESVRQGKSIAGPLADEPI
                     FPSMVVQMISVGEEAGSMEIMLEKVSEFYDSEVETMTKGLTSLIEPLMIVVLGSVIGY
                     MVIALYLPIFQASTTVPK"
     gene            complement(1900..>2216)
                     /locus_tag="VFQ54_07760"
     CDS             complement(1900..>2216)
                     /locus_tag="VFQ54_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164925.1"
                     /translation="LAATLQGVVSQTLVKKVGGGRAVATEVLVTTPAISNLIREGKTY
                     QIGSAMQAGRAQGMHTMDQHLADLVNAGQISHLSAEEKAHNQEDLKQLIQRAAADKAR
                     GY"
BASE COUNT          418 a          686 c          654 g          458 t
ORIGIN      
        1 atatcgcact cgctccgtct tcgcgatcac gagttcgacg ggacaggagt ttgtacaccc
       61 accccgccga actgcgaact agttcagtac ttcgctaaca agtgagggct gcatcctctg
      121 caggagccgt gtcctgatca actttaaatc ccttaccact agcactgcac tcagtcaggc
      181 tgaacgacgt actgctcgta atagtgcccc cgatcgtccc cgccggggcc ttaggccagc
      241 cgtaatcgtt gtagtcggca gcggcagcga cagccggaag tgtatccgac gtgttgcccg
      301 ccgcgtacgc gatgatcgcc gtcttcgcgt tggtgagatc ggactttgcg gccgcgtcct
      361 gcgccgagtg ctgctggccg atatacaccg ggatggcgat cgcagcgagg atgccgataa
      421 tgagaacgac gacgaggagc tcgatgagcg tgaagccctt ttcgttgtcg tcttcgcgct
      481 tggagcgcga tgcggcaagc gccgcattga tgcgaatgaa catgatgtcg attcctgtcg
      541 gtatgtgtat agggacaggt ggtcgtcccc ccaaaatctg gaactaaccc ccgatgacta
      601 tgttcttgcc tccagagcga gtgcgacagc cctcgaaggg gggcaattcg ggcaatccgt
      661 gcccctattt cgggacggtg gtggatgcct ggaagatcgg caggtagagc gcgatgacca
      721 tgtagccgat cacgctgccg agaaccacga tcatgagcgg ctcgatgagc gaggtcagcc
      781 ccttggtcat cgtctcgacc tccgagtcgt aaaattcgct gaccttctcg agcatgatct
      841 ccatgcttcc ggcttcttca ccgaccgaga tcatctgcac gaccatcgat gggaaaatcg
      901 gttcatcggc cagtggtccg gcgatgctct tgccctgacg aacagactcc tgcactcgct
      961 tgatcgcgtt ttcgatcgtc caactacctg acgtggaccc gacgatggag agcgcctgca
     1021 aaatcggcac gcccgcgccc agcatcgtcg ccagggtccg cgtgaaccgg gcgatggcga
     1081 tcttctgggt cagcttgccg aacaccggga gcttgagccg gatcttgtcc caacgctctc
     1141 tcacgacatc cgtgttctta ttccggctcc accacacagc gaacccgatg atgagcacca
     1201 cgagcaccgg cagtatccag gccatgttct tcgacagcac gacaaggatg agcgtgggca
     1261 ccggcagggt ctcacccata ccatcgaaca tcttctggaa gatcggtacg acgaacagca
     1321 gcataccgac gacggccacg accgcaatga tcagcacgat caccgggtac gtcatcgccg
     1381 acttgacctc gtcgcgaagt ttgacatcgg cttcgaagtt gtccgcgatg ccggtgagtg
     1441 cttgatcgag gaagccgccg gtctcacccg ccctgatcat gtgcaccatg aggggaggaa
     1501 atgccttgtc atgcttcgcc atcgcgtccg acagcgacaa cccttgttca atatccgcct
     1561 tgaccaggcc gagaacacgc gcgagctccc tgttctccgt ctggtcggcg agaatggtga
     1621 gagcgcgaat gagagagagc cctgcctcga tcatggtcgc catctggcgc gaggcgatcg
     1681 caagatcctt gagcttcacc cgattgctta gtcctggaac ctttatttcc atgcgcagac
     1741 cggttcccgc agacgactcc tcgatcgtga tcggcgagat gcccatcgtg cgcagcttcg
     1801 cgagcacggc gctctggctc gacgcctcga gcttgccctt gacgaccttg cccgacgaat
     1861 tgcgcgcggt gtaggcgtat gcggtgacgt tggacacgat cagtaccccc gagctttgtc
     1921 tgcggcagcg cgctggatga gctgcttcag gtcttcctgg ttgtgcgcct tctcctcggc
     1981 ggagaggtgc gagatctggc cggcgttgac gagatcggcg aggtgttgat ccatcgtgtg
     2041 catgccctgc gcgcgacctg cctgcatggc cgagccgatc tggtacgtct tgccctcgcg
     2101 gatgaggttc gagatggccg gcgttgtcac cagcacttcg gtcgcgaccg cgcggcctcc
     2161 gccgaccttc ttcacgaggg tctggctgac gacgccctgc agggtcgcgg cgagct
//
LOCUS       DASTMD010000531         4960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1300724, whole genome shotgun sequence.
ACCESSION   DASTMD010000531 DASTMD010000000
VERSION     DASTMD010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300724"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2514)
                     /gene="carB"
                     /locus_tag="VFQ54_07765"
     CDS             complement(<1..2514)
                     /gene="carB"
                     /locus_tag="VFQ54_07765"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HEU0164926.1"
                     /translation="MSARHKKPESVLVIGSGPIIIGQAAEFDYAGTQACRALREEGIR
                     TILINSNPATIMTDEDIADAVYIEPLTPDVIRRVIQRERPEGLLPTLGGQTGLNLAVQ
                     VAELGILEEYNVRLLGTPLEAIQKAEDRSLFKNLLQEIGEPAIPSAIVHTIAEAHAFA
                     ETVPLPLVIRPAYTLGGTGGGIAYTPEDLDRIVTSGLNASPIHQILLERSLLGWKEIE
                     YEVMRDSADTCITICNMENFDPMGVHTGDSIVVAPSQTLSDREYQMLRSSALNIIRSL
                     GIEGGCNVQYALDPNSMEYGVIEVNPRVSRSSALASKATGYPIARLSAKIAIGMRLDE
                     LTNVVTGKTTAAFEPALDYCVVKIPRWPFDKFPKADRTITTQMKATGEVMAIDRSFEG
                     AFQKAVRSLETTGKDVAWEDPAWGDDLEIIRSYVKRPTDLRLWAIMAALRRGVSVEEL
                     HELSSIDNWFLWKLEGIVAMEHELRGNPLDGPLLWEAKRAGFSDNQIGRLNGKPASEV
                     RDLRRSMGMNPVYKMVDTCAGEFAAQTPYFYSTYEEEDDATPIVGGRAVVVGSGPIRI
                     GQGIEFDYCSVQASRSLRENSVASIMINSNPETVSTDFDASDRLYFEPLDAESVEEVM
                     RHENGGLDDDNLPPMIVQFGGQTAINLAKPLTDVGAEILGSSLESIDLAEDRERFEAF
                     LRGLNIPQPTGSAVTSLHDAEAVANRIGYPVLVRPSYVLGGRAMEVVYGGDQLARYIR
                     NQVSLTPEHPVLVDKYLLGKEVEVDAICDGEQVLIPGIMEHIERAGVHSGDSFAVYPG
                     VNLFPAEVDTIVDYTTRIALNLNARGLINIQYVIHGGRIF"
     gene            complement(2605..3753)
                     /gene="carA"
                     /locus_tag="VFQ54_07770"
     CDS             complement(2605..3753)
                     /gene="carA"
                     /locus_tag="VFQ54_07770"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU0164927.1"
                     /translation="MSSHHHYAPPSNAALALEDGRVFRGMAFGAAADAAGEVVFTTTM
                     TGYQEVCSDPSFRGQIVCMTYPLIGNYGVNLDDDESRNPWIAGLVVRENCTAPSYWRS
                     MGTIDQYLKNAGIPGISGIDTRALTRHIREVGDTRAVIVHDVAHLSNEELVALAHKAP
                     LPGETDVVGEVQERGEVIYGNENTGPSVVVVDCGVKRNIIRSLLKRNARVTVVPYGTP
                     YSRIAEIDPDGVIVSPGPGDPANLDAGLDVVRALLADERPYFGICLGHQLLARAIGAE
                     TSKLKFGHRGGNHPVMDERNGRVTITAQNHSFRVDDETVPQGDGWQVALRNLNDNSVE
                     GLAHERLPVLSVQFHPEASPGPHDNGLLFDEFLQMIATPKSGAEALSL"
     gene            complement(3746..>4960)
                     /locus_tag="VFQ54_07775"
     CDS             complement(3746..>4960)
                     /locus_tag="VFQ54_07775"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU0164928.1"
                     /translation="LDHVHGVQEIDATGKIVSPAWIDIHVHLRQPGFDEKETVKTGTE
                     AAVAGGFASIACMPNTKPALDSPEVLRELAAVVEREGVARVYPIATITRGRTGGEAVD
                     FDALAEAGAIGFSDDGDTTANSLIMRHALEASVRLNRPVMVHCEDKALAAGAMNEGIV
                     STVLGIPGIPAIAEEIIIARDLMIAELTGGWLHVCHVSTGRGAELIAEAKKRGVRVTV
                     EVMPHHIVMSDDWVAGDRTLHNTEEEAGANGHPADPNTKVNPPLRPVADTEMLLKAIQ
                     DGVFDCFGTDHAPHAAPEKSGSYESAAFGLSGLEFAFPSTYALVRAGHISLNDLIRLW
                     TVEPAKILRSDLGTLSVGSPADITVFDPDLAWEVTPAALRTKSANTPLLGMTLRGRSI
                     LTLVNGEARHHV"
BASE COUNT          816 a         1619 c         1609 g          916 t
ORIGIN      
        1 gaagatgcgt ccaccgtgga tcacgtactg gatgttgatc aggccgcgtg cattgagatt
       61 gagcgcaatc cgcgtcgtgt agtcgacgat cgtgtcgacc tccgccggga agaggttcac
      121 gcccgggtag accgcgaacg aatcgcccga gtgcacgccg gcacgctcga tgtgctccat
      181 gatgccggga atcaggacct gctcgccgtc gcagatcgcg tcgacctcga cttccttgcc
      241 cagcaggtac ttgtcgacga gcaccggatg ttctggcgtc aggctcacct ggttgcggat
      301 gtagcgagcc agctgatcgc ctccatagac gacctccatc gcccgcccgc cgagcacgta
      361 tgaggggcgc acgagcaccg gatacccgat ccggttcgcg accgcctcgg catcgtgcag
      421 cgacgtcacc gccgagccag tcggctgcgg gatgttgagc ccacggagga aggcctcgaa
      481 gcgctcgcga tcttccgcga ggtcgatcga ttcgagcgag ctgccgagga tctcggcgcc
      541 gacatcggtc aacggcttcg cgagattgat ggccgtctgt cccccgaact ggacgatcat
      601 cggcggcagg ttgtcgtcgt cgagcccgcc gttctcgtgg cgcatcactt cctcgacact
      661 ctcggcgtcg agcggctcga agtaaaggcg atcggacgcg tcgaagtcgg tcgagaccgt
      721 ctccgggttg gagttgatca tgatcgaggc gacgctgttt tcccgaagtg agcgcgatgc
      781 ctgcaccgag cagtaatcga actcgatgcc ttgcccgatc cggatcgggc ccgacccgac
      841 cacgaccgcc cgcccgccga cgatcggggt cgcatcgtct tcttcctcat aagtcgagta
      901 gaagtacggc gtctgcgcgg cgaactcacc agcgcaagta tcgaccatct tgtagacggg
      961 attcatcccc atcgagcggc ggagatcgcg aacctcggac gccggcttgc cgttgaggcg
     1021 tccgatctgg ttgtcggaga agccggcgcg cttcgcctcc cagagcagcg gtccgtcgag
     1081 cggattgccg cgcagctcgt gctccatcgc gacgatgccc tcgagcttcc agaggaacca
     1141 gttgtcgatc gagctcagct cgtgcagctc ctcgacgctc acgccacgtc gcagcgccgc
     1201 catgatcgcc cagagccgga ggtcggttgg tcgcttcacg tacgagcgga tgatctcgag
     1261 atcgtcaccc caggcgggat cctcccaggc gacatccttg ccggtcgtct ccagcgagcg
     1321 caccgccttc tggaacgcgc cctcgaagga gcgatcgatc gccatcacct cgccggtcgc
     1381 cttcatctgg gtggtgatcg tgcgatcggc tttcgggaac ttgtcgaacg gccagcgtgg
     1441 aatcttgacc acgcagtagt cgagcgccgg ctcgaaggcg gccgtcgtct tgccggtaac
     1501 gacattcgtc agctcgtcga gtcgcatccc gatcgcgatc ttcgccgaga gccgggcgat
     1561 cggataaccg gtcgccttcg acgccagcgc cgaggagcgg ctgacgcgcg gattgacctc
     1621 gatcacgccg tattccatcg agttcggatc gagcgcgtac tggacgttgc aaccgccctc
     1681 gatcccgagc gagcggatga tgttgagcgc ggacgagcgc agcatctggt attcacgatc
     1741 ggagagcgtc tgcgaagggg cgacgacgat gctgtcgccc gtgtggacgc ccatcggatc
     1801 gaagttttcc atgttgcaga tcgtgataca ggtgtcggcg ctgtcgcgca tcacctcgta
     1861 ctcaatctcc ttccagccga gcagcgagcg ctcgagcagg atctgatgga tcggactcgc
     1921 gttgagcccc gaggtcacga tccgatcgag atcctccggc gtataggcga tgccgccgcc
     1981 ggtaccaccc agcgtgtagg cggggcggat caccagcggc agcggcaccg tctcggcgaa
     2041 ggcgtgcgcc tccgcgatcg tatggacgat cgcgctcggg atcgcgggct cgccgatctc
     2101 ctgcaggaga ttcttgaaga gcgagcggtc ttccgccttc tggatcgcct cgagcggcgt
     2161 tcccagcagg cggacgttgt attcctcgag gatgccgagc tccgcgacct gcacggcgag
     2221 attgagaccg gtctgcccgc cgagtgtcgg caggagaccc tccggtcgtt cgcgctggat
     2281 gacgcgtcga atgacgtccg gtgtcagcgg ctcgatgtag acggcatcgg cgatatcctc
     2341 atccgtcatg atcgtggccg ggttcgagtt gatgaggatg gtgcggatgc cttcctcacg
     2401 tagcgcacgg cacgcctgcg ttccggcgta gtcgaattct gccgcctgcc cgatgatgat
     2461 cggtccggaa ccgatcacca gcacgctctc cggcttctta tgtcgcgcgc tcaccaccgt
     2521 cattgtctcc ctcaccaacc catgctgcat cgtggcgtcc tggttgcccg gcgctcgtcg
     2581 tcgttgtctt cccggtctcg tcgtctacag gctcagcgcc tcggcgccgg atttcggtgt
     2641 cgcgatcatc tggaggaact cgtcgaagag gagcccgtta tcatgcggcc ccggcgacgc
     2701 ctccgggtgg aactgcaccg agaggacggg caggcgctcg tgcgccaggc cctcgaccga
     2761 gttgtcgttc agattgcgga gcgcgacctg ccagccgtca ccctgtggca cggtctcgtc
     2821 atcgacgcgg aagctgtgat tctgtgccgt gatcgtgacg cggccattcc gttcatccat
     2881 caccggatga ttgccaccgc ggtgaccgaa cttgagcttg ctcgtctcgg cgccgatcgc
     2941 ccgggcgaga agctggtgac cgaggcagat cccgaagtag ggccgttcgt cagcgagaag
     3001 cgcccgcacg acatcgaggc ccgcatcgag attggcggga tcaccaggac ccggcgagac
     3061 gatcacgccg tcgggatcga tctcggcgat ccggctgtac ggagttccat agggaaccac
     3121 cgtcaccctg gcgttacgct tcaggagcga gcggatgatg ttccgcttca cgccgcagtc
     3181 gacgacgacc acgctcgggc cggtgttctc gttcccgtag atcacctcgc cgcgttcctg
     3241 aacctcgccg acgacgtcgg tttcgcccgg cagcggcgcc ttgtgggcga gcgcgacgag
     3301 ttcctcgttc gagagatgcg cgacgtcatg gacgatcacc gcgcgcgtgt caccgacctc
     3361 acggatgtgg cgggtaaggg cgcgcgtgtc gatcccggag atacccggga ttcccgcgtt
     3421 cttgaggtac tggtcgatcg tccccatcga gcgccaatag gaaggcgccg tgcagttctc
     3481 gcgcacgacg agccccgcga tccacggatt gcgggattcg tcgtcgtcga gattgacgcc
     3541 gtagttgccg atcagcgggt aggtcataca gacgatctga ccgcggaacg agggatcgga
     3601 gcagacctcc tggtagccgg tcatcgtcgt cgtgaagacg acctcgccgg cagcgtcagc
     3661 cgcggcgccg aacgccatgc cacggaagac acgtccgtcc tcgagcgcga gcgcggcgtt
     3721 ggagggtggt gcgtagtggt ggtggctaga catggtggcg tgcttctccg ttgacgaggg
     3781 tcagaatcga gcgaccgcgc agggtcatcc ccagcagcgg cgtgttggcg cttttggtcc
     3841 tgagcgccgc gggagtcacc tcccaggcga gatcgggatc gaagacggtg atgtcggcgg
     3901 gcgatccgac actgagcgtc ccgagatcgc tccgcaggat cttggccggc tcgaccgtcc
     3961 agaggcggat cagatcgttg agcgagatgt gaccggcacg gaccagcgcg taggtcgatg
     4021 gaaacgcgaa ctcgagtcca ctcaggccga acgccgccga ttcgtaggat ccgctcttct
     4081 ccggcgcggc atgcggggcg tgatccgtcc cgaagcaatc gaagacaccg tcctggatcg
     4141 ccttcagcag catctcggtg tcggcgaccg gccgtagcgg tggattcacc ttcgtattcg
     4201 gatcggccgg atgtccattc gccccggcct cctcctccgt attgtggagg gtgcgatcgc
     4261 cggcgaccca gtcgtcgctc atcacgatgt ggtgcggcat cacctcgacc gtgaccctga
     4321 cgccacgctt cttcgcctcg gcgatcagtt ccgcaccccg gccggtactg acgtggcaga
     4381 catggagcca cccaccggtg agttcggcga tcatcaggtc gcgcgcgatg atgatctcct
     4441 cggcgatcgc cgggatgccc gggatgccga gcaccgtgga gacgatgccc tcgttcatcg
     4501 cgccggcggc aagcgccttg tcctcgcagt gcaccatcac cgggcggttg agacggacgg
     4561 acgcctccag ggcgtgacgc atgatcaggc tgttcgccgt cgtgtcaccg tcgtcggaga
     4621 aaccgatcgc gcccgcttcg gcgagggcat cgaaatcaac cgcctcgccg ccggtccgcc
     4681 ccctcgtgat cgtcgcgatc gggtagacgc gggcgacgcc ctcgcgctcc accaccgcgg
     4741 cgagctcgcg aagaacctcg gggctgtcga gcgccggctt cgtgttcggc atgcacgcga
     4801 tcgaggcgaa tcctcctgcc acggcggcct cggtgcccgt cttcacggtc tccttctcgt
     4861 cgaatcccgg ttgacgcagg tgaacgtgaa tgtcgatcca ggccggagag acgatcttcc
     4921 ccgtcgcgtc gatctcctgc acgccgtgga cgtggtccag
//
LOCUS       DASTMD010000532         3882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_407291, whole genome shotgun sequence.
ACCESSION   DASTMD010000532 DASTMD010000000
VERSION     DASTMD010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407291"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            475..1413
                     /locus_tag="VFQ54_07780"
     CDS             475..1413
                     /locus_tag="VFQ54_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU0164929.1"
                     /translation="MTSPEHFGRPVNRRGFVGAGAAAGTAAAIALGGMRLALTDVAAQ
                     ENAPGHTFVVAGLDGWPGENPTNTDVLMIARLDLAAMTVRALSIPRDLYVEIPDVGYD
                     RINRAFDHGATESDAAWHSGIEVTRQTILTNFGVRIDGAVTTTFDGFVGLVNALDGVD
                     LDNPYDLADDQYPDLDSGVKSIFYPAGETHLSGEQALEFARTRHQDGDDGRVMRQQLV
                     LLAMLEKVQTAADRGELADLTAQRASAVSDDLGDDLAALLMEAVSSLDPANVTFGTIA
                     PFLWSETLADSGLSVYQGDWGSLPCYVQAFLAGEAA"
     gene            1611..2960
                     /gene="tyrS"
                     /locus_tag="VFQ54_07785"
     CDS             1611..2960
                     /gene="tyrS"
                     /locus_tag="VFQ54_07785"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU0164930.1"
                     /translation="MTETLTASITGERPGNRLSAPEIARQNGVNPVDYLRQRGFVYDI
                     TDEDGLRAAFDNGTVTYYIGFDPTGPSLHAGHMVSIMLLASLQRMGHRPIAVGGGGTA
                     MVGDPTGKTSTREIITLETLDDNLKAITAQLGKYLDFEGDQFGDNPAAKLVNNADWLL
                     PLHYIDFLREIGRHFSVNEMLALETYRTRIEAGGLNFVEFNYRLVQSYDFLHLFREEG
                     CTLQMGGSDQWTNITGGVELIRKADQGKAFALVSPLITTASGEKMGKTGSGVRVWLDP
                     DQFSPYDYYQYWVNTEDSLVRTYLLQFTFLPNEEIAELTSVSGEALREAKRVLAFEAT
                     ALAHGVDAAAQAEAAARALFAGTGAAISADDATLPTTGIARGDLTAELTLADAFVRAE
                     LAKSRGEARRLASQGCLAIGEDRVDDVDVPFAEVLGSRDAVLLRAGKKRFKRVAIVG"
     gene            3532..>3882
                     /locus_tag="VFQ54_07790"
     CDS             3532..>3882
                     /locus_tag="VFQ54_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU0164931.1"
                     /translation="MGKVTAHMSISLDGFIAGPNDSVENPLGDDGIRLHDWMIGLMIW
                     RENHGLEGGITSRDDEIVEDVTRDNGAIVMGRRMFHNDGGPWTEPLWPGFWGDNPPFH
                     LPVFVVTHHPRPSLE"
BASE COUNT          686 a         1222 c         1267 g          707 t
ORIGIN      
        1 gatcggcaaa agtctcgtcg acgaggcgat cgcagccgcc gcaatcatcc tgatagacat
       61 ccacgagaat cggccggcgc aacttgcgcg ccagctgggt ggcttccttc caattgtgga
      121 gccaggtgat tccgggagtc ccggtgattg tggacggcgt agcgaccagg gtgttcatgg
      181 gttcctcacg tttgattagt gcctgttgca catcaccata atactccgcg ccggacgcaa
      241 ttcatatctg tccctgcctg ttccgatcgc atcaggcgag cggaacgcct ccgttggatg
      301 gctcgttgca ggggtgccga cgtggcaacg gatgcacggt cacacctgtc cgtccctggc
      361 gaagtggtat cgttcgcact tcgcagtcaa tgagcccctc gcgttcatgt tcgccacatt
      421 catcaccgtc gcgaggctgg cccgttttcg caccattgaa ggagcgtgat tgccatgaca
      481 tcacccgagc atttcggccg accggtcaat cgccgcggtt tcgtcggcgc cggcgccgct
      541 gctggcacgg ccgccgcgat cgccctcggc ggcatgcggc tcgccctcac cgatgtcgcc
      601 gcgcaggaga acgcgcccgg ccataccttc gtcgtcgcgg gtcttgacgg gtggccgggc
      661 gagaacccga cgaacaccga tgtcctgatg attgcccgtc tcgatctcgc cgcgatgact
      721 gtccgggccc tgtcgattcc gcgcgatctc tacgtggaga tcccggatgt tggttacgac
      781 cggatcaacc gcgcgttcga ccacggcgcg acggagagtg atgccgcctg gcactccggg
      841 atcgaggtga cgcggcagac gattctgacg aatttcgggg tacggatcga cggcgcggtg
      901 acgacgacat tcgacgggtt cgtcggcctc gtgaatgccc tcgatggagt cgatctcgat
      961 aacccgtacg atctcgccga cgatcagtat ccagacctcg attccggagt gaaatcgatc
     1021 ttctatccgg cgggcgagac gcatctctcc ggcgagcagg cgctcgagtt cgcacggacg
     1081 cggcatcagg atggcgacga tggccgggtg atgcgtcagc aactcgtcct gttggcgatg
     1141 ctggagaagg tccagaccgc ggccgatcgc ggcgagcttg ccgatctcac cgcgcaacgt
     1201 gcgtcggcgg tcagcgacga cctgggtgac gacctcgccg ctctcctgat ggaggcggtt
     1261 tcgtcgctcg atccggcgaa cgtgaccttc ggtacgatcg cgccgttcct gtggagtgag
     1321 acactcgcgg attcggggct gtcggtctat cagggtgact ggggtagcct gccttgttac
     1381 gtccaggcct tcctcgcagg ggaagccgcc tgattcgcgg caaggcgttg gccagcatcg
     1441 gcgagcatga atacgtccac gtcagttcac agggcgggcc gcgtgctcgc ccgtgatacg
     1501 tgtctgaaga gatgctcgcc ctgcacgata accgttcgcc gcggcgtaca attgacacgc
     1561 aagggtcgcg aatcgtcgcg gctgttctta tctcacgcca ggacggagtc atgaccgaga
     1621 cgctgaccgc atccatcacc ggggaacgac caggaaaccg cctcagcgcg cccgaaatcg
     1681 cgcgacagaa cggcgtgaat ccggtcgact atctgcggca gcgtggcttc gtctatgaca
     1741 tcacggacga ggacgggctc cgcgccgcct tcgacaacgg caccgtcacc tactacatcg
     1801 gcttcgaccc gaccggtccc tcgctccacg ccgggcacat ggtctcgatc atgctgctcg
     1861 cgtccctgca gcggatgggg caccgcccga tcgcagttgg cggcggtggc acggcgatgg
     1921 tcggcgatcc gaccgggaag acatccacgc gcgagatcat cacgctcgag acgctggacg
     1981 acaacctgaa ggcgatcacc gcccagctcg ggaagtacct cgacttcgag ggcgaccagt
     2041 tcggcgacaa tccggcggcg aagctcgtca acaacgccga ctggctgctg ccgctgcact
     2101 acatcgactt cctgcgcgag atcggtcgtc acttctcggt caacgagatg ctggcgctcg
     2161 aaacctatcg gacgcggatc gaggcgggtg ggctcaactt tgtcgagttc aactaccgcc
     2221 tggtgcagtc gtacgacttc ctgcacctct tccgggaaga gggctgcacc ctgcagatgg
     2281 gtggctccga ccagtggacg aacatcaccg gcggtgtcga gctcattcgc aaggcggacc
     2341 agggcaaggc cttcgcgctc gtcagcccgc tgatcacgac cgcgagcggc gagaagatgg
     2401 ggaagaccgg cagcggcgtc cgcgtctggc tcgatcccga tcagttctcg ccgtacgact
     2461 actaccagta ctgggtgaat acggaggaca gcctcgttcg cacctacctg ctgcagttca
     2521 ccttcctccc gaacgaggag atcgccgagt tgacctcggt cagcggcgag gcgttgcggg
     2581 aggcgaaacg cgtgctcgcc ttcgaggcga ccgcgctcgc gcatggggtc gatgcggcgg
     2641 cgcaggcgga ggcggccgca cgggcgctct tcgcgggcac cggcgccgcg atctccgccg
     2701 atgacgcgac gctgccgacg accgggattg cgcgcggcga tctcaccgcg gaactgacgc
     2761 tggccgacgc cttcgtccgg gccgagttgg cgaagtcccg cggcgaggcg cgccgcctcg
     2821 catcgcaggg ttgcctcgcg atcggtgagg atcgcgtcga cgacgtcgac gtcccgttcg
     2881 ccgaggtgct cggatcgcgg gacgcagtcc tgctccgggc cggcaagaag cgcttcaagc
     2941 gcgtcgcgat cgttggatag ccgagcgcgg catcggtgcg acaagtcgat agaaatacgc
     3001 aaagagcgcc acgttgttgg cgctctttgc gttgcgtgct gcctgaccca tcgggcagag
     3061 cccgatatga tcgttccatg aacgatgcct ggccggttgt ggtcgcttga tgaaccgtag
     3121 acgcgaccct tgtggtcgcc ccgggtcgcc gaaggcgatc cgtgttcgtg ggtgaatgac
     3181 gagaacagac gacgcgtcgc ccggatgctc ctgcttgagt ccggcggagt ttacgctgag
     3241 cttgccgaat gggcgggtgc gcctcgtgcg gggtttctgg acaggtgcga gatcgcagtc
     3301 cggggatcgg aggaatcgca aagcggacga tcatatcgag ctgcgctcga tgggccacac
     3361 ggggtggccc gctacggggc aaacgccgtc agcacgctcg tcctgagcga gaggtctatc
     3421 cccgggattc actgcccaga ctatgtcgag aaaccggggc caggtccgac tgttgagtga
     3481 gtggcatcca acgcgggatg tcgtgtcgtt ctgagccgaa tggagtcacc aatgggcaag
     3541 gtcaccgcac acatgtcgat ctctctggac ggattcatcg ccggaccgaa tgattcggtc
     3601 gagaacccac tcggcgatga cggcattcgg ctacacgact ggatgatcgg tctgatgata
     3661 tggcgcgaga atcacggact cgaaggcggc atcaccagtc gcgacgacga gatcgtcgag
     3721 gacgtcacca gggacaacgg tgcgatcgtg atgggacgcc ggatgttcca caacgacggc
     3781 ggaccgtgga cggaaccact ctggcccgga ttctggggcg acaatccacc cttccatctt
     3841 cccgtcttcg tcgtgaccca tcatccgcgt ccgtcgctgg ag
//
LOCUS       DASTMD010000533         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1658819, whole genome shotgun sequence.
ACCESSION   DASTMD010000533 DASTMD010000000
VERSION     DASTMD010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658819"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2296)
                     /locus_tag="VFQ54_07795"
     CDS             complement(<1..2296)
                     /locus_tag="VFQ54_07795"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEU0164932.1"
                     /translation="MTQATPTFPEHPRLFQDRTTRGAGRQDGLRESEPSGPFRQLQNA
                     LIASDPGLNRLIAAVQVVASMGAAIWAAWFFIHRTKVMWIEPPHGVTLPAAQLAALSA
                     QHHGITLLAMMMAGIIGLMASSAVMDPTPGGQILTMISMPIPMIATMAISIEMASHRT
                     PGIILMALVLGLGTYLRKFVPVIGPRAALYGMVLFNGYLTGFLSAGSLKTDQLGWIAV
                     ILWIGIFVSMVLKFIVFKPLDRGRFRRMTGSFLARSHDVISSAIALIDAKDERDRERA
                     HRRLRQRLTRLNETALVIDASLTTPGALPAGVTAYDAHEELFDIELMVQNVGSIVQQL
                     ADANLPGPLRAEIREWLVNLQERRSDLAVTSARAVTNGATTIAGVSENDMTRVRRLAA
                     AVEEWATVAAAWPSRSRELEERDRVEPFASRVNLILGNLPGSALVSRQATVTQPGFPG
                     HLAARLHLDAPAQIAIRVAVAVGVASWLGTLLNEQRFYWAVIAVFVIFLGTNTAAEQA
                     TKAVYRVIGTMIGIFAGSLLAHAIGLSVWSIAVILVAVGIGMYFAPINYAVMTFAVTI
                     SVSQLYEQLGEFSFHLLEIRVEETAIGAAIATLAALVIFPVNTRRAATIAAHGSVAAL
                     GTLLEQAVAKVRGHADGSLTGAARAVDAASQQLLTTARPLLLNPFRRDRLEYNLGLFG
                     HAAHQARNLAADIERHGRFDPATEPDVVHALEQERKSVLALQPVLDGQRDVAPPRRLD
                     LDYQSAHDRSRSRGDDAACRMLTEL"
BASE COUNT          510 a          917 c         1016 g          465 t
ORIGIN      
        1 ccagctcggt gagcatgcgg caggcggcat catcgccccg cgatcgtgac cggtcatgcg
       61 ccgactggta gtcgagatcg aggcgtcgtg gcggggccac gtctcgctgt ccatcgagca
      121 cgggctgaag cgccaggacg gacttccgct cctgctccag cgcatggacg acatcgggct
      181 cggtcgcggg atcgaaccgt ccgtggcgct cgatatcggc ggcgaggttg cgcgcctgat
      241 gtgccgcgtg gccgaagagc ccgagattgt actcgaggcg atcgcggcgg aaggggttca
      301 ggagcaacgg gcgcgccgtg gtgaggagtt gctggctggc ggcatcgacc gcgcgggcgg
      361 cgccggtgag gctgccatcg gcatgcccac gcaccttggc gaccgcctgc tcgagcagcg
      421 tccccagtgc cgcgacggat ccgtgcgccg cgatcgtggc cgcccgccgg gtgttgaccg
      481 ggaagatcac cagggcggcg agcgtggcga tcgcggcgcc gatcgccgtt tcctcgacac
      541 ggatctcgag cagatggaag ctgaactcgc cgagctgctc gtagagctgc gacacgctga
      601 tcgtgaccgc gaaggtcatc accgcatagt tgatcggcgc gaagtacatc ccgatgccca
      661 ccgcgaccag gatcaccgcg atcgaccaga cgctcagccc aatcgcatgc gcgagcagcg
      721 acccggcaaa gatgccgatc atcgtgccga tcacgcggta gaccgccttc gtcgcttgct
      781 cggcggcggt attcgtgccg aggaagatca cgaagacggc gatcaccgcc cagtagaatc
      841 gctgctcgtt gagcagcgtc ccgagccagc tcgccacgcc aacggcgacg gcgacgcgga
      901 tggcgatctg cgccggtgcg tcgaggtgca atcgcgcggc aagatgtccg gggaagccag
      961 gctgggtcac ggtggcctgg cggctgacga gggccgatcc cggcaggttg ccgagaatca
     1021 gattgacccg cgaggcgaag ggctcgacgc gatcgcgctc ttccagctcg cggctccgcg
     1081 acggccacgc ggcggcgacg gtcgcccatt cctcgacggc ggcggcgagc cggcgcacgc
     1141 gcgtcatgtc gttctcgctg acgccggcga tcgtcgtggc gccgttcgtg acggcgcggg
     1201 ccgaggtcac cgcgagatcg ctgcggcgtt cttgcagatt gacgagccac tcccggattt
     1261 cggcccgcag cggaccgggc agattcgcat ccgccagctg ctggacgatg ctcccgacgt
     1321 tctggaccat gagctcgata tcgaagagtt cctcgtgggc atcgtaggcc gtgacgccgg
     1381 cgggcagcgc acccggcgtc gtcagggagg catcgatcac gagcgccgtc tcgttgaggc
     1441 gggtcagccg ctggcggagc cggcgatggg ctcgctcacg atcccgctca tccttcgcgt
     1501 cgatcagcgc gatcgccgag ctgatgacgt cgtgggagcg cgcgaggaag gagccggtca
     1561 tccggcggaa acgaccgcgg tccagcggct tgaagacgat gaacttcagt accatgctga
     1621 cgaagatgcc gatccagagg atgacggcga tccagccgag ctgatccgtc ttgagcgatc
     1681 cggcgctcag gaaaccggtg agatagccat tgaagagcac catgccgtag agggcggcac
     1741 ggggaccgat gacggggacg aacttccgga ggtaggtgcc cagccccaac accagggcca
     1801 tcaggatgat gcccggtgtc cggtgcgacg ccatctcgat gctgatcgcc atcgtcgcga
     1861 tcatcgggat cggcatcgag atcatcgtca ggatctggcc gcccggggtc ggatccatga
     1921 cggcggagct cgccatcagg ccgatgatcc cggccatcat catcgcgagg agcgtgatgc
     1981 catgatgctg tgcggagagc gcggcgagtt gggcggccgg cagcgtgact ccatgcggcg
     2041 gctcgatcca catcaccttc gtcctgtgga tgaagaacca tgccgcccag atcgcggcgc
     2101 ccatgctggc gacgacctgg accgccgcga tcaggcggtt caatccggga tcggacgcga
     2161 tgagcgcatt ctggagttga cggaagggac cgctcggttc ggattcacga agcccatcct
     2221 gccggccagc gccgcgggtg gttcgatcct gaaagagccg ggggtgctcc ggaaatgtcg
     2281 gtgtggcctg ggtcatggga tggaagtctc cttcatagcc agggaccgcg cgaaatcggc
     2341 gcgatcgaag gcgacgtccg tccggggatc gcaaccagcg gcctcctggt caggagggtg
     2401 gtcgatcgag tggcggagaa cgggtgctcg gtggggattc atgaggatgc cgcgcacatg
     2461 cgcgcatcga tcgacgcggc cagccgggat gcatcgcatc tcgctcacca ttaccgccac
     2521 gtcagtcgcg cgccgttgcg caattcctca ttattgtatt gcgaatcgct gtgaaatggg
     2581 ggaatcagcg acgaatcata ttcggcattc cctcaccgca cttccgcatt cccgatcgac
     2641 gcggtgccgg aaacgaacgc ggaacgctcc cgccgctggc gtgcagggtg ggagcgttcc
     2701 tggagacctg accgggtgtg gaaagacgtg agccatcacg aaatcgaaac ggttgccaca
     2761 catgctgccg gatcggacgc aactttcaat acggcattcg gtcgttcgtg gggcgggacc
     2821 gccgctgccg gaccgtagtc cggacggcct gtcccgcctc ccgactagac cgctgcggcc
     2881 cactgctgca ggaggtcgca gtagaaga
//
LOCUS       DASTMD010000534         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_50256, whole genome shotgun sequence.
ACCESSION   DASTMD010000534 DASTMD010000000
VERSION     DASTMD010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50256"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFQ54_07800"
     CDS             <1..359
                     /locus_tag="VFQ54_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164933.1"
                     /translation="IGHAMLVRSMQQQAKRDEPDATIFLGTLLQAAPVALHRAAVSLL
                     AERTPTFREQLLGSDIPRTYIAGERSGALPQPDLIAAGVAYVQIANAGHVMMNDDPDA
                     FIAAIATATSDVAARN"
     gene            complement(443..1510)
                     /gene="recA"
                     /locus_tag="VFQ54_07805"
     CDS             complement(443..1510)
                     /gene="recA"
                     /locus_tag="VFQ54_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU0164934.1"
                     /translation="MSEREDRSRSVDMAIAQIERQFGKGSIMKMKPGEVNPMQIDAIP
                     TGSIALDLALGIGGVPRGRIVEIYGPESSGKTTLALHIVAEAQKMGGMAAIIDAEHAL
                     DPIYAGKLGVDLDTLLISQPDTGEQALEITETLVRSTALDVIVVDSVAALVPKAEIEG
                     DMGDSHVGLQARLMSQALRKLTGAISRSNTCVIFINQLRMKIGVMFGSPETTTGGNAL
                     KFYASVRMDIRRIETLKDGQDAIGIRARVKVIKNKVAPPFRQAEFDIMYNEGISVAGS
                     VLDVGTDLGIVRKSGAWFYLGEERLGQGRENAKIYLKSNPTVLEDIRSRIKAASPELT
                     SRLAFDGNEAVAVDEDGVIAG"
BASE COUNT          382 a          774 c          711 g          465 t
ORIGIN      
        1 cgatcggtca cgccatgctg gtgcgtagca tgcaacaaca agcgaaacgc gatgagcccg
       61 acgcgaccat ctttctcgga acgctcctcc aggcggcacc ggtcgcactt catcgcgccg
      121 ccgtttccct gctggccgag cgcaccccga cgttccgcga gcagcttctc ggcagcgata
      181 tcccgcgtac gtatatcgcc ggggagcgtt cgggggcgtt gccgcagccc gatctgatcg
      241 ccgcgggcgt ggcgtacgta cagatcgcga acgccggcca tgtgatgatg aatgacgatc
      301 ccgatgcatt cattgcggcg atcgccacgg ccacgagcga tgtcgctgcc aggaactgag
      361 cctgttccag cacagaaaga gacccgggac cgaagctggg ttcggtcccg ggtctcttcg
      421 cgtcaccttt tgcgcggcgg tatcagccgg cgatcacgcc atcttcatcg acagctacgg
      481 cctcgttgcc gtcgaaggca agccgggagg tcaactccgg cgaggctgcc ttgatccgcg
      541 atcgaatgtc ctcgaggaca gtcggattgc tcttgaggta gatcttggcg ttctcacgtc
      601 cctgaccgag ccgctcttcg ccaaggtaga accaggcgcc actcttgcgc acgatcccga
      661 ggtcggttcc gacgtcgagc acgctgccgg cgacagaaat accttcgttg tacatgatgt
      721 cgaattcggc ctggcggaac ggcggtgcga ccttgttctt gatcaccttc acgcgcgccc
      781 ggatgccgat cgcatcctga ccgtccttca gcgtctcgat ccgacggata tccatgcgga
      841 cggatgcgta gaacttcagc gcgttgccgc cggtggtcgt ctccggcgat ccgaacatga
      901 cgccgatctt catgcgcaac tggttgatga agatcacgca ggtgttggaa cgactgatcg
      961 caccggtcaa cttgcggagc gcctgactca tgaggcgggc ctggagaccg acgtgggaat
     1021 cgcccatgtc cccctcgatc tcggccttcg gcacgagcgc cgcgaccgag tcgaccacga
     1081 tgacatcgag cgcggtggaa cgcaccagcg tctccgtgat ctcgagcgcc tgctcgccgg
     1141 tatccggctg cgagatcaac aacgtatcga ggtcgacgcc gagcttgccg gcatagatgg
     1201 ggtcgagtgc gtgctcggcg tcgatgatcg cggccatgcc gcccatcttc tgggcttccg
     1261 ccacgatatg cagggcaagc gtcgtcttgc ccgaggattc aggtccgtag atctcgacga
     1321 tgcgacctcg gggtacgccg ccgattccga gtgccagatc gagggcaatg ctaccggtgg
     1381 ggatcgcatc gatctgcatc ggattgacct cgcccggctt catcttcatg atggagccct
     1441 tgccgaactg tcgttcgatc tgcgcgatgg ccatgtctac cgatcgtgat cggtcctctc
     1501 gctcgctcat gtatctttcc ctcgttctgg gactcgttga caagtccggc gtcttccggc
     1561 gctgatgcgc tcgaccctgg ggaacgctgt ttgtctcgtc ctgcgatgtt tcgacacatc
     1621 ttacgacgct gcgtccggag acatcctcac catttgaccg gagagtcacc atcctgacgc
     1681 ctacccgccc gtagcgacct tcgccacata cgaacaaacg ttctccttcg tgctaatcat
     1741 accctgtcgg gccccgtcaa atcaatgcct gcacgtgggt gtgaacggcg cgcggcgctc
     1801 gcccgaaagc gaacgtacga gccccgtttc gactgcccct gaccagcggt cacctgcggc
     1861 gagttccatc cccgtcgcgg tcggcgctcc cggtgcgcgg ttcgttctcg tcccggcgac
     1921 ccatgtggtc gcctcgggtc aatgcggcac agccgagccg tttccgcagc gcgtgtcgca
     1981 gggaatggcc gtggtcgggt ccggcggagt ttaccctgag cctgtcgaac gggccggatg
     2041 tgcttcgcac ggggatttct cgacaggctc gaaatgacaa acctctttgg gtttcatacc
     2101 tcgccgcaaa cacgaacgtc ccgtgcaaca cgccgtaccc ggggtggctc ccatcccgat
     2161 cgccgcccta gatgggcggc gtccccgcgt ccaccggaac cgtggtgggt tccggggccg
     2221 tcgtggcggt tggcacctca gtcgggatca ccgtcggttc ctcggtcggc tccagcgtcg
     2281 ccgtcggggc cggcacctcc gtcggtaccg ggatcggcgt caccggcggt cc
//
LOCUS       DASTMD010000535         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_675335, whole genome shotgun sequence.
ACCESSION   DASTMD010000535 DASTMD010000000
VERSION     DASTMD010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675335"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFQ54_07810"
     CDS             complement(<1..226)
                     /locus_tag="VFQ54_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164935.1"
                     /translation="MAHLFGRTLRQAPADAELISHQLMVRAGMIRPLGAGIYSLLPLG
                     YRVTRKIEQIIREEMDAIGGQELLMPVVHPA"
     gene            complement(308..859)
                     /locus_tag="VFQ54_07815"
     CDS             complement(308..859)
                     /locus_tag="VFQ54_07815"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU0164936.1"
                     /translation="MKIYTARGDNGQTDLLGERVGKDDPRIDLLGDLDETTSTIGLGR
                     AHANEEGTRIRLIQVQRDLYAMMAELAFTDATRPDAWRFPADRVAWLEAVIEAVTATV
                     TLPREFVLPGDTVPGAALDVARTVSRRAERLAVNLAQAGQISNPEILRYLNRLSSLLF
                     ILARAEDLHVTGEARLAKDLDRE"
     gene            1165..2328
                     /locus_tag="VFQ54_07820"
     CDS             1165..2328
                     /locus_tag="VFQ54_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0164937.1"
                     /translation="MVLSSSPRLGRTPQPTPAISHDGPIRVAVIGLGFGSAVHIPALK
                     YLDETDVIAVCARREERAKAIAARHSIPIASDDWREIVNHPDVDAVVIATPPYLHHQM
                     AIAALEAGKHILCEKPLARNLAETRDMVKLAQVANVVAMVNHEFRYLPVRQYVKELID
                     EGYVGDPHSASTIVYRSSLADPNERPYGWLMEHDKAGGMLGASGSHHIDTLRWWLGDI
                     NAVCGTTSTAVTHRRLPDSASMARVDADDNFAFILKFASGTIGTVHFSATAPTDAGEE
                     TTISGSEGMLIVQGDGQLYGARKRDLQLRELPIPERFKLKLPEFSHYLTRPTIMLIQE
                     WVRAMRTGAGASFSPSFSDGEKVQEIIDGVIRSSAQGRWIDTSTSRWPTAPTF"
     gene            2445..>2663
                     /locus_tag="VFQ54_07825"
     CDS             2445..>2663
                     /locus_tag="VFQ54_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164938.1"
                     /translation="MAATAPASELILSPDADIDTPALVVDEKILQHNIAEMAAFARSI
                     GVNLRPHIKTHKTPQIARLQIAAGAIGIT"
BASE COUNT          492 a          900 c          811 g          460 t
ORIGIN      
        1 ccgcgggatg cactaccggc atcagcagtt cctgaccgcc gatcgcgtcc atctcctccc
       61 ggatgatctg ttcgatcttg cgggtaacgc gataaccgag tggcaggaga ctgtagatcc
      121 cggcgcccag gggccggatc atccctgccc tgaccatgag ctggtgactg atgagttccg
      181 cgtccgccgg cgcctgacgc agcgtccgac cgaagagatg ggccatccgc atgattgaat
      241 ccgtcctccc gcacctgttc ctgtaattcg taggggaccc cgccaagctg gcaaaaccgc
      301 gcaaatccta ctcgcgatcg agatccttgg ccaatcgcgc ctcgcccgtg acgtgaaggt
      361 cctcggcccg cgccagaatg aagaggagcg aggagagccg gttgaggtag cggagaatct
      421 ccggattcga gatctgtccc gcctgcgcca ggttcaccgc cagccgttcg gcccggcgag
      481 agactgtccg cgcaacatcg agtgccgccc cggggacggt gtcaccggga aggacgaact
      541 cgcggggcag ggtcacggtc gcggtgacag cctcgatcac tgcttcgagc caggcgacgc
      601 gatccgcggg gaagcgccag gcatccggtc gcgtcgcatc ggtgaaggcg agttccgcca
      661 tcatcgcata gagatcgcgc tggacctgga tgagccgtat ccgcgtccct tcctcgttcg
      721 cgtgtgcacg tccgagtccg atcgtcgagg tcgtttcgtc gaggtcgcca agcaggtcga
      781 tccgggggtc atccttgccg acacgctcgc cgaggaggtc cgtctgccca ttgtcaccgc
      841 gtgccgtgta gatcttcatc gcttcatccc gatctcatct ctggaaagaa gaatcgcctc
      901 tggacaatgg tcgccgttcg cgctgccgat tgcacccttg agtcatcatg acgccgaaat
      961 aaggaatcgg atcgcggtgt cgggaacgta cgttcgctgt ggtaggataa gtggccaact
     1021 tgggtcgtga cgtcgtcttt tcatcaactc gcaattcccg tgtgacacgt cgccaaccac
     1081 gccagttccc gatcccgacc gcacgcggta aatcgactca cctgccagca acagctcatg
     1141 cttcaccggg agaaacgtct acccatggtt ctgtcgtcct ccccacgact gggacgcacc
     1201 ccacaaccca cgcccgcgat atcgcacgac ggcccgatcc gggtcgccgt gatcggtctc
     1261 ggcttcggca gcgccgttca cattcccgcg ctcaaatatc tcgacgagac ggatgtgatt
     1321 gccgtctgcg cccgccgcga ggaacgcgcg aaggcaatcg cggcgcgcca ctcgatcccg
     1381 atcgcctcgg acgactggcg cgagatcgtc aaccatcccg atgtcgacgc ggtcgtcatc
     1441 gcgacaccgc cttatctgca tcaccagatg gcgatcgccg ccctggaggc cggcaagcac
     1501 atcctttgcg agaagccgct cgcccgcaat ctcgccgaga cgcgcgacat ggtgaagctc
     1561 gcccaggtcg cgaacgtcgt ggcgatggtc aaccacgagt tccgctatct gccggtccgg
     1621 cagtatgtga aggagttgat cgacgagggg tatgtcggag atccccactc cgcgtcgacg
     1681 atcgtctatc gctcgagtct cgcagatccg aacgagcgac cgtacggctg gctgatggag
     1741 cacgacaagg cgggtgggat gctcggcgca tcgggatcgc accacatcga tacgctccgc
     1801 tggtggctgg gagacatcaa cgccgtctgc gggacgacgt cgacggccgt cacccaccgg
     1861 cgacttcccg attccgccag catggcgcgg gtcgatgccg acgacaactt cgccttcatc
     1921 ctgaagttcg cgagcggcac gatcgggacc gtccacttct ccgcgacggc gccgaccgat
     1981 gccggcgagg agacgacgat ctccggttcc gagggcatgc tgatcgtcca gggcgatggc
     2041 cagctgtacg gcgcacgcaa gcgcgacctc cagctgcgcg agcttccgat cccggagcgc
     2101 ttcaagttga agctccccga gttcagtcac tacctgacgc gaccgacgat catgctgatc
     2161 caggagtggg tcagggcgat gcgaacggga gcgggcgctt cgttctcgcc gtccttcagc
     2221 gacggcgaga aggtccagga gatcatcgac ggcgtcatcc gctcgagtgc gcagggccgc
     2281 tggatcgata ccagcacgtc ccgctggccc accgcgccga cattctagcg aaggtcgttc
     2341 cgggggtcgg gaggccggtt ttgcgggaag ccttcccagg tgcaagaatg acgcaacgcg
     2401 cgaccggcgc cgacgctgca ttcactccgg gaggacctcc gctcatggcc gccacggcac
     2461 ccgcatccga gctcattctg tcgccggatg ccgacatcga cacccccgca ctcgttgtcg
     2521 acgagaagat cctccagcac aacatcgcgg agatggccgc cttcgcgagg agcatcggcg
     2581 tcaacctgcg tccccacatc aagacgcaca agacaccaca gatcgcccgc ctccagatcg
     2641 ccgcgggcgc gatcggcatc acc
//
LOCUS       DASTMD010000536         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_764259, whole genome shotgun sequence.
ACCESSION   DASTMD010000536 DASTMD010000000
VERSION     DASTMD010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764259"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFQ54_07830"
     CDS             complement(<1..244)
                     /locus_tag="VFQ54_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164939.1"
                     /translation="MSYDVSAVTTYLTDHYPELIAEASITDLDPVVSDVTAIYIAYPT
                     LDTGWANPLADYYFLKRVAAAFAPNFDLSDQTGALSL"
     gene            complement(241..564)
                     /locus_tag="VFQ54_07835"
     CDS             complement(241..564)
                     /locus_tag="VFQ54_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164940.1"
                     /translation="MASQSGMNRRQKNAEIRIQAVLESMAARSEVELTELPSRRNALV
                     QDTQRIEWIADALEQISAKVWPEPAPKGRKARATSAETASAEGNEPSAEPEAAPFVQT
                     MEPEA"
     gene            complement(582..1655)
                     /locus_tag="VFQ54_07840"
     CDS             complement(582..1655)
                     /locus_tag="VFQ54_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164941.1"
                     /translation="MGQLAGILHAEDSTLLPQAMTAPYADLTTAINTVLAAYTKARDE
                     AFALLVQETTPLRSERIRTAGIIRGQRIDADGRPLEERIGDFYEVAYPWERLGYAMGW
                     GSEDIQYLTIGDVQMNVDAIVAGNRADNIQAIWTRIYDNTAWSHTDLYGSLSIKPLAN
                     GDATPYAQPNGSAVTANNYLVSGYAEGSISATNNPLRQVRDKVWTFFPRTRVVALINP
                     LQLPTLQNNLPNFVDASVVGVTAGSGESVATPLDGAPQIPGDFAGIDRDTGVYVYTYD
                     TYVPDGYIDGIAIGAPAPLKRRWPEPPALRGFKLEAEQDHDPFHKQIYRERFGYGAAN
                     RLSMAVLQLKASGTYDIPTGFAR"
     gene            complement(1674..2120)
                     /locus_tag="VFQ54_07845"
     CDS             complement(1674..2120)
                     /locus_tag="VFQ54_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164942.1"
                     /translation="MADITKTAALCREIEVIENRHLALVTDEDIAAGDVIYVKSNGRA
                     AKARANAVGTAKIVGIATRAAAAGTPVTALYHGRYAGWYLSGVDSNKTVYLSTATAGA
                     IADAAATGTGNVVAAVGRVFAYTNPTAQRYVFVDISLNASDPVALS"
     gene            complement(2133..2810)
                     /locus_tag="VFQ54_07850"
     CDS             complement(2133..2810)
                     /locus_tag="VFQ54_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164943.1"
                     /translation="MFIRRLFAPIAFSLPMLSPEDSPAGETKVTEPVTETVAKVDPPK
                     VEEPTKVADPAKVDKPDKVEFNEAQQQKFNDAIAAVRKTTEETVRAAIAAEAAAAKQK
                     ADEDTAREEAEKRGEFDKVKTDLEGKLATAEDALKTTTDELTNLRELFTEHVKTQTKE
                     WSKELKDLLASADAPVKDQVAQMTKVQKLADEMKKTTGRGNGPDPDPAGKGKVDVTSP
                     ISKANMW"
BASE COUNT          569 a         1081 c         1068 g          715 t
ORIGIN      
        1 tcagggagag cgctccggtc tgatcgctca gatcgaagtt cggggcgaac gctgcggcga
       61 cccgcttcag gaagtagtag tccgcgagcg gattcgccca tcccgtgtcg agcgtcgggt
      121 aggcgatata gatcgccgtc acgtccgaca cgaccggatc gaggtccgta atcgacgcct
      181 ctgcgatcaa ctccggatag tgatccgtga gataggttgt gaccgctgag acgtcgtaac
      241 tcatgcctca ggctccattg tttgaacgaa tggggccgct tccggctcag cggagggctc
      301 gttcccctcc gctgacgcgg tctcagccga tgtcgcccgt gccttcctgc ccttaggcgc
      361 aggttcgggc cacaccttcg cgctgatctg ttcgagcgcg tcggcgatcc actcgatccg
      421 ctgcgtgtct tgcacgaggg cgttgcggcg cgaggggagc tcggtcagct ccacctcgct
      481 cctggccgcc atcgattcca ggaccgcctg aatgcggatc tccgcattct tctgacgtcg
      541 attcatccct gattgactag ccatcgcaat cgacctcccc gttaccgcgc aaagccggtc
      601 gggatgtcat aggtcccgct cgccttgagc tggagcacgg ccatgctgag gcgattcgcg
      661 gcgccgtaac cgaagcgctc gcgatagatc tgcttgtgga agggatcatg atcctgttca
      721 gcctcaagct tgaagccgcg cagggccggg ggctccggcc accggcgctt cagtggagcg
      781 ggagcgccga tcgcgatacc gtcgatatac ccgtccggga cgtaggtgtc gtacgtgtag
      841 acgtagacgc ccgtgtcgcg atcaatgccg gcgaagtcgc ccgggatctg cggcgctcca
      901 tccagcggcg tggcgactga ttcacccgag cctgcggtga caccgacgac cgatgcgtcg
      961 acgaagttcg gcaagttatt ctgcagggtt gggagctgca gcgggttgat caacgccacg
     1021 acgcgcgtcc gcgggaagaa ggtccagacc ttgtcccgca cctggcgtag cggattgttc
     1081 gtcgcgctga tgctgccctc ggcgtatccc gagacgagat agttgttcgc cgtcacggcg
     1141 ctgccattcg gctgcgcata gggtgtcgcg tcaccgttgg cgagcggttt gatcgagagt
     1201 gagccgtaaa gatctgtgtg cgaccacgcg gtgttgtcgt agatgcgtgt ccagatcgcc
     1261 tggatgttgt cggcacgatt accggcaacg atggcgtcga cgttcatctg tacgtcgcca
     1321 atggtgaggt attggatgtc ctcggaaccc catcccatcg cgtagccgag gcgttcccac
     1381 ggataggcaa cctcgtagaa gtcaccgatc cgctcttcga gcggtcgtcc gtcagcgtcg
     1441 atccgctgac ctcggatgat gccggcggtg cggatgcgct ctgaacgaag cggtgtggtc
     1501 tcctggacga gcagggcaaa cgcctcgtcg cgcgccttcg tataggctgc cagcaccgtg
     1561 ttgatcgccg tcgtcaggtc ggcgtagggg gccgtcatgg cctgtggcag cagggtcgag
     1621 tcctcggcat ggagaatacc ggcgagctgt cccatagcag aaacctcctt ggcttaggac
     1681 aacgcaaccg gatcggatgc gttcagcgaa atgtcgacaa agacgtagcg ttgggctgtg
     1741 gggttggtat acgcgaacac gcgacccacg gccgctacga cattccccgt tccggtcgcg
     1801 gcggcatccg caatcgcgcc ggcagtggcg gtggagagat agaccgtctt gttcgaatcg
     1861 acgccggaaa gataccagcc ggcatatcga ccgtgataca gcgcggtcac aggcgtcccg
     1921 gccgcagcgg cacgggtcgc aatgccaacg atcttcgctg tcccgaccgc gttggcacga
     1981 gccttcgctg cgcgaccgtt cgacttgacg tagatgacgt cgcctgccgc gatatcctcg
     2041 tcggtgacga gcgcgaggtg ccggttctcg atgacttcga tttcccgaca aagtgcggcc
     2101 gtcttggtga tatcagccat gcttggcctc cgttaccaca tattggcctt ggagatgggc
     2161 gacgtcacgt cgaccttgcc ctttccggcc ggatcgggat cgggaccgtt gccccgacca
     2221 gtcgttttct tcatctcatc cgcgagtttc tggaccttcg tcatctgtgc gacctggtcc
     2281 ttgaccgggg catcggcaga cgcaagcaaa tccttcagtt ccttcgacca ctccttggtc
     2341 tgggtcttca cgtgttcggt gaataactcg cggagattgg tgagttcatc ggtcgttgtc
     2401 tttagtgcgt cctcggcggt cgcgagcttg ccttcgagat cggtcttcac cttgtcgaac
     2461 tcgccccgct tctccgcctc ctcccgtgcc gtgtcctcat ccgccttctg ctttgccgct
     2521 gcagcctcag cggcgattgc cgcgcgaacc gtttcctcgg tcgtcttccg caccgccgca
     2581 atcgcatcgt tgaacttctg ctgctgcgcc tcgttgaact cgaccttgtc gggcttatcg
     2641 actttcgccg gatcggcgac ctttgtcggc tcttccacct tcggcggatc gaccttggcg
     2701 acagtctccg tgaccggttc ggtcaccttg gtttcacccg ctggcgaatc ctctggcgag
     2761 agcatcggga gcgagaatgc aatcggggcg aacaagcgtc gaatgaacat gcgatgtgcc
     2821 tccccgagca atctccggcc cggtacggaa tcccccggca gaacggccgg tgcgtggcgt
     2881 cgtctcaccc cggaacttga cggcatccgg tcgccggaca aacaaaaaac ccgctctcga
     2941 tgagagcggg cgatgcgctg gcgcggtggc gggcccttcc gaaagcgggc gatgccgacg
     3001 cgatggtcgg gtggccctgt gttcgattac gcacattatg acatatcggc tggccggaac
     3061 aaggatatcc gctcgaggtg ctccggagca tcccgagcgg gatgaactga ctatttccct
     3121 aacgtccggc ctgtgcccca cgcgaacggc caggcttcgg gcccctttct ctcctacgcg
     3181 ggactgaaca ttgcccttgt ggtgagggcc tcatccatga ttggcgcttg ggcgggagtg
     3241 gggattggct cgggggcgtg gcaaatcaat accggtatgt cgatcctccg ggaaatcgcc
     3301 tcagcgacgc ttcccaacgc caatcggctg aggccactcc gcccatgcga tgcaatggcg
     3361 accaggtctc cggcgcccgc cgcattccgg atctcgtcag ccgcccgacc ccgacgcaat
     3421 tcgaccgtga tcg
//
LOCUS       DASTMD010000537         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_318443, whole genome shotgun sequence.
ACCESSION   DASTMD010000537 DASTMD010000000
VERSION     DASTMD010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318443"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /gene="ilvN"
                     /locus_tag="VFQ54_07855"
     CDS             <1..483
                     /gene="ilvN"
                     /locus_tag="VFQ54_07855"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU0164944.1"
                     /translation="VVSLLRRRNFNIDSIAVGHSEQPGFSRMTLMVTGEDNEVEQAGK
                     QLYKLLEVIKVTDLLPGQAITREMVLVKVAAKGGQRAEIMQIAEVYGARILDASPSSL
                     MIEATGSEDQIESFLNMMRIFGIREMVRGGKIALSRGAGAKSQGRIDRINDDNFAVAD
                     "
     gene            611..1651
                     /gene="ilvC"
                     /locus_tag="VFQ54_07860"
     CDS             611..1651
                     /gene="ilvC"
                     /locus_tag="VFQ54_07860"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU0164945.1"
                     /translation="MPAVIYYENDATLAPIQDKTIAIIGYGAQGHAHALNLRDSGLSV
                     IVGLHEGSRSREKAAAQGLRVENVADAAAAADVIMVLVPDHLQKAIYTESIAPHLTAG
                     KMLMFAHGFSIHFGQIEPPTDVDVTMIAPKSPGSRVRELFEEGIGVPALVAVHQDATG
                     HAKEKALAYARGIGALSAGVLETTFKEETETDLFGEQAVLCGGVSALITAGFETLVEA
                     GYEPESAYFECLHEMKLIVDLIYEGGLTLMREVISDTAEYGDYYAGKQVITGDVKTAM
                     KQLLTRIQDGDFAREWIAENEAGRPNFLNLRQQANDHEIERVGAQLRGMMPFLKSGKA
                     ARAAAQAAQTAQ"
     gene            1751..2458
                     /locus_tag="VFQ54_07865"
                     /pseudo
     CDS             1751..2458
                     /locus_tag="VFQ54_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
BASE COUNT          484 a          752 c          795 g          427 t
ORIGIN      
        1 gtcgtctcct tgctccggcg ccgaaacttc aacatcgatt cgatcgccgt cggccacagc
       61 gaacagccgg gtttctcccg gatgacgctg atggtgaccg gcgaggacaa cgaggtcgag
      121 caggcaggca agcaactcta caaactgctc gaagtgatca aggtgacgga tctgttgccc
      181 ggtcaggcga tcacgcgtga gatggtcctg gtgaaagtgg ccgccaaggg cgggcagcgc
      241 gccgagatca tgcagatcgc ggaagtctat ggtgcgcgca tcctggatgc gtcgccgagt
      301 tcgctgatga tcgaggcgac ggggtccgag gaccagatcg agtcgttcct gaacatgatg
      361 cgcattttcg gaattcgtga gatggttcgc ggtggaaaga tcgcgctgtc acgaggagcc
      421 ggggcaaaat cacaggggcg gatcgaccgt atcaacgatg acaatttcgc agtcgcagac
      481 tgattcgttt cgagggagca tttgctccct tctcttttga cggtagcctg gcgcccagtt
      541 ccaggatcac gactcgcgag gatcgcgagc gaaacaggcg agcgacgatg gtcgcttcag
      601 gaggttttca atgccggcgg ttatctatta cgagaatgat gcgacgcttg ccccgatcca
      661 ggacaagacc atcgcgatca tcggctatgg cgcccagggt catgcgcatg ccctcaacct
      721 gcgcgattcc ggactcagcg tgatcgtcgg cctccatgaa ggcagccgca gccgcgagaa
      781 ggccgccgcc caggggctgc gcgtcgagaa cgtcgccgat gccgccgccg cggccgatgt
      841 gatcatggtc ctcgtgccgg atcacctcca gaaggcgatc tacacggagt cgatcgcgcc
      901 gcacctgacg gccggcaaga tgctgatgtt cgcgcacggc ttctcgatcc acttcggtca
      961 gatcgagccg ccgaccgatg tcgatgtcac gatgatcgcg ccgaagtccc cggggtcgcg
     1021 cgttcgcgag ctcttcgaag agggaattgg ggttcccgcc ctcgttgccg tccaccagga
     1081 cgccacaggt catgcaaagg agaaggcgct cgcgtatgcg aggggcatcg gtgccctctc
     1141 cgcgggcgtg cttgagacga ccttcaagga agagaccgag acggatctct tcggcgagca
     1201 ggccgttctc tgcggcggcg tctcggcatt gatcaccgcc ggcttcgaga cgctggtcga
     1261 ggctggctat gagccggaga gcgcctactt cgagtgcctc cacgagatga agctgatcgt
     1321 cgacctcatc tatgaaggtg gtctcaccct catgcgcgag gtcatctccg acacggccga
     1381 gtacggcgac tactacgccg gcaagcaggt gatcaccggc gacgtcaaga cggcgatgaa
     1441 gcagctgctg acccgcatcc aggatggaga cttcgcccgc gagtggatcg ccgagaacga
     1501 ggccggtcgc ccgaacttcc tgaatctccg ccagcaggcc aacgatcacg agatcgagcg
     1561 cgtcggcgcc cagcttcgtg gcatgatgcc gttcctgaag tcgggcaagg cggcacgtgc
     1621 cgctgcccag gcggcgcaaa ccgcccagta gagcaccggt aaccacgaaa gaagaatcac
     1681 catgaccgct tcggagagcc acgtagatag ccgagtcatc gttttcgata ccacctagcg
     1741 tgatggcgag cagtcgccgg gggcaaccct tacggctcag gagaagctcg agattgccga
     1801 cgccctcgtg gcattgaacg ttgatgtgat cgaggcgggt tttcccgccg catctcccgg
     1861 ggacttcgag gcggtcaagg cgatcgcgac gcgggcgaag ggcgtgaccg tcgccggtct
     1921 tgcccggacc gtgccgggcg acatcgaacg tgtcgcccag gccgtgaagc cggccgacca
     1981 gccgcgcatc catacgttca tcgcgacgtc cgacatccat ttgaagcaca agctgcggat
     2041 gagccgcgag caggtcctgg cgcgcgtcca ggagagcgtg acgttcgcga agacattcct
     2101 gagcgacgtc gagttctcct gcgaggacgc gacccgttcc gatcccgatt tcctggtcca
     2161 ggtcttccag accgcgatcg atgccggggc gacgacgatc aacgttccgg ataccgtcgg
     2221 ctacacgaca ccgggcgagt acgtcagcct gatcgagtac ctgcgggcga atgtccgcga
     2281 tatcgacaag gcgatcatct ccgttcactg ccacgacgat ctcggaatgg cgacggcgaa
     2341 cacgctggcg gcggtcgccg ctggtgcgcg tcaggtcgaa gtgacgatga acggaattgg
     2401 cgagcgagcg ggcaacacct ccctcgagga agtcgtgatg gccctccaga ctcgcggc
//
LOCUS       DASTMD010000538         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_50800, whole genome shotgun sequence.
ACCESSION   DASTMD010000538 DASTMD010000000
VERSION     DASTMD010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50800"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /gene="kdpC"
                     /locus_tag="VFQ54_07870"
     CDS             <1..558
                     /gene="kdpC"
                     /locus_tag="VFQ54_07870"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HEU0164946.1"
                     /translation="AITGIAYPLVMTGIGQVAFADKANGSLIKDGDTVVGSSLLGQSF
                     VDPKTDLTLSGYFRSRPSAAGSGYDASLSSGSNLGPTNQVLLDRIATDSAAIRKENGL
                     PADAELPVDLVTASGSGLDPDISPAAAEIQVARVAKQRGISEDQVEKLVKDNTSGRTL
                     WIFGEPRVNVLKLNLALDALTKPGS"
     gene            1095..>2143
                     /locus_tag="VFQ54_07875"
     CDS             1095..>2143
                     /locus_tag="VFQ54_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164947.1"
                     /translation="MPIAGDDGTYPHVTVGESGSGQAPARGRRGSLRILLGAAPGVGK
                     TYAMLRDAHRLRDEGNDVVIGFIETHGRQETENQIGDLEVVPRAKIIYQGVTLEEMDT
                     DAIVRRHPKYVLVDELAHTNAPGSPREKRYEDVAYLRDRGINVIGALNIQHLASMQDL
                     VAGITGIEVRESVPDHILTDATEVQLVDLPVEALIERIEQGKVYPPERSTRALQNFFQ
                     PGNLNALREMALRRTAEGVDERLADMMLGRAASATGDLVTPNERVAVVVEPDAEWGDV
                     LRNAWRLASSMKAELIALTIAPLGLLSEFSSERANKLRRQQELAEDLGARVDVVSSDG
                     GGMEDDVAAIVRALKA"
BASE COUNT          369 a          652 c          760 g          362 t
ORIGIN      
        1 gccatcacgg gtatcgccta cccgctggtg atgacgggga tcggccaggt ggcctttgcc
       61 gacaaggcga atggttcgct gatcaaggac ggcgacaccg tcgtcggttc ctcgctgctc
      121 ggccagagct tcgtcgaccc gaagacggac ctcaccctga gcgggtactt ccgcagccgg
      181 ccgtcggccg ccggttccgg ctacgacgcc agtctctcgt ccggttccaa tctcggcccg
      241 accaaccagg tattgctcga tcgcatcgcg accgactcgg cggcgattcg caaggagaat
      301 ggcctccccg ccgatgccga gctcccggtc gacctcgtga cggcctcggg cagtggtctc
      361 gatccggaca tcagcccggc ggcggccgag atccaggtgg cgcgtgtcgc gaagcagcgc
      421 gggatctcgg aagaccaggt cgagaagctg gtcaaggaca acacgtcggg ccggacgctc
      481 tggatcttcg gcgagccgcg cgtgaacgtc ctcaagctga acctggcgct cgacgccctg
      541 acgaagccgg gttcgtaggg gcgatcctgg tgatcgccct ggtcaccccg gacgatgcct
      601 gtggaacgac gcgctgtcat tcccgtcccg gcgacccacg tggtcgccgc cgggtcaatg
      661 cggcatcgcc gatccgcatg ccgggctgtt cccagggtcc ttcacttcgt tcaggatgac
      721 gaggtggaag gagttgtgca ggaagcatgt ggtgcctgag cggtccctcg acaagctcgg
      781 gaggacgttt ctgcggaggc gttgcgccgg aagcatgtgg tgcctgggcg gtccctcgac
      841 aggctcggga ggacgtttct gcggaggcgt tgtgcaggga gcctgaggat ggccaggcgg
      901 tccctcgacg agcttgggac gaggtgaaga ggattattcg ggaagcctgg atggagccat
      961 gcgactccgc gacgaccttg gggcactgac atactttccg gtggtggcgc tcggtccgat
     1021 cgccaagttc ctgatgcagc ggggaggatg agaccttgaa atgggatcgg gttccgccga
     1081 aggatgaccg ggatgtgccg atagcgggtg acgatggcac ctatccgcac gtcacggtgg
     1141 gtgaatccgg cagcggtcag gcacctgcgc gtggacggcg gggaagcctg cggatcctgc
     1201 tcggcgcggc cccgggcgtc ggcaagacct acgcgatgct gcgcgacgcc catcggctgc
     1261 gggatgaggg aaacgacgtc gtcatcggct tcatcgaaac ccatgggcgg caggaaaccg
     1321 aaaatcagat cggcgatctc gaggttgtgc cgcgggcaaa gatcatctac cagggtgtca
     1381 cgcttgagga gatggatacc gacgcgatcg ttcgtcgaca tccgaagtac gtcctcgtcg
     1441 atgagcttgc gcacaccaac gcgccgggat cgccgcgcga gaagcgttac gaggatgtcg
     1501 cctacctgcg cgatcgcggg atcaacgtca tcggcgccct caatatccag catctggcga
     1561 gcatgcagga tctcgttgcc ggaatcaccg ggatcgaggt gcgggagtcc gttcccgatc
     1621 acatcctgac cgacgcgacg gaggtccagc tcgtcgacct gccggtcgag gcgctgatcg
     1681 agcggatcga gcaaggcaag gtctacccgc ctgaacgttc gacgcgcgcg ctccagaact
     1741 tcttccaacc cggcaacctg aacgcgctcc gcgagatggc gctccggcgc acggccgagg
     1801 gtgtcgacga gcggctcgcc gacatgatgc tcggtcgcgc cgcgagtgcg acgggcgatc
     1861 tcgtgacgcc gaacgagcgg gtcgcggtcg tcgtcgagcc cgatgcggag tggggggatg
     1921 tcctccgcaa tgcctggcgt ctcgcaagct cgatgaaggc ggagctgatc gcgctgacga
     1981 tcgcgccgct cggattgctg agcgagtttt cctccgagcg ggcgaacaag ctccggcgac
     2041 agcaggagct ggcggaggat ctcggggcgc gggtggatgt ggtttccagc gatggcggcg
     2101 ggatggagga tgatgtcgcg gcgatcgtgc gcgccctgaa ggc
//
LOCUS       DASTMD010000539         1984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2372644, whole genome shotgun sequence.
ACCESSION   DASTMD010000539 DASTMD010000000
VERSION     DASTMD010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372644"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFQ54_07880"
     CDS             <1..197
                     /locus_tag="VFQ54_07880"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU0164948.1"
                     /translation="PWAIRAFLTFAVPWAFATTLPAQALTSGIAWWVVAGAVAFAGAA
                     LFLLRILWNHAVRLYASASS"
     gene            577..1311
                     /locus_tag="VFQ54_07885"
     CDS             577..1311
                     /locus_tag="VFQ54_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164949.1"
                     /translation="MKLKLFTVIALLTLICGLTLRGPMAVAAQDEGTPSADSDLDLSN
                     LDGLKSAVGRSYVGDVDAMMSALIPSGTPGADASTPDLSSLGLFSLTGVVAEFEKTSQ
                     AKDGFDKATDEITKSLESDDSEDAIKLEDAKVSGVGDQSKAFTGTMDEGGIPLNVFVI
                     TAQQDNFIYVGVGITFADDAKDGTSDFTKAMIDGKAGKGDGTFSEDGKSTGGLWDKFP
                     TDGDKVLQGLTVSADELLYPQQEGGE"
     gene            1609..>1984
                     /locus_tag="VFQ54_07890"
     CDS             1609..>1984
                     /locus_tag="VFQ54_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164950.1"
                     /translation="MQYAPLRGRRRGRKGLRFLSTATFLVLTFGSLLSVLASPTVSAD
                     DATPNVDMTPTAPSGSADLTVNLFDCPAGYDLSDPGANPAADCTEPANGATFTIDGQN
                     SGYFSQENTGDSVNGAVYFGGVP"
BASE COUNT          366 a          588 c          656 g          374 t
ORIGIN      
        1 acccgtgggc gatccgcgcc ttcctcacct tcgctgtgcc gtgggcgttc gcgacgacct
       61 tgccggcgca ggcgctgacc agcggcatcg cctggtgggt cgttgccggc gcggttgcct
      121 tcgccggtgc ggcactgttt ctcctgcgga ttttatggaa ccacgcggtg cggctctatg
      181 cgagtgcgag cagctagggg ggatccgaaa cggtggcaag ataaccggga gttggcgttg
      241 gggggagcct ggttcctcaa cggggaggcg ggacaggccg tccggactgc ggtcccgccc
      301 tacgaacgac cggctatacg acgagcgccg cattcgatcc caggcatgtg gcgatcgcat
      361 cggttttcgc tgcagcgtgc ccaaggaatg gcgacctcac ggcgaaacca gtgaggattg
      421 attcgaagcg gggcaggagc gttcgtctgt cgcgcggaac aaactccgcg tgacaggcgt
      481 ttgatgttcg tgcccgggac ctccggcggg gaggtcgggt atttctcgtg ggagatggac
      541 tccgtgagaa cgttgtagag ggaaggattc tgaacgatga agttgaagct cttcaccgtg
      601 attgcgctct tgacgctgat ctgcgggttg accttgcgcg gaccgatggc ggttgcggcc
      661 caggatgagg gaacgccatc cgctgattcc gacctcgatc tcagcaatct cgatggcctg
      721 aagtccgcag ttggccgcag ctatgtcggc gatgtcgacg cgatgatgtc ggcgctcatt
      781 ccttcgggaa cgccgggcgc cgatgcctcc acgccggatc tctcgagcct cggtctcttc
      841 tcgctgaccg gcgtcgtcgc cgagttcgaa aagacgagcc aggcgaagga tgggttcgac
      901 aaggcgaccg acgagatcac caagagcctc gagtccgatg attccgaaga tgcgatcaag
      961 cttgaggacg cgaaggtgag cggcgttggc gatcagtcca aggcgttcac cggcacgatg
     1021 gacgaaggcg gcatcccgct gaatgtcttc gtgatcacgg cccagcagga caatttcatc
     1081 tacgttggcg tcggcatcac cttcgcggac gatgccaagg acggtacatc cgacttcacg
     1141 aaggccatga tcgatggcaa ggccggcaag ggtgatggca ccttcagcga ggacggcaag
     1201 tcgaccggcg gtctttggga caagttcccg acggacggcg acaaggttct ccagggcctg
     1261 accgtcagtg ctgacgaact gctctacccg cagcaggaag gcggcgagta accgtcgcgc
     1321 aagcacgatc ctcgcgacaa tcgaacgtac ctcgctcgac gatgagcgac gtccagatga
     1381 gcccccggca acggcgccgg gggctcatcg cgttgctggg agatcgttcc ggcaacgagt
     1441 aacgggcgat tgcgtatgtc tgagtgaacc gttccctgga aatgagtagt cttactcatc
     1501 ccttcgcgag agcaattccc taagctgtgg gagaactggc cctccatgca tcgttggcga
     1561 ctggagcagg tcagcgattg gtcttgctca gcgaaggaag ttacgcgcat gcagtacgct
     1621 ccgctccgcg gcaggcgtcg tgggaggaag gggttgcgat tcctttcgac ggcgacgttc
     1681 cttgtcctga cgttcgggtc cttgctctcg gttctcgcgt cgccgaccgt ttcggcagat
     1741 gacgccacgc ccaacgtgga tatgacgccg accgcgccat cgggatcggc cgatctcacg
     1801 gtcaatctct tcgactgtcc cgccggctac gatctgagcg accccggggc gaacccggcg
     1861 gcggattgta cggaacccgc caacggcgcg accttcacga tcgacggcca gaacagcggc
     1921 tacttctccc aggagaacac cggcgacagc gtcaacggtg cggtctactt cggaggagtc
     1981 cccg
//
LOCUS       DASTMD010000540         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1033910, whole genome shotgun sequence.
ACCESSION   DASTMD010000540 DASTMD010000000
VERSION     DASTMD010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033910"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFQ54_07895"
     CDS             complement(<1..225)
                     /locus_tag="VFQ54_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0164951.1"
                     /translation="MSDDNDKDGRIAVLLADDHGIVRAGLRALLEAQPDMVVVAEAAD
                     GPTAISEARRLRPTVVVSDLSMPGGGLEAIR"
     gene            complement(222..1280)
                     /locus_tag="VFQ54_07900"
     CDS             complement(222..1280)
                     /locus_tag="VFQ54_07900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0164952.1"
                     /translation="MRARILSVPLFYKVLIANLVIVLLGAVVGTYVTTTVARNSQVPS
                     REGLVLIFLVVGVLVSGTVNIMVLRAAFQPLDTLEQVADAVRRGDLSARAHPITFSDP
                     QLARLAETFNDTLEMLQQDQAQLRQLASQVIRAQEDERKRVARELHDDTAQILFAQLL
                     RVTALKSSPEEDVRRMAEQLENMTVEAIEGVRRLALELRPPALDDLGLREALADLAQR
                     FSEQLGIPVLYHETGLRGRLHPDIELVLYRVAQEALTNSAKHAGATQIWVSIVRTEYA
                     VSLSIDDDGIGISSPSSDGKGLGLGLFGMEERVALVGGELIVRPLQPTGVGIVANIPI
                     SRSRDLNRLMSGERIRVP"
     gene            1428..1919
                     /locus_tag="VFQ54_07905"
     CDS             1428..1919
                     /locus_tag="VFQ54_07905"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEU0164953.1"
                     /translation="MASTSSFDIVSKFDRQELKNAVDQAEREIHQRYDLKDTKTELTL
                     TDSEITINTESEYSLSAVKDILESKVIGRKLSLKILDYEEAIDASGGRQRQVIKLKEG
                     IKDEIAKQITKRIRDEFKKVTPQIQGDAIRVQAKSRDDLQGVITLLRDAEYPVALQFE
                     NYR"
     gene            complement(1957..>2822)
                     /locus_tag="VFQ54_07910"
     CDS             complement(1957..>2822)
                     /locus_tag="VFQ54_07910"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0164954.1"
                     /translation="ETLVEVSKTVFRTSARNFLDLILMPRMSRRTVLRRMETSDADWK
                     LIETALERGNGVILVTGHIGCFDFLGQAFAAKGYPLTIVTGRTTSRFIFDGVTYLRSS
                     HGNTLVEPTPSGVRHSYRALRRNGITVIVTDRDFFQNGKPVEFFGKKTTLPPGAVRIA
                     RDTGATVVPVITKRLGRGRHRMSLLEPFTIEKTADVDRDIDEGMQKVATVLEECIREN
                     VDQWAMFQRVWPDQAPDPVRVFPVGSPLESPLLERVASALPERRTTPREKSPGVVARV
                     RRWLGLGSESR"
BASE COUNT          554 a          898 c          880 g          490 t
ORIGIN      
        1 gcggattgcc tcgaggccgc cgccgggcat cgaaagatcg gagacgacga ccgtcggccg
       61 tagtcttctc gcctcggaga tcgccgttgg cccgtccgcg gcttccgcca ccacgaccat
      121 gtcgggctgg gcctcgagca gcgcgcggag cccggcgcga acgatcccgt gatcgtccgc
      181 cagaagtacg gcaatacgtc cgtccttgtc gttgtcatcg ctcatggaac cctgatcctt
      241 tcaccactca tcaatcggtt gaggtctcgc gatcgcgaga tcgggatatt ggcgacgatt
      301 cccacaccgg tgggctgcag tggtcgaacg atcaactcgc caccgacgag cgccacccgt
      361 tcctccatcc cgaagagccc cagtccgaga cccttgccat cgctcgaagg gctggagata
      421 ccgatcccat catcgtcgat cgagagactc accgcatatt cggtcctcac gatcgagacc
      481 catatctgtg tcgcacccgc gtgtttcgcg gagttcgtca gcgcctcctg cgcgacgcga
      541 tagagaacga gttcgatgtc ggggtgcagc cgcccccgga gccctgtctc gtgatagaga
      601 accgggattc cgagctgttc cgagaatcgc tgcgccagat cggccagcgc ttcgcgcaga
      661 ccgaggtcat ccagggccgg tggtcgcagt tcgagcgcga gacggcgcac gccttcgatc
      721 gcctccaccg tcatgttctc gagctgctcc gccatccgcc ggacgtcttc ctccggggag
      781 gacttgagcg cggtaacgcg cagcagctgg gcgaagagga tctgggcggt gtcgtcgtgg
      841 agctcgcggg cgacccgttt tcgttcatct tcctgggccc ggatcacctg gctggccagc
      901 tgccggagct gcgcctggtc ctgctgcagc atctcgaggg tatcgttgaa cgtctcggcg
      961 aggcgcgcaa gttgcgggtc gctgaaggtg atggggtgag cgcgcgccga gagatcaccc
     1021 cggcggacgg catcggccac ctgctccagg gtgtcgagcg gctgaaacgc ggcacgaagc
     1081 accatgatat tgacggtgcc gctgacgagg acgccgacga ccaggaagat cagcaccagt
     1141 ccttcgcggg acggaacctg cgagttccgg gcgacggtcg tcgtcacgta tgtcccgacg
     1201 acggcgccaa gcaggacgat gacgagattc gcgatcagga ccttgtagaa gagcgggacg
     1261 gacagaatgc gcgcacgcac gggaggctcc agcgagggga agaaccgggg agacgaaatc
     1321 ctcgtcacgg gaagtgtact tgcccggccg acacgaccgg agatcggtga cgtgttgcgc
     1381 tttgttacaa tcctgctgct attgatgtgc gcaaaggagt acaagacttg gcatcgacat
     1441 cttcatttga catcgtctcg aagtttgacc ggcaggaact gaagaacgcc gtggatcagg
     1501 cggagcgcga gatccaccaa cgatacgacc tcaaggatac gaagacggag ctgaccctca
     1561 ccgacagcga gatcacgatc aatacggagt ccgaatacag cctttcggca gtcaaggata
     1621 tcctcgagtc gaaggtgatc ggtcgcaagc tctcgttgaa gattctggat tacgaagaag
     1681 ccatcgacgc ctccggcggt cgccagcggc aggtgatcaa gctcaaggaa ggcatcaagg
     1741 acgagatcgc gaaacagatc acgaagcgga tccgcgacga attcaagaag gtaacccctc
     1801 agatccaggg tgacgcgatc agggtccagg cgaagagccg ggatgacctt cagggggtca
     1861 tcacgctcct gcgcgatgcc gaatatcctg tcgccctcca gttcgagaac tatcgctaga
     1921 cccggcgtcg ccgcgttgcc gggtccggat gcccggctag cggctctctg atcccaaccc
     1981 gagccaccgc cgaacccgcg caaccacacc cggggatttc tcccgcggtg tggtccggcg
     2041 ttccggcaac gccgacgcca cccgctccag gagcggactc tcaaggggtg agccgaccgg
     2101 gaagacgcgg acgggatccg gcgcctgatc cggccagacg cgctggaaca tcgcccactg
     2161 gtcgacgttt tcccggatgc actcctcgag gaccgtcgcc accttctgca tcccctcatc
     2221 gatatcgcga tcgacgtcgg ccgtcttctc gatcgtgaac ggctcgagaa gcgacatccg
     2281 atgtcgtccg cgcccgagac gcttcgtgat caccgggacc accgtcgcgc ccgtgtcgcg
     2341 cgcgatccgc acggccccgg gcggtagcgt cgtcttcttg ccgaagaact cgaccggttt
     2401 gccgttctgg aagaagtcgc gatcggtgac gatgacggta atcccgtttc gtcgcaacgc
     2461 ccgatacgag tgccggaccc ccgaaggtgt cggttcgacc agcgtattgc catgcgaact
     2521 ccgcaggtac gtgacgccgt cgaagatgaa gcgcgatgtc gtgcgaccgg tgacgatcgt
     2581 cagcggataa cccttcgccg cgaacgcctg gcccaggaaa tcgaagcatc caatgtgacc
     2641 cgtgacgagg atgacgccgt tcccgcgctc aagagccgtc tcgatcaact tccagtcggc
     2701 atcggatgtc tccatccggc gcaacacggt ccgtcgcgac atccggggca tcaggatgag
     2761 atcgagaaag ttgcgcgcac tcgttcggaa gacggtcttt gagacctcga cgagcgtctc
     2821 ct
//
LOCUS       DASTMD010000541         1949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2642076, whole genome shotgun sequence.
ACCESSION   DASTMD010000541 DASTMD010000000
VERSION     DASTMD010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2642076"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFQ54_07915"
     CDS             <1..505
                     /locus_tag="VFQ54_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0164955.1"
                     /translation="PATRTQASWDLLVEATREILDVLPEGVSFCLEAWPQTVLYDIPT
                     FTRFYAEIDDPRVGMIFDPANLMTTDNYYATGDMIDATFDALGDRFIASHCKDIEWVS
                     GFSQTALKEVVPGRGSLDYPTFLRRAATLGHDLPLIVEHLGDPEDVIEASAYIRRQAE
                     ETGITIA"
     gene            1232..>1949
                     /locus_tag="VFQ54_07920"
     CDS             1232..>1949
                     /locus_tag="VFQ54_07920"
                     /inference="COORDINATES: protein motif:HMM:NF027404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEU0164956.1"
                     /translation="MSDLSAFYGPNAGYVLELYDRYVADPTSVDADTRQFFESFSPDI
                     LASVTNTAAVPGTTPVAPASVGLSEADVRKIAAAVDLANGIREYGHLAVQLDPLGSAP
                     HGAPELSPDFYGITDDDLTRIPASVVGGNLAAGAANAAEAIARLREAYTAVAGFDFDH
                     IQIAEERVWLRDAVESGRFNVEYDAAAKRKLLERLTQVEVFERYLHQTYLGQKRFSLE
                     GNDVLIPMLDRIIDDATADGA"
BASE COUNT          340 a          671 c          596 g          342 t
ORIGIN      
        1 tcccgcgacg cgaacccagg cgtcgtggga tctcctcgtc gaggcgacgc gcgagattct
       61 cgacgtgctg ccggaggggg tcagcttctg cctcgaggcg tggccgcaga ccgtcctcta
      121 cgacatcccg accttcacgc gtttctacgc cgagatcgac gatccccgcg tcgggatgat
      181 attcgatcct gccaacctga tgacgaccga caactactac gcgacgggcg acatgatcga
      241 cgccaccttc gatgccctcg gtgaccgctt catcgcctcg cattgcaagg acatcgagtg
      301 ggtctccggc ttctcgcaga cagcgttgaa ggaggtcgta cccgggcgcg gaagcctcga
      361 ctacccgacc ttccttcgtc gcgccgcgac cctgggccac gacctgccgc tgatcgtcga
      421 gcatctgggc gatcccgagg atgtcatcga agcgtcggcc tacatccgtc gccaggcgga
      481 ggagaccggc atcacgatcg catagcacgt ccacgcacgc ctttcacgtc atccccgcgc
      541 cgtcgcgccg ccgtcaggcg tgcgtccgta ggggcgaacc tggtgttcgc ccggtaccac
      601 gtcccatgcg cgatgcggcg cgtgcctgcc ccccccgggg aagcgtgtcg tggggaaaac
      661 acgcgggcgc caactggtcc ctcgacaggc tcgggaggac gtgtcttgta cggcgccgta
      721 ccggaagcac gtgctcactc ccgtccccac ggcgcttgtc gccgttcccg ggcggcggcg
      781 tggaggaagc gtgtggatgc cgtcaggcgc gggaagacgt gtcttgtgac gcgtcgggag
      841 gggagcaccc ggccgcaatc gggcaatctg cacagcagat tcggtcgcct cccgccgatc
      901 ctgccggagc tcccgacgac tcctcatgcg ctcctcccaa attgcctcgc attgtgaaaa
      961 tgatcacgca aacagcaggg caattcgcct gatttccgcc tgatctgcgc cagaatccaa
     1021 ccccgggttt cgtgacatct atgctatgct cttcaccgac gtacgggccg ggacagtgtg
     1081 gtcggcagag ccctcgttga ggttcctgtt gcgtgcaatc cccctttccc gactgtcgca
     1141 ccgtcgacgt gacggcgacg atgctcgtgt tgaggatggt cgtgaacatc gccatggtga
     1201 gcacgcatcc aaggagccaa cggacagacc aatgagcgac ctgagcgcgt tttacgggcc
     1261 aaacgcgggc tacgtgctgg agctctacga tcggtatgtg gcagatccca catcggtcga
     1321 tgctgatacc cggcaattct tcgaatcctt ttccccagac atcctggcaa gcgtcacgaa
     1381 caccgctgcg gtacctggca ccacgccggt cgcgcccgca tcggtcggtc tcagcgaggc
     1441 cgacgtccgg aagatcgcgg ccgctgtcga cctcgccaac ggcatccgcg agtacggcca
     1501 cctcgcggtt caactcgatc ccctcggcag tgcaccgcac ggggcgccgg aactctcgcc
     1561 agacttctat ggaataaccg atgacgatct gacgcgaatc cccgccagcg tggttggcgg
     1621 aaacctcgct gccggcgccg cgaatgccgc cgaggcgatc gctcgcctcc gcgaggccta
     1681 cactgcggtc gccggtttcg atttcgatca catccagatc gccgaagagc gggtctggct
     1741 gcgggacgcc gtcgagtccg gccgcttcaa cgtcgagtac gacgcggcgg ccaagcggaa
     1801 gctgctcgaa cgcctgaccc aggtcgaggt cttcgagcgc tacctgcatc agacctacct
     1861 cggccagaag cgattctcgc tcgaagggaa cgacgtcctc atcccgatgc tcgaccgcat
     1921 catcgacgac gcgacggctg acggcgcgg
//
LOCUS       DASTMD010000542         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_764850, whole genome shotgun sequence.
ACCESSION   DASTMD010000542 DASTMD010000000
VERSION     DASTMD010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764850"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(661..1578)
                     /gene="thrB"
                     /locus_tag="VFQ54_07925"
     CDS             complement(661..1578)
                     /gene="thrB"
                     /locus_tag="VFQ54_07925"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU0164957.1"
                     /translation="MQFRVKVPASSANLGPGFDAIGIALDLWNTVTIDTDGTAGDVTN
                     SGSEAFLLDGRENLTIKAMKTLARDYGKRLPAFSLQAETEVPIARGLGSSAAALVAGL
                     VAANYLLELNLDQEELFAHAWRIEGHGDNVGAALYGGAILAVPNVPYATRLWAGEERG
                     YTAVVFIPEVTGATWAARAALPNDIPHADAAFNVATASGLAVGLVIGDTKLIGAGMND
                     RLHEPYRARLFSHLNEMKATAREHGAIGACLSGAGPTVLAFVPHERVDAVIAAMRETS
                     VEIAVPGRVTELAPTACGATLVHDLVQTG"
     gene            complement(1562..>2406)
                     /gene="thrC"
                     /locus_tag="VFQ54_07930"
     CDS             complement(1562..>2406)
                     /gene="thrC"
                     /locus_tag="VFQ54_07930"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU0164958.1"
                     /translation="LASYAATAGIPAIVLVPEGKISLSKLSQTIAYGATVIQIDGDFD
                     RALKLLRELTDAYDVYLANSVNPYRPEGQKTIVFEMLDQLGWDVPDVIALPGGNLGNT
                     AAFGKALSEVYQVGLIAKMPRIVTVQAAGAAPFVAYYESGFDHYAPVHAETIASAIKI
                     GDPASIERARRSIETTNGIVTAVTDDEIMAAKAVIDLAGIGCEPASAASLAGIRKLVQ
                     RGELANDLTIVGVLTGHVLKDTDAIVGYHLTDEPRHALANRPVSVPAELDALKKVLDD
                     AVQG"
BASE COUNT          397 a          798 c          768 g          443 t
ORIGIN      
        1 ccgcgctcgt cgccgcttcg gatcggcgct gccgatccat ctcctgggcg cgacgcgcaa
       61 tgcggtgtcg gagtcggagt ttaccctgag cctgtcgacc gagccgggca tgcgtccaac
      121 gctgtcgcgt aaaggttcgt catttcgagc ctgtcgagaa atccccgtgc gaagcacatc
      181 cggcccgttc gacaggctca gggtaaactc cgccggaccc gaccacagcg attccctgca
      241 acatgcgctg cggaaacgga tcggctgcgc cgcattgacc cgaggcgacc acatgggtcg
      301 cgccgggacg agaacgaacc gcgcaccggg agcgccgacc gcgaccggtt ctccgccccg
      361 ctcggtttat gcgacagcgt tgggttttat gccccgccgc atagcgggtg ccccgtgcgg
      421 caggtgtcga ccgtttcggt tctgccgtag gaagcagctc gtatcgacat gcgcacgggg
      481 ctatgggtgg attcaccatg ttccgggatt gcgttcgtgg cgttcatatc gcgcttcgcg
      541 cgatgggtca gaccagctga cccgctacga aggcgtgctg cgggatgcat tcgtagggga
      601 gacagagaag tatccctagc gatgaaatcc ggaacctttg acaaccttga tgttggtcgg
      661 ttatccggtc tgcacgagat cgtgaacgag ggtcgcgccg caagcggtcg gggcgagttc
      721 tgtaacacga ccgggaaccg cgatctccac cgacgtctcg cgcatcgccg cgatcaccgc
      781 gtcgacacgt tcgtgcggga cgaaggcgag caccgtcggg cccgcaccgc tgaggcaagc
      841 cccaatcgcg ccatgctcac gcgccgtggc tttcatctcg ttgagatgcg agaagaggcg
      901 tgcccggtac ggctcatgaa gccgatcgtt catgccggcg ccgatcagct tcgtgtcgcc
      961 tatgaccagc ccgaccgcga gcccgctcgc cgtcgccacg ttgaacgccg cgtccgcgtg
     1021 cgggatgtca ttgggcagcg ccgcccgcgc tgcccaggtc gcgccggtga cttccgggat
     1081 gaaaacgacc gccgtgtatc ctcgctcttc gcccgcccag agtcgcgtcg cgtaggggac
     1141 gttcggaacc gcgaggatgg cgcccccgta cagcgcggcg ccgacattgt cgccgtgccc
     1201 ctcgattcgc caggcgtgcg cgaagagttc ctcctgatcg aggttcaact cgaggaggta
     1261 gttcgcagca accagccccg cgacgagcgc cgctgccgac gaaccgagtc cccgtgcgat
     1321 cggcacttcc gtctccgcct ggagcgagaa cgccggcagc cgcttgccgt agtcccgcgc
     1381 cagcgtcttc atcgccttga tcgtcaggtt ctcgcgacca tcgaggagga atgcctcgga
     1441 gccgctgttc gtgacgtcac cggcggttcc gtccgtatcg atggtgaccg tgttccagag
     1501 atcgagcgcg attccgatcg cgtcgaatcc gggaccgaga ttcgccgacg atgccggcac
     1561 cttaaccctg aactgcatcg tccagcactt tcttcaatgc atcgagttcc gccggcacgc
     1621 tgaccgggcg attcgcgagt gcgtgccgtg gctcatccgt caggtggtag ccaacgatcg
     1681 catccgtgtc cttcaggacg tggccggtca gcacgccgac aatcgtgaga tcgttcgcga
     1741 gctcgcccct ttggaccagc ttccggatcc ccgccagcga tgcggccgaa gcaggctcgc
     1801 acccgatgcc cgcaaggtcg atcaccgcct tcgccgccat gatctcgtcg tcggtgaccg
     1861 cggtgacgat gccgttcgtc gtctcgatgc tccgccgggc gcgctcgata ctcgcgggat
     1921 cgccgatctt gatcgcactc gcgatcgtct cggcgtggac cggcgcgtag tgatcgaagc
     1981 cggattcgta gtaggcgacg aatggcgcgg cgccggcggc ctgaaccgtc acgatccgcg
     2041 gcatcttcgc gatcagccct acctggtaga cctcggagag cgccttgccg aacgcggcgg
     2101 tgttcccgag attgccgccc ggcagggcga tcacatccgg gacgtcccaa cccagctggt
     2161 cgagcatctc gaagacgatc gtcttctggc cctcgggtcg gtagggattt acggaatttg
     2221 cgaggtagac atcataggcg tcggtcaact cgcggaggag cttcaacgcg cgatcgaagt
     2281 cgccgtcaat ctggatgacc gtcgcgccgt aggcgatcgt ctggctgagt ttgctgaggc
     2341 tgatcttgcc ttccggcacg agcacgatcg ccggaattcc tgccgtcgcc gcataggacg
     2401 ctagcg
//
LOCUS       DASTMD010000543         1822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_230338, whole genome shotgun sequence.
ACCESSION   DASTMD010000543 DASTMD010000000
VERSION     DASTMD010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230338"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFQ54_07935"
     CDS             complement(<1..559)
                     /locus_tag="VFQ54_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0164959.1"
                     /translation="MSSARYRAAVIGCGKMSRGHSRSYVNSPRVDLVAGVDVFAGARD
                     AFSTEFGVATTYEDPAEMLEREKPDIVSVCTWPPLHADLTVAAFEAGVKAVWCEKPMA
                     VHMEDAERMVAAAKRAGGVLVINHQRRYVERYNRARALIDAGEIGDVTQITGIAGGDN
                     LTDGTHLIDMTRYLLHDTPISKVFGA"
     gene            780..1748
                     /locus_tag="VFQ54_07940"
     CDS             780..1748
                     /locus_tag="VFQ54_07940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0164960.1"
                     /translation="MRRLRILTWPIHGSYFTALATLDHEWFLPVKPGAPEGYGGRGRA
                     PFPPNIHDVPAASVRDLELDLVLLQSVRNLEQDAPDLLGPTQRGLPTIYLEHNTPFPD
                     PVSSRHPFGDRRGLLVHVTRFNQLMWDNGDLPTRVVEHSVAIDPKVAYTGRRAEGITV
                     INSMPRRGRKVGLDLLQAARRHVPIQLAGFENEGLEGLGDIPYPDLHRVVADFRFLFS
                     PCRYTSLPLAVIEALTIGMPVVALATTELPDVIVNGVHGYVSNDPEVLIRAMRRLIDD
                     PEHARELGANARALAAERFGLARFARDWNDAFEAAIDLAGSSPRES"
BASE COUNT          314 a          603 c          560 g          345 t
ORIGIN      
        1 tcgccccgaa gaccttcgag atcggtgtgt cgtgcaggag gtagcgcgtc atgtcgatga
       61 ggtgcgtgcc gtcggtcagg ttgtcgccac cggcgatgcc ggtgatctgg gtgacgtcgc
      121 cgatctctcc cgcgtcaatc agcgcccgcg cccggttata ccgctcgacg tagcgccgct
      181 gatgattgat gacgaggacg ccgcccgccc gcttcgccgc ggcgaccatc cgctccgcgt
      241 cttccatatg aacggccatc ggcttctcgc accagacggc cttgaccccg gcctcgaacg
      301 ccgcgacggt gagatcggca tggagtggtg gccaggtgca gacgctgacg atgtcgggct
      361 tctcgcgttc cagcatttcc gctggatcct cgtaggtcgt ggcgacgccg aactcggtgg
      421 agaatgcgtc acgcgcgccg gcgaagacat cgacacccgc gaccaggtcg acccggggtg
      481 agttcacgta ggaacgggaa tgaccgcggc tcatcttgcc gcaaccaatg acagccgcac
      541 gatatctggc actggacatc gggacctcct tcatatcgga tatcgataat cgacaagatg
      601 gtagcagccg gttgccttgt caatagcggg gcaccctacg ctcgcgcacg gttcctcgtc
      661 aacaaccggg cgtcgcgttc ctggcatcgt tggtgcattt gggaagctgc gccggttgcc
      721 ccggcatcag gacggttcga cctcgaacgt ccatgatccg cgacctcctg aggattgcca
      781 tgcgtcgcct gcgaatcctg acctggccga ttcacggaag ctatttcacc gcgctcgcga
      841 ccctcgatca tgaatggttt ctccccgtga aaccgggtgc tccggaagga tacgggggac
      901 gtggtcgcgc gccgttccct ccgaacatcc acgatgtgcc tgccgcctcc gtccgcgatc
      961 tggagctcga tctcgtgctc ctccaatccg tccggaatct cgaacaggat gcgccggacc
     1021 ttctcggtcc gactcagcgg ggacttccga cgatctatct ggagcacaac acaccgttcc
     1081 cggatccggt ttccagccgg catccgttcg gcgaccgtcg cgggctgctc gtccacgtca
     1141 cgcgcttcaa ccagttgatg tgggataacg gcgacttgcc gacgcgggtc gttgaacaca
     1201 gcgtggcgat cgatccgaag gtcgcgtaca cggggcgccg tgccgagggc atcacggtga
     1261 tcaacagcat gccgcgccgt gggaggaagg tcggactgga tctgcttcag gccgcacggc
     1321 gtcacgtccc gatccagctc gccggcttcg agaacgaagg actcgaaggt ctcggcgaca
     1381 tcccgtatcc cgaccttcac cgcgtcgtgg cagatttccg gttcctcttc agcccctgcc
     1441 ggtatacgtc gctgccgctg gccgtgatcg aggcgttgac gatcggaatg cccgtcgtgg
     1501 ccctggcgac gacggaattg ccggacgtca tcgtcaacgg tgtccatggc tacgtctcga
     1561 acgatccgga ggtcctcatc cgcgcaatgc gtcgcctgat cgacgatccc gaacacgcgc
     1621 gcgaactcgg cgcgaatgct cgcgcactgg ccgccgagcg cttcggtctc gcccggttcg
     1681 cccgggactg gaacgacgcg ttcgaagccg cgatcgatct cgcggggtcg tccccgcgcg
     1741 aatcttgatc tgaacaggca tcacaggagg agatatcgaa atggaccaga ctccgattca
     1801 cgacaaggtc gccttcgtca cc
//
LOCUS       DASTMD010000544         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3177610, whole genome shotgun sequence.
ACCESSION   DASTMD010000544 DASTMD010000000
VERSION     DASTMD010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3177610"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1040)
                     /locus_tag="VFQ54_07945"
     CDS             complement(213..1040)
                     /locus_tag="VFQ54_07945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU0164961.1"
                     /translation="MSRPPRIGVHIWPGGAPSYASWRGAVLEAEDMGADAVFGYDHFH
                     KPFVEIAPEGPVLLPEQPDVNNFESWTALASWGEATSRVEIGLLVTGIGYRNPELLAD
                     MARTVDHISGGRLILGLGSGWYEKDYAVYGFEYGTVASRMELFAEGLARIKHRLAALT
                     PKPLREIPILIGGSGEKKTLPLVGQYAGIWHSFLDLETFRRKNQLVHDHATAAGRDDA
                     EIERAIAWQGPAMADAFVAEGASLFTMEIKPTSDGYDLSPVREMLAWRDAQLGKGGR"
     gene            complement(1100..1858)
                     /locus_tag="VFQ54_07950"
     CDS             complement(1100..1858)
                     /locus_tag="VFQ54_07950"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0164962.1"
                     /translation="MAATLSDLTGTTSVVTGATSGIGRATAIALAARGSHVLVAGRDT
                     ARGTEVVETIRAAGGTADFITADLHDAASAGEFASRAVALGGGHVDILVNNAGIAPYL
                     PTTGTSEDVFDDVFAVNVKVPFFLVAEFAPSMAERGKGAIVNLSSMVGEYGDPNYGAY
                     GASKSAINLLTKSWAAEFGPRGVRVNAVAPGPTRTPGTAPYGDGLDQFAALAPAGRVA
                     APEEIAAAITYLVSDEASFVFGAILPVDGGRTAA"
     gene            2105..>2713
                     /locus_tag="VFQ54_07955"
     CDS             2105..>2713
                     /locus_tag="VFQ54_07955"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0164963.1"
                     /translation="MANTIEIQGQRLTPKGEATRERIVAAAANLMNERGFAGTSTEDV
                     KSAAGVSSSQVYHYFSDKKSLLRAVIAYQTDLVLGVQEPFLAKLDSIEALEAWRDFMV
                     DYQNQRHCVGGCPMGSLAAELVERDADARADFVVGFDRWVAGIRDGLQSMQEHGHLHA
                     DVDIERLAQAFLAAIQGGLFLSQTNRNTIALKAVLDELIDRVR"
BASE COUNT          510 a          792 c          883 g          528 t
ORIGIN      
        1 caccggccgc ctcgagctgt gcctgctctg ctgcccaggc gcgcatcgcg tcgtccatgg
       61 gctgatcggc gcgactggcg tgcatggtgg cgacttgttg cgcgatggtc tcggtgacgt
      121 tggacatgat cgttcctttc aagtggtcag gcggagtgag tacagaaggc aaggatcaag
      181 cggtggtggc gatgtgaggt ccgttagtcc gatcaccgac ctcctttgcc gagttgagca
      241 tcccgccagg caagcatctc gcggacgggg gagaggtcgt acccgtcgct tgtcggcttg
      301 atttccatcg tgaagaggga tgcaccttcc gcgacgaacg catccgccat cgcgggacct
      361 tgccaggcga tagctcgctc gatctccgcg tcatcgcgtc ccgctgcggt ggcatggtcg
      421 tgaacgagtt gattcttgcg acggaatgtc tcgagatcga gaaagctgtg ccagataccc
      481 gcatattggc cgacgagtgg aagcgtcttc ttctcgcccg aaccgccgat caggatcggg
      541 atctcccgta gcggtttggg cgtcagcgcc gccagccgat gcttgatgcg agcgagcccc
      601 tcggcgaaga gctccatgcg tgacgcgacg gttccatact cgaagccgta gacggcgtaa
      661 tccttctcgt accagccaga acccaatccc aggatgaggc ggccgccact gatgtggtcg
      721 acagtacgtg ccatgtcggc gaggagctcg ggattccggt atccgatgcc ggtgacgagc
      781 aacccaatct cgacccgact ggtggcctcg ccccatgagg cgagtgccgt ccaactctcg
      841 aaattattga catctggttg ctctggaagc agtaccggcc cctcgggtgc gatctcgacg
      901 aatggcttgt ggaagtggtc gtacccaaag acggcgtcgg cacccatgtc ctcggcttcg
      961 aggaccgctc cccgccagga cgcgtagctc ggcgcaccgc cgggccagat gtgaacgcca
     1021 atgcgaggcg gacgactcat gtgaagcaac tcctggggat cggccccgta tttgaacgat
     1081 gacggaatgg ctcggacgat caggcggcgg tgcgaccacc atcgactggg aggatcgcgc
     1141 cgaagacgaa gctggcctcg tccgagacga ggtaggtgat tgcggcggcg atctcttccg
     1201 gcgctgcgac gcggccggct ggcgcgagtg ccgcgaactg gtcgagacca tcgccgtagg
     1261 gcgcagttcc cggtgtccgg gtcggacccg gtgcaacggc gttgacacgc acgccgcgcg
     1321 ggccgaactc cgctgcccac gacttggtga ggagattgat cgcggacttg cttgccccat
     1381 aggcgccgta attcgggtca ccgtattcgc cgaccatcga actcaggttc acgattgcgc
     1441 ccttgccgcg ttccgccatc gagggtgcga attctgcgac gaggaagaag gggaccttca
     1501 cgttcacagc gaagacatca tcgaagacgt cttcactggt gccagtggta ggcagatagg
     1561 gtgcgattcc ggcgttgttg acgaggatgt ccacgtggcc accaccaagc gcaaccgccc
     1621 gggaagcgaa ttcgcccgcc gaggcggcgt catgaagatc cgcagtgatg aaatccgccg
     1681 taccgccggc ggcgcgaatc gtctctacga cctctgtccc tcgcgctgta tcacgcccgg
     1741 caacgaggac gtgcgacccg cgcgcggcca aagcgatcgc cgtggcgcgc ccgatgccgc
     1801 tggttgctcc tgtgacgacg gatgtggttc ccgtgagatc cgagagagtt gctgccatag
     1861 ctgtaccctt caataatcat acagatcgtt ctggattgaa cgatccatat tctggcattg
     1921 aaattatgga ttgtcaattc cagaattaga acggcgtggc tgacgatcgt gtctggtggt
     1981 cgggacgatc cggtgaggac gccggatcgg agtcgggcaa ccgcaatctc gcagtagaat
     2041 gggctcgttg gcgaacgccg gaaatatgat cgatgggcat gcgttggaat ctactggtga
     2101 tgaaatggcg aacacgatcg agatccaggg tcagcgcctg accccgaagg gtgaggcaac
     2161 ccgcgagcgg atcgttgccg cagccgcgaa cctgatgaat gagcgcgggt ttgccggaac
     2221 gagcacggag gatgtgaagt ccgcggccgg tgtcagcagc tcgcaggtct accattattt
     2281 ttccgacaag aagtcgctct tgcgggcggt gatcgcctac cagaccgatc tcgtgcttgg
     2341 ggttcaggag cctttcctcg cgaagctgga tagcatcgag gcgctcgaag cgtggcgcga
     2401 tttcatggtc gattaccaga atcagcgtca ttgcgtcggt ggatgcccga tgggctcact
     2461 cgcggccgag cttgtggaac gcgatgctga tgcccgtgcc gactttgtcg tcgggttcga
     2521 tcgttgggta gcggggatcc gggatgggct tcaatcgatg caggaacacg ggcacctgca
     2581 tgccgacgtc gatatcgagc ggctggcgca agcattcctc gcggcgattc agggtggact
     2641 ttttctcagc cagaccaacc gcaacacgat cgccttgaag gccgtgctcg acgaactgat
     2701 cgatcgcgtt cgc
//
LOCUS       DASTMD010000545         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_944652, whole genome shotgun sequence.
ACCESSION   DASTMD010000545 DASTMD010000000
VERSION     DASTMD010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944652"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFQ54_07960"
     CDS             <1..447
                     /locus_tag="VFQ54_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0164964.1"
                     /translation="DSIANRLQLQSTYPDKSLGRSVPYQVTRVPWGFGMMYIAGIVSG
                     LLGIGSGALKVLAMDTIMKLPIKVSSATSNFMIGVTAAASSGIYFWKGDIVPEIAAPV
                     AIGVLCGATIGARFLSRFRSVTIRQAFIVVMAIVAIEMLLRGFGIG"
     gene            444..869
                     /locus_tag="VFQ54_07965"
     CDS             444..869
                     /locus_tag="VFQ54_07965"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEU0164965.1"
                     /translation="MSNVPLTADQTRPQTPGTGRSMEDTISIVLRIGVMIAGAVILLG
                     LVCTFIPALDRGSGSTIHELLDNGGSQIDVSPATIAKGLGKFDPIAIIQFGLLLLVLT
                     PIVRVAMTLFLFIERKETIFIAITTIVLFILLLGFLGII"
     gene            complement(1399..>2233)
                     /locus_tag="VFQ54_07970"
     CDS             complement(1399..>2233)
                     /locus_tag="VFQ54_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0164966.1"
                     /translation="DEQPEIDAPGAVAVTELRGEVVFDDVSFAYTADRPTLEHVSFRA
                     APGQTIALVGPTGAGKTTIVNLLTRFYEIDRGEITIDGRDIRKIQKEELRRQLGIVLQ
                     DTFLFAGTVADNIRYGRLNATDEDVRLAAQTANADEFILRLSHGYDTEITERGGNLSQ
                     GQRQLVAIARAVIANPRILILDEATSSVDTRTERHIQDAMLRLMQGRTSFVIAHRLST
                     IRQADLILVVNHGQIIERGTHDELRALNGFYERLYSGQLREPLALEIEEFVASVEPTD
                     D"
BASE COUNT          368 a          750 c          664 g          451 t
ORIGIN      
        1 gactccattg cgaatcgcct gcagctgcaa agcacctacc ccgacaagtc attgggccga
       61 tcggttccct atcaggtgac gcgcgttccc tggggtttcg gcatgatgta catcgccggc
      121 atcgtctccg ggttgctcgg catcggctct ggcgccctga aggtcctggc gatggacacg
      181 atcatgaagc tgccgatcaa ggtctcgagc gcaaccagca acttcatgat cggcgtgact
      241 gcggcggcaa gttccggaat ctacttctgg aagggtgata tcgtgcccga gattgcggcg
      301 ccagtcgcga tcggcgtgct ctgcggcgcc acgatcggcg cccgtttcct ctcccggttc
      361 cggagcgtca ccatccgcca ggcgttcatc gtcgtgatgg cgatcgtcgc catcgagatg
      421 ctgctgagag gattcgggat cggatgagca acgtgccgtt gaccgcggat cagacgagac
      481 cccagacgcc gggtaccggt cgctcgatgg aggacacgat cagcatcgtc ctccgcatcg
      541 gcgtcatgat cgccggcgcc gtcatcctgc ttggactcgt ctgcacgttc attccggcgc
      601 tcgaccgggg cagtgggagc acgattcacg agttgctcga caatggtggc tcacagatcg
      661 atgtctcccc ggccacgatc gcgaagggac ttggcaagtt cgacccgatc gcgatcatcc
      721 agttcggact gctcctgctc gtcctcactc cgatcgtccg cgtcgcgatg acgctcttcc
      781 tcttcatcga acgcaaggaa acgatcttca tcgcgatcac gacgatcgtg ctgttcatcc
      841 tcctgctcgg cttcctcggc atcatctagc cgagaccggg aggcaagacg ccaacggcgg
      901 cgccgggcgg ccctgacttc cgcgtccaaa cgaaatccag aacggtcacc agtcgtctgt
      961 caccggtgtc ttgctggtcg gcaccgtagt aagccaacga tcatattcgg cctcgccgca
     1021 cgggccacgc ccttcggcga acgcagggca ggctcgttgg tggcccgctc cgagaccagc
     1081 gtggcaattc agctgtgaca ggtctggtgc cgccggggtt ggtagggcgg ggttttatac
     1141 cccgtcgaaa tctctcggtt gctcactgcg cgtcccgttc tcgtccccac ggcgcttgtc
     1201 gccgttccgg atcggcgctg ccgatccgcc tcttcccccg tgccgactcg cccttcgcgc
     1261 atttgcccgc gcccgctgat gcgatcgatg ggagtcgcgc agacactcgg gacacgaatt
     1321 cgtgtcccca catctatagg gatatgttgc gcaaaatgtg ctcgacaaga aggcctatgc
     1381 cgccatcgcc atcctccgtc agtcgtccgt gggctcgacc gacgcgacga attcctcgat
     1441 ctccaacgcg agcggctccc ggagctgacc gctgtagagc cgctcgtaga atccgttcag
     1501 agcgcgcaac tcgtcatggg ttccgcgttc gatgatctgc ccgtggttca cgacgagaat
     1561 caggtcggcc tgtcgaatcg tgctcagccg gtgggcgatg acgaagctcg tccttccctg
     1621 catcaacctc agcatcgcgt cctggatgtg tcgctccgtc cgcgtgtcga cactactggt
     1681 cgcctcgtcg agaatcagga tccgcggatt cgcgatcacg gcccgtgcga tcgccacgag
     1741 ctgccgctgc ccctggctca ggttgccccc gcgctccgtg atctccgtgt cgtacccgtg
     1801 cgacagccgc aggatgaact cgtccgcatt ggccgtttgc gccgccaggc ggacatcctc
     1861 gtcagtcgca ttcagccgac cgtaccggat gttgtcggcg acggtccccg cgaacaggaa
     1921 cgtgtcctga aggacgatcc cgagttgccg ccgcaactcc tccttctgga tcttccggat
     1981 gtcccgccca tcgatcgtga tctcaccacg atcgatttcg tagaaccgcg tgaggagatt
     2041 gacgatcgtc gtctttcccg ccccggtcgg gccgacgagc gcaatcgttt gcccgggcgc
     2101 cgcgcggaag ctcacgtgct cgagcgtcgg acggtccgcg gtataggcga acgagacatc
     2161 gtcgaagacc acctcgccgc gcaattcagt tacggcgacg gcgccgggcg cgtcgatctc
     2221 gggctgctca tcc
//
LOCUS       DASTMD010000546         1744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_765176, whole genome shotgun sequence.
ACCESSION   DASTMD010000546 DASTMD010000000
VERSION     DASTMD010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765176"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VFQ54_07975"
     CDS             complement(<1..770)
                     /locus_tag="VFQ54_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0164967.1"
                     /translation="MPPTVSSDEVLSLLATQFTSAIDNLVQLPGGGVARIFAFDTAGG
                     EYVVRFNSAAELDFDNEAAIIDLLAGSGVPIPRMIAHGVIRDLSWAISDRIPGVQLNS
                     LPPDEFAAMTPQVFATLDRIHATDITTTAGYGYFDATLTGRSSSWREHVLSIREDHPG
                     GYNPPWEVTFATTALDRSLVMSLFAEIERLVAFCPEGRWLIHGDYGYDNILVQDGKIT
                     AVLDWLSARFGDFLFDVASLDFWSDFDFAVQFHQHYRES"
     gene            complement(983..>1744)
                     /locus_tag="VFQ54_07980"
     CDS             complement(983..>1744)
                     /locus_tag="VFQ54_07980"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEU0164968.1"
                     /translation="EEIATLGLADRAVVDGRCPVILPEHIAAEQGDTNLSEHVGSTKT
                     GNGHARSDFVLRRGPQAKDIPELAPYIADVAKEANTRAATEEIIIQGSQGTFLSLALS
                     PDYPFTTSDNCTVAACIDDVGLSWRLVKDAVMIVKVMPSRVGQGPLPFEMTEDEILAR
                     GIAERGVVTGRIRRKASKIDPELLAYATMLNGPTQIVLTFCDHLDPDMKGVTHRDGIT
                     RPVLDVIAEVEKISGVPVTMVDTGKYLDDLVEIPQ"
BASE COUNT          340 a          529 c          572 g          303 t
ORIGIN      
        1 gactcgcggt agtgctgatg aaattgcacc gcgaaatcga aatcggacca gaagtcgagg
       61 ctggcgacgt cgaagagaaa gtcgccaaag cgggcgctga gccaatcgag gaccgccgtg
      121 atctttccgt cctgaacaag gatgttgtcg taaccgtagt ctccgtgaat cagccagcgc
      181 ccttccgggc agaaggcgac gagccgctcg atctcggcga agagcgacat gaccaacgac
      241 cggtcgagcg cggtggtggc gaatgtcacc tcccagggcg gattgtagcc acccggatga
      301 tcctcccgaa tcgagaggac gtgctcgcgc cagctcgaag aacggcccgt aagcgtggcg
      361 tcgaagtagc catagccggc ggtcgtcgtg atatcggtcg cgtgaatgcg atcgagggtg
      421 gcaaagacct gtggggtcat cgccgcgaat tcgtccggtg ggagtgaatt cagctggacg
      481 cccggaatcc gatcggagat cgcccaggag agatcgcgta tgacaccgtg cgcgatcatc
      541 cgggggatcg gcacgccact gccggcgagc agatcaatga tcgcggcttc gttgtcgaag
      601 tcgagctcag ccgcggaatt gaagcggacg acatactccc ctccggccgt atcgaacgcg
      661 aagatccggg cgacaccccc gccggggagc tgcacgaggt tgtcgatggc gctcgtgaat
      721 tgggttgcga ggagtgaaag gacctcatcc gatgaaacgg ttggcggcat cggtggctcc
      781 gtcttctgcg gaaacggtga tttgaaaacc ccgggggttg gcagggacgg acgttatgtc
      841 cgtccacaaa cgcgagcgtc agaaccacac gccaacgccc tccgggcaaa gcgtcgtcca
      901 tggaacggtc atatcgggct ccgcccgatg ggtcacacgg ggtgacccgc cacgaaaagc
      961 gatcgtcggt gactcagtga aactactgcg ggatttcgac gagatcgtcc aggtatttgc
     1021 cggtatcgac catcgtcacc ggcaccccgg aaatcttctc gacctcggcg atcacgtcga
     1081 gcacgggccg cgtgatgccg tcgcgatgcg tcaccccctt catgtccgga tcgagatgat
     1141 cgcagaaggt gagcacgatc tgggtcggtc cgttcagcat cgtcgcgtag gcgaggagtt
     1201 ccggatcgat cttcgacgcc ttgcgccgga tgcgcccggt gacgacaccg cgctcggcga
     1261 tgccgcgggc gagaatctcg tcctcggtca tctcgaacgg gagcggtccc tggccgacgc
     1321 gcgacggcat gaccttgacg atcatcacgg catccttcac caggcgccag ctcagtccca
     1381 catcgtcgat gcaggcggca accgtgcagt tgtcggaagt cgtgaacgga taatccgggc
     1441 tgagcgccag cgagaggaat gtcccctgcg acccctggat gatgatctcc tcggtcgcgg
     1501 cgcgggtgtt cgcttccttc gcgacatccg cgatgtacgg cgcgagctcc gggatgtcct
     1561 tcgcctgggg accgcgacgg agcacgaagt cggatcgggc gtgcccgttg cccgtcttcg
     1621 tcgagccgac gtgctcactg agattggtat cgccctgctc ggcagcgatg tgctccggca
     1681 ggatcaccgg gcagcgacca tcgaccacgg cacgatcggc gagaccgagc gtcgcgatct
     1741 cctc
//
LOCUS       DASTMD010000547         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2195058, whole genome shotgun sequence.
ACCESSION   DASTMD010000547 DASTMD010000000
VERSION     DASTMD010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195058"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFQ54_07985"
     CDS             complement(<1..827)
                     /locus_tag="VFQ54_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164969.1"
                     /translation="MSNATVQARDNEGVTIAGAATASAPKRRNLLQVLRRQKLAMTGA
                     TIVGFFIIVAIIGPFVAPHGDTQQFPDRQLKGPSADFIFGNDEFGRDVFSRILYGARI
                     SFQVGIVAVGISSVIGIFLGLIAGYFRGWADNILSLFMDVLYAFPTILLAIAIMTTLG
                     NSLINVMIAIGLVNVPTFMRVVRGSVLSVRNMTYVEAARAVGAPTRRIMIFHIFPNIT
                     GPLIVHASLNFAFAVLSEASLAYLGLGNRPPSPSWGSMVSSSYGFLQLAPWAALFPGA
                     "
     gene            complement(894..1844)
                     /locus_tag="VFQ54_07990"
     CDS             complement(894..1844)
                     /locus_tag="VFQ54_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0164970.1"
                     /translation="MLKFIIGRVLLLIPVLLGVSLVTFIIVRSIPGNPVETLLGADRH
                     TTPEQIAVIRHSYGLDDPLPVQYLKWLQHIVTGDLGKSLRTRRPLTTELGLRLPVTVE
                     LTIFAAILGGIPALLIGVLAALRRNSKFDWLATMGTLIGISMPNFLLATVLVLIFSYK
                     LKWLPPVGFTTFSSNPVQNLKTMILPAISLGLPFAAILMRITRSSVLEVLGQDYVRVA
                     RAKGLGQSRVVFRHVLPNAGIPILTVAGIQVAALLGGTVIIEQIYGLPGVGKYIYDSI
                     ANRDYPVVQSVTLVMATILVVVSVIVDILYAVLDPRLRTR"
     gene            complement(1911..>2497)
                     /locus_tag="VFQ54_07995"
     CDS             complement(1911..>2497)
                     /locus_tag="VFQ54_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164971.1"
                     /translation="VGLYPDTYWAGYKGDIPAQDLDGAKALLAKVDLPDGFKPKLLTW
                     QAYDFLSSTSVVVQEQLKQLGVESEIDPQENAIYLENYYAGDFDIAVMGASGYIDPND
                     WLEQSLETGGSSNAAGYSSAEMDALIAKGLETAKQEDRVSVYQKVQQLLIDDLPWISL
                     YNSQSFVGLNNKVQGFEFYLSSSFYALRETWLKH"
BASE COUNT          507 a          781 c          778 g          431 t
ORIGIN      
        1 gctcccggga aaagcgccgc ccacggggcc agttgcagga agccgtacga gctggaaacc
       61 atcgatcccc acgacggcga cggcgggcga ttgccaagac cgagataggc aagcgacgcc
      121 tcggaaagca cggcgaaggc gaagttcagc gacgcgtgaa cgatcagcgg ccccgtgatg
      181 ttcgggaaga tgtggaagat catgatccgc cgcgtcggtg ctcccacggc acgcgccgcc
      241 tcgacgtacg tcatgttccg cacggacagc accgaccccc ggacgacgcg catgaacgtc
      301 ggcacgttca ccagcccgat cgcgatcatc acgttgatca gcgagttccc caacgtcgtc
      361 atgatcgcga tcgcgagcag aatcgtcggg aacgcataaa gcacgtccat gaagagcgag
      421 aggatgttgt ccgcccagcc gcggaaatac ccggcgatca gacccaggaa gatcccgatc
      481 acggacgaga tgcccacggc aacgatcccg acctggaacg agatccgggc accgtacagg
      541 atacggctga agacatcgcg cccgaactcg tcgttgccga agatgaagtc ggcgctcggc
      601 cccttcaact gccggtccgg gaactgctgc gtatcgccat gtggcgccac gaagggcccg
      661 atgatcgcga cgatgatgaa gaatccgacg atcgtcgcgc ccgtcatcgc gagcttctgg
      721 cgacgcaata cctggagcag gttccgtcgc ttcggcgccg acgcggtcgc cgcgccggcg
      781 atcgtcactc cctcgttgtc ccgtgcctgt accgttgcgt tactcatggt tggttccgcc
      841 tgccctgctc ttcctggagc gcgttgcgtc cgacgcccga cgtcgtgatg tcatcatcgc
      901 gtccgcaatc ggggatcaag cacggcgtag agaatatcga cgatcacgct cacgaccact
      961 aagatcgtcg ccatgacgag cgtcacgctc tgcaccaccg gatagtcacg gttcgcgatc
     1021 gagtcgtaga tgtacttgcc aaccccggga agaccgtaga tctgctcgat gatgacggta
     1081 ccgccgagga gcgccgcgac ctggatcccg gccaccgtca ggatcggaat gccggcattg
     1141 ggcaggacat gacggaagac gacgcggctc tggccgagtc ccttcgcccg cgcgacgcgg
     1201 acgtagtcct gcccgagcac ctcgagaacg gacgatctcg tgatccgcat caggatcgcc
     1261 gcgaagggga gcccgaggct gatcgccggc aggatcatcg tcttcaggtt ctggactggg
     1321 ttgctcgaga acgtggtgaa gcccacgggc ggtaaccact tcagcttgta cgagaagatc
     1381 aggaccagga ccgtcgcgag gaggaagttc ggcatcgaga tgccgatcag ggttcccatc
     1441 gtcgccagcc agtcgaactt cgagttgcgg cgcagcgcgg cgaggacgcc gatcagcagc
     1501 gcggggatcc ctcccaggat ggccgcgaag atcgtcagct cgaccgtcac cggcaagcgc
     1561 aaaccgagct cggtcgtcaa cggacgccgc gtgcggaggg actttccgag atcgccggtc
     1621 acgatgtgtt gcagccactt caggtactgg accggaagcg gatcatcgag cccgtaggag
     1681 tgacggatca ccgcgatctg ttcgggcgtc gtgtggcgat ccgcgccaag cagcgtctcg
     1741 acgggattgc ccggaatcga gcgcacgatg atgaaggtga caagcgagac accaagcagc
     1801 accgggatga gcaacagaac gcgtccgatg atgaatttca gcatgagttc acctgatgtg
     1861 tgcgctcatc atcgaacgcg ttgccaaccc gtggtgccgc tcggtcgcgg ctagtgtttg
     1921 agccaggtct cccggagcgc atagaagcta gaggaaaggt agaactcgaa tccctgaacc
     1981 ttgttgttca gtccgacgaa cgactgggag ttgtacaggc tgatccaggg cagatcgtcg
     2041 atcagcaatt gctgcacctt ctgatagaca gatacgcggt cttcctgctt cgcggtctca
     2101 agacccttcg cgatcagcgc gtccatttcc gcactcgaat acccggcggc gttgctcgat
     2161 cccccggtct cgaggctctg ctccagccag tcgttgggat cgatgtaccc cgacgcgccc
     2221 atcacggcga tgtcgaagtc acccgcgtag tagttctcga gatagatcgc gttctcctgc
     2281 ggatcgatct cgctttcgac gccaagctgc ttcagctgct cctggacgac gaccgaggtg
     2341 ctggagagga agtcgtatgc ctgccacgtg agcagcttcg gcttgaagcc atccggcaga
     2401 tcgaccttcg cgaggagcgc cttcgcgccg tcgagatcct gggcgggaat gtcgcccttg
     2461 tacccggccc agtaggtatc cgggtagagc ccgacca
//
LOCUS       DASTMD010000548         2909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2997752, whole genome shotgun sequence.
ACCESSION   DASTMD010000548 DASTMD010000000
VERSION     DASTMD010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2997752"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFQ54_08000"
     CDS             complement(<1..598)
                     /locus_tag="VFQ54_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164972.1"
                     /translation="MPNRRHIVLSLTVLCSLIFSSLITILASTTAAAQSNATPVAATP
                     FITADGIDSHVLAQSDPAPITIPNLHLVLERLEIPASVALPERTTTGPEILYVENGSV
                     SIEDSVGFATSTAAGEELTFNPGASYALTNDGFLAATVLRLSFGGTTAAATSPVPSAA
                     TPVTAARITTLIDQPVTDLPTGDATLFVADATFAAGAES"
     gene            complement(801..2039)
                     /locus_tag="VFQ54_08005"
     CDS             complement(801..2039)
                     /locus_tag="VFQ54_08005"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164973.1"
                     /translation="MFALLAANLISQIGNTFSYLAIPWFVLATTGSASQTGITVAVGT
                     IPLVVVGIFGGTIVDRLGYRRSSIISDLFSGFSVLLIPLLHATVGLAFWQLLVLVFLG
                     AVLDGPGSSARSALYPELVERAGIPLERANGIYYAAGRAAELLGPPLAGILIAAIGPS
                     DLLWINAVSFAISAGIVATVIPTIVNSDAAQQAFRGLRGYVVQVREGFQFLFGNQMLL
                     WLLLSFSIGSLIAEPVYQVVLPVYAKDVLKSSVQLGFIFAAMGAGSLIGNLIFTTIGL
                     RLSRRMILLGGIGIRAATFIVLLTTPPWWVIAAAIFIGSVALEPINPMSMSIMQEQVP
                     PGMRGRVFGARIAIQGGTLPLGILLYGFLIAGIGLHPTLWTLVIANLALPVFMALLPS
                     LRDMRRPEPAPTPTPVATAD"
     gene            2542..>2909
                     /locus_tag="VFQ54_08010"
     CDS             2542..>2909
                     /locus_tag="VFQ54_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0164974.1"
                     /translation="MNWSASLVNQSSSDQPTRRPAGRPRSFDDADVFAATNRALVREG
                     YANLTLAAIARELGITGPALNRRYGDKHTLMEAYIGWLTDQMVVRLADAKAHYPSSPL
                     NVLRARWYIPVPGDSALEENR"
BASE COUNT          572 a          826 c         1019 g          492 t
ORIGIN      
        1 cgctctccgc gcccgcggcg aaggtcgcat cggctacgaa gagcgtcgca tccccggtcg
       61 ggagatcggt gaccggctgg tcgatcagtg tcgtgattct ggcggcggtg accggcgtcg
      121 ccgcggacgg aaccggcgaa gtggccgcgg cagtcgtgcc gccgaaactg agacggagaa
      181 cggtcgcggc gaggaagccg tcattggtca gggcatagct cgcgcccgga ttgaaggtca
      241 gttcctcacc ggcggcagta gaggtcgcaa agccgacgct gtcctcgatc gaaacactgc
      301 cattctcgac atagagaatc tcgggtccgg tcgtcgtgcg ctcggggagg gcgacacttg
      361 ccggaatctc gagacgctcg aggacaaggt ggagattcgg gatcgtgatc ggggcggggt
      421 cggactgcgc gagtacgtgg gaatcgatgc cgtcggcggt gatgaacggc gtggcggcga
      481 ccggggtcgc gttcgactgg gcggcggcgg tggttgatgc aaggatcgtg atgagggagg
      541 agaagatcag actgcagaga acagtcaggg aaaggacgat atgacgtcgg ttcggcatgg
      601 cgcactgctc ctgagtcgat ttaattgcat taagggtgtc ccaccaggag tgtagtgggc
      661 ggagttggga cacgcaagag gagtcgaggc ggatttcttg cggataacga caacttaatg
      721 aaggcggcat acggagaccc ggaggcttca tcggcctccg ggtctctggc gtgatacagg
      781 gcgtctcgcc cggagcgcgc tcagtcggca gtggcgaccg gggtgggcgt tggcgccggt
      841 tccgggcggc gcatgtcgcg gagggagggg agtagcgcca tgaagaccgg cagcgcgagg
      901 ttcgcgatca ccagcgtcca gagcgtcggg tggagaccga tcccggcgat caggaagccg
      961 tagaggagga tcccgagcgg caaggtcccg ccctggatcg cgatccgcgc cccgaagacg
     1021 cgaccgcgca tccccggtgg cacctgctcc tgcatgatcg acatcgacat cgggttgatc
     1081 ggctcgagtg cgaccgagcc gatgaagatc gccgccgcga tcacccacca cggcggggtc
     1141 gtcagcagca cgatgaaggt cgcggcgcgg atgccaatcc ccccgagcag gatcatccgc
     1201 cgcgagaggc gtaggccgat cgtggtgaag atcaggttgc cgatcagcga gccggcgccc
     1261 atcgccgcga agatgaagcc cagctggacc gaactcttca ggacatcttt cgcatagacc
     1321 gggaggacca cctggtagac cggttccgcg atcaggctgc cgatcgaaaa ggagaggagg
     1381 agccagagca gcatctggtt gccgaagagg aactggaatc cctcccggac ctgtacgaca
     1441 tatccacgca gcccccggaa tgcctgctgg gcggcatcgc tgttgacgat cgtcgggatg
     1501 acggtggcga cgatacccgc cgagattgcg aaggagacgg cgttgatcca gagcagatcg
     1561 ctgggaccga tcgcggcgat caggatcccg gcgagcggtg gtcccagcag ctcggcggcg
     1621 cgtcctgccg cgtagtagat gccgttcgcg cgttcgagcg ggatgcctgc gcgttcgacg
     1681 agttccggat agagcgcgga gcgggcgctg cttcccgggc cgtcgagcac ggcgccgagg
     1741 aagacgagca cgagcaactg ccagaaggca agcccgaccg tcgcgtggag gagcgggatg
     1801 agcaggacgg agaacccgct gaagagatcg gagatgatgc tcgatcgcct gtagccaagg
     1861 cggtcgacaa tcgtgccgcc gaagatgccg acgaccacga gcgggatcgt gccgacggcg
     1921 acggtgatcc cggtctggct ggcgctgccg gtggtggcga ggacaaacca ggggatcgcc
     1981 aggtagctga aggtgttgcc gatttgcgag atcaggtttg cggcgaggag cgcgaacatc
     2041 ggcacggtgt tgctccggcc gatggagcct ggggaggggg tatcggacga tctatcgctt
     2101 tccatatacg acgtgtcctc ccgaacaaag actcgccgcc gctcgctgcg tggatcatag
     2161 gtgaagtcat gccgatgtgt cgcatcggga ggaactgtct ctacgaaatc atgcattggc
     2221 accggaggcg tctcacagct aatccacatg ttattcatcg cgcattccaa gtccaatatc
     2281 cgaccgttac ggacgtcagt ttgtggtgaa gaaacaggtg aacctcatca cattgacgac
     2341 attcagcgtt tttcgggtgc gatgggtttg ctgaacggtg ggaaaacagt cgatgcggat
     2401 acgtcattgg ggttatactt atccaatacg ggcaatcgca ttgcgatccg agacgtgtca
     2461 gaggttgtgg agacgggacc tttgttgcga gcgaccccct tttggaatcg ctcatcgcgc
     2521 ccatcgtcgc aaggatggac catgaactgg agcgcttcgc tggtcaacca atcatcttcc
     2581 gatcaaccca cccggcgccc cgccggtcgg cctcgttcat tcgatgacgc cgatgttttc
     2641 gcggcaacga accgggcgct ggtccgtgag gggtacgcga acctcaccct ggcggcgatc
     2701 gcccgggaac tcgggatcac cggaccggct ttgaaccgcc gctacggcga caagcacacc
     2761 ctgatggaag cctacatcgg ctggctgacg gatcagatgg tggtccgcct cgcggatgcg
     2821 aaggcgcact acccatcctc gccgctcaac gtgctgcgcg cgcgctggta catcccggtg
     2881 ccgggtgatt ccgcgctgga agagaatcg
//
LOCUS       DASTMD010000549         7135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_408995, whole genome shotgun sequence.
ACCESSION   DASTMD010000549 DASTMD010000000
VERSION     DASTMD010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408995"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1066
                     /locus_tag="VFQ54_08015"
     CDS             32..1066
                     /locus_tag="VFQ54_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0164975.1"
                     /translation="MHERMAIATTRKPFQIGFFSYLEGHELPSEIYRKAVETYIRADE
                     LGIDKAWVAHHHFGHHGGLPTPLVFFASLAGRTKHLGFGTAVLTLPTENAIHLAEDAA
                     VFETLHPGRLDLGFGTGFGSDAVLDTFGQSNKNRREVYNEAILRLRDAFNGEILNSAG
                     DTLYPEAPQLNRRLWESPGSVPNVIAAARRGNGLLLSRVAIGTDRDTDEVQRELVDAY
                     YSALPKGVEPRIALSRTVYPTNDPESAYQNLARGVQAGRDSAARQGRPQPERTMEEDF
                     EHFSIHWGEPDQVIATLRQEPLLGEITDLVCQLSPGTQTLEQNLEALELLATKVGPAL
                     GWTPANAVTV"
     gene            complement(1337..2356)
                     /locus_tag="VFQ54_08020"
     CDS             complement(1337..2356)
                     /locus_tag="VFQ54_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0164976.1"
                     /translation="MSRLVTMNRRPAGWSDVELRHLVAALSRRRLLAGSAASGLFAML
                     AGSETFAQEATPAAGMRIVQTANGPVQVPVNPLRVVCADYYSPFAVFDAGIVPRGAID
                     GLASNLLPEYLDGYASLRKIGAFGEPDLEAIVDLRPDLIVGSADYSGAYDRLRAIAPT
                     TLFDFGDSGDWPAFSESIADAVGRRDEFATINHIYRERAGVINKGYAATLSSTRWAIV
                     NGAGDQWWLYNPDSSHGLVLSAAGVRFIDEVEGKAKDFVELPYEKIDLLAGADVIIVR
                     ANSDGTVNEETRALLDQPLFKSLKAAQAGRVFPLSALFPFSYEMAIRLLDQIEVVASI
                     LQTKS"
     gene            2595..3068
                     /locus_tag="VFQ54_08025"
     CDS             2595..3068
                     /locus_tag="VFQ54_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0164977.1"
                     /translation="MTRPSNFQPAYGQLLNISQYLASTSIDQHLLDLISLRASQINGC
                     AFCLTMHVRDLLGRGERQDRIAVLPAWRETTWFTDRERAALAWTETLTILGEHGVSDE
                     LFVTASEQFSERELADLTLAVILINGWNRLNIAFQNPPAPFTDDEIAIAATTVSV"
     gene            3080..4015
                     /gene="sigJ"
                     /locus_tag="VFQ54_08030"
     CDS             3080..4015
                     /gene="sigJ"
                     /locus_tag="VFQ54_08030"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HEU0164978.1"
                     /translation="MIARSTMVEQISSEIYDGLRRYLFAVAYRMTGSAGDAEDLVQDS
                     WIRYLDAGSPAVASLRAYLTTVVSRLSLDYLKSARIQRESYVGEWLPEPVLTSEAIPG
                     PAEMVEQREEISIAFLTLLEQLSPEQRAVYVLREGFGLPFEEIGVHTGRTAAACRQLY
                     RRAQRALPGHPLPATSRVDTHRELIERFVTALEQGSVEGVTKLLSEHVLWVGDGGPNR
                     LANRRPIVGRDRVTRGLLAYQAKPSLPTAVWTIEHLNGFPAVVYYVDGTVDRVISFDI
                     LGEEIVAIRGILNRDKLAHLQRSLDDIGTMRGNTP"
     gene            4171..4629
                     /locus_tag="VFQ54_08035"
     CDS             4171..4629
                     /locus_tag="VFQ54_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164979.1"
                     /translation="MDLALVAVIIGVIAATAGVVLIVGVVMIGRSAAQAQQAASAAPE
                     PVEISDDDAHASEFDPLGSDEDDPNAFVDPFAKETAEFDPVESETDDPNAFVDPFAEQ
                     DDEDKDDALEWGSPFDEPAEEPAVKWGFDGDDEPAADETAPSEKRERGSE"
     gene            4626..4823
                     /locus_tag="VFQ54_08040"
     CDS             4626..4823
                     /locus_tag="VFQ54_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164980.1"
                     /translation="MNVLGIGPPEVGIVLLISVFTLAPIVGFIMLFRRLGNILRETRA
                     QGARLRTLQETIHPDVRRDDV"
     gene            4820..5101
                     /locus_tag="VFQ54_08045"
     CDS             4820..5101
                     /locus_tag="VFQ54_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164981.1"
                     /translation="MTLPPLETTTGDLKGYFLLIFGLAFCGIAIVFLFFAVSIVRSVR
                     RSRQESADGIAVEQLATITAQQQATSDSGAGPEPSKADDGQSTDPIERP"
     gene            5259..5474
                     /locus_tag="VFQ54_08050"
     CDS             5259..5474
                     /locus_tag="VFQ54_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164982.1"
                     /translation="MTIVLELAIVAIALVSGVFAIIRSMSGRQHPEGGSAGLISGRRH
                     PRPDPDANPVYEWNDADWNEQRDRRAS"
     gene            complement(5538..6821)
                     /locus_tag="VFQ54_08055"
     CDS             complement(5538..6821)
                     /locus_tag="VFQ54_08055"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0164983.1"
                     /translation="MRPGLGTPRIQMGAFLIGNGVSLIGNTLTLVGLPWFVLQTTGSA
                     GKTGLTGMAFALPAFLAGLFGGILIDRLGGRRMSAIADVVSGTSVALIPLLHLTVGLS
                     FWQLLILVFFGAMLDIPGLTARRTMLPQLSERAGIRPEAMNSAFETTDAFAIIVGPAI
                     AGLLIAWIGAVNLLLFDAATFAISALLIGLVVPDTIAATARDDDMVEGVGSAWSEILA
                     GLHFIRADRLLLALALILVFTNFFNGPFFTVIMPVIVNDRYGAASEYGLLLTAFGIGS
                     LMGGAAYGAFGHRLRAHRLELWLVGFASVAVIRWLLVPDYPYVLLLATMALAGLLSGP
                     VNPLLVTVRFERIPVHLRGRVFATFAALASIADPLGMATVGYAIEQVGLHETLVVTAV
                     LFTALVLIVPFIRVIRQMNLPAAEGDPETVTPPRE"
BASE COUNT         1410 a         2404 c         2084 g         1237 t
ORIGIN      
        1 cagaattcct ctcgcgccct catgaaatcg gatgcatgaa cgtatggcga tcgcgacaac
       61 caggaaacca ttccagatcg gattcttctc ctacctcgaa ggtcacgagc ttccgtccga
      121 gatctaccgc aaggcagtgg agacctatat tcgcgccgac gagcttggga tcgacaaggc
      181 atgggtcgcc catcatcact tcgggcacca tggcgggctg ccgacgccgc tcgtcttctt
      241 cgcatcgctg gccggtcgca cgaagcacct cggcttcggg acggcggtgc tgacgctccc
      301 gaccgagaat gccatccacc ttgccgagga tgccgcggtc ttcgagaccc tgcacccggg
      361 tcggctcgat ctcggcttcg gcaccggttt cggctcggat gccgtgctcg acaccttcgg
      421 gcagtcgaac aagaaccgtc gcgaggtcta caacgaggca atcctccgcc tccgcgatgc
      481 cttcaatggc gagatcctca acagcgccgg cgataccctc tacccggaag cgccccagct
      541 caatcgtcgc ctctgggagt cgccgggctc ggtgccgaat gtgatcgcgg cggccaggcg
      601 cggcaacgga ctcctgctct ctcgcgtcgc gatcggcacc gatcgcgaca ccgatgaggt
      661 gcagcgcgaa ctggtcgacg cctactattc ggcgctgccg aagggcgtcg agccgcgcat
      721 cgccctctcg cgcacggtct acccgacgaa cgacccggag agcgcctacc agaacctcgc
      781 ccgcggcgta caggcggggc gcgattccgc ggcgcgccag ggccggccgc agcctgagcg
      841 gacgatggag gaagacttcg agcacttcag catccactgg ggtgagccgg accaggtgat
      901 cgcgacactc cggcaggagc ctcttctcgg cgagatcacc gatctcgtct gccaactctc
      961 accgggcacg cagacactgg agcagaatct cgaagcgctg gaactgctcg ccacgaaggt
     1021 tggtccggcc ctgggctgga ctcctgccaa cgccgtcacc gtctagcccc gtgtcgcctt
     1081 cgctgcacgc cttcgagaaa gacgccgcat cggttcatcg tggatccatc cataggcgtc
     1141 tgcccacggg gcgtgctctc ggcctccttg ccgccgggag cacgccccgt ggcatcgcca
     1201 cggcagccgg ttagcgggtt gatgcaatat ggtcttcaat cgcatcatgc tgccggtcac
     1261 ggaccggtgc ttcatgtcac agagggtcgc cagtctccct ccaccaggag tcacgcccct
     1321 atctcgtctg tgagggctag ctcttggttt gcaggatcga cgcaactacc tcgatctgat
     1381 cgagcaaccg gattgccatc tcgtagctga acgggaagag tgccgagagt ggaaagaccc
     1441 gacccgcctg tgccgccttc agcgacttga agagaggttg atccagcagc gcccgggttt
     1501 cctcgttaac cgtaccgtca ctattcgcac ggacgatgat cacgtctgct cccgcgagca
     1561 gatcgatctt ctcgtacggg agttccacga agtcctttgc cttgccttcg acttcgtcga
     1621 taaaccggac gcccgcggcg gacaggacca aaccgtgtga cgagtcgggg ttgtacagcc
     1681 accattgatc gccggcgccg ttgacgatcg cccaacgcgt agacgagagg gtggccgcgt
     1741 atcctttgtt gatgacgccg gcacgctcgc ggtatatgtg attgatggtc gcgaactcgt
     1801 ccctccgccc gaccgcatcg gcgatcgact cggaaaacgc aggccaatca cccgaatcac
     1861 cgaaatcgaa gagcgtcgtc ggtgcgatcg ctctcaaccg atcgtaggcg ccagaataat
     1921 ccgcggatcc cacaatcaag tcgggacgca gatcaacgat cgcctcgaga tcgggttcac
     1981 caaatgcccc gatcttccgg agtgaagcat atccatccag atactccgga aggagattac
     2041 ttgccagtcc gtcgatcgcc ccgcgtggca cgatgcctgc atcgaagacg gcgaagggcg
     2101 aatagtagtc ggcgcagacc acgcgaaggg gattgacggg aacctgaacc ggtccgttgg
     2161 ccgtctggac aatgcgcatc cctgccgccg gagtggcttc ctgcgcgaac gtttcggaac
     2221 cggcaagcat cgcaaacagg ccgctcgcgg ctgatccggc aagaagcctg cggcggctca
     2281 gtgccgcaac cagatgtcgg agttcgacat cactccatcc cgcaggacgc cgattcatcg
     2341 tgacaaggcg agacatccag attcctcctg acgacactcc acaggatgaa agccgactaa
     2401 agtagtcgga tttcatccta cggggagctc gatcgatgcg tcaagagacc tccatccgca
     2461 tgcgatccga atgcggatgg aatcgatgcg cagcgcggcg tcacattccg cgccgctgaa
     2521 tcgtcttctg gtcagcagcg acgaagctgc gatcgatctt ccgcacacag gagtttccga
     2581 cgatgaccgg acgcatgacc cgcccatcga acttccagcc cgcatacggg cagctgctca
     2641 acatctcgca gtacctcgcc tcgacatcga tcgatcaaca tctgctcgat ctcatctcgc
     2701 ttcgcgcctc ccagatcaac ggctgcgcct tctgcctcac gatgcacgtc cgcgatctcc
     2761 tcggccgcgg cgagcgccag gaccgcatcg ccgtcctccc tgcctggcgg gagaccacat
     2821 ggtttaccga ccgcgagcgc gccgccctcg cctggaccga gacgctgacc atcctcggcg
     2881 agcacggcgt ctccgacgag ctcttcgtca cggcgagcga gcagttctcg gaaagggaac
     2941 tcgcggacct tacacttgcc gtcattctta tcaacggctg gaaccgtctc aacatcgcct
     3001 tccagaaccc gccggcgccg ttcaccgacg acgagatcgc catcgccgcc acgaccgttt
     3061 ccgtctaggg atttcgtcga tgatcgccag gagcacgatg gtcgagcaga tcagcagcga
     3121 gatatacgac ggccttcgcc ggtatctctt cgccgtcgca taccggatga ccggcagcgc
     3181 cggcgatgcg gaggacctcg tccaggattc ctggatccgc tatctcgacg cgggatcacc
     3241 cgccgttgcc tccctgcgcg cctatctcac gaccgtcgtt tcccggctct cgctcgacta
     3301 tctgaaatcc gcccgcatcc agcgcgaatc gtatgtcggg gaatggctac cggaaccggt
     3361 gctgacgtcc gaagccatac ctggtccggc ggagatggtc gagcagcgcg aagaaatctc
     3421 gatcgccttc ctcacgttgc tcgaacaact ctccccggaa cagcgcgccg tctacgtcct
     3481 tcgcgaaggc ttcggtcttc ctttcgagga gatcggcgtc cacacgggtc ggacggccgc
     3541 cgcctgtcgc cagctctacc ggcgcgccca gagagccctc ccggggcacc ctctccctgc
     3601 gacctcccgg gtcgacaccc accgcgagct gatcgaacgc ttcgtcaccg cgctcgaaca
     3661 gggcagcgtc gaaggcgtga ccaagctcct ctccgagcac gtcctctggg tcggcgacgg
     3721 cggcccgaac cggctcgcca accggcgtcc catcgtcgga cgcgatcgcg tcacccgcgg
     3781 gttgctcgca taccaggcga agccgagtct tcccaccgcg gtatggacca tcgagcacct
     3841 caacggtttc cccgcggtcg tctactacgt cgacggcact gtcgatcgcg tcatttcctt
     3901 cgacatcctc ggcgaggaga tcgtcgccat ccgtggcatt ctcaatcgcg acaagctggc
     3961 gcacctccag cgctccctcg acgacatcgg gacaatgcgc ggcaacacgc cctgaccaca
     4021 gctcattccg gcaagaagaa acaatcgtcc atatccggcg accccgcgat cacgggcgca
     4081 catggcgatc accgccgatc gaagacacga cctgggctag acttggggca catcgatcgt
     4141 cgtcattcgt accgtagctg gagatctggg atggacctcg cactcgtcgc cgtcatcatc
     4201 ggcgtgatcg ccgccaccgc aggcgtcgtc ctcatcgtcg gcgtagtcat gattggacgc
     4261 agtgccgctc aggcccagca ggccgcgtcc gcggctccag agccggtcga gatctcggat
     4321 gacgacgcgc acgcctcgga gttcgatccg cttggcagcg acgaggacga tcccaacgcc
     4381 ttcgtcgacc ccttcgccaa ggaaaccgcg gaattcgatc cggtcgaatc cgaaaccgac
     4441 gaccccaatg ccttcgtcga tccgttcgcc gagcaggacg acgaagacaa ggacgacgcc
     4501 ctcgaatggg gatcaccgtt cgacgagcca gccgaggagc cagccgtcaa atggggtttc
     4561 gacggcgatg acgagccggc agcggatgaa accgcacctt cggagaagcg ggagcggggt
     4621 tccgaatgaa cgtgctgggg atcgggccgc cagaagtagg catcgttctg ctcatatcgg
     4681 tgttcacgct cgcacccatc gtcggcttca tcatgctctt ccggcggctt ggcaatatcc
     4741 tgcgcgagac gcgcgcccag ggcgcccggc tccgaaccct ccaggagacc attcatcccg
     4801 atgtccgacg ggatgacgtg tgacgctacc cccgctcgaa acaacgaccg gcgacctgaa
     4861 ggggtacttt ctgctgatct tcggcctcgc gttctgcggc attgccatcg tcttcctctt
     4921 cttcgcggtc tcgatcgtcc gctccgtccg gcgctcgcgt caggaatctg ccgacggcat
     4981 tgccgtggag cagctggcga cgatcaccgc gcagcagcaa gccaccagtg attccggggc
     5041 cgggccagaa ccatcgaaag cagacgatgg gcaatcgaca gacccgatcg aacgcccgtg
     5101 atcattgaca ccaattaatc tttggcgcat aaattgcgta acttgaaaca cttgctgctg
     5161 cgttcccgcg cgcacccgcc ggcaggttcc gggatcgctc cacgatcccg tcgaactggt
     5221 cgcttgccac ccacctctgt ccgcaggggg attccgccat gaccatcgtt ctcgaactcg
     5281 ccatcgtggc aatagccctg gtttccggtg tcttcgccat catccggtcg atgtcgggac
     5341 gccagcatcc agagggcggc agcgccggtc tgatttcggg acgccgccat ccacgtcccg
     5401 acccggatgc caatcccgtc tatgagtgga atgacgcgga ctggaacgaa cagcgggatc
     5461 gccgcgcttc ctgagacacg gtttcatgcc gatcttcggg gcaccgggcc gcccacgcga
     5521 cccggctttc gtgcgtccta ttccctgggc ggtgtcaccg tttcgggatc gccttcggcg
     5581 gcggggagat tcatctgccg aatgacacga atgaacggca cgatgagcac aagcgccgtg
     5641 aagagcaccg ccgttaccac gagcgtctcg tgcagaccca cctgctcgat cgcatatccg
     5701 acggtcgcca tgccgagggg atcggcgatg gacgcgagcg cggcgaacgt ggcgaagacc
     5761 cttccccgga gatgcaccgg aatccgctcg aagcgaaccg taaccagcag cgggttcacc
     5821 ggtccgctca ggagtccggc aagcgccatc gtcgccaaga gcagaacgta agggtaatcc
     5881 ggcaccagta gccaccggat gacggcgacc gacgcgaagc caacgagcca cagttccagc
     5941 cgatgagccc ggaggcgatg tccgaacgcg ccgtaggccg caccacccat caggctgccg
     6001 atcccgaacg cggtcaacaa caggccatat tccgaagccg cgccataccg gtcgttaacg
     6061 atcaccggca tgatcaccgt gaagaatggt ccgttgaaga agttcgtaaa gacgaggatc
     6121 aacgccagcg ccagcagcag gcgatcggcg cgaatgaagt gcaacccggc caggatctcc
     6181 gaccaggctg accctacccc ttccaccatg tcgtcgtccc gcgccgtagc ggcgatggtg
     6241 tccggaacaa ccaggccgat cagaagcgcc gagatcgcaa aagtcgcggc gtcgaagagg
     6301 agaagattga cggcgcctat ccaggcgatg agaaggccgg caatcgcagg tcccacgatg
     6361 atcgcaaatg cgtcggttgt ctcgaacgcg gaattcatcg cttccggccg gatgccggcg
     6421 cgctcggaaa gctggggcag catcgttcgc cgcgcggtca ggcccggaat atcgagcatc
     6481 gccccgaaga agacgagaat cagaagctgc cagaacgaga gtccaaccgt cagatgcagc
     6541 agtgggatga gcgcaacgga ggttccgctg acgacatcgg cgatcgcgct catccggcgt
     6601 ccgccgaggc gatcgatcag gatgccgccg aacaggccgg cgaggaacgc cggcaaggcg
     6661 aacgccatgc cggtcagccc tgtcttgccg gcgcttccgg tcgtctggag aacgaaccat
     6721 ggcaggccaa cgagcgttag cgtgttgccg atcagcgaga caccgttccc gatcaggaac
     6781 gcccccatct gaatgcgcgg agtgccgagg cccggcctca tatccggttg tcctcaacat
     6841 cgaggttccg atggaacacg cacgatccct gacccacgtc ggaatcccgg gaactcccgg
     6901 ctccggacgg aattgaaacc cgtgttgacg ccgccagacg acgtgtcctg cgttcaactg
     6961 aattgatgaa gcccatgtga tcgaggaatc tcgcctgcag gcagacaatc cgcgccaaag
     7021 acgtgtaaca ggaaagatag cggatcggcc catcctattc acgcatttct tgcgttgcgt
     7081 ctattttgcc tatatacaaa tcggatgaat ccacaatctg ccttcggttg aacgc
//
LOCUS       DASTMD010000550         3249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2730278, whole genome shotgun sequence.
ACCESSION   DASTMD010000550 DASTMD010000000
VERSION     DASTMD010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2730278"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFQ54_08060"
     CDS             complement(<1..183)
                     /locus_tag="VFQ54_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164984.1"
                     /translation="MSQDGRDEVKPGAGARPRRGGLGRGLESLIPTTPSPATTRSTGD
                     EVATTVSGEPLIVPLGS"
     gene            422..1351
                     /locus_tag="VFQ54_08065"
     CDS             422..1351
                     /locus_tag="VFQ54_08065"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEU0164985.1"
                     /translation="MTDTISGAFLLLFFVGLLGGLVYWAKRAETDRSAYVGLYLVFGI
                     PGLLLTVAGLALSVNRVDAGPLALGCGLALGLPMIKRVRVQIARVTPMDPDSPIDMAG
                     LATILLVIMISLLTLNDTDTSSSSDDVTIATLLINVIAFVSIAYVCVGYRIWRTGEDA
                     TQRLGIRVPSPRTVLIALGCVIPAFISSIIGSALTQAFQPDVIDNLEHTVSDMTSGVN
                     NPFGALLLGLSTGVGEEVLFRGAIQPRYGMVLASICWALLHAQYGFTFVLVGLFGIGI
                     LLGLERKYLGTSAAIITHATYNTILVLLQTYTN"
     gene            1432..3096
                     /locus_tag="VFQ54_08070"
     CDS             1432..3096
                     /locus_tag="VFQ54_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU0164986.1"
                     /translation="MEIVEIRDLDGPNLFLLRPAIKLEVAFGAAEEPLVSRIAEAFVA
                     GEVTESRELVPGGIERVLVLLAESINVLHDRLGVERPEMISRWMETPGHYVVAYSWER
                     RKLGRDLAHIAFELVAGKLSNADLDVRLAAMQHHLVTPAKPGDEPEYLRDTARHLPII
                     GITGTNGKTTTTRLVSAVLMNAGHRVGWTSTAGVFVQGEERVKGDFTGPGGAAHVLKE
                     PDLEFAVLETARGGILLRGIGYESNDVSVVTNISPDHLGLHGVQTVEGLAEVKAVVAR
                     VTRPEGFVVLNADDPLVLAMRESIVARPFLISRDDENPAVREHVAAGGWALTVQSGQI
                     AWFHEGLREIVTDLADVPVTFGGRAAHMVENALCGTAACIAIGLPIDQVRAGLAAFQP
                     SAEHNRGRLNVFDVQERTYIIDYAHNEAGLVHLIALGRSFASERNARLIAIIGSAGDR
                     TDNALRILGSTSARSADLTIIKETNHYLRGREKDEITRFIKEGMTSEGVEWAAIASSE
                     FDGVQRAFELSSPGDVIVVMSTEDYDDILAWFEEMAEERIAPETGS"
BASE COUNT          536 a         1041 c         1064 g          608 t
ORIGIN      
        1 ggaaccgagc ggaacgatca ggggttcgcc agagacggtg gttgccacct catcgccggt
       61 cgatcgggtc gtggccggtg acggggtcgt cgggatcagc gattcgagcc cgcgaccgag
      121 cccgccacgg cgtggccgtg cacccgctcc cggcttcact tcatcccgtc cgtcctgact
      181 caccgcagcg ctccgtctct tgccgtgttg cggccatgca cgatcgtggg aatcgatccc
      241 cgactctgcg aacgccgcgc tcggaatcac gcgttcagtc tagcatcgtg gcccggaacc
      301 tcgggcgccg cgccacgtat acttcgggct gcatgatccg gaagacattt cacccgccgg
      361 agaccccggt gggtccgatc gcacgacggc gccgcagccg cgaggagcac gatccggccc
      421 catgaccgat acgatctcgg gagctttcct ccttctcttc ttcgtcgggt tgctcggtgg
      481 gctggtctac tgggcaaaac gggccgaaac cgatcgctcc gcctatgtcg gactctatct
      541 cgtcttcggg atccccggat tgctgctgac cgtggccggt ctcgcccttt ccgtgaaccg
      601 cgtcgacgcc gggcccctgg cgctcgggtg tggcctggcg ctgggactgc cgatgatcaa
      661 gcgggttcgc gtccagatcg cccgggtgac cccgatggat ccggacagcc cgatcgacat
      721 ggccgggctc gcgacgatcc tgctcgtgat catgatctcg ctgctcaccc tcaacgacac
      781 ggatacgtcg tcttcctcgg acgacgtgac gatcgcgacg ctgctcatca acgtgatcgc
      841 gttcgtctcg atcgcctatg tctgcgtcgg ctaccggatc tggcggaccg gtgaagacgc
      901 aacccaacga ctcggcatcc gcgtgccttc gccgcgaacc gtcctgatcg cccttggctg
      961 cgtgatcccg gccttcatct ccagcatcat cggttccgcg ctgacgcagg cattccagcc
     1021 ggacgtgatc gacaatctcg agcacaccgt cagtgacatg acgagcggcg tgaacaaccc
     1081 gttcggtgcg ctccttctcg gactctcgac gggtgtcggt gaggaggtgc tgttccgcgg
     1141 cgcgatccag ccgcgatacg gcatggtgct cgcctcgatc tgctgggctt tgttgcacgc
     1201 gcagtatgga ttcacctttg ttttggtggg attgttcggg atcgggatcc tgctcgggct
     1261 ggagcggaag tatctcggaa cgtcggcggc gatcatcacg cacgcgactt acaatacgat
     1321 cctggtgctc ctccagacgt acaccaactg acgtcctgta cctgggccca ttgcacgcag
     1381 aatcgagttg gacagggcgt cgccctcccc ttaatccgag tgaggacaag catggagatc
     1441 gtcgagatcc gcgacctgga tggtccgaac ctctttctgt tgcggcccgc gatcaagctc
     1501 gaggtggcgt tcggcgcggc ggaggagccg cttgtcagcc gcatcgcgga ggcgttcgtc
     1561 gccggcgagg tcacggagag ccgggagctg gtcccaggcg ggatcgagcg cgtcctcgtg
     1621 ctgctggccg agtcgatcaa cgtcctgcac gaccggctcg gggtcgagcg gcccgagatg
     1681 atctcccgct ggatggagac acccggtcat tacgtcgttg cctatagctg ggagcggcgg
     1741 aaactcggtc gcgatctcgc tcacatcgcg ttcgagctgg tggcggggaa gctctccaac
     1801 gccgatctcg acgtccggct tgcggcgatg cagcatcatc tcgtgactcc cgcgaagccc
     1861 ggagacgagc ccgagtatct tcgcgacacc gcccgccatc tcccgatcat cggcatcacc
     1921 gggacgaacg ggaagacgac gacgacgcga cttgtctcgg cggtcctgat gaatgccggc
     1981 caccgcgtcg gctggacctc gacggccggc gtcttcgtcc agggtgagga acgcgtgaag
     2041 ggggacttca cgggaccggg cggtgcggcc cacgtcctga aggagcccga tctcgagttc
     2101 gccgttctcg aaaccgcgcg cggtggcatc ctcctgcggg ggattggcta cgagagcaat
     2161 gacgtcagcg tcgtcacgaa catctccccc gatcacctgg gactgcatgg cgtgcagacg
     2221 gtggaggggc tcgccgaagt gaaggccgtc gtcgcgcggg tgacgaggcc ggagggattc
     2281 gtcgtgctca atgcggacga ccccctcgtt ctcgcgatgc gggagagcat cgtcgcccga
     2341 cctttcctga tctcgcgcga tgacgagaac ccggctgttc gcgagcacgt cgcggcgggt
     2401 ggatgggcat tgacggtgca gagtggccag atcgcctggt tccatgaggg gctgcgcgag
     2461 atcgtgaccg atctcgccga tgttccggtc acgttcggcg ggcgtgccgc gcacatggtc
     2521 gagaacgcgc tctgcgggac ggcggcctgc atcgcgatcg ggctgccgat cgatcaggtg
     2581 cgggcggggc tcgcggcgtt ccagccgagc gccgagcaca accgggggcg cctcaacgtc
     2641 ttcgacgtcc aggagcgcac gtacatcatc gattacgcac acaacgaggc cggcctcgtt
     2701 cacctgatcg cgctcggccg ctccttcgcg agcgagcgca acgcccggct gatcgcgatc
     2761 atcggttccg ccggcgatcg cacggacaat gcgctccgga ttctcggctc gacgtcggcg
     2821 cggagcgcgg atctcacgat catcaaggag acgaatcact atctccgggg tcgcgagaag
     2881 gacgagatca cccgcttcat caaggagggc atgacgtccg aaggagtgga gtgggccgcg
     2941 atcgcctcgt cggagttcga cggcgttcag cgcgccttcg aactttccag tccgggtgat
     3001 gtgattgtcg tgatgagcac ggaggattac gacgacatcc tcgcgtggtt cgaggagatg
     3061 gccgaggaac gtatcgcgcc ggaaacgggt tcgtgatctc gcgcccgaga atgattctcg
     3121 aaaagtcggc tagtttacct ttgacgccat cgcttacggt tggtacgctt ccgcgtagac
     3181 gtcacaacga gccagacgaa cgagtcaggt cggtggcctc ttgtcatcca cggagattgc
     3241 agcacatgg
//
LOCUS       DASTMD010000551         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1034787, whole genome shotgun sequence.
ACCESSION   DASTMD010000551 DASTMD010000000
VERSION     DASTMD010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034787"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VFQ54_08075"
     CDS             <1..672
                     /locus_tag="VFQ54_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0164987.1"
                     /translation="QMARYVIFLYQAGGNLTNAEVTEIELNSDAAKTALAEMHGLYSD
                     GLSVASADIGAGWPGDALAQGLTSIAFEGNWLFPFLVTNAPDLKYAIAELPAGPAGKG
                     TPAFTNSYSIFKGSKNPDAAWVLVNFLTGPEGAEIAGELGLAIPGRPDLEAGWLKLFP
                     DRQPYIAAGKYATAVQYGPGGQKFQTDADAILQSLWAGQIEESAAIQQIYDTAKSDIT
                     LTGGQ"
     gene            777..1760
                     /locus_tag="VFQ54_08080"
     CDS             777..1760
                     /locus_tag="VFQ54_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0164988.1"
                     /translation="MIAIPRRWKPRNPTAAEDAIVGWLFIAPALVVISVFVFWPILQI
                     FWLSFHQWSVITPEKPWVGLRNYRDFFNNPDFETALRNTLWYSIGVVPIQTFIAVFLA
                     VVANQKIRGRGFFRTAFYFPSISSTVVIAVIFLWLYQQNGLINFTLRKIGFSPPRPPW
                     LANPKGVIEMVLNGVGVHHVDPWFAGPSVALLSIMMMAIWTSVGGYMVIFLAGLQDIP
                     AEVYEAAALDGASKRQQFTQITLPLLRPVIFFVVTLGFIGTFQVFDSIYIMAPGGSPA
                     KTTLTLGYLVYQQGFQNFAMGYASAIAIVLFAIILGIYLFQRRFLGRTDQR"
     gene            1773..>2087
                     /locus_tag="VFQ54_08085"
     CDS             1773..>2087
                     /locus_tag="VFQ54_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164989.1"
                     /translation="MAVITSETIKAGQGGEVRGRRSIGQTIVVTIAYVILVAVALLMF
                     VPFLFSLATSFKTPPDALRLTFSSMFWPRTWTLDAYRTALDSNIQRWFINSAVVAAIW
                     ICG"
BASE COUNT          362 a          720 c          586 g          419 t
ORIGIN      
        1 cagatggctc gttatgtcat cttcctctat caggcgggcg ggaatctcac caacgccgag
       61 gtgaccgaga tcgaactcaa ctcggatgcc gcgaagacgg cgctggccga gatgcacggg
      121 ctctacagcg atggtctctc cgtcgcatcg gcagacatcg gcgcgggttg gcccggtgat
      181 gccctggccc agggactgac ctcgatcgcc ttcgagggca actggctctt cccgttcctc
      241 gtcaccaacg cgccggacct gaaatacgcc atcgccgaac ttcccgccgg gccggcaggc
      301 aaaggcacgc ccgccttcac gaattcctac tcgatcttca agggatcgaa gaatcccgac
      361 gcggcgtggg tgctggtgaa cttcctgacc ggccccgaag gcgccgagat cgccggcgag
      421 ctcggtctcg cgatcccggg acgcccggat ctcgaggcgg gctggctcaa gctcttcccc
      481 gatcgccagc cgtatatcgc ggcaggcaaa tacgccaccg ccgtccagta cgggccgggc
      541 ggccagaaat tccagactga tgccgacgcg atcctgcagt cgctctgggc gggccagatc
      601 gaagaatccg cggcgatcca gcagatctac gatacggcca agagcgatat caccttgacc
      661 ggcggccagt agcattccca cccggcaggt gaccgaagtc aggctgcgaa cgcgcgggag
      721 tcactcctcg cgcgttcgcc cgtaacggct tgggttccgc ccaggggaga aggttcatga
      781 tcgcgatacc tcgccggtgg aagccccgga atccgacggc cgccgaagat gcgatcgtcg
      841 gttggctctt catcgctccg gcgctcgttg tcatctctgt cttcgtcttc tggccgatcc
      901 tccagatctt ctggctcagc ttccaccaat ggagcgtgat cacgccagaa aagccctggg
      961 ttggtttgcg caactaccgc gacttcttca acaacccgga tttcgaaacg gcgctcagga
     1021 ataccctctg gtactcgatc ggcgtcgtgc cgatccagac cttcatcgcg gtctttctcg
     1081 ccgtcgtcgc caaccagaag atacgcggtc gcggtttctt tcgaaccgcc ttctacttcc
     1141 cttcgatcag ctccacggtc gtgatcgccg tgatcttcct ctggctctac cagcagaatg
     1201 gactgatcaa cttcacgctg cgcaagatcg gattctcgcc gccgcggcca ccgtggctcg
     1261 cgaatcccaa gggcgtgatc gagatggtgc tcaacggcgt cggcgtccat catgtcgacc
     1321 cctggttcgc cgggccaagc gtcgcgctcc tctcgatcat gatgatggcg atctggacct
     1381 ccgtcggcgg ctacatggtg atcttcctcg ccggtctgca ggacatcccc gccgaggtct
     1441 acgaagcggc cgcgctcgac ggagcgagca agcgtcagca gttcacccag atcacgctcc
     1501 cattgctccg accggtgatc ttcttcgtgg tgacgcttgg tttcatcggc accttccagg
     1561 tcttcgactc gatctacatc atggcccccg gtggttcccc cgcgaagacg acgctgaccc
     1621 tcggctacct cgtctaccag cagggcttcc agaatttcgc gatgggctac gcctcggcga
     1681 tcgcgatcgt cctcttcgcg atcatcctcg gcatctacct cttccagcgc cgctttctcg
     1741 gtcgcaccga ccaacggtag gaacgaatca gcatggctgt catcacatcg gaaacgatca
     1801 aggcgggaca aggcggcgag gtccggggca gacgctcgat cgggcagacg atcgtcgtca
     1861 ccatcgccta tgtgattctc gtcgcggttg ccctactcat gttcgtcccc ttcctcttct
     1921 cgctggccac ctcgttcaag acgccgccgg atgccctccg cctcaccttc tcctccatgt
     1981 tctggccccg aacctggacg ctcgatgcct atcgcacggc gctcgactcc aacatccagc
     2041 gctggttcat caacagcgcc gtcgtcgccg cgatctggat ctgcgga
//
LOCUS       DASTMD010000552         3050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2285062, whole genome shotgun sequence.
ACCESSION   DASTMD010000552 DASTMD010000000
VERSION     DASTMD010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2285062"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFQ54_08090"
     CDS             <1..889
                     /locus_tag="VFQ54_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0164990.1"
                     /translation="PQASPVTGGTPVAGSASPVASPTVVSSPVGTPNASPTPDPEQTA
                     DAQFTNFQNQVFSKAHMSLKDYYRLVAEPQLAKQKIDTTLQNATPQSQPQVHAYHILV
                     ATQDLAQQLYNKAKGGASFTELAKTNSTDTATAPTGGDLGWVVKSEIDPADADAVFAL
                     DVNGISQPTQTGYGWEIVKVVAKDQDRALTDAQYQDAQNKAVSDWLDQQRASTKIKTS
                     YDVTPTATADTYAPPSGAPTVPPATPIPTIPPPVLGPAYVPPGGTPMPAATPIGPEGT
                     PVSSPVPASTPIGTATAAA"
     gene            1017..2063
                     /locus_tag="VFQ54_08095"
     CDS             1017..2063
                     /locus_tag="VFQ54_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164991.1"
                     /translation="MSSRDRSDQPRPPRRSSDPTRSSQPKSDASGESWDIDPAEIDRY
                     LSGRPQRSETPAPARRQRVTPRRAASEPQSSDSGQSSTVDQIAQLRNRVAQDAAQRSS
                     STGSSGRTRPASPSTTRQSFDRSNLAPSQPTDEDIPLAFEPSRDDEPLGRRAATSASR
                     ERLLRSDPFANDIVVQSNEDAYAPFVDDAYDDEYDDDEDYVEERPRRERGPRRQLSAP
                     TMPKLSMPSLPNFTVPPRVAQAAIFDDVVSLSLIGIGAVSLAVMSILVANRADSLASP
                     FATHISASGVAEHLKTRGAIWSLPILGVFLMLMNIVIALFVARMDRFASRFVLGAGLV
                     VQIVAWVAVIRILW"
     gene            complement(2153..3007)
                     /locus_tag="VFQ54_08100"
     CDS             complement(2153..3007)
                     /locus_tag="VFQ54_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU0164992.1"
                     /translation="MFPSSFAYHRATSVADAVQILAANPAAKILAGGYSLIPAMKLRL
                     AMPEVLVDLGKLSELKAISIEEGQIRIGAMVTYDAIRDNAELVARFPVFPEAILYIGD
                     QQVRAHGTLGGALAHNDPAADLTAVFLALDGSVSVTGANGARTIASDELFVDLWTTTL
                     EPDEIITEISLPTPAAGTVMAYKKYGHPASGYALAGVAAVVAVENGLVTSARIGVTGA
                     TSTSTRATAAEESLIGQPLDDASIAADAALVPDGLEINGDTFADAEYRTHLLTVLGRD
                     VLKHAISR"
BASE COUNT          549 a         1059 c          943 g          499 t
ORIGIN      
        1 gccccaggca tctcccgtca ccggcggcac accggtcgcg ggttccgcat cgccggtcgc
       61 gtcgccgacg gtcgtctcat ctccggtggg gacaccgaac gcgtcgccga cacccgaccc
      121 cgaacagact gcggacgcgc aattcacgaa cttccagaac caggtcttca gcaaggcgca
      181 tatgtcactc aaggactatt atcgcctcgt cgccgagcct cagctcgcga agcagaagat
      241 cgacaccacc ctgcagaatg caacgccgca gagccagccc caggttcacg cctaccacat
      301 cctcgtcgcg acccaggacc tggcgcagca gctctacaac aaggcgaagg gcggcgcgtc
      361 cttcaccgaa ctggcgaaga ccaactcgac ggacaccgcg accgcgccga ccggcggtga
      421 tctcggctgg gtggtcaaga gcgagatcga cccggcggac gccgacgccg tcttcgcgct
      481 cgacgtcaac gggatcagcc agccgaccca gaccgggtac ggatgggaga tcgtcaaggt
      541 tgtcgccaag gatcaggatc gcgccctgac cgacgcccag taccaggacg cccagaacaa
      601 ggccgtctcc gactggctcg accagcagcg cgcctccacg aagatcaaga cgagctacga
      661 cgtcacaccg acggccaccg cggacaccta cgcaccgccg tccggcgcgc cgacggtacc
      721 gcccgccaca ccgatcccga ccatcccgcc gccggtcctc ggccccgcct acgtgccgcc
      781 tggtggaaca ccgatgccgg cagcgacgcc gatcggccct gaaggaacgc cggtttcgag
      841 cccggtaccg gccagcacgc cgattggaac ggccacggcc gccgcgtagc ggatcgacgt
      901 caccgaatgg caagccccgt ggttcacgtc acggagtttg ccatccgggg tgtgctatgc
      961 tgcccgatga tactgcgggg aatccccggg tcacccactg agaggagtct tcgatcgtgt
     1021 cgtcccgaga tcgttcagac cagcctcgtc ctccacgtcg atcctccgat ccgacgcgaa
     1081 gctcccagcc caaatcagac gccagcggcg aatcatggga catcgatccc gccgaaatcg
     1141 accgctatct ttccggtcgt ccgcagcgat cggagacgcc agccccggcc cgccggcaac
     1201 gcgtcacgcc gcgcagggcg gcctcggagc cgcagtcgag cgacagtggt cagtcgagca
     1261 ccgtcgatca gattgcccag cttcgcaatc gcgtcgccca ggatgccgcg cagcggtcat
     1321 cgtccaccgg aagcagcggg agaacgcgcc cggcgtcgcc gtcgacgacg cgtcagagct
     1381 tcgatcgttc caatctcgcg ccgtcgcagc cgaccgacga ggatattccc ctcgccttcg
     1441 agccgagtcg tgacgacgag ccgcttggcc ggcgagccgc gacgagtgcg agtcgtgagc
     1501 gcctcttgcg atccgatcct ttcgccaacg acatcgttgt gcagtcgaac gaggacgcct
     1561 acgctccgtt cgtcgacgac gcctacgatg atgaatacga cgatgatgaa gattatgtag
     1621 aggaacggcc gcgccgcgag cgtggtccga ggcggcaact ctcggccccg accatgccga
     1681 agctttcgat gccgtcgctg ccgaacttca ccgttccgcc gcgcgtcgcc caggccgcca
     1741 tcttcgacga tgtcgtctcg ctctcgttga tcggcatcgg ggccgtgagt ctcgcggtga
     1801 tgtcgatcct cgtcgcgaac cgggccgact ctctcgcctc accgttcgcg acgcacatca
     1861 gcgcgtccgg ggtcgccgaa cacctcaaga cccgcggggc gatctggagc ctgccgattc
     1921 tcggcgtgtt cctgatgctg atgaacatcg tgatcgcgct cttcgtcgcc aggatggatc
     1981 ggtttgcgag tcgcttcgtt ctgggcgcgg ggcttgtcgt ccagatcgtt gcctgggtcg
     2041 cggtgatccg catcctctgg tagcacgatt cccgttcacg cacgcaagag ccctggatcc
     2101 tccgaacgga ggatccaggg ctcttgcgtt tcgtccgaag gaacgctgcc cgttatcgag
     2161 aaatcgcgtg cttcaggacg tccctcccca gaaccgtcag caggtgagtc cggtactcgg
     2221 catcggcgaa cgtatcaccg ttgatctcga ggccgtccgg cacgagcgcc gcgtccgcgg
     2281 cgatgcttgc gtcatcgagc ggctgtccga tcagcgattc ctcggcggca gtcgcccgcg
     2341 tggacgtcga ggtcgcgccc gtcaccccga tgcgcgcgct cgtgacgaga ccattttcca
     2401 cggccacgac ggcggcaaca ccggcgagcg cgtatccgct ggccgggtgg ccatacttct
     2461 tgtaggccat cacggtcccg gcggcaggcg tcggcagact gatttcggtg atgatctcgt
     2521 ccggttcgag cgtcgtcgtc cagaggtcga cgaagagctc gtcggaggcg atggttcgcg
     2581 ctccgttcgc gccggtcacg ctcacggagc cgtcgagcgc aagaaagacc gcggtgaggt
     2641 cggcggcagg atcgttgtga gcgagggcac cacccagcgt gccgtgcgcg cgaacctgct
     2701 ggtcaccaat gtagaggatc gcctccggaa agaccggaaa gcgagcgacc agttcggcgt
     2761 tgtcgcggat ggcgtcgtag gtcaccatcg cgccgatccg gatctggccc tcctcgatcg
     2821 agatcgcctt gagttcactg agcttgccga gatcaacgag aacctccggc atcgccagac
     2881 gaagcttcat ggccggaatc agcgagtatc cgccggcgag aatcttcgcc gccggattcg
     2941 cggcaaggat ctggacagca tcggcaaccg acgtggcgcg atggtatgcg aaggaagatg
     3001 gaaacacgat cccgtcccgt cacttacgcc ttgcggtcgg cgttcatctt
//
LOCUS       DASTMD010000553         1788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1927707, whole genome shotgun sequence.
ACCESSION   DASTMD010000553 DASTMD010000000
VERSION     DASTMD010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927707"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VFQ54_08105"
     CDS             complement(<1..1038)
                     /locus_tag="VFQ54_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEU0164993.1"
                     /translation="MAIAQRTTPALNPITVMSALRSPRRLSREVLAGMVTTLALVPEV
                     ISFSIVAGVEPMVSLVASIVLAITMSILGGRPGVITAAAGSVALVIAPLVHQHGVQYV
                     LPTVILAGLVQIVFGFAGLARLMRYVPRSVMIGFVNALGVLIFTAQVRHIVNVPWLAY
                     VLFGVTFAIILIVPRFTKAIPSPLIAIVVVTAVVIITHVVVPNVADEGPLAGKLPGFT
                     QLVVPLNLTTLQMIWPTVVSIAFVGLLETLLTAKLVDEITDTPSHKGRESWALGISNI
                     LAGCYGGIAGCAMIGQTLLNVKIGRARTRLSTVVAGVFLLVLVTGLSPIMGQIPMVAL
                     AAVMMVVGITTV"
BASE COUNT          366 a          548 c          623 g          251 t
ORIGIN      
        1 gacggtggtg atgccgacga ccatcatgac cgcagccagg gcgaccatcg ggatctgtcc
       61 catgatcggg ctgagtccgg tgaccaacac gagcaggaag acgccggcga cgacggtgga
      121 aaggcgcgta cgggcgcgcc cgatcttcac gttcagcagc gtctgtccga tcatcgcgca
      181 accggcgatg ccgccgtagc aaccggcgag gatgttggag ataccgagcg cccaggactc
      241 ccggcccttg tgagatggcg tgtcggtgat ctcgtcgacg agctttgccg tcagtagggt
      301 ctccagcagg ccgacgaaag cgatactgac gacagtcggc cagatcattt gcagcgtcgt
      361 gaggttcagt ggtactacga gctgagtgaa acctggcagc tttccggcga gaggtccttc
      421 gtcggcgacg ttcgggacga cgacgtgggt gatgatcacg accgccgtga cgaccacgat
      481 cgcgatcagc ggcgacggga tggccttggt gaacctcggg acgatgagaa tgatcgcgaa
      541 cgtcacgccg aacaggacat aggcgagcca cggaacgttc acgatgtggc gcacctgggc
      601 ggtgaagatc agcacgccca gcgcgttgac gaacccgatc atcaccgagc gcggtacgta
      661 gcgcatcagg cgtgccagac cagcgaatcc gaagacgatc tgaacgagac ccgcgaggat
      721 caccgtcggg agaacgtatt gcacgccgtg ttgatggacc aggggcgcga tgacgagggc
      781 gaccgatccg gccgcggcgg tgatgacacc cggccggccg cccaggatcg acatcgtgat
      841 cgccagcacg atcgatgcga cgaggctgac catgggttcg acgccggcca cgatcgaaaa
      901 cgagatcacc tccgggacaa gcgccagagt cgtcaccatc ccggcgagga cctcgcggga
      961 caggcgccgc ggcgagcgca gtgcgctcat cacggtgatc gggttgaggg cgggcgtggt
     1021 gcgttgggcg atggccaagg agcgactccg ggagacggct ggttcgtgag tggcgggttg
     1081 ggaagtgaac gtcacactgc gcaagcagtc cgccccggtc ttgctccgat cggcagagga
     1141 gcgaccgggc ggaaagtcac cgggaggcgc gtgatgactt tactcgaaca tcgccatcga
     1201 gcaggcgcgt ggtgtgacgg cgaaggaggc accgccgcgg aggccgtcgc aacgttgcag
     1261 gtcagcgcgc atccagtcgg tgctcagcgg tcgctgcgga ccctccgcca cgagggcggg
     1321 gccagagtcc agaacccgtc aggcccggct gggtgcttca gggagtgcct acgtcgatca
     1381 acgaccgcgg cgaccgcttc gagttccacc agaaggttgt cgcacaacgg agtgcatcgc
     1441 gcgagtcgca ttgagcggtc gagccaccag gacaagcagg cggccattcc cagtgagccg
     1501 tcaccagcca gtagccagtt gcggcacgga gacctgaagc cgcacccctg tcccacggag
     1561 agcggccgac cctacgagca ggccccgaac caccccgcca agaacgtaag gaaggagatc
     1621 tcgtgatcgg gtcggaacgt tccgacgagc ggacgccgcg attcacgatc ctcgtgcgct
     1681 tgccccgcaa ggagcgctag tgccacaagg agagacccag gaacgggccg gcgctgggcg
     1741 gctacggcac gatcaggatg caggacgccc tcgccaccac gatgtcga
//
LOCUS       DASTMD010000554         1733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1927918, whole genome shotgun sequence.
ACCESSION   DASTMD010000554 DASTMD010000000
VERSION     DASTMD010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927918"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..741)
                     /locus_tag="VFQ54_08110"
     CDS             complement(148..741)
                     /locus_tag="VFQ54_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164994.1"
                     /translation="MAVRPGGGERRSCRAAIFMFLILALGLPQIASAQSSAEISVSVV
                     PPSGGALSATLVSSSFAVVNHPPERSNRIVKGTIKVLVEDSTGSGRPWNVRASAAANL
                     VSDDGEAIPATGLVLTVPASNGLTRSTNVGNIPTPSHVIVFDTGKPISLLACAKGNCQ
                     GAFVVTFSATLSVPDWAPVGTYSSTLVLEIAAEPDLS"
     gene            complement(807..>1733)
                     /locus_tag="VFQ54_08115"
     CDS             complement(807..>1733)
                     /locus_tag="VFQ54_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0164995.1"
                     /translation="TERPSPTPTLVPSPSPSPSSTAVPVISWSLAAPIDCRPVDSTGT
                     VASGGSIGYVCTVTMIVRGEVHAGMGLTSSWTLDVSASPGWSALVVDADGQPRGDGRT
                     GVAISTPVSIGGGQVANPVTTSFGLQVVRDSCTTTSADVAIAAQGKLAGSGVSIQAPS
                     DAVAAASLEPGLRAIPEPSIILAGPLELGVVNLSPDGSTSDTSGTIDLRISGLDQACG
                     TWTILVTGGALSNDAGATIAAGNLTLRSVNGVPAGGGSCRLDGGCVVATVTAGPGSSP
                     VIDLVLGIGLQVPIGTDVGSFTTTLQASIAAN"
BASE COUNT          326 a          577 c          543 g          287 t
ORIGIN      
        1 ttttctctcc gccgacaaca tagtactatc gtcctagtct ctcgtcaata ccctatcaga
       61 ccaaagtcac gttttgccta gtaccggggt cgacagggct agtcctcggt ccacatgtct
      121 ggcatccacc gacgggatcg ggctcgacta cgacagatct ggctcggccg cgatctcgag
      181 gacgagcgtt gagctgtagg ttccgaccgg cgcccaatcg ggaacgctca gcgtcgcaga
      241 aaaggtcacg acgaatgccc cctggcagtt tcccttcgca caggcaagga gcgaaatcgg
      301 tttgccggtg tcaaacacga tcacgtgtga aggcgtgggg atgtttccga cgttcgtcga
      361 gcgggtcagc ccgttcgatg ccggaacggt gaggacgaga ccggtcgcgg ggattgcctc
      421 gccgtcatca ctcacgagat tcgccgccgc tgacgcccgc acgttccacg gacgcccgga
      481 tcccgtcgaa tcctcgacca gcaccttgat cgtccccttc acgatccggt tcgatctttc
      541 tggcgggtgg ttcacaacgg caaacgatga ggaaaccagg gtagcggaaa gcgccccgcc
      601 agaaggcggc acgacgctca cactgatctc cgcggatgac tgtgcgctcg cgatctgggg
      661 gaggccgagc gccagaatca ggaacatgaa gatcgcggcc cggcatgatc tcctctcgcc
      721 tccgcccggt ctgactgcca tcctcaccga atactccttt ggctcgatcg cgaccgtgtc
      781 tgcgaatccg gggcgcgatc gctcgctcag ttcgcggcga tggacgcctg aagcgtggtc
      841 gtgaacgacc cgacgtcagt gccgatcggc acctggagtc cgatcccgag cacaagatcg
      901 atgaccggcg agcttcccgg cccggccgtg accgtcgcca cgacgcatcc cccgtcgagc
      961 ctgcaggaac cgccgccggc aggaacgcca ttcacggagc ggagcgtgag attgcccgcc
     1021 gcgatcgtcg cgccggcatc gttgctcaat gcaccgcccg tgacgaggat cgtccacgtc
     1081 ccacaggcct gatcgagacc ggaaatccgg agatcgatcg tgcccgaggt atcggaggtg
     1141 ctcccatccg gggagagatt gacaactcca agctcgagcg gtccggccag gatgatcgat
     1201 ggttcgggga tggcccgaag accgggctcc aggctggccg cggcgaccgc atccgacggc
     1261 gcctggatgg acaccccgga acccgcgagc ttcccctggg ccgcgatcgc gacgtcggcc
     1321 gacgtcgtcg tacagctatc ccgcacgacc tgcaatccga acgaggtggt aaccgggttc
     1381 gcgacctgtc ctccaccgat cgaaaccggc gtagagattg cgacgccggt ccgaccgtcc
     1441 cctcggggct gaccgtctgc atcgacgacc agggcgctcc agccgggcga ggcgctgacg
     1501 tcgagcgtcc agctgctcgt caatcccatg ccggcatgga cttctccccg gacgatcatc
     1561 gtcacggtgc agacgtaacc gatcgaccca ccactcgcca cggtacccgt cgaatcgacc
     1621 gggcggcagt cgattggcgc cgccaacgac caggagatca cagggacagc cgtcgacgac
     1681 ggcgatggtg acggggaagg aacaagggtt ggggtcggtg agggccgttc ggt
//
LOCUS       DASTMD010000555         4603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2195984, whole genome shotgun sequence.
ACCESSION   DASTMD010000555 DASTMD010000000
VERSION     DASTMD010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195984"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VFQ54_08120"
     CDS             <1..680
                     /locus_tag="VFQ54_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU0164996.1"
                     /translation="IGLERERLERAAGLRTHALVALASALVMIVSAYGFADVLVAGES
                     TVLDPSRIAAQVVSGVGFLGAGVIILRKNTVRGLTTAASVWTVAGIGLACGGGLLAIA
                     VVATLSVLIIQAVVRPMERRFFDHHSPQQIVMRVQRAPGRLAEVEASVAASRAALRGL
                     RLRPARGGAEDRVELDLTGASSRQVSELIDALRCLDGVQLVEYTLDSPQSSRAERIAS
                     NEREDEE"
     gene            complement(739..1125)
                     /locus_tag="VFQ54_08125"
     CDS             complement(739..1125)
                     /locus_tag="VFQ54_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0164997.1"
                     /translation="MYRDPLVNFYVADVDASVRFYTELFGFEETFRTPATGRPDHVML
                     RLGGLMLGLADFEAAKRDHGLPAAPGPTRAEVAIWTDDVDAAYADLIANGAGPLTPPH
                     DFIGTLRAAWVLDPDGGPIQIVMRLS"
     gene            1234..1857
                     /locus_tag="VFQ54_08130"
     CDS             1234..1857
                     /locus_tag="VFQ54_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU0164998.1"
                     /translation="MRPARGFSGTDARTQRERGETITTLENRPHTALIVIDVQNGVVA
                     GTHERDAVVANIGGLVEKARREQVPVVWVQQTDERLAKGNDIWQIVPELTPDAADPHI
                     EKYYGDSFEETALENVLSGLGVGRLIVVGAQTDACIRATLHGALVRGYDTTLVSDAHT
                     TEDQTAWGAPPPELVIAHTNLYWSEETAPGRTAGTVTTGDVSFDGTS"
     gene            complement(1933..2391)
                     /locus_tag="VFQ54_08135"
     CDS             complement(1933..2391)
                     /locus_tag="VFQ54_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0164999.1"
                     /translation="MDTTTSIRDLPERTCGIRAALDVVGDRWTLLIVREVLFSNRRFS
                     GIADRIGVPRDRLAARLKLLVETGVLEQREYQTAPPRSEYHLTESGRGLAPVLATLFE
                     WGKRWGHVSDDGIELRHHDHEAQPALMCEICGEPVHADDLSRREVAVGKD"
     gene            2508..3965
                     /locus_tag="VFQ54_08140"
     CDS             2508..3965
                     /locus_tag="VFQ54_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165000.1"
                     /translation="MSPIPVPESLPASSAPRSLPIFLVICTAVFMTNLDLRVVNVALD
                     AIGRDLAGQSLSGLSWVINGYTVALAALLIVAGRLGDRSGLRRLFLIGTVIFTIASLG
                     CALAPNLGILVAVRVLQATGAAILLPASLSLLLHGVPAERRHTAARIWSAVGALAAAS
                     GPALGGLLIEASWRWVFVINVPIGIAVVFFGFRILADDVAQRDASPRQPLPDVIGSGL
                     LAAGVAALTGAIVQGPDWGWTSGRVIGLAALALVALIWFFQRCATQPVPLIELNLWRI
                     RAFAIANVGSFLFSISFAIMLLSNSLWCQGIWGYSALMTGLAMAPGPAVVPFVAIASN
                     RAIGRYGAGIVIAIGSLLFAAAQIWRVTQASASGSYAWDLLPSLILSGIGTGLAFSTM
                     IASGSTALPANRSATGAAVLNTGRQIASAIGVAILVAALGNSALGPQSVHAFHVAWGI
                     SAVLVAAAAVVTFFLPRDRVASDAPERSPVLATAE"
     gene            complement(4114..>4603)
                     /locus_tag="VFQ54_08145"
     CDS             complement(4114..>4603)
                     /locus_tag="VFQ54_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0165001.1"
                     /translation="YSILNRGIEREVLPVAQQYGMGILVWGPLGQGMLTGRVRRGQDS
                     DVIRAKFLTAFSDERRLDAVEQLIPLAEEVGLSMPHLAMAFAISHPGVTSALLGARTM
                     EQLDDLLAGMDVALTDDVLDRIDEIVPPGTDVGTLDQAYVPPAIQRTRLRRRPIQERS
                     AS"
BASE COUNT          717 a         1592 c         1482 g          812 t
ORIGIN      
        1 tcatcggtct cgaacgggaa cgcctggaac gcgccgccgg tctgcggacc catgcgctcg
       61 tcgccctggc ctcggcgctc gtcatgatcg tctccgccta cggtttcgct gacgtactcg
      121 tggcgggaga atcaaccgtg ctcgacccga gccgtatcgc cgcccaggtc gtgagcggcg
      181 tcgggtttct cggcgccggc gtcatcatcc tccgcaagaa cacggtgcgc ggtctgacga
      241 ccgccgcgag tgtctggaca gtcgcgggga tcggactcgc ctgcggtggt ggactgctcg
      301 cgattgccgt cgtcgccacg ctgtccgtat tgatcatcca ggcggtggtg cgcccgatgg
      361 aacggcgctt cttcgatcat cactcaccgc aacagatcgt gatgcgcgtc cagcgcgcgc
      421 cggggcgact cgccgaggtg gaggcgagtg tcgccgcgtc gcgggcggcg ctgcggggtc
      481 ttcggctgcg tccggcacgc ggtggcgccg aagaccgcgt cgagctcgat ctgaccgggg
      541 cgtcgtcccg gcaggtaagc gagttgatcg atgcccttcg gtgcctggat ggggtccagc
      601 tcgtcgaata cacactcgat tctccccagt cgtcaagagc ggagaggatc gcatcaaacg
      661 agcgagagga cgaggaatga ggtacctggt ccacgatgcc cggccggttg cgctcgtcct
      721 cgagatgcgt ggcccgattc aggaaagacg catcacgatc tgaatcgggc cgccgtcggg
      781 gtcgaggacc caggccgccc gcaacgtgcc gatgaaatcg tgcggcggcg tgagtggccc
      841 ggcgccgttc gcgatcaggt ccgcgtaggc cgcatcgaca tcgtcggtcc agatcgcgac
      901 ttccgcgcgc gtcgggccgg gcgcggcggg taatccgtga tcccgcttcg ccgcctcgaa
      961 gtcggccagg ccaagcatca acccaccgag tctcagcatc acgtgatcgg gtcgaccggt
     1021 ggcgggtgtc cggaacgtct cctcgaatcc gaacaattcg gtgtagaacc gtacggacgc
     1081 gtcgacatcc gcaacgtaga agttcaccaa cggatcgcgg tacatggaaa ccacctcttc
     1141 cagatgcgaa aaccgacggg ccctgccggc aggcggcttg acagggttgc cgatgccccc
     1201 tggctcgaga gctacactat tcccggatat cgcgtgcgcc cggcgagagg cttcagcggg
     1261 acagatgcac gaacccagcg tgagcgaggg gaaaccatta ccacactcga gaaccgacca
     1321 cacaccgccc tcatcgtcat cgacgtgcag aatggcgtcg tcgcaggcac gcacgaacgc
     1381 gacgcggttg tcgccaacat cggcggcctc gtcgagaagg cgcggcggga acaggttccc
     1441 gtcgtctggg tccagcagac cgacgagcgg cttgccaagg gcaacgacat ctggcagatc
     1501 gtccccgaac tgacacctga cgccgccgat ccgcacatcg agaagtacta cggtgactcc
     1561 ttcgaggaaa ccgccctcga gaacgtgctg tcaggtctcg gcgtcggtcg cctcatcgtc
     1621 gtcggcgcgc agaccgatgc gtgcatccgc gccacgcttc acggcgctct cgtcagagga
     1681 tacgacacca ccctggtcag cgacgcccac acgacggagg accagacggc atggggagca
     1741 ccgccgccgg agctggtcat cgcgcacacg aacctgtact ggtccgagga gacggcacca
     1801 gggcgaacgg ccgggacggt cacgaccggg gacgtcagct ttgatggcac gtcctgaagc
     1861 cgacgtgatg cgaaggaaac cacgtgccga cacccgccgc tcgcccgtca ccaatccgac
     1921 gaccgcatac agctagtcct tgccgacggc gacctcgcgc cggctcaggt catcggcatg
     1981 cacgggctca ccgcatatct cgcacatcag tgcaggctgc gcctcgtgat cgtgatgccg
     2041 caattcgatg ccgtcatcac tcacatgtcc ccatcgtttt ccccattcga agagcgtggc
     2101 cagcacgggc gccagtcccc gtccggactc ggtgaggtgg tattcggaac gtggcggggc
     2161 cgtctgatat tcccgctgtt cgagcacgcc ggtctcgacc agcagcttca ggcgcgccgc
     2221 gaggcggtcc cgcgggaccc cgatccgatc cgcgatccca ctgaagcggc ggttgctgaa
     2281 gagcacctca cgcacgatca gcagcgtcca gcgatccccg acgacatcca gcgcggccct
     2341 gattccacat gtgcgctccg ggagatcccg gattgatgtt gttgtatcca tggcgatcat
     2401 cctagcgcga gtcagtttga ttttcaatct gtctcctgat aggatgccgg ggagtcggtt
     2461 tgtgattcaa acccatgcct tgatccagtc gagaaggtcg ccccacgatg tcaccgatcc
     2521 cggtccccga gtcacttccc gcatcgtcgg cgccacgatc gctgccgatc ttcctcgtga
     2581 tctgcaccgc cgtcttcatg acgaatctcg atctccgggt cgtcaacgtc gccctcgatg
     2641 cgatcggtcg cgatctcgcg gggcagtcgc tctccgggct ctcctgggtg atcaacggct
     2701 acacggtcgc actcgccgca ctgctgatcg tcgccggtcg gctcggggat cgctcgggac
     2761 tccggcggct cttcctgatc ggcaccgtca tcttcaccat cgcctcgctc ggttgcgcgc
     2821 tggccccgaa tctcggaatc ctcgtcgcgg tgcgcgttct ccaggcgacc ggcgccgcga
     2881 tcctcctgcc ggcatcactc tcgctgctcc tgcacggtgt gccggcggag cgtcgccaca
     2941 ccgcggcccg gatctggtcc gccgtcgggg cgctcgccgc ggcgagtggc cccgcgctcg
     3001 gcggtctcct gatcgaggcg agctggcgct gggtcttcgt gatcaacgtg ccgatcggga
     3061 tcgccgtggt cttcttcggc ttccgcatcc tcgccgatga cgtcgcgcaa cgcgatgcgt
     3121 cgccacggca accactgccc gacgtgatcg gtagcggcct cctcgccgcc ggcgtcgcgg
     3181 cgctgaccgg cgcgatcgtc cagggaccgg attggggctg gacgtcgggc cgggtgatcg
     3241 gcctcgcggc cctcgcgctt gtcgcgctga tctggttctt ccagcgatgc gcgacgcaac
     3301 ccgtccccct gatcgagctc aatctctggc ggattcgcgc cttcgcgatc gcgaacgtgg
     3361 gcagcttcct cttctcgatc agcttcgcga tcatgctgct ctcgaactcg ctctggtgcc
     3421 agggaatctg gggctactcc gccctgatga ccggtcttgc gatggcgcct ggtccggccg
     3481 tggtgccgtt cgtcgcgatc gcctcgaacc gcgcgattgg ccgctacggt gccgggatcg
     3541 tgatcgcgat cggcagcctc ctcttcgccg ccgcccagat ctggcgggtg acgcaggcaa
     3601 gcgcgtccgg ttcgtatgcg tgggacctcc tgccctcgct gatcctgagt ggaatcggca
     3661 ccggactcgc cttctcgacg atgatcgcct ccggctcgac cgcgctgccg gcgaaccgct
     3721 cggcgactgg cgccgccgtc ctcaacaccg gccggcagat cgcttccgcg atcggcgtcg
     3781 cgattctcgt cgccgcgctc ggaaactcgg cgctcggccc gcagagcgtg cacgccttcc
     3841 atgtggcgtg gggaatctcc gcggtactcg tcgcggcggc ggcagtcgtc accttcttcc
     3901 tgccgcgcga tcgggtcgcg agcgacgcgc cggaacgatc gcccgttctg gcgacggcgg
     3961 agtagggcgt gtcgccaatc cgtccttgtt tcccgcgacg aagccgaccg gcggcccacc
     4021 ggtcgactcg acggacggtc gtgtaagcgg tgccgtggcg acccttcgcc gcacgtcacg
     4081 cttcttctgg acacggccat ctcggcctcc ggttcaggac gctgaacgct cctggatcgg
     4141 gcgccgccgc aaccgcgtcc tctggatcgc cggtggcacg taagcctggt cgagcgtgcc
     4201 gacgtcggtg ccgggcggca cgatctcgtc gatacggtcg aggacgtcat ccgtgagagc
     4261 gacatccatc cccgcgagga ggtcgtcgag ctgctccatc gtgcgagcac cgagcagggc
     4321 gcttgtgacg cccggatgcg agatcgcgaa cgccatcgcg aggtggggca tcgacagccc
     4381 cacttcctcg gcgagcggga tcagctgctc cacggcgtcc aggcgtcgct catcactgaa
     4441 cgccgtgagg aacttcgcgc gaatgacgtc gctgtcctgc ccgcgacgga cccggccggt
     4501 gagcatgccc tgcccgagtg gcccccagac gagaatgccc atgccgtact gctgcgcgac
     4561 cggaaggact tcacgttcga tgccgcggtt caggatcgag tat
//
LOCUS       DASTMD010000556         6516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2108453, whole genome shotgun sequence.
ACCESSION   DASTMD010000556 DASTMD010000000
VERSION     DASTMD010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108453"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..702
                     /locus_tag="VFQ54_08150"
     CDS             160..702
                     /locus_tag="VFQ54_08150"
                     /inference="COORDINATES: protein motif:HMM:NF002598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyuridine 5'-triphosphate
                     nucleotidohydrolase"
                     /protein_id="HEU0165002.1"
                     /translation="MEELENLGGVLGREQLRALIEAPERPLLTEWQDIDQQIQPNGVD
                     LTLREISAYRGRGQIGVSSEDRILPELEPLEFDADGWVTLAPGIYQIVYNEIVDIPVT
                     LMALGRPRSSLPRGGATIHTAVWDAGYRGRSTSMLSVLNPDGFRLQRNARVMQLVFFG
                     MSEAPVDGYRGKYQNENVGS"
     gene            complement(813..1091)
                     /locus_tag="VFQ54_08155"
     CDS             complement(813..1091)
                     /locus_tag="VFQ54_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165003.1"
                     /translation="MLSFLRRKPEQSPVPVCPDHKVERRIRGKLGRPSRFTDQTEEEY
                     TLIFYCPEEKCNQTAMRVQTRSQIPVPGEPPRRPDYARIGDRNLTGDR"
     gene            1222..1677
                     /locus_tag="VFQ54_08160"
     CDS             1222..1677
                     /locus_tag="VFQ54_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165004.1"
                     /translation="MSSDDSTLPERVFDELVDVLIERYGEEWMTDTTANQAFSRGTAS
                     LLHDADGVHLIGEPEYEQKSHNFNLWVDFPVEDVYAADELAFNLFSHIAEDIFLSTRV
                     FEDKGVRYRFVTGTAEDGHLGSFNLTGPHAMEFVNIHKLRVSRGLQFNA"
     gene            1759..2021
                     /gene="ffs"
                     /locus_tag="VFQ54_08165"
     ncRNA           1759..2021
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFQ54_08165"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(2281..2733)
                     /locus_tag="VFQ54_08170"
     CDS             complement(2281..2733)
                     /locus_tag="VFQ54_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165005.1"
                     /translation="MDKLTSWDYLTTAPKEADVLGKYPVAYAVAFVLGFIIASFVYYR
                     PRTPFLSKYIRRKALGRLAFAGMWVFGLGLFFFLIRILVINPFTFAEPVWMVAMMLIA
                     AVLIVISAVVLFVALRRGPIPRASTGWTQSGYHAAPRRRPVQRRRALR"
     gene            complement(2801..3499)
                     /locus_tag="VFQ54_08175"
     CDS             complement(2801..3499)
                     /locus_tag="VFQ54_08175"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEU0165006.1"
                     /translation="MEVTLRIKRFNPEQDVKAHFEEYQLEVEPLDRVLDALNQVKWHQ
                     DGTLTYRRSCAHGICGSDAMRINGRNRLACKMLMKDLGKKITIEPLIGFKVEKDLLVD
                     MEEFFDKYKSVKPFLIADDAPSSGERRQSPEQRHVFDDTTKCILCAACTTACPVTWMS
                     DDFVGPAAIVNAHRFIFDSRDTGGRERLRILNSGSGVWRCRTIFNCTDACPRDIKITE
                     AIEQVKRALLFEGV"
     gene            complement(3551..5281)
                     /gene="sdhA"
                     /locus_tag="VFQ54_08180"
     CDS             complement(3551..5281)
                     /gene="sdhA"
                     /locus_tag="VFQ54_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEU0165007.1"
                     /translation="MAYHRYDAVIVGAGGAGLMAAIQLAGKANIAVVSKLYPPRSHTG
                     AAQGGISASLGNREEDHWEWHMYDTVKGGDYLVDQDAAEVLAREAVDAVLELEHMGLP
                     FNRTEDGKIDQRFFGGHTRNYGEAPVRRACYAADRTGHMIIQTLYQQGIKHNVNFFDE
                     HHLVDILFTDDGRCCGVVTYEIKTGEIHTIHAKTVLIASGGYGRVFKITSNAIAGTGD
                     GIALAYRRGIPLMDMEMYQFHPTGLYKLGILVSEAARGEGGKVINGLGEAFAARYAPT
                     LKDLAPRDMMSRFIYQEVKEGRGIDGKDYVFLDVSHLPPEVIDEKIPDVTDFARVYLG
                     VEPKKEGIPIQPTAHYAMGGLPTNINAQVIFSPDGQVVPGFYSAGESACVSVHGANRL
                     GTNSLVDLVVFGRRAGRAMLQEIQETDFAMLPDDPEYMAKSQIASLFDRTKGENAADI
                     RAEMQEVMMDKVSVVREENGMREAMSKIRELKSAYNAVAIQDHGKRYNTDLLETLELG
                     NMLDLAETIIHGAIERTESRGAHYREDYQARDDQNWLAHSMIYQTSGGLELKKKPIVI
                     TKFEPKERKY"
     gene            5595..>6516
                     /locus_tag="VFQ54_08185"
     CDS             5595..>6516
                     /locus_tag="VFQ54_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0165008.1"
                     /translation="MTVQSPELIANVSKIAESLVEEIVALTTKITEIAAPTNDEADRA
                     AFVHELFAEQGYLDVATDGLSDVVGRLPGRDSSAAPLLLAAHTDTVFPRSVPIKVARS
                     GDQIAAPGVGDNTLSVATVALLGKALKQLGVTPATDVYLTGNVGEEGLGDLRGMRAVV
                     DAIPNLGAAIAVEGHSLGRITYRAVGSKRLKVTVTGPGGHSWGDAGRPSAIHEMAKLI
                     AKLDEIPLTAEPKTSFNVGLIEGGYSVNTIAAQATCVIDMRSTSQESLANIVEKVETT
                     IARASKKEVDVVFEVVGDRPAGAMPLDSGIV"
BASE COUNT         1159 a         1981 c         2096 g         1280 t
ORIGIN      
        1 ggcgggacat agaagtcccg ccctacgaac gaccgcgcca gctgaatcga cagttcgtga
       61 acaacattcg gcgtcggaaa gaccgggttt gtaaggcggg agcttgtctc tcgccgcgaa
      121 catgacccgc cgcatgcgca cagatggaga gtcgacctgg tggaagagct tgagaatctt
      181 ggtggtgtac tcggtcgaga acagctgcgg gcgctgatcg aagcgccgga gcgcccgctg
      241 ctcaccgaat ggcaggatat cgatcaacag atccagccga acggcgttga tctgacgctg
      301 cgtgagatca gcgcctaccg cggtcggggg cagatcgggg tctcgtccga agatcgaatt
      361 cttcccgagc tcgaaccgct cgaattcgac gccgacggct gggtgacgct ggctcccggc
      421 atctatcaga tcgtctacaa cgagatcgtc gatatcccgg ttacgttgat ggcgctcggg
      481 cgacctcgtt ccagtcttcc ccgtgggggg gcgacgattc acaccgcagt gtgggacgcc
      541 ggctatcgcg ggcgctcgac gagcatgctt tcggtgctca atccggacgg attccggttg
      601 cagcgaaacg cacgcgtgat gcaactcgtt ttcttcggca tgtcggaagc cccggtcgat
      661 ggctatcgcg gtaaatacca gaacgagaac gtcgggagct gaccccgacc agccggtcag
      721 ccctggcgca tcaccggtgg gtcgatcatg cccggcgttg ccggacgggt cacatgggtg
      781 acccgtggga ctgttgtctg tccttctccg ggctagcggt cgccggtgag gttccggtcg
      841 ccgatgcgtg cgtaatcggg ccggcgtggc ggttcacccg ggacggggat ctgggatcgc
      901 gtctggacgc gcatcgccgt ctgattgcac ttctcctccg ggcaatagaa gatcagcgtg
      961 tattcctctt cggtctgatc ggtgaatcgg ctcgggcgac ccagctttcc acgaatccgc
     1021 ctttccacct tgtgatccgg gcagacgggg accggggatt gctctggctt gcgacggagg
     1081 aagctcagca ttcaccattc tccagcgaag ggttatcaga cgtgtcggtt ccttgttagt
     1141 atacgggatc acaaacgcgc cgcacgccgc gaaggcgacg ggcgcttcga ctcccccagg
     1201 gattccaagg agcaaccaga catgagttcc gacgactcga cactaccgga gcgggtcttc
     1261 gatgaattgg tcgacgttct catcgaacgc tacggtgaag agtggatgac cgataccacc
     1321 gccaaccagg ccttctcgcg cggcaccgcg tctcttctcc acgatgccga cggcgttcat
     1381 ctgatcggcg agccagaata cgagcagaag tcgcacaact tcaacctctg ggtcgacttc
     1441 ccggtcgagg atgtctacgc cgcggacgag ctggcgttca acctcttctc gcacatcgca
     1501 gaggatatct tcctctcgac ccgtgttttc gaggacaagg gggtccgcta tcgcttcgtg
     1561 acgggaaccg cggaggatgg acacctcggc agcttcaatc tcacggggcc ccacgcgatg
     1621 gaattcgtga acatccacaa gctgcgagtt tcgcggggac ttcagttcaa cgcctgatgt
     1681 cccaaatgtt gggcaatgat gaacgatggg gagcaggatt cctgtgcgtc tcgttcagaa
     1741 caggtatact ctccgcagtc gtgctagatg gggagctggc ggtgccctga acccgcaacc
     1801 cgccttagcg gggttgaatt ccctctcgag gtattggttt tgtgggacta gcgtcgtcac
     1861 gtggtgttga agaatgggtc tcgcgcggcg gggaactgtg aaccgggtca ggaccggaag
     1921 gtagcagccc taagcggcac tcttcgggtg acgcgaggtc gcctattccg agctggcgaa
     1981 cggcgaaaag ctggaagatc ttatcgacag agggtgcacg gctactcttt tgcactgtcg
     2041 acgcaacgca gatccggggt gatggatttc catcaccccg gatctgcgtt tattcgtggg
     2101 gatggcgctg ggctccagcg gagcgcgcga cggctgcaac ggttgtcaga cctgccgccg
     2161 ctcggatgcg acgccccgcc ggacatccgg tcgttcgcag ggcgggaccg ccgttgccgg
     2221 accgcagtcc ggacggcctg tcccgcctcg tgttcttgca acggattcga tcgccggacg
     2281 ctagcggagc gcgcgacggc gctgaaccgg gcgccgtcgt ggcgcggcgt gatagccgct
     2341 ctgtgtccag ccggtgcttg cccgaggaat cggcccgcgg cgcaacgcga cgaagaggac
     2401 gacggcggag atcacgatca ggacggcggc gatcagcatc atcgcgacca tccagacggg
     2461 ctcggcgaag gtgaacgggt tgatcacgag aatgcggatc aggaagaaga agagaccgag
     2521 cccgaacacc cacattcccg cgaacgcgag tcgaccgagc gccttgcggc ggatgtactt
     2581 cgagaggaac ggcgtgcgcg gccggtaata gacgaacgat gcgatgatga atccgagcac
     2641 gaaggcgaca gcgtaggcga ccgggtactt cccgagaacg tccgcttcct tcggtgcggt
     2701 cgtgaggtaa tcccacgatg tcagcttgtc catgcccacc tacatcttca acgtgtcgca
     2761 gcccgcgtcg actgccgatc gactcgcaag gacccggatt ctagacgccc tcgaagagca
     2821 gcgcgcgttt cacctgctcg atggcctcgg tgatcttgat atcacgcggg caggcatcgg
     2881 tgcagttgaa gatcgtgcgg caacgccaga cgcccgatcc cgaattgagg atgcgcagtc
     2941 gctcgcggcc accggtgtcg cggctgtcga agatgaagcg atgcgcgttg acgatcgcgg
     3001 cgggaccgac gaagtcgtcg gacatccagg tcaccgggca cgcggtcgtg caggcggcgc
     3061 agaggatgca cttggtcgtg tcatcgaaga cgtgacgctg ctccggcgac tgccggcgct
     3121 cgccactcga gggtgcatca tcggcgatca ggaacggctt gaccgacttg tacttgtcga
     3181 agaactcctc catgtcgacg aggaggtcct tctcgacctt gaagccgatc aatggctcga
     3241 tcgtgatctt cttgccgagg tccttcatca gcatcttgca ggcgagccga ttgcgaccgt
     3301 tgatccgcat cgcatcggat ccacagatgc cgtgcgcgca ggagcggcgg tacgtgagcg
     3361 tcccgtcctg gtgccacttg acctggttca gcgcgtccag cacgcgatcc agcggctcca
     3421 cttcgagctg gtattcctcg aagtgcgcct tgacatcctg ctccggattg aaccgcttga
     3481 tccgaagtgt gacctccatt ggtcgatcct tccctggtgc tcgagaacgt caatccagtg
     3541 ccgtatcgtg ttagtacttg cgctctttcg gctcgaactt cgtgatcacg atcggcttct
     3601 tcttcagttc gaggccgccg gatgtctggt agatcatgct gtgggcgagc cagttctggt
     3661 cgtcacgcgc ctggtagtcc tcgcgatagt gagcgccacg gctttcggtg cgttcgatcg
     3721 cgccgtggat gatcgtctcg gcgaggtcga gcatgttgcc gagctcgagc gtctccagca
     3781 aatctgtgtt gtagcgcttg ccgtggtcct ggatcgcaac cgcgttgtag gcggacttga
     3841 gctcccggat cttgctcatc gcctcccgca tcccgttctc ctcgcggacg acggagacct
     3901 tgtccatcat cacttcctgc atctcggcgc ggatgtcggc ggcgttctca cccttggtcc
     3961 ggtcgaagag cgaggcgatc tgggatttcg ccatgtattc cggatcgtcc ggaagcatcg
     4021 cgaagtctgt ctcctggatc tcctgcaaca tcgcgcggcc ggcgcggcgt ccgaagacaa
     4081 cgaggtcgac gagcgagttc gtgccgaggc gattcgcgcc atgaacggag acgcaggcgg
     4141 actcgccggc cgaatagaag ccgggaacga cctgcccatc ggggctgaag atcacctggg
     4201 cgttgatatt cgttggcaga ccacccatcg catagtgcgc cgttggctgg atcgggatgc
     4261 cttccttctt cggttcgacg ccgagataga cgcgcgcgaa gtccgtgacg tcggggatct
     4321 tctcgtcgat cacctccggc ggaagatggg aaacatcgag gaagacataa tccttgccgt
     4381 cgatccctcg tccctccttc acttcctggt agatgaagcg ggacatcatg tcgcgaggtg
     4441 cgagatcctt gagcgtcggc gcgtagcggg cggcgaatgc ctcgccgagg ccattgatga
     4501 ccttgccccc ttcaccacgg gcagcctcgg agacgaggat gccgagcttg tagagccccg
     4561 ttggatggaa ctggtacatc tccatatcca tcagggggat gccgcggcga taggcgaggg
     4621 cgattccgtc gccggtgccc gcgatcgcgt tcgaggtgat cttgaagacg cgtccgtagc
     4681 cgccgctcgc gatgagcact gtcttcgcat ggatcgtgtg gatctcgccg gtcttgatct
     4741 cgtaggtgac gacaccgcag caacgaccgt catccgtgaa caggatgtcg actaggtggt
     4801 gctcgtcgaa gaagttcacg ttgtgcttga taccttgctg atagagcgtc tggatgatca
     4861 tgtggccggt ccggtcggcc gcgtagcagg cgcgccgtac gggagcctcg ccgtagttcc
     4921 gggtatggcc gccgaagaat cgctggtcga tcttgccatc ctcggtccgg ttgaagggaa
     4981 ggcccatgtg ctccagctcg agcacggcgt cgacggcctc gcgggcgagc acttccgccg
     5041 cgtcctggtc gacgaggtag tcgccgccct tgaccgtgtc gtacatgtgc cattcccagt
     5101 ggtcttcttc ccggtttccc agcgaggcac tgattccacc ctgggcggca ccggtgtggg
     5161 accgtggggg atagagcttg ctcacgacgg cgatgttggc tttgccggcg agttgaatgg
     5221 cggccatgag accggcgcca cccgccccca ctatgaccgc gtcataccgg tggtaggcca
     5281 tcagatgctc ctttggacgg tgcctgttac gagcgccccg ttgcgctctc cggatctcgt
     5341 ggctggacgc gggtgtagcg ccttggcgcg cccggacaca tcccacttgt gaatcgttcc
     5401 cattttcgca ggaaactccg gatcgtgcct cttcgccgcc gaaccaatcc cgatgacgat
     5461 ctgtgcgcca tcgccagaaa ccgaatgtct gttcggtata cttcttcaaa gttggtgccc
     5521 tgtccgcccg ccttcctggt gtcggcgcgc tcgtcgcccg attgttcgcg cgttaccgcc
     5581 aggagaaccc tttcgtgacc gtccagtccc ccgaactgat tgccaatgtc tcgaagatcg
     5641 ccgaatcgct ggtcgaggag atcgtcgcgc tgacgacgaa gatcacggag atcgccgcgc
     5701 cgacgaacga cgaggccgac cgcgcggcgt tcgtgcacga gctcttcgcg gagcaaggct
     5761 atctcgacgt cgccaccgac ggcctctccg atgtcgtcgg gcgcctgcca ggacgcgact
     5821 cgagcgccgc gccgctgctg cttgcggcgc acaccgacac cgtctttccg cgctcggtgc
     5881 cgatcaaggt cgcccggagt ggcgaccaga tcgcggcccc gggtgtcggc gacaacacgc
     5941 tcagcgtggc cacggtcgcc ctgcttggca aggcgctcaa gcaactcggt gtgacgccgg
     6001 cgacggatgt ctatctgacc ggcaacgttg gcgaggaggg cctcggcgat cttcgcggta
     6061 tgcgcgcggt cgtcgacgcc atccccaacc tcggggcggc gatcgccgtc gaaggacaca
     6121 gccttgggcg aatcacctac cgcgcggtcg gcagcaagcg tctcaaggtg accgtgactg
     6181 gtcccggtgg acatagctgg ggtgacgccg gtcgtccgag tgcgattcac gagatggcga
     6241 agctgatcgc gaagttggac gagattcccc tcaccgcgga gccgaagaca tccttcaacg
     6301 tcgggctgat cgagggcggg tacagcgtca acacgatcgc ggcccaggcg acctgcgtga
     6361 tcgacatgcg atcgaccagc caggagtcgt tggcgaatat cgtcgagaag gtcgagacga
     6421 cgatcgcgcg ggcgtcgaaa aaggaagttg acgtggtgtt cgaggtcgtc ggtgatcgtc
     6481 ccgccggcgc gatgccgctg gattcaggaa tcgtgc
//
LOCUS       DASTMD010000557         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_766129, whole genome shotgun sequence.
ACCESSION   DASTMD010000557 DASTMD010000000
VERSION     DASTMD010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766129"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="VFQ54_08190"
     CDS             <1..1550
                     /locus_tag="VFQ54_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165009.1"
                     /translation="TSSSSTRRVASLAFGTSAGRVPMRYPGSLTSIKQRQTSARGRLS
                     CALLLALLATISLLKPSLAAQSTATGTPTTAATIGGVELVVDGPGAYDANAIAKADTT
                     VIRTAISETATIFGIAPNQVVTIHFVAKADSAATAIMRPIEDLAWASYDGRAALVLRD
                     RFQQLSPAEQENLLRNIISRAAMQTAAGGKLPIGLMDGIARYIERPILADQARAGSLV
                     QAAYQSGSLPDIPTLVNLGAAGLDPETATDSRYALAAFIINRYGVGKLQELVQSFATG
                     APWDTTFQSTLGDATDAMATAWKSFLPRWFSSGWQTNAVAAFDIAPAQALFDRGAYAA
                     ASQQATLSQQLFTELNDQENLSRVETLLALCAVGIQAETLMTETQQALDSHDYVRAQS
                     LLDQANQQYALLPENHRPTQTIDSYESIAQQGLEATRQLDDATSTSHDWLHLRSSRSK
                     ALSAGTTFARLGDDQRAAAARSLLTSVDGRVRRLALILATLAVLLTVWFGVWVRSRAP
                     LRFDWTR"
     gene            1550..>2560
                     /locus_tag="VFQ54_08195"
     CDS             1550..>2560
                     /locus_tag="VFQ54_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165010.1"
                     /translation="MAYSIRTHRFPSRPRVPLEVRAYFTRESLAMQARRARSHAAADP
                     LLFAWQAVTLPLWLGSGIPAPDRPALREPAPDRPALREPALAIEQDLPSAVWLTHELK
                     TVQRRFWLASIASATLRGASIGLIITTLWALMALANLVDLPTATWIFTIAAVGAIAGL
                     GFGLLSKPATLRVAAMLDRTLHLDQRLVTAFDRTTTGERDNPLRQLQLADAANVYAEV
                     KPDIKGTATLSVREGVVFTLAIIALVTVLLLSISSNGMPATAKSVVPQFVPSSQRFAQ
                     EQQQAEAQKQAELSRVAPSTTANSGSDQAATAVQDLNQLANALASNSTTKDAADQIAQ
                     GNY"
BASE COUNT          443 a          943 c          756 g          418 t
ORIGIN      
        1 agacctcatc cagctccacg agacgggtcg cctcccttgc cttcgggacg tccgccggga
       61 gagttcccat gcgatatccc ggatctctca ccagcatcaa gcagcgtcaa acctccgccc
      121 ggggtaggct gtcgtgcgcc ctccttctgg ctctcctcgc gaccatttcg cttctgaaac
      181 catcgctcgc cgcccagtcc acggcaaccg gcacaccaac caccgccgcg acgatcggcg
      241 gcgtcgaact cgtcgtcgat ggacccggtg cctacgacgc caacgcgatc gcgaaggcgg
      301 atacgaccgt gatccggacc gcgatttccg agacggcgac gatcttcggt atcgcaccga
      361 accaggtggt gacgatccac ttcgtcgcca aggcagattc cgccgcgacc gcgatcatgc
      421 gcccgatcga ggatctcgcc tgggcctcct acgacgggcg cgccgcgctg gtcctccgcg
      481 atcgcttcca gcagctctcc ccggccgagc aggaaaacct gcttcgcaac atcatcagcc
      541 gcgccgcgat gcagaccgcc gccggcggca agctaccgat cgggctgatg gacggcatcg
      601 cccgctacat cgagcgaccg attctcgccg atcaggcacg cgccgggtct ctcgtccagg
      661 cggcgtacca gagcggatcg cttccggaca ttcccacgct cgtcaatctc ggggcggcgg
      721 gtctcgatcc ggagaccgcc accgacagcc gctacgcgct cgcggcgttc atcatcaacc
      781 gctacggcgt cggcaagctc caggaactcg tccagtcctt cgcgacgggc gccccgtggg
      841 atacgacgtt ccagtcgaca ctcggcgatg cgaccgacgc gatggcgacg gcatggaagt
      901 cgttcctgcc ccgctggttc tcgtcaggct ggcagacgaa cgccgtcgcc gcattcgata
      961 tcgcacccgc ccaggcgctc ttcgatcgcg gcgcctacgc ggcggcctcc cagcaagcga
     1021 ctctctcgca acagctcttc accgaactga acgatcagga gaacctctcc cgcgtcgaga
     1081 ccctgctcgc gctctgtgct gtcggcatcc aggcagaaac gctgatgacg gagacgcagc
     1141 aggcgctcga ttcgcacgat tacgtgcggg cgcaatcact cctcgatcag gcgaaccagc
     1201 aatatgccct gctccccgag aaccaccggc cgacccagac gatcgactcg tacgaatcga
     1261 tcgcgcaaca gggtctcgaa gccacccgcc aactcgacga tgcgaccagc acctcgcacg
     1321 actggttgca tctccgatcg agtcgttcca aggcgctcag cgcgggcacg acctttgccc
     1381 ggcttggcga tgaccagcgt gcagccgcgg caaggtcgct gctgacgagc gtcgatggcc
     1441 gcgtacgacg gctcgccctg attctggcga cgcttgccgt cctcctcacc gtctggttcg
     1501 gagtctgggt gcgatcacgc gctccgctcc gattcgactg gacacgctga tggcctactc
     1561 gatcagaacg caccgcttcc catcccggcc ccgcgtaccg ctggaggtcc gcgcgtattt
     1621 cacgcgcgag tcgctcgcca tgcaggcgcg gcgagcccgt tcgcacgcgg cagcggatcc
     1681 gcttctcttc gcctggcagg cggtgacgct tccactctgg ctcggctccg gcatcccggc
     1741 ccccgatcga ccagctttgc gggaaccggc ccccgatcga ccagctttgc gggaaccggc
     1801 cctcgccatc gagcaggacc tcccgtctgc ggtctggctc acgcacgagc tcaagaccgt
     1861 gcagcgccgg ttctggctcg cgagcattgc ctcggcaacg ctgcgcgggg cgtcgatcgg
     1921 tctcatcatc acgaccctct gggcgctgat ggcgctcgcc aatctcgtcg acctgccgac
     1981 cgccacctgg atcttcacga tcgccgcggt tggggcgatc gccggtctcg ggtttggcct
     2041 cctctcgaag ccagccacgc tccgtgtcgc ggcgatgctc gaccgcacgc tccatctcga
     2101 tcagcgactc gtcaccgcct tcgatcgcac gaccaccggg gagcgcgaca atccgctgcg
     2161 ccagcttcag ctggcggacg cggcgaatgt ctacgccgag gtcaagccgg atatcaaggg
     2221 aacggcgacg ctgagcgtcc gcgagggagt cgtcttcacg ctcgcgatca tcgcgctggt
     2281 gacagtcctg ctcctcagca tctccagtaa cgggatgccg gcgacggcga agtcggtggt
     2341 cccgcagttc gtaccctcgt cgcaacgctt cgcccaggaa cagcagcagg cagaggcaca
     2401 gaaacaggca gaactctcca gggtcgctcc gagcacgacc gcgaactcgg gctccgatca
     2461 ggcagccacc gcggtccagg atctcaacca gctggcgaac gccctcgcga gcaattcgac
     2521 aacgaaggat gcggcggatc agatcgcgca aggcaactac
//
LOCUS       DASTMD010000558         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2998633, whole genome shotgun sequence.
ACCESSION   DASTMD010000558 DASTMD010000000
VERSION     DASTMD010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2998633"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VFQ54_08200"
     CDS             <1..236
                     /locus_tag="VFQ54_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165011.1"
                     /translation="RVGESIEFEIADDGIGIDEVARNKPGTHGLLGIRERVLAYRGHL
                     EIAGGKHGGTVVRASMPCVAAPEAIPDLAAEVL"
     gene            complement(256..867)
                     /locus_tag="VFQ54_08205"
     CDS             complement(256..867)
                     /locus_tag="VFQ54_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU0165012.1"
                     /translation="MDETTRHPKNAADARQPFLSDIQTLRDRARRHIEQGAIVEGEYR
                     ADARQVCDILNEALATELVCVLRYRRHYYMAKGVLAQPIKAEFLEHSEEEQQHADQIA
                     ERIVQLGGEPNFSPEGLSGRSHAEYVEGTTLVDMIKEDLVAERIAIESYSEIVRFLGD
                     RDPTTRRIMESILAKEEEHADELNDLLEGLPTDNTQGYKLLGA"
     gene            1148..1783
                     /locus_tag="VFQ54_08210"
     CDS             1148..1783
                     /locus_tag="VFQ54_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0165013.1"
                     /translation="MIKKIMLVDDHQIVRAGLKQIFAECTDIVVDAEAASGTEALELL
                     RARPVDGVLLDLSMPDRSGIDVLRRMRGERADLPILILSMHAEEQYAMNVLKAGANGY
                     IAKSSPPSEILKAVRMVLTGKKYVSPALAQQLATAFTSDVHGAPHEKLSTREFEVFCK
                     LAAGHSVSDIADELFLSVKTVSTHRARILEKMNLRTNADLTYYAIKNELIQ"
     gene            2052..2462
                     /locus_tag="VFQ54_08215"
     CDS             2052..2462
                     /locus_tag="VFQ54_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0165014.1"
                     /translation="MNSPLRVFVIEDSPILLERIAEAIADQPSLILVGTADCEKEALV
                     KIKALKPEALVVDIKLREGNGLNVLAQLKWGEGEDAKPKIVVFTNYPRQEYLRNSKQL
                     GADYFLDKSTQFSDLPKVLIGLSYEDRAEQSLRT"
     gene            2481..2642
                     /locus_tag="VFQ54_08220"
     CDS             2481..2642
                     /locus_tag="VFQ54_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEU0165015.1"
                     /translation="MLHYAVVFFVIAIIAAIFGFGGIAAGAAEIAKILFFIFLVLFVV
                     SLLVGLVRR"
     gene            2752..>3031
                     /locus_tag="VFQ54_08225"
     CDS             2752..>3031
                     /locus_tag="VFQ54_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165016.1"
                     /translation="MSGIFHLPRRIGAVAAVIAISMLGNLRLQASESQGDRWSADRAE
                     RLARTADVQGRVFERVGLAEGAVLDADVDDVASDEGPNATGDDNGQFVP"
BASE COUNT          538 a          964 c          985 g          544 t
ORIGIN      
        1 accgcgtcgg cgagtcgatc gagttcgaga tcgccgacga cggcatcggt atcgacgagg
       61 tcgcccgcaa caagccgggc acccacgggc tgctcggcat ccgcgagcgc gtgctcgcct
      121 atcgcgggca cctcgagatc gccggcggga agcacggcgg caccgtggtg cgagcctcga
      181 tgccgtgcgt cgccgcgccg gaagcgatcc ccgatctcgc cgccgaggtc ctgtgacggc
      241 gccgggcccg gccgctcagg cgccgagcag cttgtagccc tgcgtgttgt cggtcggcag
      301 cccctcgagc agatcgttga gctcgtcggc gtgctcctct tccttcgcga ggatgctctc
      361 catgattcgt cgggtcgtcg ggtcgcgatc gccgaggaag cgcacgatct cggagtagct
      421 ctcgatcgcg atccgctcgg cgacgaggtc ttccttgatc atgtcgacga gcgtcgttcc
      481 ttcgacgtat tcggcgtggc tgcggccgct gagcccttcc ggcgagaagt tgggctcgcc
      541 gccgagctgg acgatccgct cggcgatctg gtcggcgtgc tgctgttcct cctccgaatg
      601 ctcgaggaac tccgctttga tcggctgcgc gagcacgccc ttggccatgt aatagtggcg
      661 ccgataccgc agcacgcaga ccagctcggt cgcgagcgcc tcgttgagga tgtcgcagac
      721 ctggcgtgcg tcggcgcggt attcaccttc gacgatggcg ccctgctcaa tgtgtcggcg
      781 ggcgcgatcg cgcagcgtct ggatgtcgga caggaacggc tggcgggcgt cggcggcgtt
      841 cttcggatgt cgggtggtct cgtccatggc gggctctcgg agttcgggcg gcgatcgtac
      901 cgcccgcgcg aggcgcgcgc taggacatcg gacgacagcc gaacgcgccg gccgccgagg
      961 ttgcgaggcg tggccggccg cctaccatcc gcggatcaca tcgtcgtcca cgtgcggtcc
     1021 gacgagacga cgggaacgac cgccgcggcc gctccgcccc atgcctcgat aagccggctt
     1081 cgtgccggct ttttgctgac gcggctacaa tgaaaaaccg aaaagaccgc accgaggacc
     1141 attcccgttg atcaagaaaa tcatgctggt tgacgatcat cagatcgttc gtgccggcct
     1201 caagcaaatc ttcgccgagt gcacggacat cgtcgtcgac gccgaagcgg cgagcggcac
     1261 cgaagcgctg gagctcctgc gcgcgcgacc ggtcgacggc gtcctgctgg atctgtcgat
     1321 gcccgatcgc agcggcatcg acgtgctgcg ccggatgcgc ggcgaacgcg cggacctgcc
     1381 gatcctgatc ctgtcgatgc acgccgaaga gcagtacgcg atgaacgtgc tgaaggccgg
     1441 cgccaacggg tacatcgcca agagcagccc gccgtccgag atcctgaagg ccgtgcggat
     1501 ggtcctgacc ggcaagaaat acgtcagccc cgcactggcg cagcagctgg cgaccgcatt
     1561 cacgagcgac gtccacggcg ccccgcacga gaagctgtcg acccgcgagt tcgaggtgtt
     1621 ctgcaagctc gccgccgggc acagcgtgtc ggacatcgcc gacgaactgt tcctgtcggt
     1681 caagaccgtc agcacgcacc gcgcacggat cctcgagaag atgaacctgc ggaccaacgc
     1741 ggatctcacc tactacgcga tcaagaacga actgatccag tgagtcgtcg ccgctaggga
     1801 aaatgtttgc ggtcgacgcc ctccacccac gcccgcaccc tgcgcacgcc tgccacaaaa
     1861 tggataagac aaacgcggac aggccaacga gcccggaacc gataggggtc gaagacgctg
     1921 gtgactacgc tgggtctcgt gccgattcgt gtgacgcgac gcctacgaat ctgcgaccgg
     1981 attagccccg aatgagatcg atggtcgagg gtggtcggca gggtcccttg aatgccatac
     2041 attgatctcc tatgaattcg cccttgcggg tctttgtgat cgaggattcg ccgatcctcc
     2101 tcgaacggat cgccgaagcg atcgcggatc agccgtcgct gatcctcgtg ggcacggcgg
     2161 actgcgagaa ggaagcgttg gtgaagatca aggccttgaa gcccgaggcc ctggtcgtgg
     2221 acatcaagtt gcgcgaaggc aatggactca acgttctcgc gcaattgaag tggggagagg
     2281 gggaagacgc gaagccgaag atcgtcgtct tcacgaatta cccgaggcag gaatacctgc
     2341 gtaattcgaa gcagttggga gcggattact ttctcgacaa gtcgacgcaa ttttccgatc
     2401 tgccgaaagt gctgatcgga ctgagctacg aggaccgggc ggagcaatcg ctgaggactt
     2461 gaccttaaaa ggagcgtgtc atgctgcatt acgccgtcgt gtttttcgtc attgccatca
     2521 tcgcagccat cttcggcttc ggtgggattg ccgccggtgc agcggaaatc gccaagatcc
     2581 tcttcttcat cttcctcgtg ctgttcgtcg tatctctgct cgtcggactc gtccgacgct
     2641 gaccgagacg aacactgaag accggcgcga tgcgccggtc ttcgcatctg ccgttcgtat
     2701 cgcgcgtctc tgcgcctcgt ccgcgcttca tcaccgacca ggagtcggat catgtcaggc
     2761 atcttccatc ttccgcgacg tatcggcgcg gtggccgcgg tgatcgcgat ctcgatgctc
     2821 ggcaacctgc ggctccaggc gtccgaatcg cagggcgatc gctggtcggc cgatcgtgcc
     2881 gaacgactcg cacggacagc ggacgtgcag ggacgcgtct tcgaacgggt tggcctggcc
     2941 gaaggcgccg ttctcgatgc cgacgtggac gacgtcgcat cggacgaggg cccgaacgcg
     3001 accggagacg acaacggtca gttcgtcccg g
//
LOCUS       DASTMD010000559         1783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1840353, whole genome shotgun sequence.
ACCESSION   DASTMD010000559 DASTMD010000000
VERSION     DASTMD010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840353"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..715
                     /locus_tag="VFQ54_08230"
     CDS             383..715
                     /locus_tag="VFQ54_08230"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEU0165017.1"
                     /translation="MVSSLPIDTQQYLDNVAIVVADEPSSEQLQESDLGDGDEMFGLY
                     TGVPRSERGSGYSMVLPDQITIFMGPLERAFTRRGDLEEQIRITVLHELGHHLGFDED
                     GLDTLGLA"
     gene            712..1776
                     /locus_tag="VFQ54_08235"
     CDS             712..1776
                     /locus_tag="VFQ54_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165018.1"
                     /translation="MNNRNPNAFNSSNRRSVAKPTSGVLGAIANGLSLVLVRPYLIVL
                     PIIVDLVIWLGVQISVKPLTNSLDRFMRDYGGSSGPDAADQISRLGDWLRLNDLIGFF
                     LPSLFGGLPKETSVNWLVLLLAPALADGVDRKSFFDGYGNGPLSTWEPSHSVGVVTLG
                     IGLILISTLLLVLFRMPIARTVRGRKPFHPGMTRELVMTWLSVLGLILLAIFTFVALL
                     IPVTLLVVVSLIFGYGLASLVAIILLSLLGMATIYLIFVLDAILLYHLWPLDAVRLSY
                     RVVKGRFGECLRFALTCLFIQTGLLQVWNVLIENPPGIAISLVGNAFFGAGIVAASMI
                     FFSDRISPADRPLQHHARTS"
BASE COUNT          309 a          555 c          521 g          398 t
ORIGIN      
        1 ccttcatcgg cgatccaacc ccgcgattcg cgggagtcgt cgtgctcgag gagggaagcg
       61 acagcctcgg gacctcggtc caggcatgca ggacgatcct gcaggcgacg atcgagcaag
      121 ccaacgggca gtaagcgaat ccgggcgttg cgcgactgcc gggccgcccg ttcgacgaat
      181 gaaccgtcgg atccctcgcg ggcggttggc ggactccccg aatcgtgcgc aatcggcgaa
      241 agagatcgta cgtacgcggc cttccatacg tactcggact gggattctga ccagaaaaat
      301 ggtaaaataa agcatggctc gtgatccact tcttatggcg cggcgagccc gtcgccaacg
      361 gtttcattca cttgtccaac atgtggtttc gtccttacca atcgacacgc agcaatatct
      421 cgacaatgtc gcgattgtgg tcgcggacga gccgtcaagc gagcaacttc aggaatcgga
      481 tctgggtgac ggggacgaga tgttcggcct ctacacgggt gttccgcgat ccgaacgggg
      541 atcggggtac tcgatggtat tgccagatca gatcacgatt ttcatggggc cgctcgagcg
      601 cgcgttcacc cgacgtggcg atctcgaaga acagattcgc ataaccgtcc ttcacgagct
      661 cgggcatcac ctgggcttcg atgaggatgg tctcgacaca ctaggcctgg cgtgaacaat
      721 cgcaatccga acgcgttcaa ttcgtccaac cggcgatccg tcgccaaacc gacatcgggt
      781 gtgctcgggg ccatcgcgaa tggcctttcg cttgtgctcg tccgtccgta tctgatcgtt
      841 ctcccgatca tcgtggatct cgtgatctgg ctcggtgtcc agatctccgt caagccgctc
      901 accaactcgc tcgatcgctt catgcgggat tacggcggtt ccagcggacc ggacgccgcc
      961 gaccagatct cccgactcgg agactggttg cggctgaacg acctgatcgg attcttcctg
     1021 ccgtcgctct tcggcggcct gccgaaggaa acttcggtga actggctcgt tctcctgctg
     1081 gcgcccgcgc tcgccgacgg ggtcgaccgc aagtcgttct tcgacggcta cggaaacggc
     1141 ccgttgtcga cctgggagcc ctcgcacagc gtcggcgtcg tcacgctcgg gatcggactg
     1201 atcctcatct cgacactgct tctcgtcctc ttccggatgc cgatcgcgcg aaccgttcga
     1261 ggtcggaagc cattccatcc cggtatgacg cgcgaactgg tcatgacctg gctcagtgtg
     1321 ctgggcctga tcctgttggc gatcttcacc tttgtcgcgt tgctgatccc ggtgacgctg
     1381 ctggtcgtgg tctcgttgat cttcggctac gggctggcat cgctggtggc gatcattctc
     1441 ctcagccttc tggggatggc gacgatctat ctcatcttcg tgcttgacgc catcctgctc
     1501 tatcacctct ggcctctcga tgcggtgcgt ctcagctatc gcgtcgtcaa gggtcgattc
     1561 ggtgagtgtc tccgcttcgc gttgacatgt ctgttcatcc agaccggcct cttgcaggtc
     1621 tggaacgtat tgattgaaaa cccgcccggc atcgcgattt cgctcgtggg gaacgccttc
     1681 ttcggagccg gaatcgtcgc tgccagcatg atctttttct cggaccggat ttcaccggcg
     1741 gatcgcccac tccaacatca cgcgcgaacg tcgtgacacg acc
//
LOCUS       DASTMD010000560         1633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2108679, whole genome shotgun sequence.
ACCESSION   DASTMD010000560 DASTMD010000000
VERSION     DASTMD010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108679"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..628
                     /locus_tag="VFQ54_08240"
     CDS             299..628
                     /locus_tag="VFQ54_08240"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEU0165019.1"
                     /translation="MKLILAVVQDEDTDALTESLIESGYRFTKISTTGSFLRTGNTSL
                     MIGVEDAGVDGVMAILQRTCRRRTQIAVPYSPALEPGLLYMPENFEVEVGGAVVFVAN
                     VSRFVRI"
     gene            797..>1633
                     /locus_tag="VFQ54_08245"
     CDS             797..>1633
                     /locus_tag="VFQ54_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0165020.1"
                     /translation="MGSEIREVSPWVERVSREKIVENARQLVSIPSVSGEEAAVMAFV
                     QRWLDERGISYVVTANDPLRPNVIATVGDPTSGPVIAMNGHLDTVPVSDPDTWRTDPF
                     EGVINAEGTRLYGRGAADMKSSVGVMMAMLELFKDAPLTGALQAHIVSDEEIGARFGT
                     LHVLDEIEKGHLPRPDYVFIGEGSEFKIRNAERGGLALEVRFIGRASHTAAARVSGIN
                     AIQKAAKGILALEEHLDKFHPAVGYPVISINTIRGGGAHNVVPGEAIISIDRRLIPGE
                     TKE"
BASE COUNT          295 a          486 c          546 g          306 t
ORIGIN      
        1 gacatccctt cgagctgtat gcgccggaag tcgccgccgc gaatccgatg ctgatcgacc
       61 agattcaccg gctcgtcgcc gcgacgctgg aggcgatgct ctacatcgaa cgcggtggca
      121 acgagctcgg gatcgagtga ccggcccggc ggacattctg gtagactccg ctgaatgctt
      181 gcggacgggg gtgccgcctc cgtggtcgga tcgtgtatga ctcgacatgg atgacggcac
      241 tcccgtattg cctccaggcg aacctgcgtg taggcacacg ttgtaaagga gtgcgccgat
      301 gaagctcatt cttgcggtcg ttcaagatga ggacaccgat gcgctgacag agtcgctgat
      361 cgaatcgggt taccggttta cgaagatcag cacgacgggg tcgtttctgc gaaccgggaa
      421 caccagcctc atgatcggcg tcgaggatgc gggtgtggat ggcgtgatgg cgatcctgca
      481 gcgcacctgc cgccggcgga cccagatcgc ggtgccatac tcgcctgcgc tggagccggg
      541 tctcctctat atgccggaga acttcgaggt cgaagtcggg ggcgcggtcg tcttcgtggc
      601 gaacgtctcg cgcttcgtcc gcatctagga tcctcccccg tctcttcctc gcgcgtcccc
      661 ggccacccga gccggggcgc gatcgtcgtt cttgcgtcaa accggatttc atacgaaggt
      721 tagcgggcga ttaacagcgt cggctggcgt tgccattcca cgcagtgagt tctttacata
      781 tccggaggtt tgcaacgtgg gttccgagat cagggaagtg tcaccgtggg ttgagcgcgt
      841 ctcgcgcgag aagatcgtcg agaacgcgcg gcaactggtc tcgatcccga gtgtgagtgg
      901 cgaggaagcg gccgtgatgg ccttcgtgca gcgttggctg gacgagcgag gcatcagcta
      961 cgtcgtcacg gcgaacgatc cattgcggcc gaatgtgatc gcgaccgtcg gcgatccgac
     1021 gagcgggccg gtgatcgcga tgaacggtca cctcgacacc gtgccggtct ccgatcctga
     1081 tacctggcga accgatccgt tcgaaggcgt catcaacgcc gagggtacgc gcctctacgg
     1141 tcgcggcgcg gcggacatga agagttcggt cggcgtgatg atggcgatgc tggaactctt
     1201 caaggacgcc ccgctgacgg gcgcacttca ggcgcatatc gtctccgacg aggagatcgg
     1261 cgcgcgattc gggacactcc acgttctcga cgagatcgag aaggggcatc tcccgcgtcc
     1321 ggactacgtc ttcatcggtg agggcagcga gttcaagatc cgcaacgccg agcgcggtgg
     1381 tcttgccctc gaggtccggt tcatcgggcg tgcgtcgcat accgccgcgg cgcgcgtgag
     1441 tggcatcaac gcgatccaga aggcggcgaa gggcatcctc gccctcgagg agcatctcga
     1501 caagttccac cctgccgtag gctatccggt gatcagcatc aacacgatcc ggggcggagg
     1561 cgcgcacaac gtggtgccgg gtgaggcaat catcagcatc gatcgtcgcc tgattccggg
     1621 agagaccaag gag
//
LOCUS       DASTMD010000561         1680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_677577, whole genome shotgun sequence.
ACCESSION   DASTMD010000561 DASTMD010000000
VERSION     DASTMD010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677577"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VFQ54_08250"
     CDS             complement(<1..880)
                     /locus_tag="VFQ54_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0165021.1"
                     /translation="MNLEDVAKLAGVSRSTVSRVVNDDPRVSPDVRARVLAVIAETNY
                     HPNAAARSLASRRTRIIGLLIPQAMSEVFGDQWFPVMIQGCIDGCHEADFSLMLLMES
                     STDPETVSRIIERTMRSRHLDGIVISSSLNDEILSARLREEGFPHITIGRDVASLQNF
                     IDVNNREAARMATEHLIGHGYRRIAMISGPDWMVAAEDRRLGFLDAISAAGIEPESVA
                     VRHADYNEFKAYVAATELLSGPCPPEAIFAASDAMARGVIRAAQTLNLRVPEDIAVMG
                     FDGIQQDLIESHGLSTV"
     gene            1211..1552
                     /locus_tag="VFQ54_08255"
     CDS             1211..1552
                     /locus_tag="VFQ54_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEU0165022.1"
                     /translation="MKEAGPGMAWVMLAVAIASEIVATTSLKQSDGFSKPGWSIVVVV
                     GYALSFALMSQTLKTIPVSVAYAIWSGAGTAVIAGIGMVFLGESVSLLKVGGIALIVA
                     GVVALNAGGAH"
BASE COUNT          302 a          493 c          571 g          314 t
ORIGIN      
        1 gcacggtact cagtccgtga ctctcgatca gatcctgctg gatgccgtcg aagcccatga
       61 ccgcgatatc ttcgggaacc cgcaggttca gcgtctgggc ggcccggatc acacctcgcg
      121 ccatcgcatc gcttgcggcg aagatcgctt cgggaggaca cggaccggag agcagctcgg
      181 tcgcggcgac gtaggccttg aattcgttgt agtcggcgtg gcgaaccgcc accgattccg
      241 gctcgatgcc ggcggccgag atcgcgtcga ggaagccgag acggcggtct tccgccgcca
      301 ccatccagtc gggaccggaa atcatcgcga tccggcggta accgtgcccg atcaggtgct
      361 cggtcgccat ccgcgcggct tcgcggttgt tgacgtcgat gaagttctgg aggctggcga
      421 catcgcgacc gatcgtaatg tgcgggaagc cctcttcgcg caggcgggcg ctcaggatct
      481 cgtcgttgag actcgaggag atcacgatac catcgaggtg acgcgagcgc atcgtccgtt
      541 cgatgattcg cgagacggtt tcgggatcgg tcgaggattc catgagaagc atgagactga
      601 agtcggcctc gtggcagcca tcgatgcagc cctggatcat caccgggaac cactgatcgc
      661 cgaagacctc actcatcgcc tgcgggatca gcaggccgat gatccgcgtg cgccgcgatg
      721 cgagggaacg ggcggcggcg ttcgggtgat agttggtctc ggcgatcacg gcgaggacgc
      781 gggctcgcac gtccgggctc acacgcgggt cgtcattgac gacgcgggag accgtggagc
      841 gggaaacgcc ggcgagcttt gcgacatctt cgagattcat cgcgtgccct tgcgaagccg
      901 acacggcttc agtcatgagg gaaccatcgg gatcacgacg atctcgacca atctcccaag
      961 cggaatacgc ctcaagaagc cctgacgaac tgagattgtg ggagcgctcc cacgggaatg
     1021 gcgatagcgt aacccgctct ttctcgaatg tcaacgtcga atccggtgcg gtttctgccg
     1081 tcaatcccac catctggccc attccgatag ccccacggaa tgcctgtcga ttcgcgaggg
     1141 gatcctccac cggagtcgcc gtggaaccgg ctatgctgga attcgagatc gactggagga
     1201 gattcgcagg atgaaagagg cgggaccagg aatggcgtgg gtgatgctgg cggtggcgat
     1261 cgcgtccgag atcgtggcga cgacctcgct gaaacagtcg gacggattca gcaagccggg
     1321 atggtcgatc gtcgtcgtgg tcgggtacgc gctttcgttt gcgttgatga gtcagaccct
     1381 gaagacgatt ccggtgagcg ttgcctatgc gatctggtca ggggcgggga cggcggtgat
     1441 cgcgggaatc gggatggtct ttctcggaga atccgtgagc ctgctgaagg ttggaggcat
     1501 cgcgctgatc gtcgccggtg tcgtcgcgct gaacgccggc ggcgctcact gatcgccgtg
     1561 gtcgtcccgt tgaccgtacc tatggaaaga cgcgttccca tttccgtccc cacggcgctt
     1621 gtcgccgccc cgcgtcgggg acggtgtcgc tgggagggca tgtggcaccg aggcgatccc
//
LOCUS       DASTMD010000562         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2285870, whole genome shotgun sequence.
ACCESSION   DASTMD010000562 DASTMD010000000
VERSION     DASTMD010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2285870"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFQ54_08260"
     CDS             complement(<1..666)
                     /locus_tag="VFQ54_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEU0165023.1"
                     /translation="MIATLVIGLREGLEAALIVGIIAAFLRKNGKSLAPMWIGVTLAV
                     ILSIAVGITLRLVEQALPQSKQEAMESVIGAVAIVFVSGMIVWMNTHSRGMKRDLEEE
                     AAEALSQGGAHSLAVMAFLAVLKEGFETSVFLLATFSAAQSAALAASGAVIGVLIAVV
                     IGWGIYAGGVKLNLSRFFRGTGAFLILVAAGLVITSLRTAHEAGWINAGQQTTVSLSW
                     LVRP"
     gene            1216..2484
                     /locus_tag="VFQ54_08265"
     CDS             1216..2484
                     /locus_tag="VFQ54_08265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEU0165024.1"
                     /translation="MIVQLRDRWLTLIIVANIGFWFANGIHEWFVRGLFQVPGVDWAR
                     FWAASLAFRHAGSAAAYKLPAIAHYMQPILEYYRPEDARVRVGPAPYPPIFLRLFEFL
                     TLPPAPIGYLIWTGLSIGLAAHVFHRLGDRFPTTCRWKFTLLMLSAFPLMMAFYVGQV
                     VALLLVCVMQAMLDFERGRDFRAGMWIGLMILKPQYAVCIMLVLLAKRRWSAIGGATV
                     GGAIILGSSLLVGGVGGLVAYARMLVTDYPSYAGSVAIDPRGMIGWRSLVITIFPDFS
                     TTSSLVLVALLSLATVALLPGIWAGRWDPTSPRFARQMVLTFAITLMVAYHSQPHGAA
                     LLLVPCAMLLAQPDASPLARRLLIGSLAITPIVGTISALVMGNLWLVSLMTSGVLIAL
                     IVILVRPNEPLPETRSVQRPRPRGFRPVPG"
     gene            complement(2719..>2912)
                     /locus_tag="VFQ54_08270"
     CDS             complement(2719..>2912)
                     /locus_tag="VFQ54_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165025.1"
                     /translation="ALRLPDDRDYEIRADRATPDAAAWGAPDFARDPDLGSPIPVERS
                     GRELCWTPTANTRYVVTLP"
BASE COUNT          479 a          944 c          940 g          549 t
ORIGIN      
        1 cggtcgcacg agccacgaca ggctcaccgt cgtctgttgc ccggcgttga tccagcctgc
       61 ctcatgggcg gtgcgcagcg aggtgatcac caatcccgcc gcgacgagga tcaggaaggc
      121 tcccgtgcca cggaagaacc gggacaggtt gagcttcacg ccaccggcgt agatgcccca
      181 gccgatgacg acggcgatga gtaccccgat cacggctccg gatgcggcga gggcggcgga
      241 ctgtgccgcc gagaaggtcg ccagcaggaa cacgctggtc tcgaagccct ccttgaggac
      301 ggcgaggaag gccatcacgg ccagcgaatg cgctcccccc tggctcagcg cctcggcggc
      361 ctcctcctcg aggtcccgct tcatcccacg cgagtgcgtg ttcatccaga cgatcatgcc
      421 ggagacgaag acgatcgcga ccgcgccgat gaccgactcc atcgcctcct gcttcgactg
      481 cggaagcgcc tgctcgacca atctcagcgt gatccccacg gcaatcgaga ggatgacggc
      541 cagcgtgacg ccgatccaca tcggcgccag gctcttcccg ttcttgcgga gaaaggccgc
      601 gatgatcccg acgatcagtg ccgcctcgag cccctcgcgg aggccgatca ccaatgtggc
      661 gatcatctga tgaggtctcc ggttgtgtcg tcgttctgcc cgtcattgac gagatgatgt
      721 gcatccgggc gcgatgcgct ctgtcgttct gccaccagtt agctcccctg ttagttatac
      781 ccgactgaga aagtaggata tgcccgggga gtatggattg caatggtcgt ctcggtcaat
      841 ccatcaggtc gtgtcgaaat gcagcaatcg ccgaggaggg tatgccggga gcatccgcct
      901 ggagacggcg accggcacga tcggtccttc cccacccggc atcggacagc actgaaggcg
      961 ctccgacccg atcccgcatg cgtgcccgct ggttctgctg atccccgcgg ggccagacga
     1021 tgcagcacga ttcccggctc gcacgcacgt ccgcgtcgtg ctccaggcgc cgtcgtggcc
     1081 cgccgcgcat ttcacggcca gttcatactc gctgcccacg attgaagaaa atggagccga
     1141 ggcgccgcgt gaatcgcggc gacagttgcc ggcacgccag atcgacactc gatggaacga
     1201 ggagtagacc gacggatgat cgtgcagctt cgggaccgtt ggttgacgct gatcatcgtc
     1261 gccaacatcg gattctggtt cgcgaatggc atccacgagt ggttcgtgcg gggattgttc
     1321 caggttcccg gagtcgactg ggcgcgattc tgggcggcct cgctcgcgtt caggcacgcg
     1381 ggttccgccg ccgcctacaa gctccccgcc atcgcccact acatgcagcc aatccttgaa
     1441 tattaccgac ccgaggatgc gcgcgttcgc gtcgggccgg cgccataccc tcccatcttt
     1501 ctcaggctct tcgagttcct gacgctgcca ccggcaccga tcggttatct gatctggacg
     1561 gggttgagca tcggcctcgc ggcgcacgta tttcaccgcc tcggcgatcg cttcccgacg
     1621 acgtgccgat ggaagttcac gctcctgatg ctgagtgcgt tccccctgat gatggcgttc
     1681 tatgtgggac aggtcgtcgc gcttctcctc gtctgcgtga tgcaggcgat gctcgatttc
     1741 gaacgcggac gcgacttccg ggccgggatg tggatcggcc tcatgatcct gaagccgcaa
     1801 tatgcggtct gcatcatgct cgtactgctg gcgaagcgga ggtggagtgc gatcggcggg
     1861 gccactgttg gtggggcgat catcctcggg agttcgctgc tcgtcggcgg agtcggtggg
     1921 ctggtcgcct acgcgcggat gctggtgacg gactatccga gctacgcggg gagtgtcgcg
     1981 atcgacccgc ggggaatgat tggctggcga agtctcgtca tcacgatctt tcccgatttc
     2041 agtacgacct cgagcctggt cctcgtcgcc ctgctctcgc tcgcgacggt tgcgctgctg
     2101 ccgggaatct gggccggtcg atgggatccg acatcaccac gttttgcccg gcagatggtg
     2161 ctgacattcg cgatcacgtt gatggtcgcc tatcacagcc agccccacgg cgcggcattg
     2221 ctgctcgtgc cgtgcgcgat gttgctcgca cagcccgacg cgtcaccgct cgcccggcga
     2281 ctgttgatcg gatccctcgc aatcacgccg atcgtgggga cgatctcggc attggtgatg
     2341 gggaatctct ggctggtctc gctgatgacg agcggtgtgc tgattgcctt gatcgtgatc
     2401 ctggtccgtc caaacgaacc tcttccggag acgcgatcgg tgcaacggcc gcgaccccgc
     2461 ggcttccggc ccgttccggg atgagccgcg gggcttggaa gaccttcttc cttggggcgc
     2521 gtctctccct ggcgccgggc gatcacaagg atcggcggcg gcgaccggaa cggcgacaag
     2581 cgccgtaggg aggggatctc aacgatccac gtccgttgcg ccgttcgccc agggatcata
     2641 tcgagcgttg ctcgatgggt cagaccagct gacccactat gccaagggcg ttcctccacg
     2701 tgaaggatca gcgcagagtc acggcagagt cacgacataa cgcgtgttcg cggtcggggt
     2761 ccagcacaac tcgcgcccgg atcgctcgac cgggatcggc gagccgagat ccggatcgcg
     2821 tgcgaagtct ggcgcacccc acgcggcggc gtccggcgtc gcgcgatcgg cccggatctc
     2881 gtagtcacgg tcgtccggga ggcggagcgc ga
//
LOCUS       DASTMD010000563         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1125450, whole genome shotgun sequence.
ACCESSION   DASTMD010000563 DASTMD010000000
VERSION     DASTMD010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125450"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VFQ54_08275"
     CDS             complement(<1..909)
                     /locus_tag="VFQ54_08275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0165026.1"
                     /translation="MKFSLFYNFDILPETDERDLLGYVEAQAVAADALGYDGIWLAEH
                     HFQEYGRMPSPLTFLARLSGLTTGLRLGTAVVEAPYYHPLRLAEDAALLDVLSGGRVQ
                     LGLGSGAGNKELEFERFSIPKEEKSARLMEIVEILRQALAEEIVDFAGEFYTHRDLTI
                     SPRPRQGVQSLLWLAASPTTIPYAARHALPILLPRPVPEQRVRDLIAGYRDTLAPGIP
                     GHVAALRFTFVAETREEAFARTHATFMRYAKYDGGVDWDGRTDTAEYRDLCAKLKFVA
                     GTPDDVAAQLEEWIGDLQPDEILCQMF"
     regulatory      complement(995..1096)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1289..2074
                     /locus_tag="VFQ54_08280"
     CDS             1289..2074
                     /locus_tag="VFQ54_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEU0165027.1"
                     /translation="MENAPYHPARQSLPDLPPSIVAPVYSGADRDGDPTTGRLARRLI
                     LSSLLTAAALAIGLAVLLSAVGMRLPFNSSPAPTKVVLPGVLLYQQQRHLSCEFASVH
                     IATTMIGKPISEYDFDDVVGLNPDPHLGYRGDINGEWGNTDYYGVYNEPLHAALATFG
                     IQSNDFYAEGDRSVLTNEIDAGRPTVVWLAMRGNVDSFYQFTDGGDRYQLTQWMHVMV
                     VYGYDDQNVFLSDPGTAVYRTYSWDDFMAMWNVMDGMSLSVYR"
     gene            2286..>2520
                     /locus_tag="VFQ54_08285"
     CDS             2286..>2520
                     /locus_tag="VFQ54_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165028.1"
                     /translation="MSTIASSAPSGTLEATPAYTNRELFRRFFSYYKPHRKLFWLDFS
                     SAVVSGLLELAFPLAVSYFIDTLLPGNDWGLITL"
BASE COUNT          451 a          815 c          746 g          508 t
ORIGIN      
        1 gaacatctgg caaaggatct cgtctggctg gagatcgccg atccactcct cgagttgcgc
       61 cgcaacatcg tccggcgtcc ccgcaacgaa cttcagcttc gcgcagagat cgcgatattc
      121 cgccgtatcg gtgcggccat cccagtcgac accaccgtcg tacttcgcgt atcgcatgaa
      181 ggtggcgtgg gtgcgggcga acgcttcttc ccgtgtctcg gcaacgaacg tgaaacggag
      241 tgcggcgacg tgaccgggga ttcccggcgc gagcgtgtcg cgatagcccg cgatcaggtc
      301 gcgcacacgc tgctccggca ccggacgcgg caggagaatc ggcaatgcat gtcgcgccgc
      361 gtaggggatg gtcgtcgggc tcgcggccag ccagagcagc gactggacgc cctgccgcgg
      421 tcgcggcgag atcgtgaggt cgcggtgcgt atagaattcg ccggcgaaat cgacaatctc
      481 ctcggcaagg gcctggcgaa ggatctcgac gatctccatc agccttgccg atttctcctc
      541 cttgggaatg ctgaaccgct cgaattcgag ctccttgttt ccggcgccgg accccaggcc
      601 gagctggacg cgtcccccgg agagaacgtc cagcaacgcg gcatcctcgg cgagccggag
      661 cgggtgatag tagggcgctt cgacgacggc ggttccgaga cggagcccgg tcgtgagacc
      721 gctgaggcga gcgaggaagg tgagtggcga cggcattcgc ccgtattcct ggaagtgatg
      781 ctccgccagc cagatcccgt catagccgag cgcgtcggcc gccactgcct gcgcctcgac
      841 atacccgagc aggtcgcgct cgtcggtctc cggcaggatg tcgaagttgt agaagagtga
      901 gaacttcatg ggcgagatcg cctttccccg gacagagctt caacggacgc aaaagagccc
      961 gtccacgatg catggagggc tgccgcgtca gatgcgctca tctttcggac caatgtccga
     1021 cggaattggc accaggtgcc gaagcactgg ttgccgggtt tccaagggcc gttccctcca
     1081 ccactcgcga tgagcacgcg tttcttcgat tgttgcctgc gatcatacgg gcttgcccgc
     1141 aggcaggtca atcgtctctt gggcgctaca cgccctcctt ggaggttcct gcaagactgc
     1201 tgagcgcaag gtgacacctt ttggcgtggt ctcacgttct catgtatgag aacgggtatt
     1261 cgttactcgc caatgaggag tggacccgat ggagaacgcg ccgtaccacc ctgccaggca
     1321 atcgctcccc gacttgccgc cgtcgatcgt cgccccggtc tattccggcg ccgaccgcga
     1381 tggcgacccg acgaccggga gactcgcgcg caggctcatc ctctcctcgc tgctcaccgc
     1441 cgccgcattg gcgatcgggc tcgccgtgct tctcagtgcg gtgggcatgc gccttccctt
     1501 caattccagc cccgcgccga cgaaagtcgt ccttccaggc gtcctcctct accaacagca
     1561 gcgtcatctc agctgcgaat tcgcctccgt ccacatcgcg acgacgatga tcgggaagcc
     1621 gatctccgaa tacgacttcg atgacgtggt tggcctcaat cccgatcctc acctcggcta
     1681 ccggggcgat atcaatggcg agtggggaaa caccgattac tacggtgtct acaacgaacc
     1741 gctccatgcc gccctcgcca cgtttggcat ccagtcgaac gacttctacg ccgagggcga
     1801 ccgcagcgtt ctcacgaacg agatcgacgc cggacgcccg acggtggtct ggctcgcgat
     1861 gcgtggcaat gtcgacagct tctaccagtt cacggacggc ggcgaccgct accagttgac
     1921 gcaatggatg catgtcatgg tcgtctatgg atacgacgat cagaacgttt tccttagcga
     1981 tcccggcacc gctgtctatc gaacctattc ctgggatgac ttcatggcga tgtggaacgt
     2041 catggatggc atgagcctga gcgtctaccg ataatcctca tcgccgaacg actatcttcg
     2101 cggccgtcga tacgctagga tcgagtggag gatcctcgag cgcgatcgcg tgcgtctgtc
     2161 ttcctctggt gttttcagag taaatactgt gtaaactgag cagacatctg gtgacgccgg
     2221 aacgtcgatc gctcgtcgcc tttccattgt gctcggacct ctcccaccac cacggagtac
     2281 ctcccttgtc gactatcgca tcgtccgccc cttcggggac gctggaagcg acgcccgcgt
     2341 ataccaatcg cgagttgttc cgccgcttct tttcctatta caagccacac cgcaagctct
     2401 tctggctcga cttctcgagc gcggtcgtct ctggcttgct cgagctggca tttccgctgg
     2461 cggtctccta cttcatcgat acccttcttc ccgggaacga ctgggggctg atcacgctgg
//
LOCUS       DASTMD010000564         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_500652, whole genome shotgun sequence.
ACCESSION   DASTMD010000564 DASTMD010000000
VERSION     DASTMD010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500652"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFQ54_08290"
     CDS             complement(<1..511)
                     /locus_tag="VFQ54_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0165029.1"
                     /translation="MKITGATTWIVGNPWKNWLFVRLDTDQDGLYGIGEGTINGFAKT
                     TEAAVHEMASRFEGFDPFQIETILQRMSRDFYTEGGQIHMNAIAAVEVACWDIIGKAT
                     GRPIYDLIGGRYHESLPTYANGWYAGPRTPESFAERAQLVMDKGYRALKFDPFGAAWR
                     TMSLEDRHLS"
     gene            complement(569..2050)
                     /locus_tag="VFQ54_08295"
     CDS             complement(569..2050)
                     /locus_tag="VFQ54_08295"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU0165030.1"
                     /translation="MRRRLLGARLLVAFIVIALPSLGMVIGNASGQAAPGTASSSPVA
                     SPEASLPAGVKLQLVAQSLVDPVSVRAPDDGTNRLFVVEKVGIIRVIQDGKLLPKPFL
                     DYSFLTQSGSSEQGMYDIAFHPDFVHNGFVYVSYTDYYVSGATTVLEFHVSSDDPNVV
                     DPSYAARPVIAIQRTNIEHNGGTIAFGPDGYLYLGVGFAPTSAGWSANSDNAQDLQSL
                     DGKILRIDVNVQRDANGRAEAAYNVPANPFGGPTTQKYAYRSYTEQDRGTDRPEIWAY
                     GFRNPWSFSFDPKTGDLYIPDVGESTQEEIDVQRAGDPGGENYGWGGMEGDLCKVKDC
                     SAYVAPAYAYVHDDTHCSVTGIAVYRGQAIPDLNGSFIFSDYCGGEISSLTEVNGKWV
                     ANDLGNIKDVATGVQVTGGGSDAAGNVYVTACGCGGPLGNRYGNPSDPSRATGSVWML
                     VPAGPTAGTPVAGVAPVIAGMPTADLKPVVSGIAAVETPISGN"
     gene            2429..>2972
                     /locus_tag="VFQ54_08300"
     CDS             2429..>2972
                     /locus_tag="VFQ54_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165031.1"
                     /translation="MAITIGQTRGGAKRAAWSARSIVAGSSRNRSDDVLGRLPVECVG
                     AFPNALAHGHQMHRGTLTIHAESLDIDSSHEGSFTLYYDEIAAISLVPSPRRGIPAIQ
                     IRYREDSESAEERGFTAAPRGARSRLDGRQRVEAIATLLASAGIPLARDARGAQPRSL
                     ALSWPDARAHSSELMVWSGLA"
BASE COUNT          541 a          976 c          924 g          531 t
ORIGIN      
        1 gcgagagatg gcgatcctcc aagctcatcg ttcgccaggc cgcaccaaag ggatcgaact
       61 tcaacgcgcg atatcccttg tccatcacca gttgcgcacg ttcggcgaat gattccggcg
      121 ttcgcggccc cgcgtaccag ccattcgcat acgtcggcag cgactcgtga tagcgtccgc
      181 cgatcaggtc atagatcgga cgaccggtcg ccttgccgat gatgtcccaa caggcgactt
      241 cgacggcggc gatcgcgttc atgtgaatct gcccgccttc cgtatagaag tcacgggaca
      301 tgcgctggag gatcgtctcg atctggaacg ggtcgaaccc ctcgaagcgt gacgccatct
      361 catgaacggc ggcctcggtc gtcttcgcga aaccgttgat cgtcccttca ccgatcccgt
      421 acaggccgtc ctggtccgta tcgaggcgga cgaagagcca gttcttccat gggtttccga
      481 cgatccaggt cgtcgcgcca gtgatcttca cgtgctcctc ctttggagtg atctcgcccg
      541 ccagccccgt cgccggcgga atggccacct agttcccgct gattggtgtc tccacggctg
      601 cgattccgga aacgaccggc ttcagatccg cggtcggcat ccccgcgatg accggcgcga
      661 cacccgcgac cggtgtcccg gcggtgggcc ccgccgggac gagcatccag actgaccccg
      721 tcgcgcggga cggatccgat ggattgccat accggttgcc gagcgggccc ccgcagccgc
      781 acgccgtcac atagacattc ccggccgcat cgctcccgcc accggtgacc tggacgcctg
      841 tcgcgacgtc cttgatgttc cccaggtcat tcgccaccca cttcccgttg acctccgtca
      901 gcgacgagat ttcaccgccg cagtagtcgg agaagatgaa ggaaccgttc aggtccggaa
      961 tcgcctggcc ccggtacacc gcgatgccgg tgacggagca gtgggtgtcg tcatgcacgt
     1021 atgcgtacgc cggagcgacg tatgccgagc agtccttcac cttgcagagg tcaccctcca
     1081 tgccacccca gccatagttt tcgccaccgg gatcacccgc acgctggaca tcgatctctt
     1141 cctgcgtcga ttctccgacg tcagggatat agagatcgcc ggtcttggga tcgaacgaga
     1201 agctccaggg attgcggaag ccgtaggccc agatttccgg gcggtcggtt ccgcggtcct
     1261 gctcggtata tgaccggtac gcgtatttct gcgtcgttgg tccgccgaat ggattggcgg
     1321 gtacgttgta ggcggcctcg gcccgcccat tcgcatcccg ctggacgttg acatcgatgc
     1381 ggaggatctt gccgtcgaga ctctggaggt cctgggcgtt gtcggaattt gccgaccagc
     1441 cggcggacgt cggagcgaac ccgactccca ggtagagata gccgtccgga ccgaacgcga
     1501 tcgtgccgcc gttgtgttcg atgttggtgc gctggatcgc gatcaccggc cgggcggcat
     1561 acgagggatc gaccacattc ggatcgtcgc tgctcacatg gaactcgagc acggtggtcg
     1621 ccccggaaac gtagtagtcg gtgtacgaaa cgtacacgaa tccgttgtga acgaaatcgg
     1681 gatggaacgc gatgtcgtac atcccctgct cggaggaacc ggattgggtc aggaacgaat
     1741 agtcgagaaa cggcttcggc agcagcttgc cgtcctggat gacgcggatg atgccgacct
     1801 tctcgaccac gaagagacga ttcgtgccgt cgtcgggagc acgaacgctg acgggatcca
     1861 ccagactctg ggcgacgagc tggagtttga ctccagccgg aagactcgcc tccggacttg
     1921 cgacgggcga ggacgacgcc gtgccgggcg ccgcctggcc gctggcattt ccgatcacca
     1981 tcccgaggga cggcagcgca atcacgatga acgcaaccag aagccgcgct ccaagaagtc
     2041 tccgacgcat gatcccctca actccccgtc tgataccgtg ccgcccaacc gtacgaacag
     2101 gacctgtcta caggaagaga tacgtcgcgt tcggcgaacg acgtctgccg caggctccct
     2161 ttatcgaaca gatcggaaca atcgccaagc cctccattcg gatgtgaccc ttgtacgtac
     2221 acccctccac cgctatcatt gccccgttgt cacaggctat ggggcagtcg acattgcgac
     2281 atcggcaaca tcatcgcgaa acgacaggtc gcaatgagcg ccgcggggaa ctggctgggt
     2341 cccgctggct accgacagga gtcgggctca cgcgatgcac ctgacatgcc gggcgggacc
     2401 cggcgcagac agagagggag gggacgccat ggcgatcacg attgggcaga cgaggggcgg
     2461 cgcgaagcgc gccgcctgga gcgcacgatc gatcgtggcg ggcagttcgc ggaatcgctc
     2521 cgacgacgtc cttgggcgcc tgcccgtcga atgcgtcggc gccttcccga acgcgttggc
     2581 tcacggccac cagatgcatc gcggcacgct gacgatccac gccgagtcgc tggacatcga
     2641 ctcgagtcac gagggcagct tcacgctcta ttacgatgag atcgcggcga tctcgctcgt
     2701 cccttcacca cgacgcggga ttccggcgat ccagattcgc tatcgcgagg attccgaatc
     2761 cgccgaggaa cgaggattta ccgccgcgcc gcgaggggct cgatcccggc tcgacggacg
     2821 ccagcgcgtc gaagcgatcg ccacccttct ggcgtcggcg ggcattccgc tcgcacggga
     2881 cgcccgtggc gcccagccac ggagcctcgc actgtcctgg ccggacgcgc gtgcgcattc
     2941 gtccgagttg atggtctggt cggggctggc ga
//
LOCUS       DASTMD010000565         1966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_140871, whole genome shotgun sequence.
ACCESSION   DASTMD010000565 DASTMD010000000
VERSION     DASTMD010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140871"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFQ54_08305"
     CDS             <1..735
                     /locus_tag="VFQ54_08305"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU0165032.1"
                     /translation="GITPSPSEDVFDHGTFRRYSLASTYVDSVIAAGGTPIILPESID
                     DAQSVLDLVDGLVLSGGSDLDPALYGDSTIHPTTYGVDPERDSFDIRVFKLAVEQDKP
                     VLGICRGIQSINVALGGTLIQDIDSEIPENVVHRQQKIGKNNLETSHSVTIVDEVNPL
                     FAAVGTRNMIVNSFHHQSVRDLAPGLVVTAVAEDGVIEGLWHPGMSFGMAVQWHPEML
                     AARHDDHAAIFRALVGASSGVPSTVR"
     gene            complement(824..>1966)
                     /locus_tag="VFQ54_08310"
     CDS             complement(824..>1966)
                     /locus_tag="VFQ54_08310"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEU0165033.1"
                     /translation="FVLDEVDKIGNDWRGDPSSALLEVLDPEQNNSFRDHHLNVSFDL
                     SKVMFIATANLLDTIPAPLRDRMEILNLSGYTDEDKLNIAKRYLVPKQMKANGLKPDQ
                     TEWSDAALMAIIQHYTREAGVRSLEREIGSAARKVATALAEEREIPAVIGAEQIREYL
                     GRPRYFYEELKDRTSQPGVVIGVAVSGAGGDIMFIEATKMPGKGGLTITGQLGDVMRE
                     SATAAFSFVRSRAKDLGLDETFYTNSDIHLHAPAGAVPKDGPSAGVAMTTALISLLSG
                     IPVREDVAMTGEVTLRGQVLPVGGIKEKALGARRAGVTTFILPRRNEIDLDDLPQELK
                     DEMTFVLADTVDDALKASMPESFHELRNRVRTGPAEVVREPVSIAS"
BASE COUNT          353 a          693 c          552 g          368 t
ORIGIN      
        1 ggcattactc cctcaccgtc ggaggatgtc ttcgatcacg gaaccttccg ccgctacagc
       61 ctcgccagca cctatgtcga ctcggttatt gccgccggcg gcacacctat catccttccc
      121 gagagcatcg atgacgccca gtccgtcctc gatctcgtcg acggattggt cctcagcggc
      181 ggcagcgacc tcgatcccgc cctctatggt gattccacga ttcacccgac aacctatggt
      241 gtcgatcccg agcgagactc attcgacatt cgggtattca agctcgccgt cgagcaggac
      301 aagccggttc tcgggatctg ccgtggcatc cagtcgatca atgtggcgct cggtggcacg
      361 ctgatccagg atatcgacag cgagatccct gaaaacgtcg tccaccgcca gcagaagatc
      421 ggcaagaaca acctcgaaac cagccactcc gtgacgatcg tcgacgaagt gaatcccctc
      481 ttcgcggccg tcggcacccg aaacatgatc gtgaactcat tccaccacca gtcggtccgc
      541 gacctcgcgc ccggattggt ggtcaccgcc gtcgccgagg acggcgtcat cgagggactc
      601 tggcaccccg gcatgagctt cggcatggcg gtgcaatggc atccggagat gctcgccgct
      661 cgtcacgacg accatgccgc aatcttccgg gcgctcgttg gcgcttcgtc cggcgtgccg
      721 agcaccgttc gctgaacctg acgaaacgaa tccggacacg aaaaacgggg tggatccaga
      781 tggatccacc ccgttccgca tcaccgacgc ggcctgcctg tcgctagctg gcgatcgaga
      841 ccggttcccg gacgacttcc gccggccccg tcctcacccg attccggagt tcatgaaagg
      901 actccggcat cgacgccttc agcgcatcgt cgaccgtatc cgcgaggacg aaggtcattt
      961 cgtccttcag ctcctgcggc aggtcgtcga ggtcgatctc gttgcggcgc ggcaggatga
     1021 acgtcgtcac ccccgcccgt cgcgccccga gcgccttctc cttgatgcca ccaaccggca
     1081 gcacctggcc gcgcagcgtc acctcaccgg tcatcgccac gtcctcgcgg acgggaatcc
     1141 ccgagagaag cgagatcagc gccgtcgtca tcgcaacgcc ggcggacgga ccgtccttcg
     1201 gcacggctcc cgccggggcg tgaaggtgga tgtcggagtt cgtgtagaac gtctcatcca
     1261 gtcccagatc cttcgcccgc gagcggacga acgagaacgc cgccgtcgcc gactcacgca
     1321 tcacgtcccc gagctgccct gtgatcgtca ggccgccctt accgggcatc ttcgtcgcct
     1381 cgatgaacat gatgtcacca cctgctcccg agaccgcgac gccgatcacc acgccgggct
     1441 gactcgtccg atccttcagc tcctcgtaga agtagcgagg tcgaccgagg tactcgcgaa
     1501 tctgctccgc cccaatcacg gcgggaatct ctcgctcctc ggcgagtgcc gtcgcgacct
     1561 tgcgggccgc ggatccaatc tcacgttcga gactccggac accggcttcg cgcgtgtagt
     1621 gctggatgat cgccatcagc gccgcatcgc tccactcggt ctgatccggc ttgaggccgt
     1681 tcgccttcat ctgcttcggc accaggtagc gcttcgcgat gttcagcttg tcctcatccg
     1741 tgtagccgct caggttgagg atctccatcc tatcccgcag cggagccgga atcgtatcga
     1801 gcaggttcgc cgtcgcgatg aacatcacct tcgagaggtc gaacgagaca tttaggtggt
     1861 gatcgcggaa gctgttgttc tgctccggat ccagcacctc cagcaacgcc gacgacggat
     1921 caccgcgcca gtcgttgccg atcttgtcga cctcatccag cacgaa
//
LOCUS       DASTMD010000566         5311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1840961, whole genome shotgun sequence.
ACCESSION   DASTMD010000566 DASTMD010000000
VERSION     DASTMD010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840961"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2630)
                     /locus_tag="VFQ54_08315"
     CDS             complement(<1..2630)
                     /locus_tag="VFQ54_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU0165034.1"
                     /translation="MTALVLTQLGFPDIATGWGAAHGVAAQSASDQTLPLPEAGPVVV
                     ILKPQSGFRTAGVAASNDITPTAIYGDAIDGFSADLNDKQIQNLADDPDVAYVVPDIQ
                     FHAAAQSPSTAIARTGLNNSSMAQIDGVDNPVNTDIAIIDSGINPNSPDLNVVGGVNC
                     MSGNPVGSGWADDDGHGTHVAGIAAARDNGTGVVGSAPGARLWAVKVLDSSGNGSLTT
                     VLCGVNWVASNASTIRVANMSLTATITNSSTCTTGNDALHTAICAVAQRNVAVVVAAG
                     NSGIDSQTVVPARYSEVISVAAVTDYDGKPGNLVKSRPSKCPNTGDGADDSLAAYSNY
                     GSIADIAAPGSCILSDWDDGTLHYDTGTSMASPLVAGAAGLYKAQNPGASTSAVLSWL
                     QSTASQAKSSSVGYSGNNGGRILYLGDLSASFPTGTVNPSFSGSKASVHTSWGSSNTS
                     GSSRVTDNDSNTSWYTTNSNPTSGAFVIDLGSLRSLTGVKWQWKLPDGADRMLIQTSS
                     DGVTWQTVGTYQDGSQMYTWYGVALGRAGQYVRVSFTNPRNRAVLGYMTEIQIFAATT
                     TTNAGTRDPSFSGSKLAISTSWGSSNGSGSGRAWDNSTNTSWYTVNKNPTTGSFTLDL
                     GRNRQLTGVKWQFKLPDGADSMLVETSLDGDVWQTVGLFTDKGITNTWLGIALGRSGR
                     YVRFSFANPSHVPVLGYVTEVQIWGNAASLAATTSEPTNPSFDGSRLPIVSSWGSANG
                     SGSGRTWDNDLNTSWYTAGATPASGAFVLDLGQVQTLTGVKWMVKQPGGTDSMLVQTS
                     IDGVTWRSIGEYSEGSVLGTWLGVPVDMSAQYVRFNFANPNDEPVLGYITEVQVWGNA
                     SGATPANTGEADPAFDGAILP"
     gene            3362..4261
                     /locus_tag="VFQ54_08320"
     CDS             3362..4261
                     /locus_tag="VFQ54_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0165035.1"
                     /translation="MAVDAGIKDRGADRAFSIINYVILLLFFLAVAYPLIYVVSASFS
                     DPSAVISGRMWLWPVDVTTNGYRAVFENNKVVTGFRNSLFYTVAGTFVSVVLTILAAY
                     PLSRRDLAGRHIIMGLFLFTMLFNGGLIPTYLTVRALGMLNTPLAIIIPTALSVYNMI
                     ITRTFFQTTIPSEMLEAAQLDGCSDFRFVRDMVIPLSGPIIAVNALFYAVAQWNSYFS
                     ALIYLTNQSLFPLQLILREVLVQNQVDMSQIGDVGKIYQRQQLADLLKYSLIVVASVP
                     VLIIYPFVQKHFVRGVMIGSLKG"
     gene            4308..5297
                     /locus_tag="VFQ54_08325"
     CDS             4308..5297
                     /locus_tag="VFQ54_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0165036.1"
                     /translation="MQLTTPAESRIAAQRTIPKKKASRLSAIRVNVQRSWQLYLMLAI
                     PLAWLFIFNYVPLWGIQIAFRDYRPNGGFAGIWDAKWIGWQNFDRFFNSYNFWPILRN
                     TLVLNVYALVAGFPLPIVLALALNYVGQRWFKKSVQMVSYAPYFISTVVMVGLILQLL
                     ATTGMVNQLLGVVGIDPIPFMTKPAYWKSIYVWTGVWQTVGFGSVIYLAALAGVDPSL
                     HEAAIIDGANKVQRIRDIDIPGILPVAAILLILNMGSLLSSGFEKVILMQNPLNKGTS
                     EVIDSYVYNISLAAPIPQFGYATAIGLFKSVIGLVLLLSVNWLAKRMKAESLF"
BASE COUNT          971 a         1732 c         1558 g         1050 t
ORIGIN      
        1 ggcagaatcg caccgtcgaa cgccggatcg gcttcaccgg tgttcgccgg cgtcgcgccc
       61 gatgcgttcc cccagacctg cacctcggtg atgtagccga ggacgggttc gtcgttgggg
      121 ttggcgaaat tgaaccgcac gtattgcgca ctcatgtcga cgggaacgcc aagccacgtg
      181 ccgaggacac tcccctccga gtactcgccg atcgatcgcc atgtcacgcc gtcgatcgac
      241 gtctgcacga gcatggaatc ggtcccaccg ggctgcttca ccatccactt caccccggtc
      301 agcgtctgca cctggccgag atcgaggacg aacgccccgg acgccggggt cgcgccggcc
      361 gtgtaccagc tcgtgttgag atcgttgtcc cacgtccggc ccgagccgct gccattggcg
      421 cttccccagc tcgagacgat cggcaggcgc gatccgtcga acgacgggtt cgtcggctcc
      481 gatgtggtcg cggccagact tgccgcattg ccccagatct ggacctcggt gacgtaccca
      541 agaaccggca cgtgactcgg attcgcgaag ctgaacctga cgtagcgacc tgaccggccc
      601 agggcgattc ccaaccaggt gttcgtgatg cccttgtccg tgaagagacc aacggtctgc
      661 cagacatcgc catcgagcga ggtctcgacg agcatcgagt ccgcgccgtc gggaagcttg
      721 aattgccact tcacgccggt aagctggcgg ttccggccaa gatcgagggt gaacgagccg
      781 gtcgtcggat tcttgttgac cgtgtaccag ctggtgttcg tgctgttgtc ccaggcccgg
      841 cccgagccgc tgccgttcga gcttccccag ctggtgctga tcgccagctt cgacccggag
      901 aagctggggt cgcgcgtccc ggcattcgtc gtcgtcgtgg cggcgaagat ctggatttcc
      961 gtcatgtacc ccaggacggc gcgattgcgc gggttcgtga agctgacgcg cacatactgc
     1021 cccgcgcgac ccagcgcgac accataccag gtgtacatct gcgaaccatc ctggtacgtg
     1081 ccgaccgttt gccaggtcac gccgtcggac gatgtctgga tcagcatgcg atcggcgcca
     1141 tccggcagtt tccactgcca tttcacaccg gtcagggaac gcagcgagcc aagatcgatc
     1201 acgaaggcgc cgctggtcgg gttcgagttg gtcgtgtacc agctggtgtt gctgtcgtta
     1261 tccgtcacgc gggacgagcc gctggtgttg gaacttcccc aactcgtgtg gacggaagcc
     1321 ttcgagccag aaaaggatgg attcacggta ccagtcggga acgaggccga gagatccccg
     1381 agatagagga tccgcccgcc gttgttcccg ctgtatccga ccgacgacga cttcgcctgc
     1441 gaggccgtgc tctgcaacca gctgagaacg gccgacgtgg acgcgcccgg gttctgcgcc
     1501 ttgtagagcc cggcggcgcc ggcgacgagc ggcgacgcca tgctggtccc ggtgtcgtag
     1561 tggagcgtgc cgtcatccca atcggagagg atgcagcttc cgggcgccgc gatatccgct
     1621 atcgagccgt agttgctgta ggcggcgagg gaatcgtcgg ccccatcacc agtgttgggg
     1681 cacttcgacg gccggctctt gacgagattg cccggcttgc cgtcgtaatc cgtcacggcc
     1741 gcgacggaga tcacctcgga atagcgggcg ggaacgaccg tctggctatc gatgcccgag
     1801 ttcccggccg cgacgacgac cgcgacattc cgctgcgcca ccgcgcagat cgcggtatgc
     1861 agggcgtcgt ttcccgtcgt gcaggtcgac gagttcgtga tcgtcgccgt caggctcatg
     1921 ttggcgaccc tgatcgtcga cgcgttggac gcgacccagt tcacgccgca gagcacggtt
     1981 gtcaacgagc cgttgccgga gctgtcgagc accttgacgg cccagagacg cgcgcccggc
     2041 gccgatccca cgacaccggt cccgttgtcg cgggccgcgg cgatgcccgc gacgtgggtg
     2101 ccgtgtccat cgtcgtcggc ccagcccgag ccgacgggat tgccgctcat gcagttgaca
     2161 ccgccgacga cgttgagatc gggcgaattc ggattgatcc cggagtcgat gatcgcgatg
     2221 tccgtgttca ccggattgtc gacgccatcg atctgggcca tcgagctgtt gttgagaccc
     2281 gtgcgcgcga ttgccgtcga tggcgactga gccgcggcgt ggaactggat atccgggacg
     2341 acgtaggcga cgtccggatc gtcggcgaga ttctgaatct gcttgtcgtt caggtccgcc
     2401 gagaagccgt cgatcgcgtc accatagatc gcggtcggcg tgatgtcgtt cgaagcggcg
     2461 acacccgccg tgcgaaaccc cgattgcggt ttgaggatga ccacgaccgg acccgcctcc
     2521 ggcaacggca gcgtctggtc cgacgccgat tgtgccgcga ctccgtgggc agccccccac
     2581 cccgtcgcga tgtcagggaa ccccaactgc gtcaacacca acgcggtcat cagcgagatg
     2641 aatcccgtcg caccgcacac gcgccggaga cgcccgccct ctcccaggct ccagcttcca
     2701 tgacggtgtt tcatttgtac ccccaaagat gtttgcaagc tcatggccgc ttaggccaca
     2761 gaatacctca gggcaccgtt tacgccatgc acaatattgc agtaggggaa acgtggatgc
     2821 ttccgatacc gattgggccc agacgctgcc aaccactcgg gctatgtctt atttcccgtc
     2881 tgggacgttt gaataacttc tcgcaagcaa ttcgtgcatg agcagaatat tccatcgatt
     2941 tatgactcat agacacaatt gtctgaatca tgaaatgcat gatccatagg catgaaacac
     3001 cattcaatcg gacgcttcgc cccggtacgc ggagatcgat tcgaacggat ccacggatgg
     3061 ggaaacgccc ggactccgga agcctcgccg ggtcgagggg acgtcgtcgg gccggcggcg
     3121 ccctccgcct ccgacccgtc cttgacaacg agttttgttc cttcttatga ttcggccacg
     3181 tagcagcact gtatacagaa cacactcgcg gttcgaccgg agtcgtccgc cgtcaccggc
     3241 ggcgttcgga acgcaccgac ggggagacgc cagtttcgta tctcatcgca ttcatgcgcc
     3301 ggcgccatcg cgagcacagt cactacccgc tggcgagacg acacctgaac ggagacagac
     3361 gatggcagta gacgcgggga tcaaggaccg tggcgcggat cgcgctttca gcatcatcaa
     3421 ctacgtcatc ctcctcctct tctttctcgc cgtcgcctat cccctcatct atgtcgtcag
     3481 tgcatcgttc agcgatccga gtgccgtcat ctccggcagg atgtggctat ggccggtgga
     3541 cgtgacgacg aatggctatc gcgcggtctt cgagaacaac aaggtcgtca ccggattccg
     3601 caactcgctc ttctacaccg tcgccggaac cttcgtcagc gtggtcctca cgatcctcgc
     3661 cgcatacccg ctctcccggc gcgacctcgc cggtcggcac atcatcatgg ggctcttcct
     3721 cttcacgatg ctgttcaatg gaggactgat cccgacctac ctcaccgttc gcgccctcgg
     3781 aatgctcaac acgccactcg cgatcatcat tcccaccgcg ctgtcggtct acaacatgat
     3841 catcacgcgg acgttcttcc agaccacgat cccgagcgaa atgctcgagg cggcgcaact
     3901 ggacggctgc agcgacttcc gcttcgtgcg cgacatggtg atccccctct ccggtccgat
     3961 catcgccgtc aacgcgctct tttatgcggt tgcccagtgg aactcgtact tcagcgccct
     4021 catctacctg accaatcaga gcctcttccc gctccagttg atccttcgcg aggtgctcgt
     4081 ccagaaccag gtcgacatgt cgcagattgg cgacgtcgga aagatctacc agcgccagca
     4141 actcgcggat ctgctcaagt attcgttgat cgtcgttgcg agcgtgcccg tcctcatcat
     4201 ctatccgttc gtccagaagc acttcgtccg cggcgtcatg atcggatcgc tgaagggcta
     4261 gccccgaccc cggcacatga atctcgcaac ggacaaagga cgaccccatg cagctgacaa
     4321 cccccgcaga gtcccggatc gcggcccaac ggaccatccc gaagaagaaa gcatcgcgcc
     4381 tgagtgcgat tcgggtgaat gtgcaaagaa gctggcagct ttatctcatg ctggcgatac
     4441 cgctcgcctg gctgttcatc ttcaactacg tgccgctgtg gggaatccag atcgccttcc
     4501 gtgattaccg cccgaacggc ggtttcgccg ggatctggga tgcaaaatgg atcggctggc
     4561 agaatttcga tcgcttcttc aattcctaca acttctggcc gattctccgg aataccctcg
     4621 tcctgaatgt ctatgcactt gtcgcgggct tcccgctccc gatcgtgctc gccctcgcgc
     4681 tcaactatgt cggccagcgt tggttcaaga aatcggtcca gatggtttcc tacgcgccgt
     4741 acttcatttc gaccgtcgtc atggtcggac tcatcctcca gctcctcgcg acgaccggca
     4801 tggtcaatca actgctggga gtggtcggga tcgaccccat ccccttcatg acgaagccgg
     4861 cctactggaa atcgatctac gtctggaccg gcgtctggca gacggtcggc ttcggcagcg
     4921 tcatctacct cgcggcactc gccggtgtcg atccatccct gcatgaggcg gcgatcatcg
     4981 acggcgcgaa caaggtccag cggatccggg atatcgatat ccccggcatc ctgcctgtcg
     5041 ccgcgatcct gctgatcctg aacatgggca gcttgctgag cagcggattc gagaaggtca
     5101 tcctgatgca gaatcccctc aacaagggca catcggaagt tattgacagt tatgtctaca
     5161 acatcagcct cgccgcaccg atcccccagt tcggatatgc caccgcgatc ggactcttca
     5221 agtcggtgat cgggttggtg ctcctgctct cggtgaactg gctggcgaag cggatgaagg
     5281 cagagagcct cttctagagg gagcacgatc c
//
LOCUS       DASTMD010000567         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2017412, whole genome shotgun sequence.
ACCESSION   DASTMD010000567 DASTMD010000000
VERSION     DASTMD010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017412"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(695..>1500)
                     /locus_tag="VFQ54_08330"
     CDS             complement(695..>1500)
                     /locus_tag="VFQ54_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU0165037.1"
                     /translation="KQANIDAALAGIDAAAGTGARLVVLPEVWTYLGADTGNAAAAEL
                     IPGPLTDLLAERARRHGIFLHAGTMLERVEGQRHLHNTAVVFDPNGEIVARYRKIHLF
                     DVDIDTASYRESDTIAPGDEVVTFELDGVTVGLATCYDVRFPELFRILALRGAEIILL
                     PAAFTLTTGKDHWETLIRARAIENGIFMVATAQVGVHPPGLWCYGRSMVVDPWGLVIA
                     QAADRPSVLTTAIELDEVERVRRQIPSLANRKPDRYRWPDDVLVVAGND"
BASE COUNT          275 a          508 c          465 g          252 t
ORIGIN      
        1 cgcgcataca acgcctgccc gaatacgtcc tcccgagtag tcatgaagcg atcacctcgc
       61 cgtttttcag agcagctcgt cgagggatcc gttggcgtcc gcaagcttcc accgcatctc
      121 ccgctcgtcg tcatgctgaa cgaagtgaag gaccgccgcg cgaagcgcat ccgccgaagg
      181 cggacgccag gaccaacctc tggggtgcgg tgctctcttg aaaacggatc ggcgtcgccg
      241 acccgaggcg acgacaagcg tcgcatctac ggttcatcgc acgaccgcga tcggtgggcc
      301 gcgcccaggc aggaatcata tcgagttgcg ctcgatgggc tgcacgagac ggccagccac
      361 gagagcgcag ccgtccctga tgcggtcgtt cgtgcggcgg gacacggagg tcccgcctcc
      421 tggcgtcagg tttcacaggc agcaaaaagg gccgaccaaa gtggtcgacc ctttttgccc
      481 tggacatcac ggctgttcca cgctacgcgc gtgcaacctc caccgtaatg ccctcagatg
      541 tgagactact catgggcatc acctccttta ccctgttcgg ttcggagtat tctgcggcga
      601 tcgaagctct cttcgaccgt ggatacacag taacatgggc tgccagaggc gtcaacgtga
      661 gagaagtatg cgttcccgta ggtctccgac gcgctcaatc gttcccggcg acgacgagaa
      721 catcgtcggg ccagcggtag cgatccggct tgcgattggc cagcgacggg atctgccgcc
      781 gcacgcgctc gacctcgtcg agctcgatcg cggtcgtcag gacgctcggg cgatccgccg
      841 cctgcgcgat aacgagcccc cacggatcga ccaccatcga gcgaccgtag caccacagtc
      901 ccgggggatg aaccccgacc tgggccgtcg caaccatgaa gatcccgttc tcgatcgccc
      961 gcgcccggat cagcgtctcc cagtgatcct tgcccgtcgt cagcgtgaac gccgccggca
     1021 gcaggatgat ctcggcgcca cgcagcgcga ggatccggaa gagttccggg aaccgcacgt
     1081 cgtaacaggt cgcgagtccg accgtcacgc catcgagctc gaaggtcacc acctcgtcac
     1141 ccggtgcgat cgtgtccgat tcgcggtagg aggcggtatc gatgtcgaca tcgaagagat
     1201 ggatcttccg gtagcgcgcg acgatctccc cgttcggatc gaagacgacc gccgtgttgt
     1261 gcaggtgacg ctgcccctcg acacgctcca gcatcgtccc cgcgtgcagg aagatcccgt
     1321 gacgacgcgc ccgctcggcg agcagatccg tcagcgggcc gggaatgagc tcggccgccg
     1381 cggcattgcc agtgtcagcg ccaaggtagg tccagacctc cggcagcacg acaagccgtg
     1441 caccggttcc ggcggcggca tcgatcccgg cgagcgcggc gtcgatgttc gcctgcttgt
//
LOCUS       DASTMD010000568         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_53419, whole genome shotgun sequence.
ACCESSION   DASTMD010000568 DASTMD010000000
VERSION     DASTMD010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53419"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFQ54_08335"
     CDS             <1..718
                     /locus_tag="VFQ54_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0165038.1"
                     /translation="LRQFSRSRSVLVVIGICLLPVIFALIAYFANRDGTNRDIRQIVG
                     SNIYVNFFAATLLPLATLVLSTAAFGDEIEDKTLQYLTMKPIGRLQIVLAKFIAILIV
                     TIPIAWAGLVITWVVGCWGRLRINDDLIWPMFVSAAVGILGFGALFMLVSLFIQRALL
                     VGIFYVFVWETALSRYLPGIRTVSIRHYTQSTFVRMVDDRLFRLDGVAAMSTILITIA
                     GIVIVSLGLATWRLRRLSLE"
     gene            842..1789
                     /locus_tag="VFQ54_08340"
     CDS             842..1789
                     /locus_tag="VFQ54_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0165039.1"
                     /translation="MSATEQAALGYWPEQKRQMGIGVMIPISEGSAFGGTPRFKDIVE
                     IGNTARDAGFDSLWFADHFSFGAGTDEDPFRGVWEVWTLMAAVAAAVPDLQIGTMVAC
                     TGYRNPGVIAKMTEMIDEVSDGRFILGLGAGWHKPEYDQFGLPFDHRVTRFEEAIKII
                     HPLLREGKADFQGEFFQANKAINQPRGPRPHGAPILVGSSGDRMVGLIAQYADAWNTA
                     WHKDTVRATELSKKVDEALEAAGRDPKTLVKTTGCNIAGTGYTSVRGGAFEGDADAKA
                     GLMNEFKELGFRHFIAGLDPVTPQSVEEFGKAIEIFDKA"
     gene            1846..>2623
                     /locus_tag="VFQ54_08345"
     CDS             1846..>2623
                     /locus_tag="VFQ54_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0165040.1"
                     /translation="MSDLAIGVDFGGTKVLAAVVDVKTGKVLGTGKKRTSSSDGPDDL
                     MKRIYTTCVSAMNDAKVDIKDISGIGVGIAGQVDADRGLLVGTANLSRAVVDLPMADR
                     LQDRFGVPAAIRNDVQIAAIGELTFGAGQGVKDFLCVFVGTGIGGAIVTNGQLVKGAA
                     GNAGEIGHTTVQADGRVCGCGGRGHLEAYASRTGITAALLGELRRGRESILRDLLPGV
                     DPAAPGQPAIRAGILAKAVDAKDDVTIEIIESAGDYLGRGL"
BASE COUNT          442 a          788 c          898 g          495 t
ORIGIN      
        1 gttgcgccag ttctcgcgct cgcgatccgt tctcgtcgtt atcggaatct gcctcctgcc
       61 ggtgatcttc gccctgatcg cctatttcgc caaccgtgac ggcacgaacc gcgacatccg
      121 ccagatcgtc ggcagcaaca tctacgtcaa cttcttcgcg gcgacactcc tgccattggc
      181 gacgctcgtg ctctcgacgg cggcattcgg tgatgagatc gaggacaaga cgctccagta
      241 cctcaccatg aagccgatcg gccggctgca gatcgtcctc gcgaagttca tcgcgatcct
      301 gatcgtgacg atccctatcg cctgggccgg tctcgtcatc acctgggttg tcggttgctg
      361 ggggcgcctg cggatcaacg acgatctcat ctggccgatg ttcgtcagtg ccgccgtcgg
      421 catccttggc ttcggcgcgc tcttcatgct cgtgagcctc ttcatccagc gcgcgctgct
      481 tgtcggcatc ttctacgtct tcgtctggga aaccgcgctt tcgcggtacc ttccgggtat
      541 tcggaccgtg agcattcgcc actacacgca atcgaccttc gtgcggatgg tcgacgaccg
      601 gctcttccgc ctcgatggtg tggccgcgat gtcgacgatc ctgatcacga tcgcgggaat
      661 cgtgatcgtg tcgctcggtc tggcgacgtg gcgattgcgg cggctgagcc tcgagtagga
      721 tgtaatggaa gcacgatgca gagtagccgg attcccgtcg gcgggatggt ggcaggttcg
      781 tgcgcgtgcg atcatgcagc gtgacaacgg ttcggtacgc ccttcagcaa cgggagtgaa
      841 cgtgagcgca acagaacagg cggcgttggg atactggccg gagcagaagc ggcagatggg
      901 catcggcgtg atgatcccga tcagcgaggg aagcgccttc ggcgggacgc cgcgtttcaa
      961 ggacatcgtg gagatcggaa acacggcacg cgatgccggc ttcgattccc tctggttcgc
     1021 cgatcacttc tcgttcggcg cgggtacgga tgaggatccg ttccgcggcg tctgggaagt
     1081 ctggacgctg atggccgcgg tcgcggccgc ggtgccggat ctgcagatcg gcacgatggt
     1141 tgcctgcacg gggtatcgca acccgggcgt gatcgcgaag atgacggaga tgatcgacga
     1201 ggtgagcgat ggccgcttca tcctcggtct tggcgccggc tggcacaagc cggagtacga
     1261 tcagttcggt ctcccgttcg atcaccgcgt gactcggttc gaggaagcga tcaagatcat
     1321 tcatccgctg ctccgcgagg gcaaggcgga ttttcagggc gagttcttcc aggcgaacaa
     1381 ggcgatcaac cagccgcgtg ggccgcgccc gcacggcgcg ccgattctcg tcggctcgtc
     1441 cggcgaccgg atggtcggcc tgatcgcgca gtatgccgac gcgtggaaca ccgcctggca
     1501 caaggacacc gtgcgcgcga ccgagttgtc gaagaaggtc gatgaggcgc tcgaggcggc
     1561 tggtcgtgat ccgaagacac tcgtgaagac cactggctgc aatattgccg gaacgggata
     1621 cacgagcgtg cgtggtggag ccttcgaggg cgacgccgat gcgaaggccg ggctgatgaa
     1681 cgagttcaag gagctcgggt tccggcactt catcgcgggg ctcgatccgg tcacgccgca
     1741 gagtgtcgag gaattcggca aggcgatcga gatcttcgac aaggcgtagc cgcgatcgcg
     1801 gcaaacgatg cccgggggca gcatgacccc cggtcggggg aaatgatgtc tgatctggca
     1861 atcggggtcg attttggcgg taccaaggtt ctggcggcgg tcgtcgatgt gaagaccgga
     1921 aaggttctgg ggaccggcaa gaagcgcacg agttcgtccg acggcccgga cgatctgatg
     1981 aagcgcatct acaccacgtg cgtctccgcg atgaacgacg cgaaggtgga tatcaaggat
     2041 atctccggaa tcggcgtcgg tatcgccgga caggtcgacg ccgatcgcgg tctgctcgtc
     2101 ggcacggcaa acctgagccg ggccgtggtc gatctgccga tggctgaccg gctccaggat
     2161 cgcttcggtg tcccggcggc gatccgcaac gatgtccaga tcgcggcgat cggcgagctg
     2221 accttcggcg ccggccaggg cgtcaaggat ttcctctgcg tcttcgtcgg caccggcatc
     2281 ggtggcgcca tcgtcaccaa tggccagctc gtgaagggtg ccgcgggcaa cgcgggcgag
     2341 atcggtcaca cgaccgtgca ggcggacggt cgtgtctgcg gctgcggtgg tcgcggccac
     2401 ctcgaggctt atgcctcccg taccggcatc accgcggcgc tgctcggcga gcttcgccgc
     2461 ggccgcgagt cgatcctgcg cgatctgctg ccgggtgtcg atccggcggc gccggggcag
     2521 ccggcgattc gggcaggcat cctcgcgaag gccgtcgatg cgaaggatga cgtgaccatc
     2581 gagatcatcg agagtgccgg tgactacctg ggtcggggcc tgg
//
LOCUS       DASTMD010000569         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_946594, whole genome shotgun sequence.
ACCESSION   DASTMD010000569 DASTMD010000000
VERSION     DASTMD010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946594"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFQ54_08350"
     CDS             complement(<1..528)
                     /locus_tag="VFQ54_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0165041.1"
                     /translation="MTGTPRRIGIIGGGFAGLTAALRLAQAGHQVTVWNKGNWGGQAA
                     TFEVAGTRLEVFYHHLFQSDTSIAELANELGVGNELMWLPSNVGYFADGRIYPLNGAV
                     DLLKLGCIPFHDRVRLGLVTAYLQRVKDWKKYEKVTAHKWLERALGKRAYDKTLGAQL
                     RAKFGSYYDQVAMVWF"
     gene            complement(592..1545)
                     /gene="secF"
                     /locus_tag="VFQ54_08355"
     CDS             complement(592..1545)
                     /gene="secF"
                     /locus_tag="VFQ54_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU0165042.1"
                     /translation="MNHLAAKRKLWFSISAILLVPCIISLIFTGLKLGIDFTGGSAWQ
                     IQFDRSVDTEKISQVMTDNGFSDVKVQEIGDKSAHQYMIRTSEIQEGSAEKKKLETDL
                     TKLGTLNPKGESLETVGASVGKSIQNRAVLAVAAASVGILLYIAYAFRNTQNPFLYGS
                     AAIVAMLHDAIFVLGVFSIAGWLFDVEVDSLFITALLTIIGFSVHDTIVVFDRIRENL
                     ANKVSSQFEEVVNYSIAQTIVRSVNTSMTVLITLLALYLFGGASTKDFVFALLIGVAA
                     GTYSSIFNASQVLVAWEYHEIQDAFNRIFRRGRTPRPRPAS"
     gene            complement(1582..>2788)
                     /gene="secD"
                     /locus_tag="VFQ54_08360"
     CDS             complement(1582..>2788)
                     /gene="secD"
                     /locus_tag="VFQ54_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU0165043.1"
                     /translation="DQILVELPGVDDPQQAVNVLQQTAQLEIIDPNGDTLAQGQTVKT
                     TLSEQRAASEAASTPSASPSPVATPAASPVGSTPAAVAGVVGPEPLATTPTPTPDTST
                     GPVYTTLVSGTDLKNAYATTDQTGQWVVAFELKSGAAGDFYDYTSQHIGSQIGFVLDG
                     TVISAPVLQSAISSQGQITGMTAQSAHDLALQLKAGALGVPLTVVSSTTVGPSLGQDS
                     IDRSLIAGIIGLSAVAFFMIVYYRIPGVLAVVALLIYTALTFALYRLIPVTLTLPGIA
                     GFILSIGMAVDANVLIFARLKEELRTGRDLNRAIEAGFQHAWPSIRDSNISTMITCAI
                     LYWFGQFTGATIITGFALTLFVGVAVSLFTAITVTRTFLRIMVESGLIRGSWWLGLDR
                     AEIQAIAGD"
BASE COUNT          545 a          796 c          933 g          514 t
ORIGIN      
        1 gaaccagacc atcgcgacct ggtcatagta ggagccgaac ttcgcgcgaa gctgggcgcc
       61 gagcgtcttg tcgtaggcgc gcttgccgag cgcccgctcc agccacttat gcgcggtcac
      121 cttctcgtac ttcttccagt ccttcacgcg ctgcaggtag gccgtcacca ggcccaggcg
      181 gacgcgatcg tggaagggga tgcatccgag cttgagcagg tcgaccgcgc cgttcagcgg
      241 atagatcctg ccgtcggcga aataccccac gttggacgga agccacatca actcgttgcc
      301 gacgccgagc tcgttcgcga gttcggcgat cgacgtgtcg ctctgaaaga ggtggtggta
      361 gaagacctcg agtcgcgtgc cggcgacttc gaaggtggcc gcctgaccgc cccagtttcc
      421 cttgttccag acggtgacct gatgccctgc ctgggcgagc cgcaatgcgg ccgtgagacc
      481 ggcgaatccg ccgccgatga taccgattcg tcgaggggtg ccggtcatgg gcgcgcaggg
      541 ccttcctgtt ctgacggtct ggacgtcagg cgagagtcac gaatggtcga actaggatgc
      601 cgggcgtggg cgcggcgtgc gtccacgtcg gaagatgcga ttgaacgcat cctggatctc
      661 gtgatactcc cacgcgacca gaacctggct cgcattgaag atcgacgaat aggttccggc
      721 ggcgacgccg atcaacaggg cgaagacgaa gtccttggtc gacgcacccc cgaagagata
      781 gagcgcgagc agggtgatga gcacggtcat cgatgtgttg acggaccgga cgatcgtctg
      841 cgcgatcgag tagttcacga cttcctcgaa ctgggacgat accttgttcg cgaggttctc
      901 ccggatccgg tcgaagacga cgatcgtgtc gtggaccgag aatccgatga tcgtgagcaa
      961 ggccgtgatg aagagggaat cgacctcgac atcgaagagc cagccggcga tcgagaagac
     1021 accgagcacg aagatcgcgt catgaagcat cgcgacgatt gcggcggagc cgtataggaa
     1081 gggattctgg gtattccgga aggcgtaggc gatgtacagc aggatgccga cagaggccgc
     1141 agcgaccgcc agcaccgccc ggttctggat cgatttaccg accgaggcac ctaccgtttc
     1201 aagactctcc cccttgggat tgagcgtccc cagcttggtg agatcggtct ccagcttctt
     1261 cttctcggct gagccttcct ggatctcgga ggtgcggatc atgtactggt gcgcgctctt
     1321 gtcaccgatc tcctggacct tgacgtcaga gaagccgttg tcggtcatca cctgcgagat
     1381 cttctcggtg tcgacgctgc ggtcgaactg gatctgccag gccgaaccgc cggtgaagtc
     1441 gatgcccagc ttgaggccgg tgaagatgag cgagatgatg cagggcacaa gcaggatcgc
     1501 cgagatggag aaccagagct ttcgttttgc cgcgagatga ttcatgatcg agaaactccc
     1561 agaatcgcgt tggtgacgac gttagtcccc ggcaattgcc tgaatttcgg cgcggtcgag
     1621 tccgagccac caactgccgc gaatcaggcc gctttcgacc atgatgcgga ggaacgtgcg
     1681 ggtgacggtg atcgccgtga agagactgac cgcgactccg acgaagagcg taagtgcgaa
     1741 tccggtgatg atcgtcgcgc cggtgaactg gccgaaccag tagaggatcg cgcaggtgat
     1801 catcgtcgag atgttggaat cacggatcga cggccaggca tgctggaatc ctgcctcgat
     1861 cgcccggttc aggtcgcgac cggtgcgtaa ttcctctttc agtcgcgcaa agataaggac
     1921 attcgcatcg acggccatac cgatcgagag aatgaatccg gcgatacccg gcaatgtgag
     1981 cgtgactgga atcaacctgt agagggcaaa ggtaagcgcc gtgtagatga gaagggcgac
     2041 cacggcgagg acgccaggga tgcggtagta gacgatcatg aagaacgcca cggccgaaag
     2101 cccgatgatg cctgcgatga ggctgcggtc gatcgaatcc tgaccgaggg atggccccac
     2161 agtggtgctg gagacgaccg tcagcggtac gccgagagcg ccggccttga gctgaagcgc
     2221 caggtcgtgc gcgctctgcg ccgtcatccc ggtgatctgg ccctggctgg agatcgcact
     2281 ttgcaggacc ggagccgaaa tcacggtgcc gtcgaggacg aagccgatct ggctaccgat
     2341 atgctggctt gtgtagtcgt agaaatcgcc ggcggccccg ctcttcaact cgaacgccac
     2401 gacccactgg ccggtctggt cggtggtcgc gtaggcgttc ttgagatcgg ttccgctgac
     2461 gagggttgtg tagacgggac cggtgctcgt atcgggagtc ggcgtgggtg tcgtcgccag
     2521 cggttccgga ccaacgacgc cggcgaccgc ggccggagtc gagccaacgg gactggccgc
     2581 gggtgtggcg accggtgacg gcgaagccga tggggtggag gccgcctcag atgcggcgcg
     2641 ttgctcgctg agggtcgtct tcacggtctg gccctgcgca agcgtatcgc cgttgggatc
     2701 gatgatctcc agttgcgccg tttgctggag aacgttgacc gcctgctggg gatcgtcgac
     2761 tcccggcaac tcgacaagga tctggtcg
//
LOCUS       DASTMD010000570         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2732158, whole genome shotgun sequence.
ACCESSION   DASTMD010000570 DASTMD010000000
VERSION     DASTMD010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2732158"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFQ54_08365"
     CDS             complement(<1..632)
                     /locus_tag="VFQ54_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165044.1"
                     /translation="MPADSAAAILALVRAIDRHISSALPSPADDLVFGKALANPGGNR
                     ALQLCLDASGQQVADGSVGLQQWAAEFLDACNDLVAAEQVLAHIESGFMRAVGEPGES
                     LDAWIATKRVPTSWRERADFASWASVAAASTTDVVDAMAYQLGYPPETEIDGCSVGTY
                     VDVLRALIDLAPRSWNEGEDAPFHSEDSLVSGIASGLGLEPETARRAISA"
     gene            complement(798..1775)
                     /gene="galE"
                     /locus_tag="VFQ54_08370"
     CDS             complement(798..1775)
                     /gene="galE"
                     /locus_tag="VFQ54_08370"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU0165045.1"
                     /translation="MRVLVTGGAGYIGSNATERLLLRGHDVIVADNFERGHRSAVHPD
                     AEVLELDLRDGDSIVRSLRGRQIEAVLHFAALHLVPESVQKPSEYYRTNVVGGFNLLD
                     AVRELGIGKFVFSSTAAVYGEPEELPIVETSPKRPINPYGKSKWMIEQMLEDFSERYG
                     ISYAAFRYFNVAGASGQFGEDHRPETHIIPVALQALQGKRDAFTIFGTDYATNDGTAV
                     RDYVHVVDLAEAHATVLDHLDTSLGAINLGTKDGFSVQQIATAVELTTDQELPLAYGG
                     RRAGDPAALVADSTKAQELIGWNPARSTLDEMIGSAWDWMQEHPDGYPD"
     gene            1964..>2396
                     /gene="glyA"
                     /locus_tag="VFQ54_08375"
     CDS             1964..>2396
                     /gene="glyA"
                     /locus_tag="VFQ54_08375"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU0165046.1"
                     /translation="MFTPDLLAQADPEVASAIAKEERRQREGIELIASENYVSEAVLA
                     AVGSVLTNKYAEGLPGKRYYGGCEYVDIVEELANARARELFGVEHVNAQPHSGANANL
                     AAYFSLLNPGDRILGLNLSEGGHLTHGRDVNFSGRFLEPHFY"
BASE COUNT          449 a          774 c          758 g          415 t
ORIGIN      
        1 aacgccgaga tggcccggcg tgcggtttcg ggctcgagtc caaggccgga ggcaatcccc
       61 gagaccagcg aatcctcgga gtggaagggg gcgtcttccc cctcattcca ggatcgaggc
      121 gccaggtcga tcagcgcccg caggacatca acgtacgtcc ccacagaaca gccatcgatt
      181 tccgtttccg ggggatagcc cagttggtag gccatcgcat cgacgacatc ggtcgtgctg
      241 gcggcagcga ctgatgccca cgaggcgaaa tcggcgcgtt cgcgccagga cgtcggaacg
      301 cgcttcgtcg cgatccaggc atcaaggctt tcgccgggct cgcccacagc ccgcatgaac
      361 ccggactcga tgtgggcgag cacttgctcc gccgcaacaa ggtcgttgca tgcgtcgaga
      421 aattcggctg cccattgctg gagaccgact gatccatcgg ccacctgctg tcccgacgcg
      481 tcgagacaga gttgcagggc gcgattcccg cctggattcg cgagcgcctt gccaaacacg
      541 agatcgtcgg caggggaggg gagcgcgctc gagatgtgcc ggtcgatcgc gcggaccagc
      601 gcgaggatgg ccgccgcact atcggccggc acaccctcac gcaatcgttc gttgcgttgg
      661 tctatcgcct ggcgaaggcg ctccggtgcg gtcatggttg gggccggtcg catctgccca
      721 agggaaaggc tgcaatcagg tctccgggtc tcgatgaccg ttaccatgcc acggcaatcg
      781 ccgatcgaca accggagcta gtccggatag ccgtccggat gttcctgcat ccagtcccac
      841 gcggagccga tcatctcatc gagcgtcgag cgcgcgggat tccagccgat cagttcctgg
      901 gccttggtcg aatccgccac aagcgcagcg ggatcgccgg cgcgccgtcc accataggcg
      961 agtgggagct cctgatcggt cgtcaactcg acagcggtcg cgatctgctg gacggagaat
     1021 ccatccttcg tcccgaggtt gatcgcaccg agggacgtgt cgaggtgatc gaggacggtc
     1081 gcatgcgcct cggcgagatc gacgacgtgg acgtagtcac ggaccgccgt cccgtcgttc
     1141 gtggcgtagt cggttccgaa gatcgtgaag gcgtcgcgct tgccctggag ggcctggagg
     1201 gcgacgggaa tgatgtgcgt ctccgggcga tgatcctcgc cgaactgacc gctcgccccc
     1261 gcgacgttga agtaccggaa ggcggcgtag gagatgccat atcgttccga aaaatcctcg
     1321 agcatctgct cgatcatcca cttggacttt ccatagggat tgatcggtcg cttcggcgat
     1381 gtctccacga tcggaagttc ttccggttcg ccgtaaacgg cggccgttga cgagaagacg
     1441 aacttgccga tcccgagttc gcgcacggca tcgaggaggt tgaaacctcc gacgacattc
     1501 gtccgatagt actcgctcgg cttctgcacc gactccggca ccagatggag cgccgcgaag
     1561 tgaagcaccg cctcgatctg gcgtccgcga agcgagcgga cgatcgagtc gccgtcgcga
     1621 agatcgagct cgaggacctc ggcatcggga tgcaccgcgg accgatgccc ccgctcgaag
     1681 ttgtcggcga caatcacgtc gtgacctcgc agcaacaacc gttccgtggc attgctcccg
     1741 atgtagccgg cgccaccggt gacgagaacg cgcatgtatg ctccttgaat gatcgatgtc
     1801 gtggtcagca gagaaccccg gcacgaggcc ggggtcaggc tcccatcatg gtacactctt
     1861 gcccgatgag cgatgcgctg ccagcgtatc caacgcgcga tcctgccgcg cagttcccct
     1921 gagatcattc ggggtacgtc tcgcccgaag gagcccaaac cagatgttca ccccagacct
     1981 gctggcccag gccgaccccg aagtcgcctc ggctattgcc aaggaagaac gccgccagcg
     2041 cgagggaatc gagctgattg cctcggaaaa ctacgtcagc gaagcggtac tcgcggcggt
     2101 cggcagcgtg ctcacgaaca agtatgccga ggggctcccg ggcaagcgct attacggtgg
     2161 ctgcgaatac gtcgatatcg tcgaggaact cgcgaacgcc cgcgccaggg aactctttgg
     2221 cgtcgagcac gtgaacgcgc agccgcactc gggtgcgaac gcgaatctcg ccgcgtactt
     2281 ctcgctcctg aatcccggcg acaggatcct cggcctcaac ctctccgaag gcggccacct
     2341 gacgcacggg cgggacgtca acttctccgg gcgcttcctc gaaccccatt tctacg
//
LOCUS       DASTMD010000571         3816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1571734, whole genome shotgun sequence.
ACCESSION   DASTMD010000571 DASTMD010000000
VERSION     DASTMD010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571734"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFQ54_08380"
     CDS             <1..228
                     /locus_tag="VFQ54_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165047.1"
                     /translation="GALRIVLLRVLVEETEISKLVTNITRIVTVAAQAMRIQHAVSGE
                     GTTSMLESVNAMLQSVLEDPLPVLDETERKP"
     gene            225..524
                     /locus_tag="VFQ54_08385"
     CDS             225..524
                     /locus_tag="VFQ54_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165048.1"
                     /translation="MTTFTDAQRERRSTATTNLRVNLSRLGNLPDVSSPPTPEDREPG
                     IDLKPTSAYEAVTRQKVDSLADDLREIKSRLNNLVFVIAGGILLDVIGRMLGIGT"
     gene            521..2002
                     /locus_tag="VFQ54_08390"
     CDS             521..2002
                     /locus_tag="VFQ54_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165049.1"
                     /translation="MSESLPRERLRRIVTDVAAFSQALLPRQRLRPYQIEPARAIAES
                     VIQRRGDQFAVVFSRQAGKDEMLAQLLAMLLLRFSQRGGSIVVAAPTYRPQAALSRDR
                     LRDRLQANPLTAPLVTVRDGYALGVGRATARFLSAAPGANARGQTADLLLVANEAQDI
                     DPDTWDAVFDPMAASTNATTVFMGTVWSASTLLARQMRHLARMEDEDGRQRIFRVPWD
                     TVAAEIPAYGDRVRARIAQLGETHPFVRTKYFLQELDGESSLFPPHRLAQMQGDHPRQ
                     HRATPGCRYALLLDVAGEEEAGGDQLAFSPGNARRDSTALTVVAVESAERTDGRASYR
                     VVDRRIWTGIRHTALHDQLVDLARNVWKASWVVVDATGIGAGLASFLQASLGRRSGSG
                     DASLRVVPFAFTSASKSKLGWDFLGLIDSGRFKEYADDREEATRLYQTQLSATTFEIM
                     NGPGNILRWSVPERDGHDDLVISAALCAVLDDLDPRPRVAVGS"
     gene            complement(2071..3750)
                     /locus_tag="VFQ54_08395"
     CDS             complement(2071..3750)
                     /locus_tag="VFQ54_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0165050.1"
                     /translation="MSTLAELMSRRKLVGAATAAGVTIAAGHALASAQATPDATPASQ
                     EAQLTIDQTAAQPTRLGAAVPPEFTSNETNWPTEHGNLSGTRSAAGSSISLKTIDTLG
                     VAWRIPVEAPGTYGSLTSTPIVVDDTIYLIDMQSNIWSIDKATGKTNWKTEYNVGLLG
                     PNGLAVAYGQVFGALGDTSEVVSVDGKTGKENWRVQLSNNKYEGIDIAPFVYDSIVYV
                     STIPGNSTSFYQGGSKGILYGLDASTGLTLFQWDTTGDGLWGNFRVNSGGGLWHPPVV
                     DDNGYLYFSVANPAPWPGNKEFPNGTSRPGDNAYSNSLVSMDPDGGFIRWHIGVKPHD
                     LFDLDLHLSPILAKVTIDKKERNVVFASGKLGIVIAADTMTGEELWRVPVGTHKNDNL
                     QELGTDYVEIYPGNNGGVETPMAYADGIIYCAVLEMPSSYNASGYADPLFDLNTATGL
                     LVALDATSGNVVWQAKLPTAAYGSAVVANDVVFTGGLDGVVLGFNAKTGEQVFSFQTG
                     SGLNAPASISGDYLFIPATGPLIPSSATKDPKPAQELIALKIGSGAATPTS"
BASE COUNT          644 a         1201 c         1285 g          686 t
ORIGIN      
        1 ggcgcgctgc gcatcgtgct cctccgcgtc ctcgtcgagg agaccgaaat ctcgaagctc
       61 gtcaccaaca tcacgcggat cgtgacggtg gcggcccagg cgatgcggat ccagcacgcc
      121 gtctccggcg aggggacgac atcgatgctg gaatcggtca acgcgatgct gcagagcgtg
      181 ctcgaggatc cgctgccagt cctggatgaa acggagcgaa aaccatgacc accttcactg
      241 acgcccagcg ggagcgacgt tccacggcaa cgaccaatct ccgcgtgaat ctctcgcggc
      301 ttggcaatct cccggatgtc tcgtccccgc caacgccgga ggatcgcgaa cccgggatcg
      361 acctcaagcc gaccagcgcc tacgaggccg tcacccggca gaaggtcgat tccctggcgg
      421 acgacctgcg ggagatcaag tcacgcctga acaacctggt cttcgtgatc gccggcggga
      481 tcctgctcga cgtgatcggg aggatgctgg ggatcggcac gtgagcgaat cgctcccgcg
      541 cgaacgcctg cgacggatcg tcaccgatgt tgccgccttc tcgcaggcgc ttctgccccg
      601 acagcgcctc cgtccctacc agatcgaacc ggcgcgggcg atcgccgaga gcgtgatcca
      661 gcgacgtggc gatcagttcg cggtcgtctt cagccggcag gcagggaagg acgagatgct
      721 tgcccagctc cttgcgatgc tcctgctccg cttctcccaa cggggaggat cgatcgtcgt
      781 cgccgcgccg acgtatcgac cgcaggcggc gctcagccgg gatcggctgc gcgatcgcct
      841 ccaggcgaac ccgctcaccg cgccgctggt gacggtgcgg gatggctacg cgctcggcgt
      901 cggccgggcg acggcacgct tcctctccgc cgcccctggc gccaatgctc gcgggcagac
      961 ggccgatctt ctcctcgtcg cgaacgaggc gcaggatatc gatcccgaca cgtgggatgc
     1021 cgtcttcgat ccgatggcgg catcgaccaa tgccacgacc gtcttcatgg gaacggtctg
     1081 gagcgcctcg acgctgctcg cgcggcagat gcgtcacctc gcgcggatgg aggatgagga
     1141 tgggcgacag cgcatctttc gggtgccgtg ggacacggtc gccgcggaga tccccgcgta
     1201 tggcgatcgc gtccgcgcgc gcatcgccca actgggagag acgcatccct tcgtccgcac
     1261 gaagtatttc ctgcaggagc tcgacggcga gagcagcctc tttccgccgc accggctggc
     1321 acagatgcag ggcgatcatc cccggcagca ccgggcaacc ccgggatgtc gctacgcgct
     1381 gctgctcgat gtcgcggggg aagaggaggc cggtggcgac cagctcgcct tcagccccgg
     1441 aaacgcccgg cgggacagca cggcactcac cgtcgtcgcc gtcgaatcgg cggaacgtac
     1501 cgatggccgg gcatcgtacc gggtcgtcga tcgtcgcatc tggacgggca ttcgccacac
     1561 ggcgctccac gaccagctcg tcgatctggc gcggaacgtc tggaaggcaa gctgggtcgt
     1621 cgtcgatgcg acgggaatcg gcgcgggatt ggcatcgttc ctccaggcgt cgctcggccg
     1681 gcgatcgggt tccggggatg cgtccctgcg tgtcgttccc ttcgcattca cgagcgcgag
     1741 caagagcaag ctcggctggg atttcctcgg actgatcgac agcggccgct tcaaggagta
     1801 tgcggacgat cgtgaggagg cgacgcgcct gtaccagacc caactttcgg ccacgacgtt
     1861 cgagatcatg aacggcccgg ggaatatcct gcgctggtcg gtccccgagc gagacgggca
     1921 cgacgatctg gtgatcagcg ccgccctgtg cgcggtactc gacgatctcg atccacgacc
     1981 gcgggtcgcg gtcggctcat gaatgacgca tccccggcgg acgatgtcca ccggggatgt
     2041 gtcatgtcta tcgcgggacg agggatcccg ttagctggtt ggcgtcgccg cgccagagcc
     2101 gatcttgagt gcgatcaact cctgggccgg cttcggatcc ttcgtcgcgg acgacgggat
     2161 gaggggtccg gtcgccggaa tgaagagata gtcgccggag atcgatgcag gcgcgttcag
     2221 accggatccg gtctggaaac tgaagacctg ctcgcccgtc ttcgcgttga agccgagaac
     2281 gacaccgtcg aggccgccgg tgaagacgac atcgttcgcc accaccgcgc tgccgtacgc
     2341 ggccgttggc agcttcgcct gccagacgac gttgccgctc gtcgcgtcga gggcaacgag
     2401 gagtccggtg gccgtgttga ggtcgaagag cggatccgca taaccactgg cgttgtagct
     2461 gcttggcatc tcgagcacgg cgcaatagat gatgccgtcg gcataggcca tcggagtctc
     2521 gacaccaccg ttgttgccgg ggtagatctc gacgtagtcg gttcccagtt cctggaggtt
     2581 gtcgttcttg tgggtgccca ccggcacgcg ccagagctcc tcgccggtca tcgtgtcggc
     2641 ggcgatgaca atacccagct tgccggaggc aaacacgacg ttgcgctcct tcttgtcgat
     2701 cgtcaccttg gcgaggatcg gcgagagatg gaggtcaagg tcgaagagat cgtgaggctt
     2761 gacgccgatg tgccagcgga tgaagccgcc atccggatcc atcgagacga gcgagttgga
     2821 gtaggcgttg tctcccgggc ggctggtgcc gttcgggaat tccttgttcc ccggccacgg
     2881 tgccgggttc gcgacggaga agtagagata gccgttgtca tcgacgaccg gcgggtgcca
     2941 gagaccgccg ccggagttca cccggaagtt tccccagaga ccgtcacccg tggtgtccca
     3001 ctggaagagc gtgagcccgg tgctggcatc gagcccgtag aggatgccct tggagccacc
     3061 ctggtagaaa ctcgtggaat ttcccgggat cgtgctgacg tagacgatgc tgtcatagac
     3121 gaagggggcg atatcgatgc cctcgtactt gttgttgctg agctggacgc gccagttctc
     3181 cttgccggtc ttgccgtcga ccgaaacaac ctcggaggtg tctccgagtg ccccgaagac
     3241 ctggccatag gcgacggcga gaccgttcgg gccgaggaga ccgacgttgt attcggtctt
     3301 ccagttggtc ttgccggtcg ccttgtcgat cgaccagatg ttgctctgca tgtcgatgag
     3361 gtagatggtg tcatcgacga cgatcggtgt cgacgtcagc gaaccatagg ttcctggtgc
     3421 ctcgaccggg atgcgccagg cgacgccgag ggtgtcgatc gtcttcaggc tgatcgagga
     3481 gccggcggca ctgcgggttc cactgagatt cccatgttcg gtcggccagt tcgtctcgtt
     3541 cgaggtgaac tcggggggaa cggcagcacc cagtctggtg ggctgggcgg cggtctggtc
     3601 gatcgtgagt tgtgcttcct gcgacgcggg ggtggcgtcc ggggtggcct gggcggaagc
     3661 cagggcgtgt ccggcggcaa tcgttactcc ggcagcagtg gcggctccga caagcttacg
     3721 gcgactcatc agttcagcta acgtggacat tgcgactcct tttgtcccta tgcacccagc
     3781 cctcgtcggc agaatcctcg acacgtgtcc agaagc
//
LOCUS       DASTMD010000572         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2017607, whole genome shotgun sequence.
ACCESSION   DASTMD010000572 DASTMD010000000
VERSION     DASTMD010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017607"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..431)
                     /locus_tag="VFQ54_08400"
     CDS             complement(24..431)
                     /locus_tag="VFQ54_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0165051.1"
                     /translation="MTLLLKNVGALTIFVEDTQRTKAFYQERLGLPVAFEDATSVAFD
                     IGNTILNFIRYAEAPELIGPAPVAKPGAGGQFQLTIFVENADNVIADLEAAGVPLING
                     PIDRAWGMRTACFTDPDGYVWEIAQNISATPGS"
     gene            579..1073
                     /locus_tag="VFQ54_08405"
     CDS             579..1073
                     /locus_tag="VFQ54_08405"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0165052.1"
                     /translation="MTIPSETEALLGALNGQRKHLLGAIEGLPDDDLRRSVMPSGWTP
                     IALVQHLTIDVEQFWFRGVVGGEPFDLSLPADYGWTVAPDVPPGVILDRYRAEVAFGD
                     ALIAATDPDAAPKWWPEDLFGDYRLHTMREVVFHVMTETATHAGHLDIVRELIDRKQW
                     LVLT"
     gene            complement(1096..1935)
                     /locus_tag="VFQ54_08410"
     CDS             complement(1096..1935)
                     /locus_tag="VFQ54_08410"
                     /inference="COORDINATES: protein motif:HMM:NF005065.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0165053.1"
                     /translation="MAQRTWFITGVNSGFGREMTAQLLARGDRVAGTARRLDEMNDLK
                     TQYGELLWLAHLDVTDSDEIRRVVDAAFSELGRIDVVVNNAGYGLFGAAEELSDDQIL
                     HQLGTNLVGSIQVVRAATPHLRAQGGGRIIQISTMGGQAAWAGGSLYHAGKWGIEGFS
                     EALVTELAPFGIGVTMIEPGGARTNFRYGSAQLGASIAAYEGTPAGAIHGAITNRQAG
                     TPIGDPAKMVRIMIDSVEQDPAPKRIALGSDAYAAIHGALTERLATLEAQKDLAFSVA
                     AED"
     gene            2046..2621
                     /locus_tag="VFQ54_08415"
     CDS             2046..2621
                     /locus_tag="VFQ54_08415"
                     /inference="COORDINATES: protein motif:HMM:NF037675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEU0165054.1"
                     /translation="MDKGGETSRERILNAATAEFAAYGLAGARVDRIARAAECNKNLI
                     YIYYENKETLFTTVLQKHLLRVYDEIPFSGEDLPGYAAATFDFAMANPDVMRLLAWFA
                     LEQRAAHPEGRMAIHDVKVAAIAAAQEAGKVRTEFPPEFLVTAIMTLATAWSAASPFG
                     ATLDPESLRQPEELRRRIGAAIASIAAPTDD"
BASE COUNT          511 a          890 c          863 g          506 t
ORIGIN      
        1 gggcggcttc gtgatgttcg gtgtcaggaa ccgggcgtcg cggagatgtt ctgcgcgatc
       61 tcccagacgt agccgtcggg atcggtgaag caggccgtgc gcatacccca ggcccgatca
      121 atcgggccgt tgatgagcgg cacaccggcg gcttcgagat cggcgatcac gttatcggca
      181 ttctcgacga agatcgtcag ctggaactgg ccgcccgcgc ccggcttcgc caccggcgcc
      241 ggcccgatca actcgggcgc ctcggcgtac ctgatgaagt tgagaatggt gttgccgatg
      301 tcgaaggcaa ccgaggtcgc atcctcgaag gcgaccggca gcccgagccg ctcctgatag
      361 aacgccttcg ttcgctgcgt gtcctcgacg aagatggtga gcgcgccgac attcttcagt
      421 aagagcgtca tgaaatctcc tgcatccgcg catgcgatgg cggcaagacg ggtggcgaag
      481 gcggactcgc cgacgcccga actgcggcca ctatactggt gcccggaacg cggctgcttc
      541 tctcagcttg cgccggcatg gcgacaggag aaacgtcaat gacaattcca tccgagacgg
      601 aggcgctcct cggggccctg aatggccagc gcaagcatct cctcggcgcg atcgagggtc
      661 tcccggacga tgacttgcgg cgttccgtca tgccctcggg atggacaccc atcgcgctcg
      721 tgcagcacct gacgatcgac gtcgagcagt tctggtttcg cggcgtcgtt ggcggcgagc
      781 cattcgatct cagcctgccc gctgactacg gctggaccgt tgcccctgat gtcccgcccg
      841 gcgtcattct cgatcgttac cgggcggaag tcgcattcgg cgatgccctg atcgcggcga
      901 cggacccgga tgccgcgccg aagtggtggc cggaggacct cttcggcgac tatcgcctgc
      961 atacgatgcg cgaagtcgtg ttccacgtca tgaccgagac cgccacccac gccggtcatc
     1021 tcgacatcgt gcgtgaattg atcgatcgca agcagtggct cgtcctgacc tgatctctcg
     1081 taccaccccc ggaactcaat cctcggccgc gacggagaag gcgagatcct tctgcgcctc
     1141 gagcgttgcc aggcgctccg tcagcgcgcc gtgaatcgcc gcataggcgt cgctgccaag
     1201 cgcgatccgc ttcggcgccg ggtcctgctc gacgctgtcg atcatgatgc ggaccatctt
     1261 cgccggatcg ccaatcggcg tcccggcctg acgattcgtg atcgcaccgt ggatggcacc
     1321 ggcgggagtc ccttcgtaag cggcgatcga cgctccaagc tgcgcgctac cgtagcgaaa
     1381 gttcgtgcgt gccccgcccg gctcgatcat cgtgacgccg ataccgaacg gcgcgagctc
     1441 ggtcacgagc gcttcgctga aaccttcgat tccccatttt ccggcgtgat agagcgaccc
     1501 tccggcccag gccgcctgac cacccatcgt cgagatctgg atgatgcggc caccgccctg
     1561 cgcgcgcaga tggggtgtcg ccgcgcgcac gacctggatc gagccgacca ggttggtgcc
     1621 gagctgatgg aggatctggt cgtcgctcag ttcctcggcg gcgccgaaga gtccgtatcc
     1681 cgcgttgttc acgacgacat cgatccgccc gagttcgctg aaggcggcat cgacgacccg
     1741 acgaatctcg tccgagtcgg taacgtcgag atgagccagc cagagcagct caccatactg
     1801 cgtcttgaga tcgttcatct cgtcgagcct gcgtgcggtg ccggcgacac gatcgccacg
     1861 cgcgagaagc tgtgcggtca tctcgcgacc aaatccacta ttcacacccg tgatgaacca
     1921 cgtccgttgt gccacgttcc actccttgat cggctagact caaccagata gagctaactg
     1981 gttagtcaga gcatagtcct gtctaaccag ttagtcaata ggtatgacgt gaggaggagg
     2041 tccgattgga caagggcggc gaaacatcac gggagcggat tctgaacgcg gcgacggcgg
     2101 aattcgccgc ctacgggctc gccggagcgc gggtcgaccg tattgccagg gctgccgaat
     2161 gcaacaagaa tctgatctat atctattacg agaacaagga gaccctcttc acgaccgtcc
     2221 tccagaagca tctcctccgg gtttatgatg agattccgtt ctccggggaa gatcttcccg
     2281 ggtatgccgc ggcgaccttc gacttcgcga tggcgaatcc cgacgtgatg cgcctcctcg
     2341 catggttcgc cctcgagcag agggcggcgc atcccgaggg ccggatggcg attcacgatg
     2401 tgaaggtcgc ggcaatcgcc gcagcacagg aagccgggaa ggtgcggacc gagtttccgc
     2461 cggagttcct ggtgacggcg atcatgacgc tcgcgacggc gtggtcagcg gccagcccgt
     2521 tcggcgccac gctcgatccg gaatcgcttc ggcaacccga ggaactccgc cgcagaatcg
     2581 gagcggcgat cgcctcgatc gccgctccga cagacgactg acctcgcgga gcaacgacgg
     2641 aaagacggct cgagccacta acgaccgatt gcttgcccga tatacatcgc cacgatgcgt
     2701 tgcacgcgtt gggtgatgtc gggatcaatt gatccacgta ttcgacgaaa acgaaggaga
     2761 ctttgcgact
//
LOCUS       DASTMD010000573         1932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_767568, whole genome shotgun sequence.
ACCESSION   DASTMD010000573 DASTMD010000000
VERSION     DASTMD010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767568"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFQ54_08420"
     CDS             <1..259
                     /locus_tag="VFQ54_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165055.1"
                     /translation="VITADLGLLGQLRPGSRVRFVEVTVEEAFRLARNGDDVEEPGMS
                     LRDAVAMVRAFVALPFSALDLELPARDFSLRLRRGHQESPD"
     gene            534..1142
                     /locus_tag="VFQ54_08425"
     CDS             534..1142
                     /locus_tag="VFQ54_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0165056.1"
                     /translation="MESTGRTGRSLTRKGEATRERIVSGAAAEMRIRGVEHTSLDDVR
                     AVTSTSKSQLFHYFPEGRSQLLLAVAQREADLVIEDQMPYLDDLGSWEAWQSWRDLVV
                     ERYRSQGQECPLSILLSQIGRGYPETQSIVVNLMRRWQGAIATGIRTMQAKGEVKPAL
                     DADRSAAALLAGIQGGVVVMLATGEITNLEAALDVGIASLRV"
     gene            complement(1176..>1932)
                     /locus_tag="VFQ54_08430"
     CDS             complement(1176..>1932)
                     /locus_tag="VFQ54_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEU0165057.1"
                     /translation="AGMLYRDGARVALVGRPNVGKSSLLNALLRTERAIVTPIAGTTR
                     DVIEETINLEGIPATLLDTAGIVESEDLVERIGVERSRAALATSAAIVFVVDGSAAPQ
                     ESDLEIARTLASRSASEQTPIVIARNKADLDAHPDLAQLAGILPTAPVVPISALTGDG
                     LPDLEQALAEILRGGDGSGIRPAMITARQHAAIDRALVHIHDAQEARAAGIPQDLLAT
                     SVRAALHAVGEVTGEQVDEAVLHEIFSQFCIGK"
BASE COUNT          314 a          614 c          661 g          343 t
ORIGIN      
        1 ggtgatcact gccgatctcg gcctgctcgg gcagttgcga cccgggagcc gggtgcgctt
       61 cgtcgaggtg acggtggaag aggcattccg tctcgctcgc aacggggatg acgtcgagga
      121 gccggggatg tcgcttcggg atgcggtcgc gatggtccgc gcttttgtgg cactcccatt
      181 ctccgcgctc gatctcgaac tcccggcgcg tgatttctcg cttcgcctcc gtcgcggtca
      241 ccaggaatcg ccggactgat cacgcacacg tatcggccat tgacaataga gacatcgccc
      301 cggcgtatcc cgacgcgatc gacacctcgc gttccgatcc gccggggtct gtaggggaag
      361 accttgtgtc ttgccgcatg ccccacgccg catacaacgc ccctcgcggc aacgcgcatc
      421 caccgagacg ttgatgcatg cggtgacccc ggactagaat tggaccaaac ggttcacttc
      481 tctgaaggta acgaataagg aatggacggt caagtccaat cgggagtcgg tggatggaaa
      541 gcacgggcag gacgggacgt tccctgacgc gcaagggcga ggcgacacgg gagcggatcg
      601 tgagcggggc cgccgccgag atgcggatcc gtggggtgga gcacacctcg ctcgacgacg
      661 tgcgtgcggt caccagcacg agcaagagcc agctcttcca ctactttccg gaggggcgat
      721 cccagctgct gcttgccgtg gcgcagcgcg aggccgatct cgtgatcgag gaccagatgc
      781 cctatctcga tgacctgggt tcgtgggaag cctggcaatc ctggcgggat ctcgtcgtcg
      841 agcgctatcg atcgcagggc caggagtgcc cgctcagcat cctgctctcg cagatcggac
      901 gtggctatcc ggagacgcag tcgatcgtcg tcaatctgat gcgccgctgg cagggggcga
      961 tcgcgaccgg cattcgcacg atgcaggcga agggcgaggt gaaaccggcc ctggacgccg
     1021 atcgatcggc ggcggcgctc ctcgccggca tccagggcgg tgtggtcgtc atgctcgcga
     1081 ccggtgagat cacgaatctc gaggcagcgc tcgatgtggg gattgcatcc cttcgggtct
     1141 gatgtggtgg gtggcgccta cggctggtcg ttcctctact tcccgatgca gaactggctg
     1201 aagatctcgt ggagcacggc ctcgtcgacc tgctcgcccg tcacctcgcc gacggcgtga
     1261 agcgccgcgc ggacggacgt cgcaagaagg tcctgcggga taccggcggc acgtgcctcc
     1321 tgtgcatcgt gaatgtggac gagggcgcga tcgatcgccg catgctggcg ggccgtgatc
     1381 atcgccgggc ggatcccgct gccgtctccc cctcgcagta tttccgcgag ggcctgttcg
     1441 agatcgggaa gaccatcccc ggtgagtgcc gagattggca cgaccggcgc ggtcggcagg
     1501 atgccggcaa gctgtgccag atcaggatgg gcgtcgagat cggccttgtt gcgggcgatc
     1561 acgatcggcg tctgctccga ggccgagcgg ctcgccagcg tccgcgcgat ctcgagatcg
     1621 gattcctgtg gtgcggccga accgtccacg acgaagacga tcgcggcgga cgtcgccaac
     1681 gccgcgcgac tgcgctcgac accgatccgc tccacgagat cctctgactc cacgatcccg
     1741 gcggtgtcga gcagggtcgc cggaattccc tcgaggttga tcgtctcctc gatcacatcg
     1801 cgggtcgtcc ccgcgattgg tgtcacgatc gcgcgttcgg tgcggagcag cgcgttgagg
     1861 aggctgctct tgccgacgtt cggtcgcccg acgagggcaa cccgcgcgcc gtcgcggtag
     1921 agcatccctg cc
//
LOCUS       DASTMD010000574         1563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1214384, whole genome shotgun sequence.
ACCESSION   DASTMD010000574 DASTMD010000000
VERSION     DASTMD010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214384"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..>1563)
                     /locus_tag="VFQ54_08435"
     CDS             complement(25..>1563)
                     /locus_tag="VFQ54_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEU0165058.1"
                     /translation="GDVSIWDVSTGVRLRTMSHAGEKISFVNFSPDGKLIVTSGQEGT
                     DRVWNTGTGLLQVKLPTHSVIQPWVEFDRHSQLLVSGGSLVTVSDAHTGAPIAILEGS
                     KAGTTAVSFDATAHRIAGLAQDGTVQVWDASAQYKRWSSQPIDPDCATPISFDADRRF
                     VAVACKDHGSHIWDTGQDALIGDLPTVTPPDGNIFYRAAPVVSAAGDLAAIANGNNVE
                     IYQLPVDPDSGRKPRPIRTIYHHAQVNVVAFAPAGHALVSASVDGAVFVTTDDREPVE
                     ISKIAGGVDVAGFAPDGRVIVAATQGQLRIFDASSRTQLAATDLHMRGKAFRLSANGR
                     RLVIIPLPGDPSPAVLWALDANRMITKLDSHKGRLASARFVRGDREILTTGGDGVTRR
                     WDAETGGLLKWYVETSVVQLDAVLDPAGSMLLTAGIDGLVRFWDVSSGVLLWTLQAHK
                     GVNGLHFEGDRLVTRGMSGDLVRWEIPSAMSALESERLVRCLPQRFDESINGLVERAT
                     CDAL"
BASE COUNT          316 a          505 c          454 g          288 t
ORIGIN      
        1 caacagttgc cagcaggttc cggatcagag cgcgtcacat gtcgctcgtt cgactagccc
       61 attgatcgac tcatcaaagc gctgaggaag acaccggaca agtcgctcgg attcgagcgc
      121 cgacatcgcc gaaggaatct cccagcgaac gagatctccg gacatcccgc gcgtgaccaa
      181 ccggtcacct tcgaagtgga gaccgttgac gcctttgtgc gcttgcagcg tccacaacag
      241 tacgccgctc gacacatccc aaaaacgcac taatccatcg attcccgccg tcaggagcat
      301 ggatccagct gggtcgagga cggcgtccag ttgaacaacg gacgtctcga cgtaccattt
      361 cagcagaccg ccggtctccg cgtcccaacg cctggtaact ccatcgccgc ctgtcgtcag
      421 gatctcgcgg tcacctcgga caaaccgggc cgaggccagc cgacctttgt ggctatcgag
      481 cttggtgatc atgcggttgg catccagcgc ccagagcacc gcgggcgacg gatcgccagg
      541 aagcggaatg atgaccaagc gacgcccatt tgcggacagc cgaaaggctt tgccacgcat
      601 gtgaagatcg gtagcagcca gctgggtcct cgagctcgca tcgaagatcc ggagctggcc
      661 ctgagtcgcg gcgacgatca cccgcccatc gggtgcaaac ccggcgacgt cgacaccgcc
      721 tgcgatcttc gagatctcga ccggctcccg atcatcggtc gtgacgaaaa ccgcaccatc
      781 cacgctggcg ctgacgagcg cgtgtcccgc gggcgcaaac gcgacgacgt tcacttgtgc
      841 gtgatgataa attgttcgaa tcggccgtgg tttgcgtccg ctgtctggat cgacaggcag
      901 ctgataaatc tcgacgttat tgccgttggc gatcgctgcc aggtcacccg ccgcggagac
      961 caccggggct gcgcgataga agatgtttcc atccggcgga gtcaccgtgg gcaaatcccc
     1021 gataagcgcg tcctggcccg tgtcccagat atggctgccg tgatccttgc atgccacagc
     1081 gacgaatcgc cgatcggcat cgaatgaaat cggggtcgcg cagtcaggat cgatcggctg
     1141 cgagctccat cgcttgtatt gcgcgctggc atcccagacc tgtacggtcc cgtcctgggc
     1201 aagccccgcg atgcggtggg cggtggcgtc gaagctcact gccgttgtcc cggccttact
     1261 accttcgagg atggcgatcg gtgcgccggt gtgtgcgtcc gagaccgtga ccaacgaacc
     1321 accgctaacc agtaactgag aatgccgatc gaactccacc catggctgga taacgctgtg
     1381 ggtcggtagc tttacctgaa gcagtcccgt gcccgtattc cagactcggt ccgtaccttc
     1441 ttgaccggaa gtcacgatca acttgccgtc tggcgagaag ttgacgaacg agatcttctc
     1501 gcccgcatgc gacatcgttc tcagccgaac gcccgtcgag acatcccaga tcgagacatc
     1561 gcc
//
LOCUS       DASTMD010000575         3685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1662705, whole genome shotgun sequence.
ACCESSION   DASTMD010000575 DASTMD010000000
VERSION     DASTMD010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662705"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFQ54_08440"
     CDS             complement(<1..261)
                     /locus_tag="VFQ54_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0165059.1"
                     /translation="MSNGRVLIVDDEPQIRRALRTALAGHAYEVETAEDGDVALVQIA
                     TWQPDVVVLDLVMPGQDGFAVVRDVREWSQVPIIVLSARGQET"
     gene            complement(283..1959)
                     /locus_tag="VFQ54_08445"
     CDS             complement(283..1959)
                     /locus_tag="VFQ54_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0165060.1"
                     /translation="MKTGPFDESNRESEDGVPRRWPRMPTKREIVATARLTWRGYVGA
                     LIAVAIVSAVMLPVRSPLGVINIVLIYVALSLALGLVLGAGPASIGSVVAFFAFDFLF
                     IPPYYTADVANRHHVFALFVYLAISVVTSVLVSRVRARAEEARRESHRTTLLYDLNRA
                     LVQGVTLDQILQTIVENVVLIYGSMGCRVLVRDGEGLRSRASSPPQEGPVDRQAATIA
                     LWVIEHRLPAGLSSEGRRIRQPHGRGLPPKEPLTRKQNDVLYVPVATDERVIGVLEVS
                     GKPGGGRFAEEDARLLTSFADQAALAVERARLADEEARARVLEQTNELKSALLAAVSH
                     DLRTPLAAIKASATALLDTSVEWDTAARDELLSAIDGETDRLTLMVSNLLDLSRIEGG
                     ALRPDKDWHDIGELLADAQVRLLRQTEDHPLTIDVAPDLPVVSFDYVEIAQVVLNLIG
                     NAAKYSPAGSPILVTAQSVGSEVQISVRDHGMGIPPDRLPHIFETFYRAHEHGPVAGS
                     GIGLAICKGLVEAHGGRIWAQSRVGEGTMVTFSLPNPRPVLTPLTEEIPA"
     gene            2044..2598
                     /locus_tag="VFQ54_08450"
     CDS             2044..2598
                     /locus_tag="VFQ54_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0165061.1"
                     /translation="MTARLTIPILRGARITLRPFTPDDAPTVQEMLAGPEIASTTLNI
                     SHPYPEGAAASWIESHAEAADLGMALTWASERTEDSMLLGAIAVHLTPRHLRGEIGYW
                     LGVPFWNQGYTTEAAKLVVDYAFATLGLHRVQATCLPTNIGSSRVMEKAGLTFEGILR
                     DYYQKRGVFTDVAMYARIARQDTE"
     gene            2617..>3685
                     /locus_tag="VFQ54_08455"
     CDS             2617..>3685
                     /locus_tag="VFQ54_08455"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0165062.1"
                     /translation="MSTAIEADPDLDLEPLIAGILARNETPGASIATIADGVHRSMAV
                     GSSDLDGHQPLGVDARFPIYSITKTFIATAILKLVEAGSFPLDLPARAFLPGVPLDPA
                     ITIRQLLNHTSGLPDYGRLASYHDDLRRDPTAAWSPQEFLERTLAQGPLFPPGQGWQY
                     SNIGYLILNRVLDYAMWTSFADALTQLVITPAELSRTSVATSLESMRILTPGFSTALN
                     DAADDRPRNIAPRYHPGWVSHGLVLSTATEVATFFDALFGGRLLTPSSLAAMLDPVRV
                     PIDRHPFFVTPSYGLGLMLDPGSPHGLIAGHGGGGPGYSLGAVRIIPSEGGRAITSIA
                     LTNRDRGDVGLEIAWTIADAIA"
BASE COUNT          656 a         1275 c         1132 g          622 t
ORIGIN      
        1 cgtctcctgc ccacgcgcgg agaggacgat gatcggcacc tgcgaccatt cgcggacatc
       61 gcgcaccacg gcgaacccat cctggcccgg catcacgaga tcgaggacga cgacatctgg
      121 ctgccaggtc gcgatctgga cgagtgcgac gtcgccgtcc tcggcggtct ccacctcgta
      181 ggcatgaccg gccagcgcgg tccgtagcgc acgccggatc tgtggctcgt catcgacgat
      241 caggactcgc ccgttactca ccaacgctcc attcccgaat gctcaggccg gaatctcctc
      301 ggtcaacggt gtcaggacgg gacggggatt cgggagcgag aatgtgacca tcgttccctc
      361 cccgacccgg ctttgcgccc agattctccc gccatgggct tcgaccagcc ccttgcagat
      421 cgccagcccg atgccggaac ccgccaccgg gccatgctcg tgggcacgat agaacgtctc
      481 gaagatatgc ggaagtcgat ccggcgggat tcccatcccg tgatcccgca cgctgatctg
      541 gacctcacta ccgacggact gcgcggtaac gaggatcggg gaccccgccg gggaatactt
      601 cgccgcattc ccgatcaggt tgagcacgac ctgggcgatt tccacgtaat cgaacgaaac
      661 caccggcaag tcgggcgcga cgtcgatcgt cagcgggtga tcctccgtct ggcgcagcag
      721 gcggacctgc gcatccgcga gcaattctcc gatgtcgtgc caatccttgt ccggccgcaa
      781 ggcgcccccc tcgatccgcg agaggtcaag gaggttgctc accatcagcg tcaggcgatc
      841 ggtctccccg tcgatcgcac tcagcagttc atcgcgcgcc gccgtgtccc attcgaccga
      901 ggtgtcgagc agcgccgtgg ccgatgcctt gattgcggcc agcggtgtgc gcaggtcatg
      961 cgagaccgcc gcgagcaacg cggacttcag ctcgttcgtc tgctcgagga cacgcgcccg
     1021 cgcttcctcg tccgcgagtc gtgcccgctc cacggcgagc gcggcctgat cggcgaagct
     1081 ggtcagcagg cgcgcatcct cctcggcgaa gcgaccaccc ccgggttttc cactcacctc
     1141 gagaaccccg atgacccgct cgtcggtcgc gaccgggacg tagagcacgt cgttctgctt
     1201 gcgcgtcagc ggttccttcg gcggcaggcc acgtccatgc ggttggcgga tgcggcgccc
     1261 ctccgaactc aagccggcgg gcaggcgatg ctcgatcacc cagagcgcga tcgtcgccgc
     1321 ctgccgatcg accggtccct cctggggcgg cgaggatgcc cggctccgca acccctcgcc
     1381 gtcacgtacg agcacgcggc atcccatcga cccgtagatc aggacgacgt tctcgacgat
     1441 cgtctgcagg atctggtcga gcgtgacgcc ctgaaccaac gcccggttga gatcgtagag
     1501 cagcgttgtc cggtgggatt cccgccgcgc ctcctcggcc cgggcccgga cgcgggatac
     1561 gagcacactc gtcaccacgg agatggcaag atagacgaag agcgcgaaga cgtggtgcct
     1621 gttggcgaca tcggcggtgt aatacggcgg gatgaagaga aaatcgaagg cgaagaaggc
     1681 cactaccgag ccaatcgacg ccggtccggc tcccagcaca agtcccagcg ccagggagag
     1741 ggcaacgtag atcagcacga tgttgatgac accgaggggg ctgcggaccg gcagcatcac
     1801 ggcggaaacg atggcgaccg cgatcagcgc gccaacgtag ccgcgccagg tcagacgcgc
     1861 ggtcgccacg atctcccgct tcgtcggcat tctgggccaa cgacgtggga cgccatcctc
     1921 actctcacga ttcgactcgt cgaatggtcc cgtcttcatc acacgtatcc gatcacccga
     1981 taccgcatcg aatccgcgcg atagtggtcc gctcccggca agggtagaac atggagttcc
     2041 gcaatgacag cgcgcttgac gatcccgatc ctgcgcggag cacgcatcac cttgcgcccc
     2101 ttcacacctg atgacgcgcc aacggtacag gagatgctcg ccggtccgga gatcgcgtcg
     2161 accacgctga acatcagcca tccctatccg gagggggccg cggcatcctg gatcgaatcg
     2221 cacgccgagg cggcggacct ggggatggcg ttgacgtggg cgagcgaacg cacggaggat
     2281 agcatgctgc tcggtgcgat cgcggtccac ctgacgccgc ggcacctccg gggagaaatc
     2341 ggctactggc tcggcgtccc cttctggaac cagggctata cgacggaggc cgcgaagctc
     2401 gtcgtcgact acgccttcgc cacgctcggt cttcatcgcg ttcaggccac ctgcctgccg
     2461 acgaatatcg gttcgtcgcg cgtgatggag aaggcgggtc tcacattcga gggaatcctg
     2521 cgcgattatt accagaagcg cggcgtcttc acggacgtgg cgatgtacgc aagaatcgca
     2581 cgacaggaca ccgaatgaac cgaatgaatg gaaagcatgt caacggcaat cgaagctgat
     2641 cccgatctcg acctcgagcc gctcatcgcc gggatcctcg cccgcaacga gactccggga
     2701 gcctcgatcg cgacgatcgc cgacggcgtg catcgctcga tggctgtcgg ctcgagcgat
     2761 ctcgacgggc accaaccgct gggagtcgat gcacgtttcc cgatctacag catcacgaag
     2821 acgttcattg caacggcaat cctgaaactg gtcgaggccg ggagcttccc cctcgatctc
     2881 cccgcacggg cgttccttcc cggtgtcccg ctggatcccg ctatcacgat ccgtcagctc
     2941 ctcaaccata cgtccggcct gcccgattac ggccggctcg ccagctatca cgacgacctg
     3001 cgccgcgacc ccacggccgc ctggtcaccg caggagttcc tcgagcggac gctcgcccaa
     3061 ggaccgctct tcccgccggg tcagggatgg cagtattcga atatcggcta cctgatcctg
     3121 aatcgagtgc tcgactatgc gatgtggacg tcgttcgccg atgccctgac ccaactggtc
     3181 atcacgccgg cggagctctc gcgaacatcc gtcgcgacct cgctcgaatc gatgcggatc
     3241 ctgacgccgg ggttcagcac cgcgctgaat gacgctgctg acgatcgacc gcggaacatc
     3301 gccccgcgtt accaccccgg ttgggtctcg cacgggctcg tcctctccac cgcgaccgag
     3361 gtcgcgacgt tcttcgacgc cctcttcggc ggacgtctcc tcaccccgtc ctcgctggcg
     3421 gcgatgctcg atcccgttcg cgtgccgatc gaccgccatc cgttcttcgt gacgccgtca
     3481 tatggactcg ggttgatgct cgatccgggt tcgccccatg gactcatcgc gggtcacggc
     3541 gggggcgggc caggctattc actgggtgcc gtccggatca ttccctccga ggggggacgc
     3601 gcgatcacga gcatcgcgct taccaatcgc gaccggggag acgttgggct ggagattgcc
     3661 tggacgattg ccgatgccat cgccc
//
LOCUS       DASTMD010000576         1923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2110008, whole genome shotgun sequence.
ACCESSION   DASTMD010000576 DASTMD010000000
VERSION     DASTMD010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110008"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VFQ54_08460"
     CDS             <1..955
                     /locus_tag="VFQ54_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEU0165063.1"
                     /translation="STADTTSSSDGASNDGATGTWHTNDSVNLRSGPSLGDSVITELS
                     VSTDVNLTGTTQNGFAQASTDSGDGWISQDYLAEGAAAADTSQDAPAASAVSTDGPTG
                     TWHTNDSVNLRSDASLGSSIVDVLAASTDVTLTGATSDGFAQANTDSGPGWISQDYLA
                     EGGAQSNASQATASTADASTSSSDAAASDGGSSNEGQALVDYAMQFLGQAYVWAGNQP
                     GGFDCSGLTQYVVQNVLGYDIGHGTAGQTAFGTPVAWGDWQPGDLVFFQNTYDVGITH
                     VGIYIGNDQMINAENPDTGVTISDVTDAYYTAHYYGAYRLV"
     gene            1096..1593
                     /locus_tag="VFQ54_08465"
     CDS             1096..1593
                     /locus_tag="VFQ54_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0165064.1"
                     /translation="MTPEERRTAIDQYERGADELEAALSEAPDEARKWRPAPGDWTIH
                     EIVVHCADSETSGYTRIRMMAAEPEPIIVGYDQDAWTTYFDYHSIPLDRSLAVIRAVR
                     AHTAPLLRTLPEEAWTKQGTHTQSGPYFTTDWLAIYSVHLSDHADQIRRNLKSWREHA
                     SESPE"
     gene            1624..>1923
                     /gene="purH"
                     /locus_tag="VFQ54_08470"
     CDS             1624..>1923
                     /gene="purH"
                     /locus_tag="VFQ54_08470"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287218.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU0165065.1"
                     /translation="MRALLSVYDKSGIVAFARELDHLGYELVSTGGTFAALEAAGLPV
                     RQVSDVTGSPEILDGRVKTLHPKIHGGLLARRDLPDHVRQLEEHEITPIDILVSNL"
BASE COUNT          360 a          647 c          593 g          323 t
ORIGIN      
        1 gtcgaccgca gacacgacat cttcctccga cggggcctcg aatgacggcg ccacgggaac
       61 ctggcacacg aacgactccg tcaatctccg ctccggcccg agtctcggcg actccgtgat
      121 cacggagctg tcggtctcga ccgatgtcaa tctcacgggc acgacccaga acggcttcgc
      181 ccaggcgagc accgacagtg gcgacggctg gatctcgcag gactatctgg cagagggtgc
      241 cgccgcggca gatacctccc aggatgcgcc ggcggcgagt gccgtcagca cggacggccc
      301 gacggggacc tggcacacga acgattccgt caatctccgc tcggatgcga gtctcggctc
      361 ctcgattgtc gacgtgcttg ccgcttcgac cgacgtgacg ctgacgggcg cgacttcgga
      421 cggattcgcc caggcgaaca ccgacagcgg tccaggctgg atctcgcagg actaccttgc
      481 ggagggtggc gcgcaatcca acgcgtccca ggccaccgca agcaccgccg acgcctccac
      541 gagttcttcc gatgcggcgg cgtcggatgg cggaagttcc aacgagggac aggcgctcgt
      601 ggactatgcg atgcagttcc tcgggcaggc gtacgtctgg gcaggcaatc agcccggtgg
      661 attcgactgc tccggtctca cccaatacgt tgtccaaaac gttctgggtt atgacatcgg
      721 gcacggcacg gccggtcaga ccgcattcgg gacaccggtc gcatggggtg actggcaacc
      781 cggcgacctg gtcttcttcc agaacaccta tgacgtcggc atcacccacg tcggcatcta
      841 catcggcaac gatcagatga tcaatgccga gaatccggat accggcgtca cgatctcgga
      901 cgtcacggac gcctactaca ccgctcacta ctacggtgcc taccggctcg tctagagggt
      961 gatggcgaga acgcagggca gggaggcggg cggacgagag tctgcccgcc tctccgcggt
     1021 tgcgggaccg tcattccttg ggtaggctgt cggcgactcg gatcgacgtt ctcgacacag
     1081 gagatcttgt tccacatgac cccagaagaa cggcggacag cgatcgatca gtacgagcga
     1141 ggcgcggatg aactcgaggc ggcgctttcg gaggcgccgg acgaggcgag gaagtggcgc
     1201 ccggcgccgg gcgactggac catccacgag atcgtcgttc actgtgccga ttccgagaca
     1261 tccggctaca cccggatccg gatgatggcc gccgagccgg agccgatcat cgtcggctac
     1321 gatcaggacg cctggacgac gtatttcgac tatcattcga ttccgctgga ccggtcgctt
     1381 gcggtaatcc gcgccgtccg tgcccacacc gcgccgctgc tccgcacgct tcccgaggaa
     1441 gcctggacaa agcagggcac gcacacgcaa tccggcccct acttcacgac ggactggctc
     1501 gcgatctact cggtgcatct ttccgatcat gccgaccaga tccggcgcaa tctcaagtcg
     1561 tggcgcgagc acgcgtccga atctcccgaa tagatacgct cacaggtaaa ggatccccat
     1621 ctcgtgcgtg cgctcctcag tgtgtatgac aagtccggta tcgtcgcctt cgcgcgggag
     1681 ctcgaccatc tcggctacga gctcgtctcg accggcggga catttgccgc gctggaggcg
     1741 gccgggctgc ccgtccgcca ggtctccgac gtgaccggat cccccgagat cctcgatgga
     1801 cgcgtgaaga cgcttcaccc gaagatccac ggcggcctgc tcgcacgccg cgaccttccc
     1861 gatcacgtcc ggcaactcga ggagcacgag atcacgccga tcgatatcct cgtctcgaat
     1921 ctc
//
LOCUS       DASTMD010000577         1841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_678441, whole genome shotgun sequence.
ACCESSION   DASTMD010000577 DASTMD010000000
VERSION     DASTMD010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678441"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..964)
                     /locus_tag="VFQ54_08475"
     CDS             complement(230..964)
                     /locus_tag="VFQ54_08475"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0165066.1"
                     /translation="MTSSAIFNTDGPRAFDLIRHPKNGQTTLYLVRHGQTEGNAKHLF
                     IGSTDLPLDPLGERQAVQVGDHFATIEIDHLVSSPMLRARQTAAEISRSTGKEIEIVH
                     TLSEIDFGAIEGSTIEQVVTQYPELRSRLDDPDDLDVQWPDGESRRGFNDRILTSFLG
                     ILEQYVNKTVAVVCHGGVIGSFIAQVEGGSPSDFARYAVRNCSVTNLVVTPNQTLIHF
                     TNDIEHLDEVQLEPLRLSPNLMKSNP"
     gene            complement(936..1619)
                     /gene="nth"
                     /locus_tag="VFQ54_08480"
     CDS             complement(936..1619)
                     /gene="nth"
                     /locus_tag="VFQ54_08480"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU0165067.1"
                     /translation="MNSRSDSRIRLAIVADLINETYGPYALHPNADPLGELIATILSQ
                     NTSDVNMERSYRSLRETFATWDEIVSADTAAVIAAIRIGGLANSKAPRIQSVLRDIRE
                     RVGGDDLASLATMSLDAAKAWLTSLPGVGPKTAACVLLFSLGMPAMPVDTHVHRLSRR
                     IGFVGPKVSAEETEDVLETLLGDNPDRIYSLHRGLLAHGRTICRARTPNCADCILKEH
                     CDFFRDLQH"
BASE COUNT          338 a          517 c          615 g          371 t
ORIGIN      
        1 tgacccgttc ccacacccgc cgcgctgcca ccgaggatcg tcagtttcgc catgggaatc
       61 gacctttctg gtctcatgag agcgcgtgaa cgaggcgttc tattccgcta tgaacgtcaa
      121 cgagcggggc ggaagctcct gatcgtcagg gggagggagg tgttgacgca acatcgctca
      181 tggaacgggt atatcgagct gcgccggatg ggtcaggcca gccgacccgc tacggattcg
      241 atttcatcag gttcgggctg agacgcaacg gttcgagttg cacctcgtcg agatgctcga
      301 tgtcgttcgt gaaatggatc agcgtctgat tcggggtgac gacgaggttg gtgacgctgc
      361 agttgcggac ggcgtagcga gcaaagtcgc tcggtgagcc gccttcgacc tgggcgatga
      421 acgagccgat gacgccaccg tggcagacga ccgcgaccgt cttgttaaca tactgttcga
      481 ggatgccaag gaatgaggtc aggatgcggt cgttgaatcc gcgccgtgac tcgccgtccg
      541 gccactggac gtcgagatcg tccggatcgt cgagacggct gcgaagttcc ggatactgcg
      601 tgacgacctg ttcgatcgtg gaaccctcga tcgcgccgaa gtcgatctcg ctgagggtat
      661 gaacgatctc gatctccttg ccggtcgaac ggctgatctc ggcagctgtc tgcctggcgc
      721 gcagcatcgg gctcgagacg agatggtcga tctcgatcgt ggcaaagtgg tcgccgacct
      781 ggacggcctg gcgctcaccg agcggatcga gcgggagatc ggtcgatccg atgaagagat
      841 gcttcgcgtt tccctctgtc tggccgtggc gaacgagata gagcgtcgtc tggccgttct
      901 tcgggtggcg gatgagatcg aaggcgcgtg gcccgtcagt gttgaagatc gcggaagaag
      961 tcacagtgtt ccttcaggat gcaatcggcg cagttcggag tccgcgccct gcagatggtc
     1021 cgtccgtggg cgaggaggcc ccggtgcagc gaatagatcc gatctggatt atctcccaga
     1081 agagtctcga ggacatcctc cgtctcctcg gcggagactt tcgggccaac gaatccgatg
     1141 cgccgggaaa gacggtggac gtgggtgtcg accggcatcg cgggcatccc gagtgaaaag
     1201 aggaggacgc acgcggcggt cttcggtccg acgcccggga gcgacgtcag ccacgctttc
     1261 gccgcgtcga gactcatcgt ggcgagcgac gcgagatcgt ctccgccaac gcgctcgcga
     1321 atatcgcgga gaaccgactg gatgcgcggc gccttgctgt tcgcgaggcc accgatccgg
     1381 atcgcggcga tcacggccgc ggtgtcggcg gagacgatct cgtcccaggt cgcgaatgtc
     1441 tctcgaagcg agcgatacga gcgttccatg ttcacatccg aggtgttctg ggagaggatg
     1501 gtcgcgatga gttcccccaa cggatcggcg ttcgggtgca acgcgtatgg gccgtacgtc
     1561 tcgttgatga gatcggcgac gatcgcgagg cgtattcggg agtcggagcg gctattcatg
     1621 tcgtgagcct gtgaagtttg cattggcctg gccgagacca tatactagag cgattttgct
     1681 tcggtctctg tggagtacca aaccccctgt gccgcgaaca cctttgacca taatcccgaa
     1741 tctgcctcgc cctcgggtcc ccgttggacc tcgggggtca ttttttatgg acggccatcg
     1801 ctgtcactcg aacattgtac ggagaggaat gcgatgatgc c
//
LOCUS       DASTMD010000578         3575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3180462, whole genome shotgun sequence.
ACCESSION   DASTMD010000578 DASTMD010000000
VERSION     DASTMD010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3180462"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFQ54_08485"
     CDS             complement(<1..703)
                     /locus_tag="VFQ54_08485"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0165068.1"
                     /translation="MTTTSFQDALAAIDGWIADGAVGGVSAAVWHKGEIVAERQAGEA
                     QPGIPVTADTLFALASVSKPFTATAVMRLVDRGEFSLDTPIAAIVPEFGETDDPFADD
                     AYPQLEALRDRVTFRHVLSHTSGLPENIGVKRLRMREQPSLTRMLDVMCGLPLQDVPG
                     EVLRYSNVGMGVAARAAERAAGIDFLDLLQAEVLTPWDLPGVIARPAEDIDASIAIVQ
                     DPSGGGTPIESYNSRY"
     gene            854..1624
                     /locus_tag="VFQ54_08490"
     CDS             854..1624
                     /locus_tag="VFQ54_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU0165069.1"
                     /translation="MGSATSVESGTSFSIEVVESYDKLSALAADNVSEFLAAHPTAAF
                     TLPTGETPVGMYRELVRRADAGEIDFSRAQFFCLDDYLGATPEDEASLTRWLTDIFLV
                     PAHLPQENIHYIPSTAEDPTAAAAAYDRAIADLGGFQLAVVGLGPNGHVGFNEPGSRP
                     DDPTRVVDLQPATRAQSAAYWEGRAEIPAKAMTTGLATILAAKKIVLIVSGKGKAEIV
                     RASLEGPETADVPGSWLRRAGDRLHVILDKDAASLLSL"
     gene            complement(1851..2747)
                     /locus_tag="VFQ54_08495"
     CDS             complement(1851..2747)
                     /locus_tag="VFQ54_08495"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEU0165070.1"
                     /translation="MTSVSSIAYTRELDVALEAARRAAAVILGFYEARTAETYTKGDG
                     SPVTDADLAADQVIRETIAAAFPEDAMLTEEGAKDLARIENPRCWIVDPLDGTEQFVK
                     GTDRFEVFVALSENGRPVVAVSAHPPTGVLHAAVIGQGAWRIDGDVRQPLTFAPAARP
                     PILASSKWYRGREGREMILRVAETLGSAAPPILEVGFQPRDFDEGLRTYDAFIGLWPE
                     AGQSIAHEWDLAASDLIVNEAGGRFTDLWGRPHQYNKRDTTTSGGILVTATVELQERI
                     LAAFATERPEIAPPPDPADNRQ"
     gene            3304..>3575
                     /locus_tag="VFQ54_08500"
     CDS             3304..>3575
                     /locus_tag="VFQ54_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165071.1"
                     /translation="MTISPRSLFRNRRVYGVVVLGLSSFLLVSCGGSSDKDKTPTAAP
                     PTEVVQAAPTEVATIPPAPTSPPSPVAGPSTPGASSPVASPSTIGP"
BASE COUNT          616 a         1129 c         1177 g          653 t
ORIGIN      
        1 agtagcggct gttgtaggac tcgatcggcg ttcccccgcc ggacggatcc tgaacgatcg
       61 cgatcgatgc atcgatatcc tccgccgggc gtgcgatcac gcccgggaga tcccagggtg
      121 tcagcacctc agcctgcagc aggtcgagga agtcgattcc cgcggcgcgc tccgccgccc
      181 gcgcggcgac tcccatcccg acgttggaat accgcaggac ctcgcccggg acgtcctgca
      241 gcgggaggcc acacatgacg tccagcatcc gcgtcagcga cggttgctcg cgcatccgga
      301 gccgcttcac gccgatgttc tcggggagtc ccgacgtgtg cgacagcacg tggcggaagg
      361 tgacgcgatc ccgcagtgcc tccagttggg gataggcgtc gtccgcgaac ggatcgtcag
      421 tctcgccaaa ctcggggacg atcgccgcga tcggcgtatc cagcgagaac tcaccgcgat
      481 cgacgaggcg catcacggcg gtggcggtga atggcttcga aacggatgca agcgcgaaca
      541 gggtgtccgc ggtcacgggg atgcccggtt gagcctcccc ggcctggcgc tcggcaacga
      601 tctcaccctt gtgccagacg gcggcggata caccacccac ggcgccatcg gcaatccagc
      661 cgtcaatcgc cgcgagcgcg tcctgaaagg aggttgtggt catggggagc gattcctcgg
      721 ggcacacggc gcggcgcatg ccgattctga gaattgggta tgatgaaggc gacgcgaaag
      781 acggctgttg ttgcagcccc cacgcggact gacgctattg tatgagtgat acgaccagag
      841 gatgacaggc cagatgggat ccgcaacatc cgttgagtcc ggaacctcgt tctcgatcga
      901 agttgttgag agctacgaca agctgagcgc gctcgccgca gataacgtct ccgagttcct
      961 ggcggcgcat ccgaccgcgg cgttcacgct cccgaccggg gaaacacccg tcgggatgta
     1021 ccgggaactc gtgcgccggg ccgacgcggg cgagatcgac ttctcgcgag cccagttctt
     1081 ctgcctcgac gactatctcg gtgcgacgcc cgaggatgag gcgagcctga cccgctggtt
     1141 gacggatatc ttcctcgtcc ccgcgcatct cccccaggag aacatccatt acatcccgtc
     1201 gacggcggag gatccgacgg cggctgcggc cgcgtacgat cgcgcgatcg ccgatctcgg
     1261 tggattccag ctcgcggtcg tcggtctcgg tccgaacggg cacgtcggct tcaacgagcc
     1321 cggttcgcgg cccgatgatc cgacgcgcgt cgtcgatctc cagccggcga cgcgagccca
     1381 gtcggcggcg tactgggaag gtcgcgcgga gatcccggcc aaggcgatga ccaccgggct
     1441 ggcgacgatt cttgcggcga agaagatcgt gctgatcgtc tccggcaagg gcaaggccga
     1501 gatcgtgcgc gcgtcgctcg aggggccgga gacggctgat gtgccaggct cctggttgcg
     1561 acgggcgggc gatcgcctcc acgtgatcct cgacaaggat gccgcctcac tcctttcgtt
     1621 gtaaccaact gccgtcgttg cgacgagaac gcccctgatc gaaagcttcg atcaggggcg
     1681 tctctgtgtg gaaggcccta gtggtctgtc acctgtgcta ctccggttgc catcgcatgc
     1741 caacgatcat atgcggcgaa gccgcatggg ccacacaccc ttcggcgaac gcagggcagg
     1801 ctcgttggcg gcccgctccg agatccccgt ggccattcag ctgtgacgca ttactggcga
     1861 ttgtccgcgg gatcgggcgg cggcgcgatc tctggccgct ccgtcgcgaa cgcggcgaga
     1921 atccgttcct gcagttcgac cgtcgccgtg acgaggatgc cgccactcgt tgtcgtgtcg
     1981 cgcttgttgt actggtgagg gcgaccccag aggtcggtga agcgcccgcc ggcctcattg
     2041 acgatcaggt cggatgccgc gaggtcccat tcgtgcgcga tcgactgacc tgcttccggc
     2101 cagagtccga taaatgcatc gtacgtacgc aggccctcgt cgaaatcccg cggctggaaa
     2161 ccgacttcga gaatcggggg cgcggctgat ccgagcgtct ccgcaacgcg aaggatcatc
     2221 tctcgccctt cgcgaccgcg gtaccacttc gacgaggcga ggatcggtgg acgggcggcg
     2281 ggtgcgaagg tcagcggttg gcggacatcg ccgtcgatgc gccaggctcc ctggccgatg
     2341 acggcggcat gcaacacgcc ggtcggagga tgggcgctga ccgcgacgac gggacgtccg
     2401 ttctcggaga gggcgacaaa gacctcgaag cggtccgtgc ccttgacgaa ctgctccgtg
     2461 ccgtcgaggg gatcgacgat ccagcaccgc ggattctcga tccgggcgag gtccttcgct
     2521 ccctcctcgg tgagcatcgc gtcctccggg aaggcggcgg cgatcgtctc gcggatcacc
     2581 tggtcggcgg cgaggtcggc atcggtgacg ggcgagccgt cgcccttggt gtaggtttcg
     2641 gcggtgcggg cctcgtagaa gcccaggatc acggcggcgg cgcggcgtgc ggcttcgagc
     2701 gcgacgtcga gttcccgcgt ataggcaatc gagctgacgg acgtcacttt gatcaaactc
     2761 cttcacgttc tctgtgtttt gctccgccac ccgattgggt caggacagcg gcacacgagc
     2821 ggtgatatcg gcgagtgctt ccgaacgttc acgagcaatc ccgtcgcttg gcggtgttgc
     2881 ccggtctctt tgcgataaac tttcaacgag agggaaccgt cggcccaact tcgtcgtatc
     2941 ctcgacacgg gtgtgttgcg ccgggtgatt gataaccaga ccgtagttat caaacttcaa
     3001 cccagtgtaa actctgcgac agcgccttct ggatcgagtc cgaggctcga atcctgcggc
     3061 acacgtcaga aacgagttcg ttcgtgcttt cgtgggagaa aaccgaaagt acgcaagatg
     3121 atgcgtggtt tctccggccg ctgggggcga gatgaaccac ctgcagcatc gtgacgaaca
     3181 ggctcgtaac tccaggaaac ggacacgatc cgcgtgtgga tcaggttcca tgggttatct
     3241 cttcgcgtct ttgacgcgcg gggcaagtcg acagcgttca cttttttggg agggttcgtt
     3301 aacgtgacca tttcacctcg ttcacttttc cgcaatcgac gcgtttatgg cgtcgtcgtg
     3361 cttggcctga gttcgttcct gctcgttagc tgtggcggga gtagcgacaa ggacaagaca
     3421 ccgactgccg cgccaccgac cgaggtcgtg caggctgctc cgacggaggt cgcaacgatc
     3481 ccgccggctc cgacatcgcc gccgtcgcca gtggccggcc catcgacacc aggcgcgtcg
     3541 tcgcctgtag cgtcgccatc gacgattggt ccgga
//
LOCUS       DASTMD010000579         1859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_947170, whole genome shotgun sequence.
ACCESSION   DASTMD010000579 DASTMD010000000
VERSION     DASTMD010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947170"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1375
                     /gene="larC"
                     /locus_tag="VFQ54_08505"
     CDS             176..1375
                     /gene="larC"
                     /locus_tag="VFQ54_08505"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEU0165072.1"
                     /translation="MKIAYFDPFSGASGDMILGALVDAGLPLSRLAAELGKLGVDGYD
                     LSARQTGQHGIHGTRVVVRETEHAHGRTWSDIRALIERSELDAPIRDAAISIFQRLAE
                     AEAKIHASTPEEIHFHEVGGIDAIVDICGACIGLALLGIEEVYAGPLQVGSGFARSAH
                     GIIPVPAPATAELLANAHAPIARPLPEMNERPAELLTPTGAAILTTLAEFRRPSFVPS
                     AVGFGFGTMELPWPNALRVWIGETASTSDAGDDGELVLETNIDDMNPQFIELVMERLF
                     AAGALDVWLTPITMKKGRPATTISVLAAASKRHDLERILIENTTTLGIRATAIDRVKV
                     PRAFETVATRWGDIPLKLKIWNGRVIDISPEYDACVAIARHHDLPVKDIWNEAYRLGE
                     SLIGQRR"
BASE COUNT          346 a          629 c          586 g          298 t
ORIGIN      
        1 ccgatcccct gtgatctgtg aatggaagag atcttagcat gcgcgttacg tcaaacacgc
       61 ccggacgaac ggcgggttcg gggaacgatt ggtatcctgc atgtgcgaag cgcccagttc
      121 acccgggccc ccatgttcag ctacgacgtg aaccccatcg aggagcactc tcccagtgaa
      181 gatcgcctac ttcgacccgt tctccggcgc cagcggggac atgatcctcg gcgccctggt
      241 tgacgccggg ctcccactct cccgcctcgc cgccgagctc ggcaagctgg gcgtcgacgg
      301 ctacgacctc agcgcccggc agacgggtca gcacggaatc cacggcaccc gggtcgtcgt
      361 tcgcgagacg gaacacgccc acggtcgcac ctggagcgac atccgggccc tgatcgaacg
      421 atcggaactc gacgcgccga tcagggacgc cgcgatctcg atcttccagc gacttgccga
      481 ggcggaagcg aagatccatg cctcgactcc ggaggagatc cacttccacg aagtcggcgg
      541 catcgacgcg atcgtcgaca tctgcggcgc ctgcatcggc ctcgccctcc tcggtatcga
      601 ggaggtctac gccggaccgc tccaggtcgg atcgggattc gcccgcagcg cgcacgggat
      661 cattcccgtt cccgccccgg cgacggcgga actcctcgcg aacgcgcatg ccccgatcgc
      721 ccgcccgctc cccgagatga acgagcgacc ggcggaattg ctcaccccga ccggcgccgc
      781 gatcctgacg acgctcgccg agttccgccg tccctcgttc gttccctccg cggtgggctt
      841 cggcttcggc acgatggaac ttccgtggcc gaacgcgctc cgcgtctgga tcggcgagac
      901 cgcctccacg agcgacgcgg gagatgacgg cgagctggtg ctcgagacga acatcgacga
      961 catgaacccc cagttcatcg aactggtgat ggagcgtctc tttgcggcgg gcgcgctcga
     1021 cgtctggctg accccgatca cgatgaagaa gggtcgcccg gcgacgacga tcagcgtact
     1081 tgccgcggcg agcaagcgtc acgatctcga gcggatcctg atcgagaaca cgaccacgct
     1141 cggcatccgt gccaccgcga tcgatcgcgt gaaggtaccg cgtgcgttcg agacggtcgc
     1201 aacgcgctgg ggtgacatcc cgctcaagct gaagatctgg aacggacggg tgatcgacat
     1261 ctcgccggaa tacgacgcct gcgtcgcgat cgcccgtcac cacgaccttc cggtgaagga
     1321 catctggaac gaggcgtacc ggctgggcga atcgctgatc ggccagcgcc gctagaggcg
     1381 ggacggacgg gcgggtcggt ccagaaccgc gatctggcgg cgacgatccg ccccgggtat
     1441 gaatgctgat atgcgacacc gcacacagcg cgttcgtccg agacgcggtc atacgtgggg
     1501 cgggactacg gaggtccccg accgccgatc atgaggtcgc agccggtgta tgtccgatcg
     1561 gtggccgggg gaatgtcccg cctcgcgccg gcacggagta tttctaccgc gtccgtttcg
     1621 aggcagggta tgaaacccaa tgcataaaca actgtcattt cgacttgtcg aaaaatcccc
     1681 gtgcgaaccc attcgacaag ctcaggtagg ctccggtgga ctccagcgcg tatattcccg
     1741 gcaccacgct ccccgggaaa cggatcggct gcgccgaccc agggcgacca caagggtcgc
     1801 gtctacggtt cagcgaacga tggtgatcgg aacgccatag tctggcaacg atcatatcg
//
LOCUS       DASTMD010000580         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2732776, whole genome shotgun sequence.
ACCESSION   DASTMD010000580 DASTMD010000000
VERSION     DASTMD010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2732776"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFQ54_08510"
     CDS             <1..269
                     /locus_tag="VFQ54_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165073.1"
                     /translation="PIKLLKPGAKEWEELPITFDFTTDSRGLGISDFARALKEGRTPR
                     ASGELSYHVLDTMHAILDSANDGKAVDVTSTFEKPALRAERFES"
     gene            514..975
                     /locus_tag="VFQ54_08515"
     CDS             514..975
                     /locus_tag="VFQ54_08515"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165074.1"
                     /translation="MKRLLLLVPFFLALIAFPVNAQGTTTLATSDNATLGTILTDANG
                     LTVYTFKKDTPGVSTCYDACATNWPPVTATEPLTLPAGVPGTLSLIDRTDGSKQLAYN
                     GQPLYTFAGDKAAGDTKGQAVGTLWYVVSPSAAAATPVASPVASPAATPIS"
     gene            1085..2236
                     /locus_tag="VFQ54_08520"
     CDS             1085..2236
                     /locus_tag="VFQ54_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0165075.1"
                     /translation="MPISRVSISNERTPLGMDHQARSGQPVGIGIIGAGVISQIYLEN
                     LTKRFPNVRVIGVADIVHAAAQARADQYGVRALTVSELLDEPAIEIVVNLTIPAAHAE
                     VGMAAVTAGKSVYNEKPLTGTRPEGRELLKLARENGVLVGGAPDTFLGAGLQTVRKLI
                     DDGEIGEIVAASGSMLSSGHESWHPNPGFYYQPGAGPMLDMGPYYVTALVSLLGPIVR
                     VGSSARASFPTRLITSQPHAGETITVNTPTHIAATLDFASGAIGTLTTTFDTHDTDHS
                     LLTIYGSEGSIRVPDPNTFGGPVKLLKAGAKEWREIPLVDGFPENSRGLGVADMARAL
                     RGGSPHRASGDLAYHVLDVMFATLESSELSNHCGIESTVARPAPLDPVF"
BASE COUNT          531 a          979 c          897 g          486 t
ORIGIN      
        1 gcccgatcaa gctgctcaag cctggtgcca aggagtggga agagctcccg atcacgttcg
       61 acttcacgac cgattcccgc ggtctcggca tctctgactt cgcgcgggcg ttgaaggaag
      121 gccgcacgcc gcgcgcgagc ggcgagctct cctaccacgt gctcgacacg atgcacgcga
      181 ttctcgactc cgccaacgac ggcaaggccg tcgacgtcac cagcaccttc gagaagccgg
      241 cgctgcgggc cgagcgcttc gaatcctgat cccctgtgtg gcggcgaaga gggaggtccc
      301 gaccgggacc tccctcttcg cgttttcgca tagccgcggt cgaattcggg cgaagatagc
      361 gggcgtcgat cggagatggc gggcatgcgg gtacacaatc tattcaaaaa cgcaccatag
      421 gtttgccgcg gcgtgtgtga tagggtgggg cggggaaacc ggcgtccctt ccttcgaagt
      481 ctcggatccc aattcaacac gaggtgactg cgcatgaaac gattgctgct gctcgtcccc
      541 ttcttcctcg ccctcatcgc attcccggtc aacgcccagg gaaccacgac actcgcaacc
      601 agcgacaacg cgacactcgg cacgatcctg accgatgcca acggtctcac ggtctacacc
      661 ttcaagaagg acacgccggg cgtcagcacc tgctacgacg cctgcgcgac gaactggccg
      721 ccggtcacgg cgaccgagcc gttgacgctt ccggctggag tgccgggcac gctgagcctg
      781 atcgatcgca ccgacggcag caagcagctc gcctacaacg gtcagccgct ctataccttc
      841 gcgggcgaca aggctgccgg cgatacgaag ggccaggctg tcggaaccct ctggtacgtc
      901 gtcagtcctt cggctgccgc cgcgactccc gtggcatcgc ccgtcgcctc tccggcggca
      961 acccctattt cctgattctt cttctgagac aacgagcgaa gcaccccctg ccgaagccgg
     1021 acagcgcggg gggtgttttg cgttcggacg ctggcggttg acgctggacc cggcgattgc
     1081 catcatgccg atatcgagag tcagcatcag caacgagagg acgccactcg gcatggatca
     1141 ccaggcacgc tcaggacaac cggtcgggat cggcatcatc ggggccgggg tcatctccca
     1201 gatctacctc gagaatctca cgaagcgctt cccgaacgtc cgcgttatcg gtgtcgcgga
     1261 catcgtccac gcggcggccc aagcccgcgc cgaccagtac ggcgtccgcg cgctgacggt
     1321 cagcgagctt ctcgacgaac ccgcgatcga gatcgtcgtc aacctgacga tcccggcggc
     1381 gcacgcggaa gtcgggatgg ccgcggtcac ggccgggaag tcggtctaca acgagaagcc
     1441 gctcaccggc acgcgcccgg aggggcgtga gcttctcaag ctcgcccggg agaacggcgt
     1501 cctcgtggga ggcgccccgg acaccttcct cggcgccggg ctgcagaccg tccgcaagct
     1561 gatcgacgac ggggagatcg gcgagatcgt agccgcgagc gggtccatgc tgagcagcgg
     1621 tcacgaatcg tggcatccga atccgggctt ctactatcag ccgggcgccg gtccgatgct
     1681 cgacatgggg ccatattacg tgaccgcgct cgtcagcttg ctcggcccga tcgtgcgcgt
     1741 cggatcctcc gcccgcgcca gcttcccgac gcgactgatc accagccagc cgcacgctgg
     1801 cgagacgatc accgtcaaca cgccgaccca catcgcggca acgctcgact tcgcgagcgg
     1861 cgcgatcggc acgctgacga cgaccttcga cacgcacgac accgatcaca gcctgctgac
     1921 gatctacggc tccgagggct cgattcgcgt gccggatccg aacaccttcg gtggcccggt
     1981 gaagctgctg aaggctgggg cgaaggaatg gcgggagatt cccctcgtgg acggattccc
     2041 cgagaattcc cgcggtctcg gtgtcgccga catggcgcgg gcgttgcgcg gcgggagccc
     2101 gcatcgtgcc agcggcgacc ttgcctatca cgtcctcgac gtgatgttcg cgacactgga
     2161 atcgagcgaa ttgagcaacc actgcgggat cgaaagcacc gtcgcccgcc cggccccgct
     2221 cgacccggtc ttctgatcga tcccggagcg atcgccatga tgggcgagac gggttgcgca
     2281 tatgttcgcg tcgcggctgg agataaactc cagccctacc aaccccggca ccaccaggcg
     2341 aacgtctggc aggcgattac gaatatccgt aggagcggac ctggtgtccg ccctgggctc
     2401 atcacccata cttccccgcc gcgggagtcg ccgaacgcag tcactgtgac aaagagccga
     2461 gccgtctagc ggcgacggcg ttggcacagc gttcgtgagt agggctgggc ttcaatgtcc
     2521 agccgtcagg cacagcctga catgatcccg cgaacggtag tctcgccgaa cgtggccgtc
     2581 ccggagtccg tacgggccac gtaccgtccg tgatctgcgt caagcgccgg ggagagcgtg
     2641 tgcgtccgaa catcatgccg ggcattgccc ggcagaagga cataagagtc cttccctacg
     2701 cacgaacgct tcggatcagc ccgagtatgg agagaagatc tatccgctct tccgcgcgtg
     2761 ctcgatcatg tatctcctga gcgcctcgtt catcgcggtc tggtatttgg gttgcgttcg
     2821 cttgaaccaa tccacgacat cagcatccaa ccgcaacgtc agttgctgct tgaccggccg
     2881 gtagaacttg ccc
//
LOCUS       DASTMD010000581         1631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_767866, whole genome shotgun sequence.
ACCESSION   DASTMD010000581 DASTMD010000000
VERSION     DASTMD010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767866"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFQ54_08525"
     CDS             <1..497
                     /locus_tag="VFQ54_08525"
                     /inference="COORDINATES: protein motif:HMM:NF040134.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165076.1"
                     /translation="MLILGPVIAFLGISGALLSRDLLDEADEQSSRVASTIHTLVVHA
                     VAFLGFSAIYLNKLDSWVSAPLAGILGGILILETLERGGAEPPQRIFYAVLGGWVLGT
                     ATVALDWWPTYGWTGGAVLLAIFYAAAGILLVQTQRSTVTRRDLIEFGGVGGVALLIL
                     ALMS"
     gene            550..777
                     /locus_tag="VFQ54_08530"
     CDS             550..777
                     /locus_tag="VFQ54_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165077.1"
                     /translation="MTSKKTDMPSNITNLAQRRAKKNQAKDDLFDLLSRIDELEDALE
                     IMDEHGVTTREELESLIAELERRAAELDEET"
     gene            804..>1631
                     /locus_tag="VFQ54_08535"
     CDS             804..>1631
                     /locus_tag="VFQ54_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0165078.1"
                     /translation="MDLFEANRQQLLASRAPLATRMRPRTLDELVGQEHILGPNTLLR
                     KAIEADRLSSLILWGPPGAGKTTLARIVAGTTRAAFDAVSAVTSGVADLRRAIADAQD
                     RLGMRGERTVLFIDEIHRFNKAQQDAILPHVEDGTVILIGATTENPSFEVNAPLLSRS
                     RVVTLHALSDENIKTIVRRALVDTERGLGKDELSIEDDGLDLLANMANGDARFALNTL
                     ELASAGVGDDRKITAPLVQEAAQRRAATYDKGGDDHYDAISALHKTLRGSDPDAALYW
                     "
BASE COUNT          292 a          547 c          505 g          287 t
ORIGIN      
        1 cgatgctgat cctgggaccg gtgatcgcct tcctcggcat ttccggcgcc ctcctttcgc
       61 gcgaccttct tgatgaagcc gacgagcaat cgagccgcgt tgcctcgacc atccataccc
      121 tggtcgtgca tgccgtcgcc ttcctcggtt tcagcgcgat ctatctgaac aagctcgatt
      181 cctgggtttc tgctcccctg gcgggaattc tcggcgggat cctgatcctc gagaccctgg
      241 agcgcggtgg cgccgagccg ccgcagcgga tcttctacgc cgtgctcggc ggctgggtcc
      301 tggggacggc gacggtcgcc ctcgactggt ggccgacgta tggctggacc ggcggcgcgg
      361 tgcttctcgc gatcttctat gccgccgcgg gcatcctgct cgtccagacg cagcgatcga
      421 ccgtcacgcg acgcgatctc atcgagttcg ggggtgttgg cggtgtcgca ctcctgatcc
      481 tcgcgctgat gtcctgatcc gaccaaccaa tgacatgatg aagtctgcga tcgaggaaac
      541 gattcgttca tgaccagcaa gaagacggat atgcccagca acatcaccaa cctcgcgcag
      601 cgccgggcga agaagaatca ggcaaaggac gatctcttcg acctgctatc ccggatcgac
      661 gagctggaag acgcgctcga gatcatggac gagcatggcg tgactacacg cgaggagctc
      721 gagtcgctca ttgcggagct tgagcgccgc gccgccgaac tcgacgagga aacgtagtcc
      781 cggccagaat cgtcaggcat cccatggatc tctttgaagc caaccgacag caactgctcg
      841 cgagccgggc gccactggcg acccggatgc gtccccgtac gctcgatgag ctcgtagggc
      901 aggaacacat cctcggaccg aacactctgc tccggaaagc gatcgaggcg gaccgccttt
      961 cctcgctgat tctctggggc cccccgggcg ccggcaagac gacccttgcc cggatcgtcg
     1021 ccggcactac gcgcgccgct ttcgatgccg tcagtgccgt gacctcgggt gtcgccgatc
     1081 tgcggcgggc gattgcggat gcgcaggatc gcctcgggat gcgcggggaa cggacagtcc
     1141 tcttcatcga cgagattcat cgcttcaaca aggcgcaaca ggacgcgatc ctcccccacg
     1201 tcgaggatgg cacggtcatc ctgatcggcg cgacgaccga gaatccctcg ttcgaggtga
     1261 acgccccact gctctcccgc tcgcgcgtcg tcaccctgca tgcgctctcc gacgagaaca
     1321 tcaagacgat cgttcggcgt gcgctcgtgg acaccgagcg gggcctgggc aaggacgaat
     1381 tgtcgatcga agacgacggg ctcgatctgc ttgcgaacat ggcgaatggc gacgcgcggt
     1441 tcgccctcaa tacactcgag cttgcctccg ccggtgttgg cgacgacagg aagatcacgg
     1501 cgccgctcgt ccaggaagcc gcgcagcgtc gcgcggcgac ctacgacaag ggaggcgacg
     1561 accactacga cgcgatctcg gcccttcaca agacgctccg cggctcggac ccggacgccg
     1621 cgctctactg g
//
LOCUS       DASTMD010000582         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2110446, whole genome shotgun sequence.
ACCESSION   DASTMD010000582 DASTMD010000000
VERSION     DASTMD010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110446"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..401
                     /locus_tag="VFQ54_08540"
     CDS             168..401
                     /locus_tag="VFQ54_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165079.1"
                     /translation="MPASDPGITLSPDDLRVLLRIADLTIEDERLPALLAEVNTQLRF
                     ASTLDRVLAGVAESDLAAFDPSWPSPDATEDAR"
     gene            398..1804
                     /locus_tag="VFQ54_08545"
     CDS             398..1804
                     /locus_tag="VFQ54_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU0165080.1"
                     /translation="MSFDLKTTTLQEAIAGLDNHDFSSVELIDATIARLQATEPEIHA
                     YVEIFADEARSQAQAADQNRAAERVTKPLLGIPIGVKDIFDVVGVPTRSGSKQRANVA
                     PAAKDAPVVARWREDGAIFTGKTVTQEYAAGVVSHPARNPWNPDHIPGGSSGGSAASV
                     AIGSCLGAMGSDTGGSIRIPAAVTGIVGLKPTFGRLTAKGVYPLSPSLDTVGPLARSV
                     ADAAILYLSMAGRTAEIDGVDSLLAPYGSKGLEGVRIGILSSFFTEMIQPDVATAFAD
                     AVQVLRDLGATIVETDWSEATTARAIAILISRIESGTIHHDNLRNHPELILADARIRF
                     EAGQLLPANLYLRARMAREVIRDSIARVYAGNRLDAIAAPTLAATAPRADNLIVEFPE
                     GKEVVGFALTRYAQPWNATGQPVITVPSGADGNGLPIGLAIVGRPDDEIGISRIAAQY
                     EQATGWFQANAAKMLAGR"
     gene            1829..2713
                     /locus_tag="VFQ54_08550"
     CDS             1829..2713
                     /locus_tag="VFQ54_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU0165081.1"
                     /translation="MTSQLAPLVTITGAGGNGGQAIARAAAAGFRLRLADMAQPPADI
                     LALGEFMRCDTRTVSDVQRAIEGADAVIHLAAWHSAHRPPVSDATIFAVNIDGTFNVF
                     EACRQYGVKSIVYASSMAYGWGNVYGLTKVLGEEMCRTYHEITGASIAMLRYHEFVPA
                     PYLTFGPRLLRNGVDRADVASATVAALQAAIARKFGLFMTIVHTAHGIPDDVLADFPT
                     LGPDWLESQVPGAKELIAKYALPLPQTVEQHDLSEAAAVLGWKPSVGFLDFLRDLKER
                     DANGEDVSAIFVPGTLHP"
BASE COUNT          522 a          965 c          921 g          465 t
ORIGIN      
        1 cctcgacagg ctcgggagga cgtgtcatgg gggtcgtcgt acggaaggga gtgcatatcc
       61 gtggcgaatc gacgagggtc gtgctaccgt gcggaaatct gacccggcgg tggccgccgg
      121 gccgaaccgc tcgccgttcg cctgacacgc ccaaggatga ccgcccgatg ccagcttccg
      181 accccgggat aacgctttcc ccggacgatc tccgcgtcct cctccgcatc gccgacctga
      241 cgatcgagga cgagcgcctc ccggcgctcc tcgccgaagt gaatacccag ctccggttcg
      301 cgagcaccct cgatcgcgtg ctcgcaggag tcgccgagtc cgatctggcg gccttcgatc
      361 cctcctggcc atcgcccgat gccacggagg acgcacgatg agcttcgacc tgaagaccac
      421 gacgctccag gaagcgatcg ccggtctcga caaccacgac ttcagctccg tcgaattgat
      481 cgacgcgacg atcgcgcgac tccaggcaac cgaaccggag atccatgcct acgtcgagat
      541 cttcgccgat gaggcgcgat cccaggcgca ggcagccgat cagaacaggg cggcggaacg
      601 cgtgacgaag ccgctgctcg ggattccgat cggggtcaag gacatcttcg atgtcgtcgg
      661 cgtcccgacg cgtagcgggt cgaaacagcg cgcgaacgtc gcaccggcgg caaaggacgc
      721 acccgtcgtc gcccgctggc gtgaggatgg cgcgatcttc accggcaaga cggtaacgca
      781 ggaatatgcc gccggggtgg tcagccatcc ggcccggaat ccgtggaacc cggatcacat
      841 cccgggcggc agttccggcg gctccgcggc gtcggtcgcg atcggcagtt gcctcggcgc
      901 gatgggatcc gacaccgggg gcagtatccg cattcccgcc gccgtgaccg ggatcgtcgg
      961 cctgaagcca accttcggcc ggctgaccgc aaagggcgtc tacccgctct cgccttcgct
     1021 cgataccgtt ggaccgctcg cgcgctccgt tgccgacgcc gcgatcctct atctctcgat
     1081 ggccggtcgc accgcagaga tcgacggcgt cgactcgctc ctcgctcctt atggaagcaa
     1141 ggggctcgaa ggcgtccgga tcggcatcct gagttccttc ttcacggaga tgatccagcc
     1201 cgacgtcgcg acggccttcg cggacgccgt ccaggtcctg cgcgacctcg gcgcgacgat
     1261 cgtcgagacc gactggtcgg aagcaacgac cgcccgcgcg atcgcgatcc tgatcagccg
     1321 gatcgaatcg ggaacgatcc accacgacaa cctgcgcaat catcccgagc tgattctcgc
     1381 cgacgcccgc atccgtttcg aagctggtca actgctcccg gcgaatcttt acctgcgagc
     1441 tcgaatggcg cgcgaggtga tccgcgacag cattgcccgt gtctacgccg ggaatcggct
     1501 cgatgcgatt gcggcgccaa ccctggccgc gactgctcca cgcgcggaca acctgatcgt
     1561 cgaattcccg gaggggaagg aagtcgtcgg cttcgcgctg acacgatacg cgcagccgtg
     1621 gaatgcgacg ggccagcccg tgatcaccgt gccgagcggt gcggacggaa acggcctgcc
     1681 gatcggtctc gcgatcgttg gccgcccgga cgacgagatc gggatcagcc ggatcgcagc
     1741 gcaatacgag caggcaacgg gctggttcca ggcaaacgcg gcgaagatgc tcgccggtcg
     1801 ctaactggcg aagagaaaga ggtcaacgat gacatcgcaa cttgctcccc tcgtcacgat
     1861 caccggcgcc ggtggcaacg gcggtcaggc gatcgctcgt gccgccgccg cgggatttcg
     1921 attgcgcctg gcggatatgg cccagccgcc ggccgacatc ctcgcgctcg gcgaattcat
     1981 gcgctgcgat acccgcaccg tgagtgacgt ccagcgcgcg atcgagggtg cggatgcggt
     2041 gatccacctc gccgcctggc acagcgcgca tcgaccgccg gtcagcgacg cgacgatctt
     2101 cgccgtcaac atcgatggca ccttcaatgt cttcgaagcc tgccggcagt acggcgtgaa
     2161 gtcgatcgtc tacgcctcgt cgatggcgta tggctggggg aatgtctacg gcctgacgaa
     2221 ggtcctcggc gaggagatgt gccggaccta tcacgagatc accggcgcct cgatcgcgat
     2281 gctccgctac cacgaattcg tgccggcgcc gtacctgacg ttcggtccgc gcctgctccg
     2341 caacggggtc gaccgcgcgg acgtcgcgtc ggccaccgtc gcggccctgc aggcggcgat
     2401 cgcccggaag ttcgggctct tcatgacgat cgtccatacc gcgcacggga ttccggacga
     2461 cgtgctcgcg gacttcccga cgcttgggcc cgactggctg gaatcacagg tccccggggc
     2521 gaaggaactg atcgcgaaat acgcgcttcc gctcccacag acggtcgagc agcacgatct
     2581 ctccgaagcg gcggcggttc tgggctggaa accatccgtc ggattcctcg acttcctgcg
     2641 cgacctgaag gaacgcgatg ccaacggcga ggatgtgagc gcgatcttcg tcccgggaac
     2701 gctacatccg tagacgaatc ggtaagggcg gtgagggcgg ggttcaaacg agcattgccg
     2761 atgtgatgtc aaagtatgtc caactttgct atgatcgtcg ccaggaggac aagcaatgaa
     2821 cgttagcctg acaccacaac tcgagcagat cgttcgcaag aaggtcgaga ccg
//
LOCUS       DASTMD010000583         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2913001, whole genome shotgun sequence.
ACCESSION   DASTMD010000583 DASTMD010000000
VERSION     DASTMD010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2913001"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFQ54_08555"
     CDS             complement(<1..348)
                     /locus_tag="VFQ54_08555"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEU0165082.1"
                     /translation="MKSVARHWKTRARQLKQLVMTLYFASRDPRTPPIARIVGICVVA
                     YGLSPIDLIPDFIPVLGLLDDLILLPLGIALAIRLIPRDVWTDAQAKSAEISRSDRPT
                     NWIAAVIIVFVWIV"
     gene            656..1462
                     /locus_tag="VFQ54_08560"
     CDS             656..1462
                     /locus_tag="VFQ54_08560"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU0165083.1"
                     /translation="MHDQLIAIILGIVEGLTEFVPVSSTGHLIVAGHLLGFTGDRADT
                     FEVFIQLGAILAVTLIFRERIMSILRFGPGEGAQGKSGFILVAITTIPGLIAGALLNS
                     PIKDHLFSPGTVAVGWAIGGILIILVERNLPPVRKGGLDKIRWRDALIIGICQCAALW
                     PGVSRSASTMSAGMLLGIDRKTAAEYSFLAALPLIAAASFFDLVSNRNAIHASDIPMF
                     ATGFIVSFIVGWFAVRYFLRLLSTWTLVPFGWYRIAATVVLVFLIAINQV"
     gene            complement(1562..2689)
                     /locus_tag="VFQ54_08565"
     CDS             complement(1562..2689)
                     /locus_tag="VFQ54_08565"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0165084.1"
                     /translation="MSETSPIRLGIIGTGLAVQKLHWPALARLKDQFVVTAFTNRSRA
                     KAEHFADYSGTPMSLFVEETAQLLARDDVDAVLISLPIPLNLPTTLAALEAGKNVICE
                     KPAGANEQEARAFVELGNRFPDRTILIAENWFYRDDLRYARSLLDAGAIGDMHLVSWR
                     TVSQLIPREGEFSSTPWRHHAEYDGGPHLDAGVHHTAQLRLLCGDVSRLHGETQDTNK
                     THVGPSDLVLSFRLVNGAVGNYTASWPDIPVPAGDNDMHIYGTDGQMAIGRDGITVTR
                     ADGANVRYRIQQRDGGYYNEFRNFHEAVVYGAPLVGTITQSFRNMELVLKSLESARKG
                     QVISLSELPESLSATAVPLWKPYGQEELYGDLDVTVTQEAL"
BASE COUNT          510 a          919 c          894 g          563 t
ORIGIN      
        1 cacgatccac acgaacacga tgatgactgc cgctatccag ttcgtcgggc gatcgcttcg
       61 cgagatctcc gcactctttg cctgggcatc ggtccacaca tcgcgcggga tgagccggat
      121 cgccagcgcg atccccagcg gcagcagaat caggtcgtcg agcagcccga gaaccgggat
      181 gaaatccggg atgaggtcga tcggactcag cccataggcg acgacgcaga ttcccacgat
      241 cctggcgatc ggtggcgttc gcggatcccg gcttgcgaaa tacagagtca tcacgagctg
      301 tttcaactgc cgcgcgcgcg ttttccagtg acgtgccacc gacttcattc catctcctcg
      361 tcgtccggct ccggccagga tcgatactcg gcctacgacg ctgagcccca gcgtgagcac
      421 catacaacgc ccggcgatga tgaagaatga tttcattttc tgcgactata ctcatggccg
      481 ctgccccggg tgggcatgaa gctgaacggc ccggcgccgg ggattcgcgc aatttccggc
      541 gggctgccca gtccaggaat ctctcaacgt ccgaatgcgc cggtgacggc atcttgatct
      601 cgaccgggga cctcacgcgc tgcgaaaccc caaggtcgta gaggagttct gctgcgtgca
      661 cgatcagttg atcgcgatca ttctcggtat cgtcgagggc ctcaccgagt tcgtccccgt
      721 ctcctcgaca ggacatctga ttgtcgccgg tcacctcctg ggattcaccg gtgaccgtgc
      781 cgataccttc gaagtcttca tccagcttgg cgccattctc gcggtaaccc tgatcttccg
      841 cgagcggatc atgtcgattc tcaggttcgg gccgggcgag ggcgcccagg ggaagtcggg
      901 cttcatcctc gtggcaatca cgacgattcc gggtctgatc gccggcgcgc tcctgaacag
      961 tcccatcaag gaccacctct tctcgcccgg cactgtcgcg gtaggatggg cgatcggcgg
     1021 catcctgatc atcctggtcg agaggaatct gccgccggtc cgcaaggggg ggctggacaa
     1081 gatccgctgg cgcgacgcgc tcatcatcgg catctgccaa tgcgccgcgc tctggcccgg
     1141 tgtctcgcga tcggcatcga cgatgagtgc ggggatgctg ctcggcatcg accgcaagac
     1201 cgccgcggag tattccttcc tcgccgcgct gccactgatc gccgccgcaa gcttcttcga
     1261 tctggtcagc aatcgaaacg ccatccacgc ctcggacatc ccgatgttcg cgaccggctt
     1321 catcgtgtcg ttcatcgtcg ggtggttcgc cgtgcgctac tttctccggc tcctgtcgac
     1381 gtggaccctg gtcccgttcg gctggtatcg catcgccgcg accgtcgtcc tcgtgtttct
     1441 gatcgcgatc aatcaggtct gagccagtcg cggctgtcgc gttgccgaga ttcacgtaga
     1501 tacacgatcg cacaaagggg cgccggagga agatcctccg gcgccccttt tgtgtgttgg
     1561 gttagagagc ttcctgcgtc accgtgacat cgaggtcgcc gtagagctct tcctgcccat
     1621 acggcttcca gagtggcacg gcggtcgcgg aaagcgactc cggcagttca ctgagggaga
     1681 tcacctgtcc cttgcgggca gactcgaggc tcttcagcac gagctccatg ttccggaagc
     1741 tctgggtaat cgtcccgacc agcggcgcac catagacgac cgcttcgtgg aagttccgga
     1801 actcgttgta gtaaccgccg tctcgctgct gaatccggta gcggacattg gcgccgtcgg
     1861 cgcgggtgac cgtgatccca tcgcgaccga tcgccatctg cccatccgtg ccgtagatgt
     1921 gcatgtcgtt gtcgccggcc ggaaccggga tgtccggcca actcgccgtg tagttgccga
     1981 cggcgccgtt gacgaggcgg aagctcagga cgaggtcgga tgggccgaca tgcgtcttgt
     2041 tcgtatcctg ggtctcgcca tgaagccggc tgacgtcgcc gcaaagaagg cgaagctgcg
     2101 cggtgtggtg aacgcccgcg tcgaggtgcg ggccgccgtc gtactcggcg tgatgccgcc
     2161 acggcgtcga ggagaactcg ccctcacgcg gaatgagctg gctcaccgta cgccaggaga
     2221 cgagatgcat gtcgccgatc gcgcccgcgt cgagcagcga ccgcgcgtag cggagatcgt
     2281 cgcgatagaa ccagttctcg gcgatcagga tcgtgcgatc cgggaagcga ttgccaagct
     2341 cgacgaaggc gcgcgcttcc tgctcgttgg ctcccgccgg cttctcgcag atcacgttct
     2401 tgccggcctc gagtgccgcg agcgtcgtcg gcaggttgag tggaatcggg agggaaatga
     2461 ggacggcatc cacgtcatcg cgagccagca gttgcgcggt ctcctcgacg aagagggaca
     2521 tcggggtgcc gctgtagtcc gcgaagtgct cggccttggc gcgcgaacgg ttggtaaagg
     2581 cggtgacgac gaactggtct ttcaatcgag cgagtgctgg ccaatgcagc ttctggaccg
     2641 cgaggccggt gccgatgatg cccagccgga tcggtgacgt ttccgacata ttctcccttg
     2701 ccatttcctg acgatgcgat cagcgccaga gtccgactct cgacgacagg catgacgcgc
     2761 aacgcgccag caggcatcga caccgtatcg atccgccgga tgcgtcacac gcgtcacaca
     2821 aacgctgaca acccttgcgc aatcttatcg tcatatcacg aaattggata tccagggaga
     2881 gacaac
//
LOCUS       DASTMD010000584         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1842086, whole genome shotgun sequence.
ACCESSION   DASTMD010000584 DASTMD010000000
VERSION     DASTMD010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842086"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..1725)
                     /locus_tag="VFQ54_08570"
     CDS             complement(346..1725)
                     /locus_tag="VFQ54_08570"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEU0165085.1"
                     /translation="MTSVSARGRLTVDELIARVRSGAIDTVITAICDMQGRLVGKRVT
                     GEFFIDHALEHGTHFCTYLLGTDMEMETPDGYAAMDWASGYGDYLARPDWGTLRVIPW
                     LEKTALVLCDAVDETTGATIDFAPRAILKRQIERLAAHGLAPMMATELEFYVLKESYD
                     EARAKQFVGLTPFGWYNEDYQLLQASKAEPLYRRLRNEMTAAGIPIEFSKGEAAPGQH
                     EINIHYADALESADRAALFKHGAKEIAYQAGNAVTFMSKPHHEWTGSSGHVHVSLVDA
                     DSGKNVFATESGEMSETMRWFLGGLTAGARDLSILLASTVNAYKRFALASWAPVHLVW
                     GRDNRTCAFRVVGRGSGLRIENRLPGADANPYLSFAAVLAAGLDGIERQLDPGEEFRG
                     NGYTATDLPRIPKTLYRAIDAWEASDLARRAFGEDVHAHYLNMATVEQEAYDRVVTDW
                     ERERYLERG"
     gene            1918..>2152
                     /locus_tag="VFQ54_08575"
     CDS             1918..>2152
                     /locus_tag="VFQ54_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU0165086.1"
                     /translation="MSESSDTLLAKRLEAAKAHFSGRFSAAARNLTTGEEILVDPHRQ
                     YPTASTFKVPIMVTVFKQIEEGKYKIHDRLTLKE"
BASE COUNT          388 a          695 c          674 g          395 t
ORIGIN      
        1 gacgtatcct ggatggcgtt gcacggcaag acacgtgatc tcaacgtgat ccctcgacgg
       61 gctcgggagg acgtatcctg ggtggcgttg catggcaaaa cacgtggtat caacgtgatc
      121 cctcgacagg ctcgggaaga cgtatcccgg gtggtgtcgt gcagtaccgc gactcgcgcc
      181 ttgcgggggc tacggcgtcg ccgcggagcg tttctgacgc gcgatgctcc acgacgaccg
      241 gggtttgtag ggctggagct cgtctctagc cgcaagagcg aacgtcccat tcaacgtgtg
      301 tgcggaaacg gcgacaagcg ccgtggggac cacggagacg cctcgctacc cgcgttcgag
      361 gtaacgctca cgctcccaat cggtgacgac ccggtcgtac gcttcctgct cgaccgtcgc
      421 catgttcagg taatgggcgt gaacgtcctc accgaaggcc cgacgggcga gatcactcgc
      481 ctcccaggca tcgatcgccc ggtagagcgt cttcgggatt cgcggcagat cggttgcggt
      541 atagccgttt ccccggaatt cctcacccgg gtcgagttga cgctcgatcc cgtcgagccc
      601 cgccgccagc accgccgcga agctcaggta cgggttggca tcggcgcccg gcaggcgatt
      661 ctcgattcgc aggcccgatc cacgcccgac gacgcggaag gcacaggtcc ggttgtcccg
      721 cccccagacg agatgcactg gcgcccacga cgccagcgcg aagcgcttgt aggcattcac
      781 cgtcgaggcg agaaggatcg agaggtcgcg cgccccggcg gtgaggccgc caaggaacca
      841 gcgcatcgtc tctgacatct cgccggattc cgttgcgaat acgttcttgc cgctgtcggc
      901 gtcgacgagg ctgacgtgaa cgtggccgct cgatcccgtc cattcgtgat gcggtttcga
      961 catgaaggtg accgcgtttc cggcctggta cgcgatctcc ttcgcgccgt gcttgaagag
     1021 cgccgcccga tcggccgatt ccagcgcatc ggcgtagtgg atgttgatct cgtgttgacc
     1081 gggcgccgcc tcgcccttcg agaactcgat cggaatcccg gcggccgtca tctcgtttcg
     1141 caaccggcgg tagagcggct ccgccttcga ggcctgcagg agctggtaat cctcgttgta
     1201 ccagccgaac ggggtcagcc cgacgaattg cttcgcccgc gcctcgtcgt acgattcctt
     1261 gaggacgtag aactcgagct cggtcgccat catcggcgcg agtccatgcg cggcgagacg
     1321 ttcgatctgc cgcttcagga tcgcccgcgg cgcgaagtcg atcgtcgcgc cggtcgtctc
     1381 gtcgacggcg tcgcagagca cgagcgccgt cttctccagc cacgggatca cgcggagcgt
     1441 cccccagtcg ggacgcgcga ggtagtcacc gtacccgctt gcccaatcca tcgccgcgta
     1501 gccgtccggc gtctccatct ccatatcggt tccgaggaga taggtgcaga agtgcgtgcc
     1561 gtgctcgagc gcgtgatcga tgaagaattc ccccgtcacc cgcttcccga cgagacgccc
     1621 ctgcatgtcg cagatcgcgg tgatcacggt gtcgatcgcg ccggaacgga cacgagcgat
     1681 gagttcatca acggtcaggc ggcccctggc ggaaacagaa gtcatgcgtg cggttctcca
     1741 tcgttcatcg gtagcggacg cagggttcac gatggccgca ttgtacttcc ggtccaatcg
     1801 tcaacgattg cgcaaacagg tgcgttcaga ttccccattg cggagtatgg ctgggcgttg
     1861 atagcatcgc gcacaggtct tgattggcac atctgaacat gtccgggaga agcacgcatg
     1921 agtgagtcgt ccgataccct gctcgcgaaa cgtctggaag ccgcaaaggc acacttttcc
     1981 ggacgcttct cggcggcggc ccgcaatctc acgacaggcg aggaaatcct cgtcgatccc
     2041 caccgccagt acccgaccgc cagcaccttc aaggtgccga tcatggtcac cgtcttcaag
     2101 cagatcgagg aaggaaagta caagattcac gaccgcctga cgctgaagga aa
//
LOCUS       DASTMD010000585         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2110717, whole genome shotgun sequence.
ACCESSION   DASTMD010000585 DASTMD010000000
VERSION     DASTMD010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110717"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFQ54_08580"
     CDS             complement(<1..899)
                     /locus_tag="VFQ54_08580"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU0165087.1"
                     /translation="MTHVPGGILYPDGQVGPGLEAPSPGHSDIKAVEKMDRNELDAMR
                     QRIAASVRAHAMLAEAGRPDIDPCIVAEEAMPGYLFGELNPSEQRWLIAHIEGCTWCS
                     RIFASFGELNAALDEIDAEVGARTPPSVAGRLGLKEACYGQMETPVGPLLVAVSDGKL
                     CATSWIRNGTREAAIRDLEARGFLAVANQDAVAPVIDQLNRYFEGAIRGFDIEIDLSG
                     VSEFTHKALIGIRDIAYGDVKTYGDVARKIGQPGATQAVGNAMGRNPIPIVIPCHRVV
                     RSDGSMGHYTGGADIKEALLAIEG"
     gene            complement(901..1539)
                     /locus_tag="VFQ54_08585"
     CDS             complement(901..1539)
                     /locus_tag="VFQ54_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0165088.1"
                     /translation="MMSAVLMDEPKSFPKTGVTPFAVEFEGDIEDVAIGTFDGSTTAV
                     EESISFSSVIERFGTEIYRFSWHLARNQADADDLYQETLFKAFRAFDRLPADANHRAW
                     LYRIASNTFISDRRKRSREHALTDVIEQTATTGAPDHARGLDARDLLGDVERFVRQLP
                     PKQRIALVMRKYQGAEYAQIAEALDCSEVAARANVHEALRKLKSQFAHRLES"
     gene            1960..>2334
                     /locus_tag="VFQ54_08590"
     CDS             1960..>2334
                     /locus_tag="VFQ54_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165089.1"
                     /translation="MTETVAYPGDKQAIYTRILAAIDRETARINAVADEIHANPELGM
                     EEFKASALMTETISRYGYEVERGTGGIATAFKAIRGGRGSGPAIAFLAEYDALPGLGH
                     GCGHNLIASSNLAAAIGLGEVIA"
BASE COUNT          436 a          772 c          682 g          444 t
ORIGIN      
        1 ccctcgatcg cgagcagcgc ttccttgata tccgcaccgc ccgtgtaatg ccccatgctg
       61 ccatccgaac gcaccacacg gtgacatggg atcacgatcg ggatcggatt ccgccccatc
      121 gcgttgccga ccgcctgtgt cgcccccggc tgcccgatct ttcgcgccac gtccccatac
      181 gtcttgacat cgccgtacgc aatatcacgg attccaatca acgccttgtg cgtaaactcg
      241 ctgacgccgg aaagatcgat ctcgatgtca aatcccctga tcgccccttc aaaatagcga
      301 ttcaactggt cgatgaccgg cgccacggcg tcctgattcg cgaccgcgag gaatcctcgc
      361 gcctcgagat ccctgatcgc cgcttccctc gtcccattgc ggatccagga tgtcgcgcag
      421 agcttcccgt ccgagacggc gaccagcagc ggcccgaccg gcgtctccat ctgcccgtaa
      481 caggcttcct tcaggccaag cctgccggcc accgatggcg gagtcctcgc gccaacctcg
      541 gcatcgattt cgtcgagcgc cgcgttcagc tccccgaagc ttgcgaagat cctgctgcac
      601 caggtgcaac cttcgatgtg cgcaatcagc catcgctgct cggatgggtt caactccccg
      661 aacagataac cgggcatcgc ctcctcggcg acgatacagg ggtcgatgtc cggtcggccc
      721 gcctctgcca gcatcgcgtg cgcgcgaacg ctggcggcga tccgttgccg catggcgtcc
      781 aactcgttcc tgtccatctt ctcgaccgcc ttgatatcgg aatggcccgg ggacggcgct
      841 tccagtcccg gtccgacctg gccgtcagga tacaggatgc cccccggcac gtgagtcacg
      901 tcacgactcc agtctgtgcg caaactgcga cttgagtttc cggagcgcct cgtggacatt
      961 ggcgcgggcg gcaacctcgg aacaatcaag cgcctcggcg atctgggcgt actcggcacc
     1021 ctgatacttc ctcatcacga gcgcgatccg ctgtttgggc ggaagttgcc gcacgaacct
     1081 ctcgacatcc ccgagcagat cccgcgcatc gaggccgcgc gcgtgatccg gggccccggt
     1141 cgtcgccgtc tgctcgatca cgtcggtcag cgcgtgctca cgggagcgct tgcggcgatc
     1201 gctgatgaac gtattggacg cgattcggta aagccaggcc cgatggttgg catccgccgg
     1261 caggcgatcg aaggcacgaa atgccttgaa gagcgtttcc tggtagagat cgtcggcatc
     1321 ggcctgattc cgggcgagat gccaggagaa acgatagatc tccgtgccga accgctcgat
     1381 gaccgaactg aaagaaatcg attcctcgac tgcggtcgta cttccatcaa aggtcccgat
     1441 cgccacgtcc tcgatatcgc cctcgaattc gacggcgaag ggcgtcaccc cggtcttggg
     1501 gaagctcttg ggttcatcca tcaggactgc ggacatcatg tgcgtcgttc gccttcattc
     1561 ttgtcatcgt ccgcttcgat cgttgccaat cgatgccggt ccttcctgta acgcgcgttc
     1621 gttgcgcacg tgagacagat aggcgaagat cgatgccgac atgagtcgga agaagcgcgt
     1681 tcgcgcggtt cgcgtatctc ttcatgattc aggtagctca ccccgggatc cggatcacca
     1741 caccggtccc gcatctgacc gtgagttcat gataggcggc aatcggcgca gactcgatac
     1801 gcaaaatcgc gtatttcgtg caacgcgcca cgtacgcaat cgaaagatct ccagagggcg
     1861 atcttcccgt gacgtccgtg gtcgccgtcg ataatcccgg attgtgtgcc cggaaaagcc
     1921 cactgtcgcg gtcagcgaac cgcggaggag aaatgcggaa tgacggagac tgtcgcgtac
     1981 cccggggaca aacaggcgat ctacactcgg attctggcgg cgatcgatcg cgagaccgcg
     2041 cgaatcaacg cggtcgcgga tgagattcac gccaatccgg aactcgggat ggaagagttc
     2101 aaggcgtcgg cgctcatgac cgagacgatc tcccgctacg gatatgaggt cgagcgcgga
     2161 accgggggca tcgcgaccgc attcaaggcg attcgcggcg gcaggggaag tggtcccgcc
     2221 atcgcctttc tcgccgagta cgacgcgctt cccggtctcg gtcacggctg cgggcacaac
     2281 ctcatcgcga gttccaatct cgccgccgcg atcggactgg gcgaggtgat cgcc
//
LOCUS       DASTMD010000586         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_859401, whole genome shotgun sequence.
ACCESSION   DASTMD010000586 DASTMD010000000
VERSION     DASTMD010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859401"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFQ54_08595"
     CDS             <1..315
                     /locus_tag="VFQ54_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165090.1"
                     /translation="ANVLRSPGAVRVASPGVLSGKQVPLMSNPQNSLRFYIDQADDKA
                     VVRAVPVKPVVATPRVATVEMPGEAVEVKQDATPEIRGIRRGRVATERVSRLVHRGRK
                     HS"
     gene            354..1763
                     /gene="mgtE"
                     /locus_tag="VFQ54_08600"
     CDS             354..1763
                     /gene="mgtE"
                     /locus_tag="VFQ54_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU0165091.1"
                     /translation="MSSPVSEIDPQQFPASDSEESLLDQIRAAVAAGDVERARSRANR
                     MLEDDALALLEDLTPEELGRLFVFLGDETLAVLLSRLDDRDAAHILTRMSAAQAADIL
                     EQIEPDDATDIFAEVEQANPEVAGTILIEMDPDEAAEIRDLMAYPPDTAGGIMTPSFV
                     AVFPDLRADQTINALRRVAEQTETINYVYVVDRNEKLLGVLSLHRLVLSRPETPVTDL
                     MMPEIISVRVDADQEEAAHILTERDLLALPVVDDDGRLLGIITADDVHDILEEEATED
                     IARLGGSQPLDVPYLRASPLMLFRKRIVWLFVLFVAQFMTVSIQEHYESILAQVTILA
                     VFVPILIGTGGNVGSQTVSTIIRAMALGEVHPRHILRIIGKEMLTGLSLGVVMGILMF
                     ARAEFTGDGSFDVALTVGLTILALCTWAATVGAVLPIVLSKLRVDPAVVSAPFISSFV
                     DSTGLIIYFTLAGILLNVS"
BASE COUNT          332 a          655 c          564 g          366 t
ORIGIN      
        1 gccaatgttc ttcgctctcc tggtgctgtg cgagtcgcca gtccaggggt gttgagcggg
       61 aagcaggtgc cattgatgag caatcctcag aactctctgc gcttctacat tgaccaggct
      121 gatgacaagg ccgttgttcg ggccgtgccc gtcaagccag tcgtcgctac gccgcgcgtc
      181 gccaccgtgg agatgccggg cgaggctgtc gaggtcaagc aggacgcaac ccccgagatt
      241 cgcggcattc gccgcggacg tgtcgctacc gagcgggtca gccggctcgt tcaccgcggc
      301 aggaagcact cttgattacc ccgggatcgg tcttcaagaa acggtcctag gcaatgtcga
      361 gcccagtcag cgagatcgat ccccagcaat ttccagcatc cgattccgag gaatcactcc
      421 tcgaccagat tcgcgcggct gtcgccgccg gtgacgtcga gcgtgctcga tctcgcgcga
      481 atcggatgct ggaggatgac gccctcgccc tcctcgaaga cctcacgccg gaggagctcg
      541 gtcgtctctt cgtctttctc ggcgacgaga cgctcgccgt gctcctgtcc cgtctcgacg
      601 atcgcgacgc cgctcacatc ctgacccgga tgtccgccgc gcaggccgcc gacatcctcg
      661 agcagatcga accggacgat gcgaccgaca tcttcgccga ggtcgagcag gccaatcccg
      721 aggtcgccgg gacgatcctg atcgagatgg atccggacga agccgccgag atccgcgatc
      781 tgatggccta tccgccggac accgccggcg gcatcatgac gccctcgttc gtcgccgtct
      841 ttcccgacct ccgcgccgac cagacgatca acgcgctgcg cagggtcgcc gagcagaccg
      901 agacgatcaa ctacgtctat gtcgtcgatc gcaacgagaa gctcctcggc gtgctctcgc
      961 ttcaccggct cgtcctgagc cgaccggaga caccggtcac cgacctgatg atgccggaga
     1021 tcattagcgt ccgcgtcgat gccgaccagg aagaagccgc ccacatcctg acggaacgcg
     1081 atctcctcgc gctccccgtc gtcgacgacg atggtcgcct gctcggcatc atcaccgctg
     1141 acgacgtcca cgacatcctt gaagaggaag cgacggaaga catcgctcgt ctcggtggtt
     1201 cgcagccgct cgatgtcccc tatcttcgcg ccagcccgtt gatgctcttc cggaagcgca
     1261 tcgtctggct cttcgttctc ttcgtcgccc agttcatgac cgtctcgatt caggaacact
     1321 atgagtcgat ccttgcccag gtgacgatcc tcgccgtctt cgtgccgatc ctgatcggaa
     1381 ccggcggcaa tgtcggctcg cagaccgtct cgacgatcat ccgcgcgatg gcgctcggcg
     1441 aggtccatcc ccgccacatt ctccgcatca tcggcaagga aatgctgacg ggtctctccc
     1501 tcggtgtcgt gatgggcatc ctgatgttcg cccgcgccga attcaccggc gacggcagct
     1561 tcgacgtcgc gctgaccgtc ggcctgacga tcctcgcgct ctgcacgtgg gcggcaacgg
     1621 tcggggcggt gcttccgatc gtcctcagca agctccgggt cgatcccgcg gtcgtctcgg
     1681 cgccgttcat cagcagcttc gtcgacagca ccgggttgat catctacttc accctggcgg
     1741 gaatcctcct caacgtgagc tagaacgcgg ggcaggacaa ggtgggacgt agaagcccca
     1801 ccctagggac gaccggcata cgtacagcgt ccgttcgtag ggcgggactt ctatgtcccg
     1861 cctttgatgt cacgctatca ggcccaagct ttggtagatg ccaccataga gcggctg
//
LOCUS       DASTMD010000587         5175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2555288, whole genome shotgun sequence.
ACCESSION   DASTMD010000587 DASTMD010000000
VERSION     DASTMD010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2555288"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFQ54_08605"
     CDS             <1..297
                     /locus_tag="VFQ54_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU0165092.1"
                     /translation="TGSRMDDVIYEEFKGTGNMELHLDRKLAERRIYPAIDIQRSSTR
                     REELLLDDFTLRQVWTMRRMVSMLGGNEGTELMLSRLSKTQKNSEFLETLTKDV"
     gene            complement(372..593)
                     /locus_tag="VFQ54_08610"
     CDS             complement(372..593)
                     /locus_tag="VFQ54_08610"
                     /inference="COORDINATES: protein motif:HMM:NF021634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0165093.1"
                     /translation="MTLFNDEALRVIPSVNYSHLRDTAARRIPRDPNDWAPIALALHL
                     GAGILTNDCDFLGCGCPTWTFQTLHEELG"
     gene            complement(796..1149)
                     /locus_tag="VFQ54_08615"
     CDS             complement(796..1149)
                     /locus_tag="VFQ54_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165094.1"
                     /translation="MAIKDRVEAAASLAEFRRAAAQYLAASDVENVGIREFRDHATRY
                     FASSKPVAISRHGKIVGFYVPVERDKEDMKRALDDLGRTVEAILERTGMTEDEFADLF
                     DLNKPLPPEYEDDGR"
     gene            complement(1278..2255)
                     /locus_tag="VFQ54_08620"
     CDS             complement(1278..2255)
                     /locus_tag="VFQ54_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEU0165095.1"
                     /translation="MSNVGVVTASANGRVGIEAAIEVLKAGGSALDAVIAGTRLVEAN
                     PEDHSVGFSGLPNLLGEVELDASVMEGTGLRAGSVGALQGYQDAVDLARHVMEELPHV
                     LIAGQGSARFAAEMGLTSSNLLTPEVEEIWRKGLEGDNENYASRTGYLDRIRSIVTQT
                     AQDPEKAIAGAEEPPHGTVNFIARDRDGHIACAVSTSGWAWKYPGRLGDSPIIGAGNY
                     ADDRWGAAGCTGRGEMAQRCLTAHSVVTFMRFGMSLEDALKTAMTDLDRLVDPYKSEM
                     NIVAMDKEGNPSAASSALGKTFVFMREDMSTFEEAERLHVPIEGVASSL"
     gene            complement(2252..2533)
                     /locus_tag="VFQ54_08625"
     CDS             complement(2252..2533)
                     /locus_tag="VFQ54_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEU0165096.1"
                     /translation="MSAELPPIIMYARTRFCPDVTRSRGRLTELELTWQEFDIEADDA
                     AAQKTEELTGHRSVPTIVIGDSILVEPSNAELDAALVKAGYAIEADAVR"
     gene            complement(2570..3607)
                     /locus_tag="VFQ54_08630"
     CDS             complement(2570..3607)
                     /locus_tag="VFQ54_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEU0165097.1"
                     /translation="MTDDPVTPNSIPLKAGKLPGPLLARLLATYATPDDDDVIVPSGY
                     GRDAAAIQLGDQQLIVKSDPITFATDGAARYLVAVNANDIACLGGIPRWLSVVALLPE
                     KTSSEESVEALFRDLQTACVAARISLIGGHTEITIGLDRPLLIGTMLGTAGAAGILQP
                     GGAKIGDDLYVSKWVGIEGTALLAREHADRLRPIVGPEIVRSAAKLLRSPGISVARDA
                     AILLATGRVHALHDPTEGGVATAIHEIAEASSLGAEIDVESVPMLPETRILCDHYGID
                     PLGLLSSGALLIVGDPDGRQDLERAATEGGVPLSWIGRIVSREDGVTMLGPGGRTAVP
                     RFDSDEVTRVL"
     gene            complement(3634..3900)
                     /gene="xseB"
                     /locus_tag="VFQ54_08635"
     CDS             complement(3634..3900)
                     /gene="xseB"
                     /locus_tag="VFQ54_08635"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU0165098.1"
                     /translation="MSTSTTPDQSMSLEEALAALQVIVEQLEQGNLPLAETIARFEEG
                     SRLAELCQKLIDDAELRITVLTEDDDELDADDRFDDEDPTDVPF"
     gene            complement(3974..>5175)
                     /gene="xseA"
                     /locus_tag="VFQ54_08640"
     CDS             complement(3974..>5175)
                     /gene="xseA"
                     /locus_tag="VFQ54_08640"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU0165099.1"
                     /translation="VERLTKYIRELLASDALLQDIWVEGEVSSFTVAASGHAYLTLQD
                     SNARIDAVMWKTAVRRQTFVPRVGDNIIVHGEVTVYERQGRLQLSADVFQPAGLGLLQ
                     LQLEVLRQKLEAEGLFDTGRKRPLPAFPLRIGVATSATGAVWHDIQTVIGRRFPLVEL
                     VLAPTLVQGTEAPDQIVAALSLLQSQTDIDLIILARGGGSAEDLWCFNDERVARAIFA
                     SRVPVVSAIGHETDVTIADYVADVRAATPSAAAELAVPAIEEIEGYVRLVRRRMTALV
                     QAEVAATHREIDGLTHRLGRMSPLRQLEQQRVRIAALKTRLSLATAHDLDRRKMDLSR
                     QRAVLSSLDPTAILKRGYGLITSEESGLPVRSVGTLVPGTRIRSTLINGTVTASVESI
                     EFNGRHG"
BASE COUNT          927 a         1595 c         1713 g          940 t
ORIGIN      
        1 accggcagcc gcatggacga cgtgatctac gaggagttca agggaaccgg taacatggaa
       61 ctccacctcg atcgcaagct ggctgagcgc cggatctacc cggctatcga catccagcgc
      121 tcgtcaacgc gacgcgagga gcttctgctc gacgacttca cgcttcgcca ggtctggacg
      181 atgcgccgca tggtctcgat gctcggcggc aacgagggca cggagctcat gctgagccgt
      241 ctctcgaaga cgcagaagaa ctccgagttc ctggagacgc tcaccaagga cgtctaggac
      301 ccaggacgga caagcgatcg ccaacaagtg aaggccctgg tcacacgacc agggccttca
      361 cttcgttgcg gtcagccaag ttcctcgtgg agcgtctgga aggtccaggt gggacaacca
      421 cagcccagaa agtcgcagtc gttcgtcaga atcccggcac ccaggtgaag agcgagcgcg
      481 atcggcgccc agtcgttggg atctcgcggg attcggcgcg cggcggtatc gcggagatga
      541 gaatagttga cggatggaat aacgcgcagg gcttcatcgt tgaacagtgt catgacttcg
      601 agtcgaagcc gctgctcctc gctggcggtc agtgtcctgt aatggacaag tcgttagatt
      661 cgtcggtcga gttcatggat cgcctcgctg tattgctcct ccgcgacaag cacgtcgagt
      721 tcgtcacggg agagaagtag ccgccctcgc cccgcaagag ctcggacacg agcacactcg
      781 catcaaccgc gacgatcatc ggccgtcgtc ttcgtattca ggagggagtg gtttgttcaa
      841 atcgaagagg tcagcgaatt cgtcctcggt catgccagtg cgttcgagaa tcgcctctac
      901 cgtgcgacca agatcgtcta gcgcgcgctt catatcttcc ttgtcgcgct caacgggaac
      961 gtagaatccc acgatcttgc cgtggcgact gatagcaacc ggtttgctgc tcgcaaaata
     1021 cctggtcgca tgatcccgaa attcgcgaat gccgacgttc tcgacatcgc tcgccgccag
     1081 atattgcgcg gctgctcttc ggaattcggc gagactcgct gctgcttcga ctctgtcctt
     1141 gatcgccatt gtggtcacct cctgaccaca atctagacgg cgatgtcccg cccgtcaagc
     1201 tgcagaatgc gagaacccca tcccgcaaag gatggggttc tcgcacccgc aatgatagag
     1261 acgtaccggg cgtggcatca gagcgaagag gcgacgccct cgatcggaac gtgaaggcgc
     1321 tcggcctcct cgaacgtgga catatcctca cgcatgaaga caaaggtctt cccgagggcg
     1381 ctggaggcgg cgctcgggtt cccctccttg tccatcgcga cgatgttcat ttcgctcttg
     1441 tagggatcga ccaggcgatc gaggtcggtc atcgcggtct tcagggcatc ttccagcgac
     1501 atgccgaagc gcatgaaggt gacgacgctg tgcgccgtca ggcagcgctg cgccatctcg
     1561 ccgcgaccgg tgcagccggc ggcaccccaa cggtcgtcgg cgtagttgcc ggcgccgatg
     1621 atcggggaat cgccgagtcg tccgggatat ttccaggccc agccggaggt gctgaccgca
     1681 caggcgatgt ggccgtcgcg gtcgcgggca atgaagttca ccgtcccatg gggcggctcc
     1741 tcggcgccgg cgatcgcctt ctccggatcc tgggcggttt gcgtgacgat cgaccggatg
     1801 cgatcgaggt agcccgtgcg actcgcatag ttctcgttat cgccttcgag gcccttgcgc
     1861 cagatctctt ccacctcggg ggtgaggaga ttcgacgagg tcaggcccat ctcggcggcg
     1921 aagcgagcac taccctgtcc cgcgatcagg acgtggggaa gctcctccat gacgtgacgc
     1981 gcgaggtcga cggcatcctg gtacccctgg agcgcgccga ccgagccggc gcgcaggccg
     2041 gttccttcca tcacgcttgc atcgagttcg acttcgccga ggagattcgg aagaccagag
     2101 aagccgaccg agtgatcctc gggattcgcc tcgacgagac gcgtaccggc gatgacggcg
     2161 tcaagcgccg agccaccggc cttcaggacc tcgatcgcag cctcgatgcc gacgcgcccg
     2221 ttggcggacg cggtgacgac gccgacgttg ctcatcgaac ggcgtccgct tcgatcgcgt
     2281 agcctgcctt gaccagtgcg gcgtcgagtt cggcgttgga cggctcgacg agaatcgagt
     2341 caccgatgac gatcgttggc acgctgcggt gacctgtcag ttcctcggtc ttctgggcgg
     2401 ccgcatcgtc ggcctcgatg tcgaattcct gccacgtcag ttcgagttcc gtcaggcgtc
     2461 cacgcgagcg ggtcacgtcg gggcagaagc gggtgcgggc atacatgatg attgggggaa
     2521 gttctgcaga catgaggcga ctcgctttcc ttgagaaatc ctgcacgcgt cagagcacac
     2581 gtgtaacttc gtccgaatcg aaccggggga ccgccgtgcg cccgccggga ccgagcatcg
     2641 tcacgccgtc ctcacgggag acgatgcgac cgatccagga aagcggcact ccgccctcgg
     2701 tcgctgcgcg ctccagatcc tggcgtccgt ccggatcgcc aacaatgagc agcgcgccgg
     2761 agctgagcag ccccaacgga tcgattccat aatgatcgca aagaatgcgc gtctctggaa
     2821 gcatgggaac cgattcgacg tcgatctcgg ctccgaggga cgatgcctcg gcgatctcgt
     2881 ggatcgcggt ggcgacaccg ccctcggtcg gatcgtgcag cgcatgcaca cgaccggtcg
     2941 cgagcaggat ggccgcgtcc ctggcgaccg agatgccggg gctgcggagg agcttggccg
     3001 cgctgcggac gatctccggc ccgacgatcg ggcggaggcg atcggcatgc tcgcgggcga
     3061 ggagcgcggt cccttcgatc ccgacccatt tgctgacgta gagatcgtcg ccgatcttcg
     3121 cgccgccggg ttggaggata ccggccgcgc cggcggtgcc gagcatcgtg ccgatcagca
     3181 gcgggcgatc gagcccgatc gtgatctccg tgtggccgcc gatcaatgag atccgcgccg
     3241 cgacacatgc cgtctgaaga tcccggaaga gcgcctccac tgactcctcg gaggatgtct
     3301 tctcgggtag cagggcaacg accgagagcc agcgggggat gccgccaagg catgcgatgt
     3361 cgttcgcatt cacggcgacg aggtaccgcg ccgcgccgtc ggtcgcgaag gtgatcggat
     3421 cgctcttgac gatcagctgc tggtcgccga gctggattgc ggccgcgtcg cgaccgtaac
     3481 cggacgggac gatgacgtcg tcgtcgtcgg gcgtggcgta ggtcgccagc aggcgcgcca
     3541 gcagcggccc gggcaacttg cccgctttca gggggattga gttcggagtc actggatcat
     3601 cggtcatgca gagggttgtc cttgggccga tggtcagaag ggcacatcgg ttggatcttc
     3661 gtcgtcgaat cggtcgtcgg cgtcgagctc atcgtcatct tccgtgagga cggtgatccg
     3721 caactcggcg tcgtcgatca gcttctggca gagctcggcg aggcgcgaac cttcctcgaa
     3781 ccgcgcgatc gtctccgcga gcggaaggtt gccctgttcg agttgctcga cgatgacctg
     3841 gagagcggcg agcgcctcct ccagtgacat cgattgatcg ggagtggtcg aagtgctcac
     3901 agctggcctt ccgtgcgagg tgcgtcgttc gtcgctgcaa ttgtatctgg caggatggat
     3961 tggaacagga gaatcagccg tggcgcccgt tgaattcgat cgactccacc gatgcggtga
     4021 cggttccatt gataagggtg gatcgaatgc gtgtccccgg gaccagcgtt ccgaccgatc
     4081 tgacgggaag cccgctttct tcgctggtga tgagcccata gccgcgtttg aggatcgccg
     4141 tcgggtcgag cgaggagagc acggcgcgct ggcggctgag gtccatcttg cggcgatcga
     4201 gatcgtgagc cgtggcgagt gaaagtcgcg tcttgagcgc ggcgatcctg acacgctgtt
     4261 gctccagctg gcgaagcggc gacattcgtc ccagccggtg cgtgaggccg tcgatctcgc
     4321 gatgggtggc cgcgacctct gcctggacga gtgcggtcat ccgacgacga acaagccgga
     4381 cgtagccctc gatttcctcg atggcgggta ccgcgagttc cgccgcggcc gacggtgtcg
     4441 ccgcccggac gtcggcgaca tagtccgcga tcgtcacatc ggtctcatga ccgatcgcgg
     4501 aaacgaccgg gacgcgtgag gcgaagatcg cccgggcgac gcgttcgtcg ttgaagcacc
     4561 agagatcttc cgccgaacct ccgccgcggg caaggatgat cagatcgatg tccgtttgcg
     4621 actggagcag ggagagagcc gcgacgatct ggtcgggagc ttcggtcccc tggacaagcg
     4681 tcggcgcgag cacgagttcc acgagtggga accggcggcc gatgaccgtc tgaatgtcgt
     4741 gccagacagc gccggtggcc gaggtggcga ctccgatccg aagggggaag gcgggaagcg
     4801 gtcgcttgcg tcccgtgtcg aagagtccct ccgcctcgag cttctggcgg agcacctcca
     4861 gctgcagttg cagcaacccg agcccggcgg gttggaagac gtccgcggag agctggaggc
     4921 gtccctgccg ctcgtagacc gtcacctcgc cgtggacgat gatgttgtcc ccgacacgcg
     4981 gcacgaaggt ctggcggcga acggccgtct tccacatcac ggcgtcgatc cgggcgttgc
     5041 tgtcctgcag cgtgaggtag gcgtggccag atgcggcgac cgtgaagctg gagacctcgc
     5101 cctcgaccca gatgtcctgc agaagtgcgt cagacgcaag aagctcacga atgtatttcg
     5161 tgagcctctc gacat
//
LOCUS       DASTMD010000588         1810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_679395, whole genome shotgun sequence.
ACCESSION   DASTMD010000588 DASTMD010000000
VERSION     DASTMD010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679395"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFQ54_08645"
     CDS             <1..544
                     /locus_tag="VFQ54_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEU0165100.1"
                     /translation="PPITWVGSPNFFANRAGVGNPTDIVYHCTDDLVLANTTGWFLNP
                     SSKASANFVIDRDGTVYQFVSSKDGPWSNGEINKPRTDIPELTRILTSGMNPNDYCIT
                     YEFVATPETPPTQAQYDAAIRLSRYFCHPGVYAINPNRSHQLRHADIDSVNRANCPGP
                     HFDLTQIITAIGGDPNQIAA"
     gene            613..1008
                     /locus_tag="VFQ54_08650"
     CDS             613..1008
                     /locus_tag="VFQ54_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165101.1"
                     /translation="MNTVQKEPLLTAGTAVGILGAALIVLQKFHVPISDDQVTALKNF
                     FVVAGPVLLALIARQWVWSSPNHDAAVADAIETTGVVKASTTTPAIAAPGLVATSPVT
                     RVEAPPKEAPVPVAVRQTASPVMTDVTAS"
BASE COUNT          354 a          575 c          560 g          321 t
ORIGIN      
        1 gccgccgatc acctgggtcg gcagcccgaa cttcttcgcc aatagggccg gcgtcggcaa
       61 tccgaccgac atcgtctatc actgcaccga tgacctcgtg ctggcgaaca cgaccggctg
      121 gttccttaat ccttcgagca aggcgtcggc gaacttcgtg atcgatcgcg acggcacggt
      181 ctaccagttc gtctcgagca aggacggacc gtggtcgaac ggggagatca acaagccgcg
      241 gaccgacatc cccgagctca cccggatcct cacgtcgggc atgaacccga acgactactg
      301 catcacctat gagttcgtgg ccaccccgga gacgccgccg acccaggcgc agtacgacgc
      361 ggcgattcgc ctcagccgct acttctgcca cccgggcgtc tacgcgatca acccgaaccg
      421 ctcgcatcag ctgcggcacg cggacatcga ctcggtcaat cgcgcgaact gcccgggtcc
      481 gcacttcgac ctgacgcaga tcatcaccgc gatcggtggc gatcccaacc agatcgcggc
      541 ataggaacgg aaacgtaagg aacgggaatc gctccttacg aaaacgcccg atttcgttca
      601 caaggagctc tcatgaacac cgtgcagaaa gaaccgttgc tcaccgcagg aaccgccgtg
      661 gggatcctcg gcgcggccct catcgtcctt cagaagttcc atgtcccgat cagcgatgac
      721 caggtgaccg cgctcaagaa cttcttcgtg gtcgccggtc cggtgctgtt ggcgctgatc
      781 gcccgtcagt gggtatggtc ctcgccgaat cacgacgccg cggttgctga tgcgatcgag
      841 acgacgggtg tggtcaaggc ctcgaccacg acgccggcga tcgccgcgcc ggggctggtg
      901 gcgacgtccc cggtgactcg cgtcgaggcg ccgccgaagg aagcgccggt gccagttgcg
      961 gtaaggcaga ctgcctcccc agtaatgacg gacgttacag catcgtagcg tcttttcagg
     1021 tcgtgggcga attgctaaaa tcggcgggcc atcccctccg ccggaggaat atggacgcct
     1081 ttgggcgcgt tagggctgcg aacctgatgc gctcattggt tgtcggcatg tccggttcga
     1141 agagaacacc atcgagggac cgatacgagt gctccggcag atccagtccg tgccaacggt
     1201 cgcacgggaa atatccgcac acggcgacgg cagaggcggc gcatgctcgc cgcctctgcc
     1261 ttggcgccat agagaccatg gtgagcattc cgctcaccat ggtctctatg aatggaccga
     1321 tccgttcttt ggcaccaccg cgggagacga tgccatgatc accccggagc aatgcgagcg
     1381 gtactggatg cgttatgcat cggcggttcg ctcgttgccc gaatccgcat ccgtggcgac
     1441 ccgtcaccta gcggggctcg agtccgttgt cctccaagca cagcacgaga tcgtgcatcg
     1501 tctgattcgc gatgcacgac aagaggtcga acaatccgac tatgcgggta cgtgcgagcg
     1561 cgcgaaagca ctccacttgc atctgaatcg actcattcgc ctcgccgtgg atctggaggc
     1621 ggagggtcca atgactgacg accgcgatgg caggtacgcc ccttagccct ccgacgatcc
     1681 ggactggctc gactggctgg cggatgcgat cgccgaggag tgggaggacg catgagcgag
     1741 gatgatcgta agaagcgggt aaaccatctc gggttcgggg accggcagtg gatcggtcgg
     1801 ttcttccgtg
//
LOCUS       DASTMD010000589         1683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2288078, whole genome shotgun sequence.
ACCESSION   DASTMD010000589 DASTMD010000000
VERSION     DASTMD010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2288078"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="VFQ54_08655"
     CDS             complement(<1..1605)
                     /locus_tag="VFQ54_08655"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEU0165102.1"
                     /translation="MNFHPRKAKWMLGLLLLLVSLFAAPSGADAHALLSTSAPANGDI
                     IATEPSTVTATFTEHVEPSESTMELFDSTGRQVTGPKLSVSSDHYSMSLTMPANLPNG
                     TYSVLWRTLSADDGHTAEGYFSFTIGSAADVSSVVAVPTDNVSSGPPQLVKTASRWAA
                     LVGFAAFIAAWPIWTIVIRPSLAPIWQEGPRFTRRARRYVLIAFVVAILGSLFALAIQ
                     ALAIPDGSYFDKVMNTVGKTRYGRLWLFRVGLMMVEGLILSACAWWYVRHRKMEWAAA
                     WLVAIAMPIPFSLIAHASAQPIGRDLSVVADVVHLLSAGIWVGGIFMLTFVLFPGLRR
                     IDPVKRREVLIVAIPRFSLISMIAWSVLGLTGFYAGWVQVGNLHALGTTSYGHSLIVK
                     LILLAIVLIVASVNLFVIERHLTRKIDERAINIWSRRLTWTVATELVLILGVLGAVGQ
                     MTSQEPARDVLVERSNQITVSFKDMDPQAKLILAPGIAGVNHFRLEVGGPALPTDTVA
                     LIYLTMPGNDKLGTGQVTLARVAGNAF"
BASE COUNT          312 a          503 c          598 g          270 t
ORIGIN      
        1 gaaggcgttt cccgccacgc gtgccagtgt cacctgtccc gttccgagct tgtcattgcc
       61 cggcatcgtc aggtagatca gggcgacagt gtcggttggc agcgcggggc cgccgacctc
      121 gagccggaag tgattgacgc cggcgatgcc gggcgccagg atcagcttcg cctgcgggtc
      181 catgtccttg aaggagaccg tgatctggtt ggagcgttcg acgaggacat cgcgcgccgg
      241 ttcctggctc gtcatctggc caacggcgcc gaggacgccg aggatcagca cgagttcggt
      301 cgctaccgtc caggtcagtc gccggctcca gatgttgatc gcccgctcgt cgatcttccg
      361 cgtcaggtga cgctcgatca cgaagaggtt gaccgacgcc acgatcagga cgatcgcgag
      421 gaggatcagc ttcacgatca gggaatgacc gtaggaggtc gtcccgaggg cgtgaaggtt
      481 tccgacctgc acccaacccg cgtagaagcc ggtcagtccg aggacggacc aggcgatcat
      541 cgagatcagc gagaaccggg gaatcgcgac gatcagcact tcccggcgct tgacgggatc
      601 gatcctgcgg agaccgggga agagcacgaa cgtgagcatg aagatgccgc cgacccagat
      661 cccggcggag agcaggtgca ccacatcggc gacgaccgag aggtcgcggc cgatcggttg
      721 cgccgatgcg tgggcgatca ggctgaacgg gatcggcatt gcgatcgcga cgagccaggc
      781 ggccgcccac tccatcttgc ggtgccgcac gtaccaccaa gcgcaggcgc tgaggatcag
      841 tccctccacc atcatcagcc ccacgcggaa gagccagagg cggccgtacc gggtcttgcc
      901 gacggtgttc atcaccttgt cgaagtagct gccatccggg atcgccagcg cctggatcgc
      961 cagcgcgaag agcgagccga ggatcgcgac gacaaacgcg atcagcacgt agcggcgggc
     1021 gcggcgggtg aagcgcggtc cctcctgcca gatcggggcg agcgacggtc ggatcacgat
     1081 cgtccagatc ggccaggctg cgatgaaggc cgcgaagcca acgagtgccg cccagcgcga
     1141 cgcggtcttt accagctgcg gggggccgct gctgacattg tcggtcggga cggcgacaac
     1201 ggaagagaca tcggcggcgg agccgatggt gaagctgaag tagccctcgg cggtatggcc
     1261 gtcgtccgcc gacagggttc gccagaggac ggaatatgtc ccgttcggga ggttggcggg
     1321 catcgtcagc gacatcgagt agtggtcgga actgacgctg agcttcggac cggtaacctg
     1381 tcgacccgtg ctgtcgaaga gttccatcgt gctttccgat ggttcgacat gctcggtgaa
     1441 tgtcgcggtc acggtcgagg gctcggtggc gatgatgtcg ccattagccg gcgcactcgt
     1501 gctgagcagg gcgtgggcat cggcgccgga cggcgccgcg aagagcgaga cgagcagcag
     1561 gagcagaccg agcatccact tcgcttttcg agggtggaag ttcatgttcg ctccgcgtgg
     1621 atgggggaaa gggaacctgt gcatgacggg ccttcgtcct gggtgcggca gggagtcgcg
     1681 act
//
LOCUS       DASTMD010000590         6080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2733660, whole genome shotgun sequence.
ACCESSION   DASTMD010000590 DASTMD010000000
VERSION     DASTMD010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2733660"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(324..1595)
                     /locus_tag="VFQ54_08660"
     CDS             complement(324..1595)
                     /locus_tag="VFQ54_08660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165103.1"
                     /translation="MVDAVTKATTSIRRGALIFYTQINLFGLGLLGFWTVINVLLLPT
                     MAQDMAPASLTGSAVGLISVVGIGLAMIVQLVAGRASDAWRHDDARRPFIVAGLVLIV
                     PGSAIFGFASSFPLLLLGFVLLQVATNISQAAFQALIPDLVDEDERGIASGVKNGLSV
                     LGAVVGLLGGEGLYALTNSAPVAVAYVALLLVVTGALTAYWAPKSSSPEGGDPGSIRE
                     AFSIPSMLDEFRQVLDTRVTFRLAVIAQFLFFLGTYPAQRFLLLFLEDRFGDDARIWV
                     AVGGAAAILVAVIAAIAGGALSDVIGRRNVLIGSAVIGSSGLVLIGFAPTILIAGIAG
                     SLIAVGLGAFQSVNWALMNDDLPEGQPAGALGIANVATTGAGAVAGLFGPFADLVNAV
                     APAATYQALFAVAGLIAVLSVLPLRRVVESE"
     gene            2066..3475
                     /locus_tag="VFQ54_08665"
     CDS             2066..3475
                     /locus_tag="VFQ54_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HEU0165104.1"
                     /translation="MLLDIHPGLPPAARRRQNALRLGRATILAMIALGTVVAATAIWV
                     RSQGIPAFRTYDGGLGSLGLLTGLLASDLVIIQVLLIARIPWVETAWGHDVLLRYHRW
                     VGFASFWFMIAHVYTYTVLRVEGARGALSDRLWQLFVTNPWMLFATIGTIMLIAVVVS
                     SIRNARRRLRYESWHFLHLYAYLGMAFAFPHQLFDGRIFHGTWSRFFWWGIYILAAAA
                     IVIFRLGLPLWRSWYHQLRVSNVRSEAPGIVSIAMSGQRLDRLDVRSGQFFIWRFLSG
                     GGLGWATGHPYTLSAAPTDDHLRITIQDAGDWSHRVARLREGTRVLIEGPYGVMTADR
                     RRHRRLLCIAAGVGITPMRALLADADYAPGDATLIYRVGSPDQTIFHDELDAIATARG
                     VDVIYVSGPRASQSSWLPAGPDGWDQDGVAVLRELIPDVADRDVFICGPSGWMSLVRK
                     SLRQAGVARRDIHTENFSW"
     gene            complement(3509..4534)
                     /gene="gap"
                     /locus_tag="VFQ54_08670"
     CDS             complement(3509..4534)
                     /gene="gap"
                     /locus_tag="VFQ54_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU0165105.1"
                     /translation="MVYRVAINGFGRIGRQVFKAIEQGGFGELFEVVAINEFKPDTAH
                     LLRYDSTYGRFDAEIAENDAGLLVNGRQVHGVRERDPAKLPWAELGIDLVIESTGHFT
                     DAEQAKGHIEAGAKKVIITAPATGEDLTVVLGVNQHSYDPDRHHIISNASCTTNCLAP
                     VAKVVLDNFGIREGLMTTVHAYTSDQRLLDDPHHDLRRARAAGQNIVPTTTGAAQAVA
                     LVIPELQGKFDGISLRVPTPTVSLVDFVAITERLTTIEEINCVLKSAAESDALAGILG
                     YSEEPLVSTDYRQDSRSSIVDALSTQVVRENMVKIIAWYDNEWGYSCRVADLAAFVCE
                     NGIGAAS"
     gene            complement(4785..5030)
                     /locus_tag="VFQ54_08675"
     CDS             complement(4785..5030)
                     /locus_tag="VFQ54_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165106.1"
                     /translation="MTDRERVDELDDLAPRVEPAYATHDDLEALREQVKDLTAVVIRL
                     AHATKELGDVMRTSRDPATTPAAAFTAIDELVSEIDA"
     gene            complement(5082..5342)
                     /locus_tag="VFQ54_08680"
     CDS             complement(5082..5342)
                     /locus_tag="VFQ54_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165107.1"
                     /translation="MDENLPDDTIDQEDLADQLNQVRNDLAQLEEETARAEDLDTLRL
                     LVISLIAAVRDISEVVRGLPGIPADAAAKFDGISELSSEIGA"
     gene            5571..>6080
                     /locus_tag="VFQ54_08685"
     CDS             5571..>6080
                     /locus_tag="VFQ54_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0165108.1"
                     /translation="MTTHPATTVHALIAQHYDLPAPLAVKFLRAYTNDVYEVTTARDR
                     YVLKMYGAAWRTRSELRYEVELLHHLDASGVPVALPVVARDGDPVQTHHIGDGERHMM
                     LFAWAPGDKPAPPFLPELYAAEGRAIAAFHAASDDFVSDHARPALDTRYLVDEPMAVI
                     LGHLADPASA"
BASE COUNT         1062 a         2051 c         1896 g         1071 t
ORIGIN      
        1 ccgccctttc ctcgtaaaga cattcgcctc gcgcgatgga tattcccgtc gtagcggatc
       61 agctggtctg accgatcggg cgcagcccga tatgatcgag tcattcgcgc catatcatcg
      121 atcacgaaca tacggtgaat cgtagacgcg acccttgtgg tcgcaccgga ccagtttcgc
      181 cgggaggacg tgcccgggga ggcatcgtga cggcgcaatc tcatcctcgt ccccacggcg
      241 cttgtcgccg tttgccgggc gatcatgagg ctcgccccta cgaaccgagg gatttctcga
      301 caggctcgaa tggtcgatcg ccgtcactcc gattcgacga cgcgccgcag gggaaggacg
      361 ctgaggacgg cgatcaagcc tgccacggcg aagagcgcct gataggtcgc cgccggcgcg
      421 accgcattca cgagatcggc gaagggaccg aagagtcccg ccacggcccc ggcgccggtg
      481 gtcgcgacat tcgcgatgcc aagcgcaccc gcgggctgac cctcgggaag gtcatcgttc
      541 atcagcgccc agttcacgga ctggaatgcc ccgagaccga cggcgatcag gctgccggcg
      601 atcccggcaa tcaggatcgt cggcgcgaac ccgatcaaca cgagcccgct gctgccgatc
      661 accgcactcc cgatcaggac gttccgccgc ccgatgacat cggagagcgc acccccggcg
      721 attgccgcga tcaccgcgac gaggatcgcc gccgccccac ccacggcaac ccagatcctc
      781 gcatcatcgc caaaacgatc ctcgaggaag aggagcagga agcgctgggc gggatacgtc
      841 ccaaggaaaa agagaaactg cgcgatcacg gcgagacgaa aggtgacgcg ggtgtcgagg
      901 acctgccgga actcgtcaag catcgacggg atgctgaacg cctccctgat cgacccggga
      961 tcgccacctt ccggcgagga gctcttcggc gcccagtacg cggtaagcgc gccggtcacg
     1021 accagcagca gcgcgacata ggcgaccgcg accggcgcgg aattcgtgag cgcatagagt
     1081 ccctcgccac cgaggagtcc gaccacggcc ccgagcacgc tcagcccgtt cttgaccccg
     1141 gacgcgattc ctcgctcatc ctcgtcgacg agatccggga tcaacgcctg aaaggccgcc
     1201 tgggagatat tcgtcgcaac ctgcagcagc acgaatccaa gcaggagaag cgggaagctc
     1261 gaggcgaagc cgaagatcgc tgatcccgga acgatcagga cgagaccggc aacgatgaac
     1321 ggccgccgcg catcgtcgtg tcgccaggca tcgctggccc tgccggcgac aagctggacg
     1381 atcatcgcga gaccgatccc gacgacactg atcaacccga cggcgcttcc cgtcaacgac
     1441 gcgggcgcca tgtcctgggc catcgtcggc aggagcagga cattgatgac ggtccagaag
     1501 ccaagcagcc cgagcccgaa gaggttgatc tgcgtataga aaatgagcgc cccacgacgt
     1561 atcgaagtgg tggctttcgt taccgcatca accacgaacg gcctctctcg catcatccat
     1621 gagcacacgc ccgtcgcgct cctgggcagc atgacgccgg aacgatgcca tcgcgccacg
     1681 cgaatcgttc ccccatcatg cgtccgccgc ccccacccgg ccagccccgg cgatctccga
     1741 gcggatcggc gctgccgcat tgatccgagg cgaccacgtg ggtcgccggg acgggaaccg
     1801 gacggacgca gggagcaccg cctcaaaacg cgcctggcga ggagcacgct ccttgtcgtc
     1861 cccacggcgc ttgtcgccgt tccggctgaa acagaaacgg cttcaggggt ccgctcaagg
     1921 cggcaatctg ccccatcgcg acgatccggg acgtacccta tgaagacgac gggaggcaaa
     1981 ccggtgcctg gtccggacca ggatcattcc tgcacaggct tcgaggctcg gcgatctcac
     2041 cgccaggtca agagggatcg acgatatgct tctggacatc catcctggcc taccgccagc
     2101 cgcccggcgc cggcagaatg cgcttcgcct cggccgggcg acgatcctcg cgatgattgc
     2161 gctcggaacc gtggtcgcgg cgaccgcgat ctgggtccgc agccagggca tcccggcatt
     2221 ccggacctac gatggcggtc tcgggtcgct gggcttgctc accggcctcc tcgcctccga
     2281 cctcgtgatc atccaggtcc tgctgatcgc acgcattccc tgggtcgaga cagcctgggg
     2341 ccacgacgtt ctcctgcggt atcaccggtg ggtgggcttc gcctccttct ggttcatgat
     2401 cgcgcacgtc tatacctaca cggtgttgcg cgtcgaagga gcccgcggcg cgctgagcga
     2461 ccggctctgg cagctgttcg tcacgaatcc gtggatgctc ttcgcgacga tcggcacgat
     2521 catgctgatt gcggtcgtcg tctcctcgat ccggaacgct cgacggcgac tccgctacga
     2581 gtcatggcat ttcctccatc tctacgccta tctgggaatg gccttcgcct tcccgcacca
     2641 gctcttcgat ggcaggatct tccacggcac gtggtcgcgg ttcttctggt gggggatcta
     2701 tatcctcgcc gcggcggcga tcgtcatctt ccgcctcggg ctcccgctct ggcgttcctg
     2761 gtatcaccag ctacgcgtct ccaacgtgcg aagcgaggcg ccgggcatcg tctcgatcgc
     2821 gatgagcggc cagcgactcg atcggctcga cgtgcgctcc gggcagttct tcatctggcg
     2881 cttcctcagc ggtggaggcc tgggctgggc cacgggtcat ccatacacgc tctccgccgc
     2941 cccgaccgac gatcacctcc ggatcacgat ccaggatgcc ggcgactgga gccaccgcgt
     3001 ggcgcgactg cgcgaaggga cccgggtcct gatcgagggt ccctacggcg tgatgaccgc
     3061 ggaccgccgc cggcatcgcc gcctgctctg catcgcggcc ggtgtcggga tcacaccgat
     3121 gcgcgcactc ctcgcggatg ccgattatgc ccccggcgat gcgacgctga tctaccgcgt
     3181 cggcagtccg gaccagacca tcttccatga tgagctcgac gcgatcgcca ccgcccgtgg
     3241 cgtcgacgtg atctacgtgt caggaccgcg cgcctcgcaa tcatcctggc tgccggcggg
     3301 accggatggc tgggatcagg atggcgtcgc cgtgctgagg gagctgatcc ccgacgtcgc
     3361 cgaccgtgac gtcttcatct gtgggccatc cggctggatg tcgctggtca ggaagagcct
     3421 gcgccaggcg ggcgtggccc ggcgcgacat ccacaccgag aatttctcct ggtaacaggg
     3481 cttcgggcag agatgggcaa gacccgcgtc agctcgccgc gccgatgccg ttctcgcaga
     3541 cgaacgcggc cagatcggcg acgcgacacg agtatcccca ctcgttgtcg taccacgcga
     3601 tgatcttgac catgttctcg cggacaacct gggtcgacag tgcgtcgacg atcgaggaac
     3661 gcgaatcctg ccggtagtcg gtggagacga gtggctcctc ggaatagccg aggatgcccg
     3721 ccagggcgtc actctccgcc gccgacttca ggacgcaatt gatctcctcg atcgtggtca
     3781 gccgctcggt gatggcgacg aagtccacga gcgagaccgt cggtgtcggg acccgcagtg
     3841 agatgccgtc gaacttcccc tggagttccg ggatgacgag cgccacggcc tgcgcggcgc
     3901 cggtggtggt cggaacgata ttctggcccg cggcgcgggc ccggcggaga tcgtgatggg
     3961 ggtcatccag caacctctga tcggaggtgt aggcgtggac cgtcgtcatc agcccttccc
     4021 tgatcccgaa attgtcgagc acgaccttgg cgacgggcgc gaggcagttc gtcgtgcagg
     4081 aggcgttgga gatgatgtga tgccggtccg gatcgtagga atgctgattg accccaagga
     4141 cgacggtcag gtcctcgccc gtggccgggg cggtgatgat gaccttcttc gccccggcct
     4201 cgatgtggcc cttcgcctgc tccgcatcgg tgaaatggcc ggtcgattcg atcacgaggt
     4261 cgatcccgag ttccgcccag ggaagcttcg cggggtcccg ttccctcaca ccgtgaacct
     4321 gacgaccgtt gacgagcaaa cccgcgtcat tctccgcgat ctcggcatcg aagcggccat
     4381 aggtggaatc gtacctgagg aggtgtgccg tgtccggttt gaattcgttg atcgcgacga
     4441 cttcgaagag ttccccgaaa ccaccctgtt cgattgcctt gaatacctgt cgcccgatac
     4501 gtccgaatcc attgatcgca acccgataca ccatgcgtct ctctctccgg gaccagggaa
     4561 ggtctgatcc atgctggctt tcaagcgcat cgatgcgaca ggcaagatcc gatcgcagca
     4621 cgttgctctg agcgcgcacg acagcgggca tctctcgtga ccggattgca ttctgtgcag
     4681 gtattgtacc ggaggaatgc gcaatctggg ccaacgtatg tgtcgttggt gctcgattct
     4741 gcggatcctg gcgttacgag cggaggtccc cgggcagtgt ctcgctatgc gtcgatctcg
     4801 ctgacgagct cgtcgatcgc ggtgaacgct gcggctggcg tcgtggcagg atcgcgggac
     4861 gtccgcatga cgtctcccag ctccttcgtc gcatgggcga gacgaatgac aacggcagtc
     4921 aggtccttca cctgttctcg caatgcctcg agatcgtcgt gcgtcgcata tgccggctcg
     4981 accctcggcg cgaggtcatc tagttcatca acccgttcgc gatcggtcat gtcagggctc
     5041 cttgctcggg caattcaatt caaggctggc gccgatcggc gctaggcacc tatctcgctg
     5101 gacagttcac tgatgccgtc gaacttcgcc gcggcatccg ccggtatccc cggaagcccg
     5161 cgcacaacct cgctgatgtc tctcacggcc gcgatcaggc tgatcaccag caggcgcagc
     5221 gtgtcgagat cctcggcgcg cgccgtctct tcctcgagtt gcgccaggtc gttgcggacc
     5281 tggttcaact ggtcggccag gtcctcctgg tcgatcgtgt cgtctggcag gttctcgtcc
     5341 atgctggttc cctttccgtg ttgtcccgct cgtcgcccca atgcgatcgg cttcggcgga
     5401 cgcgaggtcg aggtcgatgt cgttccaggg tcacgcaaca tccgtgcaat cgttgccgtg
     5461 gcgatagggg agggagcacc agggatcggt tcggacggca atcgcggcgg ccacaatcac
     5521 cggtatccac gagacccggg cggcgcgatc cgggctggta ttcttggccg atgacaacgc
     5581 accctgccac caccgtgcac gcactgatcg cccagcacta cgatctcccc gctcccctgg
     5641 cggtgaagtt ccttcgcgcc tataccaacg acgtctatga ggtcacgacg gcacgcgacc
     5701 gctacgtcct caagatgtat ggcgcggcgt ggcggacacg atcggagctt cgctacgaag
     5761 tcgaactcct gcatcacctc gacgcgagcg gagttcccgt ggcgctcccc gtcgtcgcca
     5821 gggatggcga tcccgtccag acccatcaca tcggggacgg ggaacggcac atgatgctct
     5881 tcgcctgggc acccggcgac aagccggcgc cgccattcct cccggagctc tacgccgccg
     5941 aaggacgcgc catcgccgca ttccacgccg cgagcgacga tttcgtctcg gatcacgccc
     6001 gaccggcgct ggatacccgc tatctcgtcg acgaaccgat ggccgtcatc ctcgggcacc
     6061 tggcagatcc cgcgagcgcg
//
LOCUS       DASTMD010000591         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2733705, whole genome shotgun sequence.
ACCESSION   DASTMD010000591 DASTMD010000000
VERSION     DASTMD010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2733705"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1053
                     /locus_tag="VFQ54_08690"
     CDS             169..1053
                     /locus_tag="VFQ54_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165109.1"
                     /translation="MSDFFTISLFTAVLRMCTPLLFTALGGLLSERSGVMNIGLEGMM
                     LTGAFFAVAGSYWTGNAWLGLIIGVLAAGGASAVHAFWCIQFQANQIVTGTAINLLAG
                     GLTAFLLQVIWGKAGGSDSVDKLPNIASGVNVLVPVAFVLVPLVHYFLFRTKPGLRVM
                     AAGEHPQAAESVGINVNRMRYAAVIVGGMLAGFGGAFLSIGDLSYFTLDMTTGRGFIA
                     LAAVIFGGWLPFGVLGATLLFGASQAIQIQAQATTGFPISSDLLVALPYLVTLVAITG
                     LVRSSNAPAGLGKHAVAH"
BASE COUNT          262 a          512 c          521 g          334 t
ORIGIN      
        1 cgtccggact gcggtccggt tgctgcggtc ccgcccgccg aacgaccgga tgcatcgagg
       61 aacatctcgt ccgatccgac cgtatgtgtg gcgattgtcc aggttttcgc tgtagtctcc
      121 aggcacgttc ccggtcgcat ccctgcgacc tgcagaaggg aagtcaggtt gtcagatttc
      181 ttcacgatat cgctcttcac ggccgttctc cggatgtgca cgccgctgct cttcacggcg
      241 ctcggcggcc tgctttcgga gcgatccggt gtgatgaata tcggtctcga agggatgatg
      301 ctgaccggcg ccttcttcgc cgtcgcggga tcgtactgga cgggcaacgc gtggctcggc
      361 ctgatcatcg gcgtgctcgc cgcgggtggg gcatccgccg tccacgcgtt ctggtgcatc
      421 cagttccagg cgaaccagat cgtcaccggg acggcgatca atctcctcgc cggaggattg
      481 acggcattcc tcctccaggt catctggggc aaggccggcg gcagcgactc cgtagacaaa
      541 ctgccgaaca tcgcctccgg cgtgaacgtc ctggttcccg tcgccttcgt gctggtcccg
      601 ctcgtccatt actttctctt ccgcacgaag cccggcctgc gggtgatggc ggccggcgag
      661 catccgcagg cggcggagag cgtgggcatc aatgtcaacc ggatgcgcta cgccgccgtg
      721 atcgtcggtg gcatgctggc tggattcggc ggcgccttcc tctcgatcgg ggatctttcc
      781 tacttcacgc tggacatgac caccggacgc ggcttcatcg ccctcgccgc cgtgatcttt
      841 ggcgggtggc ttccgttcgg ggtacttggc gcgacgctcc tcttcggcgc ctcgcaggcg
      901 atccagatcc aggcccaggc gacgaccggc ttcccgatct cctccgacct gctggtcgca
      961 cttccgtatc tggtgacgct ggttgccatc accggacttg tccgctcctc gaacgcaccg
     1021 gcgggtctgg gcaagcacgc ggtcgcgcac tgacgcgcgg gccgaccggg ctttgcacga
     1081 cgcgcgtgtg ttttcgggac cggtggacgc tgccgttgct gggaatcgac cttcaaatcg
     1141 aacatataca caagatttcg ttctcgccca tcccccgata ggactactgg agacgatcga
     1201 ggtaggaaga tcgcggcgac agtcgagacc gcctgtgaca gtacatccgt atgtcctatg
     1261 aacaaaccaa atcctgtaga ctacgtgaaa tgcgcggtac acccggagtt cttgagagcg
     1321 cgggagcggg acatgcagga gcgatcgact ccgcctggcg cagccgcgca tgcactgccc
     1381 gggagaggca gtgtcgcccg tgacttcatg ttgcgagtgg gtcccatcca gttgttcatc
     1441 acgtcaggaa cgtgaggggg agtattcggg catgagtgac acggttcgag tcgtcgtcgt
     1501 cgaacgtgaa ccaatctacc ggcgcggact ggccagcgtg ctggattccg gtgaggggat
     1561 cagcgtcgtc gggaccgccg gtactgcgga ggacgcgtac aagttgctcg atgagacgtc
     1621 cccggatgt
//
LOCUS       DASTMD010000592         9777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2733752, whole genome shotgun sequence.
ACCESSION   DASTMD010000592 DASTMD010000000
VERSION     DASTMD010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2733752"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..477
                     /locus_tag="VFQ54_08695"
     CDS             208..477
                     /locus_tag="VFQ54_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165110.1"
                     /translation="MTSGPTRPSNEGPPPWLSRYLSAMYDLRDAITVVRIHAQLAQRR
                     LARGIPGADSEAHSLAAIELASREAEWAIEALELEHLGSSGSDTD"
     gene            complement(500..1042)
                     /locus_tag="VFQ54_08700"
     CDS             complement(500..1042)
                     /locus_tag="VFQ54_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165111.1"
                     /translation="MADRVGYRMKSRHGTARNVSPIGACTELPGDARSKRRNSSGPFH
                     AGQASLLEPLPAPHIVVLEPDIEILGRIGHRLHREGLSVTMVSGALDVTTLRRIMPDV
                     IVLGLADAGAMETRALIESIRADDVLQRTSAICCAIGPDDDEYLTLRRVFPLPYPFDA
                     DDLVASVLGELSTRKANPEW"
     gene            1188..1724
                     /locus_tag="VFQ54_08705"
     CDS             1188..1724
                     /locus_tag="VFQ54_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0165112.1"
                     /translation="MNETRNESLGQVIRRRRAELGLTQEELAERVGPTVRQAEISRLE
                     NDRVTLPRRARLQTIADALELPLGMLLERSGWTGAGQIFNGDGEEHDHVPAPIERDAL
                     SPALPSWSHRGIEEHAHLRSELTRAISKSTMLLHETAAIMQQVHNSVLKLRQITVREI
                     PTIPPARDPGNSGLQRGG"
     gene            1746..2780
                     /locus_tag="VFQ54_08710"
     CDS             1746..2780
                     /locus_tag="VFQ54_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEU0165113.1"
                     /translation="MDDFARSVVVIGASAGGIQPLVELVSTFPRDFPAAVLVVVHIGT
                     GRSVLPRILSQAGSLPALHAVDNERIRAGHIYVAPPDYHLIVRDAHLGLSHGPKENHS
                     RPAIDPLFRSAAQSFNTHTIGIVLSGALSDGTSGLLAIKARGGQTLVQDPGEASVTGM
                     PESALRMQAADHVMRAREIGLALPSLLARAASDVVWTPTAPASGKGEPTAAASPAGTV
                     ISRDFSQQVDDRQHDRLTMFTCPDCGGSLWQIDGDGGLSFQCHVGHSWSPESLRGHKS
                     EQLEAALWASVRLLAERATLTRQLAKRMRREGNDLGANDIDDQAVIDEEHAATLRAVL
                     EKPQTTEDTT"
     gene            2921..5464
                     /locus_tag="VFQ54_08715"
     CDS             2921..5464
                     /locus_tag="VFQ54_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEU0165114.1"
                     /translation="MEASQAPSGSRYSHVVVIGASANGIESLQAIFSSLQGTFPAPIV
                     IAQHLDPDRASTRGEALDWIGTLPIQIVGELTPLEPGVVYVVPLGHSVEIQADILVPV
                     EGENYPQSSIDLLFTSAAASMEENVIAIVLAGTGSDGAIGAREVKYAGGTVIIENPEA
                     TAPSGIPVSLSPSVVDIVANRDRIGELLNELLAGAFVIPPVSEHAQLQQLLEDLREES
                     GIDFTSYKYATIERRLQRRMAATEQDTITDYIRHLHAHPEEYQRLVNGFLIKVTEFFR
                     DPDLFAYLGEHVLPELIREAKEKGSDLRIWSAGCATGEEAYSLAMLVADVLGDDWQNI
                     NVRIFATDLDEEAVAFARRGIYPGRSLAALSSSMVHRHFIERGGDYEVRKSLRTMLVF
                     GEHNLGQRAPFPRIDLILCRNVLIYFTPALQRRSLQLFAFSLRSGGYLVLGKSETVSP
                     LAEFFAVDQPRMKVFRRIGNRALIPPSRIRDAMPTALPILAARSKPFPGASRVARPGR
                     SRATWQSENLLLDLPVGVIVVDRHYDLQFINGVARRLLGIHAAAVEQDLVLLVQHFDA
                     VAVRRAIDAVFSGEANVNVLLSTVGEPTDAQQTIELTGRPIAQGDGEADELVALTIVD
                     VTEQERLRQRHANVENVTSRLILTNAELLNANQDLSATISRLRAENEEMMVAAEEIQA
                     ATEEVETLNEELQASNEELETLNEELQATVEELNTTNDDLQARTVELQTLAIEGEISR
                     QQLRAILDALEIATIVMDRHGSIVIESETYRRVFQRQSDQSRGMVDEEGTPLPIGSTP
                     VHRAMNGEAFIQRFGISSGEGTTWYVANSVNIDLTDSDRLTLLSLREAE"
     gene            complement(5768..6229)
                     /locus_tag="VFQ54_08720"
     CDS             complement(5768..6229)
                     /locus_tag="VFQ54_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU0165115.1"
                     /translation="MATTDPIQVTREGLIEGLQQDLAREYKAIVQYVIYSQKLDGAAY
                     MGIAGELEVHAHEELDHAIAVAKQLDYFGAYPTHVPEPVEVPEDNEGMLWADLRAEDT
                     TIENYRTRIREAEALGEYALAEILQEIIKQEQDHQIDLASALGVVPDPRGR"
     gene            complement(6291..7169)
                     /locus_tag="VFQ54_08725"
     CDS             complement(6291..7169)
                     /locus_tag="VFQ54_08725"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU0165116.1"
                     /translation="MVSIRTLADRLVDASPWLDKVAGVAHAASTPVFGEKGPPALKDA
                     LYGTWLGHPLHPAVTDLPIGFWTSSAVFDAVGMEDAADFALKAGTVSAFAAAVTGIAQ
                     WYDLQHQDQPRRLGTLHGVLNIGATWLYGTSWFLRSHQHRGAGIAFSLAGGSVATLAA
                     SLGGDLSYRLGTGVSRTAFEEPTGKWAPAIAVADLPEATPTRVVVEGDPVVLVRQGER
                     VLAASATCTHLGGPLDEGELKDGCLTCPWHGSVFALADGHVVNGPATSPLTTYATRVR
                     DGQIEVIAAAERAHAA"
     gene            7735..8925
                     /locus_tag="VFQ54_08730"
     CDS             7735..8925
                     /locus_tag="VFQ54_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0165117.1"
                     /translation="MSLVQSDLSRAVDILHDLIAIPSVNPAYDPPSEGESAVATYVEC
                     WARSHGLAATRGVVLPGRENVIVRLETRPNAPILLLEAHMDTVSADTMPDPFAATVRD
                     GHLYGRGACDTKGSLAAMMAAVESLVAMRDELPCTVILLAAVDEETSGTGVRAWVEGG
                     HYADAAIVGEPTRLRVIHAHNGVVRGAIGVSGRAAHTSVAHEGINAIDGMAEVVLALR
                     GLNADLASRPGGATANGSFTVSIIAGGTGINIVPEQCVIHYDRRTVPGDQAENVLAGI
                     DATLDAVRTQRPDLTIVRHDPSLIDDALDTSPAEAIVVVASEANDALGLDPAPTTVPY
                     GSDGSKLSVIGGIPTVVYGPGDIAQAHGADEYVELSELTAAAAFYREVAMRFRGHRQV
                     SIHE"
     gene            9001..9645
                     /gene="pcp"
                     /locus_tag="VFQ54_08735"
     CDS             9001..9645
                     /gene="pcp"
                     /locus_tag="VFQ54_08735"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: protein motif:HMM:NF009676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU0165118.1"
                     /translation="MTTILLTGFEPFGGDATNPSWEAVKRVADAWDGPEDLHIAELPV
                     IFHVAGDRLRDLMREYQPDLVIATGLAGGRPQVTPERIAINVDDARIPDNAGNDPIDA
                     AIAEDGPAAYFSTLPIKAAVAAIREQGIPAAVSNSAGTYLCNHIFYAIMHEQAQQYPG
                     ARGGFVHIPYSPEMVADRPQPSLALDLTAKAIATIIRTSLVTSGDLALAGGAIS"
BASE COUNT         1805 a         3301 c         2975 g         1696 t
ORIGIN      
        1 ttcacgagcg tggcccgggt ttaccccacc aaatcatcct tcttccgtat cgtagggaga
       61 tgggtctctt gccgtccaag gcgttgcggt cgtggtactc gaccacggtc gaaggagatg
      121 tggggaagac gcgcgccgga ccaccacggc atgagacatg catgactgat cgcgtgaagc
      181 gcaacgtctc acgcaaggta tatgcacatg acttcgggac ccactcgccc atccaacgag
      241 ggcccaccac cttggctctc tcgctacctt tccgccatgt atgacctccg cgacgccatc
      301 accgtggtcc gcattcacgc ccaactggct cagcggcgcc tcgcaagggg aatcccaggg
      361 gccgattcgg aggcccattc gctcgccgcg atcgaactcg cctcccgcga ggcggagtgg
      421 gcaatcgagg cactcgaact cgaacacctt gggtccagcg gaagcgatac cgactgattc
      481 cagcccgcgg tgacacgcat caccattcag gatttgcctt gcgcgtgctc agttccccga
      541 ggaccgatgc caccaggtca tcagcatcga agggataggg cagagggaaa acccggcgca
      601 gggtgagata ttcatcgtcg tccggcccga tggcacagca tattgccgag gtgcgttgga
      661 gaacatcgtc cgcacgaatg gactcgatca acgccctcgt ctccatcgcc cccgcatccg
      721 cgagtccgag gacgatgaca tccggcatga tccggcggag cgtcgtcacg tccagcgccc
      781 cgctgaccat ggtcaccgag agtccctccc gatgcagcct gtggccgatc cggcccagaa
      841 tctcgatatc tggctcgagc acaacgatat gcggtgcggg aagcggctcc agaagcgatg
      901 cctgaccggc atgaaacggt ccgctcgaat ttcgacgctt cgagcgcgca tctcccggca
      961 attcggtaca tgctccgatc ggggagacgt tccgtgcggt gccatgacga ctcttcatgc
     1021 ggtacccaac tctatctgcc atcacctgaa tcatcggcat cgtgatcgca atataatcat
     1081 tgagtactgc ccggacgttt gccagcgaga cctgacgata tgcagggaat ggatattgct
     1141 tagtcccgaa tcgatcatga tcggttgctg caggaggtgg gtacccgatg aacgaaacgc
     1201 gtaacgaatc gctcgggcag gtgatccggc gaagacgggc cgaactgggg ctgacgcagg
     1261 aggaactcgc ggagcgggtg ggaccgaccg tgaggcaggc ggagatatcg cggctcgaga
     1321 acgaccgggt gacattgcca cggcgcgccc ggctccagac gatcgcggat gcgctcgaac
     1381 ttcccctggg catgctgctc gaacgatcgg gctggaccgg ggccgggcag atcttcaatg
     1441 gcgacggcga ggagcacgat catgttcccg cgcccatcga aagggatgct ctttccccgg
     1501 ctctcccgtc atggagtcac cgcggtatcg aggagcatgc ccatcttcgc agcgaactca
     1561 cccgggcaat ttcgaagtcc acgatgcttt tgcacgagac tgccgctatc atgcagcaag
     1621 tgcacaattc cgtcctgaag cttcgccaga tcacggtgcg ggagattccc acgatcccgc
     1681 ccgcccgcga tcccggcaac tcgggtctcc agcgcggagg ttgatgctcc ccggggagga
     1741 ttctcttgga tgacttcgca cggtcggtgg tcgtcatcgg cgcatcggca gggggcatcc
     1801 agcctctggt cgagttggtc tcgacattcc cgcgcgattt tccggccgcg gtccttgtcg
     1861 tggttcatat cggaaccggc aggagcgtcc tccccagaat cctctcccag gccggatccc
     1921 tccccgccct ccatgccgtt gacaatgaac gcatccgcgc cggtcacatc tatgtggccc
     1981 ctccggacta tcacctgatc gtgcgcgatg cccacctcgg cctctcccac ggaccgaagg
     2041 aaaaccacag ccgaccggcg atcgatcccc tcttccgatc cgcggcccag agcttcaaca
     2101 cgcacacgat cggcatcgtc ctctccggcg ccctctccga tggcacgtcc ggactcctcg
     2161 cgatcaaggc ccgtggaggc cagacactcg ttcaggatcc gggtgaggca agcgtcaccg
     2221 ggatgccgga aagcgccctg cggatgcaag cggccgacca tgtgatgcgc gccagggaga
     2281 tcggcctggc actgccatcc ctcctggcgc gcgcggcatc cgacgtggtg tggaccccga
     2341 cggccccggc atccgggaag ggcgaaccaa ccgccgccgc gagtccggcc ggaaccgtca
     2401 tctcccggga tttcagccag caggtcgacg atcgccagca cgatcgactc acgatgttca
     2461 cctgccccga ttgcggcggg agtctctggc aaatcgacgg cgacggcggt ctcagcttcc
     2521 agtgtcatgt cgggcattcc tggagtccgg aatccctgcg tggtcacaag tcggagcaac
     2581 tcgaagcggc actctgggcg tcggtgcgcc tgctcgcgga gcgtgccacg cttacccggc
     2641 agctcgcgaa acgaatgcgg cgcgagggaa acgacctcgg cgcgaacgat atcgacgatc
     2701 aggccgtgat cgacgaagag cacgccgcga cacttcgcgc cgtgctcgaa aagccgcaaa
     2761 cgaccgaaga cacaacgtga cggcgatcag cgccttccaa cccgtagcgg ataccgggtt
     2821 cgacaagatc acacaccagc attcatcttc gatatcctga accgggccac tctacgcatc
     2881 agcgggcacc tgattgccct gccccattcg aaagcgcgtc atggaagcat cacaggcacc
     2941 atccggttcc cgatactcgc atgttgtcgt gattggcgcg tccgccaacg gcatcgaaag
     3001 cctgcaggcc atcttctcct cgctgcaggg aaccttcccg gcaccgatcg tcattgccca
     3061 acacctcgat ccggatcggg cgtcgacccg gggcgaggcg ctggattgga tcggaactct
     3121 ccccatccag atcgtgggcg agctgacgcc gctcgagccc ggcgtggtct acgtcgtgcc
     3181 gttgggccat agcgtcgaga tccaggcgga catcctcgtt ccagtggagg gcgaaaacta
     3241 cccgcaatct tccatcgacc tgctcttcac atcggccgcc gcaagcatgg aggagaacgt
     3301 catcgccatc gtcctcgcgg gaaccggctc cgatggcgcc attggcgccc gcgaggtgaa
     3361 atacgccggc ggcacggtga tcatcgaaaa tccggaggcc accgcccctt ccgggattcc
     3421 ggtctcgctc tcaccctccg ttgtcgacat cgtcgccaac cgggatcgta tcggcgagct
     3481 cctgaacgag ctgcttgccg gtgcctttgt gattccgccc gtctccgagc acgcccagtt
     3541 gcagcagctc ctcgaggatc tgcgcgagga gagcgggatc gacttcacca gttacaagta
     3601 cgcgacgatc gagcgtcgtc tgcagcggcg gatggcggca accgaacagg acacgatcac
     3661 ggattacatc cgtcatcttc acgcccatcc cgaggaatac cagcggctcg tcaacggctt
     3721 tctgatcaag gtgacggaat tcttccgcga ccccgacctc ttcgcctatc taggcgagca
     3781 tgtcctcccc gagctgatcc gcgaggcaaa ggagaaaggc agcgatctcc ggatctggtc
     3841 tgcgggttgc gcgaccggag aagaagccta ttcgctcgcg atgctcgtcg ccgatgtgct
     3901 cggtgacgac tggcagaaca tcaacgtccg catcttcgcc accgatctcg atgaagaagc
     3961 ggttgccttc gcccgccgcg gtatctaccc cggacgatcg ctcgccgcgt tgtcgagctc
     4021 catggtccat cgccacttca tcgaacgcgg gggcgattac gaggtgagga agtccttacg
     4081 cacgatgctc gttttcggcg agcacaacct gggacagcgc gctccgtttc cccgcatcga
     4141 tctcattctg tgccgcaacg tgctgatcta cttcacaccg gcactccagc gtcgttccct
     4201 ccagctcttc gccttttcgc tgcggtccgg tggctatctc gtgctcggaa aatcggaaac
     4261 ggtcagtccg ctcgccgagt tcttcgccgt cgaccagccg cgcatgaagg tcttccgccg
     4321 gatcgggaat cgcgcgctga ttccaccatc ccgcattcgc gatgcgatgc cgacggcatt
     4381 gccgatcctg gcggcgcgct cgaagccgtt tcctggcgcc tctcgcgtcg cacgccccgg
     4441 tcgttcgcgt gcgacgtggc agagcgagaa tctcctgctg gatcttccgg tcggggtcat
     4501 cgtggtcgat cgccactacg acctccagtt catcaatggc gtcgcacggc gtctgctcgg
     4561 cattcatgcc gccgcggtcg agcaggatct ggttctcctc gtccagcatt tcgatgcggt
     4621 tgcggtccgg cgggcgatcg acgccgtctt cagcggggag gcgaatgtga acgtcctgct
     4681 ctccaccgtg ggtgagccga cggacgccca acagaccatc gaacttaccg gtcgtccgat
     4741 cgcgcagggg gatggcgaag cagacgagtt ggtggcattg acgatcgtcg atgtgacgga
     4801 gcaggaacgt cttcgccagc ggcatgcgaa cgtcgagaat gtgaccagtc gcctgatatt
     4861 gacgaacgcg gaactcctca acgcgaacca ggatctctcc gcgacgatca gcagattgcg
     4921 cgcggagaac gaggagatga tggtcgccgc cgaggaaatc caggcggcca cggaagaggt
     4981 cgagacgctc aacgaggaac tccaggccag caacgaggaa ctggagaccc tcaacgaaga
     5041 gcttcaggcg acggtcgagg aactcaatac cacgaacgac gacttgcagg cccggacggt
     5101 cgaattacag acactcgcga tcgagggcga gatatctcgc cagcaattgc gggcgatcct
     5161 cgacgccctc gagatcgcga cgatcgtgat ggaccggcac ggctcgatcg tcatcgagag
     5221 cgaaacctac cggcgagtct tccagcgtca gagcgatcag tcgcgtggca tggtcgatga
     5281 ggaggggacc ccgctcccga tcggatccac gccggttcac cgggcgatga acggcgaggc
     5341 gttcatccag cgattcggca tctccagtgg cgaaggcacg acatggtacg tcgcgaactc
     5401 cgtcaacatc gacctcaccg attccgaccg cctgacgctg ctctctctcc gcgaggcgga
     5461 atagccccac ggcgtggccg gtgtgcgctc cgttcccgtc cccacggcgc ttgtcgccgt
     5521 ttcgggtcgc gagcgacgcc tatcccccgc tctccagcga ccaaagatcg agttgccatc
     5581 gatcgtgagg caggttgctg cactccgtcg tcccgcccca cgtacgaccg catgccccga
     5641 gccgcttccg tggagtgcgg agatcttccc agataactac cggcttcgac ctccgtcgtg
     5701 gacaccgcgg tcgtacgtac gggggacttc tatgtccccc ctttgaggaa caccaggccg
     5761 cattgcatta gcgaccgcgc gggtccggga cgacaccgag cgcactggca aggtcgatct
     5821 ggtgatcctg ctcctgcttg atgatctcct ggagaatctc ggcgagcgcg tattcaccca
     5881 gggcctcggc ttcccggatc cgcgttcgat agttctcgat cgttgtatcc tcggcccgca
     5941 agtccgccca gagcatcccc tcgttgtctt ccggaacctc gacgggttcc ggaacgtggg
     6001 tgggataggc cccgaagtag tcgagttgct tggccaccgc gatcgcgtga tccaactcct
     6061 cgtgcgcgtg aacttccagt tcccccgcga tacccatgta ggccgcgcca tcgagcttct
     6121 ggctgtagat cacgtactgg acgatggcct tgtattcgcg ggcaagatcc tgctgaagtc
     6181 cttcgatcaa gccttcccgt gtcacctgga tcggatcggt cgttgccatc tgctctctcc
     6241 ttctgtcgaa ctggacacca tgaaaacctg agggtccgga attcggacgc ctatgcggcg
     6301 tgagcgcgct cggccgccgc gatcacctcg atctggccgt cccggacccg cgtcgcgtac
     6361 gtcgtgagtg gcgacgtggc cggaccgttc acgacatgac cgtcggcgag cgcgaagaca
     6421 ctcccatgcc acgggcaggt caggcatccg tccttcaatt cgccttcgtc gagcgggcca
     6481 ccgaggtggg tgcaggtcgc ggatgccgcg agcacgcgct caccctggcg gacgaggacg
     6541 acggggtcac cctcgacgac gacccgcgtc ggggtcgcct cggggagatc ggcgacggcg
     6601 atcgccggtg cccacttccc ggttggctcc tcgaaagcag tgcgactgac cccggtaccg
     6661 agccgatagg aaagatcgcc accgagcgac gcggccagcg tggcaacgct tccacccgcg
     6721 agggaaaagg cgatcccggc cccacgatgc tgatggctgc gaagaaacca cgaggtcccg
     6781 tagagccacg tcgcaccgat gttgagcacg ccatgcagcg tcccgagccg ccgcggctga
     6841 tcctgatgct ggaggtcgta ccactgggcg atcccggtga ccgcggcggc gaatgcgctc
     6901 acggtcccgg ccttcaaggc gaaatcggcg gcatcctcca tcccgaccgc atcgaagacc
     6961 gcgctcgacg tccagaaccc gatcggcagg tccgtcaccg cgggatgcag gggatgaccg
     7021 agccaggtcc cgtagagcgc gtccttcagg gcgggcggcc ctttctcccc gaaaacgggg
     7081 gtcgacgcgg catgggcgac cccggcgacc ttgtcgagcc atggcgaggc atcgaccagt
     7141 ctgtcggcga gcgtacgaat cgaaaccatg acctctgctc cgtttccatg ccagccactg
     7201 agttcagcgg gagacatcgc gtgcggtgtc gtgaatctgc gggcaaaacg cccaactctg
     7261 cggcccgtga cgcatgtcga atgccaacac ggcaaacgcg gtcccgggtt gtgcaggggg
     7321 gtgggcgata tcggcccacg acgccgaagc cgaggacctg ccgttggtat cgcgttgcgg
     7381 attcgaccag gcgaggcggg cgggcgccgc gctcgggcat gagaatcccc cgacgacacc
     7441 acacgccggg ggattcaaaa agcaggaccg accggcggat gtattccggt cgatgcaaca
     7501 tactagtgag gcgagcggtc gcctttcaat ggaaacaccg atttccccga catcgtcgtt
     7561 cctattccgg gagatacatt ctccgcgatc gatccctgcg tactccctcc ggaccaggca
     7621 acacgacatc cccgatcggc atcttgtgca gtcagcgccg aggggtgaag atcgccggca
     7681 ggtgacgttg tcagccacca gacatcagtt cgaccattcg tcaaggatcg ccccgtgagt
     7741 ctcgttcaat ccgacctctc ccgcgccgtc gatatcctgc acgacctgat cgcgattccc
     7801 agcgtcaatc ccgcctacga tccccccagc gagggagaat ccgccgtcgc gacgtacgtc
     7861 gagtgctggg cacggtcgca cggactggcc gcgactcgcg gcgtggtcct gccgggacgc
     7921 gagaacgtca tcgttcgact cgaaaccagg ccgaacgccc cgattctcct cctcgaggcc
     7981 cacatggaca cggtctccgc cgacacgatg ccggatccct tcgcggcgac cgtccgcgac
     8041 ggccacctct acggtcgcgg cgcctgcgac accaagggat cgctcgccgc gatgatggcc
     8101 gccgtcgaat cactggtcgc catgcgtgac gagcttccat gcacggtcat cctgctcgcc
     8161 gccgtcgacg aggagacgag cggcaccggc gtccgcgcct gggtcgaggg cgggcactac
     8221 gccgatgcgg cgatcgtcgg tgaaccgacg cgcctgcgtg tgatccacgc ccacaacggc
     8281 gtcgtccgcg gcgcgatcgg cgtcagcggc agggccgcgc acacgtccgt cgcccacgaa
     8341 gggatcaacg cgatcgacgg catggccgag gtcgtgctgg cgctgcgcgg cctgaacgcg
     8401 gatctcgcgt cccggccagg cggcgccacg gccaacggtt ccttcacggt cagcatcatc
     8461 gcgggcggga ccgggatcaa catcgtgccg gagcagtgcg tcattcatta cgaccgccga
     8521 accgtccccg gcgatcaggc ggagaacgtc ctcgccggga tcgatgcgac ccttgatgcc
     8581 gtgcgaacgc agcgccccga cctcacgatc gtccgccacg atccgtccct gatcgacgac
     8641 gcactcgata cctccccggc ggaggcgatc gtcgtcgtcg cctcggaggc gaacgatgcg
     8701 ctcggcctcg atccagcgcc gacgaccgtg ccatacgggt ccgacggcag caagctcagc
     8761 gtcatcggcg gcatcccgac ggtggtctac ggtccgggcg atatcgcgca ggcgcacggc
     8821 gccgatgaat atgtcgagct atccgagctc accgcggccg cggccttcta tcgcgaggtc
     8881 gcgatgcgat tccgcggtca tcgccaggtt tccattcatg aatgaatcgc gataagatcc
     8941 gatcgagaag aaccgcgtcc cgccagcacg tcccatatca gtcatacccg gagattcccc
     9001 atgaccacga tcctgctcac cggcttcgaa cccttcggcg gcgacgcgac caatccatcg
     9061 tgggaggcgg tcaagcgtgt cgccgatgca tgggacggtc cggaggacct tcacatcgcc
     9121 gaactcccgg tcatctttca cgtcgccggg gatcgtcttc gcgacctgat gcgggaatac
     9181 cagccggatc tcgtgattgc gaccggcctc gccggaggac gcccccaggt gacgccggag
     9241 cggatcgcga tcaatgtcga cgacgcccgg atcccggaca acgccggcaa cgatccgatc
     9301 gacgccgcga tcgcggagga cggtcccgcg gcgtacttca gcacgctgcc gatcaaggcg
     9361 gcggtcgccg cgatccgcga gcaaggcatc ccggccgcgg tctcgaactc cgccggcacc
     9421 tatctctgca atcacatctt ctacgcgatc atgcacgagc aggcgcagca gtatcccggc
     9481 gcccgcggtg gcttcgtcca catcccctat tcgccggaga tggtcgccga tcgcccgcag
     9541 ccgagcctgg cactcgatct gaccgcgaag gcgatcgcga cgatcatccg cacctcgctc
     9601 gtcacctccg gtgacctcgc actggccggc ggcgcgatca gctagcagac gtatccgttg
     9661 gatcgagtca ccacaagggt gtcccgtcca gatatcgtat cttcatgacc cgcggcgttg
     9721 gtccatacgg gaaacgccat gcccggagtg gggcaggcgg gacaggcagt ccggact
//
LOCUS       DASTMD010000593         3645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2913857, whole genome shotgun sequence.
ACCESSION   DASTMD010000593 DASTMD010000000
VERSION     DASTMD010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2913857"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1114
                     /locus_tag="VFQ54_08740"
     CDS             143..1114
                     /locus_tag="VFQ54_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165119.1"
                     /translation="MVKVRTSVRECRGAVRLFPWITAALLALMMLVTASSGGSALASP
                     AATPVAPSNPTWSIDIGYSGYDQMHLDNPVTLTVTIRGSGLAQNQLLNFTTSLPASVQ
                     SLVTGTSCTPTVAGSTCSFGSGGFSGASADTGSSVNGVVAPAASGNAGAVVGFTISGL
                     DFLDPLLGAAACLHVDGPDGVTTTCSDILVVPADAPTVAPTIVTVDPTVAATTGPTDP
                     LVTPTTGPVDPLVTPTSGPVDPLITPTTAPGATGAVPNGTPEPVTGNARPGNTGGGSS
                     STSGVVGLPNTGTASTRHDDTLSYILIGVSLILVISAAGVALNRRQN"
     gene            1314..2525
                     /locus_tag="VFQ54_08745"
     CDS             1314..2525
                     /locus_tag="VFQ54_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0165120.1"
                     /translation="MSTPKITHVQTAAIRCVGPSVLVKIWAGDIYGIGECYPSGPAAA
                     IHQIIHSLADEITGEDPRNVERLAEKLRLWNIFTGGQGGSITTAISGMEMALWDLAGK
                     LQGVPVYRLLGGAFRTSIRLYADCNAGTVDAAAHHVYEVRDGALVADDEAESLAAATK
                     AEVDRGFTALKFDIDDITGPMHRDYWNRSASTAEIAIMVNKVEAVRNAVGPGIDVAVD
                     MHGRYDVPTAIRVAQALEPYDLMWLEEPVPPENIQALAEVRQSTSTPICAGENLYTRF
                     QFQPLLAAHAVDVVMPDLAKCGGLAEGKRIANMAEMDYIPFSPHNVSSPIGTIAMAHV
                     CAAVSNFTVLEWHAADLPYWKDFITWSGGEIIVDGAIQLTEAPGLGLELDEDVAFEYR
                     HTKGGIAFFDR"
     gene            2605..3276
                     /locus_tag="VFQ54_08750"
     CDS             2605..3276
                     /locus_tag="VFQ54_08750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0165121.1"
                     /translation="MSDPPVAQQESRQPATGITIELITRRRIGEAGDVIARSLLVEPG
                     LRALLEDEQARYRMMKPLMTGTVRNAARHNSCYVALQAGKVVGVAVWLPPGAFPFDNR
                     TNLRMIPALRGFIGLGRATMNQFAEMEMNAEKHFPQEPVWYLQALGVAPGFQGKGIGS
                     QLIVPALERADREQLPCYLETGTEANVRFYKRAGFAVREAGIHLAPLEDGPTHWTMMR
                     PRPKP"
BASE COUNT          655 a         1244 c         1116 g          630 t
ORIGIN      
        1 catgcacaga ttgtccacat gctcataatt ttacccgatt aggatagtac taatgggtga
       61 caaatcctga tacgtctgta taatcgggta tagggactcg ttctactcat gactatcgga
      121 tacatgacca taggagtttt ggatggtcaa ggtgaggaca tctgttcggg aatgccgggg
      181 tgcggtccgg ctcttcccat ggatcaccgc cgcgcttctc gcgctgatga tgctcgtgac
      241 ggcctcctcc gggggaagcg ccctcgcatc cccggcggcg acacccgttg ccccgagcaa
      301 tcccacctgg tcgatcgata tcggctatag cggatacgat cagatgcatc tcgacaatcc
      361 ggtcacgctg accgtcacga ttcgcggaag tggtctcgcc cagaaccagc tcctcaactt
      421 cacgacatcg cttcccgcca gcgtgcagtc actggtcacg ggaacctcgt gcacacccac
      481 ggtcgcgggc tcgacgtgct cgttcgggag cgggggattc tccggtgcca gtgccgacac
      541 ggggtcgtcg gtcaatggcg tggtcgctcc ggcggccagc ggcaatgccg gagcggtcgt
      601 cggcttcacg atctcgggtc tcgacttcct cgatcctctg ctcggagccg cggcctgcct
      661 ccacgtggac ggtccggatg gcgtcacgac aacctgctcc gacatcctgg tcgtgccggc
      721 cgacgccccg accgtggcgc cgacgatcgt aaccgtggat ccaaccgttg ccgcgaccac
      781 cggcccgact gatccgctcg tcacaccgac gaccggtccg gtcgatccgc tggtgacgcc
      841 gaccagcggc cccgttgatc cactcatcac accgaccacc gctcccggcg cgaccggagc
      901 cgtgccgaac ggcacgccgg aacccgtcac gggcaacgcg cgtccgggca ataccggcgg
      961 cggatcgtcg tcaacgtccg gcgtcgtcgg gcttcccaat accggaaccg cctccaccag
     1021 acacgacgat acgctctcct acatcctgat cggtgtgagc ctcatcctcg tcatttccgc
     1081 ggccggcgtg gcactcaacc gacgccagaa ctgaccgcga tcgcaatcct gacgacggaa
     1141 ccccgcttca catggagcgg ggttctgccg tagcccctcc gatgaggggg aagccttcct
     1201 gccgacatca tgtcgcggcg cattgaccgc ggtggtgccg cttgcgatga ttgccatcta
     1261 gctcacagtt ccgacctcag gccgttcgac atccgttcga aggagcacgc ttcgtgtcca
     1321 cgccgaagat tacccacgtc cagaccgccg cgatccgctg cgttgggccc tccgtcctcg
     1381 tgaagatctg ggccggtgat atctacggta tcggcgagtg ctacccatcc gggcccgccg
     1441 ccgcgatcca ccagatcatc cactcactcg ccgacgagat caccggcgag gatccacgaa
     1501 acgtcgagcg gctggccgag aagctccgcc tctggaacat cttcaccggc ggtcagggcg
     1561 gatcgatcac caccgcgatt tccggcatgg agatggcact ctgggatctc gccggcaagc
     1621 tgcagggtgt cccggtctac cgcctcctcg gtggtgcgtt ccgcaccagc atccgcctct
     1681 atgccgactg caatgccggc accgtcgatg ccgccgccca ccacgtctac gaggtgcgcg
     1741 atggcgcgct cgtcgccgac gacgaggcgg agagcctggc cgcggcgacg aaggcggagg
     1801 tcgatcgcgg cttcacggca ctgaagttcg acatcgacga catcaccggc ccgatgcacc
     1861 gcgactactg gaaccggagc gccagcaccg ccgagatcgc gatcatggtg aacaaggtcg
     1921 aggcggttcg caacgcggtc ggacccggga tcgatgtcgc cgtcgacatg cacggtcgct
     1981 acgacgtccc gacggcgatc cgggtcgcgc aggcgctcga accctacgac ctgatgtggc
     2041 tcgaggagcc ggttccgccc gaaaacatcc aggccctggc cgaagttcgg cagagcacga
     2101 gcacgccgat ctgcgccggc gagaacctct acacccgctt ccagttccag ccgctgctcg
     2161 ccgcccacgc cgtggacgtg gtgatgccgg atctcgcgaa gtgcggcggt ctcgccgagg
     2221 gcaagcggat cgcgaacatg gcggagatgg actacatccc cttctcgccg cacaacgtct
     2281 cgagcccgat cgggacgatt gcgatggcgc acgtctgcgc ggcggtctcg aacttcacgg
     2341 tgctggagtg gcatgccgcc gatcttccct actggaagga tttcatcacc tggtccggtg
     2401 gcgagatcat cgtcgatggc gcgatccaac tcaccgaagc gccgggtctc ggcctggagc
     2461 tggacgagga cgtggcgttc gaatatcgcc acaccaaggg gggcatcgcc ttcttcgacc
     2521 ggtaggaacg tcgtccggcc agcgcgagag gcgggatgac gggcagcgcg accggtcgta
     2581 tgctgacgat cggaggaatc gttcatgtcc gatccacccg tcgcgcaaca ggaatcccgt
     2641 caacccgcta ccggtatcac catcgaactc atcaccaggc gccggatcgg ggaggcgggc
     2701 gacgtcatcg cccgcagcct gctcgtcgag cccggtctcc gcgcgctcct cgaggacgag
     2761 caagcgcgct acaggatgat gaagccgttg atgacgggga ccgtgcgcaa cgcggcgcga
     2821 cataactcat gctatgtcgc gcttcaggcg ggcaaggtcg tcggggtggc ggtctggctg
     2881 ccgccgggcg cgttcccctt cgacaaccgg acgaacctgc ggatgattcc cgctctgcgc
     2941 ggcttcatcg ggctcggtcg cgcgacgatg aaccagttcg ccgagatgga gatgaacgcc
     3001 gagaagcatt tcccgcagga gccggtctgg tatctccagg cgttgggtgt cgcacccggg
     3061 ttccagggga aggggattgg atcgcaactg atcgtacccg ccctcgaacg cgccgaccgc
     3121 gagcagttgc cctgctatct cgagacggga acggaggcga atgtccgctt ctacaaacgc
     3181 gcggggttcg cggttcgcga ggcggggatc cacctcgccc cgctggagga cggcccgacg
     3241 cactggacga tgatgcgccc gcgcccgaaa ccgtaacgac cgcgtcacca gtgaccgttc
     3301 accgtagcga gtcagctggt ctgacccgac agcgcaccga tcgaacgaag ttcgatatga
     3361 tgtcggcctg aacagtgccc caccatgctc ccgacctccc attgaactgt catttcgagc
     3421 ttgccgagaa atccccacgc gaagcgcagt cggccaatgg ctgacatgat gccgtgatcg
     3481 gcgcggcatc gccaatcacg atccattcgt ggacgatcgc gcgtgcagag cgccacgaat
     3541 ggcgtgatcc cgttgcggcg ggacatcccg gcaccggacc gaattcgctt cccaccactc
     3601 ccgttgccat caccgccttg cgtgtttcac cttcccattc acaac
//
LOCUS       DASTMD010000594         3735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2288267, whole genome shotgun sequence.
ACCESSION   DASTMD010000594 DASTMD010000000
VERSION     DASTMD010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2288267"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFQ54_08755"
     CDS             complement(<1..700)
                     /locus_tag="VFQ54_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU0165122.1"
                     /translation="MPEAIESWTESFRDVVENNNVEDAIRILAQFRTGHAGTPPQAIK
                     ARAIGIIEKLTVGDLERRLAVAYGFVRSEDDGAKEIGISLLSACYGLAPAEVAGWMRR
                     IGDDPNWEVREWAGTALGRTIPAQFADVLPRLRVWAADDSANVRRLVVVAIGYATKAC
                     SPDETLQLLTLLTPLMADRDPYVGKNLGAYAIGSYAIRHHPVVVASWTATLDLAEEQT
                     AWNLAMTFTTGGAPG"
     gene            1025..>3735
                     /locus_tag="VFQ54_08760"
     CDS             1025..>3735
                     /locus_tag="VFQ54_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165123.1"
                     /translation="MQFFAAIMLLAQTFSSAALTLASAPSASGQTGIVAQDSSPEATV
                     EPTQTLTPTATTAPPVPTQMPPTNTSVPPTATNIPPTNTAVPPTATNVPPTNTALPPT
                     ATNVPPTNTSIPPTATYTMAPTATDTPVPSSGDFTVVGDKTTVDPGETVTFTVHVQGK
                     LETAGTMALSIYFPITARTGGDLSALAGCAQAKNAYQSLVSRWQLWLNCPTAPDGSFD
                     YSFSISGPALTTVGSTSVYGGIYEGSTNGPQLAWHSPAAPLTVVGPTNTVTVAPSPTL
                     SPTATNAPPTATQVPPTNTATATSTPSITPTATKTPTATPTGTPTTASVGSSVTITTD
                     TPTIDINGSASYTVHFWGAIPSHGTVDLDAYFPIVAHTGGEFGAVAGCSKPYDQDLGG
                     SGGGNWNIELNCPTAPNGSFDFTYTVIGTISSFGTFFSHATLYTPGYGQTVLANLPSA
                     AAVNVVDPTLDVTITTDTPSVDVGSSIRYTVHFAGTLSSQGNVEMDAYFPITNRTDAD
                     FSAMSACGDPSYDTVIDDDWNVEFICPSDSDGSFDYTYTITGTAPGTVGTVDSHATLY
                     LPDSSVDLPSVAPVTVNGVDATGNISISLDPSTADAQAGGTITNTIHISGSIPAPGVV
                     ELDAVLPILNYTDDNVGTIPACSIDIEGSAGDGLYDVRILCSTGPDGSFDYTFTVSGD
                     TPAQPGSYDASATLSTPTYGDFVIATADPAATLTVTGVADVSNITMTTDQPYYHVGDA
                     ATFSVTMNGKLAVPGSYRVNLTIPLSALTAENLDPDSSSCVKYDGYSSGPWYVYQLSC
                     QTDAEGNFDYSFSLNGTIATTASVYGSNYSLIREDGLTLQSGTVDILVLAPGDSADNI
                     TFNIGSTPGDPQPGGTSIVSNVSVYGSMFDPDGTNIPLRLVIQIPIA"
BASE COUNT          743 a         1361 c          979 g          652 t
ORIGIN      
        1 gaccgggggc acctcccgtc gtgaaggtca tcgcgaggtt ccacgccgtc tgctcctccg
       61 cgagatcgag cgtcgccgtc caggaggcga ccaccacagg gtgatgccgg atcgcatagc
      121 tgccgatcgc atacgcgccg agattcttcc cgacgtacgg atcgcgatcc gccatcagcg
      181 gtgtcaggag cgtcaggagc tgtaatgtct cgtcaggcga gcacgccttc gtcgcatagc
      241 cgatcgcgac gacgacgagg cgtcggacat tcgccgaatc atcggcagcc cagacgcgca
      301 ggcgaggcag gacgtccgcg aattgcgccg gaatcgtccg ccccagtgca gtgccagccc
      361 attcccggac ctcccagttg ggatcgtcgc caatccgtcg catccagccg gcgacctcgg
      421 ccggggcgag cccgtaacag gctgacagga gggagatccc gatctccttc gcgccgtcgt
      481 cttccgaccg cacgaatccg tatgccacag cgaggcgtcg ctccagatcg ccgacggtca
      541 atttctcgat gattccgatc gcacgcgcct tgatcgcctg cggcggcgtc ccggcatgac
      601 cggttcgaaa ctgcgccaga atccggatcg cgtcttcgac attgttgttc tcgacaacat
      661 cccgaaacga ctcggtccag ctctcgatcg cctcaggcat ccaccgccga cctttcgtca
      721 ctgcgatctc ctgtccacat cggccggtca ccacgagcca caccatccgc gaggatccag
      781 ccaggatctt atcgccctcc ccgacaggca tcacgaccga atatgacaca aagtgtgtac
      841 aaacaatgca agcatcacgt actatgcgcg tgggtcacta ggtacagaat tggggagcgt
      901 tatgcccaac cccggtcgct catcgtgctc acggcaccga tgaacgtcgg tcggtgccgg
      961 tcgcgcgatg acaagtgtgt ggggcgggga aatgcaacat tcaaagtcac gagtgcggcg
     1021 cgcagtgcag ttttttgccg cgatcatgct gctcgcgcag acattctcga gcgcggcgct
     1081 tacgcttgcc agcgcgccgt ctgccagcgg ccagaccggc atcgtcgcgc aggattcgtc
     1141 acctgaagcg accgtcgagc caacccagac tctcactccc acggccacaa cggctccccc
     1201 tgttcccacg caaatgcctc cgacgaatac gtcggttccc cccaccgcca cgaatattcc
     1261 cccgacgaac accgcggtcc cacccaccgc caccaacgtt cccccgacga acaccgcgct
     1321 cccgcccacc gccaccaacg ttcctccgac gaatacgtcg attcctccga cagcaaccta
     1381 cacgatggct cccacggcaa ccgatactcc cgtaccaagc tccggcgact tcaccgtcgt
     1441 gggagacaag acgacggtcg atccgggcga gaccgtcacc tttaccgtcc acgttcaggg
     1501 aaaactcgag accgccggga ccatggcgct ctcgatctac ttcccgatca ccgcgcgcac
     1561 cggcggtgat ctttctgcgc tcgccggttg cgcgcaggcg aagaacgcgt atcagagcct
     1621 cgtcagccgc tggcagctct ggttaaactg ccccaccgct cccgatggca gcttcgacta
     1681 ttccttcagc atcagcggtc ccgcgctgac cacggtcgga tcaacctcgg tctacggcgg
     1741 catctacgag ggaagcacca acggtcctca gctcgcgtgg cacagcccgg ccgcccccct
     1801 gaccgtggtc ggaccgacga acacggtcac cgtggcgccg agtccgaccc tctccccgac
     1861 cgccaccaat gccccaccaa ccgctacgca ggttccacca accaacaccg cgaccgcaac
     1921 ttcgacgcct tcgatcacgc ccacggcgac gaaaacccca acagccacac ccacaggaac
     1981 gccgacgacg gcctcagtgg gttcttcagt cacgatcacc accgacacac cgacgatcga
     2041 tatcaacgga tcggcgagct acacggtcca cttctgggga gcgattccga gccacggcac
     2101 cgtcgatctc gacgcctact tcccgatcgt cgcgcacacc ggtggcgaat tcggcgccgt
     2161 cgccggatgc agcaagccat acgaccagga tctcggtggc tccggcggcg gaaactggaa
     2221 catcgagctg aattgcccca cggcgcccaa cggcagcttc gacttcacct ataccgtgat
     2281 cgggacgatc tcgtccttcg gaacgttctt ctcccacgcc acgctctaca ctccgggcta
     2341 tgggcaaacc gttctcgcaa atctcccttc cgccgccgcc gtcaacgtcg tcgatccgac
     2401 cctggacgtc acgatcacga ccgatacgcc gtcagtcgat gtcggaagct ccattcgcta
     2461 cacagttcac ttcgcgggca ccctgtcgag ccagggcaac gtggaaatgg acgcctactt
     2521 cccgatcaca aaccgcaccg acgccgattt cagtgcgatg tcggcctgtg gcgatccaag
     2581 ctacgacacg gtcattgatg atgactggaa cgtcgaattc atttgtcctt cagattccga
     2641 tggcagcttc gactacacct acacgatcac cggcacggct cccggaaccg tcggcacggt
     2701 cgattcccac gcgacgctct acctgccgga ctccagcgtc gatcttccgt ccgtcgcgcc
     2761 agtcaccgtc aatggcgtcg acgcaaccgg caacatcagc atctcgctgg atccatcaac
     2821 cgcggatgcc caggccggcg gcacgatcac gaatacgatc cacatcagcg ggtcgatacc
     2881 ggcgccggga gtggtggaac tggatgccgt actgccaatc ctgaattaca ccgacgacaa
     2941 cgtaggcacc atccccgcgt gctccattga catcgaggga tccgcaggcg acggtctcta
     3001 tgacgtccgg atcctttgct ctaccggacc agacggatcc ttcgactaca ccttcaccgt
     3061 cagtggcgac acgcccgcgc aacccgggtc atatgacgcc tccgcgaccc tgtcgacacc
     3121 gacgtacggc gacttcgtca tcgccaccgc ggacccggcc gcaacactca ccgtcaccgg
     3181 cgttgctgac gtctccaaca tcacgatgac cacagaccag ccgtactacc acgtcgggga
     3241 tgcggccaca ttctccgtca ccatgaacgg caaactcgcg gttcccggta gctaccgggt
     3301 caatctgacc atccccctga gcgccctcac cgccgagaat ctcgatccgg acagttcgtc
     3361 gtgtgtgaaa tatgacggct attcctccgg accctggtac gtgtatcagc tctcctgcca
     3421 gaccgatgcc gagggcaatt tcgactactc gttcagcctg aacggcacca tcgcgaccac
     3481 tgcgtccgtg tacggcagca actactcgct gatccgggag gacggcctga cgcttcaatc
     3541 cggcacggtg gatattctcg tgctggcccc gggtgattcc gcggacaaca tcaccttcaa
     3601 catcggctcc accccgggcg atccccaacc aggggggacg tccatcgtct cgaatgtctc
     3661 cgtttacggt tccatgtttg atcccgacgg cacgaacatc ccgcttcgcc ttgtcatcca
     3721 gatcccgatc gctat
//
LOCUS       DASTMD010000595         1626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1127527, whole genome shotgun sequence.
ACCESSION   DASTMD010000595 DASTMD010000000
VERSION     DASTMD010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127527"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1273)
                     /locus_tag="VFQ54_08765"
     CDS             complement(29..1273)
                     /locus_tag="VFQ54_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU0165124.1"
                     /translation="MSATAWPEGRDRHERLEDVLRTDGLDLLVALTPENAEYLAGRNS
                     YIATNWRIPGIFGVVAGPDGKRAIVDSDSSFDPRNPGPYPVSVYESWLETLDLRDARG
                     ASIRERAVAQRPHLVARPAQFNLEKITAAVVAAIHRVNEDPRRIGLDLEHIDVASFHR
                     LKAQLPYIELVDASQRFYDLRAIKDADEIAKLRLAAELTTIGIRGAIDALRLGQSSAT
                     VNANYHIAVNTAVASDPRFAGFRQAEGLATIGMSTPSAGTVAPGETIRFDMQVDMSGY
                     HSDIGRTVIHRPTDDQRVVYEVLRTALFTAQELVKPGVTFAEIFAAGTASMLDAGFTT
                     FSRGHLGHSDGLTQNFEEAPFIAPGESRPLVAGMVLSLELPYYLRGTGGFQLERMLVV
                     TEDGHETLDQLPFDLEIDLDRS"
     gene            complement(1270..>1626)
                     /locus_tag="VFQ54_08770"
     CDS             complement(1270..>1626)
                     /locus_tag="VFQ54_08770"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU0165125.1"
                     /translation="FGPRIQRSTLASEAIFLMQREALGALGYRRLEWKCNNANARSKR
                     AAERFGFTFEGIFRQHSVVKGKNRDTAWFSLLDSEWPANERAFEAWLSPENFDAAGNQ
                     RRSLAEIRADLTATNV"
BASE COUNT          277 a          544 c          521 g          284 t
ORIGIN      
        1 tactatgtcc cgcctcgaga gtggctcgtc agctccggtc gaggtcgatc tccaggtcga
       61 acgggagctg gtcgagcgtc tcgtgcccgt cctcggtcac gacgagcatc cgttcgagct
      121 gaaagccgcc ggtcccgcgc aggtaataag gaagctccag actcagcacc atccccgcaa
      181 cgagcggccg cgactcaccg ggcgcgatga atggcgcctc ctcgaaattc tgggtcaggc
      241 catcgctatg cccaagatgc ccgcgactga atgtcgtgaa tcccgcatcg agcatcgacg
      301 ccgtcccggc ggcaaagatc tccgcgaagg tgacgcccgg tttcaccagc tcctgagcgg
      361 tgaagagcgc ggtccgaagc acctcgtaga caacgcgctg gtcgtccgtc ggtcgatgga
      421 tcacggtgcg gccgatgtcg ctgtgatagc cgctcatgtc gacctgcatg tcgaagcgga
      481 tcgtctcacc gggcgccacc gtcccggccg acggcgtcga cattccgatc gtcgcgagcc
      541 cttccgcctg gcggaatccg gcgaatcgag gatcgctggc gaccgcggtg ttgacggcga
      601 tgtggtagtt ggcattgacc gtcgccgagg attgcccgag gcggagggcg tctatcgcgc
      661 cacggattcc gatcgtcgtc agctcggcgg caaggcgaag cttcgcgatc tcgtccgcat
      721 ccttgatcgc gcgcaggtcg tagaaacgct gggacgcatc gacgagctcg atgtacggca
      781 actgcgcctt cagccggtga aagctggcga cgtcgatgtg ctcgagatcg agtccgatcc
      841 ggcgcggatc ctcgttcacc cgatggatcg ctgccacgac cgcggcggtg atcttctcga
      901 gattgaactg ggctggtcgc gcgaccagat gcggccgctg ggcaacggca cgctcccgga
      961 tcgaggctcc gcgagcatcg cgaagatcga gcgtctccag ccagctttcg tagacgctca
     1021 ccggataggg accgggattt cgcgggtcga agctcgagtc gctatcgacg atcgcgcgct
     1081 tcccgtctgg cccggccacg acgccgaaga tcccgggaat ccgccagttc gtcgcgatgt
     1141 agctgttgcg gcccgccagg tactccgcgt tctccggagt cagggcgacg aggagatcga
     1201 gcccatccgt ccgcagcacg tcctcgagtc gctcgtgacg atcgcgtccc tcgggccagg
     1261 ccgtcgcgct catacgttgg ttgccgtgag atcggcgcga atctccgcga gcgagcgccg
     1321 ctgattgccg gcggcatcga agttctccgg cgacagccac gcctcgaagg cccgctcatt
     1381 cgccggccac tccgagtcga gcagcgagaa ccaggcggta tcgcggttct tgcccttcac
     1441 cacgctatgc tgacggaaga tcccctcgaa ggtgaagccg aaccgctcgg ccgctcgctt
     1501 cgagcgcgca ttcgcgttgt tgcacttcca ctcgagacgg cgatacccca gcgcaccaag
     1561 tgcctcgcgc tgcatcagga agatcgcctc gctggccagc gtcgaacgct ggatccgcgg
     1621 gccgaa
//
LOCUS       DASTMD010000596         1564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_412573, whole genome shotgun sequence.
ACCESSION   DASTMD010000596 DASTMD010000000
VERSION     DASTMD010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412573"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /gene="ureG"
                     /locus_tag="VFQ54_08775"
     CDS             complement(<1..423)
                     /gene="ureG"
                     /locus_tag="VFQ54_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEU0165126.1"
                     /translation="MERPVRIGIGGPVGTGKTALVWRLCEALRDRVDLAVITNDIYTL
                     EDADFLVRNSALTPDRIIGVETGGCPHSAIRDDPSMNFDAVRELEMRHPSLQLILIES
                     GGDNLSATFSPELADASVFVIDVSGGDKVPRKGGPGISR"
     gene            complement(456..1151)
                     /locus_tag="VFQ54_08780"
     CDS             complement(456..1151)
                     /locus_tag="VFQ54_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEU0165127.1"
                     /translation="MTGSLGLLRLLQIADSAFPTGGYALSHGLEGLHALDLVESTEDV
                     TNFAVVHIAETLERLELPLVRHAHQRAGESDIAGLQELDALTSAYKPVAAFRRASLNV
                     GRRLLEAASPLLAPDCAATSYRTSTHTDERSGHHAVAWGVVCQGIGVGMREAVLAYGA
                     TSLSGYVAAAIRLGTIGQRAGQAIVAGLHDRLLVAVEASISLDLDDLGGYSPAIDITG
                     MRQSSLQGRLFTT"
     gene            complement(1151..>1564)
                     /locus_tag="VFQ54_08785"
     CDS             complement(1151..>1564)
                     /locus_tag="VFQ54_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEU0165128.1"
                     /translation="MADLVIWKPEFFGVRPELIVKGGMIAWAAMGDANASIPTPQPVL
                     MRPMFGADVPEWTSLTFVSQASLAAGVPDELGLRKTAVAVRDTRSVRKQDLIHNGATP
                     EITVDPETYEVRADGVLLVSEPASELPMAQRYFPF"
BASE COUNT          282 a          523 c          491 g          268 t
ORIGIN      
        1 gcgtgagatc ccgggtccgc ccttccgagg gaccttgtcg cctccggaga cgtcgatcac
       61 gaagacggaa gcgtcggcca gttctggact gaaggtcgcc gagaggttat cgccaccact
      121 ttcgatcagg atcagttgca gggaaggatg tcgcatctcg agctctcgca cggcgtcgaa
      181 gttcatcgac ggatcgtccc ggattgccga gtgcgggcat ccgccggtct cgacgccgat
      241 gatccggtcc ggggtgagtg ccgaattgcg gacgagaaag tcggcatcct ccagcgtgta
      301 tatgtcgttg gtgatcacgg ccagatcgac ccggtcgcga agcgcctcgc agagtcgcca
      361 cacaagcgcc gtcttccccg tccccactgg tccgccgatg ccgatccgta ctggtcgctc
      421 catcttgtat ctcctgatct ttccgcatcc ggacatcacg tcgtgaagag tctgccctgc
      481 aatgacgact ggcgcatacc ggtgatgtcg atcgcggggg aatatccgcc gaggtcgtcg
      541 aggtcgagcg agatcgacgc ctcaaccgcc acaagcagcc gatcgtgcaa accggcgacg
      601 atcgcctgtc cggcgcgctg cccgatcgtg ccgagccgga ttgccgccgc gacgtagccg
      661 ctcaggctcg tggccccata ggcgaggacc gcctcgcgca tgccgacccc gattccctga
      721 cagacgactc cccaggcgac ggcgtgatgt ccggatcgct catcggtgtg cgtcgacgtc
      781 cgataggagg tggcggcaca atccggagca agcagcggtg acgccgcctc gagaagtcgt
      841 cgccccacat tcagactcgc ccgccgaaac gcggcgaccg gcttgtaggc agaggtcaac
      901 gcatcgagtt cctggagacc cgctatgtcg ctctcgccgg cgcgctggtg agcgtgccga
      961 accagcggca gctccagcct ctccagcgtc tcggcgatat ggaccaccgc gaagttcgtc
     1021 acgtcttccg tggactcgac gaggtccagc gcatggagtc cttccagtcc gtgcgagagc
     1081 gcatacccgc cggtgggaaa tgcgctatcg gcgatctgga gcagtcggag cagaccaagg
     1141 gacccggtca tcagaaggga aagtaacgct gtgccatcgg cagctcggac gccggttcgg
     1201 acaccagcag gacgccgtcc gcacgcacct cgtaggtctc gggatcgacg gtgatctccg
     1261 gcgtcgcccc gttgtggatc aggtcctgct tccgcacaga tcgcgtgtcg cgcaccgcca
     1321 ccgccgtctt ccgcagcccg agttcgtccg gcacgccggc ggcgagcgat gcctgggaga
     1381 cgaacgtcag cgatgtccac tccggaacat cggcaccgaa catcgggcgc atcaagacgg
     1441 gttgcggcgt agggatactc gcgttcgcat cacccatcgc ggcccaggcg atcatcccac
     1501 ccttgacgat cagttccggc cgtacgccga agaactcggg tttccagatc acgagatcgg
     1561 ccat
//
LOCUS       DASTMD010000597         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_679994, whole genome shotgun sequence.
ACCESSION   DASTMD010000597 DASTMD010000000
VERSION     DASTMD010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679994"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VFQ54_08790"
     CDS             complement(<1..825)
                     /locus_tag="VFQ54_08790"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEU0165129.1"
                     /translation="MTKTRVKSGLEAFLAGAGNEIDGMRVGLVTNPNGVDSRFRSTID
                     LLGASPRLQLVSLFGPEHGVRGDAQAGDHVAQAVDGRTGLPVHSIYGETRRPTPEMLA
                     GIEAIAIDLQDLGVRYATYLSTQIEVQAAAHDAGIDVVILDRPNPLADLRLEGNTLDP
                     TFTSFVGSYPIPVRHGMSLGELGLMIAAERGWKAPIVVRADGWRRTSWYDETGLPWVP
                     PSPNMPTLDGLILYGGTCLAEGTSWSEGRGTTRPFEYIGAPGIDAQALADDLTSRAI"
     gene            complement(837..1544)
                     /locus_tag="VFQ54_08795"
     CDS             complement(837..1544)
                     /locus_tag="VFQ54_08795"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU0165130.1"
                     /translation="MTDQPCIMVVGAHAADAEIMAGATVLKHVDAGWRAVIVHATLGE
                     KGSRTLSPEEYSAVKTGEAQEAARRLGADCVILPYLDGELPISEDVQWHIADAIRKYK
                     PTVLITHWKGSLHTDHVNTYENVRSSLFFAGLKTFKREYPNHYPRQILYAENWEDVEE
                     FVPDFYLDVSEVWDRYIEAIHAYGLFRGEASFPYEQWYRGASEMRGAEVGYARAVALM
                     KTETVYQRRRKIQLLGG"
     gene            complement(1612..>2567)
                     /locus_tag="VFQ54_08800"
     CDS             complement(1612..>2567)
                     /locus_tag="VFQ54_08800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0165131.1"
                     /translation="PSPAVTIRPYQRGDEASLVPVWNRALWSDPITFETWRAKVLLDP
                     NFDPATCLVADVDGVVRGFVLALTRQVPFFNEGLDPTTGWITAFGVDPDFQRQGIGRA
                     LLHGVLNQLRELGRAKIMLGPYVPNYFTPGVDVNAYAPAIGFFEKHGFRVTMRPLSMR
                     AILTGFTVPPALAPAYQKLEQYGIDIRPARPDDITPVLQFVRDHFSWDWHREATGVFQ
                     DLFAGLGDPRQVGLMVADKDGEIVGYSQHRGERFGPFGVREDRRSQGIGRVLLAEILL
                     TMRAKNFHTAWFLWTSDQAARLYAQCGFEEARRFAVMVRDL"
BASE COUNT          449 a          820 c          850 g          448 t
ORIGIN      
        1 aatcgcccgc gacgtgagat cgtccgcgag tgcctgggcg tcgattccgg gcgcgccaat
       61 gtattcgaac ggtcgcgtcg tcccgcgtcc ttcggaccag gaggttccct cggcaagaca
      121 ggtcccgccg taaaggatca gtccgtcgag cgtcggcata ttcggggacg ggggaaccca
      181 cgggagcccg gtctcgtcgt accacgaggt ccggcgccag ccgtctgcgc ggaccacgat
      241 cggcgccttc cagccgcgct ccgccgcgat catcaggccc agctcgccga gcgacatccc
      301 gtgacgcacg ggaatcgggt aggagccgac gaacgacgtg aaggtcggat cgagcgtgtt
      361 cccctcgagc cggagatcgg cgagcgggtt cggccggtcg aggatgacga cgtcgatccc
      421 ggcatcgtgc gccgccgcct ggacctcgat ctgcgtcgag agataggttg cgtaccggac
      481 accgaggtcc tggagatcga tcgcgatcgc ctcgattccg gcgagcatct ccggtgtcgg
      541 gcggcgggtt tcaccataga tgctgtggac cggcagaccg gtccggccgt cgaccgcttg
      601 cgcgacgtgg tcgcccgcct gggcgtcacc tcgaacccca tgttcgggcc cgaagagact
      661 cacgagctgc aggcgtgggc tcgcgccgag aaggtcgatc gtgctgcgga accgggaatc
      721 gacgccgttc gggttcgtca cgagcccgac tctcatgccg tcgatctcat ttcccgcacc
      781 ggcgagaaat gcctcgagcc cgctcttgac tctggtcttt gtcatggcga tcacggctat
      841 ccgcccagca actgaatctt gcggcgtcgc tgatagaccg tttcggtctt catcaacgcg
      901 acggcgcggg catatccaac ctcggcgcca cgcatctcgc tggcgccgcg ataccactgc
      961 tcgtagggga aggacgcctc gccccggaag agcccatagg cgtgaatcgc ctcgatgtag
     1021 cggtcccaga cctccgacac gtcgaggtag aagtcgggga cgaactcctc gacatcctcc
     1081 cagttctcgg catagagaat ctggcgggga tagtgattgg gatactcgcg cttgaaggtc
     1141 ttgagcccgg cgaagaagag cgacgaccgc acgttttcat acgtgttgac gtgatctgtg
     1201 tggaggctcc ccttccagtg cgtgatcagc acggtcggct tgtacttgcg gatggcgtcg
     1261 gcgatgtgcc attgcacgtc ctcgctgatc ggcagttccc cgtcgagata gggaaggatc
     1321 acgcagtcgg caccgagacg gcgggccgct tcctgggcct ccccggtctt gacggccgag
     1381 tactcctcgg gcgaaagcgt gcgcgatccc ttctcgccca gcgtggcgtg gacgatcacg
     1441 gcgcgccagc cggcgtcgac atgcttgaga acggttgctc cggccatgat ctccgcgtcg
     1501 gcggcgtgcg ccccgacgac catgatgcac ggttggtcgg tcatgatgat tcctgcttgt
     1561 ggttcccacc cggcgagaac gccagatgcg gaagatagcc attcggtaga gctagagatc
     1621 ccgcaccatc acggcgaaac gacgcgcttc ctcgaatccg cactgggcgt aaagccgggc
     1681 ggcctggtcg ctggtccaga ggaaccaggc ggtatggaag ttcttcgccc gcatcgtcag
     1741 caggatctcg gcaagcaaga cgcgcccgat cccctggctc cggcgatcct cgcgaacacc
     1801 gaacggcccg aatcgctctc cgcggtgctg cgagtagccg acgatctcgc catccttgtc
     1861 ggcgaccatc aggccgacct gacgcggatc gccgagtccc gcgaagagat cctggaagac
     1921 gccggtcgcc tcacgatgcc agtcccacga gaagtggtcg cggacgaact ggaggaccgg
     1981 cgtgatgtca tccggccggg caggacggat gtcgatgccg tattgctcga gcttctggta
     2041 ggcgggcgcg agggccggcg gaacggtgaa acccgtgagg atggcccgca tgctgagcgg
     2101 tcgcatcgtc acccggaagc catgcttctc gaagaagccg atcgccggtg cgtaggcgtt
     2161 cacgtcgacc ccgggtgtga aatagttcgg cacgtatggg ccgagcatga tcttcgcccg
     2221 tcccagttca cgcagctgat tcaggacgcc atgcagcagg gcacggccga tgccctgccg
     2281 ctgaaagtcg gggtcgacgc cgaacgcggt gatccagccc gttgtcggat ccagtccttc
     2341 gttgaagaac ggcacctggc gggtgagggc aagaacgaat ccccgcacga cgccgtcgac
     2401 gtcagcgacg aggcaagtcg cgggatcgaa gttcgggtcg agcaggacct tcgcgcgcca
     2461 ggtctcgaag gtgatcggat cgctccacag cgcgcggttc cagacgggaa cgagacttgc
     2521 ctcgtctcct cgctggtacg gtctgatggt gactgccggt gatggca
//
LOCUS       DASTMD010000598         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2734115, whole genome shotgun sequence.
ACCESSION   DASTMD010000598 DASTMD010000000
VERSION     DASTMD010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2734115"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..386)
                     /locus_tag="VFQ54_08805"
     CDS             complement(156..386)
                     /locus_tag="VFQ54_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165132.1"
                     /translation="MDSVTIVVGILCAMMAAIGLVIARISKKDRGAGLLIVVVAVAIL
                     VFFHAAPDRPPLPGSGTPSPDVPFSFPIKTNP"
     gene            complement(392..640)
                     /locus_tag="VFQ54_08810"
     CDS             complement(392..640)
                     /locus_tag="VFQ54_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165133.1"
                     /translation="MSNHTNQILQEIAYSAIGLTMIVIGLAFAVRRKQYSFGSILIFT
                     GAFIIGFAFYVSQANNLVFDDDSPEMDPAAYGTPAFGN"
     gene            890..1924
                     /locus_tag="VFQ54_08815"
     CDS             890..1924
                     /locus_tag="VFQ54_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEU0165134.1"
                     /translation="MASISRPHPASHAIHLHRRLFIASAVSTFIASARGSQTHAQGTP
                     GASSSPAPEGWVSGTSIPVARSEFPATVLDGKVYVAGGFGAESTFSRFDPVTSEWVDL
                     ASLPEPRNHLGLTALDGAVYLAGGHSSETMATDTFWRYDVASDRWDEEPVLPQGPRGA
                     LGCTALDGKVYVVGGSSRDLGGPATNDAAAYDPATGAWTTLPSLPTAREHLSVGSADG
                     LLIAVGGRDGSNVDISMTSANEVFDPKTGAWEKRAALPVPRSGMGVAADGSHIAVIGG
                     EGERGIYTAANWYDPATDTWSALPDLPGGRHGVAAAIVDNRLYAIGGSTQAWNVQNSF
                     GVDWLRLERP"
BASE COUNT          424 a          728 c          801 g          389 t
ORIGIN      
        1 ggagcatgtc atcgccgggc gtgtcgcgcc aacggtggat gggtgaccac gtgggtcacc
       61 gggacgggct ttcatcaagg gatgcgggcc aggcgacttg ccatcgacgt tggccagaat
      121 ggcgacgacg gacttggaga cgagactcgg cgatcctagg ggttcgtctt gatggggaac
      181 gagaagggga cgtccggcga gggcgtcccg ctcccgggga gcggaggtcg atcgggtgcg
      241 gcgtgaaaga agaccaggat ggcgacggcc acgaccacga tcagcagtcc cgcgccgcga
      301 tccttcttcg agatcctggc gatgaccaga ccgatcgccg ccatcatcgc gcagaggatt
      361 ccgacgacga tcgtgaccga atccacggcg atcagttgcc gaaggcaggt gtgccgtagg
      421 cggccgggtc catctccggt gagtcgtcgt cgaagacgag attattcgcc tgcgagacgt
      481 aaaacgcaaa cccgataatg aacgcaccgg tgaagatcag aatcgagccg aacgagtact
      541 gctttcgtcg cacggcgaag gcgagcccga tgacgatcat ggtcaaaccg atggccgagt
      601 acgcgatttc ctgcaagatc tgatttgtgt gattactcat cctgccgtct tggtcctcat
      661 cctgggggtg ttggcgggtg cgtggtgatc acccgcggtc gtgcggacag catacccgag
      721 aactaccacc ccaccgcccg tagatcccgc cgggcgcggc atgcagtcac ggatcacgat
      781 ccgcgcatgg cccgtccggg tcaggtcgcg gcgccaacct tctctatagt gaggtggtga
      841 agagcgcgtt ccgccgtcaa ttgcaaccgc cggaaaggat cgtcacatca tggcttcgat
      901 ctcgcgacca cacccggctt cccacgcgat ccacctgcat cgccgccttt tcatcgcgtc
      961 cgcggtctcg acattcatcg cttcggcgcg tgggtcgcag acacatgccc agggaacgcc
     1021 aggagcatcg tcatcacctg cccccgaggg ctgggtttcc ggcaccagca taccggtggc
     1081 gcgatccgag tttccggcaa cggtcctcga cggcaaggtc tacgtcgccg gtggattcgg
     1141 agcggaatcg accttctcgc ggttcgatcc cgtcacgagc gaatgggtcg atctcgcatc
     1201 gctgcccgaa ccccgcaatc acctcggcct gaccgcgctc gatggcgcgg tctatctcgc
     1261 cgggggccat tccagcgaaa ccatggcgac ggatacgttc tggcgctacg acgtcgcatc
     1321 cgatcggtgg gacgaggaac cggtcctgcc gcagggaccg cgcggcgcgc tcggttgcac
     1381 ggcgctcgat gggaaggtct acgtggttgg cggctcatcg cgcgatctcg gcggcccggc
     1441 aacgaacgat gccgccgcct acgatcccgc aaccggcgcc tggacgaccc tgccatcgct
     1501 gccgaccgca cgcgagcatc tctcggtcgg cagcgcggac ggtctcctga tcgcggtcgg
     1561 tggccgcgac gggtccaatg tcgacatcag catgacgagc gcgaacgagg tcttcgatcc
     1621 gaaaaccgga gcctgggaga aacgtgccgc gttgccggtt ccgcgctcgg ggatgggcgt
     1681 cgcagcagac ggatcgcaca tcgcggtgat cggcggcgag ggggaacggg gcatctatac
     1741 cgccgcgaac tggtacgacc cggcgacgga tacgtggtcc gcgctgccgg acctccccgg
     1801 cgggcggcac ggcgtcgccg cggcgattgt cgacaacagg ctctacgcga tcggcggcag
     1861 cacccaggcg tggaatgtcc agaacagctt tggtgtcgac tggctccgcc tcgaacgtcc
     1921 atagaggcgt tcccctcatt gagggaccag aagaccaaat gtcgggacga gggcgggatc
     1981 gttgaaggcg acgatccgcg aaatggaccc gatcggcggc ggggagcgat gccgtagggg
     2041 cgagtctgtg ctcgcccggt gcgccagagg gacgcgcggt gggaagcacg tggtgccgac
     2101 gcggtccctc gacaagctgc tctgaaaaac tccgcccttc gggccctccg accgaagctt
     2161 tttcatgccg cgctggcgag gcgcgcttca tgactactcg ggaggacgta tcttgtagga
     2221 agtcgtgcgt gaagcatgtg gataccggct gctccctcga caggctcggg agggcgtgtt
     2281 gcgtgcggcg tcgtgaggac ggccaaggcc caggggacgg agtaaccgga ggacgggtga
     2341 gg
//
LOCUS       DASTMD010000599         3095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1486121, whole genome shotgun sequence.
ACCESSION   DASTMD010000599 DASTMD010000000
VERSION     DASTMD010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486121"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1389)
                     /locus_tag="VFQ54_08820"
     CDS             complement(115..1389)
                     /locus_tag="VFQ54_08820"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165135.1"
                     /translation="MASRAALGPAPTGRSFNVMLAIIALSIGGFGIGTTEFAVMGLLP
                     DIADGMNVSIPHAGYAVSAYALGVVVGAPVLAFFGARWPRKHLLMTLMVVFALGNFLS
                     SVAPNFGLLIAARFFSGLPHGAFFGVAAVVAASLVEPSKRGQAVAKVMIGLAIANVFG
                     VPFATWVGQAFGWRLAFTIVAAIGLLAVGMIARFVPLQPVERRVGMSRELDILKRGQL
                     WLTLLIGTVGFGGVFAVYSYITPTMTKVAGFGDDAIPFILMAYGLGMVSGNILGGRMA
                     DKSVLGSIYFGMIAMTLVLFAFTFAAHNKVTAVIGVYLIGLMCSTIFPAVSMRLMDLA
                     PDGQSLASSMSHSAFNVANALGAWLGGLVISFGWGYTAPAAVGSVLAVLGIGVVWLST
                     RGDAARLAEDHQAQAHPVIEIAPPATATPGTD"
     gene            1691..2944
                     /locus_tag="VFQ54_08825"
     CDS             1691..2944
                     /locus_tag="VFQ54_08825"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0165136.1"
                     /translation="MGGPGSGEESRAGARPMPLSGIKVVDLTRVMTGPYAAMMLGDLG
                     ADVVKVEQPGKGDDTRLWGPPFIKGEASYFLTVNRNKRSIALDLKSAGGREVLWKLID
                     EADVLIENFSPGTMTRLGFGQEDVLARRPSLVYASISGFGQSGPSGNRTAYDLIVQGM
                     SGMMSVTGQDGGMPTKLGVPIADIAGGMFAAYAVMGALFARERDPERRGQYVDTSMLG
                     GQVALLTYQAGIWFTTGEVPTMLGNRHPIITPYDTFPTADGFINIAVGNDAIWERFCN
                     VLGLEEARDDPHFRTNADRSAHRAEVYAIVNAAFARHTTAELVDLLDTASVPCGPIQD
                     VQQVFDDPQVHHEDLILTLPHATLGEVTVAGRPYHFHGTPLEDRNGPPILGQQSEEIL
                     RELGYDDEAIAGLLACRAVEGPSAG"
BASE COUNT          578 a          978 c         1069 g          470 t
ORIGIN      
        1 aggcggcccg cggccgtggg ccaccgggag gatcgcgcgg caccctcgta gggtcagcct
       61 cgtgctgacc cggagccacg ttgcatgacc ggagtcctgg atggtcagtc gttgctagtc
      121 ggttcccggg gtcgccgtgg cgggtggggc gatctcgatc accgggtgcg cctgggcctg
      181 atggtcttcc gccagccgcg ccgcgtcgcc gcgcgtgctc agccagacga cgccgatgcc
      241 gaggacggcg aggaccgatc cgactgctgc cggcgcggtg tatccccatc cgaacgagat
      301 cacgagacca ccgagccagg cgccgagcgc gttcgcgaca ttgaacgccg agtggctcat
      361 cgatgatgcg agcgactgcc cgtccggcgc gagatccatc agccgcatcg agaccgccgg
      421 gaagatcgtc gagcacatca gcccgatcag gtagacgccg atcaccgccg tcaccttgtt
      481 gtgggcggcg aaggtaaagg cgaagaggac cagcgtcatc gcgatcatgc cgaagtagat
      541 gctgccgagc accgacttgt ccgccatccg gccgccgagg atgttgccgg agaccatgcc
      601 cagaccgtag gccatcagga tgaacgggat cgcgtcgtcg ccgaagcccg cgaccttcgt
      661 catcgtcggt gtgatgtagg agtagaccgc gaagacgccg ccgaacccga cggtaccgat
      721 cagcagcgtc agccagagct ggccgcgctt caggatatcg agttcgcggc tcatgccgac
      781 ccggcgctcg accggctgga gcgggacgaa gcgcgcgatc atcccgaccg cgagcaggcc
      841 gattgcggcg acgatcgtga aggcgaggcg ccagccgaaa gcctgtccga cccatgtcgc
      901 gaacgggacg ccgaagacgt tcgcgatcgc gaggccgatc atcaccttcg cgaccgcctg
      961 accgcgtttc gatggctcga cgagcgaggc ggcgacgacg gcggcaacgc cgaagaaggc
     1021 gccgtgggga agaccggaga agaaccgcgc cgcgatcagg agcccgaaat tcggagcgac
     1081 actggagagg aagttcccga gcgcgaagac gaccatcagc gtcatcagga ggtgcttgcg
     1141 aggccagcgc gcgccgaaga aggcgagcac cggtgccccg acgacgacgc cgagcgcgta
     1201 cgcggagacc gcgtagccgg cgtgtgggat cgagacgttc atgccgtccg cgatatccgg
     1261 aagcaagccc atcaccgcga attcggtcgt gccgatcccg aaccccccga tcgagagggc
     1321 gatgatggcg agcatgacat tgaaggaacg accggtcggg gccgggccga gtgcggcgcg
     1381 cgaggccaag ggatactcct ggagacacgt tgggaattga atggatcggg ggaggggcga
     1441 aggcgtggga aagagccgtt cgccgacaca cttacattct acaagggcag ccggattccg
     1501 tgtaaacgtc atgtgagtgc ggtggttggg ccacatgtgc gtccggaccg agcgatcacg
     1561 ggcaggttca ggcatcccgc ccggaaagct gacccctgtc gaacgttcac gcggtattat
     1621 ccctgcacgt tgtcaaagaa acgttgagtt cgagatcgac cgagcctgaa ggcgtgacag
     1681 gggcatcatc gtgggtggac cgggtagcgg tgaggaatcc agagcgggtg cgcggccgat
     1741 gccgctgagc gggatcaagg tggtggacct gacccgggtc atgaccggtc cctacgccgc
     1801 gatgatgctc ggcgatctgg gggcggatgt cgtcaaggtc gagcagccag gcaagggcga
     1861 cgacacgcgc ctctgggggc caccgttcat caagggagag gcgtcctatt tcctgaccgt
     1921 caatcgcaac aagcgctcga tcgccctcga tctcaagtcg gcgggcgggc gggaagtcct
     1981 ctggaaactg atcgacgagg cggacgtgtt gatcgagaac ttctcgccgg gcacgatgac
     2041 ccggctcggc ttcgggcagg aggatgtcct cgcgcggcgg ccgtcgctcg tctacgcctc
     2101 gatctccggg tttgggcagt caggaccgtc agggaaccgc accgcctacg acctcatcgt
     2161 gcaggggatg agcggcatga tgagcgtcac cggacaggac ggcgggatgc caaccaagct
     2221 cggcgtgccg atcgcggaca ttgccggcgg catgttcgcc gcctacgccg tgatgggagc
     2281 cctcttcgcc cgcgaacgcg atcccgaacg gcgcggccag tacgtcgaca cctcgatgct
     2341 gggtgggcag gtcgcgctgc tcacgtacca ggccggcatc tggttcacca ccggcgaagt
     2401 gccgacgatg ctcggcaatc gccatccgat catcacgccg tacgacacct tcccgacggc
     2461 agacggcttc atcaacatcg ccgttggcaa cgatgcgatc tgggagcgct tctgcaacgt
     2521 gcttgggctc gaggaagcgc gcgacgatcc gcatttccgg acgaacgcgg atcgctccgc
     2581 gcaccgggcg gaggtctacg cgatcgtcaa cgcggcgttc gcccggcata cgaccgcgga
     2641 gctcgtcgat ctgctcgaca cggcgtcggt cccgtgtggc ccgatccagg acgtgcagca
     2701 ggtcttcgac gatccccagg tccatcacga ggacctgatc ctgacgttgc ctcatgcgac
     2761 gttgggcgag gtgaccgtcg ccggtcgtcc ctaccacttc cacggaacgc cgctggagga
     2821 ccggaacggt ccgccgattc tcggacagca gagcgaggag atcctgcgtg agctcggcta
     2881 cgacgacgag gcgatcgctg gcctccttgc ctgccgtgcc gtcgagggac catccgcggg
     2941 atgacgggtg ccgggatcat cgctctcaga aggcagcttg ttctcgatcg actcggtcga
     3001 ggatttcccg ccagtcgtcc cgacgcggcg tgacgtcgaa ctcggtgatc agcatccgca
     3061 cgaccgacgc gagcgtcaca aagccggtcg ggatg
//
LOCUS       DASTMD010000600         1664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1486123, whole genome shotgun sequence.
ACCESSION   DASTMD010000600 DASTMD010000000
VERSION     DASTMD010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486123"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFQ54_08830"
     CDS             complement(<1..231)
                     /locus_tag="VFQ54_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165137.1"
                     /translation="MSAESQDIKDMDIAVEPGDDRAYELARMRHSAAHLMAEAVQELF
                     PDARFGIGPAIDNGFYYDFDLPRALTPDDLVEI"
     gene            complement(326..>1664)
                     /gene="thrS"
                     /locus_tag="VFQ54_08835"
     CDS             complement(326..>1664)
                     /gene="thrS"
                     /locus_tag="VFQ54_08835"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU0165138.1"
                     /translation="AYWRGDEKRPMLQRIYATAWFTREELDDYLHKLEEAARRDHRKL
                     GRELDLFSVSEEIGPGLILWHPKGGMIRFLIEQFEQTEQLARGYDLVYTPHIASEKIY
                     KTSGHLETYGENMYAPMQIEEVDYYLKPMNCPGHIMIYKNSVHSYRELPIRLAELGTV
                     YRYERSGTLHGMLRVRGFTQDDSHIFCTHDQVIDEVGGVIDLAIHMAEIFGYRFQAYL
                     GTRPAKAIGSDDVWEEATNNLKQALASRGLDYILDEGEGAFYGPKIDIKWLDALGRAW
                     TGPTIQVDFNLPERFDVNYIAEDGERHRVAMIHRTLLGSMERFVGGLVEHYAGAFPAW
                     LAPVQAVIIPITDDQIEFANKVEARLKDAGLRANVDLSSGRMQAKIRNAQLQKIPYML
                     VIGGREAEADSVAVRLRSGEDLGAKTIQEFIDLVKPVIATKSLDLQAARTTGS"
BASE COUNT          283 a          487 c          568 g          326 t
ORIGIN      
        1 gatctcgacg aggtcgtcgg gggtgagggc gcgggggaga tcgaagtcgt agtagaagcc
       61 gttgtcgatg gcgggaccga tgccgaatct ggcgtcgggg aagagctcct ggacggcttc
      121 ggccatgagg tgggcggcgg agtgacgcat gcgagcgagc tcgtaggcgc gatcgtcgcc
      181 gggttcgacg gcgatatcca tgtctttgat gtcttgtgat tcggcgctca tgtttgacgt
      241 ctcccggatg ttcagcagtc tcctgaaaac gaaacggccc ggcgttcctt gcggagcacc
      301 gagccgggaa gagggcaagg ctccgctagg agccggtagt tcgtgccgcc tggagatcga
      361 gcgacttcgt ggcgatcacc ggcttcacga gatcgatgaa ctcctggatc gtcttcgcac
      421 cgaggtcctc accggagcgg aggcgaacgg cgaccgaatc ggcctccgcc tcgcgtccac
      481 cgatgacgag catgtacggg atcttctgaa gctgggcatt gcggatcttc gcctgcatgc
      541 gcccgctgga gaggtcgacg ttggcgcgga gtccggcgtc cttgaggcga gcctcgacct
      601 tgttcgcgaa ctcgatctgg tcgtccgtga tcgggatgat cacggcctgg accggcgcaa
      661 gccaggccgg gaaggcgccg gcatagtgct cgacgagacc gccgacgaaa cgctccatcg
      721 agccgagcaa cgtgcggtga atcatggcga cgcggtgtcg ctcgccgtcc tcggcgatgt
      781 agttcacgtc gaagcgctcg gggaggttga agtcgacctg gatcgtcgga ccggtccagg
      841 cgcgaccgag ggcatcgagc cacttgatgt cgatcttcgg gccgtagaag gcgccctcgc
      901 cctcgtcgag gatgtaatcg agaccgcgcg acgccagcgc ctgcttgagg ttgttcgtcg
      961 cctcttccca gacgtcgtcg ctgccgatcg ccttcgccgg tcgcgtgccg aggtaggcct
     1021 ggaagcgata gccgaagatc tcggccatgt ggatggcgag gtcgatcaca ccgccgactt
     1081 cgtcgatcac ctggtcgtgc gtgcagaaga tgtgcgagtc gtcctgcgtg aagccacgga
     1141 cgcggagcat gccgtgcagg gtgccggatc gctcgtagcg gtagacggtg ccaagctctg
     1201 cgagccggat tggcagttcg cgatacgagt gcaccgagtt cttgtagatc atgatgtgac
     1261 cggggcagtt catcggcttg aggtagtagt ccacctcctc gatctgcatc ggtgcataca
     1321 tgttctcgcc gtacgtctcg agatgccccg atgtcttgta gatcttctcg gaggcgatat
     1381 gcggcgtgta gacgagatcg tagccgcggg ccagctgctc cgtctgttcg aactgttcga
     1441 tcaggaagcg gatcatgccg cccttcggat gccagaggat cagacccggt ccgatctcct
     1501 cggagaccga gaagaggtcg agctcacggc cgagcttgcg atgatcgcgc cgggcggctt
     1561 cctccagctt gtgcaggtag tcgtcgagct cttcccgcgt gaaccacgcc gtcgcgtaga
     1621 tccgctgcag catcggtcgc ttctcatcgc cacgccagta ggca
//
LOCUS       DASTMD010000601         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2466196, whole genome shotgun sequence.
ACCESSION   DASTMD010000601 DASTMD010000000
VERSION     DASTMD010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2466196"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFQ54_08840"
     CDS             complement(<1..198)
                     /locus_tag="VFQ54_08840"
                     /inference="COORDINATES: protein motif:HMM:NF015996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEU0165139.1"
                     /translation="MEGTLVPRIANLITLALIIVGGLNWFLVGVFKFDLVAAIGGGHD
                     FGETNGFIRTIYVIVGLAAIVQ"
     gene            complement(231..806)
                     /locus_tag="VFQ54_08845"
     CDS             complement(231..806)
                     /locus_tag="VFQ54_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165140.1"
                     /translation="MSDPKSTSETIVDWVGDIVAVESHIEEALDHQLRLTPGSRDVAE
                     MITQFHDTVRDSKKRAVAYQETIGTTSGNGVIKKGSELLGKAAGMIDRIRKDSPTKSL
                     RDDYVAFNLAAISYSLLQTTALALDDQKTQAFAEEGLATYAKLVLKLNAIVARAVVDD
                     LITNSEVQVADPDVVSESSATIARIWQEAAN"
     gene            complement(1048..1890)
                     /locus_tag="VFQ54_08850"
     CDS             complement(1048..1890)
                     /locus_tag="VFQ54_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HEU0165141.1"
                     /translation="MTAIPAAAKQVTLNQVVDANTLQVTSSGVKGEIHLFSDDAVSPV
                     TCGTQQAADFARWALSFNDTPGTVYVQQDSKVRDVDFVWFKSGGQPYLLNEALVRNGW
                     SQPGTTTGSYATQLKNAASLAKTHLLGNWSACGGFDIALSQRVPQASVNVPLPPSSQP
                     ASTQPGATDTSAPQQDTSGGSIPDNSGSDTTGTSDYPDTSSDQGDTSQPDTAAPQEDT
                     GTYVGDDGCTYTNVSGQQVSCPVQADSPPAGATAQCNDGTYSFSQHRQGTCSGHGGVA
                     QWLQ"
     gene            2428..2652
                     /locus_tag="VFQ54_08855"
     CDS             2428..2652
                     /locus_tag="VFQ54_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165142.1"
                     /translation="MREALQPVQICRDIRDLILKAILQLANLTFQIDDPGDQRIHRLL
                     NGAVDASVDPRYQIAQVRFVVEMLHARLLA"
     gene            complement(2687..3205)
                     /locus_tag="VFQ54_08860"
     CDS             complement(2687..3205)
                     /locus_tag="VFQ54_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165143.1"
                     /translation="MANVDLAAEHRLLRFLMDPSWSPDKPWGDAISFADAADATSLSE
                     SHVKDLVLRFADRGWISLTSSRAGWNDGTTFFARMTDEGMREVEGRPIKVQVDGPVEV
                     AIAAIGGLVLTADDAERLRALLLAVDATPDERSKVSLLTQALGIASNATTSAEGLRAF
                     LDGMTEIIHSIT"
BASE COUNT          652 a          960 c          935 g          673 t
ORIGIN      
        1 ctgcacaatg gcggccaggc cgacaatgac atagatcgtt cggatgaatc cgttcgtctc
       61 gccgaaatcg tgccctcctc caatggcagc cacaaggtcg aacttgaaca ccccgacgag
      121 gaaccagttc aatccaccga cgatgatgag tgcgagcgtg atcaggttag caatacgtgg
      181 cacgagcgtt ccttccatgc gcggcgagcg aattcgctct gatgcagacg ttagttggcg
      241 gcttcctgcc aaatccgggc aatcgtcgcg ctgctttcgg agacaacgtc cgggtccgct
      301 acctggacct cgctattggt gatcaagtcg tccactactg ctcgagccac gatggcgttg
      361 agtttgagca ccagctttgc gtatgttgcg agcccttcct cggcgaacgc ctgcgtcttc
      421 tggtcatcaa gggccagcgc tgtcgtctgc aggagggagt agctgatggc ggcaaggttg
      481 aacgccacat aatcgtcacg caaggacttc gttggcgaat cttttcgaat gcgatcgatc
      541 atgcccgccg ccttgccgag cagctcggac cccttcttga tcacaccatt ccccgaggtc
      601 gtaccaatag tttcttgata cgcgactgct cgcttcttgc tgtcgcggac cgtatcgtga
      661 aactgcgtga tcatctcggc cacgtcgcga ctgcccggcg taagacgcag ttggtgatcg
      721 agggcttctt cgatgtgact ttcgacggcg acaatatccc ccacccagtc gacgatagtt
      781 tcagaggtgg atttcggatc agacacagca cataccctta tggttaggca tttcagaacg
      841 ttgaaacgcc ctcgtccttt tcgatggcac cattgccatg gagcctgcga tgcacatcct
      901 gtgccagacg ttatgacaaa gggcgccaaa gggtatgctg catctcatcg gtatggcaga
      961 tactcgacaa gactccggca ccaacattat ttcggggatg aagatcggcc ggggagcaac
     1021 ctccggccga tcttcatgtc agctaggcta ctgaagccac tgggcgacgc cgccgtgccc
     1081 cgagcaggtg ccctggcgat gctggctgaa cgaataggtc ccgtcgttgc actgcgccgt
     1141 agcgccagcc ggtggcgaat ccgcctgcac tgggcagctc acctgctgtc ccgagacgtt
     1201 ggtatacgtg catccgtcat caccgacgta cgtgccagtg tcctcttgcg gggctgccgt
     1261 gtccggctgc gacgtatcgc cctgatcgct gcttgtgtcg gggtaatcgg atgttcctgt
     1321 cgtgtctgat ccggagttgt ccgggatcga gccgccagag gtgtcctgtt gcggagcaga
     1381 tgtgtctgtg gctcccggct gagtggacgc cggttgtgag ctcggtggca gtggcacatt
     1441 gacggacgcc tgcgggactc gctggctcag tgcgatgtcg aacccgccgc acgcggacca
     1501 gttcccaagc agatgggtct tggctaagga tgctgcattc ttaagttgcg tcgcatacga
     1561 tccggtcgtc gtacccggtt gagaccaccc gttgcgaacc aatgcttcat tgagcaggta
     1621 gggctgaccg cccgatttga accagacgaa atcgacgtcg cggaccttgc tgtcctgctg
     1681 cacgtagacc gtgcccggcg tgtcgttgaa actcaacgcc caccgagcga agtccgccgc
     1741 ctgctgcgtt ccacacgtaa caggcgaaac tgcgtcatca gagaagaggt ggatttcccc
     1801 cttgacacca ctcgatgtga cctgcagcgt gttcgcgtca acgacctgat ttagtgtcac
     1861 ttgcttcgcg gccgcaggga tagcggtcac ccagatcgcc ggagcgaagc cgccatgcgg
     1921 cagctctgga caagcgatcc catccccatc ggtatcgagt gacgcatacg taatcttgtt
     1981 ggactcatac gtagtctgtg cccactccca cgcatcgaag gttgcacagg tagttggcgg
     2041 cggtgccgca ccagcaggcg tcgcggagag ggatgaactt atcgcaaaca gcgaaaggca
     2101 tagcgtaaag acgagcgcga gaagacggcg catgtgttcc cccaaagcaa ggatggcaag
     2161 tatgacgatg gtgacccgtg ttgctccggt cggaataata cttgtggggt tacgaaggcg
     2221 gtatccgtaa aactacgtat ttatcgggat gcatctaagt acacgtatgg cgctggccat
     2281 gctcaaaaag ggatcagacg tgacagatcg cacacctgcc tgtctctcga tcccagacga
     2341 aaggggcgtg atcaagcttg atcacgcccc tttcattcag ttgcttgaaa actatggcaa
     2401 gccgctgaat gaggatatcc gggctgtgtg cgagaagcgt tacagcccgt ccagatctgt
     2461 cgcgatatcc gggacctcat cctgaaggcg atcctgcagc tcgcgaacct gactttccag
     2521 atcgacgatc cgggcgacca gcggatccac cgcctcctca atggcgcggt cgatgcgagc
     2581 gttgaccctc gatatcagat cgcgcaggtt cgtttcgtcg ttgaaatgct gcacgccaga
     2641 ctcctcgcct aactgggatc agtccaacta tcggaagatc ccacactcac gtgatcgaat
     2701 ggatgatctc ggtcatgccg tccagaaagg ctcggagccc ttccgcgctt gtggtcgcgt
     2761 tcgaggcgat accgagcgcc tgtgtgagca acgatacctt cgacctctcg tcgggggtcg
     2821 cgtcgacggc aagaaggagc gcgcgaagcc gttcggcatc atcagcggtc aggaccaatc
     2881 cgccgatcgc cgcgatcgcg acttctaccg gaccgtcgac ctggaccttg atgggacgcc
     2941 cctccacctc ccgcatgccc tcgtcggtca tccgggcgaa gaacgtcgtc ccgtcgttcc
     3001 accccgcgcg cgaggacgtc aaactgatcc agccgcgatc cgcgaaacgc aagacgagat
     3061 ccttgacgtg cgactccgaa agcgaggttg cgtcagcggc atcggcgaag ctgatggcat
     3121 cgccccaagg tttgtccggg gaccaggacg ggtccatcag gaagcgcaag agtcgatgtt
     3181 cggccgccaa atccacattt gccattcgat ttgatccctc
//
LOCUS       DASTMD010000602         1828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_323233, whole genome shotgun sequence.
ACCESSION   DASTMD010000602 DASTMD010000000
VERSION     DASTMD010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323233"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFQ54_08865"
     CDS             complement(<1..232)
                     /locus_tag="VFQ54_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165144.1"
                     /translation="MTERSPANKERRSGSTAAAARRNGVRPQRDAEVPTYVVEHQAES
                     RTLGGLFSGTETQDLPAVVRLSGVATRDRRLQG"
     gene            complement(404..952)
                     /locus_tag="VFQ54_08870"
     CDS             complement(404..952)
                     /locus_tag="VFQ54_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165145.1"
                     /translation="MPDWLHTDDLKFDRIPLGPFDTRLWWLLIGVCVVLVFTIQAIET
                     AIEGAWPNQRRPGRMLPRARWVQASWAFVALFVLAGALLTIGNVALLIWKNPPHPDEL
                     GLGSWLLAIGWVLFLLFSLNALGLGRLMGNLGLIGPLAIVMILVVGDILLLIAFQHLF
                     PSVKDVRDGIENGLKEILPFWD"
     gene            complement(1312..>1828)
                     /locus_tag="VFQ54_08875"
     CDS             complement(1312..>1828)
                     /locus_tag="VFQ54_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU0165146.1"
                     /translation="DDRVVYLGTFSKTIAPGIRTGWTVAPPDIHRMLLAAREIIDISN
                     DRVTQRTVFQTAAGDFDTRIARARDIYRARRDMMLASLNEYMPEGVTWSQPQGGFFVW
                     VTLPESFDVNEFVPKAATHGTVVFPGDWFFQGRDVRNKIRLSFSTVPEHRIQEGIRRL
                     SAAIMDEFATH"
BASE COUNT          359 a          548 c          613 g          308 t
ORIGIN      
        1 ggccctggag acggcggtcg cgcgttgcca cgccggagag gcggacgaca gccggaaggt
       61 cctgggtttc ggttccgctg aagagcccgc ccagcgttcg tgactccgcc tggtgttcga
      121 cgacatacgt cgggacttca gcgtcgcgtt gcggacgcac tccgttgcga cgagcggcgg
      181 ctgccgttga tcccgaccgg cgttccttgt ttgcgggcga tctctctgtc atctgctgga
      241 gcgcctttcc cgcctcattc gtacgcgtac ggtcgcgccg atgttacagg atacgttgcc
      301 cacccatgtc acggttgggt gccctgtcat cgtcacgacg ggggcgaagt gatgccctcg
      361 tcgatcgtac acgagaacgt caatatgcgc gtgtgacaaa ttgctaatcc cagaacggaa
      421 gaatctcctt gaggccgttc tcgatcccgt cacgcacgtc cttgaccgag ggaaagagat
      481 gttgaaaggc gatcaggagc aggatatcgc cgacgacgag aatcatgacg atcgccagcg
      541 gtccgatcag gccgagattc cccatcaggc gcccgagtcc gagcgcgttg agactgaaga
      601 ggaggaagag cacccacccg atcgcgagca accagctgcc gagtccaagc tcgtccggat
      661 gcggcggatt cttccagatc agcaaggcga cgttgccgat cgtcagcagg gcgccggcga
      721 ggacgaagag cgccacgaat gcccaggagg cctgcaccca gcgcgcgcgg gggagcatcc
      781 ggccagggcg acgttgattc ggccaggctc cttcgatcgc agtttcgatc gcctggatcg
      841 tgaagacgag aacgacgcac acaccaatca gaagccacca gagacgggta tcaaaggggc
      901 cgagcgggat acggtcgaat ttgaggtcgt cggtgtgcaa ccagtccggc atcttttggg
      961 atatcgcccg ggattctgca tcgcgcttct tgggtccgat gagcggccgc gaatccgatg
     1021 cgaggaggtg ccgagaggag acgggtgagc gtgacgatcg gcaagtctct tgtcgcagga
     1081 cacggcacgc gggctatgac gccaggagga tgatgacaga atcggtcggg acgggatcgc
     1141 gcggaggcga tcgacgtcct agcaaatact tcgtgcgcgg gcgcgatgag acgcgtccgg
     1201 ggcacagggt ccgtagcagc agcgccggca ccatgggaga gcgtagcaca gacggcgaac
     1261 ggggtcggat tccacccgga accccgaccc cgttcgcgaa aaatcaggca actagtgcgt
     1321 ggcaaactca tccatgatcg ccgccgagag gcgacgaatg ccttcctgga tccgatgctc
     1381 gggaaccgtc gagaaactga ggcggatctt gttccggacg tctcgtccct ggaagaacca
     1441 gtcacccggg aagacgacgg tgccgtgggt cgccgccttc ggcacgaact cgttgacgtc
     1501 gaaggattcc ggcaacgtca cccagacgaa gaatccgccc tgtggctgcg accaggtcac
     1561 gccttccggc atgtactcgt tcagggaggc cagcatcatg tcccgccgcg cccggtagat
     1621 gtcgcgggcc cgggcgatgc gggtgtcgaa atcaccggcg gccgtctgga agaccgtccg
     1681 ctgcgtaacg cgatcgttgc tgatgtcgat gatctcgcgc gcggcgagga gcattcggtg
     1741 gatgtccggc ggcgcgaccg tccagcccgt gcggatgccc ggcgcgatcg tcttcgagaa
     1801 ggtgccgaga tagacgacac gatcgtcc
//
LOCUS       DASTMD010000603         1537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_680122, whole genome shotgun sequence.
ACCESSION   DASTMD010000603 DASTMD010000000
VERSION     DASTMD010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680122"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFQ54_08880"
     CDS             complement(<1..201)
                     /locus_tag="VFQ54_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165147.1"
                     /translation="MNHHLFARATGKLLVAVTMVVSSLSGAFALSASAAAAASSGPID
                     LPAAVPTVAQLDKAGLTGFVPNG"
     gene            complement(315..848)
                     /locus_tag="VFQ54_08885"
     CDS             complement(315..848)
                     /locus_tag="VFQ54_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0165148.1"
                     /translation="MNDRQTPSSPPSEDPPTDDLSVTASHLNSGAIHLLRGLRIVDRE
                     AGLTPARLSALSVLVFGGPRSLGRLAEAEDVAGPTMTRIVDGLVDLGLAQRVAHPENG
                     RMTTILATDAGMTLMHDAAQKRIDAIVAALGTLSDQDRGRIQEAAPALRRLAMILRND
                     ILTPGAHISEQAGTIDS"
     gene            907..1296
                     /locus_tag="VFQ54_08890"
     CDS             907..1296
                     /locus_tag="VFQ54_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0165149.1"
                     /translation="MDLLQVAQRATDLDRAVAFYTRLLGAPPVARFDPPGLAFFMLGP
                     VRLLVDRAAVDRTAPGSLIYLKVDDVRTRIGELRTDGVTIEGEPHIIFSHADDTLGPT
                     GTDEWHGFIRDSEGNLVGLVSHLPKES"
BASE COUNT          286 a          510 c          492 g          249 t
ORIGIN      
        1 gccgtttggc acgaagccgg tgagccccgc cttgtcgagc tgcgcgaccg tcggcacggc
       61 ggccggcaaa tcgatgggac cggacgacgc ggcagccgca gccgacgccg acagggcgaa
      121 tgccccactc aatgacgaaa caaccatggt caccgcgacg aggagcttcc cggtcgcacg
      181 cgcaaaaagg tgatggttca tggacagaca ccctcatccg gaagcgtgat agacgacgat
      241 cgatacaacc tgcacagtgt ttgaacgcca gagtgagccg gtcagttccg cccgataccg
      301 acagcattca gacgtcacga atcgatcgta ccggcttgtt cggagatatg cgcaccgggg
      361 gtaaggatgt cattccgcag aatcatggca aggcggcgca aggccggggc cgcctcctga
      421 atccgcccac gatcctgatc cgaaagggtt cccagcgccg cgacgatcgc atcgatccgc
      481 ttctgggccg cgtcgtgcat cagcgtcata ccggcatccg tcgccaggat cgtcgtcatc
      541 cgtccgttct cgggatgcgc gacccgctgc gcgagcccga ggtcgacgag gccatcgacg
      601 atccgcgtca tcgtcggacc ggcgacatcc tccgcctccg cgagccgccc gagcgaccgc
      661 ggcccgccga agacgagcac cgagagcgcg ctgagccgcg ccggcgtcaa tcccgcctcg
      721 cgatcgacga tgcgcagacc gcgcagcagg tggattgccc ccgagttgag atgactcgcg
      781 gtgacggaga ggtcatccgt tggagggtct tcggaaggcg ggcttgaagg agtctggcga
      841 tcgttcatct ccacatgata gcatcactac acattaatag gcatgctatg aaaggaacga
      901 cgcctcatgg atctcctgca agtcgcccag cgtgcgaccg atctcgatcg cgcggtcgcc
      961 ttctacacca ggttgctcgg cgcgccgccg gtcgcccggt tcgacccacc cgggcttgcc
     1021 ttcttcatgc tcggaccggt tcgcctgctc gtcgatcgtg cggctgtcga tcgtacagcc
     1081 ccgggctccc tgatctacct caaggtcgac gacgtccgga cgcggatcgg ggaactgcgc
     1141 accgacggcg tgacgatcga gggcgaaccg cacatcatct tcagccatgc ggacgacacg
     1201 ctcggtccga ccgggacgga cgagtggcac ggcttcatcc gcgattcgga aggcaacctg
     1261 gtcggcctcg tcagtcatct gccaaaggaa tcctgaccgc cacccgtgaa tgaggcgatt
     1321 ggggagcgcg ccgtgagatc ggatgcgccc tcattcccgt cgccgcggcc cttgtggccg
     1381 cttcgggtcg gctatgccgg cccggatcgg gggaacgccg gggtgcgggg tcgctcaccc
     1441 ctggctcagg acgacggcga ggcgttgtac ggaaagcacg cggtaccaaa ccggtccctc
     1501 gacaagctcg ggaagacgtg ttctcgatat cggcgga
//
LOCUS       DASTMD010000604         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1752435, whole genome shotgun sequence.
ACCESSION   DASTMD010000604 DASTMD010000000
VERSION     DASTMD010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752435"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VFQ54_08895"
     CDS             <1..719
                     /locus_tag="VFQ54_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165150.1"
                     /translation="FGDLRVQLLKPFHNSSYVLGTDANGRDILTRLAYGGRVSMTVAG
                     LSLLFTLVIGGAVGSIAGFFGGLIDSILMRLVDVVISIPGITLLLILSVLWKPGPTGL
                     ALVIAAISWTGISRLIRGEVLTLKHRDYIDAARIIGATDRRIIMNHIFPNVVSIIIVW
                     ASLALPSLIITEATLSYLGFGVQIPTPSWGNMLNEASQFYTQSWTYAFLPGFMIFLAA
                     LSFNLVGNGLRDALDPRLNN"
     gene            919..1386
                     /locus_tag="VFQ54_08900"
     CDS             919..1386
                     /locus_tag="VFQ54_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEU0165151.1"
                     /translation="MSENVIRCPNCGAENQVGDHFCSNCGTRLLRPRPAPPAEPETPQ
                     EEPVVEEEVVPEPIAPPPPVQSFPPPIATTFSQQNPPPPADEDEGSWRMSSLGPPPRS
                     RRRLWLWIVIGLIAACLIVCVAAAIFLNTNTGQNWLDDVGTSVAKEATKQAGN"
     gene            complement(1462..>1695)
                     /locus_tag="VFQ54_08905"
     CDS             complement(1462..>1695)
                     /locus_tag="VFQ54_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165152.1"
                     /translation="VRVDRVEDVLNIGDEVEVLVIEVAPNGKVSLSRRAALTGVLPDP
                     KPARPRDDRGPRRDDRRGGGNNGGGGGGFRPRS"
BASE COUNT          305 a          599 c          482 g          309 t
ORIGIN      
        1 ccttcggcga tctccgcgtc cagctgctga agccattcca caacagcagc tacgttctcg
       61 gaaccgacgc caacggtcgc gacatcctga cccgtctcgc ctatggcggt cgcgtctcga
      121 tgacggtcgc cggtctctcc ctgctcttca cgctcgtgat cggtggcgcc gtcggctcga
      181 tcgccggctt cttcggcggc ctgatcgaca gcatcctgat gcgcctggtc gacgtcgtga
      241 tctcgatccc gggcatcacg cttctgctga tcctctccgt gctctggaag ccgggaccaa
      301 ccggactcgc gctcgtcatc gcggcgatca gctggaccgg catctcgcga ctgatccgcg
      361 gtgaagtgct gaccctcaag catcgcgact acatcgacgc cgcccgcatc atcggcgcga
      421 ccgatcgtcg catcatcatg aaccacatct tccccaacgt cgtctcgatc atcatcgtct
      481 gggcatcact cgccctgccg tcgctgatca tcacggaagc gaccctgagc taccttggct
      541 tcggcgtcca gatcccgacg ccttcctggg gcaacatgct caacgaagcg agccagttct
      601 acacccagtc gtggacctac gcgttccttc cgggcttcat gatcttcctc gccgcgctct
      661 ccttcaatct cgtcggcaac ggtttgcgcg acgccttgga tccgcgcctc aacaactagg
      721 ctcgattcaa tcgaacacaa cggcacgtga cggagcgggc gacccggtat gggtcgcccg
      781 ctccgcgttc ccgggaccgg gacgtaacga cgcagtagct aacgaccggc acctatcggc
      841 attggcccgg aatccgcgta cgtttacact caaggcagca cagaatgttc gatcgatggg
      901 actaacgacg ggagttggat gagcgagaac gtgattcgat gtccgaactg cggggccgaa
      961 aaccaggtgg gcgatcattt ctgctcgaac tgtggaacgc gcctgctccg accccgaccc
     1021 gctcctccgg cggagccaga gactccccag gaagaaccgg tcgtcgagga agaagtggtt
     1081 ccggaaccga tcgctccccc accacccgta cagtcgttcc cgccaccaat cgcaacgacc
     1141 ttcagccagc agaatcctcc tcccccagct gatgaggacg agggcagctg gagaatgtcg
     1201 agtctcgggc cgccgccgcg gtcacgccgc cgcctctggc tctggatcgt catcggcctg
     1261 atcgccgcct gcctcatcgt ctgcgtggcg gcagccatct tcctcaacac caacaccggc
     1321 cagaactggc tcgacgacgt tggaacgtcg gttgccaagg aagccacgaa acaagccgga
     1381 aactgatcgc accacgcctc acgcgaaagc cccgagtcat tgctgactcg gggctttctc
     1441 tatgtctggc tgaatgttgt tctacgagcg agggcggaag ccgccgcccc caccgccgtt
     1501 attgccgcca ccacggcgat cgtcgcggcg cgggccgcga tcgtcccgcg ggcgagccgg
     1561 cttcgggtcc gggaggacgc cggtaagcgc cgcgcgacgg gagaggctga ccttcccgtt
     1621 cggtgcgacc tcgatcacca ggacctcgac ctcgtcgccg atgttgagaa cgtcctcgac
     1681 gcgatcgacg cgaac
//
LOCUS       DASTMD010000605         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1216439, whole genome shotgun sequence.
ACCESSION   DASTMD010000605 DASTMD010000000
VERSION     DASTMD010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216439"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFQ54_08910"
     CDS             <1..659
                     /locus_tag="VFQ54_08910"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA methyl transferase PRC-barrel
                     domain-containing protein"
                     /protein_id="HEU0165153.1"
                     /translation="DQSYVLYTLDQDQLGFIQFPLGKLTKPEVRQLARAYGLPVADKA
                     ESQEICFVGNRSYADFVSERRPDVTVAGEIVTAEGELLGQHRGLVHHTVGQRRGIGIA
                     APKPYFVLHLDTGSNRLVVGSREEAEASQLTIEDVTLSTGAWPTEPFLADAVVRYRGM
                     PTPARIVPGPAGSRTASLEFVGDRAPIASPGQAVVFYLGDQVLGGGTIQQVARRGRAA
                     "
     gene            808..1218
                     /locus_tag="VFQ54_08915"
     CDS             808..1218
                     /locus_tag="VFQ54_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165154.1"
                     /translation="MGGVKSKALWAGIGTLLVFVLIIVGLYLLGGSDQSALERLTDIA
                     IIFVVLLSMITVVLLAAVTVALVFLVLQIKDNVIPLLEEATGTVKRIRGTTNFVTEEA
                     VKPIVTVAGTYSRIRGMTKVVVGKSKRPPKLPKS"
     gene            1525..1965
                     /locus_tag="VFQ54_08920"
     CDS             1525..1965
                     /locus_tag="VFQ54_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165155.1"
                     /translation="MVDAELRMLRNTAHSARKRLDRLDRDLSRFAHRAPRIVDPRSIR
                     RRETSAATRGFMIGAAVSGAVALFLSRARTVLAPARSTVARLTSGVGGGWTVAHSSLD
                     RPVRSEADAAYLNLAEASIDSGAMAHTDVAAHTVEDGGVESRDA"
     gene            complement(1984..2394)
                     /locus_tag="VFQ54_08925"
     CDS             complement(1984..2394)
                     /locus_tag="VFQ54_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165156.1"
                     /translation="MASIDFIYINQQAVRLTSFTRDQQSSRLSLIVIVRGNAARDELN
                     ALVAREPITVAFPDEEPAEMHLASVDLKTTGEGARSIHRFDLTLVAWANQANSDISPD
                     ATTDSAAESALMRRLDAIEQKLDQLTALVRDRLH"
     gene            2557..3762
                     /locus_tag="VFQ54_08930"
     CDS             2557..3762
                     /locus_tag="VFQ54_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165157.1"
                     /translation="MIVFPIITAVISAMCAGVLAYDAKRRPRPDKIVWAIAFLAFTVA
                     AASDAAGRAIEWTTWLARTYYSTGPALVVMYLAIGELYLLVPKQMARFGVGATLLLTA
                     FWIAMVYSAPIDHARLADDGWEAIKRGTVMTLITVLINSIGTTIIVGGTGYSVWNFWR
                     KGIMRNRMIGCALIALGTIAVGAGGTLTRLGHYEYLYIAMSIGVAMIFAGVLQTRRPD
                     VVVAAGVAAAPATASATVAAPAPVVAHTSASSLNGAVPSGDTPASVDVPSIAATGDPI
                     AYIEGVLLPLRDHELARTCAEWSVPRDDSAVLTRSEARHVWRLRSHLSAEGMRGFDAH
                     TVMARRQLTVLWDEVLTANVDAPIADAAPVSQALPPPAESRPEMARLASMQRAPDMTD
                     EDEIASTYA"
     gene            4020..>4539
                     /locus_tag="VFQ54_08935"
     CDS             4020..>4539
                     /locus_tag="VFQ54_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU0165158.1"
                     /translation="MTEINPAILLLLVAFAAPLVVSAVGIRRPDLVPNTSIVMTGLAF
                     IAVLWSFTRDDATFDAVWASTWDIRFTVTLDGLAREYALLATGIGLAVVVFAAWYLPL
                     HLHHHHRSERELPRFFGFLLLFMAAMVGLVMAQDLILLFVFWDLTAIASFYLIGYDRD
                     EAESRSSAMMALL"
BASE COUNT          736 a         1426 c         1499 g          878 t
ORIGIN      
        1 aggatcagag ctacgtgctc tatacgctcg accaggacca gcttggcttc atccagtttc
       61 cgctcgggaa gttgacgaag ccggaggtgc gacaactggc acgagcatac gggctgccgg
      121 tggcggacaa ggccgagagt caggagatct gcttcgtcgg caatcgctcg tatgcggatt
      181 tcgtcagcga gcgccggccg gacgtcaccg tcgccggcga gatcgtgacg gccgaaggcg
      241 agctcctcgg ccagcaccgg ggtctcgttc atcacacggt cggacagcga cgcgggatcg
      301 gcatcgcggc gcccaagccc tacttcgtcc tccatcttga caccggcagc aaccgtctgg
      361 tggtgggatc gcgcgaggaa gcggaggcga gccagctgac gatcgaggat gtcacgttga
      421 gcactggcgc ctggccgacc gagccattcc tggcggacgc cgtcgtgcgt taccgcggga
      481 tgccgacccc ggcgcggatc gttcccggtc cggccgggag ccggacggcg tcgctggaat
      541 tcgtcggcga ccgcgcgccg atcgcctcgc cagggcaggc cgtggtcttc tatctcggcg
      601 atcaggtgct cggcggtggc acgatccagc aggtcgcgcg acgcggtcgc gcggcctgag
      661 atcgtgcgcg cgatcgcggc gcgcatcgtg cgctagggta gtgagtctcg cccacagagt
      721 gagattcatg cacaatagcg tctacattcg ggcgatgttc cgcgttgccg ctgactgaac
      781 gagcaaaaca ggagacggag atactgcatg ggtggcgtca agtcgaaagc actctgggcg
      841 ggaattggca cgctcttggt gttcgtgctg attatcgtag gactctatct cctgggaggc
      901 tccgatcaat cggcgctgga gcgactgacg gacatcgcga tcatcttcgt cgtcctgctc
      961 tcgatgatca ccgtcgttct cctggcggca gtcacggttg cgctcgtctt cctcgtgctc
     1021 cagatcaagg acaacgtgat tccgctgctg gaggaggcga ccggtaccgt gaagcgcatc
     1081 cgtggcacga ccaacttcgt gaccgaagaa gcggtgaagc cgatcgtgac ggtcgctggt
     1141 acctactcgc ggatccgtgg gatgacgaag gtcgtcgtcg gcaagtcgaa gcgaccgccg
     1201 aagctgccca agagctagtc gaaacgcatc agagggtctg gaagaacgct gccggcgatg
     1261 tcgttggcag cgtttctgcg cgcatacggc gaaaccgaaa tgggtgccag gcatcctgca
     1321 ggatcggaaa tggtgtcctg acgatccgat acgatgaggg cccggggagt ggctgtcccg
     1381 ccgctgccgg accgcagtcc ggacggcctg tcccgccgtg ctcgcggtgg cagcgtttct
     1441 gcgtgcccgg acaacggatg tcaccaacca acgcgtgacg ttgaccccac cgacgcgttt
     1501 ccgtcagacc aggagggtcc caagatggtt gatgccgaac ttcgtatgct tcgaaatacc
     1561 gcgcattcgg cgcgaaaacg actcgaccgc ctggatcgcg atctctcgcg tttcgcgcac
     1621 cgtgctcctc gcatcgtcga tccccggtcg atcaggcggc gtgagaccag tgccgcgacg
     1681 cgcggtttca tgatcggcgc tgccgtaagc ggcgccgtcg cgctcttcct ttcccgggcg
     1741 cgcaccgtcc tcgcaccggc gcggtccacc gtggcccggc tcacgagcgg ggtcggcggt
     1801 ggatggaccg tggctcactc ctcactcgat cgtcccgttc ggagtgaagc ggatgccgcc
     1861 tatctcaacc tggcggaggc ttcgatcgat agcggcgcga tggcccacac cgatgttgcc
     1921 gcgcacaccg tcgaggacgg cggggtcgag agccgcgacg cctgatcgcg cccggcgacg
     1981 cgttcagtgc aggcgatcgc ggacaagagc ggtgagttgg tcgagcttct gttcgatcgc
     2041 gtcgagacgt cgcatcaatg cgctctcggc ggctgaatcg gtggtcgcgt ccggcgagat
     2101 gtcgctgttc gcctgattcg cccatgccac gagtgtgaga tcgaaccggt ggatcgagcg
     2161 agcgccttcg ccggtcgtct tgaggtcgac tgatgcgagg tgcatctcgg caggttcctc
     2221 gtccgggaag gcgacggtga tcggttcgcg ggcgacgagc gcgttcaatt cgtcgcgcgc
     2281 cgcattcccg cgcacgatga cgatcagcga gaggcgcgat gactgctggt cccgcgtaaa
     2341 ggacgtgagg cgaaccgcct gctggttgat gtagatgaaa tcgatcgagg ccatgattct
     2401 tcccgatgga tgacggcgga aggcgacttt cggagcggct gcacgagatg cccgctccgg
     2461 tcggatgagc atgcgccaga agagtacaat ccgaccagag caaatgagtg aatgcccacg
     2521 attttggcgt ggtacctcgg tgcaggtgtc tccgacgtga tagtttttcc gattattacc
     2581 gccgtgatca gcgcaatgtg tgcgggtgtc ctcgcgtacg acgcgaagcg gcgtccccgt
     2641 ccggacaaga ttgtctgggc gatcgccttc ctcgcgttca cggtcgcggc cgcgtccgat
     2701 gccgcgggac gcgcgatcga gtggacgacg tggctcgccc gaacttacta ctcgactggc
     2761 ccggcgctgg tcgtgatgta cctcgcgatc ggcgagctct acctcctcgt gccgaaacag
     2821 atggcgagat tcggtgtcgg tgcgacgttg ctgctcaccg cgttctggat cgcgatggtc
     2881 tacagcgcgc cgatcgatca cgcgcggctc gcggacgatg gctgggaggc gatcaagcgc
     2941 ggcaccgtga tgacgctgat cacggtgctc atcaactcga tcgggacgac gatcatcgtc
     3001 ggtggcaccg gatattcggt ctggaacttc tggcgcaagg ggatcatgcg caaccggatg
     3061 atcggctgcg cgctgatcgc cctcggcacg atcgccgttg gcgccggtgg tacgctgacg
     3121 cgtctcggtc actacgaata tctctacatc gcgatgtcga tcggggtggc aatgatcttt
     3181 gcgggcgtgc tgcagacgcg gcgtcccgat gtcgtagtgg ctgcgggagt ggccgctgcg
     3241 cccgccaccg cctcggccac ggtagccgcc ccggccccgg tcgtcgccca cacctcggca
     3301 tcgtccctca acggggccgt accttcgggc gacacgccgg cgagcgtcga cgtcccctcc
     3361 atcgcggcaa cgggagatcc gatcgcctac atcgagggag ttctcctgcc gctgcgggat
     3421 catgaactcg cccggacctg cgcggaatgg agcgttccgc gggacgactc ggcagtcctc
     3481 acgcgctccg aggcgcgcca cgtctggcga cttcgttccc acctctccgc ggaggggatg
     3541 cggggtttcg acgcccatac cgttatggcc cgccgtcagc tgacggtcct ctgggacgaa
     3601 gtcctcaccg ccaacgtcga cgccccgatt gcggacgccg cgccggtgtc ccaggcattg
     3661 ccaccacccg cggaatctcg tcccgagatg gcccggctgg catccatgca gcgagcaccg
     3721 gacatgacgg atgaggacga gatcgcctct acctacgcgt gagtcccggt gccggtccgg
     3781 tcgggcgtgg tacagttaca aaaagtaaca agcgttgctg actgagccgt ccccggacgc
     3841 gctggtcgcg cgcaggttaa tcttcgatcg attgcgatcg gccccacatt ccccgaagat
     3901 cattcgtgta tcgcacgcta caaatgtggc ggccctggcg gttttgcgag aagctggctt
     3961 ttgccggccg cagtctcgtt ttgtccctgt ttttctgttc gtcaacgaga ggagcgacga
     4021 tgacggagat caatccagcc atcttgttgc tgctcgttgc cttcgcggcg ccgctcgtgg
     4081 tttccgcggt ggggattcgc cgacccgatc ttgtcccgaa tacttcgatc gtgatgacgg
     4141 ggctcgcgtt catcgcggtg ctctggtcgt tcacgcgcga cgacgcgacg ttcgacgccg
     4201 tctgggcctc gacgtgggac attcgattca ccgtcaccct cgacggcctg gctcgcgaat
     4261 acgcactgct ggcgacgggt atcgggctcg cggtcgtcgt cttcgcggcg tggtaccttc
     4321 cgctgcatct ccaccatcac caccgctccg aacgcgagct gccgcgcttc ttcgggtttc
     4381 tgctgctctt catggcggcg atggtcggcc tcgtgatggc gcaggatctg atcctgctct
     4441 tcgtcttctg ggatctgacc gcgattgcct cgttctacct gatcggctat gaccgcgatg
     4501 aggcggagtc gcgcagctcg gcaatgatgg cgctgcttg
//
LOCUS       DASTMD010000606         3897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_769581, whole genome shotgun sequence.
ACCESSION   DASTMD010000606 DASTMD010000000
VERSION     DASTMD010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769581"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFQ54_08940"
     CDS             complement(<1..569)
                     /locus_tag="VFQ54_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU0165159.1"
                     /translation="MTYDFINPDFGLALPQLAVFITAIVLALGDAFLPKRLQFTWLTA
                     ISLLGYAIGLVLLFTQHDKNESTFYGLFRADGLTVFLSLIILSAGVLTVLISASYVEN
                     LEGRMPIGEFYTLLAFAILGSLLTASAGDLVMIFVGIELSSLATYILTAFAKRRETSL
                     EGALKYFLLGIFASAILLYGMAWIYGATGS"
     gene            complement(630..2279)
                     /locus_tag="VFQ54_08945"
     CDS             complement(630..2279)
                     /locus_tag="VFQ54_08945"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU0165160.1"
                     /translation="MDSFPILSCIAYAPAVCALLILLVPQANKELPRRLAVLGTGLSF
                     LLSLIPLFGLKHNADFQYTEHKLWLKDLGISYFLGVDGISVLLVAITSLLGLLAVIWS
                     WSTIDHRPREYYIGLLLVVTGMLGIFMALDLFIFYIFWEVMLIPLALLIGVWGGPNRV
                     YAAVKFFLYTFFGSLLMLVGIVATYQAYFNQTNIRTLNVLELQGGNYSHDFQFWVFAA
                     FFIAFAIKVPVFPFHTWLPDVQMQAPTAASVMLTSEIGAYGIIRFCLPLFPQGLKDWT
                     PVIITLSLIAVVYAALIAFVQPDVKRLVAYASISHLGLLTVGLFVGNIQGMDGAIMLM
                     MAHGFNTGAMFLMAGMLYQRAKSQEIDSFGGLATRMPIWTTFFAIFVLASIGLPGLSS
                     FVGEFLVSLGTWHYNRWLTIVTFGVVILSAWYMLTFFQRMVFGRASRELPDPGDGELT
                     DGERRELAAAGGGHGLGHGGLAPVSGGAHEPGDADVAIATHEGQLDSIDWTDLTPKEI
                     LTLLPLAALTIFFGVYPKPILTIAEPSFQAILSGAARVVGN"
     gene            complement(2372..>3897)
                     /gene="nuoL"
                     /locus_tag="VFQ54_08950"
     CDS             complement(2372..>3897)
                     /gene="nuoL"
                     /locus_tag="VFQ54_08950"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU0165161.1"
                     /translation="RRRSAVNAARKAFIVNRIGDVGFGIGIVYTFWTFKTINFYGPDG
                     IFADAANRTALGIAGGTLTAIGILLFIGAMGKSAQFPLHVWLPDAMEGPTPVSALIHA
                     ATMVTAGIYMVARTHVIYMQSETAMLVVALIGAFTAFMAATIAITQNDIKRVVAYSTL
                     SQLGYMAIALGTGAWVPAIFHLMTHAFFKALLFLGAGSVIHGMHEEQDIRKMGGLRKY
                     MPITFWTFLVGALANAGVIPFAGFWSKDEIISSAWVSDSSVGKLVAIVGLATAFLTAV
                     YMFRLFFLVFMTKPRFDEQEVHPHESGRVMTWPLIILAVPTILIGFVGFPPDKGAIHD
                     FLEPVFYSAPAQATEPAPGTTAAPATAAVAEQPEPHEVSTTTQWVFGIISTIVALGGI
                     WVAWLAYIRRSLDVAALTRRFSGLYDFLLNKWYWDEIYDLVIVKPLAAFARFIGTVVE
                     IGIIDASVNGVAYGIGAISQRLRRVQTGLVANYAFAIALGMILLVGIYIAGFSNLLR"
BASE COUNT          805 a         1158 c         1337 g          597 t
ORIGIN      
        1 gagccggtcg cgccatagat ccaggccatg ccgtagagca ggatcgcgct cgcgaagatg
       61 cccagcagga agtatttcag cgcaccctcg agtgacgtct cgcgccgctt cgcaaaggcg
      121 gtgaggatgt aggtcgcgag cgaggagagc tcgatcccga cgaagatcat cacgagatcg
      181 cccgccgatg ccgtcagcaa cgaaccgagg atcgcgaatg ccagcagggt gtagaactcg
      241 ccgatcggca tccggccctc gaggttctcg acgtagctgg cggagatgag gacggtgagg
      301 acgccggcac tgaggatgat cagcgagagg aagaccgtca ggccgtcggc ccggaagagc
      361 ccgtagaacg tgctctcgtt cttgtcgtgc tgcgtgaaga gcaggacgag tccgatcgca
      421 tagccgagca gcgagatcgc ggtcagccag gtgaactgaa gccgcttcgg cagaaaggcg
      481 tctcccagcg cgagcacgat cgccgtgatg aagacggcga gttgcgggag cgccagcccg
      541 aagtccgggt tgataaagtc gtaggtcatc ggctcggctc caccatactg cgatcgggag
      601 aagtgaacgg gtctttcaag gcggcgctcc tagtttccga cgacgcgcgc ggcgccggag
      661 aggatcgcct ggaaactcgg ctcggcgatg gtcaggatcg gcttcgggta gacaccgaag
      721 aagatcgtca gcgccgccag cggcagcagg gtcaggattt cctttggcgt gagatcggtc
      781 cagtcgatgc tgtcgagctg gccctcatgg gtcgcgatcg cgacatccgc gtcgcccggt
      841 tcgtgggcgc caccgctgac cggggcgaga ccgccgtgcc caagcccgtg cccaccaccg
      901 gcagctgcca gttcgcgccg ctcgccatcg gtcaactcac cgtcgccggg atcgggaagc
      961 tcgcgggagg cgcgtccgaa gaccatccgc tggaagaacg tcagcatgta ccacgcggag
     1021 aggatgacga caccaaaggt gacgatcgtc agccagcggt tgtaatgcca ggtgccgagc
     1081 gagacgagga attcaccgac gaagctcgag aggccgggaa gaccgatgct ggcgagcacg
     1141 aagatcgcga agaaggtcgt ccagatcggc atccgcgtcg cgaggccgcc gaaggaatcg
     1201 atctcctggc tcttcgcgcg ctggtagagc atgcccgcca tcaggaacat ggcgccggtg
     1261 ttgaaaccgt gcgccatcat cagcatgatc gcgccatcca tcccctggat attgccgacg
     1321 aagagaccga cggtgagcag gccgaggtgg ctgatcgagg cgtaggcgac gagccgcttc
     1381 acatccggct gcacgaaggc gatcagcgcc gcgtagacca ccgcgatcag cgagagcgtg
     1441 atgatgaccg gcgtccagtc cttcagcccc tgcgggaaga gcgggaggca gaagcggatg
     1501 atgccgtagg cgccgatctc ggaggtcagc atcaccgacg ccgcggtcgg cgcctgcatc
     1561 tggacatccg gcagccaggt gtggaacggg aagaccggca ccttgatcgc gaaggcgatg
     1621 aagaaggcgg cgaagaccca gaactggaag tcgtgactgt agttgccgcc ctggagctcg
     1681 aggacgttca gcgtccggat gttcgtctgg ttgaagtacg cctggtaggt cgcgacgatg
     1741 ccgacgagca tcagcaacga gccgaagaag gtgtagagga agaatttcac cgccgcgtag
     1801 acgcggttcg gaccgcccca gacgccgatc agcagcgcca gcgggatcag catcacctcc
     1861 cagaagatgt agaagatgaa gagatcgagc gccatgaaga tgccgagcat gccggtcacg
     1921 accagcagca ggccgatgta gtactcgcgc ggtcgatggt cgatcgtgct ccacgaccag
     1981 atgacggcaa gcagaccgag cagcgacgtg atcgccacga ggaggacgga gatgccgtcg
     2041 acgccgagga agtagctgat cccgagatcc ttcagccaca gcttgtgctc ggtgtactgg
     2101 aagtccgcgt tgtgcttcaa gccgaagagc gggatcagcg agagcaggaa gctcagaccc
     2161 gtgccgagca cggccagccg tcgtggcagc tccttgttcg cctgtgggac gagcaggatc
     2221 aggagcgcac acaccgccgg ggcgtatgct atgcagctga gaatcggaaa gctatccaca
     2281 agctcctccg gagcggttca cggatcatcg agatccagga cgacgtcgtt gtctggcggc
     2341 ttccggtccg atccgtaggc cgcgcatcct cctagcgcag gaggttcgag aatccggcga
     2401 tgtagatccc gacaagcagg atcatcccca gcgcgatagc gaaggcgtag ttggcgacga
     2461 gacctgtctg cacgcgacgc aatcgctggc tgatcgcgcc gatcccatag gcgacgccgt
     2521 tcaccgaggc atcgatgatc ccgatctcga ccaccgttcc gatgaagcga gcgaacgcgg
     2581 caagcggctt gacgatgacg aggtcgtaga tctcatccca gtaccacttg ttgagcagga
     2641 agtcgtagag accggagaag cgtcgcgtca gcgccgcgac atcgagcgag cgacggatgt
     2701 aggcaagcca ggcgacccag atgccgccga gggcgacgat cgtcgagatg atgccgaaga
     2761 cccattgcgt cgtcgtcgag acctcgtgcg gctcgggctg ctcggcgacg gcggcggttg
     2821 ccggcgcggc ggtcgttccc ggcgcgggtt ccgtagcctg ggccggcgcc gagtagaaga
     2881 ccggctccag gaaatcatgg atcgctccct tgtccggcgg gaagcccacg aagccgatca
     2941 ggatcgtcgg gacggcgaga atgatcagcg gccacgtcat gacgcgcccc gattcgtgcg
     3001 gatggacctc ttgctcatcg aagcgcggct tggtcatgaa gacgaggaag aagagccgga
     3061 acatgtagac ggcggtcagg aacgccgttg ccaagccaac gatcgcgacg agcttgccga
     3121 ccgaggaatc gctgacccag gcgctggaga tgatctcgtc cttcgaccag aagccggcga
     3181 acgggatcac cccggcgttc gccagcgcgc cgacgaggaa ggtccagaag gtgatcggca
     3241 tgtacttcct gaggccgccc atcttccgga tgtcctgctc ttcatgcatc ccgtggatga
     3301 ctgagccggc gccgaggaag aggagcgcct tgaagaaggc atgcgtcatc aggtggaaga
     3361 tcgccgggac ccaggcgccg gtgccgagcg cgatcgccat gtagccgagc tggctcaggg
     3421 tcgagtaggc gacgacgcgc ttgatgtcgt tctgggtgat cgcgatcgtc gccgccatga
     3481 aggcggtgaa ggccccgatc agcgcgacga ccagcatcgc ggtctcggac tgcatgtaga
     3541 tgacgtgggt ccgcgccacc atgtagatgc cagcggtgac catcgtcgcc gcgtggatga
     3601 gcgcggagac cggcgtcggg ccttccatcg cgtccggcag ccagacgtga agcgggaact
     3661 gggcgctctt gcccatcgcg ccgatgaaga gcaggatccc gatcgcggtc agcgtcccgc
     3721 cggcgatgcc gagcgcggtg cgattcgcgg cgtcggcgaa gattccgtcc gggccgtaga
     3781 agttgatcgt cttgaaggtc cagaaggtgt agacgatacc gatcccgaag ccgacgtcac
     3841 cgatgcggtt gacgatgaag gccttcctcg cggcgttgac cgcggagcgg cgacgga
//
LOCUS       DASTMD010000607         5077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1664953, whole genome shotgun sequence.
ACCESSION   DASTMD010000607 DASTMD010000000
VERSION     DASTMD010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664953"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFQ54_08955"
     CDS             complement(<1..224)
                     /locus_tag="VFQ54_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165162.1"
                     /translation="MSREDGHVVDLMDDTRLNVASLLMEPVGNTRDVELHLTALPLDD
                     DLVARDIETVARLTRLRGQILVSGTIQGHV"
     gene            552..1010
                     /locus_tag="VFQ54_08960"
     CDS             552..1010
                     /locus_tag="VFQ54_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0165163.1"
                     /translation="MNEMELDSGLESEVGAVAAALLLSINLLRRQLRQSPMAGEPTLP
                     EFAALSRLDRGGPATSSALAKLEQISPQSMGATLAALETRGLVERRDDPEDGRRVVLS
                     ITEAGVAMLQEKRTARAEQISRALASGFTREELEQLKTAAPLIERLAQNI"
     gene            1105..2811
                     /locus_tag="VFQ54_08965"
     CDS             1105..2811
                     /locus_tag="VFQ54_08965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165164.1"
                     /translation="MALSNTTLAVFMSALDGSIVIISLPAIFRGIHLDPLASSNVSYL
                     LWMLMGYMLVQAILVVTLGRLGDMFGRVRIYNLGFVVFTVASVLLSFDPFDGGHGALW
                     LIGWRLLQAFGGSMLTANSAAILTDAFPTEQRGFALGINQIAALAGQFIGLVAGGLLA
                     VWDWRSVFWVNVPVGVFGTIWAYRMLRDSGDRHPGRIDWWGNITFAVGLGAVLIAITN
                     GINPYGGHSMGWTNPKVYGSMIGGVILLAIFVWIELKVDEPMFQPSLFKIQAFTAGNL
                     AGLAASIARGGLQFMLIIWLQGVWLPLHGYNYEDTPLWAGIFLLPLYAGFLVAGPASG
                     FLSDRFGARGFATAGMVVFGASFVGLMLLPIDFSYWAFAVLIALNGVGLGMFSSPNTS
                     AIMGAVPARQRGVASGMRATFQNAGTSLSIGIFFSLMIAGLASSLPDTLTNGLQQQGV
                     PSDVAHQIGSLPPVSSLFAAFLGVNPVENLLTPNGVLASLPAANQQVLIGKEFFPNLI
                     SGPLHHGLFVVFSAATILAALAAIASLLRGKQVVPPTIPLRPHSDSLQIAPRQQPIEG
                     GE"
     gene            3129..4910
                     /locus_tag="VFQ54_08970"
     CDS             3129..4910
                     /locus_tag="VFQ54_08970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165165.1"
                     /translation="MRGGSGTRDDGAAGDSYKWVALANTTAAVFMSALDGSIVIISLP
                     AIFRGIHLDPLATGSVSYLLWMIMGYRLVQAVLVVTVGRLGDIFGRVRIYNSGFLVFT
                     IASVLLSFDPFDGGHGALWLIGWRILQALGGSMLTANSAAILTDAFPSEQRGFALGIN
                     QIASIAGQFVGLTAGGLLAVWDWRAVFWVNVPVGIFGTLWAYLKLRETAVRRDGPVRI
                     DWWGNLTFAVGLSAILIAITNGIHPYRNHSQGWLNPTVYGTIIAGLVLLVAFVFIENR
                     VEEPMFQLRLFRIQAFAAGNLAGLASSIARGGLQFMLIIWLQGIWLPLHGYNYDDTPL
                     WAGIFTLPLSSGFLVAGPISGFLSDRFGARGLATGGMVLFGASFVGLLLLPVNFSYWM
                     FAILITANGIGTGMFASPNSSSIMGSVPAAQRGAASGMRATFQNAGTSLSIGVFFSLM
                     IAGLASTLPTTLTDGLQQQGVPADVAHQIGTLPPVSTLFAAFLGVNPVQNLLEPTGVL
                     SSLPDQNREVLIGKEFFPHLISGPFHHGLLVVFSVATILSILAALASLLRGEGPDRSQ
                     SASRRARPQEHDSLQMAANQRPAQGGK"
BASE COUNT          841 a         1590 c         1575 g         1071 t
ORIGIN      
        1 tggacgtgac cttgaatcgt acccgagacg agaatctgcc cccgcaaccg cgtgaggcgg
       61 gcaaccgtct cgatgtcacg ggcgaccaga tcatcatcga gcggaagcgc cgtcagatga
      121 agctcgacat cccgcgtatt cccgaccggc tccatcagca gcgaagcaac gttgagccgg
      181 gtgtcgtcca tcaggtcgac gacgtgacca tcttcacgag acatggcgtt ttctttatcg
      241 cgtcttctgt gctgccagtg gatcgtaccg aagtggtcgc cgggaggcat gcacgaaaac
      301 gccggggata aaacccggcg taactgtgtc agtataggcg agccgtgcgt caagtttcaa
      361 tccatccccg gcccggcggg catcgcgccc agcggggagg ctctggcgag cgtcccgtgg
      421 gcgaatttcg ttcaggatgg acgcgatatc tcgccgtgca tattgataag ttaaaactca
      481 tcagtctaga cttagtaaca atgcgcgtgg gaaagcaacg gggcacccac atccaggaag
      541 gcggaaggtt gatgaacgag atggagctcg acagtggttt ggaatcagag gtcggcgcgg
      601 tagccgcggc acttctcctg agtatcaatc tgcttcgccg gcagttgcgt caatcgccaa
      661 tggcaggtga gccaacgttg ccagagttcg ccgcgctctc gcgcctggat cgtggtggcc
      721 ccgcaacatc gagtgcgctg gcgaagctcg aacagatcag cccgcagtcg atgggggcaa
      781 ccctcgccgc cctcgaaacc cgaggtcttg tcgagcgccg cgatgacccg gaggatggca
      841 ggcgggtcgt tctctccatt accgaggcgg gcgtcgcgat gctccaggaa aagcggactg
      901 cccgggccga gcagatctcg cgagccctgg cgagtggatt cacgcgcgag gaactcgagc
      961 agttgaagac ggcggcgccg cttatcgaac gtctggcgca gaacatctag ggcccactat
     1021 cgggccagat caaaaggcta tcccatgtcc agtgctttag cggcggagcc gggaaatcgc
     1081 tccgggaacg attcgtacaa atgggtggcc ttgtcgaata cgacgctcgc cgtgttcatg
     1141 tccgcgctcg acggatcgat cgtcatcatc tcgttgccgg cgatctttcg cggtatccac
     1201 ctcgatccgc tcgcttcctc gaacgtcagc tacctgttgt ggatgctgat gggctacatg
     1261 ctcgtccagg cgattctcgt cgtcacgctc ggtcgtctcg gcgacatgtt cgggcgggtt
     1321 cgcatctaca atctcggctt cgtcgtcttt accgtcgcgt cggttctctt atccttcgat
     1381 cccttcgacg gcggacacgg ggcgctgtgg ctgattggct ggaggctgct ccaggcattc
     1441 ggtggctcga tgctcaccgc aaactcggcg gcgatcctga ccgacgcatt tcccaccgag
     1501 caacgtggat tcgcgttggg aatcaatcag attgccgcac tggcgggaca gttcattggt
     1561 ctggtcgcgg gtggcctgct cgcggtctgg gattggcgct cggtcttctg ggtcaacgtc
     1621 cccgtcggcg tcttcgggac gatctgggcc tatcgcatgc ttcgggatag cggcgatcgc
     1681 catcccggcc gtatcgactg gtggggcaac atcacgttcg ccgtcggact gggcgccgtc
     1741 ctgatcgcga tcaccaacgg catcaatccc tacggcggac actcgatggg gtggacgaac
     1801 ccgaaggtat acggctcgat gatcggcggc gtcatcctgc tcgcgatctt cgtctggatc
     1861 gagctcaagg tcgacgagcc gatgttccag ccgagtctct tcaagatcca ggcgttcacg
     1921 gcgggaaatc tggcgggtct ggccgcgtcc atcgcccgcg gtggcttgca gttcatgctg
     1981 atcatctggt tgcagggcgt ctggctgccg cttcacggct acaactatga ggatacgccg
     2041 ctctgggccg gaatcttcct gctaccactc tacgccggat tcctcgtcgc tggtcccgcg
     2101 tcgggattcc tctcggacag attcggcgcg cggggtttcg ccaccgccgg gatggtcgtc
     2161 ttcggtgcga gcttcgttgg tttgatgctc ctcccgatcg acttctcgta ctgggcgttt
     2221 gccgtgctga tcgccctgaa cggggtcgga ctcggcatgt tcagctcccc gaacacgtcg
     2281 gcgatcatgg gtgcggttcc cgcgcgacag cggggcgtgg cgtccggaat gcgggcgacg
     2341 ttccagaatg ccggcacgtc cctctcgatc ggcatcttct tctcgctgat gatcgccggg
     2401 ctggcgagca gcctcccgga caccctgacg aacggcctgc aacagcaggg cgtgccgtcc
     2461 gacgtcgcac atcagatcgg atcgttgcca cccgtctcgt cgctctttgc ggccttcctc
     2521 ggggtgaacc ccgtcgagaa cctgttgacg ccgaacggcg tgctcgcttc gctgccggcc
     2581 gcgaaccagc aggttctgat cggcaaggaa ttctttccca acctgatctc gggaccgctt
     2641 caccacgggc ttttcgtggt cttcagcgcc gcgacgatcc tggcggcact cgccgccatc
     2701 gcgtcactcc tgcgcgggaa gcaggtggtt ccgccaacga ttccgttgcg accgcattcc
     2761 gattcgctgc agatcgcgcc gaggcaacaa ccgatcgagg gaggggagta ggcgagagcc
     2821 tgccgacttc taccgaaacc ccaacgaggc gaaacgggcg tgcgtatttc ggaggaggcg
     2881 ggcagtgtca aactgcccgc ctcccccgga ccggatattt gttttctttc attgctaagc
     2941 tcagactgat aagtgtggac ttagttcctt ggtcgagcgt tctgctggaa cccggtactg
     3001 gaggctggca tggacgcgaa tctcaagcag ccggatgttc cgatgtcgtc aggctggcga
     3061 tcgggtgaac ggcggcaagc gtctggtgtt ctgatgtcag gagtgacgtc cgaacgggga
     3121 ggactcgggt gaggggtgga tcagggacgc gcgatgacgg cgccgcgggg gactcctaca
     3181 agtgggtagc cctcgcgaac acgaccgccg ccgtcttcat gtcggcactc gacggatcga
     3241 tcgtgatcat ctcgctgccg gcgatctttc gcggcatcca tctcgatccg ctcgccacgg
     3301 ggagcgtcag ttatctcctc tggatgatca tgggttaccg gctcgtccag gcggtcctgg
     3361 tcgtgaccgt cggtcgtctc ggcgatatct tcggacgggt ccggatctac aactccgggt
     3421 ttctggtctt cacgatcgcc tcggttctcc tatccttcga tcccttcgac gggggacatg
     3481 gggcgctgtg gctgatcgga tggcggatcc tgcaggcgct cggtggctcg atgcttaccg
     3541 cgaactccgc ggcgatcctg accgacgcgt ttccctccga acagcgcggc ttcgcgctgg
     3601 gaatcaatca gatcgcgtcg atcgccggcc agttcgtcgg gctcacggcc ggcggtctgc
     3661 tcgcggtctg ggattggcga gcggtcttct gggtcaacgt tccggtcggg attttcggga
     3721 cgctctgggc ctatctcaag ctccgggaga cggcggtgcg acgcgacgga ccggtgcgga
     3781 tcgactggtg gggcaatctg acctttgccg tcggtctgag cgcgattctg atcgcgatca
     3841 cgaacggaat ccatccttat cgcaatcact cgcaaggctg gctcaatccc acggtatacg
     3901 gaacgatcat cgctggcctc gtgcttctcg tcgccttcgt ctttatcgaa aaccgggtcg
     3961 aggagccgat gttccagctg cggctctttc ggatccaggc gttcgcggcg ggcaacctgg
     4021 ctggtctcgc gtcgtcgatc gcccggggtg gcctgcagtt catgctgatc atctggctcc
     4081 agggaatctg gctgccgctg catggctaca actacgacga tacgccgctc tgggccggga
     4141 tcttcaccct gccgttgtcc tccggcttcc tggtggcggg tccgatctcc ggattccttt
     4201 cggatcgatt cggcgcacgc ggcctcgcca ccggtgggat ggtactcttc ggcgccagct
     4261 tcgtcggtct ccttctcctc cccgtgaact tttcctactg gatgttcgcg atcctgatca
     4321 cggcgaacgg gatcggcacc ggaatgtttg cctcgccgaa ctcgtcctcg atcatgggga
     4381 gcgttcccgc cgcccaacgg ggagcagcgt cgggaatgcg cgctaccttc cagaacgcag
     4441 ggacatcgct ttcgatcggt gtcttcttct cgctgatgat cgcgggactc gcgagcacgc
     4501 tccccacaac cctgaccgat gggctccagc agcagggcgt cccggccgac gttgcccatc
     4561 agattggcac gctgccaccc gtctctaccc tgtttgctgc cttcctcggc gtcaatccag
     4621 tgcagaacct gctggaaccg accggcgtgc tgtcatcgct accagaccag aatcgagagg
     4681 tgctcatagg caaggagttc ttcccgcatc tcatttccgg gccattccac cacggtctgc
     4741 tcgtggtctt cagtgtggcc accatcctct cgatcctcgc cgcgctcgcc tcgttgctgc
     4801 gtggcgaagg accagaccgg tcgcagtccg cgtcaagacg ggcgcgaccc caggaacacg
     4861 attcgttgca gatggcagcg aaccaacgcc cagcccaagg aggcaagtag tgaccgtaac
     4921 cacgatcgac ccgaagcccg ccctcgtcgt gatcgacctt cagaagggga ttctcgcgac
     4981 gcccaccatc catcccatgg atgaagtcgt cgccaatgcc acgaagctcg ccgcgtcgtt
     5041 tcgtgatcgg ggatttcccg tcgtgctcgt taatgtc
//
LOCUS       DASTMD010000608         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_680895, whole genome shotgun sequence.
ACCESSION   DASTMD010000608 DASTMD010000000
VERSION     DASTMD010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680895"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1029)
                     /locus_tag="VFQ54_08975"
     CDS             complement(67..1029)
                     /locus_tag="VFQ54_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165166.1"
                     /translation="MIEDQLRSDFERARQKAFLHDLVATATGRQNDLIPFHEVRKRLN
                     PEGESYRGLQTVPIDKIVGSMDRFQDFDRTFLPRKKNTQNRWKNVDRAFYQDVRLPPI
                     QLYKVGDIYFVKDGNHRVSVARERGVEFIDAEVIEGHVRVPLSSTMSTAELLLQTEYA
                     EFLRRTDLDRLQPDHDIRPTLLGRYDEIWDQIQGHRDWLSAIWHREATVHEAVNDWYH
                     YIYMPIIKVVRDRKVLEEFPALSEADIYLWVMRHRHLLEEREGHDVGPIASANAYAEL
                     MTQPMGVGDRVRTILGDNPREPDSEKEEKRRRRMRRWPRPRVGI"
     gene            complement(1026..1760)
                     /locus_tag="VFQ54_08980"
     CDS             complement(1026..1760)
                     /locus_tag="VFQ54_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0165167.1"
                     /translation="MDLDILAITDEVDQRIYSASVAQRMSNVQMVIGCGDLPANYLEF
                     LSDALNKPVYYVLGNHAEELTRAGDRGIPRHPEGCIDLGGKVITDPGTGLIMAGLPGS
                     LRYSMDEPVQYTEWQMTWMIWKMTPRLLWNRYRHGRALDLLVSHAPPRDVNDREDPAH
                     RGFVAMRRFLRRFHPAYQLHGHIHLYDRSKPNTARFDETEVINVYPYQNLTLSFAALD
                     DPHGTTTERSSHQTRSSGELEVEHRA"
     gene            complement(1845..2477)
                     /locus_tag="VFQ54_08985"
     CDS             complement(1845..2477)
                     /locus_tag="VFQ54_08985"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0165168.1"
                     /translation="MRLLLVRHGQSVGNIEQRIQGDDDPLTDRGREQAHAVADFLAKR
                     RDITHLYASPLARAIETARIIGTAIGRDPIPTPGLAEINTGSASGARWDEWNAMNPEK
                     AAALASFDEAWPGGESACDFSRRVLATYAGIIARHRGTGDVVTLVSHGGTLAWISARL
                     HGDPLDAWPAERAIFINCSVSEVRFDGDIPDFLDWNQHGHLEALLVEPPR"
     gene            2642..2896
                     /locus_tag="VFQ54_08990"
     CDS             2642..2896
                     /locus_tag="VFQ54_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165169.1"
                     /translation="MPSRDQRVDFRVVPIAWRCSPRQTQIPLKKRMKAKLMLARIAAW
                     TGPFGTRISAGPVPVVPSALDVLVLVQTVPAPVPSPPIAY"
BASE COUNT          524 a          908 c         1038 g          582 t
ORIGIN      
        1 atccttcgac aagctcagga tgacggtcag cgtgtagagc ggcaggcaca tcttccgacc
       61 cttgctctag atgcccacgc gtggacgcgg ccagcgccgc atgcggcgac ggcgcttctc
      121 ttccttctcg gaatccggct cgcgcggatt atcgccgagg atcgtccgca cgcgatcgcc
      181 gacccccatc ggctgggtca tcagttcggc gtaggcgttc gccgaggcga tcggaccgac
      241 gtcatggcct tcccgctcct cgagcagatg gcggtgtcgc atcacccaga gatagatgtc
      301 cgcctccgag agcgcgggga attcctcgag gaccttgcga tcgcgcacga ccttgatgat
      361 cggcatgtag atgtagtggt accagtcgtt gacggcctca tggacggtcg cctcgcgatg
      421 ccagatcgcg ctcagccagt cgcgatgccc ctggatctga tcccagatct cgtcgtagcg
      481 accgagcagc gtcggacgaa tgtcgtgatc gggctgcagg cgatcgagat cggtacggcg
      541 gaggaactcc gcgtattccg tctgcagaag cagttccgcc gtcgacatcg tcgaggagag
      601 gggaacgcgg acgtgtccct cgatcacctc ggcgtcgatg aactcgaccc cgcgctcgcg
      661 ggcgacggag acgcgatggt tgccatcctt cacgaagtag atgtcgccga ccttgtagag
      721 ctggatcggc gggagacgca cgtcctggta gaaggcgcga tcgacgttct tccagcggtt
      781 ctgcgtgttc ttcttgcgcg gcaggaaggt gcgatcgaaa tcctggaagc ggtccatgct
      841 gccgacgatc ttgtcgatcg gcacggtctg caggccgcga tagctctccc cttccgggtt
      901 gagccgcttc cggacttcgt ggaacgggat cagatcgttc tgccgaccgg tcgccgtggc
      961 aacgagatcg tgcaggaagg ctttctggcg ggcgcgttcg aaatcacttc gtagctgatc
     1021 ttcaatcatg cgcgatgctc cacctccagc tcgccagagc ttcgtgtctg gtgggatgaa
     1081 cgctcggttg tggtgccatg gggatcgtcg agtgcggcga acgagagggt gaggttctga
     1141 tagggataga cgttgatgac ctcggtctcg tcgaatctgg cggtgttggg tttcgatcga
     1201 tcgtagagat gaatgtggcc gtggagctga taggcgggat ggaaccggcg aaggaagcga
     1261 cgcatcgcga cgaaaccgcg gtgggcggga tcttcccggt cgttgacgtc gcgtggcgga
     1321 gcgtggctga cgagaagatc gagcgcccgc ccgtggcggt acctgttcca gaggagacgc
     1381 ggcgtcatct tccagatcat ccaggtcatc tgccactcgg tgtactgtac gggttcatcc
     1441 atggaatagc gaagtgaccc cggaagtccg gccatgatca gccccgtgcc gggatcggtg
     1501 atcaccttgc caccgaggtc gatgcatcct tccggatggc gggggatacc acgatcgccg
     1561 gcccgggtca gttcctcggc gtgattcccg agcacatagt agacgggttt gttcaaggcg
     1621 tccgagagga actcgaggta atttgccggg agatcgccgc acccgatcac catctggaca
     1681 ttgctcatgc gctgggcgac cgaggcgctg tagatgcgct gatcgacttc gtccgtgatg
     1741 gcaagaatat ccagatccat acctgtcttt ccacaaacat atgggcagtg gcggctgtcg
     1801 aggcagcgga cgtggatgac cgccccgccg ctatcatact gcaatcatcg gggaggctcg
     1861 accaggagcg cctcgagatg accgtgctgg ttccagtcaa gaaaatcggg gatatcgccg
     1921 tcgaaccgga cttcgctgac ggagcagttg atgaagatcg cgcgctctgc cggccaggca
     1981 tcgagcgggt cgccatgcag ccgggcggag atccaggcga gcgtaccacc gtgggaaacg
     2041 agggtgacga cgtcgcccgt gccccgatga cgagcgatga ttccggcgta ggtggcgagg
     2101 acgcgtcgcg agaagtcgca ggcactttcg ccgcccggcc aggcttcgtc gaatgacgcg
     2161 agtgccgccg ccttctcggg gttcatcgcg ttccactcat cccagcgggc gccggaggcg
     2221 ctgccggtgt tgatctcggc gagaccgggc gtcgggatcg gatcgcgtcc gatcgcggtg
     2281 ccgatgatcc tggcggtctc gatggcgcgg gcgagcgggc tcgcatagag gtgcgtgatg
     2341 tcccggcgtt tggcgaggaa gtccgccacc gcgtgggcct gctcgcgacc gcgatcggtc
     2401 agcggatcgt catcgccctg gatgcgttgc tcgatatttc caaccgattg accgtgacgt
     2461 acgagcagca atcgcatcgc cttcgcaccc cttcgtgacg ttcccatccc tatgagagat
     2521 tgtacgcgga cgcaccaaag gcgatgcagc ttggcttctg catcgccttc ggtggagatg
     2581 ggtgctggtg gactcgtcct ggcgggctgg ccaggatgcg cggaccaggg gaagaggtca
     2641 catgccgagt cgggaccagc gcgtcgattt tcgtgttgtg ccgattgcct ggcgctgttc
     2701 gccgcgccag acccagatac cgctgaagaa gagaatgaag gcaaagctga tgctggcgag
     2761 gatcgccgcc tggaccgggc cgttcgggac gaggatttcg gccgggccgg tgccagtggt
     2821 gccgagcgcg ctcgatgtgt tggtgctcgt ccagacggtt ccggcgccgg ttccgtcgcc
     2881 gccgatcgct tactgagcgc acccgactcg gccctgctcg agtcgctcag catgcagtgg
     2941 ctggccatgg agtccccgac cggcctcgag cgcggcacgg acgcgaactg cgcggcaccc
     3001 gtgggcaccg gcccgttcgt ggtcgagagc tggacgccgc agcagcaggt cg
//
LOCUS       DASTMD010000609         1646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1217363, whole genome shotgun sequence.
ACCESSION   DASTMD010000609 DASTMD010000000
VERSION     DASTMD010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217363"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..781)
                     /gene="serB"
                     /locus_tag="VFQ54_08995"
     CDS             complement(143..781)
                     /gene="serB"
                     /locus_tag="VFQ54_08995"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEU0165170.1"
                     /translation="MARFLVVLDVDSTLIENEVIELLAEEAGTLELVASITADAMNGL
                     LDFEQSLRRRVSVLAGLTETDLAAVRARVEVTAGVPELIEAVHSAGGRVGVVSGGFHE
                     VIDPIARDLKLDYWRANRFAMDDGVHISGLEGPVIDAEAKAATLREWAADFGIPLAQT
                     VAVGDGANDLLMMAITGLAVGFDAKAPVRDEADVLIDVRDLSQLLPLLGLRG"
     gene            complement(833..>1646)
                     /locus_tag="VFQ54_09000"
     CDS             complement(833..>1646)
                     /locus_tag="VFQ54_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU0165171.1"
                     /translation="AGAGVTVAAIRQPIELADQFGVIELDTVDPTRIAAFHEKPKNAV
                     GLADSPREVLASMGNYVFDADILIEAVIRDGENPDSNHDMGGDIVPDFVARGEAAVYD
                     LNRNEVPGSTDRDKFYWRDVGTIESFFDAHQDLISALPIFNLYNREWPIFSQQLNSPP
                     AKFVRDAKGNLGTTIDSIVSLGTLVSGGHLERSVVGSWCVIDSGATVVDTVVFDRVRI
                     GPNAVVRRAILDKDVVISEGATVGVDHDADRARGFTVTDSGITVVGKGVQVD"
BASE COUNT          310 a          539 c          529 g          268 t
ORIGIN      
        1 tttgaacagg agcaagagca gcattagcgt gagacacgaa gacctccggg acgggatcgg
       61 aacctagaca gctccaccct ccaccggagg tcttcgtcac ttcaagacat cacacatcaa
      121 ccaacgtccc tgagcagtac agctagccgc gcaggccgag gaggggcagc agctgcgaga
      181 ggtcgcgaac gtcgatgagc acgtcggcct cgtcgcgaac gggagccttg gcgtcgaagc
      241 cgacggcgag gcccgtgatg gccatcatga gcaagtcgtt cgctccgtcg cccactgcga
      301 ccgtctgcgc aagcggaatt ccgaaatcgg cagcccactc gcgcaacgtc gctgccttcg
      361 cctcggcgtc gatgacgggc ccttcgagac cgctgatgtg cacgccatca tccatcgcga
      421 accggttcgc gcgccagtag tcgagcttca gatcgcgtgc gatgggatcg atgacctcgt
      481 ggaagccccc ggagaccacc ccgacgcgac ctccggcgga gtgcacggcc tcgatcagct
      541 cagggactcc tgccgtgacc tccacccggg cgcgaaccgc ggccaggtcg gtctccgtga
      601 gaccggcgag cacggagacg cgccgacgga ggctctgctc gaagtcgagg aggccgttca
      661 tggcgtccgc ggtgatcgac gcgaccagct ccagcgtccc tgcctcctcg gcgagaagct
      721 cgatcacctc gttctcgatc agggtcgagt ccacatcgag cacaacgaga aatcgagcca
      781 tccggcgatg ccttccatag ccaagggtcc tgtggtccga tgctggatcg cctcagtcga
      841 cctgaacgcc cttgccgacg accgtgatgc cggaatcggt gacggtgaac ccccgcgcgc
      901 ggtccgcatc atgatcgaca ccgaccgtgg cgccctcgga gatgacgacg tccttgtcta
      961 ggatagcccg gcgaacgacc gcgttcggcc cgatccgcac gcggtcgaac acgacggtgt
     1021 cgaccaccgt cgccccggag tcgatgacgc accacgaacc gacgacgctg cgttcgagat
     1081 ggccgccgga gacgagggtg ccgagggaga cgatcgaatc gatcgtcgtg ccgaggttgc
     1141 ccttggcatc gcgaacgaac ttggccggtg gtgagttcag ctgctggctg aagatcggcc
     1201 actcccggtt gtacagattg aagatcggga gcgcggagat gagatcctgg tgcgcgtcga
     1261 agaacgactc gatcgttccg acatcgcgcc agtagaactt gtcgcgatcg gtcgagcccg
     1321 gcacctcatt gcggttgagg tcatagacgg cggcctcgcc gcgcgccacg aaatccggaa
     1381 cgatgtctcc gcccatgtcg tggttggaat ccggattctc gccgtcgcgg atcactgcct
     1441 cgatcaggat gtcggcgtcg aagacatagt tgcccatcga ggcgagaacc tcgcgggggg
     1501 agtcggccag accgacggcg ttcttcggct tctcgtggaa cgcggcgatt ctcgtcggat
     1561 cgaccgtgtc gagctcgatc acgccgaact ggtcggcgag ctcgatcggc tggcggatcg
     1621 ccgcgaccgt cacaccggcg cctgcg
//
LOCUS       DASTMD010000610         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1487270, whole genome shotgun sequence.
ACCESSION   DASTMD010000610 DASTMD010000000
VERSION     DASTMD010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487270"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFQ54_09005"
     CDS             complement(<1..337)
                     /locus_tag="VFQ54_09005"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0165172.1"
                     /translation="MLELGAGRRAAIRDLDAVVARLHELAPEDWERDTPDAGWKVRHL
                     AAHLTTTIPFVTTVLGQVIAIRLGHLPTMETGIEVTPDSDRHDITASVTQHRNAFVHT
                     ISAMAEDDLT"
     gene            complement(427..1545)
                     /locus_tag="VFQ54_09010"
     CDS             complement(427..1545)
                     /locus_tag="VFQ54_09010"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU0165173.1"
                     /translation="MRRATFGDPRRFTRRERVRWIAPWAIVAAVGGSCDLAGVPVAWL
                     IGPMLASVVLSIRGLPPTPNPSLVFTGVQAIIGTTLSASFTVDSLKPMAAHWLPVMIS
                     VVLVLAFSIAGGQLLSRIAPIDAATASLGTVPGGASGMVAMSEDLGGDARLVAFMQYL
                     RLIVVVISISLSARLIDHDASVVQQVVDDGDAWPARASCLLAIAVSIAGGWLGLRLRI
                     PAGALLGSMMVGLIPGGLDIGPVAWPPFVLPVAYLLLGARVGSRFDGDVLRRIRTLLP
                     YIIGFVLGLCLLCAGIGWTLAATTDIDLLTALLATSPGGIDSATIAALATGANVPMVL
                     SMQIVRLLIMVIAGPFLIRRLVPARVIATIPAEPPVRP"
     gene            complement(1769..>3312)
                     /locus_tag="VFQ54_09015"
     CDS             complement(1769..>3312)
                     /locus_tag="VFQ54_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165174.1"
                     /translation="QRLADRTERDETKASARSKAGHAARPKADRGNDSSPVFSLPDGG
                     DNATLEATAPDTGNDSETVEWGGEDADTDTSVEPGNTGSIVDDAHTNNGSSGSVASND
                     PPTNNGGQTSVAPANTTYNQSKTTTTEIDSTVEEDGVRTERPLGTDRFGEEIEPDTTR
                     SQALAIRAFEEANDRNATAAERRQLRQLAEWAATTGFSGGYDAWRIVTSAIEEAVAAG
                     SSFVAPKRIREILNRWSRDGVPAEYVDGVRVRGVAGMAQDGPVTSEQDASHRPVPDAA
                     RALQFPNGKSGADVWGSVEQAVRPSLGVAPTRELFAGTAIIGYDAGEVTILVATASQH
                     EAFALTHHDLVIRKLGSALRRPIRLAVVVAAGDPDPATEAHDGKTPDASIPAPRRELP
                     PRPAPHVQTLSAMPVFTIERSGMTNRQIWALALTDLREGGVVPRQDIEAWLRDVVILA
                     VDDDAGTIGVRLGVPHVLAARRIEGRFLPAIRQALGRLLETSAGGHEIVIDVTLTRDW
                     LRETA"
BASE COUNT          597 a         1114 c         1047 g          554 t
ORIGIN      
        1 cggtgagatc atcctccgcc atcgccgaga tcgtgtggac gaaggcgttc cgatgctggg
       61 tgacgctggc ggtgatgtcg tgacgatcgg agtccggcgt cacctcgatc ccggtctcca
      121 tcgtcggcag atgcccgagg cggatcgcga tcacctgccc aaggacggtc gtgacgaagg
      181 ggatcgtcgt cgtcagatgc gcggcaagat gccggacctt ccagcccgcg tccggggtgt
      241 cgcgttccca atcctccggc gcgagctcat gcagccgagc cacgaccgca tcgagatcgc
      301 gaatcgccgc gcggcgtcct gctccaagtt ccagcatgct gacgttccct ctctctgcgt
      361 tcgcaatccg acgacgatgg atgtacggac agagtataga acatgcgtcc tactccattg
      421 ccaccgtcac ggtcgcaccg gtggctcggc agggatcgtg gcaatgacgc gcgcaggtac
      481 gaggcgccgg atcaggaagg gaccggcaat caccatgatc aggagccgca cgatctgcat
      541 ggaaagcacc atcggcacgt tggcgcccgt cgccaaggcg gcgatcgtcg ccgaatcgat
      601 cccgccgggg gaggtcgcga ggagcgccgt caggagatcg atatccgtcg tcgcggcaag
      661 tgtccagccg atccctgcgc agagcaggca caacccgagg acaaagccga tgatgtatgg
      721 gaggagggtc cggatgcgtc tgagcacatc cccatcgaac cgggacccga cccgcgcccc
      781 gagcagcaga tacgcgaccg gcagcacgaa cggcggccag gcgaccggtc cgatatcgag
      841 cccccccggg atcaggccaa ccatcatcga cccgagcaac gccccggccg ggatgcgcag
      901 gcgcaacccc aaccagccgc cggcgatcga gaccgcgatc gcgagcaggc aacttgcccg
      961 ggccggccag gcgtcgccat catcgacgac ctgttgcacg accgatgcgt cgtgatcgat
     1021 cagccgcgcc gagagggaga tcgagatcac gaccacgatc aggcgcaggt actgcatgaa
     1081 cgcgacgagc cgcgcatccc cgccgagatc ctcactcatc gcgaccatcc cggacgcacc
     1141 gccggggacg gttcccagcg aggcggtcgc ggcatcgatc ggagcgatcc tcgagagaag
     1201 ctggccaccg gcaatgctga aggcgaggac aaggacgacc gagatcatca ccggcagcca
     1261 atgggccgcc atcggcttca gcgagtcgac cgtgaagctg gcgctgagcg tcgtcccgat
     1321 gatcgcctgg acgccggtga agacgaggga gggattcggt gtcggcggca gcccgcggat
     1381 cgagaggacg acggaggcga gcatcggtcc gatcagccag gcaaccggga caccggcgag
     1441 atcgcaactg ccgccaaccg cggccacgat cgcccacggt gcgatccacc gcacccgttc
     1501 gcgccgggtg aagcgacggg gatcaccgaa cgttgcacgt cgcaatcgcg atcctgggga
     1561 cgagggcact ggcggttctc cggttccgac tggtggagat ggccgctcgg cttccgtgag
     1621 ccgcgaccat ctccatcgtt tcaacctgcg aatcgggaag ccgccacatc gcggtatgtc
     1681 cgaagcgaac cctcggacca ttcaactcct tgcggataat tcttgaatta cgcgggttgt
     1741 ccgccagaaa acacctggcg agaacgtatc aggccgtctc gcggagccag tcgcgggtca
     1801 gcgtcacgtc gatgacgatc tcatgaccac cagcacttgt ctcgaggaga cgaccgagtg
     1861 cctggcggat cgccggcagg aatcgccctt cgatccgtcg cgcggcaagc acgtgcggca
     1921 ccccaagcct gacaccaatc gttcccgcat catcgtcaac ggcgaggatc acgacatcgc
     1981 gcagccacgc ttcgatgtcc tgacgaggca ccaccccgcc ctcgcggaga tcggtcagcg
     2041 cgagggccca gatctggcga ttcgtcatcc cgctccgctc gatcgtgaag accggcatgg
     2101 cgctcagcgt ctgcacgtgt ggcgccgggc ggggcggcag ttcgcggcgc ggtgccggga
     2161 tcgacgcgtc cggggtcttg ccatcatggg cttccgtggc tggatccgga tctcctgccg
     2221 cgacgaccac agccagccgg atcggtcgcc gcagcgcgct cccgagcttg cgtatcacca
     2281 gatcgtggtg agtgagcgcg aaagcctcat gctggctcgc ggtcgcgacc aggatcgtga
     2341 cctcaccggc atcgtagccg atgatcgccg ttcccgcgaa gagttcccgc gtcggcgcaa
     2401 ccccgaggct cggccgtacc gcctgctcga ccgatcccca gacatcggcc ccgctcttgc
     2461 cattcggaaa ctggagcgcc cgcgccgcat ccggaaccgg cctgtgactg gcatcctgct
     2521 cgcttgtcac aggtccatcc tgggccatcc cggcgacacc ccgaacgcgt acgccgtcga
     2581 cgtactcggc cggaactccg tcgcgagacc agcggttcag gatctcccgg atccgtttcg
     2641 gagcgacgaa cgaactgccc gccgcaaccg cttcctctat cgcggacgtg acgatccgcc
     2701 aggcatcata ccctccggaa aagcctgtcg tggcggccca ttcggcgagc tggcgaagtt
     2761 gccggcgttc cgccgcggtc gcattgcgat cgttcgcttc ttcgaaagcc cggatcgcca
     2821 gtgcctgcga gcgcgtcgta tcgggttcga tctcctcgcc gaagcgatct gtcccgagcg
     2881 gtcgttctgt acgtacaccg tcttcttcga ccgttgaatc gatctcggtt gttgttgttt
     2941 ttgattggtt atacgtcgtg ttggcgggag caacactggt ttggccacca ttgttggtcg
     3001 ggggatcatt gctcgctacg gatccactcg aaccattgtt ggtgtgtgca tcatcgacga
     3061 tcgaaccagt gttgcccggt tcaacagagg tatcggtatc ggcatcctcg ccaccccact
     3121 cgacagtctc gctgtcattg ccggtgtcgg gtgctgtcgc ctccaatgtc gcgttgtcac
     3181 cgccatctgg caaagaaaaa acagggctgc tgtcattccc gcgatccgcc ttgggacggg
     3241 cagcatggcc agccttgctc cgggccgatg ccttcgtctc gtcgcgctcg gtacggtcgg
     3301 caaggcgctg cc
//
LOCUS       DASTMD010000611         2612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_770523, whole genome shotgun sequence.
ACCESSION   DASTMD010000611 DASTMD010000000
VERSION     DASTMD010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770523"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFQ54_09020"
     CDS             <1..432
                     /locus_tag="VFQ54_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0165175.1"
                     /translation="DRFVDLMEPSLADDDPADAAFIRSQIVEIRQRLRDLDPGEGGWG
                     LIHGDVQELNFHFTAENQITFFDFDLCGYGWRAYEISYYYTRIREELRAPVIAGYESV
                     RPLSVSEREMLPTFGKLAWIREGLMSRDLIEKLHDPYMKFD"
     gene            complement(477..1724)
                     /locus_tag="VFQ54_09025"
     CDS             complement(477..1724)
                     /locus_tag="VFQ54_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0165176.1"
                     /translation="MSEAELRSHFDIHSAFADGVPYDQYAWYREHDPIHVGEPREWAT
                     PIYLFRHADVIKLLRDQRLGKEWRKLMPPDAEIPTPEPDSFMAVAGKFMLFRDPPTHT
                     HLRSLANLAFTPRQVEQMRPHIKELAAKLVAEFKDHPADGPADLIMNVAYPLPTLVIA
                     KILGIPSEDYGRFRQWAGDIAAAIDFPLEGLADFVARVDQTTKELSEYLRWVFQLRRD
                     DPQDDLISRLIQARTDEGTLDEDEVIGTCILLLVAGHETTVNLIGNGTLALLRHRDQW
                     QRLVEDPGLARTAAEELLRYDSPVQMTTRVSYEAIDLDGTQIPAGTEVNFMLGSANLD
                     DAAFTDAARLEIGRDVGRIMSFGMGIHFCLGAPLARLEGEVAFETIARELPGMTLATD
                     RPVWRPGAVLHGLRELPVHIGDA"
     gene            complement(2179..>2612)
                     /locus_tag="VFQ54_09030"
     CDS             complement(2179..>2612)
                     /locus_tag="VFQ54_09030"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU0165177.1"
                     /translation="DGLKSIGSAGITALGFQSSVFDWLGEIAAHEAAHVETLTKVIGG
                     LGGTPVAEATYDFGYTDAAGFLGVAMAIEDTGVSAYGGAAQYLMDNGDLLTAALTIHG
                     VEARHASYLAVLNAKNPFPNAFNPALTPDKVLDIAGPFIKS"
BASE COUNT          481 a          811 c          842 g          478 t
ORIGIN      
        1 gatcgattcg tcgatctgat ggagccgtca ctcgccgacg acgatccggc cgatgcggcg
       61 ttcattcgaa gccagatcgt cgagatccgg caacgactgc gtgacctcga tccgggcgag
      121 ggcggctggg gactgattca cggggacgtc caggagttga acttccactt caccgccgag
      181 aaccagatca ccttcttcga tttcgatctc tgcggatatg gctggagagc ctacgagatt
      241 tcgtactact acacgcggat ccgggaagag ctccgggcac cagtcatcgc gggatacgag
      301 tcggtgaggc cactgagtgt gtccgaacgc gagatgcttc cgacgttcgg caaactggcg
      361 tggattcgcg agggcctcat gtccagggat ctgatcgaga agctccacga tccgtacatg
      421 aagttcgact gatcgtgtga tttcaccacg agttgcggtg ttcagcggaa ctcgtatcag
      481 gcgtcgccaa tgtggacggg cagctcgcga agcccgtgaa ggacggcgcc cggacgccag
      541 acggggcgat cggtcgcgag cgtcattccc gggagttcac gagcgatcgt ctcgaaggcg
      601 acctctccct cgaggcgggc cagcggcgcg ccgaggcaga agtgaattcc catcccgaac
      661 gacatgatcc gaccgacatc gcgtccgatc tcgagacgag ccgcatcggt gaaggcggca
      721 tcgtccagat tcgcggagcc aagcatgaaa ttgacctccg ttcccgccgg gatctgcgtt
      781 ccgtcaaggt cgatcgcttc ataggaaacg cgagtcgtca tctggaccgg gctgtcgtac
      841 cggagcagct cttctgccgc cgtcctcgcg agaccgggat cctcgacgag ccgctgccat
      901 tgatcgcgat gacgcaggag ggcgagggtg ccgttcccga tcagattgac cgtcgtttcg
      961 tggccggcaa cgaggaggag gatgcaggtg ccgatcacct cgtcctcgtc gagcgtcccc
     1021 tcgtcggtgc gcgcctggat cagccgggag atcaggtcgt cctggggatc gtcccgccgc
     1081 aactggaaga cccaacggag atactcggag agttccttcg tcgtctgatc gacccgggcg
     1141 acaaaatccg cgagtccttc cagcggaaag tcgatcgcgg ccgcgatatc cccggcccac
     1201 tggcggaaac gcccgtagtc ctcggacggg atgccgagga tcttcgcgat caccagggtt
     1261 ggcagcgggt aggcgacatt catgatcaga tcggcgggac cgtccgccgg atggtccttg
     1321 aactcggcga cgagctttgc ggcgagttcc ttgatgtgcg gtcgcatctg ctcgacctgc
     1381 cgcggcgtga aggcgagatt cgcgaggctc cggaggtgcg tatgcgtcgg cggatcgcgg
     1441 aagagcatga acttgccggc gacggccatg aaggagtccg gctccggcgt cggaatctcg
     1501 gcgtcgggcg gcatcagctt gcgccactcc ttgccgagcc gctggtcgcg caggagcttg
     1561 atgacgtcgg cgtgacggaa gagatagatc ggtgtcgccc actcgcgcgg ctcgccgacg
     1621 tgaatcgggt cgtgctcccg gtaccaggcg tactggtcgt acggaacgcc atccgcgaag
     1681 gcggagtgta tgtcgaaatg actccgaagc tctgcctcac tcatcgcgcg acaccctcct
     1741 gcctgatcga tacttcgagc gaaagcgacg tcgccaaggg aacggtcacc cggcgaagaa
     1801 cgggatcgct cgatccttct tctatataaa gggcattcta cacgtcaccc cgtccactct
     1861 tgacgcctca acgccaacgg ccccgttcca ggggaacaag ggccgtcgga cattcttgtg
     1921 cgaaccgtgt cggacgtacc gcgaaaacga aacgattgct acgggtgccg ccggatcgga
     1981 ggccacgtcc cccacgacat ccggttgccc gaggatcgag ctccccgcgg caattgtctc
     2041 gatcgctgct ctcgtggtct gtcactggcg tcttgctggt tggcatcgta tgccaaccgt
     2101 catatgcggc ttcgccgcat gggccacacg gggtggcccg ctacggaatc gccgtggcaa
     2161 ttcaggcgtg acgcgccact agctcttgat gaacggtccg gcgatgtcga gcaccttgtc
     2221 cggcgtgagg gccgggttga atgcgttcgg gaacggattc ttggcattca gcacggcgag
     2281 atacgaagcg tggcgggcct cgacaccgtg gatcgtcagc gccgccgtca gcaggtcacc
     2341 gttgtccatc aggtactgcg ccgcaccgcc gtaggcggag acacccgtat cctcgatcgc
     2401 catcgcgacg ccgaggaatc ccgcggcatc ggtgtaaccg aagtcgtagg tcgcctcggc
     2461 aaccggcgtg ccgccaagtc cgccgatcac cttcgtcagc gtctcgacgt gtgcggcctc
     2521 atgcgcggcg atctcgccga gccaatcgaa gacgcttgac tggaagccga gggcggtaat
     2581 gccggcgctg ccaatcgact tcagaccgtc gc
//
LOCUS       DASTMD010000612         1811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2378936, whole genome shotgun sequence.
ACCESSION   DASTMD010000612 DASTMD010000000
VERSION     DASTMD010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2378936"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFQ54_09035"
     CDS             <1..217
                     /locus_tag="VFQ54_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165178.1"
                     /translation="YFNTDPHGNDVVIFNGVADGDEKSLDPTDAAAYLFKYRSGIEGL
                     GMSPESFANDYSRVITVDLTKVRGWIG"
     gene            364..1266
                     /locus_tag="VFQ54_09040"
     CDS             364..1266
                     /locus_tag="VFQ54_09040"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU0165179.1"
                     /translation="MNPRTLLRPGDTDVAPRFPSLRRFPRLFLFGVSQVAVLLVMFQV
                     YKIFRKTFIGHAENVAFGHAQDVLSFESRLHLEFERPLQKFVLDRPDWVIKAFNQVYA
                     NYMWIFFFGMVVGMFFAVERWRYYRRWFFISMAIATPWYAIYPLAPPRFMQPYGYDFV
                     DTLARYGPNYFSDNGFVAANRYAAMPSMHVGWTTLAAIVIANCIPWKKTGKVIAGFLI
                     VLITLTVMVTGNHYWLDAVAGWTVITVAALINRALPYPFPIPWPWVHPQAREVPALAP
                     AMPVAGPTPAASRPQMQEPSGSHD"
BASE COUNT          367 a          569 c          512 g          363 t
ORIGIN      
        1 ctacttcaac accgatccac acggcaacga cgtcgtgatc ttcaacggcg tggccgatgg
       61 cgacgaaaag tcgctcgatc ccaccgacgc ggcggcatat ctcttcaagt accgctcggg
      121 catcgagggt cttgggatgt cgcccgagtc gtttgccaac gactacagcc gcgtgatcac
      181 ggttgatttg accaaagtcc ggggctggat cggctgacga acgggtactg cctctcgaag
      241 catcgaatcc aggccaacat gttgttaata tgttggcctg gatcgtgccc attgagggac
      301 acgtatcagg atgcgtgagt aacggcgcac caagcgctca tcacgctctc gtaaggtcag
      361 cacatgaatc cacgtacgct cctgcggccc ggcgacaccg atgtcgcacc ccgcttcccg
      421 tcactgcgac gcttccccag gcttttcctc ttcggggtct cccaggtcgc cgtcctcctg
      481 gtcatgttcc aggtctacaa gatcttccgg aagaccttca tcggccacgc cgagaatgtt
      541 gcgttcggtc acgcccagga tgtcctgagc ttcgaatcgc gtctccacct cgaattcgaa
      601 cgtcccctcc agaagttcgt cctggatcgg cccgactggg tgatcaaggc cttcaaccag
      661 gtctatgcga actacatgtg gattttcttc ttcggcatgg tggtcggcat gttcttcgcg
      721 gtcgaacgct ggcgctacta ccgtcgctgg ttcttcatca gcatggcgat cgcgacgccg
      781 tggtatgcga tctatcccct cgccccaccc cgcttcatgc aaccgtatgg gtatgacttc
      841 gtcgacacgc tggcgagata cggaccgaac tatttcagtg acaacggctt cgtcgccgcg
      901 aatcgttacg ccgcgatgcc gagcatgcat gtcggctgga cgacgctggc cgcgatcgtc
      961 atcgcgaact gcatcccatg gaagaagacg ggcaaggtca tcgccggctt cctgatcgtg
     1021 ctgatcacac tgacagtgat ggttaccggc aatcactact ggctcgacgc cgtcgcgggc
     1081 tggaccgtga tcacggtggc ggcgctgatc aaccgggcgc tgccctatcc attcccgatc
     1141 ccctggccgt gggtgcaccc ccaggcgcgg gaagtcccgg ccctcgctcc ggcgatgccg
     1201 gtggccgggc caacgccggc ggcgagtcgg ccccagatgc aggagccgag cggatcccac
     1261 gactgaccgt gcgctggatc cagtggttta aggaaaatag cgttacacct attgacagag
     1321 gcacccggag cccttatgat ccagtggtgc gcggagagat cgctccgcaa cgatgggtga
     1381 aggcatggat gcctcctcat cgaacgacag atagcagcaa cgaccacagg gtcacggaaa
     1441 ggaggtatcg gcatgttcat gatgattggc gcgaatcgcg cctggatcgc ccctcgaact
     1501 ggcgatatcg actcaatccg tggcaattac acccgcatca gggagctatc gatcactcga
     1561 tagccgagat gtagaacgtg gtccagcccc gggttgaaat caacccgggg ctttttgcgt
     1621 gtcccgctca acggacacat gcagcagccg gtccggcatc gcctccggga atcgacagat
     1681 cagccatcgg gcactacgtc gaagaaacac agagcacacg ctctgtgacg gcatcttgct
     1741 gtcttttcat gaaacatcga ggagatacat atgaagccgt cacccgacgc cacaagatac
     1801 gcctcgccat c
//
LOCUS       DASTMD010000613         4399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1844627, whole genome shotgun sequence.
ACCESSION   DASTMD010000613 DASTMD010000000
VERSION     DASTMD010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1844627"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFQ54_09045"
     CDS             <1..499
                     /locus_tag="VFQ54_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165180.1"
                     /translation="AVAPGTTDPASRIFELKVHRSEGTLHERGDLFHLVKVMDLAGPE
                     HFYHDVLGMELLARTRMSDGRLVELGADYDHATAAQTNTEADFAFLAHGPVKLALQRT
                     GRASILDYGKIVNDFALVVEPSLAKRIKAQVLMRGFTLLEEKPASFTLRDPYAVAWEI
                     VPAVG"
     gene            563..1009
                     /locus_tag="VFQ54_09050"
     CDS             563..1009
                     /locus_tag="VFQ54_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165181.1"
                     /translation="MLDSYRDLIDGLLATPSALRLALGDPVPESVSPAVLQLLVELRA
                     RELVMLRRARSIMRAESAALRDVMDEPELKAAHAGEATEATPEELIAGFNTDRSELVS
                     LLMNVTIREWERPVDHHVNGETTLAEEIDDHLTWDEDVIARIRAAA"
     gene            1623..1931
                     /locus_tag="VFQ54_09055"
     CDS             1623..1931
                     /locus_tag="VFQ54_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HEU0165182.1"
                     /translation="MTEQQSQLQPRHGEYKMPGGKLVVVDFAVEAGELRRVMVSGDFF
                     LYPDSTLADISAALEGITATSSEADVARHVRDGLAPGAELIGVTPEGIGVAVRRALDA
                     "
     gene            1973..2824
                     /locus_tag="VFQ54_09060"
     CDS             1973..2824
                     /locus_tag="VFQ54_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEU0165183.1"
                     /translation="MREVMEGGGETAEPTVTELTGLEPDALTQRFAAQQWRLLPLTPL
                     SPMMQTALDETLTLRVGRGQRGPTLRFWRWSGPCVVLGRFQSVRNEVNDDVLRANGFD
                     LVRRISGGGAMFIQPEGAITYSIYAPEEIASGMSFPQSYAFFDSWVIEALRELGVEAR
                     YAPLNDITSVNGKIGGAAQARRGGAILHHTTMAYQINGPLMSQILRVGKEKLSDKGVT
                     SADKRVGPLRQQTDLERGAIIDHLIAHFRRRFGLIDDEFTPEELDEGAEAVRTRFGTR
                     EWVYFLP"
     gene            3341..>4399
                     /locus_tag="VFQ54_09065"
     CDS             3341..>4399
                     /locus_tag="VFQ54_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0165184.1"
                     /translation="MVALCALLLVGCGSAGGDDIAWHPVREAVSTVVATPAPPTATDA
                     PAVAQEFTHQLSAAELQKYQPDELGLVPVPMYHAFVKDDESKDTGGGGDSLDEWTRTL
                     SEFRSDLQWYYDHDFYVVPMRDYVENDMKVPAGKHPMVLTFDDASTRQAMWIKNKKGQ
                     IVADPDSALGVMEDFYAKHPDFGRGGYFAVLIFNCFNNPDGPPEMADCATKLNWLIDH
                     GYEVGNHTWGHQDLTDITDDEFLYQVGKPILWFRQNIEDKGDMSEVLTLPYGAYPDRA
                     ETQQWDYLVNGFDYHGERVDLEGILQVNGGPALSPSSDKFDRYGIARFNTDPDVMAYW
                     WKRIEDGELTMYTSDGNPN"
BASE COUNT          824 a         1342 c         1443 g          790 t
ORIGIN      
        1 tgcggtcgcc ccgggaacga cggaccccgc ctcccggatt ttcgaactga aggtccatcg
       61 ttcggaggga acgctccacg agcgggggga cctcttccac ctcgtgaagg tgatggatct
      121 ggccggtccg gagcatttct atcacgatgt gctcgggatg gagttgctgg cccggacgcg
      181 gatgtccgac ggtcggctgg tcgagcttgg cgccgattac gaccacgcga cagccgcgca
      241 gacgaatacg gaagccgact tcgcattcct ggcgcacgga ccggtgaaac tggcactgca
      301 gcgaaccggg cgcgcgagca tcctcgacta tggcaagatc gtcaatgact tcgcgctcgt
      361 cgtcgagccg tcgttggcga agcggatcaa ggcgcaggtc ctgatgcgag gatttaccct
      421 gctcgaggag aaaccggcat cattcacgct ccgcgatcca tatgcggtcg cctgggaaat
      481 cgttcccgcg gtcggctgat cccctcgtta gaagagtccg cccgcgatcc atcgggctgg
      541 atgcaatcag gaagttccat acatgctcga tagttatcgc gatctgatcg acgggctcct
      601 cgcaacgcca tctgcgttac gtctggcgct cggcgatcct gtgccggaat cggtctcgcc
      661 ggccgtgctt cagctccttg tcgaactgcg ggccagggaa ctggtgatgc tccgtcgcgc
      721 ccggtcgatc atgcgggccg aaagcgccgc cctccgcgat gtgatggacg agcccgagtt
      781 gaaggccgct cacgctggag aggcgactga agcaacgccg gaagagctca tcgccggctt
      841 caacaccgat cggagcgagc tggtctcgct gctcatgaac gtcacgatcc gtgaatggga
      901 gcgcccggtc gatcatcacg tcaatggtga gacgacgctt gccgaggaga tcgacgatca
      961 cctgacctgg gacgaggacg tgatcgcccg gatccgcgcc gcggcgtagc caggaggcgg
     1021 gatcgagaag atcccgtacg aacggacggg tttgccgaca acgagcgttt gttgaaccgt
     1081 ggatgcgact ccatttgcct ctgctcggga caatcccttg tggtcgctcg ggccggcgtc
     1141 gccgatccgg aatccgcggt ggacgaccag gtcgcatcgg gcccatctgc cgcgagagca
     1201 aaggggagag cccctgccgg atatccggca ggggctcaga gggaggggga tgagggggtc
     1261 aacgacccgt gaatcgcgcg gctgggggac cgcaaggcga cccggggtgc tgatgaacct
     1321 agattagcac atatgttcga acatggcaac cggatgcgac tggctggatt cgcaggcgga
     1381 gtcattgcgg agcatggctt ggcaagccgt gcccggccgt tcgaaactga cgcgttcggt
     1441 tggcacagta tcgcgcccct tcatcagtaa gaacgcacga cgtcggaaaa tgtttcatat
     1501 atctcggaga ttttctggag ttcgctccca gcatccggct tcatggcgag gtcttcgatc
     1561 tggacgccat cgcatgggcg cggcatcatg ccttcgtaac gatcgaccag gagtgagccg
     1621 ggatgacaga acaacaatca caattgcaac cgcgacatgg tgaatacaag atgcccggcg
     1681 gcaagctcgt cgttgtcgac tttgccgtcg aggcggggga gcttcgccgg gtgatggtga
     1741 gtggtgactt cttcctctat ccggatagca cgcttgccga tatttctgcc gccctcgaag
     1801 ggatcacggc gacctccagc gaggcggacg tcgcgcgcca cgtccgtgac gggctcgcac
     1861 caggcgccga actcatcggg gtcacgcccg aaggaatcgg cgtcgccgtg cgccgggcgc
     1921 tcgacgcctg aaccgcgagg tcgagataga gatggatcaa aggggaaccc ctgtgcgtga
     1981 ggttatggaa ggtggcggcg aaacggcgga gccgaccgtc acggaattga ccggcctgga
     2041 gccggatgcg ttgacgcagc ggttcgccgc gcagcagtgg cggttgctgc cgctgacccc
     2101 gttgtcgcca atgatgcaga cggcgctcga cgagacgttg acgctgcgag tcgggcgtgg
     2161 ccagcggggc ccgacactcc ggttctggcg ctggagcggc ccctgcgtcg tgctcgggcg
     2221 cttccagtcg gttcgcaacg aagtgaacga tgatgtgctc cgcgcgaacg gcttcgatct
     2281 cgtgcgccgg atcagtggcg gcggggcgat gttcatccag ccggagggcg cgatcaccta
     2341 ttcgatctac gcgccggagg agatcgcgtc ggggatgtcc ttcccgcagt cgtacgcctt
     2401 cttcgattcg tgggtgatcg aggcattgcg ggaactcggg gtcgaagcgc gctacgcgcc
     2461 gctgaacgac atcacgtcgg tgaacggcaa gatcggcggt gctgcccagg cgcggcgcgg
     2521 cggcgcgatc ctccatcaca cgacgatggc gtaccagatc aacggtcccc tgatgtcgca
     2581 gatcctgcgc gtcggcaagg agaagctgag cgacaagggc gtgacttccg ccgacaagcg
     2641 cgtcggtcca ctccgccagc agaccgacct cgagcgaggc gcgatcatcg atcacctgat
     2701 cgctcacttc cggcgtcgct tcggcctcat cgacgatgag ttcacgccgg aggagctcga
     2761 cgagggggcg gaggcggtcc ggaccaggtt cggcacgcgg gaatgggtct acttccttcc
     2821 ataagaatgt ggccgggatc gcctgtcgat atctcggggc ccatcatgcg gagcgcgata
     2881 tcggcggtgg cccggatcac acgatccggg cctccgccta ttcttcttcc cggcgctcgc
     2941 ccgaggatcg tgtatcgggt ccatgcgtgc cgcccggcgc gaagccggcg ggcattgacg
     3001 gtatcgcgaa cggaccgggt tttgctccgc gatcgacgtc cgaagtttca catcccgtgg
     3061 gaactcgcgt tatcctttaa gtgaattccc ggcatactcc cagcgatata caaggatcat
     3121 tcggaacgcg tgctacactg ggtcgcggta ggctagagaa tcctcgtggt gtaggtttcg
     3181 caacggctat gggatcactc cagaagcagg caaaccacaa gatcggggcg gcgtcgatcg
     3241 aggcgtggct cttgtcaggg cgcggctacg gtcgtcgtcc tggcgtgcgt gccgcatggt
     3301 gaggtcggta gcgcggatcg gacggccggg tcgattgatg atggtggcgc tttgcgcgct
     3361 gctcctcgtc ggttgtggat cggccggcgg cgacgatatc gcctggcatc ccgtgcgcga
     3421 ggccgtctcc accgttgtag cgactcccgc accaccgacc gcgaccgacg ccccggccgt
     3481 cgcccaggaa ttcacccatc aactctcggc ggccgaactc cagaaatacc agccggacga
     3541 actcggactc gtgcccgtcc ccatgtacca cgcgttcgtg aaagacgacg agagtaagga
     3601 taccggcggc ggtggcgaca gtctcgacga gtggacgcgc accctttccg agttccggtc
     3661 ggatctccag tggtattacg atcacgactt ctacgtcgtc cccatgcgcg attacgtcga
     3721 gaatgacatg aaggtgccgg ccggcaaaca tccgatggtg ctcaccttcg atgacgcgag
     3781 cacccgccag gcgatgtgga tcaagaacaa gaagggccag atcgtcgccg atccggattc
     3841 cgcactcggg gtgatggagg acttctacgc gaagcatccc gacttcggac gcggcgggta
     3901 cttcgcggtc ctcatcttca actgcttcaa caaccccgac ggtccgcccg agatggccga
     3961 ctgcgcgacg aagctgaact ggctgatcga ccatgggtac gaagtcggca accacacctg
     4021 gggccaccag gatctcaccg atatcaccga tgacgagttc ctctatcagg tcggcaagcc
     4081 gatcctctgg ttccgccaga acatcgagga caagggtgac atgagcgagg tgctgaccct
     4141 gccttatggc gcctatcccg accgcgcgga aacgcagcag tgggattacc tcgtcaacgg
     4201 cttcgactat cacggcgagc gggtcgacct cgagggaatc ctccaggtga atggcggccc
     4261 cgcgctgtcg ccttccagcg acaagttcga tcgctacggg atcgcccggt tcaacaccga
     4321 tcccgatgtc atggcgtact ggtggaagcg gatcgaggac ggcgaactga cgatgtacac
     4381 cagcgacggc aacccgaac
//
LOCUS       DASTMD010000614         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1129520, whole genome shotgun sequence.
ACCESSION   DASTMD010000614 DASTMD010000000
VERSION     DASTMD010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129520"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFQ54_09070"
     CDS             complement(<1..394)
                     /locus_tag="VFQ54_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165185.1"
                     /translation="MSTTTQKRPLTADDLFAIKLVGNPVASPDGKHIAWVVVTVDKEK
                     DGYVSAIWIADADGGNQRKLTAGSARDTSPVWSPDGTQLAFVSNRAPSKPFPKPAKAD
                     KADEEKQGKKPVKPAADRLRNQVWTIAID"
     gene            779..>2280
                     /locus_tag="VFQ54_09075"
     CDS             779..>2280
                     /locus_tag="VFQ54_09075"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU0165186.1"
                     /translation="MAWRNHRADLSRFLVALIYLVAGAGLGTTGALLDNYLHRGVETG
                     AGTPYVVQPTGKELATNLDLRLFPDSEYDNVSQELNQSGFRYVRQVFSWGQIEPNQGD
                     YQWAAYDAIVKSLSTHGVKIIAVIEQTPQWATGGSLVQANDRPPSDPKTMQTFMQALT
                     KHYAADGQQDIPFVQIWDKPNLASQWGGSPGSGASFLPFLAAAYNGALAGDAEVKVIT
                     PELAMTSDDPLGESDLSFLRDLLSAGGGSFADIYGMQLDGGSYSPDDRRVDVSRDNFS
                     RAILYRDLLVDMGEADKPVWATSYGWAAQGSVSRDLQGQYVVRGLNRSWSEWPWMGLM
                     VQWSFIDPDANAPDAKYAVVLPSGRGTPLYTQLTEDAVQQRSKIANTGFAPMDSQSVS
                     YSGNWQDQHLEGRTFRTTSESESTATLTFRGTGLIAYIRTGPQSGDVKIELDGKVVSG
                     GGGSDGTDWTFYDQFGTNDYPHTLLSGLSDTTHTVTITLTGEGELTIGGL"
BASE COUNT          423 a          704 c          725 g          428 t
ORIGIN      
        1 cgtcgatcgc gatcgtccag acctggttgc gcagtcgatc ggcggccggt ttcaccggct
       61 tcttgccttg cttctcctcg tcggccttgt ccgccttcgc cggcttcggg aacggcttcg
      121 aaggtgcacg attggagaca aaggcgagct gcgtcccatc cggtgaccag accggcgacg
      181 tatcccgggc ggagccggcg gtcagcttgc gctggttgcc gccgtcggca tcggcgatcc
      241 agatcgcgga gacatagccg tccttctcct tgtcgactgt cacgaccacc caggcaatgt
      301 gcttgccatc cggggacgcg acaggattcc cgacaagctt gattgcgaag agatcatcgg
      361 ccgtaagcgg tcgtttctga gtggtagtgg acatcctggt ctctagctcc cgtgatagca
      421 acgcgtcgaa gcaccgttcc ggcaggatac ccgcggttcg cgaaacgctc aatgctgcgg
      481 tagttgcgta aaggtcgcct atacttgccg cgctgatgtc gatcgcggca tggtcgccac
      541 tcgtggggga gggtggtttg tcctgcatgc agcgatgtgc acaggcgctt cccgctgatt
      601 tgagacccat cgccagagag acgcctcgga gtcgcagcca tccgtgtcac gccaataccc
      661 gtttgaacca cccgagctcg aacagtttga aggacgtcgc cgcgggagga agtccagcgg
      721 cgagccgagt cttgccgtac gctcggcgag caccgtcgcc ggatggatcc tgcgactcat
      781 ggcgtggcgc aatcaccggg cggatctcag ccgattcctc gtcgccctga tctacctcgt
      841 tgccggcgcg ggcctgggga ccacgggcgc gcttctcgac aactacctgc atcgaggagt
      901 cgagacgggc gccggcactc catacgtcgt gcaaccgacg ggaaaagagc tggcgacgaa
      961 cctcgacctg cgtctcttcc cggattcgga atacgacaac gtctcccagg agctcaacca
     1021 gagcggtttt cgctacgtgc ggcaggtctt ctcctggggg cagatcgagc ccaatcaggg
     1081 cgattaccag tgggcggcct acgacgcgat cgtcaagtcg ctttcgacgc acggtgtcaa
     1141 gatcatcgcg gtgatcgagc aaacgccgca gtgggccacg ggcggaagcc tggtgcaggc
     1201 gaatgaccgc ccgccgtcag atccgaagac catgcagacc ttcatgcagg cattgacgaa
     1261 gcattacgcc gccgatggcc agcaggacat tcccttcgtc cagatctggg acaaaccgaa
     1321 tctcgcttcc cagtggggag gatcgccggg aagcggggct tccttcctgc cgtttcttgc
     1381 cgcagcctac aacggggcgc tcgcggggga tgcggaggtc aaggtcatca cgcctgaact
     1441 cgcgatgacc tccgatgatc ccctcggcga gtccgacctc tcgttcctcc gcgatcttct
     1501 ttccgccggc gggggctcgt tcgccgacat ctacgggatg caactggatg gcggcagcta
     1561 ttcgccagac gatcgtcgcg tcgacgtgag ccgggacaac ttctcgcgtg caattctcta
     1621 ccgcgacctt ctcgtcgata tgggcgaagc ggacaaaccc gtctgggcga cgtcctatgg
     1681 gtgggcagcc cagggaagcg tcagccgcga tcttcagggg cagtacgtcg tccgcggcct
     1741 gaatcggtcc tggtccgagt ggccgtggat ggggctgatg gtgcaatggt ccttcatcga
     1801 tcccgacgcg aatgccccgg acgccaaata cgcggtcgtg ctcccgagtg ggcgtggcac
     1861 gccgctctat acgcagctga ccgaggacgc ggtgcaacag cggtcgaaga tcgcgaacac
     1921 cggttttgcc ccgatggatt cccaatcagt ctcatacagt ggaaactggc aagatcaaca
     1981 tctggaaggg agaaccttcc gaacgacaag cgagtcggag tcgacggcga cgctgacatt
     2041 ccggggaacg gggctgatcg cctatatccg gaccggtccg cagtccggcg atgtgaagat
     2101 cgaactcgat ggaaaggtgg tctccggagg aggggggagt gacggcaccg actggacctt
     2161 ctacgatcag ttcgggacga acgactatcc gcacacactc ctgtccggcc tctcggatac
     2221 gacgcatacc gtgacgatta cgcttaccgg ggagggcgag ttgacgatcg gcggtctgga
//
LOCUS       DASTMD010000615         1634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1844708, whole genome shotgun sequence.
ACCESSION   DASTMD010000615 DASTMD010000000
VERSION     DASTMD010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1844708"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFQ54_09080"
     CDS             complement(<1..494)
                     /locus_tag="VFQ54_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0165187.1"
                     /translation="MIDSPVAAVPDPSTQTPAGAAPVVVAEHLSKHFKLGGELVQAID
                     DIDLSLPRGKMIALRGQSGSGKSTLLGLFGALDSATSGTLTVDGVVVTSLRGSAEVDY
                     RRRKTGFIFQSFNLIPYLTALENVMLPLELVGESKTRQIERARELLKRVGIAADRQLH
                     RPTRL"
     gene            complement(487..>1634)
                     /locus_tag="VFQ54_09085"
     CDS             complement(487..>1634)
                     /locus_tag="VFQ54_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0165188.1"
                     /translation="NDDATAISGLDHVTALVGILSAFQIDSTLTAPAIELPAGVTPPA
                     GFDSSQSTTTTDTSGVRIQTEGTSDPSSIVSASTTGTGAMTLTSGVLFTADQLDADVA
                     VISESLATTNNLAVGSTITIGGTALKVVGIVTTSDSGRGIAIYLPLTTAQKVLDEADT
                     LSEIRLDVDDISNVDAVAAAVTAKLGTDFSVTTSEDQYNQIASTLKDAQNSSRVALVV
                     AGLASCLVIIFGVILSARQRIKEIGIMKAVGAANRQVVLQFGIETLVVGLIAGILGSL
                     VTFPVAQSVANGLTSSGTSSSGGGGFSPGGGGGNGGAGAAFFQAGRGVGQGVQRAGGV
                     LGSINVAVSPVIFLYALAFAVVLALVASIGPAMQVSRVRPAEVLRHD"
BASE COUNT          296 a          535 c          521 g          282 t
ORIGIN      
        1 agccgggtcg gacgatgaag ttgacgatcg gccgcgattc ccacccgctt caggagttcc
       61 cgcgcgcgtt cgatctgtcg cgtcttcgat tcgcccacga gttcgagcgg cagcatcacg
      121 ttctcgagcg cggtcaggta cggaatcagg ttgaagctct ggaagatgaa ccccgtcttg
      181 cgacgacggt agtccacctc cgccgatccc cggagtgacg tcaccaccac gccgtcaacc
      241 gtcagcgtgc cgctcgtcgc ggaatcgagc gcgccaaaca ggcccaggag cgtgctcttc
      301 cccgatccac tctggccacg gagcgcgatc atcttgccgc gcggaaggga aagatcgata
      361 tcatcgatcg cctgcaccag ttcgccgccg agcttgaaat gcttgctgag gtgctcggct
      421 acgaccacgg gcgcggcacc cgccggtgtc tgggtcgaag gatctggcac ggcagctacg
      481 ggagaatcaa tcatggcgca gtacctccgc cggacgaacg cgcgatacct gcatcgccgg
      541 tccgatgctg gcgacaagcg ccaggacgac ggcaaaggca agcgcgtaga ggaagatcac
      601 gggtgatacc gcgacattga tcgagccgag gacaccgccg gcgcgctgca cgccctggcc
      661 aaccccgcgg ccagcctgga agaaggccgc tcccgctcca ccgttcccac cacctccgcc
      721 ggggctgaat ccgccgcccc cgctgctgga cgtaccggag gaggtgagtc cgttggcgac
      781 ggactgcgcg acggggaacg tcacgagaga tccgaggatg ccagcgatca ggcccacgac
      841 aagcgtctcg atcccgaact ggaggacaac ctgccggttc gcggcaccaa cggccttcat
      901 gatgccgatt tccttgatcc gctgtcgcgc ggagagaatg acaccgaaga tgatgacgag
      961 gcaactcgcc agacccgcca cgacgagcgc gacacgactg gagttctggg catccttgag
     1021 cgtgctcgcg atctggttgt actgatcctc gctcgtggtg acgctgaagt cggtcccgag
     1081 ctttgcggtc accgccgcgg caacggcatc gacattcgag atgtcgtcga catcgagccg
     1141 gatctcgctc aaggtgtccg cttcgtccag gactttctgg gcggtcgtca ggggcaggta
     1201 gatcgcgatc ccccgtccgg aatcactcgt cgtcacgatt ccgacgacct tgagggcggt
     1261 gcctccgatc gtgatcgtcg aaccaaccgc cagattgttc gtcgtcgcga ggctctcgct
     1321 gatgacggcg acgtcggcgt cgagctggtc cgcggtgaag agcacgcccg acgtcaacgt
     1381 catcgccccg gtgccggtcg tcgaggcact gacgatcgac gatggatcgc tggtgccttc
     1441 ggtctggatc cgaactccgg atgtgtccgt cgttgtcgtc gactgactgc tatcgaagcc
     1501 ggcgggcggt gttaccccgg ccggcaactc gatcgccggc gccgtcagcg tggaatcgat
     1561 ctggaacgcg ctcagtatcc cgacgagcgc cgtgacgtga tcgaggccgg agatcgctgt
     1621 cgcgtcgtca ttcg
//
LOCUS       DASTMD010000616         2899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1217956, whole genome shotgun sequence.
ACCESSION   DASTMD010000616 DASTMD010000000
VERSION     DASTMD010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217956"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1812)
                     /locus_tag="VFQ54_09090"
     CDS             complement(<1..1812)
                     /locus_tag="VFQ54_09090"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU0165189.1"
                     /translation="MTETIARGIQPVAERALSDDVYLLAGILGNVIKSIAGKEAFDLE
                     EDARNLAKNLRRGELEAGPQLEELVGGADTEELRMLIRAFTNYFQLINLAEDNERIRR
                     VRRREQTDPTSPRRGSIREAVLVLAGHGISAGEVQTMLNEAQVRLVLTAHPTEARRRT
                     VIDKLARIFATIRDLDERHALPHEVSRARTRLSSTIASLWSSNELRLTKPTVLDEVRA
                     VLIYLGSTLSTVIPEIYRDLEEAIAEAYPDSGITIPPFLTFGSWIGGDRDGNPFVTPD
                     VTVEALGVMRAASLGLLEGRLTELAGRLSISDLMVRPVETLQPTLESYQAMFPELADE
                     VAHLNRGEPYRQLLTLMRERLRATRDGGAHAYDSAKGLIADLRLIEDALRTQSAEMIG
                     LGELHDFIRLVEVFGFEFATLDIREHAKRHSTALQAAFAASGVEPDYEALDEDDRVAL
                     LLREIDNPRPLVPLDLDRLPDAAREVISTFRTIRWLMTHGYPDAIQTYIISGTESPSD
                     MLEVLLLMKESRLASPGGEDALLRIAPLFEEGATLRSSQATMTTMLEAPSYARALASS
                     GGIQEIMIGYSDSNKDVGYLASSWELQQAQADLAALLE"
     gene            2085..2651
                     /locus_tag="VFQ54_09095"
     CDS             2085..2651
                     /locus_tag="VFQ54_09095"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU0165190.1"
                     /translation="MDSVVSLRRPTPDDAELIHAWRAQPSIGRFQPTRLLPVEHWRDE
                     LARQAARPLDSSLAAKVQWLIEEDDLPAGWISLDVTSRTHGIAAIGYSVSEEFRGRRL
                     VGRALRQVIAMAFDPGVLALERLEAVCSGLNVPSRRVLAAAGFREEGIARGLLIINGE
                     RVDHIMWGLLRTDLTDSDRNHDNSPSSI"
     gene            2680..>2899
                     /locus_tag="VFQ54_09100"
     CDS             2680..>2899
                     /locus_tag="VFQ54_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165191.1"
                     /translation="MTANEKSPVLGYEEIADRLIPADPQISPDGRNVAFTVSTNNKKK
                     DGKERAIWLSRDRQPATRFTGGEANNQDP"
BASE COUNT          530 a          973 c          897 g          499 t
ORIGIN      
        1 ttccagcagc gccgcgaggt ccgcctgcgc ctgctggagt tcccagctgg aggcgaggta
       61 tccgacatcc ttgttggagt cggaatagcc gatcatgatc tcctggattc ccccgctgct
      121 cgccagtgct cgcgcgtaag agggggcttc cagcatcgtc gtcatcgtcg cctgcgagct
      181 tcgcagcgtc gcgccctcct cgaagagcgg cgcgatccgg agcaacgcat cctcgccacc
      241 cggacttgcg aggcgggact ccttcatgag gagaaggact tcgagcatgt cggagggcga
      301 ctccgttccc gagatgatgt aggtctggat cgcgtcgggg tagccatgcg tcatcagcca
      361 gcgaatcgtg cggaaggtcg agatcacctc gcgcgcggca tccggcagcc gatcgagatc
      421 cagcggcacg agcggccgcg ggttgtcgat ctcccgaagc aacagcgcga cacgatcatc
      481 ctcgtcgagc gcctcatagt ccggttcgac accgcttgcg gcgaacgccg cctgcaacgc
      541 ggtcgaatgc cgcttcgcgt gctcgcggat gtcgagtgtc gcgaactcga aaccgaagac
      601 ctcgacgagc cggatgaaat cgtgcagctc cccaagaccg atcatctcgg cggattgcgt
      661 gcgcagcgca tcctcgatca ggcgaagatc ggcgatcagt cccttcgcgc tgtcgtaggc
      721 atgtgccccg ccatcacgcg tcgcccgcag acgctcgcgc atcagcgtca gcagctgccg
      781 gtagggctcg ccacgattga gatgggcgac ttcgtctgcc aactcgggga acatcgcctg
      841 gtaggactcg agcgtcggtt gcagcgtctc gaccgggcga accatgaggt cggagatcga
      901 aaggcgtccc gccagttcgg tcaggcgccc ttcaagcagc cccagcgagg ccgcccgcat
      961 caccccgagc gcctcaacgg tcacgtccgg cgtcacgaac gggttcccgt cgcgatcgcc
     1021 accgatccag gagccgaagg tcaggaaggg cgggatcgtg atcccggagt cggggtaggc
     1081 ctcggcgatc gcctcttcga gatcgcgata gatctcggga atcaccgtac tcagggtcga
     1141 accgaggtag atcaggaccg cgcggacctc gtcgagcacc gtcggcttcg tcagccgcaa
     1201 ctcgttcgaa ctccacagcg atgcgatcgt cgaggagaga cgagtccgcg cccggctgac
     1261 ttcgtggggc aaggcgtgcc gctcgtcgag gtcgcgaatc gtcgcgaaga tgcgcgccag
     1321 cttgtcgatc acggtccgcc gccgcgcctc ggtggggtgg gcggtgagca cgaggcgaac
     1381 ctgcgcctca ttcagcatcg tctggacttc gccggcactg atgccgtgac ccgcgaggac
     1441 gaggaccgcc tcacgaatcg agccacgccg gggactcgtc ggatccgtct gctcgcgccg
     1501 gcggacgcga cgaatccgct cgttgtcctc ggcgagattg atcaactgga agtagttcgt
     1561 gaaggcccgg atgagcatcc gcagctcttc ggtatccgcg ccgccaacga gctcttcgag
     1621 ctgcggtcct gcctcgagtt caccacggcg aagattcttg gcgagattcc gggcgtcttc
     1681 ctcgagatcg aatgcctctt tccccgcgat cgacttgatg acattgccga ggatgcccgc
     1741 caacagatag acatcgtcgg acagcgcacg ttccgctacc ggctggattc ctcgggcgat
     1801 cgtttctgtc atgcggattc ggtgctccca aatgcgaatg tggcacggcg tcgaagcaac
     1861 tcgagcctgg gcgtgcgcgg cgttcgcccc cttgtctaga atgaaaacag tgccataaac
     1921 gccttccttt tacacgtcat ccgggcctca tgcgtgacca gaacgggtga gcctcttgtg
     1981 cgaacggaac aaacccgacg acccgatgcc gacacgtggc accaaccagc cgcgttggat
     2041 gtcacacgag cacaagtcct gaaccgatag ggatgattat gacaatggat tccgtcgtat
     2101 ccctccgccg tccgactcca gacgatgccg aattgattca cgcctggcgc gcccagccat
     2161 cgatcggccg tttccagcca acccgcctgc tccccgtcga gcactggcga gacgagctcg
     2221 cacgacaggc ggcacgcccg ctcgactcgt cgctcgccgc caaggtgcag tggctgatcg
     2281 aggaagatga ccttcccgcc ggatggatct cgctcgacgt cacctcccgg acgcacggga
     2341 tcgccgcgat cggttactcc gtcagcgagg agttccgcgg ccgccggctg gttggtcgcg
     2401 ccttgcgtca agtgatcgcg atggccttcg atcccggcgt cctcgccctg gagcgcctgg
     2461 aggccgtctg cagcggactg aacgtcccat cccggcgcgt cctcgccgcc gctggtttcc
     2521 gcgaggaagg catcgcccgc ggcctgctca tcatcaacgg cgaacgcgtc gatcacatca
     2581 tgtggggatt gcttcgcacc gacctgaccg attccgaccg gaaccacgac aatagcccaa
     2641 gctcgatctg acgttaccct gaggaggatc acctacttca tgaccgcaaa cgagaagtcg
     2701 ccggtgctcg gctacgagga aatcgccgat cgcctgatcc cggccgaccc gcagatctcc
     2761 cccgatggcc gcaacgtcgc gttcaccgtg tcgaccaaca acaagaagaa ggacggcaag
     2821 gagcgcgcga tctggctctc gcgggaccgt cagccggcga cacgcttcac cggcggcgag
     2881 gccaacaatc aggacccgc
//
LOCUS       DASTMD010000617         3640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_594579, whole genome shotgun sequence.
ACCESSION   DASTMD010000617 DASTMD010000000
VERSION     DASTMD010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594579"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1282)
                     /locus_tag="VFQ54_09105"
     CDS             complement(179..1282)
                     /locus_tag="VFQ54_09105"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0165192.1"
                     /translation="MSTTTTVAAIAVHPGQAGTLRQTRARLPELGPTDVLVDTIRVGV
                     CGTDREIIRGDLGEAPKGTEELVIGHEVVGRVAAIGSAVTAFAPGQIVTATVRRPDDC
                     PACQAGEPDMCLWLKYTERGIFREPGFMAERFVEDQHWLIPVPDRLVSTAVLIEPLTV
                     VEKAVRQAELIQRRLKYWNLQKAMVIGAGPIGLLGTLLLRSRGADVTTLARTPAPNPA
                     ANIVTASGARYVSTQNTTLDEIAAGIGNIDVIIESSGNAEIAMNALRVLGNNGVEVLL
                     SITGGTHSIALPADQMNASLVLGNKTVVGSVNAGAIDFVNAVTSLDRFEELWPGLTER
                     FITNRLPFGPDLDLDRIAHKSPNEIKTVIEFRD"
     gene            complement(1481..1732)
                     /locus_tag="VFQ54_09110"
     CDS             complement(1481..1732)
                     /locus_tag="VFQ54_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165193.1"
                     /translation="MSYAIDGVATRLLGLVAATSALGLAALGAVLFLNADEAQASAML
                     ANNPGSLDPLIEPAQQLLEAFAVSNLNTHKCSSPPSARS"
     gene            1875..2729
                     /locus_tag="VFQ54_09115"
     CDS             1875..2729
                     /locus_tag="VFQ54_09115"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEU0165194.1"
                     /translation="MKVLISADMEGTCGVVSWVQVMPPEVGASPSNQSEYDRARRRMT
                     LEVNAAIEGALAGGADEVIVNDSHDGMRNLLPDELHPAARYISGSDKFYGMMQGITTE
                     GIGAVFYTGYHAKAGTPAAPLAHTWSGYVQDVRFNGLSTGEFGLNAAIAGYFGVPVTF
                     VCGDDKAVAQVEALLGDQVVGVAVKEGLSTFSANHLSPEKARELIRAGAEKAIANVEF
                     ATPYIMPDGVRVELELDHQARIDQTLLMPGTERAGERTVAFYPKSGFEFVQMFRGLMK
                     LSSITMSP"
     gene            complement(2774..3532)
                     /locus_tag="VFQ54_09120"
     CDS             complement(2774..3532)
                     /locus_tag="VFQ54_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165195.1"
                     /translation="MNDALTHIARGLVDRHTFGAVMLVAIAAIGWRNRSSFRGMLADD
                     DAFWRLTARLFVGSFIATAIWIAIFDDWIQLFSEPYRRTRDLSVDRVVSDPIPTELRA
                     ITLTLLVITVVSTATIVARHVGGYGLQIGALLLSVTFWLILFIFHQRLDILVHDGAAD
                     PVEHASDFAGLTMFWVLRTGLGVIVAFLSLGIAVLVVAPFVTLVLDILRIRRPRVAVA
                     ASDEFFATLHRQAQNYVPDDTPQTRKRRSLRRPS"
BASE COUNT          651 a         1086 c         1260 g          643 t
ORIGIN      
        1 caaccggtgg agatgaacct ccagccgtat cgcgtcgagc ggggattgac gacgatctga
       61 gcccgggccg ccagcggtcg tagtgggtca catccttcga caagctcggc gccgctgcca
      121 ggactggctc gttggtggcc agaaacgggg ttgtgccgag cgtttgtcag ccggtgggtc
      181 agtcccggaa ctcgatgacg gtcttgatct cgttcggcga cttgtgggcg atccggtcga
      241 gatcgaggtc gggaccgaac ggcagccggt tcgtgatgaa gcgctcggtc agacccggcc
      301 agagttcctc gaagcgatcg agactcgtca cggcgttgac gaaatcgatc gcgcccgcgt
      361 tgacgctgcc gacgaccgtc ttgttgccga gcacgagcga cgcgttcatc tgatcggccg
      421 gcaaggcgat cgaatgcgtg ccgccggtga tgctgagcag gacttcgacg ccgttgttgc
      481 cgaggacacg aagcgcgttc atcgcgatct cggcattccc cgaactctcg atgatgacat
      541 cgatgttccc gattccggcg gcgatctcgt cgagcgtcgt gttctgcgtc gagacatagc
      601 gtgcgccgct cgcggtcacg atgttcgcgg ccggattcgg ggccggggtg cgcgccagcg
      661 tcgtcacgtc cgctccgcgc gatcgtagca gcagcgtgcc gaggagcccg atcgggcccg
      721 cgccgatcac catcgccttc tgcaggttcc agtacttgag tcgtcgttgg atcagctccg
      781 cctgccggac ggccttctcg acgacggtca gcggctcgat caggacggcg gtggagacaa
      841 gccgatccgg cacgggaatc agccaatgct gatcctcgac gaaccgctcc gccatgaagc
      901 ctggttcgcg gaagatgccg cgctccgtgt atttcaacca gaggcacatg tccggctcgc
      961 cagcctggca ggcggggcaa tcgtccggac gccgcacggt cgcggtcacg atctggcccg
     1021 gcgcgaaggc ggtgacggcg ctgccgatcg cggcgacgcg ccccaccacc tcatgcccga
     1081 tcaccaactc ctcggtgcct ttcggcgcct cgccaagatc gccgcgaatg atctcgcgat
     1141 cggtgccgca gacgccgacc cggatcgtgt cgaccaggac atcggtcggg ccaagctccg
     1201 ggaggcgggc gcgagtctgg cgaagcgtgc cggcctgacc gggatggacg gcgatggcgg
     1261 caacggtcgt ggtcgtggac atgagggcga ctctccaggg gcgatgcatt ggcagtcatc
     1321 gggttggcga cggttcgaat ccggatattg atgccgatgc gagcgtgcgg cgtcaagcgg
     1381 ttgggacccg agtcgttccg tggggtatac tggtgggagc ggatgacatt ccgtggtctt
     1441 tgcgtgtcta tcgggcaccg agaagattct tccagatgca ttaactacgc gctgaaggtg
     1501 gtgatgagca tttatgcgtg ttgagattcg agacagcgaa ggcttcgagc agctgctgcg
     1561 ccggttcaat aaggggatcg agcgatccgg gattattcgc gagcatcgcc gaggcctgcg
     1621 cttcatcagc gttcaggaag agaaccgcgc caagcgccgc aaggccgagc gccgacgtcg
     1681 ccgcaaccag accaagtaat ctggtggcga cgccatcgat cgcataactc acccgaaacg
     1741 cccccgacca cattggtcgg gggcgttttg tgcgttggag ccatcgtcag attgtttcgg
     1801 gtcggatacg gttcgcgcca caacgtcaat gaatcacgtt gctcgtcgat gtcacgtcaa
     1861 ggagctgcgg ttgcatgaag gtcctgatct cagccgatat ggaaggaact tgcggagtcg
     1921 tctcctgggt tcaggtgatg ccgcccgagg tgggagcgtc gccctccaat caatccgaat
     1981 atgatcgcgc ccggcggcgg atgacgctcg aggtcaacgc cgcgatcgag ggcgcgctgg
     2041 ccggtggcgc cgacgaggtg atcgtcaacg atagccacga cgggatgagg aatctgctcc
     2101 cggacgagtt gcatccggcg gcgcgctaca tctccgggag cgacaagttc tacggcatga
     2161 tgcaggggat caccaccgag ggaattggcg cggttttcta tacgggttac cacgccaagg
     2221 cgggaacccc ggccgcgccg ctcgcccaca cctggtccgg ctacgtgcag gatgtccgct
     2281 tcaacggtct ctcgacgggc gagttcggac tgaacgccgc catcgccggg tacttcgggg
     2341 ttccggtgac gttcgtctgt ggtgacgaca aggcggttgc ccaggtcgag gcgctgctcg
     2401 gtgaccaggt cgtcggtgtc gcggtcaagg aggggctctc gaccttctcc gcgaaccacc
     2461 tgagcccgga gaaggctcgc gaactgatcc gggcgggggc agagaaggcg atcgcgaatg
     2521 tcgaattcgc gacgccgtac atcatgcccg atggagttcg cgtcgagctt gagctcgatc
     2581 atcaagcgcg gatcgatcag acgctgttga tgccgggtac cgagcgggcc ggcgagcgga
     2641 cggtcgcgtt ctacccgaag agcggcttcg agttcgtgca gatgttccgc gggttgatga
     2701 agctctccag catcacgatg tcgccgtagt cgtttgtcgt ttgatttccg gcggtccctt
     2761 catcgatcac ccgttacgac gggcggcgga gggaccgccg ttttcgtgtc tgcggcgtgt
     2821 cgtcgggaac gtagttctgc gcttgccggt ggagcgtggc gaagaactcg tccgaggcgg
     2881 ccacggcaac ccgcggtctg cgaatgcgga ggatgtccag gacgagcgtg acgaacggcg
     2941 cgacgacgag cacggcgatg ccgaggctga ggaatgccac gatcacgccg aggcccgttc
     3001 gcagcaccca gaacatcgtc aacccggcga agtcggatgc gtgttcgacc gggtcggcgg
     3061 cgccgtcgtg cacgaggatg tcgagacgct ggtggaagat gaagaggatg agccagaagg
     3121 tgacgctgag cagaagcgcg ccgatctgga gaccgtatcc cccgacgtgt cgagcgacga
     3181 tcgtcgccgt cgagacgacg gtgatgacca gcagggtcag cgtgattgcg cggagttcgg
     3241 tcgggatcgg atcggatacc acacgatcga ccgagagatc gcgggtgcga cggtagggct
     3301 cggaaaagag ctggatccag tcgtcgaaga tcgcgatcca tatcgccgtc gcaatgaacg
     3361 agccgacgaa gagccgcgcc gtgagtcgcc agaaggcgtc atcgtcggcg agcatgcccc
     3421 ggaaggacga ccgattccgc cagccaatcg ccgcgatcgc aacgagcatg accgcgccga
     3481 acgtgtggcg atcgacgagg ccgcgggcga tgtgcgtcag ggcgtcattc atcgagcgcc
     3541 gcgatctcgg catcgacatc ggccaggcgc ttctgggcga gcggcaggcg gtcgcgcagg
     3601 cgatgtcgct cttccgcctc ccgggcgcgc tcatcgagga
//
LOCUS       DASTMD010000618         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2113884, whole genome shotgun sequence.
ACCESSION   DASTMD010000618 DASTMD010000000
VERSION     DASTMD010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113884"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1552)
                     /gene="phnD"
                     /locus_tag="VFQ54_09125"
     CDS             complement(167..1552)
                     /gene="phnD"
                     /locus_tag="VFQ54_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0165196.1"
                     /translation="MEDSGRPQDPEHHGQHGNGVSREAEGGGAPSGSIRPFIFAFAST
                     VAGRTVDHDNRTQPLRNYLERVLERPVRLVAPESYAETLADLQAGRADAAMLGEYATR
                     QGQMIGGVEPLVAPVGADEEVPTYRSAIVTRIDSGIRDLDGLRGKSFGLVDEQSTSGY
                     LVPRAMLREVDIDPDTDVEIRLFGQHRNVIEAVIAGEVVAGAAHEIRLKPPSLDRGPD
                     YARLRVIARSRPIPLGPLVIRSSLDAKTREILAAAMLRVHEVDPVAAEVLIRSGHRFT
                     IASRPASPTLKSIAALAGVSYATVSRVVNESGYVAPATAARVKAVIAEVGYVPNGNAR
                     VLQGQQAPLVGLVIPFGESGEAEDLGARLAAVGVPMVLCPVRGRLAASPFLQVVRDRR
                     LGALIVGPQHIDDPDLMELARTGHAIVAIGVDEDAVAPGVVVTTLDRAAESVLISLGW
                     KGHPALASATR"
     gene            1792..2214
                     /gene="phnG"
                     /locus_tag="VFQ54_09130"
     CDS             1792..2214
                     /gene="phnG"
                     /locus_tag="VFQ54_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HEU0165197.1"
                     /translation="MERTRRCDLLARLPDKDLQALAALVIDAKKDQVVPVTPPTTGMI
                     MARIREGARGDVFNLGEVLVTECQVRVGKREGWSMLMGNRPKGALAAASIDAVTAAGH
                     PATSELDALLSVMIADQDALAAAERAKLATTRVQFDTQ"
     gene            2297..>2484
                     /locus_tag="VFQ54_09135"
     CDS             2297..>2484
                     /locus_tag="VFQ54_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HEU0165198.1"
                     /translation="MALRAELPSTTWDDPIRATQHVFRRTLDALAMPGSIQQLVVNPT
                     LADLDASEEIAWPATLLL"
BASE COUNT          440 a          832 c          822 g          390 t
ORIGIN      
        1 cgaggcgacc acgtgggtcg tcgggacggg aacgcgggcg ccttgtttcc acagacgccg
       61 cggatgggaa gacccggggc gaccacgtgg gtcgccggga cgggaatgcg ggcgcgacgt
      121 gggaacggga tgacgtggag aaggtgacaa tgtatcgcgc gaagagctac ctcgtggcgg
      181 acgcgagcgc cgggtggccc ttccagccca gcgagatcag caccgattcc gccgcccggt
      241 cgagcgtcgt cacgaccaca cccggcgcga ccgcatcctc atcgacgccg atcgcgacga
      301 tcgcgtgccc ggttcgggcg agttccatca gatccggatc gtcgatgtgc tgggggccga
      361 cgatcaacgc ccccagccgc cggtcgcgca cgacctggag gaatgggctc gccgcgaggc
      421 gtccgcgcac cgggcagagc accatcggca cgccgaccgc ggccaggcgc gcaccgagat
      481 cttccgcttc ccccgattca ccgaacggaa tcaccagccc gacgagcgga gcctgctgcc
      541 cctggagcac gcgcgcattg ccgttgggga cgtagccaac ctcggcgatc accgccttca
      601 cccgcgccgc cgtcgctgga gcgacatagc cggattcgtt gacgacgcgg gagaccgtcg
      661 cataggagac cccggcgagg gcggcgatgc tcttcagcgt cggcgatgcg ggtcgcgacg
      721 cgatcgtgaa gcggtgaccg ctccggatca gcacctccgc cgcgacagga tccacctcgt
      781 ggacgcggag catcgctgcc gccaggatct cccgggtctt cgcgtcgagc gagctgcgaa
      841 tcacgagcgg accgagcgga atcgggcgcg atcgcgcgat cacccgcagg cgtgcgtagt
      901 cgggtccgcg atcgagcgac ggcggcttga ggcggatctc gtgcgcggcg ccggcgacga
      961 cctcacccgc gatcaccgcc tcgatcacgt tgcgatgctg gccgaagaga cggatctcga
     1021 catcggtgtc gggatcgatg tcgacctcgc gcagcatcgc gcgcggcacg agatatcccg
     1081 atgtcgactg ctcgtcgacg agaccgaaac tcttcccgcg gagaccatca agatcgcgga
     1141 ttccggaatc gatgcgcgtg acgatcgcgc tgcggtaggt cgggacctcc tcatcggcgc
     1201 cgaccggcgc gacgagcggt tcgacgccgc cgatcatctg cccctgacgg gtggcgtact
     1261 cgccgagcat cgccgcgtca gcgcgccctg cctggagatc ggccagcgtc tcggcataac
     1321 tctcgggtgc gacgagacga accggtcgtt cgaggacccg ctcaaggtaa ttgcggagcg
     1381 gctgcgtccg gttgtcgtga tcgaccgtgc gcccggcgac ggtcgaggcg aaggcgaaga
     1441 tgaagggacg gatgctgccc gatggcgcac cgcccccttc ggcttcgcgg gatacgccat
     1501 tgccatgctg gccgtgatgt tcgggatcct gtggacgacc cgaatcttcc attaaaaccc
     1561 ctcgcgtcag atgtcgcttc ctcgcgactg tatgccgagc atatccggag tggcggccgt
     1621 tgtcgcggcg aatcttctct cgacatcatc acatgggcat ttctgaaaag ctttacagat
     1681 cccttaacca tcgcgtgtgt gtgacgacgt atcttccgag aagacgaatt gagggcgtca
     1741 ggccacaacg catgtcatta cggatatgca tggaagaaga ttggagcccc gatggagcga
     1801 actcgccgct gcgacttgct tgcccgcctg ccagacaagg atctgcaggc acttgccgcg
     1861 ctcgtgatcg acgcgaagaa ggatcaggtc gtgccggtga cgccaccgac gaccgggatg
     1921 atcatggcgc gcatccgcga aggcgcccgc ggtgatgtct tcaacctcgg cgaagttctc
     1981 gtcacggagt gccaggtccg ggtcgggaag cgcgagggct ggagcatgtt gatggggaac
     2041 cgccccaagg gcgcgctcgc cgctgcctcg atcgatgccg tgactgccgc cggtcatccg
     2101 gcgacatccg aactcgacgc cctgctttcc gtgatgatcg ccgaccagga tgcgctcgcc
     2161 gccgccgagc gagccaagct cgcgacgacg cgggtccagt tcgacaccca gtaggccgtc
     2221 ctgggcacgg gaaggcggga catacacggt cccgccccac gcaaccccaa tgattgaccc
     2281 tgaccaaagg attcgcatgg ctctccgcgc agaacttcca tctacgacct gggacgaccc
     2341 gatccgggcc actcaacatg tgttccgccg cacgctcgat gccctcgcga tgccggggtc
     2401 gatccagcag ctcgtggtca acccgacact cgccgatctc gatgccagcg aggagatcgc
     2461 ctggccggcg acgctgctcc tgat
//
LOCUS       DASTMD010000619         3974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2916354, whole genome shotgun sequence.
ACCESSION   DASTMD010000619 DASTMD010000000
VERSION     DASTMD010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2916354"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /gene="mreD"
                     /locus_tag="VFQ54_09140"
     CDS             complement(<1..282)
                     /gene="mreD"
                     /locus_tag="VFQ54_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU0165199.1"
                     /translation="MNRTLWSTSLSPMSRIIFGLLLFVTIFAQATLIPAWNPLRFGPD
                     LVVVLLFLWSGISGPREALFWVFVSGLLLDILSADPLGTNALSLVIVVIV"
     gene            566..1399
                     /locus_tag="VFQ54_09145"
     CDS             566..1399
                     /locus_tag="VFQ54_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU0165200.1"
                     /translation="MTSSSSPVSPISPVSPLVTVDWLAAHLSDSSPLLVIDVRPQPSY
                     LAGHIPGAISLDVAALRLPTSDDQTIEAWTAFLQKGLRGAGIRGDQQVVFYEDVTGTL
                     SSFGVWLLDVAGLHNGALLDGGIRAWHAAGQPLSTERVTATPSDVTLDLDRSALATAT
                     EIISDLGGDTHSFQMVDTRGIAENSAGTIPTSVNVEWVNTLGPTGALKSAEELKTLYA
                     SAGLDPDQPAVTYCAGGFRAAHTYVVLKSLGFQDVRSYAPSWSEWSQLPETPIEYPSG
                     S"
     gene            1578..2891
                     /locus_tag="VFQ54_09150"
     CDS             1578..2891
                     /locus_tag="VFQ54_09150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEU0165201.1"
                     /translation="MTLLGPTDSLAPITPKRVVITGVGAISSLAPSAEETWKRILANE
                     TGIRRVDSLDPAQHPCVLRGDVNDDDIPSRFLKGKEARNTSRFSRMAIEAAGDAMIDA
                     GLLDDNLQPIIDLTAAGAALGTCLGGTYDDVLPVFQTAITRETYSRVPPHLHVMFPHN
                     MAAYTIQKQFGMQGPSSTVVTACATGSQAIGDGFHAIKYGHAPLMIAGSAESTHHPLF
                     QAGFAAMRALVTDSNDNPDGASRPFDATRAGFVLGEGSGMLVLEELEHALARNATIYA
                     EIAGYATSNDAYHPIAPQPDGIGAVRAIEQALKDANIDASEVDHINAHAASTPAGDLA
                     EAKAIAHVFGDRASSIPVTSVKGAIGHCMATAGALETITAVLTIRDGLIPPTRNLNNP
                     DPEIGLDIVHGTPREHRVNVMTKHSFGLGGQNACLVLRRYDGVAG"
     gene            2875..3282
                     /locus_tag="VFQ54_09155"
     CDS             2875..3282
                     /locus_tag="VFQ54_09155"
                     /inference="COORDINATES: protein motif:HMM:NF022279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrdB family protein"
                     /protein_id="HEU0165202.1"
                     /translation="MASPDNAASAPPHQPLTIGVVRFALEIFAIGGLGWWGWRLGNGG
                     VPGTIVAIAIPVIAFTIWGVFAVKDDPSRNPNPPVPVAGWIRVIIEFAVFGLAAYGMW
                     TNGGRAWAETLLTGVGITYFVSYDRIVWLLKQR"
     gene            3307..>3974
                     /locus_tag="VFQ54_09160"
     CDS             3307..>3974
                     /locus_tag="VFQ54_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165203.1"
                     /translation="MYVRQAKDRAKSWVDAHASATPGFAGAYLAGSILDRDDAVALPS
                     ASDVDLMVVVAGDPTTKSGKIVVDGVILEITLLPRDQFQSAVAILPDYHLAHSFAKTT
                     LLADPTGALTPLQRDVANAFGDPEWIRRRCADARSRVVGRLSTLSPDAPFHQQVVQWM
                     FGNGVVTHVLLVAGLQNPTVRTRYVAVRDLLARTGHAGIYEPLLALIGCRDIDARQLD
                     TRLDA"
BASE COUNT          697 a         1369 c         1221 g          687 t
ORIGIN      
        1 cacgatcacg acgatcacga gcgacagggc gttggtgccg agcgggtcgg cgctgaggat
       61 atcgagcaga agaccactga cgaagaccca gaagagcgcc tcccgcggtc cgctgatgcc
      121 ggaccagagg aaaagaagaa cgaccaccag gtcgggtccg aacctgaggg gattccaggc
      181 agggatcaac gtcgcctgcg caaagatcgt gacgaagagc agaagcccga agatgattcg
      241 cgacatgggt gagagtgatg tgctccatag ggttctgttc aaccgggttc cgggagagaa
      301 cgccatcgga cgcatggctc accaacacct tggtgccggc cccggcatct ttgtcgatgc
      361 ggtcgaaccg gcggcctgca tctgaggcca acacgccgaa tccgcccggg gcaagcgtcg
      421 tcgcccgagt gtaccacgca cctgtatgga cactatgagc gaccgcatcg tccgatggga
      481 ggcgacgcga caagggggaa cgcgatgcgc gatcatgttc ccagacgtcc tctttcgttc
      541 gcgaatcctg gaacaggagc aacacgtgac ctcgtcctcc tcacccgttt ctccgatctc
      601 gcccgtctca cccctcgtga cggtcgattg gctcgccgcc cacctctcgg actcctcccc
      661 attgctcgtg atcgacgtgc gaccgcaacc gtcctacctc gccggtcata tcccgggcgc
      721 gatctcgctc gatgtcgcgg ccctccgcct cccgacctcc gacgatcaaa cgatcgaggc
      781 gtggacggcg tttctgcaga agggactccg cggcgccggc attcgcggtg accagcaggt
      841 tgtcttctat gaagacgtca ccggcacgct gtcctccttc ggagtctggt tgctcgatgt
      901 cgcgggcctc cacaacgggg ccctgctcga cggcggcatc cgcgcctggc atgcggccgg
      961 tcagccgctc tccaccgaac gcgtcaccgc gacgccgtcc gacgtgacgc tcgacctcga
     1021 tcgttcggcc ctcgccacgg cgaccgagat catctcggac ctcggcggcg atacccattc
     1081 gttccagatg gtcgataccc gcggcattgc cgagaacagc gccgggacga ttccgacctc
     1141 ggtcaatgtc gagtgggtca atacgctcgg accaaccggc gccctcaaga gcgccgaaga
     1201 actgaagacg ctctacgcat ccgctggtct cgatccggat cagcctgccg tcacctattg
     1261 cgcgggcggc ttccgggctg cccataccta cgtcgtgctc aagtccctgg gtttccagga
     1321 cgtccgctcc tacgccccgt cgtggtcgga atggtcgcag cttccggaga ccccgatcga
     1381 gtacccgtcc ggctcctgac gccgatccca ccgtcgcgaa cagctgtcgt gcgccgcacc
     1441 gggcggtcgc atcgggcgac agccgttcgc gcgcgtgccg tatgatcgtt cgcacagaaa
     1501 gatgattgga ttcaggcatc cagtccggaa ctaccgcttc cgcgtctgga tgacgcgggg
     1561 cgcgaaaggc tcagtacgtg acattgctcg gaccaaccga ttcccttgcc cccatcaccc
     1621 cgaagcgggt cgtgatcacc ggtgtcggcg cgatcagttc gctcgctccc tccgccgagg
     1681 agacgtggaa acggatcctc gccaacgaga cgggaatccg ccgcgtcgat tccctcgatc
     1741 ccgcccagca tccctgcgtc ctccgtggcg acgtcaacga tgacgacatc cccagccgct
     1801 ttcttaaagg aaaggaagcg cgcaacacct cacgcttctc gcggatggcg atcgaggccg
     1861 ccggcgacgc gatgatcgac gccggtctcc ttgacgataa cctgcagccg atcatcgatc
     1921 tcaccgcggc gggggcggcg ctcggcacct gcctgggcgg cacctacgac gatgtgctgc
     1981 ctgtcttcca gactgccatc acacgcgaga cctattcacg cgtcccaccc cacttgcatg
     2041 tgatgttccc gcacaacatg gcggcctata cgatccagaa acagttcggg atgcagggac
     2101 cgagctcgac cgtcgtcacc gcatgtgcga ccggatcgca ggcgatcggg gatggcttcc
     2161 acgcgatcaa gtacggtcac gcgccgctga tgatcgccgg ttccgccgaa tcgacgcacc
     2221 acccgctctt ccaggcggga ttcgcggcga tgcgggccct cgtgaccgac tcgaatgaca
     2281 acccggacgg cgcgagtcgt ccattcgacg cgacccgcgc cggtttcgtg ctgggcgagg
     2341 gatcgggcat gctcgtcctc gaggaactgg agcatgcgct cgcgcgcaac gcgacgatct
     2401 acgccgagat cgcgggctat gcgacctcga acgacgcgta ccatccaatc gccccgcagc
     2461 cggacgggat cggcgcggtg cgcgcgatcg agcaggcact caaggatgcg aatatcgacg
     2521 cgtccgaggt cgatcacatc aatgcccatg cggcctcgac gccggcgggc gacctcgccg
     2581 aggcgaaggc gatcgcgcac gtcttcggcg accgcgcgtc gtcgatcccg gtgacgtcgg
     2641 tcaagggagc gatcggccac tgcatggcga cggccggcgc cctggagacg atcacggctg
     2701 ttctcaccat ccgcgacggc ctgatcccac ccacccgtaa cctgaacaat ccggatcccg
     2761 agatcggcct cgacatcgtc catggcacgc cacgcgagca ccgcgtgaac gtaatgacca
     2821 agcattcttt cggcctcggc ggtcagaatg cctgcctcgt gctccgacgc tacgatggcg
     2881 tcgccggata acgcggcgtc cgcgcctccc caccagccgc tcacgatcgg cgtcgtccgc
     2941 ttcgcgctgg agatcttcgc gatcggcggc cttggctggt ggggatggcg tctcggcaac
     3001 ggcggcgttc ccggaacgat cgtcgcgatc gcgattccgg taatcgcctt cacgatctgg
     3061 ggcgtcttcg ccgtcaagga cgatccatcg cgcaatccga acccgcccgt cccggttgcg
     3121 ggctggatcc gggtcatcat cgaattcgcc gtcttcggac tcgccgccta cgggatgtgg
     3181 acgaatggcg gtcgcgcctg ggcggagacg ctgctgaccg gcgtcgggat cacctacttc
     3241 gtgagctacg atcggatcgt ctggctcctc aagcagcggt aggcgatccg ccccgggaga
     3301 caccgaatgt atgtccgcca ggcgaaggac cgggcgaaat cctgggtcga cgcgcatgcg
     3361 tccgccacac cgggattcgc cggcgcgtac ctcgccggtt cgattctcga ccgcgatgat
     3421 gcagtggcgc tgccatccgc atccgatgtc gatctgatgg tggtcgttgc gggagatccg
     3481 acgaccaagt caggcaagat cgtcgtcgat ggcgtgatcc tcgagatcac gctgcttccg
     3541 cgcgatcagt tccagtcggc ggttgcgatc ctcccggact accacctcgc ccacagcttc
     3601 gcgaagacaa cgctcctcgc cgatccgacc ggagcactga cgccgcttca gcgagacgtc
     3661 gcgaacgcct tcggcgaccc ggaatggatc cggcgccgct gcgcagatgc ccgttcgcgg
     3721 gtggttggac ggctctcgac gctctctccc gacgcaccat ttcatcagca ggtcgtgcag
     3781 tggatgttcg gcaacggtgt ggtgacgcac gtcctgctcg tcgccggtct ccagaatccg
     3841 acggtgcgga cacgttatgt ggcggtgcgc gatctgctcg cacgaaccgg tcatgccggg
     3901 atctacgagc cgttgctcgc actgatcggc tgccgcgata tcgacgcacg gcagctcgac
     3961 acccggcttg atgc
//
LOCUS       DASTMD010000620         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_862619, whole genome shotgun sequence.
ACCESSION   DASTMD010000620 DASTMD010000000
VERSION     DASTMD010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862619"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1720)
                     /locus_tag="VFQ54_09165"
     CDS             complement(<1..1720)
                     /locus_tag="VFQ54_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165204.1"
                     /translation="MPQATDIVLDGAGYMIVPATGANATYTRAQDGMAEGRTARIQIR
                     DFFGGQHRAFQLERDRGGDSLAIGPALGGQGVVPWPLTATATIASGVDLPTTATPFPA
                     ATIGNHVFFARGRYLYRTVALDATTWAPETQVWDAGAGQAITDVASYNGDILIALGTS
                     EDIQVLPYPGGGVSSTLLAGEQAQHVLGYGGFAIYSRADAGSPVGASNQIRMVTGSGI
                     DIRYLDADVLKLTTVGGEAFAATKIALYSFAGKVQETRVTVIVDPGPPPVTEQRDVLL
                     WNGDWDAYFQHGNWTEDDDYRVVVGYGGRLYVWLNKTVLEHNPSGDRAGWRDTGLGGL
                     RCLGGCVVGGNLVIALETADHRSEVWAWNGAGWWRIASRPAASGIWCWPVALSGAGNW
                     DLMLFTEGSTTIDLVRMIWRAADKHTLPSAAQYVTSMLDAGERDKNKAWRKIGAVFAS
                     PDVPPVTTSTDPVTVALDYSVDAGVTWVQAATESLAGSSLANANITLDAELALDGPVS
                     RWLQLRVRWSSVVDWAPVLAGVWAEFEVLDSPARRRRWQMTVIARDQTVDRSGSPLAR
                     TGRELIS"
     gene            complement(1821..>2194)
                     /locus_tag="VFQ54_09170"
     CDS             complement(1821..>2194)
                     /locus_tag="VFQ54_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165205.1"
                     /translation="VRDAWNDTVDYVRDRIPDLLEPVIEFVEEVVGAWLWVLEKVAIV
                     YPVIFERWETAADERVCPECGPIYGAIWEEGSGPHPPVHVNCRCRRVLAWTEWRTRYV
                     EDWRLRWTTWTEWETRITGWA"
BASE COUNT          353 a          747 c          737 g          357 t
ORIGIN      
        1 cggagatcag ctcgcgaccg gtgcgggcga gcggtgaccc gctgcgatcg accgtctgat
       61 cgcgggcgat gaccgtcatc tgccagcgtc gcctccgcgc cggggagtcg agcacctcga
      121 actcggccca gacaccggcc agcaccggcg cccagtccac gacactcgac cagcgcacgc
      181 ggagttgcag ccagcgggaa accggtccat ccagtgcgag ctctgcatcg agcgtgatgt
      241 tggcgttcgc cagcgagctt cccgcgagcg attccgtcgc tgcctggacc caggtgacgc
      301 cggcatcgac ggagtagtcg agcgcgacgg tgacgggatc cgtgctcgtc gtgaccgggg
      361 gaacgtccgg cgaggcgaag accgcgccga tcttgcgcca ggccttgttc ttgtcccgct
      421 cgccggcatc gagcatcgac gtcacatact gcgcggctga tggaagcgtg tgcttgtcgg
      481 cggcgcgcca gatcatgcgc acgagatcga tcgtcgtgct gccctcggtg aagagcatca
      541 ggtcccagtt ccctgcgccg gagagcgcga ccggccagca ccagataccg gacgcggccg
      601 ggcgggaagc gatgcgccac cagccggcgc cgttccaggc ccagacttcc gaccgatggt
      661 cggcggtttc gagtgcgatc acgagattgc cgccgacgac acatcctccg agacagcgga
      721 ggccgccgag tccggtgtcg cgccacccgg cgcgatcgcc ggacgggttg tgttccagca
      781 ccgtcttgtt cagccagacg tagagtcgcc cgccgtaacc gacgacaacc cggtagtcat
      841 cgtcttcagt ccagtttcca tgctggaaat aggcgtccca gtcgccgttc cagagcagga
      901 catcgcgctg ctccgtcacc ggcggcggac cgggatcgac gatgacggtg acccgcgtct
      961 cctggacctt tcccgcgaag ctgtagagcg cgatcttcgt cgcggcgaac gcctcgccgc
     1021 cgacggtggt cagcttcagg acatccgcgt cgaggtagcg gatatcgatg ccggagccgg
     1081 tcaccatccg gatctggttc gaggcgccaa ctggcgagcc ggcgtcggcg cgggaataga
     1141 tcgcgaagcc gccatacccg agaacgtgct gtgcctgttc gccggcgagc agggtcgaac
     1201 tgaccccgcc accggggtag ggcagcacct ggatgtcctc gcttgtgccg agcgcgatca
     1261 ggatatcgcc gttgtagctt gcgacatcgg tgatcgcctg gccggcgccg gcgtcccaga
     1321 cctgggtttc gggtgcccag gtcgtcgcat cgagggcgac cgttcgatag aggtatcgtc
     1381 cgcgagcgaa gaagacgtga ttgccgatcg tcgcggccgg aaatggcgtc gcggtcgtcg
     1441 ggagatcgac gccggaggcg atcgtggcgg ttgcggtaag gggccatggg acgacgccct
     1501 gcccaccgag cgcgggaccg atcgccagcg agtcgccgcc gcggtcccgt tcgagctgga
     1561 aggcgcggtg ctggccgccg aagaagtccc ggatctggat ccgggcagtc cggccttccg
     1621 ccatcccgtc ctgcgcccgg gtataggtcg cgttcgcacc cgttgccggc acgatcatgt
     1681 acccggcgcc gtcgagcacg atatccgttg cctggggcat cgtcgcacct ccttctggat
     1741 gattggctgt cgtcccggcg accacgtgcc tgcccgtccc ggcgacccac gtggtcgcct
     1801 atcaaacgcc gcacgcaggc tcaggcccag ccggtgattc gcgtctccca ttccgtccac
     1861 gtcgtccagc gaagtcgcca gtcctcgacg tagcgcgtgc gccattccgt ccaggccagg
     1921 actcgccggc accggcagtt cacgtgaacg gggggatgtg gtcccgatcc ttcttcccag
     1981 atcgccccgt agatcgggcc gcactccggg cagacgcgct cgtccgcggc ggtctcccag
     2041 cgttcgaaga tgaccggata gacgatggcg accttctcga ggacccagag ccaggcaccg
     2101 acgacctcct cgacgaactc gatgaccggt tcgaggagat cggggatgcg atcgcgcacg
     2161 tagtcgacgg tgtcattcca ggcatcccgc acgg
//
LOCUS       DASTMD010000621         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_682538, whole genome shotgun sequence.
ACCESSION   DASTMD010000621 DASTMD010000000
VERSION     DASTMD010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682538"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFQ54_09175"
     CDS             <1..685
                     /locus_tag="VFQ54_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0165206.1"
                     /translation="DLLILCNPHNPIGRVLTKDEMVKISEIVDRHNGRVFSDEIHAPL
                     RYEGQTHVPYASVNGVAAGHTITAIAASKAWNLAGLKCAQLILSNEADAARWKEIGMF
                     AGHGASTVGVIASIAAYNDGREWLAEITEYLDGNRRYLSDLVAQHLPGVVYNQPQGTY
                     LAWLDFRNTGLDGNLAEFFREHAKVALTDGGACGEIGQRHIRFNFAMPRPLLETAVTR
                     MGGALASFY"
     gene            719..1525
                     /locus_tag="VFQ54_09180"
     CDS             719..1525
                     /locus_tag="VFQ54_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0165207.1"
                     /translation="MAIVQQWFDVRELEDGVFLIVEPFHDEQVKSHLIVGADRAILLD
                     TGMGVGNIREVAEGLTVKPISVVNSHAHWDHIGGNHLFEEIAIHPAEADELLAGAPNA
                     TLRPWFQADSLSGPLPDGVTAETISIPPSRATSLLNYSDVIDLGGVRLQVMHCPGHSA
                     GGIVLFDRVRGILFSTDVAYAGALYVYSVPDLAIYQHSLARLAAIAPGLRVCYPCHNA
                     TPMSPEILPKMADGLEAIVDGKAPDLVRGQVSAWRFDGFSVELKLGRDEE"
BASE COUNT          297 a          508 c          529 g          295 t
ORIGIN      
        1 tgacctgctg atcctctgca atccccacaa cccgatcggt cgcgtcctga cgaaggacga
       61 gatggtgaag atcagcgaga tcgtcgatcg gcacaacgga cgcgtcttct cggacgagat
      121 ccacgcgcca ttgcgatacg aggggcaaac tcacgttcct tatgcctcgg tcaacggtgt
      181 cgccgccggt cacacgatca cggcgatcgc ggcctcgaag gcgtggaacc tggccggtct
      241 caagtgcgcg cagctcatcc tcagcaacga ggcggacgcc gcgaggtgga aagagatcgg
      301 gatgttcgcc ggtcacggcg cgtcgacggt tggggtgatc gcctcgatcg ccgcatacaa
      361 cgatgggcgc gagtggctcg ccgagatcac cgagtatctc gacggaaacc gtcgctatct
      421 ctccgatctg gtcgcgcagc atctgccggg tgtcgtctac aaccagccgc agggaaccta
      481 tcttgcctgg ctcgacttcc ggaacacagg tcttgacggc aatctcgccg aattcttccg
      541 tgagcacgcg aaggtcgcat tgaccgacgg cggcgcttgc ggtgaaatcg gccagcgtca
      601 cattcgcttc aacttcgcga tgccgcggcc actgctggag accgccgtga cgcgcatggg
      661 tggcgcgctg gcgtccttct actagggact tcgatcgcga agagagcagg ggaacaacgt
      721 ggcgatcgtc cagcagtggt tcgatgtgcg cgagttggag gatggcgtct tcctgatcgt
      781 ggagccattc cacgacgagc aggtgaagtc gcatctcatc gtcggtgcgg accgggcgat
      841 cctgctcgac accggaatgg gcgttggcaa catccgcgag gtcgcagaag gcctgacggt
      901 aaagccgatc tcggtcgtca acagtcacgc ccactgggat cacatcggcg gcaaccacct
      961 cttcgaggag atcgcgattc atcccgccga ggcggacgag ctgctcgcgg gagcaccgaa
     1021 cgcgacgctg cgaccgtggt ttcaggcaga ctcgctctcc gggccgctgc cggatggtgt
     1081 gaccgcggag accatctcga ttcctccgag cagggcgaca tcgctgctga attatagcga
     1141 cgtcattgat cttggcggcg tccggctcca ggtgatgcat tgcccgggcc actcggctgg
     1201 cggaatcgtg ctgttcgacc gggtgcgcgg gattctcttc agcacggacg tcgcctacgc
     1261 cggggcgctc tacgtctaca gcgtgccgga cctcgcgatc taccaacatt cgctcgcccg
     1321 gctcgccgca atcgcgcccg ggttgcgcgt ctgctacccg tgccataacg cgacgccgat
     1381 gagtcccgag atcctgccga agatggcgga tggactcgag gcaatcgttg atggaaaggc
     1441 tccggatctg gtccggggac aggtctcggc ctggcgcttc gacggtttct cggtcgagct
     1501 caagctcggc cgtgacgagg agtagttgcc gcgtccgggc gctggaaacg caccgacaag
     1561 gcatcgtccg agggtgggag ggacgtgaat cccctgcgcg gcggggtata aaaccccgcc
     1621 ctaccaatc
//
LOCUS       DASTMD010000622         3968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1397929, whole genome shotgun sequence.
ACCESSION   DASTMD010000622 DASTMD010000000
VERSION     DASTMD010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397929"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VFQ54_09185"
     CDS             complement(<1..861)
                     /locus_tag="VFQ54_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0165208.1"
                     /translation="MAAIRDNTAPMTSGAFENTVPRPRFSWYRLLEGATPYLLIAPLL
                     ILIAVFIYWPLIYSAYLSFFDWNFVRPNKEFVGFDNYTRLAHDPRFARSLHGTWIYIV
                     TLVPIQVFLPLGLALLLWPIRKAKAQNAYRIMLFSPTVIAYSVGAVMWLWIFNPLQGL
                     LNKLLIDFGFARVNWLSDRDTAIWAVIIVATWKTLGFHLLLYMAALEAVPNDYVEAAS
                     LDGASSWQIMRYIRFPLITPTFFFILVTTVISVNDDVFGAINVLTDGGPFESTTNIIY
                     YLYQQGFKYFQ"
     gene            complement(1016..2305)
                     /locus_tag="VFQ54_09190"
     CDS             complement(1016..2305)
                     /locus_tag="VFQ54_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0165209.1"
                     /translation="MKALSRRSFVAATAAVTATGAASTRFAMPAVIGAQAKPIEITFY
                     HIWGTPPGAAAKSTKHPADQVIDAFNASQTAIKVNAQTPATDYATVEQKLQADLAAGN
                     PPALCTIPWANINYANEGLGIVPLDTIAGSDLKSVTGNILPDVLPLAQIGTQTMGLPY
                     ALSTPIAFYNADILKQAGVDPAVLFKDWESFAQEGPKVQSALGGGNPVLAISYNKDWP
                     SQGIIQSNGGRVLDDSGQPAMNSAEAEAAMQAIADLDKAGLYDRGTSQELRPNWIGGS
                     TAVMISSSAGVGSVKTDATFTWSTAPTPAFAGKKRQMSTGGSFIGCFAKDDDQRAAAW
                     TFLKYANSESATKLWAQTGYLNSTTWDLPVLPGQESGYAQLKEGLTRETPWPGAHGAE
                     MQATWGGYVERIWAEDISVKDGCEEAVSDLKSILAQS"
     gene            complement(2394..3380)
                     /locus_tag="VFQ54_09195"
     CDS             complement(2394..3380)
                     /locus_tag="VFQ54_09195"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU0165210.1"
                     /translation="MAEPHGALLISVARMYYLDGMGQSEIADIFGVSRSTVSRLLTTA
                     RERGIVRISVDEYDPRDHELEQRLTGRFGLRDAIVVRSIEGQAPHVRRAVGYFAAPIV
                     AQRIAAAGSVGVAGGRTLGQLIHFIEPQSHQDHLNVVQLMGTVGSAPSSVDASELSRT
                     LARRFQGSFHTINAPAFMDDPRSRDLLLSHGQMRSVWGMFPTLDLALIGIGSLEDSVF
                     AERNVLAANDLEILRAQGAVGEICGRFFDAAGQECHSQLRERVIGVELETLRSGKEVV
                     AITAGSSRRVAIHAALAGGLVHSLVIDDRGAEAVLAMAGAEVRRHVTEERAS"
     gene            complement(3722..>3968)
                     /locus_tag="VFQ54_09200"
     CDS             complement(3722..>3968)
                     /locus_tag="VFQ54_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165211.1"
                     /translation="GGGPLDTDPKYPDQGSALSALKNSTSWTVQLGWPGPTNSLVAAA
                     VDQSILPTMLARAAKGELAPKDAVAEAEARLKQIAAQ"
BASE COUNT          701 a         1186 c         1363 g          718 t
ORIGIN      
        1 ctggaagtat ttgaatcctt gctggtagag gtaatagatg atgttggtcg tcgattcgaa
       61 ggggccgcca tcggtgagga cgttgatcgc gccgaagaca tcgtcgttca cggagatcac
      121 ggtggtgacg aggatgaaga agaaggtcgg cgtgatcagc gggaagcgga tgtagcgcat
      181 gatctgccag ctggaggcgc cgtccaggga tgccgcctcg acataatcgt tcggtacggc
      241 ttccaacgcc gccatataga gcagcaggtg gaagccgagc gtcttccagg tcgcgacgat
      301 gatcacggcc cagatcgcgg tgtcgcgatc ggagagccag ttgacgcggg cgaagccgaa
      361 atcgatcagc agcttgttca gcagtccctg caggggattg aagatccaga gccacatcac
      421 cgcgcccacc gagtaggcga tgacggtggg agagaagagc atgatgcgat aggcgttctg
      481 cgccttcgcc ttgcggatcg gccagagcag gagcgccagg ccaagcggca ggaagacctg
      541 gatcggtacc agcgtgacga tgtagatcca ggtgccatgg agggagcgcg cgaagcgggg
      601 atcgtgggca agccgcgtgt agttgtcgaa tccgacgaac tccttgttcg gccgcacgaa
      661 gttccagtcg aagaaggaga ggtaggcgga atagatgagc ggccagtaga tgaagacggc
      721 gatcaggatc agcagggggg cgatcaggag gtacggagtg gcgccctcga gcaggcgata
      781 ccaggaaaat cgcggtcgag gaaccgtgtt ttcgaatgcg cctgaggtca tcggcgcggt
      841 attgtcgcgg atggccgcca tgaaatctcc ttgcgtgatg cggtctcgtc ggacggaggt
      901 tcgctcgttc ccctccggat cgcgtggccg gaccagacaa ccgtatctgg tccggatgcg
      961 cggcgtatta tcggaacgta aggatgtacg cccgtaacgt aggcattacg aagggtcagg
     1021 actgggcaag gatgctcttc aggtcgctga cggcttcttc gcagccatcc ttgacgctga
     1081 tgtcctcggc ccagatccgc tcgacgtaac caccccaagt ggcctgcatc tcggcgccgt
     1141 gcgcgcccgg ccacggggtc tcgcgggtga gtccttcctt gagctgggcg tagccggatt
     1201 cctggcccgg aaggaccggg agatcccacg tcgtggagtt gagatagccg gtctgcgccc
     1261 agagcttggt cgcggattcg gagttggcgt acttcaggaa tgtccaggcg gcggcgcgct
     1321 ggtcatcgtc cttggcaaag cagccgatga aggagccgcc ggtggacatc tggcgcttct
     1381 tgccggcgaa ggccggggtc ggcgcggtgc tccaggtgaa ggtggcgtcc gtcttcacgg
     1441 agccgacgcc ggcgctggac gagatcatga cggcggtcga gccaccgatc cagttcgggc
     1501 ggagctcctg gctcgtgccg cgatcgtaga gaccggcctt gtcgagatcg gcgatcgcct
     1561 gcatcgcggc ctccgcctcg gcggagttca tcgccggctg accgctgtcg tcgaggacgc
     1621 ggccaccgtt cgactggatg atgccctggg acggccagtc cttgttgtag ctgatggcga
     1681 gcaccggatt accaccgccg agcgcggact ggaccttcgg tccctcctgc gcgaagctct
     1741 cccaatcctt gaacagcacc gccggatcga cgccagcctg cttgaggata tcggcgttgt
     1801 agaaggcgat cggcgtcgag agcgcgtagg gcagacccat cgtctgggtc ccgatctggg
     1861 cgagcggaag gacatccggc aggatgttgc cggtcaccga cttgaggtcg ctgccggcga
     1921 tggtgtccag cggcacgatg ccgagaccct cgttggcata gttgatgttc gcccacggga
     1981 tcgtgcagag cgccggcggg ttgccggccg cgaggtcggc ctgcagcttc tgctcgaccg
     2041 tcgcgtagtc ggtcgccggc gtctgggcgt tgaccttgat cgcggtctgg ctggcattga
     2101 aggcgtcgat cacctggtcg gccgggtgct tggtcgactt cgcggcggcg cccggcggcg
     2161 taccccagat gtgatagaag gtgatctcga tcggcttggc ctgggcgccg atcacggccg
     2221 gcatcgcgaa gcgcgtggag gcggcaccgg tggccgtgac ggcggcggtg gcggcaacga
     2281 atgaacgtcg ggaaagtgct ttcatggttc tggttcctct gatctgaatg aacgtctgat
     2341 tgttcgcacc tgagctgttg ctgacgatcg atcgcatgtg ggcgggagat cggctacgac
     2401 gcccgctcct cggtgacgtg ccggcgaacc tccgcgcccg ccatcgcgag gacggcttcc
     2461 gcgccccggt cgtcgatcac cagggagtgc acgagaccac ccgcgagcgc cgcgtggatc
     2521 gcgacccggc gggacgatcc cgcggtaatc gcgacgactt ccttgccgga gcgaagcgtt
     2581 tccagctcca ccccgatcac ccgctcccgc agttgggagt gacattcctg tcccgccgcg
     2641 tcgaagaagc gcccgcagat ttccccaacg gcgccctgcg cccgcaggat ctcgaggtcg
     2701 ttcgcggcga ggacatttcg ttccgcgaag acggaatctt cgagggaacc gatcccgatc
     2761 aaggcaagat cgagcgttgg gaacatgccc cacaccgagc gcatctgacc gtgggagagg
     2821 aggagatcgc ggctccgggg atcgtccatg aaggccggcg cgttgatggt gtggaacgac
     2881 ccctggaacc ggcgcgcaag cgtgcggctg agttccgagg cgtcgacact gctcggcgca
     2941 gagccgacgg ttcccatcag ctggacgaca ttcaggtgat cctggtggga ttgcggctcg
     3001 atgaagtgga tgagttgacc gagggtgcgg ccgccggcga cgccgaccga cccggcagcg
     3061 gcgatccgct gcgccacgat cggggcggca aagtacccca cggcccgccg tacgtgcggc
     3121 gcctgaccct cgatgctgcg caccacgatt gcgtctcgca gtccgaagcg tccggtcagg
     3181 cgttgttcca gctcgtgatc gcgcgggtcg tactcatcga ccgagatccg gacgattccc
     3241 cgttcgcgtg ccgttgtcag caggcgggag accgtcgagc gggagacacc gaagatatcg
     3301 gcgatctcgc tctgtcccat accgtccagg taatacatgc gtgccacgga aatcagcagg
     3361 gcaccatgtg gttccgccat cgtcctcccc aatccacctg cacatatgtg cgcgcacaga
     3421 atttcgtcga tgggaacacg caattcacaa cgcccgatct ccggcgatcg gcgagagctc
     3481 ccgtgcacat ccgtgcgaca acaactgtgc agttcgggat aaggggaatt gctaaatcgg
     3541 tattgccatc gactgcacgt tcgtgcaggt ttgtgtatcg gtaagcgatg cgtggatatc
     3601 gtttgtgacg cgtgcgccat gaggctgggc caacccgtat ggtcgcccgt tctggaggct
     3661 catctcgcga cgtcaagagc gcacacaccg tggcctggtg cggctggccg gatagagcgg
     3721 cctattgcgc ggcgatctgt ttcagacgcg cttccgcttc cgccacggcg tccttcgggg
     3781 cgagctcacc cttcgcggcg cgcgccagca tcgtcggcag gatcgactgg tcgaccgccg
     3841 ccgcaacgag cgaattcgtc gggcctggcc agccgagctg cacggtccag gaggtcgagt
     3901 tcttcagcgc actcaacgcg cttccctggt cgggatattt cgggtcggtg tcgagcggac
     3961 caccgccg
//
LOCUS       DASTMD010000623         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2380139, whole genome shotgun sequence.
ACCESSION   DASTMD010000623 DASTMD010000000
VERSION     DASTMD010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2380139"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="VFQ54_09205"
     CDS             complement(<1..1563)
                     /locus_tag="VFQ54_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0165212.1"
                     /translation="MPAPVEPMNISRYFIDRPIFAAVLSFAIFISGLIAILQLPISEY
                     PEAVPPQVVVTAQFPGANPKIIAETVATPLEEQMNGIDNLLYTDSQATSDGSMKLTAT
                     FKIGTRPEEAETAVQNRINRALPRLPEVTRQIGVTTERSSPNLAMVVHLVSPDGRYDA
                     LYLRNYAVLHVRDQLLRLRGVGQTWLAGSGDYAMRIWLNPAQLSARGLSTSDVVDAIR
                     EQNLQVAAGVIGAPPGPDAPDFQLAVSAQGRLTTEDEFSEITVRSAPDGSTIRVRDVG
                     RVELGQSTYAVRSLLHNEDAFGVAIFQAPDSNALQMSKDVRAEMEVLAKDFPPGLEYR
                     IIYDTTAFVSASIESVIHTLFEAVALVVLVVILFLQSWRAAIIPLLTVPVAAVGTFGV
                     LLLLGYSINTLTLFALVLSIGIVVDDAIVVVENVERHMELGKSAHDAAVLAMQEVSGP
                     IIAIALVLCAVFVPLAFVSGLSGQFFRQFAVTIAISTVISAFNSLTLSPTLAARLLVD
                     HAAPQDRLQRFID"
     gene            complement(1547..>2226)
                     /locus_tag="VFQ54_09210"
     CDS             complement(1547..>2226)
                     /locus_tag="VFQ54_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0165213.1"
                     /translation="LNLGYTEIRAPIAGRVSRALITEGNLVAAGTQVLATIESLDTVY
                     AYFDASEAVFLRFTKQGRGPLKSAAEVIPIQLGLLDEPGYPHAGRIESFDNRINVDTG
                     AIRTRAVFDNRKREFTPGLYARLRLVSGPAVPATLIPDRAIETDQSKKFVMVVGSDDV
                     AAYRDVQPGALQDGLRVIVAGLKPGERVVVDGLQRVRPGAKVAAESVKIDDREEGNPT
                     IAGAGAR"
BASE COUNT          409 a          694 c          748 g          375 t
ORIGIN      
        1 atcgatgaag cgctggagtc ggtcctgcgg cgccgcatga tcgacgagga gccgcgctgc
       61 gagtgtcggc gacagcgtca gcgagttgaa cgccgagatc accgtcgaga tggcgatcgt
      121 gacggcgaac tggcggaaga attgtcccga aagacccgac acgaacgcga gcgggacgaa
      181 cacggcgcac agcacgagcg cgatggcgat gatcggcccc gacacttcct gcatcgccag
      241 gaccgccgca tcgtgagccg acttgccgag ctccatgtgt cgctcgacgt tctcgacgac
      301 gacgatcgcg tcgtcgacga cgatgccgat cgacagcacc agcgcgaaga gcgtcagcgt
      361 gttgatcgaa tacccgagca gaagcaggac gccgaacgtg cccacggccg ccaccgggac
      421 cgtcaacagc gggatgatgg ccgcacgcca ggactggagg aaaaggatga cgacgagcac
      481 gaccagcgcc accgcctcga agagcgtgtg aatcaccgat tcgatcgaag cgctcacgaa
      541 ggccgtcgtg tcgtagatga ttcgatactc gagccctggg ggaaagtcct tggcgagaac
      601 ttccatctcc gcgcggacgt ccttggacat ctgcaaggca ttcgagtccg gcgcctggaa
      661 gatcgcgacc ccgaaggcgt cctcgttgtg aagcagcgaa cggaccgcgt acgtgctttg
      721 cccgagctcg acccgaccga cgtcgcggac gcggatcgtc gagccgtcgg gagcgctccg
      781 aacggtgatc tcgctgaact cgtcctcggt cgtcaggcgt ccttgtgcgc tgaccgcgag
      841 ctggaaatcg ggggcgtcgg gacccggtgg cgcaccgatc acaccggccg cgacctgcag
      901 gttctgctcg cgaatcgcgt cgaccacgtc gctggtggac aagccgcgag ccgatagctg
      961 cgccgggttg agccagatcc gcatggcgta gtcgccggac ccggccaacc acgtctgccc
     1021 gacgccgcgg agccgaagga gctggtcccg gacgtggagc accgcgtagt tgcgaaggta
     1081 gagagcgtcg tagcggccgt cgggcgagac gagatgaacg accatcgcga ggttcggcga
     1141 actgcgctcg gtcgtcacgc cgatctgtcg cgtgacttcc ggaagccgcg gcagcgcccg
     1201 attgatccgg ttctgaacgg cggtctccgc ctcctcggga cgtgttccga tcttgaaggt
     1261 ggccgtcagc ttcatcgatc cgtcgctggt cgcctggctg tcggtgtaga gcagattgtc
     1321 gatcccgttc atctgctcct cgagcggcgt cgcgaccgtc tcggcgatga tcttggggtt
     1381 cgcaccgggg aactgggcgg tgacgacgac ctgcggaggg acagcttccg gatattccga
     1441 gatcggcagc tgcaggatgg cgatcaagcc actgatgaag atggcgaacg aaagaactgc
     1501 cgcgaagatc ggccggtcga tgaagtagcg cgagatgttc atcggctcaa cgggcgccgg
     1561 caccggcgat ggtcgggttg ccttcttcgc gatcgtcgat cttcacggac tccgcggcga
     1621 ccttggcgcc gggtcgcacc cgttgcaggc catcgaccac cacacgctcg ccgggcttca
     1681 aacctgcgac gatcacgcgt agtccgtcct ggagcgcccc tggctgcacg tcgcggtacg
     1741 ccgcgacgtc atccgatccg acgaccatga cgaacttctt gctctggtcc gtttcgatgg
     1801 cccggtcggg aatcagcgtg gcaggtacag ccggtccgga gaccagccgc aatcgggcat
     1861 agagccccgg ggtgaactcg cgcttccggt tgtcgaaaac cgccctcgtg cgaatcgcac
     1921 cggtatcgac gttgatgcga ttgtcgaagg actcgatgcg tccggcatgc ggatagcccg
     1981 gctcgtcgag caagcccagt tggatcggga tgacctccgc cgcagacttg agtggtcccc
     2041 ttccctgctt cgtgaatcga aggaagacgg cttcgctggc atcgaagtag gcgtagacgg
     2101 tgtcgagcga ctcgatggtc gccagcacct gtgtgccggc cgcgacgaga ttgccctccg
     2161 tgatcagtgc gcgtgagact cgtccggcga tgggcgcgcg gatttccgtg tagccgagat
     2221 tgaggt
//
LOCUS       DASTMD010000624         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2826482, whole genome shotgun sequence.
ACCESSION   DASTMD010000624 DASTMD010000000
VERSION     DASTMD010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2826482"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1472)
                     /gene="lpdA"
                     /locus_tag="VFQ54_09215"
     CDS             complement(48..1472)
                     /gene="lpdA"
                     /locus_tag="VFQ54_09215"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU0165214.1"
                     /translation="MAESGQNQDNQFDIVLLGGGTGGYVAAIRAKQLGFNVAVVEAVK
                     LGGTCLHRGCIPTKAMLKSASVLDQVKESAEYGVTLTGEIGFEYKTALERSLKVVEGQ
                     YRGLQYLFDKKHKIPVFMGRGKILDASTVEVTPNDGGAVFSLKATDIIINTGSRPRGI
                     KGIEFDGKRVFNSDHSVVREDMPKSVIVRGGGATGVEWADIYHRYGAKVTLVGRIVPQ
                     EDAEVSQHIQRAFQRKKLDVIPDARPAAEDIDVTDSGVTVRITDSKGKQRVLEAESLF
                     VAIGRQGNVENIGLENVGVTVEGDIIPVDSMLRTNVPHIYAIGDVNGQQMLAHTAMHH
                     GIIAVEHIAGKNPFPLDVLNSASCTYCEPEIGSVGLTESQAKELGYEIKIGKFPMKPN
                     AKATIEGHTDGFTKLIVDADTDDILGVHIVGPHATELIAEAGLARFLNASVQELATTV
                     HPHPTVSEVLGEAAHDALGHAIHI"
     gene            complement(1611..>2017)
                     /locus_tag="VFQ54_09220"
     CDS             complement(1611..>2017)
                     /locus_tag="VFQ54_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165215.1"
                     /translation="SPITPIVGSGKSLFQPIQVDDVAACFVRAVDDPELTTGQTYELG
                     GGRAYTYQAMVGEIGRAIGKSPPRVHIPTPLMRAVVGATNPLPRALRPPVTTEQLKML
                     SLDNSTPNSATEALIGRKPIALEDGIGYLRKS"
BASE COUNT          326 a          621 c          660 g          410 t
ORIGIN      
        1 tcacccgttt ctgattgcgc gtccgtctgg aaccgatccg cgatcgatca gatgtggatc
       61 gcgtgtccca gcgcatcgtg cgccgcctcg ccgaggacct cggagacggt cggatgcggg
      121 tgaacggtcg tcgccaactc ctggacgctc gcgttgagga agcgggctag gccggcctcg
      181 gcgatcagtt ccgtggcgtg cggccccacg atgtggacgc cgaggatgtc gtccgtatcg
      241 gcgtcgacga tcagcttggt gaagccgtcg gtgtgcccct cgatcgtcgc cttcgcgttc
      301 ggcttcatcg ggaacttgcc gatcttgatc tcgtagccga gttccttcgc ctgcgactcg
      361 gtgagcccga ccgagccgat ctccggttcg cagtaggtgc agctcgccga gttgaggacg
      421 tccagcggga acggattctt gccggcgata tgctcgacgg caatgatgcc gtgatgcatc
      481 gcggtgtggg cgagcatctg ctggccgtta acgtcgccaa tcgcatagat gtgcggcacg
      541 ttggtgcgca gcatcgagtc gaccgggatg atgtcgcctt cgacggtgac acccacgttc
      601 tccagcccga tgttctcgac attgccctgg cgtccaatcg ccacgaagag cgactccgct
      661 tcgagcacgc gctgcttgcc cttggaatcg gtgatgcgga cggtgacgcc actatcggtg
      721 acgtcgatgt cttccgccgc cggccgggcg tccggaatca catcgagctt cttgcgctgg
      781 aaggcgcgct ggatgtgctg ggaaacctcg gcgtcctcct gcgggacgat gcgtccgacg
      841 agggtgacct tggcgccgta gcggtggtag atgtccgccc actcgacgcc cgtcgcgccg
      901 ccgccacgga cgatgacgct cttcggcatg tcctcacgta cgacggagtg gtcgctgttg
      961 aagacgcgct tgccgtcgaa ctcgatgccc ttgatccccc gaggacggga gccggtgttg
     1021 atgatgatgt cggtcgcctt caggctgaag acggcgccgc catcattcgg cgtgacttcg
     1081 accgtggacg cgtcgagaat cttgccgcgt cccatgaaga ccgggatctt gtgcttcttg
     1141 tcgaagaggt attggagacc acggtactgg ccctcgacga ccttgagcga gcgctcgagt
     1201 gccgtcttgt actcgaaacc gatctcgccg gtgagggtga cgccgtactc ggcggactcc
     1261 ttcacctggt cgagcacgct ggcggatttc aacattgcct tggtggggat gcatccgcgg
     1321 tggaggcagg ttccacccaa cttgaccgct tcgacgaccg cgacgttgaa tccgagctgc
     1381 ttcgcccgga tggcggcgac gtagccgcca gtgcctcctc cgagcaggac gatatcaaac
     1441 tgattgtcct gattctgtcc tgattccgcc atgaaagagt tctctccagt cgcttggacg
     1501 gttatcggcc gggccctccg ttggacccga ttgccgttct tttggatatt atccaataat
     1561 cgcgcaagga cacagagaag aatgggggag cgagccggcc cggcgtatgg tcaggacttc
     1621 cggagatagc cgatcccgtc ctcaagtgcg atcggcttcc ggccaatcag cgcttcggtc
     1681 gccgagttcg gggtgctgtt gtcgagggag agcatcttca gctgctccgt cgtcaccggc
     1741 ggccgaagcg cgcgcggtag cggattcgtg gcgccgacga cggcacgcat gagcggcgtc
     1801 gggatgtgaa cccggggggg agacttgccg atcgcgcgac cgatctcccc gaccatcgcc
     1861 tgataggtgt aggcgcgacc gccgccgagt tcataggtct ggcctgtggt gagctccggg
     1921 tcatcgacag ccctgacgaa gcacgctgcc acgtcatcga cctggatcgg ctggaacagc
     1981 gacttccccg atccgacgat cggtgtgatc ggggacc
//
LOCUS       DASTMD010000625         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2201361, whole genome shotgun sequence.
ACCESSION   DASTMD010000625 DASTMD010000000
VERSION     DASTMD010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2201361"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1161)
                     /locus_tag="VFQ54_09225"
     CDS             complement(103..1161)
                     /locus_tag="VFQ54_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU0165216.1"
                     /translation="MNEHQKDFLRKLITTPSPSGFEERASEVWRAEASTFADSVEWDV
                     LGNSYARLANESGPKVVIEGHIDEIGFIVTYIDDEGFIWFDKIGGWDDQVIVGQRITI
                     AGPGGDVKGVIGKKASHLLTPADREKVTKSSDLWIDIGAATRAEAETRVSVGDAAVVD
                     VLPIELTDDLWASRSMDNRVGAFVALEALRLLADERPIADVYAVSGTQEEIGLVGATH
                     LSFRLSPAVGIAIDVTHSTDYPGASPKADNKVRLGGGPVLTRGAVINPKVYEGLREAA
                     KRLDIPYAVQAAARVSATDADAFTRSGAGVAAGLVSIPNRYMHSPNEVISLSDLESAS
                     RVIAEFVRTITPESDFRR"
     misc_binding    1343..1610
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1676..>3049
                     /locus_tag="VFQ54_09230"
     CDS             1676..>3049
                     /locus_tag="VFQ54_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEU0165217.1"
                     /translation="MVAEVSSERSQGTAVDIQPSLAQVRTIAETVAPDIRTVPIYREI
                     LADLETPVSVYLKLSDGARLPGFLLESVEGGIRIARYSFIGAGDYTSLTMVDGTLTIS
                     GRDGETTAPYADPLTALTGILEGYKAARDSDLPRFTGGVVGYLGYESVRRFEPRVGRA
                     KGPGLGLPEAKYHLADSLVVFDHLQRSMKVVAHVRLDTGDLEASYQKAVADIDALTAK
                     LHAATPEYDLTPSSQTAAVVDRFRPNTSPERYREMVQTVKQYIYDGDIIQCVPSQRID
                     IDTEAHPFTIYRALRVVNPSPYMFFLDFGDFHVVGASPELLVRSEDGIITNHPIAGTR
                     PRGQTPTEDVALAEELIADEKERAEHIMLVDLGRNDVGRVSKPGTVRVPRLMEIERFS
                     HVMHIVSSVEGQLRDDLTSLDALRACFPAGTVSGAPKIRAMEIIASLETDLRGVYSGA
                     VGYVDFAG"
BASE COUNT          529 a          951 c          991 g          578 t
ORIGIN      
        1 agttgctgta tcccagaccc ggcgtcggaa tcgatgccgg gtctggcgat gtcgcaccga
       61 ttcgaaggcg tgacgtatag actctatggg ccgcgatacc tcctagcgac ggaagtcgct
      121 ttccggcgtg atcgtgcgca cgaactcggc gatgacgcgc gaggccgatt cgagatcgga
      181 gaggctgatc acctcgttcg ggctgtgcat gtagcggttc gggatcgaga cgagacccgc
      241 cgcgactccg gctccggagc gtgtgaaggc gtcggcatcg gtggcgctga cgcgcgcggc
      301 cgcctggacg gcgtagggga tgtcgagtcg cttcgcggcc tcacgcagcc cctcgtagac
      361 cttcggattg atcacggcgc cccgggtgag caccggaccg ccgccgagcc tgaccttgtt
      421 atcggccttc gggctggcgc ccggatagtc cgtcgagtgc gtgacatcga tcgcgatgcc
      481 gacggccggg gagagccgga acgagagatg cgtcgcgccg acgaggccga tctcttcctg
      541 ggttccggag acggcataga cgtccgcgat cggtcgctcg tccgcgagca ggcgcagcgc
      601 ctccagcgcg acgaaggcgc cgacgcgatt gtccatgctt cgtgaggccc agagatcgtc
      661 cgtcaattcg atggggagga cgtcgacgac ggccgcgtca ccgacgctga ccctggtctc
      721 tgcctcggct cgcgtcgcgg ccccgatgtc gatccagaga tcgctggatt tcgtcacctt
      781 ctcgcggtcg gccggcgtga gcaggtggga ggctttcttg ccgatcacgc ccttcacgtc
      841 accaccggga cctgcaatcg tgatgcgctg cccgacgatc acctgatcgt cccacccgcc
      901 gatcttgtcg aaccagatga agccctcgtc atcgatgtag gtaacgatga agccgatctc
      961 gtcgatgtgg ccctcgatga cgaccttcgg gccggactcg ttcgcgagac gtgcgtagga
     1021 gttgccgagg acatcccact cgacgctgtc ggcgaaggtg gatgcttcgg cgcgccagac
     1081 ctcggatgcg cgttcctcga agccggacgg gctcggcgtc gtgatcagtt tccgcagaaa
     1141 gtccttctgg tgctcgttca ttggggattc cctttctgat gtcgccagtc ctgactggct
     1201 cggtcaatct gcgcagacta tcacggggcg ggcagaagcc gatatgttgg cgcatgcacg
     1261 attgcacgag gcgagcggcc gaagtttgtg cagacttgac atcgatcagg cggtgccgta
     1321 ctgtgcgcac aacaactgaa tgcgaacagc gatgatcgga acgagtagac gtcgtcacat
     1381 cgatcgaagc gagccgggga gggtgaaagc ccggcgtgag atgggcgtcg aatggatccg
     1441 ggagctgtgc cccaaaacgc cttcgggcgg agtagacggc aacggtgtct cccccgttat
     1501 caggagcctc gtatcggttc attcgggaac ccgtacggtt tgagtgggtg gtgtatcggc
     1561 atcgccaatg agggtggcac cgcggaaggc tcacagcctc cgtccctttt gtgggacggg
     1621 ggttttttgc gtccgtccgt tggatgaatg tcatccggga aggttgatcg aatcgatggt
     1681 tgcagaggtt tcatcggagc gttcgcaggg gacggcggtc gacatccagc cttcccttgc
     1741 ccaggtcagg acgatcgcag agacggtggc gccggacatc cggacagtgc cgatctatcg
     1801 tgagatcctt gccgatctcg aaacacccgt ttcggtctat ctcaagctca gcgatggcgc
     1861 ccggttgccc ggattccttc tcgaatcggt ggagggagga atccggatcg cgcggtattc
     1921 cttcatcggc gccggcgatt acacgtcgtt gacgatggtc gacggaacac tgacgatctc
     1981 cggtcgggac ggcgagacga ctgctcccta cgccgatccc ctgacggccc tcaccggaat
     2041 tctcgaggga tacaaggcgg cgcgggacag cgatctgccg cggttcaccg gtggcgtcgt
     2101 cggctacctc ggctatgagt cggtgcgccg gttcgaaccc cgggtcggac gcgcgaaggg
     2161 accgggactc ggccttcccg aagcgaagta ccatctcgcc gattccctgg tcgtcttcga
     2221 ccatctccag cgttcgatga aggtcgtcgc ccacgtccgt ctcgacaccg gcgatctgga
     2281 ggcttcgtac cagaaggcgg ttgcggacat cgacgcactc accgcgaaac tgcacgccgc
     2341 gacaccggaa tacgacctca caccgtccag ccagaccgcc gcggttgtcg atcgcttccg
     2401 gccgaatacc tctccggagc ggtaccgcga gatggtgcag acggtgaagc agtacatcta
     2461 cgacggcgac atcatccagt gcgttccgtc ccagcgcatc gacatcgata ccgaggccca
     2521 cccgttcacg atctatcgcg cattgcgggt cgtgaacccg tcgccgtaca tgttcttcct
     2581 cgacttcggc gacttccacg tcgtcggtgc ttcgccagag ctgctcgtcc gctccgagga
     2641 cgggatcatc accaaccacc cgatcgcggg aacgcgaccg cgtgggcaga cacccaccga
     2701 ggatgtcgca ctcgcggagg aactgatcgc ggacgagaag gagcgcgccg agcacatcat
     2761 gctcgtcgat ctcggacgca acgacgtcgg gcgtgtctcg aagccgggga ccgttcgcgt
     2821 gccgcgcctg atggagatcg agcggttctc ccatgtgatg cacatcgtca gtagcgtcga
     2881 aggccagctt cgtgatgacc tgacgagcct cgacgccctg cgcgcctgct tcccagccgg
     2941 tacggtatcg ggtgctccca agatccgggc gatggagatc atcgcgtcgc tggagaccga
     3001 tcttcgcggt gtctattccg gcgctgtcgg ctacgtcgac ttcgccggc
//
LOCUS       DASTMD010000626         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_237298, whole genome shotgun sequence.
ACCESSION   DASTMD010000626 DASTMD010000000
VERSION     DASTMD010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237298"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1585
                     /gene="trpB"
                     /locus_tag="VFQ54_09235"
     CDS             329..1585
                     /gene="trpB"
                     /locus_tag="VFQ54_09235"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU0165218.1"
                     /translation="MAQIPNVVPPVTQAAFSTTTVLPDAHGRFGRFGGTFAPETLMPA
                     ITELIAGYEEAKADPAFQAEIDRLATYYVGRPTPLYEATRFAEEVGGSDGPRIFLKRE
                     DLAHTGAHKINNALGQALLAKRMGKPRIIAETGAGQHGVATATACALLGLKCVVYMGE
                     VDVARQSLNVFRMKLLGAEVISVTSGSKTLKDAINEAIRDWVTNVDDTFYIFGTVAGM
                     HPYPMIVRDFQSVIGREARAQIQEQTGQLPDAIVACVGGGSNAMGLFYPFRNDLSVEI
                     VGVEAGGYGLESGKHAATLVKGEEGVLHGSWSYVLQDDDGQIIETHSISAGLDYPGVG
                     PELSHMKYTGRARFEAITDDEALEGFKLLCRTEGIIPALESSHAIAYAAKFAKELGAG
                     KTMIVNLSGRGDKDMHTVADALGVTL"
     gene            1585..2406
                     /gene="trpA"
                     /locus_tag="VFQ54_09240"
     CDS             1585..2406
                     /gene="trpA"
                     /locus_tag="VFQ54_09240"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU0165219.1"
                     /translation="MATSTNVIPSRISGTFARLAAEGKTALMPYATAGYPDLQTSEDV
                     ISALVQGGADLVEIGVPFSDPLADGAAVQRTSQIALENGTRVRDCIALVKRLRDRGVT
                     VPLLLMGYFNPLLHYGIDRYVADCAEAGVDGFIVPDLPAEESEALQAACVANGRDLIF
                     MIAPTSTDDRIIEIARRGSGFIYCVSVTGVTGARDKMSDTLGEYLNRIRSHTDLPLAV
                     GFGISKPDHVRQVGEHAQGAIVGAALIDYINQVPDAEKPATAARFVKYLRGEGEI"
     gene            2631..>3392
                     /locus_tag="VFQ54_09245"
     CDS             2631..>3392
                     /locus_tag="VFQ54_09245"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0165220.1"
                     /translation="MNRASSGSVVRSFNRRRAIQFGAGGALGSGILSSRIAATTAQSA
                     TPSPVAAEIPISGVANASFATFDRILTDAMVTWSIPGGQLAIGREGRLAYSRGYGAAS
                     LEDREDVQLSSRFRIASTSKPFTGVAILMLVDAGKLTLDTPVFPLLGLTPANNSPKDS
                     RLDTITVEHLLVHAGGWDSAGSGMDPQYLPWTLLASNTLSAENPAEAATIIRFMQSEP
                     LDFDPGTVSSYSNFGFNVLGRVIEHISGMDYDEFVQ"
BASE COUNT          600 a         1165 c         1040 g          587 t
ORIGIN      
        1 acgttctcgc cacaaaccca atattcgggc aaggcgccct ggcgcatgcg ttcgtccacg
       61 aacgggtcgg cggtgcctac ccgaggcgac cacatgggtc gccgggaggg gaatcaccgc
      121 acgttcgcga tttacggctc ccccaaagtg gcgggtttct tgcctcgcct gccggcgcga
      181 aagtcgtgca tgtaccaggg atacgcgcgg gaaggcgtcg cggagtgacg aatccttggt
      241 aaagtaacgg gacgttgcga ccctctccga gagggtgccc ccgccgatgc cgaagggccg
      301 gcgagaatcg ttgatggagc acgtcacctt ggcccagatt cccaatgtcg tccccccggt
      361 tacccaggcc gccttctcca cgaccaccgt ccttcccgat gcccacgggc gcttcggacg
      421 cttcggtggc accttcgcgc ccgagaccct gatgccggcg atcaccgagc tgatcgcggg
      481 ctacgaggag gcgaaggccg atcccgcctt ccaggccgag atcgaccgtc tcgccaccta
      541 ttacgtcggt cgcccgacgc cgctctacga agcgacccgc tttgccgagg aagtcggtgg
      601 cagcgacggt ccgcgcatct tcctcaagcg cgaggatctc gcccacaccg gcgcgcacaa
      661 gatcaacaat gcgctcggcc aggccctgct cgccaagcgg atgggcaagc cccgcatcat
      721 tgccgagacc ggcgccggcc agcacggcgt cgccacggcg accgcctgcg cgctgctcgg
      781 tctcaagtgc gtcgtctaca tgggcgaggt cgacgtcgcg cggcagtcgc tcaacgtctt
      841 ccggatgaag ctgctcggcg ccgaggtcat ctccgtgacg tccggatcga agacgctcaa
      901 ggatgcgatc aacgaggcga tccgcgactg ggtcaccaac gtcgacgaca ccttctacat
      961 cttcggaacg gtcgccggca tgcacccgta tccgatgatc gtccgcgact tccagtccgt
     1021 gatcggtcgc gaagcccgcg cccagatcca ggagcagacc ggtcagctgc cggacgcgat
     1081 cgtcgcctgt gtcggcggcg gctcgaacgc gatgggactc ttctacccct tccgcaacga
     1141 tctctcggtc gagatcgttg gcgtcgaagc gggcggctac gggctcgaat ccggcaagca
     1201 cgccgcgacc ctggtcaagg gtgaggaagg cgtcctccac ggctcctggt cctacgtcct
     1261 ccaggacgac gatggccaga tcatcgagac gcactcgatc tcggccggtc tcgactatcc
     1321 cggcgttggc cccgagctct cgcacatgaa gtacaccggt cgcgcccgct tcgaggcgat
     1381 caccgacgac gaggcgctgg aaggcttcaa gttgctctgc cgcacggagg ggatcatccc
     1441 cgccctcgaa tcgagccatg ccatcgccta cgccgcgaag ttcgcgaaag agctcggcgc
     1501 ggggaagacg atgatcgtca atctctccgg ccgcggggac aaggacatgc ataccgtcgc
     1561 cgacgcgctg ggagtcacgc tctaatggca acatccacga acgtcatccc gagccgcatc
     1621 tccggaacct tcgccagact ggcggcggaa ggcaagaccg cgctgatgcc gtatgcgacg
     1681 gctggttacc ccgacctcca gacgagcgag gatgtgatct ccgccctggt ccagggcggc
     1741 gccgacctcg tcgaaatcgg cgttcccttc tccgacccgc tcgctgatgg cgccgccgtc
     1801 cagcgcacga gccagatcgc cctcgagaac ggcacccgtg ttcgcgactg catcgccctc
     1861 gtcaagcgac tgcgtgatcg cggtgtcacg gttcccctgc tcctgatggg ctacttcaac
     1921 ccgctgctcc actacggcat cgatcgttac gtcgcggatt gcgccgaggc cggtgtcgac
     1981 ggcttcatcg ttcccgatct tccggcggag gaaagcgagg cgctccaggc ggcgtgcgtc
     2041 gccaacgggc gcgacctgat cttcatgatc gccccgacca gtaccgacga ccggatcatc
     2101 gagatcgcgc ggcgtggcag cggcttcatc tattgcgtct ccgttaccgg cgtcaccgga
     2161 gcccgcgaca agatgtcgga tacgttgggc gaatacctca atcgcatccg cagccacacc
     2221 gatctgccac tcgccgtcgg attcggcatc tcgaagccgg atcacgtgcg gcaggtcggc
     2281 gagcacgcgc agggcgcgat cgtcggcgca gcgttgatcg actacatcaa tcaggttccg
     2341 gacgccgaga agcccgcgac tgccgcgcgt ttcgtgaaat accttcgcgg cgagggtgag
     2401 atctagctgc tgacccacga cgttcggtcc gtccctcgcg acggtcgcca ggtgttgtct
     2461 ttcttgggac aaatagacaa caacaccggg acacctgtgt aacgacaatc cccctctgga
     2521 gaactacatt caacaggcgc gttgggttcg tccgatgccg catcatggga aacgccggtc
     2581 gcggcccgca tccgcgactg gtggttcccc tccaggtcag gggagcacgc gtgaatcgag
     2641 cctccagcgg gtccgttgtc cggtcgttca atcgtcgacg cgcgatccag ttcggagccg
     2701 gcggcgcgct cgggagcggt atcttgagtt cccggatcgc cgcgacgact gctcaatccg
     2761 ccaccccttc cccggtagcc gccgagatcc cgatctctgg cgtcgccaac gcgtcgttcg
     2821 cgaccttcga ccggatcctc accgacgcca tggtgacctg gtcgattccc ggtggccaac
     2881 tcgcgatcgg acgagagggg cgtctcgcct atagccgggg ctatggcgcg gcgagcctcg
     2941 aagatcgcga agacgtccag ctttcctccc ggtttcggat cgccagcacg agcaagccgt
     3001 tcaccggcgt cgcgatcctc atgttggtcg atgccgggaa gttgacactg gacactcccg
     3061 tcttcccgtt gctgggactg acgcccgcaa acaacagtcc gaaggattcg cgactcgaca
     3121 cgatcacggt cgagcacctc ctggttcatg ccgggggatg ggacagcgcc gggagcggga
     3181 tggatccgca atacctcccg tggaccctgc tcgcgtccaa tacgctgagc gcggaaaatc
     3241 cggccgaggc cgcgaccatc atccgattca tgcaatccga accactggac ttcgacccgg
     3301 gcaccgtcag ttcctactcg aacttcggct tcaacgttct cgggcgggtg atcgagcata
     3361 tctcgggcat ggactacgac gagttcgtgc ag
//
LOCUS       DASTMD010000627         3059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2649634, whole genome shotgun sequence.
ACCESSION   DASTMD010000627 DASTMD010000000
VERSION     DASTMD010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2649634"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1092)
                     /locus_tag="VFQ54_09250"
     CDS             complement(145..1092)
                     /locus_tag="VFQ54_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU0165221.1"
                     /translation="MSNPRPFRFGVQIARSRSVSEWRWAAQRAEELGFDVFLMPDHFG
                     PQFAIGPALAMAAAATSTIRIGTLVLQNDLRHPALMVKDFNTLDVLSEGRVELGVGAG
                     GSFVPEYGWTGIPFDAPGPRVSRLEETVQAIKGLSAETPFSFAGKHYTFADYDAFPKP
                     LQQPHVPLLIAGGGNRMLRLAAREADIVGLLPSMKPVGGEFEDENDIAAFAAKAAFVR
                     TEAGERADRLEFNILVQQFQITDGSAAALEQLRADATERGNTDAGWFDSPMVFVGTVE
                     QIAAKLVEVRERVGVSYFTVFEPVMEEFAAVLAHLRATV"
     gene            1223..1549
                     /locus_tag="VFQ54_09255"
     CDS             1223..1549
                     /locus_tag="VFQ54_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0165222.1"
                     /translation="MTAAVPAPIQAMIDATNRGDTKGFLDAFTEDALIDDWGRTFRGR
                     AELASWNETDNIGKQAHFEVRGVEEGDGEYIVTLAVSGNGYNGTGPMHFRLAGDRIAS
                     MIISSN"
     gene            1589..1954
                     /locus_tag="VFQ54_09260"
     CDS             1589..1954
                     /locus_tag="VFQ54_09260"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0165223.1"
                     /translation="MDKINLDEAFARFSESWDPRIVGDINDFQVKVVKLAGAFHWHHH
                     EVEDELFLVHAGRLRMHFRDRVVDLDPGEFIVVPHGVEHMPEALTEECSVLLLEPATT
                     LNTGNVVNERTVADPKRLA"
     gene            complement(1992..2657)
                     /locus_tag="VFQ54_09265"
     CDS             complement(1992..2657)
                     /locus_tag="VFQ54_09265"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEU0165224.1"
                     /translation="MTTDYAGKRAIIIWGGWAGHEPEQTATIFRDVLAGHGFDAALET
                     DLDVFLDADRLAATDLVVPVFTQSEITSDQANGLLTAVREGGTGLGGWHGGMADAFRS
                     HTEYQFATGGQWVAHPGNIIDYTVNITNRDHPITAGIDDFAVHSEQYYLHVDPSNDVL
                     ATTTFTGDYAPWIAGTVMPVVWTRAYGAGKVAYCSLGHVASEFDVPQTKTLVERYLLW
                     ATR"
     gene            complement(2797..>3059)
                     /locus_tag="VFQ54_09270"
     CDS             complement(2797..>3059)
                     /locus_tag="VFQ54_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165225.1"
                     /translation="PFFDAIKVTMAEFQPDIPVIPTLTAGGTDARLLPDIKIYGFFPK
                     LPGVRGEVYGPLVHGHNERVHVDDITFGAEFIWKLVTDFCTK"
BASE COUNT          566 a          962 c         1000 g          531 t
ORIGIN      
        1 taagctgtca tctacgatcg gatacatcca tcaggataag gtcatgcggg ggtgacagtc
       61 ccgccgagcg gtcactcatt tctacgctcg acaccagtcg aggacgcgtc atcggtcgta
      121 gccttgcgtc gcaaggggac gtgactagac cgtggcgcgg aggtgggcga ggacggcggc
      181 gaactcctcc atcaccggtt cgaagaccgt gaagtaggag acgccgacgc gctcccggac
      241 ctcgacgagc ttcgccgcga tctgctccac cgttcccaca aagaccatcg gtgagtcaaa
      301 ccagccagcg tccgtattgc cgcgctccgt ggcgtcggcg cggagttgct ccagcgccgc
      361 ggcgctgcca tccgtgatct ggaactgctg gacgaggatg ttgaactcca gccgatccgc
      421 acgttctccc gcctcggtgc ggacgaatgc cgccttcgcc gcgaaggcgg cgatatcgtt
      481 ctcatcctcg aactcgccgc cgaccggctt catgctcggc agcaggccga cgatgtccgc
      541 ctcccgcgcc gcgagacgca gcatccggtt gccaccaccg gcgatcagca gcggcacgtg
      601 cggttgctgg agcggtttcg ggaaggcgtc gtagtcggcg aacgtgtagt gctttccggc
      661 gaacgagaag ggcgtctcgg cggaaagacc cttgatcgcc tggaccgtct cctcgaggcg
      721 ggaaacgcgc gggcccggcg cgtcgaaggg aatccccgtc cagccgtact ccggcacgaa
      781 cgaaccaccg gctccgaccc cgagctcgac gcggccctcg gagaggacat cgagcgtgtt
      841 gaaatccttc accatcagcg cgggatgacg gagatcgttc tgaaggacga gcgtcccgat
      901 ccggatcgtg ctcgtcgcgg cggcggccat cgccagcgcc ggtccgatcg cgaactgcgg
      961 cccgaagtga tcgggcatca ggaagacatc gaagccgagt tcctcggcgc gctgggccgc
     1021 ccaacgccat tcggagacgc tgcgcgaccg ggcgatctga acgccgaagc ggaatggtcg
     1081 gggattgctc atgcggttct ccatctggtg tgaatcagtg cgaagctgcg acgatcatcg
     1141 cacgagcggc gattctcccc aaggccgcgc gagccgtacg attgggaagg atacactcgt
     1201 cgatgcaagg agagatcacg ccatgaccgc agctgttccc gcgccaatcc aggcaatgat
     1261 cgacgccacc aatcgtggcg ataccaaagg attcctcgat gccttcaccg aagacgcact
     1321 gatcgacgac tggggccgca cgttccgcgg ccgggcagaa ctcgcgagct ggaacgagac
     1381 cgacaacatc gggaagcagg cgcatttcga agtgcgcggc gtcgaggagg gggacggcga
     1441 atacatcgtg acgctcgccg tctcggggaa cggctacaac ggcaccggtc cgatgcactt
     1501 ccgtctcgcg ggcgacagga tcgcgagcat gattatcagt tcgaactgat cgccacgcgc
     1561 gcgattcatt ccgtgagggg aggacgcaat ggacaagatc aatctcgatg aggcgtttgc
     1621 acggttcagc gagtcatggg acccaagaat cgtcggcgat atcaacgact ttcaggtcaa
     1681 ggtcgtgaag cttgccggtg cgtttcactg gcatcatcac gaggtggagg atgagctttt
     1741 cctcgttcac gccgggcggc tgcgcatgca cttccgagac agggtcgttg atctcgatcc
     1801 cggggagttc atcgtcgtgc cgcacggcgt cgagcacatg cccgaagcgc tgaccgagga
     1861 atgctccgtg ctgctgctcg aaccggcaac gacgctcaac accgggaatg tcgtcaatga
     1921 gcggacggtg gccgacccga agcgcctcgc atagccggat agggcggggt tttccgcccc
     1981 acaggcgtct cctaccgcgt cgcccagagc aggtagcgct cgaccagcgt cttcgtctgt
     2041 gggacgtcga actccgatgc gacgtgaccg agcgagcagt aggcgacctt tcccgcgccg
     2101 taggcgcgcg tccagaccac cggcatcacc gtcccggcga tccacggcgc gtaatcaccg
     2161 gtgaaggtcg tcgtggcgag cacgtcgttc gaggggtcga catgcaggta gtactgctcg
     2221 gagtggaccg cgaaatcgtc gattcccgcc gtgatcgggt ggtcgcgatt cgtgatgttc
     2281 accgtgtaat cgatgatgtt ccccggatgc gcgacccact ggccacccgt cgcgaactga
     2341 tactcggtgt gggagcggaa ggcgtccgcc atgccgccgt gccatcctcc gagaccggtt
     2401 cctccctcgc gaaccgccgt cagcaggccg ttcgcctggt cggacgtgat ctcgctctgg
     2461 gtgaagaccg gcacgacgag atcggtcgcg gcaaggcggt cggcatcgag gaagacgtcg
     2521 agatccgtct cgagggcggc gtcgaagccg tgtccggcaa ggacatcgcg gaagatcgtc
     2581 gccgtctgct cgggctcgtg gccggcccag ccgccccaga tgatgatcgc gcgcttgcct
     2641 gcgtagtcgg tggtcacgag gtagctccct tgacgttcgt ggggtgaatc catggttcgg
     2701 acgcgagcat atccgatgcc gccggatttc gcgaccgcca cccgttcccc tctcgacgcc
     2761 gacgatcgat ggccggcgtc tgtcttcggc acatccctat ttcgtgcaga agtcggtgac
     2821 gagcttccag atgaactcgg cgccgaaggt gatgtcatcg acgtggacgc gctcgttatg
     2881 tccatgcacc agcggcccgt agacttcgcc acgtacaccg ggcagcttcg ggaagaagcc
     2941 gtagatcttg atatccggca gcagacgggc gtcggttccg ccagcggtaa gcgtcgggat
     3001 gacggggatg tcgggctgga actcggccat cgtcaccttg atcgcatcga agaagggcg
//
LOCUS       DASTMD010000628         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1488905, whole genome shotgun sequence.
ACCESSION   DASTMD010000628 DASTMD010000000
VERSION     DASTMD010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488905"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFQ54_09275"
     CDS             <1..394
                     /locus_tag="VFQ54_09275"
                     /inference="COORDINATES: protein motif:HMM:NF036579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0165226.1"
                     /translation="FNHYSLGSVGEWLRKSVAGIDNVPGTSGYKKIRIKPHLDAAMTF
                     AEGSYDSIRGLIRSRLERSDGGTIVEVEIPANATAEIWLPASSVESVTEGAASVSQAA
                     GVTAVRVDGDRVIVSVGSGSYRFVVTSS"
     gene            858..1406
                     /gene="tpx"
                     /locus_tag="VFQ54_09280"
     CDS             858..1406
                     /gene="tpx"
                     /locus_tag="VFQ54_09280"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU0165227.1"
                     /translation="MALANERLGEAFELGEQLTVVGSRLVPGSSAPDFALERFDLAVE
                     AMSTVTLADSAGTVRLLNVINSLDTPVCNIETRRWDQLRGDLPDGVTLYTISMDLPFA
                     QARWIGAEGAEHQALSAHKNEAFGVDYGVLLKEWRLLQRAVFVIDANDTVTYVEYVHD
                     QMREPNYDAAVAAVRAAAGHDA"
     gene            1574..2035
                     /locus_tag="VFQ54_09285"
     CDS             1574..2035
                     /locus_tag="VFQ54_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165228.1"
                     /translation="MAFAWTDVSGFAAHSTYEQDFLLDNYDASPLGPGTYLDRSRRLD
                     QTPKLISWSSNLPAAYLDTRLGDSDDEVAYTIGSGNAAAIEPGVLYTIRIEAKPGDAG
                     RDSARLSAQLGHQDPPGCELTWCSFGDASVEIFPAWTIPVPGRIAIGAPPR"
     gene            2135..>2411
                     /locus_tag="VFQ54_09290"
     CDS             2135..>2411
                     /locus_tag="VFQ54_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165229.1"
                     /translation="MSVLTVRFASKALGKQTGFNVIHPDRGDGPFPVLMQLHGYSDDS
                     FSWLYNSSIVRHVADLPLIVVLPDGGTSRYMNLSRHPRFGLARYEDLL"
BASE COUNT          415 a          770 c          761 g          465 t
ORIGIN      
        1 gttcaatcac tactcgttgg gttcggtcgg cgaatggctg cggaagtccg ttgcaggaat
       61 cgacaatgtg cccgggacgt ccgggtacaa gaagatccgg atcaagccgc atctcgacgc
      121 ggcgatgacc tttgccgaag gcagctacga cagcattcgt gggctgatcc ggagtcgctt
      181 ggagcgttcg gatggaggga cgatcgtcga agtcgagatt cccgcgaacg cgacggccga
      241 aatctggctc cccgcatcat ccgttgaatc agtcacagaa ggcgccgcgt cggtctccca
      301 ggcggcgggc gtgacagccg ttcgcgttga cggcgaccgc gttatcgtca gtgtgggatc
      361 cggatcctac cgctttgtgg tgaccagttc ctgagagccc tctctccgaa cgaacgttct
      421 gcggctagag ataaactcta gccctacaaa ccccggcctt tttccatcga gtcgatcgca
      481 attgcgtcgc attccggaac catcacccac tccgtgcgca tgcgccgggg aatggtgatt
      541 ccgacgttca tgagtaggga cgctctctcg tcgtcggcca gtagggaaat gagctggccc
      601 gcctccccgt tgccgaaccg ggctcgccct acacgccacc cccggtcagg tggcttccgc
      661 tccggattct gctctggcat ccgtcacaat ccgtggcctc ttcccttttg cggtctaccc
      721 gaattgcgcc ccgggcgccg gtgacccacg atcaccggcg cgggaaggaa gatgccatac
      781 tgcctgatct ggacgatgct gtccgtctgg ccagcaggag aatcaaggcg gcggttccgc
      841 cgaactggag caggtacatg gcattggcga acgagcgttt gggtgaggct ttcgagcttg
      901 gcgaacagct gaccgtcgtc ggcagtcgtc tcgtgccggg cagctccgcc ccggatttcg
      961 cgctcgagcg attcgacctc gccgtcgagg cgatgtcgac cgtaacactg gccgactcgg
     1021 cggggacggt ccggctgctc aatgtcatca attccctcga cactcccgtc tgcaacatcg
     1081 agacgcgacg ctgggatcag cttcgtggcg atcttcccga cggcgtgacg ctctacacga
     1141 tcagcatgga ccttccgttt gcccaggcac gatggattgg cgccgagggc gcggagcacc
     1201 aggcgctatc cgcccacaag aacgaagcgt tcggcgtcga ctacggcgtc ctgctcaagg
     1261 agtggcgatt gctccagcgt gcggtcttcg tgatcgacgc gaacgacacg gtcacctacg
     1321 tcgagtacgt ccacgaccag atgcgcgagc cgaattacga cgcggcggtc gccgccgtgc
     1381 gcgccgcagc cgggcacgac gcttgaggtc gtgtcgagtc ggaccgcgat cgatcgcggt
     1441 cgcgcttctg ctcgttctgg catggacgcc cgcgatcgca cgggcatcgg gggttcccga
     1501 tcccgccacg tgggttcccg gctacgtcag cgtttcctcc ggacagaacc cggacggaac
     1561 acgatacgtg gagatggcct tcgcatggac ggatgtctcc gggttcgcgg cgcactcgac
     1621 gtacgagcag gatttcctcc tcgacaacta cgacgcgtct ccgctcggtc cgggaaccta
     1681 tctcgatcga tcgcgtcgct tggatcagac gccgaagctg atctcctggt cgtcgaatct
     1741 gccagcggca tacctcgata cccggctcgg cgacagcgat gacgaggtgg cctatacgat
     1801 cggatcggga aacgcggccg cgatcgagcc cggcgttctc tacacgatcc ggatcgaagc
     1861 gaagccaggg gatgccggtc gcgacagtgc gcggttgtcg gcgcaactcg ggcatcagga
     1921 cccgcccggg tgcgaactga cctggtgcag cttcggcgac gcgtcggtcg agatcttccc
     1981 ggcctggacg attccggtgc cgggacggat cgccatcggc gctcctccgc gatagcatgg
     2041 ctccgagaag acgcggcgat atttcgatgc gtgcagcatg ttcgtcggca gcgggcggtc
     2101 gcggcagcga cgctcgccgc ctgggggtcc tgaaatgagt gtgttgacgg tccggttcgc
     2161 gagcaaggcg ctcggcaagc agacgggttt caatgtgatc catcccgatc gcggggatgg
     2221 cccgtttccc gtgctcatgc aactacacgg ctatagtgac gacagttttt cctggctcta
     2281 caactcgagc atcgtccgtc acgtcgccga tcttccactc atcgtcgtcc tgccggatgg
     2341 gggaacgtcg cggtacatga acctctcgcg tcatccccgg ttcggtctcg ctcgctacga
     2401 ggacctcctg a
//
LOCUS       DASTMD010000629         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1309275, whole genome shotgun sequence.
ACCESSION   DASTMD010000629 DASTMD010000000
VERSION     DASTMD010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309275"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFQ54_09295"
     CDS             complement(<1..587)
                     /locus_tag="VFQ54_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEU0165230.1"
                     /translation="MSTETHSAPGASAQSGSTTRFTSFWLYQRKENWDDITGDAWDAA
                     TSQLTSMLNELPDDVVLRGSYSSLGLTPDVELILWAVAPKLEQLQRLAIGVDRTTIGH
                     YLRLQKIYFGSAGISQYDPTHGPAFLLGKDPKRYLSVYPFTKTPDWFLLPYEERRRLM
                     IEHGQMGRDYPSILTNTVNSFGVADQEFIVALEDD"
     gene            complement(1005..1907)
                     /locus_tag="VFQ54_09300"
     CDS             complement(1005..1907)
                     /locus_tag="VFQ54_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165231.1"
                     /translation="MIATTTSSATPLPNIARRTVRTEALGFAKRLFRYRQAQIAGFII
                     LVLVLAAIFAPLIAPYAPDAIGAGRPLTPPNRHYIFGTDDLGRDMFSRMLYGARITLR
                     IGAIIVAVELVIGVTIGLVSGYFGGWVDMILMRFTDVMLAFPGIMLALAIVSTLGPGL
                     TNAIFAVALGAWPVFARVVRSSVLTVRESEYVLAGRVLGATNMHMMVRGILPNVIAPI
                     IVLASLEFPAAVLVAAALSFLGLGAQPPAPEWGALLTDARNYIRSAPYLINIPGLAIF
                     CTVLAFNLLGNALRDVLDPRLRQR"
     gene            complement(1904..>2110)
                     /locus_tag="VFQ54_09305"
     CDS             complement(1904..>2110)
                     /locus_tag="VFQ54_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0165232.1"
                     /translation="FGGLLSGAFIIEAVFAWHGIGELGVQAFAKRDFPLIQGVVLVVA
                     TSYVLVNLVVDILYAFLDPRVKLS"
BASE COUNT          429 a          657 c          691 g          333 t
ORIGIN      
        1 tcgtcatcct cgagcgccac gatgaactcc tggtcggcga cgccgaacga gttgaccgtg
       61 ttcgtgagga tgctcgggta gtcgcgcccc atctgaccgt gctcgatcat caggcgacgg
      121 cgctcttcat atggaagaag gaaccagtcc ggcgtcttgg taaagggata gacgctgaga
      181 tagcgcttcg gatccttgcc gaggaggaag gccggtccgt gggtcggatc gtactgcgag
      241 atccccgccg aaccgaagta gatcttctgg agacgcaggt aatggccgat cgtcgtccgg
      301 tcgacgccga tcgcgagccg ctgcagctgc tcgagtttcg gcgcgaccgc ccagaggatg
      361 agctcgacat ccggggtcag gccgagggac gagtaggagc cgcgaaggac gacatcgtcc
      421 ggcagctcgt tcagcatcga ggtcaactgg ctggtcgcgg catcccaggc gtcgccggtg
      481 atgtcgtccc agttttcctt gcgctggtag agccagaagg aggtaaagcg agttgtcgac
      541 ccggattggg ccgaggcgcc cggtgcggaa tgggtctcgg tagacatgac ggacgatctc
      601 ccctgcgagg tgcgcggaat gcacaatcga ttcgccccaa cggtcacacg aatcgaccgt
      661 gcattcaagg aagatcacgc atcgttgcca gttggtctgc ctcggatcag acaatcttgg
      721 cagcgttcgc cggggtccga gagtccacca gatgagaggc ggagacgggg cgcccggtga
      781 gaggtcatcc atggaaaacc gggcgaacct ggtgttcgcc cggcgggcgg gacgcacccc
      841 gcatgcagat cccggcggcc tacctgccac ccagacgtcc acgcagaaca tcttgtccac
      901 aacgttcata cgaacagcgg ttgtgagtac ggcgggactt ctatgtcccg ccttcatcgg
      961 cgatcgttac cttcgtcggg acgcagcccg gacgtcgaca ccccctaccg ttggcgcagg
     1021 cgcgggtcga ggacatcgcg caacgcgttg ccgagcaggt tgaaggcgag caccgtgcag
     1081 aagatcgcca gccccgggat gttgatcagg tacggcgccg accggatgta gttccgggcg
     1141 tctgtgagaa gcgcccccca ttccggcgcc ggcggctgcg cgccaagacc caggaacgag
     1201 agcgccgccg ccacgaggac cgccgccggg aactcgagcg acgcgagcac gatgatcggc
     1261 gcgatcacat tggggagaat cccgcgcacc atcatgtgca tgttggtcgc tcccagcacc
     1321 cgtcccgcga gcacgtattc cgattcccgc acggtcagaa ccgaactcct gaccacccgc
     1381 gcgaagaccg gccacgcgcc gagcgcgacc gcgaagatcg cgttcgtcag gccaggtcca
     1441 agtgtcgaga cgatcgccag cgcgagcatg atcccgggga acgcgagcat cacatccgtg
     1501 aagcgcatca ggatcatgtc gacccagcca ccgaagtacc cggagacgag gccgatcgtc
     1561 acgccgatca cgagctcgac cgcgacgatg attgcaccga tccggagcgt aattcgcgcg
     1621 ccgtagagca tgcggctgaa catgtcgcgc ccgaggtcat ccgtgccgaa gatgtagtga
     1681 cggttgggtg gcgtcagcgg tcgtcccgct ccgatcgcat ccggtgcata tggcgcgatc
     1741 aatggcgcga agatcgcggc gagcaccagc acaaggatga tgaatcccgc aatctgtgcc
     1801 tgccggtaac gaaagaggcg cttcgcgaaa cccagggcct ccgtacggac cgtgcgccgg
     1861 gcaatgttcg gcaggggtgt tgccgatgat gtggtcgtcg caatcatgag agcttcaccc
     1921 gcgggtcgag gaacgcgtag aggatgtcca ccacgagatt gacgagcaca tacgaggtcg
     1981 cgacgacgag gacgacgccc tggatcaggg gaaagtcacg cttcgcaaac gcctgcacgc
     2041 cgagctcccc gattccatgc caggcgaaga ccgcctcgat gatgaaagcg ccgctgagaa
     2101 gcccgccgaa
//
LOCUS       DASTMD010000630         1724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1846188, whole genome shotgun sequence.
ACCESSION   DASTMD010000630 DASTMD010000000
VERSION     DASTMD010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846188"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VFQ54_09310"
     CDS             <1..806
                     /locus_tag="VFQ54_09310"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0165233.1"
                     /translation="LFIATYLLIDVPKFKETFVNAFAHKYRPDAEQLWRTLGDSLSRY
                     LAGLLISITFQGISAMIGLYIIGIPYALILGIWMSATAILPYIGAFLGAIPAVLLALS
                     IGWETTVLTVILYVVVNQIDGNFVTPRVQGTAVRVHPVLIFVSVIGGSEILGPLGAIL
                     AVPTLAVIRVLAEFFWVRLRVNDEQQEPLLVAMSGSELDIVDDDLEVKVDLDPDDEPA
                     TVTVTDRQDPESDDTTTEITVSRGDDDTPSANGESGRQIVAKSRDSGDP"
     gene            complement(1208..>1724)
                     /locus_tag="VFQ54_09315"
     CDS             complement(1208..>1724)
                     /locus_tag="VFQ54_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU0165234.1"
                     /translation="PVPADAWAVAAFAGYIPEQERTEQQRDVLALAAELTDELVAADA
                     LLLAVPLYNYGVSQHFKTWVDLILTDPRMNPGGEPAVAGKPAVLVTVRGGSYQAGTPR
                     EGWDHATAWMQRILADVWKLDLKIVESEFTMVGVNPAMDRFKEDAARVRAAAEELART
                     HGRALTAVGVA"
BASE COUNT          298 a          577 c          546 g          303 t
ORIGIN      
        1 tcctcttcat cgccacctat ctcctgatcg acgtcccgaa gttcaaggag accttcgtca
       61 acgccttcgc ccacaagtac cggccggacg ccgaacaact ctggcgcacg ctcggcgatt
      121 cgctctcccg ctatctcgcc gggctgctga tctcgatcac cttccagggt atatcggcga
      181 tgatcggtct ctacatcatc ggcattccct acgccctgat cctcgggatc tggatgtcgg
      241 caacggcaat ccttccgtat atcggcgcct tcctcggcgc gattccggcg gtgctccttg
      301 ccctctcgat cggctgggag acgaccgttc tcacggtcat cctctacgtg gtcgtcaatc
      361 agatcgacgg caacttcgtg acgccacgcg tacaggggac cgcggtccgc gtccacccgg
      421 tcctcatctt cgtctcggtg atcgggggga gtgagattct cggaccactg ggcgcgatcc
      481 tcgcggtccc gacgctggcc gtgattcgcg tgctcgccga gttcttctgg gtccggctcc
      541 gggtaaacga cgagcagcag gaaccgctgc tcgtcgcgat gtccggcagc gaactggaca
      601 tcgtcgacga cgacctggag gtcaaggtcg atctcgaccc cgacgacgaa ccagcgacgg
      661 tgactgttac cgatcgtcag gatccggaat ccgacgacac gacgacggag atcacggtct
      721 ccagagggga cgacgacacc ccgtccgcca atggcgaatc ggggcgtcag atcgtcgcga
      781 agagccggga ttcgggagac ccctgatcga gcctgagagg cggatgccac aacctggcgg
      841 atgcatcagt acatgcggcg tccaccaagt cacgaccggt gtgatgagaa caggggcgta
      901 cttcgcccga gtccgctttg cggcggcatt gggtatgaaa ccccgcccta ccaaccccag
      961 caccagcaaa acgggtcgac ggttctggat ttcgatgtag ggaatcgcgt atcggatcag
     1021 cgtccttgag tagggcggga catagaagtc ccgccttccg gggcggcaca atccgggacg
     1081 cagctcggga ccatcggtcg ttcgtagggc gggaccgccg ctgccggacc ggaggtccgg
     1141 acggcctgtc ccgcctcccg cctgccccgc cggtttcatg ctggaggaat gcggcgtggc
     1201 cgatagctca agcgacgccg accgcggtga gggcgcgacc gtgggtgcgc gccagttcct
     1261 ccgccgcggc gcgaacgcgg gcggcgtcct ccttgaagcg atccatcgcc ggattgacgc
     1321 ccaccatcgt gaactcgctc tcgacgatct tgagatcgag cttccagaca tcggccagga
     1381 tgcgttgcat ccaggcggtc gcgtgatccc acccctcgcg cggggtacca gcctggtagc
     1441 tgccgccacg gacagtgacc agcaccgccg gctttccggc gacggccggc tcaccgcccg
     1501 ggttcatccg cggatccgtg aggatgaggt cgacccaggt cttgaagtgc tgggaaacgc
     1561 catagttgta gaggggaacc gcgaggagca gcgcatcggc ggcgaccagc tcatcggtca
     1621 gctccgctgc gagcgcaagc acgtcacgct gctgttccgt tcgctcctgc tcggggatgt
     1681 agccggcgaa cgccgcgacg gcccaggcgt cggccggaac cggc
//
LOCUS       DASTMD010000631         1596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1576866, whole genome shotgun sequence.
ACCESSION   DASTMD010000631 DASTMD010000000
VERSION     DASTMD010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576866"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VFQ54_09320"
     CDS             <1..676
                     /locus_tag="VFQ54_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU0165235.1"
                     /translation="ASSEETVRVSQAMEDYLKAAYRLEQEGGPVSTQRLADELGISGP
                     SVTNMVKRLDELKLLKHSRYHGVTLTPSGQHVALEVIRHHRLLELYLAEALGFDWDEV
                     HAEAERLEHHVSEELERRMEKALGYPQFDPHGDPIPSRTGTFPEVDDIALPDVPAGKS
                     AQVSRVSDRDPDHLRHLASLGVKPGVTLDVIEHLPFDGPIRVTVDGVEQLLSLTVARG
                     VRLAHD"
     gene            complement(752..1462)
                     /locus_tag="VFQ54_09325"
     CDS             complement(752..1462)
                     /locus_tag="VFQ54_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165236.1"
                     /translation="MVLFLKLIWRGFRDLFERLLQFVSYSVLWWLAIITIVGGPPATV
                     ALFWICDPRNRVSTPEFADAVAVFKGSIRRSWGITIFTVPIFAILFWNTFFFSGSNHP
                     LVALVPLWILLLFVLYIVILYAFSMAATMESGTRNAFRGAMFVLISHPFRSIFLCLFL
                     IVLGTVMTVTVLPLVLFGPALLAAIVNRFVLDALKIDVDDPDAPTIERQAERQHGINP
                     DPTLLNRLRGSNKQKRQG"
BASE COUNT          346 a          492 c          507 g          251 t
ORIGIN      
        1 agcgtcatcc gaagaaaccg tgcgcgtcag ccaggcgatg gaggactacc tcaaggccgc
       61 ctaccggctc gagcaggaag gcggtcccgt gagcacgcag cgacttgccg acgaactcgg
      121 gatcagcggt ccgtccgtca cgaacatggt gaagcggctc gatgagctca agctcctcaa
      181 gcacagccgc taccatgggg tgacgctgac accgtccggg caacatgtcg cgctcgaagt
      241 gatccgtcac caccgcctcc tcgagctcta cctcgccgag gcgctcggct tcgactggga
      301 tgaggtgcat gccgaggcgg aacgtctcga gcatcacgtc tcggaggagc tcgagcggcg
      361 aatggagaag gcgctcgggt acccccagtt cgatccgcac ggcgacccga tccctagccg
      421 cacgggaacc ttccccgagg tcgacgacat cgccctgccc gacgttcccg ccgggaagtc
      481 cgcccaggtg agtcgtgtca gcgaccgcga tcctgatcat ctccggcatc tggcgagtct
      541 cggcgtgaag ccgggcgtca cgctcgacgt gatcgagcat ctgccgttcg acggcccgat
      601 ccgggtcacc gtcgacgggg tcgaacagct cctgtcgctg accgtcgcgc gcggggtacg
      661 tctggcccac gactagacat tccgtcagtc agatcgaaaa gggcggcttc cccgggggag
      721 ccgccctttc gtcgtttcga tgggtctggc gtcacccctg ccgcttctgc ttgttgctgc
      781 cgcgaagacg gttcagaagc gtgggatcgg gattgattcc atgctggcgc tccgcctggc
      841 gctcgatcgt cggcgcgtcc gggtcgtcaa catcgatctt caaggcgtcc aggacgaatc
      901 gattgacgat cgcagccagc agcgccggcc cgaagagcac gagcggcagg acggtcaccg
      961 tcatgaccgt tccaagcacg atcaggaaga ggcagaggaa aatcgatcgg aacgggtgac
     1021 tgatgagaac gaacatcgcg ccacgaaagg cgtttcgcgt tcccgactcc atcgtcgctg
     1081 ccatcgagaa ggcgtagagg atgacgatgt agagaacgaa aagcaggaga atccatagtg
     1141 gaaccagggc gacgagcgga tgattcgagc cagaaaagaa gaacgtattc cagaagagga
     1201 tcgcgaaaat ggggacggtg aagatcgtga ttccccacga ccgccggatc gatcccttga
     1261 agactgcgac cgcgtcggcg aattccggcg tcgagacgcg attacgcgga tcgcagatcc
     1321 agaagagcgc gaccgtcgcc ggcggccccc ccacgatcgt gatgatggcg agccaccaga
     1381 gcacagagta ggacacgaac tgaagcaggc gctcgaaaag atcgcgaaat ccccgccaga
     1441 tcaacttgag aaaaagcacc aacctgcacc cacactcgtc atcgcggcga tttcggtcat
     1501 tgcggagcac acaatgccga tgattgtacg cgaacgtcgg cacgggcatc ggtgcattcg
     1561 cgacatcgcg ggcgagaagg cgagggggct gctata
//
LOCUS       DASTMD010000632         1805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1755319, whole genome shotgun sequence.
ACCESSION   DASTMD010000632 DASTMD010000000
VERSION     DASTMD010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755319"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /gene="ligA"
                     /locus_tag="VFQ54_09330"
     CDS             <1..1248
                     /gene="ligA"
                     /locus_tag="VFQ54_09330"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU0165237.1"
                     /translation="IDEVWERSQWWLERRHSLDFEIDGMVIKVDNLDLQEEIGYVARD
                     PRWATAYKFPAIQQTTRVLDILINVGRTGTLNPLAVLEPVNIGGVTVSRATLHNEEEI
                     ARKDIRIGDTVVVQRAGDVIPQIVKVVEERRTGDEVPFQMPDVCPVCGTPVVKEPGVV
                     MRYCTNASCPAQLKERMHHFVSRAAMDIEGLGAKLADRFIDLGWIHDASDIYHLDWEQ
                     VAQLEGLGEKSAENLKASIEKSKAQPMWRLLHGLGIRHVGERTATLIADRFGSFEALF
                     QATEEDIEEIAGIGSIVAESVYDFAQEPRNRELIQRLAAAGVTVEDVRDHAHGERPLD
                     GLTLVLTGRLETLTRPEAEERLRQAGANVTSSVSKKTSAVIAGADAGSKAEKAISLKV
                     PVLSEDDLQTLLQGVLPDALKEN"
     gene            1310..1567
                     /locus_tag="VFQ54_09335"
     CDS             1310..1567
                     /locus_tag="VFQ54_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165238.1"
                     /translation="MHRPVAKPWNKEQPMSGYTISSREDDPVHTVRTIARISQMLIEL
                     RDEYVSRPREDTLQQIEQRLGDLIALRDELKVKIAHQRERE"
BASE COUNT          361 a          591 c          570 g          283 t
ORIGIN      
        1 atcgacgaag tctgggaacg gagccagtgg tggctcgaac gacgccactc gctcgatttc
       61 gagatcgacg gcatggtgat caaggtcgac aatctcgatc tgcaggagga gatcggctac
      121 gtcgcgcgcg atccccgctg ggccacggcc tacaagttcc cggcgattca gcagacaacc
      181 cgcgtgctcg acatcctgat caacgtggga cgcaccggga ccctcaatcc gctggcagtg
      241 ctcgagccgg tcaacatcgg tggcgtcacc gtgagccgcg cgaccctgca caacgaggag
      301 gagatcgccc gcaaggacat ccggatcggc gacaccgtcg tcgtgcagcg cgccggcgac
      361 gtcatccccc agatcgtcaa ggtcgtcgag gagcgacgga ccggcgacga ggttcccttc
      421 cagatgccgg acgtctgccc ggtctgcgga acgcccgtcg tcaaggagcc gggcgtcgtc
      481 atgcgctatt gcacgaacgc ctcctgcccc gcccagctga aggagcgcat gcatcacttc
      541 gtcagccgcg cggcgatgga tatcgagggc ctcggcgcca agctcgcgga tcgcttcatc
      601 gatctcggct ggatccacga cgccagcgac atctatcacc tcgactggga acaggtcgcg
      661 cagctggagg ggctcggcga gaagagcgcc gagaacctga aggcgtcgat cgagaagagc
      721 aaggcgcaac cgatgtggcg cctgctccac ggcctcggga tccgccacgt tggcgagcga
      781 accgcgacct tgatcgcgga ccgtttcgga tccttcgagg cactcttcca ggcgaccgag
      841 gaggatatcg aggagatcgc cgggatcggc agcatcgtcg cggagagcgt ctacgacttc
      901 gcccaggaac cacgcaaccg cgagctcatc cagcgcctcg ccgcggccgg tgtcaccgtc
      961 gaggatgttc gcgaccatgc gcacggcgaa cgcccgctcg acggtctcac gctggttctc
     1021 accgggcggc tcgaaacgct gacgcgaccg gaggcggagg aacgactacg ccaggcgggc
     1081 gcgaacgtca cgagttcggt ctcgaagaag acgtccgcgg tgatcgccgg cgccgatgcg
     1141 gggtcgaagg ccgagaaggc aatctcgctc aaagtgcctg ttctctccga ggatgacctg
     1201 cagacgctgc ttcagggcgt tctccctgat gcgttgaagg aaaattgatg cgtacgctgg
     1261 catcgtccgt gatacgctat ccaacgggtc ctggaggtcg gctgcacgga tgcatcgacc
     1321 ggtcgcgaag ccgtggaaca aggagcagcc gatgagcggc tatacgattt cttcccggga
     1381 agatgatcct gtgcacacgg tgcgcacgat cgcccggatc tcgcaaatgc tgatcgaact
     1441 gcgcgatgag tacgtttcac gaccgcgtga ggacacactc cagcagatcg agcagcgcct
     1501 tggcgacctc atcgcgcttc gcgatgaact caaggtcaag atcgcccatc agcgtgagcg
     1561 cgagtaacca tccacccgtg ctggaggctg aaaccaccct ccagccgtga gccgcgtcaa
     1621 gggccgacaa gggcctacgc tatgatcgtc gtccccggtt tcaccagcgg aataccgttc
     1681 gcgcagagcg ggcattcatc cgcgctccag gacgcgacaa cctgggaacc gagcgcatgg
     1741 agcgggatct ccccgaactg aaccttcccg ccgctccggt cgaccagaac cccgatcccc
     1801 actac
//
LOCUS       DASTMD010000633         3235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1667790, whole genome shotgun sequence.
ACCESSION   DASTMD010000633 DASTMD010000000
VERSION     DASTMD010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667790"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2643
                     /locus_tag="VFQ54_09340"
     CDS             <1..2643
                     /locus_tag="VFQ54_09340"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0165239.1"
                     /translation="GLLTGDVAENRNARIIVMTTEILRNMLLQSPWELDDVDCVIFDE
                     IHYLADPERGTTWEESIILCPEHIQFICLSATVTNADEIAGWIGRTHRPIQLITHHRR
                     PVPLSLYYFYEDELSQVVDHTGKQVKSFNKIGGELRRQPGRAGFRRQADRSSQAMEEP
                     QAREIIDALASQNLLPAIYFLFSRNDCQAYAERLAMMRPELVTPAQETSIEAILQTYL
                     NSMRPEDTDLEQVQIITRLARRGIGFHHAGLLPVLKQLVEVLFSRGLMKVVFATDTLA
                     LGVNMPARTVVIGRLTKWDGRRRRALIPNEFQQMAGRAGRRGMDAFGHVVIPYSPWIP
                     YKEVLEIATGALHPVQSAFAIRYNTVLNLWDPPTGERVRQMLQRSLAQYQTSQRIRLM
                     ENDVLEIDAELAGLIDGNAIEIGQNDEAYEQYQSLNRATQALQQQVKRSEQELADLML
                     SSGQATPWTTPGRQALRRVLRSAPVGTVVHTQDHGWAVYVGRGASGGVGVFLCQRDNR
                     IRSLTEYRKIDNLPDDVVVSIPEALIEPPELVSDATELVPADELARIWNDLNDLGLPD
                     LDKLANEHRAAQAERIASESERIQGEIAAAQEEISQLRVEMKEHPFHDTEWRKQYQRF
                     LRRRDNLEKERNALNDILDREIEAEDVRIRNVIQGIRDVLHRFGYMRRGYPTEKADML
                     ADVFDTDGLILCELVDRGMLEPLAPEELAELFSWYSFDRDFRYANHFTLPERLVNVRR
                     RLEELERDVLGEERDHKLFISEGHNPSFYGAALHWSRGWSMVQIGEQMELSEGDLVLT
                     FNKTIDLMRQVRDMLMDVQPEHPLIPNLHRAERLLRRGIVEQSLTLGFSPIADAFEAE
                     AEDQPVDGPADDPDTDEDAATAIV"
     gene            2679..>3235
                     /locus_tag="VFQ54_09345"
     CDS             2679..>3235
                     /locus_tag="VFQ54_09345"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEU0165240.1"
                     /translation="MHRTDRLTGILLALQAGPRSASELAARFEVSKRTIMRDVDALDQ
                     IGVPVIAETGRNGGYRIADGYWMPPLQLTADEATVLLFALQHVGSVDRSPLGDAHRTV
                     LEKLQAHLTPGIAPDVVKNLDTMVVLRDHDAPDAAIIALLREAVAAECWIAIVYAGGS
                     GPSERSILPLRVYTSGGRWYADAVD"
BASE COUNT          607 a         1102 c          983 g          543 t
ORIGIN      
        1 ggtctcctca ccggcgacgt cgcggagaat cgcaatgcgc ggatcatcgt gatgacgacc
       61 gagatcctgc gcaacatgct cctccagtca ccgtgggaac tcgacgatgt cgactgcgtg
      121 atttttgacg agatccacta cctcgccgat ccggagcgcg gcacgacctg ggaagagtcg
      181 atcatcctct gccccgagca tatccagttc atctgcctct ccgcgacggt gacgaatgcc
      241 gacgagatcg ccggctggat cggtcgcacg caccggccga tccagctgat cacgcatcat
      301 cgccgaccgg ttcccctttc gctctattac ttctacgagg atgaactgag ccaggtcgtc
      361 gatcacaccg gcaagcaggt caagagcttc aacaagatcg gcggcgagct tcgccggcag
      421 ccgggacgcg ccggattccg gcgtcaggcc gatcgctcgt cccaggcgat ggaggaaccg
      481 caggcgcgcg agatcatcga tgcgctcgcg agtcagaacc tgctccccgc gatctatttc
      541 ctcttcagtc gcaacgactg ccaggcctac gccgaacgcc tcgcgatgat gcgacccgaa
      601 ctcgtgacgc ccgcccagga gacgagtatc gaggcgatcc tccagaccta tctcaacagc
      661 atgcgaccgg aggataccga cctcgaacag gtccagatca tcacccgcct cgctcgacgt
      721 ggcatcggct ttcaccacgc cggtctgctg ccggtcctca agcagctcgt cgaggtcctc
      781 ttcagccgcg gcctgatgaa ggtcgtcttc gcgacggata cgctcgccct cggtgtcaac
      841 atgccagccc gtaccgtcgt gatcggacgc ctgacgaagt gggacgggcg acggcgacgg
      901 gcattgattc cgaacgagtt ccagcagatg gcaggtcgcg ccggtcgccg cggcatggat
      961 gccttcggtc atgtcgtgat cccgtactcg ccgtggattc cctacaagga agtactggag
     1021 atcgcgaccg gcgccctgca tcccgtgcag tcggcattcg cgatccgcta caacacggtg
     1081 ctcaacctct gggatccccc gaccggcgaa cgcgtgcgcc agatgctgca gcggagtctc
     1141 gcgcagtacc agacgagcca gcgcatccgg ctgatggaga acgatgtcct cgagatcgac
     1201 gccgagctcg ccggattgat cgacggcaac gcgatcgaaa tcggccagaa cgacgaggcg
     1261 tacgagcagt accagagcct gaaccgcgcc acccaggccc tccagcagca ggtgaagcgg
     1321 tcggaacagg aactcgccga cctcatgctc agctctggcc aggcgactcc atggacgacg
     1381 ccgggtcgtc aggccctccg ccgcgtcctc cgctctgctc ccgttggaac ggtcgtccac
     1441 acccaggatc atggctgggc ggtctacgtc ggtcgcggcg cgagtggcgg cgtcggtgtc
     1501 ttcctctgcc aacgcgacaa ccgcattcgc tccctgacgg agtacaggaa gatcgacaat
     1561 ctcccggatg acgtcgttgt cagcattccg gaagcgttga tcgagccgcc ggaactcgtc
     1621 agcgatgcca ccgagctcgt cccggccgac gaactggccc ggatctggaa cgatctcaac
     1681 gaccttggac tgccggatct cgacaagctc gcgaacgaac accgcgccgc ccaggcggag
     1741 cggatcgcga gcgagagcga acgcatccag ggtgaaatcg ccgcggccca ggaggagatc
     1801 tcgcagctgc gggtcgagat gaaggagcat cccttccacg acacggagtg gcgcaagcag
     1861 taccagcgtt tccttcgccg tcgggacaat ctcgagaagg aacgcaacgc cctcaacgac
     1921 atcctcgacc gcgagatcga ggccgaggac gtgcgaatcc ggaacgtgat ccagggtatc
     1981 cgcgatgttc tccatcgctt tggctacatg cgacgcggct atccgaccga gaaggcggac
     2041 atgctcgccg atgtcttcga cacggacggt ctgatcctct gcgaactggt cgatcgcggg
     2101 atgctggagc cgctggcgcc ggaggagctc gccgagctct tctcctggta cagcttcgat
     2161 cgcgacttcc gttacgcgaa ccacttcacg ttgccggagc ggctcgtcaa cgttcgtcgc
     2221 cgcctcgagg aactcgagcg cgacgtcctc ggcgaggagc gcgatcacaa gctcttcatc
     2281 tcggaagggc acaacccgag tttctacggc gccgcactgc attggagccg tggctggtcg
     2341 atggtccaga tcggcgagca gatggagctc tccgagggcg atctcgtcct gaccttcaac
     2401 aagacgatcg acctcatgcg ccaggtccgg gacatgctga tggatgtcca gccggaacat
     2461 ccgctgatcc cgaacctcca ccgcgcggag cggctcctgc gccggggtat cgtcgaacag
     2521 tcgctgacac tcggcttcag cccgatcgcg gacgccttcg aggccgaagc cgaggatcag
     2581 ccggtcgacg gccctgccga cgatcccgat acggacgaag acgcggcgac cgccatcgtc
     2641 tgagcgcccg cggaccgcgc aaaggaggaa ccacagccat gcatcgcacc gatcggctga
     2701 ccggaatcct cctcgcgcta caagccggtc cccggagcgc ctccgaactg gccgcccgct
     2761 tcgaggtctc gaagcggacg atcatgcgcg atgtcgatgc gctcgaccag atcggcgttc
     2821 ccgtcattgc cgagactggc cgcaacggtg gctatcgcat cgccgatggc tactggatgc
     2881 caccgctgca gctgacggcg gacgaggcga cagttctcct cttcgccctc cagcatgtcg
     2941 gcagcgtcga tcgctctccg ttgggtgacg cccaccggac cgtcctcgaa aagctccagg
     3001 cgcatctcac gcccggcatc gccccggatg tcgtgaagaa tctcgacacg atggtcgtgc
     3061 tccgcgatca cgatgcgccg gatgccgcga tcatcgcgct cctccgggag gccgttgccg
     3121 cggaatgctg gatcgcgatc gtctacgcgg gcggctccgg tccctccgaa cgctcgatcc
     3181 tgccactccg cgtctatacc tcgggcggtc gctggtatgc cgatgccgtc gacag
//
LOCUS       DASTMD010000634         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2380732, whole genome shotgun sequence.
ACCESSION   DASTMD010000634 DASTMD010000000
VERSION     DASTMD010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2380732"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /gene="ppk1"
                     /locus_tag="VFQ54_09350"
     CDS             <1..1322
                     /gene="ppk1"
                     /locus_tag="VFQ54_09350"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEU0165241.1"
                     /translation="VRVDVDRAMPAEVRDWLLEQLHAGIDDLYVINGPLALQDFFELS
                     RIDRPDLKDASFTSAPMFPEPLTVPQTGEVYPDIFALIRDQDVMVHLPFQSFGTVVDF
                     LKAASTDPDVVAIKQTLYRLGKNSPLIPALIDARDDDTQVAVLVELKARFDEENNISW
                     AQTLETHGVHVAYGLAGLKTHCKLTLIVRREPDGLRRYVHISTGNYNANTARIYEDVA
                     IFTCRDDIASDVTELFNVLTGYAKQDTYRKLWVAPQGLREHFVTAIEAEIEAHKRTGA
                     GHIIMKMNSLVDRTTIRALYKASQAGVKIDLIIRGICCLRPGVTGWSETISVRSIVGR
                     FLEHSRIFYFHNGGEPKVYIGSADVMERNFDRRVEVITPVESPELAQHLRDVVLDGYL
                     RDTVNAHVLHEDGSYVPARATRAKDKFDVQAWQLELYRTGSPVGSNS"
     gene            complement(1411..1650)
                     /locus_tag="VFQ54_09355"
     CDS             complement(1411..1650)
                     /locus_tag="VFQ54_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165242.1"
                     /translation="MIETDEALEPSKDPFSLFTEDIIYDQRSIPALEQPDKAPVLEVY
                     AKGKGPGNPDIVTIRTEAYIIDWTERLHPIKSTAD"
     gene            complement(1634..1984)
                     /locus_tag="VFQ54_09360"
     CDS             complement(1634..1984)
                     /locus_tag="VFQ54_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165243.1"
                     /translation="MPPRDYVRYLDYDGYPIRIRVEFETDRRGQVIRFTVQLEIEIAE
                     MWLPVVRFDTEHNEAHIDLLDPDGREYDKQWLGIHLQYNATLTRATDELSENYGRHVR
                     RFLEQKRRTSDRDR"
     gene            2188..3006
                     /locus_tag="VFQ54_09365"
     CDS             2188..3006
                     /locus_tag="VFQ54_09365"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165244.1"
                     /translation="MIFNDPDCIAALTPLNPFERSDDGRPRVPDDLLERLKLVTNDEA
                     WGTIEKGHNYHFQFEGNWVHLHDNHVLVGRAVTAAFVPVRPDFNDVVQGIGAAEGRDG
                     GQNTWVIDTLQPGDVLVVDLFGKIEDGTFIGDNLGTATRARTGTGIIIDGGIRDFTRV
                     DELTDFPVYCRGVHPSAIKDVTLVQVNGPVRIGNATVLPGDAILGTREGVTFIPPHLV
                     QEVVEKSEDTRFRDVFGKLRLAEGKYTSGDIDRSGWADHIEADFQEWAATYSGN"
BASE COUNT          575 a          951 c          966 g          637 t
ORIGIN      
        1 cggtccgggt tgacgtcgac cgcgcgatgc cggccgaggt tcgggattgg ctgctcgagc
       61 agctccacgc ggggatcgac gacctgtacg tgatcaacgg accgctcgcg cttcaggact
      121 tcttcgaact ctcgcggatc gatcgacccg acctcaagga tgcatcgttt acctcggcgc
      181 cgatgttccc agaaccgctg acggtgccgc agaccggaga ggtctatccg gatatcttcg
      241 cgctgatccg ggatcaggat gtgatggtcc acctgccgtt ccagtccttt ggcacggtgg
      301 tcgatttcct gaaggcggcc tcgaccgacc ccgatgtcgt cgcgatcaag cagacgctct
      361 accgcctcgg caagaactcg ccgttgatcc cggcgctgat cgacgcgcgc gacgacgaca
      421 cccaggttgc cgtgctcgtg gaactcaagg cgaggttcga cgaggagaac aacatctcgt
      481 gggcgcagac gctcgagacc catggcgttc acgtcgccta cggtctcgcg gggctcaaga
      541 cgcactgcaa gctcacgctg atcgttcgcc gcgagccgga tggactacgc cggtacgtcc
      601 acatctcgac cggaaactac aatgcgaata cggcccggat ctacgaggac gtcgcgatct
      661 tcacctgccg ggacgacatc gcgagcgatg tcacggaact cttcaatgtg ctgaccggct
      721 atgcgaagca ggatacctac cgcaagctgt gggtagcgcc gcagggattg cgcgagcact
      781 tcgtgacggc gatcgaggcc gagatcgagg cgcacaagcg caccggcgcc ggtcacatca
      841 tcatgaagat gaactctctc gtcgaccgca cgacgatcag ggcgctctac aaggcgtcgc
      901 aggcgggggt gaagatcgat ctcatcatcc ggggcatctg ctgcctgcga ccgggtgtca
      961 ccggctggag cgagacgatc agcgtgcgca gcatcgtcgg tcggttcctc gaacacagcc
     1021 ggatcttcta ctttcacaac ggtggcgagc cgaaggtcta catcgggagc gccgatgtga
     1081 tggaacggaa cttcgaccgt cgggtcgagg tgattacccc ggttgaatcg cccgaacttg
     1141 cgcagcacct gcgtgacgtc gtcctcgacg gctacctccg cgatacggtg aatgcccacg
     1201 ttctccatga ggacggttcc tacgtgccgg cccgcgccac gcgggcgaag gacaagttcg
     1261 atgtccaggc ttggcagctg gaactctatc gcaccggttc gccggttggg tcgaattcct
     1321 gatccatctc cggtcttcca gggaaatgca gaacgccagc ccatgttctc gggctggcgt
     1381 tgtgggtgga atgtcctgaa agcggtggga tcagtctgcg gttgacttga tcgggtgcag
     1441 gcgctcggtc cagtcgatga tgtatgcctc ggtccggatg gtgacaatgt ccgggtttcc
     1501 cgggcctttg cccttcgcgt acacctcaag cacgggcgcc ttgtctggct gttcgagcgc
     1561 ggggatgctc cgctggtcgt agatgatgtc ttccgtgaac aacgagaacg gatccttcga
     1621 tggctcaagg gcctcatctg tctcgatcac tggttctcct cttctgttcg aggaagcgac
     1681 ggacgtgtcg cccgtagttt tcggaaagtt catcggtggc tctcgtcagg gttgcgttgt
     1741 attgcaggtg gataccgagc cactgcttat catattctct tccgtccggg tccaggaggt
     1801 caatgtgtgc ttcgttgtgt tcagtgtcga agcggactac cgggagccac atctcagcaa
     1861 tctcgatttc gagttgaacc gtgaagcgaa taacctgccc gcggcggtcc gtctcgaatt
     1921 ccacgcggat tcggatcgga tagccgtcgt agtcgaggta tcggacgtaa tcgcgaggtg
     1981 gcaccgatcc tccttctcta tttcgggtgg cggttccctt cgctgagcat acattgtccc
     2041 ccggtgaagc gtccttgccg gcgacttcac catcgtcttg cgctccccgg aacaatccgt
     2101 atagtgaccc gtacaccacc tgtagcggtg aaaaggcgtc cggtaggccg acctcttcac
     2161 gatgatgtga acccggggag atgaccgatg atcttcaacg atcccgattg tattgctgcg
     2221 ctgactccgc tcaacccctt tgagcgttcc gacgatggcc gtccccgcgt gcccgacgat
     2281 ctcctcgaac gactcaagct ggtcacgaac gacgaggcct ggggaacgat cgagaagggc
     2341 cacaactacc acttccagtt cgaaggcaac tgggttcatc tccacgacaa tcacgtcctc
     2401 gtcggtcgcg ccgtgaccgc ggcgttcgtt ccggtgcggc cggacttcaa cgacgtggtt
     2461 caggggatcg gggccgccga aggacgcgac ggcggccaga acacctgggt catcgatacc
     2521 ctgcagccgg gcgacgttct cgtcgtcgac ctcttcggca agatcgagga cggtaccttc
     2581 atcggcgaca atctcggaac ggcgacgcgt gcccgcaccg gcaccggcat catcatcgat
     2641 ggcggcatcc gcgactttac ccgggtggat gaactgaccg acttccccgt ctactgccgc
     2701 ggcgtccatc ccagtgcgat caaggacgtc acgctcgtgc aggtaaacgg gcccgtgcgt
     2761 atcggcaacg cgaccgtgtt gccgggtgat gcgatcctcg gcacacgcga gggcgtgacc
     2821 ttcatcccgc cgcatctcgt gcaggaagtc gtggagaagt cggaggacac ccgcttccgc
     2881 gatgtcttcg gcaagcttcg tctcgccgaa ggaaagtaca cgtcaggtga tatcgatcgt
     2941 tcgggttggg ccgatcacat cgaggcggat tttcaggaat gggccgctac gtattccggt
     3001 aactagagaa ttcgccgcga gtaggggttc cggggggggc ggggatgctc cctgcctcct
     3061 gggtctcatc gtgaaacgag aggtttgacg catgtccgaa ctcgaagcac cggtgccggt
     3121 gattccgcc
//
LOCUS       DASTMD010000635         3753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2827305, whole genome shotgun sequence.
ACCESSION   DASTMD010000635 DASTMD010000000
VERSION     DASTMD010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2827305"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..758
                     /locus_tag="VFQ54_09370"
     CDS             129..758
                     /locus_tag="VFQ54_09370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU0165245.1"
                     /translation="MIFEHGQKILFIGDSITDCGRRDTFAPYGNGYVSLVRGFVDARY
                     PELALRWENRGIGGNTVRHLDERWQQDVIDEKPDWLSVKIGINDVWRTFDSGGNGAVP
                     IDEYETTLRSLLQRAIDGTGCKLILAEPFDIEPNREDPMRVMMDAYGLVARKVAAEFG
                     AIDIRTQEAFDTALETTTSQTWANDKIHPGLPGHAVIALAYLRAIGFEL"
     gene            771..1352
                     /locus_tag="VFQ54_09375"
     CDS             771..1352
                     /locus_tag="VFQ54_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0165246.1"
                     /translation="MSTIKTAPTTLIPITFDAYIRYGDAIAGIYERGFHEVAGAGEQF
                     LNGQLRKHMGYAGFAGLIALDETSRAVGFVYGYRSEPGQYWREWILPDLAASGLESWL
                     GDLFEFVEFAVDPDFQGRGIGGRLHDALLLETTERRSLLCAEQGDAIAPTMYRRRGWV
                     PLIRDFKGEDLMLMGLDLAAFRARRGGTLPFVR"
     gene            1382..1930
                     /locus_tag="VFQ54_09380"
     CDS             1382..1930
                     /locus_tag="VFQ54_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0165247.1"
                     /translation="MQHVQIRPLSPSEIEAHRLDLGRIHAAAFSRDDAAGSRYAEISL
                     PEMVAYPDFLATVAIAEEQIVGFSCGHAATPHLPWSDRVYRSLRDAGHDAWTRDPFEI
                     ADVAVDPAWQNRGIGRLLLDDLIDQITQPRAILVTYHGDHPAKRFYLRAGWTVLVDDF
                     FYVPDRPPTSILGLDRHARMAN"
     gene            2036..>3753
                     /locus_tag="VFQ54_09385"
     CDS             2036..>3753
                     /locus_tag="VFQ54_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165248.1"
                     /translation="MEIATDHTGHDRSITPTNGIESLADYRPEPVAGKREVDLYVRTY
                     MTLLQSSGAISVASLEPAHLMAGAALHAGAEEPEPDLNAFLYSAQRIPACIDYIKHII
                     LGQTHRAFVRGGYPEVSEWSSQSAPGRRRRWYWDGGEQLAAYFASTSDLDDLIPSLVA
                     YEIEWNKMNRLFETQPDVRKLAIAAAKARPDATIDQEIQSALLIGDSNWARLQAVWGE
                     SFWETMALVAGGKKRIDLRMLGGTYLGYGRSVRNWWRSSFSVLSERGLHDRPVYFVSS
                     NTHSIVNTLSGVARRRQQELTDFIVETHHPELEPELRKLEAGETTGSWENLLYFAARP
                     YFHGPGKDDIRTKRQEEEHSIGISHIEPSGAVDVGVQIFELDKLNPDWFDSRLQGYDP
                     RESDAIVININYPLGMAAYHIMSQIGVSTDQLKGIYILGKAATLNGRIGDIMTSNVVF
                     DEHSGNTYWFDNAFGYDKVAPFLQFGAALDNQKAVTTRGTFLQNQGYLDFYYRENYTV
                     VEMEAGPYLSAIYEDVFLERHPTNQAINLAPLSEHLDLGIIHYASDTPYTRAHTLGAR
                     GLSFYG"
BASE COUNT          710 a         1212 c         1138 g          693 t
ORIGIN      
        1 cgcaaacctg agcgagatca tcgggacgag acgaatgacc aggtttcgac gggattgacc
       61 gcgtctcctg atagaatccc gccattggaa tggcgtcgat cgcgttgact cagttgggga
      121 gcagcctcgt gatattcgaa cacggtcaga agatcctctt catcggtgac agcatcaccg
      181 actgcgggcg tcgcgacacc ttcgcgccct atggcaacgg ctacgtgagc ctcgttcgtg
      241 gcttcgtcga tgcgcgctat ccggaactgg cgctgcgctg ggagaatcgg gggatcggcg
      301 gcaacacggt tcgtcacctc gacgagcgct ggcagcagga tgtgatcgac gagaagccgg
      361 actggctttc ggtcaagatc ggcatcaacg atgtctggcg caccttcgac agcggcggaa
      421 acggcgcggt gccgatcgac gagtacgaga cgacgctccg atccctgctc cagcgtgcga
      481 tcgacggcac gggctgcaag ctgatcctcg ccgagccatt cgatatcgaa ccgaatcgcg
      541 aggatccgat gcgggtgatg atggacgcct atggcctcgt cgctcgcaag gtcgccgccg
      601 aattcggcgc gatcgacatc cggacgcagg aggcgttcga tacggcgctc gaaacgacga
      661 catcccagac ctgggcgaat gacaagattc atccggggct ccccggtcat gcggtgatcg
      721 ccctcgccta tctccgcgcc atcggcttcg agttgtaggg gcagtcgccg gtgtcgacca
      781 tcaagaccgc gcccaccacg ctgatcccga tcacgttcga tgcctacatc cgctacgggg
      841 atgccatcgc cggcatctat gaacgcggct tccacgaggt cgcgggcgcc ggcgagcagt
      901 tcctcaacgg acagcttcga aaacacatgg gatacgccgg attcgccggg ctcattgccc
      961 tggacgagac cagtcgcgcg gtcggcttcg tctatggcta ccggagcgaa cccgggcagt
     1021 actggcgcga gtggatcctg cccgatctcg ccgcgtccgg gctcgaatcg tggctcggcg
     1081 atctcttcga gttcgtcgag ttcgcggtgg atcccgattt tcaggggcgc gggatcggag
     1141 ggcgtcttca cgacgcgctc ctcctggaga cgacggagcg gcggtcattg ctctgcgccg
     1201 agcagggcga tgcgatcgcg ccgacgatgt accggcgtcg cggctgggtt cccctcattc
     1261 gggatttcaa gggcgaggat ctgatgctga tgggtcttga cctcgccgcc ttccgcgccc
     1321 gtcgcggcgg cacccttccc ttcgtacgct gacatcgaac aaatctctgg gagacgcgtc
     1381 catgcagcat gtccagatac ggccgctctc tccctccgag atcgaggcgc accgcctcga
     1441 tctcggacgc atccatgcag ctgcattcag ccgcgacgat gccgccggga gccggtacgc
     1501 cgagatctcg ctcccggaga tggtcgctta tcccgatttt ctggctacgg tagcaattgc
     1561 agaagagcag atcgttggct tctcctgtgg acacgcggca acgcctcatc tgccatggtc
     1621 ggatcgggtc tatcgcagcc tccgcgacgc cggacacgac gcgtggacgc gcgatccgtt
     1681 cgagatcgcc gatgttgccg tcgacccggc ctggcagaac cggggcatcg ggcggctcct
     1741 tcttgacgac ctcattgacc agatcaccca accccgagca atcctcgtga cctaccacgg
     1801 cgatcatccg gcgaagaggt tctatcttcg cgctggttgg accgtgctcg tcgacgactt
     1861 tttctatgtc ccggaccgcc caccaaccag cattctgggt ctggatcggc atgctcgaat
     1921 ggcgaattga ccctcccgta acacacgttt gcatcagttg tgctacacca gtgggcaaca
     1981 aacgagatgg aaacgtgaga gcggtcagga tggccgcatt cgaggagcag gtcacatgga
     2041 aatagctacc gatcacactg gtcacgatcg atcgattacc ccgacgaacg gcatcgagag
     2101 cctcgccgac tatcgcccgg aaccggtcgc cgggaagcgc gaagtcgatc tttacgttcg
     2161 aacgtatatg acgctcctgc agagttcggg cgcgatcagc gttgcctcac tcgagccggc
     2221 gcatctcatg gcgggcgcgg ccctccatgc cggtgccgaa gaacccgaac ccgacctcaa
     2281 cgccttcctc tattcggccc agcgcatccc cgcctgtatc gactatatca agcacatcat
     2341 ccttgggcag acgcaccgcg cgttcgtcag gggaggctat ccggaggtct cggagtggtc
     2401 ctcgcagtcg gcaccgggac gtcgccgccg ctggtactgg gatggtggcg agcaactcgc
     2461 cgcgtatttc gcctcgacat cggacctcga cgatctgatc ccctcgctcg ttgcctacga
     2521 gatcgagtgg aacaagatga atcgtctctt cgagacgcag cccgacgtgc gcaagctcgc
     2581 gatcgccgcc gcgaaggcgc ggccggatgc cacgatcgat caggagatcc agtcggcgct
     2641 cctgatcggg gacagtaact gggctcgcct gcaggcggtc tggggcgaat ccttctggga
     2701 gacgatggcg cttgtcgccg gtggcaagaa gcggatcgat ctccggatgc tcggcgggac
     2761 ctatctcggc tacgggcgct ccgtccgcaa ctggtggcgg tcgtcctttt cggtcctcag
     2821 cgagcgcgga ctgcacgatc gaccggtcta cttcgtctcc tcgaatactc acagcatcgt
     2881 gaacacgctc tccggcgtgg cacggcgtcg ccagcaggaa ctcacggact tcatcgtcga
     2941 gacgcatcac ccggagctgg agccagaact gcgcaagctc gaggcgggcg agacgaccgg
     3001 gtcgtgggag aacctgctct acttcgcggc gcgtccctat ttccacgggc ccggcaagga
     3061 cgacattcgg acgaagcggc aggaggaaga acactcgatc gggatctccc acatcgagcc
     3121 gtcgggcgcg gtcgatgtcg gcgtccagat cttcgaactc gacaagctga atccggactg
     3181 gttcgattcc cgcctccagg gctacgatcc gcgggaatcc gatgcgatcg tgatcaacat
     3241 caactacccg ctcgggatgg cggcgtacca catcatgtcg cagatcggtg tctcgaccga
     3301 ccagctgaag ggaatctaca tcctcggcaa ggcggcgaca ctgaacggcc gcatcggcga
     3361 catcatgacc tcgaatgtcg tcttcgacga gcattccgga aatacgtact ggttcgacaa
     3421 cgccttcggc tatgacaagg tcgccccatt cctgcagttc ggcgccgctc tcgacaacca
     3481 gaaggcggtg acgacgcgcg ggaccttcct ccagaaccag ggctatctcg atttctacta
     3541 tcgggagaac tacacggtcg tcgagatgga agcgggaccg tatctcagtg cgatctacga
     3601 ggacgtcttc ctcgaacgcc acccgacgaa ccaggcgatc aacctcgcgc cgctcagcga
     3661 gcacctcgat ctcggcatca tccactacgc gtcggacacg ccatacacac gcgcccacac
     3721 gctcggtgcg cgcggcctct ccttctatgg cat
//
LOCUS       DASTMD010000636         1870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2559327, whole genome shotgun sequence.
ACCESSION   DASTMD010000636 DASTMD010000000
VERSION     DASTMD010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2559327"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1342
                     /locus_tag="VFQ54_09390"
     CDS             17..1342
                     /locus_tag="VFQ54_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165249.1"
                     /translation="MSAAIAIAGIVVGVIIMCILLFRGVHPFVAAIIGSVIIAITGQD
                     GLYDALKNQYMGGFADFFGSNFLIFVAGALFGKVFEITNAAKSIANMLVKACGKRFAL
                     IGLSLATGIMSYAGIVGFVLCFAVWPIAVEICRESDIPRRFAPAAIVMGCVTYSGFGP
                     FNAQVANVAVASSLGTDLGAAWKVSLIGVAFSVVITAVILMTLIARARSKGEHFVALE
                     SDGANASGTENLPNGLLALIPIVVTVLLINIKVGGSAILQPEFGVFVGAVLAYALMFR
                     HVAKDGVSLWKRAAEAVVNAIGAAAGTSAMVAIGSVVKVSAGWDTMIGAIVNIPGPPL
                     VAVAVGGMVMGAIMGSGTGAAGLTAPMFQPIFGPLGVSPVALHRTIVASTLAGGTLPN
                     CGFFNTMILGIAKSNYKECYGPIFWTCPFSNFLSTIVMVVLMSVFPSLV"
     gene            1379..>1870
                     /locus_tag="VFQ54_09395"
     CDS             1379..>1870
                     /locus_tag="VFQ54_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEU0165250.1"
                     /translation="MNQPSGHAESGPAVVSDLLRRNATRNPDRAALVEVDSHVVRGVS
                     GPDGAASGRSLTWREFDEAADKVAGYLIDRGIGPGSAVGIMMRNRLEWIPIYFGILRS
                     GAVAVPLNFRDKSDDICGKAERVGIRALVFGCYASCEVAVARWTLSPQTDFVFVGEEK
                     SRPG"
BASE COUNT          293 a          556 c          625 g          396 t
ORIGIN      
        1 aaggactatg ctccaaatga gtgcagcaat agcaattgcc ggcattgttg tcggtgtcat
       61 catcatgtgc atcctcctgt ttcgaggcgt gcacccgttc gtcgccgcaa tcattggctc
      121 cgtgatcatt gcgatcacgg gtcaggatgg cttgtatgac gcgctcaaga atcagtacat
      181 gggtgggttc gctgatttct tcgggtccaa tttcttgatc ttcgtggccg gtgccctttt
      241 cggcaaggtc tttgaaatca ccaatgcggc gaaatcaatt gccaatatgc tcgtcaaggc
      301 ctgcggaaag agattcgccc tcatcggcct ctctctggcc acgggaatca tgagctatgc
      361 ggggatcgtg ggattcgttc tctgcttcgc ggtctggccg atcgcggtcg agatctgccg
      421 tgagtcggac attccccgca ggttcgcacc cgccgccatc gtgatgggat gcgtgacgta
      481 ctccgggttc gggcccttca acgcccaggt cgcgaatgtg gcggtcgcca gctcgttggg
      541 cacggacctc ggtgcggcct ggaaggtgtc gctcatcggg gtcgcgttct cggtcgtcat
      601 cactgccgtc atcctgatga cgttgatcgc tcgggcgcgc tccaagggag agcacttcgt
      661 cgccctcgaa tcggacggtg cgaacgcgag cgggacggag aacctcccca atggcctcct
      721 cgcgctcatc ccgatcgtgg tcacggtcct gctcatcaac atcaaggtgg gcggctcggc
      781 catccttcaa ccggagttcg gtgttttcgt cggagccgtc ctggcctacg ccctgatgtt
      841 ccggcacgtc gccaaggacg gagtctcact gtggaagcga gcggcagagg ctgtcgtcaa
      901 cgcgatcggt gcggccgcgg ggacgtcggc gatggtcgcc atcggaagcg tcgtcaaggt
      961 ctcggccgga tgggacacga tgatcggcgc gatcgtcaac atccccggcc cgccgttggt
     1021 cgcggtggcg gtgggcggca tggtgatggg cgcgatcatg ggttcgggaa ccggagccgc
     1081 agggctgacg gcgccgatgt tccagccgat ctttggcccc ctgggagtct ctccggttgc
     1141 cctgcaccgc acgatcgtgg cgtcgacgct ggcgggcgga acactgccca actgtggatt
     1201 cttcaacacg atgatcctcg ggatcgccaa gtcgaactac aaggagtgct acgggcccat
     1261 cttctggacc tgccccttct cgaacttctt gtcgacgatc gtcatggtcg ttctcatgag
     1321 cgtgttcccg tcgctcgtct gatcccgtgt gtggatgcct gagaggggag cgcggactgt
     1381 gaaccagccg agtgggcacg ccgagagtgg acccgcggtg gtctccgacc tgttgcgtcg
     1441 caatgcgacc cggaatccgg accgggcggc cctggtcgag gtcgattcgc acgtcgttcg
     1501 cggtgtcagt ggtcctgacg gtgcggcgtc cgggaggtcg ctgacctggc gggaattcga
     1561 cgaggccgcc gacaaggtcg ccgggtactt gatcgatcgc gggatcgggc ccggctccgc
     1621 cgtcggaatc atgatgcgga accgcctcga atggattccg atctatttcg ggatcctccg
     1681 gtcgggagca gtcgccgtgc cgttgaactt tcgcgacaag tcggacgaca tctgtggcaa
     1741 ggcggagcgg gtggggatcc gggccctcgt gttcgggtgc tacgcctcct gcgaggtggc
     1801 ggtggcgcgg tggacgctct ccccgcagac cgacttcgtc ttcgtcggag aggagaagag
     1861 ccgtccggga
//
LOCUS       DASTMD010000637         4832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1755576, whole genome shotgun sequence.
ACCESSION   DASTMD010000637 DASTMD010000000
VERSION     DASTMD010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755576"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFQ54_09400"
     CDS             complement(<1..389)
                     /locus_tag="VFQ54_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0165251.1"
                     /translation="MTDRLYMLDTDTASYWIKGVHRSVLETISSIPRDQICISVVTRA
                     EILIGLKNMASTRPDYIVSRRFLRDVVTKAWDNDVADVYADIHDDLRRRGELFGERDV
                     MIAAHAIALGATLVTNNTRHFARIAPPL"
     gene            complement(386..709)
                     /locus_tag="VFQ54_09405"
     CDS             complement(386..709)
                     /locus_tag="VFQ54_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEU0165252.1"
                     /translation="MAEVKIAKLFQNGGSQAVRLPAEFRFEGDEVFISRDATTGAVIL
                     SDHAQPSTWADFIAFRDSFKISDEERDEFDRVMNDIIDERARDTGAERDAYLDKLFGE
                     NEDRE"
     gene            complement(927..2447)
                     /locus_tag="VFQ54_09410"
     CDS             complement(927..2447)
                     /locus_tag="VFQ54_09410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165253.1"
                     /translation="MSTATASISSPMLAPALDPRRWIALAIVSIAQLMIIVDSAIVLI
                     ALPPMQKALHISTTDRQWVVTAYTLAFGGLLLLGGRIADYLGRKRVFIIGLLGFAVAS
                     ATGGLATEAWMLFASRAVQGAFAAIMAPAALSLLTVTFTDPRERAKAFGVFASVAGGG
                     ATIGLVAGGLLTEYASWRWCLLVNVPIGLLTALAGYQVLRESKAEGSAHYDIPGAVTV
                     TAGLVSLVYGSSRAADHGWSSAGTLSFLVAAAVLMVAFVVIEWRVANPLLPLRVITER
                     NRAGAFLASLLVGAGIFGMSVFLSYYLQVSLGYSAVKTGLAFVPFSIGTIVGAMVASK
                     ILPLVPPRIGLIVGLVPTTVGMIMLATVDVDSGYVTRILPALIVTSIGLGLAFVSFTS
                     ASLFGVAPNDAGVASALVNATQQIGSTIGAAVLNTVAITATTSYLKDHGGASSFLAAA
                     TVHGYDRVFLIAGVVIAVSVLLVLVLVNAKAEGAPTDSGIGEVDRRGGVSQALGTD"
     gene            2586..3191
                     /locus_tag="VFQ54_09415"
     CDS             2586..3191
                     /locus_tag="VFQ54_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0165254.1"
                     /translation="MPRISREEKERNHQKILEAASRRFRSEGLDAVGIDELMKTAGMT
                     HGGFYNHFPSKDALAAAVCQQAFAASHVAIADEVATGAPLCEILRLYLSPEHRDDPDG
                     GCPSAALVIDAGRHVGAIQDAYRDGVLGYLTDFTDAIVQDAANRGETLDVATARQRAM
                     LLLSQVVGAMAISRAIVSSDRPLSDEILATVRDNLQPDSNP"
     gene            complement(3466..4245)
                     /locus_tag="VFQ54_09420"
     CDS             complement(3466..4245)
                     /locus_tag="VFQ54_09420"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0165255.1"
                     /translation="MTELKAVLLDLDDTIIYDDATTEIVFASVARHAHEVAGVDPAQL
                     IAGVDRHAHAIWADGPAPEWCHDLGTSATEGLRSLFPGDDPRMAAMRAWGPGFRFGSW
                     RRALADSGIEDDDLARELDRRFAEERLGTNDFIPGADVALRELAGRFRLAMITNGLSD
                     VQRVKVEGTGIGSLFEVIVVSGELGFGKPNPEIYHHTLRTLGVAPDEAIMVGDNIRRD
                     VAGSQAVGMRGVWIAESAPSAESSIHPDITIRTLAELPALI"
     gene            complement(4354..4767)
                     /locus_tag="VFQ54_09425"
     CDS             complement(4354..4767)
                     /locus_tag="VFQ54_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU0165256.1"
                     /translation="MRVLLDTNVVSEFAKGPSPVDALVDSIPDADIFISTLSMGEIQK
                     GISLRPVGRGRLELENWFEELKGLYADRSILFDLEAALIWGELAASTICAGRNIGTVD
                     LQIAAIAFQHDLTVITRNVRDFIPTGVPVINPWDS"
BASE COUNT          976 a         1538 c         1471 g          847 t
ORIGIN      
        1 agcggtggcg cgatgcgcgc gaagtgacgc gtgttgttcg tcaccagcgt cgccccaagc
       61 gcgatcgcgt gagcggcgat catcacatcg cgctccccga aaagttctcc tcgacgcctg
      121 aggtcatcgt gtatatcggc atagacatcg gcgacgtcgt tgtcccatgc cttggtcacg
      181 acatcccgaa gaaatcggcg actcacgatg taatcagggc gcgtggaagc catattcttg
      241 aggccgatca ggatctcggc cctggtgaca acggatatgc agatctgatc gcgcgggatt
      301 gatgagatcg tctccagaac ggagcggtgg actcccttga tccagtagct tgccgtatcc
      361 gtgtcgagca tgtagagacg gtccgtcact cacgatcctc gttctcgcca aagagcttgt
      421 cgagatacgc gtcacgttcg gctcccgtat cccgtgcgcg ctcgtcgatg atgtcattca
      481 tgacccgatc gaactcatcg cgttcttcgt cggaaatctt gaacgagtca cgaaaggcaa
      541 tgaaatcggc ccaggtggaa ggttgggcgt ggtcggagag gatcaccgcc cctgtcgtcg
      601 cgtcccggga gatgaaaacc tcgtcgcctt cgaatcggaa ctcggcgggc agacgcacgg
      661 cctgactccc gccgttctga aaaagcttcg caatcttgac ctcggccatc gcgcacctcc
      721 tgatctggtg tatatacgtg atatataccc ctccccctcg cgctcgtcaa gttatgtcag
      781 atcgactcag gcaggtcggc gaggggcagc tcgcgacgtc cggctggttc gctgacctcc
      841 gcccttcacc acgcgacgca caaggggatg gcacgccagg cgatcgccca gcatgccatc
      901 ctcgaacaga tttccttgcg cgaggcctag tcggtcccga gggcctggct tacgccaccg
      961 cgacggtcga cttcgccaat cccggaatcg gttggcgcac cctctgcctt cgcattgacg
     1021 aggaccaaca cgaggagcac gctcaccgca atcacgacac cggcgatcag gaagaccctg
     1081 tcgtagccat ggacggtcgc ggccgccagg aacgacgatg cgccaccgtg gtccttgagg
     1141 tagctcgtcg tcgccgtgat cgccaccgta ttcagaacgg ccgccccgat cgtgctgccg
     1201 atctgttgcg tggcattgac caatgcgctg gcgacgccgg cgtcgttcgg cgcgactccg
     1261 aacaggctcg cgctggtgaa cgagacgaat gccagaccga gtccgatgct ggtgacgatg
     1321 agcgcgggga ggatgcgggt gacatagcca ctatccacgt ccaccgtggc cagcatgatc
     1381 atgccgaccg ttgtgggcac cagtccgacg atcagtccga tccgaggcgg tacgagcggc
     1441 aggatcttgc tggccaccat cgcgccaacg atcgtcccga tcgagaacgg cacgaatgcg
     1501 agcccggtct ttaccgccga gtagccgagg ctgacctgaa gataatacga gaggaaaacg
     1561 gacatcccga agattccagc gccgacgagc agcgatgcga ggaatgctcc cgcccggttg
     1621 cgctcggtga tgacgcgcaa cgggaggagg ggattggcga cgcgccattc gatgaccacg
     1681 aatgccacca tcaggaccgc cgccgcgacg aggaaggaga gggtgcccgc cgatgaccag
     1741 ccgtgatcgg cggcgcgact cgatccgtag acgagcgaaa cgagacctgc ggtcaccgtt
     1801 accgcgcccg ggatgtcgta gtgggcggag ccttcagcct tgctctcgcg gagaacctgg
     1861 tacccggcga gcgcggtaag aagtccgatc ggaacgttca cgaggaggca ccatcgccac
     1921 gatgcgtact ccgtcagcag accgccggca acgagaccga ttgtcgcgcc accaccggcg
     1981 accgatgcga agacgccgaa cgccttcgcc cgctccctgg gatcggtgaa cgtcacggtc
     2041 aggagcgaca acgcggctgg cgccatgatt gccgcgaatg cgccctggac cgcacgcgat
     2101 gcgaagagca tccacgcttc cgtcgcgaga ccacccgttg ccgaagcgac ggcgaatccc
     2161 agcaacccga tgatgaagac gcgcttgcgc cccagataat ccgcgattct gccaccgagg
     2221 agcaggagac cgccgaacgc aagcgtatag gccgtgacca cccattggcg atccgtcgtg
     2281 gagatgtgca gggctttctg catcggtggc agcgcgataa gcacgatcgc gctgtcgacg
     2341 atgatcatga gttgcgcgat ggagacgatt gccagcgcaa tccatcgccg gggatcgagg
     2401 gccggggcca acatgggact ggaaatcgac gcagtagcag tactcacgat ctgacgctcc
     2461 tcatgaccgg tgcgaaggtg tatcgccttc gttgggcaaa cattaatgat gacgtttgac
     2521 atcattaatc aaggccacta caatgacgcc taacatcaaa gatgtcaagc aggaggatcc
     2581 acgtcgtgcc acgtatctcc cgggaggaga aagagcgaaa tcaccagaag attctggagg
     2641 ccgccagccg ccggtttcgg tccgaggggc tcgacgccgt cggcatcgat gagctcatga
     2701 aaaccgcggg gatgacgcac ggggggttct ataaccactt cccgtccaag gacgcacttg
     2761 ccgcggcggt ctgccaacag gccttcgccg cgtcgcacgt cgccattgcc gatgaggtcg
     2821 cgacgggcgc gccactctgc gagattctgc gcctctatct ctcaccggaa caccgcgacg
     2881 accccgacgg tggctgcccc tccgccgccc tggtgatcga tgccggtcgc cacgtcggag
     2941 cgatccagga cgcctatcgt gacggcgttc tcgggtatct caccgacttc accgacgcga
     3001 tcgtgcagga tgcggcgaac cgaggcgaga cactcgatgt agcgacagcg cgccaacgcg
     3061 cgatgctcct cttgagccag gtcgtcggcg cgatggcgat ctcgcgggcg atcgtctcct
     3121 cggatcgtcc gctttccgac gagatcctcg ccaccgtccg cgacaacctg cagccagact
     3181 cgaatccata gcactcaacg gcgtaggcgc gatgattccc acgcggcgac cgggaggagc
     3241 ggcagggcat aaaaccccgc cctaccaccc ccagcaccat cagcacgtgt cgacggttcc
     3301 ggatttcgcc gtcacacacg caggtggtcg atgtacatga cgcgtttaca gcgtagttcg
     3361 gatcgatcgc catgttgcgg acaatccggg gtctgtaggg caaggccttg tgccttgccg
     3421 cagacgggat cgtatgcacg gcgcgcgacc gccctgaact gcgctttaga tcagcgcagg
     3481 cagctcggcg agagtgcgga tcgtgatgtc cggatgaatc gacgactcgg ccgacggcgc
     3541 ggactccgcg atccagacgc cgcgcatacc gactgcctgc gatcccgcga cgtcacgccg
     3601 gatgttgtct cccaccatga tcgcctcgtc cggggcaacc ccgagtgtcc gcaaggtgtg
     3661 gtgatagatc tccggattcg gctttccgaa accgagttcg ccggagacga cgatcacctc
     3721 gaacaacgat ccgatcccgg tgccctcgac cttgacgcgc tgcacatcgg agaggccatt
     3781 cgtgatcatc gccagccgga agcggcccgc gagctcgcgc aatgcgacgt ccgcaccggg
     3841 gatgaagtcg ttcgttccga gccgttcctc ggcgaagcgc ctgtcgagct cccgtgcgag
     3901 atcgtcatcc tcgatgccgg agtccgccag tgcccgtcgc cagcttccga aacgaaaccc
     3961 ggggccccag gcgcgcatcg ccgccatccg cggatcgtcg ccggggaaga gcgaacgcaa
     4021 tccttcggtc gcgctggtgc caagatcgtg gcaccactcc ggcgccggtc catccgccca
     4081 gatcgcatgc gcgtgccgat cgaccccggc gatcagctgc gccggatcga ccccggcgac
     4141 ctcatgcgcg tggcgcgcca ccgaggcaaa gacgatctcc gtcgtcgcgt cgtcgtagat
     4201 gatcgtgtcg tcgaggtcga ggagcacggc cttgagttcg gtcacaagat atcccttcgc
     4261 ggtcgcaaag ctgatgctgg cagcacaata cgaagggaag atagcgatct ttctgtgctc
     4321 agtcgatggg atcaacaaac agactgtccg ggatcaggaa tcccagggat tgattaccgg
     4381 caccccggtg gggatgaagt cccgcacgtt gcgggttatc accgtcaaat catgctgaaa
     4441 tgcgatcgcc gcgatctgga gatccaccgt cccaatgttg cggcccgcgc atatcgttga
     4501 agccgcaagt tcgccccaga tgagagcagc ttcaagatcg aagaggattg aacgatccgc
     4561 gtaaaggccc tttagctctt caaaccaatt ctcgagttcc agccgacctc gcccgactgg
     4621 acgaagcgag atacccttct ggatttcgcc catggagagc gtgctgatga aaatatcggc
     4681 gtcaggtatc gaatcaacga gggcatcgac aggtgagggt cccttggcaa attcactcac
     4741 cacgtttgtg tcaagcagca ccctcatccg aggtcgatat cccgcatggg tcgtttgtcg
     4801 cgctcgagat cgagttctgc cagttccgga gg
//
LOCUS       DASTMD010000638         4403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2022926, whole genome shotgun sequence.
ACCESSION   DASTMD010000638 DASTMD010000000
VERSION     DASTMD010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022926"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VFQ54_09430"
     CDS             complement(<1..1031)
                     /locus_tag="VFQ54_09430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU0165257.1"
                     /translation="MSDPHSDQTLDDKVIFASQPLTEFVTRILGILMIASTTLFAAYS
                     LVELFQPGDVLAFLLLRSIGTIVLVACTAVWLGARNGEYRIAAAIPGLVFISTISICT
                     TIDTWLSHESFEIALAYGLVICVLSVLLFRIVPSLVAGFVGTLGPPLILMFSQRPDAD
                     DLVNFSGLTVITVLLAISVFIVQRKVVAELFLLNHELVAEAGRDDLTGLMNRRHWSER
                     VTGWEAEHPGAPSCIIYMDLDRFKAINDRRGHDVGDQALIAYARILKQALPAEAMAAR
                     FGGDEFVVFVPGDRDDAETFVDTLQETVQNSVLREFDLRVSIGLAQAEPGIARDELLR
                     QADQAMLAA"
     gene            1404..3869
                     /locus_tag="VFQ54_09435"
     CDS             1404..3869
                     /locus_tag="VFQ54_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU0165258.1"
                     /translation="MAHESVDDNAQLRPLAFPFFFGHGRDPHDRPPLPRTLLIGRERD
                     VAVILDLLREDDTALVTLTGPGGVGKTRLALAVSAATAGDFAHGMAIVELAAIDQPSL
                     VLPKIATTLGLSDAGQQAPIERLIAFLQLRDFLLVIENLERVPDVASEVSALLARCPR
                     LKILATSRVPLRITAEHVVAIGPLALPDAGARSMDEIAASPAVQLFVVRARATGSEFI
                     LTDENAVAVAAICTRLDGLPLAIELAAARISTLSPSEILTRLGPTLPLLTRGPRDQPG
                     RLQTMRGAIAWSYDLLEPEEQVLFRRLSVFVGGVDLDATEALVREMGTAKSGTTTGKP
                     LSSSILDGVTTLVEKSLLRRVPDTSGDGSRFQMLQTIHQFGMDLLAADPEEDTVRAAH
                     ALALLPAVERAFTKTCGPAFSTALARFEADEGNIRAALAWAVAHGETEIGLRLARATT
                     VFWVARGHSEEGAQWFAHTLAAGQTAQPLTRASTLHAAGWLARSRSDFDAAICFLDEA
                     IAISTSFAFPLIAGASQLELGMIDLLRTNVSQAMAWFQEAQRLLHDVPSSSSDLPGPT
                     GPHFVSIASYYLGQAALAEGDLPGATAHLEQAIVLQRALGFTWLLTGTLRTFGNLAHL
                     LGDRDRELAAYRESLELVKQHGDLRYLAEALCGVASVAAEQHRATEAARLFGAVASLR
                     RELGIDIGIEPWQKGRLDEGLALIRSILPPETLERAWSVGAALTREEAIAEALALVPV
                     TVTGSPLPEAVVTRTTGSVDLTPRERDVLRLVSQGLGNREIAAELFISPRTVNFHLTN
                     LFTKLSVDSRTAAAAYAIRHCLV"
     gene            4224..>4403
                     /locus_tag="VFQ54_09440"
     CDS             4224..>4403
                     /locus_tag="VFQ54_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165259.1"
                     /translation="MENIVVVTFDSPSKAYQGLSILKDLDQSLRISLQSAVVVERQAS
                     GQPIVRDGVNDYGWSG"
BASE COUNT          829 a         1437 c         1313 g          824 t
ORIGIN      
        1 ttcgcggcaa gcatcgcctg atccgcttgc cgcagcagtt cgtcgcgcgc gatgccgggt
       61 tccgcctggg cgagaccgat gctcacccgg agatcgaatt cacgcagcac gctgttctgg
      121 acggtctcct gcagcgtatc gacgaacgtc tccgcatcgt cgcggtcacc cgggacgaag
      181 acgacgaact cgtcgccccc gaagcgcgcc gccatcgcct ccgccggcaa cgcctgtttc
      241 aggatccgcg cgtacgcgat cagcgcctga tcgccgacat cgtgcccgcg ccgatcgttg
      301 atcgccttga accggtcgag atccatgtag atgatgcaac tcggcgcgcc cgggtgttcc
      361 gcttcccacc ccgtcacgcg ttccgaccag tgccgccggt tcatcagccc ggtgagatcg
      421 tcgcgaccag cctcggcgac aagttcatga ttcaggagga agagttccgc cacgaccttc
      481 cgctgcacga tgaagacact gatcgccagc agcaccgtga tcaccgtcaa cccgctgaaa
      541 ttcacgagat catcggcgtc cggtcgctgc gagaacatca ggatcagcgg aggcccgagc
      601 gtccccacga atcccgccac cagggatggc acgatccgga agagcagaac gctcagaacg
      661 cagatcacga ggccgtaggc gagcgcgatc tcgaacgatt cgtgcgagag ccaggtgtca
      721 atcgtggtgc agatcgagat cgtggagatg aagacgagtc cgggaatcgc cgccgcgatc
      781 cggtattccc cgttccgggc gccgagccag actgccgtgc aggcgacaag cacgatcgtc
      841 ccgatcgaac ggagcaagag gaatgcgagg acatcaccgg gttgaaagag ctcgacgagg
      901 gagtacgcgg caaagagcgt cgtactcgcg atcatcagga ttccgaggat ccgggtgacg
      961 aattccgtca ggggttggga cgcaaagatg accttgtcgt cgagcgtttg atccgaatgt
     1021 gggtccgaca tcacgagggc aattccagca cgaacgaatg ttacgaagaa gcccgcccca
     1081 tcgcatctat ctttccctat ctgaatctca cagtcaatcc cggcctatta tccaggccgc
     1141 tatttcgcga ataatcgccg gagacgtccg aacaggaagg ttcggctcga catcgcgagg
     1201 aggctcttca caaacacggt gcagtcaccg gattcgcgga tcgctcgtga tacgtcacaa
     1261 tggacaagct atcccggctc tgctgaaatg atgtcatggt ttggtgcctg cctcctcgta
     1321 gtcgcgactc caggtgtcgc cagatacgat cgcagcgcgc ggaccatcca actgccagaa
     1381 ttgcgaagag aggaacgagg gatatggcgc acgaatcagt cgatgacaac gcgcagcttc
     1441 gtcccctcgc gtttccattc ttcttcggtc acggtcgcga tccccacgat aggccgccgc
     1501 ttccacgcac tttgctgatt ggtcgcgaac gggacgtggc ggtgattctc gatctgctgc
     1561 gggaggacga cacggcgtta gtgacgctca ccgggcctgg gggcgtggga aagacccgtc
     1621 tcgccctggc tgtctccgcg gccaccgccg gtgactttgc gcatggcatg gcgatcgtcg
     1681 aattggcggc catcgaccag ccctccctcg tccttccgaa gatcgcaacg acacttggac
     1741 tgagcgatgc gggacagcaa gctcccatcg agcggctgat cgcctttctc cagttgcggg
     1801 acttcctcct cgtcatcgag aatctcgaac gggtccccga tgttgcatcc gaggtcagcg
     1861 cactcctcgc tcgttgtcct cgtctgaaaa ttctcgcgac gagtcgggtt cctcttcgaa
     1921 taacagcgga gcatgtcgtc gcaatcggac cactcgcgct tccagacgcc ggcgcccgaa
     1981 gtatggatga gatcgcggcc tcgcccgcgg tccagctctt cgtcgttcgg gcacgcgcga
     2041 ccggatcgga gttcatcctc accgacgaaa acgcggtggc ggttgcggca atctgtacgc
     2101 ggcttgatgg actcccgctc gcgatcgaac tggcggcggc ccgaatttcc acactctcac
     2161 cctccgagat cctcaccagg cttggcccta cgctgccact cctgacgcgt gggccgcgcg
     2221 accagccggg ccgtttgcaa acgatgcgcg gcgcgatcgc ctggagctat gacctgcttg
     2281 agccggaaga gcaggtcctc ttccgtcgtc tgtcggtctt tgtcgggggg gtcgatctcg
     2341 atgcgaccga ggcgctcgtc cgcgagatgg gcaccgcaaa atccgggacg acaaccggca
     2401 aacctctctc gtcctccatc ctcgatggcg tcaccaccct ggtcgaaaag agcctgttgc
     2461 gtcgggtccc ggatacaagc ggggatggat cccggtttca gatgctgcaa acaatccacc
     2521 aattcgggat ggacctcctc gccgccgatc ctgaggagga caccgtccgg gcggcgcatg
     2581 cgttggcgct cctgccagcc gtcgaacggg catttacaaa gacgtgtggc ccggcctttt
     2641 cgaccgccct cgctcgtttc gaggccgacg aaggaaacat ccgcgccgcc ctcgcctggg
     2701 ccgttgccca tggagagacg gagattggtc tccggttggc gcgtgcaacg acggtcttct
     2761 gggtcgcacg agggcacagc gaagaagggg cccaatggtt cgcccacacg ctcgccgctg
     2821 gtcagaccgc gcaacccctg acccgggcgt ccacgcttca tgccgctgga tggctggcgc
     2881 ggtcccgaag tgatttcgat gctgcgatct gtttccttga tgaagcgatc gcgatctcga
     2941 cctcgttcgc tttcccgctg atcgccggtg cctcacagct cgaactgggt atgatcgatc
     3001 tcctgcggac gaacgtcagc caggcaatgg catggttcca ggaggcgcag cgcctcctgc
     3061 atgatgtccc ctcgtcctca tcggacctgc cgggtccaac cgggccgcat ttcgtcagca
     3121 tcgcctccta ctatctcggc caggcggcat tggccgaggg cgatctcccc ggtgcaactg
     3181 cccatctcga gcaggcgatc gtcctccaac gcgcactcgg ctttacatgg ctcctgacag
     3241 ggacccttcg gactttcggc aatctcgccc atctccttgg cgatcgcgac cgcgaactcg
     3301 ccgcctatcg ggagagcctc gaacttgtca agcagcatgg tgatcttcgc tatctggccg
     3361 aagcgctctg cggggttgcc tcggtcgcgg cagaacaaca tcgtgctacg gaagcagcgc
     3421 gtctcttcgg agccgtggcg tcgctacgcc gggagctcgg aatcgatatc ggcatcgaac
     3481 cgtggcagaa aggccgtctg gacgaaggat tggcgctgat tcggagcatc ctgccgcccg
     3541 aaacactcga acgtgcatgg tcagtggggg cggcactgac cagggaagag gcgatcgcgg
     3601 aagcgctggc cctggtcccc gtaaccgtaa ccgggtcccc gctacccgag gctgtcgtca
     3661 cccgaacaac gggctccgtc gacctgacgc cccgcgagcg cgatgtcctg cggctcgtct
     3721 cgcaaggact cggtaaccgg gagatcgcgg cggagctctt catcagcccg cgaacggtca
     3781 atttccacct caccaacctc ttcacgaagc tatcggtgga ttcgcgcacc gccgctgccg
     3841 cctatgcgat ccgccactgc ctcgtctgat cgggcacccg acgctgatat ccctcgcaat
     3901 gcccgtcaga tggacaggtt cctgggttcc cgcgagcgga tcccccggtt atccctgtcc
     3961 aggttgccag ccccatgatt gaaacccggt cgcaaatccc cgtcaatcac acggtgtgta
     4021 gcgcaacgca cgagaactag tgttgccccg aggagaatgt gcccgcccga tttggccgtg
     4081 gcagattcct cgggattcgg catgaggtga aaccgggggc gtggatgccc ggattcacct
     4141 cgcagtgacg aggaggtaca cgccgctcgc ctaccacgat cgggaagctt caccgtcaat
     4201 atcgctccca gggagaacca atcatggaaa acattgtcgt cgttacgttc gattctccga
     4261 gtaaggcata ccagggtctg agtatcctga aagacctcga tcagagcctg cgcatttccc
     4321 tccagtccgc cgtcgtggtc gagcgacagg caagcggtca acccatcgtt cgcgacggcg
     4381 tgaatgacta cggctggtcc ggg
//
LOCUS       DASTMD010000639         1671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_416089, whole genome shotgun sequence.
ACCESSION   DASTMD010000639 DASTMD010000000
VERSION     DASTMD010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416089"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            452..>1671
                     /gene="fabF"
                     /locus_tag="VFQ54_09445"
     CDS             452..>1671
                     /gene="fabF"
                     /locus_tag="VFQ54_09445"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU0165260.1"
                     /translation="MSDIDRPRRRVVVTGTGAISAVGNTASELWDSVRNGRSGIGPIT
                     RFDPVEFETRFAGEVKDFDPASTIGRKEYRRLDRYAQFAIVATREAVTQSGIDVAADP
                     TRVAVLIGTGMGGMETFESGTETMLTQGAKRVSPFFVPMMLPNMGSGNVAIDIGARGP
                     NFATVSACASSGHAIGEGVRMIRDGRADIVVAGGAEAPVTRIGIGGFNAMGALSTRND
                     DPLAASRPFDANRDGFVLAEGAATVILEDRDYAIARGATILGEILGYATTDDANHMVQ
                     PGPGGEGIARAMVLALKDAGIVAEDLDYVNAHGTSTQLNEKFETQGYKRALGDHAYRV
                     PISSTKSMTGHLLGAAGAVEAVITMNALNDGLLPPTINQTDDDPECDLDYIPNTARKA
                     DARIALSNSMGFGG"
BASE COUNT          330 a          531 c          541 g          269 t
ORIGIN      
        1 aagcgaggag aatcacgtga ggaggggcga ccgatcccgg cggaaccgtg cgctgtgtgg
       61 aaccttgaac ctgatttctc aggtgggtgc cgcagacatg tcgcacacca cggtgtacgc
      121 aagagcagct cccggctcac aaagcgttgg ctcaagaccg catatgcaac acggaaaccg
      181 ccgggccggc cgagcgccga gcgtaccacg ttccgccggc gaaagtctat ccctcgcgtc
      241 ccgtcggtcc gtctcccgtt cccggtcgtt tccgcaagcg catcgaagag caccagcgcc
      301 gaatcgttga agctggcagg gatcgcgcca acgttcaata ccggcgacgc tcaccccccg
      361 acgccgcgta gaatagccag atacagattc gcatcgtgca aagaggcgat cgaatagttg
      421 ccgcgctcgc cttccaggag taccaagatc gatgagtgac atcgaccgcc cacgtcgccg
      481 ggtcgtcgtg accgggaccg gcgccatcag tgctgtcgga aataccgcgt cggaattgtg
      541 ggattccgtc cgaaatggac gatcgggaat cggcccgatt acccggttcg atcccgtgga
      601 gttcgagacc aggttcgccg gggaggtaaa ggattttgat ccggcgtcca ccatcggccg
      661 gaaagaatat cgacgccttg accgctatgc gcagttcgcg atcgtcgcga cacgcgaggc
      721 ggtgacccag tcaggcatcg acgtcgccgc ggatccgaca cgggtcgcgg tgttgatcgg
      781 caccggcatg ggcggcatgg agacgttcga atccggcacc gaaacgatgc tgacccaggg
      841 cgcgaaacgc gtctctccgt tcttcgtgcc gatgatgctg cccaatatgg gatcgggaaa
      901 tgtcgcgatc gacatcggcg ccaggggacc gaacttcgca acggtctccg cctgcgccag
      961 ttccggtcac gcgatcggcg aaggggtccg gatgattcgc gatggccgtg ccgacatcgt
     1021 cgtcgcgggc ggcgcggagg cgccggtcac ccggatcggg atcggcggct tcaacgcgat
     1081 gggcgcgctc tcgacgcgga acgacgatcc actcgcggcc agccggccgt tcgacgcgaa
     1141 ccgcgacggc tttgtcctgg cggaaggcgc agcgaccgtc atcctcgagg atcgtgatta
     1201 tgcgattgct cgcggtgcga cgatcctcgg cgagatcctc ggctacgcga cgacggacga
     1261 cgccaatcac atggtgcagc cgggccccgg cggtgagggg attgcccgcg caatggtcct
     1321 ggcgctgaag gatgccggca tcgtcgccga ggatctcgac tacgtgaatg cccacgggac
     1381 atccacccag ctgaacgaga agttcgagac ccagggctac aagcgggcgc tcggagatca
     1441 cgcctaccgc gtgccgatca gttcgacgaa atcgatgacc gggcatctcc tcggcgcggc
     1501 gggagcggtc gaggccgtga tcaccatgaa cgccctcaat gacggcctcc tgcctccgac
     1561 gatcaatcag accgacgacg acccggaatg cgacctcgac tacatcccga acactgcgcg
     1621 caaggcggat gcacgaatcg cgctctcgaa ttcgatggga ttcggtgggc a
//
LOCUS       DASTMD010000640         3448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2559709, whole genome shotgun sequence.
ACCESSION   DASTMD010000640 DASTMD010000000
VERSION     DASTMD010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2559709"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="VFQ54_09450"
     CDS             <1..1172
                     /locus_tag="VFQ54_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEU0165261.1"
                     /translation="AISLIAPVAIILVAENTGHIKAVSAMTGRNLMPFLGRGFIGDGV
                     ATTIAGLGGGTGVTTYAENIGVMAVTRVFSTAIFVIAAGTAIVLGFIPKFGALIGSIP
                     AGVLGGVSTVLFGLITITGVRIWVENQVDFSKATNLFIAGVTVIIGAADYTLNIGDFA
                     LGGIGLGTFGAIILYQVFRYAGLENVDLIPATDEVAGPPRSARPRNPNGGGRGRDQRD
                     RRPVRGQDRGAPRERVRDGDPGAGRGPRSEPTMSLDPRALREAFGGDTPPRRQAADDP
                     QRRPVRPSQAAPARDRAPQPRRRPEPYFDDELEDDFIEPSPQPRPQRQPAPDPRNVAP
                     QRRPAPPAAQPRRDVADDRPRRPAPQQPPQGDPRLRRPAPRPRTNQPPDDEDGDY"
     gene            complement(1556..2461)
                     /locus_tag="VFQ54_09455"
     CDS             complement(1556..2461)
                     /locus_tag="VFQ54_09455"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0165262.1"
                     /translation="MTGVFPIAPTPFHDDESIDLDGQARVIEFLIDAGVDGICILANY
                     SEQFALTDDERQRLQTTILRQVDGRVPVIVTTSHYTTRIAADRSRRAQDEGASMVMLM
                     PPYHGATLRIAESQITDYFRTIADQIEIPIMIQDAPMSGTPLSADILAALAREIPSVQ
                     YVKVESANAAAKLKRLIDLAGDSLPGPFDGEESVTLIPDLEAGATGTMPSSAIPEVLG
                     EIVRDWLVGDREKATSTWESWLPMIHYENRQCNLRGTKILMREGGIIGSARTRDPFGS
                     LPPEIAAGYIAHARRRDPLVLRWAR"
     gene            2813..>3448
                     /locus_tag="VFQ54_09460"
     CDS             2813..>3448
                     /locus_tag="VFQ54_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEU0165263.1"
                     /translation="MPTPTIDTGNTAWTLAAAALVMLMTPALGFFYGGLVNRKNVLST
                     LMHSFFILALISIVWVLWGYTLAFGPDKGGIIGGLDNIGLKDISPTTIYAGTNIPQYA
                     FVAFQMMFAVITPALISGAFAERKSFKAFVLFSLLWSTIVYSLLAHWVWGGGWLYKVG
                     AIDFAGGTVIHITSGVAALVTAIVLGKRKSFGTADADPHNLTLTVLGAGLLW"
BASE COUNT          553 a         1109 c         1126 g          660 t
ORIGIN      
        1 acgcgatctc cctgatcgcc ccggtcgcga tcatcctcgt tgccgagaac accggtcaca
       61 tcaaggccgt ctcggcgatg accggacgta acctgatgcc cttcctcggt cgcggcttca
      121 tcggtgacgg cgtcgcgacc acgatcgccg ggcttggcgg cggcacgggt gtcacgacct
      181 acgccgagaa catcggcgtg atggcggtga cccgcgtctt ctcgacggcg atcttcgtga
      241 tcgcggcggg aacggcgatc gtcctcggct tcatcccgaa gttcggtgcg ctgatcgggt
      301 cgatccccgc cggtgtgctc gggggcgtct ccaccgtcct cttcggtctg atcacgatta
      361 ccggtgtgcg gatctgggtc gagaaccagg tcgacttttc gaaggcgacg aatctcttca
      421 tcgccggtgt gaccgtgatc atcggcgccg ccgactacac gctgaatatc ggagatttcg
      481 cgctgggtgg gatcggcctc ggcacatttg gcgcgatcat tctctaccag gtcttccgtt
      541 acgccggtct cgagaatgtc gacctgatcc cggcgaccga tgaggttgcc gggcctccgc
      601 gcagcgcgcg tccccggaac ccgaacggtg gcgggcgtgg acgcgaccag cgcgatcgga
      661 gaccggtgcg cggtcaggat cggggtgcgc cgcgagagcg cgtgcgcgat ggcgatcccg
      721 gcgcaggccg tggcccgcgt tcggaaccga cgatgtcgct cgatccgcgt gcgctgcgag
      781 aggcgttcgg tggcgatacg ccgccccggc gtcaggccgc ggacgatcca cagcgccgac
      841 cggtgcgtcc gtcacaggcg gctccggccc gcgatcgtgc cccgcaaccg cgtcgccgac
      901 ccgagccgta tttcgacgac gaactcgaag atgacttcat cgagccgtct ccccagccgc
      961 ggccgcagcg tcagcccgcg ccggatccgc ggaatgtggc gccgcagcgc cgacccgcgc
     1021 caccagcggc acagcctcgt cgcgatgtag cagacgaccg gccgcgccgg ccggcgccac
     1081 agcaaccgcc tcagggcgat cctcgcttgc ggcgacccgc cccgcggcca cggacgaacc
     1141 agccgcccga cgacgaggac ggcgactact agatcgcgcc agggtccgtc gcgccaggtt
     1201 ccggtcgcat ggacggcgcc gtttgcggca agacacaagg tcttgcccta caagcccggg
     1261 ctccggcgaa cgtggtgcca gtcttgtggg tcatgtgccc atccaacccg actccggggg
     1321 ggtgcctccc ccgttggcga cggatgcctc cggcacgtga ccgccctccg cggggacgac
     1381 gccttcgacg gctcgtcgta cgggcggcgc ctttcgcggc aagacacacg gtcttgccct
     1441 acagacccgg gttcgaccaa cgtggtgcgg aacggcggga cgccgggagg cgggagagtg
     1501 aaccggcgcg acgttcggtc gacatcgagc ctcctggaaa cggatcgacg ggagatcacc
     1561 gcgcccagcg cagcacgagc ggatcgcgcc ggcgggcgtg ggcgatgtat ccggcggcga
     1621 tctcgggcgg cagcgacccg aaaggatcgc gcgtcctggc gctgccgatg atgccgcctt
     1681 cccgcatcag gattttcgtg ccgcgcagat tgcactgccg gttctcgtaa tgaatcatcg
     1741 gcagccacga ttcccaggtt gaggtcgctt tctcgcggtc accgacaagc cagtcgcgga
     1801 cgatctcgcc aaggacttcc gggatcgcgc tgctcggcat ggtgccggtc gcacccgcct
     1861 cgagatcggg gatcagggtg acgctctctt caccgtcgaa tggacccggc agcgaatcgc
     1921 cggcgaggtc gatcaggcgc ttgagcttgg cggcggcgtt cgcggattcc accttcacgt
     1981 attggaccga ggggatctcc cgcgccagcg ccgcgagaat atccgccgag aggggcgtgc
     2041 cgctcatcgg cgcgtcctgg atcatgatcg ggatctcgat ctggtcggcg atggtcctga
     2101 agtagtccgt gatttgcgat tcggcgatcc ggagtgtggc gccgtgatag ggcggcatca
     2161 gcatcaccat cgacgcaccc tcgtcctgcg cgcgccggct gcggtcggcg gcgatgcgtg
     2221 tggtgtagtg gctcgtcgtc acgatcaccg ggacgcgacc atcgacctgg cggaggatgg
     2281 ttgtctgcag gcgctgtcgc tcgtcgtccg tcagcgcgaa ctgctcggag tagtttgcga
     2341 ggatgcagat gccgtcgacc ccggcgtcga tcaggaattc gatgacccgg gcctggccgt
     2401 cgaggtcgat cgattcgtcg tcatggaacg gagtcggagc gatcgggaag actcctgtca
     2461 aggggttcgg gcatgccgat atcgacccgt cagattgtcc gttctgtgcg cacataccat
     2521 ctccttgtga cgaatgcccg ttttcacgtg tcgattactg cgctcagcat aacaagcggg
     2581 cagtgggacg tatgacgtcg ttaacaagtg attccgaaaa ctagtccatg tgcatagttt
     2641 ggggccgtcg atttaggttt tatgcacctt tgcggaattc gcgctgcttc atagactcta
     2701 tctattgcag cacgatgacg tgcgaaattg tgcatagtgt gcacccccta ttgcgtcgtg
     2761 tggggcgaga cgcaattctg gaccctttac tctcggaaga ggggtgactc gtatgccgac
     2821 accgacaatc gataccggga atacggcatg gacattggcg gcggccgcgc tcgtcatgct
     2881 catgactccc gcattgggat tcttctatgg aggtctggtt aatcggaaga acgttctctc
     2941 gaccctcatg catagcttct tcattctcgc cttgatcagc atcgtctggg tgttgtgggg
     3001 ctatacgctg gcgttcggtc cggacaaggg cgggatcatc ggcgggctcg acaacatcgg
     3061 cctgaaggac atctcaccta ctaccattta tgcgggcacg aatatcccgc agtacgcatt
     3121 cgtcgccttc cagatgatgt tcgccgtgat cacaccggcg ctcatctccg gcgcctttgc
     3181 cgagcgcaag agcttcaagg cgttcgtgct cttcagcctc ctctggtcga cgatcgtcta
     3241 ctccctcctc gcccattggg tctggggtgg tggctggctc tacaaggtcg gcgcgatcga
     3301 cttcgcgggc ggaaccgtca ttcacatcac gtccggcgtt gccgccctcg tgaccgcgat
     3361 cgtgctcggc aagcggaagt cgttcggtac cgcggatgcg gatccgcaca acctgacgtt
     3421 gacggtgctt ggcgccggtc tcctctgg
//
LOCUS       DASTMD010000641         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1577476, whole genome shotgun sequence.
ACCESSION   DASTMD010000641 DASTMD010000000
VERSION     DASTMD010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577476"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(775..>3597)
                     /locus_tag="VFQ54_09465"
     CDS             complement(775..>3597)
                     /locus_tag="VFQ54_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEU0165264.1"
                     /translation="TNVNYPFPVDPPFVPDDNPVGCYRHQFELPLSWDGRRLYLTFDG
                     VCSAFTVWLNGEEVGFSKGSHVPAEFEITSFVREGANQLAVQVLQWSDASYLEDQDMW
                     RLNGIFRDVWLLARNPVHIQDLHIETELADDFQNARLKIRASFASPEALPVQIQWTLL
                     TPSGDVARYWIDNLDGDPNGIGAVGEVADLPSPLLWSAETPNLYTLLVEHRSGDGELR
                     EILRQPVGFRKISVHDQQLWVNGVSIKIQGVNRHDDHPDFGYAVPYTAMVEDVRLMKQ
                     HNVNTVRTSHYPNDSRFYELCDRYGLYVIDEADLETHGFGPVGNWSQLANDPAWTDAF
                     LDRAQRMVQRDKNHPSIIIWSLGNESGYGDNHDAMAEWIRAYDTTRPTHYEGSVHIAD
                     RVPRETSFHSVMYPSVATVIAEGQRTDDPRPYFMCEYAHAMGNGPGSFKEYWEAIRAH
                     ERLIGGCVWEWADHGIRQRRDSGEEFFAYGGDFEDFPNDGNFCIDGLFSPDRVPHPAA
                     IEMKKIYEPVEADLVDAHDGIVRIGNRRAFAGLDDLAVRWELGANGRLIQQGTLSTLE
                     IPAGTSALVAVPYTVPETSPAIDYWLDIFFTTAGASPWAPAGFEVAHIQLGLLLAADR
                     VSPLAATPVPPVVAVENEELIYVHTENGDVIFDREVGAIVDWSLNGRGLITSGPRLNI
                     WRAPIDNDRYMEATWREFGLDKLQHRIERCELVAEDETSVAIEVEAMVGAYSRRPAFH
                     VHYLYVITGDGEVAITTGVNPLPSMDDLLTLPRVGLQFVMPDTFDRVTWYGLGPHESY
                     SDRRESVTVGEWSGTVEEQFENYTFPQENGGKAETRWAAISSTHGMGILAIGEEPLQF
                     SASHFTPEDLDAAKHTYDLIPRDETIVNLDHLHVGLGSSICGPRPLDQYLVPPVETTF
                     TIRLRPIASTQGIGDLAAAVRSSVVV"
BASE COUNT          632 a         1104 c         1228 g          633 t
ORIGIN      
        1 gtcggcatcg ggcacgatcc cgcggttacg aagaagcatc cggcttgccg cgctcgtcaa
       61 gcatcgtctc cctccgtcat ccttaacagg gcgtcaagat tcacgtgtcc agcagcgcac
      121 atgccggggg acccgtcccc gcggcccctg tggccgcctc cgagtcggcg gcgctgatcc
      181 gcttcccggg aaccctgctc ggagacggtc gcgtcggagc ccgccggtgg cgggtgcgct
      241 tcgcgcgggg gtccttcgct ccgctaagga cggtggatgc gttggaaccg cccaagccca
      301 cgggaatgga cgcccgtcac cctgaacccg tcggaaaccc gtttgacgct ccactgaatg
      361 aggagaatga cctcccggtg cagatcgact tcaaccccgg ccttggatgc gacgcgtttc
      421 gaaggacgac gcggagccga gacgggtcgt ctggaggctc gttgaggcgg gacaggccgt
      481 ccggcccttc ggtccggcag cggcggtccc gccctacgaa cgaccgcggc ctcaacgatc
      541 gcgcaggatg ttcgccatgc cgggaatgcg cctggaaacg ccgggttttc caggcgcggg
      601 cgttcatcgt cgccgcgaac acggacgatc cattacggcg tgcggggcgc cgacggtccg
      661 ggccgcgatg gccgcacgtg tcgcacctaa ggagttccgg aagatcctcc gcggtgcggt
      721 aacggtcaga cgagctggcc cgctacggag cgatgccggg gccgaagcga gacactatac
      781 gactacggaa ctacggaccg cggcggcgag gtcgccgatt ccctgcgttg aggcgatcgg
      841 tcggaggcgg atcgtgaagg tggtctcgac gggcgggacc aggtactggt cgagcggtcg
      901 cggaccgcag atgctgcttc ccaatccgac gtgaaggtga tcgagattca cgatcgtctc
      961 gtcccgcggg atcaggtcat acgtgtgctt cgcggcgtcg agatcctccg gggtgaagtg
     1021 ggatgcggag aactggagcg gctcctcgcc gatcgcgagg atgcccatcc catgcgtcga
     1081 cgagatcgcg gcccatcgcg tctcggcctt gccgccgttc tcctgcggga aggtgtagtt
     1141 ctcgaattgc tcttcgacgg tgccgctcca ctcgccgacg gtgacgctct cccgtcgatc
     1201 ggagtagctc tcgtgcggac caagtccgta ccaggtgacg cgatcgaatg tgtccggcat
     1261 cacgaactgc aacccgacgc ggggcaacgt caggagatcg tccatcgacg gcagggggtt
     1321 gactccagtc gtgattgcga cctcgccatc gccggtgatt acgtagagat agtggacgtg
     1381 gaacgccggt cgtcgcgagt aggcgccgac catcgcctcg acttcgatcg cgaccgaggt
     1441 ctcatcctcg gcgaccagtt cgcagcgctc gatgcggtgc tggagcttgt ccagcccgaa
     1501 ctcccgccac gtcgcctcca tgtagcggtc gttgtcgatc ggcgcgcgcc agatgttgag
     1561 tcggggaccg ctggtgatca gaccgcggcc attgagcgac caatcgacga tcgcgccgac
     1621 ctcacgatcg aagatgacat cgccattctc ggtatgaacg tagatgagct cctcgttctc
     1681 caccgcgacg accggtggca ccggggtggc cgccaaggga gagacccggt cggcggcgag
     1741 cagcagacca agctggatgt gggcgacctc gaagccggcg ggcgcccagg gcgatgcccc
     1801 ggcggtcgtg aagaagatgt cgagccagta atcgatggcc ggggacgtct ccggcactgt
     1861 atacggaaca gcgacgagcg ccgatgttcc ggcaggaatc tcgagcgtcg acaacgttcc
     1921 ctgctggatg aggcgaccgt tcgcgccaag ttcccagcgg acggcgagat cgtcgagacc
     1981 ggcgaaggcg cgccggttgc cgatccggac gatcccgtcg tgggcgtcga cgaggtcggc
     2041 ctcgaccggt tcgtagatct tcttcatctc gatcgccgcc gggtgtggca cgcgatccgg
     2101 cgagaagagc ccgtcgatgc agaagtttcc gtcgttgggg aaatcctcga agtcgccgcc
     2161 gtaggcgaag aattcctccc cggagtcgcg acgctggcgg atgccatgat ccgcccactc
     2221 ccagacgcag ccgccgatca ggcgttcgtg ggcgcgaatc gcctcccagt actccttgaa
     2281 gctccccggg ccatttccca tcgcatgcgc gtactcgcac atgaagtagg ggcgtgggtc
     2341 gtcggttcgc tgcccctcgg cgatcacggt ggcgacggat ggatacatca cgctgtggaa
     2401 gctcgtctcg cgtgggacgc ggtcggcgat gtggacgctg ccttcgtagt gggtggggcg
     2461 cgtcgtgtcg taggcgcgga tccattcggc catcgcgtcg tggttgtcgc cgtaaccgga
     2521 ttcgttaccg agcgaccaga tgatgatcga gggatggttc ttgtcgcgct ggaccatccg
     2581 ctgggcgcga tcgaggaagg catcggtcca ggcgggatcg ttcgcgagtt gcgaccagtt
     2641 gccaaccggg ccgaaaccgt gcgtctcgag gtcagcctcg tcgatgacat acaggccgta
     2701 gcggtcgcag agttcgtaga agcggctgtc gttcggatag tgcgaggtgc gaacggtatt
     2761 gacgttgtgc tgcttcatca gccggacatc ctcgaccata gcggtgtacg gaaccgcgta
     2821 gccgaaatcc ggatgatcgt cgtggcgatt gacgccctga atcttgatcg agacgccgtt
     2881 gacccagagc tgttggtcgt ggacggagat cttccggaac ccgaccggct gacgaaggat
     2941 ctcccgcagc tcgccgtcac cgctgcggtg ctcgaccagg agcgtgtaaa gattcggcgt
     3001 ctccgccgac cagaggagcg gcgacggaag gtccgcgacc tcaccgacgg cgccaatgcc
     3061 gttcggatcg ccgtcgagat tgtcgatcca gtagcgggcg acgtcaccgc tcggggtgag
     3121 cagcgtccat tgaatctgga cgggaagcgc ctccggagac gcgaacgagg cgcgaatctt
     3181 caggcgtgcg ttctggaagt cgtccgcgag ttctgtctcg atgtggaggt cctggatgtg
     3241 gacggggttg cgcgcgagga gccagacatc gcgaaagatg ccgttcagtc gccacatgtc
     3301 ctgatcctcg aggtaggagg catccgacca ttgaaggacc tgcacggcga gttgattcgc
     3361 gccctcgcgg acgaagctcg tgatctcgaa ctcggcgggc acgtgactgc ccttgctgaa
     3421 gccgacttcc tcgccgttga gccagacggt aaaggccgag cagacaccgt cgaaggtgag
     3481 atagaggcgg cgaccgtccc acgaaagcgg cagctcgaat tgatggcgat agcaaccgac
     3541 gggattgtca tccggaacaa acgggggatc gaccgggaag gggtagttca cgttcgt
//
LOCUS       DASTMD010000642         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2827704, whole genome shotgun sequence.
ACCESSION   DASTMD010000642 DASTMD010000000
VERSION     DASTMD010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2827704"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFQ54_09470"
     CDS             <1..579
                     /locus_tag="VFQ54_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165265.1"
                     /translation="VESVRMTSPAAATVPVGTVVTGIDVDAASGVGLGTLVGGITVTT
                     GVKAGVGGTPVASAVGSCVMVAVAVGADDGGVLVDANVEIGFGVCVPVGAVVAVGVVV
                     GTGSCVVVAAGSGVDVVVGIGVSVAGAVSVSVGATVTVGTGVSVAVNVGVSVGVMVDV
                     GLGVNVALGVGVSVGVTVAVAVGTLGDPQVIV"
     gene            complement(1520..1592)
                     /locus_tag="VFQ54_09475"
     tRNA            complement(1520..1592)
                     /locus_tag="VFQ54_09475"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1557..1559),aa:Phe,seq:gaa)
     gene            complement(1630..1702)
                     /locus_tag="VFQ54_09480"
     tRNA            complement(1630..1702)
                     /locus_tag="VFQ54_09480"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1667..1669),aa:Val,seq:tac)
     gene            complement(1724..1811)
                     /locus_tag="VFQ54_09485"
     tRNA            complement(1724..1811)
                     /locus_tag="VFQ54_09485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1775..1777),aa:Leu,seq:gag)
BASE COUNT          338 a          567 c          710 g          391 t
ORIGIN      
        1 gtcgagagcg tcaggatgac gtcgcccgcc gccgcgaccg ttcccgttgg gaccgtggtg
       61 acggggatcg atgtcgacgc cgcgagcggc gtcgggctgg gcacactggt tggaggtatc
      121 acagtcacca cgggggtgaa ggccggagtt ggcggcacgc cggtggcatc cgccgtcggg
      181 tcttgtgtga tggtggccgt ggcggtcggg gctgacgatg gcggcgtgct cgttgacgcg
      241 aacgtcgaga tcgggtttgg cgtctgcgtc ccggtgggcg cggttgtcgc cgtcggggtg
      301 gtcgtgggga ccggctcctg cgtcgtcgtc gcggcaggaa gtggagtgga cgtcgttgtc
      361 gggatcggtg tgagcgtagc gggcgccgtg agtgtcagcg tcggagcgac ggtgaccgtt
      421 gggacgggcg tcagcgttgc ggtcaacgta ggtgtcagcg tcggagtgat ggtggacgtt
      481 gggctcggcg tcaacgttgc gctcggcgtg ggtgtcagtg tcggagtgac cgtggctgtc
      541 gcggtgggga cgctcgggga tccccaggtg atcgtatagc atgggctaac gaccgtggga
      601 tccgagagga gacttgcctg gaccgaggca gtttcgtccg acccctggaa gccggatgcc
      661 cctccgggcg ggatgtggtc gtactgcggc acgccagagc acgatgttcc cgcccagccc
      721 gtcatctcgg cattggaatc tgtatcgaga tggacgccat attcgttgat gacgctgatc
      781 gacccggcaa ccccatttag cgtgagatgg ggtgtcgtcg cgggttgcgg ttcccaggtg
      841 atggaataac aggggctcac cgtcgccgga tctgatatca ggctcgcgcg agcggatgcg
      901 gtttcattcg atccttgtgc tgcctgcccc gaagcgactt cggtttcctg cggggtaccc
      961 gagcacgttg cgcccgtcca cgccgttatc tcggcatcgg catcggtgtt gagggtgacg
     1021 aaagaaccgt tcacgacggt aatcggtcca acgaccccat tgaggttcag ggagggcggc
     1081 gtcgcggccg aggtgattcc tggttcgatg agcaatccca tgaatgcggt caggacgatc
     1141 gagacatgta gcccggcgac ggcagcacgg cgaacgcgag atgtcatgaa ccaccacctt
     1201 cttgtagtcc gagctacgag atcaatgtgg ccccgaggac gtgatttcac ggatacgata
     1261 cgggacaccg gatcgctcgg caatgcttct ggttcgggag ggctcgtgcc ccggaggggc
     1321 agctattgcg ccacatcgca cgagacttgc gaggatgtgt cggtgggact ttctctgctg
     1381 gctggctcag cccgcgaccg ggattgcttg ccgtgaggga gacgccggag gggtccgcgc
     1441 gccgcggacc cggcgcgagc gcaagcggtc acaaaaaacc cgaaatccct gggaaggcca
     1501 gggacgtcgg ggatctcggt gccaagggcc gggctcgaac cggcgaccgc acgattttca
     1561 gtcgtgtgct ctaccaactg agctaccttg gcatcatctt ggaagtcgaa acgcgcgcga
     1621 caccaccggt gggcgataag ggactcgaac ccctgacatc cacggtgtaa acgtggcgct
     1681 ctaccagctg agctaatcgc ccaagtagcc ggattgttcc aactggtgcc caggagagga
     1741 tttgaacctc cacgccctaa acgggcacta gcccctcaag ctagcgcgtc tgccgattcc
     1801 gccacctggg caggtgcgtc gttgccgcag cgggcgaagt ataggctgat tcaaacgcca
     1861 tcgtcaatgc tccgctccga cgaaactccg gaggtcgttt tggctgattc gcccgacgtt
     1921 tctggcatct ggtggctacc cggaagggag gtctcccgcc gtccgtggta gaacatccat
     1981 gaatgtctcc tgttgccctc gtgtaa
//
LOCUS       DASTMD010000643         2550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_683817, whole genome shotgun sequence.
ACCESSION   DASTMD010000643 DASTMD010000000
VERSION     DASTMD010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683817"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..2371)
                     /locus_tag="VFQ54_09490"
     CDS             complement(53..2371)
                     /locus_tag="VFQ54_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEU0165266.1"
                     /translation="MQRTLTSQIRLRTRSETTRLTHLPKGPLQRAARIAIATTAAFAI
                     VDHISPIAKSASFAVFGCIAFLLFANLGPGTRRQHAGLHGALVVAGGALLALGTACSG
                     NVWLAAIVGTAVSFAVLLSGLIGPVFAGLTLPMLVSFVIGATVPGPISAIPDRLWAWL
                     IAGAVALVAVVLPAAARPDRALRELAAETSRQLGVFLRHEGNGDEAASTTQADLFSAT
                     SGTVARLRSTFYGMQYSPSGLGADGRALIESVQQLVLFGRALDRIAARPGNPLADVAI
                     RPVVLAAANVLEHCATILVATGTSFADLEESLDQLRVAGEAIEAEALSLASGQIAPPP
                     HDDDLLVLLEPGFRAREIVLAVTAIARSTRSSAEMHRRSWWHHLLDRSPLTPPSPGSL
                     NAWIRRSIAHIDRHAVWLQNSVRGSLGLGIAVFVANTSGVDRAFWVAFGTLSVLRSTA
                     TNTGQSVLRALAGTIVGSVIGGLLVTLIGSSSPLAWVLFFLALGFSSLAPAVVSFGAG
                     QAGFSVAVIMLFSLVAPISWSISLVRIQDVAIGCGVALLMSLLFWPRGASQALGAALS
                     EAIFESSRYLADSTRFAIAQCDERTNPVADEGRPQPYRATAAARRLDDAFRQFLSERG
                     TKGITLAEITTLVTTTAILREVADDICELWVRPGYVHRADHTAIREAILRFEQPITGW
                     FTDLALSLIGYSRLPIAPDIEDEAELSRAAIIQRDPDPDGHTGGSTSVKLVWTLEYLA
                     TVQRLEAGLLRSAQIASSALRPPTGRDMVRGY"
BASE COUNT          434 a          832 c          899 g          385 t
ORIGIN      
        1 ctcggctcct cggctcctcg gctcctcgga ccgcctgtcc cgtctgcacg tctcagtagc
       61 cccgcaccat gtcccgtccc gtcggtggtc gcagcgccga cgaggcgatc tgcgcggatc
      121 gcagcaggcc agcttccagc cgttgaacgg tggcgagata ctccagcgtc cagaccagct
      181 tgaccgaggt cgatcctccc gtgtggccgt ccggatctgg atcgcgctgg atgatcgccg
      241 ctcgcgagag ttcggcctcg tcctcgatgt ccggtgcaat cgggagccgg gagtatccga
      301 tcagcgagag ggcgagatcg gtgaaccacc ccgtgatcgg ttgttcgaag cgaaggatcg
      361 cctcgcgaat cgcggtgtga tcggcacgat gcacgtagcc cggtcgcacc cagagttcgc
      421 agatgtcgtc ggcgacttcg cgcaggatgg cggtcgtcgt gacgagcgtc gtgatctcgg
      481 cgagcgttat ccccttcgtc ccgcgctcgc tgaggaactg gcggaacgcg tcgtccagtc
      541 tccgtgccgc ggcggtcgcg cgatagggtt gcgggcgacc ctcgtccgcg accggattgg
      601 tgcgctcgtc gcactgggcg atggcgaagc gggtcgaatc cgcgagatag cgtgacgact
      661 cgaagatcgc ctccgagagc gccgcgccga gcgcctgact cgcgcctcgc ggccagaaga
      721 gcaggctcat cagcagcgcc acgccgcatc cgatcgccac atcctggatg cggacgaggc
      781 tgatcgacca gctgatcggc gcgacgagac tgaacagcat gatgacggcg accgagaaac
      841 ccgcctgccc tgccccgaag gagacaaccg ccggcgcgag gctgctgaag ccgagcgcaa
      901 ggaagaagag cacccaggcg agtggcgacg acgatccgat gagcgtcacg agcagaccgc
      961 cgatgacact ccccacgatc gtccccgcca gcgcgcggag gacgctctga cccgtgttcg
     1021 tggcggtgga acggaggacg ctgagggtgc cgaaggcgac ccagaaggcg cggtcgacac
     1081 cgctggtatt ggcgacaaag acggcgatgc cgagaccgag ggaaccccgc acgctgttct
     1141 ggagccagac cgcatgccga tcgatgtggg cgatgctccg ccggatccag gcattgagcg
     1201 aacccggaga cggcggcgtc agcggcgagc gatcgagcag gtgatgccac cacgagcggc
     1261 ggtgcatctc cgccgaagac cgggtcgacc tggcgatcgc cgtcaccgcg aggacgatct
     1321 cacgggcgcg gaagccgggc tcgaggagga cgaggagatc gtcgtcgtgc ggcggcggtg
     1381 cgatctgtcc cgaggcgagg gagagcgcct ctgcctcgat tgcctcgccg gccacccgga
     1441 gctgatcgag actctcctcg agatcggcga aggacgtccc cgtcgcgacg aggatcgtcg
     1501 cgcagtgctc gaggacattc gccgccgcga ggacgaccgg acggatcgcg acatccgcca
     1561 gcgggttccc gggtcgagcg gcgatccggt ccagcgcacg tccgaacagc acgagctgct
     1621 ggacggattc gatcagtgcc cgcccgtcgg cgccgagtcc ggatgggctg tactgcatgc
     1681 cgtagaaggt cgagcgcagg cgggcgaccg ttcccgatgt cgcgctgaac aggtcggctt
     1741 gcgtcgtact cgctgcttcg tcgccattcc cttcgtggcg caggaagaca ccgagttgcc
     1801 gggatgtctc cgccgcgagc tcgcggagcg cgcgatccgg tcgagccgcc gcgggaagga
     1861 cgaccgctac cagtgcgacg gcaccggcga tcagccatgc ccacaagcga tcggggattg
     1921 cactgatcgg cccgggcacg gtcgccccga tcacgaagct gacgagcatc ggcagggtca
     1981 gaccggcgaa gaccggtccg atcaatcccg agagaaggac ggcaaagctg accgctgtgc
     2041 cgacgatcgc cgccagccag acgttgcccg agcaggcggt tccgagcgcg aggagcgccc
     2101 cgccggcgac gaccagtgcg ccatggagcc cggcgtgttg gcgccgtgtg cccggtccga
     2161 ggttggcgaa gaggaggaag gcgatgcagc cgaagacggc gaatgacgcg ctcttcgcga
     2221 tcggcgagat gtggtcgacg atcgcgaagg cggcggtggt cgcaatcgcg atccgggcgg
     2281 cgcgttgcag cggccccttc ggcaggtggg tcagccgggt ggtctcgctt ctcgttcgca
     2341 gccggatctg actcgtcagt gtgcgttgca cggtctcacc tgcttccggc tacgagcacg
     2401 ccatcgcggg gaatcggctc cctgcgtgca agactgtggt cgatggtaca tcccgcccgg
     2461 caattcgtcg ctctgccgta tgaatcagac gcaaccctgc tgtcacgttc gcgacagcgg
     2521 ggctgtgtac gtggcgtctc attttggcgg
//
LOCUS       DASTMD010000644         1612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_596659, whole genome shotgun sequence.
ACCESSION   DASTMD010000644 DASTMD010000000
VERSION     DASTMD010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596659"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1612)
                     /locus_tag="VFQ54_09495"
     CDS             complement(<1..>1612)
                     /locus_tag="VFQ54_09495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0165267.1"
                     /translation="RSGRAITIEDLYEFVLPGEPNVSPDGSRVVAAVSSVSREDNGYR
                     AGLWLVPLDGSPARRLTGGKWSDTSPRWSPDGTRIAFLSNRTEKTNNIWILPTDGGEA
                     HQVTTLKNGAGEIGWAPDGQSLVFTSRVDPESATRGSDEETKDGDEKSDVKVITSARY
                     KFDGRGFLEDKVGHIFTIDVANEDAEPVQLTSGHFNHTSPAWSPTGTEIAFVANRDPN
                     WDLNGIADIWTIPASGGDPLRVTNGGGQFANPAWSPDGTRLAFTGERELSPIHVNAQL
                     WISSATGEDLHSISADVDRSIGDSSMSGPGGPVTGSLIQWTPDGRALDALVGDRGQTV
                     VVRFPLDASGATQISPSGRHIAAFAVAGDDLILTAADATTPFEFRKISAGSETALTSF
                     NTAWTAEVAIASPEEFWVESNGEAIQGWLLRPAGNSATSDPVPVVLNVHGGPHGQFSN
                     AFTHELQLYAAKGYALVYVNPRGSVGYGEDFAQQVARNWGEADTPDFLAAIDHALALG
                     GLDATRVGVTGGSYGGFTTNWLLGHSDRF"
BASE COUNT          260 a          512 c          555 g          285 t
ORIGIN      
        1 gaaccggtcg ctgtgcccga gtagccagtt cgtcgtgaag ccgccgtagg agccgccggt
       61 gacaccgacc cgggtcgcgt cgagaccgcc gagcgcgagc gcgtgatcga tcgcggcgag
      121 gaagtccggg gtatcggctt caccccagtt ccgggcgacc tgctgcgcga aatcctcgcc
      181 gtagccgaca ctgccccgcg ggttgacata gaccagtgcg tagcccttcg cggcatagag
      241 ttgaagctcg tgcgtaaagg cgttggagaa ttgcccgtgc ggtccaccgt ggacgttgag
      301 gaccaccgga accgggtcgc ttgtcgccga gtttcccgcc gggcgaagca gccagccctg
      361 gatcgcctcg ccgttcgact cgacccagaa ttcctcgggg gaggcgatcg cgacttccgc
      421 cgtccatgcc gtgttgaagg aagtcagcgc ggtctcactg ccggcggaga tcttccggaa
      481 ttcgaacggc gtcgtcgcgt cggcagcggt gaggatcagg tcgtcaccgg cgacggcgaa
      541 tgcggcgatg tggcgaccgg acggtgagat ctgggtcgcg ccggatgcgt cgagcgggaa
      601 gcgaacgacg accgtctgcc cgcgatcgcc gaccagggcg tcgagcgcgc gaccatcggg
      661 agtccattgg atcagcgagc cggtgaccgg tccgcccggg ccactcatgc tggaatcacc
      721 gatcgagcga tcgacatccg ccgagatcga gtggagatcc tcgccggttg ccgacgagat
      781 ccagagctgg gcgttgacgt ggatcggcga caactcgcgc tcaccggtga aggcgagtct
      841 ggtgccgtct ggtgaccagg ccgggttcgc gaactggccg ccaccattcg tgacgcgaag
      901 cggatcgccg ccgctcgcgg gaattgtcca gatgtcggcg atgccgttga gatcccagtt
      961 cggatcgcgg ttcgccacga aggcgatctc cgttccggtg ggtgaccagg ctggacttgt
     1021 gtggttgaaa tggccgctcg tcagctgaac cggctcggca tcctcgttcg cgacgtcgat
     1081 cgtgaagata tgaccaacct tgtcttcgag gaagccacga ccgtcgaact tgtagcgggc
     1141 cgacgtgatg accttgacgt cgctcttctc gtcaccgtcc ttcgtctcct cgtcgctccc
     1201 cctggtggcg gattcgggat cgacgcgact cgtgaagacc agcgactggc cgtccggcgc
     1261 ccagccgatc tcaccggcgc cgttcttcag cgtcgtgacc tgatgggcct cgccaccgtc
     1321 cgtgggcagg atccagatgt tgttcgtttt ctcggtgcgg ttggagagga aggcgatccg
     1381 cgtgccgtcc ggcgaccagc gcggcgaggt atccgaccac ttgccgccgg tgaggcgtcg
     1441 cgcgggtgaa ccatcgagcg gaaccagcca gagcccggcc cggtagccgt tgtcttccct
     1501 gctgaccgag gagacggcag cgacgacgcg gctgccgtcc ggcgagacat tcggctcacc
     1561 cggcaggacg aattcgtaga ggtcctcgat cgtgattgcg cgtccgctgc gg
//
LOCUS       DASTMD010000645         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1399512, whole genome shotgun sequence.
ACCESSION   DASTMD010000645 DASTMD010000000
VERSION     DASTMD010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399512"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFQ54_09500"
     CDS             <1..256
                     /locus_tag="VFQ54_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165268.1"
                     /translation="NTSGIRQPPKESMPAAPIVALYTSMGGRQVTMGSDSHVAETIAF
                     GFDQTYEMLVAKGVDEISTFRDRERTNVPIQDLISRNQGA"
     gene            471..1331
                     /locus_tag="VFQ54_09505"
     CDS             471..1331
                     /locus_tag="VFQ54_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165269.1"
                     /translation="MNVQPIEQARTPRRAAVREPFVLPPARALLAEAVPSLVMAAAAL
                     GAALLGALEILNIPLWAFGMIVPPLLVFALADGVTQPLWKRASVIDLVVLLAIFPLLV
                     VRQSVARIPFMDTSNGTMMAPVLMTLILLIGLTGVAVGSAALCREDPEYSGVVFLPAA
                     MFVPIFAGATSITSFRTAMLMTATIYLATAILTMVASVAPRQWVIVFTPATLALEFLI
                     LTAVRGSDIFPIGAGFASKALFFFVVAYTVALAIAVPMLSYWVRHVSLIVHGAIAQEP
                     PPSRRAAASL"
     gene            complement(1701..>2477)
                     /locus_tag="VFQ54_09510"
     CDS             complement(1701..>2477)
                     /locus_tag="VFQ54_09510"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEU0165270.1"
                     /translation="LPDASRTAKGLPIVNVINMQPDETVTTLMKVRDYNLAKYLFFTT
                     RQGRVKRVALDQFKSVRSTGMIAIGIDADDELAWVRMTEGEDEMVLVTSGGQAIRFPE
                     GDVRPMGRTAAGVIGIRLDPGDRVIASDVVTPDKDLLVVSAKGLGKRTGLDQYRAQSR
                     GGKGIQAMRLTDRTGAIVAAGMAGPDDAIMLMNNKGIAIRIAASQISRIGRTTQGVTL
                     MRLGKTEEIVQMTVIEPKDPSAAARLNGLEDGEPEEESAS"
BASE COUNT          393 a          823 c          791 g          470 t
ORIGIN      
        1 caatacctcg gggattcgcc agccgccgaa ggaaagcatg ccagccgccc cgatcgtggc
       61 gctctacacg tcgatgggcg gtcgacaggt cacgatggga agtgattccc acgtggcgga
      121 gacgatcgca ttcggattcg accagaccta tgagatgctc gtcgccaagg gtgtcgacga
      181 gatatcgact ttccgcgacc gcgagcgcac gaacgtgccg attcaagacc tgatttcccg
      241 caatcaagga gcctgaacga accacgatgg gatcaaacag ccccgatcgc gagcagaacg
      301 gcttgccaga tctcccattc gatatgcaat cggtggatat ctatccccgg cgccgaccgc
      361 cgtcccggca gcaacgaccg gcgggagggc gcacgtcatc gccggcgcgt ggcagacgcg
      421 aaccgacgac acgcccccct cgcgtggcgc gtcaggtcga agacgaagtg atgaatgtgc
      481 aaccgatcga gcaggcgaga actccgcgcc gcgccgcggt ccgcgagccg ttcgtcctgc
      541 caccggcacg ggcgcttctc gccgaggctg tcccgagcct cgtgatggcg gcggcggcac
      601 tgggggcggc gttgctcggc gcgctcgaga tcctgaacat tccgctctgg gcgttcggga
      661 tgatcgtgcc gccgctcctc gtcttcgcgc tggccgacgg cgtcacccag ccgctctgga
      721 agcgggcttc ggtgatcgat ctcgtggtgt tgctcgcgat ctttccgctg ctcgtcgtcc
      781 gccagagtgt cgcgcgaatc cccttcatgg acacatcgaa cgggacgatg atggcgcccg
      841 tcctgatgac gctgatcctg ctgatcggcc tgaccggggt tgcggtggga agcgcggcgc
      901 tttgccgcga ggatccggaa tattcgggcg tcgtctttct tccggcggcg atgttcgtac
      961 cgatcttcgc cggagcgaca tcgattacga gctttcgcac cgcgatgctg atgacggcca
     1021 cgatctatct cgccacggcg atcctgacga tggtggcctc ggtcgctccg cgccagtggg
     1081 tgattgtctt caccccggcg acgctggcgc tcgaattcct catcctgacg gccgttcggg
     1141 gatcggacat cttcccgatc ggggccgggt tcgcatcgaa ggcccttttc ttcttcgtcg
     1201 tggcctacac ggtcgccctg gcgatcgcgg taccgatgct gagctattgg gtgcgacacg
     1261 tctcgttgat cgtccatggc gcgatcgccc aggagccgcc gccatcccgg cgcgctgctg
     1321 cctcgctgta gttcgctggt cgcaacgaga aaggctccgc tcgaaatcga gtggagcctt
     1381 tctcgttgca ccgggtcggg atcctggcgt cacgactgaa atgatcggca caggcgagcg
     1441 gcgacgcgtc tcccgcatcc gctagtgttt tcccggagtt cgctttgcgg cggggcataa
     1501 aaccccgccc taccaacccc agtcctgcca aacctgtcga cgattccacg ttacgccgca
     1561 gggaggaccg aaaggatcgc cgcacgtgcc ggcggaccgg atgcgacatt ctgcaccgca
     1621 tccggtcatt cgcggggccc gtcccgactc acggtcggcg gaggatcgtg ctccccgcgg
     1681 cagcgatttc gttcgccgcg tcaggaagcc gattcctctt cgggctcgcc atcttccagg
     1741 ccgttgaggc gggccgcggc ggacggatcc ttcggttcga tcaccgtcat ctgcacgatc
     1801 tcctcggtct tgccgaggcg catcaacgtc acgccctgcg tcgtgcgacc gattcgcgag
     1861 atctggcttg ccgcgatccg gatcgcgatg cccttgttgt tcatgagcat gatcgcgtca
     1921 tcggggccgg ccatgccggc agcgacgatg gcgcccgtgc ggtcggtcag gcgcatcgcc
     1981 tggattccct tgccaccgcg gctctgcgcc cggtactggt cgagccccgt ccgcttgccg
     2041 agtcccttcg ccgatacgac gaggaggtcc ttgtccggag tgacgacatc ggaggcgatg
     2101 acgcgatcgc ccggatcgag gcggatgccg atcacgcccg cggctgtccg gcccatcgga
     2161 cggacatcac cctcggggaa gcggatcgcc tggccgccag aggtgacgag caccatctcg
     2221 tcctcaccct cggtcatccg gacccacgcc agctcgtcgt ccgcatcgat cccgatcgcg
     2281 atcatgccgg tcgagcggac cgacttgaac tgatcgagtg ccacgcgctt gacgcgtccc
     2341 tgacgggtcg tgaagaagag gtacttggcg aggttgtaat cgcggacctt catcagcgtc
     2401 gtcacggttt cgtccggctg catgttgatg acattgacga ttggcaggcc cttggcggtc
     2461 cggctggcat cgggaag
//
LOCUS       DASTMD010000646         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3005322, whole genome shotgun sequence.
ACCESSION   DASTMD010000646 DASTMD010000000
VERSION     DASTMD010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3005322"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="VFQ54_09515"
     CDS             <1..1497
                     /locus_tag="VFQ54_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0165271.1"
                     /translation="GPRTDPATVTQLREAIAAAREVTVTLLNYRKDGTPFWNEVHISP
                     AFDAEGKLTHYVGVQSDVTERKRAEDNRNLLVEASGIIAATARPEDTIVQIARAAVPT
                     ISDICLGYLVDPNAPIDDPASPRLVAYESMTHVTDADRQRISELQPGVSDDLHGVGWV
                     LRTGETTWIPEPSPLDILSETPSPDTEPRAQRFVGKSLIIAPIASTARIHGALVFLID
                     QSNRRFQASDVHLARDLGRRIGALLDNISLYEEAQAAIRARDHFLSIAAHELRTPAVS
                     IKGYAQFLLRSLDRDTLSKERLRHALETVDASVLRLSTLTDDLLSVSRRNIDHLPWRR
                     EVINVGHYLVDFFSASGPAASKGYDVNLDVGHADVEVNVDTERLHQVLTILLGNAVKF
                     SPDHDEISVFAESDANGVTITISDLGIGLLPGEEDKIFEPFGRTDEAIKDKLSGLGIG
                     LFIARSIVERHQGRIWAESQGRNLGASFNVWLPAFRRPAVDHPEPPPA"
     regulatory      1941..2048
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT          452 a          787 c          663 g          430 t
ORIGIN      
        1 gggccccgca ccgatcccgc gaccgtcacc cagcttcgcg aggcgattgc cgccgccagg
       61 gaggtcacgg taacgcttct caactaccga aaggacggta ccccgttctg gaacgaagtc
      121 catatctctc ccgcattcga cgcggagggc aaactcaccc actacgtcgg cgtccagtct
      181 gatgtcaccg agcggaagcg cgccgaggac aatcgcaatc ttctggtaga ggcgagcggc
      241 atcatcgccg cgacggcgcg tcccgaagac acgatcgtgc agatcgctcg cgctgccgtg
      301 ccgacgatca gcgacatctg cctcggatat ctcgtcgatc cgaacgctcc gatcgacgac
      361 cccgcctcgc cgcgacttgt cgcctacgag agcatgacgc atgtcaccga cgccgatcgg
      421 caacgcatct ccgaacttca acccggcgtg agcgacgatc tgcatggcgt cggttgggtc
      481 ctgcgtaccg gcgagacaac ctggatcccc gaaccctcac cactcgatat cctctcggaa
      541 acgccatcgc ccgatacgga accccgggcc cagcgttttg tcggcaagtc gttgatcatc
      601 gccccgatcg cctcgacggc gcggatccat ggagcgttgg tcttcctgat cgaccaatcg
      661 aaccgtcggt tccaggcttc cgatgtgcat ctcgcacgcg acctcggacg ccgcatcggc
      721 gcgctgctcg acaacatcag cctctacgag gaggcgcagg cggcgatccg ggcccgggac
      781 cacttcctgt cgatcgccgc tcacgagctg cggacacccg cggtgagcat caaggggtac
      841 gcccagttcc tgcttcgcag cctcgaccgc gacacgctct cgaaggaacg acttcgccat
      901 gccctcgaga cagtcgatgc ctcggttctg cgcctctcga cattgaccga cgacctgctc
      961 tcggtgtcgc ggcgcaatat cgaccatctc ccctggcgcc gcgaggtcat caacgtcggt
     1021 cattacctgg tcgacttctt ctcggcctcg ggtccggcgg cgagcaaggg ctacgacgtc
     1081 aacctcgacg tcggtcatgc cgacgtcgag gtgaacgttg acaccgagcg actccatcag
     1141 gtgctgacga tcctgctcgg aaacgccgtc aagttctccc cggaccacga tgagatctcc
     1201 gtcttcgccg aatcggacgc caacggcgtg acgatcacga tcagcgatct cgggatcgga
     1261 ctcctgcccg gggaggaaga caagatcttc gagccattcg gccgcaccga cgaggcgatc
     1321 aaggacaagc tctcgggtct cggcatcggc ctcttcattg cccggtcgat cgtcgaacgg
     1381 catcagggcc ggatctgggc cgagagccag gggcggaatc tgggtgcgag cttcaacgtc
     1441 tggctcccgg cattccgcag gcccgcggtc gatcaccccg agccgccgcc agcatagacc
     1501 tccggtctcg agacccgaga cctttgccac gcgtgctgcc gatcgggcga agggggccgc
     1561 gtttccacgg tccatgagtg tgatttcgag cgtgatagga ggccgcctcg acacattgtc
     1621 gataggcggc ctcctatcac gcgcattcgt gattcgacga tccccgggga gtgacgttgt
     1681 ctgacgaccg agtggcaacc gcacggatgg gtggtcgtcg cgccaagagg acttcacgct
     1741 tcagacgccg cgtggcacgg tcgcgctccg ctcaatgcct ccggaaaaga tgggccttct
     1801 tctcccacct acccatagcc aatgacagtc gatacgatat caccaccaaa cgtacaggaa
     1861 ctatcaaaaa taaagaaact tgtcggaatc cttgacacga actgcacgat cccggtaaag
     1921 tcgtgctcac agtcaaatag acctcatcca gaggggcaga gggaccggcc cgatgaagcc
     1981 ccagcaaccc ttcggcagca aatgccggga aggtgctaac tccggcaggc atacctggaa
     2041 gatgagggga cgttcgcagc ggtctaggcc gtcctgcgtg cgagagaccc cccatcaaca
     2101 tgatgagggg ttttgttttg gataccacat cgtctcacct cttgaacaca gctgctgctt
     2161 tcaatagggc tcccgcgttt cccggtgcgc gctgccgcgc cttgtgtccc cctccgagca
     2221 tctgtcagct attccatcgt gactcagtgc ttgtatcgtc agaaagtgag catacgcatg
     2281 tctcgattcc gtcccacctc ctcgctgtca tctcgccgat ttagtcgccg gc
//
LOCUS       DASTMD010000647         3736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2918097, whole genome shotgun sequence.
ACCESSION   DASTMD010000647 DASTMD010000000
VERSION     DASTMD010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2918097"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFQ54_09520"
     CDS             complement(<1..195)
                     /locus_tag="VFQ54_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165272.1"
                     /translation="MTTTGRNATIARRTGETEIELTLDIDGSGIVRADTGVLFLDHML
                     DAFGRHGRLDLTVRATCDAAM"
     gene            complement(269..1387)
                     /gene="hisC"
                     /locus_tag="VFQ54_09525"
     CDS             complement(269..1387)
                     /gene="hisC"
                     /locus_tag="VFQ54_09525"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEU0165273.1"
                     /translation="MSAQTETPLIASLVRTGVEPVVGYRPGASELTAERSARLDWNES
                     PFGLSPKAQAVLTSFTLGNRYPDFRQTALREALGAYVGVHADRIVPGSGLDDVFETLA
                     LLLIEPGDEVIISEPTFGVYRSLFSMHGAKIVNVPLTPAPDFRLDVDGILAAVTPKTK
                     LVMLCQPNNPTGNLMPKEDIERIVANVPAYVGIDEAYAEFSGANHLDLANSHENVVIF
                     RTLSKFAGLAGFRVGYGVFPESAVPYLNRVSPPFFNITTLSAQIAIASLDDLDHLNAN
                     LQVLLAEREQLAANLAEIDGVEVYPSATNFILASLPVESSEGLLKDLAARNVYVRRFP
                     NPDHHLGHCLRISIGSPEDNRMFLETFTELLDHHRAGS"
     gene            complement(1430..2323)
                     /gene="hisG"
                     /locus_tag="VFQ54_09530"
     CDS             complement(1430..2323)
                     /gene="hisG"
                     /locus_tag="VFQ54_09530"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEU0165274.1"
                     /translation="MTTSRSIINGGDRLKLAIQRSGRLTDSSLELLRHIGLSFESYGQ
                     RLFSKARNYPLSILYARDDDIPDYVDLGTVDFGIVGRNLVHEKGADVAELLALGYGYC
                     SLVVAVPNDSEYTDPEQLKHARIATSYPESARRFFAGIGGDPDIIEISGSVEVAPALG
                     VADAIVELTATGSSLQLNDLKPIHTILESEAVLIANKASMDDPVRRVQIDRLLMRIEA
                     VRAATRYKYVMMNAPESALEEIREILPGQKSPTVVPLADPGWVAVHSAIEEEVFWEKI
                     EALRAAGASAIIVSAIDKFLL"
     gene            2665..>3736
                     /locus_tag="VFQ54_09535"
     CDS             2665..>3736
                     /locus_tag="VFQ54_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEU0165275.1"
                     /translation="MLAKVFSCAVVGLDGVLVEVEVDTGAGQPGIVVVGLPDAAVQES
                     KERVRAAIRNSNGRFPFGKVTVNLAPADLKKAGPTYDLPIALGILLSSGQVFGDLGDA
                     LVVGELSLDGILRHTPGIISMVSLAAEKGLRRAFVPATDAKEAALIAGVEVYGVRTLT
                     DLVNHLTGEVPMTPTTAEIGDATADAWISATDFADVKGQEHVKRGLEVAAAGAHNLVM
                     SGPPGSGKTLLARALPSILPAMSLEEALEVTRIYSVRWLLPPEMPLVRERPFRSPHHT
                     TSHAGLVGGGTWPRPGEISLAHRGVLFLDELPEFQPQVLDSLRQPLEAGEAVIARANS
                     RVTYPARFQLIAAMNPCRCGMAG"
BASE COUNT          659 a         1104 c         1308 g          665 t
ORIGIN      
        1 cattgctgcg tcacaggtcg cgcggaccgt gagatcgagg cgcccgtggc gtccgaaggc
       61 gtcgagcatg tgatcgagga agagcacgcc ggtatcggcg cgtacgattc cagacccgtc
      121 gatgtcgagg gtcagttcga tctcggtttc acctgtgcgg cgggcgatgg tggcgttgcg
      181 accggtagtg gtcatggatg tcgctcctga tggcgttcgg aaggtcggga cgatcaaccg
      241 ctggctgccg ggagctgaat cgctgcgatc agctcccggc gcgatgatgg tccagcagct
      301 cggtgaaggt ctcgaggaac atgcgattgt cctccggcga gccgatgctg atccgcaggc
      361 aatgcccgag gtggtgatcc ggattcggga agcggcggac atagacgttg cgcgcggcaa
      421 ggtccttcag caacccttcg ctgctctcga cggggagact cgcgagaatg aagttcgtcg
      481 ccgacgggta gacctcgacg ccgtcgatct cggcgaggtt cgcggccagt tgctcgcgct
      541 cggcgagcag gacctggaga ttcgcgttga ggtggtcgag gtcgtcgagc gaagcgatcg
      601 cgatctgcgc cgagagcgtc gtgatattga agaacggcgg cgagacccga ttcaggtagg
      661 ggaccgccga ctccgggaag acgccgtaac cgacgcggaa tccggcgaga ccggcgaact
      721 tgctcagggt ccggaagatg accacattct cgtgggaatt cgcgaggtcg agatgattcg
      781 cgccgctgaa ctcggcgtag gcctcgtcga tcccgacgta ggcgggcacg ttcgcgacga
      841 tgcgctcgat gtcctccttc ggcatcagat tgccggtcgg gttgttcggc tggcagagca
      901 tcacgagctt cgtcttcggc gtgaccgcgg ccaggatgcc gtcgacatcg aggcggaagt
      961 ccggcgcagg cgtcagcgga acgttgacga tcttcgcgcc gtgcatcgag aagaggctgc
     1021 gatagacgcc gaatgtgggc tcgctgatga tcacctcgtc gcccggctcg atcaggagca
     1081 gggcgagcgt ctcgaagacg tcatcgagtc cggagcccgg aacgatgcga tcggcgtgga
     1141 cgccgacgta ggctcccagc gcctcgcgaa gcgcggtctg gcggaagtcc gggtagcgat
     1201 tgccgagcgt gaatgaagtg aggactgcct gcgctttcgg cgagagtccg aacggggatt
     1261 cattccagtc gaggcgcgcc gagcgctccg ccgtcagttc cgaggcgccg gggcgatagc
     1321 cgacgaccgg ttcgacgccg gtgcggacga gagatgcgat caacggagtc tcggtttgcg
     1381 cgctcatgga atcaccacat tcaggtggcc gggatgaccc ggcgaacttc tagagaagga
     1441 acttgtcgat cgcggaaacg atgatggcgc tggcgccggc ggcgcgaaga gcttcgatct
     1501 tctcccagaa gacctcctct tcgatcgccg aatgaacggc gacccagccc ggatcggcga
     1561 gcggaaccac cgtcggcgac ttctggcccg ggaggatctc gcgaatctcc tcgagcgcgg
     1621 actccggcgc gttcatcatc acgtacttgt agcgggtcgc cgcgcggacc gcttcgatcc
     1681 gcatcaggag tcggtcgatc tggacccgtc ggacggggtc gtccattgac gccttgttgg
     1741 cgatgaggac cgcctcggac tcgagaatcg tgtggatcgg cttgagatcg ttcagctgga
     1801 gcgaggagcc ggtcgccgtc agctcgacga tcgcgtcggc gacaccgagg gccggcgcga
     1861 cttcgaccga accggaaatc tcgatgatgt ccgggtcgcc cccgatgccc gcgaagaagc
     1921 ggcgcgccga ttccggatac gaggtggcga tgcgggcgtg cttcaactgc tcggggtcgg
     1981 tgtattcgga gtcgttcggc accgcgacga cgagcgagca atacccatat cccagcgcaa
     2041 gcaactcggc gacgtcggcg cccttctcgt gaacgaggtt gcgacccacg atgccgaagt
     2101 ctacggtgcc aagatcgaca taatcaggga tatcgtcatc gcgcgcataa agaatggaaa
     2161 gagggtagtt gcgcgccttc gagaagaggc gttgcccata gctctcgaag ctcaagccga
     2221 tatggcggag cagttcgagt gagctgtcgg tgagacgccc ggatcgttgg atggccagct
     2281 tgagacgatc accgccattg atgatcgatc gcgacgttgt cattgcgacg ggtttctcgc
     2341 ttgaaacgat ggccatctgc caaagcctcg taggtgatgg atggggtcaa aggaaaggtt
     2401 gcgcgaaccg gtgtcgccgg caagggctgg cccgggcagc gtcgttcttg taccacgatc
     2461 gtcgaacttt ccacaagttc cggtcatccg gatcgctctc cgtcctgctg gactcgccga
     2521 cgctcgttgc aattggcgat agggacgaag cccgactgat ggtagagttc atatgacggg
     2581 tccttacgaa aaacagacgt gacgtatcgc gcaagaagcg gtgaccgccg gatcgtgctg
     2641 gcatcgcaag gggtgtgcag agcggtgctg gcgaaggttt tcagttgcgc ggtcgtcggg
     2701 ctggacggcg tgttggttga agtcgaggtc gataccggcg ccgggcagcc agggatcgtc
     2761 gtcgtcgggc ttcctgatgc cgccgtgcag gagagcaagg agcgggtgcg cgccgcgatc
     2821 cggaactcga acggcaggtt tccattcggg aaggtcacgg tgaacctcgc gccggctgat
     2881 ctaaagaagg ctggcccgac ctacgacctc ccgatcgcgc tgggaatcct gctcagttcc
     2941 ggccaggtct tcggcgacct cggcgatgca ctggtcgtcg gtgagctctc gctggacggc
     3001 atcctccgtc acacaccggg catcatttcg atggtctcgc tcgctgccga gaagggattg
     3061 cgccgcgcat tcgttccggc gacggacgcg aaggaggccg ccctgattgc gggtgtcgag
     3121 gtctacggcg tccgcaccct gaccgacctc gtgaaccatc tcactggcga ggtcccgatg
     3181 acgccgacga cggccgagat cggcgacgcg acggcggatg cctggatcag cgcgaccgac
     3241 ttcgccgatg tgaagggaca ggagcacgtc aagcgggggc tcgaagtcgc cgccgcgggc
     3301 gctcacaatc tggtcatgag tggtcctccc ggttcgggca agaccttgct cgcgcgggcg
     3361 ttgccgtcga tcctgccggc gatgagcctg gaggaggcgc tcgaagtgac gcggatctac
     3421 agcgtccgct ggctgttgcc gccggagatg ccgctcgtgc gggagcggcc gttccgctcg
     3481 ccgcatcaca cgacgagtca cgccggtctg gtgggcggcg ggacctggcc gcgaccgggc
     3541 gagatcagcc tcgcgcatcg cggcgtgctc ttcctcgatg aactgccgga gttccagccg
     3601 caggtgctgg attcgctgcg ccagccgctg gaagcgggcg aggcggtcat cgcgcgcgcc
     3661 aacagccgcg tcacctatcc cgcgcgtttc cagctgatcg ccgcgatgaa tccatgccgg
     3721 tgcggcatgg cgggcg
//
LOCUS       DASTMD010000648         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_864742, whole genome shotgun sequence.
ACCESSION   DASTMD010000648 DASTMD010000000
VERSION     DASTMD010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864742"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VFQ54_09540"
     CDS             <1..1130
                     /locus_tag="VFQ54_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0165276.1"
                     /translation="LPSEDAESVDESRRRSRFAYRDAVTAEERGLPPSDDSALLALEQ
                     ETAAEIARAAPNQLTLDFSAVPDGSFMPELAGLTPLEAGSSLELARTWFRRELELGRR
                     PRNTIDSYSYDLQVFEEMTGAKPLNRIDRRDVARFLGEAHSRTTRKRRLTSLRQFFTY
                     LIKTSRVLKIDPTEGYFPHPIQLRSPLPLFTEEQGAMLRAAERDEEWSATAVWLMMRL
                     GLTRSELLAIRRDHIDRASEAVPVVYIYYADLAKQSKERRLAADEEFATIYDRYLEDR
                     RPEDVLFPVGPPAINGMVERVRRAAGITKEVTPQTLRHTFAVEQARQGADATALLQLL
                     GLADDARNRASVGRYIRLAEPPLAPHHEPDGDAPDPDPAES"
     gene            complement(1082..2200)
                     /locus_tag="VFQ54_09545"
     CDS             complement(1082..2200)
                     /locus_tag="VFQ54_09545"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEU0165277.1"
                     /translation="MPDISIVVPRLDEKPWGGRTLETYGFDLPEGTAIGEALVTAGDA
                     AIATGLGADGTLGDIVTGDPAGRLGARATKAVGGREIFPLLVKLIDAAQNLSIQVHPN
                     DEQAAPLDRLGKTEAWHVLAAEPGSRLYVGLRPGVSYEDFAAAARKLDGSSASLLRAL
                     EAKPGTTILLPAGTIHALGAGVMVYEIQQPSDVTFRLDDWGRVDANGNPREMHLEQGF
                     AVGLPDLRPDFIEPIEIAGGRLLVATRYFALERFEIAAGSTREQSGSGSPQVVTVLSG
                     ELTVANATETETLAAGHSVVIWPDTDGSVALGARTDVVALRSWVPDLAADIVAAARAA
                     GASDAAIAAIGSPLADVANVLAGQLSAGSGSGASPSGS"
     gene            2392..>2797
                     /locus_tag="VFQ54_09550"
     CDS             2392..>2797
                     /locus_tag="VFQ54_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU0165278.1"
                     /translation="MSATRSHNGSTPDKNAIRQSIPGRSGNFWAVIPAGGSGTRLWPL
                     SRAARPKFLLPLLGERSLLQQTVDRLLDLTDHEHIMIICGPTHAAEIIRQLPELPTGN
                     VIMEPFQSGTLPALALAAGLIQRQDPDAIMGSF"
BASE COUNT          497 a          998 c          879 g          423 t
ORIGIN      
        1 cccttccgtc cgaagatgcg gaatccgtcg acgaatcccg gcgccggtcg cgattcgcct
       61 atcgcgacgc ggtcacggcg gaagagcgtg gcctgccgcc aagcgatgat tcggcgctgc
      121 tcgcactcga acaggagacg gcggcagaaa tcgcgcgggc cgcgcccaat cagctcacac
      181 tcgacttcag tgccgtcccg gacggctcgt tcatgccgga actggccggt ctgacgccac
      241 tcgaggcggg ctcgagtctc gagctcgccc ggacctggtt ccggcgggaa ctggaactgg
      301 gacggcggcc acgcaacacg atcgactcct acagctacga cctccaggtc ttcgaggaga
      361 tgactggcgc caagccactc aacaggatcg accgccgcga tgtcgcacga ttcctcggtg
      421 aagcgcacag ccgcacgacc cgcaagcgcc gcctgacctc gctccgccag ttcttcacct
      481 acctgatcaa gacgtcacgc gtactgaaga tcgatcccac cgaaggctat tttccgcacc
      541 cgatccagct tcggtcgccg cttcccctct tcaccgagga gcaaggggca atgctccgcg
      601 ctgccgaacg cgacgaggag tggagtgcta ccgcggtctg gctgatgatg cgcctcggcc
      661 tgacgcggtc ggaactgctc gcgatcaggc gcgaccatat cgatcgcgcc tcggaggccg
      721 ttccggtcgt ctacatctac tacgccgacc tggcgaagca atcgaaggaa cgccgtctcg
      781 ccgccgacga ggagttcgcg acgatctacg atcgctacct cgaggaccgt cgtcccgagg
      841 atgtcctctt cccggtcggg cccccagcga tcaacgggat ggtcgagcgc gtgcgacgag
      901 cggcgggcat caccaaggag gtgacgccgc agacgttgcg ccacaccttc gcggtcgaac
      961 aggcgcgcca gggcgcggat gcgacggcgc tcctccagct gctcggactg gccgacgacg
     1021 cccgcaatcg ggcgagcgtc gggcgataca tcaggctcgc ggagccgccc ctggctccgc
     1081 atcacgaacc ggacggcgat gcgccggatc ccgatcccgc ggagagctga ccggccagga
     1141 cattcgcgac gtcagcgagc gggctcccga tcgcggcaat cgccgcatcc gatgcgccgg
     1201 cagctcgtgc cgccgcgacg atgtcggccg cgagatccgg cacccacgac cgcaaggcga
     1261 cgacgtcggt tcgcgcgccg agggcgaccg acccgtccgt atcgggccag atcacaacgg
     1321 agtgaccggc ggcgagtgtc tccgtctccg tcgcgttcgc cacggtcagc tcgccggaca
     1381 ggaccgtcac gacttgcggc gaacccgacc ccgactgctc acgggtagaa cccgcggcga
     1441 tctcgaatcg ctcgagggcg aaatatcgcg tcgcgacgag tagtcgcccg cccgcgattt
     1501 cgatcggctc gatgaaatcg ggacgcagat cgggcaatcc gaccgcgaat ccctgctcca
     1561 gatgcatctc gcgaggattc ccgttcgcgt cgacacggcc ccagtcgtcg aggcggaagg
     1621 tcacgtcaga cggctgctgg atctcataga ccatgacccc ggccccgagg gcgtggatcg
     1681 tcccggccgg caggagaatc gtcgtccccg gcttcgcctc gagcgcccgc aacagggacg
     1741 cggacgatcc atcgagcttg cgcgccgccg cggcgaaatc ctcgtacgag acgcctggcc
     1801 gcagtccgac gtagagccgg gagccgggtt ccgccgcgag aacgtgccac gcttcggtct
     1861 tgccgagccg gtcgagcggc gccgcctgct cgtcgttcgg atgcacctgg atcgagaggt
     1921 tctgggcggc gtcgatcagc ttcacgagca gcggaaagat ctcccgaccg ccgaccgcct
     1981 tcgtcgcgcg cgcgcccagt cgtccagccg ggtcaccagt gacgatgtcg ccgagcgtcc
     2041 catccgcccc gagacccgtc gcgatcgcgg cgtcaccagc cgtgacgagc gcctcaccga
     2101 ttgccgtccc ttccggcagg tcgaacccgt atgtttccag cgttcgtcca ccccacggct
     2161 tttcgtccaa tcgcggcacg acgatcgaga tatccggcat tgaacgctcc ttgtatgctg
     2221 atgacctggc aagggtcgcg aatcgagacg cgcgatccac gtacactgga cagaatgata
     2281 tggcacgaag gatcgcggca ggaccgatcg cggacgcacc ccggcaatga cgaatcgatc
     2341 catcgaccgc ccgatccacc ggcaccggct atcagcggaa ggactccagg catgtcggcc
     2401 acgcgttcac acaacgggtc cacgccagac aagaacgcga ttcgccaatc gattcccggt
     2461 cgcagcggga atttctgggc ggtgatcccg gcgggtggtt ccgggacgcg cctctggccg
     2521 ctgagccgcg ccgcgcgacc gaaattcctc ctgcccctgc tcggagagcg atcgctgctg
     2581 cagcaaacgg tcgaccgcct gctcgatctg accgaccatg agcacatcat gatcatctgc
     2641 gggcccaccc acgccgccga gatcatccgc cagttgccgg agctcccgac cggcaacgtg
     2701 atcatggagc cgttccagag tggcacgctt ccggccctcg cgctcgccgc cggtctgatc
     2761 cagcggcagg acccggacgc gatcatggga agcttcg
//
LOCUS       DASTMD010000649         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_864789, whole genome shotgun sequence.
ACCESSION   DASTMD010000649 DASTMD010000000
VERSION     DASTMD010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864789"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFQ54_09555"
     CDS             complement(<1..556)
                     /locus_tag="VFQ54_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0165279.1"
                     /translation="MTATGIPRPVDARYVPEVVAPGIIRIDLGFQGTAGVIASYLLVG
                     DDELTLIETGPTSTRPNLELGIREAGYDIADVTHLIPTHIHLDHAGAAGGLMQDHQRM
                     RMHLHPVGAPHLIDPARLVSSASRIYGDRMDALWGEVVGAPAERVETVEDGDVVNVAG
                     RSLRAIYTPGHAGNHIAWFDEASRT"
     gene            814..1128
                     /locus_tag="VFQ54_09560"
     CDS             814..1128
                     /locus_tag="VFQ54_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0165280.1"
                     /translation="MDSQRNLFSVAEAARQLERSTEQVRRYLREGRLEGRRMGGQWFI
                     ERSALDTFHQAARARHDFIARLKPASELRVLDNVIGIGEGPGSDISSGKRLYRAVGSS
                     RR"
     gene            1152..1595
                     /locus_tag="VFQ54_09565"
     CDS             1152..1595
                     /locus_tag="VFQ54_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0165281.1"
                     /translation="MDYAEDSASEDGTDAALPDIPVAFVDSSAIVAIVDRNDASHAAA
                     SEAYQALVDGGYRLFTTNHVVAEALDLLTVGVGAEIARQFLRDHRLPIYHVEEQDEER
                     ARSLIANSRSARGLSYADATSLVVMERFGVADAFAVDPSFLGDSD"
     gene            1655..>2010
                     /locus_tag="VFQ54_09570"
     CDS             1655..>2010
                     /locus_tag="VFQ54_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165282.1"
                     /translation="MSASDPTTQRICPECGSANSKLSLFCAECGASLNGGADAAPSPA
                     AAARFDVEEDPQQTQPLTDGIPDREPPSPWALNGSSSARWSPDSTETAYTSPINATSV
                     SAPPIATRFDVDSTAEA"
BASE COUNT          368 a          660 c          629 g          353 t
ORIGIN      
        1 gcgtgcggct cgcctcgtcg aaccaggcga tgtggtttcc cgcatggcct ggtgtgtaga
       61 tcgcgcggag cgagcgaccg gcgacgttga caacgtcgcc gtcctcgacg gtctcgacgc
      121 gttccgccgg cgcaccgacg acttcccccc agagcgcatc catgcggtcg ccgtagatgc
      181 gactggcgct gctcacgagg cgcgcgggat cgatcagatg gggcgccccc accgggtgca
      241 gatgcatccg catccgttgg tggtcctgca tcagcccgcc cgccgctccg gcatgatcga
      301 ggtggatatg ggtcgggatc agatgcgtca catcggcgat atcgtatccg gcttcccgaa
      361 tgccaagctc gagattgggc cgcgtgctgg tcgggccggt ctcgatcaac gtcaactcgt
      421 cgtcgcccac gaggagatag gaagcgatga cgccggcggt tccctggaat ccgagatcga
      481 tccggatgat gccgggtgca accacctccg gcacatacct cgcgtcgacg ggtcgcggaa
      541 ttcctgtggc agtcacgact ggattcgtcc tttcatcgtg cgccaaccca cctggtgcga
      601 tgacttcgag tcccacccga gggttgcgac acttgcccta atcggtcata aatcactacc
      661 atgtagttgc atacgacgca acatcctggt cgacttcctc attccacgag tattcagtcc
      721 gcagaagctc acgccgggag tatcgtcgtg acgagtggcg acgacgccct tgacgcagca
      781 tcacggaagg cccggatccg ggcaagaggc agcatggata gtcagcggaa tctcttcagc
      841 gtcgccgagg cggccagaca gctcgaacga tcgaccgaac aggttcgccg ctacctccgt
      901 gaaggtcggc tcgagggccg gcgaatgggc ggtcagtggt tcatcgagcg atccgcgctc
      961 gataccttcc atcaggcagc gcgggcccgt cacgacttca tcgcgcgcct gaaaccggcg
     1021 agcgaacttc gcgtactcga taatgtgatc ggaatcggcg aaggaccagg cagcgacatc
     1081 agcagcggga agcgcctcta tcgtgccgtc ggcagcagtc gccgctagtc tcgtcgaatg
     1141 gggaagcacg aatggattac gccgaagatt cagcaagcga agacggaaca gatgccgccc
     1201 ttccggacat tcccgtcgcc ttcgtggatt cgtccgcgat cgtcgccatt gtcgatcgga
     1261 acgatgcctc gcatgcggct gcatccgagg cctatcaggc gctcgtagac ggaggatatc
     1321 gcctcttcac gacgaaccac gtcgtcgcgg aggcgctcga tctcctgaca gtcggtgtcg
     1381 gggccgagat tgcgcggcag ttcctgcgcg atcaccggct gccgatctac cacgtcgagg
     1441 agcaggacga ggagcgcgcg cgatcgctga tcgccaattc ccggagtgcg cgcggtctct
     1501 cctacgcgga cgcgacgagt ctcgtcgtga tggagcgctt cggtgtcgcc gatgcgttcg
     1561 cggtcgatcc cagcttcctg ggtgactcgg actgacctac gccgcgggag ccatcgatcc
     1621 cgcaggcaga cagcgaaaag agccagctat cctcatgagt gcctcggatc ccaccacgca
     1681 acgtatctgc cccgagtgcg gtagtgccaa cagcaagctc agcctcttct gcgcggaatg
     1741 cggcgcgtcc ctcaacggcg gcgcagacgc cgccccatct ccggcggcag ccgctcgctt
     1801 cgatgtcgag gaagatcccc agcagacgca accgctgacg gacggcattc cagatcggga
     1861 gccgccatcg ccatgggcgc tgaacggctc gtcctcagcc cgctggtcgc ctgattcgac
     1921 cgagaccgca tatacgtcgc cgatcaacgc gacgtcggtt tcggccccgc cgatcgccac
     1981 gcggttcgac gtcgattcca cggcggaggc
//
LOCUS       DASTMD010000650         2538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_953055, whole genome shotgun sequence.
ACCESSION   DASTMD010000650 DASTMD010000000
VERSION     DASTMD010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953055"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFQ54_09575"
     CDS             complement(<1..411)
                     /locus_tag="VFQ54_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165283.1"
                     /translation="MGRSRYSSRRERPSPQAEITAQLGLLKDLVNDPVALRATAVEFI
                     QHASSLEVTRAAIVTLQGMNDPANRDVLHRRYEVSGEKNDSGGFIRAAIVRALQPIVS
                     VDDRPLLMRALNTYQMQGMYEISAELRTAALIAMN"
     gene            complement(699..1910)
                     /locus_tag="VFQ54_09580"
     CDS             complement(699..1910)
                     /locus_tag="VFQ54_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165284.1"
                     /translation="MNDATRFSRLRHLFRPSRGNDATAMEFDRQLDALVTGMPAPAPD
                     AASLDTDRARVDVLTATFLQAASLRTAQEVAMTASGRTKARIWEDIMSATASPSLSPA
                     ARPFAGPPPSSNSTPIRNVGRSIRTWTARFSPVLNVAMVALMILTLVLGAFAVTNRGG
                     WHPNGGPSGNNLAMLPATVEGTPVAQVDLPTAADCTVKPLTVDEVIDRIKHPYPFYPE
                     SPATPVPSQAVYTQPKSGPLSTKDLAEISDVQRQFDACILKGSPFQLWALIIQGSRFW
                     ADIVKAYPPLTSEATVRSDLEELLESGNDPNVVNKTRLLIYSPKSLNPYGLPLVNPDP
                     AASIYTEEGPTNVIAPTAEVATLYYDPNNPTAAPTNTPFDQMVQPWFYRWDYSTDSWK
                     IDDIGILEARG"
     gene            complement(1907..>2538)
                     /locus_tag="VFQ54_09585"
     CDS             complement(1907..>2538)
                     /locus_tag="VFQ54_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0165285.1"
                     /translation="TATATATPDIDLHGEFAIVVATLADPRAFAPLYERYFPLIHGYC
                     LRRLGHPENAADATSQIFINAIQALPKFRPDLQRPGSSFRSWLFAIAHNVVVDHYRRT
                     RPHLSLDAPATRGDDLDLDSRDVPDTDPTPEEYAVATESRDYLASLLNQLPERQAAVA
                     ELRLAGFTNAEVAEALRISYPAVRSAQYRAFLALRELLESPTASTGDRT"
BASE COUNT          462 a          703 c          885 g          488 t
ORIGIN      
        1 gttcatcgcg atcaacgcgg cggtgcggag ctcggcgctg atctcgtaca tcccctgcat
       61 ctggtaggtg ttcagcgccc gcatcaggag cggacggtcg tcgacgctga ctatcggttg
      121 cagggcgcgg acgatcgcgg cgcgaatgaa gcctccagaa tcattcttct cgccggagac
      181 ctcgtagcga cggtggagaa cgtcacggtt cgccggatcg ttcattccct gcaacgtcac
      241 gatcgccgct ctcgtgacct cgagggacga cgcgtgctgg atgaactcga cggcggtggc
      301 gcggagggcg accggatcgt tgacgagatc cttcagcagc ccgagctggg ccgtgatctc
      361 cgcctgcggc gagggccgct cccgtcgact cgaataccgc gatcgtccca cgtgcgctct
      421 ccctggtcac gaatcggcca taggagagag cctagcacga gagcgcgcgc ggtacccaaa
      481 ggcgggacac ggggggactt ctatgtcccc ccttttgccg tcacagggaa gtgcgcatcg
      541 ttcggaggtt ggggttgcgg cggggtataa aaccccgccc taccaacccc ggtcggttgc
      601 cgacatctcg gtcgacgtgg attgcgttct ggtgcgagcg cttctcgagc aatcgagacg
      661 actgtgaggg catggggtca gatggaaacc aactgccgtt accctcgtgc ctcaagaatg
      721 ccaatatcgt cgatcttcca ggagtcggtc gagtagtccc atcgatagaa ccatggctga
      781 accatttgat cgaatggcgt gttcgttggc gccgctgtcg gattattcgg atcgtaatag
      841 agtgtcgcca cctcggcagt cggcgcgatc acatttgttg ggccctcctc agtgtagatc
      901 gatgcggctg ggtcgggatt gacgaggggc aatccatagg ggttgagcga tttcgggctg
      961 tagatgagga gccgggtctt gtttaccacg ttggggtcat tccccgactc cagcaattct
     1021 tcgagatcgc tacgaactgt agcctcggat gtgagtggag gataagcctt cacgatgtct
     1081 gcccagaacc gggagccttg aatgatcaag gcccagagtt gaaacggact tcccttcaat
     1141 atgcacgcgt cgaactgtcg ttgcacgtct gaaatctctg cgaggtcctt ggtactcaat
     1201 gggccactct tcggttgggt atagacggcc tggcttggaa cgggcgtagc aggtgattcc
     1261 ggataaaacg gatagggatg tttgatccgg tcgatcacct cgtcgacggt caggggcttg
     1321 accgtgcaat cggcggcggt cgggagatcg acctgggcga caggtgttcc ttccactgtg
     1381 gcgggcaaca tggcgaggtt attaccgctc ggtccgccgt tcggatgcca cccaccccga
     1441 ttcgtgaccg cgaacgcgcc gagtaccagc gtcaggatca tgagcgcgac catcgccacg
     1501 ttgagaaccg gtgagaagcg tgccgtccag gtgcggatcg agcgtccaac gttgcggatg
     1561 ggcgtggaat tcgatgacgg tgggggacct gcgaagggtc tcgcggctgg tgacaacgac
     1621 ggtgaggcgg tcgcagacat gatgtcctcc cagatgcgtg ctttcgtgcg cccgctcgct
     1681 gtcatcgcga cttcctgagc ggttcgaagt gacgccgcct gcaggaaggt cgcggtgagc
     1741 acgtcgacac gggcgcggtc ggtatcgaga ctcgctgcat cgggtgcggg agccggcatt
     1801 cccgtcacca gggcgtcgag ctgacgatcg aactccatcg cggtggcgtc gttgccacga
     1861 gatgggcgga agagatggcg aagtctggag aaacgcgtcg cgtcgttcat gtgcggtctc
     1921 cggtggaagc ggtcggtgat tcgaggagtt cgcgcagggc gagaaaggcg cggtattgcg
     1981 ccgagcggac ggcgggatag gagatccgga gcgcctcggc gacctccgcg ttcgtgaagc
     2041 ccgcgaggcg cagttcggcg acggccgcct gccgctccgg aagctggttc aggagcgacg
     2101 cgagataatc gcgcgattcg gttgcgaccg cgtattcctc cggtgtggga tcggtgtccg
     2161 gaacatcacg tgaatcgaga tcgagatcgt cgccccgggt ggcgggagcg tccagcgaga
     2221 gatgcggacg cgtgcggcga tagtgatcga cgacgacgtt gtgggcgatc gcgaagagcc
     2281 agcttcgaaa ggacgatccc gggcgctgaa gatcgggccg gaacttcggc agcgcctgga
     2341 tcgcgttgat gaagatctgg gacgtcgcgt cggcagcatt ctccgggtgg ccgagacgcc
     2401 gcaggcagta gccgtggatc aagggaaagt agcgttcgta gagcggcgcg aaggcacggg
     2461 gatcggcgag cgtggcgacg acgatcgcga actcaccgtg gagatcgatg tctggtgtcg
     2521 ccgtggcggt cgcggtcg
//
LOCUS       DASTMD010000651         7193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2651279, whole genome shotgun sequence.
ACCESSION   DASTMD010000651 DASTMD010000000
VERSION     DASTMD010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2651279"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..825)
                     /locus_tag="VFQ54_09590"
     CDS             complement(175..825)
                     /locus_tag="VFQ54_09590"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU0165286.1"
                     /translation="MANAITVGRLVLLFVVVWLIHVGNVGVIETSMILIILVFGSDGV
                     DGWVARKRNSTSTFGAVFDIAGDRIVENVLWVVFADLNLIPVWIPLMVLTRGFIVDAL
                     RSLHVTEGTTSFGGSKMMRSAFTEWLTAGRFMRAFYGYAKAAGFVFLTGLAGDRRLDT
                     SGTALGTLYGWDWFHFVGWFCVWAAVALTVIRGLPVIYDSLAYIREVDRRDGGPKS"
     gene            complement(946..1644)
                     /locus_tag="VFQ54_09595"
     CDS             complement(946..1644)
                     /locus_tag="VFQ54_09595"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU0165287.1"
                     /translation="MVNVYAHRGASAEQPENTIAAFRRALEIGMYGIELDVYLSKDGV
                     AVVIHDSSVNRTTNGSGELSDLDFAEIRALDAGRGESVPTLEDVIALVAGKTHLNIEV
                     KADAAADEVLRLASQASGLSWGISSFDWDVLRYARRRDSGIDLWPLGIGATEDALKVV
                     QEIGANQLNLFDPAIDEDIVAFLAERGIGTWVWTVNDPARAALLAEWGATGICTDNPR
                     VIQDALATASRSGR"
     gene            1768..2133
                     /locus_tag="VFQ54_09600"
     CDS             1768..2133
                     /locus_tag="VFQ54_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU0165288.1"
                     /translation="MISVAARLVEMHPQTLRKYEREGLIAPSRTSGNLRLYSDSDIER
                     LRQVKYLVEQRGLNLAGVQLALELTHQVQDLRGRIVAATDGRKQSPVVAELDAVLESL
                     GFVDEQKLRDTQRRDQTRT"
     gene            2261..4867
                     /gene="clpB"
                     /locus_tag="VFQ54_09605"
     CDS             2261..4867
                     /gene="clpB"
                     /locus_tag="VFQ54_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEU0165289.1"
                     /translation="MATNQQFTEKAREAVLAGQQITEEKKLAQFEPEALLYGLVIQGD
                     GVVPQILQRLGVDPAQLLREVEVLIDQAPKLQYSADPVVSSGLRRALQTAESEARQFG
                     DEFISAEHLFLGILAVERSPGEALLRRYNISKDRTYGALNEIRGNQRVTDPNPEGKYQ
                     ALEKYGRDLTTWAEEGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIAE
                     GLAQRIVRGDVPEGLMDKRIVALDLGALIAGAKFRGEFEERLKAVLKEVESSSGRIIL
                     FIDELHTVVGAGAAEGSMDASNMLKPMLARGELHAIGATTLDEYRKYIEKDAALERRF
                     QPVMVGEPAVEDTISILRGLRERYEVHHKVKILDSALVAAATLSDRYIADRFLPDKAI
                     DLVDEAASKLRMEITSMPVELDEAERRIMQLEIEREALRNEKDEASQQRLAILERDLA
                     NIKEERDALRSKWDQERQAIHAISELRDEMDRTRIEMEQAQRAYDYNKAAELQYGKLK
                     ELDQQIREEEQKLARLQASGKLLKEEVDADDIAEVVSRWTGIPITRLMEGEIQKLVHM
                     EERLHQRVVGQEEAIEAVANAIRRARSGLSDPNRPMGSFIFLGPTGVGKTELARALAE
                     FMFDSDQAMVRIDMSEYMERHSVSRLVGAPPGYVGYEEGGQLSEAVRRRPYSVILFDE
                     IEKAHPDVFNLLLQILDDGRLTDGQGRTVDFRYTVIIMTSNLGSQFILGVNNDYEQMR
                     LSVMEALRAHFRPEFLNRIDETIIFHQLTREQIGDIVQFQLDNVNARLAERRMSVQLT
                     TAARELISTLGYDPLFGARPLKRVIQKHILDPLAMKLLNGEIREGEEIYVDVADGALV
                     FKGALTSAPLAA"
     gene            5012..5146
                     /locus_tag="VFQ54_09610"
     CDS             5012..5146
                     /locus_tag="VFQ54_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165290.1"
                     /translation="MAIVLGIIRAFIMLGFIAVVIALLAGLFMVVFGIASSIGQANRE
                     "
     gene            5336..>7193
                     /locus_tag="VFQ54_09615"
     CDS             5336..>7193
                     /locus_tag="VFQ54_09615"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEU0165291.1"
                     /translation="MPEYAAPTNLQRRSLSIALVMLLALSFFATSMSLGGSARAAQTS
                     TLKDTTAALTPASALAYVDINLDQNSDQWHLSTDLLKRAGLDDVLSEGNVTSTETETA
                     DSFLGGSAALVLTKLPATGDVSLDSLTGEVTGAATDPASLTQSTIPSGFSVIFQSPDL
                     ASVEAQLKETLGDNADSDGSTVKTETYEGVVISSLTPTDPTATGTAYAVVKDSLVVAT
                     VAGDIKPIIDAATGKVATLADDTSYKTLKGDLNSDSLAFGLVNGTALLAQAKKQDPTV
                     YADLDPTQVAQFDGTVGFVLWADQPGFRIDTLTLANPDATAAKAPAPLDGDFASKVPA
                     DSLFFANGTNINQFGILDSLGLVFAQALVGTGEEGIDGTPTAATPVSATEQAAQIYEQ
                     AAALLGFNIKTDFIDQLTGEFGVAVSASDLTSETPNISAIFATDVNDSQKVTDVASKI
                     SFILSSALGSTGLSSRDVKGSSITSIDLSGDTSTPEAGTPDASSDASSPLKIEYGVVD
                     GQLLIGVNDGIDDYVNGPTDSLADSKVYKDTLAQLPQNPTGIVFVNVQEILPLLDEAA
                     SDLAVTATSVTVRDASPDCAKYDTQQEAQDAYDADSFTNFDLDEDLDGQACED"
BASE COUNT         1456 a         2313 c         2192 g         1232 t
ORIGIN      
        1 gctggcgatc gcgagctgat atcgaaactc gtgccgttct tcccgacgga agatcgggag
       61 tctggatttc ttctgctcct cgtcggacaa gttggctccg attacgctgc gtttcacgct
      121 gccacgcgcg ggccaccggc ccgtccgtgc cctcggtgga agatacgctc aggatcagct
      181 ttttggaccg ccgtcgcgcc ggtcaacctc gcggatatag gcgagtgaat cgtagattac
      241 gggaagtcca cggataacgg tcagcgccac cgccgcccaa acacaaaacc agccgacaaa
      301 atgaaaccag tcccaaccat agagtgtccc cagcgccgtt cctgaggtat cgagtcgccg
      361 atcgccggcg agtccggtca ggaaaacgaa ccccgccgcc ttggcgtagc cgtagaaggc
      421 gcgcatgaaa cgaccggcgg tcagccactc ggtgaaggcc gatcgcatca tcttcgatcc
      481 gccgaaggac gtcgttcctt ccgtcacgtg gagcgaccgc agcgcgtcga cgatgaaacc
      541 gcgcgtcagc accatcagcg ggatccagac gggaatcaga ttgagatcgg caaagaccac
      601 ccagagtacg ttctcgacga tccggtcacc ggcgatatcg aaaacggcgc caaacgtcga
      661 cgtcgagttc cgcttccgcg ccacccaccc gtcgacacca tcgctgccga agacgaggat
      721 gatcaggatc atcgaggtct cgatcacccc gacgttaccc acgtggatga gccacacgac
      781 aacgaacagc aggacgaggc gtcctaccgt gatcgcgtta gccatggttc ggcccctggc
      841 agcgtccagg ggaccgggtt gctgatctcg ccacacatcc ctcgcattgg agaatcctgt
      901 gcatcacatt catgaattga ttatcgccca gcggtcagcg ctccctcaac gtcccgatcg
      961 ggaagctgtg gcaagagcgt cctgaatgac gcgtgggttg tccgtgcaga tcccggtcgc
     1021 accccactcc gcgagcagcg ccgcccgcgc cgggtcgttg acggtccaga cccaggtccc
     1081 gatgccccgt tcggcaagaa atgcgacgat atcctcgtcg atcgcgggat caaagagatt
     1141 gagctggttc gcgccgatct cctgaaccac cttcaatgcg tcctcggtgg cgccaatccc
     1201 gagcggccag agatcgatcc ctgagtcacg acgacgcgcg tagcgcagca catcccaatc
     1261 gaacgaggag attccccacg agagacctga agcctggctc gcgagtcgca ggacctcgtc
     1321 ggctgcggca tccgccttca cctcgatgtt gagatgcgtt ttgccggcga cgagcgcgat
     1381 cacgtcctca agcgtgggaa cggattcgcc ccgaccggca tccagtgcgc ggatctcggc
     1441 gaagtcgaga tcggagagct cgccgctgcc attggtcgta cgattgacgc tggagtcgtg
     1501 gatcacgacg gcaacgccat ccttcgagag atagacgtcc agctcgatgc cgtacatgcc
     1561 gatctcgagc gcgcgtcgga acgccgcgat cgtattctcg ggctgttccg ccgaggcgcc
     1621 ccggtgcgcg taaacgttga ccacccttgc tccctccgag ttgaattgca caatcacatt
     1681 gacacggtcc gtatcaagaa gtatcattca cgcaggtcgt acttcggggg tatatgcatg
     1741 gcaccaggat ttcaggaacc gctgtttgtg atcagcgtcg cggctcgtct cgtcgagatg
     1801 cacccgcaga cgctacgtaa atacgaacgc gagggtttga tcgcgccgtc ccgcacgtcg
     1861 ggaaatctgc gcctctactc cgacagcgac atcgaacggc tgcgacaggt gaaatacctt
     1921 gtcgagcagc gcggtctcaa tcttgccggt gttcagctcg cgcttgagtt gacgcatcag
     1981 gtccaggatc tccgcggacg gatcgttgcc gcgacggatg ggcgaaagca gtcgccggtg
     2041 gtcgccgaac tcgatgccgt tctcgaatca ctcggattcg tggatgagca gaagctgcgg
     2101 gacacacagc gccgggacca gacgcggacc tgagcccgat atctccatag ttcacgattg
     2161 ccgccgatcc gtcaaaccag aacgtggttg cggcactatc ggcggcgatt tctcgttcct
     2221 tctttctacc ttcctgctta cgcaaggagc gaattgatac atggcaacca atcaacagtt
     2281 cacggagaaa gcccgggaag cagttctcgc cgggcaacag attaccgagg agaagaagct
     2341 cgcccagttc gagccggaag cgcttctcta cgggctcgtg atccagggcg acggggtcgt
     2401 tccccagatc cttcagcgac tcggcgtcga cccggcgcag ctgctgcgcg aagtcgaggt
     2461 cctgatcgat caggcgccga agctccagta cagcgccgac ccggtcgtca gttcaggtct
     2521 gcgccgcgcg ctgcagacgg cggaatcgga agcacggcag ttcggcgatg aattcatcag
     2581 tgccgagcac ctcttcctgg gaattctcgc agtcgagcga tcgcccggtg aggcgctcct
     2641 gcgccggtac aacatctcga aggaccgcac ctacggggcg ctcaacgaga ttcgtggcaa
     2701 tcagcgcgtg actgacccca accccgaggg caaataccag gccctggaga agtacggtcg
     2761 cgacctgacg acctgggccg aagaaggcaa gctcgatccg gtgatcggac gcgacgagga
     2821 gattcggcgc gtcatccagg tgctctctcg ccggaccaag aacaacccgg tcctgatcgg
     2881 cgagcccggc gtcggcaaga cggcgatcgc cgagggtctc gcccagcgga tcgttcgtgg
     2941 cgacgtcccg gaaggtctga tggacaagcg catcgtcgca ctcgacctcg gcgccctgat
     3001 cgcgggcgcg aagttccgcg gcgagttcga ggagcggttg aaggcggtcc tgaaggaggt
     3061 cgaatcctcc agcggcagga tcatcctctt catcgacgag ttgcacacgg tcgtcggtgc
     3121 gggagccgcc gaggggtcga tggacgcctc gaacatgctg aagccgatgc tagcgcgcgg
     3181 tgagctccat gccatcggcg cgacgacgct cgacgagtac cgcaagtaca tcgagaagga
     3241 cgccgctctc gagcgacgct tccagcccgt gatggtcggc gagccggcgg tcgaggatac
     3301 gatctcgatc ttgcgcggac tccgcgagcg gtatgaagtc catcacaagg tgaagatcct
     3361 cgactcggcg ctcgtcgccg cggcgacgct ctccgatcgc tatattgccg accgtttcct
     3421 gccggacaag gcgatcgatc tcgttgacga ggccgcatcg aagctccgca tggagatcac
     3481 ctcgatgccc gtcgaactcg acgaggccga gcggcgcatc atgcagctcg agatcgagcg
     3541 cgaggcgctt cgcaacgaga aggacgaggc ctcccagcag cgcctcgcca tcctcgagcg
     3601 cgacctggcg aacatcaagg aggagcgcga cgcgcttcgc tcgaagtggg atcaggagcg
     3661 gcaggcgatt cacgccatct ccgaactccg cgacgagatg gaccgcaccc gcatcgagat
     3721 ggagcaggcg cagcgagcct acgattacaa caaggcagcc gagctccagt acgggaagct
     3781 gaaggaactc gatcagcaaa tccgtgagga agagcagaag ctggcgcggc tccaggcttc
     3841 cggcaagctt ctgaaggagg aggtcgacgc cgatgacatc gcggaagtcg tcagtcgctg
     3901 gaccggcatt ccgatcacgc gcctgatgga aggcgagatt cagaagctcg tccatatgga
     3961 ggagcggctg caccagcgag tcgtcgggca ggaggaagcg atcgaggcgg tcgcgaatgc
     4021 gatccgtcgt gcgaggtccg gtctcagcga cccgaatcga ccgatgggaa gcttcatctt
     4081 cctcggcccc accggcgtcg gcaagacgga gctcgcccgg gcactcgccg aattcatgtt
     4141 cgatagcgac caggcgatgg tccgcatcga catgtcggaa tacatggagc ggcactcggt
     4201 cagccggctg gtcggtgcgc ctccgggcta cgtcggatac gaggaaggtg gacagctttc
     4261 cgaggcggtc cgccggcgcc cctacagcgt gatcctcttc gacgaaatcg agaaggctca
     4321 cccggatgtc ttcaatctgc tcctgcagat cctcgacgac ggacgcctga cggacggcca
     4381 ggggcgaacg gtcgacttcc ggtacacggt catcatcatg accagcaatc tgggatcgca
     4441 gttcatcctc ggcgtgaaca acgactacga gcagatgcgg ctgtcggtga tggaggcgct
     4501 tcgcgcgcac ttccggccgg agttcctgaa ccggatcgac gagacgatca tcttccacca
     4561 gttgacgcgg gagcagatcg gcgacatcgt ccagttccag ctcgacaacg tcaacgcgcg
     4621 gctcgccgag cgccggatgt cggtacagtt gacgacagca gcgcgcgagt tgatctcgac
     4681 actgggctac gacccgctct tcggagcccg tccgctcaag cgggtgattc agaagcacat
     4741 cctggatccg ctcgcgatga agctcctcaa cggcgaaatc cgggagggag aggagatcta
     4801 cgtcgacgtg gcagacgggg cgctcgtctt caagggcgcg ctcacctcgg cgccgctcgc
     4861 cgcctagact cccacctcaa gcacgaaagc actatcgaaa ggcccggtcc tcatggatcg
     4921 ggcctttatt tgcttccggt gccaaattgc cgccattgca agcgactata ctcggcagaa
     4981 cggctgtcgg aatcgagcaa caggatgaca agtggccatc gttctcggga tcatcagggc
     5041 gttcatcatg ctgggattca tcgcggtcgt gatcgcgctg ctcgccggcc tattcatggt
     5101 cgtcttcggc atcgcatcga gcattggcca ggcgaatcgg gaatagatta gccaccggag
     5161 gaccgctttc cacgacgcgc ggggaacgac catccgtatc acgacgttca tctctgtgag
     5221 tctctgatcc agggtgggcg cgtcgcatcc ccggtccgat cgcacccgaa aatccgacag
     5281 tcgacgcggt tccacgacaa tctggtactt tcaccaatcc ggaaggagcg cgcacttgcc
     5341 cgaatacgct gcacccacaa acctccaacg acggagtctt tccatcgctc tcgtcatgct
     5401 cctcgcattg agcttcttcg caacgtcgat gtcgctgggc gggagcgccc gcgcggcgca
     5461 gaccagcacc ctcaaggaca cgacggcagc attgacgccg gcatccgcac tcgcgtacgt
     5521 cgacatcaac ctcgatcaga attccgacca gtggcatctc tcgaccgatc tcctgaagcg
     5581 ggccggtctc gacgatgtcc tcagtgaggg caacgtcacc agcaccgaga ccgaaacggc
     5641 cgactccttc ctcggaggca gtgctgcgct cgtcctcacg aagctgccag cgacgggcga
     5701 tgtttcgctc gattcgctga ccggtgaagt caccggtgcc gcgactgatc cggcatcgtt
     5761 gacgcaaagc acgatcccgt ccggcttctc ggtgatcttc cagagtccgg atctggcgag
     5821 cgtcgaggcc cagctcaagg agacgctggg cgacaatgcc gattccgacg ggagcacggt
     5881 caagacggag acctatgagg gcgtcgtgat cagctcgctg actcccaccg atccgacggc
     5941 gacgggtacc gcctatgccg tcgtcaagga ctcgctcgtc gtcgccaccg ttgcgggcga
     6001 catcaagccg atcatcgacg ctgccaccgg caaggtggcc accctggctg acgacacgag
     6061 ctacaagacg ctgaagggcg acctcaacag cgattcgctc gcctttggcc tcgtcaacgg
     6121 tacggcgctt ctcgcccagg cgaagaagca ggatccgacg gtctatgccg atctcgaccc
     6181 gacccaggtc gcgcagttcg acggcacggt cggattcgtc ctgtgggcgg atcagccagg
     6241 gttccgcatc gacacgctga cgctggcgaa cccggacgct accgccgcga aggcgcccgc
     6301 cccgctcgac ggcgactttg cctcgaaggt gccggccgat tccctcttct tcgccaacgg
     6361 cacgaatatc aaccagttcg gcatcctgga ttcgctcggc ctggtcttcg cccaggccct
     6421 ggttggcacg ggcgaggaag gcatcgacgg aacgccgacc gcggccactc cggtctccgc
     6481 gaccgagcag gcggcccaga tctacgagca ggccgcggcg ctgctcggct tcaacatcaa
     6541 gacggacttc atcgatcagc tcacgggcga gttcggagtc gcggtttcgg cgagcgatct
     6601 cacgagcgaa acaccgaaca tcagcgcgat cttcgcaacg gacgtcaatg actcccagaa
     6661 ggtcaccgat gtcgcgtcga agatctcgtt catcctctcc tccgcgctcg ggagcacggg
     6721 tctgagctcc cgcgatgtca aggggagcag catcacctcg atcgatctga gcggtgacac
     6781 gagcacgccc gaagcgggca cgccggacgc ctcgtcggac gcaagcagcc cgctcaagat
     6841 cgagtacggc gtcgtcgacg gtcagctcct gattggcgtc aacgacggta ttgacgacta
     6901 cgtcaatggt ccgaccgact cgctcgctga cagcaaggtc tacaaggaca ccctggcaca
     6961 gcttccgcag aacccgaccg gcatcgtctt cgtgaatgtg caggaaatcc tgccgctcct
     7021 cgacgaagcc gccagcgatc tggcggtcac cgcgacgtcg gtgacggtgc gcgacgccag
     7081 tccggactgc gcgaagtacg acacgcagca ggaggcgcag gacgcctacg acgccgacag
     7141 cttcacgaac ttcgatctcg acgaagatct cgatggccag gcatgcgagg act
//
LOCUS       DASTMD010000652         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_59977, whole genome shotgun sequence.
ACCESSION   DASTMD010000652 DASTMD010000000
VERSION     DASTMD010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59977"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..541
                     /gene="hslV"
                     /locus_tag="VFQ54_09620"
                     /pseudo
     CDS             35..541
                     /gene="hslV"
                     /locus_tag="VFQ54_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
     gene            676..2169
                     /gene="hslU"
                     /locus_tag="VFQ54_09625"
     CDS             676..2169
                     /gene="hslU"
                     /locus_tag="VFQ54_09625"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEU0165292.1"
                     /translation="MAEPVRRTTGKDKSVRIGGGDSETERPADSLTPKEIVAELDRYI
                     IGQDEAKRAVAVALRNRYRRQQLSDDLRQEVQPKNILMIGSTGVGKTEIARRVARIVD
                     APFIKVEATKFTEVGYVGRDVESIVRDLVEIAIAMMHTEQLENVRDEAANAAVERLAD
                     IMSEQMIQQRPIRPHRKNGHEPQEDEQEIALREARSVERRRRREHKRIVDLLNQQAID
                     DETVEIEFDPDMDFEEGGAFEFAPGGNPDEFQETFYDFLDSMMPRRRMRRRVSVREAR
                     RILAQQEANRMVDFDAVVEAAIKRVEDAAVVFIDEIDKTISGEGGVGPDVSGEGVQRD
                     LLPIVEGSAVMTRYGPIHTDHILFIAAGSFSKSRPSDLIPELQGRFPIRVELQSLSED
                     DLFSILTEPQNALTKQYEALLATEGLTVVFHEEGLREVARLANQVNQRTEDIGARRLQ
                     TILEKVLEEVSFEAPDLAGTTAEIGKAFVLERVGEVVDDDELSNFIL"
     gene            2264..>2673
                     /locus_tag="VFQ54_09630"
     CDS             2264..>2673
                     /locus_tag="VFQ54_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0165293.1"
                     /translation="MIGVDLGGTRIRVALISESGTMEHRLDALSRTEEGALAVVRRIG
                     ELVGRLAAEAGVDPGVPVGIGSPGPLNPRTGEVLYTPNLPGWRNVPIVRLLEEQTGRR
                     VTLANDGNCGTLGEMQFGSAKGVSDLVYIALGTGV"
BASE COUNT          511 a          809 c          930 g          423 t
ORIGIN      
        1 gaatgaacag gggtggcggc cacacgccac aacgatcctc ggcgtcaagc ggggggattc
       61 catcgcactg gccggtgatg gccaggtcac gatgggagac atcgtgctca agcacggtgc
      121 gcggaaactt cgtaccctcc acgacggcgc ggtcgtcgcg gggtttgcgg gagcagtcgc
      181 cgatgcgttg acgctcttcg cgaagttcga ggcccagctc caggagtggg gcggggatct
      241 ccgccgcgcc tcggtcgagt tgaccaagga gtggcgaagc gatcgctacc tccgccggct
      301 cgaggcacag ctgatcgtgg ccgacggtga gtcgatcctc gtcctctcgg gcgagggtga
      361 cgtcatcgag cccgacgacg gcgtgctcgc gatcggctcg ggtgcgccgt atgccacggc
      421 cgccgccaag gcgctcctca ggcatacgga aatgagcgcc gaggagatcg tgcgcgcggc
      481 gatggcggtc accgcggaaa tctgcatctt tacgaacgat catgtgatgg tacaagtggt
      541 cagggccgac gccgacgtgg cggaggagcc attcctgatc ggtcccggat cggaatccgc
      601 gatggataca tccgaggatc cgctgacgga cagcacgacc accggtgacg atgagacaga
      661 agaggacgag gcttgatggc cgaacccgta cgccgcacaa ctggcaagga caagtcggta
      721 cggattggtg gtggcgattc cgagaccgaa cgaccggcgg acagcctcac gccaaaggaa
      781 atcgtcgccg agctcgatcg ctacatcatc gggcaggatg aggcgaagcg cgccgtggcc
      841 gtggcgctcc gcaatcgcta ccggcgccag cagctgagcg acgacctgag acaggaagtc
      901 cagccgaaga acatcctgat gatcggatcg acgggcgtcg gcaagaccga gattgcacgc
      961 cgcgtggcgc gcatcgtcga tgcgccgttc atcaaggtcg aggccacgaa gttcaccgag
     1021 gtcggctacg tcgggcgcga cgtcgagtcg atcgtgcgcg acctcgtcga gatcgcgatc
     1081 gcgatgatgc acacggagca gctcgagaac gtgcgggacg aggccgccaa cgcggcggtc
     1141 gaacggctgg ccgacatcat gtcggagcag atgatccagc aacggccgat ccgtccccat
     1201 cgcaagaacg ggcatgagcc ccaggaagac gaacaggaga tcgcccttcg ggaagcgcgt
     1261 tcggtcgaac gccgccggcg ccgcgaacac aagcggatcg tcgatctcct caaccagcag
     1321 gcaatcgacg acgagacggt cgagatcgag ttcgatcccg acatggactt cgaggagggt
     1381 ggcgcgttcg agttcgcccc gggaggcaat cccgacgagt tccaggagac cttctacgac
     1441 ttcctcgaca gcatgatgcc gcgacgccgc atgcgtcgcc gcgtatcggt gcgcgaggcg
     1501 cggcggatcc tggcgcagca ggaagccaat cgcatggtcg acttcgatgc ggtcgtcgaa
     1561 gcggcgatca agcgggtcga ggacgctgcc gtcgtcttca tcgacgagat cgacaagacg
     1621 atctccggtg agggcggggt cgggcctgat gtttccggtg aaggggtgca gcgcgatctc
     1681 ctgccgatcg tcgagggctc cgcggtgatg acgcgctatg gtccgatcca caccgatcac
     1741 atcctcttca ttgccgccgg gtcgttctcg aagtcgcgac cgtcggatct gatcccggaa
     1801 ctgcagggcc gtttcccgat ccgggtcgag ttgcagtcac tctccgagga cgatctcttc
     1861 tcgatcctga ccgagccaca gaacgcgctg acgaagcagt acgaggcgct gctggcgacc
     1921 gaggggttga cggtcgtctt ccatgaggag ggcctccggg aggtcgctcg cctcgccaac
     1981 caggtcaatc agcgaaccga ggacatcggc gcgcgacgcc tgcagacgat tctcgagaag
     2041 gtcctcgaag aggtctcgtt cgaggcaccc gatctcgcgg gaacaacggc cgaaatcggc
     2101 aaggcgttcg tgctcgaacg cgtcggcgag gtcgtggacg acgatgagtt gagcaacttc
     2161 atcctctaga cggtacgatc ggaactattc gacgtgagac gggcgtggca acgatcgcca
     2221 cgcccctagc gaatgggcgg gcatcgacgt gggcgaacgg aaggtgattg gggttgatct
     2281 tggcggcacc cggatccggg tcgcgctgat ctccgagtcg ggcacgatgg agcaccggtt
     2341 ggatgcgctc agtcgcaccg aggaaggcgc gctcgcggtc gtccgccgga tcggtgagtt
     2401 ggtcggacgc ctcgcggcgg aggcgggcgt cgatcccggc gttccggtcg ggatcggctc
     2461 gccgggcccg ctgaatccgc ggaccgggga agtcctctac acacccaacc tcccgggttg
     2521 gcgcaatgtc ccgatcgtgc ggttgctcga ggagcagacg gggcgtcgcg tcacactcgc
     2581 caatgacggc aattgcggaa cccttggcga gatgcagttc gggagtgcga agggggtcag
     2641 cgacctcgtc tacatcgcgc tcggaacggg cgt
//
LOCUS       DASTMD010000653         3899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2024130, whole genome shotgun sequence.
ACCESSION   DASTMD010000653 DASTMD010000000
VERSION     DASTMD010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024130"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFQ54_09635"
     CDS             complement(<1..599)
                     /locus_tag="VFQ54_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU0165294.1"
                     /translation="MAILDLIQYPNEASNELVHRIPEGGAGEFKLGSQLVVRESQQAV
                     FYRDGKALDVFGPGRHTISTNNIPLLTGLISIPFGGDSPFTAEVYFVSMREFTDMKWG
                     SAQPLVYRDTELGMVRLRAFGTYSIRVKDPQLLVNQFVGNRGSYTTSAAEDYLRSLIL
                     SEFNELLGAMQTSILDLQTVTKQLSDSSRAALADDFARIG"
     gene            complement(680..1414)
                     /locus_tag="VFQ54_09640"
     CDS             complement(680..1414)
                     /locus_tag="VFQ54_09640"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEU0165295.1"
                     /translation="MGILDRISTLVRANVNDLLDKAEDPEKMLDQILRDMQTNQVTAR
                     QQVASMIAQEKELEAELAETQKLSAEWGEKARRAVAAGKDDLAREALRRKKDNDENTA
                     VYQQQYDIQLQTVEKLKQQLQLLDSKYQTTLSHRDSLIARQRRAKATAQVAKSANSAA
                     ASLNPLDASSELDRMERKIRGNEAQAQALLELDNNSFDTQFRELDYDVDIEKELEALK
                     SGSSAPSPALAAGDTAAADDKAPASN"
     gene            complement(1429..2364)
                     /locus_tag="VFQ54_09645"
     CDS             complement(1429..2364)
                     /locus_tag="VFQ54_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165296.1"
                     /translation="MVERVTNLLNRFPGYTGYRDKERRRDEDKRIRESTADSLVQVVD
                     ALTSANAALVAKRDFAQVSAVEQLIARTRTLADRIRTTSYGYAGLFSDRPIDEPVLDQ
                     LRQFDVALQTRVQAMANSGSASSTTGDGLAATGVAIDQMTTLFNARSNVVETAQPTRE
                     KAVLDLLDTAEPPKPSSFASLHRGDTFAVLGDNFQVEATVLLDSGINLARVSDTDGEH
                     AIWFGGGATAQVPSIKLVEQAADAVPPAVTLQGATARVESEKGKQDKAAVSYAYIPGA
                     DDKVEFILTLAGKTRYYIGNLVNDLDVEVFGSGDK"
     gene            complement(2396..2917)
                     /locus_tag="VFQ54_09650"
     CDS             complement(2396..2917)
                     /locus_tag="VFQ54_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165297.1"
                     /translation="MTSSGRTVGFILIIAGAIIGIGISAWLISSINEGNLHASGAILG
                     FILLFGVVVLPMLGGGTFLVIRGNAEAKQMVQINEQRALIDIVKTRGQVQISDLVLEL
                     KSTTDRVQQNLHQLVGRGLFSGYVDWNKGVLYSVEAKELTGRQTCPNCGGQLELAGKG
                     LIKCPYCGAEIFL"
     gene            complement(3060..>3899)
                     /locus_tag="VFQ54_09655"
     CDS             complement(3060..>3899)
                     /locus_tag="VFQ54_09655"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU0165298.1"
                     /translation="AHVVPVLHPRSVVTIHDLGYLHFPDAHTPQRVRSLDLSTRWSIH
                     AAGRIIVPSSFTRDDLIARYAVSPDRIEVVHHGVDPGFRQERPGEVERLRSKHGLARP
                     FVLSVGTIQPRKNFPFLARAVRRLAALGIDCDLVIAGKPGWLADQVLRELDGVGLGDR
                     LRMLSYVADDELPALYRSAACFVLPSRFEGFGMPVLEAMASGTPTIATESSSLPEVVG
                     TGGVVVPLDDDEELALAIARVLTDPAWSSVMRARAIARSMDFTWERSASGTLAVLKAA
                     LAG"
BASE COUNT          682 a         1230 c         1249 g          738 t
ORIGIN      
        1 ccgatccggg cgaagtcatc cgcgagcgcg gcgcgtgaac tgtccgagag ctgcttggtg
       61 acggtctgga ggtcgaggat cgatgtctgc atcgcaccga ggagctcgtt gaactccgag
      121 agaatcaggc tgcgcaggta gtcctccgcg gcggacgtcg tgtagctgcc acggttgccg
      181 acgaactggt tgaccagcaa ctgcggatcc ttgacgcgga tgctgtatgt cccgaacgcc
      241 cgcaaccgga ccattcccag ctcggtgtcg cgatagacga ggggctgcgc cgagccccac
      301 ttcatatcgg tgaactcgcg catcgagacg aaatagacct cggcggtgaa cggggaatca
      361 ccgccgaagg gaatgctgat cagtcccgtc agcagaggga tgttgttcgt cgagatcgtg
      421 tggcggcccg gtccgaagac gtcgagcgcc ttgccatcgc ggtagaagac cgcctgctgg
      481 ctctcgcgaa cgacgagctg cgatccgagc ttgaactcgc cggcgccgcc ttcaggaatg
      541 cggtgaacga gctcgttcga cgcctcgttg gggtactgga tgagatcgag aatggccatc
      601 cggagctccg tttcttgcct gatacggaag acggggagcc gcgccggtcc ggcacgactc
      661 cccgagcgac gcgtgaagcc tagttcgagg cgggagcctt gtcatcggcg gcggcagtat
      721 caccagccgc gagggcgggc gacggagccg aggatcctga cttgagggcc tcgagttcct
      781 tctcgatgtc gacatcgtaa tccagctcgc ggaactgggt atcgaacgag ttgttgtcca
      841 gctccagcaa cgcctgggcc tgcgcctcgt tgccgcggat cttgcgctcc atccgatcga
      901 gctcggagga tgcgtccagc gggttgaggg atgccgccgc ggaattcgcc gacttggcga
      961 cctgggcagt ggccttggcg cgacgctggc gcgcgatcag gctgtcgcga tggctgagtg
     1021 tcgtctggta cttcgaatcg agaagctgca gctgctgctt cagcttctcg accgtctgca
     1081 gctgaatgtc gtactgctgc tggtacaccg ccgtgttctc gtcgttgtcc ttcttgcggc
     1141 ggagcgcctc gcgagcgaga tcgtccttgc cggcagcaac ggcgcggcgc gccttctcgc
     1201 cccactcggc ggagagcttc tgcgtctcgg ccagttcggc ctcgagttcc ttctcctggg
     1261 cgatcatcga cgccacctgc tggcgggccg tgacctggtt ggtctgcatg tcgcgaagga
     1321 tctggtccag catcttttcc ggatcttcgg ccttgtcgag gagatcgttg acgttcgcgc
     1381 ggacgagtgt cgagatgcga tcgagaatac ccatggttga ccctcctgtt acttgtcgcc
     1441 ggagccaaag acctctacat cgaggtcgtt taccaagtta ccaatgtagt atcgcgtctt
     1501 tccggcgagg gtgagaatga actcgacctt gtcatccgct cccggaatgt acgcatatga
     1561 aaccgcggcc ttgtcctgct tgcctttctc gctttcgacg cgcgctgtag cgccctgcaa
     1621 cgttaccgcc gggggcaccg cgtccgcggc ctgctcgacg agcttgatcg atggcacctg
     1681 ggcggtcgcg cccccaccga accagatcgc atgctcgccg tcggtgtcgc tcacccgcgc
     1741 gagattgatt cccgagtcaa gcaagaccgt ggcctcgacc tggaagttgt ccccgagcac
     1801 cgcaaaggtg tcgccacgat gcagcgacgc aaacgaactc ggcttcggag gctcggccgt
     1861 gtcgagaaga tcgaggaccg ccttctcccg ggtcggttga gccgtctcga cgacgttcga
     1921 ccgcgcgttg aagagcgtcg tcatctgatc gatcgccacg cccgttgcgg cgagaccgtc
     1981 gccggtcgtg gacgaggccg agccgctgtt ggccatcgcc tggacgcgag tctgcaacgc
     2041 gacgtcgaat tgccggagct ggtcgaggac gggctcgtcg atcggcctgt cgctgaagag
     2101 accggcatag ccgtacgacg tcgtgcgaat gcgatcggca agcgttcgcg tccgggcgat
     2161 cagctgttcg accgccgaga cctgcgcgaa atcccgcttc gcgacgagcg cggcattggc
     2221 ggaagtcagc gcgtcgacga cctgcacgag gctgtcagcg gtgctctcgc ggatgcgctt
     2281 gtcctcgtcc cgccgtcgtt ccttgtcgcg gtagcccgtg tatcccggaa accggttcag
     2341 caggttggtg acacgttcaa ccattcatga ctcctggcgg atgatcggac cgccgttaca
     2401 ggaagatctc cgctccgcaa taggggcatt tgatcaggcc ctttccggcg agttccagct
     2461 ggccgccgca attggggcag gtctggcgtc cggtaagttc cttagcttct acggaataca
     2521 ggacgccttt gttccaatcg acataaccgg agaacaggcc gcgcccgacc agttgatgca
     2581 gattctgctg gacacggtcg gtcgtggatt tcaactcgag gacgagatcg gagatctgga
     2641 cctggccacg cgtcttgacg atgtcgatca gtgcacgttg ctcgttgatc tgcaccatct
     2701 gcttcgcctc ggcgttgccg cggatcacga gaaaggtgcc accgccaagc attggcaaca
     2761 ccacgacgcc gaagaggagg atgaacccca gaatggcgcc gctggcgtga agatttccct
     2821 cgttgatgct tgagatcagc cacgcgctga tcccgatgcc gatgatcgca ccggcgatga
     2881 tgagaatgaa gccgacggtg cggcccgaac tcgtcacact cgtacccctt gtcgcgttgc
     2941 gtcaccggaa cccgagtgcc tctgggacat tgacctcagc ctccttacct atcgcctgta
     3001 tctcttcgac gtctcgatgc attgtacttc gccggccggt ttggcatgcg tcatcgtcgt
     3061 catccggcca acgccgcctt caatacggcc agtgtcccgc tcgccgagcg ctcccaagtg
     3121 aagtccatcg agcgcgcaat cgccctggcg cgcatcaccg atgaccacgc gggatccgtt
     3181 aggactctcg cgatcgccag tgcgagttcc tcatcatcat ccaacggcac gacaacaccg
     3241 ccggttccaa cgacttccgg cagtgatgac gactccgtgg cgatcgtcgg cgtgccggaa
     3301 gccatcgcct cgagcaccgg catgccgaat ccctcgaagc gggacggcag cacgaagcag
     3361 gcggccgagc ggtagagcgc cggaagctcg tcgtcggcaa cgtacgacag cattcgcagc
     3421 cgatcgccga ggccgactcc atccagctca cggaggacct gatccgcgag ccagcccggc
     3481 ttcccggcaa tcacgagatc gcagtcgatc ccgagggcgg cgagacgcct gaccgcgcgg
     3541 gcgagaaatg ggaagttctt tcgcggctgg atcgtcccca ccgagagcac gaagggtcgc
     3601 gccagtccgt gcttcgagcg gagacgttcg acttcccccg ggcgctcctg gcggaacccc
     3661 gggtcgacgc cgtggtggac gacctcgatc ctgtccggcg acaccgcgta gcgcgcgatc
     3721 agatcgtccc gcgtgaacga ggagggcacg atgatgcgcc ccgcggcgtg gatgctccag
     3781 cgggtcgaca ggtccagcga gcggacgcgt tgcggcgtgt gggcgtcggg gaaatggaga
     3841 tatccgagat cgtggatggt caccaccgat cgtggatgca gaaccggcac gacgtgagc
//
LOCUS       DASTMD010000654         1518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1936103, whole genome shotgun sequence.
ACCESSION   DASTMD010000654 DASTMD010000000
VERSION     DASTMD010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936103"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /gene="pgeF"
                     /locus_tag="VFQ54_09660"
     CDS             <1..756
                     /gene="pgeF"
                     /locus_tag="VFQ54_09660"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEU0165299.1"
                     /translation="HGLTHRVPGMGKAHGNIGYSGDRDREDAWEMRGLWAEAIGFDIE
                     RMVNVGQVHGNEVIRAYADDAGRGARPDSPVIGRADSLITDAPGIVLSTMGADCLPIL
                     LVDPKRPAIAAVHAGWRGTVANVAGNTVRAMQREFGTDPAELIAYLGPAIGVCCNEVG
                     DEVIDAWREQAHDIGPLVELAMIRPRVKQHFDVPRANSLLLQRAGLRPEHMEISQICT
                     KCSTDSWFSHRGHGPTAGRQAAFIGIDAVGSKA"
     gene            753..1475
                     /locus_tag="VFQ54_09665"
     CDS             753..1475
                     /locus_tag="VFQ54_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU0165300.1"
                     /translation="MTDQATQSDATEHAAAVARVRDVQDRVYRAAGLAGRDAESVTIV
                     GVSKTFPRELVDAAYEAGLRVFAESRVQEAREKFATPLPDDASLHMIGQLQTNKVKQA
                     VGVFSCIESVDRANLIESLANEAARRDLVVPILLQVNIAREPQKSGCDPDDVNALVDL
                     IASRSSLRCDGLMTIAPLVVDPEDARLVFRDLRLLRDRLRERHPGMPLEMLSMGMSGD
                     FEVAIAEGATHVRIGRALFGAR"
BASE COUNT          262 a          488 c          517 g          251 t
ORIGIN      
        1 cacgggttga cccatcgcgt ccctggcatg ggcaaggcgc acggcaatat cggctacagc
       61 ggcgaccgcg atcgcgaaga cgcctgggag atgcgcgggc tctgggcgga ggcgatcgga
      121 ttcgatatcg agcggatggt caacgtcggg caggttcacg ggaacgaggt gatccgcgcg
      181 tacgccgatg acgccggtcg tggcgcccgt ccggatagtc cggtgatcgg tcgcgccgac
      241 tccctgatca cggatgctcc cgggatcgtg ctctcgacga tgggtgccga ttgcctgcca
      301 atcctcctcg tcgatcccaa gcgtcccgcc atcgccgccg tacacgccgg atggcgggga
      361 acggtggcga atgtcgcggg aaacacggtc cgggcgatgc agcgcgagtt tggcaccgac
      421 ccggcggaac tcatcgccta tctcggtccg gcgatcggcg tctgctgcaa tgaagtcggt
      481 gacgaggtga tcgacgcctg gcgcgaacag gcgcacgata tcggaccgct tgtcgagctg
      541 gcgatgatcc ggccccgtgt caagcagcat ttcgacgtgc cgcgcgcgaa ttcactgctg
      601 ttgcagcgtg ccggcctgcg cccggagcac atggagatct cccagatctg caccaagtgc
      661 tcgaccgatt cctggttctc ccatcgcggg cacggtccga ccgccggtcg acaggcggcc
      721 ttcatcggga tcgacgcggt tgggagcaag gcgtgaccga tcaggccaca cagtccgatg
      781 cgaccgaaca cgcggccgcg gtcgcccggg tccgcgacgt ccaggatcgc gtgtatcgtg
      841 ctgccggact ggccgggcgc gacgcggaat cggtgacgat cgtcggggtc agcaagacct
      901 ttccccggga gctggtcgac gcggcgtacg aagcaggcct ccgggtcttc gcggagagcc
      961 gggtacagga ggcgcgcgaa aagttcgcca ctcccctccc cgatgacgcc agtctccaca
     1021 tgatcggtca gctgcagacg aacaaggtga agcaagccgt cggcgtcttc tcctgcatcg
     1081 aatcggtcga ccgcgcgaac ctgatcgaat cgctggcgaa cgaggccgcg aggcgcgatc
     1141 tcgtcgtgcc gatccttctc caggtgaata tcgcgcgtga accccagaaa tccggttgcg
     1201 atcccgacga cgttaacgcc ttggtcgacc tcatcgcgtc ccgatcgagc ctccgctgcg
     1261 acggcctgat gacgatcgca ccgcttgtgg tcgatccaga ggacgcacgt ctggtcttcc
     1321 gcgatctgcg cctgcttcgg gaccggctgc gggaacgaca ccccgggatg ccgctggaga
     1381 tgctctcgat gggcatgagc ggcgacttcg aggtcgcaat tgccgagggc gccacgcacg
     1441 tccggatcgg acgggcactc ttcggcgcac gctgacacgg ggatgagggg cgagatggtg
     1501 acgaacgata ccgatcgg
//
LOCUS       DASTMD010000655         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2738924, whole genome shotgun sequence.
ACCESSION   DASTMD010000655 DASTMD010000000
VERSION     DASTMD010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2738924"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFQ54_09670"
     CDS             complement(<1..226)
                     /locus_tag="VFQ54_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165301.1"
                     /translation="MVFDGKVSIEICKRPETIIGFNVMPRRIVHPDPPLSLPGPAATR
                     RQRHPCANHMPDSGGAYIRQDGASSPRWLPD"
     gene            230..2017
                     /locus_tag="VFQ54_09675"
     CDS             230..2017
                     /locus_tag="VFQ54_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU0165302.1"
                     /translation="MIGNGSTAALVARDGTIPWLCIPRFDSEPLFASMLDREIGGAFR
                     ISAGRILGARHRYLGDSAILITELRVDGGLLRITDLMPFRGGVDLAAEGNPETGELLR
                     CVDVIEGSLHIEMELTAHGGVEAERHPKGMRLVVNRSVENEFVLECSRELRGAGGAWD
                     LDEGESVIFCLRWNGGAAASSVDAPFAAITNTIEGWLDWLRAFRYHGPQRRLVRRAAI
                     TLKLLDYLPNGALVAAPTTSLPEAIGGPRNWDYRYVWVRDASFAVNALRRIGMNREAW
                     QFLEWVLANFRERKVNVMYTLDGDPLIPERLDDALSGYRGSAPVRWGNGAAAQIQHDV
                     YGEILDCAFQWSNHGGIIGRELWEQLRGLVDRAAEVWNTPDAGIWEVRRPGFVQTYSA
                     GLCHVALDRGIQIANRYHFEADLDRWTASAREIQKAILTDGWDKRLGFLTQGLQDGHL
                     DAAALALPIRRVIPAKHPRMIATAEAIDRHLGAGNGLLYRYLPELSPDGLPGSEGAFV
                     ICGFWMVDNLTMQGRIGEALERFDRLCAHANVLGLLPEEIDPTTGRFLGNFPQAFSHI
                     GMISSGVNLEHALQSRNDQERHRVRNGRS"
     gene            complement(2057..>2301)
                     /locus_tag="VFQ54_09680"
     CDS             complement(2057..>2301)
                     /locus_tag="VFQ54_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165303.1"
                     /translation="RHLYDQELEGIPTSYTDIISIIQSDNRPKYIISTLHPGPFPDYA
                     GLFWQVVSKYYVIETFVDGVPIYRAKSSFTTPPYPK"
BASE COUNT          398 a          686 c          775 g          442 t
ORIGIN      
        1 agtccggaag ccatcttggc gatgatgcgc cgtcctggcg gatgtaggcg ccacctgaat
       61 ccggcatatg gtttgcgcaa ggatgacgct ggcgccgggt ggcggcaggg ccaggcagcg
      121 aaagaggcgg atccggatgc acgatgcgac gaggcatgac attgaaaccg atgatggttt
      181 caggccgctt gcatatctcg atggatacct tcccatcgaa gaccatgggt tgatcgggaa
      241 cggatcgacg gcggcgctcg tcgctcgtga cggcacgatc ccgtggctct gcattccccg
      301 cttcgactcc gaaccgctct tcgcctcgat gctcgaccgc gagatcggtg gagcctttcg
      361 catcagtgca gggaggattc ttggtgctcg ccatcgctat ctcggtgact ccgcgatcct
      421 gatcaccgag ctgcgggtcg acggcggact gctccggatc accgatctga tgccgttccg
      481 cggcggagtc gatctcgcgg cggaggggaa tcccgaaacc ggggaactcc ttcgctgcgt
      541 cgacgtgatc gagggatcgc tgcatattga gatggaactg acggctcatg gcggcgtcga
      601 ggccgagcgg catccgaaag ggatgcgtct cgtcgtcaat cgctccgtcg agaacgagtt
      661 cgttctcgag tgctcgcgtg aactccgcgg cgcgggtggc gcgtgggatc tcgacgaggg
      721 tgaatcagtg atcttctgtc tgcgctggaa tggcggggca gcagcgtcgt ccgtggacgc
      781 gcccttcgcg gcgatcacca atacgatcga gggctggctc gactggctgc gcgcgtttcg
      841 ttaccacgga ccgcagcgcc ggctcgttcg tcgtgccgcg atcacgttga agctcctcga
      901 ctacctcccc aacggcgctc tcgtggcggc cccgaccaca tccctgccgg aagcgatcgg
      961 cggtccgcgc aactgggatt atcgctacgt ctgggtgcga gatgcgtcgt ttgccgtgaa
     1021 tgcgctccgc cggatcggca tgaatcgtga agcgtggcag tttctcgaat gggtgctggc
     1081 caacttccgg gagcgcaagg tcaatgtgat gtacacgctc gacggggacc cgctgatccc
     1141 ggagcgcctc gatgacgcgc tttcgggata tcgcggttcg gcgccggtgc ggtgggggaa
     1201 tggcgcggcc gcccagatcc agcacgatgt gtatggggag atcctcgatt gcgcctttca
     1261 gtggtcgaac cacggcggga tcatcgggag agaactctgg gagcaactgc gggggctcgt
     1321 cgatcgggct gccgaggtct ggaacacccc cgatgccggg atctgggaag tacggcgacc
     1381 cgggttcgtg cagacctatt ccgcgggact ctgccacgtg gcgctcgacc gcgggattca
     1441 gatcgcgaac cgataccact tcgaggccga tctcgatcgt tggaccgcga gtgctcgcga
     1501 gatccagaag gcgatcctga cggacgggtg ggacaagcgc ctcgggtttc tcacccaggg
     1561 cttgcaggac ggtcatctcg atgccgccgc cctcgccttg ccgatccgcc gcgtcatccc
     1621 ggcgaagcat ccccgaatga tcgcgacggc tgaggccatc gaccgtcacc tcggagccgg
     1681 gaacggcttg ctctatcgtt accttccgga gctttcacca gatggcctgc cgggatcgga
     1741 aggcgccttc gtgatctgcg gtttctggat ggtcgacaac ctgacgatgc aggggcggat
     1801 cggcgaggcg ctcgagcgct tcgatcggct ctgcgcccat gcgaacgtgc tggggttgtt
     1861 gccggaggag atcgatccga cgaccggtcg attcctcggg aacttcccgc aggcgttcag
     1921 ccacatcggg atgatctcga gcggtgtcaa cctcgagcac gcgctccaga gtcggaacga
     1981 ccaggagcgt caccgcgtgc gcaacggccg ctcctgatac gacccatcgc ccgaagagac
     2041 gactggtcgg atgacctcac ttgggatagg gcggggtcgt gaacgaactc ttggcgcgat
     2101 agatcgggac gccatcgacg aaggtctcga tcacgtagta cttgctcacc acctgccaga
     2161 agaggcccgc gtagtccggg aatggacccg gatgaagcgt gctgatgatg tattttggcc
     2221 ggttgtcact ctggatgatc gagatgatgt ccgtatacga cgtcgggatg ccctcgagct
     2281 cctggtcgta gagatgacgg t
//
LOCUS       DASTMD010000656         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_60126, whole genome shotgun sequence.
ACCESSION   DASTMD010000656 DASTMD010000000
VERSION     DASTMD010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60126"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFQ54_09685"
     CDS             complement(<1..610)
                     /locus_tag="VFQ54_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0165304.1"
                     /translation="MTSAMSPKEALPNVLTAAEANRERDLADLIRLVAQPSISAQGIG
                     IAECCAIEEELLRSAGLTTRIIETPRQPMVYGEWLGAPGKPTVLFYGHYDVQPPDPLE
                     LWTTPPFEPTIRDGRIYARGVADNKAQHFSHIAAIRAWLEATGSLPINVKVLLEGEEE
                     VGSPNLDSTIVANRDLLTADLVYTSDGPVTDDRFPEIAFGVRG"
     gene            878..>2322
                     /locus_tag="VFQ54_09690"
     CDS             878..>2322
                     /locus_tag="VFQ54_09690"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU0165305.1"
                     /translation="MSNFFRAIARPIRRLYGLLPYVRGLRFRLVMLVLLASLPAFGLL
                     FYTASQQRNDALDSGQEEASSLVNLFAADQLRIVNQNEQYLKTLARLPELRGDDATAC
                     SSLVVALLQDNGYAFQNIGVIHKDGTSTCRASGSLPAYFVDSPDHLAAAIDAGSMTIG
                     NIQTVAGSQDGMVAYAYPVPNASGIVDRIVFVSLPYESLSSFAAQAKPREGTILRVFD
                     NNNILIAQYPNDASNPTTLATPIASTPSALQPNQGTIQDTKGTSYLFARGDIALPSGV
                     QNAGPAVVSVALPRNEIVSRADSLFQQNVGRLAIVAVLAVVAAWVGADLFTAGDSVTR
                     KRIVHEWFDAFSAGNIERISELMSASYVDRSRAPGQAEGIDGIRQVVAAFKTAFPDGT
                     LTVRELLADADKVVARVTLVGTHVGEFFGTQPSGKRVASEGDEIFRFAGGAIVESWSL
                     YGPLVVVNPDPEPEIPLGGPLRRLLRRIVRR"
BASE COUNT          378 a          717 c          788 g          439 t
ORIGIN      
        1 tgccgcggac accgaaggcg atctccggga agcggtcgtc ggtgacggga ccgtccgagg
       61 tgtagacgag atcggcggtc agcaggtcac gattggcgac gatggtgctg tcgagattgg
      121 ggctgccgac ctcctcctcg ccctcgagca gtaccttcac gttgataggc agcgagcccg
      181 tcgcctcgag ccaggcgcgg attgcggcaa tgtggctgaa gtgctgggcc ttgttgtcgg
      241 cgacaccgcg ggcgtagatg cgaccgtcgc ggatggtcgg ctcgaacggc ggggtcgtcc
      301 acagctcgag gggatcgggc ggctggacgt cgtagtgacc gtagaagaga acggtcggct
      361 tgcctggcgc tcccaaccac tcgccgtaga ccatcggctg gcgcggcgtt tcgatgatcc
      421 gtgtcgtgag tccggcactg cggagcagtt cttcctcgat cgcgcagcac tcggcgatac
      481 cgattccttg cgcgctgatg ctcggctggg cgacgaggcg gatgagatcg gcgagatcgc
      541 gctcccggtt cgcctcggcg gcggtgagga cgttgggaag tgcttccttg ggggacatgg
      601 cagaggtcat cggttctcca gagagtcgtg ctggcgacgg tcgggctgag gcgatccggc
      661 ccgcgtcgga cggaaatcct ggttcacatg aagcacgacg tcccggatgc cgtcaagtgt
      721 gacgtgggtg ggtgacagtc cctggcaagt cctcggcccg ggaactgcgt cgtcattggg
      781 gcgacaggcg tcgcgaatcg cgcattctgt ctacactgtg aacaccgcat tcaggttgtg
      841 tgtcttgcgg tgaccgagca ttgctggtgg gacccgattg tcaaacttct ttcgagcaat
      901 agcgcgcccg atccgccgtc tctacggact gcttccctac gtccgtggat tgcggtttcg
      961 gctcgtcatg ctcgttctcc tcgcgtcgtt gccggccttc ggcctgctct tctatacggc
     1021 gtcccagcag cggaacgacg cgctcgattc cggtcaggaa gaggcgagca gtctcgtcaa
     1081 cctcttcgcc gcggatcagt tgcggatcgt caaccagaac gagcagtatc tcaagacgct
     1141 ggcgcggctt ccggagcttc gaggcgacga tgcgaccgcg tgcagcagcc tcgtcgtcgc
     1201 gctgctgcag gacaacggct atgccttcca gaacatcggc gtcatccaca aggatggcac
     1261 gtcgacctgc cgcgcgagtg gctcacttcc cgcctacttc gtggacagcc cggatcacct
     1321 cgcggctgcg atcgatgccg gctcgatgac gatcgggaac atccagacgg tcgccgggag
     1381 tcaggatggg atggtcgcct acgcctatcc cgtgccgaac gcgagcggaa tcgtcgatcg
     1441 catcgtcttc gtttccctgc cctacgagtc gttgagttcc ttcgcggcgc aggcgaagcc
     1501 gcgggaaggg acgatcctcc gcgtcttcga caacaacaac atcctgatcg cgcagtaccc
     1561 gaatgacgct tcgaatccga cgaccctcgc gacgccgatc gcgtcgacac cgtccgcgct
     1621 gcagccgaac cagggcacga tccaggatac gaaggggaca tcgtatctct ttgcccgcgg
     1681 tgatatcgcc ctgccgagcg gggtccagaa cgccgggccg gcagtcgtct ccgtcgccct
     1741 gccgcggaac gagatcgtga gtcgtgcgga cagtctcttc cagcagaacg tcggccgcct
     1801 cgcgatcgtc gccgtgctcg ccgtggtcgc ggcgtgggtt ggcgccgatc tcttcacggc
     1861 aggagactcc gtgacccgca agcgaatcgt gcacgagtgg ttcgatgcct tctcggcagg
     1921 aaacatcgag cggatcagcg agttgatgag cgcgagctat gtcgatcgca gccgcgcgcc
     1981 cggccaggcc gagggaatcg acggcattcg tcaggtggtg gcggccttca agaccgcctt
     2041 cccggacggg acgctcaccg ttcgcgagtt gctggccgac gccgacaagg tcgtcgcgcg
     2101 ggttactctc gtcggcacgc acgtcggcga gttcttcggg acgcaaccga gtggcaagcg
     2161 agttgcctcg gaaggggacg agatcttccg ctttgccggt ggggcgatcg tggagagctg
     2221 gagtctctac ggtccgctgg tggtcgtgaa tccggatcca gagccggaga ttccgctcgg
     2281 aggaccgtta cgacggctcc tgcggcggat cgtgcggcgg tg
//
LOCUS       DASTMD010000657         1804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_684813, whole genome shotgun sequence.
ACCESSION   DASTMD010000657 DASTMD010000000
VERSION     DASTMD010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684813"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..840
                     /locus_tag="VFQ54_09695"
     CDS             244..840
                     /locus_tag="VFQ54_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0165306.1"
                     /translation="MTIEDRRRRDRVAQRQLITATARSLAEREGWDAVTTRRLSAEIE
                     YSQPVIYKHFASLDDLVEAVALEGFSELAESLGLARGTASPDDVVAAVGRAYSAWAVE
                     NPALYDAMFARATRLTFGADDTPAPMSSSYAELRSAVATIAGERDVDTLTEVLWAALH
                     GLITLDRNGRLRPNHDIDRIDVLIAQFRSPPPGRLTSG"
     gene            complement(996..1631)
                     /locus_tag="VFQ54_09700"
     CDS             complement(996..1631)
                     /locus_tag="VFQ54_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU0165307.1"
                     /translation="MTDSGAGDANRRSDLGTLVAHLAELKRIPRTGWLDRGLPSNEAE
                     SVADHSLLAALIAWLTAMDDPDLDADHVLKLALVHDLAEAIFGDLPPYDRAEVPTDPG
                     ERDAFFRVRHLRTPENAAAKRAAESDAAATLLDLMPETARAEFSSLWDEYEARETSES
                     RFVKQVDRLEAFLQARIYAQDHPDLPLDGFTDMAEKEIHHPALTALRDEVE"
     gene            complement(1624..>1804)
                     /locus_tag="VFQ54_09705"
     CDS             complement(1624..>1804)
                     /locus_tag="VFQ54_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0165308.1"
                     /translation="YRAAVTEHHKAHDFIVYPDAGHGFMTFDPTAETYTAAHDSWNRT
                     LEFLGIHLDRPHYRD"
BASE COUNT          290 a          550 c          624 g          340 t
ORIGIN      
        1 ttcgcgcccg tcggggacga atgcgttgtg gttgatgtag tccccggcga cgaagcgatc
       61 gaccatgtcg gggtcgtgcg tgttgatcat ctcgaggaac gcttcggcga gcgcctgccc
      121 gttcgttgcc atgttgcgac cctttctgtg cgagttagca acgctaacca ctagcaatgc
      181 taatcctcgt cgatgctcgc gtcaagagca ttgctcgcac atccagcgtt gctatacttg
      241 ggcatgacga tagaggatcg acggcgccgt gaccgggtcg cccaacgcca acttatcacg
      301 gcgaccgcgc ggtccctggc cgagcgcgag ggctgggacg ccgtcacgac gcggcggttg
      361 tcggccgaga tcgagtacag ccagccggtg atctacaagc actttgcgtc gcttgacgat
      421 ctcgtcgagg ccgttgctct ggaagggttc agtgagctcg ccgagtccct cggcctggct
      481 cgagggaccg cttcaccgga tgatgtggtc gctgccgtgg gccgtgctta cagcgcgtgg
      541 gccgtcgaga atcccgccct ctacgatgcg atgttcgccc gggccacacg gctgacgttt
      601 ggcgccgacg acactccggc gccgatgtcg tccagttacg ccgaactgcg tagcgcggtc
      661 gccaccatcg ccggcgagcg agatgtcgac acgcttaccg aagtcctgtg ggccgccttg
      721 catgggctca tcacgctcga tcgtaacgga cggcttcgtc cgaaccacga catcgatcga
      781 atcgatgtgc tcatcgccca gttccgttcg ccgcctcccg ggcgcctgac gtcgggttga
      841 cgatcggacc ccgcgccagg caatggccga tcccaagcgt ctgacgaaca cggaaacgtg
      901 ccttcactcc actcttgcgg cgaagctgat tatagcggcg gttaaggacc ggcttgcctc
      961 acgccgtgga gtgcatcggc gccgctgttc cagcgtcatt cgacctcgtc gcgaagggcg
     1021 gtcagggcag ggtggtggat ttccttctcc gccatgtcgg tgaagccgtc gagcggcaga
     1081 tccgggtgat cctgcgcgta gattcgcgcc tggaggaagg cttcgaggcg gtcgacctgt
     1141 ttcacgaagc gagactcgct ggtctcccgc gcctcgtact cgtcccagag cgaggagaac
     1201 tccgctcgcg ctgtctccgg catgaggtca agcagcgtcg cggccgcatc ggattcggcg
     1261 gcgcgcttgg cggcggcatt ctcgggcgtg cgcaggtgtc ggacgcggaa gaaggcgtcg
     1321 cgttcgccgg gatcggtcgg gacctcggcg cggtcgtacg gtggcaggtc gccgaagatc
     1381 gcttcggcga ggtcatggac gagcgcgagc ttgaggacgt gatcggcatc gagatcgggg
     1441 tcgtccatcg ccgtgagcca cgcgatcaat gccgccagca gggagtgatc ggccacggac
     1501 tccgcctcgt tggagggaag tccgcggtcg agccacccgg tgcgcgggat gcgcttgagt
     1561 tcggcgagat gggcgacgag cgtgccgaga tcggatcgtc ggttcgcgtc gccggcgccg
     1621 gaatcagtca cggtagtgcg ggcgatcgag gtgaatgccc aggaactcga gcgtccggtt
     1681 ccaggaatcg tgggcggcgg tgtacgtttc cgccgtcgga tcgaaggtca tgaagccatg
     1741 accggcgtcc ggatagacga tgaaatcgtg ggccttgtgg tgctcggtga cggcggcgcg
     1801 atag
//
LOCUS       DASTMD010000658         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_953306, whole genome shotgun sequence.
ACCESSION   DASTMD010000658 DASTMD010000000
VERSION     DASTMD010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953306"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFQ54_09710"
     CDS             <1..198
                     /locus_tag="VFQ54_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165309.1"
                     /translation="PQADSNGNIHLKTRSENGDKRRGDGNGNVSFRRDHEGRPVPTTC
                     CGHVWEATTWVAGTRIERVVP"
     gene            complement(255..1301)
                     /locus_tag="VFQ54_09715"
     CDS             complement(255..1301)
                     /locus_tag="VFQ54_09715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CE1758 family FMN-dependent luciferase-like
                     monooxygenase"
                     /protein_id="HEU0165310.1"
                     /translation="MEFGIFGVGDVTQDPSTGITPSENERIQGLARIAKHAEDIGMDV
                     YAIGEHHNPPFISSADTTLLAYIAAKTERITLSTSTTLITTNDPVKIAEDFATLQHLA
                     GPRVDLMLGRGNTAEVYPWFGQDIRQGIPLAIENYALLRRLWREEKVTWSGQFRAPLQ
                     GFTSIPRPLNGAPPFVWHGSIRSPEIAEQAAFYADGFFVNNLFMTLDYFRQYVEFYRE
                     RFEHYGHGTQDEAIVGAGGAIYVRPNSQDAWKEVAPFYYASPVMSSSGPLEDAVAETG
                     LAVGSPAEIVEQVMKHREVFGRYQRQLFAVELAGIPEKTVHEMLDLIGAEVLPVLRKE
                     FAALDSPAAELATA"
     gene            1429..1920
                     /locus_tag="VFQ54_09720"
     CDS             1429..1920
                     /locus_tag="VFQ54_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU0165311.1"
                     /translation="MTESRWLDEREHAAWRAYVALQTRLTARLGRELKRESELSVPDY
                     AVLFELSEARDGKLRIGELSDRVEWDKGRLSKQLTRMERRGLIVREPCDTDGRGFFAV
                     LTAAGRSAIEASAPLHVGHVRQWFVDLLTPEQMETIVAISNTVVAKLDSDQSLCPAMP
                     DDA"
     gene            2240..2452
                     /locus_tag="VFQ54_09725"
     CDS             2240..2452
                     /locus_tag="VFQ54_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU0165312.1"
                     /translation="MTHTRSLTRFAWLAIVAAIITISFKMFAWRLTGSVGLLSDALES
                     IVNLVTAIAALIALTVAEQEPDNKGT"
BASE COUNT          464 a          799 c          788 g          477 t
ORIGIN      
        1 ccgcaggcgg actccaacgg aaacatccac ctcaagacgc gctccgaaaa cggcgacaag
       61 cgccgtgggg acgggaatgg gaacgtgtcg ttccgaagag atcacgaggg tcgccccgta
      121 ccgacgacgt gctgcggtca cgtctgggag gcgaccacgt gggtcgccgg gacgaggatc
      181 gaacgtgtgg ttccatagac atcggcggag tgcggacggc gacgtccgcc gtccgccgtc
      241 cgccggcgga aggattacgc cgtagcgagc tcggccgccg ggctatcgag ggcggcgaat
      301 tccttgcgga ggaccggcag gacctcggcg ccgatcagat cgagcatctc gtggacggtc
      361 ttctccggga tgccggcaag ttccaccgcg aagagctggc gctggtacct cccgaagacc
      421 tcgcgatgct tcatcacctg ctcgacgatc tccgccgggc tgccgacggc gagccccgtc
      481 tcggcgacgg cgtcctcgag cggacccgag ctcgacatca cgggactcgc gtaatagaag
      541 ggtgcgactt ccttccaggc gtcctgcgag ttcggacgca cgtagatcgc gccgccagcg
      601 ccgacgatcg cctcatcctg cgtgccatga ccgtagtgct cgaaacgctc ccggtagaac
      661 tcgacgtact ggcgaaagta atcgagcgtc atgaagagat tgttcacgaa gaagccgtcg
      721 gcatagaacg ctgcctgctc cgcgatctcg gggctccgga tcgagccatg ccagacgaag
      781 ggaggcgcgc cgttcagcgg acgcgggatc gaggtgaatc cctgcagcgg cgcccggaac
      841 tggccgctcc aggtcacctt ctcctcgcgc cagagccgcc gcaggagcgc atagttctcg
      901 atcgccagcg gaatcccctg ccggatgtcc tggccgaacc acggatagac ctccgccgtg
      961 tttccgcgcc cgagcatcag gtcgacacgc ggtccggcga gatgctggag cgtggcgaag
     1021 tcctcggcga tcttcaccgg atcgttggtc gtgatcaggg tcgtcgatgt ggagagcgtg
     1081 atccgctcgg tcttcgcggc gatgtaggcg agcagcgtcg tatccgcact cgagatgaac
     1141 ggcgggttgt gatgctcgcc gatcgcgtaa acatccatgc cgatgtcctc ggcatgcttc
     1201 gcgatccggg cgagtccctg gatgcgctcg ttctcgctgg gcgtgatccc ggtgctggga
     1261 tcctgggtga cgtcaccgac gccgaagatg ccgaattcca ttgcaattca cctcatatgt
     1321 gggcgccgtg gaccttcttc ccgtcctccg gcgccgcact ggttgtttcc atgtcaacaa
     1381 tatagagaga tatagtttcc atgtcaagta tctgctagaa tcatgtccat gaccgaatca
     1441 cgatggctcg atgaacgcga gcacgccgcc tggagagcat acgtcgcgtt gcagacacgc
     1501 ctgaccgcgc gactcggacg cgagctgaag cgggagagcg aactctcggt cccggattac
     1561 gccgtcctct tcgaactttc cgaggcgcgg gatggcaagc tccggatcgg tgaactcagc
     1621 gaccgcgtgg aatgggacaa ggggcgcctc tccaagcagc tgacccggat ggagcgccgc
     1681 ggcctgatcg tccgcgagcc gtgcgatacc gacgggcgcg gcttcttcgc cgtcctcacc
     1741 gctgccggtc gctccgcgat cgaggcgagc gcgccgctgc acgtcgggca tgtccgccag
     1801 tggttcgtcg atctcctgac gcccgaacag atggagacga tcgtcgcgat ctcgaatacg
     1861 gtcgtcgcga agctcgattc ggaccagtcg ctctgcccgg cgatgccgga cgacgcgtaa
     1921 cccggctcct cgttgtccgg acgcagatcg tcttagccca ctgtccgtcg atggacatcc
     1981 gcccccgaat cgcataaact atgaaaagag tcgcgaagca cgattttcac tgtgcatgcc
     2041 atatgtcaat cacatcaaca ccgcctgcat gactatgtgc aggcggtgtt tcgtctctca
     2101 tcatgaatta gttgttgcta gtcgatcgaa ttcgggcgcg aaacgcctgt cggatgcttc
     2161 gtccgcatct tgcggtagtg atgactaatc cacttcgaaa cctcgtggac gcttgcaggt
     2221 ccaccccagg ggtctggcca tgacacatac ccggtcgctc acacgattcg cctggctggc
     2281 gatcgtcgcc gcgatcatca ccatctcatt caagatgttc gcctggcggc tgaccggatc
     2341 ggtcggtctc ctttccgatg ccctcgaatc gatcgtcaat ctcgtaacag cgatcgccgc
     2401 cctgatcgcg ctcacggtgg ccgagcagga gcccgacaac aagggcacgt gaaagcacga
     2461 cggtcgtcac gctatttttt gccggcgggt ttggtcggcg ggaagggtga gccttgtcac
     2521 atcttatg
//
LOCUS       DASTMD010000659         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1936192, whole genome shotgun sequence.
ACCESSION   DASTMD010000659 DASTMD010000000
VERSION     DASTMD010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936192"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(827..1780)
                     /locus_tag="VFQ54_09730"
     CDS             complement(827..1780)
                     /locus_tag="VFQ54_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU0165313.1"
                     /translation="MIGRSSDRMSRRSLVRASAALGAGTLALGASRGPAFAQPASPAA
                     TPDVSLSYARPDLLAAPGTIPEDHSLVALMSAATYAAGHIAVSSQIDWSTLNLADTSD
                     AAIADWDGSVRAALAPIGALSAPVLAYDEGTLFAARLWWVLFYLGYPTAAVLNGGLPA
                     WVAAGKPVVTGDTGLPAPLVATPAPESVPAVRRDALATLTDVEGILGNKNVVILDARA
                     PSEYEAGHIPGAVNLEYTKNAASMSPPLWKPASELMEMYAAIGVTPDKKIVPYCSTGV
                     RSAVTFFTLWLLGFPQVGLYTGSWQEWGANPKTPKTKGAEP"
     gene            complement(1838..2401)
                     /locus_tag="VFQ54_09735"
     CDS             complement(1838..2401)
                     /locus_tag="VFQ54_09735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU0165314.1"
                     /translation="MPQKQIEVAAIVGSLRRQSFNRGLLRSAMLTEPEMMHIYEVPIA
                     GIPFFDEDLERQGDPPTVRDLKRALERASAVLIVTPEYSYSIPGVLKNALDWASRPAG
                     RSAIRGKPVAMMGASVGRSGTMRAQLHLRQILVQLGAIPIPEPEIYLTFAGDKFNSLG
                     ELTDKSTLDQTRQMMANLYDWATMLGS"
BASE COUNT          459 a          886 c          879 g          494 t
ORIGIN      
        1 attcccgccc ccgaccatga tcgcgacctg gaccccggct tcggtgagcg actggatttc
       61 atcgacgagg gcgtcgaccg cggcacgatc gatgccggtc tccgccgcac cggagaaggc
      121 gccgccgctg agcttgataa ggacgcgacc atacttcacg attgttgcct ttccatcagg
      181 cgcgacatgt cgcgggctcg tgccgcgcgc attccgttcc ggactctata gcaggcggaa
      241 gcggccctgc aaggttttgg gagagatgta actcccgggt ccgtcaggtc atggcccgac
      301 gagcgtcatc gtcgcctcca gatcggcgtg cgctcattcc cgtccccatg gcccttgtgg
      361 ccgtttcccc tacgtccgtc gcgaggcgtc ggaaacttcg gaacacgtcc tcccgaatgg
      421 agcgccgatg tggtgccgtc aggcgcgcgt ccgtaggggc gatcccggtg atcgcccggg
      481 atcacgtccc atgcccgatg ccttgccggc gtgcccttct ggcggcggtg ttggaagcac
      541 gcggtgccca actggtccct cgacaagctc gggaggacgt gttgtgtgcg gtgtcgtgag
      601 ggaagcaaag gagtgccggc acggctacag cgaaatccga aacggttgct gaagatgatg
      661 ctgggccgaa tgcgcattcc gcggtgcgac cggtcgttcg ggagcctgtc ctgagcttgt
      721 cgaaggagcg ggaccgccgc tgccggaccg gaggtccgga cggcctgtcc cgcctcccgt
      781 tcgcccacgg atcgtgctcc ccgttgcgac gatcccggat ttcgctctag ggttcggcgc
      841 ccttcgtctt tggcgtcttt ggatttgcac cccactcctg ccatgaaccc gtgtagagcc
      901 cgacctgcgg gaatcccagc agccagagtg tgaagaaggt gaccgccgac cgcacgcccg
      961 tcgaacagta ggggacgatc ttcttgtccg gggtgacacc gatcgccgcg tacatctcca
     1021 tgagttcgga ggcgggtttc cagagcggag gcgacatcga cgccgcgttc ttcgtgtatt
     1081 cgagattgac agccccggga atgtggccgg cctcgtactc cgatggagcg cgtgcatcga
     1141 ggatgacgac gtttttgttc ccgagaatcc cctcgacgtc ggtcaaggtt gccaacgcat
     1201 cgcggcgaac cgcggggaca gactcagggg cgggtgtcgc gacaagcggc gcggggagtc
     1261 ccgtatcgcc ggtgacgacc ggctttcccg cggcgaccca cgccgggagg ccgccgttga
     1321 gaacggccgc ggtgggatag cccagataga agagcaccca ccagagacgc gccgcgaaga
     1381 gcgttccctc gtcatatgcg agtacgggag cggagagtgc gccgattggc gccagcgccg
     1441 cgcggacgct tccgtcccag tccgcaatcg cggcgtccga cgtatcggcg aggttcagcg
     1501 tggaccagtc gatctggctc gataccgcga tatgccccgc ggcgtaggtc gcggcggaca
     1561 tcagcgccac gagggagtgg tcctcgggga tcgtgcccgg tgccgcgaga aggtcgggac
     1621 gcgcgtagga aagtgacacg tccggcgtgg cggccgggga tgccggctgc gcgaacgccg
     1681 gtccccgcga tgctccgagc gccagcgtcc cggcgccgag cgccgctgac gcgcgaacaa
     1741 gactccgtcg actcatccga tcgctgctac gtccgatcat ctcagcctcc gttccgggga
     1801 tcacccttcg accatcagct tacgtgccac gagcggatca ggatccgagc atcgtggccc
     1861 agtcatacag attcgccatc atttgccgcg tctgatcgag cgtcgacttg tccgtcagtt
     1921 cgccgagcga attgaacttg tcgccggcga aggtgaggta gatctccggc tccggaatcg
     1981 ggatcgcgcc aagctgcacc aggatctgtc gcaggtgcaa ttgcgcacgc atcgtgcccg
     2041 atcgcccgac ggacgcgccc atcatcgcca ccggtttccc ccggatcgcc gatcgtcccg
     2101 ccgggcgtga tgcccagtcc agcgcattct tgagcacgcc ggggatcgaa taggaatact
     2161 cgggggtcac gatcagcacc gcgctcgcgc gctcgagcgc acgcttgaga tcgcggacgg
     2221 ttggcggatc gccctgacgc tcgagatctt catcaaagaa cgggatgccg gcgatcggga
     2281 cctcgtagat atgcatcatc tctggttccg tcagcatcgc cgaccgcagc aatccccggt
     2341 tgaacgactg gcgacgcagg ctgcccacga tggcggcaac ctcgatctgt ttctggggca
     2401 cgataatcct tttcctcgtg ggatgacgta gtccgtccgg ttgtgccgtc gtgcgatgta
     2461 ccgtcccacc cccgccggtg gatgcatgtg tcgtcacgac cagtcaatca atggcacatt
     2521 ttggtccacg ccatgttctt cctggctttc gcccggcaat cttcgcatac gaaccgcaac
     2581 acgacagcac cccggtcgtc cagggacaac cggggtgctc gtggtgatga tcgcccgaat
     2641 gcgaaccggc ggttacgcct gcatcaatcg ccggagcacg gtcggcagaa tgccgccgtt
     2701 ccggtaatag cggacatc
//
LOCUS       DASTMD010000660         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2651783, whole genome shotgun sequence.
ACCESSION   DASTMD010000660 DASTMD010000000
VERSION     DASTMD010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2651783"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VFQ54_09740"
     CDS             complement(<1..1596)
                     /locus_tag="VFQ54_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU0165315.1"
                     /translation="MEDVRIILNLFIALGAAVAGGLIARRLGLPVLIGYVVAGIVIGP
                     NSPGVVADTGQVTLLANLGVAFLMFALGVEFSIGHLLEVRRIAIVAASVQYPVTFALG
                     ALVGRAIGWDWAASLLLGGVFVISSSIVMIKLLINRGETTSWHARIAFGLGVIQDLSL
                     VPLLAFVPVLSGESDHLVGSLAKSLGTAALALVLVFALGLKLVPFVLYRIARTGSREL
                     FLLIIVVIAMGTAYASHEAGLSFALGAFLAGLVVSESEFATEVLAEIVPLRDLFSTLF
                     FVSLGMLLRPELFLDHPVEIGLSLLALVVGKIAIAAAGFLVAGIEITSALLAALYLAQ
                     IGEFSFVLAGVGLDDDILDNDQYGIILTVALVSILISPLLPLLEPRLAPIVGRIPVVR
                     RRETRDAVEDQDHAAFRRHVIIAGYGRVGQALGDALLRKGLRFVVVDLNPGIIRDLRT
                     REIPAIYGDGATEAVLEKAGIGSARALAITIPDPIAARGMTARARKMSDTVDIVTRAG
                     YHDNVDELVAVGANETVQPEFEAG"
BASE COUNT          448 a          645 c          656 g          308 t
ORIGIN      
        1 ccccgcctcg aactcgggct ggacggtctc gttggcgccg acggctacca gctcgtcgac
       61 attatcgtgg tatcccgccc gcgtcacgat gtcgaccgtg tcactcatct tccgcgcccg
      121 tgccgtcatt ccccgcgccg cgatcggatc gggaatcgtg atcgccagtg cacgcgccga
      181 gccgatccca gccttctcga gcaccgcctc agtggcgccg tcgccataga tcgccggaat
      241 ctcgcgcgtc ctgaggtcgc ggatgatgcc cgggttcagg tcgacgacga cgaaccgcag
      301 tcccttgcgg agcagggcat cacccagcgc ctgcccgaca cgaccgtacc ccgcgatgat
      361 cacatgccgg cgaaaggcgg cgtgatcctg atcctccacc gcgtcccgcg tctcgcgccg
      421 gcgaacgacg ggaatccgac cgacgatcgg ggcgagacgc ggctcaagga gcgggaggag
      481 cggcgagatc aggatcgaga cgagcgcgac ggtgagaatg atgccgtact gatcgttatc
      541 gagaatatcg tcatcgagcc ccacccccgc caggacgaaa ctgaactcgc cgatctgagc
      601 gagatagagg gccgccagaa gtgccgacgt gatctcgatg ccggctacga gaaatcccgc
      661 agccgcgatc gcgatcttgc ccaccacgag cgcgaggagg ctgagcccga tctcgaccgg
      721 atggtcgagg aagagttccg gccggagcag catcccgagc gagacgaaga agagcgtgga
      781 gaaaagatcg cgcaggggca cgatctcggc gaggacctcg gtcgcaaatt ccgattcgga
      841 aacgacgagc ccggcgagga atgcaccgag ggcgaacgaa agcccggcct catgagaggc
      901 gtacgcggtt cccatcgcga tgacgacgat gatcagcagg aagagttcgc gcgatcccgt
      961 ccgggcgatc ctgtagagaa cgaacgggac gagcttcagg ccgagggcaa agacgagtac
     1021 gagcgcaagc gccgccgtcc ccagcgactt tgccaacgag ccgacgagat ggtcggactc
     1081 gccactgagg accggcacga aggcgagcag cggcacgaga ctgagatcct ggatcacacc
     1141 gagcccgaac gcgatccggg cgtgccaact cgtcgtctcc ccccggttga tcagcaactt
     1201 gatcatcacg atggaactgg agatcacgaa gacgccgccg agcaggagcg aggccgccca
     1261 gtcccatccg atcgcgcggc cgacaagggc tcccagggca aaggtcacgg gatactgaac
     1321 cgacgcggcg acgatcgcga ttcgccggac ttccagcagg tgcccgatcg agaactcgac
     1381 gcccagcgcg aacatcagga aggcgacacc gagattcgcg aggagggtga cctgtcccgt
     1441 atccgcgacg acgcccgggc tattcgggcc gatcacgatc cctgcgacga catacccgat
     1501 caacaccggc aggccaagac gacgcgcgat caaaccacca gcaaccgcag caccaagcgc
     1561 aatgaagaga ttgagaataa tgcgtacgtc ttccatacat ctcctgtgga aggcatccgt
     1621 tggcaacgta ttccgatgtt gtacgaagcg gggatgacaa agtcagcgtc tggcggcttg
     1681 ggtcaggcga gggcaacgac gaaggttccg caaagggaat ctgcctttat catcgttgat
     1741 tttcggggcg ctgcacaggg aaaccggagc ggatacgaca ttatgactcg acaagtatgc
     1801 gtcggatgga ggatatccag gagatatccc ggttgatggt agagttcctg gcgatggatg
     1861 tgagaccgct gatcgccgga ggttgctacc gtgcgtcgcc tggttctgat cgtactcatg
     1921 gtctttgcgt tcgtcgccgt gggcacccgg cagttcacga gcagctcagc acccggaggg
     1981 acaccggtct ccgacacact ccgcaccgcg aaccttggcg ccatccaggt cgagggcgcg
     2041 ggtacgaagc tcacgga
//
LOCUS       DASTMD010000661         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1848458, whole genome shotgun sequence.
ACCESSION   DASTMD010000661 DASTMD010000000
VERSION     DASTMD010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848458"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFQ54_09745"
     CDS             <1..187
                     /locus_tag="VFQ54_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEU0165316.1"
                     /translation="AGCSMCLGMNPDQLAPGERCASTSNRNFEGRQGKGGRTHLVSPQ
                     VAAATAIRGAFATPTDL"
     gene            198..815
                     /gene="leuD"
                     /locus_tag="VFQ54_09750"
     CDS             198..815
                     /gene="leuD"
                     /locus_tag="VFQ54_09750"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU0165317.1"
                     /translation="MNPVKKIEGRIVPLDRSDVDTDQIIPAVYLKRVERTGFGEGLFA
                     AWRESDPGFVLNRPEFEGGRVLIAGPNFGTGSSREHAVWALMDYGFDAVISPKFGDIF
                     KNNATKAGLVPVEVDPEFGAELLEAASEQPFLEVVVDLATRTVSVPSLGMTASFPMDD
                     FTQHRLLNGLDDIGLTLQHVDDISAYEATRPSYMPEIVPVGAEAE"
     gene            908..1996
                     /gene="leuB"
                     /locus_tag="VFQ54_09755"
     CDS             908..1996
                     /gene="leuB"
                     /locus_tag="VFQ54_09755"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU0165318.1"
                     /translation="MSENTYRIAVFAGDGVGPEVMAEARRVLTAVGAGIGHTFDFVDL
                     LIGGIAIDAYGTALRDEDVDTARGADAILLGAVGGPKWDNPTASVRPEQGLLKIRKAL
                     GLYANLRPVTVTESLADASPIKADRLLGTDLIVVRELTGGLYFGEPSKQWEENGERVA
                     VDTLIYHEHEIVRVVKLAFELARGRKGKVTSVDKANVLSSSRLWRAIVTEIGKEYGDV
                     ALEHVLVDAMTMKLIQSPSAYDVIVTENMFGDILTDEASVLGGSLGLLPSASIGAADA
                     NGKVLALYEPIHGTAPDIAGQQKANPAGMILSAAMMLRTSFGLEDAAGSVERAVADAI
                     DAGIRTGDLGGTATTSQFSDAVIARLDA"
BASE COUNT          409 a          700 c          816 g          425 t
ORIGIN      
        1 cgcgggatgc tcgatgtgcc tcggcatgaa tcccgatcag ctggcgcccg gcgaacgatg
       61 tgcctcgacg agcaatcgca acttcgaagg ccggcagggc aagggcgggc gcacgcatct
      121 cgtttcaccg caagtcgcag cggcgaccgc gatccggggc gccttcgcga cgccgaccga
      181 tctctaggga ggcacgcatg aatccagtca agaagatcga gggacggatc gttccgctcg
      241 accggagcga cgtcgatacc gatcagatca tccccgccgt ttatctgaag cgcgtcgagc
      301 ggaccggctt cggcgagggg ctgtttgccg cctggcggga gtcggacccc ggattcgtcc
      361 tgaatcgtcc ggagttcgaa ggtggccgcg tcctgatcgc cggtcccaac ttcggcaccg
      421 ggtcgtcgcg cgagcacgcg gtctgggcgc tgatggacta cggcttcgat gccgtgattt
      481 cgcccaagtt cggtgacatc ttcaagaaca atgcgaccaa ggccggcctc gtgccggtgg
      541 aggtcgatcc cgagtttggc gcggagctgc tggaggcggc atccgagcaa ccattcctcg
      601 aggtcgtcgt cgaccttgcg acgcggactg tctccgtgcc cagcctggga atgacggcgt
      661 cgttcccgat ggacgacttc acgcagcatc gcctcctgaa cggactcgac gacatcggac
      721 tcacgctcca gcatgtcgac gacatctccg cgtacgaggc gacgcgaccg agttacatgc
      781 cggagatcgt tcccgtcggc gccgaagccg agtgatcgag aacgtgaatc ttgtctgata
      841 cggaggtcac atcgggcgac acatcgtcgt ctggtgtgac cttctcttgt gggaagggga
      901 acgaatcatg agtgagaaca cgtatcggat tgccgtcttc gctggagacg gagtcgggcc
      961 ggaggtgatg gctgaagccc gtcgcgtgct gaccgcggtt ggcgccggga tcggtcacac
     1021 cttcgacttc gtcgatctgc tgatcggcgg cattgcgatc gatgcctatg ggaccgccct
     1081 gcgggacgag gatgtcgata cggcgcgagg ggcggacgcg attctgcttg gagccgtcgg
     1141 cggcccgaag tgggataacc ccacggcgtc cgttcgtccg gagcagggac tcctcaagat
     1201 ccggaaggcg ctcggactct atgcgaacct gcgcccggtg acggtgacgg aatcacttgc
     1261 cgacgcgtct ccgatcaagg cggatcgctt gctgggaacc gacctcatcg tcgtgcgcga
     1321 gctgacgggt ggtctctatt tcggtgagcc gtcgaagcag tgggaggaga atggcgagcg
     1381 ggtcgccgtc gataccctga tctatcacga gcacgagatc gtccgtgtcg tgaagctggc
     1441 gttcgagctt gcgcgcggtc gcaaggggaa ggtcacgagc gtcgacaagg cgaacgtcct
     1501 gtcgtcatct cgcctgtggc gggcgatcgt gaccgaaatc ggcaaggagt atggagatgt
     1561 cgcgctggaa cacgtactcg tcgatgcgat gacgatgaag ctgatccagt caccgtcggc
     1621 ctacgacgtg atcgtgaccg agaacatgtt cggcgacatc ctgacggacg aggcgtcggt
     1681 tctcggaggc tcgctcggcc tgttgccttc ggccagcatc ggcgccgccg acgcgaacgg
     1741 gaaggtcctc gcgctctacg aaccgatcca cggcaccgcg ccggatattg ctgggcagca
     1801 gaaggcgaat cccgccggca tgattctctc ggcggcgatg atgcttcgca cgtcgttcgg
     1861 tctcgaggat gccgcaggct cggtcgagcg ggcggtcgcg gatgcgatcg acgcggggat
     1921 ccggaccggg gatcttgggg gaacggcaac gacgtcgcag ttctccgacg cggtgatcgc
     1981 gcgtctcgac gcctagtcgc ggctgcgacg acacgtgtct gtagaggtgt tcaaccggac
     2041 gtggcatgcc aaacgaccgc ggtggagtcc ggctgcgccg gatgcgcttc gcgcggggat
     2101 ccttcgacaa gctcaggatg acgaccagga ggggagcggt aggcgcatct tccgaccctt
     2161 gctctagtcg ccccgggacg acagcggtgc gagctgtgac ctgccgcaaa gcggtctagt
     2221 ggggggcgtt cggggcgctg gtgaacggcg tcgtgtggga ggcggtcgcg agcggcagtc
     2281 cgtgaagatc gaccaggggc ggatatgcct cgttcgtccg caacgtcacg atctcgatct
     2341 cgttcacctc
//
LOCUS       DASTMD010000662         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2739342, whole genome shotgun sequence.
ACCESSION   DASTMD010000662 DASTMD010000000
VERSION     DASTMD010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2739342"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFQ54_09760"
     CDS             <1..726
                     /locus_tag="VFQ54_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165319.1"
                     /translation="RNYLRALSRYGRVVTQRNGLLKSFARDRLQPGSINAVTQLAFWD
                     EQLVSTGAYVIASRLCFVDRLSGLMQARSLGIIRDAVIDLSYVSKLDLGSIAENEPHD
                     DVHRLTESVAARFHDQLAVKRPEEFRRGVTSIGPHRDDVMFGMNGRDLAIYGSRGQQR
                     LGVVAIKLAEGDLIAGETGEKPILMLDDVLSELDGAHRDLLLHAVAGNDRQLIVTSTE
                     HPPLDHSSLQYLPMVDVEAGRLR"
     gene            complement(839..909)
                     /locus_tag="VFQ54_09765"
     tRNA            complement(839..909)
                     /locus_tag="VFQ54_09765"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(875..877),aa:Gln,seq:ctg)
     gene            complement(1000..1929)
                     /locus_tag="VFQ54_09770"
     CDS             complement(1000..1929)
                     /locus_tag="VFQ54_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEU0165320.1"
                     /translation="MIRRETRVLKGVALAKRLRAEIGAAGQLLNEQGGRVPVLATLLV
                     GDDGAALAYRSSIQRTFARVQIEHRAVELDGGISPAELIDTVASLNDDPGITGILVLM
                     PLPDHLDPHLVLEHLSPLKDVDGITPVNAGRLHLGLPSLRPSTPAGGLELLDFHGIDL
                     EGSKITVIGRSNVVGRPLATLLTQRHATVTLCHTRTHNLASETANADIVCIAAGSPAL
                     VTGDMVRPGATILDFGVNVLEDGSIVGDADFDSLIGIAGAITPVPGGTGPVTALVLSR
                     NTLAAGFAMIQGSLDHVLLDIPQELLDAYVELT"
BASE COUNT          384 a          644 c          692 g          374 t
ORIGIN      
        1 cgaaactacc tgcgggcact ctcacgctat ggacgggtcg tcacgcagcg caacggcctg
       61 ctgaagagct ttgcgaggga tcggttgcaa cccggctcga tcaacgcggt gacgcaactg
      121 gcgttctggg acgagcaact cgtcagcacg ggagcctacg tcatcgcgag ccggctctgt
      181 ttcgtcgacc gtctctcggg attgatgcag gcgcgatcgc tgggaatcat tcgggacgcg
      241 gtgatcgatc tctcgtacgt ctcgaagctc gatctcggtt cgatcgcgga gaacgagcct
      301 cacgacgacg tgcatcgcct gaccgagagc gtggcggcgc gcttccacga ccagcttgcg
      361 gtcaaacggc ccgaggaatt ccgccgcggc gtcacatcga tcggtccgca ccgcgacgat
      421 gtgatgttcg ggatgaacgg gcgcgatctc gcgatctacg gctcgcgcgg ccagcagcgt
      481 ctcggggtcg tcgcgatcaa gctcgcggag ggcgacctga ttgccggtga aaccggtgag
      541 aaaccgatcc tgatgctgga cgatgttctt tcggaactgg acggagccca ccgggatctg
      601 ctgcttcatg cagtggcagg caacgatcgt cagctgatcg tgacgtcgac cgagcaccca
      661 ccactcgatc attcgtcgtt gcagtatctg ccgatggttg atgtcgaggc cggaaggctg
      721 cgatagcgac gatgtagacc ctgtgcattc tctatgcacg attcccgccg ggatcgggcg
      781 aaacggcaaa gccccgggca atttgcccgg ggctttcttc gtggtcaatt gtgatgggct
      841 ggggtgcagg gattcgaacc ccaactacct gttccagaga caggcgtcct accgttagac
      901 gacaccccat cgcgactgcg cgggcgcaag cttagcatcc gcaaccccgg ggaatcaagg
      961 gcggttgccg tcgatccttc cagattcgtt gtggtgggtt caggtgagct cgacgtacgc
     1021 atcgagcagt tcctgcggga tgtcgaggag gacgtggtcg agcgacccct gaatcatcgc
     1081 aaatccggcg gcgagcgtgt tgcgcgacag gaccagcgcg gtaaccggcc ccgtaccacc
     1141 gggaaccggc gtgatcgcgc cggcgatccc gatcagcgaa tcgaaatccg catcgccgac
     1201 gatcgagccg tcttcgagaa catttacgcc gaagtcgaga atcgtcgcgc cgggtcggac
     1261 catgtccccc gttaccaggg ccgggctgcc cgccgcgatg cagacgatat cggcattcgc
     1321 cgtctccgag gcaaggttgt gcgtccgcgt gtggcagaga gtcaccgtcg cgtgccgttg
     1381 cgtcagaagc gttgccaggg gccgccccac gacgttgctc cttccgatga cggtgatctt
     1441 gctgccctcg agatcgatcc cgtggaagtc caggagttcc agtccgcctg ccggcgtgct
     1501 cggccgcagg gacggcaggc cgagatggag ccgtcccgcg ttgaccggag tgatgccgtc
     1561 gacatccttc aggggtgaca ggtgctcgag aacgaggtgg gggtcaaggt gatccgggag
     1621 cggcatcagg accagaatgc ccgtgatccc gggatcgtca ttgagggatg cgacggtatc
     1681 gatcaactcg gccggcgaga tcccgccgtc gagctcgacc gcgcgatgct cgatctggac
     1741 gcgggcgaat gtgcgctgga tcgacgatcg gtacgcgagc gcggcgccgt catcaccgac
     1801 gaggagtgtc gcgagaacgg gaacgcgtcc tccctgctcg ttcaacagct gaccggcggc
     1861 gccgatctcg gcgcgcaggc gcttggcgag cgcaacgccc ttcagaacgc gggtctctct
     1921 tcgaatcacg tgtgaactcc tggagatggc ggcccgcgct cgcggcggaa cgtgtcacac
     1981 gagacatatc ccagtccgga cagggtgccc aacgcggatt cgagcccgaa tcccgtgcgg
     2041 agtatatcag cgcacgttca aggacatccg tggaaaagcc cgccgcttcg catg
//
LOCUS       DASTMD010000663         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_148359, whole genome shotgun sequence.
ACCESSION   DASTMD010000663 DASTMD010000000
VERSION     DASTMD010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148359"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..634)
                     /gene="aroQ"
                     /locus_tag="VFQ54_09775"
     CDS             complement(155..634)
                     /gene="aroQ"
                     /locus_tag="VFQ54_09775"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEU0165321.1"
                     /translation="MTSISGRKVLVLNGPNLNTLGTREPSIYGTRTLDSIVEELAAHA
                     ASGAPPFTIDPIQSNHEGVLIDFLHERGPAALGIIINPGALSHYSYALRDAISGLKKP
                     TIEVHMSNIHAREQFRHTSVTGEVANGIICGLGARGYLLAADWIRAEVIAKEDANHG"
     gene            complement(645..1625)
                     /locus_tag="VFQ54_09780"
     CDS             complement(645..1625)
                     /locus_tag="VFQ54_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165322.1"
                     /translation="MPRRLITVARFCFVASLAAVICLGSAGSNALAQDSSSPTPSAGN
                     SELTTELPTVDLPTMNSQGFVFEIQSTFDGTPCGTPANSPIYLMNQQNASVDDAKAIA
                     DKLGIKADVTDDGSGTYSAKGNGSLYVTPGLIQYVSGVQSPDGDLPSDQQIIATAREW
                     LRGAGQLPANAGNGSIQTKVASPAQAIVIFQPVTPSPLLSSTPNITVTVGPKNTVLEA
                     AWRWADLKQGDTYQLRPVDQAFGDVTNQIAYLQATLPSDKYPDGTTIKGNVIYAGASV
                     AYASSGVPGEQQYLQPVYVFTGKLTPDGSDTAYPITAYVPALVNSNQPVG"
     gene            complement(1673..>3286)
                     /gene="uvrB"
                     /locus_tag="VFQ54_09785"
     CDS             complement(1673..>3286)
                     /gene="uvrB"
                     /locus_tag="VFQ54_09785"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEU0165323.1"
                     /translation="TLIVASVSCIFGLGAPEEYANTVVNLKRGMQVRRDRVLRQLIDI
                     QYDRNDMTLTRGTFRVRGDTVEIYPAYEEIAVRVEFFGDEVDRIVDVDPLTGEILAVR
                     EEIGIYPAKHFVTSEDKLKVAIKDIEQELEERLQQLNDNGKILEAARLRQRTMYDLEM
                     MQEAGYCAGIENYSMHLSRRKPGDQPSTLLDYFPDDYLLLVDESHMTLPQVRGMFGGD
                     RSRKDVLVEHGFRLPSARDNRPLTFPEFERMIKQAVFISATPGPYEYEHTSRIVEQVI
                     RPTGLIDPEISVRPTKGQIDDLLAEINQRVARNERVLVTTLTKRMAEDLADYLKEMGV
                     RTHYLHSEVDTFERIEILRDLRLGIHDVVVGINLLREGLDLPEVSLVAILDADKEGYL
                     RSEGSLVQTIGRAARHVDGHVIMYADKMTRSMKAAIDETYRRRGIQTAYNEAHGIQPR
                     GIVKEIKDLTDRVRAVAEETAEYRASGTDGHPGMIAEIPKDELARMIKDLESQMKTAA
                     RDLEFEKAALLRDQIVELRRIIDVDPISA"
BASE COUNT          556 a          982 c         1086 g          662 t
ORIGIN      
        1 gccgttcgca tcgatcgcca ccacgccgga cgactcgtct ggggcgtgat cctcggctgt
       61 gtagccggtc aggtagcggc gattcgttcc gctgctgatg acggcggcgt ccagctgttg
      121 ctccgcaagg gcgacgcgta ggcgatcgag acggctaccc atgattggcg tcctctttgg
      181 cgatgacttc ggcccggatc cagtcggcgg cgaggagata gccgcgcgct ccgagcccgc
      241 agatgatccc gttcgccact tctccagtaa ctgaggtgtg gcggaattgc tcccgggcat
      301 ggatgttcga catatgtact tcgatcgtcg gcttcttgag cccggagatc gcgtcccgca
      361 atgcgtagct gtaatgcgac agtgcgccgg gattgatgat gatgccgagc gcggcgggac
      421 cgcgctcgtg caggaagtcg atgaggacgc cctcgtggtt cgactggatc gggtcgatcg
      481 tgaacggagg ggcgccgctc gccgcatgcg cggcgagctc ctccacgatc gagtcgaggg
      541 ttcgcgttcc gtagatcgac ggctcccgcg tgcccagggt gttcaggttc ggcccgttca
      601 ggacgagcac cttgcgtccg gaaatcgacg tcattggttc tggattaccc caccggttga
      661 ttgctgttga ccagcgccgg aacgtaggcg gtgatcgggt aggccgtatc cgacccgtcg
      721 ggtgtcagct tgccggtaaa gacatagacc ggctgcagat actgctgctc gcccggcaca
      781 ccggaggaag catacgcaac gctggcaccg gcgtaaatca catttccctt gatcgtcgtg
      841 ccgtcgggat acttgtccga tgggagcgtc gcctgcaggt aggcgatctg gttggtgacg
      901 tcgccgaatg cctggtcgac cggccgcagc tggtaggtat cgccctgctt gagatcagcc
      961 cagcgccacg ccgcttcgag cacggtgttc ttggggccga cggtcaccgt gatgttcggc
     1021 gtcgaggaaa gcaggggact tggggtcacc ggctgaaaga tgacgatcgc ctgggccggt
     1081 gacgccacct tcgtctggat cgacccgttg ccggcgtttg ccgggagttg cccggccccg
     1141 cgcagccact cacgcgcggt ggcgatgatc tgctgatcgg acggcaggtc gccatctggc
     1201 gactggacgc cggagacgta ctggatgagg ccgggcgtca cgtagagtga cccatttccc
     1261 ttggcggagt aggttccgct gccgtcgtcg gtgacatcgg ccttgatgcc gagcttgtcg
     1321 gcgatcgcct tggcgtcatc gacggacgcg ttctgctggt tcatgagata gatcggcgaa
     1381 ttcgccgggg tgccgcatgg cgtgccgtcg aacgtcgact gaatctcgaa gacgaacccc
     1441 tgggagttca tcgtcggcag gtcgactgtt ggcaattcgg tggtgagctc ggaattgccg
     1501 gcggatggtg tcgggctcga cgaatcctgg gcgagcgcgt tcgatccggc gctgcccagg
     1561 cagatgacgg cggcaaggga cgcaacgaaa cagaaccgcg cgactgtgat gagtcgacgt
     1621 ggcatgaggg agatcctttc gcgaggagga ccctgaggca gttgctgtcg cgtcaggcgc
     1681 tgatgggatc gacgtcgata atgcgccgca attcaacgat ctgatcgcgc aggagcgccg
     1741 ccttctcgaa ctcgagatca cgggcggcag tcttcatctg cgattcaagg tccttgatca
     1801 tgcgcgcgag ctcgtccttc ggaatctcgg caatcatgcc gggatgtcca tcggtacccg
     1861 aagcacggta ctcggcagtc tcttcggcga cggcacggac gcggtcggtg aggtccttga
     1921 tttccttgac gataccgcgt ggctgaattc cgtgcgcctc gttatacgct gtctgaatcc
     1981 cgcggcgacg gtacgtttca tcgatcgccg ctttcatcga ccgggtcatc ttatcagcgt
     2041 acatgatgac atgaccatcg acgtgccgcg ccgcacgacc gatcgtctgc acgagcgagc
     2101 cctccgagcg gaggtatccc tccttgtccg catcgagaat tgcgaccagc gagacctcgg
     2161 gaagatcgag tccttcgcga agcagattga tgccgacgac gacatcgtgg atgccaagcc
     2221 ggagatcgcg gaggatctcg atgcgctcga aggtatcgac ctcgctgtgc aggtaatggg
     2281 tgcgaacgcc catctccttg agatagtccg cgaggtcctc cgccatccgc ttcgtcagcg
     2341 tcgtgacgag cacgcgctcg ttgcgggcga cgcgctggtt gatctccgcg agcagatcgt
     2401 cgatctgacc ctttgtcggg cggaccgaga tctccgggtc gatcagaccc gtcgggcgaa
     2461 tcacctgctc gacgatgcgc gaggtgtgct cgtactcata cggtcccggc gtcgccgaaa
     2521 tgaagacggc ttgcttgatc atccgttcga actccgggaa ggtcaggggc cggttgtcgc
     2581 gggccgaggg gagccggaat ccgtgctcga cgagcacgtc cttccgcgag cgatcgccgc
     2641 cgaacatgcc gcgtacctgt ggcagcgtca tgtgcgactc gtcgacgagg aggagatagt
     2701 cgtccgggaa gtaatccagg agggttgacg gctggtcgcc cggcttccgg cggctgagat
     2761 gcatcgagta attctcgatc ccggcgcagt agccggcttc ctgcatcatt tcgaggtcgt
     2821 acatcgtgcg ttgcctgagc cgcgccgcct cgaggatctt gccattgtcg ttgagttgct
     2881 gcaggcgctc ctcgagctcc tgctcgatat ccttgatcgc gaccttgagc ttgtcctcgc
     2941 tcgtcacgaa gtgcttcgcc gggtagattc cgatctcctc acggaccgcc aggatctcgc
     3001 cggtgagcgg atcgacatcg acgatccggt cgacctcgtc gccgaagaac tcgacgcgga
     3061 cggcgatctc ctcgtacgcc gggtagatct cgaccgtgtc cccgcgcacg cgaaaggtgc
     3121 cgcgggtcag cgtcatgtcg ttgcgatcgt actggatgtc gatgagttgg cggagcacac
     3181 gatcgcgccg aacctgcatg ccgcgcttca ggttgacgac cgtattcgcg tattcctctg
     3241 gcgcaccgag accgaagatg caggagaccg aggcgacgat caacgt
//
LOCUS       DASTMD010000664         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_865251, whole genome shotgun sequence.
ACCESSION   DASTMD010000664 DASTMD010000000
VERSION     DASTMD010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865251"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFQ54_09790"
     CDS             complement(<1..312)
                     /locus_tag="VFQ54_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165324.1"
                     /translation="MVARTLRVMSAVILVWVFAASSSSAAPLAQVDEMTPTAPPPVTV
                     QAIDAGCLSGTSVAGIITIANPDGATAIGLSLQYPYHFSQHVTLWLGVANSTVEADSG
                     TE"
     gene            533..1501
                     /locus_tag="VFQ54_09795"
     CDS             533..1501
                     /locus_tag="VFQ54_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEU0165325.1"
                     /translation="MSEKITTILVPAADTYVTEGGGNIEDPSRILATIPNVRLLTFEP
                     NTPLPGGASDVEILVTGGSVHPPMIAGFPGSLPNLRMIQTLSAGVNNWLGHVPDGVIL
                     CNARGAHGGATSEWAATALLTIFRDFPRYILAQPRHEWARIRSEGLHDKRVLIVGAGD
                     LANQLKRKLVAFDAIVTLVGRTAREGVHSFDDLPGLLPRQDAVVLMVPQTTTTTHLVD
                     AAFLARMEDHAVLINAARGPIVDTNALLAELTSGRLRAGLDVTNPEPLPADHPLWDLP
                     NVLITPHVGGATDGGDTRSWTVAREQVTQFLAGQRPSNAFAPEDLG"
     gene            1553..>2252
                     /locus_tag="VFQ54_09800"
     CDS             1553..>2252
                     /locus_tag="VFQ54_09800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0165326.1"
                     /translation="MSEVPSLPVAVITGAGSGIGRAVARTLLDHGYRVALAGRREDAL
                     IETIDGRANALAVPTDITDPAQVDALFTKIVAAWGRVDLLMNNAGMSGGGAIDEVSFE
                     AWSQTVALNLTGSMLCAAAAFKVMRDQTPRGGRIINNGSISAHHPRPNSVAYTATKHA
                     ISGLTKSIALDGRKYDITCGQIDIGNAATEMTARMADGVLQANGELAVEPTFDPYEVG
                     RAILYMASLPASANI"
BASE COUNT          404 a          788 c          702 g          358 t
ORIGIN      
        1 ctcggtgccg gagtcggcct cgacggtact gttggcaacg ccgagccaga gcgtcacatg
       61 ctggctgaaa tggtaggggt actggagcga caacccgatc gcggtcgccc catccggatt
      121 cgcgatcgtg atgattccgg caacgctggt tcccgagagg caacccgcat cgatcgcctg
      181 cacggtgacg ggaggtggcg cggttggcgt catctcatcc acctgcgcga gcggggcggc
      241 gctgctggaa ctcgcggcaa aaacccagac aaggatgacg gcgctcatca cccgaagcgt
      301 gcgtgcgacc atgtatctga cccttgatat gaacggaccg gtccccaatc ccattcctta
      361 tacacaaggc gttcccgata cgcaatcgtg cgagaacccg aatgatgtct tcccgagcac
      421 actccccagg ttgctgtgcc aggcgaaaac ggttcgccgg gcgtgagatc cgttgctttc
      481 tggcacactg gcagcaacat gtgaaacgcc atcgccggag gagtatccgt tcatgtccga
      541 gaagatcacg accatcctcg ttcccgccgc cgatacgtat gtcacggagg gcggaggcaa
      601 tatcgaggat ccatcgcgca tcctcgcaac gatcccgaac gtccgcctgc tcaccttcga
      661 gccgaacaca ccccttcccg gcggcgcgtc cgatgtcgag atcctcgtga ccgggggcag
      721 cgtccatccg ccgatgatcg ccggattccc cggcagcctc ccgaacctga ggatgatcca
      781 gaccctcagc gccggcgtga acaactggct cggacacgtc ccggacggcg tgattctctg
      841 caatgcgcgc ggagcccatg gcggcgcgac ctcggaatgg gcggcaacgg cgctcctgac
      901 gatcttccgc gacttcccgc ggtacatcct tgcccagccg cggcacgagt gggcgcgcat
      961 ccgcagcgag ggcctccacg acaagcgtgt cctgatcgtc ggcgccggcg atctcgcgaa
     1021 ccagctcaag cggaagctcg tcgccttcga tgccatcgtc acgctcgtcg gacgcaccgc
     1081 gcgggagggc gtccacagct tcgacgacct gccggggttg ctcccgcggc aggacgcggt
     1141 cgtcctgatg gtgccgcaga cgacgaccac tacccacctc gtcgatgccg ccttcctcgc
     1201 acggatggag gaccacgccg tactgatcaa cgcggcgcgc ggcccgatcg tcgatacgaa
     1261 cgcgctgctc gccgagctga cctcggggcg cctgcgagcc gggctcgacg tcaccaatcc
     1321 cgagccgctt cccgccgatc atccgctttg ggatctcccg aacgtcctga tcacgccgca
     1381 tgtcggtggt gcgacggatg gtggcgatac gcgatcctgg accgtggcgc gcgaacaggt
     1441 gacgcaattc ctggccggac agcgaccgtc gaatgccttc gcgccggaag atctcgggtg
     1501 agatcgcggc atcaaccgat ccctatcgcc atctcaccga aaggtccgat ccatgagtga
     1561 agtcccgtcc ctgccggtcg ccgtcatcac cggcgccgga tccggtatcg gccgcgccgt
     1621 cgcccgcacg ctgctcgacc acggctaccg cgtcgccctc gccggccgcc gcgaggacgc
     1681 gctgatcgag accatcgacg gacgcgcgaa tgcccttgcg gtcccgaccg acatcaccga
     1741 ccccgcgcag gtcgacgcgc tcttcacgaa gatcgtcgcc gcgtgggggc gcgtcgatct
     1801 cctgatgaac aacgccggga tgtccggtgg cggggcgatc gacgaggtca gcttcgaggc
     1861 ctggagccag accgtcgccc tcaacctgac gggttcgatg ctctgcgccg ccgccgcgtt
     1921 caaggtgatg cgggaccaga cgccacgcgg cggacgcatc atcaacaacg ggtcgatctc
     1981 ggcgcatcac ccgcgcccga attccgtcgc ctacaccgcg acgaagcacg cgatcagcgg
     2041 gctgacgaaa tcgatcgcgc tcgacggccg caagtacgac atcacctgtg gccagatcga
     2101 catcggcaac gcggcgacgg agatgacggc gcggatggct gacggcgtgc tccaggcgaa
     2161 cggcgagctc gcggtcgagc cgaccttcga tccctacgag gttggtcgcg caatcctcta
     2221 tatggcgagc ctgcccgcga gcgcgaacat cg
//
LOCUS       DASTMD010000665         5874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1221187, whole genome shotgun sequence.
ACCESSION   DASTMD010000665 DASTMD010000000
VERSION     DASTMD010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221187"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFQ54_09805"
     CDS             complement(<1..417)
                     /locus_tag="VFQ54_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165327.1"
                     /translation="MLLADWGEMQDRLRLPATYVFKHVNSRPHLRTSFFEAVARLPLT
                     FTVVAADKLDWSRHYLDESTGLIRIIDQVDALIEGCPGEYVEDQILVIDGHHGDHGLV
                     RSMTQRLRQSARSRGHRSFKKISAVPDHRKDSLLIQA"
     gene            complement(638..1708)
                     /locus_tag="VFQ54_09810"
                     /pseudo
     CDS             complement(638..1708)
                     /locus_tag="VFQ54_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
     gene            complement(1830..4958)
                     /locus_tag="VFQ54_09815"
     CDS             complement(1830..4958)
                     /locus_tag="VFQ54_09815"
                     /inference="COORDINATES: protein motif:HMM:NF005544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEU0165328.1"
                     /translation="MVLDEQRTLSDSAADAVNADNLIVRKRDGREVSFDHARISHAIE
                     MAFRAEMGIPYPDPIASTFAERIRAVTDAVVASLPAVADHGNAATVEEIQDEVERQLM
                     ATGAYSVARRYILYREARARARDEDQLRLRDHSGQEMLVNRAVLRGWIADAATGLGPD
                     IQTKDIEDDVLATVRHGMALTELDQAIVMAGRSRIEQRPIYSSFAARALLRSIYSDVV
                     GRRVNAADADFFYAETFIAKIHEGIENELLTPELATFDLERLGAALRPERDEQFLYLG
                     LQTLFDRYLIHNRGRRIELPQAFWMRVSMGLALGEVEDQRNDRAIEFYTALSTFRVCS
                     SSPTLFNAGTLHAQLSSCYLTTVSDDLDHIFKSVRDNALLSKWSGGLGNDWTNVRALG
                     SRIKGTNGESQGVIPFLKVVNDAAVAVNQGGKRKGAVATYLEIWHSDIEEYLELRKNT
                     GDERRRTHDMNTATWIPDLFMQRVEEDGEWTLFSPNEVPDLHDLYGKAFKARYEEYEA
                     LHAQGAFPRARTIRALDLWRRLLSATFETGHPWITFKDPANVRSPQDHAGVVHNSNLC
                     TEILLNTSQDEVAVCNLASINLLTHMKDGVLDAEMIGETVKVAIRMLDNVIDINYYPI
                     PEAANSNQKHRPIGLGIMGFQDALWKMGLSYASDEAMEFADRSMELISWHAIYSSSQL
                     AAERGAYPSFKGSKWDRNLLPIDTIALLAKERGEAIDVDMSISLDWEPVRESIRQNGM
                     RNSNTMAIAPTATISNIQGVAQSIEPIFSNLFVKSNLSGEFTIANESLVAELDAQGIW
                     DADMLEEIKYWDGSIALIERIPLDIRMHYPTAFEVEPAWLIEAASRRQKWIDMGQSLN
                     LYISEPSGKKLHDMYFLAWKKGLKTTYYLRSRGATQAEKSTLDVNRFGIQPRWMKSRS
                     ASSHVSITRTTPAAPTSAPQARLTEAAAPDPSHTTLVPKAVVSVPAPAAAIAPRLAPA
                     PAQVRTPLVPKAAPMASSEPAVPVMRKPDVSVPFDLTPPVAADLPVIKASVPIASNGL
                     SVEEEFECEACQ"
BASE COUNT         1095 a         1827 c         1799 g         1153 t
ORIGIN      
        1 cgcctgaatc agcaacgaat ccttgcgatg atctggaact gctgagatct tcttgaagct
       61 acgatgccca cgcgaacgag cgctctgccg aagccgctgc gtcatcgagc ggaccagccc
      121 gtgatctccg tgatgtccgt caatcaccag gatctgatcc tcgacgtatt ctccaggaca
      181 cccctcgatc agtgcatcga cttgatcgat aatccggatt aggcccgtgg attcgtccag
      241 ataatgcctc gaccaatcca atttgtccgc cgccacaact gtaaaagtga gaggcaatcg
      301 agccaccgcc tcgaagaagc tcgtacgcag atggggtcga gaattcacgt gcttgaatac
      361 atacgtggcg ggaagccgaa gacgatcctg catctcgccc caatccgcca gtagcatacc
      421 gtcaacgtat tccttggaag catggcacgc cgcggtcaca atccatgcgt tgggtttgtc
      481 cgatcttgtc ggatcacccg gatcaccagc ccaatccagt ccggtaacta cacgtaccgg
      541 cacatgaacc cctcattcaa cggcgctccg atcaatacct catcaaaggg cggggccgaa
      601 tcggccccgc cctctctcgc actgctggtg attccgccta ctcccaggtg agggtggagc
      661 ccttctggta gtccgtgacg cggctctcga agaagttctt ttccttcccg aggtcgatcg
      721 tctcgctcat ccacgggaac gggttcttgt ccatgtactg ccgcggcagc ccgatccgct
      781 ccagacgacg atccgcgatg tggcggacgt actgctcgaa gagttcggcg ttgagaccga
      841 gcacaccgtt cgggaggcaa tcccgcgcgt acgcctcttc cagctccacg gcctgacgga
      901 tcttggcgac catcttcgcg cggaactcct ccgtccacag ctccgggttc tcgtggatga
      961 tcccgttgat caggtcgaca ccgaagttga gatggatcgt ctcgtcgcgc aggatgtact
     1021 ggaactgctc gccgatcccc ttcatcttgt tctggcggtg aaaggagagg atcatcgcaa
     1081 agcccgcata gaagaagatg ccttccatga tgacgtagaa cccgatcagg ttctcgatga
     1141 acttctgcgc gccttcgaac gtcgccgtgc tgaattacgg atcgacgata tcggccgtca
     1201 gctccatctc gaactcatcc ttggcgcgga tagccgggat gtcgtgatac atgccgaaga
     1261 cttcctgctc gtcgagaccc aggctctcca ccacgtagtg gaaggtgtgg gtgtggatcg
     1321 cctcctcgaa ggcctggcgg aggaggtact ggcggcactc ggcgttggtg acgtgcttga
     1381 agatcgccag cgtcaggttg ttggcgacaa ggctctccgc cgtcgcgaag aaaccgaggt
     1441 tgcgcatgat gacgaggcgc tcgtccggcg agagctggtt gctccgccag agcgcgatgt
     1501 cggcgcccat cggcacctcg gtcggcatcc agtggttggc gcaaccgttg agatagtgct
     1561 cccacgccca ctcgtacttg agtggcatca actggttgac gtcaaccgtg ctgttgttga
     1621 tcagacgctt gaaatcgggg ttgcgcaccg cctcgcgtgg tgcgacttcg actccgagtt
     1681 gtccatgtgt ctgggtcatg cgtctcattc ccctcgggat gaaggggcaa ccccgccggc
     1741 ggggttgccc ttgctatcgc gccctcggag gttctgtcgg agacccgggg gctatcgtcg
     1801 aattcggcgg ggcgattctt gccagtcgct tactggcagg cctcgcactc gaactcttcc
     1861 tcgacggaga gcccgttgct ggcaatcggc acggacgcct tgatcaccgg caggtcggcc
     1921 gcgacgggcg gcgtcagatc gaacggaacc gaaacatccg gcttccgcat caccggcacg
     1981 gccggttcgc tggaggccat cggcgctgcc ttcggcacca gcggcgtgcg cacctgggcg
     2041 ggtgcgggcg ccaggcgcgg cgcaatcgct gccgccggag ccggaacgct caccaccgcc
     2101 ttcggcacga gcgtcgtgtg cgacgggtcc ggcgcggccg cctcggtcag ccgcgcctgc
     2161 ggcgcggagg tcggtgccgc cggcgtcgtg cgcgtgatcg agacatggct cgacgcgctg
     2221 cgcgacttca tccagcgcgg ctggatgccg aagcggttga cgtccagcgt cgacttttcg
     2281 gcctgcgtcg cgccgcggga ccggaggtag taggtcgtct tcagaccctt cttccaggcc
     2341 aggaagtaca tgtcatgcag cttcttgccg ctcggctccg agatgtagag gttgagcgac
     2401 tgccccatgt cgatccactt ctggcgacgg gaggcggcct cgatcaacca cgccggctcg
     2461 acctcgaagg cggtcggata gtgcatgcgg atatcgaggg ggatgcgctc gatcagggcg
     2521 atcgatccgt cccagtactt gatctcctcc agcatgtccg catcccagat accctgggcg
     2581 tcgagctcgg ccaccagcga ttcgttggcg atcgtgaact cgccggagag gttgctcttg
     2641 acgaagaggt tgctgaagat cggctcgatc gactgcgcga caccctggat gttggagatc
     2701 gtcgccgtcg gcgcgatcgc catcgtgttg ctgttgcgca tgccattctg gcgaatcgac
     2761 tcgcgcaccg gctcccagtc gagggagatg ctcatgtcga catcgatcgc ctcaccgcgc
     2821 tccttggcga gcagcgcgat ggtgtcgatc ggcagcaggt tgcggtccca cttcgacccc
     2881 ttgaaggacg gatacgcgcc gcgctcggcg gcgagctgcg aggacgagta gatcgcgtgc
     2941 caggagatca gctccatcga gcgatcggcg aactccatcg cctcgtcgga ggcgtaggag
     3001 agacccatct tccacagcgc atcctggaag cccatgatgc cgagaccgat cgggcggtgc
     3061 ttctggttgg aattcgccgc ctccgggatc gggtagtagt tgatgtcgat cacgttgtcg
     3121 agcatccgga tcgcgacctt gacggtctcg ccgatcatct cggcatcgag gacgccatcc
     3181 ttcatgtgcg tcagcaggtt gatcgaagcg aggttgcaga ccgcgacttc atcctggctg
     3241 gtgttgagca ggatctcggt gcagagattc gagttatgca cgacaccggc gtgatcctgc
     3301 gggctgcgga cattggccgg gtccttgaag gtgatccacg gatgtccggt ctcgaaggtc
     3361 gcgctcagca ggcggcgcca gaggtcgagt gcgcggatcg tgcgcgctcg cgggaaggct
     3421 ccctgcgcat gcagcgcctc gtactcctcg tagcgtgcct tgaaggcctt gccgtagaga
     3481 tcgtgcaggt cgggaacctc gttcgggctg aagagcgtcc actcgccatc ctcttcgacg
     3541 cgctgcatga agaggtccgg gatccaggtc gccgtgttca tgtcgtgcgt gcggcggcgc
     3601 tcgtcaccgg tgttcttgcg aagctcaagg tactcctcga tatcgctgtg ccagatctcg
     3661 agataggtcg cgaccgcgcc cttgcgcttg ccgccctggt tcaccgcgac ggcggcgtca
     3721 ttgacgacct tgaggaaggg gatcacgccc tggctctcgc cattcgtgcc cttgatccgg
     3781 ctgccgagcg cgcggacatt cgtccagtcg ttgccgagac caccactcca cttcgaaagg
     3841 agcgcgttgt cgcgcacgct cttgaagatg tgatcgagat cgtcggagac ggtcgtcagg
     3901 tagcaggacg agagctgtgc gtgcaacgtg ccggcgttga agagcgtcgg gctggaggag
     3961 cagacacgga aggtcgagag cgcggtatag aactcgatcg cgcgatcgtt ccgctggtcc
     4021 tcgacttccc cgagcgcgag ccccatcgag acgcgcatcc agaacgcctg cggcagttcg
     4081 atgcgacgac cgcgattgtg gatcaggtag cgatcgaaga gcgtctgcag gcccagatag
     4141 aggaactgct cgtcgcgctc cggccgaaga gcggcgccaa ggcgctcgag atcgaaggtg
     4201 gcgagttccg gcgtgagcag ctcgttttcg attccctcgt ggatcttggc gatgaaggtc
     4261 tccgcataga agaaatcggc gtcggcggcg ttgacgcggc gtccgacgac atcgctgtag
     4321 atgctgcgga gcagggcgcg ggcggcgaac gacgagtaga tcgggcgctg ctcgatgcgg
     4381 gaacgacccg ccatcacgat cgcctggtcg agctcggtaa gcgccatgcc atggcgaacc
     4441 gtcgcgagga cgtcatcctc tatgtccttg gtctggatgt ccggaccaag cccggtcgcg
     4501 gcgtcggcga tccagccgcg gagcacggcg cgattgacca gcatctcctg gccactgtga
     4561 tcgcgcaggc ggagctggtc ttcgtcacga gcgcgggctc gggcctcgcg gtagaggatg
     4621 tagcggcgag cgacggaata ggcgccggtc gccatcaact gacgctcgac ctcgtcctgg
     4681 atctcttcga ccgtcgcggc gttgccgtga tcggcgaccg ccggaaggga ggcgacgacg
     4741 gcatcggtca ccgcccgaat gcgttcagcg aaggtggagg caatgggatc ggggtaggga
     4801 atgcccatct cggcgcggaa cgccatctcg atcgcatgag aaatacgcgc gtgatcgaac
     4861 gagacttcac gaccgtcgcg cttgcgaacg atgaggttat ccgcgttgac agcatccgcc
     4921 gccgaatccg atagagttcg ctgttcgtcc agtaccatgt gacccttctc caacctctgg
     4981 cgttaccggc agggaccgat ccgcctcccc ttctgcgggg ttcaatggag tgagcaacgc
     5041 tctccggaag gaaaaccacg aaggcgatcc gttgtgggga tcgttctcgt catgtcctcc
     5101 gcaagtaccg tactcaactc ccgctcgcga gcttgatgga cctctcaaag gcgaacatcc
     5161 gaagccggat cgcccgattg ttgcctttat ccgtacaccc tcgccggtga catcaccgct
     5221 ccatccgggg catgtacgag tcacgaaaat gccgctctga aatatgaacg gcctgctggt
     5281 gctggtacta gatattgtac caccttaccg gcaggaaccc atcatatggt gccagaacgt
     5341 catagtcaag atcatttgcg ggacttctcg agaccgttcc ggaactcgtc ttcccccatt
     5401 cgtacgatgg tccagccact atctcacatg tacgtacatc gctgtcaaga cgtcgaatcc
     5461 acgtaattca agactattaa gaatgtcgtc aatatacgct cccgataccg ccgaatcccg
     5521 cgccggaaca accccggatc attatcaacg cctctccaca cgtgccacaa aacgcgccag
     5581 aatccgccaa aaaaccgctc aaaagttcga aacgcacgga cacaggcgac gcaatcgcca
     5641 caccgccgtc gtccccttta tcgccagccc cgcgccgatc gcatccgcgc tgatcccggt
     5701 cgtcaacaac gggtttcccg ggtgtccctt taccttttgt caagtaatgc gggaatgcca
     5761 acgattcgat ctcatgcttg tgccgggcgc cccgcttaac aatcagaaga taaaacagga
     5821 cattccgaac aaacgatcta ggcaccggtg acgcctcgac ccgcggtgaa ggtc
//
LOCUS       DASTMD010000666         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2919516, whole genome shotgun sequence.
ACCESSION   DASTMD010000666 DASTMD010000000
VERSION     DASTMD010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2919516"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFQ54_09820"
     CDS             complement(<1..285)
                     /locus_tag="VFQ54_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0165329.1"
                     /translation="MSLAVIDTSEMDRAQLADFLRRRREALQPEDVGLAKGRRRRTVG
                     LRREEAAALCGMSVDYLTRLEQERGPQPSEQMLASISSGLHLSLEERDHLF"
     gene            complement(324..1202)
                     /locus_tag="VFQ54_09825"
     CDS             complement(324..1202)
                     /locus_tag="VFQ54_09825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU0165330.1"
                     /translation="MKFGIHFTNFNLPGGAESFSTTLRDTAIAAESAGADRFTLMDHW
                     FQMEQFGRSEDPMLEGYTSLGFLAGQTEKMTLGLLVTGITYRQPGLLAKIVTTLDVLS
                     KGRAMLGIGAAWYEREHLGLGVPFPPISERFERLEETLQICQQMWSDNDGPYDGKYYH
                     LAETICSPLPVRRPHPPILIGGGGEKKTLRLVARYADASNLFAFGETTDEIRDKLDVL
                     ARHCDTEGRDYAAIEKTILGGGDPFTDPDGFLRMMEQYAALGVELVEIVPRGSDPVAM
                     VAGLASYVAPRLAEIG"
     gene            1406..1906
                     /locus_tag="VFQ54_09830"
     CDS             1406..1906
                     /locus_tag="VFQ54_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0165331.1"
                     /translation="MQHSASRTSYQIREAAAGSPIADGLLRRYYADLVDLLPGGFSAP
                     DAIAADPGELTPPRGIFLVVELDDLPAGCAAIRDLGAGAFEIKHMWLDAACRGLGIGT
                     ALVEHLEAFAIAHGATEVRLDCSSHLDAAIRMYERAGYRRVPAYNANPYADVWFAKSL
                     GVVAPS"
BASE COUNT          358 a          645 c          676 g          381 t
ORIGIN      
        1 gaagaggtgg tcgcgctcct cgagcgagag atgaagcccg ctcgagatcg aggcgagcat
       61 ctgctcggac ggctgtggtc cgcgttcctg ctcgagtcgc gtcaggtagt cgaccgacat
      121 gccacagagc gctgccgcct cctcgcggcg aaggccaacc gtgcgacggc gccgtccttt
      181 cgcgagtcca acatcttcgg gttgaagcgc ctcccggcgc cggcgcagaa agtctgccag
      241 ttgagctcgg tccatctccg aagtatcgat aaccgcgaga ctcattgcca ctcccttccg
      301 cgccggccac cgtgtgccac gagctagccg atttcggcga gacgcggtgc gacgtagctc
      361 gcaagacccg cgaccatcgc gaccggatcg gaacctcgcg gcacaatctc gacaagttcg
      421 actccgagcg ccgcgtactg ttccatcatt cgcaggaacc cgtcgggatc ggtgaagggg
      481 tcgccgccgc cgaggatcgt cttctcgatc gcggcgtagt cacgtccctc ggtgtcgcaa
      541 tgccgcgcaa gcacatcgag cttgtcgcga atctcgtcgg tcgtctcgcc gaacgcgaag
      601 agattgctgg cgtccgcgta cctggcgacg agacgaaggg tcttcttttc ccccccgccc
      661 ccgatcagga tcggcggatg cggccgccga acgggcagcg gcgagcagat cgtctcggcc
      721 agatggtaat acttgccatc gtacggtcca tcgttatcgc tccacatctg ctggcagatc
      781 tgcaacgtct cctcgagccg ttcgaagcgc tcggagatcg gtgggaacgg tacgccgaga
      841 ccgaggtgct cgcgctcgta ccaggcagcg ccgatcccga gcatcgcgcg ccccttcgac
      901 agtacatcca gggtcgtgac gatcttcgcg agcagtccgg gttggcggta ggtgatgccg
      961 gtgacgagga gaccgagcgt catcttctcg gtctgcccag cgaggaagcc aagggacgtg
     1021 tagccctcga gcatgggatc ctcggatcgg ccgaactgct ccatctggaa ccagtggtcc
     1081 atcagggtga accgatcagc gccggcgctt tccgcggcaa tcgccgtgtc ccgcagcgtt
     1141 gtggagaagg attctgcgcc gccgggaagg ttgaagttgg tgaagtgaat gccgaatttc
     1201 atcgtgatgc tcctgggttg accgggtccg gtcccgccgc gtatcgtggc gccgtgtgcg
     1261 ccgagatgaa ctgtagattc gccctgccgc gccgggaaga gacggcggat cccaggattg
     1321 gcgatcccac catcatggga tccgatgcaa tgttcctggg tgatgtccga cgatgaacca
     1381 cgatcgacac cgggagcagg agcgcgtgca acattcagcg agtcggacgt cctatcagat
     1441 tcgcgaggcg gcagcgggaa gccccattgc cgatggtctg ctgcggcgct actacgcgga
     1501 tctcgtcgac ctgttgccgg gcgggttctc ggcgcccgat gccatcgcgg cggatccggg
     1561 agagctgaca ccaccccgcg ggatcttcct cgttgtcgag ttggatgatc tgccggcggg
     1621 atgcgcggct atccgggatc tgggcgccgg cgccttcgag atcaagcaca tgtggctcga
     1681 tgccgcctgc cgtgggctgg ggatcggaac ggcactcgtc gagcatctcg aagcgttcgc
     1741 gatcgcgcat ggagcgacgg aggtccggct tgattgcagc agccacctcg acgcggcgat
     1801 ccggatgtac gagcgggcgg gatatcgccg cgttcccgcc tacaacgcga atccctacgc
     1861 cgacgtctgg ttcgcgaaat ccctgggcgt ggttgctcct tcatagtgaa agatttccac
     1921 ctcgatcccc ggcccgggtg ttgatcggtg gaacgaatgc ggggatactg acgcatcaca
     1981 cgccgccacg agccgcgaag gtcggaaggt gcttccatgg atcaggtcgc tttgttggat
     2041 gaactgcagc ggatcgtcgg
//
LOCUS       DASTMD010000667         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_148911, whole genome shotgun sequence.
ACCESSION   DASTMD010000667 DASTMD010000000
VERSION     DASTMD010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148911"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VFQ54_09835"
     CDS             <1..728
                     /locus_tag="VFQ54_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0165332.1"
                     /translation="ERRAEDARRLLERGYTAIKLRLHDETMRQDIAQVEAVRDAVGDR
                     MTILVDANQAQEPGTPGAERSIFWGYNRALATARELQQLGVTWLEEPLPRYDFDNQRR
                     LAAATDLPIAGGENNIGLHEFRTLIDTGCYDVLQPDALVCGGISMLRKIAAYAEMHGK
                     PVAPHHGGNGLGVATHLHLSASLPNSPWVELLQDPPAMEASEFQGILATPLVPGKDGI
                     VPLPEAPGLGVELAEHVLAALPG"
     gene            complement(758..2242)
                     /locus_tag="VFQ54_09840"
     CDS             complement(758..2242)
                     /locus_tag="VFQ54_09840"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0165333.1"
                     /translation="MTLSARLKIDLERRIAPVDPRIYGGFIEHLGRCIYGGVFEEGSP
                     LSDARGFRTDVMAEVAGLQMPILRWPGGNFVSGYHWTDGIGPKDERPRRIELAWRQEE
                     SNRFGTDEFIEYCRAIGAEPYICVNMGTGTVEEAQNWVEYCNGTGNTYWANQRRANGH
                     DEPFNVRYWGLGNEMYGRWQIGATEDPHDYVKRAREFAKVMRWIDPSIELVSCGQNGW
                     QDWNGVVIAGLAEYIRYHSIHIYTGNSEFAENVLEPHMAEYAIEACAAEIQRVRVQND
                     IKHEIRIAFDEWNVWYRAHSETMLEERYNLSDALAIATYLNGFVRNADTLAIANLAQM
                     VNVIAPIVTSPDGLFLQTIYHPLALAAASLQGMALDAWYDGPRIAPPESQTRPVTDRL
                     RKLGDFPLVDAAATLRDDSTVTLSLVNRSEGDDVELTVDLGVDSVSAILSIDEINGPD
                     VDAVNDFGRELVTTTSRGATGSGRTVTIALSPHSYTVVRLAVGG"
     gene            complement(2418..>2630)
                     /gene="rpmB"
                     /locus_tag="VFQ54_09845"
     CDS             complement(2418..>2630)
                     /gene="rpmB"
                     /locus_tag="VFQ54_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU0165334.1"
                     /translation="ECNRRETAVAGKCDVCGKAKTFGNNVSFSKRRTNRDFRPNVQKK
                     RFVVDGVGVRINICTRCLRTLGKTAA"
BASE COUNT          468 a          872 c          812 g          478 t
ORIGIN      
        1 cggaacgtcg cgctgaagat gcgcggcggc tcctcgaacg gggctacacc gcgatcaagc
       61 ttcgcctcca cgacgagaca atgcggcagg acatcgcgca ggtcgaggcc gtccgtgacg
      121 ccgtcggcga ccggatgacg attcttgtcg atgcgaacca ggcgcaggaa ccgggcacgc
      181 ctggtgccga acgcagcatt ttctggggat acaatcgagc cctcgcgaca gcccgggaac
      241 tccagcaact tggcgtcacc tggctggaag aaccactgcc ccgctacgac ttcgacaacc
      301 agcggcggct cgcggcggca accgatctcc cgatcgcggg cggcgagaac aacatcgggc
      361 tccatgagtt ccggacgctg atcgacacgg ggtgctacga cgtgctgcaa ccggatgcac
      421 tcgtctgcgg cggcatcagc atgctgcgga agattgccgc ctacgccgag atgcatggca
      481 aaccggttgc accgcatcac ggtggcaacg ggctcggggt cgccacgcac ctgcacctga
      541 gtgcatcgct accgaactcg ccgtgggtgg agctgctgca ggacccgccc gcgatggagg
      601 cttccgagtt ccagggaatc ctcgccacgc cactcgtgcc ggggaaggac ggcatcgtgc
      661 cgttgcccga ggcgcccggc cttggcgtcg agttggcaga acacgtcctc gccgcgctgc
      721 cagggtgatg ccttcaggac gcatcgagat cgtctgatca gccgccgacc gcgagccgga
      781 cgacggtgta ggagtggggc gacaacgcga tcgtcacggt ccggcccgaa ccggtcgcgc
      841 cccggcttgt cgtcgtcacg agttcgcggc caaagtcgtt gaccgcatcg acatccgggc
      901 cattgatctc gtcgatcgac aggatcgcgg acacggaatc cacaccgagg tcgaccgtca
      961 gctcgacgtc gtcgccctcc gatcggttca cgagggagag cgtgaccgtc gagtcgtcgc
     1021 ggagcgtcgc cgcggcatcg acgaggggaa agtcgcccag cttccggagg cgatcggtca
     1081 ccggtcgggt ctgcgattct ggcggagcga tccgcggacc gtcataccag gcatccagcg
     1141 ccattccttg cagggaggcc gccgccaacg cgagcgggtg atagatcgtc tgcaggaaga
     1201 gaccgtccgg cgaggtgacg atcggcgcga tcacgttcac catctgcgcg agattcgcga
     1261 tcgcgagcgt atccgcgtta cggacgaagc cgttgagata cgtcgcgatc gccagcgcgt
     1321 cagagaggtt gtaccgctcc tcgagcatcg tctccgaatg cgcccggtac cagacgttcc
     1381 actcgtcgaa cgcgatcctg atctcgtgct tgatgtcgtt ctggacgcgg acgcgctgaa
     1441 tctcggccgc acacgcctcg atcgcgtact ccgccatgtg cggttcgagc acgttctccg
     1501 cgaactcgga atttccggta tagatgtgga tgctgtgata gcggatgtat tcggcgagac
     1561 cggcgatcac gacgccgttc cagtcctgcc agccgttctg gccacaactg accagttcga
     1621 tcgacggatc gatccagcgc atgaccttgg cgaactcacg cgcccgcttc acgtagtcgt
     1681 gcgggtcctc ggtcgcgccg atctgccacc tgccgtacat ctcgttgcca agaccccagt
     1741 agcgaacgtt gaacggttcg tcgtgaccgt tcgcccggcg ctggttcgcc cagtatgtat
     1801 ttcccgtacc gttgcaatac tcgacccagt tctgggcctc ctcgaccgtt cccgtcccca
     1861 tgttgacgca gatatacggc tcggcgccga tcgcgcggca gtactcgatg aactcgtccg
     1921 tgccgaaccg gttcgattcc tcctgccgcc acgccagctc gatccgtcgc ggccgctcat
     1981 ccttcgggcc gatgccgtcc gtccagtggt agccactgac gaagttgccg ccgggccagc
     2041 gcaggatcgg catctgcagc cccgcgacct ctgccatcac gtccgtccgg aacccacgag
     2101 cgtcgctcaa cggcgagcct tcttcaaaga caccgccgta gatgcagcgc cccaggtgct
     2161 cgatgaatcc tccatagatt cgcggatcga ccggcgcgat gcggcgttcg aggtcgatct
     2221 tcaggcgtgc gctgagtgtc acggtctccg gccctccggg aagtggaatc ggtgaatgca
     2281 attgtcggaa ctacgacgat tgtagcaacc ggtcctcatc tcgcaaacgg ccgcggaaga
     2341 cacaatgagg cggatacctt gcggtacccg cctcatcgct tcatctcacc tggttttttc
     2401 ggaggatcgc cgaccgatca ggcagcggtc ttgccgaggg tgcgcaggca acgggtgcag
     2461 atgttgatcc gcacgccaac gccgtccacc acgaagcgct tcttctggac gttcgggcgg
     2521 aaatcccgat tcgtccggcg cttggagaag ctcacgttgt ttccaaatgt cttggccttg
     2581 ccacagacgt cgcacttacc ggccacggca gtctcccttc gattgcattc
//
LOCUS       DASTMD010000668         7402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1312161, whole genome shotgun sequence.
ACCESSION   DASTMD010000668 DASTMD010000000
VERSION     DASTMD010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312161"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VFQ54_09850"
     CDS             complement(<1..786)
                     /locus_tag="VFQ54_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0165335.1"
                     /translation="MVEREGESGVEVAVAVATIGERPPRSLRSRAAHAGEDGGRSSGP
                     HVEPIHQSAVYAFADAEAAGMAYAKGDPLYARDGLPNVRALERAVADLEGAEDAQAVS
                     SGMAAIALSLTSLLTAGDHVVAPACGYCDTSALLEELLVRFGIRVTFADYHDAANVEA
                     AIEARTRIVFAETISNPGMELADLPALAAIAHRNGVLLLVDNTFATPLLCRPLEHGAD
                     LVLHSAGKFLGGHSDVTAGVVAGREDLIATMRRHAYLTGPTLAP"
     gene            complement(858..2516)
                     /locus_tag="VFQ54_09855"
     CDS             complement(858..2516)
                     /locus_tag="VFQ54_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0165336.1"
                     /translation="MPWIRRITTIVAGLLAGIVAACFLALLMVAARTWLGISPPPESV
                     PDRIAPTLSINHFFSLFGKYGGYNGLKKYGVKTGIQALFGAGAVVGVLYAIVSETGFS
                     RRIGAWRFGVSRIGAIFLAVLTLIVWLGSVIFLWPVLQTNFRGLPPSQARYVTIAGLF
                     LEYLGFTVMLALTYWFVVRHARIAAERTDSGSDATAVTTGSPAVELGRPLARRSIVAA
                     GAGAVFAYPAYRLIKRLYDRAVFPYDGTVYSGPGVMPITPNDKFYTVTKNVVDPNVNK
                     AVWGLDIFGMVDRHHTYSYDDIVAMPQVKQETTLMCISNAIGAGLSSNAVWTGIPMAE
                     LLNKAGVQEGAVEVKLYGADGYTDTFAIEKALDPTTMVVYQMNGEPLAERHGYPVRLV
                     VPGLFGEKNVKWVTGIEVIDHDGKGFYEEQGWGPDFEVPTRSDIFSPKRTRHSGQDSF
                     DQTLPVNKPVTLKGRAFAGARGVKTVEVSLDNGETWQQAHFDYPGTKLTWTFWSYVWT
                     PEKTGDYVLICRATDDTGALQTTKVRGTAPDGATGLHKVKAKVI"
     gene            2769..3542
                     /locus_tag="VFQ54_09860"
     CDS             2769..3542
                     /locus_tag="VFQ54_09860"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEU0165337.1"
                     /translation="MEDWCGDDAHRQFAYRLRFDWGLRGAQAIAPDADIAIVVDVLSF
                     TTTLSVALDEGIVVLPYQWKDESAARYAREHKATLAVSRKTAARGTITLSPQSIRDAV
                     PVPRLVLPSPNGSAISFRLAEGRSTVLGASLRNADAIATWIAINYDAAATNISVIAAG
                     EQWADGSLRPAVEDLWGAGAVLSALVARGWTDLSPEAQVAQHAFLGVKESIERQLQRC
                     ASGRELIDRGFASDVEIAAEFNSSASVPLLRDRRFVPAP"
     gene            3576..3908
                     /locus_tag="VFQ54_09865"
     CDS             3576..3908
                     /locus_tag="VFQ54_09865"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU0165338.1"
                     /translation="MAIGRALPEVEESTSYGTPALKVKGKLLARLRSDGDGGLMLRCA
                     LEEKELLLQSGDPSFYTTPHYDGYAAILIDLERVDVTRLTEMIDEAWYLQAPVKVRKL
                     REAQQQDG"
     gene            3901..4854
                     /locus_tag="VFQ54_09870"
     CDS             3901..4854
                     /locus_tag="VFQ54_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU0165339.1"
                     /translation="MADTRLTLDAIANARDVIDPIFLNTPQYVDAALSAQLGCEVLIK
                     IETLNPIRCFKGRGASLYMQEAVAAGERVVGASAGNFGQAIAYAGRARGVPVTIFAAE
                     TANAAKIARIRDLGAEVVLTGRDFDAAKEAARAYAVSAPEVRFVEDAADIETVIGAGT
                     IGMELAAGSLDALLVPLGNGALINGIATWLKAHSPQTRIIGVVAANAPSMEISWRTGV
                     PTTTETASTIADGIGVRIPIASAVDLTTRLVDEIVLVEENTIREALRLIRDAAGLLLE
                     PSGAVGVAALLARPELRTGKVGTILTGSNYAANLLAELTGA"
     gene            4921..5595
                     /locus_tag="VFQ54_09875"
     CDS             4921..5595
                     /locus_tag="VFQ54_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEU0165340.1"
                     /translation="MTEATSPSTHYAQAKARIIDLVTAPDVNLDAPVPACPDWVVRDL
                     IAHMIGVPADFVNGRMENAPSPQWTAVQVAARRGRSLAELLAEWDEQTDAIAPVLESP
                     MGMNLICDAAVHEQDLRGALGEVGERENPIITTASGLLLGRLRAAIERGDAPALRVVI
                     DGEANILGDGEPAGELRASAFELLRSTSGRRTPDQVRALFTSGDPDLWIEHFFVFGPR
                     TTPLIE"
     gene            5766..6524
                     /locus_tag="VFQ54_09880"
     CDS             5766..6524
                     /locus_tag="VFQ54_09880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0165341.1"
                     /translation="MMAEPRVESVESLLGDHPLDNVIWSALTSRQRHLAEGNAEDGAL
                     RYQREFAPFAALHDTSPQSFAALDRMLASESPASLVPLFTTIPITPPESCTNLAVALR
                     SPIHQMVATTMPDVSLPTIGEIVTLGADDIPEMLALVDLTKPGPFAARTHEMGTYLGI
                     RVNGQLAAMTGERFHLDGFTEVSAVCTHPDHRGKGYGALLVATIANGILSRGEIPFLH
                     VFATNTPAIGLYEKMGFAIRRTLELTVLGGDHAS"
     gene            complement(6533..6964)
                     /locus_tag="VFQ54_09885"
     CDS             complement(6533..6964)
                     /locus_tag="VFQ54_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0165342.1"
                     /translation="METAAIVGTLDLCYCTTLRKAARRISQFYEEKLQPTGLRVTQFA
                     MLRVIGESREIAINELATRLELDRTTTGKNLRPLERAGLIAVKPSSTDGRSRTVALTP
                     EGDAILIVATPLWNEAQQEFERNLGGDAAALRDRLGALGIE"
     gene            complement(7104..>7402)
                     /locus_tag="VFQ54_09890"
     CDS             complement(7104..>7402)
                     /locus_tag="VFQ54_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165343.1"
                     /translation="IGRAYYAAYLEARSFCERHLSYVKSSTSREHQDVAMLMRRVDDT
                     IADDLRFLRGFRNAADYDIRLSPTTILLSSNDAVQLAIQIISSLDLHATRMAQQ"
BASE COUNT         1368 a         2457 c         2294 g         1283 t
ORIGIN      
        1 cggagcgagg gtcggtccgg tgagataggc gtgccgccgc atcgtcgcga tcagatcctc
       61 ccgaccggcg acgacgcctg ccgtgacatc gctgtggccg ccgaggaatt ttccggcact
      121 gtgcagcacc agatcggcac cgtgttcgag cgggcggcag agcagcggcg tcgcgaaggt
      181 gttatcgacg aggagcagca cgccgtttcg gtgggcgatt gccgcgagcg cggggagatc
      241 ggcaagttcc atcccgggat tcgagatcgt ctccgcgaag acgatccgcg ttcgcgcctc
      301 gatcgccgct tccacattcg cggcatcgtg atagtcggcg aacgtcacgc ggataccgaa
      361 ccgcaccagt agttcctcga ggagcgccga ggtgtcgcag tacccacagg caggcgcgac
      421 cacatggtcg ccggcggtca gcagcgaggt caggctcagt gcgatcgccg ccatcccgga
      481 cgacaccgcc tgcgcatcct ccgcgccctc gagatcggcg accgcgcgct cgagggcacg
      541 gacattcggg agtccatccc gcgcgtagag cggatcgccc ttcgcatagg ccatccccgc
      601 cgcctcggca tcggcgaatg catagaccgc gctctgatgg atcggctcga cgtgcggccc
      661 ggaagaacgc ccgccatcct caccggcatg ggcggcgcgg ctccgcagcg aacgcggtgg
      721 acgctcccct atggtcgcaa ccgcaactgc aacctcgact ccgctctcgc cttcccgctc
      781 gaccatctgc tcccgaccct tccgatgcga accagacgat accgatgcgg ccacggccag
      841 actccccaga ctcccgtcta gatgaccttc gctttcacct tgtggagacc ggttgcaccg
      901 tccggcgcgg ttccgcgaac cttggttgtc tggagcgcgc cggtatcatc cgtcgcccgg
      961 cagatcagca cgtagtcacc ggtcttctcc ggcgtccaga cgtaactcca gaaggtccac
     1021 gtcagcttcg tgcccgggta gtcgaagtgc gcctgctgcc acgtctcgcc gttgtcgagg
     1081 ctgacctcca ccgtcttcac gccgcgggcc ccggcgaagg cacgtccctt caaggtgacg
     1141 ggcttgttca ccggcagcgt ctgatcgaac gagtcctggc cgctatgacg cgttcgcttc
     1201 ggcgagaaga tgtcggagcg cgtcgggacc tcgaaatccg gaccccaccc ctgctcctcg
     1261 tagaagccct tgccatcgtg gtcgatgacc tcgatcccgg tcacccactt cacgttcttc
     1321 tcgccgaaga ggccgggcac gacgagccga accggatagc cgtgacgctc ggcgagcggc
     1381 tccccgttca tctggtaaac caccatcgtg gttggatcga gcgccttttc gatcgcgaag
     1441 gtgtcggtgt agccatcggc gccatagagc ttcacctcga cggcaccctc ctggactccc
     1501 gccttgttca gcaactccgc catcgggata ccagtccaca cggcattgct cgagagcccg
     1561 gcgccgatcg cgttgctgat gcacatcagc gtcgtttcct gcttcacctg cggcatcgcg
     1621 acgatgtcgt cgtagctgta ggtgtggtga cgatcgacca tcccgaagat gtcgagcccc
     1681 cagaccgcct tgttaacgtt cggatccacg acgttcttgg tgacggtata gaacttgtcg
     1741 ttcggggtga tcggcatcac gccgggaccg ctgtacaccg tcccgtcgta gggaaagacg
     1801 gcgcggtcgt agagacgctt gatcaggcgg taggcgggat aggcgaagac cgcgccggcg
     1861 ccggccgcga cgatcgagcg acgcgccagc ggtcgtccca gttcgaccgc cggcgaaccg
     1921 gtggtgacag ccgtcgcatc cgaaccggag tccgttcgtt ccgccgcgat acgtgcatgt
     1981 cgaacgacga accagtacgt gagcgccagc atcaccgtga aaccgaggta ttcgaggaag
     2041 agcccggcga tcgtcacgta tcttgcctgg ctcggcggga gcccgcggaa gttcgtctgc
     2101 aacacgggcc agaggaagat cacgctgccg agccagacga tcagcgtgag gaccgcgagg
     2161 aagatcgccc cgatccggct gaccccgaat cgccaggcgc cgatccgccg cgaaaacccg
     2221 gtctcgctca cgatcgcgta gagcacccca acgaccgcac cggcgccgaa gagtgcctgg
     2281 atacccgtct tcaccccgta tttcttgaga ccgttatagc caccgtactt gccgaagaga
     2341 ctgaagaagt gattgatcga gagcgtcggc gcgatccggt ccggcaccga ttccggcggc
     2401 ggcgagatac cgagccacgt tctcgccgcg accatcagca gcgccagaaa gcatgccgcg
     2461 acgatcccgg cgagcaagcc cgcgacgatc gtggtgattc tgcggatcca cggcataggt
     2521 ctgctccctg acgacgtccg gagacgtgac tcgatgcgca ccgtcgcgcg tcgcctgaat
     2581 actacagaac ggtctgtagt cttttcaacg tgatatcgac atcgctgaat ctatcctgac
     2641 ccacgaacgc aaacgaccgc ggatatgaaa caatcagtcg caaggggcag atctcgcacc
     2701 aggcttcagg ccgtcatgag gcaatacgtt cagggtgggt aacctggatc acccggagga
     2761 ccgtcagtat ggaagattgg tgtggcgacg atgcacaccg ccagtttgcc tatcgcttgc
     2821 gttttgactg ggggctgcgt ggcgcccagg caatcgctcc cgatgccgac atcgcgatcg
     2881 tcgtcgatgt cctctccttc accacgacgc tgagcgtggc gctcgatgag gggattgtcg
     2941 tcctgcccta tcaatggaaa gacgaatcag cggcacgcta cgcgcgggag cacaaggcaa
     3001 cgctggcggt cagtcgcaaa accgcggcac gaggcacgat caccctctcg ccacaatcga
     3061 tccgcgatgc ggtgccggta cctcgcctgg tgttgccctc gccgaacggt tcagccatct
     3121 cctttcgcct cgccgagggt agatcgacgg tcctcggagc aagcctgcgc aacgccgatg
     3181 ccatcgcgac ctggatcgcg atcaattacg acgcggcagc gacgaacatc agcgtgatcg
     3241 cggcgggaga acagtgggcg gatggatcgc tccgtccagc cgtcgaagac ctctggggtg
     3301 ccggcgccgt gctttcggcg ctcgttgccc ggggctggac cgatctttcc ccggaggcgc
     3361 aggtcgccca gcacgcattt ctcggcgtca aggagagcat cgagcgtcag ctccagcgct
     3421 gcgcgagcgg gcgcgagctg atcgatcgcg gcttcgcgtc cgacgtcgag atcgccgccg
     3481 agttcaactc cagcgcatcg gtgccgcttc tgcgcgaccg ccgctttgtg cccgccccct
     3541 gatcggagga acgaatggtg acatggagcg aggtcgtggc aatcggacgg gcgcttcccg
     3601 aagtcgaaga atcgacctcc tacggaacgc cggcgctgaa ggtgaaaggc aagctcctgg
     3661 cgcgcctgcg ctctgacggc gatggcggcc tcatgctcag atgcgcgctg gaggaaaagg
     3721 aactcctgct ccagtcaggc gacccgagct tctacacgac accacattac gacggctacg
     3781 cggcgatcct catcgatctc gaacgggtcg acgtcacccg cctcaccgag atgatcgacg
     3841 aagcctggta tctgcaggca cccgtcaagg ttcgcaagct ccgggaggcg caacaacagg
     3901 atggctgaca cccgactcac gctcgatgcg atcgccaacg cacgcgacgt catcgatccg
     3961 atcttcctga acacgccgca atacgtggat gccgcactct cggcgcagct cggttgcgag
     4021 gtcctgatca agatcgagac cctgaaccct atccgctgct tcaaggggcg cggcgcctcc
     4081 ctctatatgc aggaggcggt cgcggcgggt gagcgcgtcg ttggcgcctc cgccggtaat
     4141 ttcgggcagg cgatcgccta cgccggacgc gcgcggggcg tcccggtcac gatcttcgcc
     4201 gcggaaaccg cgaacgccgc caagatcgcg cggatccgcg acctcggtgc ggaagtcgtg
     4261 ctcacgggtc gtgacttcga cgccgcgaag gaagccgcgc gcgcctatgc cgtctcggct
     4321 ccggaagtcc gcttcgtcga ggatgcggcc gacatcgaga ccgtgatcgg cgcgggcacg
     4381 atcgggatgg aactcgcggc cgggtcactc gatgcactgc tcgtcccgct cgggaacggc
     4441 gccctgatca acggcatcgc gacgtggctg aaggcgcatt cgccgcagac gcgaatcatc
     4501 ggcgttgtcg cggcgaatgc gccgtcgatg gagatcagct ggcgaaccgg agtgccgacg
     4561 acgacggaga ccgcgagcac gatcgccgac ggcatcggtg tccgcattcc catcgcgagc
     4621 gcggtcgacc tgacgacacg cctcgtcgac gagatcgtgc tggtcgagga gaacacgatc
     4681 cgggaggcgc tccgcctgat ccgggatgcc gcgggcttgc tcctcgagcc atccggcgcg
     4741 gtcggtgtcg ccgcacttct ggcgcgaccc gaactccgaa ccggcaaggt cgggacgata
     4801 ctgaccggtt ccaactacgc ggccaacctc cttgccgaat tgaccggtgc ctgacagaat
     4861 cgagacggtc tccccgcgac tcgtacccgc gtccgattag cgcgatggag aaacagcccg
     4921 gtgaccgaag ccacttcgcc gtcaactcat tacgcccagg caaaagctcg catcatcgac
     4981 ctcgtcaccg cgccagacgt gaatctggat gcgccggttc cggcctgtcc ggattgggtc
     5041 gttcgtgatc tgatcgccca tatgatcggc gttcccgcgg atttcgtgaa tggtcggatg
     5101 gaaaatgcac catcgccgca atggaccgcc gtccaggtcg cggcgcggcg cggcaggtcg
     5161 ctcgccgaac tcctcgcgga atgggacgag cagaccgacg cgatcgcacc tgttctcgaa
     5221 tcgccaatgg ggatgaacct catctgcgat gccgcggtcc acgagcagga tcttcgaggc
     5281 gcgctcggtg aggtcgggga gcgagagaac ccgatcatca cgaccgcaag cgggttgttg
     5341 ctcgggcgtc tcagggccgc aatcgaacgt ggagacgcac ccgcgctgcg cgtcgtgatc
     5401 gacggtgaag cgaacatcct cggcgatggc gagcctgcgg gtgagctgcg ggcatcagca
     5461 ttcgaactgt tgcgatccac gtctggccgc cggaccccag accaggtccg cgcgcttttc
     5521 acgagcggtg atcccgacct ctggatcgag cacttcttcg tcttcgggcc gcgcacgact
     5581 cctctcatcg aatagcaccc gccgactcca gcaactgaca ggtcggccga tggctcgaag
     5641 atgggctcga gaagctcaag gtcgttgctc gcgtaatata ggggcatata ctactatctc
     5701 gaggatcgac atgacgcggc acggttgacc ggaacgccgg aaccacgaaa gatgatgagg
     5761 caccaatgat ggcagaaccc cgggtggaat cagtggaatc gcttctgggg gatcatcccc
     5821 tcgacaatgt gatctggagc gcgctcacct cgcggcagcg tcatctcgcc gaaggcaatg
     5881 cggaagacgg cgcgctccgg taccagcggg agttcgcccc gttcgccgcg ctccatgaca
     5941 catcgccgca gagcttcgcg gcgctggacc gaatgctcgc aagcgagtcc cctgccagcc
     6001 tcgtgccgct cttcacgacc atcccgatca cgccaccgga gagttgcacg aacctggcgg
     6061 tcgcactccg ctcacccatc catcagatgg tggcaacgac gatgcccgac gtctcgctcc
     6121 ccaccatcgg cgagatcgtg accctcggcg ccgacgatat ccccgagatg ctcgcgcttg
     6181 tcgacctcac gaaaccgggg cccttcgccg cgcgaaccca tgagatgggc acctacctcg
     6241 ggattcgggt caacggccaa ctcgcggcga tgaccggcga acgcttccat ctcgatggct
     6301 tcaccgaagt cagtgctgtc tgcacgcatc ccgatcaccg cggcaaggga tacggggcgt
     6361 tgcttgtcgc gaccatcgcg aacgggattc tatcccgcgg cgagatcccg ttccttcacg
     6421 tcttcgccac caacaccccg gcgatcgggc tgtacgagaa gatgggtttc gcgatccgcc
     6481 ggacgctgga actcacggta cttggcgggg accacgcgtc ctgacgccgg gatcactcga
     6541 tgccgagcgc gcccagccgg tcgcgcagcg ccgccgcatc gccaccaagg ttccgttcga
     6601 actcctgctg cgcctcattc cagagcgggg tcgcgacaat caggatcgcg tcgccctccg
     6661 gcgtcagcgc cacggtccgg ctgcgaccat ccgtcgacga tggcttcacg gcgatcagac
     6721 ccgcccgttc gaggggacgg agattcttgc cggtcgtcgt ccgatcgagt tcgaggcgag
     6781 ttgccagttc gttgatcgcg atctcccgcg attcgccgat cacgcggagc atcgcgaact
     6841 gggtgacgcg cagacccgtg ggctgcagct tctcctcata gaactgcgag atccggcgcg
     6901 ctgccttgcg gagcgtggtg cagtaacaga gatcgagggt accgactatt gctgcagttt
     6961 ccatctacct gacgcccatt cgtggaactc gctgaccgac cattcgttcg ccaagcatag
     7021 gtgactccgc tgttacgtgc atatactcgc atgcatgagt tacctgcagc ggtgatctcg
     7081 gcccggtcat caagcaaggc cgattactgc tgcgccatcc gtgtggcgtg cagatcgagc
     7141 gacgagatga tctggatcgc gagctgaacc gcatcatttg aactcaacaa gattgtcgtc
     7201 ggggagagac ggatatcgta gtcagcggca tttcggaagc cacgcaggaa tctcaagtca
     7261 tctgcgatcg tgtcgtccac tcgccgcatc aacatcgcta cgtcctgatg ctcgcgggag
     7321 gtcgagcttt tcacatagga caagtggcgc tcacagaacg acctcgcttc gagataggcg
     7381 gcatagtagg ctctgccaat cc
//
LOCUS       DASTMD010000669         1963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1758130, whole genome shotgun sequence.
ACCESSION   DASTMD010000669 DASTMD010000000
VERSION     DASTMD010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758130"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1249
                     /locus_tag="VFQ54_09895"
     CDS             245..1249
                     /locus_tag="VFQ54_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0165344.1"
                     /translation="MTQVGIRDVAERAGVSVGTVSNVLNGNRAVASSTVGRVRAAIDE
                     LGYVRNDVARQLRAGRSTTVGFVALDARNPFFADIARGAEDEASKRDLVVVVGNSDQR
                     LDQEARYLDAFAEQRVRGILISPLGDVSSRIEQLHVRGIPAVLVDRFSPDHRVSSVSV
                     DDVAGGRMAVAHLLESGRRRIAFVGGPEEIRQVADRLKGARSVVNAAGADLEVIPAEG
                     LTVLHGRAAGEELAGRRPSDRPDGIFAANDLVALGLLQSLVMRGGIRVPEDLSLIGFD
                     DIDFAASAVVPLTSIRQPSLLMGRTALQILEAEASPGVASPQQLVFDPELVIRRSTNR
                     "
     gene            complement(1366..1854)
                     /locus_tag="VFQ54_09900"
     CDS             complement(1366..1854)
                     /locus_tag="VFQ54_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165345.1"
                     /translation="MTESWSTLEEVHAARARFEEAILGWRRPEAFGVCIVTQTASSGI
                     DVNLPVVTVRTHYLAAAILAGVVGHVSGDEALPLSVEQLSQAIELLAPASAYPKSDHP
                     NIAAWRRLHREASASTTLLAVFLDELRPEIDPKIALLRVAVNRWPAPIRRLTSESPAR
                     FR"
BASE COUNT          354 a          622 c          640 g          347 t
ORIGIN      
        1 tccgacacct tctcgaaggg atcgcggggc acccctggct gggcgaacac cttgaagcgg
       61 gttccggagt tgccgaatgc ccaggagggc agctcgattg cctgctcggc taggcgaggg
      121 gcgatggatg tgaagtcggt catgatgaaa cccttcgccg atattgaatc gtttcaattc
      181 tggactgtac agagagccac gcttctcgtc aaactgttcg ggtggaaccc gagggaagac
      241 gcgcatgacc caggtcggga ttcgcgacgt tgcggagcgc gcgggcgtct cggtcggtac
      301 ggtctccaat gtcctcaacg gcaaccgagc cgtggcgtca tcgaccgtcg gacgcgtgcg
      361 cgccgcgatc gatgagctcg ggtacgtccg caacgacgtg gcacgtcagc tacgcgcggg
      421 acgcagcacc actgtcggct ttgtcgcgtt agacgcccgc aacccgtttt tcgccgatat
      481 cgcccgggga gccgaggacg aagcatctaa gcgagacctc gtcgtagtcg tcggcaattc
      541 tgaccagcgg ttggatcagg aggctagata cctcgatgcg ttcgctgaac aacgcgtccg
      601 cggcatcctg atctcgcccc tcggcgatgt ctcgagccga atcgagcaac tccacgttcg
      661 cggtattccc gccgttctcg tcgaccggtt cagcccggac catcgcgtca gctccgtctc
      721 cgtcgacgac gtcgcgggag gtcgaatggc ggtcgctcat ctgctggaga gcggacgtcg
      781 ccgaatcgcg ttcgtcggcg gcccggagga aatccgccag gtggcggatc gactcaaagg
      841 ggccagaagc gttgtcaacg ccgccggtgc cgacttggaa gtgatccccg cggaggggct
      901 gaccgtactt cacggtcgcg ccgcgggtga agaactggcg ggacgccgac cttccgatcg
      961 cccggacggc attttcgcag ccaacgatct cgttgcgctc ggcctccttc agtcgctcgt
     1021 catgcggggc gggatacggg ttccggaaga tctgagcctg atcggattcg acgacatcga
     1081 cttcgcggcg tccgcagtcg ttcctctcac ctcgatccga cagccgagcc tattgatggg
     1141 cagaacagca ctgcagatac tggaggccga agcatccccg ggcgtggcgt ctccgcagca
     1201 gctggtgttc gacccggagc tggtaatccg ccgttcaacc aaccggtgac caacaaatgg
     1261 tcaggccacc ttcggtacat cgactaccgc aacgggttcg tgtcagttgc ggattcccgg
     1321 ggcagctagc gatcgcggcc gcgtagggca ttaccgtggt cgcgatcacc ggaaccgggc
     1381 cggagactcc gaagtcaaac gacggatagg cgccggccat cgattgacgg cgacccggag
     1441 tagggcgatt tttgggtcaa tctccggtcg caattcatcg agaaacacag cgagcaaggt
     1501 cgttgaggca ctcgcctctc ggtgaaggcg tcgccaggcg gcgatgttcg ggtggtccga
     1561 cttggggtag gcgctcgccg gggcgagcaa ctcgatcgcc tgactcagct gctcgactga
     1621 cagagggagt gcctcgtcac cggaaacatg cccgacgact cccgcgagga tcgcagcggc
     1681 caggtagtgc gtccgcacgg tgacaacggg caagttcacg tcgattccac tcgacgcggt
     1741 ctgcgtcacg atgcagaccc cgaatgcctc tgggcggcgc cagccgagta tcgcctcctc
     1801 gaagcgggcg cgggcagcgt ggacctcctc gagggtggac caggattcgg tcatccgtcc
     1861 cgacccgatc gtgcttggac ccggtcggct cgaggcgcga accgaccgta gcctgcaccc
     1921 attgggaact ctccgcggtc gaacacccag cgcccgcgca cca
//
LOCUS       DASTMD010000670         1841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_240233, whole genome shotgun sequence.
ACCESSION   DASTMD010000670 DASTMD010000000
VERSION     DASTMD010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240233"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VFQ54_09905"
     CDS             complement(<1..826)
                     /locus_tag="VFQ54_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165346.1"
                     /translation="MNSDNSNDSIKSQSILRRKYSRRTVMRAGVVTTAAGLLLPRGAF
                     AQGATPAATPDANGVLVSAVEGVPNAYTKTPTPFQSVASIPGGGDKVRMLTLSYSPPP
                     TEKGSNSYWQELEKRLGVTWDANLVPVASYGEKTSAIIASGDIPELFYLLVTSSAPVI
                     AQSISQGAWKDLTPYLTGDAIKDYPNLALYPSYIWDAVKTDGKIYGVPKPVLRSNDTS
                     FYRTDWLNKLGLKAPETTDDVHDMLVAFSKGDPDGNGNADTFGVTAYDASWSLFNWN"
     gene            complement(900..1637)
                     /locus_tag="VFQ54_09910"
     CDS             complement(900..1637)
                     /locus_tag="VFQ54_09910"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0165347.1"
                     /translation="MTRLDREGSVPLYLQVKAWLEEQIATGKLKPHRRVPSERDLSER
                     FEISRMTVRQALTEMVHDGRLYSRVGMGTFVADPLIRQPLRSLTGFTEDMRTRGSVPS
                     SRILRCELVTMPAHLRSVFPVESSRTVVALERLRLADDRPLMVETTHLSFPHVERVVG
                     ADLSGSLYAHLKDTFGIYPAAADQEVIARRARPREIALLDMDEETAVLEFTRVTSDRA
                     GKVFEHVVSVYRGDRYRFVIRLEAEGG"
BASE COUNT          346 a          569 c          596 g          330 t
ORIGIN      
        1 ggttccagtt gaagaggctc cacgacgcat cgtaggccgt gacgccgaag gtatcggcgt
       61 tgccattgcc atcgggatcg cccttcgaga aggcgacgag catgtcgtgc acatcgtcgg
      121 tcgtctccgg cgccttcaga ccgagcttgt tcagccagtc ggtgcgatag aacgacgtat
      181 cgttgctgcg caggaccggc ttcggcacgc cgtagatctt gccgtcggtc ttcaccgcat
      241 cccagatgta ggacgggtag agcgcgagat tcggatagtc cttgatcgcg tcacccgtga
      301 gatacggcgt caggtccttc caggcaccct gcgagatcga ctgtgcgatg accggggcac
      361 tgctcgtcac gagcaggtag aagagctccg ggatgtcgcc gctcgcgatg atcgccgagg
      421 tcttctcgcc gtagctggcg accggcacga gattggcgtc ccaggtcacg ccaagccgct
      481 tctccagctc ctgccagtag gagttgctgc ccttttccgt cggtggcggc gaataggaaa
      541 gcgtcaacat ccggaccttg tctccgccgc ccgggatcga tgcgacggac tggaatggtg
      601 tcggggtctt ggtgtaggcg ttcggcacgc cctcgaccgc agaaacgaga acgccgttcg
      661 catccggtgt ggcggccgga gtcgcgccct gggcgaatgc gccgcgtggg aggagcagcc
      721 ccgcggccgt cgtcacaacg cccgctcgca tcacggtgcg gcggctgtac ttgcggcgaa
      781 ggatcgactg ggacttgatc gagtcgttgg agttgtccga gttcatggca aaacctcatg
      841 cggtgcgatg tgagtcgtca gggtcccttc gcgccggtcg cgcgcattct cagagcgatc
      901 taccctcctt cagcctccag acggatcacg aaacggtagc ggtcgccgcg atagacagaa
      961 acgacgtgtt cgaagacctt gccggcacga tcgctggtca cacgggtgaa ctcgagaacg
     1021 gcggtttcct cgtccatatc gagcagagcg atctcccgcg gtcgggcacg acgggcgatc
     1081 acttcctgat cggccgccgc cggatagatg ccgaacgtgt ccttcaggtg ggcatagagc
     1141 gaaccagaga gatccgcacc gacgacacgc tcgacatgcg gaaacgagag atgtgtcgtc
     1201 tcgaccatca gcggacgatc gtcggccagc cgcagtcgct ccagcgcgac gacggtgcgt
     1261 gacgactcga cgggaaagac ggaccgaaga tgggccggca tcgtgacgag ttcgcaacgc
     1321 aggatccggc tcgatggaac cgatccccgg gtccgcatgt cctcggtaaa ccccgtcagg
     1381 gaccgcaacg gctgccggat caggggatcg gcgacgaagg ttcccatccc gacgcgggaa
     1441 tagagacgcc cgtcgtgaac catttccgtc agcgcctggc gaaccgtcat ccggctgatc
     1501 tcgaaccgct cgctgagatc gcgttcggaa ggcacgcgcc ggtgtggctt gagcttgccc
     1561 gtcgcgatct gctcctcgag ccacgccttg acctggagat agagggggac agatccctca
     1621 cgatcgagtc gcgtcatcgg cgtacgagtt cccctcgcga aagctatcca ccccagtccg
     1681 gtctagacca atcctagccg gatcattgag gaaccgtcaa cggggtgccg ggaaccgaag
     1741 aggagtgtca accggtgtct tggtactgat cgcacgaagt cagaggctca gaacttggtg
     1801 ggagggaccg cttggtcacc gcgtgctcac cgcgcaatcc c
//
LOCUS       DASTMD010000671         1878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_418483, whole genome shotgun sequence.
ACCESSION   DASTMD010000671 DASTMD010000000
VERSION     DASTMD010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418483"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFQ54_09915"
     CDS             <1..692
                     /locus_tag="VFQ54_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU0165348.1"
                     /translation="MEIAREPGLRSKVAVAARQEKVDPVGSCVGVRGVRIQNIVNELY
                     GEKIDVIEWSSDMSTFIANALSPAKPTNVSLNEDGDQRVATVIVPNDQMSLAIGKEGQ
                     NARLAYKLTGWRIDIKGPESLLQSGTDFFRNAREAANDMMPDFSWQGRQPRAVQADGM
                     VALSGRSYGPLSEDLIRRQVDVDIVGGIVEVYYDRELRARFDQVTGDRLPLDEETDEF
                     GYSSAGEHARS"
     gene            711..1082
                     /locus_tag="VFQ54_09920"
     CDS             711..1082
                     /locus_tag="VFQ54_09920"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU0165349.1"
                     /translation="MPQHAARPQPSKNKSRQVPRPKHVPRRTCVACRESDDKRSLTRI
                     VRTPEGTVEIDPTGKKNGRGAYLCDRRSCWERAVSTPVLARALNVEQLPEETLIALRQ
                     FAAGLSETPADAPEISKEPTI"
     gene            1079..>1878
                     /locus_tag="VFQ54_09925"
     CDS             1079..>1878
                     /locus_tag="VFQ54_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEU0165350.1"
                     /translation="MTDTQSRSGGRSGGGNRGGNARRNGGRRTGGARTVAVKRLTPVV
                     REPVTLPPVLSVAELAEKIETSGIEVIRELMKIGIMASLNQQIDYSTAARVAEALGWE
                     TNEQETEAKSTVADFASRRLEGENDPDAVARPPVITIMGHVDHGKTKLLDTIRSANVA
                     EGEAGGITQHIGAYQVDAQGRKLTFLDTPGHEAFTAMRARGAQSTDIAVLVVAADDGV
                     MPTTREAVAHIKSANVPMVVAINKIDLPAANPDRVKQQLSEMEVMPEE"
BASE COUNT          377 a          564 c          637 g          300 t
ORIGIN      
        1 tcatggagat cgcccgtgag ccgggcctgc gctccaaggt cgccgtggcc gcacgccagg
       61 agaaggtcga tccggtcggt tcctgcgtcg gggttcgcgg tgtgcgcatc cagaacatcg
      121 tcaacgagct ctacggcgag aagatcgatg tgatcgagtg gtcgtcggac atgagcacgt
      181 tcatcgcgaa cgcgttgagc ccggcgaagc cgacgaacgt ctctcttaat gaggatggcg
      241 accagcgggt ggcgaccgtg atcgtgccga acgaccagat gtcgcttgcg atcggcaagg
      301 aagggcagaa cgcccgcctc gcctacaagc tgacaggttg gcggatcgac atcaagggcc
      361 cggagtcgct gctccagtcc ggaaccgact tcttccgcaa cgctcgcgaa gccgcgaacg
      421 acatgatgcc cgacttcagc tggcagggac gacagccccg cgccgtgcag gcggacggca
      481 tggtggcgct cagcggccgg tcttacgggc cgctgtccga ggatctcatt cgccgccagg
      541 tggatgtcga tatcgtcggc ggcattgtcg aggtgtacta cgatcgcgaa ctccgtgccc
      601 ggttcgacca ggtgacagga gatcggttgc cgctcgacga ggagacggac gagttcggct
      661 attcctcggc aggcgagcac gcgcgatcct gataagggag gtgtgcgagt atgcctcagc
      721 atgcagcccg gccccagccg agcaagaaca aatcgcgaca ggtgccgcgg ccgaaacacg
      781 tgccgcggcg aacctgcgtc gcatgccggg agtcggatga caagcgttca ttgacgcgaa
      841 tcgtccggac ccctgaagga acggttgaga tcgatccaac cggtaagaag aatggacggg
      901 gggcatatct ttgtgatcgt cggagttgct gggaacgggc ggtgtcgacg ccggtactgg
      961 cgcgggcact caacgtcgag caactccccg aagaaaccct gatcgcccta agacaatttg
     1021 ctgctggctt gtccgagacc cccgcggacg cgccagagat ttcaaaggag ccaacaatat
     1081 gacagacacc cagagccgca gtggcggacg aagtggtggt ggcaaccgcg gtggcaacgc
     1141 ccgccgtaat ggtggccgtc gtaccggtgg cgcacgcacc gttgccgtca agcgcctgac
     1201 tccggtcgtg cgcgagccgg tcacgctccc tccggtcctt tccgttgcgg agctcgcgga
     1261 gaagatcgag acatccggga tcgaagtgat ccgggagctg atgaagatcg gcatcatggc
     1321 cagtctgaat cagcagatcg attactccac ggccgcccgc gtcgccgagg cgctcggatg
     1381 ggagaccaac gagcaggaga ccgaggcgaa gtccacggtc gccgacttcg cgagccggcg
     1441 actcgaaggc gagaacgatc cggatgcggt cgcgcgtccg ccggtgatca cgatcatggg
     1501 acacgtcgac cacggcaaga ccaagcttct tgacacgatt cgctcggcga atgtggcgga
     1561 gggcgaggcc ggcggtatca cccagcacat cggtgcgtat caggtcgatg cccagggtcg
     1621 caagctgacc ttcctcgaca cgccgggtca cgaggcgttc accgccatgc gtgcccgtgg
     1681 cgcccagtcg acggatatcg cggtcctcgt ggtcgcggcg gacgacggcg tgatgccgac
     1741 gacgcgtgaa gcggtcgccc acatcaagtc ggcgaacgtg ccgatggtcg tcgcgataaa
     1801 caagatcgac ctgccggcgg cgaatccgga tcgtgtgaag cagcagcttt ccgagatgga
     1861 agtgatgccg gaagaatg
//
LOCUS       DASTMD010000672         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1492280, whole genome shotgun sequence.
ACCESSION   DASTMD010000672 DASTMD010000000
VERSION     DASTMD010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492280"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /gene="fabF"
                     /locus_tag="VFQ54_09930"
     CDS             <1..1187
                     /gene="fabF"
                     /locus_tag="VFQ54_09930"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU0165351.1"
                     /translation="VASTFAAVRDGGSGLGPITRFDLDGFETTFAAEVKGFDPTVVGR
                     KEHRRMDRFTQFAIVASREAVAQSGLDIRADPTRVGVLLGTGLAGAETFESTVEILLT
                     KGPRRISPFAVPMAIPNMAPGMVAIDIGAKGPNFAYSSAFAGSANAIGEAARMILDDV
                     CDAAIAGGAEAPVSRMGVAGFSAMGALSTRNDDPIHAIRPFDLTRDGCVLGEAGAVLL
                     LEDRDHALARGATILAELVGYGASADAFHLVALPPDGEGLVSAMRLAIEDAGIAPDQI
                     GYLNAHGTATEMNDRVETVAIKRVFGDHAAKLPISSTKAATGHTLGAAGSLESIIAIE
                     AMRCGMLPPTLNQIERDPICDLYYIPNVARSASVDYVMSNSMGFGGHNAVLIYANVDR
                     AV"
     gene            complement(1285..>2689)
                     /locus_tag="VFQ54_09935"
     CDS             complement(1285..>2689)
                     /locus_tag="VFQ54_09935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165352.1"
                     /translation="LLAWQLMVVLDGTIVNIALTHIRGSLGFSETGLSWVVNAYALAF
                     GGLLLLGGRAGDILGRRRVLVIGVLIFTFASFLGGIAPNAELLLSARVLQGIGAAIAA
                     PSTLSLIIVNFHEPADRNRALSLFSSISGVGASIGLIMGGVLTSWLSWHWVFFVNIPI
                     GLAVAFLAPRTVTESPRVPGKFDLVGALTSTVGVTSLVYGFIRASEEGWTDLLTLGAF
                     ALAVVLLVGFVLIEARAAQPIMPLRLLANRNRLFGYMNMLLVPSAMFGLFFFLTQFLQ
                     NVLGFSPLRTGVAFLPLSASIITISRFAPRLVARFSARNVMSAGATLLVIALLWLSRV
                     NTGSGYFQDIVAPVILLGLGAGLSFTPLTMTILSNLDPRDSGSASGLLQTMQQIGGAV
                     GLAVLVTVFGTASRNATGDGKSPLEAMTHGTGQGFLTAAGLAVIALLIAIFGISRIKK
                     EQPEATTAPSAEPVSLH"
BASE COUNT          526 a          870 c          908 g          385 t
ORIGIN      
        1 acgtcgcctc cacattcgcc gccgtccggg acggcggctc cgggctcgga ccgatcacgc
       61 gcttcgatct cgacggcttc gagaccacct tcgccgccga ggtcaagggt ttcgacccca
      121 ccgtcgtggg acgcaaggag caccggcgga tggatcgctt cacgcagttc gcgatcgtcg
      181 cgtcgcggga agcagtggcc cagtccggac tcgacatccg cgcggatccg acacgcgtcg
      241 gcgtcctcct tgggaccggg ctcgccggcg cggagacgtt cgaatcgacg gtggagatcc
      301 tgctgacgaa ggggccgcgc cgcatctcgc cgttcgccgt gccgatggcg atcccgaaca
      361 tggcgccggg gatggtcgcg atcgacatcg gggcgaaggg gccgaacttc gcctactctt
      421 ccgccttcgc tggatccgcc aatgccatcg gcgaggcggc gcggatgatt ctcgacgacg
      481 tctgcgacgc cgcgattgcc ggtggcgcgg aagctcccgt ctcccggatg ggcgtcgccg
      541 gcttcagtgc gatgggagcg ctctcgacac gaaacgacga ccccatccac gcgatccgtc
      601 ccttcgatct cacgcgcgac ggatgcgtcc tcggggaggc gggagcggtc ctgctgctcg
      661 aggaccggga tcacgcgctc gcccggggcg ccacgatcct cgccgagctc gtgggctacg
      721 gcgcatccgc cgatgccttc catctcgtgg cgcttccgcc agacggggag gggctcgtct
      781 ccgcgatgcg actggcaatc gaggatgcgg ggatcgcccc tgaccagatc ggctacctga
      841 atgcccatgg caccgcgacc gagatgaacg atcgcgtgga gaccgtcgcg atcaagcgtg
      901 ttttcgggga ccacgccgcg aagctgccca tcagctcgac gaaggcggcg accggccaca
      961 ccctcggggc ggcaggatcg ctcgaatcga tcatcgcgat cgaggcgatg cgctgcggca
     1021 tgctgccgcc caccctcaac cagatcgagc gcgatccgat ctgcgatctc tactacatcc
     1081 cgaacgtggc tcgttccgca tccgtggact atgtgatgtc caactcgatg ggattcggcg
     1141 gacacaacgc cgtgctgatc tacgcgaatg tcgaccgggc tgtctgacat cccgtcccga
     1201 cgcgaccggc atgtctcgca cagacaaagg aagggcgctc ccgatcggga gcgcccttcc
     1261 ttcatatctg ggtcgagtaa gcgatcagtg cagcgagaca ggctcggcgc tcggggcggt
     1321 cgtcgcctct ggctgctcct tcttgatccg gctgatgcca aagatcgcga tcaggagggc
     1381 gattacggcg agaccggcgg ccgtcaggaa gccctgaccc gtgccgtgag tcatcgcctc
     1441 gagcggcgac ttgccatcac cagtcgcatt gcggctggcc gtgccgaaga ccgtcaccag
     1501 caccgcgaga ccgacggcgc caccaatctg ctgcatcgtc tgcaggagac cgctggccga
     1561 accggagtcc ctcggatcga ggttggacag gatcgtcatc gtcagcggcg tgaacgagag
     1621 gccggcgccg aggccaagca gaatcaccgg cgccacgatg tcctggaagt agccactgcc
     1681 cgtattgacg cgggagagcc acaggagtgc gatcacgagg agcgtcgccc ccgcgctcat
     1741 cacgttgcga gccgagaagc gggcgaccag gcgcggggcg aaccgggaga tcgtgatgat
     1801 cgacgcggaa agcgggagga acgccacacc ggtgcgcagg gggctgaagc cgaggacgtt
     1861 ctgcaggaac tgcgtcagga agaagaagag accgaacatc gccgatggca ccagcagcat
     1921 gttcatgtag ccgaagaggc gattgcgatt cgcaagcagc cgcagcggca tgatcggctg
     1981 cgccgcacgc gcctcgatca ggacgaagcc gaccagcagg acgacggcca acgcgaaggc
     2041 accgagggtc agaagatcgg tccatccctc ttccgaagca cgaatgaagc cgtagacgag
     2101 tgaggtgaca ccgaccgtcg aggtgagcgc gccaaccagg tcgaacttgc ccgggactcg
     2161 cggcgattcc gtcacggtgc gcggcgcgag gaacgcaaca gcgagaccga tcgggatgtt
     2221 gacgaagaag acccagtgcc aggagagcca tgaggtcagg acgccgccca tgatcaggcc
     2281 gatcgaggcg ccgacaccgg agatgctcga gaagaggctc aacgcacgat tgcggtcggc
     2341 cggctcatgg aaattgacga tgatcagcga gagcgtgctt ggggcagcaa tcgcggcgcc
     2401 aataccctgg aggacgcggg ccgagagcag caactcggca ttcggcgcga tcccgccgag
     2461 gaaggacgcg aacgtaaaga tgaggacgcc gatcaccagc acgcggcggc gaccgaggat
     2521 gtcaccggca cgaccgccga gaagcagcaa tccaccgaag gcgagcgcgt aggcgttgac
     2581 gacccacgac agcccggtct ccgagaagcc gaggcttccg cggatgtgcg tgagagcgat
     2641 gttcacgatc gtgccatcga gcacgaccat cagctgccaa gcgagaagg
//
LOCUS       DASTMD010000673         1646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3188585, whole genome shotgun sequence.
ACCESSION   DASTMD010000673 DASTMD010000000
VERSION     DASTMD010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3188585"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFQ54_09940"
     CDS             <1..186
                     /locus_tag="VFQ54_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165353.1"
                     /translation="KLSLSRQVRRCEAEESLHEIEVHSRGSRRGRWERGSTARANWSL
                     DCGPATRCARDPGLRSG"
     gene            complement(431..856)
                     /locus_tag="VFQ54_09945"
     CDS             complement(431..856)
                     /locus_tag="VFQ54_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165354.1"
                     /translation="MLSRRTVVRDDVATARFHGTPDLDPTRRRGVMNVVKALVNQRSL
                     FRENGKISLMGDRLLIETWQGEVLDLRRSDIAEISNEFSKEYGDYIGGAVKAWGAPVH
                     VALNDGRSLYLLINQRWFSERTENREWTRRLDAWWSRTR"
     gene            complement(875..1429)
                     /locus_tag="VFQ54_09950"
     CDS             complement(875..1429)
                     /locus_tag="VFQ54_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEU0165355.1"
                     /translation="MEYGRTTTSNLKQVFFWMHCALFTGINSVLFVINMLVDRSNPWF
                     LYPLWGWGMVLAFQAGFTYPWRGWFGAHLAATVVLLAGLTGINIVFGDGPWVIWVLLA
                     LGIPLLAHALYVFRRATLFQSQAIASVLWFAMIVYVQFHYDGVWWSSLLSILTSGSVF
                     MGLLMLHRTFRHYLPERYDDPLTR"
BASE COUNT          346 a          538 c          489 g          273 t
ORIGIN      
        1 aagctgagcc tgtcccggca ggtgcgccgc tgcgaagccg aagaatcgct ccacgaaatc
       61 gaagtccact cccgtggcag ccggcgcgga cgatgggagc ggggaagtac cgctcgtgcc
      121 aactggtccc tcgactgcgg cccggccact cgctgcgctc gtgatccggg actccgctcg
      181 ggatgacgga aaaggatacg gatgtggtca cgtcgcgagg cgggacacca aggtcccgcg
      241 ccctcggctg ggcccggggc aagctcccga acgaccgtcg tatcgacgat cgtgtatggg
      301 gttccccaag ctcggaacgt ggttggaacc gccggggttt gtagggcggg agtttatctc
      361 tcgccgcgag cgcggcatcc ccactcatcg tgccttgcgg acgaggtcgt ccccgcgttg
      421 tccctccccg tcagcgggtg cgactccacc aggcatccag ccgcctggtc cattcccgat
      481 tctcggtgcg ctcgctgaac cagcgttgat tgatcagcag gtaaagcgag cgcccgtcgt
      541 tgagggcgac gtgaaccggc gctccccacg ctttgaccgc accaccgatg tagtcgccat
      601 attccttcga gaactcgttc gagatttccg cgatgtcgga acgacggaga tcgaggacct
      661 caccctgcca ggtctcgatc aggagccgat cgcccatcag cgagatcttg ccgttctcgc
      721 ggaagagcga gcgctggttg acgagcgctt tcacgacatt catcaccccg cgtcgccgcg
      781 tcggatcgag atcgggtgtc ccatgaaaac gggcagtggc gacatcatcc cgaaccaccg
      841 tgcgtcgtga aagcatcgtc cctccatttc acccctatcg cgtcaatgga tcatcatacc
      901 gttccggaag atagtggcga aaggtacggt gcagcatcag cagccccatg aacaccgacc
      961 cgctcgtaag gatcgacagg agcgacgacc accacacccc gtcgtagtga aactggacgt
     1021 agacgatcat cgcgaaccag agcacgctgg caatcgcctg cgattggaag agtgtcgccc
     1081 tgcggaacac gtagagcgca tgcgcgagga gcgggattcc aagggccagc aggacccaga
     1141 tcacccacgg accgtctcca aagacgatgt tgatcccagt caatcccgca agcagcacga
     1201 cggtcgccgc gagatgggca ccgaaccagc cacgccaggg ataggtaaag ccagcctgga
     1261 atgccagaac catgccccaa ccccagagcg gatagaggaa ccacgggttc gagcggtcga
     1321 caagcatatt gatgacgaag agaacgctgt tgatccccgt gaacagcgcg cagtgcatcc
     1381 agaagaacac ctgcttcagg ttcgatgtcg tggtacggcc atactccatt cccgatgcct
     1441 ccaacatagc gcccgcacga ccagctccat tcgttggctc cgtcgagagt gccatcgctc
     1501 tgctccttcc cgcgttcacg gcgacgccat cagattggcg ccatcccata cgtcgagtct
     1561 agagacgagc aatcatgccg aacatgcagg ggaccgccga tcgcggggac ggttatcccc
     1621 cacggtcgac ggccccgggc aacgaa
//
LOCUS       DASTMD010000674         4196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_240508, whole genome shotgun sequence.
ACCESSION   DASTMD010000674 DASTMD010000000
VERSION     DASTMD010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240508"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(995..2320)
                     /locus_tag="VFQ54_09955"
     CDS             complement(995..2320)
                     /locus_tag="VFQ54_09955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0165356.1"
                     /translation="MSISEYTGPSVFGYETDQPVCRAAIICLHTSPIERIGQRDAGGM
                     NVYVKNLAIQLARQGIQVDIFTRRTDPLLPEVVEVTPGVNVIHITAGPPEPRPKNELF
                     VYLPEFANEAALYSLRAQVRYDVIHAHYWLSGWAAHLLQRYWHVPHLLMFHTTAHMKN
                     AVAATADRETPLRMEIERKLLDLADGIVAANPDERTDLISRMHTPIDKVCTVPPGVDL
                     RIFQPGSRADARRQLGIGLDEKIVVSVGRVDPIKGFDTLLHAMQQLKAHRQPGTLILA
                     GGDLDEAGNPAHTLAGIAAQAEMLGVRDRVRFVGSQSHEQLATFYRAADVVAVPSRYE
                     SFGLVAIEAMASGTPVVASNVGGLRFTVENDRSGYLVPHSDPTALADAMTKILTDDAL
                     RDRLGEGAVVQANRFAWESVGEQIERMYRLLISGYQDELCGAGAVTLFA"
     gene            complement(2422..2745)
                     /locus_tag="VFQ54_09960"
     CDS             complement(2422..2745)
                     /locus_tag="VFQ54_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165357.1"
                     /translation="MVSESTLAIPATRIDVSDLIAFHPSIRVMVGKVHASVDDTSFAS
                     TEPAVWLWSTDGRIRPTFVQEGTLEQSLQSAHLQLRKFFCGASRSQREADEAEGGIII
                     PDYMY"
     gene            complement(2920..3486)
                     /locus_tag="VFQ54_09965"
     CDS             complement(2920..3486)
                     /locus_tag="VFQ54_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165358.1"
                     /translation="MNDERDSRKGAAAPVQTFLLPENHPFPNNADLVAIYYPHGVVLD
                     REDPAGSMEAHFAANSWGYSWRNGIFPYHHYHSTAHEVLGIAAGTARVRLGGDGEIGV
                     EIEMSAGDTLVIPAGVALKNLGSSQDLVAIGAYPPGQLWDVNTGEDDERPRTDEKIAH
                     LPLPTTDPVHGESGPLIDFWLAHHARSL"
BASE COUNT          723 a         1274 c         1446 g          753 t
ORIGIN      
        1 aggggggcga gaagtgcgtc ctgcgcagtc atcgtcacga tgacgcgggg acgggagccg
       61 ttcagggtcg ggtgtggact gtcgatcgcc attgataacg tccttttcgt tcggttggtg
      121 gctgcggatg tcgtgcgcgg caatcatccg tgatggtggc gcgggacgcc agaccccgga
      181 cagtacctgc ccggtctctg gcgtcggccg aggtcgcttc gcgatgagca agcccacctc
      241 cgaactcgcc gaggaatcgg ctcgcctcga cgtgcttccc gtagccagcc tcacacacca
      301 cgtcctcccg agctcgtcga gggatcagcc agcggctacg tgcttccggc acggctgggt
      361 tgtcagacgt ggggtgagca ggcgaggcat cgttgacggg atgatcaggg cgaccacgtg
      421 ggtcgccgga tgggtgaggc gggttgccgg acgaggcggt cgatggcgcc gcctcgggtc
      481 aatgcggcgg cgtcgatccg tctcccgggg acgccgcgcg cgacgtcccg accgccagca
      541 tcaggcggga cattcctccg cccactgacc ggaccttccg tcccaaagac gcgccgatcg
      601 ccggtcgctg ggctccgtag tcccgcccca cgcacgaccg agcacatccg cgggctcttc
      661 catacgcgac gcgctcccgg tcgcggctcg gcgatgccgc gttgagccgg aacggcgaca
      721 cgcgccgtgg ggacgaaaat gagacgggcg tggcgtcccg atcagccggg atctgcgggg
      781 cgaggattta tctctcgccg cgaggtcgga ggtggggcaa cattgcttcc gctccacgat
      841 cgcccgcgaa gatcggggtt ggtagggcgg ggctttatgc cccgccgcag acgcgaaagt
      901 gttgatggga tgaccagggc gaccacgtgg gtcgccggga cgggtgcagc aagggcgccg
      961 gggcgacgcg agattcgccc cggcgagacc ttggctaggc gaagagggtg acggcgccgg
     1021 cgccgcagag ttcgtcctga tagccggaga tgagcaggcg gtacatccgc tcgatctgct
     1081 cgccgacgct ctcccaggcg aagcggttcg cctggacgac ggcgccctcg ccgaggcggt
     1141 cgcggagcgc gtcatcggtg aggatcttcg tcatcgcgtc ggccagcgcg gtcggatcgg
     1201 aatgggggac gaggtagccg ctgcggtcgt tctcgaccgt aaagcggagg ccgcccacgt
     1261 tcgacgcgac gaccggcgtg ccgctcgcca tcgcctcgat cgcgacgaga ccgaacgact
     1321 cgtagcgact cggcaccgcg accacgtcgg ccgcgcgata gaaggtcgcg agctgctcgt
     1381 gcgactgcga gccgacgaaa cggacgcgat cgcgcacgcc gagcatctcg gcctgggccg
     1441 cgatcccggc gagcgtgtgc gccggattgc ccgcctcgtc gagatcgcca ccggccagga
     1501 tcagcgtccc cggctgacgg tgcgccttga gctgctgcat cgcatggagg agcgtgtcga
     1561 agcccttgat cggatcgacg cgaccgacac tgacgacgat cttctcgtcg agcccgattc
     1621 cgagctgccg tcgcgcatcg gcgcgcgatc ccggctggaa gatccgcaga tcgacgcctg
     1681 ggggaaccgt gcacaccttg tcgatcggag tgtgcatccg ggagatgagg tcggtgcgct
     1741 cgtccgggtt cgcggcgacg atgccgtcgg cgaggtcgag gagcttgcgc tcgatctcca
     1801 tccggagcgg tgtctcgcgg tccgccgtgg cggcgaccgc attcttcatg tgagctgtcg
     1861 tgtgaaacat caacagatgg gggacatgcc agtagcgttg aagcagatgg gccgcccaac
     1921 cggagagcca gtagtgagcg tggatgacat cgtaacggac ctgcgcacgc aatgaataga
     1981 gcgcggcctc gttggcgaat tccgggagat agacgaagag ttcattcttg ggccggggtt
     2041 cgggtggccc tgcggtgata tggatgacgt tgacgccggg ggtgacttca acgacttcgg
     2101 ggaggagggg atcagtacga cgggtgaaga tatcgacctg gatgccctgg cgggcgagtt
     2161 ggatggcgag gttcttgacg tacacgttca taccaccggc atcgcgttga ccgatgcgct
     2221 cgatgggcga ggtgtgcagg cagataatgg cggcgcggca aacgggctga tcggtttcgt
     2281 atccgaagac cgacggaccc gtgtactccg agatggacac ggtgattcgt cctttcaaac
     2341 tgttcgaatg tgcggtgccg ggtaccgcaa cttcccgggt gaagtatcgc acccatggct
     2401 cgatcttggc ggtctcgcgg cctagtacat gtagtccggg ataatgatcc caccctcggc
     2461 ttcatccgct tctcgctggc ttcgcgaggc gccgcagaag aatttccgaa gctggagatg
     2521 cgccgattgc agactctgtt cgagcgtccc ctcctgaacg aaggtcgggc ggattcgacc
     2581 gtccgtcgac cagagccaga ccgccggctc cgtcgatgcg aacgatgtgt catcgaccga
     2641 cgcatggacc ttgccgacca tgacgcgaat gcttgggtgg aaggcgatca gatcgctgac
     2701 gtcgatcctg gtcgccggga tcgcgagcgt ggattcggac accatatgtg tacgacggct
     2761 ccttcatcat cagatgcgtt ggggcgagcg atgccccgtg cttcagcggg ctgcgacaac
     2821 ggagccctcg ctctattctt gccgatcgga agccacttgg caaaatgaag cgccctgcgc
     2881 gacaggattc gtcgtgaaga atcgccccgg ggacggaatc tacaggcttc tggcgtgatg
     2941 cgcgagccag aaatcgatga ggggaccgct ctcgccgtgg acgggatcgg ttgtcggcag
     3001 gggaaggtgg gcgatcttct catcggtccg gggacgctcg tcgtcctcgc cggtgttcac
     3061 atcccagagc tgaccgggag gataggcgcc gatcgcgacg agatcctgtg atgaaccgag
     3121 gttcttcagg gcgacaccag ccgggatcac gagcgtatcc ccggcggaca tctcgatttc
     3181 gacaccgatc tcgccgtcgc cgccgagcct gacgcgcgcg gttcccgccg cgatgccgag
     3241 gacttcgtgt gcggtgctgt ggtaatggtg gtaggggaag atcccgtttc gccaggaata
     3301 gccccagctg ttcgcggcga agtgcgcttc catcgaaccg gctggatctt cgcgatcgag
     3361 aacgacgccg tgggggtagt agatcgcgac gaggtcggca ttgttgggga atggatgatt
     3421 ctcgggaagc agaaaggtct ggacaggcgc cgctgcgcct ttccgggaat cccgttcgtc
     3481 attcatgatg ggcgcacgat ccttcgattg cgtggtgctg gacacagacg ttctgtaccc
     3541 ggaatggaat tgtacgttca gtcgccgact tcggccgcac atctcacacc atcgcacgaa
     3601 ttcccatgcc gtcaagacga cggcgtctga gccgcctgcg ccgcgctccc gggcgtgcgc
     3661 tcccggtctg gacggagtaa cgtcctgcct cctcacggga cggtcatatc cggctgcgcc
     3721 ggatgggtca cacggggtga cccgctacgg gaatgtgaca gcgcgacgcg cgtcggaacc
     3781 atgctggctt cggccaagac cgcgcgcatc ttgcctgaca caccgcttgt tgctactatc
     3841 catggaagtc gcaacacata tagggctcat ttgtccgcat ctacgcaagg cagcgatggt
     3901 atttcatcga attgcgccgg cgggacggtc gttttcgatc gatcctccgg ttcggtgtcg
     3961 ttgacgtgac cgttgccgaa agctgcgggg aacgtggagc cgtgcgcttg cgatcagtcg
     4021 tgattgtaga aggagcaagc gaaatgccca gatccgagca ccaatcgacc gacgatcgta
     4081 tcgaacaatc cgcatcgaga cacggcggat tgagccgccg cgggttgctt cagaccggcg
     4141 ccgttgccgc ggcagcctcc accctcgcga tcggacccgt gcggcgggcc gtcgcc
//
LOCUS       DASTMD010000675         1988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2118234, whole genome shotgun sequence.
ACCESSION   DASTMD010000675 DASTMD010000000
VERSION     DASTMD010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2118234"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VFQ54_09970"
     CDS             complement(<1..701)
                     /locus_tag="VFQ54_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0165359.1"
                     /translation="MTEQQQPRVGIVGLGGIGRTHIAAWIANGITPVAFADTVPAALE
                     QATTTYGGTAYADAKDLIASGTVDIVSICTPPAFHAEIAIAAVEAGVATICEKPLAAN
                     LADAKRVADAVARTGTLFTVGFCHRFQPEVEKLKAMIEGGELGQIRSFRNRFAAHLKN
                     AEQTWFANPAISGGGALIDTSVHSIDLFRYLIGDPVQVQALMSTTANDLGPALPVEDT
                     AILSLATADGTLGVI"
     gene            complement(708..1580)
                     /locus_tag="VFQ54_09975"
     CDS             complement(708..1580)
                     /locus_tag="VFQ54_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0165360.1"
                     /translation="MPRFGLLGVNTSHADAFSRIFNGTADDAALIDGSEITTIWGNDL
                     ARIDELATQHHIANRVAHPNDMLGQVDGVLVVDDTNGGAIHADLARPFLEAGMPVFVD
                     KPMTTEYADAVALFEIAGRTGAPLLSCSSLRFAVELEAAREELAKIGKLSSVVSVGPG
                     DWFYYGVHAVELLGAVAGTGATTVHRHVFDQRDVVVVGYENGPTAVVETLRDSSYVFE
                     LTAYGEKGHLSFAVEDAIGFYSNTMREVVKMVETGISPLTPEQTLEVLAILHAGTRSA
                     ETGATVSLTDVHGA"
BASE COUNT          338 a          598 c          682 g          370 t
ORIGIN      
        1 tcgatcacac cgagcgtgcc gtccgccgtc gccagcgaga ggatcgcggt gtcctcgacc
       61 gggagcgccg ggccgagatc gttcgcggtc gtgctcatca atgcctgcac ctgcaccgga
      121 tcgccgatca gatagcggaa gagatcgatc gagtgaacgc tcgtgtcgat cagcgcgccg
      181 ccgccgctga tcgcgggatt cgcgaaccag gtctgctcgg cattcttcag gtgcgcggcg
      241 aagcgattgc ggaacgaccg gatctggccg agttcgccgc cttcgatcat tgccttcagt
      301 ttctcgacct ccggctggaa gcggtggcag aagccgaccg tgaagagggt gccggttcgc
      361 gcgacggcat cggcgacgcg tttggcatcg gccagattcg cggcgagcgg tttctcgcag
      421 atcgtcgcga ctcccgcctc gacggcggcg atcgcgattt cggcgtggaa tgcgggtggc
      481 gtacagatgc tgacgatatc gacggtgccg gaggcgatca gatccttcgc gtcggcgtat
      541 gcggtgccgc cgtatgtcgt ggttgcctgc tcgagtgcgg ccgggacggt gtccgcgaag
      601 gcgacgggcg tgatgccgtt ggcgatccag gcggcgatat gggtgcgtcc gatgccgccg
      661 agtccgacga tgccgacgcg tggttgctgc tgttcagtca tgatcgatca ggctccatgc
      721 acgtcggtga gagaaacggt cgcgccggtc tcggcggaac gcgtgccggc gtggaggatc
      781 gcgaggacct ccagcgtctg ctccggggtc agtggcgaga tgcctgtctc gaccatcttc
      841 acgacctcac gcatcgtgtt gctgtagaag ccgatcgcgt cctcgaccgc gaagctgaga
      901 tgtcccttct cgccatatgc ggtcagctcg aagacatagg acgaatcccg caacgtctcg
      961 acgacggcag tcggaccatt ctcgtagccg accacgacga catcgcgttg gtcgaagacg
     1021 tggcgatgga cggtcgtggc accggttcct gccacggcgc cgagcagctc gaccgcgtgg
     1081 acgccgtagt agaaccagtc gccggggccg acactgacaa ctgacgagag cttgccgatc
     1141 ttcgccagtt cctcacgcgc cgcctcgagt tcgaccgcga agcgaaggga cgagcagctc
     1201 agaagcggcg ctccggttcg accggcgatc tcgaagagtg cgacggcatc cgcgtattcc
     1261 gtcgtcatcg gcttgtcgac gaagacgggc attcccgcct cgaggaaggg gcgggcgaga
     1321 tcagcgtgaa tcgcgccgcc attggtgtca tcgacgacga ggacgccatc gacttgtccc
     1381 agcatgtcgt tggggtgggc gacccggttg gcgatatggt gttgcgtcgc gagttcatcg
     1441 atccgggcga ggtcgtttcc ccagatcgtc gtgatctcgc tgccgtcgat cagagcggcg
     1501 tcatcggcgg ttccgttgaa gatccgggaa aaggcatcgg cgtgcgatgt attgacgccg
     1561 agaagtccaa accgtggcaa ttgatactcc tgttcgttct tgtcatttcg actgaagcgc
     1621 agcggaatgg agaaatcccc gcgcgaagcg cagtcggcca acggccgaca tgaagctggc
     1681 atctgcgatg tctctccagc cgcaacacct ggaacaccgt aggtgcgacg cgtgtcgtcg
     1741 ccacgggtcg ccgaaggcga tccgtctcgc atgggaacac cgcgaatggc tggttcccga
     1801 tggggccggc tgcgccggtg cgcttcgcgc ggggatttct cggcaagctc gaaatgacag
     1861 ttgcatggga tgtccgggac cggctctgcc ggtcccggcg acgacaagcg tcgcgtctac
     1921 ggtttcgggt gcggatctgc aagcgcggcg tgggcaatgc gggcatcata tcgacctgcg
     1981 gtcgatgg
//
LOCUS       DASTMD010000676         3657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2383611, whole genome shotgun sequence.
ACCESSION   DASTMD010000676 DASTMD010000000
VERSION     DASTMD010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2383611"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFQ54_09980"
     CDS             <1..289
                     /locus_tag="VFQ54_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165361.1"
                     /translation="IALVLLVATLALGLSDSDRHLRLTVEDQASGAPVPGAMISVGDV
                     SYQTDTEGEIRIDAPKSGTVIVVTAEGYESMTGNFMSSSRLTQNVTLRARK"
     gene            353..1276
                     /locus_tag="VFQ54_09985"
     CDS             353..1276
                     /locus_tag="VFQ54_09985"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEU0165362.1"
                     /translation="MGDSRVGTAARVNSLVRASLLRPWIRLIRPVQWSKNAIVFAGVV
                     FARMATDPGELAKAILAFIMFCAISSAIYIFNDWQDIERDRHHPTKRARPLASGAIQP
                     RPALTVSILLAILGIAISVTVSWWLLLVCLGYLVMQVAYTLELKNVVILDVFIIAAGF
                     ILRAVAGAVAVDVPVSSWLLLCTFFLSLFLGFCKRRNEMHMLDDSAHAHRPSLKGYSI
                     PVLDQFIGLSAAVTLLSYSMYTFVSTYVPSNDAMMLTIPFVAFAISRYLYLVYGRNLG
                     GSPEVQLFRDLPLFFSIVAWGICVLGVLAFQ"
     gene            1418..1891
                     /locus_tag="VFQ54_09990"
     CDS             1418..1891
                     /locus_tag="VFQ54_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU0165363.1"
                     /translation="MIVVRRGRLGGDVASPSELEEFFRPVMSTRVPVRSANRGVWRPA
                     LEVFETTDALEVVAELAGMRADEIDIVIEGDVLTIRGNRQDPITCERRSYHEARIPYG
                     AFVAEVVVPFSVDADATEASYENGFLNVRLPRTRGRTIIATRSGSDGEKQNEAIQ"
     gene            1888..>3657
                     /locus_tag="VFQ54_09995"
     CDS             1888..>3657
                     /locus_tag="VFQ54_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU0165364.1"
                     /translation="MSNDETRDRAQGEPVAVDDEVVETPAAEPEESTVNAAAEDEAPT
                     AEERAERDVLADAENAPVIDGPAELPEEEILPLLPLRGTVVLPLTLVPLAAAQARSLR
                     LIDDVMAGERTVAMVMQKDADLAEGAGPDDILEIGTIATIHQMMRVPDGTVRLAVQGV
                     ERVKIVEVVQEEPYLKARVRRIPETVESTVEVQALMRNTLDLFQRLVSLVPHLPDELV
                     TAALNVDDDARHLVYLVASNLRMEPHERQELLEIDSVKEKLQTLNAFLTKELDVLELG
                     RKIQTDVQEELSKSQREYFLREQMKAIQKELGEGSEAEAEVAELREKIDASGMPEEAA
                     KEANRELDRLSKLPTAAAEYGVIKTYLDWLTSLPWNKSTESEINIPHARQVLDEDHYA
                     LDKIKDRILEYLAVRKLKQQNAEEGAEQLNREPILLLVGPPGVGKTSLAQSIARAMGR
                     EMTRMSLGGVRDESEIRGHRRTYVGAMPGRIIQAIRRAGSNDPVFVLDEVDKIGNDWR
                     GDPSSALLEVLDPEQNNSFRDHYLDVSFDLSKVMFIATANLLDTIPAPLRDRMEILNL
                     SGYTDEDKLNIAKRYLVPKQMKAN"
BASE COUNT          699 a         1076 c         1190 g          692 t
ORIGIN      
        1 cattgctctg gtgctgctcg tggccacgtt ggccctcggg ctctccgact ccgatcgcca
       61 tctgcgcctg accgtggagg atcaggcgtc cggagcgccg gttccgggtg cgatgatctc
      121 ggtgggcgac gtttcctatc aaaccgatac cgagggagaa atcaggatcg acgccccgaa
      181 gagcgggacg gtgatcgtgg taactgcgga ggggtatgaa tcgatgaccg gcaacttcat
      241 gtcgtccagc cggctcaccc agaacgtgac actgagggcg cgaaaatgag attgacctcc
      301 cctggcgacc gcctatcggc aatggcgcca ggagtattcc cgggcttgcg acatgggtga
      361 ttcgagagtg ggaaccgccg cgcgtgtgaa tagcctcgtc cgggcgagcc tgctcagacc
      421 gtggatccgg ctgatccggc ccgttcaatg gtcgaagaat gccatcgtgt tcgccggcgt
      481 cgtcttcgcc cggatggcga cggatccggg tgaactcgcg aaggcgatcc tggcgttcat
      541 catgttctgc gcgatcagca gcgcgatcta catcttcaac gactggcagg atatcgagcg
      601 cgaccgccac catccgacga agcgggcacg accgttggcc tcgggcgcga tccagccccg
      661 gcctgcgctg accgtgtcga ttctcctcgc catccttggt atcgcgatct ccgtcaccgt
      721 ttcctggtgg ctgctgctgg tctgccttgg ctatctcgtg atgcaggtcg cctacacgct
      781 cgagctgaag aacgtggtga tcctcgacgt gttcatcatc gcagcgggat tcatcctgcg
      841 cgccgttgcc ggcgcggtgg cggtggacgt tcccgtctcc tcgtggttgc tcctgtgcac
      901 cttcttcctc tcgctcttcc tcggattctg caagcggcgc aacgagatgc atatgctgga
      961 cgacagcgcc cacgcccacc ggccatcgct caaggggtat tcgattccgg tcctcgacca
     1021 gttcatcggg ctgtccgcgg cggtcacatt gctctcgtat tcgatgtaca cgttcgtctc
     1081 gacgtatgtt cccagcaacg acgcgatgat gctgacgatc cccttcgtcg ccttcgcgat
     1141 ctcccgttat ctctacctcg tgtatggacg aaacctcggc ggatcgcccg aggtccagct
     1201 cttccgggac ttgccattgt tcttctcgat cgtggcctgg gggatctgcg tgctcggtgt
     1261 tcttgcattc cagtagattc gtaccgcgaa acaggattga tacggaccgt agcaagttgt
     1321 tgacaaaaga ggtataatcc ttgcatgtcg agcgatgacg tctggccgta cccggcaggc
     1381 gtctcaagaa acgatccaga tatgcaaagg agtcccgatg atagtcgttc gacgcggtcg
     1441 cctgggaggg gatgttgcta gcccgtccga actcgaggag ttctttcgac cagtaatgtc
     1501 gacccgggtg ccggtgcgca gcgcaaatcg tggcgtgtgg cgtccggccc ttgaagtatt
     1561 cgagacgacc gacgctttgg aagtggtggc ggaacttgcg gggatgcgtg ccgacgagat
     1621 cgatatcgtg atcgaggggg acgtgctgac aatccgcggt aatcgccagg atccgatcac
     1681 atgtgagcgg cgttcgtatc acgaggcgcg aattccctat ggggcattcg tcgcggaagt
     1741 ggtcgttccg ttctcggtcg atgcggatgc gaccgaagcg tcgtatgaaa atggctttct
     1801 caacgtgcga ctgccgcgaa cgcgaggtcg gaccatcatc gcgacccgga gcgggagcga
     1861 cggtgagaag cagaatgagg caattcaatg agtaatgacg aaacccgaga tcgagcgcaa
     1921 ggcgagccag ttgcggttga cgatgaagtc gtcgagacgc cggcggccga gcccgaagag
     1981 tcgaccgtaa acgcggcggc tgaagacgag gcacctactg ccgaagagcg cgcggagcgc
     2041 gacgtccttg cggacgccga gaatgctccg gtgatcgatg gcccggccga actgccggaa
     2101 gaggagattc tcccgctgct gccgttgcgc ggcacggtgg tgcttccgct gaccctggtg
     2161 ccgctcgcgg ccgcccaggc gcgttcgctc cgtctgatcg acgatgtgat ggccggcgag
     2221 cgcactgtgg cgatggtgat gcagaaggat gccgacctcg cggagggcgc cggtcctgat
     2281 gacatcctcg agatcgggac gatcgcgacg attcatcaga tgatgcgggt gccggacggg
     2341 actgtccgtc tcgcggtcca gggtgtcgag cgcgtcaaga tcgtggaagt cgtccaggag
     2401 gagccctacc tcaaggctcg cgtccgccgg attccggaga cggtcgagtc gacggtcgag
     2461 gttcaggccc tgatgcggaa cacgctggat ctgttccagc gactggtttc gctggtgccg
     2521 catcttcccg atgaactggt gacagccgcg ctgaatgtcg acgatgatgc ccgccatctc
     2581 gtctacctcg tcgcatcgaa cctccggatg gagccgcacg agcggcagga gctgctcgag
     2641 atcgactccg tgaaggagaa gctgcagacg ctgaacgcct tcctcacgaa ggaactcgat
     2701 gttctggagc ttggtcggaa gatccagacc gatgtccagg aggaactgag caagtcccag
     2761 cgcgagtact tcctgcgcga gcagatgaag gcgatccaga aggaactcgg cgagggcagc
     2821 gaggccgagg ccgaagttgc ggaactccgc gagaagatcg acgcgtccgg gatgcccgaa
     2881 gaggccgcca aggaagcgaa tcgcgaactc gatcgcctga gcaagctgcc gaccgcagcg
     2941 gccgagtacg gggtcatcaa gacctatctc gactggctga cgagtctgcc gtggaacaag
     3001 agcacggaga gcgagatcaa catcccgcac gcccggcaag tgctcgacga ggatcactac
     3061 gccctcgaca agatcaagga ccggattctc gaatacctgg cggtccgcaa gctgaagcag
     3121 cagaacgccg aggaaggcgc ggagcagctc aatcgcgagc cgatccttct tctggtggga
     3181 cctccgggcg ttggcaagac ctcgctcgcc cagtccatcg cgagggcaat gggtcgcgag
     3241 atgacgagga tgtcgcttgg cggcgtccgt gacgagtcgg agatccgggg ccatcgccgc
     3301 acctatgtcg gtgcgatgcc gggccggatc atccaggcga tccggcgggc aggttcgaac
     3361 gatccagtct tcgtgctgga tgaggtcgac aagatcggca acgactggcg cggtgatccg
     3421 tcgtcggcgc tgctggaggt gctggatccg gagcagaaca acagcttccg cgatcactac
     3481 ctcgacgttt cgttcgacct ctcgaaggtg atgttcatcg cgacggcaaa cctgctcgat
     3541 acgattccgg ccccgctgcg ggaccggatg gagatcctca acctgagcgg ctacacggat
     3601 gaggacaagc tgaacatcgc gaagcgctac ctggtgccga agcagatgaa ggcgaac
//
LOCUS       DASTMD010000677         3332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3007484, whole genome shotgun sequence.
ACCESSION   DASTMD010000677 DASTMD010000000
VERSION     DASTMD010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3007484"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFQ54_10000"
     CDS             <1..414
                     /locus_tag="VFQ54_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165365.1"
                     /translation="STATILHQGEDGYGALMLLRLPAEAPTPVHNHNSWGVACVVEGT
                     NRYWRWERLDDLADPDRAEIRLVETFEHGPGEYAMWGDPPQDLHAQQGVGEAAYEFVF
                     FGRNPNLQPRAYFDPGTGTVTYSAATDAMAAPSSY"
     gene            511..1065
                     /locus_tag="VFQ54_10005"
     CDS             511..1065
                     /locus_tag="VFQ54_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU0165366.1"
                     /translation="MPDQDARVEEVRELRKVRQNREYTPELVSDEDLNELLEVARWTG
                     SSQNTQPWHFIVVRDKASLKAIGDCRPNIAWVADSPLAIALVLNGTNPPSEYYDEGRI
                     TERLLIAARYLGLGAGTAWLGEEPQQTTIKQVLGIPQEHILRSVVVIGHPQPGATHKL
                     GRTTSGRKPLDEVVSFETFDEHGN"
     gene            1109..1654
                     /locus_tag="VFQ54_10010"
     CDS             1109..1654
                     /locus_tag="VFQ54_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU0165367.1"
                     /translation="MPEYDSREISSFLRGLRQRRRFTDEIIPYEVIDDILEVARWTGS
                     AKNNQPWDLVVVRDRDTLAQLAESGTFAGFLRGVDVAIVIALNAGGVGQLYDEGRLSE
                     RIMLIAEAYGLGSGTGWFSEEPGQTRVKELLGIPQDRFVHSAVGLGYIDTSVDDRPNV
                     TLGRRPLSDVVSYGRFGQHEA"
     gene            1921..2661
                     /locus_tag="VFQ54_10015"
     CDS             1921..2661
                     /locus_tag="VFQ54_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU0165368.1"
                     /translation="MDFDVLQKLFSIVIVNLVLSGDNAVVIAMAARRLPEENRRKAIL
                     WGGAGAIVLRIILTAIAALLLDIPLLQAIGGILLVYISIRLITPEPEHHDAEVKQPGS
                     IGQAVQTILVADLVMSLDNIIAVGGTAEGELGLLLIGLVLSIALILVGSSLVAKLLEQ
                     YPWLLILGVLVLLHAAVKMFLHDHFVEKLFGYHHAPEWVSLAIAPVLTVIVLVFVRAW
                     RGGIAGFTDRDRAAVTAARANDPSTTRA"
BASE COUNT          569 a         1087 c         1066 g          610 t
ORIGIN      
        1 tcgaccgcga cgatcctcca tcagggcgag gatggctacg gcgcgctgat gcttctccgg
       61 cttccggcgg aggcgccgac gcccgtgcac aatcacaact cgtggggcgt cgcctgtgtc
      121 gtcgagggca cgaatcgcta ctggcgatgg gaacggctcg acgacctggc agaccccgat
      181 cgcgccgaga tccggctcgt cgagaccttc gagcatggac ccggtgagta cgcgatgtgg
      241 ggcgatccgc cgcaggatct ccacgcgcag cagggcgttg gcgaggcggc gtacgagttc
      301 gtcttcttcg ggcggaaccc gaaccttcag ccgcgcgcct acttcgatcc cgggaccggc
      361 acggtcacct actccgcggc gaccgacgcg atggccgcgc cgtcctcgta ctagcgacga
      421 cagcacccgg caaatgtgct attacctgat cattggtaag cgatcggcgg agtgttgtcg
      481 ccgatgttca gagagagggt ggaagccacc atgccggatc aggatgcgcg cgtcgaagag
      541 gtccgcgaac tacggaaggt tcgccagaat cgcgagtaca caccggagct cgtttccgac
      601 gaggatctga acgaattgct cgaggtcgcc cgctggacgg ggagttcgca gaacacccag
      661 ccctggcact tcatcgttgt ccgcgacaag gcctcgctca aggcgatcgg cgattgccgc
      721 ccgaacatcg cctgggttgc cgattcaccg ctcgcgatcg ccctcgtgct caacgggacg
      781 aatcccccga gcgagtacta cgacgagggc cggatcacgg agcgcctgct gattgcggcg
      841 cgctatctcg gactcggcgc cggcaccgcc tggctcggcg aggagccgca gcagacgacg
      901 atcaagcagg ttctcggcat tccccaggaa cacattctcc gctccgtcgt cgtgatcggg
      961 cacccacaac ccggcgccac ccacaagctc ggacgcacca cctccggacg caagccgttg
     1021 gatgaggtgg tcagcttcga gacctttgac gagcatggga actaggccat ttgcgtccct
     1081 ccacgacaac gaaagagaag agaccgagat gcctgagtac gattctcgcg agatttccag
     1141 cttcctgcgc gggctccgtc agcgccgtcg cttcaccgat gagatcatcc cctacgaggt
     1201 gatcgacgat atcctcgagg tcgcccgctg gaccgggagt gccaagaaca accagccgtg
     1261 ggacctcgtc gtggtgcgcg atcgcgacac gctcgcccag ctcgccgagt ccggcacgtt
     1321 cgccggattc ctccgcggcg tcgatgtcgc gatcgtgatc gcgttgaacg ccggtggcgt
     1381 cggtcagctg tacgacgaag ggcgcctgag cgagcggatc atgctgatcg cggaagccta
     1441 cggcctcggc agtggcacgg gatggttcag cgaggagcca ggccagacgc gggtcaagga
     1501 actcctcggg atcccacagg atcgtttcgt tcactccgcg gtcggactcg ggtacatcga
     1561 cacgagtgtg gacgatcgcc cgaacgtcac gctgggacga cgtccgctct ccgacgtcgt
     1621 cagttacggt cgcttcggtc agcacgaagc ctgatcgtct ccgtcgccgg gacgtcgtct
     1681 ctgccgtccc ggcgacccat gtggtcgcct cgccgatatt acaggcgact cgccgggccc
     1741 ggaccacggc tggagtccca ctttggcggt tcgtcgggcc gaccaatcca gatcgcgcgt
     1801 gacaagatcg gcgaggcagg tcatcatagg aggggatgtt ccttacgcca ccgttttctc
     1861 atccctcgaa cgcgatcccg cgctccgcat tcctgcacgc caccggtctg gacctttctt
     1921 ttggattttg acgtcctcca aaaactcttc agcattgtca tcgtcaacct cgtgctctcc
     1981 ggggacaacg ccgtggtcat cgcgatggcc gcgcgcaggc ttcccgagga gaatcggcgg
     2041 aaggccatcc tgtggggcgg cgcgggcgcg atcgtcctcc ggatcatcct gaccgccatc
     2101 gccgcgctcc tgctggacat cccgttgctg caggcgatcg gcggcatcct gctcgtctat
     2161 atctcgatcc ggctgatcac accggagccc gagcatcacg atgccgaggt gaagcaaccc
     2221 ggctcgatcg ggcaggcggt ccagacgatc ctcgtcgccg acctcgtgat gagcctcgac
     2281 aacatcatcg ccgtcggcgg tacggcggaa ggtgagcttg gcctgctcct gatcggtctc
     2341 gtgctctcga tcgcgctgat cctggtcggc agcagcctcg tcgcgaaact gctcgagcag
     2401 tatccgtggc tgctgatcct tggcgttctg gtgctgctcc acgcggcggt caagatgttc
     2461 cttcacgatc atttcgtcga gaagctcttc ggttaccatc acgcaccgga gtgggtctcg
     2521 ctggcgatcg cgccggtgct gaccgtgatc gtcctcgtct ttgtccgcgc ctggcgtggc
     2581 ggcatcgctg gcttcaccga tcgcgaccga gcggccgtga cggcggcacg tgcgaacgat
     2641 ccctccacca cccgcgcgta ggacggatgg cgtactcgat cgcgaccgat cgctgatgcc
     2701 gcggtcgtcc gtaggggcga gcctcgtgct cgccctggcc gtcccttgca cgatctcttt
     2761 ggaacgacac gctcccattc ccgtccccac ggcgcttgtc gccgttctcc ggaacgcgtt
     2821 ttgcggtgga tgtttccgtt ggagtccgcc tgcagcggag ggcgagcacc aggctcgcgc
     2881 ctacgaacgc ggcgattgtg cgacgggctc tgcgcagcag ctgtccgggg agcccgtgga
     2941 tgccaggcgg tccctcgaca ggctcgggag gacggttggg ggctcgggag gacgtggatg
     3001 ggaaggcgtc gcggtggagg caggcggtga cccggatcgg ggagggcagc acggcccgca
     3061 cggcggggcg tggtcttgac acagtttagt cctaaacgta tagtagcttc tgttcagtaa
     3121 gcaatgcggc gtcggtgtga tgccgctggg gacggatcac aacggcaaag gacctttcca
     3181 tgaccgatac cacgaccaca acgtctacct ggaatatcga caaggcgcat agctcggtcg
     3241 agttcgccgt caagcacatg atgttctcga cggtcaaggg ccgcttcggc gagttcagcg
     3301 gcgtcatcac gttcgacacc cagaacctcg cc
//
LOCUS       DASTMD010000678         1537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2740610, whole genome shotgun sequence.
ACCESSION   DASTMD010000678 DASTMD010000000
VERSION     DASTMD010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740610"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1537)
                     /locus_tag="VFQ54_10020"
     CDS             complement(<1..>1537)
                     /locus_tag="VFQ54_10020"
                     /inference="COORDINATES: protein motif:HMM:NF023454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HEU0165369.1"
                     /translation="ALERAQALTSVKIATREQRDEIAEAIGDFRFTTNFGRSLSRLIR
                     SGIGVHHAGMLPKYRRLVEQLAQQGLLRVICGTDTLGVGINVPIRTVLLTALTKFDGA
                     KMRQLNAREFHQIAGRAGRAGFDTAGTVVVQAPEHETENARLLAKAGDDPKKVKRIVR
                     KRAPDGFVSWGLASFERLVAAQPETLVSSMRVSHAMVLNVIGRGGKPFDELRELIENN
                     HEPRPRQRELARQALAIYRTLRTAEIVQQSASGAITLTVDLQPNFALNQPLSPFALAV
                     FDLIQPHPVAEPVGTSSTGDPGAGSAASANGISLALDLISVLEATLDDPRPVLSAQQF
                     AARGEAVAAMKSEGIEYEERMELLEEITWPKPLEELLGAAFETYRASQPWVGDFELHP
                     KSVVRDLYERAMTFNDYIGFYKLARSEGLVLRYLSDAYRAARQTIPDDLKSEELLDLI
                     EWLGELVRQVDSSLIDEWEQLVNPAAVPDLDAPVLPPVPHGVTTNARAFRTLVRNELF
                     RRVQ"
BASE COUNT          250 a          502 c          511 g          274 t
ORIGIN      
        1 ctgcacgcga cggaagagct cgttgcgaac gagcgtgcgg aacgcccgcg cattggtcgt
       61 gacaccgtgc gggacaggcg gcagaaccgg cgcatcgagg tctggcacgg ccgcgggatt
      121 caccagttgt tcccactcgt cgatgaggct cgagtcgacc tgccgcacga gttcgccgag
      181 ccactcgatg agatcgagaa gttcctcgga cttgagatcg tccgggatcg tctgccgcgc
      241 cgcgcgatag gcgtcggaga gatagcgcag cacgagtccc tccgagcggg ccagcttgta
      301 gaacccgatg tagtcgttga acgtcatcgc gcgctcgtag aggtcccgga cgaccgattt
      361 cggatgcagc tcgaagtccc ctacccaggg ctgggacgcc cgataggttt cgaaagcggc
      421 tccgagcagc tcctcgagcg gcttcggcca ggtgatctct tcgagcagtt ccatgcgctc
      481 ctcgtattcg atcccctcgc ttttcatggc ggcgacggcc tctccccgcg ccgcgaactg
      541 ctgcgcggag agcacgggcc gcggatcgtc cagggtggct tcgagcaccg agatgagatc
      601 gagcgcgagc gaaataccgt tggccgaggc cgccgagccc gcaccggggt cacccgtcga
      661 tgaggtcccg acgggctcag ccaccggatg cggctggatc aggtcgaaga ccgcgagcgc
      721 aaacggggac aacggctggt tgagcgcgaa attcggctgc agatcgaccg tgagcgttat
      781 cgcgccgctc gcagactgct gcacgatctc cgccgttctg agggtgcggt agatcgcgag
      841 agcctgacgc gcgagttcgc gctgccttgg ccgcggttcg tggttgttct cgatgagctc
      901 ccgcagctcg tcgaagggct ttcccccgcg tccgatcacg ttgagcacca tcgcgtggct
      961 cacccgcatg ctcgagacga gcgtctcggg ctgagcggcc accagccttt cgaaactggc
     1021 gagcccccag gagacaaagc cgtcaggagc gcgcttccgc actatccgct tgaccttctt
     1081 cggatcgtcg cccgccttcg cgagcagtct ggcgttctcc gtctcgtgct ccggtgcctg
     1141 cacgacgacg gtgcccgccg tatcgaagcc ggctcggccg gcccgaccgg cgatctggtg
     1201 aaactcccgt gcgttcaact gccgcatctt ggcgccgtcg aacttggtca gcgcggtgag
     1261 cagcaccgtt cggatcggca cgttgattcc gaccccgagt gtgtcggtgc cgcagatgac
     1321 gcggagcagc ccttgctggg cgagctgctc gacgagcctg cggtatttcg gcagcatccc
     1381 cgcgtgatgc acgccgatgc cggaacggat gagacggctg agcgagcgcc cgaaattggt
     1441 cgtgaagcgg aaatcaccga tggcctccgc tatctcgtca cgctgctcgc gggtggcgat
     1501 cttgacgctg gtgagcgcct gtgctcgctc gagcgcg
//
LOCUS       DASTMD010000679         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1670978, whole genome shotgun sequence.
ACCESSION   DASTMD010000679 DASTMD010000000
VERSION     DASTMD010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670978"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VFQ54_10025"
     CDS             complement(<1..526)
                     /locus_tag="VFQ54_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU0165370.1"
                     /translation="MPKQVGIDLGTANVLVFLRGRGIVINEPSVVAISAKDGKVKAVG
                     LEARNMLGREPRDTIEVVRPMRDGVIADYIVTQKMLEYFINKAVGRFSLMRPDVMICV
                     PAGVTGVERRAVRDAALKAGARHAYLISEPLAAAIGARVPVADPSGNMIIDIGGGTTE
                     VAVISLNGIVVARSL"
     gene            complement(694..1482)
                     /locus_tag="VFQ54_10030"
     CDS             complement(694..1482)
                     /locus_tag="VFQ54_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0165371.1"
                     /translation="MDLSRVGFGIAGALGTDLIRELAPKVEAAGFHTLWINDTPTGDS
                     LAMAAAAAAVTSHIRFGTGVIPIDRKPPAEIVRQIVDLEIPVHRLVLGIGSGRATTGA
                     LGLVRDSITAIKQDLGVQVVVGALGPRMRRVGAQDGDGILLNWLTPKAALEARQALKR
                     DSDEVGKTDSRLITYVRTAYGEASVKRLAIEAANYGQNPFYAANFVRQGADGIQASVS
                     GDDGETLRAGLTAYLDAVDEVVVRAVTPDDTIAQLEVLLEQIAG"
     gene            complement(1503..2225)
                     /gene="radC"
                     /locus_tag="VFQ54_10035"
     CDS             complement(1503..2225)
                     /gene="radC"
                     /locus_tag="VFQ54_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU0165372.1"
                     /translation="MSDGEARYHLMIRDIHPDERPRERLLNYGAASLSTTELLAIILN
                     TGIQGESVTSIAQRLLSEHGGLSGLLKLDLAELARQRGVGPVKATKLKASLEIGRRLA
                     AMAPEERARVESPEDVVRLVGVEMSVLEQEQLRVLLLDTKHAVIAARTIYQGSANQAT
                     VRVGELFRDAVRHTAVAIVLVHNHPSGDPTPSGADVAMTTDVVAAGKLLDIAILDHII
                     IGQGRHTSLKRLGLGFPPESNR"
     gene            complement(2235..>2673)
                     /locus_tag="VFQ54_10040"
     CDS             complement(2235..>2673)
                     /locus_tag="VFQ54_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-opening dioxygenase subunit LigB"
                     /protein_id="HEU0165373.1"
                     /translation="LGGVPEDDHGPQVVLITPSRAMGRQAMVDFGRVLGEAFRDDPRK
                     IGFIASCDWGHAHTADGPYGFHPDAAKVDAKVVDAVEKNELLSLIELTDEEVQNAAID
                     GLWQTLILAGIQQITPLDSNLISYEHPTYFGMIVAGFSPAAQA"
BASE COUNT          477 a          876 c          840 g          480 t
ORIGIN      
        1 gtagcgaccg cgcgacgacg ataccgttca gcgagatcac ggcgacttca gtggtgccgc
       61 ctccgatatc gatgatcatg ttcccggacg gatcggcgac gggaacccgt gcgccaatcg
      121 ccgcggccag cggctcgctg atcaggtagg catgacgggc cccggccttg agcgccgcgt
      181 cacggacggc tcgacgctcg accccggtca caccggccgg cacgcagatc atgacgtcgg
      241 gacgcatcag cgagaaccga ccgacggcct tgttgatgaa atattcgagc atcttctgcg
      301 tgacgatgta gtcggcgatc acaccgtccc gcatcggcct cacgacctcg atcgtgtcac
      361 gaggttcgcg tccaagcatg ttgcgagcct cgaggccgac cgccttgact ttgccatcct
      421 tcgccgagat agcgacaacg gacggctcgt tgatcacgat tccccgtccg cgcaaaaaaa
      481 cgagcacgtt agccgtgccg agatcaatcc ccacctgttt tggcacgcaa gacctctccg
      541 attcgggatc ggcgcacaga acaccttcag ctcgatacac cgggaagcgg gaaacgagct
      601 tcgcttcccc tgaagatcct cgattctaac acttccggac ggctcgccgg tccggaagtg
      661 gcgtgacttt atgtcacaca gacgagcgag cgactatccc gcgatctgtt ccagcaggac
      721 ttccagctgc gcgatcgtat cgtccggtgt caccgcccgc acgacgactt cgtcgaccgc
      781 gtcgagatac gcggtgagac cggcgcgaag tgtctcgcca tcgtcgccag agaccgacgc
      841 ctggatcccg tccgcaccct ggcgcacgaa gttcgcggcg tagaaggggt tctggccgta
      901 gtttgcagcc tcgatcgcca accgcttcac cgacgcctcg ccgtaggcgg tgcgaacgta
      961 ggtgatcagg cgcgagtccg tctttccgac ctcgtccgag tcccgcttca gtgcctggcg
     1021 cgcctcgagt gcggccttcg gtgtcagcca gttgaggaga atcccgtcgc catcctgcgc
     1081 gccgacccgg cgcatcctgg gaccgagtgc gccgacgacg acctggacac cgagatcctg
     1141 tttgatcgcg gtgatactgt cgcggacgag cccgagcgcg ccggtggtgg cgcggccact
     1201 gccgatgccg aggaccagcc ggtggacagg aatctcgagg tcaacgatct ggcgcacgat
     1261 ctcggcggga ggcttgcgat cgatcgggat gacgcccgtc ccgaaccgga tgtgggaggt
     1321 taccgccgcg gcagccgccg ccatcgccag cgagtcgccg gtcggcgtgt cattgatcca
     1381 gagcgtgtga aagcctgcgg cctcgacctt cggcgcgagt tcgcgaatga gatcggttcc
     1441 cagcgcgccc gcgattccga agccaactcg tgataggtcc atggagtttc tccgatcctg
     1501 acttagcgat tgctctccgg aggaaagccg agcccaagtc gcttgagtga cgtgtgccgt
     1561 ccctgcccga tgatgatgtg atcgaggatc gcgatatcga gcagctttcc ggcggcgacc
     1621 acgtcggtcg tcatcgcgac atcggctccc gatggcgttg gatctccgga gggatgattg
     1681 tgaacgagaa cgatcgcgac cgccgtgtgc cggaccgcat cgcgaaagag ctcgccaacc
     1741 cgaaccgtcg cctggttcgc acttccctga tagatcgtcc gcgcggcgat caccgcgtgt
     1801 ttggtatcga gcaggagcac gcgtagttgt tcctgctcga gcacgctcat ctcgacgccg
     1861 acgaggcgaa cgacgtcctc cggtgattcg acgcgcgccc gttcctccgg cgccatcgcc
     1921 gcgagccgcc gcccgatctc gagcgacgcc ttcagcttcg tcgccttgac gggaccgaca
     1981 ccccgctggc gagccaattc ggccaggtcc agcttgagca ggccgctgag gccgccatgc
     2041 tccgagagga gtcgctgcgc gatcgacgtg acggattcgc cctggatgcc ggtgttgagg
     2101 atgatcgcga gcagttcggt cgtgctgagc gaggccgcgc catagttcaa caaccgctca
     2161 cgcggtcgtt catcgggatg gatgtcccgg atcatcaggt gatagcgggc ctcgccgtcg
     2221 ctcacaagag gcgcctacgc ctgcgcggcc ggcgagaacc cagcgacgat catgccgaag
     2281 taggtcggat gctcgtagga gatcagatta ctgtccagcg gcgtgatctg ctggattccc
     2341 gcgagaatca gggtctgcca cagaccgtcg atagccgcgt tctggacctc ttcgtcagtc
     2401 aactcgatca gcgagagaag ttcgttcttc tcgacggcgt cgaccacctt cgcatcgacc
     2461 ttggcggcat cgggatggaa tccgtacggg ccgtcggctg tgtgggcgtg accccagtcg
     2521 caggacgcga tgaagccgat cttgcgcgga tcatcgcgga atgcctcacc caggacgcga
     2581 ccaaaatcaa ccatcgcctg gcgacccatg gctcggctcg gggtgatcag cacgacctgt
     2641 ggaccgtggt cgtcctccgg gacgccgccg agg
//
LOCUS       DASTMD010000680         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1671135, whole genome shotgun sequence.
ACCESSION   DASTMD010000680 DASTMD010000000
VERSION     DASTMD010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671135"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            708..2078
                     /locus_tag="VFQ54_10045"
     CDS             708..2078
                     /locus_tag="VFQ54_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEU0165374.1"
                     /translation="MSDQLLEVFTLEDSASMPSGTPDVAGITHMRAVSIGASSGAFYP
                     HTPTEFVPAMAAGFGIRAIELMLQTASEYQPEFIDELASNARSAGVRVHAIHTATALH
                     PMFDPYARRAADGRALFQQGIEAAAKLGARALVWHGARRQDVATDEGWERFITLTTEL
                     AHACGEAGVTLAIENVSWCALAQVRHVVQFATRLNEIGGPEQIGFVFDPFQAVEAGAN
                     PFMMLAAMGNRIVDVHISDYREQDAAARHLVPGDGDLPWSALIRAIAGSGYSGPMMIE
                     GPLGTDAIAIGRVRDRLNPLIRSVFDFEPDAGRYETGAAPREDADPGDREADSVTLIS
                     TTTPPAGILHGIELFNARQFYEQHEAIEHEWHAERGPIRRLYQGILQIGVGFHHALNG
                     NQPGAVRLLTDGITKTSEFAPHALGIETGQLIEEARVCLDRLIALGPDRIAEFDPATI
                     PTIVFS"
     gene            2445..>2745
                     /locus_tag="VFQ54_10050"
     CDS             2445..>2745
                     /locus_tag="VFQ54_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165375.1"
                     /translation="MAKRTIRWLATILVATGALAFWAPGVSAHSANVTTSVDCFNYYV
                     TISWDYLDQHPTVSGYTGDYPGNSAGTITVPWGGATSAGGTVSFTWTRDNYQASSN"
BASE COUNT          484 a          911 c          838 g          512 t
ORIGIN      
        1 ggcgtacgaa cgcgatgccc acgcatctcg ggcgccatta cgtagctccg gacaggatcg
       61 tcgccaggtt ggatcggcca acggtcgcct catcttatgt cgttatccat tcccgagcac
      121 catacgtgcc gatgtcatcc ggctgtgtga cctttgggaa catctgccgt gaggggcttt
      181 cgtggtggta gcatcgttgc acacccggga cggcctggcc gcgcccggga ggggaggggg
      241 ctcgggatcg ggcaaccgga tgacccagga cgagtggcct ggctggctct cgtcagtgat
      301 gtccgtcgtg acggcagtgt ggttctgcgc ggcgatgtac ggcatccagg ttgggttccg
      361 gttcggtcgg atccgccaca ccgatgatcg gcaagctcca cgatcgcgtc attccgctcg
      421 acgacgtcga cggttccggc cgatcgaacc atggatggat ggggagcttc cggtcgtcac
      481 cctcaccacg tgaacgctga cacacgcatc gcccgccgcg gtcgatcggt gcaggcgacg
      541 gaacgggagt cgatcctgac gtcgctttgc cgcctgattc cgcgtttgtt ttggaggata
      601 agccgcgcgt ggataccccg caggaaccat ccggaggacg gatcgttccg tttccacagc
      661 aaccagcatc ccaggagccg tcatcgccgg cgcggggcac tcccccgttg tcggatcagc
      721 tgctcgaggt cttcacgctc gaggactccg catcgatgcc ctcagggact ccggacgttg
      781 ccgggatcac ccacatgcgc gcggtgagca tcggcgcctc cagtggcgcc ttctatcccc
      841 acaccccgac cgagttcgtt cccgcgatgg cggctggatt cggtatccgc gcgatcgaac
      901 tgatgctgca gaccgcgagc gagtatcagc ccgaattcat cgacgaactc gcttccaatg
      961 cccgctccgc cggtgtccgc gttcacgcga tccacacggc aaccgcgctc cacccgatgt
     1021 tcgatcccta cgcccggcgc gccgccgatg gccgcgcgct cttccagcaa gggatcgagg
     1081 ccgcggcgaa gctcggggcg agggccctcg tctggcacgg ggcgagacgc caggacgtcg
     1141 caaccgatga gggatgggag cgcttcatca cgcttacgac cgagctcgcc catgcctgtg
     1201 gtgaggctgg cgtcaccctc gcgatcgaga acgtctcctg gtgtgcgctg gcgcaggttc
     1261 gccacgtcgt ccagttcgcg acccgcctga acgagatcgg cggcccggag cagatcggct
     1321 tcgtcttcga tcccttccag gcggtcgagg ccggcgccaa ccccttcatg atgctggcgg
     1381 cgatgggcaa tcgcatcgtc gatgtgcaca tcagcgacta ccgcgagcag gatgccgcgg
     1441 cccgtcacct cgtccccggc gatggcgatc tcccgtggtc ggccctcatc cgcgcgatcg
     1501 ccggcagcgg ctacagcggc ccgatgatga tcgagggacc gttggggacg gatgcgatcg
     1561 cgatcggccg tgtccgcgat cgcctgaatc cgctgatccg ctccgtcttc gacttcgagc
     1621 cggatgccgg tcggtacgag acaggggccg cgccgcgcga agacgccgat cccggcgatc
     1681 gcgaggcgga ttcggtgaca ctcatcagca cgacgacccc gccggcgggc atcctgcatg
     1741 ggatcgagct cttcaacgcc cgccagttct acgaacagca cgaggcgatc gagcacgagt
     1801 ggcatgccga gcgtggtccg atccggcgtc tctaccaggg aatcctccag attggcgtcg
     1861 gcttccacca cgcgttgaac ggcaatcagc cgggagcggt gcgcctcctg acagacggca
     1921 tcacgaagac ctccgagttc gcgccccatg cgctgggaat cgagaccggt caactgatcg
     1981 aggaggctcg cgtctgcctc gatcgcctga tcgcactcgg acccgatcgc atcgccgaat
     2041 tcgaccccgc gacgatcccg acgatcgtct tctcctagca cctctcacat cccgcgaaga
     2101 gggatgaacc caccatccga ggcatgagac ggacggtcac tcaccgcccg aacctgcctg
     2161 cccgcagagc ggccgtgtga ccagatcgca cggccgtatt tgcgtgccag gaccccaccc
     2221 acgacgtatg tccatgctat tcaccatcag gtctacgcaa taattctgta taggctatta
     2281 ctttcagcat gtatccacta cgatagtggg acctttagga gaaattgatc ctgccatgcc
     2341 attgtgtcac tagtactatt agcctacaat cgctgtaacg aagagttgcg tccttgggga
     2401 ggtgtcctac cacgccccac cgcattaacg ttcggggagg tttcatggcc aagcgtacga
     2461 tcaggtggct cgccacaatt cttgtagcaa caggagccct ggctttctgg gctccaggtg
     2521 tcagcgccca ttcggcgaat gtcactacga gcgtcgattg cttcaactac tacgtcacga
     2581 tctcctggga ctatctggac cagcacccga cggtttccgg ctacaccggg gactatcccg
     2641 ggaattccgc ggggacgatc acggttccct ggggcggcgc tacatccgct ggtggtaccg
     2701 tctctttcac gtggacgaga gacaactatc aggccagctc gaact
//
LOCUS       DASTMD010000681         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1758886, whole genome shotgun sequence.
ACCESSION   DASTMD010000681 DASTMD010000000
VERSION     DASTMD010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758886"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFQ54_10055"
     CDS             complement(<1..477)
                     /locus_tag="VFQ54_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165376.1"
                     /translation="MITNPWAAPGRFYKGNLHCHSTLSDGLLPLDEVVSRYRAQGYDF
                     LSVTEHNMIADTSMYRDEHFTTLFGTEIDAPMRPGSYVRLINAVGLPLDFPIDRTLTL
                     PEIARQARAAGAFVAMACPAWCGTTSQDAIDVDALHAVETYSVTCAAGNDRGSSWHL"
     gene            complement(514..1962)
                     /locus_tag="VFQ54_10060"
     CDS             complement(514..1962)
                     /locus_tag="VFQ54_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0165377.1"
                     /translation="MGNELDEQRLTSTSLIPSLHPRGAGQTFVWYGDCCSGVPGADNE
                     RHFAAVNAVFQRLATQPDVVLFPGDNIAGNPGVSAEDTRAMWRYWLDREMAWFYDLDI
                     PIYHTTSNHNTGDAVAEAVFREILTDIPQNGPDDQKGLSYWVRRDDFLYVFVNTNFSG
                     LGGSGHVEHKWLDRVLAENADATYKLVIGHHPIFPTNGYDMHPGWHVDPDQGAAFWDV
                     LVRHGVIAYLCSHILAFDAQVHDGILQVMTGGAVVGVMPEAFEYAHLIQTAIDETGIR
                     YQVLDTEGVAREWLQWPEPALDAPLAPLDIANPPVTPAPLAGDDESAAAWIARFRISG
                     GLTAADHEQTLLTVWTAMDGPEGLWIGTAGPNNRLTVQLVPQQGRGAQRWEGPELAAD
                     QPFDLEIAIHSGMGPGGVLWRPAGGAWNSLSHSTAEGAARFTWPETWQLGHSQSGESD
                     RAFAGSGLAIQYRFDTVSRADLFASHPVVVTE"
BASE COUNT          393 a          643 c          794 g          410 t
ORIGIN      
        1 gaggtgccag ctggaaccgc ggtcgttccc ggccgcacac gttacgctgt aggtttcgac
       61 cgcgtgcagc gcgtcgacgt cgatcgcgtc ctgcgatgtc gtgccgcacc aggcgggaca
      121 ggccatcgcc acgaaggcgc ctgccgcacg cgcctgccgc gcgatctcgg gaagggtcag
      181 ggtgcggtcg atcgggaagt cgagcggaag accgacggcg ttgatgagtc gcacgtaaga
      241 acccgggcgc atcggggcat cgatctcggt gccgaacaac gtggtgaagt gctcgtcgcg
      301 atacatgctc gtatcggcga tcatgttgtg ctcggtgacg gagaggaagt cgtagccttg
      361 cgcgcgatac ctgctcacga cctcgtcgag tggcaggagg ccatccgaga gcgtggagtg
      421 gcaatggaga ttgcccttgt agaagcgacc gggtgcggcc cagggattcg tgatcatgtt
      481 cgttgctccg gcgatctggg tgggtcgtac ccgctactcg gtcacgacga ccgggtgcga
      541 tgcgaagagg tcggcgcgtg acacggtgtc gaaccggtac tggatggcga ggccggagcc
      601 ggcgaaggcg cgatcggatt caccgctctg gctgtgaccg agctgccagg tctccggcca
      661 cgtgaagcgg gccgcgccct cggcggtcga gtggctgagc gagttccagg cgccgccagc
      721 cgggcgccag agcaccccgc ccggacccat gccgctgtga atcgcgattt cgagatcgaa
      781 gggttggtct gccgcgagtt cgggaccttc ccagcgctgc gcgccgcgcc cttgctgggg
      841 cacgagttgc accgtgagac gattgttcgg accagcggtg ccgatccaca gcccctcggg
      901 accgtccatc gccgtccaga cggtaaggag ggtctgctcg tgatcggccg ccgtgaggcc
      961 gccggagatg cggaagcggg cgatccaggc cgcggcggat tcatcgtcgc cggcgagggg
     1021 tgccggtgtg accggcgggt tggcgatgtc gagcggagcg agtggtgcgt cgagggcggg
     1081 ttccggccac tgcagccact cgcgggcgac accttcggtg tcgagcacct gataccggat
     1141 gcccgtctcg tcgatcgcgg tctggatcag gtgggcgtat tcgaaggctt cgggcattac
     1201 accgacgacg gcgccgccgg tcatcacctg caggatgcca tcgtgaacct gggcatcgaa
     1261 ggcgaggata tgactgcaga gataggcaat cacgccgtga cgaacgagga catcccagaa
     1321 tgcggcgccc tggtccgggt cgacgtgcca accgggatgc atgtcatagc cgttggtcgg
     1381 aaagatcgga tggtggccga tgaccagctt gtatgtcgca tcggcgttct ccgccaggac
     1441 gcgatcgagc cacttgtgct cgacgtggcc cgagccaccg agcccggaga agttggtgtt
     1501 gacgaagacg tagaggaagt catcgcggcg gacccagtag gagagcccct tctgatcgtc
     1561 cgggccgttc tgcgggatat ccgtgaggat ttcgcggaag accgcttcgg ccacggcatc
     1621 gccggtgttg tgattgctcg tcgtgtgata gatcgggatg tcgaggtcgt agaaccacgc
     1681 catttcacgg tcgagccagt atcgccacat cgcgcgggtg tcttcggcgc tgacgccggg
     1741 attgccggcg atgttgtcgc cggggaagag gacgacatcc ggctgggtcg cgagccgttg
     1801 aaagacggcg ttcacggcgg cgaagtggcg ctcgttgtcc gcaccgggaa caccggagca
     1861 gcagtcgccg taccagacga aggtctgacc cgcaccgcgc gggtgcaggc tcgggatcaa
     1921 actcgtgctt gtcagtcttt gctcgtccaa ttcgttcccc atgctagatt gtttccggaa
     1981 cagttcgcgt atccggatcg gcaattgatg acaacgagca accgggtcaa ggcgtgcgtt
     2041 gttgctgaac gtcggtccga agcgatggtc ggtgcagtcg gctactatca ggcgctggtg
     2101 gactgctgtt ccatcacgct gaaagaatac ggacattttt cccggcgatt gtgatcaaaa
     2161 ggtaaaaaga tccgtatatc gaccgatcgc ggtcatgaat atccatgggg cgtgcgtatc
     2221 cgaggggagt ggcgtcaggt
//
LOCUS       DASTMD010000682         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_867224, whole genome shotgun sequence.
ACCESSION   DASTMD010000682 DASTMD010000000
VERSION     DASTMD010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867224"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(524..1795)
                     /locus_tag="VFQ54_10065"
     CDS             complement(524..1795)
                     /locus_tag="VFQ54_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEU0165378.1"
                     /translation="MLGRDVVATTCELITFYNAVREHPTRSSQDLEVTVMVQDHDAQA
                     AFGHERKNRQQDKIRVAIIGVGNCASSLVQGVQYYQNAPEDEFVPGLMHVNLGGYHIR
                     DVEFSAAFDIDANKVGKDLSEAIWAEPNNTIKFSDVPHLGVPVHRGMTHDGLGRYLSE
                     IITKADGPTANIVQILRDTNTDVVVSYLPVGSEMATKWYVEQILEAGCAFVNCIPVFI
                     AGTEYWNQRFLEKGLPIVGDDIKSQVGATITHRMLTRLFADRGVRIDRTYQLNFGGNT
                     DFLNMLERDRLESKKISKTNAVTSQLDYPVAPENVHVGPSDHVPWLKDRKWAQIRLEG
                     TTFGDVPLSVEMKLEVWDSPNSAGVVIDAIRCAKLALNNGIGGALLAPSSYFMKSPPE
                     QYHDDIARDRVEEFIEIYKRKPAGVEAPAAD"
BASE COUNT          436 a          732 c          774 g          455 t
ORIGIN      
        1 aagctcttaa cttttctgaa gccactgtaa tcgggaaagc ttcgggcgtc aagcacaatt
       61 ctcgcaagaa aatctggagg tcctgccgcg ggcgcagcga gggtcagacg cggggattac
      121 gccggatgcg ggtcggcggt gccgcattga cccggcggcg accacatggc tcgccgggac
      181 gggacctccg ggtctcgccc ccgcggcggc gctcggggca ggcgcccgaa cggacgccgg
      241 cggcaacgcg gtcgcgcgtc cgagggcaca tctctgtccc tcatggcgaa atccggaacc
      301 gtcgacacgt ttgacgctgc cggggtcggt agggtggggt tttatacccc gccgcgacgc
      361 ggattcggcg gaatctcccg gtgcgacgcg ctccggaagc ggatcggcta cgccgcattg
      421 acccggcggc gaccacatgg gtcgccggga cgggaacgac acgcgcaccg gaggtcgggg
      481 ggacagggag gtcccccctc gagcctaccg ggaatcatcg cgtctagtcg gcggctggcg
      541 cctcgacgcc ggctggcttg cgcttgtaga tctcgatgaa ctcctcgacg cgatcgcgtg
      601 cgatgtcatc gtggtactgc tccggcggcg acttcatgaa gtagctcgac ggggcgagca
      661 gtgcgccacc gatgccgttg ttcagggcga gcttcgcgca acggatcgca tcgatcacga
      721 cgccggcgct gttcggggaa tcccagactt ccagcttcat ctcgacgctc agtggcacgt
      781 cgccgaaggt cgtcccctcg aggcggatct gcgcccactt gcggtccttc agccacggca
      841 cgtgatccga aggaccgacg tggacgttct ccggtgcgac cggatagtcg agctgggagg
      901 tgacggcgtt cgtcttcgag atctttttgg actcgaggcg gtcgcgctcg agcatgttca
      961 ggaagtccgt gttgccgccg aagttgagct ggtaggtacg atcgatgcgg acaccacgat
     1021 cggcgaagag gcgcgtcagc atgcggtgcg tgatcgtcgc accgacctgg ctcttgatgt
     1081 cgtcgccaac gatcggcaag cccttctcga ggaagcgctg attccagtat tcggttccgg
     1141 caatgaagac ggggatgcag ttcacgaacg cgcacccggc ctcgaggatc tgctcgacgt
     1201 accacttcgt ggccatctcg gatccgaccg ggaggtagga gaccacgaca tcggtgttcg
     1261 tgtctcgcag gatctggacg atgttcgccg tcgggccgtc ggccttggtg atgatttcgg
     1321 agaggtagcg accgaggccg tcgtgggtca tgccacgatg aacggggaca ccaagatgcg
     1381 gaacatcgga gaacttgatc gtgttgttcg gttcagccca gatcgcttcg gagagatcct
     1441 tgccaacctt gttggcatcg atgtcgaagg cggcactgaa ctccacgtca cgaatgtgat
     1501 agccaccgag attgacatgc atcaggccgg ggacgaactc gtcttccggt gcgttctggt
     1561 aatactgaac accttgcacc agtgacgatg cgcaattccc gacgccgatg atggcgacgc
     1621 gaatcttgtc ttgttgtcta tttttccgct catgcccaaa ggcagcctga gcgtcgtggt
     1681 cctgaaccat gacggtgacc tccagatcct gcgagctccg cgtcgggtgc tcgcgcactg
     1741 cgttgtagaa ggtgatgagc tcgcacgtcg tggccacgac gtcccgcccc agcatctgcg
     1801 gttcctgaag gctcgcgcga attggtgcat ccagcggagc cgcaaatgtg ttgctggcga
     1861 tcatacacga attttctcgt tgtgccaccc cgtcatccgt gcgtccgttc gcgacactga
     1921 ccgtctctgg ccatgttgcc ccgttgatgg cagtggcacg ggggactcgg acgcccggca
     1981 ggatcgatgt gcttgtcgcc cggtccaatt ccgccggact gagcagccgg atagttacga
     2041 gtattccgta tatcaatcat tgcaaagcag gtttacggat gccgccggcg tcatggctcg
     2101 agcacatttc ggcgggttcg cggcgagacg tatcctccaa cgcagtcgca tgcgcactcg
     2161 tcatttcgag cctgtcgaga catccccgtg cgaagcacat ccggtcccgc cggaaaccaa
     2221 caccaacgat cgggaaaggc gctccgccga aaacggatcg gcgctgccga tccgaggcga
     2281 ccacatgggt cgccgggacg ggatgtgccg accgccagcc gcaaaggcaa ctgccgcacc
     2341 cccgagcagt aaactgcgat gaagggtttt caggaacgcc atagacggca gttcccc
//
LOCUS       DASTMD010000683         4471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2473298, whole genome shotgun sequence.
ACCESSION   DASTMD010000683 DASTMD010000000
VERSION     DASTMD010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2473298"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1694)
                     /locus_tag="VFQ54_10070"
     CDS             complement(<1..1694)
                     /locus_tag="VFQ54_10070"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEU0165379.1"
                     /translation="MTWRVGVDAGGTFTDVCFFNEETGELEVAKVSSTPSDPSRAVIA
                     GVRDVLASGAPDKVVTYFAHGSTVATNALIQKRGVETGLITTEGFRDLLELGRQKRPH
                     LYDLQTDKPVPLVTRDRRIEVKERLLHDGTVVSEIDEEEVRAAVRKLRDQGVRSIAIC
                     FLYSFVNGDHERLVERIVQEEYPEAFVTSSYQVMPEFREYERLSTVVVNAYLGPVMAS
                     YLDRLAPDLKDAGIPVHPHINQSNGGVISFETAASMPVRTVLSGPSSGIVGATYVSGL
                     SGHGDLITFDMGGTSTDVSLIRDGRPGLASEADVEGYPVKTPMLDIRAVGAGGGSIAW
                     IDSGGHLKVGPQSAGAVPGPVCYGLGNEEPTVTDANVVLQTLNPTHLLGGRMPIDAAA
                     SAAAIDRLAERLGLDRMPTAQGIIRVVIANMARAIRVISVQRGYDPRDFTLVAFGGAG
                     PLHASRLAKELEIPRVLVPEVPGLLCALGLLVADLRTDYSMTRLLDANESAIADLEEV
                     LGDLAVRADTWFGLEEIPEHRRMLRFAVDMRYRGQNYELPVDIPAGPVTASLIRELR"
     gene            complement(1698..3308)
                     /locus_tag="VFQ54_10075"
     CDS             complement(1698..3308)
                     /locus_tag="VFQ54_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0165380.1"
                     /translation="MTDAQDRRRSSTPGRVGVLSRRRALEMAAVASGAVALGSRQVTA
                     AAQAAATPVTGGTLIYGLSTDPPNLDPRVGSGYAAATVKQQVYSSLIRVNEKFEFLPE
                     LALSWKVATDRLSVTFSLRPNVKWHDGSPFTADDVKFTFDTILAADSTAAAKADLFTI
                     DSVEVVDPLTVVFHLNQPDAMLLAVLQDNTCSIFSKSVVGGGADLTTTLIGTGPFKYV
                     GRETGVSITLARNPDFWDPALPYLDGLVFQPISDDTARVSALQSGQVSLIDYVPQISQ
                     TELESSSDFKLYSDVVFTQMYVLLNQASDPLKKQLVRQAIAYAVDRDAVLQAVFFGRG
                     KTLTGGVIPEIIGGSTKLDGYYTRDLDKAKALLTEAGYPDGIDLTLTVTSTYAFHQRT
                     AEVVQATLGDANIRVKLELLEWATLLDRQNNGPYELMVQGGGFPYPHPAAVGSFVGAK
                     SAVVKAEHYVDPDMDTLVASVRKAPDADSLATLAYQIDERMLEQLPRTFLVGRSQAEA
                     SSSKVSGYTHIPGGSYSGLNIRVTWLEG"
     gene            3734..4255
                     /locus_tag="VFQ54_10080"
     CDS             3734..4255
                     /locus_tag="VFQ54_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU0165381.1"
                     /translation="MDSGSPISGEDMDTRIINLLQEHPRASYTTIARLMGASDTTVRR
                     RMNALFEQGRLRMMALPDPARVGFPFSAIVYIRTTHGGARTIAEKLSSHWQTTYVSEL
                     IGAWSISCIVRERSLEDLAIFLHETVERIPGVEEVEAQVVAKVHKGWGQWRIPEGAAV
                     MPRTPDTEGGAIG"
BASE COUNT          841 a         1389 c         1483 g          758 t
ORIGIN      
        1 cgcaattcgc ggatcagact cgccgtaacg gggccggcgg ggatatcgac cggcagctcg
       61 tagttctggc cccggtagcg catgtcgacc gcgaaccgga gcattcgccg gtgctcggga
      121 atttcctcga ggccaaacca ggtatccgcg cgcaccgcga ggtcgcccag cacttcctcg
      181 agatcggcga tcgcggattc gttggcgtcg agcaaccgtg tcatcgagta gtcagtccgg
      241 agatccgcca cgagcaaccc gagggcgcag agcagacccg gaacttcggg gacgaggacg
      301 cgcgggatct cgagctcctt cgccagacga ctggcgtgga gcggccccgc gccgccaaat
      361 gccaccagcg tgaaatcgcg cggatcgtag ccgcgctgca cggagatcac ccggatcgcg
      421 cgcgccatgt tggcgatcac gacgcggatg atgccctggg ccgtcggcat ccgatccaga
      481 cccaggcgct ccgccagccg gtcgatcgcc gccgcggacg ccgcagcgtc gatcggcatg
      541 cgaccgccga gaagatgcgt ggggttcagc gtctgcagga cgacgttcgc gtcggtgacc
      601 gtcggctcct cgttcccgag gccgtagcag accggccccg gaacggcgcc ggcgctctgc
      661 ggtcccacct tgaggtgacc gccggagtcg atccaagcga tcgagccgcc acccgccccg
      721 accgcgcgga tgtcgagcat cggcgtcttg acgggatagc cctcgacatc ggcttcgctg
      781 gcaagcccgg ggcgtccgtc gcggatcagc gagacgtcgg tggaggtgcc gcccatgtcg
      841 aaggtgatca ggtccccgtg cccggagagc ccggagacgt aggtcgcacc aacgatcccg
      901 gagctgggcc cggagaggac cgtgcgcacc ggcatgctgg ccgcggtctc gaacgagatc
      961 accccgccgt tgctctggtt gatatgcggg tgaaccggaa tgccggcgtc cttgagatcg
     1021 ggtgcgagac gatcgagata gctcgccatc acgggcccga ggtaggcatt caccacgacc
     1081 gtgctgagcc gctcgtactc gcggaactcc ggcatcacct ggtaggaact cgtgacgaat
     1141 gcctccggat attcctcctg gacgatccgc tcgaccagtc gctcgtgatc tccgttcacg
     1201 aagctgtaga ggaagcagat cgcgatcgat cgcaccccct gatcgcgaag cttgcgcacc
     1261 gcggcccgga cttcctcctc atcgatctcg gagacgacgg tgccgtcgtg gagcaggcgt
     1321 tccttcacct cgatccgacg atcgcgggtg acgagcggca cgggcttgtc cgtctgcaga
     1381 tcgtagagat gcggccgctt ctggcgtccc agttcgagga gatcgcgaaa tccttcggtg
     1441 gtgatgaggc cggtctccac cccacgcttc tggatcagcg cattcgtcgc cacggtgctt
     1501 ccgtgcgcga agtaggtgac caccttgtca ggagcaccgc ttgccagcac gtcgcggaca
     1561 ccggcgatca ccgcacgtga cggatcggac ggtgtactcg agaccttcgc gacttccagc
     1621 tcgccggtct cttcgttgaa gaagcagaca tcggtaaacg ttccgccggc atcaacccca
     1681 accctccagg tcatgactta tccctccagc caggtcacac gaatgttcaa tccggaataa
     1741 ctgccaccgg ggatgtgggt gtatccggag accttgctgc ttgatgcttc cgcctgcgac
     1801 cgcccaacga ggaaggtgcg gggaagctgt tcgagcattc gctcgtcgat ctggtaggcg
     1861 agtgtggcaa gcgagtccgc atccggcgcc ttccgcaccg aggcgaccag cgtgtccatg
     1921 tccggatcga cgtagtgctc cgccttgacc acggcgctct tggcaccgac gaaggagcca
     1981 accgccgccg gatggggata ggggaagccg ccaccctgga ccatgagctc gtagggaccg
     2041 ttgttctggc gatccagcag cgtggcccat tcgagcaatt cgagcttgac gcggatgttc
     2101 gcgtcgccca gcgttgcctg gacgacctcc gccgtgcgct ggtggaaggc gtaggtcgag
     2161 gtcacggtca gcgtgagatc gatgccgtcg ggatacccgg cctcggtgag gagcgccttc
     2221 gccttgtcga gatcgcgggt gtaatagccg tccagcttcg tgctgccgcc gatgatctcg
     2281 gggatcaccc cacccgtcag cgtcttgcca cgtccgaaga agaccgcctg caggacggca
     2341 tcgcggtcga cggcgtaggc gatcgcctgg cggacgagtt gcttcttgag cggatccgag
     2401 gcctggttga ggagcacata catctgcgtg aagacgacat cggagtagag cttgaagtcg
     2461 gaactcgact ccagctcggt ctgggagatc tgcgggacgt agtcgatcag gctcacctga
     2521 ccactctgca gggcactgac gcgggcggtg tcatccgaaa tcggctggaa gacgagacca
     2581 tccagatagg gaagcgccgg atcccagaaa tccggattgc gcgccagtgt gatcgagacg
     2641 ccggtttcgc gcccgacgta cttgaacggc ccggtgccga tcagggtggt cgtgaggtcg
     2701 gcgccacccc cgacgacgct cttgctgaag atcgagcagg tgttgtcctg cagtaccgcc
     2761 agcagcatcg cgtccggctg attgagatgg aagacgaccg tgagcggatc gacgacctcg
     2821 acggaatcga tcgtgaagag atccgccttg gccgccgccg tgctgtccgc ggcgaggatc
     2881 gtgtcgaagg tgaacttgac atcatcggcg gtaaacgggc tgccatcgtg ccacttgacg
     2941 ttcggccgca gcgagaaggt taccgagagc cgatcggtgg cgaccttcca cgagagcgcc
     3001 agttcgggga ggaactcgaa tttctcgttg acgcggatga gcgaactgta gacttgctgc
     3061 ttgaccgttg ccgctgcata tccggagccg acccgcggat cgaggttggg aggatcggtg
     3121 ctcaagccat agatcagcgt gccgccggta accggagtcg ccgctgcctg cgcggcggct
     3181 gtcacctgtc gcgagccaag cgccaccgct ccggatgcca ctgccgccat ctcaagggcc
     3241 ctgcggcggg aaaggacgcc aactcgaccg ggagtcgatg aacgccggcg atcttgcgca
     3301 tctgtcatgg aagtctcctc aacattcggt tcctgttgtc agcgaatgga tgaacgatgt
     3361 ccctcgacga agatcgacac ctccagttcg gccatgcgac ggccacgggt acgacaaagc
     3421 ggcccatgct cccggcagcg gcgggcatgc accagcgaat tcacgatccg atggggaaat
     3481 caggacattg cttcgagaag atggggagcg cacgctccgc caccggttgg catgccggtc
     3541 acgcgatcgg gaagaagggg aatgcgggag caacgtggtc ccgccagtcg gctgcgacca
     3601 gagccatcga gggcgtcacg ccaacgatgg acgttcctta aacaaaaggt tagcatcgaa
     3661 tctcgacggc gaaaacgtcg catctgacga aatgtgtcgt gcacatcgaa caaattgaag
     3721 gattacgcca cacatggaca gcggatcgcc gatatccggc gaagacatgg atacacggat
     3781 catcaatctg ctgcaggagc acccgcgcgc gtcctacacg accatcgcgc ggttgatggg
     3841 agcgagtgac acgaccgttc gccgccggat gaacgcgctc ttcgaacagg gtcgactgcg
     3901 catgatggcg cttcccgacc cggcgcgggt ggggttcccg ttctcggcga tcgtctacat
     3961 ccgcacgaca cacggcggcg cacgcacgat cgccgagaag ctgagcagcc actggcagac
     4021 gacctatgtc tcggaactga tcggcgcctg gagcatctca tgcatcgtgc gggagcgatc
     4081 gctcgaggat ctcgcaatct tcctgcacga aaccgtcgag cgaattcccg gggtcgagga
     4141 ggttgaggcg caggtggtgg caaaggtcca caagggctgg ggtcagtggc ggattcccga
     4201 gggcgcggcg gtcatgccga ggacgccgga taccgagggt ggcgccatcg ggtagtcatc
     4261 ttcggcggaa cgaaccgcgg cgagggtcag gactgcccgc gctcaatcaa gaacgagtgc
     4321 cgcgaagagg cgatcgagcg tcgcctgagg atcggcggtg aggccggtgt ggacggggga
     4381 aggctggatc accgtgttgc tgatggacga gatccagtgg aagcgttccg agatgtcgag
     4441 ttgcgcgacg gggccggcat ctttctcgcc c
//
LOCUS       DASTMD010000684         1699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1402590, whole genome shotgun sequence.
ACCESSION   DASTMD010000684 DASTMD010000000
VERSION     DASTMD010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402590"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFQ54_10085"
     CDS             complement(<1..613)
                     /locus_tag="VFQ54_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0165382.1"
                     /translation="MTTLRVALIGGPTYDPLYDRLPDFTARTGIDVEVVAQLPHHDLN
                     AFVATHHAELDLISTHIKYAPSQVDHLLPLDDLLNEGDLAPFLPGPLAQSRINGTLFQ
                     LPRRTDTRLLVYRTDLVARPPATWFDLPDLAADAAKPPGQFGFAFCTQPSGLFGAFYE
                     LLASAGGSLFTDDLRPNLRTNKARWAVQFLKSLHAVSPPESIAW"
     gene            complement(693..>1699)
                     /locus_tag="VFQ54_10090"
     CDS             complement(693..>1699)
                     /locus_tag="VFQ54_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU0165383.1"
                     /translation="YALWDTKLSDYSSVHRRAGRDLVTPYVEACRAAGLKVGFYFSLC
                     DWHHPDYPFELSEDRHVRAESSYTPGSPRTIAEDPARWERYLEFMHGQVRELLTDFGR
                     IDLLWFDGHWEHTPEEWKSAELERMIHELQPHCVVNNRIGSDDLADYVTPEQHVPVTP
                     PDYAWETCLTINETWAYNRHDRAYKSSIELIASLAEITAKGGNLLLNVGPTPDGEIAP
                     ELASRWQVIAEWMKANHEAIHDAGPGLEPGAFYGPSTGNETARYLFLLGPVNGDVARV
                     RGLDRGVRAATILATGQPLEMDHHGGELEGGLLRVTVPASLIDPLVTVVKLHLAQPTR
                     "
BASE COUNT          291 a          506 c          609 g          293 t
ORIGIN      
        1 cccacgcgat cgattccggt ggcgagacgg catggaggct cttcaggaac tgcacggccc
       61 agcgtgcctt gttcgtgcgc aggttcggcc ggagatcgtc ggtgaagagt gagccgccgg
      121 ccgaggcgag gagctcgtag aaggcgccga acagccccga gggttgcgtg cagaaggcga
      181 agccgaactg gcccggcggc ttggccgcgt ctgccgccag atcgggaaga tcgaaccacg
      241 ttgccggcgg tcgggcgacg aggtcggtgc ggtagacgag tagtcgcgta tccgtgcggc
      301 gcgggagctg gaagagcgtg ccgttgatcc gggattgcgc cagcggaccg ggaaggaacg
      361 gcgcgagatc tccttcattg aggagatcgt ccagcggcag caggtggtcc acctgggacg
      421 gcgcgtactt gatatgtgtc gagatgaggt cgagttcggc gtgatgcgtc gcgacgaagg
      481 cgttcaggtc gtggtgaggg agctgggcaa cgacctcgac gtcgatcccg gtccgagccg
      541 tgaaatctgg caggcgatcg tagagcggat cgtaggtcgg gccgccgatc agcgccacgc
      601 ggagtgtcgt cattgttgct cccttccgaa actcaaccga tggtacgtcg ccggtaccgt
      661 atccgagggc cgggtgaacc cgccacctct cgtcaccggg tgggctgtgc gaggtggagc
      721 ttgaccacgg tcacgagggg atcgatgagg gacgccggga ccgtgacgcg aaggagaccg
      781 ccttccagct cgccaccatg atggtccatc tccagtggct gcccggtcgc gaggatcgtc
      841 gccgcgcgca cgccgcgatc gagaccgcgc acgcgggcga cgtcgccgtt caccggaccg
      901 agcaggaaga ggtagcgcgc cgtctcgttt ccggtcgagg gaccgtagaa ggcgcccggt
      961 tcgagacccg gcccggcatc gtggatcgcc tcgtgattcg ctttcatcca ttccgcgatc
     1021 acctgccagc gggaggcgag ttccggcgcg atctcgccgt ccggggttgg cccgacgttg
     1081 aggagcaggt tgccaccctt cgccgtgatc tccgcgagcg aggcaatcag ctcgatgctc
     1141 gacttgtagg cgcgatcgtg cctgttgtac gcccacgtct cgttgatcgt gaggcatgtt
     1201 tcccaggcgt aatccggcgg cgtaaccgga acgtgctgct ccggcgtgac gtagtccgcg
     1261 aggtcgtccg agccgatccg gttgttgacg acgcagtgcg gctgcagctc gtggatcatc
     1321 cgctcgagtt ccgcggactt ccattcctcc ggcgtgtgct cccaatgacc gtcgaaccag
     1381 aggagatcga tccgtccgaa gtcggtcagc agctcgcgaa cctgaccgtg catgaactcg
     1441 agatagcgtt cccagcgtgc cggatcttcg gcgatcgtgc gcggcgagcc gggggtgtag
     1501 ctggattcgg cgcggacgtg gcgatcctcc gagagttcga acggatagtc cgggtgatgc
     1561 cagtcgcaga gcgagaagta gaagccgacc ttgagtccgg cggcgcgaca ggcctcgaca
     1621 taaggggtga cgagatcgcg accggcacga cggtgaaccg aggaatagtc gctcagcttc
     1681 gtatcccaga gcgcgtagc
//
LOCUS       DASTMD010000685         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_330819, whole genome shotgun sequence.
ACCESSION   DASTMD010000685 DASTMD010000000
VERSION     DASTMD010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330819"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFQ54_10095"
     CDS             <1..327
                     /locus_tag="VFQ54_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165384.1"
                     /translation="QWRDWVANGDVLEFVATVGAFILVIVGLRKVPLYYTAFVLPPLI
                     VPLFSPSSVHPLMSMPRFVLPLFPLFVVIALYATNRRVSYALAVISSIGLILLTMQFA
                     QWYWVA"
     gene            478..2151
                     /locus_tag="VFQ54_10100"
     CDS             478..2151
                     /locus_tag="VFQ54_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU0165385.1"
                     /translation="MVEAVADRTELGASAHQQMTGGQALVASPLRQGIDTIFALPGVQ
                     LDGAFDALYDAQQRGDIRVIHPRHEQTTAYMADGYARVTGRLGTCLVVPGPGLLNATA
                     ALSTAYSCNSPVLCVTGQIQSNLIGFGRGQLHEIPNQLEMIRSVTKHSQRALKPEDVP
                     ALVDTAVSTLWQGRVRPVEIEIPPDTLFAKGDIQLLPGVPARDRPAGDPDQIAAAAKL
                     LGAATSPIIFVGGGIHGAEAWDELRELAELLEAPVVMTSNGKGAISSRHHLAHSTLGA
                     SKLLPEADVVLTVGTRFVEPATAPWGLGPNRTVIQMDIDAEEIGRNYPVTVGILADAR
                     AGLAALANEVGKHNRIRESRETELRAVQDWCSETFHRIKPQAGFADAIRQELPEDGIF
                     VGEMTQMGYFGNSAFPVYQPRTYFTPGYQGTLGWGFPTSLGVKIGAPDQVVISVNGDG
                     GFGFALNELATQAHHGIASITLVFNDTAYGNVKRIQTVEFAGRTIASDLTNPDYMKLA
                     GAFGVTGRTATTPAELATQLRESIKANEPTLIEIPVPPMPDVLKELRLR"
     gene            complement(2353..2583)
                     /locus_tag="VFQ54_10105"
     CDS             complement(2353..2583)
                     /locus_tag="VFQ54_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165386.1"
                     /translation="MSEEELYGYLNDLLYEEAGEAAATSGKAIEKELESAGFIAAEAA
                     STYAIKLVLANNAFITRQLLDLGVIGANKGVS"
BASE COUNT          465 a          920 c          837 g          489 t
ORIGIN      
        1 cagtggcgcg actgggttgc gaacggcgat gtccttgagt tcgtcgcgac cgtgggcgca
       61 ttcatcctcg tcatcgtcgg cttgcgcaag gtcccgctct actacacggc gttcgtcctg
      121 ccgccgctga tcgtgccgct cttcagcccg agttcggtcc atccgctgat gtcgatgccg
      181 cgcttcgtgc tcccgctctt ccccctcttc gtggtgatcg cgctctatgc gacgaaccgt
      241 cgtgtcagct atgcgctcgc cgtcatctcg tcgatcggcc tgattctgct cacgatgcag
      301 ttcgcacagt ggtactgggt cgcctgatat cgctgcaccg atccacgcgt ccaacaccgg
      361 gcaatgacgg tacaatcgca gccagaacga cgcgatcgca cagcacgcca cgggccggac
      421 ggcgcgttct tgagctatcg ctcgcaacgt cgcgagcatc atgatgggag aagtgagatg
      481 gtcgaggcgg tcgcggaccg aacggagctt ggcgcttctg cgcatcagca gatgacggga
      541 ggacaggcgc tcgtcgcctc gccgctccgg caggggatcg acacgatctt cgccctcccg
      601 ggcgtccagc tcgatggcgc cttcgatgcg ctctacgacg cccagcagcg cggcgacatt
      661 cgcgtcatcc acccgcgtca cgagcagacg accgcctaca tggcggacgg gtatgcccgg
      721 gtgaccggtc gtctcggcac ctgcctcgtc gtgcctgggc ccggccttct caacgccacc
      781 gcggcactct cgaccgcata ctcctgcaac tcaccggtgc tctgcgtcac cgggcagatc
      841 cagtccaacc tgatcggatt cggacgcggg cagctccacg agattcccaa ccagctggag
      901 atgattcgct cggtcacgaa gcactcccag cgggcactca aaccggaaga cgtaccggcc
      961 ctcgtcgaca ccgccgtcag cacgctctgg caaggccggg tgcgcccggt cgaaatcgag
     1021 atcccgccgg acaccctctt cgcgaaggga gacatccagc ttctcccggg tgtccccgca
     1081 cgggatcgcc ccgcgggcga tcccgaccag atcgccgccg ccgcgaagct cctcggcgcg
     1141 gcaacgtctc cgatcatctt cgtcggcggg ggaattcacg gcgcagaggc ctgggacgaa
     1201 ctccgggaac tcgccgagct cctggaagcg ccggtcgtga tgacgtcgaa cggaaagggc
     1261 gcgatttcat cccgtcacca tctcgcccat tccacactcg gcgcgtcgaa gttgctgccc
     1321 gaggcggacg tcgtgctgac cgtcgggacg cgtttcgtcg agccggcgac cgcaccgtgg
     1381 ggtctcgggc cgaatcggac ggtgatccag atggacatcg acgccgagga gattggccgg
     1441 aactacccgg ttaccgtcgg catcctcgcc gacgccaggg ccggtctcgc cgccctggcg
     1501 aatgaagtcg gcaagcacaa ccgcatccgt gaatcccgcg agacggagtt acgagcggtg
     1561 caggactggt gttccgagac cttccaccgg atcaagccac aggcgggatt cgctgacgcc
     1621 atccgccagg aactgccgga ggatgggata ttcgtcggcg agatgacgca gatggggtac
     1681 ttcgggaact ccgccttccc cgtctaccag ccccggacct acttcacgcc aggatatcag
     1741 gggacgctgg gctggggctt tccgacctcg ctcggcgtca agatcggcgc cccggaccag
     1801 gtcgtgattt ccgtgaatgg cgatggcggc ttcggcttcg cattgaacga gctggcgacg
     1861 caggcgcacc acggcatcgc ctcgatcacg ctcgtcttca acgacaccgc ctatggcaac
     1921 gtgaagcgga tccagacggt ggagttcgcc ggtcgcacca tcgcttcgga tctcacgaat
     1981 cccgactaca tgaagctcgc cggggccttc ggtgtcaccg gccgcacggc gacgacaccg
     2041 gcagaactcg cgacgcaact ccgggagtcg atcaaggcga acgagccgac gctgatcgag
     2101 attccggtgc caccgatgcc ggatgtcctg aaggaactcc ggcttcgctg atagcgaatg
     2161 acgcgaagac accaggatcg aatcgatcct ggtgtcttcg cgtcgcggga gagtacagcg
     2221 caaaccgcaa cgacgaccac gcatgcggcc ggaccggatg cggcgttccg cacggcgttc
     2281 gctcgcaggg cgggaccgcc tctccttcgc acggggatcg cgctccccgt gacgacggtc
     2341 ccgatcgccg ctctagctga cgcccttgtt cgccccgatc acaccgagat cgagaagttg
     2401 ccgcgtgatg aaggcgttgt tcgccagcac cagcttgatc gcataggttg acgccgcctc
     2461 ggcagcgatg aagccggcgg attccagttc cttctcgatc gccttgccgc tggtcgcggc
     2521 cgcctcgccc gcctcttcgt agaggaggtc gttgagatac ccgtagagtt cctcttcgct
     2581 cattcccagc atctcgaagg tctatgcttt gcgtactaac atgattgtgc tgcgaggccg
     2641 tcgttggggt gctagtcggg cgtgttgtgg ctgcatgaca acccgctaag gccttggccc
     2701 ttactactgc t
//
LOCUS       DASTMD010000686         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_63319, whole genome shotgun sequence.
ACCESSION   DASTMD010000686 DASTMD010000000
VERSION     DASTMD010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63319"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VFQ54_10110"
     CDS             <1..476
                     /locus_tag="VFQ54_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165387.1"
                     /translation="WHPRAQVWQQEGPGEASVQELWGGDLAQRLAVTSALGMTGRMAE
                     RVAAGMDPEMGRAVLKLLRSARQPAMAEAGRLLSSAAERPGLALIAVDDFERASGTLV
                     QHEWAARQSGARIAHLEGVGHWWPEETPAPVAEALTNFWAELPDPERAPATDSRG"
     gene            639..>2745
                     /gene="nadE"
                     /locus_tag="VFQ54_10115"
     CDS             639..>2745
                     /gene="nadE"
                     /locus_tag="VFQ54_10115"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEU0165388.1"
                     /translation="MQNASAPMLCTAIGFRATSTEDYSRLTRRDSPRGATCRRWCASA
                     SSGIGPVSPTPLSCWIARVYPCLFGPLVVVIGDDARRGFAISAETFMRLVNIGLGNVN
                     STVGAFAANTDRCLEQAHAMAASGVTVALFPEQVIGGYPSEDFIQWQGYVEHQWTQLE
                     RYAGETASLPMVSVIGVAIAHHSLRYNCAAVVAGGRILGLVPKEKLPTYGIYYDGRTM
                     SRGTAGLHEMHNGIPFGDYVFHFDFGVLAVEVCEDLWSPDGPMKRRTYAGAELVANLS
                     ASVFRLGIDQTRRELIATRAADHQVTVAYVNAAGANDGLIFDGGGYVNQNGRLFHAAP
                     RFQQGWTSAVIDLDRTLRLRTENTTWRDDHQAFSSTNLPTTVVDVSTEIISTAESRAS
                     LRYPIPASGSFFLPPEDPAHRPTAREVLFEELLDALAMGIGDYFEKLGAFKLIGVSLS
                     GGRDSLLTLLIAHPYANRVTNGNPGELMRAFYQPSRFSSEETRLGAETICKDLGIPFE
                     IISIDDAFERELAAVEKFLDDGTGVSELTRQNIQARLRAQRMWNWSNSSGGLFLQTGN
                     MSEKAVGYTTIGGDLTGALAVLANVPKTVVMALLNYLREKHGYAGIDAVLAKPAGPEL
                     APNQAGEDELMPFPVLDACFYLFAGEKLLPDEVLSVLETMYPDRDPEQLGGYVEKFTR
                     LFLQSIYKWVQSPLALHIGN"
BASE COUNT          444 a          938 c          897 g          466 t
ORIGIN      
        1 cctggcatcc gcgggcacag gtgtggcagc aggagggtcc gggggaagcc tcggtgcagg
       61 agttgtgggg aggcgatctg gcgcagcgac tcgcggtgac gtcggccctc gggatgaccg
      121 ggcgcatggc ggagcgggtg gcggcaggca tggatccgga gatgggtcgc gccgtcctca
      181 agctgttgcg gtcggcacga cagccggcga tggccgaagc ggggcggttg ctgtcgagcg
      241 ccgctgaacg tccggggctc gcgctcatcg ccgttgacga cttcgagcga gcgagcggga
      301 cgctggtgca gcacgagtgg gcagcgcggc aatcgggcgc gaggatcgcg catctcgagg
      361 gcgtgggcca ctggtggccc gaggagacgc cggcgccggt ggctgaggct ctgacgaact
      421 tctgggcaga gttgccggat cccgagaggg ctcccgcgac cgactcccgg gggtaacgtc
      481 tgcgtaccca cggtatggca acacctggct cccggtactc gtgcggtcca accggcggat
      541 ccagatcgcg ctcgcgctca gcggcgcaga tcaacgaccg cgccgctgat agcgctcgct
      601 tgcctcgcgc cgattccggc tgggctctcg aggtcgaggt gcagaacgcg tccgcgccta
      661 tgctctgcac cgccattggc tttcgcgcga ccagcaccga ggactactcc cgcctcacgc
      721 gccgcgattc cccccgaggc gcgacctgcc ggcgttggtg cgccagcgca tccagcggaa
      781 tcggtcccgt ctcaccgacg ccgctttcgt gctggatcgc gcgcgtgtat ccttgcctct
      841 tcgggccact cgtcgtcgtc atcggtgacg acgccaggcg tggattcgca atctcagcgg
      901 agaccttcat gcggctggtc aacatcggac tcgggaacgt caattcgacc gtcggcgcct
      961 tcgccgccaa caccgatcgc tgcctggaac aggcccacgc gatggcggca tccggcgtca
     1021 ccgtggcgct cttcccggag caggtcatcg ggggataccc ctccgaggac ttcattcagt
     1081 ggcaaggcta cgtcgagcac cagtggacgc agctcgaacg gtacgccggg gagaccgcat
     1141 ccttgccgat ggtctccgtg atcggcgtcg cgatcgccca ccacagcctg cgttacaact
     1201 gcgcggcggt cgtcgccggc ggcaggatcc tcgggctcgt gccgaaggag aagctgccga
     1261 cctacggcat ctactacgac ggtcgcacga tgtcccgcgg caccgccggt ctccacgaga
     1321 tgcacaacgg catcccgttc ggtgactacg tcttccactt cgatttcggc gtgctcgcgg
     1381 tcgaggtctg cgaagatctc tggagcccag acgggccgat gaagcggcgc acctatgccg
     1441 gggccgagct cgtcgccaat ctctcggcct ccgtcttccg cctcggcatc gaccagacgc
     1501 ggcgcgaact gatcgcgacc cgcgccgcgg atcatcaggt gactgtcgcc tacgtcaatg
     1561 ccgccggtgc caacgacggc ctgatcttcg acggtggtgg ttacgtcaac cagaacggcc
     1621 gcctcttcca cgccgctccc cgcttccagc agggttggac ctctgccgtg atcgacctcg
     1681 atcgcacact caggttgcgg accgagaaca cgacctggcg ggacgatcac caggccttct
     1741 catccacgaa cctcccgacg accgtcgtcg acgtctccac ggagatcatt tccaccgcgg
     1801 aatcgcgcgc cagcctgcgc tacccgattc cggcgagcgg cagcttcttc ctgccgcccg
     1861 aggacccggc gcatcgaccg accgcacgcg aagtgctctt cgaggaattg ctcgatgccc
     1921 tcgcgatggg aatcggtgac tatttcgaga agctcggcgc cttcaagctg atcggcgttt
     1981 cgctctccgg cggtcgcgat tccctgctga cgctgctgat cgcccatccc tacgcgaacc
     2041 gcgtcacgaa cggcaatccg ggcgaactga tgcgcgcctt ctatcagccg agtcgctttt
     2101 cctcggagga gacgcgcctc ggagcggaga cgatctgcaa ggacctcggc attcccttcg
     2161 agatcatctc gatcgacgac gccttcgaac gcgagctggc cgccgtcgag aagttcctcg
     2221 atgacggcac cggcgtctcc gagctgacgc gccagaacat ccaggcgcgc ctgcgcgccc
     2281 agcggatgtg gaactggtcg aacagcagcg gtgggctctt cctccagacc ggcaacatga
     2341 gcgagaaggc cgtcggctac acgacgatcg gtggcgatct cacgggcgcc ctcgcggtcc
     2401 tggcgaacgt cccgaagacc gtggtgatgg cgctgctgaa ctacctgcgg gagaagcatg
     2461 gctacgcggg gatcgatgcc gtgctcgcga agccggcggg tccggagctc gcccccaacc
     2521 aggcgggtga ggacgagctg atgcccttcc cggtcctcga cgcctgcttc tacctcttcg
     2581 ccggcgagaa gctgctgccg gacgaagtcc tttccgtgct ggagacgatg tacccggatc
     2641 gcgatccgga acagctcggc ggctacgtcg agaagttcac ccgcctcttc ctgcagtcga
     2701 tctacaagtg ggtgcaatcg ccgttggcgc tgcacatcgg caatc
//
LOCUS       DASTMD010000687         7273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_63321, whole genome shotgun sequence.
ACCESSION   DASTMD010000687 DASTMD010000000
VERSION     DASTMD010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63321"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1059)
                     /locus_tag="VFQ54_10120"
     CDS             complement(82..1059)
                     /locus_tag="VFQ54_10120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU0165389.1"
                     /translation="MGSMKFGVFVPQGWKMDLTGISDPVEQFEAMTNVAKAADGGSWD
                     SIWVYDHFHTVPTPTQNTTFESWTISATLARDTTRVNVGQMVNCNGYRNPSLFAKIAS
                     TVDVASHGRLYAGYGAGWYEHEWRAYGYGFPETKERMAAFREGVQIIHKMWTEDTPTF
                     SGKYYTIDGPINEPKSAKPGRKVPLWIGGGGEKVTLRLVAQYGDACNVGGSPETIRQK
                     LDILQAHCEKEGRDYSEITRSTGYSAYPILPGEDAEAKTVRLRETLGNVSWEKFQQDN
                     IVGTVDDIAEKIQAGIDAGADYGIIYIPGVAYDLDPLHLFNEGVISKLG"
     gene            1418..2026
                     /locus_tag="VFQ54_10125"
     CDS             1418..2026
                     /locus_tag="VFQ54_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEU0165390.1"
                     /translation="MAGFISRGFRSRRLPPEIAARVPPGQHLTEDFPVLSAGPTPRPQ
                     LDRWDLTVDGLIDTPRRWTWEEFLALPRETFTVDIHCVTKWSKLDTVWEGVSLDTLLA
                     GAEENADYLIAHCDGGYTTNLPVEDILGGKAWITFGYDGEELEPEHGGPARLLVPHLY
                     FWKSAKWIRRLELTDRDQPGFWEELGYHNYGDPWQEQRYWGD"
     gene            2014..2742
                     /locus_tag="VFQ54_10130"
     CDS             2014..2742
                     /locus_tag="VFQ54_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEU0165391.1"
                     /translation="MGRLNWTLSEIREVIVETRRTKSLRLHVPGWENHLPGQHVDIRL
                     TAEDGYQAERSYSIASAPNGEEIEITVEELEDGEVSPYLAEELHPGDSLELRGPIGGY
                     FTWDVPDGGPLVLLAGGSGIVPLMAMLRHRANLKSDLPARLLYSARTIDDVIYRDELA
                     KLAGDAGVTVSYTLTRAQPEGWDGYTRRVDEAMLREWAWPASDRPLAYICGPTRFVEA
                     SANALIALGYDAARVKTERFGPTG"
     gene            2979..4784
                     /locus_tag="VFQ54_10135"
     CDS             2979..4784
                     /locus_tag="VFQ54_10135"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEU0165392.1"
                     /translation="MVHKYSRLVSRRSFLGGGVAALGATAAVAIPFGAQGHFYPGTTV
                     SAIDISGKSQTAAEKTLHQHLAGFEQTAVVFKHGEKTWNASLADLGYSVDYTSTLDLA
                     FQRGRDDGLVGRYVTWFNAGNNVQQFSLEFTRDNATLDAYLDTLASDVAIAARDARLV
                     QDGNEIKITPDVAGSALDKDYVRTEIEASVQSATTGTIELSMIPVTASMTAARLTPVK
                     QDAAKLLSGPVTIVNGSSSWTVDEDTLAGALVIPNDPTTEKPSLDPAKLVDAVTDIAN
                     SLYVDPVNAIVGWDNGPYAIKLGTYGQSVDPKALASAVADAADQESRTATVPLSDVLP
                     DVRSDNLDTLGLKESLASGDSSFEGSADARATNVQVAAQHVSAALIAPGDQFSFLGSL
                     GPISVDNGYVEGKIILGSWYASDIGGGVCQVSTTVYRAALRAGFEFDEWHPHSFRVSF
                     YELDGWPPGIDATIYQPNTPDEQGLDLKFTNTTGGYLLLQMTVDNQRVTATLYGTSLG
                     LDVEIPDPTVSDPIPPPAPITDPTTTLAIGKKQMLQASTPGYDVVLTRTMSKDGQVVA
                     TDTFDSNYQPQAEIWEIGATAEQVAAAGGEPGSGN"
     gene            complement(4978..5874)
                     /locus_tag="VFQ54_10140"
     CDS             complement(4978..5874)
                     /locus_tag="VFQ54_10140"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0165393.1"
                     /translation="MNRGIYAIPPTPFTESGAIDQASLAKCIDFCVTAGSAGVVTPVN
                     ASEAIALTDNERLQVAEVAVGHTAGRIPVVIGVSGVSTASSVLYTRHAREVGADAVMA
                     MPPYVRHPPADEIYAFYQEVAEAADGLPVWIQDYVGPVGTPMSPALLVRMMKEIPGVN
                     YLKEETALAPQVMSEVSTLAGDHLKGMMGGMAGRYLLEEYRRGAIGSMPACEVADVHV
                     QVWNALDAGDGEKARDLHTQLLPLLNYEAMYSFTIYKEVLKRRGVIASAATRVPGAGK
                     LDAENHRELDAILERLAPLFTV"
     gene            complement(5871..7106)
                     /locus_tag="VFQ54_10145"
     CDS             complement(5871..7106)
                     /locus_tag="VFQ54_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0165394.1"
                     /translation="MAIERIAAVEAFILVGNKDYATDAGLKTPAGVNPAARPVRALAE
                     IGDLHICVYPPQAQTCLVKITTERGTTGWGEAHAPLGPRATKAVVEDVLAPLLIGQSP
                     LAIDLHWEKMFGSMRLRGHNAGYQLEAISGVDIALWDLAGKLLDLPVYALLSGPFRTS
                     LPSYASGIPGATVEDRVRSAERFIDDGYTAMKASIGRGDIDADLAGVAAIAEVVRGKA
                     DLLVDAHGAYSLESALFVGRKLQEMGAYWLEDPLPPEDLDGYAHLVSRLDIPIAVGET
                     DCTRWQVARRLERRSASLLLPDICRAGGISEGRRIATIASTHNVKWAAHVSMGTSIHV
                     AAAAHLAAASSNFLVFEFPGSPNPLGDGLLTAPLHPENGYLAVPDGPGLGITFDDAKL
                     SEHIIDDAAYVALSAGGTR"
BASE COUNT         1275 a         2395 c         2316 g         1287 t
ORIGIN      
        1 atcccggtcc caggacacgg agaaggggcg ccggaatgat tccggcgccc cttctccggg
       61 tgttctgatc gaaacctggg gttatccgag cttcgagatc acgccctcgt tgaagagatg
      121 cagcgggtcg agatcgtagg caacacccgg gatgtagatg atcccgtagt ccgcaccggc
      181 gtcgatcccg gcctggatct tctcggcgat atcgtcgacc gtgccgacga tgttgtcctg
      241 ctggaacttc tcccaggaga cattgccgag cgtctcgcgg aggcgaaccg tcttcgcctc
      301 ggcatcctca cccggcagga tcgggtaggc gctgtagccg gtcgagcggg tgatctcgct
      361 gtaatcacga ccttccttct cgcagtgcgc ctgcaggatg tcgagcttct ggcggatcgt
      421 ctccgggctg ccgccgacat tgcaggcatc gccgtactgc gcgacgagtc gcagcgtgac
      481 cttctcaccg ccaccaccga tccacagcgg caccttgcga cccggcttcg cactcttcgg
      541 ctcgttgatc ggaccgtcga tcgtgtagta cttgccgctg aacgtcgggg tgtcctcggt
      601 ccacatcttg tggatgatct gcacgccctc gcggaacgcc gccatccgtt ccttcgtctc
      661 cgggaagccg tacccgtagg cacgccactc gtgctcgtac catcccgcgc cgtacccggc
      721 gtagaggcga ccgtggctcg caacatcgac cgtcgaggcg atcttcgcga agagcgacgg
      781 gttgcgatag ccgttgcagt tgaccatctg gccgacattc acgcgggtcg tatcacgcgc
      841 cagcgtcgcg gaaatggtcc acgactcgaa ggtcgtgttc tgggtcgggg tcggaacggt
      901 gtggaagtga tcgtagaccc agatcgaatc ccacgatccg ccgtcggcag ccttggcgac
      961 attcgtcatc gcttcgaact gctcgaccgg atcgctgatc ccggtgagat ccatcttcca
     1021 gccctgggga acaaagacgc cgaacttcat ggaacccatg gtgtctcctc atgtgcctgc
     1081 ggcggtttcc cgtctggccg gtgcgtacga aacgaacgtc cctgatcttc tgtcagccgt
     1141 gagcgcgccc gtgcaagccc ggctttccgg aagccgaata ccgatggtca tgatcgcatg
     1201 aaatcgattt catggcatcc gaagccggcc attcccactg gatcgtcgaa tccagatatg
     1261 acctgcggag gttggtgaaa ccggacgtcc tccatacaat ggaacgatgt cgcggatgcg
     1321 gcagaccggt ccgcatgatc ccaccgtcca cgacccggac cttgcggctg acatccttcg
     1381 tatgccgagc atcacgcgat cggtgaaggg aacagatatg gctggattca tttctcgcgg
     1441 ttttcgcagt cgtcgcttgc caccggagat cgccgcccgc gttccccccg gtcagcatct
     1501 caccgaggac ttcccggtgc tctcggccgg gcccacgcca cgtccccagc tcgatcgctg
     1561 ggatctcacg gtcgacggcc tgatcgacac gccgcgtcgc tggacgtggg aggaattcct
     1621 cgccctgccc cgggagacgt tcaccgtcga tatccactgc gtcacgaagt ggtcgaagct
     1681 cgataccgtc tgggaaggtg tctcactcga tacgctcctc gctggcgccg aggagaatgc
     1741 cgactacctg atcgcccact gcgacggcgg ctacacgacg aatctcccgg tcgaagatat
     1801 cctcggtggc aaggcgtgga tcacgttcgg ctacgacggc gaggaactcg aaccggagca
     1861 cggcggtccc gcccgcctgc tcgtgccgca tctctacttc tggaagagcg cgaagtggat
     1921 tcgtcgcctc gagctgaccg atcgcgatca gcccggcttc tgggaagaac tgggatatca
     1981 caactacggt gatccctggc aggagcagcg atattggggc gactgaactg gacactctcc
     2041 gagattcggg aggtgatcgt cgagacccgg cgtacgaaga gcctgcgtct ccacgtgccc
     2101 ggttgggaga atcatcttcc cggccagcat gtcgacatcc ggctcacggc ggaagacggc
     2161 taccaggccg aacggagtta ctcgatcgcc tccgcgccca atggcgagga gatcgagatc
     2221 accgtcgagg aactcgagga tggcgaggtc tcgccctacc tcgccgagga gctccatcct
     2281 ggtgacagcc tcgaactgcg cggtccgatc ggcgggtatt tcacctggga tgttcccgac
     2341 ggtggtccgc tcgtgctgct cgccggtggg tcggggatcg tgccgctgat ggcgatgctc
     2401 cggcaccgcg cgaacctgaa gagcgatctc ccggcgcgcc tcctctactc ggcccggacg
     2461 atcgatgacg tgatctaccg cgacgaactt gcgaagctcg ccggggacgc cggcgtcacg
     2521 gtttcctaca cgctgacgcg ggcccagcca gaggggtggg atggctacac ccgccgcgtc
     2581 gacgaagcga tgctgcgtga gtgggcatgg ccggcttcgg accgtccgct cgcatatatc
     2641 tgcggcccga cccggttcgt ggaggcgtcg gcgaacgcac tgatcgcgct gggctatgac
     2701 gcggcgcggg taaaaacgga acgcttcggc ccgaccgggt aacgcacgtt cccgtcaggc
     2761 accgatgcct tgacccgtcc gtcacgaaaa ggaaaccgca tgttcgcgga attcgttcac
     2821 caaagtggca agcagaggcc attgcacgct ttggttgcag ggccctcacc cagtatcgtg
     2881 tcatggagac aataccccgc cgaaaggcgt catgtgatct cgcgtgtggg gtgaccgtcg
     2941 gggatgtggt ggcggagcat aagggtcggg atacgcagat ggtgcacaag tattcacggc
     3001 tggtcagccg gcgaagtttt ctcggtgggg gagtcgccgc gctcggcgcg acggcggctg
     3061 tcgcgatccc gttcggggcg caggggcact tctatccggg aacgaccgtc tcggcgatcg
     3121 acatcagtgg aaagtcgcag accgcggccg agaagacgct gcatcagcat ctcgccggtt
     3181 tcgagcagac tgccgttgtc ttcaagcatg gcgagaaaac atggaacgcc agcctcgccg
     3241 acctcggcta ttcagtcgac tacacatcca ccctcgatct cgcctttcag cgcggtcgcg
     3301 acgatggcct ggtcggccgc tacgtgacct ggttcaacgc cgggaacaac gtccagcagt
     3361 tctccctcga gttcacgcgt gacaacgcca cgctcgatgc ctacctcgac acgctggcgt
     3421 cggatgtcgc gatcgccgcc agggacgccc ggctcgttca ggatggcaac gagatcaaga
     3481 tcaccccgga cgtcgccggc tccgcgctcg acaaggatta cgttcgcacc gagatcgagg
     3541 cgagcgtcca gagcgcgacg acgggcacga tcgagctctc gatgatcccg gtcacggcgt
     3601 ccatgactgc cgccaggctc acgccagtga aacaggacgc cgcgaagttg ctgagcggcc
     3661 ccgtcaccat cgtcaacggc agttcgagct ggacggtaga cgaggatacg ctcgccgggg
     3721 cgctggtgat cccgaacgat ccgacgaccg agaagccatc gctcgatccc gcgaagctgg
     3781 tcgacgcggt gaccgacatc gcgaactcgc tctacgtcga tccggtcaac gcgatcgttg
     3841 gctgggataa cggtccctac gcgatcaagc tcggcacgta cgggcaatcg gtggacccga
     3901 aggcgctcgc ctcggccgtc gcggatgcgg cggaccagga gagccggacg gcaacggttc
     3961 cgctctcgga cgtcctcccc gatgtccgca gcgacaacct cgacacgctg ggtctgaagg
     4021 agtccctggc ttcaggcgac tcttccttcg agggctcggc ggatgcgcgt gcgaccaacg
     4081 tgcaggtcgc ggcgcagcat gtctcggcgg cgttgatcgc gcctggcgac cagttctcct
     4141 tcctcggttc gctcggcccg atctcggtcg acaacggcta cgtcgagggc aagatcatcc
     4201 tcggatcgtg gtatgcgagc gacatcggcg gcggcgtctg ccaggtctcg acgacggtct
     4261 accgtgcggc gctccgggcc ggcttcgaat tcgacgagtg gcacccgcac tcgttccgcg
     4321 tttccttcta tgaactcgac gggtggccgc cgggaatcga cgccacgatc taccagccga
     4381 acaccccgga cgagcagggg ctcgacctca agttcacgaa tacgaccggc ggctatctcc
     4441 tgctgcagat gacggtcgac aaccagcgtg tgacggcgac actctatggc acgtcgcttg
     4501 gtctggatgt cgagattccg gacccgacgg tcagcgaccc gatcccgccc ccggcgccga
     4561 ttaccgaccc gacgacaacg ctggcgatcg gcaagaagca gatgctccag gcctcgactc
     4621 ccggctacga cgtggtactg acgcgcacga tgtcgaagga tggccaggtc gtcgcgaccg
     4681 acaccttcga cagcaactac cagccgcagg cggaaatctg ggagatcggg gcgacggcgg
     4741 aacaggttgc cgccgcgggt ggcgaacccg gctccgggaa ctgatccact tcaggacggc
     4801 gccacggcgc gcatccggcc gtttccgtgg cgggtcaccc cgtgtgaccc atcgggcgca
     4861 gcccgatatg accgtcgcat gggcggtgtc cccgccgccc gtcttcccgt cccggcggcg
     4921 acccacgtgg tcgccgccgg gacgggacca ggcgacagga tgtttcgggt cgatgcgcta
     4981 caccgtgaag agcggggcga ggcgttcgag aatcgcgtcc agctcgcggt gattctcggc
     5041 atcgagcttg ccggcgccgg gaacacgcgt cgccgccgag gcgatcacac cgcgtcgctt
     5101 caggacttcc ttgtagatcg taaacgagta catcgcctcg taattgagga gcggcagaag
     5161 ctgggtgtgg agatcccgcg ccttctcgcc atccccggca tccagcgcgt tccagacctg
     5221 aacgtgaacg tccgcgacct cgcaggcagg catgctgccg atcgcgccac gccggtattc
     5281 ctccaggagg tagcgaccgg ccatgccgcc catcatcccc ttcagatggt ctcccgccag
     5341 tgtggagacc tccgacatca cctgcggggc gagcgcagtc tcctccttga ggtagttgac
     5401 gccggggatc tccttcatca tccgcaccag cagcgccggc gacatcggcg tgccgaccgg
     5461 cccgacgtaa tcctggatcc agaccggcaa gccgtccgcc gcttcggcga cttcctgata
     5521 gaaggcgtag atctcgtcgg ccggtggatg cctcacatat ggcggcatcg ccatcacggc
     5581 atcggcgccg acctccctcg cgtggcgggt gtagagaacg gatgaggccg tgctgacccc
     5641 ggaaacgccg atcacgaccg ggatccgccc ggcagtgtga ccgaccgcga cctccgcgac
     5701 ctgcagccgc tcgttgtcgg tcagggcgat cgcctcgctt gcgttcacgg gtgtgacgac
     5761 ccccgccgat ccggcggtga cgcagaaatc gatgcatttc gccagcgatg cctggtcgat
     5821 cgcgccgctc tccgtgaatg gcgtcggtgg aatcgcgtag ataccccggt tcatctcgtt
     5881 cctcctgcgc tcagcgcgac atacgccgca tcatcgatga tgtgttcgct cagtttcgcg
     5941 tcgtcgaagg tgatccccag tcccggaccg tccggcaccg cgagatatcc gttctccggg
     6001 tgcagcggcg ccgtcagcag gccgtccccg agcggattcg gcgatcccgg aaattcgaag
     6061 acgaggaaat tggatgatgc cgccgccaga tgcgccgccg ccgcgacgtg gatcgaggtg
     6121 cccatgctga catgggcggc ccacttcacg ttgtgggtgc tggcgatggt tgcgatccgg
     6181 cgtccttcgg agatgccacc ggcgcggcag atatccggca gcaggagact cgccgagcgt
     6241 cgctcgagtc gccgcgcgac ctgccagcgg gtgcaatcgg tctcgcccac ggcgatcggg
     6301 atatcgagcc gggagacgag atgcgcgtag ccatcgaggt cctccggcgg cagcggatcc
     6361 tccagccagt aggcgcccat ttcctgcagc ttccgtccga caaagagcgc gctttccagg
     6421 gaataggcgc cgtgggcgtc gacgaggaga tcggctttcc cgcgcacgac ctcagcgatc
     6481 gccgcgaccc cggcaagatc ggcgtcgatg tcgccacggc cgatcgacgc cttcatcgcc
     6541 gtgtagccat catcgatgaa gcgctcggcg ctgcggacac ggtcctcgac ggtcgcgccc
     6601 gggatgccgg acgcatagga cggcaatgac gttcggaagg gaccgctcag gagcgcgtag
     6661 acggggagat cgagcagctt gccggccaga tcccagagcg cgatatcgac gccggagatc
     6721 gcctcgagct ggtagccggc attgtgtccg cgcagccgca tgctgccgaa catcttctcc
     6781 cagtgaagat cgatcgcgag cggggattgt ccgatcagga gaggggcgag gacatcctcg
     6841 acaaccgcct tcgtcgcgcg cggacccagc ggcgcgtgcg cctcgcccca cccggtggtg
     6901 ccacgctcgg tcgtgatctt cacgaggcag gtctgcgcct ggggcggata gacgcagatg
     6961 tgaagatctc cgatctccgc caacgcgcga accggtcttg ccgccgggtt gacgcctgcc
     7021 ggtgtcttca acccggcatc ggtcgcgtaa tccttgtttc cgacgaggat gaacgcctcg
     7081 acggccgcga tccgctcgat cgccattgca cgcccctttc cgttggtccg gtctgtctca
     7141 gtctggtctc acgatacccg aggacgggat acctgtgaat tccgagagcg accggagact
     7201 ggcgcgcgac atgcggtctc cacggaatcc gaacgattcg tggaaaggca cggcgatgac
     7261 cggtcaggca ttc
//
LOCUS       DASTMD010000688         4879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1135378, whole genome shotgun sequence.
ACCESSION   DASTMD010000688 DASTMD010000000
VERSION     DASTMD010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135378"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..227)
                     /locus_tag="VFQ54_10150"
     CDS             complement(12..227)
                     /locus_tag="VFQ54_10150"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU0165395.1"
                     /translation="MFNIGLYRSRKHKVIFGVCGGIADQFGVSPFWVRIATVIGAIVI
                     PGISIWPIVALYIALGLALPVREDTYI"
     gene            complement(270..1259)
                     /locus_tag="VFQ54_10155"
     CDS             complement(270..1259)
                     /locus_tag="VFQ54_10155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0165396.1"
                     /translation="METTHPARNIPPSSPFIRTAQPGDLVDAAQVYQAAAKALHESLH
                     ADDPWSSEDARRIDLQAAIEALQAINRERPEAVQIAEVDGEIVGIGAAVIRERHAHIL
                     FLFVDPMRQRAGIGRALLEKLQTVIVASGCDLITLTASEDRKAWRRYMAMGLQPGAPI
                     ISMRAPAAVFPELPWQDGLEPHAISLERPDLLGTIGDIDRVVKGVRRMADFERWFRDE
                     HASGELLTRRSDGIPVGYYLISRGAKFGSIGPVAAMDESRFGDVLTRALHAAGTIDGK
                     RELVWRADVPSMNRAAIEPLFAAGFYPWNLLRWFANGEIGRWDRYIFRDEDQL"
     gene            complement(1447..2217)
                     /locus_tag="VFQ54_10160"
     CDS             complement(1447..2217)
                     /locus_tag="VFQ54_10160"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HEU0165397.1"
                     /translation="MTQPSKPQPTSPDADTLDSEPSRINRRRTLGAITASAAAATAVA
                     VGVLARPGASAQSDETPTPTATTDANSGIVPIQSTTVGEMPTTGAIRGAPIGTQPQQL
                     VATTATLPVAIQVPKAQIDAQIEVLDIVNGAMQNPTGPWVVSWYEQTSELGVVGNVVV
                     AGHVDYWNVGPSVFYNIRNLVKGDEITLTGASNTTYTYVVDTNRIYGVQELTSGALAD
                     LVSATTTSVLTLFTCGGEFDYVNGEYLSRTVVRANLKA"
     gene            2412..3392
                     /locus_tag="VFQ54_10165"
     CDS             2412..3392
                     /locus_tag="VFQ54_10165"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU0165398.1"
                     /translation="MSETETPWYLHEIIRTALGTPGRLAGAISLPSGTVNEVWRLEIA
                     EVGDAILRIAPSDVAAASGPSWLTSFGLRREQGVIALLPDLAAILPGTIHADFSRTIL
                     DRDWAIQRLVPGRPWSEVDAELTQEQQLDLWRQSGTICRAIHGVRRGEFGGVAGPETG
                     LRSRSWAGVLLADGRGFIDDAARFGLDAAPFIALRDAIARHRETLDLVTTASLIHSDL
                     GPRHLFVEPDDRGDWKIVGLIDLEFGRFADPMSESQIEVFDLIPPDPVFQEAFWQGYG
                     AVDHPPGREIRSLVYQAIVLGWVVTDFARLDRPGEIPSVLVELATRLADL"
     gene            complement(3440..4132)
                     /locus_tag="VFQ54_10170"
     CDS             complement(3440..4132)
                     /locus_tag="VFQ54_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165399.1"
                     /translation="MAGGTMAGTIRVMLEIGPKGKRAVAVAPDWPGLERGAKTEAEAV
                     DRLLAYVPRYANIARLAGMGDEFGDATSGEVVETYPGVGSTDFWGISFAFSQIDRQEI
                     SSDELERQLTLMQACWSFFDDVRSRVSPEMKKGVRGGGRDRDHIVRHTVGEELGWAEK
                     LGIRWPPGVITIDDGLQKHRDSYCAAIRSYHAEGKMARKWPLQYLIRHTAYHTMDHAW
                     EMEDKDLSADLA"
     gene            complement(4230..>4879)
                     /locus_tag="VFQ54_10175"
     CDS             complement(4230..>4879)
                     /locus_tag="VFQ54_10175"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0165400.1"
                     /translation="GSITFIAACGAWFFVTQMAIGKRVRGPFPLSTFVILIVTSAITL
                     LQFIDHDVLTSLERHTGDLSSGRYWKLFTPLFVQSDGWGDAITNTIAYLIVAFVAERV
                     YGRRRVLLMYFIPGIVGDAFGFAWTDHGAANSVAIAGLIGGFLMLELVHEHRLPRIAP
                     ILAIVGLLGAVTLCLLSEIHGPAILTGAVLGYALNHVSPMLPHHAAPPVTAAATT"
BASE COUNT          831 a         1557 c         1598 g          893 t
ORIGIN      
        1 gcgcgaaggg actagatgta ggtgtcttcg cgcaccggga gggccaggcc gagtgcgatg
       61 tagagcgcga cgatcggcca gatgctgatg cccgggatga cgatcgcgcc gatgacggtg
      121 gcgatccgca cccagaacgg gctgaccccg aactggtcgg cgatgccgcc gcagacgccg
      181 aagatcacct tgtgcttgcg agaccggtac aaaccgatgt tgaacatgaa tgaaaccctt
      241 tccgaacgcg tgaatgcgcc ctcaatagtt cagagttgat cttcatcacg gaagatatag
      301 cgatcccacc ggccgatttc gccgttcgcg aaccatcgga ggaggttcca cggatagaac
      361 ccggcggcga agagcggttc gatggccgcc cggttcatcg aaggaacgtc cgctcgccag
      421 acgagttccc gcttcccgtc gatcgttccc gccgcgtgca gtgctcgcgt cagaacgtcg
      481 ccgaaccgcg actcgtccat cgccgcgacc gggccgatac tcccgaactt cgcgccgcgg
      541 gagatgaggt aatagccaac cgggatgcca tcgctgcgcc gggtcagcag ttcaccggag
      601 gcgtgctcat cgcggaacca gcgctcgaaa tcggccatcc ggcggacgcc tttcacgacc
      661 cggtcgatgt cgccgatcgt cccgagcaga tcggggcgtt cgagcgagat cgcgtgcggc
      721 tcgaggccgt cctgccaggg aagctccggg aagaccgccg ccggcgcccg catcgagatg
      781 atcggtgcgc cgggttggag acccatcgcc atgtagcgtc gccacgcctt gcgatcctcg
      841 gaggcggtca gcgtgatgag atcgcagccg gaagcgacga tcacggtctg gagcttctcc
      901 aggagggctc gtccgatacc ggcccgctgc cgcatcgggt cgacgaagag gaagaggata
      961 tgcgcgtgtc gctcgcggat gaccgccgcg ccgatgccga cgatctcgcc atcgacctcg
     1021 gcgatctgaa ccgcttccgg acgctcgcga ttgatcgcct ggagtgcctc gatcgccgcc
     1081 tggagatcga tccggcgtgc atcctcggaa ctccagggat catcggcatg aagcgattcg
     1141 tgaagcgcct tcgccgcagc ctggtagacc tgggcggcgt cgaccagatc ccccggctgc
     1201 gccgtgcgga tgaaaggaga cgacggggga atgttgcggg ctgggtgcgt cgtttccatg
     1261 acgtcagtat cgctccgggc tgtgtgggat tcctggcgga catcccccaa ttgcgcgtgc
     1321 ctgtttgccg acaagcgtac taccgtcgac cttcccgttc gactttggtg tccgacgtca
     1381 atgccccgtg tcgacatcga cacggggctt ctggccgagc tggctggtct ggaggcggag
     1441 tagcggctag gccttgagat tggcccggac gaccgtgcgt gagagatatt cgccgttcac
     1501 gtaatcgaac tcgccgccgc aggtgaagag ggtcagcacg ctcgtcgtcg tcgcgctgac
     1561 gagatcggcg agagcgccgc tcgtcagttc ctgcacgccg tagatcctgt tcgtgtcgac
     1621 gacataggta taggtcgtgt tgctggcgcc ggtgagcgtg atctcgtcgc ccttcaccag
     1681 gttccggatg ttgtagaaaa cggacggacc gacgttccag tagtcgacgt gacccgcaac
     1741 cacgacgttg ccgacgaccc cgagctcgga cgtctgctcg taccaggaga cgacccatgg
     1801 tccggttgga ttctgcatcg caccgttgac gatatcgagc acctcgatct gggcgtcgat
     1861 ctgggctttc gggacctgga tcgcaacggg cagcgtcgcc gtcgtcgcga cgagctgctg
     1921 cggctgcgtc ccgatgggag cgccgcgaat ggcgccggtg gttggcatct caccaaccgt
     1981 cgtcgactgg atcggtacga taccggaatt cgcgtcagtc gtcgcggtgg gcgtcggtgt
     2041 ctcgtcgctc tgtgcgcttg cacccggccg cgcgaggact ccaaccgcga ccgcggtggc
     2101 ggccgcggca ctggccgtga tcgcgcccag cgtccggcgc cggttgattc ggctcggttc
     2161 cgagtcaagg gtgtcggcgt cgggcgaggt tggctgaggc ttgctgggct gggtcatgaa
     2221 gcgctcctgg tcgaagggct ggccgcatcg tgaggtgcga tgcactcgcc gtctcacgcc
     2281 tgtcactata aggacgcatg aaccgcgcgt ttcgttttgt ctccggtcca gcataggaag
     2341 cggatacgtt cctgtcgagt gcgttcacat gatgatgagg aagctggttc tccgggaggg
     2401 gactccgaga aatgagtgag accgagacgc cctggtacct ccatgagatc atccggaccg
     2461 cacttggtac cccgggccgg ctggcgggag cgatttccct gcccagcggc acggtgaacg
     2521 aggtctggcg gctggagatc gccgaagtcg gcgacgccat cctccggatc gcgccatccg
     2581 atgtggctgc cgcgagcggt ccgagctggc tgacctcgtt cggtctccgg cgtgagcaag
     2641 gcgtgatcgc cctgcttccc gacctcgcgg cgatcctccc ggggacgatt cacgccgact
     2701 tttcgcgcac gatcctcgat cgcgactggg cgatccagcg gctcgttcct ggacgtccct
     2761 ggagcgaggt cgacgcggaa ctcacccagg agcagcaact cgatctctgg cgtcaatcgg
     2821 gcacgatctg ccgggcgatt cacggggtgc gacgcggcga gtttgggggt gtcgcggggc
     2881 cggagaccgg cctccggagt cgatcctggg ccggcgtgct gcttgccgac ggtcgcggtt
     2941 tcatcgacga tgccgcgcgt ttcggtctcg atgccgcgcc ttttatcgcg ttgcgggatg
     3001 ccatcgcgcg tcatcgcgag acgctcgatc tcgtgacgac ggcatcgctg attcacagcg
     3061 atctcggccc gcggcatctc ttcgtcgagc cggatgatcg cggcgactgg aagatcgtcg
     3121 gcctgatcga tctcgagttc ggacggttcg ccgacccgat gtcggaaagc cagatcgagg
     3181 tgttcgacct catcccgccc gacccggtct tccaggaagc cttctggcag ggatacggtg
     3241 cggtcgacca tccgccgggc cgggagatcc gaagcctggt ctaccaggcg atcgtgctcg
     3301 gatgggtggt caccgatttc gcccgcctcg accgcccggg cgagattccg tctgtcctgg
     3361 tcgagctggc gacgcgtctc gccgatctct agtccgccac ggatcaagcc gggggagaga
     3421 tggtgcttgc ggcgtcatat cacgcgagat cggcggagag atccttgtct tccatctccc
     3481 acgcgtgatc catggtgtga taggcggtat gccgaatcag gtactggagc ggccacttcc
     3541 gggccatctt gccttcggca tggtaggacc ggatcgcggc gcaatagctg tcgcgatgct
     3601 tctgcaaccc gtcgtcgatc gtaatgacgc ccggtggcca gcgaattccg agcttctccg
     3661 cccatcccag ctcctcgcca accgtgtgcc ggacgatgtg atcgcggtct cgcccgccac
     3721 cgcgaacgcc cttcttcatc tccggggaaa cgcgcgagcg cacgtcgtcg aagaacgacc
     3781 agcacgcctg catcagtgtc agttggcgct cgagctcgtc actggagatc tcctgacggt
     3841 cgatctgcga aaaggcgaag gagatacccc agaagtcagt cgatcccacg cccggatagg
     3901 tctcgaccac ttcgccggat gtcgcgtcgc cgaactcatc ccccattccg gccagcctcg
     3961 cgatgttcgc gtagcgcgga acgtaggcaa ggaggcggtc gaccgcctcc gcttccgtct
     4021 ttgccccacg ctcgagtcct ggccaatccg gcgcgacggc cacggcgcgc ttccccttcg
     4081 gtccaatttc gagcatcacc cggatcgtgc cagccatcgt tccccctgcc atggatctct
     4141 ccaccattct cggcatcgaa cagacgtcgc cccaccgtag cattcaagca ggacacccag
     4201 tgtccgcatt ttcgcttcct ggtgatggat caggttgtgg cggcggcggt cacgggtggt
     4261 gcggcgtggt ggggcagcat cgggctgacg tgattgagcg cgtatccgag aacggccccg
     4321 gtcaggatcg ccgggccgtg gatctcgctg agcaggcaga gcgtgacggc gccaagcaat
     4381 cccacgatcg ccaggatcgg cgcgatcctt ggcagtcgat gctcgtgaac cagctccagc
     4441 atgaggaagc cgccgatcag gcccgcgatc gccaccgagt tcgccgcgcc gtgatccgtc
     4501 caggcaaagc cgaacgcatc gccgacgatt cccgggatga agtacatcag cagcacgcga
     4561 cgtcgcccgt agacgcgctc cgccacgaag gccacgatca ggtaggcgat cgtgttcgtg
     4621 atcgcgtcac cccagccgtc gctctggacg aagagcggcg tgaagagctt ccagtagcga
     4681 cccgacgaga gatcgccggt gtgccgctcg agactggtca ggacatcgtg gtcgatgaat
     4741 tgcagcaacg tgatcgccga cgtcacgatc aggatcacga aggtcgagag cgggaatggt
     4801 ccacggacgc gcttcccgat agccatttgg gtcacgaaga accacgcgcc gcacgcggca
     4861 atgaacgtga tcgatccga
//
LOCUS       DASTMD010000689         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1759917, whole genome shotgun sequence.
ACCESSION   DASTMD010000689 DASTMD010000000
VERSION     DASTMD010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759917"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VFQ54_10180"
     CDS             <1..1017
                     /locus_tag="VFQ54_10180"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HEU0165401.1"
                     /translation="EVLDGLRRTPSSALGTGRYKLKDGDLDRALDLLSAHNITAMVYI
                     GGNDSADTAHRLAERARERKQNLQVISVPKTVDNDLPETDHCPGYPSMARYLANTVRD
                     ATYDTLAAPHLYPVKFIEVMGRDAGWVAAAGALGFTETERAADLAPRILFPERPPESA
                     GVVLDAVRADLARRGWTVVVVPETLHAAGGAHLGGAEPDYVDEFGHPYYPSTGAALAR
                     LVTQHLGVRARFEKPGSAARMSISLVSEVDLDEAYRLGGAAVERILAGETARMTALRR
                     IANDPYATTIASVPVELIANRVRHLPDEFIHAGDMDITDAFREWATPLLGARPFPPYA
                     RIGQ"
     gene            1116..1472
                     /locus_tag="VFQ54_10185"
     CDS             1116..1472
                     /locus_tag="VFQ54_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165402.1"
                     /translation="MTGINDDEESSRQQSSKHPIADLSEGLRVAAKQPRPRADDPSWS
                     PRCGCDVIARCGHKVGEIVEVESGYIVVEVGFFHPSDLYVPIGAIDDHDDDDVFLTLT
                     KGEVLHAGWDVEPIQP"
     gene            2136..>2771
                     /locus_tag="VFQ54_10190"
     CDS             2136..>2771
                     /locus_tag="VFQ54_10190"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0165403.1"
                     /translation="MQGQVALVTGGGGAGIGRTICQRFAAEGATVIAADRDFPAAEET
                     ARLIAASGGTSLAMAMDVSNGEQVTDGIATAIARFGKIDILVCSAGFSDGTDILTIDE
                     DRWDRNLTINLKGPFMCAKAVLPGMIERGSGVILTISSVNGLTGMGEEPYSAAKAGLV
                     NLTRNLAVRYGKHGIRANCICPGTIRTYKWDQRLEREPTALDAMTRWYPLGR"
BASE COUNT          498 a          941 c          872 g          460 t
ORIGIN      
        1 gaggtgctgg acggcctccg gcgcacgccg tcgtccgcgc tcggaacggg tcgctacaaa
       61 ctgaaggatg gcgatctcga tcgggcactc gaccttctct ccgcgcacaa catcacggcg
      121 atggtctaca tcgggggcaa cgactcggcg gacacggcgc atcgtctcgc ggagcgggcg
      181 agggagcgga agcagaatct ccaggtcatt tccgtaccga agaccgtcga caacgacttg
      241 ccggaaacgg atcactgccc gggctaccca tccatggcgc gctatctcgc gaacacggtt
      301 cgcgacgcta cctacgacac gctcgccgcc ccgcatctct acccggtcaa gttcatcgaa
      361 gtgatgggcc gcgatgcggg ctgggttgcc gccgccggcg ccctcgggtt cacggagacg
      421 gagcgtgccg ccgacctcgc gccccggatt ctcttccccg aacgcccccc ggaatcagcc
      481 ggcgtcgtgc tggatgccgt gcgcgcggat ctggcgcgac gcggctggac cgtggtcgtg
      541 gtgccggaga cgctccacgc cgccggcggc gcccatctcg gcggcgccga acctgattat
      601 gtcgacgaat tcggacatcc ctactatccg agcaccggcg ccgctctcgc ccggctggtg
      661 acgcaacatc tcggtgtccg cgcacgcttc gagaagccgg gaagcgcggc ccggatgtcg
      721 atctcgctcg tctcggaggt cgatctcgac gaggcgtaca gactcggcgg cgccgcggtc
      781 gagcgcatcc tcgccggcga gaccgcacga atgacggcac tcaggcggat cgcgaacgat
      841 ccatacgcga cgacgatcgc gagcgtgccg gtcgaactga tcgccaaccg cgtgcgccat
      901 ctccccgatg agttcattca cgccggcgac atggacatca ccgatgcgtt tcgcgaatgg
      961 gcgacgccgc tgcttggagc gcgtccgttc ccaccctatg ctcgaattgg ccagtagatt
     1021 cacacgaatc aatgcatctg acccggtgat gtggcagaat cacgtcggca cgcgatgtgg
     1081 cgtgacgttt tgatgtacca ggcaggagcg tgccggtgac cggcatcaat gacgatgagg
     1141 aatcctctcg tcagcaatcc tcgaagcacc cgatcgcgga tctcagcgaa ggtctgcgcg
     1201 tcgcggccaa acagccccga cccagggccg acgacccgag ttggtcgcca cggtgcggat
     1261 gtgacgtcat cgccagatgc ggacacaagg tcggcgagat cgtcgaggtt gaatccggat
     1321 acatcgtggt tgaggtcggg ttcttccacc cgagcgatct ctacgtcccg ataggcgcga
     1381 tcgacgacca cgacgacgac gatgtcttcc tgacgctcac caagggcgaa gtccttcacg
     1441 ccggctggga tgtcgaacca atccagccct gaccccgact cgccattccc ctcatcccac
     1501 cgagttcccc actcccgatt cccgtcctca cggcccttgt ggccgttccc catcgacgcc
     1561 gccaagaggc gccgcgccat cggcgtgttg gaggggaccg ctgcgttcgc ggcgaaagat
     1621 aaacgtccgc ccggcaaacc cgggcattcc gacgacgatc caaacaaccc cgtcatcctg
     1681 agcttgtcga aggatccccg ggcgaagccc acccggcaca gccgggcccc aacacgacct
     1741 caccgtgccc gatgtttccc acaccgccga tcggcgacac cgatccgctg gcgaaacgcc
     1801 tcgttccgga gttggtgtct gtgtccgcct gcggcggatc ggcttcgccg gggggtcctt
     1861 ccttgcggtc aggatgacgg atccggggtt gccgcagaaa caggtgcata ccaacctgtc
     1921 tctggcgagg cccggcccga tctcctcgtg cgcattcccg atcccgtcct cacggccctt
     1981 gtggccgttc cccgtcggcg ctgccactat gcctccggag gatgccacgc gtgaaggtac
     2041 ggcgttttcg gatgatggac cggcgaccgg ctcgatgcga tagtgttccc caattggaag
     2101 tcgccgtgtc gattggggaa agaatgtcca cgaacatgca ggggcaagtg gccctggtga
     2161 ccggcggtgg cggcgccggg atcggtcgta cgatctgtca gcgattcgcc gccgaagggg
     2221 ccaccgtgat tgccgccgac cgggattttc cggcggcgga agaaaccgcg cggctgatcg
     2281 cggcgagtgg cggaacctcg ctcgccatgg cgatggacgt ctccaacggc gaacaggtga
     2341 cggacgggat cgcgacggcc atcgcgcgct tcggcaagat cgacatcctc gtctgcagcg
     2401 ccggattctc cgacggcacc gatatcctca ccatcgacga ggaccgctgg gatcggaacc
     2461 tgacgatcaa tctcaaggga cccttcatgt gcgcgaaggc ggtccttccc gggatgatcg
     2521 aacgcggatc gggcgtcatc ctcacgatca gctcggtcaa cgggctcacg gggatgggtg
     2581 aggagccata cagcgccgcg aaggcgggac tcgtgaatct cacgcggaac ctcgccgtcc
     2641 gctacggcaa gcacggcatc cgcgcgaact gcatctgccc gggcacgatt cgcacctaca
     2701 agtgggatca gcgcctggaa cgtgaaccaa ctgcactcga cgccatgacg cgctggtacc
     2761 cgctgggacg t
//
LOCUS       DASTMD010000690         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1135438, whole genome shotgun sequence.
ACCESSION   DASTMD010000690 DASTMD010000000
VERSION     DASTMD010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135438"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFQ54_10195"
     CDS             complement(<1..534)
                     /locus_tag="VFQ54_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU0165404.1"
                     /translation="MLFIVLAILAGTIGTLPGDEWMTHQVQRLHGALVHDYVRVVTVF
                     GTTRLALPLLVVALVVTVVGRHWREMSFLLILFLLRLLCSTVKPIVASPRPDLTQAII
                     RDFPGGFGFPSGHTLTSAVLFGGAAFMAMRFLPWRSRALPWIVGVAWVAGAASTGFAR
                     IWEGAHWTSDVVGGALLG"
     gene            complement(597..1292)
                     /gene="rpiA"
                     /locus_tag="VFQ54_10200"
     CDS             complement(597..1292)
                     /gene="rpiA"
                     /locus_tag="VFQ54_10200"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU0165405.1"
                     /translation="MDSAERLRRVGERAAAEIVDGMIVGLGTASTAEAMVRAVGIRVA
                     DGLKMTGIATSERTAELARSLDIPLVALDDVDRIDLCIDGADEINPDVDVVKGRGGAL
                     LYEKLVARQADRYIIIASHEKLVAKLGVRLPLPVEVVPVGWKYTGESIAKLGVTQKLR
                     LTAAGDAYVTDGGHYIFDCDAPAGGFADPGGLATALKAIVGVVEHGLFIGMADLALTI
                     DEDGIVTETSRPR"
     gene            complement(1285..2094)
                     /gene="pgl"
                     /locus_tag="VFQ54_10205"
     CDS             complement(1285..2094)
                     /gene="pgl"
                     /locus_tag="VFQ54_10205"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU0165406.1"
                     /translation="MGQTIKDAGRRIDYGERGTVVVVANGDVLADVAAETLISTIAEA
                     AGEERDAIVALSGGSTPKKMGSLLAAPSYAGRVHWDHVQFFWGDERWVPIADAESNAG
                     EAFRGFLEKVPVPAENVHPFETDEGTDPEGVADRYERLIREITDTMNPEETPVFDLIF
                     LGMGDDGHTLSLFPGTVAMHEQDQLVLAHYVPKLDTTRLTFSPRLANATKTAVFLVGS
                     SGKADMLHTVLDGPIDIDTTPSQVIRPTDGQLIWLVDVAAAARLDRTPANG"
     gene            complement(2344..>2769)
                     /locus_tag="VFQ54_10210"
     CDS             complement(2344..>2769)
                     /locus_tag="VFQ54_10210"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpcA/G6PD domain-containing protein"
                     /protein_id="HEU0165407.1"
                     /translation="GLSAALLAVGWLASRLGWDAIEPLDTNKAGVSWAPLRARGTSGR
                     QRDIQIRLTPDNSPQARFSLRRVELIATGEAPGVFRVERTDDDDLVTSSETPSVPFVS
                     RMVYSKRPENARMLHSEVQRLSSDRVYEEALRTAAKLLP"
BASE COUNT          491 a          977 c          870 g          431 t
ORIGIN      
        1 gccgagcagc gcaccgccga ccacgtcgct cgtccagtgc gcgccctccc agatccgcgc
       61 gaagccggta ctcgccgcac cagctaccca cgcgacgcca acgatccagg gcagagcgcg
      121 cgatcgccac ggcaggaagc gcatggccat gaacgccgct ccaccgaaga gcacggcact
      181 cgtcaacgtg tggccactcg ggaagccaaa gccacccggg aagtcccgga tgatcgcctg
      241 ggtgagatcg ggtctcgggc tcgccacgat cggcttcacg gtcgagcaga gcagacgcag
      301 caggaagaga atcagcagga atgacatctc ccgccagtga cgtccgacga cggtgacgac
      361 cagtgcgacg accagaagcg gcagcgcgag gcgcgtcgtt ccgaagacgg tgacaacccg
      421 aacgtagtcg tggacgagcg cgccatgcag gcgctggacc tgatgcgtca tccactcgtc
      481 acccgggagc gtgccgatcg tgccggccag gatcgcgaga acaatgaaaa gcaccgcgag
      541 cactccaatg agcgcccacg gtgcagtcga gattcgatcg ggaccagcgt ccgatctcat
      601 cggggtcggc tggtctccgt cacgatgccg tcttcgtcga tcgtcagcgc gagatcggcc
      661 atgccgatga acaggccgtg ctcgacgacg ccgacgatcg ccttcagcgc cgtcgccaga
      721 cctcccggat cggcaaagcc acccgccggc gcgtcgcaat cgaagatgta gtgaccgccg
      781 tccgtcacat aggcgtcacc ggccgcggtc aggcgcagct tctgcgtcac accgagcttc
      841 gcgatcgact ccccggtgta tttccagcca accggaacga cctctacggg cagcggcagc
      901 cggacgccga gcttcgcgac cagcttctcg tgcgaggcga tgatgatgta ccggtccgcc
      961 tgccgcgcga ccagcttctc gtacagcagg gcgccgccgc gccccttcac gacgtcgacg
     1021 tcgggattga tctcgtcagc gccatcgatg cagagatcga tccggtcgac gtcatcgagc
     1081 gcgaccagcg ggatgtcgag actccgggcg agctcggccg tgcgctccga ggtcgcgatc
     1141 ccggtcatct tcaggccgtc agcgacgcgg atgccgactg cccgcaccat cgcctccgcc
     1201 gtcgaggcgg tgccgagacc gacgatcatg ccgtcgacga tctcggccgc ggcgcgttcg
     1261 ccgacgcggc gcagacgctc ggcgctatcc attggcaggg gtccggtcga gcctggccgc
     1321 cgccgccaca tcgacgagcc agatcagctg accgtcggtc ggtcggatca cctgcgaggg
     1381 cgtcgtgtcg atgtcgatcg gtccgtcgag gacagtgtgc agcatatccg ccttgccgga
     1441 gctaccaacc aggaagaccg ccgtcttcgt cgcattcgcg aggcgcggtg agaacgtcag
     1501 ccgcgtggtg tcgagcttgg ggacgtagtg ggcgagcacc aactggtcct gctcgtgcat
     1561 cgcgaccgtg cccgggaaga gcgagagggt atggccatca tcacccatcc cgaggaagat
     1621 caggtcgaag accggcgtct cttccggatt catcgtgtcc gtgatctcgc ggatgagccg
     1681 ctcgtagcga tcggcgacac cctcgggatc ggttccttcg tcggtctcga agggatggac
     1741 attctcggcg ggaacgggga ccttctcgag gaagccgcgg aacgcctcgc cggcgttgct
     1801 ctccgcatcg gcgatcggca cccaacgctc gtcgccccag aagaactgga cgtgatccca
     1861 atgcacgcgc cccgcatacg agggtgcggc gagcagcgat cccatcttct tcggcgtcga
     1921 accgccggag agcgcgacga tcgcgtcccg ctcttcgccc gcggcctcgg cgatcgtcga
     1981 gatcaacgtc tcggcggcca catccgcgag cacatccccg ttagccacga cgacgaccgt
     2041 tccacgctcg ccgtagtcga tgcgtcgacc ggcgtccttg attgtctgtc ccatgtctcg
     2101 ttctccatca gttgaaacca gcgacgaatc ggtaggggta ggggccagcc tggtgctgac
     2161 ccgcggccgc aggccgcctc ggcgggttcc gtccgatccg cgatccgacg gcgcgttcca
     2221 ccgacattgg gccgggcgac cacccctgcg ctgctgccgc acagacggtc aggtcatcta
     2281 cggaatgtac cgccggacca cataacgggg aaggggacgg tcgatgaccg cccccccttt
     2341 gcctcacggc agcaacttcg ccgccgttcg cagtgcttcc tcgtaaacgc gatcgctcga
     2401 aaggcgctgg acctccgaat gcagcatccg cgcgttctcc ggccgcttcg agtacaccat
     2461 gcggctcacg aacggcaccg aaggtgtctc gctgctcgtc acgaggtcgt catcgtccgt
     2521 gcgctcgacg cgaaagaccc caggcgcttc acccgtcgcg atcaactcga cccggcggag
     2581 cgagaagcgc gcctgcggac tgttatccgg cgtcaggcga atctggatat cccgctgcct
     2641 gccactggtt ccccgcgccc gcaacggcgc ccaggaaact cccgccttgt tcgtgtccag
     2701 cggctcgatt gcgtcccaac caaggcgact cgcgagccat cccaccgcca gcaatgccgc
     2761 cgacagccc
//
LOCUS       DASTMD010000691         4031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2831682, whole genome shotgun sequence.
ACCESSION   DASTMD010000691 DASTMD010000000
VERSION     DASTMD010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2831682"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..588)
                     /locus_tag="VFQ54_10215"
     CDS             complement(82..588)
                     /locus_tag="VFQ54_10215"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEU0165408.1"
                     /translation="MGVSGSGKSTIGALLAKKLDAGFTDGDRFHSASNIAKMAAGAPL
                     DDSERLPWLEAIGKHLAASASSGSTEIVACSALKRQYRDVLRSYSPGLFVVFLDGPSD
                     VIRERIARRNHAFMPSSLLESQLASLEPLGPDELGIRVDIMLPPEQIVGEVIARLSVE
                     SGALSTTR"
     gene            797..1585
                     /locus_tag="VFQ54_10220"
     CDS             797..1585
                     /locus_tag="VFQ54_10220"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEU0165409.1"
                     /translation="MNEPLNEDAGPDDGSDEFAPSGRGLHAKVLDQLGLAVCGQEFPT
                     NTVMTIEDIERRYGVSRSVIRETVRVLESMGLVSSKRRVGVLVLPPSEWNLYDPQVIR
                     WRLASSGRIAQLRSLTELRTAIEPEAARLAATRSPLSNASDLMGLAGQLWAAGEAGEL
                     EYFLSLDIQFHRLVLVSSGNEMFAKLNTVVGEILTGRTNYGLMPRHPHQEALQLHVDV
                     ASAIQRGAPADAQAAMLGIMQRTTQEMTHLWDSPHQNADFLKNP"
     gene            1825..2790
                     /locus_tag="VFQ54_10225"
     CDS             1825..2790
                     /locus_tag="VFQ54_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0165410.1"
                     /translation="MTTTTIPSDSIDVAPAKPAAGPRRRGRIRNRRRLAGVLFVLPAA
                     LFIFTFMLYPLIYSGYMSLTRYNFVYDATPTFIAFGNYIKAMTDPLFVASITNTFVFG
                     VSYFVIVMIAGLGIALLLFQKIRFNAFFRSAIFVPIVVPLSLACLIFLWILQPNYGFL
                     NYFLGDVLHLPQLTQPWLSNGPTAMVAIVVVALWASVGFVTILFLAGLQGISNEVLEA
                     AEMDGARGLKKIVYIIVPSLRQTFVVAGTWAIIHGFKVFVEPMVMTAGGPRTATLVLY
                     QNVYQTAFTFFDMGYASAMGYILGAIILIFVGINFWFGRTKKDER"
     gene            2833..3636
                     /locus_tag="VFQ54_10230"
     CDS             2833..3636
                     /locus_tag="VFQ54_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0165411.1"
                     /translation="MTTQLVLLVLAVLFLVPLAYIVFSSFKSPGEALQVPQTFLPRHW
                     SLANYATVFNGGQFGAYFWNSIVITVGGVLITAVVASLAGYGFAKLPFRGQGVLLTLI
                     LATLTIPLVVFLVPMFLMEDGTGLLNTQIGLILPNVALHLPFTILIMRAAFLAIPKEV
                     EESATLDGAGAFRAWWAVMLPMARNGVVLCLIVTTYETWGEYTLAKTLAIDPPAMPLT
                     VGLTLLKGEVWDFGTLSAVIVLAILPPIIVFAFFQRHIVAGLAQGAIKG"
     gene            3818..>4031
                     /locus_tag="VFQ54_10235"
     CDS             3818..>4031
                     /locus_tag="VFQ54_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165412.1"
                     /translation="MLNVGVQPWMVTKGKFDVVVKNFEKKNPGIKVNLVQYADNQTLA
                     NYALQWAQKKTSVDVVVVGTCSQAVQF"
BASE COUNT          727 a         1306 c         1215 g          783 t
ORIGIN      
        1 cggcccatgt cgccaacacc tggcagacga tccagaccgt gcgggccggt gcccggcatt
       61 gaagcgtcgg gaaccgagta ttcagcgggt ggtgctcagc gcgccgctct cgacgctgag
      121 cctcgcgatc acctcgccga cgatctgctc cggcgggagc atgatgtcga ctcggattcc
      181 gagctcgtcc ggccccagcg gctcgaggct cgcgagctgc gattcgagca gcgacgacgg
      241 catgaacgcg tggtttcgac gggctatcct ctcccggatg acgtcgctcg gaccatcgag
      301 aaacaccacg aacaggccgg gggagtagct cctgagcacg tcgcggtatt gccgcttgag
      361 cgcagagcac gccacgatct cggttgatcc agacgatgcg ctcgcggcca ggtgcttgcc
      421 gatggcctcg agccatggga gacgctcgga atcgtcgaga ggcgctcccg cggccatctt
      481 cgcgatgttg ctcgcggaat ggaaccggtc gccgtccgtg aaacccgcgt cgagcttctt
      541 ggcaaggaga gcaccgatgg tgctcttgcc cgatccggac acacccatta caacgatcga
      601 ccgcacggcc atgaattgtc ttacccctac ttgttcggtg tcaaacagtt cgagtcgcgc
      661 cagcggaccc gtggtgcgat ggcacctcat tcgggtgatc cgcgctccga ggaggccaga
      721 tctgttctaa cggatgactt tatgttaata agcatactta tgcaaccacc agcggcagta
      781 ccatatcatt taccaaatga acgagccact caatgaagat gcaggcccgg atgacggctc
      841 ggatgaattc gcgccgagcg gccgcgggtt gcacgccaag gtcctcgatc aactcggact
      901 ggcggtctgc ggtcaggagt tccccaccaa cacggtcatg acgatcgagg acatcgagcg
      961 gcggtacggg gtgtcccgct cagtcatccg ggagacggtg cgcgtgctcg agtcgatggg
     1021 cctcgtctcc tcgaagcgcc gtgtcggcgt gctcgtcctg ccgccgagcg agtggaacct
     1081 ctacgacccg caggtgatcc ggtggaggct ggcttcatcg gggcggatcg cccagctcag
     1141 atccctcacc gagctgcgga ctgccatcga gccggaggcg gcgcggctgg ccgccactcg
     1201 ctcgccgctg tccaacgcca gtgatctcat gggactcgcg ggccagctct gggcggcggg
     1261 cgaggccggc gagctcgaat acttcctctc tctcgacatc cagttccacc ggctggtgct
     1321 tgtgagctcg ggcaatgaga tgttcgccaa gctcaacacc gtggtcggcg agatcctcac
     1381 cggacgcacc aactacgggc tgatgccccg gcatccgcat caggaggcgc tgcagctgca
     1441 cgtcgacgtc gccagcgcga tccagcgcgg agcgccggcc gacgcacagg cggccatgct
     1501 cggcatcatg cagcgcacca cccaggagat gacccacctc tgggattcgc cccatcagaa
     1561 cgcggacttc ctcaaaaacc cgtgatcacg tccctcgggg gacggtttgt cgctggttgt
     1621 ggcgactgat aatacggata ccgtttgcaa tttgcataag tatgcctttt ggtcatagag
     1681 tcgcggcaag gaaagggcct acagcgtcgc aagcgacgcg atggtttctg ccgcatgaat
     1741 ctcaaggaaa ctcgaggggg atttcttggc aagtgcaccg tcaccacgtc agggactcct
     1801 tcccgcctgc agcgagggtt tgcgatgacc acgaccacga ttccatcgga ctccatcgat
     1861 gttgcgccgg ccaagccggc ggctggtccg cgacgtcggg ggcggatccg caatcgcagg
     1921 cgtctcgcgg gcgtgctctt cgtcctgccc gccgcgctct tcatcttcac gttcatgctc
     1981 tacccgctca tctactcggg ctacatgagc ttgacgaggt acaacttcgt ctacgacgcg
     2041 acgcccacct tcatcgcctt cggcaactac atcaaggcga tgacggaccc gcttttcgtg
     2101 gcgagcatca ccaacacctt cgtcttcggg gtcagctact tcgtgatcgt gatgatcgcg
     2161 ggactcggga tcgcactcct gctcttccag aagatccggt tcaacgcgtt cttcaggagc
     2221 gccatcttcg tccccatcgt cgtgccgctg tccctcgcct gcctgatctt cctctggatc
     2281 cttcagccca actacgggtt cctcaactac ttcctcggtg acgtccttca ccttccgcaa
     2341 ctgacccagc cgtggctgag caacggcccg accgccatgg tggcgatcgt ggtggtcgca
     2401 ctgtgggcga gcgtggggtt cgtcaccatc ctgttcctcg cgggactgca gggaatctcg
     2461 aacgaggttc tcgaggcggc ggagatggat ggcgctcgcg ggctcaagaa gatcgtctac
     2521 atcatcgttc cgagcctgcg gcagaccttc gtcgtcgcgg ggacctgggc gatcatccac
     2581 gggttcaagg tcttcgtcga acccatggtg atgacggcgg gagggccgcg cacggcgact
     2641 ctggtgctct accagaacgt ctatcaaacg gctttcacct tcttcgacat ggggtacgcc
     2701 tcggcgatgg gatacatcct cggggcgatc atcctcatct tcgtcggcat caacttctgg
     2761 ttcggccgaa ccaaaaaaga cgagagataa aaagggagcg ccgcagccat gaaaggaact
     2821 ttcgcttcga ggttgacgac ccagcttgtg ctgcttgtgc tcgccgtcct cttcctggtg
     2881 cctctcgcct acatcgtctt cagctcgttc aagagcccgg gcgaagccct tcaggtgccg
     2941 cagaccttcc tgccgagaca ctggtcgctc gccaactatg cgaccgtctt caacggcggc
     3001 cagttcgggg cgtacttctg gaacagcatc gtcatcacgg tgggcggcgt gctcatcacg
     3061 gccgtcgtcg cctcattggc cggctacggg ttcgccaagc tgcccttccg cggccagggg
     3121 gtgcttctca ccctcatcct cgccaccctg accattcccc tggtcgtgtt cctggtcccc
     3181 atgttcctca tggaggacgg aaccgggctg ttgaacacgc agatcggcct gatcctgccc
     3241 aacgtcgccc tccacctccc gttcacgatt ctgatcatgc gggccgcgtt cctcgccata
     3301 ccgaaagagg tggaggaatc ggcgaccctc gacggcgccg gcgcgttccg cgcgtggtgg
     3361 gcggtcatgc tcccgatggc gaggaacggc gtggtcctct gcctgatcgt gaccacgtac
     3421 gagacctggg gcgagtacac cctcgccaag acgttggcga tcgaccctcc tgccatgccg
     3481 ctgaccgtcg ggctcacgct gctgaagggc gaggtctggg atttcggcac tctttcggcg
     3541 gtcatcgtgc ttgcgatcct cccgccgatc atcgtcttcg ccttcttcca gcgacacatc
     3601 gtcgccggcc tggctcaggg agccatcaag ggataagccg caccatccga ggcgtcatcg
     3661 tctcggcgta accccatcaa ggaagtaacc aacgaaaggc tagggaatga gaaggaaata
     3721 tcgcatcgca gcggcgatag tcgccgcagg cgccgtgctc gctggaacca tcggatgttc
     3781 gtcctcgacc acgacagcga gcggcaagga atcgtctgtg ctcaacgtgg gcgtccagcc
     3841 ttggatggtc accaagggca agttcgatgt cgtcgtgaag aacttcgaga agaagaaccc
     3901 cggcatcaag gtgaacctgg tgcagtacgc cgacaaccag acgctcgcca actacgcgct
     3961 gcagtgggcg cagaagaaga ccagcgtcga tgtcgtcgtg gtgggcacct gctcgcaggc
     4021 ggtgcagttc c
//
LOCUS       DASTMD010000692         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_868050, whole genome shotgun sequence.
ACCESSION   DASTMD010000692 DASTMD010000000
VERSION     DASTMD010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868050"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFQ54_10240"
     CDS             complement(<1..587)
                     /locus_tag="VFQ54_10240"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEU0165413.1"
                     /translation="MLLAIIADDLTGAADAAATFATRGFATSIAFTAETASDLDVVVR
                     STESRDGDLESAVSAVDAATNALTRDSNGPTPAFIYKKIDSVLRGHPREEFFALLRVT
                     GERRALLTPALPSEGRRTVLGRHFVNGTPLEASSFGGPGVTSDLARLFRNDEFGGVRL
                     LDLATIRAGTGNIAGFLEANMDGIVIADAETDEDL"
     gene            complement(595..1620)
                     /gene="pdxA"
                     /locus_tag="VFQ54_10245"
     CDS             complement(595..1620)
                     /gene="pdxA"
                     /locus_tag="VFQ54_10245"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU0165414.1"
                     /translation="MAADYRPLLAITMGDPAGIGPEIILKALKHDEVYSRARPLVIGD
                     RRTLERAAAWVAASDLTYDVVVSPSEGRYQPGVISLLDLENAPADGYRIGEVSPEAGK
                     AAVEFVFQACDLALAGSVDAVVTAPLNKAAMHQAGFIYPGHTELLAERTGAEKVSMLL
                     VGPSLRVVHVSTHVALSEAIRRVTRDRVGDVITIAQEACIALGIAKPRIAVAGLNPHA
                     SEGGLFGNQEAEEIIPAIADARARGLDVSDPQPPDTVFLRASRGTWDIVVAMYHDQGH
                     IPMKLLAFDSGVNVSIGLPILRTSVDHGTAFDIAGTGKASEESMLAAIDVAIQLGAAR
                     PIASRAG"
BASE COUNT          297 a          555 c          552 g          291 t
ORIGIN      
        1 tggagatcct catccgtctc ggcgtcggct atcacgattc cgtccatgtt cgcttccagg
       61 aacccggcga tgttccctgt tccggcacga atcgtcgcga gatcgaggag ccggacgccg
      121 ccgaattcat cgttgcgaaa gagccgggcg aggtcagacg tgacgccggg accgccaaac
      181 gagctggcct ccaatggcgt cccgttgacg aagtgacgac ccaggaccgt gcgccggccc
      241 tcgctgggga gcgccggcgt caggagcgca cggcgttcac cggtcactcg caacagcgcg
      301 aagaattcct cgcgcggatg tccccgcagc acggagtcga tcttcttgta gatgaacgcc
      361 ggagtaggtc cgttcgaatc gcgagtcagc gcgttcgtcg cagcgtcgac ggcgctcacc
      421 gccgattcga gatccccgtc gcggctctcg gtcgagcgga cgacgacatc aagatcggaa
      481 gcggtttccg cggtgaaggc gatcgacgtc gcgaagccac gcgtggcgaa ggtagcagcc
      541 gcatcagccg cgcccgtcag atcgtcggcg atgatcgcga gaagcatcag gtcgctagcc
      601 ggcgcgactg gcgatcggcc gcgccgcgcc gagctggatc gcgacatcga tcgcggcgag
      661 catgctttcc tcgctcgcct tgccggttcc cgcgatgtcg aaggccgtgc catgatccac
      721 ggatgtgcgg aggatcggga gtccgatact gacgtttacc ccggagtcga acgcgaggag
      781 cttcatcggg atatgtccct ggtcgtggta catcgcgacg acgatgtccc aggtcccgcg
      841 cgatgcacgg aggaagacgg tgtccggcgg ctgcggatcg ctcacgtcga gcccgcgggc
      901 acgcgcgtcc gcgatcgccg ggatgatctc ttccgcttcc tggttgccaa agagcccacc
      961 ctcgctcgcg tgcggattga gaccggcgac ggcgatccgg ggtttcgcaa tacccagcgc
     1021 gatacaggcc tcctgcgcga tcgtgatcac gtcacccacc cgatccctcg tcacacggcg
     1081 gatcgcctcg ctcagggcaa cgtgggtgct cacgtggacc acgcggagcg agggaccgac
     1141 gagcagcatg ctgaccttct cggcgccggt tcgctcggcg agcagttccg tgtgtccggg
     1201 atagatgaag cctgcctgat gcatcgccgc cttgttcagg ggagcggtga cgacggcatc
     1261 gacgctgccc gccagcgcca gatcgcacgc ctggaagacg aactcgaccg cggcttttcc
     1321 tgcttccggt gagacctcgc caatcctgta gccatcggct ggtgcattct ccagatcgag
     1381 cagcgagatg acacccggct gatagcggcc ctccgacggg gaaacgacta catcataggt
     1441 gagatcgctg gccgcgaccc acgccgccgc ccgctcgagc gtccgccgat cgccgatcac
     1501 gagcggtcgc gcacgcgaat agacctcatc gtgtttcaac gccttcagaa tgatctcggg
     1561 tccaatgccc gcgggatcgc ccatcgtgat ggcgagaagt ggtcggtagt ccgctgccat
     1621 ggaacgtcgc cccttgtacg gccctcgaaa tgatcggatg catgggctgg agtgtatgaa
     1681 cgcctcgggc gactg
//
LOCUS       DASTMD010000693         1744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2831931, whole genome shotgun sequence.
ACCESSION   DASTMD010000693 DASTMD010000000
VERSION     DASTMD010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2831931"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..703)
                     /locus_tag="VFQ54_10250"
     CDS             complement(77..703)
                     /locus_tag="VFQ54_10250"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEU0165415.1"
                     /translation="MDDRARGLQFHVEYPERALNRVSTLLRLIWVIPIAIVLGAVSGG
                     SWQWSSGGEQTAVAAGGLLFFAPLLLIVFRQKYPRWWFDWNLELLRFTNRVAVYLCLM
                     DDRYPSTDEQQSVALDFPYPDVPRDLNRWLPIVKWLLAIPHYIALFFLDILLVIAVIV
                     AWFAILFTGKYPRDLFSFAEGVLRWNNRVVAYALILVTDDYPPFKLSA"
     gene            1079..>1744
                     /locus_tag="VFQ54_10255"
     CDS             1079..>1744
                     /locus_tag="VFQ54_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEU0165416.1"
                     /translation="MGSELLLGHITDTNATFLAQELTANGIELIHVTSTGDDLAELTA
                     TLKRALEQADVVICSGGIGPTDDDLTREAIAAVVGETPTIDPDLLTTLKGFFAGRGMD
                     MPERNAKQAWTIPSSDVLPNPVGTAPGWFVTVAGDEPKYIVTMPGVPREMFRMWSEQA
                     LPRIRERSGQSIIDTIVIKTIGIGESAAEQEIHDLILASDPIVATYAKDDGVHVMVSA
                     VGND"
BASE COUNT          369 a          551 c          520 g          304 t
ORIGIN      
        1 agtccccaca gaaaatcctg ccaccacatc aagcattccc ttctcggcga tcagacgtat
       61 tcagacgccg gcgacgtcac gccgagagtt tgaacggagg atagtcgtcg gtcacgagga
      121 tcagcgcata ggcgaccacg cgattgttcc agcggaggac tccctccgcg aagctaaaga
      181 ggtcgcgcgg gtatttgccg gtgaagagga tcgcgaacca ggcgacgatc accgcgatca
      241 ccagcaggat gtccaggaag aaaagcgcga tgtagtgtgg aatcgcgagc agccacttga
      301 cgattggcaa ccacctgttc aggtcgcgag gcacgtcggg atacgggaag tccagcgcca
      361 cactctgctg ctcgtcggtc gacggatagc ggtcgtccat caggcagaga tagacggcga
      421 cgcgattggt gaagcgaagg agctccaggt tccagtcgaa ccaccagcgt ggatacttct
      481 ggcgaaagac gatcaggagc aacggcgcga agaagagcaa gccaccggcg gcaacggccg
      541 tctgctcacc gccggacgac cactgccacg atccgccgga aacggcgccg agcacgatgg
      601 cgatcgggat cacccagatc agcctgagca gggtcgaaac gcggttcagc gcgcgctcgg
      661 gatattcgac gtggaattgc aatcctcttg ctcgatcgtc catctccaga tcctccctcg
      721 gctttctctc gaacctgctc gccggacgcg acgcccggac agggtcggca gcactactcc
      781 gtgagcccgt ctcgtcgttc catcgctctc atccgtttcg tccgcctgcc ttactctatc
      841 gggttcgcta tgcagaactc gtgtcttctc cggtcaatac atgtcgaaaa gacaacaaaa
      901 tagctcaaca cggtcggtgc gagctttcgc gacgtcgtgt tgagcccgcc gcatgccgac
      961 ggcgtagtgc tgttcacatt ctggtcgctg gctataatga cgcagctaac agccgacccg
     1021 acttccgtga ggaatcacta gtgccgaaag gcggaccatg cgcgcctata tttgctagat
     1081 cggatccgaa cttctgctcg gtcatatcac cgacacgaat gccaccttcc tcgcgcaaga
     1141 actcaccgcc aacggcatcg aactcatcca cgtcacgagc accggcgacg atcttgccga
     1201 gctgacagcg acgctcaagc gggcgctgga acaggccgac gtcgtgatct gttctggcgg
     1261 catcgggccg acggacgacg atctcacccg tgaagcgatc gccgcggtcg tcggcgagac
     1321 gcccaccatc gatcccgatc tgctgacgac gctgaaggga ttctttgccg gtcgcggcat
     1381 ggatatgccg gaacgcaacg ccaagcaggc gtggacgatc ccatcctccg acgtgctgcc
     1441 gaatccggtg ggcaccgcgc ccggctggtt cgtcaccgtc gccggcgacg agcccaagta
     1501 catcgtgacg atgccgggcg tgccccggga gatgttccgg atgtggtccg agcaagcgct
     1561 cccccggatc cgcgagcggt ccggccagag catcatcgac acgatcgtga tcaagacgat
     1621 cggtatcggt gagtctgccg cggagcagga aatccatgac ctgatcctgg ccagcgaccc
     1681 gatcgttgcc acgtatgcga aggatgacgg ggtccacgtg atggtctcag ccgtcggcaa
     1741 cgac
//
LOCUS       DASTMD010000694         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_776615, whole genome shotgun sequence.
ACCESSION   DASTMD010000694 DASTMD010000000
VERSION     DASTMD010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776615"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFQ54_10260"
     CDS             complement(<1..674)
                     /locus_tag="VFQ54_10260"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0165417.1"
                     /translation="MPLITFDFHNTLAHCDAWFELEVRTLPSVVLAAASGIVLDDETR
                     VSVDAAYRRVREQVMATGIELDALASVLATYDALGIEVDREAATAAIDAAMRHALHDL
                     QPVDGAVALVTQLHEDGYRLGIVSSAVYHPFLEWALAAFDIAHCFEFVVTSASAGYYK
                     SSPAIYHHALGLASMRPHEAVHIGDSARWDVTTAASIGIGTVLLRTARIDRFANAADL
                     LPPDLTL"
     gene            complement(767..2011)
                     /locus_tag="VFQ54_10265"
     CDS             complement(767..2011)
                     /locus_tag="VFQ54_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU0165418.1"
                     /translation="MAYSVGFLPHMAADDWFVAASLVILLFAVEHFDVTISVASGPFS
                     MSVGATIAMAAALHFNVPVAVFVVLLAHVLDSAVALRQLMKSMTNIGTYVCSTFVASA
                     IYHSLADTSVSPLANATNILYAVLASAAFVALSTSILALIVGPIIGVPFLQLWQQNIR
                     ATALETVGLPALGGIVAVLANENAAAVLLVGFPLMAPQLAYKALQEARVSVRATIETL
                     IDTLEKRDRSTVDHSRRVAEYAEAIVEQMGSIPHQATDVILWAARVHDLGKIGVRDST
                     LFKAGPLTPRERIEMQAHPTLGADIVAKLAQSDPVAVIIRHHHERWDGKGYPDGLAGE
                     AIPLGSRIIAVADTFEAMTADRPYRPALPISVAYEEILRNSGTQFDPGVVRAFGRAFG
                     PAESRGQAGQVQSQPQSHLTTA"
     gene            3380..>3621
                     /locus_tag="VFQ54_10270"
     CDS             3380..>3621
                     /locus_tag="VFQ54_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HEU0165419.1"
                     /translation="MPESSLIDLAIAKTEKYASRESGDTVELIERPSGGFSIVMIDGQ
                     GSGRAAKTLSLMLSSKAVALVKEGVRDGVVARATHD"
BASE COUNT          792 a         1022 c         1160 g          647 t
ORIGIN      
        1 agtgtgaggt cgggggggag caggtccgcg gcgttggcga agcggtcgat gcgcgccgtc
       61 ctgagcagga ccgtgccgat cccgatcgac gctgcggtcg tcacgtccca gcgagccgag
      121 tcgccgatat gtaccgcctc atggggacgc atcgatgcga ggccaagtgc gtggtggtaa
      181 atcgccgggc tcgacttgta gtagcccgct gacgcggagg tcaccacgaa ttcgaagcaa
      241 tgggcgatat cgaacgccgc gagcgcccat tccaggaacg ggtgatagac cgcgctgctc
      301 acgatgccca ggcgatagcc atcctcatga agttgcgtga cgagagcgac cgcaccgtcg
      361 accggttgaa ggtcgtggag ggcgtggcgc atcgccgcat cgatggcggc ggtcgccgcc
      421 tcgcgatcga cctcgattcc gagggcgtcg tatgtcgcga gcaccgaggc cagcgcatcg
      481 agctcgattc ccgtcgccat cacctgctcg cggacgcggc gatacgcggc gtcgaccgag
      541 acccgggtct cgtcgtccag gacgatgccg gaagccgccg cgaggacaac cgaaggaagc
      601 gttcgcacct cgagctcgaa ccaggcgtcg caatgggcaa gcgtgttgtg gaagtcgaag
      661 gtgatcagcg gcacagggaa actaccttgg cgtgaaagaa gcgggaggtg tcacgccgga
      721 aggatacctg agcgggaggc aagtcaaccg ggaccgtgcc ttgccgtcag gccgtcgtga
      781 gatgactctg gggttgcgat tggacctgac ccgcctgccc acggctctcg gcggggccga
      841 aggcgcgacc gaaggctcgc acgacgccgg gatcgaactg cgtgccgctg ttgcggagga
      901 tctcctcata ggcgacggag atcggcaacg ccggccggta tggccggtcg gcggtcatcg
      961 cttcgaatgt gtcggcaacg gcgatgatgc gggaaccgag ggggatcgct tctccggcga
     1021 ggccatccgg gtaccccttg ccatcccaac gctcgtgatg gtggcggatg atcacggcga
     1081 ccggatccga ctgcgcgagc ttggcgacga tgtccgcgcc gagggtcggg tgcgcctgca
     1141 tctcgatccg ctcgcgcggc gtcagcggtc ccgccttgaa gagcgtcgag tccctgacgc
     1201 cgatctttcc cagatcgtga acgcgtgcgg cccagaggat gacatcggtc gcctggtggg
     1261 ggatcgatcc catctgctcg acgatcgctt ccgcgtattc ggcgactctt cgggaatggt
     1321 ccacggtgga tcgatcgcgt ttctcgaggg tgtcgatcag ggtttcgatc gtggcgcgaa
     1381 cgctgacgcg tgcttcctgg agggccttgt aggcgagctg gggcgccatc agcgggaagc
     1441 cgacgaggag gaccgctgcc gcgttctcgt ttgcgaggac ggcgacgatc ccaccgaggg
     1501 cgggaaggcc gacggtttcc agcgctgtcg cgcggatgtt ctgctgccag agctggagga
     1561 atggaacgcc gatgatcggg ccgacgatca gcgcgaggat cgatgtgctc aacgcaacga
     1621 aggcggcaga ggcgagaacg gcatacaaga tgttggtggc attcgccagc ggcgagacgg
     1681 acgtgtccgc gaggctgtgg tagatggcgg atgcgacgaa ggtggagcag acgtaggtgc
     1741 cgatgttcgt catcgatttc atcagctgtc gcaaggcgac ggcggaatcg agaacgtggg
     1801 cgaggaggac gacgaagacc gcgacgggga cgttgaagtg gagagcggcg gccattgcga
     1861 tcgtggcgcc gacggacatc gagaagggtc cggaagcgac agagatcgtg acgtcgaagt
     1921 gttcaaccgc gaagaggagg atgacgagag aggcggcaac aaaccagtcg tccgcagcca
     1981 tatgcggaag aaagccaaca ctatacgcca ccgctccaga agtcgcgatg acgagcagga
     2041 gaagccagaa tcgggttctc aacgattctc ccaacattcc ggtggggtgc cctataccga
     2101 tattgattag aagacatgga acggcgcgaa tcgcacgtga ggtgggctgc cacggccacc
     2161 tgatgtgtac ccccgtacca agccagatta acgcattcgt ccggcaaaga caataaagac
     2221 acaggaccat cagataaagg gcgctattca cccccaggac ctatgggcta tccgactttc
     2281 gaaggtccta gcttgccgtg caaggaacct atgacctgtt tcccgctata tatccatgcc
     2341 attcagcaag atcaaacgat ttcgtactgt cccaaaggta tgtggtcagc aaaagagaat
     2401 gtgacgggta tcgccgtcat gaccattccc gagggggtca aatgcgacaa actgggcatt
     2461 cccaacgggg tatcgattcg acgatggtaa taacaagtcg cggaaggccc gggtgacggt
     2521 accagggcta gacccacttc aagccgttca tgacaatctc ctgacgttga tgtgcaaatc
     2581 gaccaaaaac gagacctaga cccacttcag accgctcatc gtcttctcct ggctgtgaga
     2641 tgcgaaccga ccgagacgaa aacctagacc cacttcaagc cgctcatgac aacctcccgc
     2701 tgtgttgagt gcgaaccaac cggaaacaag acctagaccc acttcaaacc gctcatgaca
     2761 acctccctgg actgagattc aaattcgacg acgcgctcga ctagacccac ttcagaccgc
     2821 tcattgaacc ctccatgctg ttgagtgcga acgaaccgga gacgaaacct agacccactt
     2881 cagaccgctc atgaaaccct ccccgctgcg aaatgccgac gttccggaag cagaatctag
     2941 acccacttca agccgctcat gacaacctcc tgatcgttcg agatgaggga acaatgccga
     3001 attacaactg ggttcaactg gaaaaagctg tgattgacgc atcttggcgg atacggaaac
     3061 tttggagata acgtcgatca atcgccgatc aacctgagga agatgatagt ttagccggga
     3121 tcgattgtca ataccaaagt gaatatggat tttggaacgc actctggcag gttggggcac
     3181 aggcgtgttt ttgtgttgcg gtcgtcccgt ggtttttctc atttacatga gccctcgtgc
     3241 gtgattctgg catcgttcca accggaatag gtccccaacg cattcaacgt gacattcaca
     3301 atcggtcgcg gaccgacctg gaccgactgc ggatacaatg cgggggtttg tcgatgtgtt
     3361 tccgtatggg agagcgttcg tgccagagtc cagcctgatc gatctcgcca tcgcgaagac
     3421 ggagaagtac gcaagccgcg agagtgggga taccgtcgaa ctcatcgagc gtccgtccgg
     3481 gggcttttcg atcgtcatga tcgacggcca gggcagcggt cgcgccgcga aaacgctcag
     3541 cctgatgctc tccagcaaag cggtcgcgct ggtgaaggaa ggcgtccgcg atggggttgt
     3601 tgcgagggcg acgcacgact a
//
LOCUS       DASTMD010000695         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3190510, whole genome shotgun sequence.
ACCESSION   DASTMD010000695 DASTMD010000000
VERSION     DASTMD010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3190510"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VFQ54_10275"
     CDS             complement(<1..738)
                     /locus_tag="VFQ54_10275"
                     /inference="COORDINATES: protein motif:HMM:NF021489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU0165420.1"
                     /translation="MNEPVERKRPSVLLRWLAVAGLALALAMAMLPIGRASAADTVTW
                     KNYDVTIDVQQNGDYHVSERQIVEFDGQFSHGFADLDLANLNSIDNVKVAVANTTSAT
                     PLASTFVDSSDYDEESGTYTTQQTGGSLEVDYGFDSTDYFGSEERLIVLEYDVSGALR
                     VYPDLVPPNQMLWFTAITKDVTDIANIDQASVTINLPKAVDAADIVANPVSPTTKGQS
                     YTWTKSDMTKGYDFEVRLQFPQITTATE"
     gene            1013..1453
                     /locus_tag="VFQ54_10280"
     CDS             1013..1453
                     /locus_tag="VFQ54_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEU0165421.1"
                     /translation="MLSQHGYRLTGPRAIIVETMLGYERPFTAEQLVSELRENERPDA
                     KRPIGRATVYRTLEILAAVDVLTRLIQPDGHPAYIWDSLGHRHHLVCSNCGTAVAFTA
                     CPIDELVRTLTKDTDFQIHDHMLEVFGLCPDCQAKNLIAARLQA"
     gene            1575..2402
                     /locus_tag="VFQ54_10285"
     CDS             1575..2402
                     /locus_tag="VFQ54_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU0165422.1"
                     /translation="MTPEPAAIGIPVGNDRAPIITTDKLTVHYENRPALTDVSIAVHR
                     SETLSILGPNGAGKSTLLKVIAGMLPPSHGSVAMDGVPLKGTNPRITYVPQRSGADWN
                     FPISVIEVVLLGLSRKTPRWRGFSGADRERALHALAEVRMEHLAKVQIGSLSGGQQQR
                     VFLARALLQCGDVLLLDEPFAGVDVPTQELFVELFRNLSSRGTAIVYATHDLPQAFQN
                     SDRVMMVNRRLVAVGTPDEVMNEHFLHETFGGNVIVLPSSVQIVDTEDAPAPEVALR"
     gene            2399..>3128
                     /locus_tag="VFQ54_10290"
     CDS             2399..>3128
                     /locus_tag="VFQ54_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU0165423.1"
                     /translation="MIDSLTEPWQYTFMQHAFIAIVLVGVICGVTGVFVTLRGLAFLG
                     DALSHAIFPGVVIAFISGGNFLLGALVAAVIVSLGIGAVSQSGRISNDTAIGVLFAGA
                     FALGVAMLSAQKSYARDLNAFLLGSILGVSTHDLYLIGAVGLIVLAVVYWFRRELTTV
                     AFDRTFARASGLNLWFYDQLFLTMLAFTIVISLQTVGNILVLAMLVTPAATARLLTDR
                     MQVMVWMSAVIGAACGVIGLYISYY"
BASE COUNT          514 a         1050 c          929 g          635 t
ORIGIN      
        1 ctccgtcgcc gtcgtgatct gcgggaactg caggcgaacc tcgaagtcgt agcccttcgt
       61 catgtccgac ttcgtccagg tatagctctg gcctttcgtc gtcgggctca ccggattcgc
      121 gacgatatcg gccgcatcga ccgccttcgg caggttgatc gtcaccgacg cctgatcgat
      181 gttggcgata tccgtgacgt ccttggtgat cgcagtaaac cacaacattt gattgggcgg
      241 gaccagatcc ggatagacgc gcagcgcgcc gctcacgtcg tattccagga cgatcagtcg
      301 ttcttctgag ccgaagtaat cggtcgagtc gaagccgtaa tccacctcca gcgagccacc
      361 ggtctgctgc gtggtgtagg tgccggattc ttcgtcgtag tcggaggagt cgacgaacgt
      421 cgatgccagc ggcgtcgcac tggttgtgtt cgctaccgcg accttcacgt tgtcgatcga
      481 gttcaggttg gcgagatcga ggtcggcgaa cccgtggctg aactgaccgt cgaactccac
      541 gatctgccgc tcggagacgt gatagtcgcc gttctgctgg acatcgatcg tgacgtcgta
      601 gttcttccag gtgaccgtat cggccgcgga cgctcgtccg atcggcagca tcgccatggc
      661 gagggccagt gcgagcccgg cgactgccag ccatcgcagc agcacggagg gtctcttccg
      721 ctcgacaggt tcgttcatcg gcacgctgcc ctcatcatgt tcggtcgaac agtcgttgtt
      781 gcacgtacgt cgcaaatagt tgtctttggc ctcgcgatca gagtatcgca tggatatcga
      841 cgtccatcgg gcgtctttcg ttgcatggaa gttatagaat agatgcagag attactgccg
      901 aggtcggcag tcgtcgttcc gcacagcaca ggacgcgagg cctcgcccaa acatggtcat
      961 gacagctccc gaaccccaaa cttccccatt gacgcgcgtc gcgatcgaga acgtgctttc
     1021 gcagcacggc tatcgattga ccgggccccg cgccatcatc gtcgagacga tgctcggtta
     1081 cgagcggcca ttcaccgccg agcaactcgt ctccgaactg cgcgagaacg aacgtcccga
     1141 tgcgaagcgc ccgatcggcc gggcgacggt ctatcgcacg ctggaaatcc tcgccgcggt
     1201 cgatgtcctg acgcgcctca tccagccgga cggccatccc gcctacatct gggattcgct
     1261 cggtcaccgc caccacctgg tttgctcgaa ctgcggcacg gcggttgcct tcacggcctg
     1321 cccgatcgat gagctcgtgc gcacgctgac caaggacacc gatttccaga tccacgatca
     1381 catgctggaa gtcttcggcc tttgcccgga ttgccaggcg aagaacctca tcgctgcccg
     1441 cctccaggcc tgatcgccgc ttcttttgac acttcgtctc acccgtgcta ccctgacagg
     1501 cgcttcgttc gcgggttgac gccgtcgtca cgcgccacct ccacgtcttt ccgactgtca
     1561 ggtaacctcc gctcatgacc ccggaacctg cggcgatcgg catccctgtc ggcaacgacc
     1621 gcgctcccat tatcacgacc gacaagctga ccgttcacta cgagaatcgt ccggcactca
     1681 cggacgtatc gatcgcggtg catcgcagcg aaacgttgag catcctcggc ccgaatggtg
     1741 ccggcaagag cacgctcctg aaggtgatcg ccgggatgct gccgccgtcg catggctcgg
     1801 tcgcgatgga cggtgtcccc ctcaagggga ccaatccccg gatcacctac gtgccgcaac
     1861 gctccggggc ggactggaac tttccgatct cggtgatcga ggtcgtcctg ctcgggctca
     1921 gccgcaagac gccgcgctgg cgaggattct ccggcgcgga ccgcgagcgg gcgctgcacg
     1981 cgctggccga agtccggatg gagcatctcg cgaaggtcca gatcggctcg ctctccggtg
     2041 gtcagcagca gcgcgtcttc ctggcgcggg cgctgctcca gtgcggtgat gtactgctcc
     2101 tcgatgagcc cttcgctggt gtcgatgtgc cgacccagga actcttcgtc gaactgttcc
     2161 gcaacctgag ctcccgcggg acggcgatcg tctacgcgac ccacgatctt ccccaggcgt
     2221 tccagaactc cgaccgcgtg atgatggtca accgtcggct cgtcgccgtc gggacacccg
     2281 acgaggtgat gaacgagcac ttcctgcacg agaccttcgg tggcaacgtg atcgtccttc
     2341 cgtcgtcggt ccagatcgtc gacacggagg acgcgccggc cccagaggtc gcgctgcgat
     2401 gatcgatagc ctgaccgaac cctggcaata caccttcatg cagcacgcct tcatcgcgat
     2461 cgtcctcgtc ggcgtgatct gcggcgtgac gggagtcttc gtgacgctca ggggactcgc
     2521 ctttctcggc gacgccctct cgcatgcgat cttcccggga gtcgtgatcg ccttcatctc
     2581 cggcggcaac ttcctgctcg gcgccctggt cgcggcggtc attgtctcgc tgggaatcgg
     2641 cgcggtcagc cagagcggtc gcatcagcaa cgacaccgcg atcggcgtcc tcttcgcggg
     2701 cgccttcgcc ctcggcgtcg cgatgctctc cgcccagaag tcctacgcgc gggacctcaa
     2761 cgccttcctg ctcggctcga tcctcggcgt ctcgacgcac gatctctatc tgatcggcgc
     2821 cgtcggcctg atcgtgctcg cggtcgtcta ctggttccga cgcgaactga cgacggtcgc
     2881 cttcgatcgc accttcgcac gagccagcgg gctcaacctc tggttctacg atcagctctt
     2941 cctgacgatg ctggccttca cgatcgtgat ctccctgcag acggtcggca acatcctcgt
     3001 gctcgcgatg ctggtgacgc cggccgcgac ggcgcgcctg ctcaccgatc gaatgcaggt
     3061 gatggtctgg atgtcggccg tgatcggtgc ggcgtgcggc gtgatcgggc tctacatctc
     3121 gtactacc
//
LOCUS       DASTMD010000696         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1045866, whole genome shotgun sequence.
ACCESSION   DASTMD010000696 DASTMD010000000
VERSION     DASTMD010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045866"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VFQ54_10295"
     CDS             complement(<1..1058)
                     /locus_tag="VFQ54_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0165424.1"
                     /translation="MSSNDLTRLTAAESRKLLDEREISSVELTDAYLGRIDAIDPAVH
                     AYLHVMSDVARGQATDADRRIASGENVGALTGIPIALKDILVTTDAPTTAASRILEGY
                     VSPYDGTVVRRLREQGAVFLGKTNTDEFAMGSSTENSAYGPTHNPWDLSRVPGGSSGG
                     SAASVAAREATISLGSDTGGSIRQPAGFCGIVGLKPTYGRVSRFGLIAFASSLDQIGP
                     FARTVEDAAIMLEAIAGKDPMDSTSVPLEVPRYRDSLRTDLKGIRVGVAHEYSVDGME
                     AGVDASIKAAIAQLEMLGAEIVDISLPHTEYALATYYITAPAEASANLSRFDGVKYGL
                     RLEEPSLLETYEKTRGEG"
     gene            complement(1055..1366)
                     /gene="gatC"
                     /locus_tag="VFQ54_10300"
     CDS             complement(1055..1366)
                     /gene="gatC"
                     /locus_tag="VFQ54_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU0165425.1"
                     /translation="MSLSRQDVDHVASLARLGLSDDEKETLRDQLSSILEHIAVLNAI
                     DTESIPPTAQVIALQNVLRDDEIRPSLSQEQVLQNAPRSRDGFFEVRAVMASGSDGES
                     A"
     gene            complement(1444..>2769)
                     /gene="nagA"
                     /locus_tag="VFQ54_10305"
     CDS             complement(1444..>2769)
                     /gene="nagA"
                     /locus_tag="VFQ54_10305"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU0165426.1"
                     /translation="DGACGLNRRLEDKCGLGERTDRRDRGDPSARRDRTCPRAGDSGR
                     TLHRQVIPGSQQVMTTSMFAIRGDLLLGSHLAPGAVVVTDGVISGIERGEVSDGKLPP
                     TVIDAAIVSPGMIDLQVNGAIGREVGTDARDIEAISAWMPSTGVTSWLPTIVTAGADY
                     YPLVFAAWDELDQSIGATPLGLHLEGPFLSPRRKGAHQLRFIEAASDELFTSWLDQVS
                     VKLVTLAPERAGGHGRIRSLVARGILTSLGHTDATYEEFVAGVDQGARMATHLFNAMS
                     PMHHRQPGAMIATMTDERITCGLIPDGVHSHPATVRLAINAMGIDRIAVVSDMMSACG
                     FGPGTYGIGGQEVTVDETSARLADGTLAGSILTMDQAIRNLVAWSDATVGEALHMATA
                     VPAGLLRDVDRGALRIGARADITAWNGGLGIQETWVGGSSVYTATPAGR"
BASE COUNT          467 a          894 c          939 g          469 t
ORIGIN      
        1 aagccctcgc cgcgcgtctt ctcgtaggtc tcgaggagcg acggctcttc gaggcgcaac
       61 ccgtatttca cgccgtcgaa ccgggagagg ttcgcgctgg cctcggctgg cgccgtgatg
      121 taataggtcg cgagggcgta ttccgtgtgc ggcaacgaga tgtcgacgat ctcggcgcca
      181 agcatctcca gctgggcaat cgcagccttg atcgaggcat cgactccggc ttccatgccg
      241 tccacgctgt attcgtgagc aacaccgacg cggatgccct tgaggtccgt ccgcagcgaa
      301 tcgcggtagc gcggaacctc aagcggaacc gaagtcgaat ccattggatc cttgccggcg
      361 atcgcctcga gcatgatcgc ggcgtcctcg acggtccgag cgaacggccc gatctggtcg
      421 aggctgctgg caaaggcgat caggccgaag cgcgagacgc gaccgtaggt cggcttgagg
      481 ccgacgatgc cgcagaagcc ggcgggctgg cgaatcgatc caccggtgtc ggagccgagc
      541 gagatcgtcg cctcgcgggc agcgaccgac gcggcggagc caccactgga accgcccggc
      601 acgcgggaga ggtcccacgg attgtgggtc ggaccgtagg ccgagttctc ggtggatgat
      661 cccatcgcga actcgtccgt gttcgtcttg ccgaggaaga cggcgccttg ctcgcgcaag
      721 cggcggacga ccgtgccgtc atatggggat acatatccct caagaatgcg ggatgccgcc
      781 gtggtcggcg catcggtcgt gacgaggatg tccttgagcg cgatcgggat gccggtcagc
      841 gcaccgacgt tctcgccgga cgctatacgg cgatcggcgt ctgtcgcctg gcccctcgcc
      901 acgtcggaca tgacgtggag gtaggcatgc acggcgggat cgatcgcatc gatccgtccg
      961 agataggcgt cggtcagctc gaccgacgag atctcccgtt cgtcgagcag cttgcgcgat
     1021 tccgccgcgg tgaggcgtgt gaggtcgttg ctgctcatgc gctctccccg tcggatccac
     1081 tcgccatcac ggcgcgcacc tcgaagaagc cgtcacgcga tcgcggcgca ttctggagca
     1141 cctgctcctg gctcagcgac ggacgaatct cgtcgtcgcg aaggacgttc tgaagcgcga
     1201 tcacctgcgc cgtgggcgga atggattccg tatcgatcgc gttgagcacg gcgatgtgtt
     1261 cgagaatcga cgagagctga tcgcgaagcg tctccttctc gtcgtcgctc aggccaagtc
     1321 gcgccagtga ggcgacgtga tcgacatcct gcctcgaaag actcatggtc tctcccaaat
     1381 gtaatgatga aatccaacga agggcgtctg gcgcctgctg gtcgcgagtc tatcagatcg
     1441 cgctcagcgt ccggcaggcg tcgccgtgta aaccgaggac cccccgaccc acgtctcctg
     1501 gatcccgaga ccgccgttcc aggccgtgat gtcggcgcgg gcgccgatcc tcagtgcgcc
     1561 gcgatcgacg tcacgaagca gccccgccgg aaccgcggtc gccatgtgga gcgcctcgcc
     1621 cacggtggcg tccgaccagg cgacgagatt gcgaatcgcc tggtccatgg tcaggatcga
     1681 accggcgagt gtcccgtccg cgagccgggc ggaggtctcg tcgaccgtga cctcctggcc
     1741 gccgatgccg tatgttcccg gtccgaaacc gcaggccgac atcatgtcgg aaacgacggc
     1801 gatgcggtcg attcccatcg cgttgatggc gaggcgcacg gtcgccgggt gggagtgcac
     1861 gccgtcggga atcaggccgc aggtgatgcg ctcgtccgtc atcgtcgcga tcatcgcgcc
     1921 cggctggcgg tgatgcatcg gcgacatcgc gttgaagaga tgggtcgcca ttcgcgcacc
     1981 ctggtcgacc ccggccacga actcctcgta cgtcgcgtcg gtgtgaccga ggctcgtgag
     2041 gatgccccgg gcgacgagac tgcggatacg accgtggcca ccggcccgct cgggcgcaag
     2101 cgtcacgagc ttgacggaca cctggtcgag ccaggacgtg aagagctcgt cacttgccgc
     2161 ctcgatgaag cggagttgat gcgcgccctt gcgtcgtggc gagaggaaag gtccttccag
     2221 atgcagtccg aggggcgttg ccccgatcga ctgatcgagc tcgtcccagg cggcgaagac
     2281 gaggggataa tagtccgcgc ccgccgtgac gattgtcggt aaccacgacg tgaccccggt
     2341 cgacggcatc cacgccgaga tcgcctcgat gtcgcgggcg tcggtaccga cttcccggcc
     2401 gatcgccccg ttcacctgga ggtcgatcat tcccggcgag acgatcgcgg cgtcgatcac
     2461 ggtcggtggc agcttgccgt cggaaacctc gccgcgctcg atgccggaga tcacgccgtc
     2521 ggtgacgacg acggcgcccg gcgcgagatg ggatccgagc aggagatcgc cgcggatggc
     2581 gaacatggat gtcgtcatga cttgttggct ccctggaatg acctggcgat ggagcgtgcg
     2641 gcctgaatcg ccggctcgag gacaagtgcg gtctcggcga gcggacggat cgccgcgatc
     2701 gcggcgatcc gtccgctcgc cgagaccgca cttgtcctcg agccggcgat tcaggccgca
     2761 cgctccatc
//
LOCUS       DASTMD010000697         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2832176, whole genome shotgun sequence.
ACCESSION   DASTMD010000697 DASTMD010000000
VERSION     DASTMD010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2832176"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..518
                     /locus_tag="VFQ54_10310"
     CDS             66..518
                     /locus_tag="VFQ54_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEU0165427.1"
                     /translation="MSSEGMDAVAIDPRRNRPIRILVAKPGLDGHDRGAKVLARAFRD
                     AGFEVIYTGLFQTPEMIANASLQEDVDVVGLSILSGAHLSLFPAIMEALRGVGRDDAL
                     VIAGGTIPDDDVPLVKEMGVAEVFGPGTRLQDAIAYVRDNAPIRTDLP"
     gene            520..1494
                     /gene="meaB"
                     /locus_tag="VFQ54_10315"
     CDS             520..1494
                     /gene="meaB"
                     /locus_tag="VFQ54_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU0165428.1"
                     /translation="MIDPTRPGLSARVLADDRRALARLLTYIENDDDIGLAAMDILYP
                     HTGKAHLIGITGPPGGGKSTLVNEMIRAYRAQGKSVAVIAVDPSSPLTGGATLGDRIR
                     MLEWHADPGVFIRSMASRRFGGGLAQNTLAVAHAFDAAGFDPIIIETVGTGQDEVAIA
                     NLAMTTLLVQVPGMGDSVQTLKAGALEIGDVLVVTKADRPEANDLVRDLRRLRTLTFE
                     PVVEDASVWQAPIVKTSSVRLEGIDDLLVAITAHREWLEASGRLESRRREIAVAEIAT
                     RVQTHLQRIARQQSTLDAGFGGMIDEVVARRLTPQRAAERLASRLSSK"
     gene            1621..>2224
                     /locus_tag="VFQ54_10320"
     CDS             1621..>2224
                     /locus_tag="VFQ54_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0165429.1"
                     /translation="MNSVYAALPTTIFEIVSETARKTGAVNLGQGFPDDPGPEDVRRK
                     AAEAVVDGWNQYPPMLGLPELRQAVASHYARHQGLDLDPSREIMVTSGATEAIAGALF
                     ALIEPGDEVILFPPVYDAYTPLVRRAGGIPVFVPLTPPDWEITREALEAAVTPKTRFL
                     LFNNPLNPAAKVFPRSELEILAAFCIEHDLIAICDEVWEHV"
BASE COUNT          369 a          728 c          738 g          389 t
ORIGIN      
        1 ggcgcggctt ccacacgtat ccggaaacgg cctagccggc cgccggtgag gattggagtg
       61 atcgtatgag ttccgaaggc atggacgccg tggcgatcga cccacgccgg aaccggccga
      121 ttcgcatact cgtcgcgaag ccgggtctgg acggacacga tcgcggggcg aaggtgctcg
      181 cccgggcatt ccgcgacgcg gggttcgagg tgatctacac tggtctcttc cagaccccgg
      241 agatgatcgc gaacgcctcg ctgcaggagg acgtcgacgt cgtcggcctg agcatcctct
      301 ccggcgccca cctctcgctc ttccccgcga tcatggaggc gctgcggggc gtcgggcgcg
      361 atgatgccct cgtgatcgcc ggtggcacga ttccggacga cgacgttccg ttggtcaagg
      421 agatgggcgt cgccgaggtc ttcggtccgg gaacccgcct gcaggacgcg atcgcttacg
      481 tccgcgacaa cgcgccgatc cgcacggatc tcccataacg tgatcgatcc cacccgaccc
      541 ggactctcgg cccgcgtcct ggccgacgat cggcgggcac tggcccgcct cctcacctac
      601 atcgagaacg acgacgatat cggactcgcg gcgatggaca tcctctatcc ccacaccggc
      661 aaggcacatc tgatcggcat cacggggcct cccggcggcg gcaagtcgac actcgtcaac
      721 gagatgatcc gcgcctatcg cgcccagggg aagtccgtcg ccgtgatcgc cgtcgatccg
      781 tcgagcccgc tcaccggcgg cgcgacgctc ggtgaccgga tcaggatgct cgaatggcat
      841 gccgatccgg gcgtcttcat ccggagcatg gcctcccgcc gttttggcgg cgggctcgcc
      901 cagaacacac ttgcggtcgc tcatgccttc gatgccgccg gcttcgatcc gatcatcatc
      961 gagacggtcg gcaccgggca ggatgaggtc gcgatcgcca acctcgcgat gacgacgctc
     1021 ctcgtccagg tgcccggcat gggagacagc gtgcagacgc tcaaggccgg tgcgctggag
     1081 atcggcgatg tgctggtcgt gacgaaggcc gatcgccccg aggcgaacga tctcgtccgc
     1141 gatctgcgtc gtctgcggac gctgaccttc gagccggttg tcgaggacgc ctctgtctgg
     1201 caggcgccga tcgtgaagac gagttcggtt cggctggagg gaatcgacga tctgctggtg
     1261 gccatcaccg cgcatcggga gtggctggaa gcgagcggtc ggctcgaatc gcgacggcgc
     1321 gagatagccg tggccgagat cgcgacgcgc gtccagacgc acctgcagcg aatcgcgcgt
     1381 caacagagta cccttgacgc cggatttggt gggatgatag atgaggttgt cgcccgccgg
     1441 ttgacgccgc aacgagcggc cgagcggctg gcgagtcgcc tctcgtcgaa atgacggacg
     1501 cgagaggatc gctgttcctg ggtaacggac cgcgctgaag tcgatgtcgt tggtcttgag
     1561 gaggacccca cgtcgtcggt cgccccgctg tgggcgagat tgccttgagg ttcgtttcct
     1621 atgaacagtg tctacgctgc gcttcccacg acgatcttcg agatcgtttc cgagacggcc
     1681 cggaagacgg gcgccgtcaa tctcggtcag ggcttccctg acgatcccgg gccggaggat
     1741 gtccgccgca aggctgccga ggcggtcgtc gatggatgga accagtatcc gccaatgctc
     1801 ggcctgcccg aactgcggca ggcggttgcc agtcactatg cccgtcatca ggggctcgat
     1861 ctcgatccgt cccgcgagat catggtgacg tcgggggcga ccgaggcgat tgccggggcg
     1921 ctctttgcac tgatcgagcc cggcgacgag gtgatcctct tcccgcccgt ctacgacgca
     1981 tacacgccgt tggtgcgccg tgcgggtggc attcccgtct tcgtgccgct gacgccgccg
     2041 gactgggaga tcacgcgcga ggcgctggaa gccgccgtaa caccgaaaac gcgcttcctg
     2101 ctcttcaaca atcccctcaa tcccgccgcg aaagtcttcc ccaggagtga actcgagatt
     2161 ctggcggcgt tctgcatcga gcacgacctg atcgcgatct gcgacgaggt ctgggagcac
     2221 gtca
//
LOCUS       DASTMD010000698         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2564693, whole genome shotgun sequence.
ACCESSION   DASTMD010000698 DASTMD010000000
VERSION     DASTMD010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2564693"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFQ54_10325"
     CDS             <1..197
                     /locus_tag="VFQ54_10325"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0165430.1"
                     /translation="RNAQMQKVPYMLVIGGREAADDSVSVRLRSGENLGAMKIQDFVE
                     LATPVIAARSLHCETRPATT"
     gene            443..1069
                     /locus_tag="VFQ54_10330"
     CDS             443..1069
                     /locus_tag="VFQ54_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU0165431.1"
                     /translation="MRKLVVNTFVSLDGVMQAPGGKGEDPEGGFDLEGWSVNYWDETM
                     NAAMGDFMGKPFDLILGRKTYEIFASFWPNASEAEGAGPLNAATKYVASTTQKNLEWG
                     PSELLGDDVVARIAELKRGDGPELQVHGSANLLQTLIAHDVIDEFRVLIFPVVLGKGK
                     RLFEAGVPPRGYKLVASQVAASGVILTTYHLAGEIELGTFGPDGEPIA"
     gene            complement(1154..>2065)
                     /locus_tag="VFQ54_10335"
     CDS             complement(1154..>2065)
                     /locus_tag="VFQ54_10335"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0165432.1"
                     /translation="FKYSNIGYSLLGLIIEAASGAPYREYVSANIVDKLGLKNTGPDL
                     DFDRLDDYAKGYTSRVHGPNRIEIEHIDTFAESSATGFYSTASELSAYFQAHLDGDDR
                     ILSEESKRRMRQIQWTVNAESGYGLGLISEKVNGRTYYGHSGGYPGHITMSKFDIERK
                     LSISVLTNSNDGPASVLATSILKLIDLALKKDAKTEPKDAAELAKFEGSFAALWGITD
                     IVNLGGRLYTLSSSMQDPAGAATEMEVVSENGLKPVGDEGFGGYGEVMRYTFREDGSV
                     EKVRGASGMRMVPKADFVLPEKLYRPA"
BASE COUNT          388 a          643 c          647 g          387 t
ORIGIN      
        1 ttcgcaacgc gcagatgcag aaggtcccct atatgctggt gatcggcggt cgtgaagcgg
       61 cagacgactc cgtctcggtt cggttgcgct ccggagagaa ccttggcgca atgaagatcc
      121 aggacttcgt cgagctggca acgccggtca ttgctgccag gtccctccat tgcgagacgc
      181 gcccggcaac cacctaagga ccgtcgatgc gtttggtggc actggagttg aggttggcag
      241 gacgggttct tgtgccccgc gcgcccgggg tggctgccgt cgtccgctcg gcgccggcgc
      301 cacaggcacc ggtgcacttc gccggaaatc ggcggaacac ggtgtcgaat tcggtctggc
      361 ccgttcgaca actacgtgag ctggacgaag gcagcccgca cgcgtgggcg aaccgtccgc
      421 cctgtatcga aggagatcaa ccatgcgcaa gctcgtcgtc aacacgttcg tttcgctcga
      481 cggggtcatg caggcaccgg gtggcaaggg cgaggatcct gaaggcggat tcgatctcga
      541 gggctggtca gtcaactact gggacgagac catgaacgcg gcgatgggcg acttcatggg
      601 aaagccattc gatctgattc ttggccgcaa gacgtacgag atcttcgcct cgttctggcc
      661 gaatgccagt gaggccgagg gtgccggtcc gctcaacgcc gccacgaaat acgtcgcctc
      721 caccacacag aagaatctcg aatggggacc gtccgagttg ttgggcgacg atgtcgtcgc
      781 gcggatcgca gaactgaagc gcggcgatgg tccagagctt caggtccatg gaagcgccaa
      841 tctcctgcag accctgatcg cccacgacgt gatcgacgag ttccgggtac tcatcttccc
      901 cgtcgtgctc ggcaagggaa agcgcctctt cgaggctggt gttccccctc ggggatacaa
      961 gctggtcgcc tcgcaagtcg cagcgagcgg ggtcatcctc accacgtacc acctcgcggg
     1021 tgagatcgaa ctcggcacct ttgggccgga tggcgagcca atcgcctgac gcgccgaagg
     1081 cttgcgggcg atcggaccgg gtacggctgg agacgaactc tagccgtacc cggctcccgg
     1141 gcatccagtg gagctacgct ggacggtaga gcttctctgg cagcacgaag tcagccttcg
     1201 gaaccatccg catgccggac gcaccgcgaa ccttctcgac gctgccatcc tcgcggaagg
     1261 tatagcgcat gacctcgccg tacccaccga agccctcgtc gccaaccggc ttgaggccgt
     1321 tctcggagac gacttccatc tcggtcgccg cgccggcagg atcctgcatc gacgaggaca
     1381 gcgtgtacag ccggccaccg aggttgacga tatccgtgat cccccagagc gccgcgaagc
     1441 ttccctcgaa cttcgcaagc tcggcggcat ccttcggctc ggtcttcgcg tccttcttca
     1501 acgcaagatc gatcagcttg aggatcgacg tagcaagcac ggacgccggc ccatcgttcg
     1561 agttcgtcag gacgctgatc gagagcttgc gctcgatatc gaacttcgac atcgtgatgt
     1621 gacccgggta gccaccgctg tgtccgtaat acgtgcgacc gttgaccttc tccgagatca
     1681 gacccagacc gtatcccgat tccgcgttca cggtccactg gatctgacgc atccgccgct
     1741 tcgattcctc ggagaggatg cgatcgtcgc catccagatg cgcctggaaa tacgccgaga
     1801 gttcagacgc ggtcgagtag aaaccggtcg ccgagctctc ggcgaaggtg tcgatgtgct
     1861 cgatctcgat gcggttcgga ccgtgaacgc ggctcgtgta ccccttggcg tagtcgtcga
     1921 gacgatcgaa gtcgaggtcc ggcccggtgt tcttgagccc gagcttgtcg acgatgttcg
     1981 cgcttacata ctcgcgatag ggagcgccgg atgccgcctc gataatgaga ccgagcagcg
     2041 agtagccgat gttggagtac ttgaa
//
LOCUS       DASTMD010000699         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2564740, whole genome shotgun sequence.
ACCESSION   DASTMD010000699 DASTMD010000000
VERSION     DASTMD010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2564740"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1340
                     /gene="kynU"
                     /locus_tag="VFQ54_10340"
     CDS             39..1340
                     /gene="kynU"
                     /locus_tag="VFQ54_10340"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEU0165433.1"
                     /translation="MPEFPPDRTTEAYARALDAADPLARFRDRFYIPDGVRYLDGNSL
                     GLLSRDAESAVHQAMEAWKTQGVSAWTAADPPWFTLAESLGARMAALVGATPESVIVT
                     GGTTINLHQMIATFYRPEGTRRKIVATSLDFPSDVYALQGQLLLHDGDPETDLVLVPS
                     RDGRTIATEDVLAAIGDDVALVLMPSVLYRSGQLLDIPAIARATHDHGALIGLDCAHS
                     VGAIPHEFDAWGIDFAIWCTYKYLNAGPGAVGALYVNPKHFGTRPLLSGWWGYQKDRQ
                     FEMAHAWEGASGAGAFQISTVPLLSTAALAGSLAITEEAGIDAIRTKSLAQTDYLIDL
                     VEASGLCGNPYDYRIGTPRPHAERGGHVAIEHDDAARISAALRQRGVIVDFRPPHVVR
                     IPPIALYTTYRDLWGTVRALREIIETGDHLDLDAGFAELVT"
     gene            complement(1350..1565)
                     /locus_tag="VFQ54_10345"
     CDS             complement(1350..1565)
                     /locus_tag="VFQ54_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165434.1"
                     /translation="MRETAHKSREDRATTITNLQKEVRDIQQQISDLSDAMDARPSGT
                     STDLQMTDLQRRLTEKQAELSRYQARI"
BASE COUNT          336 a          617 c          599 g          365 t
ORIGIN      
        1 agccagatat gatgcttgcg caacaggagg cttagctgat gcccgagttc ccaccggatc
       61 gcacgacaga ggcatacgcc agggcgctcg acgccgccga tcccctcgcc cggttccgcg
      121 atcgcttcta catcccggac ggcgtgcgct atctcgatgg caactcgctc ggcctccttt
      181 cgcgtgatgc cgaatccgcg gtgcatcagg cgatggaggc gtggaagaca cagggtgtca
      241 gtgcgtggac cgccgccgat cccccctggt tcacgctggc ggaatccctc ggcgcgcgga
      301 tggccgctct cgtcggcgcg acgccggagt cggtgatcgt caccggtggc accacgatca
      361 acctccacca gatgatcgcg accttctacc ggccagaggg aacacgccgc aagatcgtgg
      421 cgacgagtct cgatttcccc tccgatgtct acgcgctgca ggggcagctt ctcctccacg
      481 acggcgatcc ggagaccgat ctcgtcctcg tgccaagtcg cgacggcagg acaatcgcga
      541 cggaggatgt cctcgccgcg atcggcgacg acgtggcact cgtcctgatg ccgtcggtgc
      601 tctaccgctc cggtcagctg ctcgacatcc cggcgatcgc cagggccacg cacgatcacg
      661 gcgccttgat cggtctcgac tgcgcgcact cggtcggcgc gattccgcac gagttcgatg
      721 cctggggaat cgacttcgcg atctggtgca cctacaagta cctgaacgcg ggacccggcg
      781 cagtcggcgc gctttacgtc aatccgaaac acttcgggac aagacccctg ctctcgggct
      841 ggtggggata tcagaaagat cgtcaattcg agatggcgca tgcgtgggag ggggcatccg
      901 gcgccggggc cttccagatc agcaccgtgc cgttactctc gacggcggcc ctggctggat
      961 ccctggcaat cacggaggag gcgggaatcg acgcgatccg caccaaatcg ctcgcccaga
     1021 ccgattacct gatcgacctc gtcgaagcga gtggcctgtg cgggaatcca tacgattacc
     1081 ggatcggaac cccgcgtccg cacgcggaac gaggtggtca cgtcgccatc gaacacgacg
     1141 atgcggcgcg gatttcagcg gcgctgcggc aacgcggcgt gatcgtcgac tttcgaccgc
     1201 cgcatgtggt gcggatcccg ccgatcgcgc tctatacgac ctatcgcgat ctctggggaa
     1261 ccgtacgggc gctgcgcgaa atcatcgaga ccggcgatca cctcgatctc gacgccggtt
     1321 tcgctgaact cgtcacatag gggggagact cagatcctgg cctgatagcg agagagttcg
     1381 gcctgcttct cggtcaaccg gcgctgaaga tccgtcatct gaagatcggt gctggtgccg
     1441 gatggacgcg cgtccatcgc atcactcagg tcggaaatct gctgctggat gtcgcggact
     1501 tccttctgga gattggtgat cgtcgtcgcg cgatcttcac gcgacttgtg cgcggtttcc
     1561 cgcatttgat cgtaggttgg cactctccgc gaggagacct gcctctgtaa ccagttgcgc
     1621 aagttcgtca acatcgcggg gacctttccg gctattggtt ggggcaagaa gatgggaatc
     1681 cgtgctggtt cgagtctagc cggtttggca cgctcaggca ggcgatccga cccggatcgg
     1741 tccattcaat ctgccgtcac ccagttttcc agcgctagcg gtggcgcgat gcgcgcgaag
     1801 tgacgcgtgt tgttcgtcac cagcgtcgcc ccaagcgcga tcgcgtgagc ggcgatcatc
     1861 acatcgcgct ccccgaaaag ttctcctcga cgcctgaggt catcgtgtat atcggca
//
LOCUS       DASTMD010000700         4879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_688473, whole genome shotgun sequence.
ACCESSION   DASTMD010000700 DASTMD010000000
VERSION     DASTMD010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688473"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="VFQ54_10350"
     CDS             <1..1650
                     /locus_tag="VFQ54_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0165435.1"
                     /translation="ILFQQDAQSNVYAFNKETGEKVWTNTYNDAVPSGGPNGVGLGYG
                     MAVFTLGGSGIVIAADQKTGKEIWRTTIKGYRHEGITMAPLVYGSTVYVSTIPGTPEA
                     FYNGGQRGFIFALDAKTGGILWYFDTTTDNLWNNARINSGGGLWHVPAVDDQGKLYVG
                     IGNAAPYPGTKDFPAGTSRLGDNNYADSILKINPDTAAIEWFINVKPHDIFDLDNQLS
                     PVLGNVTINGVDTKVVFTSGKHGIVVAANQETGEELWRTPVGKHQNDDLQTIPNDGVT
                     KVEVFPGTLGGVETPFAFAEGVVFAPIVNFGTTYFATEIDAASLDFSKGTGQLVALDG
                     ATGKILWDVALPAPAYGAATVVNDVVFTAGLDAIVRGFKTSDGSAVWSWQAPAGVNAP
                     LAASGDFLYVPAGGPIYASADTQNPGTAPFPNLVALKLGGTATIAGGATPAATEATPA
                     ATPQAGGGAGTAFAVSTVDLAFDPKTLTIPANTDVKITVTNKGVLQHDFTIDELKVQS
                     KLLNGGDTDTVTINAAAGSYQYYCSVPGHKEAGMVGTLTVQ"
     gene            complement(1729..2352)
                     /locus_tag="VFQ54_10355"
     CDS             complement(1729..2352)
                     /locus_tag="VFQ54_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165436.1"
                     /translation="MLMRTIESNDHVMGRATRRRRFRRLIEQQFWLWGCGIRCPDGNK
                     LRACGFARTRSPEGQPAGTSRYMVILNSGQTLTLWGFGLTLSDPARGVLYLPRLRSTP
                     RYGPTGTDIDDRWTPADFAGFDLPRGDDRIRMRDLTADAFRWIAGYERRTLEVEGLAY
                     RTRCVRAWKDPIEPVRDLPWMWQSLAAESGRGDRASSLQPIALEASR"
     gene            2532..2843
                     /locus_tag="VFQ54_10360"
     CDS             2532..2843
                     /locus_tag="VFQ54_10360"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEU0165437.1"
                     /translation="MRIAFRMKVQAGKLDEYIAHHTAVWPELLADMRAAGYSNYSIFH
                     DGLDLFAFLECDDWDAANECLARSDANRRWQELMKNYLEGGVDPDQGPAQLMPEIFRM
                     E"
     gene            complement(2856..3860)
                     /locus_tag="VFQ54_10365"
     CDS             complement(2856..3860)
                     /locus_tag="VFQ54_10365"
                     /inference="COORDINATES: protein motif:HMM:NF036726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEU0165438.1"
                     /translation="MRQTSQASTIETLKQDEKIANWIGYLAEIGPSPEALPIPDERDL
                     RARFAYLAVPPDDLETLVALRPTIANDPDLLWILDRAVHSLTLNLDVVATVPGFPVLP
                     TSLGEIADFFYVYVYAMALPATLALHERHGVPSAVSEATMADIGRNMLVHRKRHGRHG
                     LAAPDWLMLHARGMIFQFGRLQFERAKLGTRTGDAIQAAGFPFRTGDPTLSIHIPDFM
                     GSMSVEACDESFAAARAFFPAHYPDEDVRTAVCNSWILDPQLRDYLRLDSNILRFQDR
                     FTEIRPAEWTNDGPLRFVFGPTDLPLEDYPQRSSVERAVVGHIAAGKNWHGGMGWLKF
                     "
     gene            4066..>4879
                     /locus_tag="VFQ54_10370"
     CDS             4066..>4879
                     /locus_tag="VFQ54_10370"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165439.1"
                     /translation="MATEQKAPANPAETDEFIWLEDIYGEAPLAWVAEQNQVTSRMLA
                     TPAFAQTQQQILDVLDSTERIPTVTRYGDFLYNFWKDADHPRGLWRRTSLEQYRTARP
                     EWETVLDIDALGKAEGTDWVFAGAQLLYPEYERALLHLSPDGGDAVTIREFDVSAKTF
                     VESGFVLPTAKTRITWSGHDSLLVATDFGPGTLTTSSYPRQVKRWGRGTPIESAELLF
                     EVPIEHTMVSVGHEHAEGYERDVLVDWVDFFHRNFYLVRGGEPVRIEIPDDAT"
BASE COUNT          909 a         1518 c         1607 g          845 t
ORIGIN      
        1 atcctcttcc agcaggacgc ccagagcaac gtctatgcct tcaacaagga gacgggcgag
       61 aaggtctgga ccaacacgta taacgatgct gttccgtccg gtggaccgaa cggtgtcggt
      121 ctcggttacg gcatggccgt cttcaccctc ggtggttccg gcatcgtgat cgccgccgac
      181 cagaagaccg gcaaggaaat ctggcggacg acgatcaagg gataccgcca cgagggcatc
      241 acgatggccc cgctggtcta cggcagcacg gtctacgtga gcacgatccc gggcacgccg
      301 gaggccttct acaacggcgg ccagcgcggc ttcatcttcg cgctcgatgc gaagaccggt
      361 ggcatcctct ggtacttcga tacgacgacg gacaacctct ggaacaacgc ccgcatcaac
      421 tcgggtggcg gtctctggca cgtcccggcg gtcgatgatc agggcaagct ctatgtcggt
      481 atcggcaacg cggcgccgta cccgggcacc aaggacttcc cggccggtac gagtcgcctc
      541 ggcgacaaca actacgccga ctcgatcctc aagatcaatc cggacacggc ggcgatcgag
      601 tggttcatca acgtcaagcc gcacgacatc ttcgacctcg acaaccagct ctcgccggtg
      661 ctcgggaatg taacgatcaa tggtgtcgac accaaggtcg tcttcacgtc cggcaagcac
      721 ggcatcgtcg ttgcggcgaa ccaggagacc ggcgaggagc tctggcggac gccggtcggc
      781 aagcaccaga acgacgacct gcagacgatc ccgaacgatg gcgtgacgaa ggtcgaggtc
      841 ttcccgggca ccctgggcgg cgtcgagacc ccgttcgcct tcgccgaggg ggtcgtcttc
      901 gcgccgatcg tcaacttcgg caccacctac ttcgcgacgg agatcgacgc ggcatccctc
      961 gacttctcga agggcaccgg tcagctcgtc gccctcgacg gcgcgacggg caagatcctc
     1021 tgggacgtcg cgttgccggc gcccgcgtac ggtgcggcga cggtcgtcaa tgacgtcgtc
     1081 ttcacggccg gtctcgatgc gatcgtgcgt ggcttcaaga ccagcgacgg gtccgcggtc
     1141 tggagctggc aggcgccggc gggcgtcaat gccccgctcg cggcgtcagg cgatttcctg
     1201 tacgtcccgg ctggcggacc gatctacgcc tcggccgata cccagaatcc ggggaccgcg
     1261 ccgttcccga acctcgttgc gttgaagctt ggcggcacgg cgacgatcgc gggcggcgcc
     1321 actccggccg caaccgaggc gactccggcg gcgacgccgc aggctggcgg gggcgccggt
     1381 acggcgttcg ccgtgagcac ggtcgacctc gcgttcgatc cgaagaccct gacgatcccg
     1441 gccaacaccg acgtcaagat cacggtgacg aacaagggcg tgcttcagca cgacttcacc
     1501 atcgatgagc tcaaggtcca gtccaagctc ctcaatggcg gtgacacgga cacggtgacg
     1561 atcaacgctg cggccggcag ctaccagtac tactgctcgg ttcctggtca caaggaagcc
     1621 ggcatggtcg ggacgctgac cgtccagtag acgtcgttcg acgttcagaa cctcgcgatg
     1681 gagaccccgg agccgaaagg ctccggggtc tctgcgagtt ccccgatcct accttgatgc
     1741 ttcgagcgcg atcggctgga gggatgatgc ccgatcgccc ctcccgcttt cggcggcgag
     1801 ggattgccac atccaaggaa gatcgcggac gggttcgatc ggatccttcc aggctcggac
     1861 gcagcgagtg cggtaggcga ggccctccac ctccagcgtc cgccgttcgt agccggcgat
     1921 ccatcgaaac gcatccgccg tcaggtcacg catgcggatt cgatcgtcgc cacgcgggag
     1981 atcgaagccg gcaaagtcgg cgggcgtcca gcggtcgtcg atgtcggttc cggttgggcc
     2041 gtatcgcggc gtcgatcgaa gccgcgggag atacaggact cctcgcgccg ggtcactgag
     2101 ggtcagccca aacccccaca gcgtaagcgt ctgaccggag ttcaggatca ccatgtaccg
     2161 gcttgttcct gccggttgtc cttccggcga gcgcgtcctg gcgaacccgc aggcgcggag
     2221 cttgttgccg tcggggcatc ggatgccgca tccccagagc cagaactgct gctcgatgag
     2281 ccggcggaag cgtcttcgtc tcgtcgcccg gcccatgacg tgatcattcg actcgattgt
     2341 gcgcatcaac accccgtcca tcgttgtgat cccatgttga gacgatatct caatatggga
     2401 atgctaggcg gtggcggatg ggtgcgtcaa catccgggcg cggcaggcga ttgacggagt
     2461 gcagcgatcg aggaaaatcg acagacacgc aacattgatg gattcgcgct gacggggagg
     2521 atcacgacgt ggtgagaatc gcgttccgaa tgaaggtaca ggccggcaag ctggatgaat
     2581 acatcgccca ccatacggca gtctggccgg agttattggc ggacatgcgt gcggcgggct
     2641 actcgaacta ctcgattttc catgacggac tcgacctctt cgcgttcctc gaatgcgatg
     2701 actgggatgc cgcgaacgag tgtctcgccc ggagcgacgc caaccgtcga tggcaggaac
     2761 tcatgaagaa ctatctcgag ggtggggtcg atcccgacca gggaccggcg cagctgatgc
     2821 cggagatctt ccgcatggaa tagagcggca tcggatcaga acttgagcca gcccatgccg
     2881 ccgtgccagt tcttgcccgc ggcgatgtga ccgacaaccg cgcgctcgac cgaggaccgc
     2941 tgcgggtagt cctcgagcgg caggtccgtg gggccgaaga cgaagcgaag cggtccgtcg
     3001 ttcgtccatt ccgccgggcg gatctcggtg aagcgatcct ggaaccggag gatgtttgaa
     3061 tccagacgca ggtagtcgcg aagctgcgga tcgagaatcc acgaattgca gacggccgtg
     3121 cggacgtcct catccggata gtgcgccgga aagaacgcac gggctgcggc gaacgactcg
     3181 tcgcaggcct cgaccgacat cgagcccatg aagtcgggga tgtggatcga gagcgtcgga
     3241 tcgcccgtcc ggaacgggaa ccccgcggcc tggatcgcgt ctcccgttct cgtcccgagc
     3301 ttcgcgcgct cgaattgaag gcggccgaac tgaaagatca tgccgcgcgc atgcagcatc
     3361 agccagtcgg gggcggcgag accgtgccgg ccatgccgct tgcgatggac cagcatgttg
     3421 cgcccgatgt cggccatcgt tgcctcggag acggcggatg ggacgccatg acgctcgtgg
     3481 agcgccagcg tcgccggaag cgccatcgca tacacgtaga cataaaagaa gtcagcgatc
     3541 tcgccgaggg atgtcggaag aacggggaag ccgggcacag tcgcgacgac gtcgagattc
     3601 agcgtcagcg aatggaccgc gcgatcgagg atccagagaa gatcgggatc gttcgcgatc
     3661 gtcggccgga gcgcgacaag cgtctcgaga tcgtcgggcg gaacggcgag gtaggcaaag
     3721 cgggcgcgga gatcgcgctc gtccggaatc gggagtgcct cgggggatgg cccgatctcc
     3781 gccaggtatc cgatccagtt ggcgatcttc tcgtcctgtt tgagtgtctc gatcgtgctc
     3841 gcctgcgagg tttgccgcat tgtcattcct gtcttcccct tcgtcgttcg aatcatacaa
     3901 ggaagaaggg tctcgtcgat ggtgaagcgc gagccgttct gacgggatcc ggacgcatcg
     3961 tggcgttccg gattgccgca tggacagtcg cgcacgcctg ggtcatgatg tggtgaatgt
     4021 ggcgggttca tcccgtgttc gatcgatgca tgacgaggtg aatccgtggc gacagaacag
     4081 aaggcgccgg ccaatccggc cgagactgac gagtttatct ggctcgagga tatctatggc
     4141 gaggcgccac ttgcgtgggt cgcggagcag aaccaggtta cgtcccggat gctggcgacc
     4201 cccgccttcg cgcagacgca gcaacagatt ctcgatgtcc tcgactcgac cgagcggatc
     4261 ccgaccgtga cccgctacgg cgattttctc tacaacttct ggaaggatgc cgatcacccg
     4321 cgcggcctct ggcgccggac gtcgctcgag cagtaccgga ccgcgcgtcc ggagtgggag
     4381 acggttctcg acatcgacgc actcggcaag gcggagggaa ccgactgggt cttcgcgggg
     4441 gcgcagcttc tctatcccga gtatgagcgg gcgctcctcc atctttcgcc tgatggcggc
     4501 gatgccgtga cgatccgcga gttcgacgtg agcgcgaaga ccttcgtcga gagcggtttc
     4561 gtgttgccaa cggcgaagac gcgcatcacg tggtccggtc acgactcgct cctggtggcg
     4621 accgatttcg gtccaggcac gctgacgaca tccagttatc cgcgccaggt caagcggtgg
     4681 ggcaggggaa cgccgatcga atcggcggag cttctgttcg aagtcccgat cgagcacacg
     4741 atggtctccg tcggccacga gcatgccgaa ggctacgagc gcgacgtgtt ggttgactgg
     4801 gtcgacttct tccatcgcaa cttctacctc gtgcgcggtg gcgagccggt ccggatcgag
     4861 attccggacg acgccacgg
//
LOCUS       DASTMD010000701         1504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_421149, whole genome shotgun sequence.
ACCESSION   DASTMD010000701 DASTMD010000000
VERSION     DASTMD010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421149"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFQ54_10375"
     CDS             <1..586
                     /locus_tag="VFQ54_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165440.1"
                     /translation="VGTPATTGVDDVIVTLLGFRNEADARDAFKLTSGTLVAGAILGE
                     SDQPLTSTPVKGLGDDATLYLGGKTYLGGDERAEGLLLVRSGALGIVASGEGRNASQL
                     SDMLRSFAAFIVDQKPSSTPVTVLGTGRATGGPFDLMPGPGDTSVLAGLVPVDDYDLL
                     VNDSPIKPAATPSGQAPSPGGLRIRVAGTGASVR"
     gene            complement(546..926)
                     /locus_tag="VFQ54_10380"
     CDS             complement(546..926)
                     /locus_tag="VFQ54_10380"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU0165441.1"
                     /translation="MTEHPSSEVDPGIMARMRAISLRLPEAAEAIVFGSPTFQVRAKN
                     FAMLRQEESRTSVWCKAAPGVQEQYLDRDPVRYYRPPYVGPKGWIGAWLDEEIDPDWD
                     DIAEMIETSYRLIAPKRLSRQLES"
     gene            complement(977..>1504)
                     /locus_tag="VFQ54_10385"
     CDS             complement(977..>1504)
                     /locus_tag="VFQ54_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEU0165442.1"
                     /translation="IVGDSVADPKSFDGLRQMISGGQLLSMGTDGGALSLGKMDELVD
                     AVVPGKPDLLLMSKRSRRKLKDLRRSSGAVLEMSVNQFGERIEVYDGIPVVVDDFMPD
                     NETQGTGTALSSIYALKFGQSTGLMGLEHGGITVEPVGSLETKDATRTRIKWYAGLAL
                     FNSLGVARLSGITAA"
BASE COUNT          241 a          503 c          488 g          272 t
ORIGIN      
        1 cgtggggaca cccgcgacca ctggcgtcga tgacgtcatc gtcaccctgc tgggattcag
       61 gaacgaggcg gacgcgcggg atgcgttcaa gctgacctcg gggacactcg tcgccggcgc
      121 gatcctcggc gagagcgatc agccgctgac gagcacaccg gtcaagggac tcggtgacga
      181 cgcgacgctt tatctcggtg ggaagaccta tctcggtgga gacgagcgcg ccgaaggtct
      241 cctgctggtg cgttcgggcg cgctcggcat cgttgcaagc ggtgagggga ggaacgcctc
      301 ccaactgagc gatatgctgc ggtcgtttgc cgcgttcatc gtcgatcaga agccgtcgtc
      361 caccccggtg acggtgctcg gaaccgggcg ggcgaccgga ggaccgttcg atctcatgcc
      421 gggtccgggc gacaccagcg tgttggccgg gctcgtcccg gtcgatgact acgacctgct
      481 cgtcaatgac tcgccgatca agcccgcggc cacgccgagc ggccaggcgc catcgcccgg
      541 tggtctcagg attcgagttg ccgggacagg cgcttcggtg cgatgaggcg gtagctcgtc
      601 tcgatcatct cggcgatatc gtcccagtcg ggatcgatct cctcatccag ccaggcgccg
      661 atccatccct tcgggccgac gtagggcgga cggtagtagc ggaccgggtc gcgatcgaga
      721 tactgctcct gcacgccggg cgccgccttg caccagacag acgtccggga ctcttcctgg
      781 cggagcatcg cgaagttctt cgcccgcacc tgaaacgtgg gcgagccgaa cacgatcgct
      841 tccgccgcct cggggagacg caggctgatg gcgcgcatcc gcgccatgat gccgggatcg
      901 acctcggacg atgggtgttc ggtcatctgc gtctcctctc gaatccgtcc cggcgattcg
      961 tccggctgcg atctcatcac gcggcggtga tgccgctcag ccgggcgacg ccgagcgaat
     1021 tgaacagtgc gagtcctgcg taccacttga tgcgcgtgcg ggtcgcatcc ttcgtttcaa
     1081 gcgatcccac cggctcgacg gtgatgccgc cgtgttcgag gcccatcagc ccggtgctct
     1141 gcccgaactt cagcgcgtag atcgagctga gcgcggtgcc ggttccttgc gtctcattat
     1201 ccggcatgaa atcgtccacc acgaccggga tgccgtcgta gacctcgatc cgctcgccga
     1261 actgattcac cgacatctcg agcaccgcgc cgctgctgcg tcgcaggtcc ttcagcttgc
     1321 gtcgcgagcg cttgctcatc agcaacagat ccggcttgcc gggaacgacc gcgtcgacca
     1381 gttcatccat cttcccgagg ctgagggcgc cgccatccgt gcccatgctc aacagctgtc
     1441 cgccggagat catctggcgg aggccgtcga acgacttcgg gtcggccacc gagtctccga
     1501 cgat
//
LOCUS       DASTMD010000702         3997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1852079, whole genome shotgun sequence.
ACCESSION   DASTMD010000702 DASTMD010000000
VERSION     DASTMD010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852079"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFQ54_10390"
     CDS             complement(<1..735)
                     /locus_tag="VFQ54_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165443.1"
                     /translation="MIACVRILNFALRLEILEKPELDGDPLVLSPAQGERRKVIDCTP
                     EATALYIRPGMSIRDVIAMSPAAHVVDTNPAREARVSQKILHDLEALSPFVEADEREA
                     GCWYIDLVGLDRLLGPLPDIARRLLQTISPLLRPRVGIASGKFPARVAAGSSGPGAFQ
                     IVVPADTTAFLATASVTALPIDPATIQLLRQLGLRTLGELAVLSPSAVAARLGPEGRA
                     AWELARGNDDSVVKAARHREIIREHLA"
     gene            complement(999..2165)
                     /locus_tag="VFQ54_10395"
     CDS             complement(999..2165)
                     /locus_tag="VFQ54_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEU0165444.1"
                     /translation="MRADLETEIVPFTIDIPDEDLVDLRQRLTRARFAQGLSGVGWSY
                     GIPEEYVRELAAYWRDDYDWRAWEAKLNVYPQFTATIDGQKIHFLHLRSAELDATPLI
                     LLHGWPGSIVEFLDVIGPLADPVAHGGEAKDAFHLVIPSLPGFGFSGPTHETGWTSTR
                     MARALAGLMGRLGYERYGAQGGDFGAFVAPDLGRVDPEHVIGVHVNAATVGFIPWGEI
                     DPVEFETFTDLERERLARMQNFLSDGRGYFQIQATRPQTVAYGLADSPVGQLAWIAEK
                     FHEWSFNPEHVPGGPIDRDRLLTDVMLYWLTNTAASSARMYYEGMHSHERPQPSPVPT
                     GVAAFAEDIAIRRYGEQGNNIVHWSDFDRGGHFAAMEAPDLLVGDVRAFFRSLR"
     gene            2318..3277
                     /locus_tag="VFQ54_10400"
     CDS             2318..3277
                     /locus_tag="VFQ54_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEU0165445.1"
                     /translation="MIETSARLLRLLSLLQARRDWPGSELADRLAVSSRTIRNDIERL
                     RQLGYPVDATRGATGGYRLGTGATMPPLLLDDEEAVAVVVGLIRVTNGGIADVEESSL
                     RALTKLEQILPARLRRRVDAMRTFAVQVPPDSTGPTVSAETLSLLANACRDREKLRIA
                     YARYDGETSRRIVEPHRVVNWDRRWYLVAWDDDRTDWRTFRVDRIARLDPTGLRFGDR
                     DLPEGNITTYVARNVARAGWRYTARFRVQAAAEVVLDRINPAVGIVEAVDDETCILMS
                     GADSLDTVAVYIGMLGFDFTVEDPPELIAYLRATAERYQRAIS"
     gene            3328..3762
                     /locus_tag="VFQ54_10405"
     CDS             3328..3762
                     /locus_tag="VFQ54_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165446.1"
                     /translation="MSRAARSLRAVHALIALVELVCLAYLWFCAVTNRRDRKLATAIA
                     ILGAEGVGLIVGGGNCPLGPFQRHLGDSTPLFELVLPKRAAKAAVPILTGVAVLGVAI
                     VVVRSRVRPHRASQARFPSVTGMLLPDWSLGRRSGRRRRVHQ"
BASE COUNT          738 a         1298 c         1221 g          740 t
ORIGIN      
        1 tgccaggtgc tcgcgaatga tttcgcgatg acgtgctgct ttcaccaccg aatcatcgtt
       61 cccccgggcc agttcccagg cggcacgtcc ctccggaccc agtcgtgcgg ccaccgccga
      121 gggcgagagc accgccagct ctcccagcgt cctgagtccc agctgcctga gcaactggat
      181 cgtcgccgga tcgatcggca acgccgtcac cgatgccgtt gccaggaatg ccgtggtatc
      241 cgcgggcacg acgatctgaa aggcgccagg cccactggac ccggccgcca cccgggcggg
      301 aaacttgcca ctggcgatcc ccacccgggg acgcagcagc ggggagatcg tctgcagcaa
      361 acgccgcgcg atatccggca gcggccccag caaccgatcg aggccgacaa ggtcgatgta
      421 ccagcaacca gcctcacgct catccgcttc cacgaacggg ctgagtgcct cgagatcgtg
      481 aaggatcttc tggctcacac gcgcttcgcg agcggggttc gtgtccacga catgcgcggc
      541 ggggctcatc gcgatcacat cccggatcga catccccggt cgaatgtaga gcgcggtcgc
      601 ctccggcgtg caatcgatga ccttgcgccg ttctccctgg gccgggctca gaaccagtgg
      661 atcgccatcg agttcgggct tttcgagaat ctccagtcgt aacgcgaagt tgagtatccg
      721 cacacacgca atcatctttg ttccatcctc cacgatcgcg acgtgttcct gacaaccaga
      781 gtagaacata tgttctgtta ccgcaagaca ttaacggagt tgacttaaag gcactcaact
      841 gcacgtgcac acaaaatatg tctgcgggga cgatcgacct gccatcagga gcgaagaccc
      901 ctcatcgatc tcccttgcca gacgcaccag gcatgacgaa gcccgggctg cggcaaacac
      961 gcggtccggg cttcactatc acaggcagat ggagatcgtc agcgcagact ccgaaagaac
     1021 gcccggacat cgccaacgag cagatcgggt gcctccatcg ccgcgaaatg accgccgcgg
     1081 tcgaaatcgc tccagtgcac gatgttgttc ccctgctctc catatcgccg gattgcgatg
     1141 tcctcggcaa aggcagcgac gcccgtcggc accggcgatg gctgcggccg ttcgtggctg
     1201 tgcattccct cgtagtacat ccgggccgac gaggccgcgg tgttcgtcag ccagtagagc
     1261 atcacgtcgg tcaggaggcg gtcgcgatcg atcgggccac cgggaacgtg ctccgggttg
     1321 aagctccact cgtggaactt ctcggcgatc cacgccagct gcccgaccgg tgaatccgcc
     1381 agcccgtagg caaccgtttg cgggcgcgtc gcctgaatct ggaagtaccc acggccatcg
     1441 gagagaaagt tctgcatccg cgcgagccgc tccctctcga ggtccgtgaa ggtctcgaac
     1501 tcaaccggat cgatctcgcc ccacgggatg aaccccaccg tcgccgcgtt gacatgaacg
     1561 ccgatcacgt gctccggatc gacgcgtccg agatccggag cgacgaaggc gccgaaatct
     1621 ccaccctgtg cgccgtagcg ttcatacccg agcctgccca tcaatccggc gagtgcgcgc
     1681 gccatccgtg tcgaggtcca acccgtctcg tgcgtcggac cggagaaacc gaagcccggt
     1741 agcgacggaa tcacgaggtg gaaggcatcc ttcgcctcgc caccatgggc gacgggatcg
     1801 gcgagcggtc cgatcacatc gagaaactcg acgatcgaac ccggccaacc gtggaggaga
     1861 atcagcggcg tcgcgtcgag ttcggcggag cgaaggtgca ggaagtggat cttctggccg
     1921 tcgatcgtcg cggtgaactg cggatagacg ttcagcttcg cctcccacgc ccgccagtca
     1981 tagtcgtcgc gccagtaggc ggccagctcg cggacgtatt cctccggaat tccgtaagac
     2041 caaccgacac ctgacagtcc ctgcgcgaag cgtgcccgcg tcagccgctg cctgagatcg
     2101 acgagatctt cgtccgggat atcgatcgtg aaaggaacga tttcagtctc gagatcggca
     2161 cgcatcctgg tgctccttcg tcgtttgagg tcatccggtg acgtccggcc caacggccat
     2221 agagatagaa tagaagtcaa tcaggcacgc ttcgttcctg attcgaatcc atgattcatt
     2281 caacgattcg agcgtcggga ttgatcggat tgaccatgtg atcgaaacat cggcgcgact
     2341 gttgcgcttg ctctcactcc tgcaagctcg ccgcgattgg ccgggatccg agttggcgga
     2401 tcgactggcg gtcagttcgc gcaccatccg caacgacatc gagcggctgc gtcagctcgg
     2461 ctacccggtc gacgcgaccc gcggcgccac cggtggctac cgcctcggca ccggcgcgac
     2521 gatgccgccg cttcttctgg acgatgagga agcagttgcc gtcgtcgtcg gtctgatccg
     2581 ggtcaccaac ggtggaatcg cggacgtgga ggagtcctcg ctccgcgcac tgacgaagct
     2641 cgagcaaatc ctgccggccc ggctgcgtcg ccgcgtcgac gcaatgcgga ctttcgccgt
     2701 gcaggtacct ccggattcga ccggaccgac ggtctctgcc gagacgctct ccctgctcgc
     2761 gaatgcctgc cgcgatcgcg agaagctgcg catcgcctac gcgcgctacg atggcgagac
     2821 aagccgccgc atcgtcgaac cacatcgcgt cgtgaattgg gaccgtcgct ggtacctggt
     2881 cgcctgggac gacgatcgca ctgactggcg gacgtttcgc gtcgatcgca ttgcgcggct
     2941 cgatccgaca ggtttgcgtt ttggggatcg cgatctgccg gagggcaata tcacgaccta
     3001 cgtcgcgcga aatgtcgcgc gcgccggctg gagatacacc gcgcgctttc gcgtgcaggc
     3061 agcggcggag gtggtgctgg accggatcaa tcccgccgtc gggatcgttg aggcagtcga
     3121 cgacgagacc tgcatcctga tgtctggtgc cgatagtctc gacaccgtgg ctgtctacat
     3181 tggcatgctc ggattcgatt tcacggtgga ggatcctccc gaactgatcg cgtacctccg
     3241 cgcgacagcc gaacgctacc agcgggcgat ctcatgatgg gatcatctcg tgatactcgc
     3301 gcggtcgtca cgtcaggagg aggatgaatg tcccgtgctg cgcgaagcct tcgagccgtt
     3361 cacgcgctca tcgcgctggt tgaactcgtg tgtctcgcct acctgtggtt ttgcgccgtc
     3421 acgaaccggc gcgatcgcaa gctggccacg gcgatcgcga ttctcggcgc ggaaggggtt
     3481 gggctcatcg tcgggggcgg aaattgtcca ctcggcccct tccagcggca tctgggcgat
     3541 tcgaccccac tcttcgaact ggtcctcccg aagcgggcgg caaaggccgc ggttcccata
     3601 ctgaccgggg tcgcggtgct cggcgtcgcg atcgtcgtgg tgcgatctcg agttcggccg
     3661 catcgagcca gccaggcacg attcccgtcg gtcacgggga tgttgcttcc tgactggtcg
     3721 ctcggtcgac ggtctggtcg aagaagacgc gttcaccaat gagggggttg ccgggacacg
     3781 gcgtcggccg gcgcgaagtg ggacattcct tgctcgccca gtattcgatc gtcggtcgag
     3841 cgtttcggat ttcgccccag ttggcaccgg tgaagcagat ggatgtgagt agtcgttccc
     3901 atttggttat tgacattaac ccaataggga cataccatca ccccatgaac acgatatccc
     3961 ttccccagac gatctccttt cgccccctcc tgaaacg
//
LOCUS       DASTMD010000703         1859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1046541, whole genome shotgun sequence.
ACCESSION   DASTMD010000703 DASTMD010000000
VERSION     DASTMD010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046541"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..1613
                     /locus_tag="VFQ54_10410"
     CDS             300..1613
                     /locus_tag="VFQ54_10410"
                     /inference="COORDINATES: protein motif:HMM:NF011657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEU0165447.1"
                     /translation="MVESTGGPKVTLVGAGSTVFAKNLIGDILSYPELSGSTIALFDI
                     DDERLKTSEIVAKKLASSVNAPATIVATTDRKEALRDADYVIIMIQVGGYKPSTVIDF
                     EIPKKYGLRQTIADTLGIGGIFRALRTIPVLIDVAKDMEEVCPNALFLNYVNPMAMNC
                     WAISRASDISTVGLCHSVQGTAGELARDIDVPVEEINYIAAGINHYAFYLKFERDGVD
                     LYPEINKVIAEGRVPDHNRVRYEMLKRTGYFVTESSEHFSEYGPWFIKRDRPDLIEQF
                     NIPLDEYIRRCEEQLARWGRMRAEYEGDAPIEHKRSHEYASRIILGHQTGNPEVIYGN
                     VPNHGLIDNLPDGCCVEVPILVDRNGLQPTTIGSLPPQLAAMMQTNINVQALTVEAAI
                     TKKREHIYHAAMLDPHTAAELDLDQIWHLVDDLIEAHGDWLPTYS"
BASE COUNT          396 a          599 c          542 g          322 t
ORIGIN      
        1 atgagatcga agctcttccc atcaagcgac gccaattcct gcttcacctt acgtcccctt
       61 agttcacgct attccgacga gatatgcccg ggcgatacct caactctgcc gcaattgtag
      121 gacgacccgg gatccggttc cggtgattcg catcttcttt aatgattcga gttcggatcg
      181 ccacaccggg aggggtttca attcgctctt gccgcaatcg cgcttgtatg cggagaatat
      241 ccccaacgtg cgacgcagcg acgcgccgcg atcagcgaac gaaggagcga caccgtttca
      301 tggtcgaatc aacaggtggc ccgaaggtca cgctcgtcgg agcaggcagc accgtcttcg
      361 cgaagaatct gatcggcgac atcctcagct atcccgaact gagtggaagc acgatcgcgc
      421 tcttcgatat cgacgacgag cgtctgaaga cgtcggagat cgtggcgaag aaactcgcct
      481 cctccgtcaa tgccccggcg acgatcgtcg ccacgaccga tcgcaaggaa gcactgcgcg
      541 acgcggatta tgtcatcatc atgatccagg tcggtggcta caagcccagc accgtgatcg
      601 acttcgagat tccgaagaag tacgggcttc gccagacaat cgccgacacc ctcgggatcg
      661 gaggcatctt ccgggcactg cgcacgatcc cggtcctgat cgatgtcgcc aaggacatgg
      721 aggaagtctg cccgaacgcg ctcttcctga attacgtcaa tccaatggcg atgaattgct
      781 gggcgatctc gcgtgccagc gacatcagca cggttggcct ctgccacagt gtccagggca
      841 cggccggcga gctcgctcga gacatcgacg tcccggtcga ggagatcaac tacatcgcgg
      901 cgggcatcaa tcactacgcc ttctatctca agttcgagcg ggatggtgtc gatctctatc
      961 cggaaatcaa caaggtgatc gccgaagggc gggtcccgga ccacaaccgc gttcgctacg
     1021 aaatgctcaa gcgcaccggg tatttcgtga cggagtcgag cgagcacttc agcgagtacg
     1081 ggccgtggtt catcaagcgc gatcgtcccg acctgatcga gcagttcaac atcccgctgg
     1141 acgaatacat ccgccgctgc gaggaacagc tcgcccgctg gggacgcatg cgcgccgagt
     1201 acgagggcga tgcgccgatc gagcacaagc gatcgcatga atacgcctcg cggatcatcc
     1261 tcggtcacca gaccgggaat cccgaggtca tctacggcaa cgtcccgaac cacggtctga
     1321 tcgacaacct gccggacggc tgctgcgtcg aggtgccgat cctcgtcgac aggaacggcc
     1381 tccagccgac gacgatcgga tcgcttccgc cgcagctcgc cgcgatgatg cagacgaaca
     1441 tcaacgtcca ggcgctgacg gtcgaggcgg cgatcacgaa aaagcgcgag catatctacc
     1501 atgcggccat gctcgatccc catacggcgg cagagctcga tctcgaccag atctggcacc
     1561 tcgtcgacga tctgatcgaa gcccatggcg attggctgcc aacctattcg taagctggcc
     1621 caccgcaaag ggggggacag agaagtcccc ctgtactcac ggacgccgga agcaacgcgg
     1681 ttgggcgggc ggcgggacac ggcaacgccc tcgcataccc attcgccgtt tcgcgcctgt
     1741 cgagaaatcc ccggggccgt aggggcgagc ctcgtgctcg ccctccgccg caggcggacg
     1801 ccaacgcgaa caaccggtgc acgacgttcc ccggtcgctc gccgatcacg cctagggga
//
LOCUS       DASTMD010000704         2743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_688882, whole genome shotgun sequence.
ACCESSION   DASTMD010000704 DASTMD010000000
VERSION     DASTMD010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688882"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..>2743
                     /locus_tag="VFQ54_10415"
     CDS             437..>2743
                     /locus_tag="VFQ54_10415"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEU0165448.1"
                     /translation="MRARLLGATELRVGERILTSHDWTRRQARSVLLLLLGTPGHRLP
                     RDQVLESIWPDQEYESSLNSLYKALHALRRTLEPGLTSGRGSAYIETSSDVIALKPHE
                     GMWVDVDLFVTPIAQASANDKAGRRTRLRAAADLYRGDFIADDPYADWPVARREALRS
                     SWERTVLSLAHSDIEHGEPLASLGHLEALIGSDPTFEEGHRAIIRACLAAGQRAAALQ
                     QFERCRLALERELAVDPDAETKALIASARQLPVSGGEVGGTLPAFHNVPALPTRTVGR
                     RQEIAAAGDLLAQEDIRLVSIIGTGGVGKTRLAIEIARDAAGGFADGVAFVPLAAIRD
                     AGLLFAMIGNALQLRESSERPIAEIVTNFLLHRRFLLVLDNLEHLPNVANPIAELLAA
                     APGLTILATSRAPLRLRAEHLFPLGVLAIPDLAIATDRLDDIDSVALFLQCMRAWRPD
                     SPDADDDLETIAELCVRLEGLPLAIELAASRTWDTTPAAILAQLEDRFGALRDGPRDL
                     PDRHQTLRQTIAWSYELLSSAERELFRNLSIFSGGAELDALTAICGPDAVAVADLLVE
                     KSLAGWELGGGQRRLLMLESIREYAAELPATSEERNELAARHADFFADLVRRAEPALQ
                     GPDQLRWLDRLDRDLGNVRAALDWAIASDRAALALQMMSAAGHFWFFRGSNREGLSWL
                     QRGFSGIREDEIDTDGAARAARWGSLLAANLGEQAEVKRFNEIVIAAAAHSKDRQIIG
                     SALMLRAALARNSGEYAEARTLTLEALEN"
BASE COUNT          431 a          960 c          866 g          486 t
ORIGIN      
        1 gccagctctc cgccgtggtg gtggtgtccc agtcgaagag gggatgtaag attcgactcc
       61 cttcgtgtct cgttgaagag atgtgcgggc tgacaggcgt cttcccgcgc gtgactccac
      121 gagttccccg gacgagcccc gcgcatctga catgtagtta cgaacggttg agaggaacct
      181 tcgtgtacta catgctgaac gacttcggtc gttcgtcggc tggttcaaag tcgctgtttc
      241 cgccggcgcg atcgcccgga tgggcctccg cgaagggatc atgccgcgcg ggatcggggc
      301 atcccctctt gctcccgttc ccaggttgac gcattattga accaacgcct ccttcccgtc
      361 cggatcctca ccctcgtcac atctgggccg cgccatggat cgacaccacg acgccgggac
      421 acgacggatt ccgccattgc gtgcccgtct gctgggcgcg acggaactcc gcgtcggcga
      481 gcggattctg acctcccacg actggacgcg tcgccaggcg cggtccgttc tcctgctgct
      541 gctcggaaca ccggggcacc gcctgccgcg cgatcaggtg ctcgaaagca tctggccgga
      601 tcaggaatac gagtcgagcc tcaactccct ctacaaggcg cttcatgccc tgcgccggac
      661 cctcgagcca ggtctgacct ccgggcgtgg ctccgcctat atcgagacga gcagtgatgt
      721 catcgccctc aagccgcacg agggaatgtg ggtcgatgtc gatctcttcg tgacccccat
      781 tgcccaggcg tccgccaacg acaaggctgg gcgacgaacc cggcttcgcg cggcggccga
      841 tctgtaccgg ggcgatttca tcgccgacga cccatatgcc gattggccgg ttgcgcggcg
      901 ggaggcactc cgaagctcct gggaacgcac ggttctctcg ctcgcccact ccgatatcga
      961 gcacggtgag ccgctggcct cgctcggcca tctcgaagcg ctgatcggat ccgacccaac
     1021 gttcgaggaa ggtcaccgcg cgatcatccg cgcctgtctc gccgccgggc aacgggcggc
     1081 ggcgctgcaa caattcgaac gctgtcgcct cgccctggaa cgagaactcg cggtcgaccc
     1141 cgacgccgag acgaaggccc tgatcgccag cgctcgccag ttgccggtat ccggcggaga
     1201 agtggggggc acccttccgg cattccacaa cgtacccgcc ctgccgacgc gaacggtcgg
     1261 tcgccgccag gagatcgccg ccgccgggga cctgctcgcg caggaagaca tccggctcgt
     1321 ctcgatcatc gggaccggtg gcgtcggcaa aacccggctc gcaatcgaga tcgcccggga
     1381 tgcggccggg ggattcgcgg atggcgtcgc cttcgtcccg ctggcggcga ttcgcgatgc
     1441 cgggttgctc ttcgcgatga tcgggaacgc gctccagctc cgcgaatcgt cggagcgacc
     1501 gatcgccgag atcgtcacga acttcctcct ccaccgccgg tttctcctcg tcctcgacaa
     1561 tctcgaacac ctgcccaacg tcgcgaatcc gatcgccgag cttctcgccg ccgcgcccgg
     1621 gttgacgatc ctcgcgacaa gtcgcgcacc cctgcgcttg cgggcggaac acctctttcc
     1681 gctcggcgtc ctcgccatcc ccgatctcgc gatcgcaacg gatcgcctgg acgatatcga
     1741 ttccgtcgcc ctcttccttc agtgcatgcg ggcgtggcgt cccgactcgc ccgacgcgga
     1801 cgacgatctc gagacgatcg ccgaactctg cgttcgtctc gaagggctac ccctcgcgat
     1861 cgagctcgcc gcctcccgga cctgggacac cacgccggcg gcgatcctcg cccaactcga
     1921 agaccgcttc ggggccctgc gcgacggccc gcgcgatctg ccggatcgcc atcagacgct
     1981 tcgccagacg atcgcctgga gttacgagct tctttcctcg gccgaacgcg agctgttccg
     2041 caacctcagc atcttctccg gcggcgccga gctcgatgcc ctgaccgcga tctgtggacc
     2101 ggatgcggtc gccgtcgccg atttactggt ggagaagagt ctcgccggct gggagttggg
     2161 tggcggtcag cgccgcctgc tgatgctgga gtcgattcgc gaatacgcgg cggagcttcc
     2221 cgccacgagc gaggaacgca acgagctcgc agcgcgtcat gcggacttct tcgcggatct
     2281 cgtccgacga gcggaaccgg cgcttcaggg tcccgaccag ctccggtggc tcgatcgcct
     2341 cgatcgcgat ctcggaaacg tccgtgccgc gctcgactgg gcgatagcgt ccgatcgcgc
     2401 cgcgctcgcc ctccagatga tgagtgccgc cgggcacttc tggttcttcc gggggagcaa
     2461 tcgcgagggt ctgtcgtggt tgcagcgcgg attttccggg atccgtgagg acgagatcga
     2521 caccgacggc gctgcccgtg cggcccgttg gggttctctc ctcgcggcga acctgggaga
     2581 gcaggcggag gtgaagcgat tcaacgagat cgtgatcgcc gcggcggccc actcgaagga
     2641 tcgccagatc atcggatccg cgctcatgct gcgcgccgcc ctggctcgca acagtggcga
     2701 gtacgcggaa gctcgaacgc tcacgctgga ggcgctcgag aat
//
LOCUS       DASTMD010000705         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_688937, whole genome shotgun sequence.
ACCESSION   DASTMD010000705 DASTMD010000000
VERSION     DASTMD010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688937"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..1132
                     /locus_tag="VFQ54_10420"
     CDS             371..1132
                     /locus_tag="VFQ54_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165449.1"
                     /translation="MREHKLHPANPVPNVNVPVSSAIVGESALASERAERVRQEIAIR
                     ALPETRRLMLEILKREGTADTEHLARKTEITTSGARQHLVALESDGLIVHDVERAGPG
                     RPRHAYRLSVAGDALFPRHYGALTNELLAYVEDEDPAMLERIFARRGQRRLDQALART
                     TDQPFREKVRIVAQILDDDGYLADFAEAEDGSFIITEHNCAVLSVAQRYRHACSSELD
                     FLQRVLPESEVTRIAHRINGGHACAYRIVPQVHAD"
     gene            1262..>1586
                     /locus_tag="VFQ54_10425"
     CDS             1262..>1586
                     /locus_tag="VFQ54_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165450.1"
                     /translation="MSDDHSPGDARKPYSDRGGPAGRHAETHDVYREQLTNPKGPTPV
                     DETFAGDLAPQTPDTIRQEQVDDTVVASSDKRVRDELPELDNAQLSRLSILAPDTPLE
                     QGAVYL"
BASE COUNT          313 a          517 c          498 g          258 t
ORIGIN      
        1 gccgccgagc aggcgcgcga aggcgggtct ccggagcgga gagtgcgcga acgtggcgcc
       61 gacacgcgac gtgagtcgtg cgttcatcat ctcgtcgttt cgtgtggatg agcggctgtc
      121 aggacatgac ggcgaagcac gcgaaagggg caaacggttg cccgctccca tcctgcgtga
      181 tgacagcaac ccgggcctgg cggaagacca ggaacgaccg gaacacgaag cgtttgtgga
      241 tgcacggtag cagaggacga tgggtaaagc aagtaatatc tgtgtttact gggataaccc
      301 gatcgtgcag gatatactcc ctgcaaagac gccgtcgcga agacggcgtg cctctcgtga
      361 gagatgactc atgcgcgaac acaagctcca tcctgccaat cccgtcccga atgtcaacgt
      421 acctgtgtcg tcggccattg taggggaaag tgccctcgca agtgaacggg cggaacgggt
      481 gcgccaggag atcgcgattc gcgcgctccc ggaaacgcgc cgtctgatgc tcgagatcct
      541 gaagcgcgaa ggtaccgccg ataccgagca tctcgcccgg aagacggaga tcaccaccag
      601 cggcgcccgc caacacctcg tcgcgctcga gtccgatggt ctgatcgtcc acgatgtcga
      661 acgggccgga cccggtcgcc cccgtcatgc ctatcgtctc agcgtcgccg gagacgcgct
      721 ctttccgcgt cactacggtg cgctgacgaa cgaattgctc gcctacgtcg aggacgagga
      781 tcccgccatg ctcgagcgga tcttcgcccg gcgcggtcag cgccggctcg atcaggcgct
      841 cgcgcggacg accgatcagc cgtttcggga gaaggtgcgg atcgtcgccc agatcctcga
      901 cgacgacggg tatcttgccg acttcgccga agccgaggat ggctcgttca tcatcaccga
      961 gcacaactgt gccgtgctct cggttgccca gcgctaccgc cacgcgtgca gcagcgaact
     1021 cgacttcctg caacgcgtcc tgccggagtc cgaggtgaca cggatcgccc atcggatcaa
     1081 cggtggtcac gcctgcgcct accggatcgt cccccaggta cacgccgatt gacggcggcc
     1141 gcggtgcgat ggccgcgaaa atgaaacgcg agaacgcatt cgtgaccagg tagccaccgc
     1201 caaccgttgc gcgttggcat cgcccctgcg tgatcgaagg tgatgaaagg aggtgtctcc
     1261 gatgtccgat gatcacagcc cgggcgatgc ccgcaagccc tattccgatc gcggtggacc
     1321 ggccggtcgt catgccgaaa ctcacgacgt ctaccgcgag cagttgacga atccgaaggg
     1381 accgacgccc gtcgacgaaa ccttcgccgg ggatctcgct ccgcagacgc cggatacgat
     1441 ccgtcaggag caggtcgatg acacggtggt tgcgtcgtcc gacaagcggg ttcgggacga
     1501 actccccgaa ctcgacaacg cgcaactctc gcggctctcg atcctcgcgc ccgacacgcc
     1561 cctcgaacaa ggcgccgtct atctcg
//
LOCUS       DASTMD010000706         3891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2386389, whole genome shotgun sequence.
ACCESSION   DASTMD010000706 DASTMD010000000
VERSION     DASTMD010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2386389"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..961)
                     /locus_tag="VFQ54_10430"
     CDS             complement(68..961)
                     /locus_tag="VFQ54_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165451.1"
                     /translation="MSGVEPNALNKAIPPDCEFAQLNLEAYVLNALDSSDQSRIDNHL
                     RWCDRDCPDQVREFRLIVDVLPFSVDIERAPSASVLTTLMARVEKTSTAVTEEPVARI
                     STRVTPPPVAPDGTTTDAPRRGPRRMIVPRWVASAFVAPLIVALIVVSAWGVGQYNRV
                     GDLKSQLQSQGSQIPAAMTGDDVRLYTTKPACADCSGSGQLGADPSENRAVLLAWDLN
                     PSETHEVWCVHSNGETSLVATLNVNKVGQVMQMIEFPRPISGYSEIQIVRHTDSSPEL
                     IFAVAEQDQESASPTDLVAGS"
     gene            complement(958..1566)
                     /locus_tag="VFQ54_10435"
     CDS             complement(958..1566)
                     /locus_tag="VFQ54_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0165452.1"
                     /translation="MSASGVTTDIDYGAMDDPQLVRLMAAEDPQALETLYTRYSRIVF
                     SFALRIVGDPAGAEELLQEVFFRAWQQARNFSEGRGSFVTWLLSITHNMAIDEIRKRQ
                     RRPQRADSADPVLVLSNVTADGPSVEEHALQSAWRDEIAGALKTLPDSQRQAIELAYF
                     HGLTQREIADQLGEPLGTIKTRMRLGMRKLREYLEKREVGVG"
     gene            complement(1822..3192)
                     /locus_tag="VFQ54_10440"
     CDS             complement(1822..3192)
                     /locus_tag="VFQ54_10440"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEU0165453.1"
                     /translation="MTSTTSYQDALAAIWARSGYDRGFISNPFAGDEAARLGLLRTQG
                     VLDRLGNPERDYRIVHVAGSKGKGSTTVTIDSILRATGQRVGRFTSPHLHSYRERIII
                     QNELISEADFAEATVETMRAVDAFERESPALGEVTAWELSTAMALLWFSRAACDYAVV
                     EVGMGGTLDATNIVTPVVSVITRLDFEHTAILGDTLPEIAANKAGIIKPGVPVVSAAQ
                     REDALAVILTKAAGTKSQVFVEGRDFDTSGSDASFSFTGPWGSIAGLHTLLAGQHQVE
                     NASLAIAATMAIEEVGAEEIRQALLDVTHPGRFEEITLPGGVPVVIDGAHSPVAARAL
                     VQTIRERHPNRRVLMVLGMLQDKDAANFVPELAAIVDKWLLAPPASPRAQTAAAVREA
                     VETTADVATFESVAEAIAAARSVAAARDMPLIVVTGSLTTVAEARVTLGLAHNDPAPT
                     SAPRVS"
     gene            complement(3320..>3891)
                     /locus_tag="VFQ54_10445"
     CDS             complement(3320..>3891)
                     /locus_tag="VFQ54_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU0165454.1"
                     /translation="PGLRAIGEVSCTGVHGANRLASNSLLEGLVFGLRAADAIAAQPA
                     PRSTVPLTADAATVELDAEGNGTADDADALRRDLQRVMSANVAVVRSAATLATADRIL
                     ADVATSASSIGNLTPRLLEVRNMAILAQALAASAAYREESRGSHFRADDPDRDPRLDG
                     LHQLVRHDGSEFERTFDALPRTDVATVGA"
BASE COUNT          658 a         1245 c         1263 g          725 t
ORIGIN      
        1 aacaggaccg cccctcttga ggggcggtcc gtgcctgttg accatcatgt ttgcgcccgc
       61 cgccgggtca cgatcccgcg acgaggtccg ttggcgatgc ggattcctgg tcctgctcgg
      121 cgaccgcaaa gatcagttcc ggcgaggaat cggtgtggcg gacgatctgg atctcgctgt
      181 agccggatat cggccgcggg aattcgatca tctgcatgac ctgcccgacc ttgttcacgt
      241 tgagcgttgc gaccagactg gtctcgccat tgctgtgcac gcaccacacc tcgtgcgtct
      301 ccgagggatt caggtcccag gcgaggagca cggcgcgatt ctcggacgga tcggcgccga
      361 gttgcccgct gccggagcag tccgcacacg ccggcttcgt cgtgtagagg cgaacgtcgt
      421 ccccggtcat cgcggcggga atctgactgc cttgcgactg cagctgcgat ttgagatcgc
      481 caacccggtt gtattgcccg acgccccagg cggagacgac gatgagggcc acgatcaggg
      541 gcgcgacgaa cgccgacgcg acccagcgcg gcacgatcat ccgccggggg ccgcggcgag
      601 gcgcgtctgt ggtcgtcccg tcgggagcca caggtggcgg cgtcacgcgc gtggatatgc
      661 gggccacggg ttcttccgtg accgccgtcg atgtcttctc gacccgggcc atcagcgtgg
      721 tgagaaccga ggctgacggt gcccgctcga tgtccaccga gaatgggagg acgtcgacga
      781 tgagacggaa ttcccggacc tggtccggac aatcgcgatc gcaccaacgc aggtgattgt
      841 cgatccggct ctgatcggag gaatcgaggg cgttgaggac gtaggcttca agattcagtt
      901 gagcgaactc acaatccgga gggatggctt tgttcagcgc gtttggttcc acaccactca
      961 ccctacccca acctcgcgtt tctcgaggta ttcgcgcaac ttgcgcatgc ccaaccgcat
     1021 gcgggtcttg atcgttccca acggttcgcc cagttggtcc gctatttccc gctgcgtcag
     1081 gccatgaaaa tatgccagtt caatcgcctg gcgctgagaa tccggcaggg tcttcagggc
     1141 gccggcgatc tcgtcccgcc acgcactctg gagcgcatgc tcctcgacac tcggtccatc
     1201 cgccgtcacg ttcgagagca caagcaccgg atcggcactg tccgctcgct gcggacgacg
     1261 ctggcgcttc cggatctcgt cgatggccat gttgtgcgtg atgctcagaa gccacgttac
     1321 gaacgacccg cgaccttcgc tgaaattcct tgcctgttgc caggcccgga aaaagacttc
     1381 ctgaagcaac tcttccgcac cggcgggatc gcctacgatt cggagggcga acgagaagac
     1441 gattcgcgag taccgcgtat acagcgtttc gagcgcttgc gggtcctcgg cggccatcag
     1501 gcggacgagt tggggatcat ccatcgcccc gtagtcgata tcggtggtca cgccagatgc
     1561 actcactgcg gacactcgac ccgactcagg cggggacgca tcaccatctg cctctgctta
     1621 aagctacgtt gccccggggt cgatggttct ctcgcacttg gggtccggag gggcgtctct
     1681 cgccagctat cagttttttc tcgatactgg ataaaccaaa tccgcatgga cgagtccttg
     1741 aatgtcaaat gttgagcgcg ggctcccgca agggcggtat cccaccagtc agtatgtcga
     1801 tcgtccttcc cccccgcggg tctaggatac ccgaggagcg ctcgtcggcg cgggatcatt
     1861 gtgagcgagt cccagtgtca cccgcgcttc ggccacggtc gtcagcgagc cggtgacgac
     1921 gatgagtggc atatccctcg ccgcggcaac cgatcgcgcg gcggcgatcg cttccgctac
     1981 tgactcgaac gtggcgacgt cggcggtcgt ttccaccgct tcacgcaccg ctgcagcagt
     2041 ctgggcccga gggctcgcgg gtggcgcgag aagccacttg tcgacgatcg ccgcgagttc
     2101 cggcacgaag ttcgccgcat ccttgtcctg tagcataccg agcaccatca acacccgtcg
     2161 attcggatgg cgttcgcgaa tcgtctgtac gagcgcccgt gcggccacgg gagaatgcgc
     2221 gccgtcgatc acgacgggga ctcctcccgg gagcgtgatt tcctcgaagc ggccgggatg
     2281 cgtgacatcc aggagtgcct gccggatctc ctctgctcca acctcctcga tcgccatcgt
     2341 tgcggcgatc gcgagcgagg cgttctcgac ctgatgctgc ccggcgagca gcgtgtgcaa
     2401 tcccgcgatc gagccccagg gacccgtgaa tgagaacgac gcatccgatc cgctggtgtc
     2461 gaaatcgcgt ccctccacga agacctggct cttcgttccg gcggctttcg tcaggatgac
     2521 ggcgagtgcg tcctcccgtt gagccgccga gacgaccggg acgccgggct tgatgattcc
     2581 cgccttgttg gcggcaatct ccggcagggt gtcgcccagg atcgcggtgt gctcgaaatc
     2641 gagccgtgtg atcaccgaga ccacgggagt gacgatgttg gtggcgtcaa gcgtgccacc
     2701 cattccgact tcgacgacgg cgtagtcgca cgccgctcgt gagaaccaga ggagcgccat
     2761 tgccgtggag agttcccagg cggtcacttc gcccagcgcg ggcgattccc gctcgaaagc
     2821 atcgacggcg cgcatcgttt caaccgtcgc ttcggcgaag tcggcctccg agatcaactc
     2881 gttctggatg atgattcgct cccgatagga atgaaggtga ggcgaggtga agcgcccgac
     2941 acgttgcccg gtcgcacgga ggatgctgtc gatcgtgacc gttgtcgatc ccttgccctt
     3001 gcttccggcg acgtgaacga tcctgtaatc gcgctcggga ttgccgagcc ggtcgaggac
     3061 gccctgcgtc cgcagcaggc cgaggcgggc agcctcgtcc ccggcgaacg gattgctgat
     3121 gaagccccgg tcgtagccgg aacgagccca gatcgcggcc agggcatcct gatagctggt
     3181 cgtggatgtc atcggacgag catctttcga tcgtggttga cgggtggaga caggcggcgc
     3241 gccggcaccg gaaccattat gcgcgacgga taaggaggaa cgatcccggt atcctgtgaa
     3301 tcttctgtga atctggcagc tatgcgccga ccgttgccac gtcggtccgg ggcaaggcgt
     3361 cgaaggtgcg ttcgaactcg gagccatcgt ggcggacgag ttgatgcagg ccgtcgagcc
     3421 ggggatcgcg gtccgggtcg tccgcgcgga agtggctccc gcgactctcc tcgcggtagg
     3481 cggcggatgc cgcgagcgcc tgggcgagga tcgccatgtt gcgaacctcg agcagtcgcg
     3541 gcgtgagatt cccgatcgac gatgcgctcg tcgccacgtc tgccaggatt cgatccgcgg
     3601 ttgcgagtgt tgccgccgag cggacgacgg cgacgttcgc actcatcaca cgctgaagat
     3661 cgcggcgcaa cgcatcggca tcgtccgcgg tgccgttgcc ttcggcatcg agttcgaccg
     3721 tcgccgcgtc ggcagtgagc ggaaccgtcg acctcggcgc tggctgcgcg gcaattgcgt
     3781 ccgcggcccg caggccgaag acgagcccct caaggagcga attgctggcg agtcgattcg
     3841 cgccgtggac gcccgtgcaa ctcacctcgc caatcgcccg gagtcccggt a
//
LOCUS       DASTMD010000707         2986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3191590, whole genome shotgun sequence.
ACCESSION   DASTMD010000707 DASTMD010000000
VERSION     DASTMD010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3191590"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VFQ54_10450"
     CDS             <1..1082
                     /locus_tag="VFQ54_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0165455.1"
                     /translation="MNIGLDGFRCDAVPYLFEREGTNCENLPETHQFLRRARRVIETE
                     YPGAIMLAEANQWPSDVVEYFGTEDEPEFHMAFHFPLMPRMFMAMRREQRTPIVEIME
                     STPAIADDNQWAIFLRNHDELTLEMVTDADRDYMYFEYAKDPRMRINVGIRRRLAPLL
                     ENGRRQIEMMNSLLMSMPGTPVLYYGDEIGMGDNIYLGDRNGVRTPMQWNSDRNAGFS
                     RADWAKLYFPVIMDPVYGYQAVNVEAQNRVPTSLLNWMKRIIAVRKRYQAFGRGTISF
                     LHPENFKVLVYLRRFENEIILCVVNLSRFVQYAEIDLGEFDGYQPVELIGEIHFPHIG
                     TLPYFITLGPHGFYWFRLEAPAPQV"
     gene            1125..2546
                     /locus_tag="VFQ54_10455"
     CDS             1125..2546
                     /locus_tag="VFQ54_10455"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165456.1"
                     /translation="MTFEERVGEALASSMSWLQAQRWFGDKSRTLVGVSVELFASVQL
                     RADRAALIVARCAFDTGADSLYFVPLVGFQNESTEAGAQPGRGHSVRDALTDTDFLAW
                     LLAGFQDRRAATATGTWTWSATHGSDRELALVDVYDSRVMDVEQSNTSIMFDHRIMAK
                     VFRRLQPGINPDLEVTRYLTETAEFAHVPRVYGVLELEHDGKTYVLGALQQFVTNVGD
                     GWGWLLEELRNLTRERRSALLADVSLLGRRTAELHVALGADTSDEAFRPDAMTSEQST
                     LLSGRVVEELERTIALLRARSAGTDEDLAELETKLRRRIGETKALVGTLQIRVHGDYH
                     LGQVLRTVDHDFAIIDFEGEPSRSIAQRREKFSPLRDVAGMLRSLDYAVGASTKAVSN
                     AQHIALIRDWGKDARASYLTGYRGAIGKGDPRLVPATDETFDAALHVLEIEKSLYEAR
                     YEMDNRPDWLSIPLGALFDLARL"
     gene            2626..>2986
                     /locus_tag="VFQ54_10460"
     CDS             2626..>2986
                     /locus_tag="VFQ54_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU0165457.1"
                     /translation="MLQSLILDALIVLILLLLAALGAYRGGLREMCTAAGLLLGVAIV
                     PEWATTWGNWVADKGDINHDAAHFMVAVGTLVAATMICGYGAGAAFAYRPGPGGRMYG
                     AILGLMVGVVFIGYVVNY"
BASE COUNT          549 a          939 c          940 g          558 t
ORIGIN      
        1 ggatgaacat cggcctggac ggttttcgct gcgacgccgt gccctacctc ttcgagcgcg
       61 agggcaccaa ttgcgagaac ctccccgaga cgcaccagtt cctgcgtcgt gcgcgacggg
      121 tgatcgagac ggaatacccg ggcgccatca tgctggccga ggccaaccaa tggccaagtg
      181 acgtggtcga gtacttcggc accgaggacg aacccgagtt ccacatggcc tttcacttcc
      241 cgctgatgcc gcgcatgttc atggcgatgc ggcgggagca acgcacgccg atcgtcgaga
      301 tcatggaatc gacaccggcg atcgcggacg acaatcagtg ggcgatcttc ctgcgcaacc
      361 acgacgagtt gacgctcgag atggtgaccg acgccgatcg cgactacatg tacttcgaat
      421 acgccaagga tccgcggatg cggatcaatg tcggcattcg ccgtcgtctc gccccactgc
      481 tcgagaacgg tcgccgtcag atcgagatga tgaactcgct gctgatgtcg atgccgggta
      541 ccccggtcct ctactacggc gacgagatcg ggatggggga caacatctat ctcggcgatc
      601 gcaacggcgt gcgaacaccg atgcagtgga acagcgatcg caacgccggg ttcagccgcg
      661 ccgactgggc gaagctttat ttcccggtga tcatggatcc cgtctacggg tatcaggccg
      721 tgaacgtcga ggcgcagaat cgcgtcccga cctcgctcct gaactggatg aagcgaatca
      781 tcgcggtgag gaagcgctat caggcgttcg gccgcggaac gatctcgttc ctccatcccg
      841 agaacttcaa ggttctcgtc tatctgcgac gcttcgagaa cgagatcatc ctctgcgtcg
      901 tcaatctcag ccgtttcgtg cagtacgcgg agatcgacct cggcgaattc gacggctacc
      961 agccggtcga actgatcggg gagatccact ttccgcacat cggaacgctg ccgtacttca
     1021 tcacgctcgg tccacacggc ttctactggt tccgcctgga ggccccggcg ccccaggtct
     1081 agattggctt ccccggatac cagggccatc gacgaggagg caagatgaca ttcgaagaac
     1141 gcgtcggaga agcgctggca tcctccatga gctggttgca ggcgcaacgc tggttcggcg
     1201 acaagtcacg aacgctcgtc ggtgtctcgg tcgagctctt cgcctcggtg cagctccggg
     1261 ctgatcgggc cgccctcatc gtcgcacgtt gcgcgttcga cactggcgcg gattcgctct
     1321 acttcgttcc actggtcggg tttcagaacg agtccaccga ggcgggggcc cagccgggtc
     1381 gcggacactc ggttcgcgac gcgctgaccg acaccgactt cctggcatgg ctcctcgcgg
     1441 gattccagga tcggcgcgcg gccactgcga ccggaacgtg gacctggtcc gccacgcacg
     1501 gcagcgaccg cgagctcgcc ctcgtcgacg tctacgattc gcgggtgatg gatgtcgagc
     1561 agagcaacac ctcgatcatg ttcgatcacc ggatcatggc gaaggtcttc cgccgtctcc
     1621 agccggggat caacccggat ctcgaggtca cgcggtacct caccgagacg gcggagttcg
     1681 cccacgttcc ccgcgtctac ggcgtactcg agctcgagca cgacgggaag acctacgttc
     1741 tcggagcact gcagcaattc gtgacgaatg tcggtgatgg ctgggggtgg cttctcgagg
     1801 aacttcgcaa cctgacgcgt gagcgtcgca gcgcgctcct tgccgacgtg agcctgctcg
     1861 ggcgccggac ggcggaactc cacgtcgcgc tgggagccga tacgagcgac gaggcgtttc
     1921 gcccagacgc gatgacgtcc gaacaatcga cgctgctctc tggccgcgtc gtcgaggaac
     1981 tggagcgaac gattgcgttg cttcgtgcac gcagtgccgg aaccgacgag gatctggccg
     2041 aactggagac gaaacttcgg cgccggatcg gtgagacgaa ggcactggtc ggaacgcttc
     2101 agattcgcgt gcatggcgac taccatctgg gtcaggtgct gcgcaccgtc gatcacgatt
     2161 tcgcgatcat cgacttcgaa ggggaaccgt cgagatcgat cgcacaacgt cgcgaaaagt
     2221 tctcgccgtt gcgcgacgtc gccggaatgc tgcgatcact cgactatgcg gtcggggcgt
     2281 cgacgaaggc ggtttccaac gcgcagcaca tcgcattgat tcgcgattgg ggaaaggacg
     2341 cgcgcgcgag ttacctgacc gggtaccgcg gcgcgatcgg caagggggat cctcgactcg
     2401 ttcccgccac cgacgaaacg ttcgacgccg ccctgcacgt tcttgagatc gagaagtcgc
     2461 tgtacgaagc tcgctacgag atggacaatc gacctgactg gctctcgatc cccctcgggg
     2521 cactcttcga tctggcccga ctctgagcga cacgtcacaa ttcgtgagca cccggcttct
     2581 tccgtccatc atccatcgac taccgggggc tagattggcc gaatcatgct gcagagcctg
     2641 atcctagacg cactgatcgt gttgatcctg ctgctgctcg ccgcgctcgg cgcgtatcgt
     2701 ggcggattgc gcgagatgtg cacggccgcc ggcttgctcc tcggggtcgc gatcgtgccg
     2761 gaatgggcca cgacctgggg gaactgggtc gccgacaagg gggacatcaa ccacgatgcc
     2821 gcccacttca tggttgccgt cggcaccctc gtcgcggcaa cgatgatctg cggctacggc
     2881 gcgggagcgg cgtttgccta tcgaccaggt cccggtggcc ggatgtacgg agcgatcctc
     2941 gggttgatgg tcggtgtggt ctttatcggc tacgtcgtca actacg
//
LOCUS       DASTMD010000708         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1404626, whole genome shotgun sequence.
ACCESSION   DASTMD010000708 DASTMD010000000
VERSION     DASTMD010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404626"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /gene="pyrH"
                     /locus_tag="VFQ54_10465"
     CDS             <1..511
                     /gene="pyrH"
                     /locus_tag="VFQ54_10465"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU0165458.1"
                     /translation="STIINSLILRGALTARNTHEVRVMTAIPIASVAEPYIRLRAIKH
                     LEKGYIVVFAAGIGQPFVTTDYPAVQRAIEIRAEAILAAKQGVNGVFTADPKRNPDAK
                     RFVSIAYDDVLARGLTVMDQSAFILARDHKIPIHVFDAGARNAMRDIVEGLPTGTYVG
                     PDAVTTLAD"
     gene            750..1418
                     /locus_tag="VFQ54_10470"
     CDS             750..1418
                     /locus_tag="VFQ54_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0165459.1"
                     /translation="MSTDPRIARTRKRLREALLALIEERDLVAINVQEITQSARVNRT
                     TFYLHYRSKEELLADAIDSLIEEIVVLNDEVRVLIESAPDLNVFTPPIDEPSLYDYFE
                     KRPRLFRKLFQAEIGISFVIKLQRFYEEQFIDIWNARHLVAAPGTPSAEFRARFASGA
                     ILAVISKNVVDDTPEKIQDYNLWGWELLLPLWSTMIVDSEISTPPRTTVAELGSSSSV
                     SPAR"
     gene            1415..2458
                     /locus_tag="VFQ54_10475"
     CDS             1415..2458
                     /locus_tag="VFQ54_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0165460.1"
                     /translation="MTPFTREDLSQALARPVGSFAITRQFDGRGAFVARIEVIFDEQS
                     DPEPLALVAKACMGTACAAEGIPEIAFFRDLAPRVPDLAIPAMLGWKIDHDETTSLLL
                     TEDLSETHRSPTAIEAPDILGVLVDEIARLHATFWDHSALASDSFAMPRANETRMPQA
                     YPAAVVRANAGILPAIAASFRVRFRNALSIEEDRLVSRLVASWPGMMEARTAQGNLTL
                     IHGDLHLLGNVLTSVGDGEDRIRIIDWADCKPGLGTHDIAYSTIAIQTLDRKHGDLAM
                     LQRYHRQLCELGVSTFSWEQCLWDYRFALLTNLLQCMLQESLTWFRTTAAIITEVGAD
                     SLLDETSDPPRML"
     gene            2517..>3233
                     /locus_tag="VFQ54_10480"
     CDS             2517..>3233
                     /locus_tag="VFQ54_10480"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEU0165461.1"
                     /translation="MADSPKVSFARVRPSETSTEEEIRAATVGEPTAVNGPITLVSYD
                     PSWPAFFAREDARIRSALAESDVIVELEHIGSTSVPGLSAKPIIDIQMIVINSSREET
                     YVPQLVAAGYVLRIREPEWEEHRVLKGTDPDVNLHVFSPGSTAARRHLRFRDHLRTHD
                     ADRDLYERTKRELAVQNWTYVQNYADAKNEVIDEILARAARSVTPYFARRTQLNSTSS
                     WVWRTLQDPPALPSGPSSISY"
BASE COUNT          659 a         1087 c          903 g          584 t
ORIGIN      
        1 gtccaccatc atcaacagcc tcatcctccg cggcgccctc accgcccgga acacgcacga
       61 agtccgggtg atgacggcaa tccccatcgc ctccgtcgcc gagccataca tccgcctgcg
      121 cgcgatcaag catctggaga agggctatat cgtcgtcttc gccgccggca tcggacagcc
      181 cttcgtcacc accgattacc ccgccgtcca acgcgcgatc gagattcgcg ccgaggcgat
      241 cctcgccgcc aagcagggcg tgaacggcgt cttcaccgcc gacccgaagc gcaatccgga
      301 cgcgaagcgc ttcgtctcca tcgcctacga cgacgtcctc gcgcggggcc tcacggtgat
      361 ggaccagtcg gccttcatcc tggcgcgcga tcacaagatt ccgatccatg tcttcgatgc
      421 cggtgcccgg aacgcgatgc gggatatcgt cgagggtctt ccaaccggca cctatgtcgg
      481 acccgacgcc gtgacaactc tcgccgactg aatcgctcca tacatgccaa aacgatgatt
      541 tcgccgagtg aggtcgcaaa ttgcgacctc actagctgtc accaacgcac aatcaatggc
      601 caagcaaggc ttcaccatta tcacaaatgc gttaatatat acgtagggaa gcaatcaatt
      661 ggtcctagac catgacggca atatctccta tgctccccta tggccggtca acggggacct
      721 gcctcgacgt atgaatatga aggcgtgccg tgtcgaccga tcccagaatc gcccgaaccc
      781 ggaaacgact ccgcgaagct ctccttgcct tgatcgagga acgcgatctg gtcgcgatca
      841 acgtgcagga gatcacgcag agcgcgcgcg tcaatcgcac cacgttctac ctgcactacc
      901 gcagcaagga agaactcctc gccgacgcca tcgattccct gatcgaggag atcgtcgtat
      961 tgaacgacga agtccgcgtc ctgatcgagt ccgcgccgga cctcaatgtc ttcacccctc
     1021 cgatcgacga gccatcgctc tacgactact tcgagaagcg accgcggctc ttccgcaagc
     1081 tcttccaggc ggagattggc atctcgttcg tcatcaagct gcagcgcttc tatgaagagc
     1141 agttcatcga tatctggaac gcacggcatc tcgtcgcggc gccgggaaca ccctcggccg
     1201 aattccgcgc ccgcttcgca tcgggtgcga ttctcgccgt catcagcaag aacgttgtcg
     1261 atgacacgcc cgagaagatc caggattaca acctctgggg ctgggaactg ctgcttccgc
     1321 tctggtcgac gatgatcgtg gattccgaga tctcgacacc accgcgcacg accgtcgcgg
     1381 aattgggctc atccagcagc gtctccccgg cgcgatgacc cccttcaccc gtgaagatct
     1441 gagccaggcg ctggcacggc ctgtcggctc cttcgccatc acgcgccagt tcgacggacg
     1501 aggggcgttc gtcgcgcgca tcgaggtgat cttcgacgag caatcggacc cggaaccgct
     1561 cgcgctggtc gccaaggcct gcatggggac ggcatgcgcg gccgaaggaa tcccggaaat
     1621 cgccttcttc cgcgatctcg ccccgcgggt tcccgatctt gcgatccccg ccatgctggg
     1681 ctggaagatc gatcacgacg aaacgacgtc actcctgctg accgaagacc tgagcgaaac
     1741 gcaccggtcg ccgaccgcaa tcgaagctcc ggatattctg ggcgtcctcg tcgacgagat
     1801 cgcgcggctc cacgccacgt tctgggacca ttccgcactc gcgagtgatt cattcgcgat
     1861 gccgcgcgcc aacgaaacac gaatgccaca ggcgtacccg gcggccgtcg ttcgggcgaa
     1921 cgcggggatt ctgcccgcga tcgccgcatc gtttcgcgtc cgcttccgga acgcgctgtc
     1981 gattgaagaa gaccgactgg tttcgagact cgtcgcaagc tggccaggga tgatggaggc
     2041 gcgcaccgcc cagggaaatc tgacgctcat ccacggcgac ctgcatctgc tcgggaatgt
     2101 cctgacgtcg gttggcgacg gtgaagatcg catccggatc atcgactggg cggactgcaa
     2161 gccgggtctt ggcacgcacg acatcgcata cagcacgatc gcgatccaga cactcgatcg
     2221 caagcacgga gatctcgcta tgctgcaacg ctatcaccgg caactttgcg agctcggcgt
     2281 gtcgacgttc agctgggaac aatgcctctg ggactaccgg ttcgcgctgc tcacgaatct
     2341 tctccagtgc atgctgcagg agagcctgac ctggttccga accacggcgg cgatcattac
     2401 ggaggttggc gcggactcgc tcctcgacga gacgtcagat ccaccccgaa tgctatgatg
     2461 caaggctgca tttccgccgg gcaggcagag cccgactatc gaaatggagc agcatgatgg
     2521 ctgattcacc gaaagtgtca tttgcccgcg ttcggccttc ggagacaagc accgaagagg
     2581 aaattcgcgc cgccaccgtc ggggaaccaa ccgcggtcaa cggaccgatc acgctcgtct
     2641 cctacgatcc gtcctggccg gcattcttcg cccgggagga cgcgaggatt cgctcagccc
     2701 tggccgaaag cgacgtgatt gtcgagctcg aacatatcgg atcgacctcg gttccgggac
     2761 tttccgcaaa gccgatcatc gatatccaga tgatcgtcat caattcatcg cgggaggaga
     2821 cgtacgtccc gcaactcgtt gcggccggat acgtccttcg tattcgcgag ccggaatggg
     2881 aggagcatcg ggtcctcaag ggaaccgatc ccgacgtgaa tctccacgtc ttctcaccag
     2941 gctcgacagc ggccagacgt catctccgct tccgcgatca cctccgcacg cacgacgccg
     3001 accgcgatct ctatgaacgg accaaacgcg agctggccgt gcagaactgg acttacgttc
     3061 aaaactacgc cgatgccaag aacgaggtga tcgacgagat tctggcccga gccgcccgga
     3121 gcgtaacgcc ctacttcgca cgccggactc agctcaactc gacctcgagc tgggtctggc
     3181 gcacgctcca ggatcccccg gcgcttccgt ccggacccag ctcgatctcg tac
//
LOCUS       DASTMD010000709         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_689046, whole genome shotgun sequence.
ACCESSION   DASTMD010000709 DASTMD010000000
VERSION     DASTMD010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689046"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFQ54_10485"
     CDS             complement(<1..290)
                     /locus_tag="VFQ54_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0165462.1"
                     /translation="MKFNYYGHSTFSIEADGTLVVVDPWFTGNPTNATSASEINPAAI
                     LITHAHNDHIGDALEIATRTGATIVSTGEIAGFLGSQGAKAVGGNFGGTVAFP"
     gene            complement(365..1957)
                     /locus_tag="VFQ54_10490"
     CDS             complement(365..1957)
                     /locus_tag="VFQ54_10490"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0165463.1"
                     /translation="MARLLIHGGPILTMDPANPLVEAVGIEEGRIAAVGSLSDVTSYL
                     GSRFDEIDLRGRLATPGLYDAHCHVMMTGFALNEIDVSAVAAPSIVVIQEKVRAVAQS
                     TPPGAWIVGQGYDQASLAEQRHPTRYDLDAVAPDHPVILWRSCHHILVANSAALRAGG
                     VSAATADLDDGVIDRDEHGEPTGVLREAATHLVTGVRDEPTEDDLVQAILAGGNEFRR
                     HGVTSAAEAGIRAPEQLRAYQRLANAGNLPLRTYLMMLIDETLDEMISLGIRSGFGND
                     MLRIGNAKLFSDGSIGGRTARMRKPYEGEPDNYGIWMIPPSEVKAKVLKAHTAGFQIG
                     IHAIGDAAIELILDAYAEAQAALPRPDVRHRIEHCSIVDEGLIARIAEQKVIPIPGTT
                     FLHYTRAAYEQNLGRDRFRYAYAMKTFAENGIIAAASSDAPVVPVNPMLGIATMVTRL
                     DRLGGDAWTEERVPVEEAIKAYTWNAAYASHEESIKGSLRPGLLGDVTIFETDLRSVA
                     PTELHEIKVDHTILNGDVVYAR"
     gene            complement(1966..>2468)
                     /locus_tag="VFQ54_10495"
     CDS             complement(1966..>2468)
                     /locus_tag="VFQ54_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165464.1"
                     /translation="ISLASESTTMVPELKDAASRLFDEEINGVADLLDSGRPVSSDEI
                     SRFGWSLTRSGGDFPVLNGSVDDDSDHVSIPTSAWWNDYAWWNPTAPQPPSSFLETLS
                     TSLQSVRTIGGLMTLVVHPHISGRPGFADTIVRFADEAIGAGDVWIPTATDLADWWRQ
                     RQFRAE"
BASE COUNT          409 a          749 c          849 g          461 t
ORIGIN      
        1 gggaacgcga cggtgccgcc gaaattgccc ccgaccgcct tggctccctg cgaaccgagg
       61 aagccggcga tttcgccggt cgagacgatc gtcgcgccgg tgcgggtcgc gatttcgagg
      121 gcatcgccga tgtgatcgtt gtgcgcgtgc gtgatcagaa tcgcagccgg attgatctcg
      181 gacgccgacg tcgcgttggt tggattgccg gtgaaccagg ggtcaaccac cacgagggta
      241 ccgtctgcct cgatgctgaa cgtcgagtgt ccgtagtaat tgaacttcac gtggatctcc
      301 tgtgtgtcgc ggattcgttg agcggcgtga catcgagtaa ggcgctcacg cgatgttcgc
      361 acgattatct cgcgtagacg acgtctccgt ttaggatcgt gtggtcgacc ttgatctcgt
      421 gcagttccgt cggcgcgacg gatcggaggt cggtctcgaa gatggtgacg tcgccgagca
      481 ggcccggccg cagcgatccc ttgatcgact cctcatgcga ggcgtaggcg gcgttccacg
      541 tgtaggcctt gatcgcctct tcgacgggga cgcgctcctc ggtccaggca tcgccgccga
      601 ggcgatcgag ccgggtcacc atcgtcgcga tcccgagcat cgggttgacg ggaacgaccg
      661 gggcatcgct gctggcggcg gcgatgatgc cgttctccgc gaaggttttc atggcgtagg
      721 catagcggaa acggtcgcga ccgagattct gctcgtaggc ggctcgcgtg tagtggagga
      781 aggtggtgcc cgggatcggg atcaccttct gttcggcgat gcgcgcgatc agcccctcgt
      841 cgacgatcga gcagtgctcg atccggtggc ggacatctgg ccgcggcaat gcggcctgcg
      901 cctcggcata ggcgtcaagg atcagttcga tcgcggcgtc tccaatcgca tggatgccga
      961 tctggaaccc tgccgtgtgc gccttgagga ccttcgcctt cacctcggac ggggggatca
     1021 tccagattcc gtagttgtcg ggttcacctt catacggttt gcgcatgcgg gcggtgcgac
     1081 cgccgatcga gccgtcggag aagagtttgg cgttgccgat ccgaagcatg tcattgccga
     1141 atccgctgcg aatgccgagc gagatcattt cgtcgagggt ctcgtcgatc agcatcatca
     1201 gatacgtgcg gagcggcagg ttgccggcat tggccagccg ctgataggcg cgaagttgct
     1261 ccggcgcgcg gattccggcc tccgcggcgc tcgtcacgcc gtggcggcgg aactcgttgc
     1321 cacccgccag gatcgcctga acgaggtcgt cctcggtcgg ctcgtcccgg acgccggtga
     1381 cgaggtgcgt cgctgcctca cgcaggaccc cggttggctc gccgtgctcg tcgcggtcga
     1441 tcacgccgtc atcgagatcg gcggtcgccg ccgagacgcc gccggctcgc agcgcggcgc
     1501 tgttggcgac gaggatgtgg tggcaggagc gccagagaat taccggatgg tcgggggcga
     1561 cggcgtcgag atcgtaccgt gttggatggc gttgttccgc gagcgatgcc tgatcgtatc
     1621 cctgcccgac gatccaggcg ccgggaggag tcgattgtgc gactgcgcgc accttctcct
     1681 ggatcaccac gatcgacggc gcggcgaccg ccgagacatc gatctcgttc agcgcaaagc
     1741 cggtcatcat cacgtgacag tgggcgtcgt agagcccagg ggtcgcgagt cgcccgcgaa
     1801 ggtcgatctc gtcgaatcgg gatccgagat agctggtgac gtcggacagt gaaccaacgg
     1861 cggcgatccg gccttcctcg atgccgacgg cttcgacgag cgggtttgcg gggtccatcg
     1921 tcaggatcgg accaccatgt atcaggaggc gagccatcgt ttctcctatt ccgcccggaa
     1981 ctggcgctgt cgccaccagt cggcaagatc ggtcgccgtc gggatccaga catcaccggc
     2041 gccgatcgcc tcgtcggcga agcgaacgat ggtatcggcg aaccccggcc gaccggagat
     2101 gtgaggatgg accacgagcg tcatcaggcc accgatcgtg cgcaccgact ggaggctcgt
     2161 cgagagcgtt tcgagaaagg acgacggcgg ctgaggagcc gttggattcc accacgcata
     2221 gtcgttccac caggcggagg ttggaatcga gacgtggtcg gaatcgtcgt cgacgctgcc
     2281 gttgagcacc ggaaaatcgc cgccggatcg ggtgaggctc catccgaacc gggagatctc
     2341 gtcgctcgag actggccggc cggaatcgag gagatcggca acgccgttga tctcctcatc
     2401 gaacagccgc gacgccgcgt ccttgagctc gggaaccatc gtcgtcgact cgctggcgag
     2461 actgatcg
//
LOCUS       DASTMD010000710         1598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2207709, whole genome shotgun sequence.
ACCESSION   DASTMD010000710 DASTMD010000000
VERSION     DASTMD010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2207709"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..797)
                     /locus_tag="VFQ54_10500"
     CDS             complement(15..797)
                     /locus_tag="VFQ54_10500"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEU0165465.1"
                     /translation="MTSTRWIDLNSDLGEGYGPFHVGHDVDADLFPLISSANVACGFH
                     GGDPRTIARTVERATAHGVAIGAHPSFPDLVGFGRRTIAASLAEIEADVLYQLGAVSA
                     FCTAVKTPLRHVKAHGALYNIAVKDRPVADAIAAAVAKFDSGLDFFALPGSELEAAGL
                     AAGLPVVREAFADRAYNADGSLVSRSLPGSVLTDPEVVAERMVRFVETGEIETIDGQI
                     LQLAAETICIHSDTPTAVPIARALRARFDTAGIGVRAHVAGV"
     gene            complement(823..1140)
                     /locus_tag="VFQ54_10505"
     CDS             complement(823..1140)
                     /locus_tag="VFQ54_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165466.1"
                     /translation="MRDPIAVTAVINAGTILATTPSSKKGGPLPRILLLVGLIIIVRT
                     FMRRTEHPIQIHFPPSLEATVTEPLKTDGPTLFSPPDAIDPLDVEETTADDTAPLSAH
                     SPN"
BASE COUNT          296 a          495 c          530 g          277 t
ORIGIN      
        1 gttcgaaggt ggaatcagac tccggcgaca tgggcgcgga cgccgatccc ggcggtatcg
       61 aagcgagccc gcagcgcacg cgcgatcggg acggcggtcg gcgtatcgga gtggatgcag
      121 atcgtctctg ccgcgagctg gaggatctgg ccgtcgatcg tctcgatctc gccggtctcg
      181 acgaagcgaa ccattcgctc tgccacgacc tccggatcgg taagcacgga tcccggcagc
      241 gagcgggaga ccagcgagcc atcggcgttg taggcgcggt cggcgaaggc ctcgcgaacg
      301 accggcagcc cggcggcgag acccgcggct tcgagttcac tcccgggcaa cgcgaagaaa
      361 tcgagcccgg agtcaaactt cgcgacggcg gcagcgatcg catccgctac cgggcgatcc
      421 tttacggcaa tattgtaaag cgcaccatgc gcctttacat gacggagtgg tgtcttcacc
      481 gccgtgcaga acgcggagac cgcgccgagt tggtaaagga catccgcctc aatctcggcg
      541 aggctggcgg caatcgtccg gcgaccgaag ccgacgagat cggggaagct cggatgcgcg
      601 ccgatcgcga cgccatgcgc cgtcgcgcgc tccacggtgc gggcgatcgt gcgcgggtca
      661 ccaccgtgga acccgcaggc gacattggca ctcgagatca gcgggaagag gtccgcatcg
      721 acatcatgcc cgacgtgaaa cgggccgtat ccttctccga gatcgctgtt gagatcgatc
      781 cagcgcgtcg acgtcatggg tacctttccg tgggataggc gatcagttcg gggagtgggc
      841 cgagagggga gccgtgtcat ccgccgtcgt ctcctcgacg tcgaggggat cgatggcgtc
      901 cggcggcgag aagagggtcg ggccgtcggt cttgaggggc tcggtgacgg ttgcctcgag
      961 cgaaggcggg aagtgaatct gaatcggatg ctcggtgcgc cgcatgaagg tgcggacgat
     1021 gatgatgagg cccacgagaa ggaggatccg gggaagtggc cctcccttct tcgacgaagg
     1081 cgtcgtcgcg aggatagtcc ccgcgttgat gaccgctgtg accgcgatcg ggtcgcgcat
     1141 gtctgatctc tcgaacaacg atcgtctcat catcgccccc ttgtctcgcc agcgttcttg
     1201 gcggcgcctt gcggcgaacg cgtcacgcga ttccatacgt gttccattat cacccaacgc
     1261 acgaacgtgc gcgggaatgc ggtgaagatt gcggcccggt ctggcgtccg cacctccctt
     1321 ggcaacatga acccgctgac ggagatctgt cgatccggtt cccgcgaatt cgatgaggtg
     1381 agcatgtgac cgtcgagatc gaggatgtca gccaaaggca actcaccccg cggttatgtc
     1441 gaagcaggag atcggggatg gtagacttac cattcgtctg tgacccgaat gtcaccgcgc
     1501 cccttgcctt cgcccgacca cgtccttcca agcgtcgcct tccaacggaa agatcactgt
     1561 gaccgccgac tccaccaccg cctcgttgac cgcttccg
//
LOCUS       DASTMD010000711         5232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1940294, whole genome shotgun sequence.
ACCESSION   DASTMD010000711 DASTMD010000000
VERSION     DASTMD010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940294"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFQ54_10510"
     CDS             complement(<1..402)
                     /locus_tag="VFQ54_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0165467.1"
                     /translation="MATVQFARIQKQYSGQDRPAVRDLSLDVRDGELLVMLGPSGCGK
                     TTALRMLAGLEEPDYGDIRLGGQSIVGLDPKDRDIALVFQQYALYPHLSVRDNMLYPL
                     KVRKMPSSDRIARVEQVAGMLGLGRLLKRKPA"
     gene            complement(468..848)
                     /locus_tag="VFQ54_10515"
                     /pseudo
     CDS             complement(468..848)
                     /locus_tag="VFQ54_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(876..1394)
                     /locus_tag="VFQ54_10520"
     CDS             complement(876..1394)
                     /locus_tag="VFQ54_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165468.1"
                     /translation="MANIAGTAPSAAANPANIAAASTGERVFHHPKKKRNLVEATIIH
                     VIFVFVIIFFLAPFLWLFEAAFETHADPFIRWPKAVTFSNFSYIFRELDFAQSLGNSV
                     FVSIISMVVTTFTVALAGYSLSRIEFRQKNWISYTILLLQTMPLSATMVPIYGLAREF
                     NLRNSYFGLVLV"
     gene            complement(1397..2302)
                     /locus_tag="VFQ54_10525"
     CDS             complement(1397..2302)
                     /locus_tag="VFQ54_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0165469.1"
                     /translation="MPRPPRRISAWRRNRAYVFGLTPAVILLAIFFLAPAIWAAYSSF
                     TNRALVGLDAANYHFVGLDNYRELLNDPDFSTVIWNSVVFVVGSAMIGQFVLGLGLAV
                     LIDLSEKRGYRIALVGYAAVLLAWVNPTVIAGFQWTAMFDYYYGTLNQTLQAVGLPPV
                     NWVGTYPMLSVIIVNIWRGTAFAMMIFMGALKTIPPHIYEAARIDGASGWQAFWDHTL
                     PNLRHIMLIEMLSLTISTFGTFILIQTLTNGGPAFKTEVIALYAYHTAFNTYEIGYGS
                     SIAIVMLGLNLIFGIVYIRAARPRV"
     gene            complement(2459..3895)
                     /locus_tag="VFQ54_10530"
     CDS             complement(2459..3895)
                     /locus_tag="VFQ54_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0165470.1"
                     /translation="MSNPRSLQGSISRRALLGQGAAVGAGLAVAGIAGASASSLQVGT
                     PTTGLTGEITVSYADTLGVKPKYVQQAADAVMAANPQAKINLTRDESDDSFTKLLLAL
                     DSGDSPDVIHTGGGSAGQLVDAGYVDPLDDYVSQWADWQYYPASVRSGVTYNGHVYSI
                     PYGLDTRFLYNRRDLLAKAGLGDDWAPANVQGILDAANAVKEAKLENVIPYALYAGVN
                     GGGGTTEHAYLPLLIAYGGSLQDASGKWVVAGDAIQKALQFIKDAYITDGLVPSEVLT
                     TTSPWTAMRQNIGADKLALLFEGGWVYGGYQTNVPDQLQDIGYLLHPTADGGPSFTIG
                     GPGTTWYLSAKSKNKQLAWEYIKAFNSRDIVADINIEDPHPVARTDSAALPQFQKVPF
                     LIDCTTSLQRAVFTPVSSDYAKVQLAIQKITGAIAAGQYDPDEGVSRLADEISRAIGK
                     DKVTDTPGPSVIPAGTPAPSPVASPIAS"
     gene            4410..>5232
                     /locus_tag="VFQ54_10535"
     CDS             4410..>5232
                     /locus_tag="VFQ54_10535"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0165471.1"
                     /translation="MVSVDTASSQSGSGKTKEDEGPVAQFVAVADGVVRPSETRSATP
                     RVVIIGGGFAGINVAKSLASAPVAVTLIDRRNHHLFAPLLYQVATAQLSPANIAQPIR
                     AITKGQKNLKVVLGEAENIDQEGHKVVLADGKEIPYDYLVVAVGATHSYFGHDEWEPV
                     APGLKTLEDAERIRRRILLAFERANVETDPEKRKALLTFIVIGGGPTGVELAGAIGEI
                     AHHTLAKEFDNIDPRQAKILLLEGLPRILPMFHEDLSAHATDDLKKFGVETRTGAL"
BASE COUNT         1008 a         1489 c         1819 g          916 t
ORIGIN      
        1 cgccggcttg cgcttgagga ggcggcccaa tcccaacatc cccgcgacct gttcgacgcg
       61 ggcgatgcga tcggacgatg gcatcttccg gaccttgagc ggatagagca tgttgtcgcg
      121 caccgagaga tgcggataga gcgcgtattg ctggaagacg agtgcgatat cgcggtcctt
      181 cggatcgaga ccgacgatgc tctgcccgcc gagccggatg tcgccgtagt cgggttcctc
      241 gagaccggcg agcatgcgga gcgccgtggt cttgccgcac ccgctggggc cgagcatcac
      301 caggagttcg ccatcgcgga catcgaggga gaggtcacgg acggccgggc ggtcctgacc
      361 cgaatactgc ttctggattc gcgcaaactg aacggttgcc atcgagagaa tccgtttccg
      421 ttatgacatg gccgcgtgtg ggcgtcgaat gcgtgggaat cggggcgcta catgcccttg
      481 gtcgaaccga cgaggccgtt cgccatcacc ttcctcgtca tgaagaagat gatcagcacc
      541 gggatgacat agaggacgcc catcgccgcg atcagctcgt agcgcacctc ggtgtaaccg
      601 ccgcgggtct ccatcgtccg gtagaaggcc agggggacgg tcgacttgtt cggatcgtcg
      661 atcaggatca gaacgagcat cacctccgcc caggcgctga gaaaggagag cccggcaacg
      721 acggcaagtc cggcgcgtgc cgtcggcagg acgacctcga accaggcacg cagcctggag
      781 cgaccatcga tccaggccgc ttcctcgagg gtgcgtggaa cctggtcgaa gaaccctttc
      841 atcagccaga ggaggaaggg gaggtcgagc gcgacctaga cgaggaccag gccgaagtag
      901 ctgttgcgga gattgaattc ccgggcgagt ccgtagatcg ggaccatcgt cgcggaaagc
      961 ggcatcgtct gcagcagcag gatcgtatag ctgatccagt tcttctggcg gaactcgatc
     1021 cgggagagcg agtagccggc gagcgcgacc gtgaaggtgg tgacgaccat cgagatgatc
     1081 gagacgaaga cgctgttccc gaggctctgc gcgaagtcga gctcacggaa gatgtaggag
     1141 aagttgctga aggtgactgc cttgggccag cgaatgaagg ggtccgcatg ggtttcgaag
     1201 gcggcttcga agagccagag gaacggcgcc agaaagaaga tgatgacgaa gacgaagatc
     1261 acatggatga tcgttgcttc gacgagattt cgtttcttct ttggatggtg gaagacgcgt
     1321 tcgccggtcg aggcggcggc gatgtttgcc ggattcgcgg cggcggatgg ggcggtaccc
     1381 gcgatgtttg ccatggtcag accctcgggc gcgcggcgcg aatataaacg atgccgaaga
     1441 tcagattgag cccgagcatc acgatcgcga tcgatgatcc gtagccgatc tcgtacgtat
     1501 tgaacgccgt gtgataggcg tagagtgcga tcacttcagt cttgaatgcc ggcccaccat
     1561 tggtgagggt ctggatcagg atgaaggtgc caaaggtcga gatcgtcagc gaaagcatct
     1621 cgatcagcat gatgtggcgg aggttcggca gggtgtggtc ccagaacgcc tgccaacccg
     1681 acgcgccgtc gatccgggcg gcctcgtaga tgtgcggcgg aatcgtcttc agggcaccca
     1741 tgaagatcat catcgcgaag gcggtgccgc gccagatatt gacgatgatc acggagagca
     1801 tcgggtaggt cccgacccag ttcaccgggg ggagaccgac tgcctgcagg gtctggttga
     1861 gcgtcccgta gtagtagtcg aacatcgcgg tccactggaa gccggcgatg acggttggat
     1921 tcacccaggc gaggagcacc gcggcatagc cgacgagcgc gatcctgtag ccgcgcttct
     1981 ccgagagatc gatcaggacg gcgaggccga gcccgagtac gaactgtccg atcatcgccg
     2041 agccaacgac gaagacgacc gagttccaga tcacggtcga gaagtcggga tcgttgagga
     2101 gctcgcgata gttgtcgaga ccgacgaagt ggtagttggc cgcgtcgagc ccgacgagcg
     2161 cgcgattggt gaagctggag tatgccgccc agatcgccgg tgcgaggaag aagattgcca
     2221 gcaggatcac ggctggagtc agcccgaaga cataggcgcg attgcgtcgc cacgcgctga
     2281 tgcgtcgcgg cgggcgtggc aagggatcgt ctcgtgtcag gggcggggcg aatccggggg
     2341 cgtcagcgga ggcatttgag gagcgatcga tcacgggtcg cctcgtggat gcgcgggaag
     2401 cggggcgccc cggtcgggcg ctccgcttct ccggacagat aggtatcgac gaacgcggtt
     2461 atgaggcgat cggcgatgcg acgggagagg gcgccggggt cccggcgggg atcacgctcg
     2521 gacccggggt gtcggtgacc ttgtccttgc cgatcgcgcg gctgatctcg tccgcgaggc
     2581 gggagactcc ttcgtccggg tcgtactgac cggcggcgat ggcgcccgtg atcttctgga
     2641 tcgcgagctg gactttcgcg tagtcggagc tgaccggcgt gaagactgca cgctgcagcg
     2701 acgtcgtgca atcgatcagg aacggcacct tctggaactg gggaagggcc gcgctgtcgg
     2761 tgcgggcgac gggatgtggg tcctcgatgt tgatgtcggc gacgatgtcg cgggagttga
     2821 aggccttgat gtactcccag gcgagttgct tgttcttgct cttggcgctg aggtaccagg
     2881 tggtgccggg gccgccgatc gtgaaggacg gcccgccgtc tgccgtcggg tgcaggaggt
     2941 agccgatgtc ctgcagctgg tcggggacat tggtctggta accgccgtag acccagccgc
     3001 cttcgaagag aagggcgagc ttgtcggcgc cgatgttctg ccgcatcgcg gtccacgggc
     3061 tcgtcgtggt aaggacctcg gacgggacga gtccgtccgt gatgtaggcg tccttgatga
     3121 actggagcgc cttctggatc gcgtcaccgg cgacgaccca cttgccggac gcgtcctgga
     3181 gcgatccgcc gtaggcgatc aggagcggca ggtaggcatg ctcggtcgtg ccgccgccgc
     3241 cattcactcc ggcatacagc gcgtagggga tcacgttttc gagcttggct tccttgacgg
     3301 cgttcgccgc atcgaggatg ccctggacgt tggcaggcgc ccaatcgtcg ccgagaccgg
     3361 ccttcgcgag caggtcacga cggttataga ggaagcgggt gtcgaggccg tacgggatcg
     3421 agtagacgtg accgttgtag gtcacaccag agcggaccga cgccggatag tactgccagt
     3481 cggcccactg cgaaacgtag tcgtcgagcg gatcgacata acccgcgtca accagctggc
     3541 ctgccgagcc gcccccggtg tggatgacgt ccggcgagtc accggaatcg agcgcgagaa
     3601 gaagcttcgt gaaggaatcg tcgctctcgt cacgggtgag attgatcttt gcctgcggat
     3661 tcgccgccat caccgcatcg gcggcctgct gcacgtattt cggcttgacg ccgagcgtat
     3721 cggcgtagga gaccgtgatt tcgccggtca gccccgtcgt cggggtgccg acctggagcg
     3781 aagaagcgga agcaccggcg ataccggcaa ccgccagacc ggcgccgacc gcagcacctt
     3841 gaccgagaag agcgcgccgg gaaatcgagc cttggaggga ccgcggattc gacatgcgtg
     3901 gggtttcctt ctcttgtatc gaggcgcatc ggcaagatgg gacggacctc gatcgtaggg
     3961 caggtgaggc tcgcgcggag tatggcgggc cgccccgatc atgctcccga attttgcaca
     4021 agtggtgaac aaatcttgaa tggggccgcg cgatttgctg agaagatgtg cacggggatg
     4081 ttcgctacag agaaggtcac agccagaggg gagactgttc gcgtccgggg gcgacgaccg
     4141 ttcccaacga cgaagacagg aaatatttgc ctggctcgga taccggcata tgatctttat
     4201 cgaattgtca tattcaaatg ccgccggtag ttgcatgtaa ttgccgttcg ttgatactct
     4261 gccccatgaa cattctgcgg aagaagcccg ggatgggggg caccgccgaa accggaaatc
     4321 cgaggtgcgg gtccggacga accaggagtc cgcgggatga tccgcagatg gatcttttcg
     4381 cgtggttcag cgagagagtg gagcgaagcg tggtttcagt tgacacggcg agcagccagt
     4441 cgggatcagg gaagaccaag gaagacgagg gaccggtcgc acagttcgtc gcggtcgcgg
     4501 acggtgtcgt tcgcccctcc gagacgcgaa gcgccacgcc gcgcgtggtg atcatcggcg
     4561 gcgggttcgc gggcatcaat gtcgcgaagt cgttggcgag tgcgccggtc gcggttacgc
     4621 tgatcgaccg ccggaatcac caccttttcg ccccgctcct ctatcaggtg gcgacggcgc
     4681 agctttcccc ggccaatatc gcgcagccga ttcgcgcgat caccaagggc cagaagaacc
     4741 tgaaggtcgt cctcggcgag gccgagaaca tcgatcaaga ggggcataag gtcgtcctcg
     4801 ccgatggcaa ggaaatcccc tacgactacc tcgtcgtcgc ggtcggggcg acccactcct
     4861 acttcggtca cgatgagtgg gagccggtcg cgccgggcct gaagacgctc gaggatgcgg
     4921 agcgcattcg tcgacggatc ctactcgcct tcgagcgggc gaacgtggag accgaccccg
     4981 agaagcggaa ggcgctgctg accttcatcg tgatcggcgg cggcccgacc ggggtcgaac
     5041 tcgccggcgc gatcggtgag atcgcccatc acaccctggc caaggaattc gacaacatcg
     5101 acccacgcca ggcgaagatc ctcctgctcg aggggcttcc ccgcattctg ccgatgttcc
     5161 atgaggacct ctcggcgcat gctaccgacg acctcaagaa gttcggtgtc gagacgcgca
     5221 ccggcgccct cg
//
LOCUS       DASTMD010000712         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2656447, whole genome shotgun sequence.
ACCESSION   DASTMD010000712 DASTMD010000000
VERSION     DASTMD010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2656447"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..623)
                     /locus_tag="VFQ54_10540"
     CDS             complement(90..623)
                     /locus_tag="VFQ54_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165472.1"
                     /translation="MLVFIPIGIVCSRLPRIGSATVGIVTSVRVVPSLALIVLFYPLF
                     GLGFRPTLFALVILAAPPIVLNTYTGLTQVDASVLEAARGVGMNELQVFLRVQLPLAL
                     PVMIAGLRTAAVEIIASATLASFVGVQTFGQWIVTGVSLLNNTYLLAGALPIMGLVLL
                     AEIFFGGLERLVTPRTA"
     gene            657..896
                     /locus_tag="VFQ54_10545"
     CDS             657..896
                     /locus_tag="VFQ54_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165473.1"
                     /translation="MRLKEFPELVRSVANEIKNVDKDVQFLAPSPQPVYERRDACFAT
                     AAFHNRTFVPKVNPECAVPPILDHDIHFGWRRNTK"
     gene            1188..1988
                     /locus_tag="VFQ54_10550"
     CDS             1188..1988
                     /locus_tag="VFQ54_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0165474.1"
                     /translation="MIFRKTREQSAPSTDKGIIQAQGVVKTYDTGTARLQALKGIDFA
                     VQRGEMVAVMGPSGCGKTTLLNCLSGLDEVDAGQIWLEGQDLAEMGDKKRTDYRARRM
                     GFIFQVYNLLPVLSSVENVELPLLVAGIRASDARRRALAALDTVGLADWAGHKPAELS
                     GGQRQRVTIARSLVNEPAIVWADEPTGALDSKSATDIMSLLRTLNNEKGLTLVVVTHD
                     PGVGRLCHRIVRMRDGEIVGEEIPDHTIAEALNHEVDTEVVGQGNLAH"
     gene            2091..>2338
                     /locus_tag="VFQ54_10555"
     CDS             2091..>2338
                     /locus_tag="VFQ54_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165475.1"
                     /translation="MDKLFGIPMTSIMIVLVILLGLCLLWVAYIALRRPVIFKLGVRN
                     IPRRKAQTSLIVVGLMLATLIITAALGTGDTLNHSVND"
BASE COUNT          487 a          769 c          682 g          400 t
ORIGIN      
        1 gatcttcacg atgttgtccc tccaactgag atcggcaatt cactgccgac aatgaccttg
       61 aggtggcgac cggccgtcgc caccactgat caggcggttc gtggcgtcac gagccgctcc
      121 aacccaccaa agaagatctc cgcgagcagc accaatccca tgatcggcaa tgcaccagcg
      181 aggagatagg tattgttcaa caacgagacg cccgtcacga tccactgccc gaacgtctgc
      241 acacccacga acgacgccag tgtcgcgctg gcgatgattt ccaccgccgc cgttcgcaga
      301 cccgcaatca tcacgggaag cgcgagcggc aactggacgc gaaggaaaac ctggagttcg
      361 ttcattccga caccgcgtgc cgcctcgagc accgacgcat cgacctgagt cagcccggtg
      421 tacgtgttga ggacgatcgg tggcgcggca aggatgacga gcgcgaagag cgtcggacgg
      481 aagccgagcc cgaacaatgg atagaagagc acgatcagcg ccaacgatgg gacgactcga
      541 actgacgtca cgatacccac cgttgcggat ccgattcgcg gcaaccgcga gcaaacgatt
      601 ccgattggta tgaagaccag catcgcgacc aggaccgcac tcaggctcaa ccgaagatgc
      661 gcctcaagga gttcccggaa cttgtccggt cggtcgcgaa tgaaatcaaa aacgtcgaca
      721 aagatgtgca atttctggcg ccctcgccgc agcccgtcta cgagcggcgt gacgcctgtt
      781 tcgccaccgc cgctttccac aatagaacgt tcgttccgaa agtcaacccc gaatgcgcgg
      841 tgccgccaat attggatcac gacatccact tcggatggcg acgaaacacg aaatagcggc
      901 cttactcgac acttgtctat tcctgtccgg gaatcatcaa catacggaat tcgtcatgcg
      961 ttcactccac gatttcttcg cggagcgttc acgggcccgt cacacttttg acgagattga
     1021 aatccggcgg gccgttatct tggagtcatc gcctgggatt tcgcgttgtc gaacctgttc
     1081 ccggggagaa acgtcacgaa cgctcgccgg gccgggtcgc tcccgcatgg caccgagcag
     1141 gacgccgcat gccattgcca cacaacccac gtgaggagac cccacgcatg atcttccgca
     1201 agacaaggga acagtccgcg ccatcgaccg acaagggcat cattcaggcg cagggcgtcg
     1261 tcaagaccta cgacacgggg accgccaggc tgcaggcgct caagggcatc gatttcgccg
     1321 tccagcgagg cgagatggtc gccgtgatgg gcccgtccgg ctgcggcaag acgacgttgc
     1381 tcaattgtct ttccggtctc gacgaggtcg atgccggcca gatctggctc gagggtcagg
     1441 acctcgccga gatgggagac aagaagcgca ccgattaccg agcacgccgg atggggttca
     1501 tcttccaggt ctacaacctg ctcccggtcc tcagctcggt cgagaacgtc gaactgccgc
     1561 tgctcgtcgc gggcatccgg gcgtccgacg cgaggaggcg agcgctcgcc gcgctcgaca
     1621 cggtcggtct cgccgactgg gcgggacaca agccggccga actttccggg ggccagcgcc
     1681 agcgcgtgac gattgcccgc tcgctggtca acgaacccgc catcgtctgg gccgacgagc
     1741 caaccggcgc cctcgattcg aagtcggcga ctgacatcat gagcctgctg cggacgctca
     1801 acaacgagaa ggggctgacg ctcgtggtgg tcactcacga tcccggggtc ggacggctct
     1861 gccaccggat cgtccgcatg cgcgacggcg agatcgttgg cgaggagatt cccgaccaca
     1921 cgattgccga ggccctcaac catgaggtcg acaccgaagt cgtcggtcag ggcaacctcg
     1981 cgcactagca ccgtcaccgg catcctcgtc cgcccgtcgc ggcagggatc gcgatctgga
     2041 tcaccgctca gatcgggact cctccatcct gataccggag tacattcacc atggataaac
     2101 tcttcggcat cccgatgacg tcgatcatga tcgtcctcgt gatccttctc ggtctgtgcc
     2161 tgctctgggt cgcctacatc gcccttcgcc ggcccgtcat cttcaagctc ggcgtccgga
     2221 acatcccgcg ccgcaaggcg cagacgtcgc tgatcgtggt cggcctgatg ctggcgacgc
     2281 tgatcatcac ggcggcgctg ggaaccggcg acaccctcaa ccactccgtc aatgacat
//
LOCUS       DASTMD010000713         1645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2029491, whole genome shotgun sequence.
ACCESSION   DASTMD010000713 DASTMD010000000
VERSION     DASTMD010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029491"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VFQ54_10560"
     CDS             <1..886
                     /locus_tag="VFQ54_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0165476.1"
                     /translation="MHACGHDAHTAMLLGVTRLLLDRRNEFAGTVKLLFQPAEEMHPG
                     GAKGMIADGALEDPHVDASLGLHVQSGTNAGLIGIRNGLGSANSDRFKITIQGKGGHA
                     SRPDWAVDPVVIGSHIVVAFQNLVARETDPMQPAVVTTTVLQAGEAFNVIPDTAELRG
                     TVRTFDQGVREHIAKRLPEIAEGVAKAMGGSAEATFMRGYPSIQNDPAMVEIVRAAAI
                     AAVGEENVFEPPVGMGGEDYSYFSLEVPSCFFYVGVRNEDRGITWGHHHPKFDIEEEG
                     MAGGLATMTGAILGYFNS"
     gene            1040..>1645
                     /locus_tag="VFQ54_10565"
     CDS             1040..>1645
                     /locus_tag="VFQ54_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0165477.1"
                     /translation="MVTTSSFVAEVDEILPGVIADRRWLHEHPELGFQEYETAAFVAQ
                     RLESLGVLNIETGIGGTGVTGMICGGQGDGMVVMVRADMDALAILEENAVDYASQNPG
                     VMHACGHDAHTAMLLGLARILIARKSEFKGAVKLLFQPAEEAHPGGAIAMIREGVLEN
                     PHVNASFGMHVMSDIPAGKIGVRAGLLTANSDRFTIRIQGKG"
BASE COUNT          289 a          549 c          554 g          253 t
ORIGIN      
        1 gatgcacgcc tgcggccacg acgcccacac cgcgatgctg ctcggcgtga cgcgcctcct
       61 gctcgatcgg cgcaacgagt tcgccgggac ggtgaagctc ctcttccagc cagccgagga
      121 gatgcatccg ggcggggcga aggggatgat cgccgacggc gcgctcgagg acccccacgt
      181 cgatgcctcg ctcggtctcc acgtccagtc cggcaccaac gccggcctga tcggaatccg
      241 caacggcctc ggctcggcga actccgaccg cttcaagatc acgatccagg gcaagggcgg
      301 ccacgcctcc cgcccggact gggcggtcga cccggtcgtg atcggctcgc acatcgtcgt
      361 cgccttccag aacctcgtcg cccgcgagac cgacccgatg cagcccgccg tcgtgacgac
      421 gaccgtgctc caggcgggcg aggcgttcaa tgtgatcccg gataccgccg agctccgcgg
      481 gaccgtccgc accttcgacc agggcgtccg cgagcacatc gcgaagcgtc tcccggagat
      541 cgccgagggc gtcgcgaagg cgatgggtgg ctcggcggag gcgacgttca tgcgcggtta
      601 cccgagcatc cagaacgatc cggcgatggt cgagatcgtc cgcgcggcgg cgatcgcggc
      661 ggtcggtgag gagaacgtct tcgagccacc ggtggggatg ggtggtgagg attactccta
      721 cttctcgctc gaggtgccga gctgcttctt ctacgtgggc gtccgcaacg aggatcgcgg
      781 catcacctgg ggccaccacc acccgaaatt cgacatcgag gaagaaggca tggccggtgg
      841 tctcgccacg atgaccggcg ccatcctcgg ctacttcaac tcgtaggatc gcaggaacat
      901 cacagcggga cccgaaagcc cgccgggccg tccccacccc gcagacgaac gacgacgttc
      961 gacgacaatc gctacggaag catcgatcgt acgtgctgcg cgacccgggg tcgcgcactt
     1021 catcagtcga ggaatgccca tggtcaccac gtcatcgttc gtcgcggagg tcgatgagat
     1081 cctgcccgga gtcatcgccg atcggcgctg gctgcacgag cacccggaac tcgggttcca
     1141 ggagtacgag acggcggcct tcgtcgccca gcgactcgaa tcactcggcg tcctcaacat
     1201 cgagaccggg atcggcggca ccggcgtgac ggggatgatc tgcggcgggc agggggacgg
     1261 catggtcgtc atggttcgtg cggacatgga tgcgctggcg attctcgagg agaacgcggt
     1321 tgactatgcg agccagaatc ccggcgtgat gcacgcctgc gggcacgacg cccatacggc
     1381 aatgctgctc ggtctcgcgc ggatcctgat cgcgcgcaag tcggagttca agggagcggt
     1441 gaagcttctc ttccagccgg ccgaggaagc gcatcccggt ggcgcgatcg cgatgatccg
     1501 cgagggcgtg ctcgagaatc cccacgtcaa tgcctcgttc ggcatgcacg tgatgtcgga
     1561 tatcccggcc gggaagatcg gggtccgcgc cgggctgctc acggcgaact cggaccgctt
     1621 cacaatcagg atccagggca agggc
//
LOCUS       DASTMD010000714         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1853148, whole genome shotgun sequence.
ACCESSION   DASTMD010000714 DASTMD010000000
VERSION     DASTMD010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853148"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFQ54_10570"
     CDS             <1..465
                     /locus_tag="VFQ54_10570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0165478.1"
                     /translation="LVEARYATRIAGTDTTRSFIIEIDGEPAGYIQCYRLVDEPEYLR
                     QLELPLPLHRNAVGTDLFIGEPARRNHGWGVPILRAFHRRIVFGEMRADPAIIAPEPG
                     NARAIKVYERVGFRWFRTVPIEDIEHPENSGDEYVMILSRSMFERHDGQRDE"
     gene            552..854
                     /locus_tag="VFQ54_10575"
     CDS             552..854
                     /locus_tag="VFQ54_10575"
                     /inference="COORDINATES: protein motif:HMM:NF037033.4"
                     /note="Related to clavulanate biosynthesis protein Cpe,
                     which has an isomerase-like N-terminal domain and a
                     beta-lactamase-like C-terminal domain; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cpe/LpqF family protein"
                     /protein_id="HEU0165479.1"
                     /translation="MRARFTHLAFILVALTGVLIPGEVAAQTPAPATPLPEGALGTQI
                     EWVMSILNGDTASTPESEITAHVDKSVLVQVPAKTLIASIAGLSTQYGPFRSNPVQ"
     gene            851..2023
                     /locus_tag="VFQ54_10580"
     CDS             851..2023
                     /locus_tag="VFQ54_10580"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0165480.1"
                     /translation="MITTKDMPPTTARFVIVGKNGMRFQASIAIDRWSGLITGLNFTV
                     AKPPATPAASPAATPAATNATDTDVSFQSGPDTIYGSFMQPKSGATGAAALIISGSGP
                     TDRNGNQSTMTVANTNLNIATTLAGVGISSLRYDKLGSGKTGLATHANGQGIDYSLFL
                     SEAQNAEKELLGQPGVDPSKLILIGHSEGALFALVLAQELAANGTPPAAVILVSPLSI
                     RYLDIIKEQVTAQYAQAVTAGQVTQAQSDAALKELDGIVTQLRTKGTVPATIETQALQ
                     QLFTPSSAAYLAEIDKIDPATIAASLPASLPVLVLHGTKDQQVSASDVAHLMSGFTKA
                     KNNSASLVTVPDANHLMKVVAGTPNAAIDYANPNLPFSPDAVKAIDGFLKATGLSS"
BASE COUNT          515 a          846 c          750 g          399 t
ORIGIN      
        1 ctggtcgaag cccgctacgc gacccgcatc gcggggaccg acaccacgcg ctccttcatc
       61 atcgagatcg atggcgagcc ggccggctac attcagtgct accgcctggt ggacgaaccg
      121 gagtacctcc ggcaactcga gctgccgttg ccgctgcatc gcaacgccgt cggtaccgat
      181 ctcttcatcg gggagccggc gcggcgcaat catggctggg gcgtcccgat cctgcgggcc
      241 tttcaccggc ggatcgtctt cggcgagatg cgggccgatc ccgcgatcat cgcccccgag
      301 ccggggaatg cgcgcgcgat caaggtctac gagcgggtcg ggttccgctg gttccgcacc
      361 gtcccgatcg aggatatcga acaccccgaa aacagcgggg acgaatacgt gatgatcctc
      421 tcgcgcagca tgttcgaacg gcacgatggg cagcgcgacg aatagcagat cgaaaataca
      481 gagccgaccg gcgagccgta gactgagaca agacctggtt atcgattcat tacgaaagag
      541 gtctagacaa tatgcgcgct cgattcaccc atctcgcctt catcctcgtg gcactcactg
      601 gcgtcctcat tcccggagaa gtcgccgccc aaaccccggc acccgccact ccgctcccgg
      661 agggagcact cggaacccag atcgaatggg tgatgtcgat tcttaacggc gacaccgcct
      721 cgacgccgga atccgagatc accgctcacg tcgacaagag cgtcctcgtt caggttccgg
      781 cgaagacctt gatcgcgtcg atcgccgggc tctcaaccca atacggtccc ttccgatcga
      841 atccggttca atgatcacga cgaaggacat gccgccaacg acggcccgct tcgtgatcgt
      901 cggcaagaac gggatgcggt tccaggcgtc gatcgcgatt gaccgttgga gtggcctgat
      961 caccggcctc aacttcacgg tggcgaagcc gcctgcgacg ccagccgcct ctcccgccgc
     1021 gacacccgcg gcgacgaacg cgacggatac cgacgtcagt ttccagagcg ggccggatac
     1081 gatctacggc tccttcatgc agccaaaatc tggcgcgacg ggagcggcgg cgctgatcat
     1141 ctccggcagc ggcccgaccg atcgcaacgg caaccagagc acgatgaccg tggcgaacac
     1201 gaatctcaac atcgcgacca cgctcgcggg agtcgggatc tcgtcgctcc gttacgacaa
     1261 gctgggatct ggcaagaccg gtctcgccac ccacgcgaat ggccagggca tcgactactc
     1321 gctcttcctg agcgaggcgc agaatgccga gaaggagctt ctcggccagc cgggggtcga
     1381 cccgtcgaag ctgatcctga tcgggcacag cgagggcgcg ctcttcgcgc tcgtccttgc
     1441 ccaggaactc gctgcgaacg ggacgccacc agctgccgtg atcctcgtct caccgctgag
     1501 tatccggtac ctcgatatca tcaaggaaca agtgaccgcg caatacgcac aggcggtcac
     1561 cgccggacag gtcacccagg cacagtccga cgccgccctc aaggaactgg acgggatcgt
     1621 cacgcagctc cgcacgaagg gcacggtgcc cgcgacgatc gaaacgcagg ctttgcagca
     1681 gctcttcacg ccgagcagcg cagcctatct cgccgagatc gacaagatcg acccggccac
     1741 gatcgcggcg agcctaccag cctcacttcc ggtcctcgtg ctgcacggaa cgaaagacca
     1801 gcaggtctct gcttccgatg tcgctcatct gatgtccgga ttcacgaagg cgaaaaataa
     1861 ctcggcgtcc ctggtcaccg tccccgatgc gaatcacctg atgaaggtag tcgcaggcac
     1921 gccgaacgcg gccatcgact acgcgaatcc gaacctgccc ttctccccgg acgcggtgaa
     1981 ggcgatcgac ggcttcctga aggcaaccgg cctctccagt taacggcaag cggcaagcgg
     2041 caagacacaa ggtcttgccc tacagacccg gggtggcgac atcgggtcga gcgatccgaa
     2101 ctgcgctgta ggacatcacg ggactccagg cccagctccc ctggacacgt cttcccgagc
     2161 ctgtcgagag atcggctggt taccacgtgc catacctaca acttcgttcc gtggaaacct
     2221 catgttgacc ccgtcgtgcc cccccccgcg ttcgtagggg cgagcctggt gctcgccctc
     2281 cgccgcaggt ggacttcaac cgaaacatcg gtctcaggag gcgttcccct gaaaacggat
     2341 cggcgaagcc gcggcgaccc gaaacggcga caagcgccgt ggggacggga atggcagcgc
     2401 gtcgttccac ggaaacggca cgcgccgcaa gattcgaatt gccggaggag acgagagggt
     2461 cgtagaatgg tagtgggata ttcccacggc ggatgcaagg acggacctcg
//
LOCUS       DASTMD010000715         1776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_243669, whole genome shotgun sequence.
ACCESSION   DASTMD010000715 DASTMD010000000
VERSION     DASTMD010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243669"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFQ54_10585"
     CDS             complement(<1..362)
                     /locus_tag="VFQ54_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEU0165481.1"
                     /translation="MGIEAFIVLLLIVANGLLSMSELAVVSSRSARLQQLADRGNRGA
                     TVAMQLAAEPNRFLSTVQIGITLIGILTGLFGGEAISLTIAEWLQGMFPAFAYSGVVG
                     KTLAVALITFLTLIFGELV"
     gene            879..1232
                     /locus_tag="VFQ54_10590"
     CDS             879..1232
                     /locus_tag="VFQ54_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0165482.1"
                     /translation="MRQRIGPTIKNRRHQHELSLNALADEAGISPSHLSRIERGLTVP
                     SYDVLDRIADALGSNLSALRHEEEHALEVDGDLDHIFAHIGLDEDARMELLQLSHETR
                     EALAKSLQRLQSGRL"
     gene            complement(1330..>1776)
                     /locus_tag="VFQ54_10595"
     CDS             complement(1330..>1776)
                     /locus_tag="VFQ54_10595"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU0165483.1"
                     /translation="DLSPLSPARARSYFDSVIMPASARGICWAIHERSTGEFIGSTAV
                     TDFRGNDTCLFRIVIGEKSAWGKGYGTEATSLVLAETFDVLDIAEVRLEVFSYNDRAR
                     GAYRRVGFRETGRHVEWVASHQRQLHVVEMACSRRDWTPPLLQQPR"
BASE COUNT          354 a          518 c          597 g          307 t
ORIGIN      
        1 accagttcgc cgaagatcag cgtcaggaag gtgatcaggg ccacggccag cgtcttgccg
       61 accacgcccg aataagcgaa ggccgggaac atgccttgca gccactcggc gatggtcagg
      121 ctgatggcct caccgccgaa caggccggtc aggatgccga tcagggtgat cccgatctgg
      181 acggtcgaga ggaaccggtt cggttcggcc gcaagctgca tcgcgaccgt agcgccgcga
      241 ttgccccgat ccgccagttg ctgcaggcgg gcggagcggg acgagaccac cgcgagttcc
      301 gacatcgaga gaaggccgtt cgcgacgatc aggagaagca cgatgaaggc ttcgataccc
      361 attgaatgaa gtggtccccc tggaacccgg tagtgagtct ggcgatcgca ggctgtcgtc
      421 gtgtcgttgc ccgaatgtcc gcgcgtcaac ggcacgaatc tggatgcgaa ctccggcgga
      481 cgtctttcgg gcccaacgcc cgaatcctcg cgaatgtctc tccactatac ggtatcgcga
      541 acagcgtcgc agacgacgga gagcgcgcgt gaccgggagt cgcaacgtcg gcgcggccgc
      601 acggggtgtc gtccgggccg cgaggggagg gaggatattc tttcctgcat tcaagcaacg
      661 gcccgggtga gaaagtggcg gataccccgg atggtgtggt tgctagacta ggagtaatgg
      721 acaagacggg aatatagtac tattactctc gtaaaggtga gcgacgtctc gtcccgctac
      781 ggtcaggtcg acctacacgg ggcatttgct ggagaacgtc ggacgggtct acagaggggc
      841 acatgatgtc gaccaacatt ggaggcggag gagggcccgt gcgacagcgg attggtccga
      901 cgatcaagaa tcgccgtcat cagcatgaac tctcactgaa cgccctcgct gatgaagccg
      961 gaatatcgcc atcgcatctg agtcgcatcg agcgcggact gacggtcccc tcgtatgacg
     1021 tgctcgaccg cattgcggat gcgctgggat cgaatctctc ggctctgcgg catgaggaag
     1081 agcacgcgct cgaagtcgat ggcgacctcg atcatatctt cgcgcacatc gggctggatg
     1141 aagacgcgcg gatggaattg ctgcagctga gccacgagac gcgagaggcg ctcgcgaagt
     1201 cactgcaacg gttgcagtcc gggaggctat aggccccgtt ctgcagtcga tctccacggt
     1261 ttccgatcga cacgaatcac gcctggccgg acgagtgcgt ctgtcgccag gcgtgttcgc
     1321 gttgcggcgt catcgcggtt gctggagcag gggcggcgtc cagtcgcggc gggagcacgc
     1381 catctcgacg acgtgcagct gccgctgatg gctggcgacc cattcgacgt gacgaccagt
     1441 ctcgcggaac ccgacgcgac ggtacgcgcc acgagcgcgg tcgttgtacg agaagacctc
     1501 cagccgcact tccgcgatgt cgagaacgtc gaacgtctcg gcgaggacga ggctcgttgc
     1561 ttccgtgccg tagccctttc cccatgccga cttttcgccg atgacgatgc ggaagaggca
     1621 ggtgtcgttc ccacgaaaat cggttacggc ggtcgatccg atgaactcac cggtcgatcg
     1681 ctcgtgaatg gcccagcaga tgccgcgggc cgaggcaggc atgatgactg aatcgaagta
     1741 gctccgcgcg cgggcgggtg agaggggact gagatc
//
LOCUS       DASTMD010000716         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1674578, whole genome shotgun sequence.
ACCESSION   DASTMD010000716 DASTMD010000000
VERSION     DASTMD010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674578"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(746..1732)
                     /locus_tag="VFQ54_10600"
     CDS             complement(746..1732)
                     /locus_tag="VFQ54_10600"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEU0165484.1"
                     /translation="MPEVKIKLAEVLPAKPNLMWELCKQLDVKHAVTGIPDGDGYPPA
                     WDFDYLLRIKQGFADAGFTLSVIESAPASIQEPIKLGLGDRDVHIDHFCQLIENMGRL
                     NIPVMCHNWMAGIGWLRTSMAERGRGGALVTGYDQAVLDNAPLTEWGIVTEEQLWERM
                     EDFLKRVVPVAEKAGVKLAVHPDDPPRSPVRGIGRILTSPENFQRVLDMAPSPNNGLT
                     MCQGNFSAMGVDVPETIRHFGRQGKIHFVHFRDVRGDRDKFVETFHDEGQTDMHETIR
                     AYADIGFDGPLRVDHVPTLAGEINDRPGYETMGRLYALGYTRGLIESVGKEA"
     gene            1954..2521
                     /locus_tag="VFQ54_10605"
                     /pseudo
     CDS             1954..2521
                     /locus_tag="VFQ54_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
BASE COUNT          436 a          810 c          825 g          450 t
ORIGIN      
        1 ggtcgggtgt cttccgaagc gtccgggggg ccgatggagc ctgatcgatc ttgccgttcg
       61 agccattggt ggcagccatt ggaatttgtc ctgacgtgaa gcgacgcggc gtggggcggg
      121 atagatgccc tggtaaacga atgtggaggc cgcgaagatg cgggcggcat cgcccgctcg
      181 gtcgatggtg cgaatcggaa tcatccgcaa ctgctgtgac catcatatcc ggtcaaggta
      241 gcaatcggcg gaccagcagt caaatgatga cggggtgatt ggatacgcct gcccgaaggc
      301 atccggccag tatagatgtt gcgcacgtat taacgctgga ggatgtgtcc gtgtgtcgac
      361 ggaaacggcg acgaatatcc ggatgatctc catctgtcgt tgggcttgtt gagggaccgg
      421 ccggcatcca cctgctcgcg gcacgacacc tccccacaca cgtcctccgg cgcaaccaac
      481 cgtcctcccg agcctgtcga gggaccagcc gtcatccgcg tgctcccgcg cgacgctgca
      541 cacaacacgt cctcccgagc ctgtcgaggg accgcatggg caccgcgcgc tccccggcac
      601 gacgcgtttt cctgggagcg gcagacggcg tggcgccgcc gcatcgaccc ggaacggcga
      661 caagcgccgt ggggacggga atggacgact cgccccgacg acgcggggga agaccttcac
      721 gattcccttc ccgagccgga aaggctcagg cttccttgcc gaccgattcg atcaggccgc
      781 gcgtatagcc gagcgcatag aggcgaccca tcgtctcgta ccccgggcga tcgttgattt
      841 cgcccgcgag cgtcggaacg tggtcgacac ggagcggtcc gtcgaatccg atgtccgcgt
      901 aggcgcggat cgtctcgtgc atgtccgtct gtccctcgtc atggaaggtc tcgacgaact
      961 tgtcgcggtc gccgcggaca tcgcggaagt gcacgaagtg gatcttcccc tggcgtccga
     1021 agtggcggat cgtctccggg acatcgacgc ccatcgccga gaagtttccc tggcacatcg
     1081 tgagaccgtt gttcgggctt ggcgccatgt cgagcacgcg ctggaagttc tccgggcttg
     1141 tcaggatgcg cccgatccca cggaccggcg accgcggcgg atcgtccggg tgaacggcga
     1201 gcttgacgcc cgccttctcc gcgaccggga cgacgcgctt caggaaatct tccatccgct
     1261 cccagagctg ctcctcggtg acgatccccc actccgtgag cggcgcgttg tcgagcacgg
     1321 cctggtcgta gccggtgacg agggcgccgc cgcgaccgcg ctccgccatc gaggtgcgca
     1381 accagccgat accggccatc cagttgtggc acatcaccgg gatgttgagc cgacccatgt
     1441 tctcgatcag ctggcagaag tgatcgatgt ggacgtcgcg atcgccgagt ccgagcttga
     1501 tcggttcctg gatcgacgcc ggcgccgatt cgatcacact cagcgtgaaa ccggcgtcgg
     1561 cgaatccctg cttgatccgc aacaggtagt cgaagtccca ggcgggtgga tagccgtcgc
     1621 cgtccgggat tccggtcacc gcgtgcttga cgtcgagttg cttgcagagt tcccacatca
     1681 ggttcggctt cgcgggcaga acctcggcga gcttgatctt gacctctggc atatcccctc
     1741 ctccgtgcgc cgatgcgcgg aaagaacgcg acaaacaggt tgttcgagag cttgggcgat
     1801 tgtcgacgca cccgccattc ctggcaacac tccccgcctg cctttcgtcg catcgcgggt
     1861 ccgggtagga ttgacagagc agcaaggaaa tcacgagatc cagtccagcc gcagccggtc
     1921 atcaggctca tgcgcgagcg aaggagatca cgtatgtcgt tggagtccgt ctttaccgcc
     1981 gaggcttcct cgatcgtctt cgggccggga gcgacccgcg aggtcggtgt ccgcgttcgc
     2041 gcggcgggat gcaggcgagt gctgctcgtc accgatccgc gaattgcccc gctcccgccg
     2101 gtcgcgacgg caaaggaggc gctgatagcc gcgggtgtcg agttcgaggt cttctccgac
     2161 gtgcgcgtcg aaccgacgga tagctcgtgg cagcatgcga tcgcgttcgc gaccgccggc
     2221 aacttcgacg gcttcgtcta ggtcggtggc ggctcgacga tcgacacggc gaaggtcgcg
     2281 aatctctatt cgacgcatcc ggcggagttc atggcctatg tctacccgcc ggtcggtgaa
     2341 gggcgccctg tcccggggcc gctgaggccg catttcgcga ttccgacgac ggcgggaacg
     2401 ggcagcgaga cgacggcgaa cgccgtcttc gatctgcagg cggcgcacac gaagacggcg
     2461 atatcccatc ggttcctgcg cccgacgcag ggaatcatcg accccgacaa cacgaggacg
     2521 a
//
LOCUS       DASTMD010000717         1625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_513462, whole genome shotgun sequence.
ACCESSION   DASTMD010000717 DASTMD010000000
VERSION     DASTMD010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513462"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFQ54_10610"
     CDS             complement(<1..571)
                     /locus_tag="VFQ54_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165485.1"
                     /translation="MIDDLGMLRRYLRFRCPDLSIVGLLRTFPATILLTSIVVACGTI
                     TWGQTRNEFKPTLDRLGFDYRLLTRGEFQRVITENFVQSTPGIHFSMVVMVATSLVLC
                     ELLAGSRNTVITFFVCHWAAAIGTILVLRLLSGFGMDRASALLSIPDAGSSASVHGAY
                     AVAAMMLPPRLATIAYGILLGITVVLLFQQ"
     gene            complement(564..>1625)
                     /locus_tag="VFQ54_10615"
     CDS             complement(564..>1625)
                     /locus_tag="VFQ54_10615"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEU0165486.1"
                     /translation="AAVSLIRPYVWKHAVYPAEQRHARSLIDSHGSASDDFFKYADDK
                     SFFFSGDGSGVVSYGVSTGTALVLGDPVARDAPSMRATLDEFLDFVDAHGWHPAFHHA
                     TPTHLQLYRDAGLIPLKIGAEAIVDLDAFTLAGKHGKNFRSTIARFTRDGYRAVFTEP
                     PIPAAKVAQLREVSNEWLTLDGRRERGFTLGRFSDEYVARSPIVSLESADGRIEAFMN
                     VIPDGVPGEITFDLMRHRVNAPNSSVDFVVLAAFRYAQERGFHRASLGMVPFAEVGTD
                     PNAALRERALARLTERFNHYFAATSLYAYKDKFHPTWEPRYLVYSGDAWLPSIGLAIS
                     RLTEKPLTAVSSSSGADHD"
BASE COUNT          312 a          469 c          592 g          252 t
ORIGIN      
        1 actgctggaa gagcagaacg accgtgatgc cgaggaggat gccatacgcg atcgtcgcga
       61 gccggggtgg cagcatcatg gcggcgacgg cgtaggcacc gtgaacggag gcggacgacc
      121 cggcatccgg aatcgacagg agcgccgaag cgcgatccat gccgaagccg gaaagcaggc
      181 ggagcaccag aatcgtcccg atcgccgcgg cccaatgaca gacgaagaat gtgatgacgg
      241 tgttgcgact tccggccagc agctcgcaga ggacgaggga tgtcgcgacc atcacgacca
      301 tcgagaagtg gatccccggc gttgactgga cgaagttctc ggtgatgacg cgctggaact
      361 cgcctcgggt gaggaggcgg tagtcgaagc cgagtcgatc cagcgtcggc ttgaactcgt
      421 tgcgggtctg accccaggtg atcgttccgc aggcgacgac gatcgatgtc aggaggatcg
      481 tcgccggaaa cgtccggagc agcccgacga tcgagaggtc ggggcagcgg aagcggagat
      541 accggcgcag catcccgagg tcgtcaatca tgatcggccc ctgacgatga tgacacggcg
      601 gtgagcggtt tttccgtcag ccgtgagatg gccagcccga tcgacggcag ccaggcatcg
      661 ccggagtaga cgagatagcg cggctcccag gttggatgga acttgtcctt gtaggcatag
      721 agcgaggtcg cggcgaagta gtggttgaag cgctcggtca ggcgggcgag tgctcgctcg
      781 cggagggcgg cgttcggatc ggttccgacc tcggcgaagg gcaccatgcc cagcgacgcc
      841 cggtggaatc ctcgttcctg cgcgtagcga aaggcggcga gcacgacgaa gtcgaccgag
      901 gagttcggtg cgttgacgcg atgccgcatc aggtcgaatg tgatctcgcc ggggacgccg
      961 tccggaatca cattcatgaa ggcctcgatc ctgccatcgg ctgactccag cgagacgatc
     1021 gggctccgtg ccacgtactc atccgagaat cggccgaggg tgaagccgcg ctcgcggcga
     1081 ccgtcgagtg tcagccattc gttcgagacc tcgcggagct gggcgacctt cgcggccggg
     1141 atcggcggct cggtgaagac ggcccggtac ccgtcgcggg tgaaccgggc gatcgtgctc
     1201 cggaagttct tgccgtgctt cccggcgagg gtgaaggcat cgagatcgac gatcgcctcg
     1261 gcgccgatct tgagcgggat caggccggcg tctcgataga gctggagatg ggtcggcgtc
     1321 gcatgatgga aggcgggatg ccagccatgg gcgtcgacga aatcgaggaa ctcgtcgagc
     1381 gtcgcgcgca tcgatggtgc gtcgcgggcg accggatcgc caagcacgag cgccgtgccg
     1441 gttgagacgc cgtagctgac gacgcccgac ccgtccccgg agaagaagaa gctcttgtcg
     1501 tcggcgtact tgaagaagtc gtccgacgcc gagccgtggc tatcgatcag gctccgcgca
     1561 tggcgctgtt cggcgggata gacggcgtgc ttccagacgt acggtcgaat cagcgagacg
     1621 gcggc
//
LOCUS       DASTMD010000718         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_244072, whole genome shotgun sequence.
ACCESSION   DASTMD010000718 DASTMD010000000
VERSION     DASTMD010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244072"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFQ54_10620"
     CDS             complement(<1..679)
                     /locus_tag="VFQ54_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165487.1"
                     /translation="MTDTIIPFPTQRPPVMEESSSPVWLHEEFAGLIDEATGVIGRVV
                     SSEKEPAGAHTFYFWASDEHLTLDVGHIVVSFSEQAAVIGVVDEPRRYSDLRSFLDDY
                     FDRRLELGLGEDSPTKRPEILVFTVNVLATKHLRKDVTSHRPAVTGPVFFATAKAIEY
                     ALGVQNFSGVPVPALMHTNGNYARDERGVVIDDENGNPRFQQTPLYLDEDYLLGPEAG
                     HANWTGQS"
     gene            complement(756..1862)
                     /locus_tag="VFQ54_10625"
     CDS             complement(756..1862)
                     /locus_tag="VFQ54_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165488.1"
                     /translation="MSIAARLPRTITEQVRAFRMENADLEMIDMRRSGSLELGIGANQ
                     VSANPFAPPTDFPMQIERLEGPIVGREIPVHHGPTGFRFFLDGAQKTLPVTRAGLVPI
                     SVALSTAGILERDERGEAQLAARAMRENQTWLFPLRTSSPELDRWQEWLSDRGADIRD
                     PLLGAKTDGDYQQLASHYGRLIELSQRMSGNLRALLEYDLLDMWRYELGPEYPDHWLI
                     VDGTLRRYSSNMIGLVKSFQMQHFIGEEAVALFELPQGYRTTAFRELADVHEVEDDIY
                     NGPVEKGERPTMWYQRFWDASGLDARHGLVRIEAPPEVNTTAQIDELASWLMTERLPR
                     PTMDPRWPTLLYPIHFLEEILKRRLAGMTTGWPS"
     gene            complement(2031..>2936)
                     /locus_tag="VFQ54_10630"
     CDS             complement(2031..>2936)
                     /locus_tag="VFQ54_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0165489.1"
                     /translation="HIRRYATIVQVVMLALALTLPASIARADGNAWVAATDAVNIRSC
                     PAESCPVLARVDLGQSLTVNGDLINGYLPVNYQGVNGYAYSLFVTDQTEDTWFTHGEA
                     GCKRVAFLFDIGIGDTPSRTVIDTLVDQKVTATMFPMGDWALENPDFLRQLAQDGFPI
                     GTHGQDSTFLTTASDDEIASNLTTSMTNIESVIGSPPILWATPYAADTDPRVRTVTAN
                     LGLVPVGWTVAADDYDTTATDDSVYQRVVGGVTDGAIVEFHLDGPATETSTALALPRI
                     IETLRGEGYTFVTVPEMANPCEAGS"
BASE COUNT          511 a          898 c          972 g          555 t
ORIGIN      
        1 cggattgccc cgtccagttg gcatgaccgg cctccgggcc gaggaggtag tcctcatcga
       61 ggtagagcgg cgtctgctgg aagcggggat tgccgttctc gtcatcgatc acgacgccgc
      121 gctcgtcgcg cgcgtagttg ccattcgtgt gcatcagcgc cggcaccggg acgccgctga
      181 agttctggac gccgagcgcg tattcgatcg ccttcgccgt cgcgaagaag accggtcccg
      241 tcaccgcggg tcggtgcgac gtcacatcct tgcggaggtg cttcgtcgcg agcacgttga
      301 ccgtgaagac gaggatctcg gggcgtttgg tcggtgagtc ttcgccgagc ccgagttcga
      361 ggcgtcgatc gaagtagtcg tcgagaaagg agcggagatc ggagtagcgg cggggttcat
      421 ccacgacgcc gatcacggcc gcctgttccg agaaggacac gacgatgtgc ccgacatcga
      481 gggtcaggtg ctcgtcggag gcccagaagt agaacgtgtg ggcgccggct ggttccttct
      541 cgctgctgac gacccgaccg atgacgccgg tcgcctcgtc gatcaggccc gcgaactcct
      601 cgtggagcca caccggcgac gagctttctt ccatgaccgg aggtcgctgg gttgggaagg
      661 gaatgatggt gtcggtcatg tcctgtactc cgatatcgcc acgttgatat ccacgaactc
      721 gttccggttg aagagaaagc aggacccaac catcgtcagc ttggccaccc cgtcgtcatt
      781 cccgctagcc gccgcttgag gatctcctcg aggaaatgga tcgggtagag cagcgttggc
      841 caacgcgggt ccatcgtcgg ccgcgggagc cgttccgtca tcagccacga ggctagttcg
      901 tcgatctgcg cggtggtatt gacctctggg ggtgcttcga tgcgcacgag gccatgccgg
      961 gcgtcgagac cactcgcatc ccagaaacgc tggtaccaca tcgtcggccg ctcgcccttt
     1021 tccactggtc cgttgtagat gtcatcctcg acctcatgga catccgcgag ctcgcggaac
     1081 gccgtcgttc gatatccctg ggggagttcg aagagcgcga ccgcctcctc cccgatgaag
     1141 tgctgcatct ggaacgactt gaccagcccg atcatattcg acgagtagcg ccgcagggtg
     1201 ccgtcgacga tgagccagtg atcgggatat tcagggccga gctcgtagcg ccacatgtcc
     1261 agcaggtcgt attcgagcaa tgcgcgcagg tttcccgaca tccgctggga gagttcgatc
     1321 agccggccgt agtgactcgc gagctgctga tagtcgccgt ccgttttcgc gccaagcagc
     1381 ggatcccgga tatcggcgcc gcgatcgctc agccactcct gccaacgatc gagttctggc
     1441 gaggaggttc tgagcgggaa gagccaggtc tggttctccc gcatcgccct ggccgcgagt
     1501 tgtgcctctc cccgttcgtc ccgctcgaga atgccggctg tcgacagcgc gacggaaatc
     1561 ggaaccagcc cggcgcgcgt tacggggagc gtcttctggg ctccgtcgag aaagaagcga
     1621 aagccggtcg gaccatgatg aacgggaatc tcgcgaccaa cgatgggacc ctcgagccgt
     1681 tcgatctgca tcgggaagtc tgtcggcggc gcgaacgggt tcgcgctgac ctgattcgcg
     1741 ccgatcccca gttcgaggga tccggagcga cgcatatcga tcatctcgag gtccgcgttc
     1801 tccatgcgaa atgcgcggac ctgttcggtg atcgtccgtg gaagccgggc tgcgatgctc
     1861 atctggatct cgttcctcgt cagaaggtgg ccgccgcgct gtcccggatt cctggaatgc
     1921 gtcccggttc tcctgccggc gctggactca cgttcgacga gaagagaaca cccgttcgat
     1981 tgtagggcat ttcgggcgta tcgaaaaccg gatctccgga aaaagcggga tcaggatccg
     2041 gcttcgcagg gattcgccat ctccggaacc gtcacgaagg tgtacccctc gccgcggagc
     2101 gtctcgatga tgcggggcag ggcgagtgcg gtcgacgttt ccgtcgccgg tccatcgaga
     2161 tggaattcga cgatcgcgcc gtccgtcacg ccgccgacga cgcgctgata gaccgagtcg
     2221 tccgtcgccg tggtgtcgta gtcatccgcc gccacggtcc agccgacggg cacgagtccg
     2281 aggttcgccg tcaccgtccg cacgcgggga tcggtatcgg cggcgtatgg cgtcgcccag
     2341 aggatcggcg gcgaaccgat cacgctctcg atattcgtca tcgatgtcgt gaggttgctg
     2401 gcgatttcat cgtcggacgc cgtcgtgaga aaggtcgaat cctggccgtg ggtgccgatc
     2461 gggaagccgt cctgggcgag ctggcggagg aagtcgggat tctcgagcgc ccagtcgccc
     2521 atcgggaaca tcgtggccgt gaccttctgg tcgacaagcg tatcgatcac cgtccgggat
     2581 ggcgtatctc cgattccgat atcgaagagg aaggcgacgc gcttgcaacc ggcctcccca
     2641 tgcgtgaacc aggtgtcctc ggtctggtcg gtgacgaaga gtgagtatgc gtaaccgttc
     2701 acaccctggt agttgaccgg gagatacccg ttgatgaggt caccgttcac cgtcagggat
     2761 tgcccgagat cgacccgggc aagcaccgga cacgactcgg cggggcagga gcgaatgttg
     2821 acggcatcgg tcgccgcgac ccacgcattg ccgtcggcgc gcgcgatcga cgcgggcagc
     2881 gtcagggcga gcgcgagcat gacgacctgt acgatcgtgg catagcgccg tatgtg
//
LOCUS       DASTMD010000719         4558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_513699, whole genome shotgun sequence.
ACCESSION   DASTMD010000719 DASTMD010000000
VERSION     DASTMD010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513699"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFQ54_10635"
     CDS             complement(<1..330)
                     /locus_tag="VFQ54_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU0165490.1"
                     /translation="MIEATVESIRVSLVTQHRVVILKEAEGERHLPIWIGSYEAEAIA
                     MELQGITPTRPLPYDLMQTMIAELHATVDRVLIIDLNDQVYYARILLDQGGRTIEIDS
                     RPSDAIAL"
     gene            complement(604..1398)
                     /gene="lepB"
                     /locus_tag="VFQ54_10640"
     CDS             complement(604..1398)
                     /gene="lepB"
                     /locus_tag="VFQ54_10640"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU0165491.1"
                     /translation="MTDHNSSAPEFRNLTLPSETPVDAVLPSADDPAKDGDGDAAPAT
                     PKKRPSMIREVIETLILALVIFVLVRAVVLNFKVDGHSMDSSFDNGEMLLVNRNAYFH
                     LGKSWLTDWIPGVHGDFYIFNPPKRGDVIVFNPPAAVGSDKPFIKRIIGLPGDTVETR
                     GGFVYVNGVKLDEPYIGGKVSKCNNNTVDCPPVTVPKGQIFVMGDNRTNSEDSRIFGT
                     VEESRIIGKAWVTYWPRDDIGVVPHYTYDNVPRPETTPTPEATPAN"
     gene            complement(1633..1908)
                     /gene="tatA"
                     /locus_tag="VFQ54_10645"
     CDS             complement(1633..1908)
                     /gene="tatA"
                     /locus_tag="VFQ54_10645"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU0165492.1"
                     /translation="MPFGIGAPELAIILVIVVIIFGVGKLPEIGGALGKGIREFKSES
                     GVDENGKPIDDAKLAKGSATTASTLTSSAKTESPKQTVAAGSRADEI"
     gene            complement(2143..2364)
                     /gene="secG"
                     /locus_tag="VFQ54_10650"
     CDS             complement(2143..2364)
                     /gene="secG"
                     /locus_tag="VFQ54_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU0165493.1"
                     /translation="MHAALNLSTIIVAIVLVVTILLQSKTSAFGGGMGGDSGSITKTR
                     RGFEKTLFQFTIGTAVVFVILCVMSGLLL"
     gene            complement(2467..3234)
                     /gene="tpiA"
                     /locus_tag="VFQ54_10655"
     CDS             complement(2467..3234)
                     /gene="tpiA"
                     /locus_tag="VFQ54_10655"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU0165494.1"
                     /translation="MERTPLVVANWKMNTDLTTALNLAEEAQDATEAVNPAIDVVVCP
                     PYPWIVPVSRALAGSRVSVGAQDVAAEEDGAYTGDVSAAMVAPWVRTVIVGHSERRAL
                     RGETDELVGRKLRAVLKHGLRPLLCLGETLAEREAGAAESVISRQFTTALDGLAPSEL
                     DDLSVAYEPVWAIGTGRAADQDDAEQMARHIRTLVASTVDPDHADRIRVLYGGSVNAA
                     NVARLTSGPDVDGALVGSASLAPGGFAGIIAAIATAN"
     gene            complement(3238..4422)
                     /locus_tag="VFQ54_10660"
     CDS             complement(3238..4422)
                     /locus_tag="VFQ54_10660"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU0165495.1"
                     /translation="MVKRSVTSADVAGKRVLCRVDFNVPMKDGRVADDTRIRAAIPTI
                     DWLREHGARIILCSHLGRPKGRIDDSLRLTAVGARLSRLIDLPVTTPHDVVGPEAQVA
                     VANLEDGEIVLLENLRFEPGEEENDPAFARQLADLAEIYVNDAFGAAHRAHASTEGVA
                     HLLPAYLGLLMQREVEALSSLLSHPPTPFVAIIGGAKVSDKIEILEHLVDRVSTLLIG
                     GGMANTFLLANGYEIGKSLAEPDQVDRALRIMKNAETSGVEIGLPVDIIVADSIDADE
                     GAVVDLESVGDRAIFDIGPQTAEIYASHLKKAQTVLWNGPLGVAENPAFASGTARVAE
                     AVAESQAFTCIGGGDSIAAVEALGLAGQIDHISTGGGASLEFLEGKTLPGIAVIPDVE
                     EA"
BASE COUNT          837 a         1370 c         1496 g          855 t
ORIGIN      
        1 gagtgcaatc gcgtcgctgg gccgactgtc gatctcgatc gtgcgaccgc cctgatcgag
       61 aagaatccgc gcgtaataga cctggtcgtt gaggtcgatg atgaggaccc gatcgaccgt
      121 cgcgtggagt tccgcgatca tcgtctgcat gagatcgtag gggagaggac gtgtgggtgt
      181 gatgccctgc agttccatgg cgatggcttc ggcctcgtag gaaccgatcc agatgggaag
      241 gtggcgttcg ccttcggctt ccttgaggat cacgacccga tgctgggtca cgagacttac
      301 ccggatactt tccacggttg cttcgatcat cgtcagccct ttccacggca cccgatggca
      361 agtcggcgac gatcgtcggc gtcgtccaac gacgccggtc acgagtgtct caggtgcgat
      421 ccgggacgcg cccggagccg cgtcccacta ccacaattct acacctcgcc tcaacgccgc
      481 aacagttggc aaccatgcga tgaacgggta gttgaggaac ggcatgatcg atctccgggt
      541 tcgcgatcgg gtatggagag gcccgtcgcg tgcacgcgac gggctctttg ctgaaccggg
      601 gatctagttg gcgggtgtgg cctccggtgt cggcgtcgtt tccggtcggg ggacgttgtc
      661 ataggtatag tgcggcacga ccccgatgtc gtccctcggc caataggtga cccatgcctt
      721 gccgatgatc cggctctctt ccaccgtccc gaagatgcgt gagtcctcgc tattggtgcg
      781 gttgtcaccc atcacgaaga tctggccctt gggaacggtg acgggcgggc agtcgaccgt
      841 gttgttattg cacttcgaga ccttgccgcc gatgtagggt tcatcgagct tcacgccgtt
      901 gacatagacg aagcctcccc gggtttcgac ggtatcgccg ggaagaccga tgatgcgctt
      961 gatgaacggc ttgtcactgc cgacggctgc cggcgggttg aagacgatga cgtcgcctcg
     1021 cttcggcggg ttgaagatgt agaagtcacc gtgcacgccc gggatccagt ccgtgagcca
     1081 ggacttcccg aggtggaagt aggcattgcg gttcacgagc agcatctcgc cattgtcgaa
     1141 gctggaatcc atgctgtgac cgtcgacctt gaagttgagg acgaccgcgc gcacgaggac
     1201 gaagatcacg agggcgagga tgagggtttc gatgacttca cgaatcatgg atgggcgctt
     1261 cttcggtgtc gcgggagcgg catcgccatc gccatccttg gctggatcgt ccgcggaggg
     1321 gagaacagca tccaccggcg tctcactcgg cagggtaagg ttgcggaact ctggtgccga
     1381 cgagttgtga tcggtcatgt tcaggatggt cctcatcagg tagcggactg gtgtccggac
     1441 gaacgccccg gcggatgata ccgtacgccg acgccaacga acgtcgtttg ggatgcggca
     1501 ggtacggcat ccgggcacgg agtgccgggt tcattcagta aacgcgaaga tggctggcag
     1561 cggagctatt ctccactgcc agccatctcc atctgcgtgc gttgcgcggg tgtgatttgc
     1621 tgggataagg acctagattt cgtccgcgcg gcttcctgcg gcgaccgttt gcttggggct
     1681 ctcggtcttg gccgacgagg tcagcgtcga agccgtggtg gccgaaccct tggcaagctt
     1741 ggcgtcgtcg atgggcttgc cattctcgtc gacaccggac tccgacttga attcacggat
     1801 gcccttgccg agcgctccac cgatttcagg gagcttgccg actccaaaga taatgacaac
     1861 gatcaccaag ataatggcga gctcaggtgc accgatcccg aatggcatga ctggaaatcc
     1921 tccacggacg ctggcagttc aggcgggtcc acgtccctcg ggacattccc atatggttct
     1981 gccaagcgtt aggcacattg tattcctcgg tatggccgat ttcaaacgtt tggaaacgtg
     2041 atgaaatcaa ccaatcgacg acgtacacac agaggagcgc atgtgccgga aaaccgcccc
     2101 tataggattt cgtcgaagca cgacccgctt tgcgaccccg ctctacaaga gcaggccgct
     2161 catcacgcac aaaatcacga acacgaccgc tgtgccgatc gtgaactgaa agagggtctt
     2221 ttcgaatccg cgtcgcgtct tcgtaatcga cccggagtca ccacccatgc caccaccgaa
     2281 cgccgacgtc ttgctctgga gcaggatcgt cacgacgagg acgatggcca cgatgatggt
     2341 cgagaggtta agcgctgcat gcaccggtct gactgatcct tcgtgtctgg cgggtcgaca
     2401 tcggcggtca ccaccgcgtc gacttcgtcg gtgaagagcg taccatcctc ttcacctgcg
     2461 ttcaattcaa tttgcggtcg cgattgccgc gatgatcccc gcgaatcccc caggagcgag
     2521 actggcgctg ccgacgagcg caccgtcgac gtcgggaccg gaggtcaggc gggcgacatt
     2581 cgccgcgttg acactccctc cgtagagaac ccggatccgg tccgcgtggt caggatcaac
     2641 cgtcgaggca acaagggtcc ggatatggcg cgccatctgt tcggcgtcat cctgatcggc
     2701 agccctgccg gtcccgatcg cccagaccgg ttcgtaggcg acgctcagat cgtcgagctc
     2761 ggagggcgcg agcccgtcga gcgcggtcgt gaactggcgg ctgatgacgg actcggccgc
     2821 gccggcctca cgttcggcaa gcgtctcgcc gaggcacagc agcggtcgca agccgtgctt
     2881 caggactgcg cgcagcttgc ggccgaccag ctcgtcggtt tccccgcgga gcgcccggcg
     2941 ctcggagtgt ccaacaatga cagtccggac ccagggggcg accatcgcgg cggagacgtc
     3001 accggtatag gcgccgtcct cctccgcggc gacatcctgg gcgccgacgg agacgcggga
     3061 gccggcgagg gcgcgggaaa caggcacgat ccacggatag ggaggacaga cgaccacgtc
     3121 gatcgccggg ttgaccgcct cggtggcgtc ctgagcctcc tcggccaggt tcagggccgt
     3181 ggtgagatcc gtattcatct tccagttggc aacgacgagc ggcgtgcgct ccacgatcta
     3241 ggcctcctcc acatccggga tcacggcgat gcccggtagt gtctttcctt caaggaactc
     3301 aagcgaggca cccccacctg tcgagatgtg atcgatctga ccggcgaggc cgagtgcctc
     3361 cacggccgcg atcgaatcgc caccgccgat gcaggtgaat gcctgggatt cggcgaccgc
     3421 ctcggcgacg cgggctgtgc cgctggcgaa ggccgggttc tcggcgacac cgagcgggcc
     3481 gttccagagc acggtctggg cctttttcaa atgactcgcg tagatctcgg cggtctgggg
     3541 accgatgtcg aagatggcgc gatcgccaac cgattcgaga tcgacgaccg cgccttcgtc
     3601 ggcgtcgatc gagtccgcca cgatgatgtc gacggggagg ccgatctcga ccccggaggt
     3661 ctccgcattc ttcatgatcc gcagcgcgcg gtcgacctgg tccggttcgg cgagcgattt
     3721 tccgatctcg tacccgttcg cgagcaggaa ggtgttcgcc atgccgccac cgatcagcag
     3781 ggtactcacg cgatcgacga ggtgctcgag gatctcgatc ttgtccgaga cctttgcgcc
     3841 gccaatgatc gcgacgaacg gagtcggggg atgggagagc aggctcgaga gggcttcgac
     3901 ctcgcgctgc atcaggaggc cgagatacgc cggcagcaag tgcgcgaccc cttcggtcga
     3961 cgcgtgggcg cgatgggcgg cgccgaacgc gtcgttgaca tagatctccg cgaggtcggc
     4021 gagttgccgg gcgaatgccg gatcgttctc ttcttcgcca ggctcgaagc ggaggttctc
     4081 gaggagaacg atctcgccgt cctcgagatt ggcgacggcg acctgggcct cgggtccgac
     4141 gacatcgtgc ggcgtggtca ccgggaggtc gatcaggcgg gagagacggg cgccgacggc
     4201 ggtgaggcgc aggctgtcat cgatcctgcc tttcgggcga ccgaggtgac tgcagaggat
     4261 gatccttgca ccgtgctcgc ggagccaatc gatggtcggg atcgcggcgc ggatgcgggt
     4321 gtcgtcggcg acccttccat ctttcatcgg cacattgaag tcgacacggc agagcacgcg
     4381 cttgccggcg acatcagcgg aagtgacgga gcgtttgacc atcagcagca acctttcaag
     4441 ggaaaccccc ggcgtcgcga tcggcgccgg gggtgggaca acgcatcgag ccggcaccag
     4501 cgacagcatc aacgcgtcaa gtatgagtga tagaagggaa gatgaggcga gtctagaa
//
LOCUS       DASTMD010000720         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1225786, whole genome shotgun sequence.
ACCESSION   DASTMD010000720 DASTMD010000000
VERSION     DASTMD010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225786"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFQ54_10665"
     CDS             complement(<1..549)
                     /locus_tag="VFQ54_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEU0165496.1"
                     /translation="MSHQRIGIIAALSKPEAAVLGAKITAWLEANGHTAIQESALGTT
                     PIAEVDAIVVLGGDGLMMRAGNTYPDVPLLGINFGKVGFLALVEQQDWQRALEALVSG
                     AFTIETSSTLAATLTRDGQTIDEGWAINDVVIRGGNRMVDVEVYIDGYWVNTFPGDGM
                     IVSTPRGSTAYCMAAGGPILTSG"
     gene            complement(555..1292)
                     /locus_tag="VFQ54_10670"
     CDS             complement(555..1292)
                     /locus_tag="VFQ54_10670"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165497.1"
                     /translation="MSKGMGDLGTPIGAPNSTTPIAIDAPFTLCVVPFRLASGRLEVG
                     TVRQSDDSITLPAGRPADDEPLDEGARRIINGHPGHGEEYLEQLYTFSPESGDDRLIV
                     VSYIALFREAACAQIAIPELRWSSLDGLRLSREIDRAVLQYAVVRLRAKLGYTNIAFH
                     LMPTTFTLTELQQAYESVLQHPVDKRNFRRRMMVPGTLEDTGEKRREGSHRPAALYRF
                     ASRDDQAAYLTPSWASERIDPLRDQGE"
     gene            1369..1442
                     /locus_tag="VFQ54_10675"
     tRNA            1369..1442
                     /locus_tag="VFQ54_10675"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1401..1403,aa:Gln,seq:ttg)
     gene            1590..>2065
                     /locus_tag="VFQ54_10680"
     CDS             1590..>2065
                     /locus_tag="VFQ54_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU0165498.1"
                     /translation="MQNQSQAGNPVSRPTVAVAVLAAGHGTRMKSQTPKHLHEVGGIP
                     IVERVIRAGLAVKPQQLVVVVSPRMADLPVRLGMEGQFSVAVQDEPRGTADAVRHALR
                     SIGSCDWIISLLGDSPLLTGETVSLLLEGAISTGCPVVVLTCQLPDAQSYGRIERD"
BASE COUNT          351 a          639 c          677 g          398 t
ORIGIN      
        1 gccactggtc aggatcgggc caccggccgc catgcagtag gcggtgctgc cacgcggtgt
       61 cgagacgatc atgccgtcgc cggggaacgt attcacccag tagccatcga tgtagacctc
      121 gacatccacc atgcggttgc cgccgcggat gacgacgtcg ttgatcgccc acccctcgtc
      181 gatcgtctga ccgtcccggg tcagcgtcgc ggcgagcgtc gagctcgtct cgatcgtgaa
      241 ggcgccgctg accagcgcct cgagtgcccg ttgccaatcc tgctgctcga cgagggcgag
      301 gaatcccacc ttgccgaagt tgatgccgag cagcgggacg tcggggtatg tattcccggc
      361 ccgcatcatc aggccgtcgc caccaagaac gacgatcgcg tcgacttcag cgatcggcgt
      421 cgtgccgagt gcggactcct ggattgcggt gtgaccgttt gcctccagcc aggccgtgat
      481 tttcgcgcca agcacggcgg cttccggctt gctgagtgcc gcgatgatcc caatcctctg
      541 atgtgacacc gctgtcattc cccttgatcc cggagcggat caatgcgttc tgatgcccat
      601 gacggcgtga ggtaggctgc ctgatcgtca cgcgatgcga aacggtagag ggccgccggg
      661 cggtggctcc cttcccgtcg cttctcgccg gtgtcttcga gcgtacccgg gaccatcatg
      721 cggcgtcgaa agttccgttt gtcgacggga tgttgaagga cgctctcgta ggcctgctgc
      781 aactcggtca gtgtgaatgt ggttggcatc aggtggaagg cgatgttggt atagccgagc
      841 ttcgcccgaa gacggacgac cgcatattgc agaacggcgc gatcgatctc gcgcgagagg
      901 cgaaggccat ccagcgaact ccaccggagc tcgggaatgg cgatctgcgc gcaggcggcc
      961 tcgcggaaga gcgcgatgta gctgacgacg atcagacgat cgtctcccga ttcggggctg
     1021 aatgtataga gttgctcgag gtattcctcg ccgtgaccag gatgtccgtt gatgatccgg
     1081 cgggcgccct cgtcgagcgg ctcgtcgtcg gcgggccgtc cggccggaag ggtgatggag
     1141 tcgtcggact ggcgaaccgt tcccacttcg aggcgcccgg aggcgagccg aaagggcacc
     1201 acacaaagcg taaatggcgc atcgattgcg attggcgtgg tggagttggg cgcaccaatc
     1261 ggtgtgccga ggtcacccat tcccttggac accttcattc cctgacgacg gtcggatggg
     1321 cgatctggcg cgaacatggc cgttcgcgta gacttgcgat ggccgcgatc gcgggtggtg
     1381 aaatggtatc acaagggtct ttggaacctt tattccaggt tcgaatcctg gcccgcgagc
     1441 caagctcctt catccgatga ccccttggca cccgcactga ctacgactcg gatcgctcca
     1501 tttcgacgct gcggacgtga tggtctccga cctttccgcg caagattcga tccagtcctc
     1561 gtgcgatcag tcggaagaga aaacccccag tgcaaaacca atctcaggct ggcaacccgg
     1621 tgtcacgacc gaccgtggcc gtcgccgtgc ttgccgcggg tcacggcacg cgcatgaagt
     1681 cgcaaacgcc gaagcacctc catgaggtcg gcgggattcc gatcgtcgag cgtgtgatcc
     1741 gggccggcct tgcggtgaag ccgcagcagc tcgtcgtcgt ggtcagtcct cggatggcgg
     1801 acctgccggt gcgactcggc atggagggcc agttttccgt cgcggtgcag gatgagccac
     1861 gggggacggc ggacgccgtt cgccacgcgc tgcgttcgat cggaagctgc gactggatca
     1921 tctccctgct tggtgacagc ccgcttctga cgggtgagac cgtctcgctc ctgctcgaag
     1981 gtgcgatctc gacgggatgt cccgtcgtcg tgttgacgtg ccagctgccc gatgcccaga
     2041 gctacggtcg gatcgagcgc gacca
//
LOCUS       DASTMD010000721         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_603412, whole genome shotgun sequence.
ACCESSION   DASTMD010000721 DASTMD010000000
VERSION     DASTMD010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603412"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VFQ54_10685"
     CDS             <1..1175
                     /locus_tag="VFQ54_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEU0165499.1"
                     /translation="LATTGRLLWRHLLAVGIEATNRLRANLVSLAETHANTNMPAFVG
                     GRAAQPTTLGHLLGGVIAPMASGERRMESAFAAINRSPLGAGQLAGDVILPDRERQAE
                     LLGFASPVPNTLDAVMSIEDFVETAEAIAAVMAPIRRLLGEIVAWVRTDPTSFVLTPE
                     WQTRPEPVNPLLVIPEQIELLMQELRAIEELCRAVTFRLRESAYGPIGSQAEFVLATA
                     SDLTRGVTVVLDRVGLLLTEGMLINRAFLGNRAGRGYTTGGDLAVFLMQEESIPPSAA
                     RVIAGMVLARVEEQGLEVSGITQDMIDSAAMLTIGREIKVEMETLGRFLAPRRFLDRR
                     QVLGAPAPPMTREWLVTERANLGRSESWLAGAVDSIDAAIARLETAIAAAATDEGE"
     gene            1551..>2075
                     /locus_tag="VFQ54_10690"
     CDS             1551..>2075
                     /locus_tag="VFQ54_10690"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEU0165500.1"
                     /translation="MRWPWLWSRWIALGIGIAAIVVGASLTLRPFTSLDALTAFIAIS
                     LILVGVGDLVADRIMPAMLPTRLIGLVLLATGVVAFTWPDATIRAVAIIAGIGILLGG
                     IVRIGIGLLPGIEDRYVLLVGGAAGVIFGVLALAWQDVTILAIALMVGPFVVIYGVGQ
                     IVRALRAPGPVVIRF"
BASE COUNT          297 a          653 c          756 g          369 t
ORIGIN      
        1 ggctggcgac gacgggccgc ctcctctggc ggcatctcct cgcggtcggg atcgaggcga
       61 cgaatcggct ccgggcgaat ctcgtctctc tcgccgagac acatgcgaat acgaacatgc
      121 ccgcgttcgt cggtggccgt gcggcgcagc cgacaacgct cgggcatctg ctcggcggcg
      181 tgatcgcgcc catggcatcc ggcgaacggc ggatggagtc cgcgttcgcg gcgatcaacc
      241 gctcgccgct gggagcggga cagctcgcgg gcgacgtgat cctccccgac cgcgaacgtc
      301 aggcggagct cctcggattc gcgtcgccgg ttccgaacac cctggatgcc gtgatgtcga
      361 tcgaggattt cgtcgagacg gcggaggcga tcgccgcggt catggcgccg atccgccggc
      421 ttctcggcga gatcgtggcc tgggtgcgga cggatccgac cagcttcgtg ctgacccccg
      481 aatggcagac ccgcccggag ccggtaaacc cgctgctcgt cattcccgaa cagatcgaac
      541 tgctgatgca ggagctgcgc gcgatcgagg aactctgccg cgccgtcacg ttccgcttgc
      601 gggagtcggc ctatggtccg atcggctcgc aggcggagtt cgtactggcg acggcgtccg
      661 atctcacgcg gggcgtgacg gtggtgctcg atcgcgtcgg gctgctgctg acggagggga
      721 tgctgatcaa tcgcgcgttt ctcgggaatc gcgccggtcg cggctatacg acgggcggcg
      781 atctcgccgt cttcctgatg caggaggagt cgatcccgcc gtccgcggcg cgtgtgatcg
      841 cggggatggt ccttgcgcgc gtcgaggagc aagggctcga ggtaagcggg atcacgcagg
      901 acatgatcga ttccgccgcg atgctgacga tcggacggga gatcaaggtt gagatggaga
      961 cgctggggcg cttcctggcg ccgcgtcgct tcctcgaccg ccgtcaggtc ctcggcgcgc
     1021 cggctccgcc gatgacgcgg gaatggctgg taacggagcg cgcgaacctc gggcgatcgg
     1081 agtcatggct cgccggggcc gtggattcca tcgatgccgc gattgcacgg ctcgagacgg
     1141 ccatcgccgc agcggcgacc gacgaggggg aatagggacc ggaacggacg ctgtgctccc
     1201 gggttcgggg gccgttcccg tcccggcgac cctcgtggtc gcctcggatc ggcggagccg
     1261 attcgtctcc cgaggagccc cggcgcggtc acgggggaac gatcctcggg tgaacgcgag
     1321 gcgggacagg acgtccggac tgcggtccgg cagcggcggt cccgctcctt gggcgagctc
     1381 aggacaagct cccgaacgac cgggcgcctg gcggaacacc gcatatgaat cgcatcgtca
     1441 gagccccaat gtttcccttt atccggataa gattgcacaa atggcaagat atcggggtgg
     1501 ttgccgcgat catcccgaat ccggggagtg gggagggtaa cgtgctaacg atgaggtggc
     1561 catggctgtg gtcgcggtgg atcgcgctag ggatcgggat cgcggcgatc gtcgtcggcg
     1621 cctcgttgac gctgcgcccc ttcacgtcgc tggacgccct gacggcgttc atcgcgatca
     1681 gcctgatcct ggtcggtgtc ggggatctgg tggccgatcg gatcatgccg gcgatgctcc
     1741 cgaccagatt gatcgggctc gttctcctcg cgacgggcgt ggttgcgttc acgtggccgg
     1801 acgccacgat ccgcgcggtc gcgatcatcg cgggaatcgg catcctgctc ggtggcatcg
     1861 tccggatcgg gatcgggctc ctcccgggaa tcgaggatcg ctacgtcctg ctcgtcggtg
     1921 gtgcggcggg tgtgatcttt ggtgtcctcg cgcttgcctg gcaggacgtg acgatcctcg
     1981 ccatcgcgct catggtgggg ccgttcgtcg tcatctacgg ggtggggcag atcgtccggg
     2041 cgctgcgcgc acccggcccg gtcgtgatcc gattt
//
LOCUS       DASTMD010000722         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1226466, whole genome shotgun sequence.
ACCESSION   DASTMD010000722 DASTMD010000000
VERSION     DASTMD010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226466"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1081
                     /locus_tag="VFQ54_10695"
     CDS             35..1081
                     /locus_tag="VFQ54_10695"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU0165501.1"
                     /translation="MRITIVGAGAMGSLIAARLSQASAASATPDAITSTAPITSQDDR
                     ITQGITRVLLYGRPSAHLDAISSHGLALTELDGGTHTIPIETSSDPIDVAGSDVVIVL
                     VKSWASAEAVAPLRPFLTRDSVVLTLQNGIGNAAMLRTALLDKGVRPHVWLGVTTQAA
                     LRTSPGHLIHSGDGITAIGRRNATANASIQTLAAHLTGAGWRTVAVDDIHRWVWRKLA
                     VNCAINPLTALGGVSNAAIRENPALAAAAAQIAAEVVAVAKAENVNIELAEVTAAIDE
                     VARNTGTNRSSMLVDLEHGMRTEIDAINGAVLASGRRLKIETPLNDLMVALVRAREKQ
                     VVEPSREHLASHAI"
     gene            1228..2133
                     /gene="panB"
                     /locus_tag="VFQ54_10700"
     CDS             1228..2133
                     /gene="panB"
                     /locus_tag="VFQ54_10700"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU0165502.1"
                     /translation="MTASPTSSVRPHLSVPAIRHHKVRSGATPLVMLTAYDAPTARLA
                     TAAGIDMILVGDSVGTAVLGYDSTVPVTLDEIIHHAKAARRGAPDAHIVVDLPFMTYQ
                     VSDEQAVTNAGRLLKEAGADAVKLEAGVTLASRVRAIASAGIPVIGHIGLTPQTAGAL
                     GGMRVQGRDLDGALGLIADADAVAAAGAYALVIEVVPADLARIITDRIAIPTIGIGAG
                     AGCDGQVLVWTDLAGLTAKPEEQAIAPRFAKHYVNLNASLAGAFAAFAGEVRSGAYPE
                     PEHGFTMKADVVAELEARLATETAE"
BASE COUNT          372 a          779 c          685 g          326 t
ORIGIN      
        1 agcgtcacgc atgcaaacat ggggaatgag gaccgtgcgt atcaccatcg tcggagcggg
       61 ggccatgggc tccctgattg ccgcccgact gtcacaggcg tccgctgcct cggcaacgcc
      121 tgacgcgatc accagtaccg cgcccatcac gtcccaggac gaccgaatca cccaaggcat
      181 cacacgcgtc ctcctgtatg ggaggccatc cgcccatctc gatgcaattt cctcgcatgg
      241 gctggcgctg accgagctcg acggtggaac gcacaccatc cccatcgaga ccagcagcga
      301 tcccatcgat gtcgccggca gcgatgtcgt gatcgtcctc gtgaagtcct gggcgtctgc
      361 cgaagccgtc gccccgctcc gtcccttcct gacacgcgac tccgtcgtcc tcacccttca
      421 gaacgggatc ggcaacgcgg cgatgctgcg aaccgcactg ctcgacaagg gggtgcgccc
      481 ccacgtctgg ctcggcgtga cgacacaggc ggcattgcgg acgagtccgg gacacctgat
      541 ccacagcggc gacggcatca ccgcgatcgg gcgccgcaac gcgaccgcca atgcgagcat
      601 ccagaccctc gccgcccacc tgacgggggc gggatggcgc acggtcgccg tcgatgacat
      661 ccatcgctgg gtctggcgca agctcgccgt caactgcgcg atcaacccgc tgacggcgct
      721 cggcggcgtc tcgaacgcgg caatccggga gaacccggcg ctcgccgcgg ccgcagcgca
      781 gatcgcggcg gaggtcgtcg ccgtggcgaa ggcggagaac gtcaacatcg agctggccga
      841 ggtgaccgcc gcgatcgacg aggtcgcccg caacacgggc accaaccgtt cgtcgatgct
      901 cgtcgacctc gagcacggga tgcggaccga gatcgatgcg atcaacgggg ccgtcctcgc
      961 gtcgggtcgc cgcctgaaga tcgaaacacc gctgaatgac ctgatggtgg cgctcgtccg
     1021 ggcccgcgag aagcaggtcg tcgaacccag tcgcgagcat ctcgcctcgc acgccatatg
     1081 atggtgggtc tggcggtggg acagcgacgt ccagcctccg atgaacgatc cgcgatcgac
     1141 gcttccttcc atatacatgc ccgatatgcc ggggccgcag atgacggctc gttacccgcg
     1201 tatttcgcca ggccaaaggc tcttctcatg accgcgtcgc caacgtcctc cgttcgtccc
     1261 cacctgagtg tcccggccat tcgccaccac aaggttcgat ccggcgcgac gcccctcgtc
     1321 atgctcacgg catacgacgc gccgaccgcg cgtctcgcga ccgccgccgg gatcgacatg
     1381 atcctcgtcg gcgactccgt cggcaccgcc gtcctcggct acgacagtac ggtgccggtg
     1441 acgctcgacg agatcatcca ccatgcgaaa gccgcgcgcc ggggcgcacc ggatgcccac
     1501 atcgtggtcg atcttccctt catgacctat caggtgagcg acgaacaagc ggtgacgaac
     1561 gccggtcggc tcctgaagga agccggcgcc gatgccgtca agctcgaagc cggcgtcacc
     1621 ctcgcgagtc gggtccgggc catcgcgagc gcgggaatcc cggtgatcgg acatatcggc
     1681 ctcacccccc agaccgccgg cgcactcggc gggatgcgcg tccaggggcg cgatctcgac
     1741 ggcgcccttg gcctgatcgc cgatgccgat gccgtcgcgg cggcgggcgc gtacgcactc
     1801 gtgatcgagg tcgttcccgc cgatctcgcg cggatcatca ccgatcggat cgcgatcccc
     1861 acgattggga tcggggccgg agccggctgc gatgggcagg tgctcgtctg gaccgatctc
     1921 gccggcctca ccgcgaagcc ggaggagcag gcgatcgctc cccgcttcgc caagcattac
     1981 gtcaacctca atgcgtcact cgcgggtgcc ttcgcggcct tcgccggcga ggtccgttcc
     2041 ggcgcctatc ccgagccgga acacggcttc acgatgaagg ccgatgtcgt cgccgaactc
     2101 gaggcgcgac tcgcaacgga gacggccgag tgagcgcccg gatcgtgacg aacccagccg
     2161 ac
//
LOCUS       DASTMD010000723         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2925049, whole genome shotgun sequence.
ACCESSION   DASTMD010000723 DASTMD010000000
VERSION     DASTMD010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2925049"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFQ54_10705"
     CDS             <1..351
                     /locus_tag="VFQ54_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU0165503.1"
                     /translation="GAMLGAAVWRYMITPVPQGLILPAGGAIGGMILMDLGSIFIQGN
                     GLWPEVLWLVVVGIAVFVLYRLVLAAAVKDDRRTLGEDGSRIVCPHCHQLTLRGAFCS
                     RCGEPLPQESTRPI"
     gene            complement(909..1355)
                     /locus_tag="VFQ54_10710"
     CDS             complement(909..1355)
                     /locus_tag="VFQ54_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165504.1"
                     /translation="MTGVLVVYDLNSGALQYEDDRLDLASPNGAPTVVLLLDAGAMAG
                     LDVPTLVRGRRSALHPSINLLVIAPETVGEFEDCAGSIRAPESEITRMARLFGVGSMP
                     ATVLLDAAGYVLWVSGGERTGNDWRPLTGGGDGRVSGAVGLGDLTN"
     gene            complement(1880..>2953)
                     /gene="aroF"
                     /locus_tag="VFQ54_10715"
     CDS             complement(1880..>2953)
                     /gene="aroF"
                     /locus_tag="VFQ54_10715"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU0165505.1"
                     /translation="QEGTEAAMLISLAQNAPESTRARLLAAARERGLNAQVMPDELGG
                     LVMGIGGQLGSDVSELPGVTRVQTIHKPYMMASLDARASSTVTVGDVMIGNGNQVVMA
                     GPCSVESEESQIEIAIAAKAAGAHILRGGAFKPRTSPYSFQGLGEEGLKHLAKAREIT
                     GMPIVTEVMDSEQVDLVAEYSDMLQIGSRNMANFSLLKRAGAAGRPILLKRGFSATID
                     EFLMSAEYLMAAGCHQVVLCERGIRTFDTTFRFNLDLNAVPGLKELTHLPVIVDPCHG
                     TGRRSMVKPMSLAGLAAGADGLMVEVHPDPDNALSDGYQTITPAELAKINRLGRAMLA
                     TLREAELEEDDLAAVATEMAIVA"
BASE COUNT          544 a          970 c          888 g          551 t
ORIGIN      
        1 ggcgcgatgc tcggcgccgc ggtctggcgc tacatgatca caccggttcc ccaagggttg
       61 atccttccgg cgggaggcgc catcggcggc atgatcctga tggatcttgg gtcgatcttc
      121 atccagggga acggcctctg gccggaggtg ctgtggctcg tcgtcgtggg gatcgccgtc
      181 ttcgtcctct atcgtctagt cctcgcggcc gccgtgaagg acgatcgccg gacgctcggt
      241 gaggacggga gccgaatcgt ctgcccgcac tgccatcaac tcacgctcag gggtgcgttc
      301 tgctcgcgat gcggcgagcc gctgccacag gagtcgaccc ggccgatctg agcgatcggg
      361 aggcgccgcg cgttctcatt gatggagaca aaggagcgcc ggtccggtgg tgtatcggac
      421 cggcgtaaag tgagctgaag catccatcca agtcgccacc ctgggaaagg tagacgtccc
      481 aagctgccgg gacttatgca ttcgctgcct gacaggaccc cgtcgaagac ctcgcatcgc
      541 gcgaagttgc atctgaaccg gtcgactgag gtggtgcatt cagtcttcca gattcgttta
      601 tcagtgaccg gagctgggca ctgaccgggt acctgggaag actggaaagg atgcgtttgg
      661 taacgcttcc agccacccac atcgccgggt gcggaatatt cggttgcccg attactctga
      721 aacacccggc gtcattcgcg atgcttcagt agggtcaccg taccgtcccc ttatagaatt
      781 gtcaagacag ttccggggac gctaggatcg atttcttcct cataaaagtg aacttcgtgc
      841 atagaattct ccatgtatcc cattcttcac aacgaagaat taagcactct accgtcacaa
      901 acatggcgtc aattcgtcag atccccgagc ccgacggcgc cactcacccg gccgtcccca
      961 ccgccggtca agggccgcca gtcgtttccg gtacgctccc cgccggagac ccagagcacg
     1021 tagccggcgg catcgagcaa aaccgtggcc ggcatcgacc cgactccgaa gagacgcgcc
     1081 atccgcgtga tctcgctctc gggggcccgg atcgagcccg cgcagtcctc gaattcgcca
     1141 accgtctccg gggcgatcac gagaaggttg atcgacgggt gcaacgcgct ccggcgcccc
     1201 cgcaccagtg tcgggacatc cagccccgcc atcgcgccgg catccaggag caggacgacc
     1261 gtcggcgccc cgttcgggct cgcgaggtca agccgatcat cttcgtactg caacgcacca
     1321 ctattcaggt cgtacaccac cagcactcca gtcacgctac gctgctcatc atcgagtcac
     1381 ggttccgccc tccgtggacc aacgtccggg agacgggatc gacgcgtgat tcccgggatt
     1441 gcgagatccc gcgggaacaa aatcaggacc ggagccgctt cccaggacgg ctccggcccc
     1501 gatctcaata gacccattgg taatgcccga ggcgggaccg cttcgcgaaa cccgtgcacc
     1561 tgcaataccg attgcttccg aacgcgtgcc gggccgaacc accacagcgg gcaactttca
     1621 gacttgccag ccaccccact cgataccacg ctgcacccgt acggatggct cattcatggt
     1681 gccagatcga caaggcgacg acatccagaa aattgcgtat ttcctgtggc gcaccggtac
     1741 gtaattgggg ctgcctcccc tacgtagtcc aatcatgtga cgctcgcgtg atggtctcgt
     1801 cgtggacgaa gctccggacg gacatcgcct tgcataggcg tggctcggga aatcgcgccg
     1861 gccgttggcc ccacgttcgt caggccacga tcgccatttc cgttgcgacg gccgcgaggt
     1921 catcctcctc gagttccgcc tcgcgaagcg tcgcgagcat cgcgcgacca agacggttga
     1981 tcttcgccag ttccgccggc gtgatcgtct ggtagccgtc gctcagcgcg ttgtcgggat
     2041 cgggatggac ttccaccatc aggccatcag cgccggcggc gagaccggcc aacgacatcg
     2101 gcttcaccat ggaacgacgt cccgttccat ggcagggatc gacgatgacc ggaagatgcg
     2161 tcagttcctt gagtccggga accgcgttga ggtcgagatt gaagcggaac gtcgtgtcga
     2221 aggtgcggat accgcgttcg caaaggacga cctgatggca gccagccgcc atcaggtact
     2281 cggcgctcat caggaattcg tcgatcgtcg ccgagaatcc tcgcttgagg agaatcggtc
     2341 gtcccgccgc gcctgcccgc ttcaggagcg agaaattcgc catgttgcgg gagccgatct
     2401 gcagcatgtc gctgtattcg gcgacgaggt cgacctgctc cgaatccatc acctcggtga
     2461 cgatcggcat gcccgtgatc tcgcgggcct ttgccaggtg cttgagaccc tcctcgccga
     2521 gtccctggaa cgagtacggt gacgttcgcg gcttgaaggc gccgccacgc aggatgtggg
     2581 cgccggccgc cttcgccgcg atcgcaatct cgatctggct ctcctcggat tcgacggagc
     2641 acggcccggc catcaccacc tgattgccgt tgccgatcat cacgtcaccg acggtgaccg
     2701 tgctcgaggc gcgcgcatcg agactggcca tcatgtacgg cttgtggatc gtctgcacgc
     2761 gggtgacgcc cggaagctcg gagacgtcac tcccgagctg cccgccgatg cccatgacga
     2821 gcccaccgag ttcatccggc atgacctgcg cgttgagacc acgttcccgc gcggcagcaa
     2881 gcagccgcgc gcgtgtcgat tccggtgcat tctgtgcgag agagatcaac atggcggctt
     2941 ccgttccttc ttg
//
LOCUS       DASTMD010000724         4536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2568104, whole genome shotgun sequence.
ACCESSION   DASTMD010000724 DASTMD010000000
VERSION     DASTMD010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2568104"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFQ54_10720"
     CDS             complement(<1..374)
                     /locus_tag="VFQ54_10720"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEU0165506.1"
                     /translation="MSKTTRATQELQKLKIAFTVHPHDYDPDADRKGLQAAAALGESP
                     HRVLKTLIADVDGKPVCVVVPSDREVSMKKLAAAFDGKTAKMIAPVDAERLTGYVVGG
                     ISPFAQKRRLPKAMERIAMDEE"
     gene            complement(396..1394)
                     /locus_tag="VFQ54_10725"
     CDS             complement(396..1394)
                     /locus_tag="VFQ54_10725"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEU0165507.1"
                     /translation="MIEATKPASRVLALLELLQDRPGISGPVLAEELGVTTRTIRRYV
                     ITLQEMGIPVEPMAGRTGGYWLRPGFRLPPLMFSPEEAIGLTIALLASRTTASTDLPR
                     PVTQALTKIERVLPRDLAERIESIRSSMLLPERNYPAESSFPNPNVLATLAQATLSSK
                     RCWMRYGRPDGDESAREVDCYGIVVHHGRWYLHGYCHLRKGTRTFRIDRIRRVDLLET
                     CFERPADIDVRAAVMASIALVRSDYQVELEIRGPIAMVRDYVYPDLAVLEEIDATTTR
                     LISSTETLDWFVRRMGMVPFSIRVISPPEMVDAFHEHAQRLLNISLAVGVREPVAV"
     gene            1550..2140
                     /locus_tag="VFQ54_10730"
     CDS             1550..2140
                     /locus_tag="VFQ54_10730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEU0165508.1"
                     /translation="MTESVAETTWTNETALLVIDVQKGVVVDGFDRDGVVSRIAGLIG
                     YARAHQVPVIYIQHEEPSYPPMARGGGDWEIVDEIAPNVGEPVIAKQYPDSFAETDLE
                     STLDDLGVRHLVICGAQSDACVRATTYRAMIEGYDVTLVGDAHTTSDRPYSGGTIPAE
                     QVINHVNIATPYIAYPGRAPRVVRQAELVTGEPVTI"
     gene            2238..2507
                     /locus_tag="VFQ54_10735"
     CDS             2238..2507
                     /locus_tag="VFQ54_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165509.1"
                     /translation="MLPFNPDIMNVAIAEQQERIRIHMLRAQARGFADDVNLLRRLIG
                     RALVWSGRRVGGPVVVAATTPESLAPVIDLGSHRLEADRNLERAA"
     gene            2713..3285
                     /locus_tag="VFQ54_10740"
     CDS             2713..3285
                     /locus_tag="VFQ54_10740"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEU0165510.1"
                     /translation="MEPVSNFIWRPWEEPGSEHLRIHRAPSGEMIADSQLVTVIDGAV
                     LRLSYRVELDANWRTLSVRVACEQPNGTAIGLALQSDGAGNWRDGSGLALPELGGCLD
                     IDIQATPFTNTLPIRRLNLAAEESEVIRVVYIPVPTLDVAPMKQQYTGIDDRHVRYES
                     IRSGFTRELEIDRDGFVVQYPGLFRRIWPI"
     gene            3365..3438
                     /locus_tag="VFQ54_10745"
     tRNA            3365..3438
                     /locus_tag="VFQ54_10745"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3397..3399,aa:Cys,seq:gca)
     gene            3552..3899
                     /locus_tag="VFQ54_10750"
     CDS             3552..3899
                     /locus_tag="VFQ54_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165511.1"
                     /translation="MSSIEEQPTTSTTIDESDSPTATSAALSEEEQRRRRSRAIAWGA
                     GIFLAFGLGLPIWYALDDWRAGLITAIVVGGLTVVGYLKAGSDHAEQRAERQARMYSR
                     EDQDDEDDDGSYN"
     gene            3933..>4536
                     /locus_tag="VFQ54_10755"
     CDS             3933..>4536
                     /locus_tag="VFQ54_10755"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0165512.1"
                     /translation="MSERDERFETDGDPEPAYMAWQRGPVLPHEVTTETRRGFDGHLL
                     HVRVDDVILPSGRMSRREIVEHPGSVVILPLTVDREVLLIRQWRHVTNRYQLELPAGV
                     IDPGEFFMQTAGRELIEETGHEAGDLRHLATIFMSPGYTNERTAFVLATGCRAIEREA
                     DVDEPMELIRRPLSSIADLLMPGNAAIEDAQTVLGLLWLLR"
BASE COUNT          819 a         1375 c         1532 g          810 t
ORIGIN      
        1 atctcctcat ccatcgcgat tcgttccatc gcctttggca ggcggcgctt ctgtgcgaac
       61 gggctgattc ccccgacgac atatcccgtc agccgctcgg catcgaccgg agcgatcatc
      121 ttcgcggtct tgccatcgaa ggccgccgcg agtttcttca tgctgacctc gcgatcgctc
      181 ggcaccacca cgcagaccgg cttgccgtcg acatcggcga tcagcgtctt caggacgcga
      241 tggggtgatt ccccaagcgc ggccgcggcc tggagaccct tgcggtcggc gtcggggtca
      301 tagtcgtggg gatggaccgt gaacgcgatc ttcagcttct ggagttcctg cgtcgcccgg
      361 gtggtcttgc tcatcgcttc cttgccatgc ctcgatcaga cggcgacggg ttcgcgcacg
      421 ccgaccgcga gcgagatgtt cagcaaccgc tgcgcgtgct cgtgaaaggc gtcgaccatt
      481 tccggcgggg agatcacacg gatcgagaac ggcaccatgc ccattcgccg cacgaaccag
      541 tcgagggttt ccgtcgagga gatgagacgc gtcgtcgtcg cgtcgatctc ctcgaggacc
      601 gcgagatccg gatagacgta gtcgcggacc atcgcgatcg gcccgcgaat ctccagttcg
      661 acctgatagt cgctgcggac gagggcgatc gacgccatga ccgcggcgcg gacgtcgata
      721 tcggcgggtc gctcgaaaca cgtctcgagg aggtcgacgc ggcggatgcg atcgatccgg
      781 aatgtccgcg tgcccttgcg gagatggcag tagccatgga gataccagcg tccgtgatgg
      841 acgacgatgc cgtagcaatc gacctcgcga gccgattcgt cgccgtcggg tcgtccgtac
      901 cgcatccagc accgcttgct cgagagcgtc gcctgggcga gcgtggcgag gacattgggg
      961 ttcgggaacg aactctcggc gggatagttt cgctccggga ggagcatgct cgaacggatc
     1021 gattcgatgc gctcggcgag atcgcgtggg aggacgcgct cgatcttcgt cagcgcctgc
     1081 gtgaccgggc ggggaagatc ggtcgatgcc gtcgtgcggg aggcgaggag ggcgatcgtc
     1141 agtccgatcg cttcctccgg gctgaacatc agtggtggca ggcggaagcc gggccgcagc
     1201 cagtagccac cggtgcgtcc cgccatcggc tcgacgggga tgcccatctc ctgaagtgtg
     1261 atgacgtagc ggcggatcgt ccgcgtcgtc acgccgagct cctcggcgag gacggggccg
     1321 ctgattcccg gtcgatcctg caggagttcg agtagcgcca gcacccggct ggcgggcttt
     1381 gtggcctcga tcatcatcgc cagacatccc tccgcgaagc acatttcgat gccccaagta
     1441 tgccagataa atgaggaccg aaagtgtccg catcgacctc tatgatgaat gcagatcagg
     1501 acgagatgga agcgacgagc gcgaacagac gcggagatgg aggacgagga tgacggaatc
     1561 cgtagcggag acgacatgga cgaatgagac ggcactgctc gtgatcgacg tgcagaaagg
     1621 cgtcgtcgtc gacggcttcg atcgggacgg ggtggtctcg cgcatcgccg gattgatcgg
     1681 ttacgcgcgt gcgcatcagg tgcccgtgat ctacatccag catgaggagc cgagctaccc
     1741 accgatggcg cgcggcggcg gtgactggga gatcgtggac gagatcgcgc cgaacgtcgg
     1801 cgagccggtg attgccaagc aatacccgga ttcgttcgcg gagaccgatc tggaatcgac
     1861 gctcgacgat ctcggcgtgc gtcatctcgt gatctgtggc gcgcagtcgg atgcctgcgt
     1921 gcgggccacg acctaccggg cgatgatcga aggatacgac gtcaccctcg ttggtgacgc
     1981 gcatacgaca tcggatcgac cgtactcggg tggaacgatc ccggcggagc aggtcatcaa
     2041 tcacgtcaac atcgcgacgc catacatcgc gtatccgggc cgggcgccgc gggtcgtgag
     2101 gcaggcggaa ctggtgaccg gagagccagt cacgatctga accgctcccc gcaatccgct
     2161 cgcctcgcat gcatccccga agcgttggtt cgaacagaga atacgggcgc gtagccctga
     2221 atgaggagtg agcgacgatg cttcctttca atcccgacat catgaacgtg gcgattgcgg
     2281 agcagcagga gcggattcgg atccatatgc tgcgggcgca ggcgagagga ttcgccgacg
     2341 atgtcaatct ccttcgccgt ttgatcggtc gtgcgctcgt ctggagcggt cgtcgtgtcg
     2401 gtggccccgt ggtggtggcc gccacgacgc cggaaagcct ggcgccggtc atcgatcttg
     2461 ggtcccatcg gctggaagca gatcgcaacc ttgaacgcgc cgcctgacgt cagggcggga
     2521 aggtgggcag catggagctg ctggtcttga tcgtggtact cgttctcctt ggtttcgcgg
     2581 cgttgtggtt cggtacggat tcgcgcgatg atgcgccgaa ctggccgtag cggacgacga
     2641 atcgactgac acacgcggac cgggccagca gtgcgctgac ccggtccttt ttcgtttcca
     2701 ggaggagaga tcgtggaacc cgtgagcaac ttcatctggc ggccgtggga agaacccggc
     2761 agcgagcatc tccgcatcca tcgcgctccg agcggcgaga tgatcgccga cagtcagctc
     2821 gtgacggtga tcgacggcgc cgtgctgcgg ctttcctacc gggtcgagct tgacgcgaac
     2881 tggcggacgc tttccgtgcg tgtcgcctgc gagcagccga acggaacggc gatcgggctc
     2941 gcgctgcagt cggatggtgc cgggaactgg agggatggca gcgggctcgc gctgccggaa
     3001 ctcgggggat gcctcgatat cgacatccag gcgacgccct tcacgaacac cttgccgatc
     3061 cgccggctga atctggcggc ggaggaatcc gaggtgatcc gcgtcgtcta catcccggtg
     3121 ccgacgctcg acgtcgcccc gatgaaacag cagtacaccg ggatcgacga tcggcacgtg
     3181 cggtacgaat cgatccggag cggtttcacc cgcgaactcg agatcgaccg cgatggattc
     3241 gtcgtgcagt atcccggtct tttccggcgg atctggccca tatgatcggg acatccggcc
     3301 aagggcgcgt ggtacacttt cgctccagaa acgaggtgtg ttcgcaacga acgtcgccca
     3361 cttcggcgcc atagccaagc ggcaaggcac gggtttgcaa aaccctgatc gtcggttcga
     3421 atccgactgg cgcctccacc acgatcatgc aagcatggag cgcgatgagg gacgtcagct
     3481 tggcgtccct cttatcatgt ctccatggcg tttgccatga gagacaccag acggaccggg
     3541 acgggaagat catgtcatct atcgaagagc aacccaccac gtccaccacc atcgatgaat
     3601 cggattcgcc cacggcgaca tccgcggcgc tgtcggaaga ggaacagcgc cggcgccgga
     3661 gccgcgcgat cgcctggggc gccggaatct ttctcgcgtt cggcctcgga ttgccgatct
     3721 ggtatgcgct cgacgactgg cgagcgggcc tgatcacggc gatcgttgtc ggcggcctca
     3781 ccgtcgtcgg ctacctgaag gcgggctcgg accacgccga gcagcgtgcc gagcgccagg
     3841 cgcggatgta cagccgtgag gatcaggacg acgaggacga cgacggcagc tacaactaga
     3901 ctgcctggcg ttcatcaggg gacgtaaacg acgtgagcga gcgggacgag cggttcgaga
     3961 cggatggcga tccagagccc gcctacatgg cgtggcaacg gggaccggtg cttcctcacg
     4021 aggtcacgac ggagacgcgg cgcggattcg acgggcacct gcttcatgtc cgtgtcgatg
     4081 acgtgatcct gccgtccggg cggatgtcgc ggcgggagat cgtcgagcat ccggggtcgg
     4141 tcgtgatcct gccgctgacc gtcgatcgcg aggtcctgct gatccgccag tggcgacatg
     4201 tgacgaaccg ctatcaactc gagttgccgg caggggtgat cgaccccggc gagttcttca
     4261 tgcagaccgc cggtcgtgaa ctgatcgagg agaccgggca cgaagccggg gacctgcgtc
     4321 atcttgcgac gatcttcatg agtcccggct acacgaacga gcgcaccgcc ttcgtgcttg
     4381 ccactggatg tcgcgcgatc gagcgcgagg cggatgtcga cgagccgatg gaattgattc
     4441 ggcgaccgct ttcgtcgatt gccgatctgc tgatgcccgg aaatgcggcg atcgaggacg
     4501 cgcagacggt tcttggtctc ctctggctgc tccgcc
//
LOCUS       DASTMD010000725         2646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3102248, whole genome shotgun sequence.
ACCESSION   DASTMD010000725 DASTMD010000000
VERSION     DASTMD010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3102248"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..961)
                     /locus_tag="VFQ54_10760"
     CDS             complement(35..961)
                     /locus_tag="VFQ54_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0165513.1"
                     /translation="MIEARRLTKKYGEKVAVNDLTFTIRPGQVTGFLGPNGAGKSTTM
                     RMILGLDRPTSGSVTVNGKPYIDQAAPLHEIGALLEARSVHTSRSAYNHLRAIAATAG
                     IPTWRVDEVIALVGLGGVSRKPAGSFSLGMGQRLGLASALLGDPETIMLDEPVNGLDP
                     EGVRWVRALLRDLADQGRTVMLSSHLMSEMELIADHILIIGRGQIILDTTMEDLRLSS
                     TTETVLVRTPDPDGLRRAIAAPGVTVSSSNQHSMEVAGMTAEQIGSLAADNRITLFEL
                     TPQKETLEDIFMKLTADAVEYHTESLAKGIAA"
     gene            1489..2355
                     /locus_tag="VFQ54_10765"
     CDS             1489..2355
                     /locus_tag="VFQ54_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0165514.1"
                     /translation="MPIARYLTADSVIPRLGVLDGETIYEFRHTNGPETLDAALTMLV
                     AELHAAVQNALDNTRQTLNILEVSLLPPIDSQEVWASGVTYSRSRDARMEESTQKDVY
                     DLVYEAERPELFFKATPSRVSGPEGTVAIRGDSTWDVPEPELAIVINSSQEIVGYTIG
                     NDVSSRSIEGENPLYLPQAKVYSGCAGLGPVIVLPDELGDPTDLRISLVIERNGAVNF
                     EGSTSTDQIHRKLPELVDYLHRYNAFPSGAILMTGTGIIPPAEFTLEDGDVVSITIDK
                     IGTLRNPVIRLA"
BASE COUNT          501 a          798 c          810 g          537 t
ORIGIN      
        1 ggcgcatcag atacttcgtg aatcgaaacg gaactcatgc tgcaattccc ttggcgaggc
       61 tctctgtgtg atattccacg gcatcggcag tcagcttcat gaagatgtcc tcgagggttt
      121 ctttttgcgg ggtcaattca aagagggtga tgcgattgtc tgcggcaagc gagccgatct
      181 gttcggcggt cattccagca acctccatcg agtgctgatt cgatgagctt acggtgactc
      241 ctggtgcggc gatggcccga cgcagaccat ccggatcggg cgtacgcacg agcacggtct
      301 cggtggtgga tgagaggcgg aggtcttcca tggtggtgtc caaaataatc tgccctcgcc
      361 cgatgatcag gatgtgatcg gcgatcaact ccatctcgct catcaagtgg ctggagagca
      421 tgacggttcg gccctggtct gccaggtcac gaagcagcgc tcgaacccag cggacgcctt
      481 ccgggtcaag tccattgacc ggctcgtcga gcatgatggt ctcggggtca ccgagcagtg
      541 ccgatgccag gcctagtcgc tgacccatac cgagcgagaa tgatccggcc ggtttgcgac
      601 tgacgccacc gagaccgaca agtgcaatga cctcatccac gcgccaggtg ggaataccgg
      661 ccgtggcggc tatcgcccgc agatggttgt aggcagagcg cgaggtgtgg accgatcgcg
      721 cttccagaag cgctcctatt tcatggagtg gtgcggcttg gtcgatgtag ggcttcccgt
      781 tgacggtcac tgagccggat gtgggacggt ccagccccaa aatcatgcgc atggttgtgg
      841 acttgccagc tccgtttggc ccgaggaatc cggtcacctg gcccgggcga atggtaaagg
      901 tgagatcgtt gaccgccacc ttctcaccgt atttttttgt cagtcgacgt gcttctatca
      961 tgtgttcttc tcctagctct gcgcattcgg gattcaatcc cttagcacca actctaggaa
     1021 gaaagcgacg gtatgcacat tcaccgatag gtggaaattc gcggtccaca tacgacttgc
     1081 gatgtatatc gcctcgacgg gtatcgctgt ccgatttgtg gaaatagtgg caaagcgtac
     1141 gcgagattat ggaggagagg gagcgcacga gaatccagat agtgttacat gctcctctct
     1201 gttgtagaca ggaacgatcc catccttatg cctgattttc gtcaggcaaa ggactgttac
     1261 cctcgactag tcacggtctt ctactcgggt actccaggca aagaatcgag ggagcggtca
     1321 cgagattgcg cgtcctccgc gaaatgttgg aagggcgggg gtcgtgtccg ttctcgaagt
     1381 gcataacagg cacgagcgtc ggcgcccgcg ccacgatctc gggtaggatt cggtcagctc
     1441 tctcccatcc ggagaatcga catccgccgc atacggaggt tcacgctcgt gcctatcgcc
     1501 cgctacctca ccgccgacag cgtcataccg cgcctcgggg ttcttgatgg tgaaaccatc
     1561 tacgagttcc gtcacacgaa cggcccggag acgctcgatg cggccctgac catgctcgtt
     1621 gccgagcttc acgcggcggt ccagaacgcc ctcgacaaca cgcgccagac gctgaacatc
     1681 ctcgaagtca gcctcttgcc gccgatcgat agccaggaag tctgggcctc cggcgtcacg
     1741 tactcccgta gccgcgacgc ccgcatggag gaatcgacgc agaaggatgt ctacgatctc
     1801 gtctacgagg ccgagcgacc ggaactcttc ttcaaggcga cgccgagccg tgtctccgga
     1861 ccggaaggaa ccgtcgccat tcgcggcgat tcgacctggg acgtgccgga accggaactc
     1921 gcgatcgtca tcaacagcag ccaggagatc gtcggctaca cgatcggcaa cgacgtctcc
     1981 tcccgctcga tcgagggtga gaatccgctc tatcttcccc aggcgaaggt ctacagcggg
     2041 tgcgcggggc tgggtccggt gatcgtgctc cctgacgagc ttggcgatcc gaccgacctc
     2101 aggatctcgc tcgtcatcga gcgcaacggc gcggtcaact tcgaggggtc gacctcgacg
     2161 gaccagatcc atcgcaagct cccggaactc gtcgactatc tccatcgcta caacgcgttt
     2221 ccgagcggcg cgatcctgat gacggggacg gggatcatcc cgcccgccga gttcacgctc
     2281 gaggatggcg atgtcgtcag catcacgatc gacaagatcg gcaccttgcg caatcccgtg
     2341 atccggctcg cataaaccga gctgggcgga gagcgggtcg cgggcggcgc tccttggcgc
     2401 ggggtgtgtt tctgccgaat cagcgctacg gggcgcgagt cacggacggc gctccttggc
     2461 gtaaggggtg tttctccgga atccgctttg cggcggggta tgaaaccccg cccttgtatg
     2521 tttggggggt ggcagcgtcc cccggccccg cccatggtct cggacgaccg tttggtagtt
     2581 ccggggacgc agcccgcctg ctggcgacgc ctcgcctccc cgcgaacggt tacctgggta
     2641 tccccc
//
LOCUS       DASTMD010000726         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2123566, whole genome shotgun sequence.
ACCESSION   DASTMD010000726 DASTMD010000000
VERSION     DASTMD010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123566"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VFQ54_10770"
     CDS             complement(<1..786)
                     /locus_tag="VFQ54_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU0165515.1"
                     /translation="MEYIDLHAHMISRTTDDYEKMALSGCVAVSEPAFWAGYDRRSAD
                     VFADYFDHITDFEPKRAAQYGIQHYAWLCLNPKEGEDRELTKQVLQIIPEFLERPTVL
                     GIGEIGLNRVTRNELETFKDHVSLALEHNQLIHIHTPHLEDKYKGTKVIVETLAADGR
                     IDPSRVMVDHAEEHTMPIILDAGFWTGVTLYPITKTTPGRAVDMFERYGTERICVASA
                     CDWGPSEPVAVPHFALEMRRRGYGKDLIQKIIYDNPAQFLGQSP"
     gene            complement(801..1100)
                     /locus_tag="VFQ54_10775"
     CDS             complement(801..1100)
                     /locus_tag="VFQ54_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165516.1"
                     /translation="MAANDINSCPLSQQQLIDEFFMESRTKILDIAAFLDRLDRSVSE
                     DGRNDFRIVAMKHAMEELNSQVPGRIERVLMIFSDQNTELLDELDRKAAYGASIR"
     gene            complement(1048..>2019)
                     /locus_tag="VFQ54_10780"
     CDS             complement(1048..>2019)
                     /locus_tag="VFQ54_10780"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF004852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU0165517.1"
                     /translation="AIKNDHSSVDTIQRAINDHGIDRHSYVIAVGGGALLDMVGYAAA
                     TAHRGVRLIRIPTTVLSQDDSGVGVKNSVNAFGKKNFLGTFAPPFAVIDDMALLLRLP
                     DREWLGGIAEAVKVSLLKDASFFHYIEEHAGDLVARDAETMRWLIYRCAQLHLQHIAT
                     SGDPFEFGSSRPLDFGHWSAHKLEQISNYRWRHGEAVAIGISLDSTYAWLNGMLPEAD
                     WRRIVNVFLALKLPIFAPELTASAEGDPPTVLAGLNEFREHLGGELTIMLLHGIGDGF
                     EVHEMDEQLIVRSIEVLRRLQDEHESKEDANGRERHQQLPTLATTAD"
BASE COUNT          375 a          603 c          625 g          416 t
ORIGIN      
        1 cggcgactgt cccaggaact gtgccgggtt gtcgtagatg atcttctgga tcagatcctt
       61 gccataccca cgacggcgca tttccagcgc gaagtgcggc acggcaaccg gctcgcttgg
      121 gccccagtca caggcagatg cgacgcagat tcgctcggtg ccgtagcgct cgaacatgtc
      181 gaccgcacgg cccggggtgg tcttggtaat cgggtagagc gtgacaccag tccagaagcc
      241 ggcgtcgagg atgatcggca tcgtgtgctc ttcggcgtga tcgaccatca cgcgcgaggg
      301 atcgatgcgg ccgtccgcag cgagtgtctc gacgatgacc ttcgtgccct tgtacttgtc
      361 ctcgaggtgc ggggtgtgga tgtggatcag ctggttgtgc tcgagggcga ggctgacgtg
      421 atccttgaag gtctcgagtt cgttgcgggt gacgcggttc aggccgatct ccccgatgcc
      481 cagcaccgtc gggcgctcga ggaactcggg aatgatctga aggacctgct tcgtcagctc
      541 ccgatcctcc ccttccttcg gattgagaca gagccaggcg tagtgctgga ttccgtattg
      601 cgcggcgcgc ttcggctcga agtcggtgat gtgatcgaaa tagtcggcga agacatccgc
      661 cgaacgacgg tcgtatccag cccagaaggc tggctccgag accgcaacac atcccgagag
      721 cgccatcttc tcgtagtcat cggtggtacg ggagatcatg tgcgcatgaa gatcgatgta
      781 ttccatggga cggctccttc ctagcgaatc gacgcaccgt aggcggcctt gcgatcgagt
      841 tcatcaagga gctcggtgtt ctggtcgctg aagatcatga ggacgcgttc gatgcgcccc
      901 ggaacctggg agttgagttc ctccatcgcg tgcttcatcg ccacgatccg gaagtcattc
      961 cgaccatctt ccgaaaccga gcgatcgagg cgatcgagga atgcggcgat gtcgaggatc
     1021 ttggtcctcg actccatgaa gaattcgtca atcagctgtt gttgcgagag tgggcagctg
     1081 ttgatgtcgt tcgcggccat tcgcgtcctc cttggactca tgttcgtcct gcaatcttcg
     1141 caggacctcg atacttcgga cgatcaattg ctcgtccatc tcatgcactt cgaagccgtc
     1201 accgatcccg tggagcagca tgatcgtcag ttctccaccg agatgttccc ggaactcgtt
     1261 gagtcccgcg agcaccgtgg gagggtcacc ctccgcggat gcggtcagtt ccggcgcgaa
     1321 aatcggcagt ttcaatgcga ggaagacgtt gacgatccgc cgccaatccg cctccggcag
     1381 catgccgttg agccaggcgt aggtcgaatc cagcgagatg ccgatcgcca cggcctcgcc
     1441 atgtcgccac cgatagttcg agatctgctc cagcttgtgc gccgaccagt gaccgaagtc
     1501 gagggggcgc gaggatccga attcgaacgg gtccccgctc gtcgcgatgt gctggagatg
     1561 cagctgcgcg catcgataga tgagccagcg catggtctcc gcatcgcgcg cgaccaggtc
     1621 gccggcgtgc tcctcgatat agtggaagaa ggacgcgtct ttcaggaggg agaccttgac
     1681 ggcctcggcg attcctccaa gccactcccg gtccggcagc cggagcaaca gtgccatgtc
     1741 atcgatcacg gcaaatggcg gcgcgaacgt tcccagaaag ttcttcttcc cgaacgcgtt
     1801 cacgctgttc ttcacgccga cgcccgagtc atcctgggat agtacggttg tcggaatccg
     1861 gatcaggcgg acaccgcgat gcgccgtcgc ggcggcgtag ccaaccatgt ccagcaaggc
     1921 accgccgccg accgcgatga cgtacgagtg ccgatctata ccgtgatcgt tgattgcccg
     1981 ttggatcgta tcgaccgacg aatgatcgtt cttgatcgc
//
LOCUS       DASTMD010000727         4102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1763725, whole genome shotgun sequence.
ACCESSION   DASTMD010000727 DASTMD010000000
VERSION     DASTMD010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763725"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFQ54_10785"
     CDS             <1..195
                     /locus_tag="VFQ54_10785"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEU0165518.1"
                     /translation="LALSSEYLAEARHHMPRLPDEASVTAEAHDRVVEALLQRDPDAS
                     AAAMREHLRSVEEGLGIFLP"
     gene            289..1953
                     /locus_tag="VFQ54_10790"
     CDS             289..1953
                     /locus_tag="VFQ54_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0165519.1"
                     /translation="MNEEPTHVLDEIIDSYRSGGMSRRELLAKATIAAAGIALTSRLT
                     ANSTLAQGTPKSGGTFTYALNQTGDTLDPAVTTYAVTNKININVFDPLIWQASDLSFV
                     PGLAESWEVAPDSTSFTFKLRKDVKFHDGTPLNADAVKFSWDRIMDPATQSKTAIGQM
                     GPFKQAVVVDDYTIRAEFSEPFAPFLDAVSQSYCSPVSPTAVKKAGTDFTHSLVGTGP
                     YKLKEYVENDHVTLERYDDYNWAPTIFKHQGPGYLDQIVFKMITDDSVRTGTLQSGET
                     DGIDSVPAKDVASFKADPDTYTLLNASAPGIPNLFTLNTEKAPTNDIAVREALNYGTD
                     KQTICTTLFFGQNTPAYGPMSAVTLGYDASIESMYPFDKDKAKSVLDAAGWVVADKGI
                     RAKDGVALKISLYTDTSDQTPPLLQAMWKEIGIDTEINQMDYNALIPIVTKGDANLGN
                     IGWIQGDPDVVRIILYSKNIESGYGWTRFKSAELDSLIVAAASTVDQAKRKDLYAQIQ
                     QLTMKNALVLPIDNLSAIYALKSDVKDFSVDSRGWYPWLYNVWLDR"
     gene            2047..2970
                     /locus_tag="VFQ54_10795"
     CDS             2047..2970
                     /locus_tag="VFQ54_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165520.1"
                     /translation="MVTYIARRLLIAVPVIWGVATIVFLIMRVVPGDPAQLMLSSSGG
                     NADQIATLRKSLGLDESIPQQYVEFMGHILQGNLGNSLRTNQPVTREIGNQLMSTLQL
                     TGFSMLLATVIGLVLGMVSAFWQRTWIDSLTMSLAMLGVCTPSFWLGLMLVSIFSFTL
                     HWFPATGTGGIDRLILPSITLALGSAAVIARLVRSSMIEALSQEYVVVARAKGMSELK
                     VVLSHVTRNALIPAITLIGLEIGSLLGGAVVVESVFSRQGIGRLAVNAIVSKDYTIVQ
                     GVVLFVATAFVLVNIIVDLAYGFLDPRIRHS"
     gene            3011..3928
                     /locus_tag="VFQ54_10800"
     CDS             3011..3928
                     /locus_tag="VFQ54_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165521.1"
                     /translation="MSSQVTTAERPSAANSLAQLAPTRTPSRGRSLLRQYARNRGAVV
                     GAVIVLIIIAAALLQQWINPYDPLGVSPLNALKSPTWRHPMGTDEYGRDVFSRVMDGA
                     HVSLEIGVFAVVISTLVGSSLGLVSGFKEGVIDGVIMRLVDMLLAFPGVLLALSIIAV
                     LGANLRNVVIAVGIGTIPVYARVVRGSVLAVKNSTYIDAARVSGCSEIRIAIVHIVPN
                     ILASILVLATIGVAYSILNGAALSYLGLGVQPPSAEWGLMVSDGRSYLRDAWWMATGP
                     GLALAILVLSINLVGDGVRDATDPRSRAR"
BASE COUNT          732 a         1410 c         1203 g          757 t
ORIGIN      
        1 ctcgccctga gcagcgaata cctcgctgaa gcgcgccatc acatgccacg actccccgat
       61 gaagcctcgg taaccgcgga agcccacgat cgcgtggtag aggcactgct ccagcgggac
      121 cccgacgcgt ccgccgcggc aatgcgggaa cacctgcgat cggtcgagga aggactcggc
      181 atcttcctgc cctgaattcc ccggcaacgg attcacgctc gaccgttctc agttctctcc
      241 gggtccccgt ctcgtgctgc cccataaacg aatgaggttg accgttccat gaacgaagag
      301 cccacgcacg tcctcgacga gatcatcgac agttaccgat cgggcggcat gtctcgccgc
      361 gagttgctcg caaaagcgac gatcgccgca gcgggaatcg ccctcaccag ccggctcacg
      421 gcgaactcca cgctcgccca gggcacgccg aagtccggtg ggacgttcac ctacgccctg
      481 aaccagacgg gggacacgct cgaccccgcg gtcacgacct atgcggttac gaacaagatc
      541 aacatcaatg tcttcgaccc gctgatctgg caggcgtccg atctctcgtt cgttccgggc
      601 ctcgccgagt cctgggaggt cgcgccggac agcacgtcat tcacgttcaa gctacgtaag
      661 gacgtgaagt tccacgacgg cacgccgttg aacgccgatg cggtcaagtt ctcgtgggac
      721 cggatcatgg atcccgccac gcagtcgaag accgcgatcg gccagatggg tccgttcaag
      781 caggcggtcg tcgtcgacga ctacacgatc cgcgccgagt tctcggaacc cttcgcgccg
      841 ttcctcgacg cggtgagcca gtcgtactgc tcgccggtct cgccgaccgc agtcaagaag
      901 gccggtacgg actttacgca cagcctcgtc ggcaccggcc cgtacaagct caaggaatac
      961 gtggaaaacg accacgtcac gctcgagcgg tacgacgact acaactgggc gccgacgatc
     1021 ttcaagcacc agggaccagg ctatctcgac cagatcgtct tcaagatgat caccgacgat
     1081 tcggtccgca ccggcaccct gcagagcggc gagaccgatg gcatcgattc cgtcccggcg
     1141 aaggacgtcg cctcgttcaa ggccgatccc gatacctaca ccctgttgaa cgcgtcagct
     1201 ccgggcatcc cgaacctctt cacgctcaat acggaaaagg cgccgacgaa cgacatcgcc
     1261 gttcgcgagg cactcaacta cggaaccgac aagcagacca tctgcaccac gctcttcttc
     1321 ggccagaaca ccccggctta cggaccgatg tctgccgtga cactcggcta cgatgcctcg
     1381 atcgaatcga tgtatccgtt cgacaaggac aaggcgaagt cggtgctcga cgcggcgggt
     1441 tgggtcgtcg ccgacaaggg gattcgcgcc aaggacggcg tcgccctcaa gatctccctc
     1501 tacaccgaca cctccgacca gacgccgcca ctgctccagg cgatgtggaa ggaaatcggg
     1561 atcgataccg agatcaacca gatggactac aacgccctga tcccgatcgt caccaagggt
     1621 gacgccaacc tcggcaacat cggctggatc cagggcgatc cggatgtcgt ccggatcatc
     1681 ctctattcca agaacatcga gtccggctac ggctggaccc gcttcaagag cgcggagctt
     1741 gactcgctca tcgtcgcggc ggcgtccacc gtcgatcagg cgaagcgcaa ggatctctac
     1801 gcccagattc agcagctgac gatgaagaac gcgctcgtgt tgccgatcga caacctctcc
     1861 gcgatctatg ccctcaagag cgacgtcaag gacttcagcg tcgacagccg cggctggtac
     1921 ccgtggctct acaacgtctg gctcgatcgc tagcaaccgg ttaccccgtt cgggacgagg
     1981 gatgcgcggc tgggcgcatc cctcgtcggg gcggacgctc gctttctcct cacccggagt
     2041 tcgatcatgg ttacctacat cgcccgccgg ttgctgatcg cggttcccgt catctggggc
     2101 gtcgcgacga tcgtctttct catcatgcgc gttgtccccg gtgacccggc gcagctgatg
     2161 ctctcgtcgt ccggcggcaa cgccgatcag atcgcgacgc tgcgcaagag tctcggtctc
     2221 gacgagtcga tcccccagca gtacgtggag ttcatggggc acatcctgca ggggaacctc
     2281 gggaactcgc tccgcacgaa ccagccggtc acccgggaga tcggcaatca gctgatgagc
     2341 acgttgcagc tcaccggctt cagcatgctc ctggcgacgg tgatcggact cgtccttggg
     2401 atggtttccg ccttctggca gcgaacgtgg atcgactcgc tgacgatgag cctcgcgatg
     2461 ctcggcgtct gcacgcccag tttctggctc ggcctgatgc tggtctcgat cttctcgttc
     2521 acgctgcact ggttcccggc gacgggcacc ggcgggatcg accggctgat cctcccgtcg
     2581 atcacactcg ccctcggttc ggcggcggtg atcgcccgac tcgtccgctc gtcgatgatc
     2641 gaggccctga gccaggagta cgtcgtcgtc gcccgcgcga aggggatgtc cgagctcaag
     2701 gtcgtgctca gccacgtcac ccgcaacgcc ctgattccgg cgatcactct gatcgggctc
     2761 gagatcggat cgttgctcgg cggcgccgtc gtcgtcgagt cggtcttctc gcgacagggc
     2821 atcggcagac tggcggtgaa tgcgatcgtc tcgaaggact acacgatcgt gcagggcgtc
     2881 gtgctcttcg tggcgaccgc cttcgtgctc gtcaacatta ttgtcgatct cgcctacggt
     2941 ttcctcgatc ctcgcattcg ccacagctag cgcggccccc ggccttcgac ttcgcccagg
     3001 aagatcgccc atgagttccc aggtcacaac cgccgagcgc ccttcagcgg caaacagtct
     3061 cgcccaactc gcgccgacgc gaactccttc acgtggccgt tcgctgctcc gtcagtacgc
     3121 gcgaaaccgg ggagcggtgg tcggcgcggt catcgtcctg atcatcatcg cggcggcgct
     3181 gctccagcag tggatcaacc cgtatgaccc attgggcgtc agtccactca acgcgttgaa
     3241 gagtccgacg tggcgtcatc cgatgggcac cgatgaatac ggccgcgatg tcttctcccg
     3301 ggtgatggac ggcgcccacg tctcgctcga gatcggcgtc ttcgccgttg tcatctcgac
     3361 gctcgtgggt tcctcactcg gcctggtctc cggcttcaag gaaggcgtga tcgacggcgt
     3421 gatcatgcgc ctggtcgaca tgctcctcgc gttccccggt gttctgctcg ccctctcgat
     3481 catcgccgtg cttggagcga acctgcggaa tgtcgtgatc gcggtcggca tcggcacgat
     3541 tccggtctac gcccgcgtgg tgagaggctc ggtcctcgcg gtgaagaact cgacttatat
     3601 cgacgcggcc cgcgtatccg ggtgctcgga gatcaggatc gcgatcgtcc acatcgttcc
     3661 caacatcctc gcctcgatcc tcgtgcttgc gaccatcggc gtcgcctact cgatcctgaa
     3721 cggcgccgcg ctctcctacc ttggactggg tgtccagcca ccgagcgcag aatggggtct
     3781 gatggtgtcc gacggtcgat cctatcttcg cgacgcctgg tggatggcga cggggccagg
     3841 ccttgccctc gcgatcctcg tcctgtcgat caatctcgtc ggcgacggcg tccgcgacgc
     3901 aaccgacccc cgctcccgcg cccggtaggc cggaaacgac ccgtctggcc cgtggaccgg
     3961 atcgttgccc gagcgacgct cacgcctccc gatcacgacg atcccgtggc gggccacccc
     4021 gtgtggccca tcgaccgcag gtcgacatga ccgtcgcagg agcaacactc acccctcccg
     4081 atcgttggat gaacagtaga cg
//
LOCUS       DASTMD010000728         3813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_604869, whole genome shotgun sequence.
ACCESSION   DASTMD010000728 DASTMD010000000
VERSION     DASTMD010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604869"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFQ54_10805"
     CDS             complement(<1..401)
                     /locus_tag="VFQ54_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165522.1"
                     /translation="MTPEDQISQEQGDVAVVEAPVRRKVGRPRRKVVEETATTEAAVE
                     QTAAPTTVPETAPAAAVEAAEPAVAPVEQPVPTAPTETPAEPVVEAAVSESDTAATEA
                     SSASDPSAPSGEARPHQRTGRPERERRVTPGS"
     gene            complement(878..1870)
                     /gene="glpX"
                     /locus_tag="VFQ54_10810"
     CDS             complement(878..1870)
                     /gene="glpX"
                     /locus_tag="VFQ54_10810"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEU0165523.1"
                     /translation="MSTNPIIGRNLALELARVTEAAALMAGRWMGRGMKEASDQAAVD
                     AMREVLNTIEMEGIIVIGEGEKDEAPMLYDGETVGRAEYPKVDIAVDPIDGTRLLAEG
                     MPNSVCVLAIAERGTMYKWQHIAYMEKIAVGPDAVGAIDIDLPVATNLANVAARKGVD
                     IAALTVVILDRPRHRQLIEDVRAAGARIKLISDGDVAGALMTAMSGTGIDVLMGIGGS
                     PEAVIAACALKCTGGDMQTRLWARNDEERELAAREGIDLTKVFTLNDLVGGNDVFFAA
                     TGITDGELLDGVKYLPEGARTHTLVMREFSGTVREIRSLHPLSKLKSLGTPTLE"
     gene            complement(1908..>3813)
                     /locus_tag="VFQ54_10815"
     CDS             complement(1908..>3813)
                     /locus_tag="VFQ54_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEU0165524.1"
                     /translation="QDQLYRKDLPDVRTALVETGVTDELRVAVMKGANNYICLTQWFK
                     HMRTAPADAYDASLRAKVLLWLGQTQTGDRAELRLTQEEENYWRPIAAGDQVCTMKSC
                     AYARRNQCFLPRARNQAMNAHIVIANHALLLAGGSPEGHVMGSFNRLILDEAHHLEDE
                     ATRAFGFYLDRRGPEEQVNSLIRSQGASLEGAFAIAATFLTRLPQPVAVEAAPKALER
                     VSAATGSASKAIMLLGELFSRVGEFLPPPKRTSQSYADSLRITDSVRRRGEWIELELI
                     WQELDGNLRQILGTGDWFLQTLDKINLPDDLEHPETRARDQITLDLSRSLVELNTHVQ
                     QMTSIFGQPSRMEVCWIRRSVQAGSISLNVAPLMVDVLLQERLYASVRTVVLTSATLT
                     IDGSFNYMASRLGLEEAEGLALGTPFDHEKSTLLFVTDDMPEPNDGRYQHELNQTLIE
                     LLAATEGRALVLFTSYSALQATHRAIKQPLEQHGVVVLGQRIDGSARQLIERLRSTPG
                     TVVLGTSTFWEGVDIVGDALSLLVITKLPFPVPSEPVIEARSELLDNPFLDYSVPHAV
                     LKFKQGFGRLIRSATDHGVCAVLDRRVVSKRYGSSFVQSLPPARVQIGSVHDLPYSAA
                     RWLRARPGAE"
BASE COUNT          662 a         1246 c         1176 g          729 t
ORIGIN      
        1 gaaccgggcg tcacgcgtcg ctcgcgttcc ggacgcccgg ttcgctgatg cggacgcgcc
       61 tctcccgagg gagcagacgg gtcggatgcc gaagaggcct ccgttgcggc cgtgtcggac
      121 tcggaaactg cagcctcgac gacgggttcc gcgggtgtct ctgtgggcgc ggtcggcaca
      181 ggctgctcta ccggggcaac agcgggctct gcggcttcga cagccgccgc aggagccgtt
      241 tcggggacag ttgtgggtgc ggcggtctgc tcgacggcgg cctcggtggt tgcggtctct
      301 tccacgacct tgcggcgggg acgacctacc ttgcggcgaa ccggggcctc gacgacggcg
      361 acgtcgcctt gttcctggga gatctgatct tcaggggtca aaagaatacc tccggacctg
      421 agttggtact ggtaatgatg aaatcggtca aacggacgcg aggcgccgtt cagcttgagc
      481 tatggttttg aatgtgctga ttttgagaca cgatggttgt cggttctctt tatgtgcttg
      541 aggtaccgcg ttcccgcagg cgaagaagca tacagcgatg ctcaccgtgt gacggcttat
      601 gcccggatcg taccgtcgac ccgacatcct ctatctcatc gaggaaccga tcttcgcgtt
      661 agggagtgca gggaggattg tctcgccatc ggacgaactg gtcccgtcat ggcgtcgcag
      721 accagcgcgt tcacatactt tctgctgatc gagcgaagcg taccaccacc acgcggatgc
      781 gtcaacagat ggccgcgctt cgcccgcatt ttcgaggtat tccagtattt tccggctgga
      841 ttcggtcgaa ttccggagcc caaccgggac cgatcgctta ctcgagcgtc ggagttccaa
      901 gcgatttcag cttgctgagc ggatgaaggg atcggatttc acgtactgtt cccgagaact
      961 cgcgcatcac cagcgtgtgc gtgcgtgcac cctccggcag gtacttcaca ccgtccagca
     1021 attcgccgtc ggtaatcccc gtcgccgcga agaagacatc gtttccgccg accaggtcgt
     1081 tcagcgtgaa gaccttcgtg aggtcgatcc cctcacgcgc cgccagttcg cgctcttcgt
     1141 cattgcgcgc ccagagacgc gtctgcatgt cgccccccgt gcacttgagc gcacacgccg
     1201 cgatcaccgc ttccggcgaa ccaccgatgc ccatcaggac gtcgattccg gtgccggaca
     1261 tcgcggtcat cagggcgccg gcgacgtcac catcggagat cagcttgatc cgcgcaccag
     1321 cggcccgcac gtcctcgatc agctgcctgt gacgcggccg atcgaggatc acgacggtca
     1381 acgccgcgat atcgacgccc ttcctggcgg ccacattggc cagattcgtc gcgacgggca
     1441 gatcgatgtc gatcgccccc acggcgtccg gaccgacggc gatcttctcc atgtaggcga
     1501 tgtgctgcca cttgtacatc gtgccgcgct ccgcgatcgc gagcacgcag acggaattcg
     1561 gcatcccctc ggcgagaaga cgcgtcccgt cgatcggatc gacggcaatg tcgaccttcg
     1621 ggtactcggc acgcccgacg gtctcgccgt cgtagagcat gggcgcctcg tccttctccc
     1681 cctcgccgat cacgatgatg ccttccatct cgatcgtgtt cagcacctcg cgcatcgcat
     1741 cgacagcggc ctggtcggac gcttccttca tgccgcgacc catccagcgg cccgccatca
     1801 gcgcggccgc ctcggtgacg cgcgcaagct caagcgcgag attccttccg atgatcgggt
     1861 tcgtgctcac agattccatc cttcgattca ttcaagcatt cgtcgcgtta ttcggccccg
     1921 ggcctggctc gcaaccaccg ggcagcactg tacgggagat cgtgcaccga gccaatctgc
     1981 accctcgccg gcggcaagga ctgaacgaac gaagatccgt agcgtttgga aacgacacgc
     2041 cgatccagta cggcgcacac gccgtggtcg gttgccgagc ggatgagccg tccgaagccc
     2101 tgcttgaact tcagcacggc atgcgggacc gagtaatcaa ggaacggatt gtcgagcagt
     2161 tcacttcgcg cttcgatcac cggttcactc ggaaccggga acgggagctt ggtgatcacg
     2221 agcaggctca acgcgtcacc gacgatatca acgccttccc agaaggtcga ggtcccgagc
     2281 accaccgttc ccggcgtgct gcgcaatcgc tcgatcagct ggcgagcact gccgtcgatc
     2341 cgctgcccga gaacgaccac gccgtgttgc tcgagcggtt gcttgatcgc acggtgcgtc
     2401 gcttgcaacg cggaataact ggtgaagagc accagtgcgc gaccttccgt cgcggcgagg
     2461 agctcgatca acgtctggtt gagttcgtgc tggtatcttc cgtcgttcgg ctccggcatg
     2521 tcatcggtca cgaagagcaa cgtcgacttc tcgtggtcga acggcgtccc gagcgcgagc
     2581 ccttccgcct cctcgagtcc gagccggctc gccatgtagt tgaacgagcc atcgatcgtc
     2641 agcgtcgccg aggtcaggac caccgtccgc accgacgcat agagtcgctc ctgaagcagg
     2701 acatcgacca tcagcggcgc gacgttcagc gagatgcttc ccgcctgcac cgagcggcga
     2761 atccagcaga cttccattcg gctcggctgt ccgaagatgc tcgtcatctg ctggacgtgg
     2821 gtgttgagct cgacgagaga ccggctgaga tcgagggtga tctggtcccg cgcgcgcgtc
     2881 tccggatgct ccagatcgtc gggcaggttg atcttgtcga gcgtctggag gaaccagtca
     2941 ccggtgccca ggatctgccg gagattgcca tcgagttcct gccagatcag ttccagctcg
     3001 atccattcgc cgcgccggcg aacggagtcc gtgattcgca gcgagtcggc atagctctgc
     3061 gatgtccgct tcggcggcgg caggaattcc ccgacgcgtg agaaaagctc tcccagcagc
     3121 atgatcgcct tcgaggcgct ccccgtcgcg gcggagaccc gttccagcgc ctttggcgcg
     3181 gcctcaacgg caactggctg cggcaatcgc gtgagaaacg tcgccgcgat cgcgaacgcg
     3241 ccctcgagtg atgccccctg cgaccggatc agggaattga cctgctcctc cggtccccgg
     3301 cgatcgagat agaacccgaa tgcccgcgtc gcctcatcct ccagatggtg cgcctcgtcg
     3361 aggatcagcc ggttgaacga gcccatcacg tggccctccg gcgaaccacc agcgagaagc
     3421 agcgcgtgat tggcgatcac gatgtgggcg ttcatcgcct ggttgcgcgc gcgcggcagg
     3481 aagcactgat tacggcgcgc ataggcgcag ctcttcatcg tgcagacctg atcgccggca
     3541 gcgatcggtc gccagtagtt ctcctcttcc tgcgtcagcc gcagctcggc gcgatcgccg
     3601 gtctgcgtct gtccgagcca gagcaggacc ttcgcccgga gagatgcatc gtaggcgtcg
     3661 gccggggcgg tccgcatgtg cttgaaccac tgcgtgaggc agatgtagtt gtttgccccc
     3721 ttcatcaccg cgacgcgcag ctcatcggtg acgcccgtct cgaccagcgc ggtacggaca
     3781 tcaggaaggt ccttgcgata gagctgatcc tgg
//
LOCUS       DASTMD010000729         1802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2746434, whole genome shotgun sequence.
ACCESSION   DASTMD010000729 DASTMD010000000
VERSION     DASTMD010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2746434"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /gene="apbC"
                     /locus_tag="VFQ54_10820"
     CDS             <1..1083
                     /gene="apbC"
                     /locus_tag="VFQ54_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEU0165525.1"
                     /translation="LTQERILSALEPVLEPATGRSLIELKLVSDVAIDGGTVRFTIDL
                     LSPITSNRERIDRDVRAALAELPDVRSVVIDWRNKVRASGSGRSDAQPIDGVKNTIAV
                     ASGKGGVGKSTVAVNLAVSLARAGASVGLLDADVYGPSIPLMMGIHEKPLMKDNKIVP
                     LEAHGVKLMSIGFILDSQKALIWRGPLVAQLINQFLNDVHWGDLDYLVVDLPPGTGDV
                     QLTLVQKIPIAGAVIVTTPQEVALADALKGLKMFQEVKTPILGIIENMSWFISPETGT
                     RFPIFGEGGGQRTAEENGVELLGQIPIEMGIREGGDTGAPMASTHPESETGQAFAHLA
                     ERVANQLAVQAMAKPRKPTIMLRTVR"
     gene            1180..>1802
                     /locus_tag="VFQ54_10825"
     CDS             1180..>1802
                     /locus_tag="VFQ54_10825"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU0165526.1"
                     /translation="MNLSIEHLLAAPPDEGTLRHVYEALLDAAEDDIFLLDPECRVIY
                     VNAPALRAIRSALGADFTREAIVGKTLEELNYPESLVRQLAEHVGRAVGGETVDAEIP
                     YPAGVNVERLYEYRLSPLDEQIPAPLVIGIARDIESQKVDGRSRLALYEGERLARAEA
                     EAAVRIRDEFLSAAAHDLRTPLTAAQGRAQLMLRQLQRQGGLDPDRLR"
BASE COUNT          317 a          564 c          608 g          313 t
ORIGIN      
        1 ctcacgcagg aacgcatcct ctccgcgttg gagccggtgc tcgagccggc gaccggtcgc
       61 agcctgatcg agctgaagct cgtctccgac gtcgcgatcg acggcgggac ggtgcggttc
      121 acgatcgact tgctgagccc gatcacgagc aatcgtgagc gcatcgatcg cgatgtccgg
      181 gcggcgctcg ccgaattgcc ggatgtgcgg agtgtggtca tcgactggcg gaacaaggtg
      241 cgcgcgagtg gaagtggccg ctccgacgcc cagccgatcg atggcgtgaa gaacacgatc
      301 gcggtcgcct ccggcaaggg tggcgtgggc aagtcgacgg tcgcggtcaa tctcgcggtc
      361 tcgctggcgc gtgcgggcgc aagtgtcggc cttctcgatg ccgatgtcta cgggccgagc
      421 atccccctga tgatggggat ccacgagaag ccgctgatga aggacaacaa gatcgtcccg
      481 ctcgaggcgc acggcgtgaa gctgatgagc atcggcttca tcctcgattc gcagaaggcg
      541 ctgatctggc gcggacctct cgtcgcgcag ttgatcaacc agttcctcaa cgacgtgcac
      601 tggggtgatc tcgactacct cgtcgtcgat ctcccgccgg gaaccggtga cgtgcagttg
      661 acgctcgtcc agaagattcc gatcgccggg gctgtgatcg tgacgacgcc gcaggaagtc
      721 gcgctcgcgg acgcgctcaa ggggctcaag atgttccagg aggtgaagac gccgatcctg
      781 ggcatcatcg agaacatgag ctggttcatc agcccggaaa ccggcacccg cttcccgatc
      841 ttcggcgagg gcggcggtca acgcacggcc gaggaaaacg gcgtggagct tcttggccag
      901 attccgatcg agatgggaat ccgtgagggt ggcgataccg gcgccccgat ggcgtcgacg
      961 catccggaat ccgagaccgg ccaggcgttc gcgcatctcg ccgagcgcgt cgccaaccag
     1021 ctcgcggtcc aggcgatggc gaagccgcgc aagccgacga tcatgcttcg taccgtccga
     1081 taggttgccg ggcgagatcg gcgccggcgg gcccggtctg tcaaccgggg attgcccatg
     1141 ctaggatgcc accatgtctc gcgatccggc atggacaaca tgaatctatc gatcgaacac
     1201 cttcttgcag ccccgccaga tgagggaacg cttcgtcatg tgtacgaagc gttgctggat
     1261 gccgccgagg acgacatctt cctcctcgat cccgagtgcc gcgtcatcta tgtcaatgcc
     1321 ccggcgctgc gtgcgatccg gagcgcgttg ggggcggatt tcacgcggga agcgatcgtt
     1381 ggcaagactc tcgaggagtt gaactatccg gagtcgctcg ttcgtcagct ggcggagcac
     1441 gtcgggcgtg ccgtcggcgg cgaaaccgtc gatgccgaga tcccgtatcc cgccggtgtg
     1501 aatgtggaac ggctctacga gtaccggctt tccccgctgg acgagcagat acccgctccc
     1561 cttgtcatcg gaatcgctcg cgacatcgag agtcagaagg tcgatggccg cagtcgcctc
     1621 gcgctgtacg agggtgaacg gctggcgcgg gccgaggcgg aagccgcggt ccggattcgc
     1681 gacgagttcc tctccgccgc cgctcacgat ctccggacgc cactgaccgc cgcgcaggga
     1741 cgggcgcaat tgatgctccg ccaactgcag cgccaggggg gtctcgatcc cgatcggttg
     1801 cg
//
LOCUS       DASTMD010000730         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_155302, whole genome shotgun sequence.
ACCESSION   DASTMD010000730 DASTMD010000000
VERSION     DASTMD010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155302"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFQ54_10830"
     CDS             complement(<1..191)
                     /locus_tag="VFQ54_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165527.1"
                     /translation="MAGMINVGGESIDRHFLRQRIAADRLEPLALPLGLDQYEGLIDV
                     EPDQAWLPLALALIGLLRRL"
     gene            complement(1022..2203)
                     /locus_tag="VFQ54_10835"
     CDS             complement(1022..2203)
                     /locus_tag="VFQ54_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU0165528.1"
                     /translation="MSQERAAARVGIHVRTARDWDHGVRRSRRSRFYPDGRRVNYATG
                     QTTMETMTSPPPGLTRGEQTLDPRFLSLADREQIADLRLAGHGFQAIGRALGRPASTI
                     MREVDRNSVAGRYHPHAAHRLAAARRPRPKPAKLAMAGDLRTYVADRLSAHWSPEQIS
                     RTLPQQYPDDEAMRVSPETIYQAIYVQARGGLKRELTVALRSGRTRRKPHRLPEQRTS
                     PVAEPMLIISERPAAVEDRAIPGHWEGDLILGAGNRSAILTLVERSTRYVLLGHLPDG
                     HTAEEVRDILVPLMRTLPEHLRHSLTGDQGGEMAQHRTFTIATNVPVYFCDPASPWQR
                     GSNENTNGLLRQYFPKGTDLAIHSPQDLDHVADQLNTRPRKTLDWETPAQRLHHLIVP
                     A"
BASE COUNT          556 a          904 c          847 g          544 t
ORIGIN      
        1 agacgccgga ggagtccgat cagtgcgagc gctagtggca accatgcctg gtcgggctcg
       61 acatcaatca gcccctcgta ctgatctagc cccaaaggga gcgccagcgg ctcaagccga
      121 tctgccgcga tgcgctgacg caggaaatgc cgatcgattg actctccacc cacgttgatc
      181 attccggcca tgcgactccc ggcacaggac aaatcagcat agctgtgagt ctaacagaac
      241 taatgttcta tttaaagcgc ccgccagtca catcgtcgcg ggtcgtccgc ttgcgcgagg
      301 attcagcacc gcgagcgtaa tcctctccca tacatgtcgg cacgccgctt atcgcgcatg
      361 attgaatgac atgccacctg acctgctacg cgacatcggc aagtcctcca ccatctggga
      421 accctgaaag gaatcactcg gataacagca gttggttcga tgaggtcgaa gggcatgccg
      481 accttcacca atgccgactc tctcgtcgca gaacccactc actccagtgg ctgtgaatct
      541 gacagccttt catttttctt gtacaggaca gatgacgtag accgccccgg ctgatgcgtc
      601 aacctaagat cgagagacat cgccccaacg tgagccgaag ctctacctac tccgcacgat
      661 ccgagtgtga ggtcccgact gagcgtatgc cgggcatcgg tgtcaatcca gaggcaactt
      721 acggattcta ctcctcaaac tccctcgaag ggacgtcgac gcgaaccaca ccgaggagat
      781 gggccgaaag cgctgccatc ctttccaagc ccctcgttga caagtgcagc tgttataaat
      841 cccaaaaaaa actcaataag atgagacata atcgggcgat aatgtcgaaa aatatacgaa
      901 acccggctta attattgatt tcggctcttc ggacacgaag tgcattcaga caagtatttc
      961 tcgaccaagt ggtgcatccg ggaagggcag attcacgcga acatcgcaac acggagatga
     1021 gttaagccgg cacgatcaag tggtgcagac gttgggctgg ggtttcccag tccaacgttt
     1081 tgcgtggacg cgtattgagt tggtcggcga cgtggtcgag gtcttgaggg ctgtggatcg
     1141 ccaggtccgt ccccttgggg aagtattggc gcaacagccc gttggtgttt tcgttcgagc
     1201 cgcgttgcca cggactggcg ggatcgcaga aatagacggg cacgttcgtg gcgatggtga
     1261 aggtccgatg ctgggccatc tctcccccct gatccccggt gagcgagtgt cgaaggtgct
     1321 ccggcaaggt cctcatcagc gggacgagga tgtctcgtac ctcctcggcg gtgtggccgt
     1381 ccgggaggtg gccaaggagc acatagcgtg tactgcgctc cacgagcgtg aggatggccg
     1441 accgattgcc tgcgcccagg atcagatccc cttcccaatg gccggggatg gcgcggtcct
     1501 cgaccgcggc cggtcgctca ctgatgatga gcatcggttc ggcgaccggg gacgtgcgct
     1561 gttccggcag ccgatggggt ttgcgccggg ttcgtccgga tcgcagggcc acggtcagtt
     1621 cgcgcttgag tccgcctcgg gcctgtacat agatcgcctg atagatcgtc tctggactca
     1681 cccgcatcgc ctcgtcgtcc ggatactgct ggggcagcgt ccggctgatc tgttctggcg
     1741 accagtgggc gctcagtcgg tccgcgacgt aggtgcgcag atcgcccgcc atcgccaact
     1801 tggcgggttt cgggcgggga cgccgcgccg ccgcgagccg gtgtgcggca tgcgggtggt
     1861 agcggcccgc gaccgagttc cgatcgactt cccgcatgat ggtggaggcc ggtcgcccaa
     1921 gggcacgccc gatcgcctgg aacccgtgcc ccgccagccg caaatccgca atctgttccc
     1981 ggtccgccag ggacaggaat cgcggatcga gcgtctgctc cccccgggtt agcccgggtg
     2041 gcgggctcgt catggtctcc atcgtcgtct gccccgttgc gtaattcacc cggcgcccgt
     2101 cgggatagaa gcgggagcgg cgcgagcgcc gcaccccgtg atcccagtcc cgcgccgtcc
     2161 ggacatgaat cccgacccgg gccgccgccc gttcttgcga caccccctgt gcgcgcaggt
     2221 ccgcatacgc cgtccgggcc gggtgggcag accggaccga tcgcatcccg gcctgccgtg
     2281 cccatccgta ggccgtcgac tcgttcattc cgaattcccg cgcggcaaca cggacactgc
     2341 caacgagtgt caacagggcg agaaacgccg ccttctgctc cgcagcttct gaggacaaaa
     2401 ccatgatagt cgcaacctcc tgatttcagg gtgttgcgac taccactaga acctaccggg
     2461 acaaccgggc tcttcgcatt gctgtcgtgc gtagctagcg aagacgtctc cgccaggcaa
     2521 tgccaccggc accaacgaca ctcagcattg caagcaggag catgaacacg ctcgtgctgc
     2581 ttgagctgtt acctgaaccg gtgttcggga gcgccgtcac tttagtacct cctccgctgc
     2641 cggatcccgg atctcctggg gatgacgcat gtcccccatc agacggactc gttggagcga
     2701 gcgttacaat cggcgttgag gcgccgcaag ccaccacgat cgtcaccgtg cctatcacca
     2761 gctccgtatc ggccgtcttc accgtgtaca caaaactgtc ggtaccgcag aaccccggat
     2821 ctggcgtata gaggcagcga ccgtcaggat c
//
LOCUS       DASTMD010000731         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1586296, whole genome shotgun sequence.
ACCESSION   DASTMD010000731 DASTMD010000000
VERSION     DASTMD010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586296"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFQ54_10840"
     CDS             <1..334
                     /locus_tag="VFQ54_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165529.1"
                     /translation="KEIEEMRDQWNALATDVPLIIIESPYRALVGPLLTYLDELQKQA
                     PHSTLTVVLPEYVPRHWWEQILHNQTALRIKAALLFRPGTVVISVPYHLESNPPVPAR
                     INAFQRRL"
     gene            1035..2171
                     /locus_tag="VFQ54_10845"
     CDS             1035..2171
                     /locus_tag="VFQ54_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0165530.1"
                     /translation="MEHPGAFHTSDGFAARIDQHRGWLLGWFARRCGDLDTAEDLAQE
                     TTLEAWRIRTRFLTADGERAWLSAIATNVLLRWRRAMVRHRSMSLLDDSATGADDESL
                     EWWVERRELEELLDRALHALSIPSQRLLRDRYYLEQPIAEIAARNGITAQHAAVRLQR
                     ARKQLRVVVLDEFASEIGAFGCHPEARDGSQPTKFWCPLCGQHQLRMTLSPDRFSLTC
                     PSCTANPGSFLYLWDSGPDEPVLTGIRTAPQALRRTLPATSWKATPADMPHRCSKCGA
                     VSRPDFRVLPSRSADDLAALQIHFICSCDGPSFQSSTYPGMAIFHPAGTDFWRAHGKI
                     RVAGTSEITFRGMPAVGVTLAATSSSEHRFFAFSRETAHLLLVD"
     gene            2210..3262
                     /locus_tag="VFQ54_10850"
     CDS             2210..3262
                     /locus_tag="VFQ54_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0165531.1"
                     /translation="MIEVEHVSKRFTYSNPDPNSNDGDMWFDYLLGMIGRNKGVFAAR
                     RRRVTAVDDISFTVQPGEFFGLLGPNGAGKSTLVKMISTILTPTSGAIRIAGHDTRHD
                     SARARASLSVVPSNGWLSFDSHLTVSQNLEFWGRLFGMDTSIARQRTREALDAVGLGT
                     SLSSLPTALSSGMRQRLAIAKGLLVRAPVFVLDEPTANVDPISSSDIQEFIRSRLNKD
                     LGQTVLMTTHNMVEAERLCDRIGIVNRGQLVACDAPAALLRLLEGRVVELAISRDLAS
                     VAADLRTLPVIQRVQEFPTEHGRGKCRLLLRESTPSESIAKFIQGAGMSDATIRLTDP
                     DLEDLFFALTRHDVES"
     gene            3262..>3779
                     /locus_tag="VFQ54_10855"
     CDS             3262..>3779
                     /locus_tag="VFQ54_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165532.1"
                     /translation="MQTFHIAPPAGRAYLLGVLPVLLRRQAKLWLQFRISSAMDILTM
                     LAQASVFFFVGKAIGGDTWTDANAGFLAVGIVANTLLEAAVAGPYRSIANAYWSARLE
                     SMLLSPCPLILQIVADVLWILINAAISALFLAIIGASFGARLHVSIPDILLALLVVAT
                     ALVSVLGIGFVSA"
BASE COUNT          661 a         1286 c         1117 g          715 t
ORIGIN      
        1 aaaggagatc gaggagatgc gcgaccagtg gaacgcgctg gctaccgatg tgccgctcat
       61 catcatcgag tcgccgtacc gcgccctggt cggcccgctg ctgacatatc tcgacgagct
      121 gcagaagcag gcgccccaca gcacgctgac ggtggtcctg ccggagtatg tgccacgcca
      181 ctggtgggag cagatcctcc acaaccagac ggccctgcgc atcaaggcgg cgctgctctt
      241 ccgccctggc acggtggtga tcagcgttcc atatcatctg gaaagcaacc cacccgttcc
      301 ggcacggatc aacgccttcc aacgccgcct ctagcgcggt gcatcaggcc agtcacgacc
      361 agcgtggcgt ggcgcgaagg cgtttgggat aggcgtccgt tcgtagggtg ggacttttat
      421 gtcccaccct tccggatagc gcgttcgaca gatgtgtgcc ccatcccatg aacatatttc
      481 gtgcagccgg agaggccgta ccgtatggag tcggacgccc ggtacggcga cgatcccccg
      541 gcttgatcac ggcacggtaa cgtgaatcca attcggagta acgtcacccc ttagttcaat
      601 gtaattagat atatcgcgac gctatggcca aagccacatt agctggaaag gagtccttac
      661 gcgccccggc caccgggagc acgatccgtg ggcgaacgga aggcgggaca ggccgtccgg
      721 acctccggtc cggcagcggc ggtcccgccc tacgaacgac cggctgcacc gcggactacc
      781 gcatccggtc cagcagcacg ttccgcaacc gtttcggatt tcgcggtagc tgaaagggag
      841 tcctcacgcg tcccaccgaa ccggatacac ccgccgctat gatggctttc gcctatcgtg
      901 tcacgcgatg gttcaggata gctgaactta tccgtgacac gatcgccttc tcccccgaac
      961 gtgtgttatg tcccgacccc aactcgcgat aaagacgaaa gaggtgatag catcgatcgc
     1021 aacgagggac agacatggag catccgggag ccttccacac cagcgacgga ttcgccgcca
     1081 ggatcgacca gcatcgcggg tggttgctcg gctggttcgc gcgacgctgc ggcgatctcg
     1141 acaccgccga agacctcgcg caggaaacca cccttgaagc ctggcggatt cgcacgcgct
     1201 tcctgacggc ggacggcgag cgcgcgtggc tctccgcaat cgcgaccaac gtgctcctcc
     1261 gctggcgtcg agcgatggtc aggcatcgct cgatgtccct tctcgacgat tccgccaccg
     1321 gtgccgacga cgaatcactc gaatggtggg tcgaacgtcg ggaactcgaa gagcttctcg
     1381 atcgcgccct ccatgcgctg tcgatcccgt cccaacgatt gctgcgggac cggtactatc
     1441 tcgagcagcc aatcgccgag atcgcggcgc gcaacgggat caccgcgcag cacgccgcgg
     1501 ttcgcctgca gcgcgcccgc aagcaactcc gcgtcgtcgt cctcgacgag ttcgcgagtg
     1561 agatcggcgc gttcggctgc caccccgaag caagggatgg cagccaacca acgaagttct
     1621 ggtgcccgct ctgcgggcag catcagttgc ggatgacgct gtccccggac cggttctcgc
     1681 tcacctgccc cagttgcacg gcgaatccag gaagcttcct ctacctctgg gactccggcc
     1741 ctgacgagcc ggtcctcacc ggaatccgca ccgcgccgca ggcattgcga cgcacgcttc
     1801 cagcaacgag ctggaaggca acgcccgccg acatgcctca tcgctgttcg aaatgtggcg
     1861 ccgtttcacg tcccgacttc cgcgttcttc cctcacgatc cgcggacgat ctcgccgccc
     1921 tccagattca cttcatctgc agttgcgacg gaccctcatt ccagagttcg acgtacccgg
     1981 gaatggcgat cttccaccct gccggaacgg acttctggcg agcacacggc aagatccgcg
     2041 tcgcggggac atccgagatc acgttccggg gcatgcccgc ggtcggcgtc acgctcgccg
     2101 caaccagttc atccgaacat cgcttcttcg cattctcccg cgagaccgct catctcctcc
     2161 tcgtcgactg accgccgcgt tctgtcgtca tcacaagggg gagccccgca tgatcgaggt
     2221 cgagcacgtc tcgaagcgtt tcacctattc gaaccccgat cccaattcaa acgacggtga
     2281 catgtggttc gactacctgc tgggcatgat cggtcgcaac aagggcgtgt tcgccgcccg
     2341 gcgacgccgc gtgactgccg tcgacgacat ttcgtttacg gtccagccgg gggagttctt
     2401 cggattgcta ggccccaacg gcgccggcaa gtcgacgctg gtgaagatga tctcgacgat
     2461 cctcacgccc acctctggcg cgatccggat cgctggtcac gacacgcgcc acgattcggc
     2521 gcgtgcgcgg gcgtcgctct ccgtggtgcc gtcgaacgga tggttgtcgt tcgattccca
     2581 cctgaccgtc agccagaacc tcgagttctg gggacgcctc ttcggcatgg acacgtcgat
     2641 agccaggcag cgaacccgcg aggccctcga cgccgttggt ctgggcacgt cgttgtcatc
     2701 gcttccgacg gcgctctcca gcggcatgcg gcagcgcctg gcgattgcga aggggcttct
     2761 cgtccgcgcg cccgtcttcg tgctcgatga gccgacggcg aacgtcgatc cgatctcgtc
     2821 cagcgacatc caggagttca tccggagccg cctcaacaag gatctcgggc agaccgtcct
     2881 catgacgacc cacaacatgg tcgaggcgga gcgactctgc gaccggatcg ggatcgtcaa
     2941 ccgcggccaa cttgtcgcct gcgacgctcc ggccgccttg ctgcgtttgc tggaaggacg
     3001 ggtcgtggaa ctcgcgatct cccgcgatct cgcgtcagtc gccgccgacc tgcggacgct
     3061 gccggtcatc cagcgcgttc aggagttccc caccgaacat ggtcgtggaa aatgtcggct
     3121 cctgctccgg gagtcgaccc cgtccgagtc gatcgcgaag ttcattcagg gggccgggat
     3181 gagcgatgcc acgatcaggc tcaccgaccc agacctcgag gatctcttct ttgccttgac
     3241 tcgtcacgat gtggagtcct gatgcaaacc ttccacatcg cacctcccgc gggacgcgcc
     3301 tatctcctcg gtgtcctgcc tgtgctcctc cggcgacagg cgaagctctg gctccagttc
     3361 cggatcagtt cggcgatgga catcctgacg atgctcgccc aggcaagtgt tttcttcttc
     3421 gtcgggaagg ccatcggagg tgatacctgg acggatgcga atgccggctt tctcgcggtt
     3481 ggcatcgtgg cgaacacgct cctcgaggcc gcggtcgcgg gaccgtatcg ctcgatcgcc
     3541 aatgcctact ggtcggcccg tctcgaatcg atgctgctgt cgccctgtcc gctgatcctg
     3601 caaatcgtgg cagatgtcct ctggatcctg atcaatgccg cgatcagcgc gctcttcctg
     3661 gcaatcatcg gcgccagctt cggcgcacgt ctgcacgtct ccatcccgga tattctgctg
     3721 gcgcttctcg ttgtagccac ggccctcgta tccgtgctgg ggatcggctt cgtctcggc
//
LOCUS       DASTMD010000732         1952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2836327, whole genome shotgun sequence.
ACCESSION   DASTMD010000732 DASTMD010000000
VERSION     DASTMD010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2836327"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VFQ54_10860"
     CDS             <1..569
                     /locus_tag="VFQ54_10860"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0165533.1"
                     /translation="AMVFQGRSLFPWLTLRQNVAYGLKLRNVPRRERAHRADDLLAMV
                     GLAAFRRSYPHQVSEGMRQRVAIARALAVDPDILLMDEPFGALDEQTRFLLQEELLRI
                     WEQSGKTVLFITHSIDEAIVLADQIVIMSAQPGTVRATIDVPFARPRSVADIRSDPSF
                     SALFGQIWSTLRDEVTAARAAQEQALQR"
     gene            566..1336
                     /locus_tag="VFQ54_10865"
     CDS             566..1336
                     /locus_tag="VFQ54_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165534.1"
                     /translation="MRTTQRRIIEVVLTISAPVILLAIWEFLSKTKTIDPLFWPAPTT
                     LWPTATDLFEHQDLWGDIRISLIRIVVGFAVGTIPGIALGLAMGLFWPIRVFFMPLAT
                     AIYAVPKIAILPLVIIAFGIGESSKIAVVALSIFFMVVLSTMSGVLELDRTYRDVASN
                     LGASRWELFYTVALPGALPAIFTGMRLALGFSLIVIVGTEFLAADKGIGHMIWQNYQT
                     LRIKQMFVGLIITGLMGWVLTLLLDLVERFAMPWRSPE"
     gene            1339..>1952
                     /locus_tag="VFQ54_10870"
     CDS             1339..>1952
                     /locus_tag="VFQ54_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEU0165535.1"
                     /translation="MPAAAPRAHLRRNDRSFIRTWFNAVRPRSFTTSTVPIIAATALA
                     MVDGVAYPDLFIFMLLASIFTHAACNLTNDYFDDRRGVDSEMTLGQGGALQSGAITHL
                     ELRNAIIICFGLALLFAIPVIVTVGPIIILIGILSAAAAYLYTAGPFPLAYYALGEVT
                     VFFAMGIGMVCGAYYVNTGQITIPSVLLAIAVGCLAAAFLHANNV"
BASE COUNT          305 a          676 c          561 g          410 t
ORIGIN      
        1 acgcgatggt tttccaggga cggagcctct tcccctggtt gacgcttcgc cagaacgtcg
       61 cctacggact caagctccgc aatgttcccc gccgtgaacg agcgcatcgc gccgatgacc
      121 tgctcgcaat ggttggcctt gccgcgttcc gtcgttccta tcctcatcag gtgtccgaag
      181 gcatgcgaca gcgcgtcgcg atcgcccggg ctcttgcggt cgatccagac atcctgttga
      241 tggacgagcc gttcggcgct ctcgacgagc agacgcgttt cctgttgcag gaagagctgc
      301 tcaggatctg ggagcaatcc ggcaagaccg tcctcttcat cacccactcg atcgacgagg
      361 cgatcgtgct cgccgaccag atcgtgatca tgagcgcgca gcccgggacc gtccgggcga
      421 cgatcgacgt cccgttcgcc cgtccccgtt ccgttgccga tattcgctcg gatccgtcgt
      481 tctcggcgct cttcggtcag atctggtcga cgctgcgtga cgaagtgacg gctgcccgcg
      541 ccgcccagga acaggcgttg caacgatgag aacgacgcag cgccgcatca tcgaggtcgt
      601 cctgacgatc agcgcgccgg tgatcctgct cgcgatctgg gaattcctct cgaagacgaa
      661 gacgatcgac ccgctcttct ggccggcccc gacgaccctc tggccgaccg cgaccgatct
      721 cttcgagcat caggatctct ggggcgacat ccggatcagc ctgatccgca tcgtcgtcgg
      781 ctttgccgtc ggcacgatcc ccgggatcgc cctcggcctg gcgatggggc tcttctggcc
      841 gatccgcgtc ttcttcatgc cgctcgcgac cgcgatctac gccgtgccga agatcgcgat
      901 cctgccgctc gtgatcatcg ccttcgggat cggcgagtcc tcgaagattg cggtcgtcgc
      961 gctctcgatc ttcttcatgg tcgtgttgag cacgatgagc ggcgtgctcg aactggaccg
     1021 gacatatcgc gacgtcgcgt cgaatctcgg cgccagccgc tgggagctct tctacaccgt
     1081 cgcgcttccc ggggcgttgc cggcgatctt caccgggatg cgactcgcgc tcggattctc
     1141 cctgatcgtg atcgtcggta ccgagttcct cgccgcggac aagggcatcg gccacatgat
     1201 ctggcagaat taccagacac tccggatcaa gcagatgttc gtcggcttga tcatcaccgg
     1261 actgatgggc tgggtcctga ccctgcttct cgatctcgtc gaacgttttg ccatgccgtg
     1321 gcgatcaccg gaataggtat gcctgcagca gcccctcgcg cccacctccg acgtaacgac
     1381 cgctcgttca tccgaacgtg gttcaacgcc gtccgtccgc gctcgttcac gacctcgacc
     1441 gtgccgatca tcgccgctac ggcgctggcg atggtcgatg gggtcgccta ccccgacctg
     1501 ttcatcttca tgttgctggc gtcgatcttc acgcatgccg cctgtaacct gacgaacgac
     1561 tacttcgacg accggcgcgg tgtcgattcg gagatgacgc tcggccaggg cggtgcgcta
     1621 cagagcggcg cgatcaccca tctcgaattg cgcaacgcga tcatcatctg tttcggactg
     1681 gccctcctct tcgcgatccc ggtgatcgtg accgtgggac cgatcatcat cctgatcggc
     1741 atcctgagcg ccgccgccgc ctacctctat accgccggcc ccttcccgct cgcctattac
     1801 gcgctgggcg aagtcaccgt cttcttcgca atgggcatcg gaatggtctg cggggcgtat
     1861 tacgtcaaca ccggacagat cacgatcccg tccgtccttc tcgcgatcgc cgtcggctgt
     1921 ctcgccgccg ccttcctcca tgccaacaac gt
//
LOCUS       DASTMD010000733         1879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_245991, whole genome shotgun sequence.
ACCESSION   DASTMD010000733 DASTMD010000000
VERSION     DASTMD010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245991"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1002
                     /locus_tag="VFQ54_10875"
     CDS             115..1002
                     /locus_tag="VFQ54_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0165536.1"
                     /translation="MSHWSTIGQFKDWAVGTARVLSRQLRDISPIHLLHGAVVTEGVG
                     TVMVSTASTTDSHHENAHRRILLVEDEASISGFVRRGLIFEGFDVEIADDGRLALEKI
                     RDGAPDMIILDLMLPGIDGIEVAKRVRAAESMDSGPRVPILMLTARDAVPDRVAGLEA
                     GADDYLVKPFDFDELLARVRALLRRSQTQGETRKTGDVLKFEDVTLDAGSRIVTRGER
                     VIELTPREFDLLALFLHNPNQVLTRTTLMQRVWGEDFYGESNVLEVFVGNVRRALELE
                     GEPRLIQTVRGVGYVLRPR"
     gene            1002..>1879
                     /locus_tag="VFQ54_10880"
     CDS             1002..>1879
                     /locus_tag="VFQ54_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEU0165537.1"
                     /translation="MALLRGDPAKAPETTEAPAVETIEPKTHTVHPHAYSSDLPPDSS
                     FFRSIRFRLTATYAVLLIVILVALGIVLRVLLTRNLENDVNQRLLSVAQNIEANTRIE
                     LNPQPGESSIVPPSKDAYVLAGFQVGIIDTTFTSPDSATPSYSFLSDYTSFPDPTSTL
                     SQIDYDSVYTNRRAEVQQITVRNNPYRVVVYPFDPLQSGNVSGIIVVGQSSTSFAKTT
                     SLLNQVLLFVGAVGVILAVIAGWWVAGRALSPVGRITRTADEIAEDQYDVMLSKRLVV
                     SRAGDELSQLALTFNA"
BASE COUNT          353 a          621 c          543 g          362 t
ORIGIN      
        1 ggggcaattc atgccttcct cttattctca tgaaaagaag ctgggattgt gaccgtcgcc
       61 cttgtccccg atcgcacccc ctggaatccg tcgcccgcag gaggtcctcc gcggatgtcg
      121 cattggagta ccattggaca attcaaggac tgggctgtcg gcaccgcgcg ggtgctttcc
      181 cggcagctcc gcgacatctc gccgattcat cttctgcatg gggcggtggt aacagagggt
      241 gtgggaacgg tcatggtctc cacggcatcg acaaccgatt cacatcacga gaacgcacac
      301 cgccggatcc tgctcgtcga ggatgaggcg tcgatctccg ggttcgtgcg acggggcctg
      361 atcttcgagg gattcgatgt cgagatcgcc gacgacgggc ggctcgcgct cgagaagatt
      421 cgcgacggcg ccccggacat gatcatcctc gacctgatgc tgccgggaat cgacgggatc
      481 gaagtcgcca agcgagtgcg cgcggcagaa tcgatggatt cgggtccgcg cgtcccgatt
      541 ctgatgctga ccgcccgcga tgccgtgccc gatcgcgtgg ccggacttga agccggagcc
      601 gacgactatc tcgtcaagcc cttcgacttc gacgaactcc tggcgcgtgt ccgcgcgttg
      661 ctccgtcgct cgcagacaca gggcgagacg cgcaagaccg gcgacgttct caaattcgag
      721 gacgtgacac tcgacgccgg gagccgcatc gtcacccgcg gcgagcgcgt gatcgagctg
      781 accccgcgcg aattcgatct gctcgcgctc ttcctccaca acccgaatca ggtgctcacc
      841 cgcacgacat tgatgcagcg cgtctggggc gaggacttct atggcgagtc gaacgtcctc
      901 gaggtcttcg tcggcaacgt acggcgggcg ctggagctcg agggcgagcc gcgactgatc
      961 cagaccgtgc gcggagtcgg atacgttctc aggccacgat aatggccctt cttcgaggtg
     1021 acccggccaa agcgccggaa acgaccgaag cccccgccgt agagacgatc gaaccgaaga
     1081 cacatacggt tcatccccac gcctattcca gcgatctccc tcccgactcg tcgttctttc
     1141 gctcgatccg cttccgcctc acggcgacgt atgccgtcct gctgattgtg attctggtcg
     1201 cgctcggcat cgtcctgcgt gtgctgctga cgcgaaatct cgagaacgac gtcaaccaac
     1261 gactcctgtc cgtcgcccag aacatcgaag ccaacaccag gatagagctg aatccgcaac
     1321 ccggtgaatc cagcattgtt ccaccaagca aggatgcata cgtcctcgcg ggattccagg
     1381 tcggcatcat cgacacgacc ttcacgagcc cggacagtgc gacgccgagc tactccttcc
     1441 tgtccgacta cacgtccttt ccggatccga cgagcacctt gtcgcagatc gactacgact
     1501 ccgtctacac caaccgaagg gccgaagttc agcagatcac ggtcagaaac aacccttacc
     1561 gcgtcgtcgt ctatcctttc gatccccttc agtcgggaaa tgtctcgggg atcatcgtcg
     1621 tcggtcaatc gtccacgtct tttgccaaga cgacgagcct gctgaatcag gtcctcctct
     1681 tcgttggtgc ggtgggagtg attctcgccg tgatcgccgg ctggtgggtc gccggtcgcg
     1741 ccctctctcc cgtcggccgc atcacccgca ctgccgacga gatcgccgaa gaccagtacg
     1801 atgtcatgct ctcgaaacga ctggtggtat ccagggccgg cgacgagcta agccagctcg
     1861 cgctcacgtt caacgcgat
//
LOCUS       DASTMD010000734         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1140055, whole genome shotgun sequence.
ACCESSION   DASTMD010000734 DASTMD010000000
VERSION     DASTMD010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140055"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFQ54_10885"
     CDS             <1..392
                     /locus_tag="VFQ54_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165538.1"
                     /translation="EATICLLSALEMHGLTDEIPQTLDLAIPRGMRLPAGFEHVTWHH
                     FAPETFEIGREPYEVTSIGHLGVYDAPRTIVDLFRLRYREGSDLAYGALKTWLRRRES
                     QPSDLLMISRHFPQAHREIQHALEIML"
     gene            395..1306
                     /locus_tag="VFQ54_10890"
     CDS             395..1306
                     /locus_tag="VFQ54_10890"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEU0165539.1"
                     /translation="MSELPSHASASGHAYLQLRELAKRTGRQTDELLKFYALEGFLQR
                     LARSPHVEEYVLKGGLLLAAFAARRPTRDIDLQGRNTPNDVTHIVALVDEILAISIDD
                     GLAFVIGSTRGETIRDDDEYSGVRVSVDARLHNAVVDFHIDISFGDPIWPDPVPTELP
                     LLLGGNLSLQAYPLTLVLAEKTITALQRGTANTRWRDFADLTLLSAAHSIDGDEFTEA
                     LRVVATYRRVSLTLLADVLVGWAETAQRHWVIWRRRQRLELLLPENFGHILIDCTRFC
                     DPALTGQAAHLTWSPSRQRWLTDDGNG"
     gene            1620..>2122
                     /locus_tag="VFQ54_10895"
     CDS             1620..>2122
                     /locus_tag="VFQ54_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HEU0165540.1"
                     /translation="MGIFDWWKKRRQEVEDVRRLVETVAAGSGKTAPATRPPAAPARR
                     PSTAPMPRTVATGRANGRWLAQGESIEIQGYRVSGGMIYVGKSLPTQQGGPEPALIDP
                     DLRVDQHQEDTLGRGLDYWPSYAGISPKERAGYLNWLATGRRNPVVSIGFVFLYFYGL
                     ERRFLLDV"
BASE COUNT          419 a          691 c          592 g          420 t
ORIGIN      
        1 cggaggcgac gatctgtctg cttagtgcgc tcgagatgca cggcctgacc gacgagatcc
       61 cgcagacgct cgacctcgcg attcctcgcg ggatgagact ccccgcgggt ttcgagcatg
      121 tgacctggca tcacttcgca ccagagacat tcgagatcgg tcgcgaaccc tatgaagtga
      181 catcgattgg acatctcggt gtctatgatg cccctcgtac gatcgtcgat ctgtttcggc
      241 tgcgataccg cgaaggatca gatctcgcat acggcgcact gaagacatgg ttacgtcgtc
      301 gagagtctca accatccgat ctcctgatga tctctcgcca ctttccccag gcacatcgcg
      361 agattcagca cgccctcgaa atcatgctgt gacgatgagc gaacttccat cgcatgcttc
      421 agcgtcaggc catgcctatc tccaactccg agagttggca aaaaggaccg gacgccaaac
      481 cgatgaactc ctcaagttct acgccctgga ggggtttctg caacgactcg cgcggtctcc
      541 ccatgtcgaa gaatatgtcc tcaaaggtgg gttgttgctc gccgcctttg ccgcccgtcg
      601 accgacacgg gatatcgacc tccagggaag aaatacgccc aatgatgtta cccatattgt
      661 cgcgctagtc gacgaaatcc tcgccatctc catcgatgat gggctggctt tcgtcatcgg
      721 ctcgacccgt ggcgagacaa tccgtgacga cgacgagtac tcaggtgtca gagtctcggt
      781 cgacgctcga ctccacaacg ccgtggtgga tttccacatc gacatcagct tcggcgaccc
      841 gatctggccg gatccagtcc ctacggagtt gcctctcctc ctcggtggca atctatcgct
      901 ccaagcctat ccgctcactc tggtactggc agagaagacg atcacggcac tgcaacgggg
      961 aaccgccaac acacgatggc gtgacttcgc cgatctcacg ctcctgagcg ctgcgcattc
     1021 cattgatgga gacgagttca ccgaagcact acgagtcgtc gcgacctacc ggagggtttc
     1081 gctaactctg ctcgccgacg tgctggtcgg gtgggctgaa acggctcagc gtcattgggt
     1141 gatatggcgt cgacgtcaac gtcttgaatt actcttgcca gagaatttcg gacacattct
     1201 gatcgattgc acgcgattct gcgatcccgc actcaccggg caggcagctc atctcacgtg
     1261 gtctccgtca cggcaacggt ggctgaccga tgacgggaac gggtaataca ctgccttcac
     1321 cctcgcgtcg ccaatgtccc ccaacatata caaatctgtg atacctgcat acgttggaga
     1381 acggaccatt ccgcctggtc agacggatct gaaaggcggc cgcaacgaga gcgagcacga
     1441 ggctcgcccc cctatggaga gaccgactcc cgttgaggtg gcctcatgct cgcccggcgc
     1501 caccagcagc ggcgccaggt catccatcca cccgacagcc ggcgcgcgcg atcccggcat
     1561 cgacggtagt attacgtcgt gttgataccg gctgcgtgac tgatcggcgg agggttatcg
     1621 tgggcatctt cgattggtgg aagaaacgac gccaggaagt cgaagatgtc cgacgccttg
     1681 tcgagacggt tgccgccggt tcgggcaaga ccgccccagc gacccgcccg cctgccgccc
     1741 cggcgcgacg tccctcgacc gcgccaatgc cgcgaaccgt tgccactggc cgcgccaacg
     1801 gacgctggct cgcacagggt gaatcgatcg agatccaggg ctatcgcgtt tcgggcggca
     1861 tgatctacgt cggaaaatcg ctgccaaccc agcagggcgg tcccgagccc gcgctcatcg
     1921 atcccgacct ccgcgtcgat caacaccagg aggatacgct cggccgcggt ctcgactact
     1981 ggccctccta cgccgggatc tcgccgaagg agcgcgccgg ctatctcaac tggctcgcga
     2041 ccggtcgccg caacccggtg gtatcgatcg gcttcgtctt cctctacttc tatggactcg
     2101 aacgccgctt cctgctcgat gt
//
LOCUS       DASTMD010000735         1922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2659162, whole genome shotgun sequence.
ACCESSION   DASTMD010000735 DASTMD010000000
VERSION     DASTMD010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2659162"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFQ54_10900"
     CDS             <1..614
                     /locus_tag="VFQ54_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165541.1"
                     /translation="PFWGNTLRWSLPDPENRAFTVSASRDGDDALIAIESSSATGPSI
                     NLDGQQVEVTGPDGGSSSKVTLVASGPGQYTARLAGAQPGAYSVTLPSGTAGQTAAYG
                     MAVAPSAEWQPSTDGTVLLQAIALRTGGQVLSLDSSATSDLFAAPSSAGNQALGSVHP
                     IWQYPLVAALVIYLIDIAGRLGFNWRVGMDAIQHLAWWKRASS"
     gene            644..1609
                     /gene="corA"
                     /locus_tag="VFQ54_10905"
     CDS             644..1609
                     /gene="corA"
                     /locus_tag="VFQ54_10905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEU0165542.1"
                     /translation="MVSVQLCVDGEGKPRDITAPHEVRTILEDDRAVVWLNVARSGAQ
                     LGELGERFGFHGLAIEDATDENERPKATIYRDHVFVLLYALAYKTGNVITTPIGIFVG
                     KNYLVTVTDQPTAEFDEVSHRWHEFSDVIADRTAGSLAYALIDAIVDGYFPVIDQISE
                     RLERLETGLIERTLHNPQQIIHDTRTQLIQLRRVVSPEREAINTLLRRDVPVFDDHVS
                     EYMDDVYDHILRAIDWIDTYRDLLSNLSDLQFSVNSHKLNQTMRTMTAWSIIFMAVTL
                     IASIYGMNFDHMPELHLLLGYPAALLGMVIIGGGIFVFFHRREWL"
     gene            1664..>1922
                     /locus_tag="VFQ54_10910"
     CDS             1664..>1922
                     /locus_tag="VFQ54_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0165543.1"
                     /translation="MISSVAILSDIHGILPALDAVLAEPDVIAAERMVLPGDVAAGPQ
                     PIAVLDRLVELGDRAIWVRGNADTEMANVARGRIASSGIPIS"
BASE COUNT          332 a          675 c          555 g          360 t
ORIGIN      
        1 cgcccttctg ggggaacaca ctgcgctggt cgctgcccga cccggaaaac cgcgccttta
       61 cggtttccgc gtcacgggac ggcgacgacg cgttgatcgc catcgagtcg tcatcggcga
      121 caggaccttc gatcaacctc gatggccagc aggtcgaggt taccgggccc gacggcggat
      181 cctcgtcaaa ggtgaccctg gtcgcatcgg ggcctggcca gtacaccgcc cgccttgccg
      241 gcgcacagcc cggcgcctat tcggtgacgc tcccctcggg aacggccggc cagaccgctg
      301 cttacggcat ggccgtcgcg ccttcggcgg aatggcaacc ctcgacggat ggaaccgtgc
      361 ttctgcaggc catcgccctc cgtaccggcg gtcaggtcct ctccctcgac agcagcgcca
      421 cgagcgatct cttcgccgcg ccgtcgtcgg ccggcaatca ggcgctggga tcggtccatc
      481 cgatctggca gtatccgctt gttgccgcgt tggtcatcta tctcatcgac attgcgggtc
      541 gactcggctt caattggcga gtcgggatgg acgccattca acacctcgcg tggtggaagc
      601 gtgcatccag ctagtcccct gacctcaacg atccggagcg gccgtggttt ccgtacaact
      661 ctgcgtggat ggcgagggaa agcctcgcga tatcaccgcg ccgcacgaag tccgcacgat
      721 actcgaggat gaccgcgcgg tcgtctggct caatgtcgcc cgctccggcg cgcaactcgg
      781 cgaactcggc gagcgattcg ggttccacgg tctcgcgatc gaggatgcaa ccgacgagaa
      841 cgagcggccg aaggcgacga tctatcgcga tcacgtcttc gtgctcctct acgccctcgc
      901 ctacaagacc gggaatgtca tcacgacgcc gatcggtatc ttcgtcggca agaactacct
      961 agtcaccgtg accgaccagc cgaccgccga gttcgatgag gtctcgcatc gctggcatga
     1021 gttcagcgac gtcatcgccg accggaccgc gggctccctc gcctacgccc tgatcgatgc
     1081 catcgtcgac gggtacttcc cggtgatcga ccagatcagc gagcgtctcg aacggctcga
     1141 gacggggctc atcgagcgca cgctccacaa cccgcagcag atcatccacg atacgcggac
     1201 ccagctgatc cagcttcgcc gggtcgtctc gccagaacgc gaggcgatca acaccctcct
     1261 ccggcgcgat gtccccgtct tcgacgacca cgtctcggaa tacatggatg atgtctacga
     1321 tcacatcctg cgcgcgatcg actggatcga tacctaccgc gatctgctct cgaacctttc
     1381 cgacctgcag ttctcggtga actcccacaa gctgaaccag acgatgcgga cgatgaccgc
     1441 gtggtcgatc atcttcatgg ccgtcacgct catcgcgagc atctacggca tgaacttcga
     1501 tcacatgccc gaactgcatc tgctgctcgg atatcccgcc gcattgctcg gcatggtcat
     1561 cattggtggc ggcatcttcg tcttcttcca ccgtcgtgag tggctctgat cctgcggctt
     1621 cggcaacacg acgccatccg cggcgtctga aaggaccgtc ccgatgatct catccgtcgc
     1681 catcctctcc gacatccatg gcatcctccc cgccctcgat gccgttcttg ccgagccgga
     1741 cgtgatcgcg gcagaacgca tggttcttcc cggcgacgtc gccgccggcc cccagcccat
     1801 cgccgtcctc gatcgtcttg tcgagctcgg ggatcgcgcg atctgggtac gcggcaacgc
     1861 ggataccgag atggcgaacg tcgcccgggg gcgcatcgca tcctcgggaa tcccgatctc
     1921 gg
//
LOCUS       DASTMD010000736         3027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_692347, whole genome shotgun sequence.
ACCESSION   DASTMD010000736 DASTMD010000000
VERSION     DASTMD010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692347"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1621)
                     /locus_tag="VFQ54_10915"
     CDS             complement(<1..1621)
                     /locus_tag="VFQ54_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0165544.1"
                     /translation="MTMQTGDEKFHVLSQRTLRLASTRRRLLGAAGGLGGALLLGGAG
                     AGASGTRSARYAQEQAQDALEIIIGTLGEANTINPFKTSDSEGDWRAIQLFDELIRID
                     PATYAPKPGLASEFTVDNLTINLKLQPNATFSDGTDLTADDVAFTIAGHLNPKTASVR
                     AAKFLSIAGAQEYVDGKATTVSGIKVVDPKTLTITLAKPEAAILYNLRFIRVVPKAQL
                     DGKDLANDPWFQKPVGAGPFAFDSWTTDADFVMTRNEHFWEADKPTIAKVTHRVIADA
                     QSLVLALLNNEIDGSNYPSPSGKAELEKNPDLAIIIPPFSSPNGWSFNCRTGPLAKKE
                     VRKAIAMALDNEQFAADSLLGIGKAGIGPIAPDSWAFDKTLKPIPFDVDGAKKLVQDA
                     GVEGSEITFTVNQGNIQREDWLTYTQQALEAIGLKVKAEAIEYATLVDRVTGSSHDFG
                     ASNGDFAGVTAEPSELFDQFHSTGASNYTGFSTPELDGLLEKARTTLDLEAAKPIYAQ
                     IQAILMDEVPVDWAWYRPFLHTVNKKWTGYVD"
     gene            1974..2519
                     /locus_tag="VFQ54_10920"
     CDS             1974..2519
                     /locus_tag="VFQ54_10920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0165545.1"
                     /translation="MAAMAHANIIIRNAREADMSAVREIYAEHVLHGLATFEEVAPTV
                     EEMISRRAVAATLGLPYLVAERDGEVVGYSYATIYRPRPAYRFTIEDSVYVRDGLGGR
                     GIGSLLLGELIARCEQGPWRQMIAVIGNSENIGSIALHARFGFEHVGILRSVGFKLGR
                     WVDTVLMQRALGSGDAVTPVP"
     gene            complement(2525..2869)
                     /locus_tag="VFQ54_10925"
     CDS             complement(2525..2869)
                     /locus_tag="VFQ54_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEU0165546.1"
                     /translation="MTGQAFEGQAVRIRIYFGERDHQGMRSLWSAMLEFLRKEGAPGA
                     TVIRGIAGFGAHSKVHTASIVDLSDDLPLVLEWIDTEEQVQRLLPHIEAMLDGGLITS
                     DPVTIIRYQPGD"
BASE COUNT          532 a          922 c          980 g          593 t
ORIGIN      
        1 tgtcgacgta gccggtccac ttcttgttca cggtgtgcag gaacggccgg taccacgccc
       61 agtcgaccgg gacctcatcc atcaggatcg cctggatctg tgcgtagatc ggtttcgccg
      121 cctcgagatc gagcgtggtg cgcgccttct ccagcaagcc gtcgagttct ggtgtcgaga
      181 agccggtgta gttcgaggca ccagtggagt ggaactgatc gaagagttcg ctcggttccg
      241 ccgtgacacc ggcaaagtcg ccgttcgagg ccccgaagtc atgacttgag cccgtgaccc
      301 ggtcgaccag cgtcgcgtat tcgatcgcct cggccttcac cttgagcccg atcgcttcca
      361 gcgcctgctg ggtataggtc agccagtcct cgcgctggat attcccctgg ttgacggtga
      421 aggtgatctc ggatccctcg acgccggcat cctggaccag cttcttcgcg ccatcgacat
      481 cgaaggggat cggcttcagg gtcttgtcga aggcccagct gtccggcgcg atcggaccga
      541 tgcccgcctt gccgataccg aggagcgaat ccgcggcaaa ctgctcgttg tcgagcgcca
      601 tcgcgatcgc cttgcggact tccttctttg ccagcggacc cgtgcggcag ttgaaggacc
      661 agccgttcgg cgacgagaag ggcgggatga tgatcgcgag atccgggttt ttctcgagct
      721 cggccttgcc actcggcgac ggatagttcg agccgtcgat ctcgttgttg agcagggcga
      781 ggacgaggct ctgggcatcg gcgatcacgc ggtgggtcac cttcgcgatc gtcggcttgt
      841 ctgcctccca gaaatgctcg ttcctcgtca tcacgaagtc ggcatcggtc gtccaggagt
      901 cgaacgcgaa ggggccggcg ccgaccggtt tctggaacca gggatcgttg gcgagatcct
      961 tgccgtccag ttgcgccttg ggaacgaccc ggatgaagcg gaggttgtag aggatcgccg
     1021 cttccggctt ggcaagcgtg atcgtgagcg tcttcggatc gacgaccttg atgccgctga
     1081 ccgttgtcgc cttgccatcg acatactcct gggcgccggc gatcgagagg aatttcgcgg
     1141 cgcgaaccga ggcggtcttc gggttgaggt ggcccgcgat cgtgaaggcg acatcatcgg
     1201 cggtcagatc cgtgccgtcc gagaacgtcg cgttcggctg cagcttgaga ttgatcgtca
     1261 ggttgtcgac ggtgaactcg ctggcgagac ccggcttcgg ggcatacgtc gcgggatcga
     1321 tccggatcag ttcgtcgaag agctggatcg cgcgccagtc accttcggaa tcggaggtct
     1381 tgaacgggtt gatcgtgttc gcttcgccga gcgtcccgat gatgatctcg agcgcgtcct
     1441 gcgcctgttc ctgcgcgtag cgagcggagc gggtaccgct cgcacccgct ccggcgccac
     1501 cgaggagcag ggccccaccg agaccgccgg ccgcgccgag cagacggcga cgcgtgctcg
     1561 cgaggcgtaa cgtacgttga ctgaggacgt ggaacttctc gtctccggtt tgcatcgtca
     1621 tggttcctcc tgcgagtgat ggcgacaaac gcgtgggcat caccgtcagg cggcgacgca
     1681 ttcactggga cccgacagat tttcgatacg tccatttcca tatcgaggct aacagagcac
     1741 aaagaccgat gtcaatttgg attgacgcga acgatatgtc ggtgatatcc cgaaaaacgg
     1801 ccagcatcca cgaacattgt gacaatcatc acgtatcgtc acggtcattc tgtcgttacg
     1861 cacagtttcg agggagtgaa ggcgccccgc cccgagaccg tgtggcccag gtcgcgtact
     1921 acgatggcgg gaacatgtcg tagcggaggg ctggaacgat cggaggtaac cacatggcgg
     1981 cgatggcgca cgcgaacatc atcatccgga acgcgaggga ggcggacatg tccgccgtgc
     2041 gggagatcta cgcggaacac gttctccacg ggctcgcgac gttcgaggaa gtggcgccga
     2101 cggtcgagga gatgatctcc cgccgcgcgg tggcggcgac cctcgggctt ccctatctcg
     2161 tcgccgaacg cgacggcgag gtcgttggct acagctatgc gacgatctat cgtccgcgtc
     2221 ccgcctatcg cttcacgatc gaggattctg tctacgtccg ggatggtctc ggagggagag
     2281 ggatcggcag cctgctgctc ggggaactca tcgcccgctg cgagcaagga ccgtggcgcc
     2341 agatgatcgc cgtgatcggc aacagcgaga acatcggctc gatcgcgctg cacgcgcgtt
     2401 tcggcttcga gcacgttggc atcctgcgtt cggtcggctt caagctcggt cgctgggtcg
     2461 ataccgtgct catgcaacgc gcgctcggca gcggcgatgc ggtcacgccg gtgccctgat
     2521 cggctcaatc gcccggctgg taacggatga tcgtgaccgg atcggacgtg atcaggccac
     2581 cgtcgagcat cgcctcgatg tgcgggagca gtcgctgcac ctgctcctcg gtatcgatcc
     2641 attccagtac gagtggcaga tcgtcggaaa gatccacgat cgatgccgta tggaccttgc
     2701 tgtgcgcccc gaacccggcg atgccgcgaa tcaccgtcgc gccgggtgcg ccctccttgc
     2761 ggaggaattc gagcatcgcg ctccagagcg aacgcatccc ctgatggtcc cgctcgccaa
     2821 aatagatgcg aatgcgaacc gcttgccctt cgaatgcctg accggtcatc gccgtgcctt
     2881 tccacgaatc gttcggattc cgtggagatc gcatatcgcg cgccagtctc cgggcgcttt
     2941 cggaattcac aggtatcccg tcctcgggta tcgtcagacg agactgagac agaccggacc
     3001 aacggaaagg ggcgtgcaat ggcgatc
//
LOCUS       DASTMD010000737         6558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2210896, whole genome shotgun sequence.
ACCESSION   DASTMD010000737 DASTMD010000000
VERSION     DASTMD010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210896"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFQ54_10930"
     CDS             <1..195
                     /locus_tag="VFQ54_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165547.1"
                     /translation="SSVPITGTPESIARAYPVSRMEESVAPLSQQPEELAVWQQWPVV
                     ALLAVALAIAVVIAVLAVAL"
     gene            complement(208..2268)
                     /locus_tag="VFQ54_10935"
     CDS             complement(208..2268)
                     /locus_tag="VFQ54_10935"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU0165548.1"
                     /translation="MPNRLALETSPYLLQHKDNPVDWYPWGPEALALARDRDIPILVS
                     IGYSACHWCHVMAHESFENAEIAAYMNANFVNIKVDREERPEIDTIMMTAVQAMGGRG
                     GWPLNAFLTPEGVPFYGGTYWPQSDRMGMPGFPRVLASVIDAWTTNRDGILDSADRVR
                     SFLDDSNEAAPEPGVLSPELADVALANAERQFDREYGGFGGAPKFPQVSILEFLLRHH
                     RRTGSGSALEMVLTTLDRMADGGINDQIGGGFARYSVDRIWLVPHFEKMLYDNAQLLS
                     IYIDAFRITGNVRYRKVAEEIIGWLRREMMELPTSLFAASLDADSEGVEGKYYVWSEE
                     AFERALAPSLDAEEIDLVKLHFGVTADGNFEGKNILAIVRPVATLAGQLGRETSAVEA
                     VVERARNALLTSREERVAPGKDQKVVTAWNGLTLKALAAAGSVFQDAELLAVAGRCAG
                     DILNHLRTDDRRLARSWRDGTLRGQGTLEDYAFLAEGLLELYAASGSVQWLDAAGELV
                     DRIVASFVHPTGAGFYDTADDHEDLITRPRTLQDGATPSGNAVACELLLTFGMLRNDD
                     RLLDLAERTLASLARPMREQPVFMGRHLAVLERWLSPRRDLVLAGEPTSAEFIALRAE
                     FRQRYEPHVVLGYGNPEAEQRYPLLADRPAKGNAAAYLCEHFTCLPPVTSPEALRDLL
                     DAPT"
     gene            2369..3247
                     /gene="proC"
                     /locus_tag="VFQ54_10940"
     CDS             2369..3247
                     /gene="proC"
                     /locus_tag="VFQ54_10940"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU0165549.1"
                     /translation="MSAIGVETGKQTSVLASSRLAIVGAGVMAESIVAGLLARRMVEP
                     AQIVASHPRADRRARLETQFGISTVAENADAIVDADLILLTIKPQVLAAVMAELKERL
                     SPGQVVISILAGANVETLQRGLDHDAIVRVMPNTPAQIGEGMSVWCCTESVDEVQQAK
                     VRAALGALGEELQVETEKYVDMATALSGTGPTYVFLMMEALVDAGVHMGFPRRIAEQI
                     VLQTVSGSVAFARSSGKHLAELRNMVTSPGGTSAEAIYQMEKGGLRTVLSKSVYAAYQ
                     RTQTLAAEQEEKIGGH"
     gene            3578..3973
                     /locus_tag="VFQ54_10945"
     CDS             3578..3973
                     /locus_tag="VFQ54_10945"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEU0165550.1"
                     /translation="MREQDLRIIAQFRAEGGLTMGQMDVLLLHHTGAKSELDYVTPLS
                     YVMQAGDWIIIAGNSGLPTNPGWYFNLKAFPLTEIEVGAATIPVTATEITGDERDAVW
                     AELCASRPDLAGFQNGLDRVIPLFRLTPR"
     gene            complement(4185..4928)
                     /locus_tag="VFQ54_10950"
     CDS             complement(4185..4928)
                     /locus_tag="VFQ54_10950"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU0165551.1"
                     /translation="MPVYRDYFRRLYDQIEHVVSSEGAHIETAASWMAASIASDGIVH
                     LFGSGHSHMIAEEVFHRAGSLMPLNPMLDVNLTFLGSVNATLLERTPGYGRVVVGSHD
                     IRPGEVVIVISNSGVNPVPIEVALEAKALGAQTIAITSDENYRQAPSRHASGKRLAQV
                     VDLTIDTLVPAGDALIEIPGNFARTGGVSSVIGISLINAIVAETADRLQKMGITPPII
                     PTMNLPGGDEAMEALLERYAARLPLLKRA"
     gene            complement(5193..>6558)
                     /locus_tag="VFQ54_10955"
     CDS             complement(5193..>6558)
                     /locus_tag="VFQ54_10955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165552.1"
                     /translation="TELILFRGLQGIAGGILMTNAFAIIGSIFPPAERGKWQGVTGSV
                     FGFSSVAGPLLGGFFTDGPGWRWVFYINIPVGILAAIVLMSALPKIQAKNHLPIDWFG
                     ALGIIGSAVPLLLAFSWAGTEYPWGSVQVVGMLVLAAVFAIFFLWNESRAVDPILPLE
                     MFRNRTFAIAVPTVFFIGAGMFGSISYIPLFMQTVVGVSATNSGIVLAPLMLSLVVAS
                     TTGGQIISRTGRYKWAVILGLSTMTIGLFLQSRMNINTTQGQAIVNMIFIGLGVGFVM
                     PTMNLAVQNAFPVKRIGTVTASVQFFRSIGATIGIAVMGTVMSSGLKNGLQSDLPPDV
                     SAKLPSGLVGSLNPQTLGSPESKDALRTQLSSLPDSAQLYDQLTHAMRLSIADAMHSM
                     FLLAAVGAVFALLIGVFVPENPLRKKNTPDAGAGADQTSTAAIESPALAGATASSSSG
                     GGND"
BASE COUNT         1173 a         1985 c         2226 g         1174 t
ORIGIN      
        1 tcctccgttc ccatcacggg cacgcccgag tcgatcgcgc gcgcgtatcc ggtatcacgg
       61 atggaggagt ccgtcgctcc gttgtcccag cagccggagg aactcgccgt ctggcagcaa
      121 tggccggtcg ttgccctgct tgcggtggcg ctggccatcg ccgtcgtcat cgcggtcctt
      181 gcggtggcgt tgtagtcatc tctgcggcta cgtgggagcg tcgaggaggt cgcgaagtgc
      241 ctccggtgac gtcacaggtg gcaaacaggt gaagtgctcg cagaggtagg ctgctgcgtt
      301 tcctttggcg ggccgatccg cgagcagggg ataccgttgc tccgcttccg gatttccgta
      361 gccaaggacg acgtgcggct cgtaccgctg ccggaactcc gctcgcagcg cgatgaattc
      421 cgcgctcgtc ggttctccgg cgagcaccag gtcgcgccgc ggactcagcc agcgctcgag
      481 cacggcgaga tggcggccca tgaagacggg ttgttcccgc atcggtctcg ccagtgaggc
      541 gagtgtccgc tccgccaggt ccagcaggcg atcgtcattg cggagcatgc cgaatgtcag
      601 gaggagctcg caggcaaccg cgttgccgga aggcgtcgcg ccgtcctgaa gggttcgtgg
      661 tcgcgtgatc agatcttcat gatcgtcggc agtgtcgtag aagccggcgc ccgtcggatg
      721 gacgaaggac gctacgatcc gatcgaccag ttcgcctgcc gcgtcgagcc attgcactga
      781 acctgatgcg gcatagagtt cgaggagtcc ttccgccagg aaggcgtaat cctcgagcgt
      841 gccctgcccg cgcagcgtgc cgtcacgcca cgatcgcgca agccgccggt cgtcggtgcg
      901 aagatggttg aggatgtcgc cggcacagcg cccggcgacg gcgaggagtt ccgcgtcctg
      961 gaagaccgat ccggcggcgg ccagcgcctt gagcgtgagg ccgttccacg ccgtcacgac
     1021 tttctggtcc ttgccgggcg ccacgcgctc ctcccgtgac gtgaggagtg cattccgcgc
     1081 gcgttccacg actgcttcaa ccgcggacgt ctctcgtccc aattggccgg cgagcgtcgc
     1141 gacaggacgg acgatggcga gaatgttctt gccctcgaag ttcccatccg ccgtgacgcc
     1201 gaagtgcagc ttgacgagat cgatctcctc ggcgtcgaga gacggcgcga gcgcccgttc
     1261 gaacgcttcc tccgaccaga cgtagtattt cccctcgacg ccttcgctgt cggcgtccaa
     1321 cgacgccgcg aagaggctgg tcggaagctc catcatctcc cggcgaagcc agccgatgat
     1381 ctcctccgcg acctttcgat atcggacgtt gccggtgatg cggaaggcgt cgatgtagat
     1441 gctgaggagc tgggcgttgt cgtagagcat cttctcgaaa tgcggaacga gccagatgcg
     1501 gtcgaccgaa tatcgtgcga agccgccgcc gatctggtcg ttgattccgc cgtcggccat
     1561 gcgatcgagc gtcgtgagca ccatctcgag tgcggagccg gagccggtgc gtctgtgatg
     1621 gcgcagcaga aactcgagga tcgagacttg agggaacttc ggggcgccgc cgaatccgcc
     1681 gtattcccga tcgaactggc gctcggcgtt ggcgagcgcc acgtcggcga gttccggcga
     1741 aaggacgccg ggctctggcg ccgcttcgtt cgagtcatcg agaaagctgc gaacgcgatc
     1801 ggcgctatcg aggatgccgt cgcggttcgt cgtccatgcg tcgataacgg aagcgagaac
     1861 ccggggaaaa cccggcatcc ccatgcggtc gctctggggc cagtacgtac ctccatagaa
     1921 cggcacgcct tccggcgtca ggaaggcatt gaggggccag ccgccgcgac cgcccatcgc
     1981 ctggacggcg gtcatcatga tggtatcgat ctcgggccgt tcctcgcgat cgaccttgat
     2041 gttcacgaag ttggcgttca tgtaggccgc gatttcggca ttctcgaacg actcgtgcgc
     2101 catcacgtga caccagtggc aggccgagta gccgatactg accaggatgg ggatgtctcg
     2161 atcgcgcgcc agcgccaacg cttcgggacc ccagggatac caatccacgg gattgtcctt
     2221 gtgttgaagc agataggggc tggtttcgag ggcgagacgg ttgggcacgg cgtattctcg
     2281 cttccatgag ggatatatcg gtcgttgcga tgtcatcgca atcatcctac cgtcatcgta
     2341 cggtcggtgc atgaatcagg agttgcagtt gtcagcgata ggcgtggaaa cagggaagca
     2401 gacgagcgtg ctggcatctt cccggctcgc gatcgtgggc gcgggggtga tggcggaatc
     2461 gatcgttgcc gggttgctgg cgcgcaggat ggtcgagccc gcccagatcg tcgcaagtca
     2521 tccgcgagcg gaccgacggg ccaggctgga gacgcagttc gggatctcga ccgtcgccga
     2581 gaatgcggac gcgatcgtcg acgcggatct gatcctgttg acgatcaagc cgcaggtgct
     2641 tgccgcggtg atggcggaac tgaaggaacg gctgtcgccc ggtcaggtcg tgatttcgat
     2701 tcttgccggc gcgaatgtcg agaccctgca acgcggactc gatcacgacg cgatcgtccg
     2761 cgtgatgccg aacacgccgg cgcagatcgg cgaggggatg tcggtctggt gctgcaccga
     2821 atcggtcgac gaggtgcagc aggcgaaggt gcgggcggcg cttggcgcac tcggcgagga
     2881 actgcaggtc gagaccgaga agtacgtcga catggcgacg gcgctttccg gaactggccc
     2941 gacctacgtc ttcctgatga tggaggcgct cgtcgacgcc ggtgttcaca tggggtttcc
     3001 gcgccggatc gcggagcaga tcgtcctgca gacggtctcg ggatcggtcg ccttcgcccg
     3061 gagttccggc aagcatctcg ccgaactgcg caacatggtg acatcgcctg gcgggacgag
     3121 tgccgaggcg atctaccaga tggagaaggg tggcctgcgt accgtgctca gcaagtccgt
     3181 ctatgccgcc tatcagcgga cgcagacgct ggcagccgag caggaagaga agattggtgg
     3241 ccactaggga cgagttcgct ttctcactgg gggcatgcct gcgtcgtagt gggccacccc
     3301 gtgtggccca tcgcgcgcag cgcgatatga tgcgttcatt ggaaactcct cggccaccac
     3361 ggacctccga agaaccgtag acgcggccct tgtggccgct gcgcgtcggt gaagccgctc
     3421 gcgttcgcgg cgggatctga atccccgctc tacaaacccg gtcctgtgag aaccgggtga
     3481 tgggaacgat ggtgttggag gccgcctgcg gcgacacgcg cttcgcgcgg ggatttctcg
     3541 gcaggctcga aatgacagtt gtcaggagat agacaggatg cgagagcagg atctcaggat
     3601 catcgcgcag tttcgagcgg agggcgggct gacgatgggt cagatggacg tccttctgct
     3661 ccatcacacc ggtgcgaaat cggagctcga ctatgtgacg ccgctctcct acgtcatgca
     3721 ggctggcgac tggatcatca tcgccggtaa cagcggcctg ccgacgaatc ccggctggta
     3781 cttcaatctg aaggcatttc cgctgacgga gatcgaagtc ggtgcggcga cgatcccggt
     3841 cacggcaacc gagatcaccg gcgatgagcg cgatgctgtc tgggccgaac tgtgcgcatc
     3901 acgcccggac ctcgcaggat tccagaacgg tctcgaccgg gtgatcccgc tcttccggct
     3961 taccccgaga tagacgcgac cggaacgtcg gcttcgactt cggcaagcgg ggacaagccc
     4021 cgcggggcga cgatcggcga catccgtccc cacggccctt gtggccgtaa aaccccaacg
     4081 cctgtgtcga gcgccgtgac cacaagactg tgatcgaggc cggttggctt cgacactggc
     4141 atgcggagac gagatcgcct ccctatcgcc cgaaggggga tcggtcaggc gcgcttgaga
     4201 aggggaaggc gtgccgcgta gcgttcgagg agcgcctcca tcgcctcgtc accgccaggc
     4261 aggttcatcg tcgggatgat cggcggcgtg attcccatct tctgcaagcg atctgctgtc
     4321 tcggcgacga tcgcattgat caggctgatg ccgatcacag aggagacacc gcctgtccgc
     4381 gcgaagttgc cgggaatctc tatcagcgcg tctccggcgg gaacgagggt gtcgatcgtc
     4441 aggtcgacga cctgggccag tcgcttgccc gaggcgtggc gggagggagc ctgccggtag
     4501 ttctcgtccg acgtgatcgc gattgtctgg gcgccgagag ccttcgcttc gagggcgacc
     4561 tcgatcggca ccggattgac gccggaattg ctgatcacga tgacgacctc gcccggccgg
     4621 atgtcgtgcg agccgacgac cacgcgtcca tagccagggg ttcgttcgag cagcgtggcg
     4681 ttgaccgaac cgaggaaggt gaggttgacg tcgagcatcg gattcagggg catcaggctg
     4741 ccggcccggt ggaagacttc ttccgcgatc atgtgcgagt ggccgctgcc gaagagatgg
     4801 acgattccgt cgctcgcgat gcttgccgcc atccagctgg ccgcggtttc gatgtgcgcg
     4861 ccttccgagg agacgacgtg ctcgatctga tcgtagagac ggcggaaata gtcgcggtag
     4921 acgggcatag gcaggcaatc atcctcgatc gtgcgcttag cgataccact ccgcctgggc
     4981 cgagtacatg cgggcgtagg cgccattgta cgcaatcagg tcatcatggc aaccgtcctc
     5041 gatgacgcgc ccgtccccca cgacgacgat tcgatcggca tgcctcgccg gcgatcccgt
     5101 gcgtggcggg atgattcccg gatcgttccg gacatgcaga tgcccgcacc tttcgatgcg
     5161 ggcatctgcg agtgtccgat gtgggtggag agctagtcgt tgccgccccc cgacgaggag
     5221 ctggcggtgg cgccggcaag tgcgggggat tcgatcgcgg cggtacttgt ctgatccgca
     5281 ccggcgccgg catccggcgt gttcttcttg cggagtggat tctccggcac gaagacgccg
     5341 atcagcagcg cgaatacggc tccgaccgcc gcgaggagga acatcgagtg cattgcgtcg
     5401 gcgatgctca ggcgcatcgc gtgcgtcagc tggtcgtaga gctgggcgga gtcggggagc
     5461 gacgagagtt gcgtcctcag cgcgtccttg ctctcggggc tgccgagtgt ctgcggattc
     5521 agcgaaccga ccaatccgga tggaagcttc gccgagacgt ctggcggaag gtcgctctga
     5581 agcccgttct tgagcccgct gctcatcacc gttcccatga cggcgatgcc gatcgtcgcg
     5641 ccgatcgagc ggaagaactg gaccgaggcg gtgacggtgc cgatgcgctt taccggaaac
     5701 gcgttctgca ccgcgaggtt catcgtcggc atcacgaaac cgacgccgag accgatgaag
     5761 atcatgttga cgatcgcctg tccctgcgtc gtgttgatgt tcatccggct ctgcaggaag
     5821 aggccgatcg tcatcgtcga gagaccgaga atcaccgccc acttgtatcg accggtccgg
     5881 gagatgatct ggccaccggt cgtgctggcg acgacgaggc tcagcatcag cggggcgagc
     5941 acgatgccgg agttggtcgc cgagacgccg acgaccgtct gcatgaagag ggggatgtag
     6001 ctgatcgagc cgaacatccc ggcgccaatg aagaagacgg tcggtacggc gatcgcgaag
     6061 gtccggttcc ggaacatctc gagcggaagg atcggatcca cggcacgtga ttcgttccac
     6121 agaaagaaga tcgcgaagac ggctgccagg acgagcatgc cgacgacctg gaccgatccc
     6181 cacgggtact cggtcccggc ccaggagaag gcgagcagga gcggcaccgc gctgccgatg
     6241 atacccaatg ccccgaacca gtcgatcgga aggtggttct tcgcctggat tttcgggagg
     6301 gccgacatca gcacgatggc cgcgagaatg ccaaccggga tgttgatgta gaagacccag
     6361 cgccagccgg ggccatcggt gaagaacccg ccgaggagcg gaccggctac gctggagaac
     6421 ccgaagacgg atccggtcac gccctgccac ttgccgcgct cggccggcgg gaagatgctg
     6481 ccgatgatcg cgaaggcgtt cgtcatgaga atgccgccgg caatgccctg gagacccctg
     6541 aagagaatca actccgtc
//
LOCUS       DASTMD010000738         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2478883, whole genome shotgun sequence.
ACCESSION   DASTMD010000738 DASTMD010000000
VERSION     DASTMD010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2478883"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1373
                     /locus_tag="VFQ54_10960"
     CDS             138..1373
                     /locus_tag="VFQ54_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0165553.1"
                     /translation="MVRTKNKVDSRLRWGEGSIRRLENHDGDVRYQARWSEGEGKHKR
                     WPSKTFYVRGDEAATKALAEDWLLDVARKKAAGIYAPRNQVTVKGLIEDFLERRLKSE
                     KWQEPTYALYRSRAERHIYPTLGSVKVIELDVPRVQRWIDGMTGARSTLVGQLQVLSG
                     ACKMAITLGIIKTNPASGVEVNPTVKPVRRQAWSFAEVVKVLDAVEKDEDPMWHALFR
                     LALSTGLRPGEIVAMQWDQVDFEKNEILVNRGKSVDASGKTVISERTKTGVDRAVALT
                     PLTASALKTWRVEQNQRRLASTTWDERSFVFTSQRGYFLGDLQWRTYQDALADKLEIT
                     RIMLHGLRHTSASLDLHTDRKIVADRLGHSSPEITAKIYTHVSADEHRAASEAMEARI
                     EAARHAPKSPTPIAKTGSN"
     gene            complement(1886..>2461)
                     /locus_tag="VFQ54_10965"
     CDS             complement(1886..>2461)
                     /locus_tag="VFQ54_10965"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU0165554.1"
                     /translation="PDELTIPANTDVTITLTNKGALNHDFSMDVPTAFTSDPVSGGQS
                     TTFTLNLPPGTYDFYCSQPGHKAAGMVGKLTVVDGGTAGETTTSPTPSATGTASSPAG
                     TPFSVSTVDLAFAPKTLTIPANTNVTITITNKGVLQHDFTIDAAKVQSKLLNGGDSDT
                     VTVNLPAGTYEYYCSVPGHKEAGMVGTLTVQ"
BASE COUNT          529 a          741 c          732 g          459 t
ORIGIN      
        1 tctgcgcttg cgattgaccc agaatacgaa ctaatgttct aaagttcaag gcaaaattgg
       61 cgttgccttt gagtcatacg gcgaaccaaa tttggcacgg tgtctccttg tggtatcggt
      121 gcatcggagg tgacacgatg gtccggacga agaacaaggt cgattcccgc ctgcggtggg
      181 gcgagggatc gatccggcgg ctagagaatc acgacggcga cgtgcggtac caggcgaggt
      241 ggtcggaggg cgaaggcaaa cacaaacgct ggccgtcgaa gacgttctac gttcgaggcg
      301 acgaggccgc gacgaaggcg ctcgccgagg actggcttct cgatgtcgcc cgaaagaagg
      361 cggccggcat ctacgcgccg cgcaaccagg tgacggtcaa ggggctcatc gaggacttcc
      421 tcgagcgccg gctgaagagc gagaagtggc aggagccaac ctacgccctc tatcgatcgc
      481 gcgccgaacg tcatatctat cccaccctcg gctcggtgaa ggtcatcgag ctggatgtcc
      541 cacgggtcca acgctggatc gacggaatga ccggagctcg ctccacactc gtggggcaac
      601 tccaggtcct ttcaggagca tgcaagatgg caatcaccct cggcatcatc aagacgaatc
      661 ccgcctcggg cgtcgaagtc aatcccaccg tgaagcccgt gaggcgccag gcatggtcgt
      721 tcgccgaggt cgtaaaggtg ctggacgccg tcgagaagga cgaggatcca atgtggcatg
      781 cgctcttccg gctcgcgctg tcgaccgggt tgcgccctgg ggagatcgtt gcgatgcaat
      841 gggaccaggt cgacttcgag aagaacgaga ttctcgtaaa ccgcggcaag tcagtagacg
      901 cttccgggaa gaccgtaatc agcgagcgca cgaagaccgg tgtcgatcgc gcggtcgccc
      961 tcacgccgtt gaccgcgagc gcactcaaga cctggcgagt cgaacagaat cagcgccgcc
     1021 tcgcgagcac gacctgggac gaacgatcgt tcgtcttcac gagccagcgc gggtacttcc
     1081 tcggtgatct gcagtggcgc acctatcagg acgccctcgc cgacaagctg gagatcaccc
     1141 ggatcatgct ccacggtctt cgccacacgt cggcctcgct cgatctccat accgatcgga
     1201 agatcgtcgc cgatcgcctc ggacacagtt cgccggagat caccgcgaag atctacaccc
     1261 acgtctcggc ggacgaacat cgcgccgcat cggaggcgat ggaggcccga atcgaggcgg
     1321 ctcgacacgc cccgaaatca ccgacaccaa tcgccaaaac cgggtcgaat tgacccctca
     1381 tcgctagttg agaatgagta ttgaaacgca ctcatttcaa catataaatg tcactctcgc
     1441 gaatcattct caactagtaa aaacccagct gcgtaaaatc cgctgcaaag tcacgtcgcg
     1501 acacggcccg acatcgcccg acatccggaa tccccgctat gtaggggtga attatgtcgc
     1561 gaattgtcgc cagatgacgc gaaaaatggc catcacggac acgatctata tggaaagcag
     1621 cctgctgccg gagaacggtt cgctcaagct cgtcgacggc tgctacccgc tccccgagga
     1681 gcccggcctc agcagctggc actgaggtgt tgcgacgaga gccttgtctg cgatcagcaa
     1741 aagggggcga cataaaagcc ccccctactc acgaccgcgt ttccagatat tcatagccgg
     1801 ttaccccatg cacctcatcg cacggggtac gcgttcctca gcgtccgttc gtacggcggg
     1861 acctgcctct gttgaccgcg accggctact gaacagtcaa cgtcccaacc attcccgctt
     1921 ccttgtgacc gggcacggag cagtaatact cataggtccc tgccggcaga ttgacggtta
     1981 cggtgtcgga atcaccgccg ttcagcaact tggattggac ctttgccgca tcgatcgtga
     2041 aatcgtgctg gagcacgccc ttgtttgtga tcgtgatcgt cacattcgta ttggccggga
     2101 tcgtcagggt cttcggggcg aaggcaagat cgaccgtgct aaccgagaac ggcgtgcctg
     2161 ctggcgagga tgcggtcccg gtcgcactcg gtgtcggaga cgtagtcgtc tcacctgcag
     2221 tcccgccatc gaccacggtg agcttgccca ccatgcccgc ggctttatgc ccaggctgcg
     2281 agcaatagaa atcatacgtt cccggcggga ggttgagtgt aaaggtcgtc gattggccac
     2341 cgctgacggg gtcggacgtg aacgcggtcg ggacatccat cgagaaatcg tggttcagtg
     2401 cacccttgtt ggtaagcgtg attgtcacgt cggtatttgc cgggatcgtc aattcatctg
     2461 g
//
LOCUS       DASTMD010000739         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_425368, whole genome shotgun sequence.
ACCESSION   DASTMD010000739 DASTMD010000000
VERSION     DASTMD010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425368"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VFQ54_10970"
     CDS             <1..949
                     /locus_tag="VFQ54_10970"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU0165555.1"
                     /translation="HGIRILPLLLIFALVFGAVLYGSGRVSLPELQSVEDRARALIHP
                     ETIQSYPAIVTDGDVNLRSAASLDADVVEVMQDGEDVIVTGKPVDGFLPVEVDGQKGW
                     VSVDFIGAEQTGDSSQGPDDGDQPQALDVPAAIAQPTDAPIDAGIPVVADTDTDAAIG
                     TDTAVVDQLDAPRPVPTESPRVVALTPDERWIDVDRTTATVTLYVGTIPQATFQGKIG
                     RDPAPDGFYSTAIGTFHVFSMEKGLAPTPFVDDVYMTDWVGFDPVRKNGFHSPVREAD
                     GSIRDPQNPTTMGCVRLGADDAVTLFDFAAIGMRVEIHD"
     gene            1167..>2270
                     /locus_tag="VFQ54_10975"
     CDS             1167..>2270
                     /locus_tag="VFQ54_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0165556.1"
                     /translation="MPPPNRPRDRARDAATMRRVAGTFRPYRWRVYFIFFLTILATVL
                     GLVNPLLTEKLFDDAIGGKDLHLLVEIVSIMAITPIISAVITVWQTYLNAIVGQHVMQ
                     DLRNRLYAHLQRMPLQFFTSTRTGEIQSRLANDVAGIENVLSDTFVQTVRNLSMAIST
                     VIAMFVLSIPLTLISLALVPVIYILSVRVGRMGREINANRQERLASLTAMMEETLSVS
                     GAMLIKVFGRSRQAQERFTTENQRLADLSIQRQMIGVGLMASSMTLFSLAPVLIYLIA
                     GWQLIDSTNPPLSIGTIIAFTSLQSRLIGGWGPVTQLLNLPVQLQTSLAYFDRIFEYL
                     DLPVSIKERPNAEVLPRGSVRGEVRFDDVWFAYQ"
BASE COUNT          387 a          744 c          718 g          421 t
ORIGIN      
        1 tcatggcatc cgcatccttc cgttgctgct gatcttcgcg ctcgtcttcg gtgcggtgct
       61 gtatggaagt ggccgcgttt ccttgccgga acttcaatcg gtcgaggacc gggcccgcgc
      121 gctgattcat cccgagacga tccagagtta tcccgcgatc gtgaccgacg gtgacgtcaa
      181 cctccggagc gccgcgtcgc tggatgcgga tgtcgttgag gtcatgcagg acggcgagga
      241 cgtgatcgtg accggcaagc ccgtcgatgg cttccttccg gtcgaggtcg acggacagaa
      301 gggatgggtc tcggtcgatt tcatcggcgc ggagcaaacg ggcgattcga gtcagggtcc
      361 tgatgacggc gatcagccgc aggcgctcga tgtcccggcg gcgatcgcgc agccgacgga
      421 tgctccgatc gatgccggga taccggttgt cgcagacacg gacacggacg ccgcgatcgg
      481 aacggatacg gcggtcgtcg accagctgga cgcgccgcgt ccggtgccga cggagagccc
      541 gagggtcgtg gcgctgaccc cggacgagcg gtggatcgac gtcgaccgca cgacggcgac
      601 ggtgacgctc tacgtgggga caataccgca ggcgaccttc caggggaaga tcggtcgcga
      661 tccggcaccg gacggcttct actccacggc gatcggcacc ttccacgtct tcagcatgga
      721 gaagggcctc gcgccgacac cgttcgtcga tgacgtctac atgacggatt gggtcggctt
      781 cgatccggtc cggaagaacg gctttcactc gccggtccgg gaagctgatg gatcgatccg
      841 cgatccccag aacccgacga cgatgggctg tgtccgcctc ggcgccgacg atgccgtgac
      901 gctcttcgat ttcgccgcga tcggcatgcg cgtcgaaatt cacgactgag cccgggtgcg
      961 tccgacggcg tgcgactcgc aaccggcatc cacgcggggt atcttgctcg taacaaacgt
     1021 ggcgatgatg cgcgttcacc tggttggacg ggttgaggtc gatcggaacc agaagcggtg
     1081 gagccccagg gcatgccgcg aggacgatcg gtacccgcgt cgtcatgcat tcggagcacg
     1141 tcaccatgcg agaaatgatg cgggggatgc cgcccccgaa ccgaccccgt gaccgcgccc
     1201 gcgacgccgc cacgatgcga cgcgtcgccg ggaccttccg tccctaccgc tggcgcgtct
     1261 acttcatctt cttcctgacg atcctcgcga cggtcctcgg cctcgtcaac ccgctgctta
     1321 ccgagaagct cttcgacgac gcgatcggtg gcaaggatct ccacctgctc gtcgagatcg
     1381 tctcgatcat ggcgatcacg ccgatcatca gcgccgtgat caccgtctgg cagacgtacc
     1441 tgaacgccat cgtcggccag cacgtgatgc aggacctgcg gaacaggctc tacgcgcatc
     1501 tccagcggat gccgctccag ttcttcacgt cgacgcgcac cggagaaatc cagtcccggc
     1561 tcgcgaacga tgtcgccggg atcgagaacg ttctctccga taccttcgtg cagacggtgc
     1621 gcaacctctc gatggcgatc agcaccgtga tcgcgatgtt cgtgctcagc atcccgctga
     1681 cgctgatctc gctcgccctc gttccggtga tctacatcct cagcgtccgc gtcggccgga
     1741 tgggacgcga gatcaacgcc aatcgccagg aacggctcgc ctcgctgacc gcgatgatgg
     1801 aggagacgct ctccgtcagc ggtgcgatgc tgatcaaggt gttcgggcgg tcgcgacagg
     1861 cgcaggagcg cttcacgacg gagaaccagc gcctcgccga tctcagcatc cagcgccaga
     1921 tgatcggtgt cggcctgatg gcctcatcga tgacgctctt ctcgctggcg ccggtcctga
     1981 tctacctgat cgccggttgg cagctcatcg actccaccaa ccctccgctt tcgatcggca
     2041 cgatcatcgc cttcacgtcg cttcagagca ggctgatcgg tggttggggt cccgtgacac
     2101 agctgctcaa cctgccggtc cagctgcaga cgtcgctcgc ctacttcgat cgcatcttcg
     2161 aatatctcga cctgccggtc agcatcaagg agcgtccgaa cgctgaagtc ctgccgcgtg
     2221 gcagcgtccg cggcgaggtg cggttcgacg atgtctggtt cgcctatcag
//
LOCUS       DASTMD010000740         5525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2125134, whole genome shotgun sequence.
ACCESSION   DASTMD010000740 DASTMD010000000
VERSION     DASTMD010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125134"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..687)
                     /locus_tag="VFQ54_10980"
     CDS             complement(13..687)
                     /locus_tag="VFQ54_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU0165557.1"
                     /translation="MSTVPSQSSTSDDALLELRNVTTIFGQGSGATVASDRVNLRIDL
                     SPPRIVSIVGESGSGKTTAARSLLGLQPTTHGEALWRGKNIYDLHRHDRMTFRREVQV
                     IFQDPYGIFNPFYPINRVFNMAIKKFKLAKNKGERDQLIEEALKAVNLRPPDVLGRYP
                     HQLSGGERQRVMLARAYLLKPKVIVADEAISMLDVAVRAVIMNILIDFKEKLGISTMP
                     RTIERW"
     gene            complement(684..1799)
                     /locus_tag="VFQ54_10985"
     CDS             complement(684..1799)
                     /locus_tag="VFQ54_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0165558.1"
                     /translation="MATTMTNSNPATGTSPGPGSNQPATQIPLLEIRNLDVHFNTARG
                     VARVVDNCNLVVHRNEIVGIAGESGSGKTTLVEAILQIIRFTNRVTRGQVLFSPDGGE
                     PVDLLTLGEKEMRKLRGSKLSYVPQGSINSLNPIAKIGDQIVEGMVDHGMSERASKAK
                     VPDLLLKVGLDPGVARMYPHELSGGMKQRVIIAIAIAMEPALIVADEPTTALDVNVQR
                     LILETLHHLRDELNVAIMIVSHDLPVHAQLVERIGIMYAGQIVESGDIRSTVKLPLHP
                     YTFGLMNAIPSIGGSRERLQGIGGSTPSPNAWPDGCRFHNRCPRAMDICRSTPPKLAE
                     VEAGPRMAAAQEMIDVIPGRLVACHLYPESTPEGSRS"
     gene            complement(1842..2717)
                     /locus_tag="VFQ54_10990"
     CDS             complement(1842..2717)
                     /locus_tag="VFQ54_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165559.1"
                     /translation="MAVLRLWRSSGKFRFGFTIIFLLIAVALLHQPIIHWRLGKDAFG
                     DYKDPMTAGLYPIFLSPSSAHWLGTDRYGRDVFALVMLALPTSLWVAFIAGAVSTVIG
                     AVVGFTAGYRGGTTDSVLRTITDMFIVIPTLPLIIILSRYSDHVTSLQLALILAIFSW
                     PFAARVIRTQVLSLRERPYIELSKMTNMSDREIIVGDIFPNMAPYVAIGFANASVGSA
                     LALVGLTIIGMGPSNQMDLGRMIADALGWGAISLHKEAIFIAPSVLLILLFVGLAWMG
                     QGLEETFNPRLRRPS"
     gene            complement(2736..3833)
                     /locus_tag="VFQ54_10995"
     CDS             complement(2736..3833)
                     /locus_tag="VFQ54_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165560.1"
                     /translation="MASTTPNAVANALPDAVEAVEATPKQARISPTAQYYLRRIGLYI
                     ITLWGSLSATFFFFRLLPGDPISGIIAQMQARGLQSQQTSSADMVAYYKRAFGLDGNL
                     WEQYIRYFNQVIFHLNFGPSVLSYPKPAMDLVARGFPWTMAIIGTSVILSWILGMAIG
                     TLVGWKRNSRISNWITNFCLLFQQINAYFIALILVIFISYRWNLLPPNGAFDNSLQPA
                     WSLKFIGSAIHYGILPVMAQVITGTAGWVIGTRALIVTLLGEDFLTYANAKGLSTRRI
                     LFRYVLRNSWLPQIAALGISLGFVVNGNILIEQLFRYPGVGSLLINSVNVKDVNTAMA
                     IVSVLIFFVLTINLIIDLILPVFDPRVKYAK"
     gene            complement(4094..>5525)
                     /locus_tag="VFQ54_11000"
     CDS             complement(4094..>5525)
                     /locus_tag="VFQ54_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0165561.1"
                     /translation="DFTQSTLSINPAAKWNDGTPFTADDVVFSMGLYKANPNFSNASY
                     YNGVVKSVTAKDATTVVFELTSAQPRFHYNYYAGIIEDNLRIVPKHIWEKEDANTFTF
                     NPPVYTGPYTLKESSSSKLYYLWQKNPDYWNKATIDPAPNYVAYVQQTAMDAAVQAFT
                     AGNVDVTSADYPNQQVIASSYKDQTSYEFPDPCPRGIWFNAESPSGLFKTAEGRWALS
                     YLLDRETIAKTIWQPETVAATYPWANYPGWTKWAPDSVMGKFDLTFNPQKAEEMLDAL
                     GAKKNGDKRQLNGKDLSLTIITPVLTSQPEYQIAASFAQALQKVGIGADVKSLPGSAF
                     GDSFQLGEFDITSHWICGMQFDPDQLFTNFNSKYYVANGTRQTFGQDASNIRFKSPAL
                     DKLINEMDAVNPDDPKNMQLFLDGLTQFETDLPCVPTIQTIYAMMYNTKYWTNWPDDT
                     KPTTIPANWWGQFLLTIANLKATGAS"
BASE COUNT         1077 a         1611 c         1746 g         1091 t
ORIGIN      
        1 gacgggaaca acttaccacc tttctatagt ccttggcatc gtcgagatgc caagcttttc
       61 cttgaagtcg atcaggatgt tcatgatcac ggcccgaacg gcgacgtcca gcatcgagat
      121 cgcctcgtcg gcaacgatca ccttcggctt caggagatag gcacgagcga gcatcacgcg
      181 ttgacgctca ccaccgctca actggtgcgg atagcgaccg agcacatcag gcgggcgcag
      241 attgacggcc ttgagcgcct cctcgatcag ctggtcccgc tctcccttgt tcttcgccag
      301 cttgaacttc ttgatcgcca tgttgaagac gcgattgatc gggtagaacg gattgaagat
      361 gccgtaggga tcctggaaga tcacctgcac ttcgcggcgg aacgtcatcc ggtcatggcg
      421 atgcaaatca tagatattct tgcctcgcca aagggcctcg ccatgcgtcg tcggctgcag
      481 gccgagaaga gaccgcgccg ctgtcgtctt gcccgagcca ctctcgccga cgatgctcac
      541 gatacgtggc ggcgaaaggt cgatgcgaag attgacgcgg tcgctggcga cggtcgcgcc
      601 ggatccttgt ccaaagatcg tcgtgacgtt tcgcaactcg aggagcgcgt cgtcggaagt
      661 cgaggattgg gacggaacgg tgctcacgag cggctccctt cgggcgtcga ttccggatag
      721 agatggcagg cgacgagtcg tccgggaatc acgtcgatca tttcctgagc ggcggccatt
      781 cgtggtcccg cctcgacctc ggcgagcttc ggtggcgtag accggcagat atccatcgcc
      841 cgcgggcacc ggttgtggaa ccggcagcca tccggccagg cattcggact gggcgtcgag
      901 cccccgatgc cctgcaggcg ttcgcgcgag ccgccgatcg acgggatcgc gttcatcagt
      961 ccgaatgtat atggatgaag cggaagcttc accgtcgaac ggatatcccc gctctccacg
     1021 atctggcccg cgtacatgat gccgatccgc tcgaccagct gcgcgtgaac ggggaggtcg
     1081 tgactcacga tcatgatcgc gacattcagt tcatcgcgga ggtgatgcag cgtctcgaga
     1141 atcaggcgct gcacgttcac atccagcgcg gtcgtcggct cgtcggcgac gatcagggcc
     1201 ggctccatcg cgatcgcgat cgcgatgatc acgcgctgct tcattccgcc gctgagttcg
     1261 tgcggataca tcctggcgac gccggggtcg agaccgacct tgagcagaag atccgggacc
     1321 ttggccttcg atgcccgctc ggacatccca tgatcgacca tgccctcgac gatctgatcg
     1381 ccgatcttgg caatcgggtt cagggagtta atcgatccct gcggcacgta ggaaagcttg
     1441 ctcccgcgga gcttccgcat ctccttctcg ccgagcgtga gcaggtcgac gggctccccg
     1501 ccatctggcg agaagagaac ctgcccccgc gtgacgcggt tcgtgaaccg gatgatctgg
     1561 aggatcgcct cgacgagcgt cgtctttccg gatcccgact cgccggcgat accgacgatc
     1621 tcattgcggt gtactacgag gttgcagttg tcgaccaccc gtgcgacacc gcgcgcggtg
     1681 ttgaagtgca catcgaggtt gcgaatctca agcagtggaa tctgcgtcgc cggctggttc
     1741 gagccaggcc cgggtgacgt tcccgttgct ggattggagt tcgtcatcgt cgttgccata
     1801 cgcgttccac tttcacatgc gagacggaag ccgccggtac cctacgacgg gcgacgcagg
     1861 cgggggttga aggtctcctc aagtccctgg cccatccagg cgagtccaac aaacagaagg
     1921 atcagcagca cgctcggcgc gatgaagatc gcttccttgt ggaggctgat ggcgccccac
     1981 ccgagggcgt cggcgatcat ccgccccaga tccatctggt tgctcgggcc cataccgata
     2041 atcgtcagac caacgagcgc aagcgccgag ccaacggagg cattggcgaa accgatcgcg
     2101 acatacggcg ccatgttcgg gaagatgtca ccgacgatga tctcccggtc gctcatgttg
     2161 gtcatcttcg agagttcgat atagggccgc tcgcgaagac tgagcacctg ggtacgaatc
     2221 acgcgggccg caaatggcca gctgaagatc gcgagaatca gcgccagctg caggctggtg
     2281 acgtggtccg agtagcggga gaggatgatg atcaatggaa gggtcgggat gacgatgaac
     2341 atgtcggtga tcgtgcggag caccgagtct gtcgtgcccc cgcgatagcc cgccgtgaag
     2401 ccaaccacgg cgccgatcac cgtcgagaca gccccggcga tgaacgccac ccagagcgag
     2461 gtgggcaatg ccagcatcac caacgcaaag acgtcacgcc cgtagcgatc ggtcccgagc
     2521 cagtgcgcgc tgctcggact caggaaaatc ggatacaacc ccgccgtcat cggatccttg
     2581 taatccccga aggcgtcctt gcccagccgc cagtgaatga tcggctggtg gagaagcgcg
     2641 acggcgatga gcaggaagat gatcgtgaag ccgaaacgga acttgccgct gcttcgccag
     2701 agtcgaagaa ctgccatgac ggaacctgac ctttactact tggcgtactt cacgcgcgga
     2761 tcgaagaccg gcaggatcag gtcgatgatc aggttgatcg tcaacacgaa gaagatcagc
     2821 acgctcacga tcgccatcgc cgtgttgaca tccttgacgt tcaccgagtt gatcagaagc
     2881 gaaccaacgc ccggatagcg gaagagctgc tcgatcagga tatttccgtt gacgacgaag
     2941 ccgagcgaga taccgagggc cgcgatctgc gggagccagg agttacgcag gacgtagcgg
     3001 aacagaatgc gccgcgtgga gagacccttc gcattcgcat aggtgaggaa gtcctcaccg
     3061 aggagcgtca cgatcaatgc gcgcgtcccg atcacccagc cggcggtacc ggtgatcacc
     3121 tgtgccatca ccggcaggat cccgtagtga atcgcgctgc cgatgaactt gagcgaccat
     3181 gccggctgga gcgagttgtc gaaggcgccg tttggcggca ggagattcca tcgataggag
     3241 atgaagatca cgagaatgag ggctatgaag tacgcattga tctgctggaa gaggaggcag
     3301 aaattcgtga tccagttgga gatgcggctg ttccgcttcc acccgacgag cgtcccgatc
     3361 gccatcccga ggatccagga gaggatcacc gacgttccga tgatcgccat cgtccagggg
     3421 aaaccacgcg cgacaagatc cattgccggt ttcgggtagc tcaataccga gggtccgaag
     3481 ttcagatgga agatgacctg attgaagtac cggatgtact gctcccacag gttgccatcg
     3541 agaccgaatg cacgcttgta gtacgcgacc atatccgccg acgacgtctg ctgggattgg
     3601 agtcctcgcg cctgcatctg ggcgatgatt ccggaaatgg gatcgcctgg cagcaatcgg
     3661 aaaaagaaga atgtagcgga aagcgaaccc cagagggtga tgatatagag cccgatgcgg
     3721 cggagataat attgcgccgt aggagagatg cgcgcctgct ttggcgtcgc ctcaacggcc
     3781 tcgaccgcgt cgggaagtgc gttggcaact gcattagggg tggtggaggc catgatcgtg
     3841 ctcccgggtt ggtatggaac cttgctatgg cgtcgtcgtc gtggatcggc gagacgcatt
     3901 ccctggcata tgctgctttg cgccagggag accccgatca ttcggcggct tccgtgccgc
     3961 ggctcagggc caagggcgtt ctgagccgga tcaggtactg atacgaaacg tcatcaggag
     4021 atgaccggct tcggcgccgg tcatctcctg gtttgccggg cgcgagatca ctcgcgcccg
     4081 gcggtgcgtc aacctacgat gccccggtgg ccttcaggtt ggcgatcgtc agcaggaact
     4141 gaccccacca gttggccggg atcgtggtcg gcttggtgtc gtccggccag ttcgtccagt
     4201 acttggtgtt gtacatcatc gcgtagatcg tctggatggt cggcacgcac ggcagatcgg
     4261 tctcgaactg cgtgagcccg tcgaggaaga gctgcatgtt cttcggatcg tccggattca
     4321 cagcatccat ctcgttgatc agcttgtcga gcgccgggct cttgaagcgg atgttgctcg
     4381 catcctgacc gaatgtctgg cgagttccat tcgcgacgta gtacttcgag ttgaagttgg
     4441 tgaacagctg atcgggatcg aactgcatgc cgcagatcca gtggctcgtg atgtcgaatt
     4501 cgccgagctg gaacgagtcg ccgaaggcgg atcccgggag gctcttgacg tccgcgccga
     4561 tgccgacctt ctggagtgcc tgagcgaacg aagccgcgat ctgatactcc ggctggctcg
     4621 tcagcaccgg ggtgatgatc gtcaggctga gatccttgcc gttcagctgg cgcttgtcgc
     4681 cattcttctt ggcgccgagg gcatcgagca tctcctccgc cttctgcgga ttgaaggtca
     4741 ggtcgaactt gcccatcacg ctgtctggcg cccacttcgt ccatccgggg tagttcgccc
     4801 acggataggt cgcggccacg gtctccggct gccagatcgt cttcgcgatc gtctcgcggt
     4861 cgagcaggta ggagagcgcc cagcgtccct cggccgtctt gaagaggcca ctcggggact
     4921 cggcattgaa ccagatgcca cgcgggcacg gatccgggaa ctcgtaggag gtctgatcct
     4981 tgtacgaact cgcgatcacc tgctggttcg gatagtcggc actcgtcaca tcgacgttgc
     5041 cggccgtgaa tgcctggacc gccgcgtcca tcgccgtctg ctggacgtag gcgacgtagt
     5101 tcggcgccgg atcgatcgtg gccttgttcc agtaatccgg gttcttctgc cacaggtagt
     5161 acagcttgct gctggacgat tccttcagcg tgtacgggcc ggtgtagacg ggagggttga
     5221 aggtgaacgt gttggcgtct tccttctccc agatatgctt gggcacgatg cgcaggttgt
     5281 cttcgatgat gcctgcgtag tagttgtagt ggaagcgagg ctgggcgctg gtgagctcga
     5341 agacgaccgt cgtcgcatcc ttggccgtca ccgacttcac cacgccgttg taatacgacg
     5401 cgttactgaa gttcgggttg gccttgtaaa gtcccatcga gaagacgacg tcatccgcgg
     5461 tgaacggcgt tccgtcgttc cactttgccg ccgggttgat ggagagtgtg gactgcgtga
     5521 agtcg
//
LOCUS       DASTMD010000741         1837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3195710, whole genome shotgun sequence.
ACCESSION   DASTMD010000741 DASTMD010000000
VERSION     DASTMD010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3195710"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..1566)
                     /locus_tag="VFQ54_11005"
     CDS             complement(334..1566)
                     /locus_tag="VFQ54_11005"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165562.1"
                     /translation="MRQVLANRSFVFLWSAGLFAQLAWWMLHTSMLVLVFERTGSAFA
                     TGLIPVFSSLPTILLGPIAGRIVDRWDRKRVMQCGSLTLAGLMAVSLPIANDAPVAFL
                     DAFIVVQSVVMTVMTPAENSLLPTLVEADQLKTANALNVLNDGLGRIVGPAIGSLILV
                     QLGFAATVAVCLVIFVAAWGFLVGMGTPTEARQRHGAAWGAPFREQFALTTRVLAERG
                     VLAAVVIVFALYMVADVPLSAISSAFFSESLHASPEEFGFMLSLRGVAGILGGLLILV
                     VSRWLSEKTLLLGGLLMYGFAIAYLGVVNTYGIGLLGLILVGPAAAAIQTGMNSLLQK
                     YTPPDISGRIFALVGSIGATIALVVSITAGSVAEVTGTRAVVIASGCLQILPALVALR
                     RLPSASGSTRRATRNAMP"
BASE COUNT          350 a          598 c          629 g          260 t
ORIGIN      
        1 cagcggcggt cccgccctac gaacgaacgc ggtgcgaacg gcgtcgtttc cgtggcgggt
       61 cacccggtgt gatccatcga ccgcaggtcg atatgatcgt tgcatgcgcg ttgtcgttgt
      121 gcgcagatcc ctccccggaa ccgtagacgc ggcccttgtg gccgccccgg gtcggctccg
      181 ccgatccgtc ttccgggagc gatatgcatg gcgcggcgat ctgttcggag cttcccgttt
      241 gcggccagag ataaactcag gccctacaaa ccccgttgaa cgaaagcccg cgttgatggc
      301 gggattcgtc tccagccctc gttcattccg catctacggc atcgcgttcc tggtcgcgcg
      361 gcgcgtcgat cccgacgccg acgggaggcg ccgcagcgcg acgagcgccg gcaggatctg
      421 gaggcacccg gaggcgatga cgaccgcccg cgtgcccgtc acctcggcga cgcttcccgc
      481 cgtgatgctc acgacgagcg cgatcgtggc gccgatcgag ccgacgaggg cgaagatgcg
      541 tccgctgata tccggcggcg tgtacttctg caggagcgag ttcatccccg tctggatcgc
      601 cgccgcggcc ggtccgacga gaatcagccc gagcaggccg atcccatacg tgttgacgac
      661 acccaggtag gcgatcgcga acccgtacat cagcagaccg ccgaggagga gcgtcttctc
      721 cgagagccac cggctgacga cgaggatcag gagaccgccg agaatcccgg cgacaccgcg
      781 caacgagagc atgaatccga attcctcggg actcgcgtgg agcgattcac tgaagaacgc
      841 cgacgagatg gcggagagcg ggacgtccgc caccatgtag agcgcgaaca cgatcacgac
      901 cgccgcgagc accccgcgct cggcgaggac gcgcgtcgtg agggcgaatt gctcccggaa
      961 cggcgctccc cacgcggcac cgtgccgctg tcgtgcctcg gtcggtgtcc ccatcccgac
     1021 caggaatccc caggcggcga caaagatgac gaggcagacg gccacggtgg ccgcgaatcc
     1081 cagttggacg aggatcagag agccgatcgc gggaccgacg atccgcccga gaccgtcgtt
     1141 gaggacgttg agcgcattgg cggtcttcag ctgatccgcc tccaccaggg tcggcaggag
     1201 ggaattctcc gccggcgtca tcaccgtcat cacgaccgac tggacgacga tgaaggcatc
     1261 gaggaaggcg accggggcgt cgttcgcgat cggtagcgag acggccatca gcccggcgag
     1321 cgtgagggag ccacactgca tcacccgctt gcgatcccag cgatcgacga tgcgaccggc
     1381 gatcgggccg aggaggatgg tgggcagcga cgagaagacg gggatcagac cggtcgcgaa
     1441 ggcggatccg gtccgctcga agacgaggac gagcatggag gtgtgaagca tccaccaggc
     1501 gagctgcgcg aacagccccg ccgaccagag aaagacgaat gaccggttcg cgagaacctg
     1561 ccgcatgagg gactcccttt cggctccgga gcgcgcgatt cgcacgaccg gaaaccagcg
     1621 cgtgatgatc cagggcgtca cccaggggat gacaccgcgt tcacatgacg tcttgggagc
     1681 tcgaaggggg aggaaacggc aacgcggcga cgatcgtcag tggagatcgt cgggcgtcac
     1741 ttccgagtgc gggtcaggtc accgtcacag ccgccagaga atcgggatgg tctcccgaat
     1801 cagagctccc tggcggcgcg ccggccggct ggtgccg
//
LOCUS       DASTMD010000742         1836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2569858, whole genome shotgun sequence.
ACCESSION   DASTMD010000742 DASTMD010000000
VERSION     DASTMD010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2569858"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFQ54_11010"
     CDS             <1..315
                     /locus_tag="VFQ54_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165563.1"
                     /translation="KTETRNTFQLEALETTYFNGLDGRRVSQVLTDAQGLWTELVFSG
                     DAIYLLPADGCRDELGGLFADPRSGWDDDDQDRPAGAKIFPNELDWKDIDGQTYLRAW
                     WD"
     gene            315..542
                     /locus_tag="VFQ54_11015"
     CDS             315..542
                     /locus_tag="VFQ54_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165564.1"
                     /translation="MSDRIKLPLLPALHIEIPVDTSTDISFEERGREGIAVWFTPPRP
                     IERGDVFFAPVPRDKVDAMRDWMTAWLEATK"
     gene            539..1003
                     /locus_tag="VFQ54_11020"
     CDS             539..1003
                     /locus_tag="VFQ54_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165565.1"
                     /translation="MRPPLVIKVPGTIPYSLTANGRRSTHWRTIHRDAEDLKTRTHYA
                     ALEAIGDPNDPYLCFQHDPWPLAITYVVGWEKRRHLMDDDNVIAAMKNVRDTIASVLG
                     VDDKHFLTNGLMQERDPEGKGYIGITIISSAAYVQVPRDWYERAVDAIGDVA"
     gene            1000..1143
                     /locus_tag="VFQ54_11025"
     CDS             1000..1143
                     /locus_tag="VFQ54_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165566.1"
                     /translation="MILIRSLLTNDLRLMVPRDYRPRKYDVYVDRERGDWWTVVDADV
                     REW"
     gene            1143..1430
                     /locus_tag="VFQ54_11030"
     CDS             1143..1430
                     /locus_tag="VFQ54_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165567.1"
                     /translation="MEEEELTESFTNDSHTMLQATSEVLAFFEPRIRAEERRAVIEEL
                     AREAEAGFYDEDLIFEHADWSLSKQEGHQVAAWLRSHANCGESATIGEEEG"
     gene            1430..1831
                     /locus_tag="VFQ54_11035"
     CDS             1430..1831
                     /locus_tag="VFQ54_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165568.1"
                     /translation="MVILIAILAGIWIVLAIVTAIVTIRRDKTAPDPESFDIAVRTLG
                     HAMIDPFVPAIERFNNRLERMLRILHWPGARRCPPLNTARPPRRSWMTPRCSTVASIY
                     PGDDHEDHYRTTRMGPRSLDRAGWPTVDHHE"
BASE COUNT          390 a          529 c          590 g          327 t
ORIGIN      
        1 aaaaccgaga cacgaaacac gttccagttg gaagcgctcg aaacgacgta cttcaacggc
       61 cttgacgggc ggcgggtcag ccaagtgcta accgatgcac aaggtctctg gaccgagctc
      121 gtctttagtg gcgatgccat ctacctgctt ccggctgatg ggtgtcgcga cgaactcggt
      181 ggactcttcg cggacccccg atccggctgg gatgatgacg atcaggatcg tccagccgga
      241 gcgaagatct ttccgaacga gctggactgg aaagacatcg acggccagac gtatctccgg
      301 gcatggtggg actgatgagc gacaggatca agctgcccct gcttccggcg ctccacatcg
      361 agatcccggt cgacacgtcg acggacatct cgttcgagga acgagggcgt gaggggatcg
      421 cggtctggtt cacgccgccc cggcccatcg aacgcgggga cgtcttcttc gcgccggtcc
      481 cccgcgacaa ggtggacgcg atgcgggact ggatgacggc gtggctggag gcaacgaagt
      541 gaggccacca ctggtgatca aggtgcctgg caccattccg tattcactga cggccaacgg
      601 acgccggtcg acccattggc gcacgatcca ccgggacgcc gaggacctca agacgcgtac
      661 ccattacgcg gcactggagg cgataggtga tcccaatgac ccatacctct gcttccagca
      721 cgatccgtgg ccgctggcca tcacctacgt ggtgggctgg gagaagaggc ggcacctgat
      781 ggatgacgac aacgtcatcg ctgccatgaa gaacgtgcgg gataccattg ccagtgtgct
      841 gggggtcgac gacaagcact tcctgacaaa cggcctgatg caggaacgcg atccggaagg
      901 gaagggctac atcggcatca cgatcatcag ctctgccgcc tatgtgcagg tgcctcggga
      961 ctggtacgag cgagccgtcg acgcgatcgg agatgtcgca tgatcctcat ccgcagtctc
     1021 ctgacgaacg acctgcgcct gatggtgccg cgcgactatc ggcctcggaa gtacgacgtg
     1081 tacgttgacc gggagcgcgg cgactggtgg accgtggtgg acgcggatgt aagggagtgg
     1141 tgatggaaga ggaagaactg acagaaagct ttacgaacga ctcgcacacc atgctgcagg
     1201 ctacgtccga ggttcttgcg ttctttgaac ccaggattcg tgcagaagag cgccgcgccg
     1261 tgatcgaaga gctggcgcgc gaggcggagg caggattcta tgacgaggat ctgatcttcg
     1321 agcatgcaga ctggtccctg tcaaagcagg aggggcacca ggtcgccgcg tggctgcggt
     1381 cgcatgccaa ttgtggcgaa tccgccacaa ttggagagga ggaggggtga tggtgatact
     1441 catcgcgatt cttgccggca tttggatcgt cctcgcgatc gtcactgcga tcgtcacgat
     1501 tcgacgggac aagactgcgc ccgaccctga atcgtttgac atcgccgtgc gcaccctcgg
     1561 acatgccatg atcgatccgt ttgtcccggc gatcgagcgg ttcaataatc ggcttgagcg
     1621 gatgcttcga attctccact ggcccggagc aagacgatgc cccccgctga acaccgcaag
     1681 gccgccaagg cgctcatgga tgacgccgag gtgctcaacg gtcgcgtcga tttacccagg
     1741 agatgatcat gaagaccatt accgaacgac cagaatggga ccgcgtagcc tcgatcgtgc
     1801 aggatggccg acggttgacc atcacgagtg aaagcg
//
LOCUS       DASTMD010000743         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2837338, whole genome shotgun sequence.
ACCESSION   DASTMD010000743 DASTMD010000000
VERSION     DASTMD010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2837338"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..635
                     /locus_tag="VFQ54_11040"
     CDS             276..635
                     /locus_tag="VFQ54_11040"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0165569.1"
                     /translation="MFIRHQDKSQGTVDASAMFTGVVSRQELVREGDAPDLRVTGVSF
                     EDGARNKWHSHTTEQVLVVTDGEGIIATDTEERPIVPGDVVLIQAGERHWHGAAPGKN
                     MTHLAILLPGEMHVYDE"
     gene            complement(843..>2730)
                     /locus_tag="VFQ54_11045"
     CDS             complement(843..>2730)
                     /locus_tag="VFQ54_11045"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEU0165570.1"
                     /translation="IVAQSPNTNNGDGSRNLRGSVKSEMETWRASMSIVDAVSTAPNP
                     QFARENRIDLTGEWHFAHDDADVGLRERWFAHPERIDRRIVVPYPPESRLSGIHDTNF
                     HRVLWYARPLSVETAESGAHPLLHFGAVDYEASVWVDGNWVGSHTGGQTPFHFDIADA
                     LDPEVTEHWIVVRATDDPLDDEQPRGKQDWKESPHVIWYHRTSGIWQPVWLEHAPATH
                     IESVHWKFDAATWRVDFEVTLSGRAVDGTMLRIDLEHEAVKLPSIATAVHGNRASGQI
                     GLATSHETMNSGQLMWSPSRPNLIGARLTLTTPDQPDDEVLTYLGLRTVETSPNRIRI
                     NGQPIFLRLVLNQGYWPESQLTAPSPEALKREVELILELGFNGARNHQKVEDPRFHYW
                     ADRLGLLVWGEMPAAFIYTDKAIGRHLAEWRDAVVRDRNHPSVIAWVPFNESWGVSEA
                     GHSVAQQHAVKAAYHTAHQLDGTRPVIGNDGWDQVAGDIFTIHDYTWDPESLARRYSS
                     PEAVEEALDEHYPGSRRLVAGDYDHVGKPVVVSEFGGVSYAPASGANWFGYGTVGTEE
                     EYLAKYEELTSRLHSFPKLAGFCYTQLTDTEQEINGLLTEDRVPKVDINEIRRITRGP
                     DRSR"
BASE COUNT          482 a          851 c          886 g          511 t
ORIGIN      
        1 gcgggcgccc acgcttgcga tgcggtccaa taccccgcga ggatcgggac cggcctcaca
       61 gagatccccg gcgacgatga tctcatcgac accttcgcgc tcgatatccg cgagaacgga
      121 atccaaggcg agactgaacc cgtggatgtc tgatatcact gcgatcttca tgtttttcct
      181 gacacctgcg cctttccgct ggatgcggag cgtgtgcgac cattcaagca cgaacctcgt
      241 caaccagcca cgtatgtatc aagaatggag ccgccatgtt catccggcat caggacaagt
      301 cacagggaac ggtcgacgcg agcgcgatgt tcaccggcgt cgtctcgcgc caggagctcg
      361 tgcgtgaagg cgacgcaccg gatctccgcg tcacgggggt gagcttcgag gatggcgcgc
      421 gcaacaagtg gcacagccac accaccgagc aggtattggt cgtcacggac ggcgagggaa
      481 tcatcgcgac ggatacggag gaacggccga tcgtgccggg cgatgtcgta ctgatccagg
      541 caggggaacg tcactggcat ggcgcagccc cggggaagaa catgacgcac ctcgcgatcc
      601 tgcttccggg cgagatgcac gtctacgacg agtaacggcg ccgaccagcc gatgccgtcc
      661 ctcacctggc ccggcatcgg ctggcccgcc gcgcgtacga tcgcgttttt ccattttcgc
      721 atcatctgaa tcaccgggga tgcgatgatt gtcgacgttg ctgcagccgc tcagacggcg
      781 aacgccagca tgcgtgcgta cggggggact tctatgtccc cccttgggtt gaggacgcca
      841 gcctaccggg atcgatccgg acccctggtg atccggcgga tctcattgat gtcgacctta
      901 ggtacgcggt cctcggtcag gagaccgttg atctcctgct cggtatcggt cagctgcgtg
      961 tagcagaagc cagcgagctt ggggaagcta tggagccggg aggtcagctc ttcgtatttg
     1021 gcaaggtatt cctcctcggt gccaacggtg ccgtagccga accagttcgc gccggaggcc
     1081 ggggcgtagc tgacaccgcc gaactcgctg acgacgaccg gcttcccgac gtgatcgtaa
     1141 tcaccggcga cgagccgccg cgaaccggga tagtgctcat cgagcgcctc ctcgaccgct
     1201 tccggcgaac tgtaacgacg cgcgagggac tccggatccc aagtgtagtc gtggatcgtg
     1261 aagatgtccc cagcgacctg atcccaaccg tcgttgccga tcaccggtcg cgtgccatcg
     1321 agctggtgcg ccgtgtgata ggccgccttg accgcatgct gctgggcgac ggaatggccc
     1381 gcctcactca caccccagct ctcgttgaat ggcacccatg cgatcaccga aggatgattg
     1441 cgatcccgga cgacagcatc ccgccattcc gcgaggtggc gaccgatggc cttgtccgtg
     1501 tagatgaacg ccgccggcat ctctccccag accagcaggc cgaggcggtc cgcccagtag
     1561 tggaagcgcg ggtcctcgac cttctgatgg tttcgggcgc cattgaagcc cagttccagg
     1621 atcagttcga cctcgcgctt cagcgcctcc ggggaaggtg ccgtcagctg gctttccggc
     1681 cagtagccct ggttgagcac gagacggaga aagatcggct gtccgttgat ccggatccgg
     1741 tttggcgagg tttcgacggt gcgaagtccg aggtaggtga ggacctcatc gtcgggctgg
     1801 tccggcgtcg tcagcgtcag gcgggcgccg atcaggttcg gacggctcgg cgaccacatg
     1861 agctggcccg agttcatcgt ctcgtgactc gtcgcgaggc cgatctggcc ggaggcgcga
     1921 tttccgtgga cggccgtcgc gatcgagggc agcttgacgg cctcgtgttc gagatcgatc
     1981 cgcagcatcg tgccgtcgac ggcgcggccc gagagcgtca cctcgaaatc gacacgccat
     2041 gtcgcggcgt cgaacttcca gtggacggac tcgatgtgcg tcgcgggggc gtgttcgagc
     2101 cagaccggct gccagatgcc cgacgtccgg tgataccaga tgacgtgtgg cgattccttc
     2161 cagtcctgct tgccccgtgg ctgctcgtcg tcgagaggat cgtccgtcgc acggacgacg
     2221 atccagtgct cggtcacctc tggatcgagt gcgtccgcga tgtcgaagtg gaacggtgtc
     2281 tgaccgccgg tatgcgagcc gacccagtta ccatcgaccc agacagatgc ctcgtagtcg
     2341 accgcgccga agtgaaggag cggatgcgcg ccggattctg cggtttcgac cgaaaggggc
     2401 cgcgcgtacc agagcacgcg atggaagttc gtgtcgtgaa tgccactcag ccgggattcc
     2461 ggcgggtatg ggacgacgat ccggcgatcg atccgctctg ggtgggcgaa ccagcgctcg
     2521 cggagtccaa cgtcggcgtc gtcatgagcg aagtgccact cacccgtcag gtcgatccgg
     2581 ttctcacgcg caaactgggg attcggcgct gtgctgacag cgtctacgat cgacattgat
     2641 gctctccagg tttccatctc gctcttgacg gacccgcgca ggtttcgcga gccgtcgcca
     2701 ttgttcgtgt tgggggattg tgccactatt
//
LOCUS       DASTMD010000744         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3195885, whole genome shotgun sequence.
ACCESSION   DASTMD010000744 DASTMD010000000
VERSION     DASTMD010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3195885"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1186
                     /locus_tag="VFQ54_11050"
     CDS             101..1186
                     /locus_tag="VFQ54_11050"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEU0165571.1"
                     /translation="MALEELVGTVVRFDLGQVEAFVADTLVAGGVAPDKAQVAASHLL
                     LADLRGVESHGVARLPGYVARLKAGLIDPQAEVTVVHELASTLALDAHNGLGLLAGQD
                     AMGRTIAKAEESGICMTTVRNSNHFGIAGAYAVMAAERGLGGMAMTNASRLVVPTFAS
                     GPMLGTNPIAFAVPVRGARPLIVDMSTSTVAWGKIEIARRAGIPIPAGWAVDADGAPT
                     TDPSAVKGLTPLGGDRTMSGHKGYGLGLMVDILCAVLAGSSWSWDIGRTTELSYSGGI
                     GHFFLAWRIDAFRDPEEFADQMDKVIGEIRAMKVADGAPSNRVLVPGDPEMEAEAENL
                     RLGVPIRTEVLAELAVMANEIGIPPLA"
     gene            1237..1968
                     /locus_tag="VFQ54_11055"
     CDS             1237..1968
                     /locus_tag="VFQ54_11055"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEU0165572.1"
                     /translation="MPTGVDRRPRLADAIIESIAIDILRGDARPGSLLPPEPQLSERY
                     GVSRTVVREAIKRLQSLGLIDVRHGIGTIVLDSTSWDDFDPDMIRIRTNAGFIGDLAD
                     DLFAIRRVIEVQVVADAATNRTQDQLRELEAIVDQMRTPGIDHVVYTDLDIDFHNVLL
                     EAAGNALLVKMMQPVNQIRRIGSLITSAELDYVIPGSIRGHERILQAIAAMDAADARQ
                     QMATHLDEFARDLIRGIESTGAYRR"
BASE COUNT          358 a          645 c          669 g          342 t
ORIGIN      
        1 gtaatcacac gtccgatctt gcgggaacga cccgcgcagc cgaaaatcga gcttcctggc
       61 agtgcgtcgg gcaagaagcg tgtacagcaa ggacccgttc atggcgctcg aggaattggt
      121 cggaaccgtc gtgcgattcg acctcggcca ggtcgaggca ttcgttgcgg atacgctggt
      181 cgcgggcggt gtcgccccgg acaaggcgca ggtcgccgcg tcgcacctgc ttctcgccga
      241 tcttcgcggc gtcgagtcgc acggtgtcgc acgtctcccc ggctatgtcg ctcggctcaa
      301 ggccggactc atcgatccgc aagcagaagt gaccgtcgtt cacgagttgg cttcgaccct
      361 tgcgctcgac gcacacaacg ggctcggtct gctcgcgggg caggacgcga tgggacggac
      421 gatcgcgaag gccgaggagt ccggcatctg catgacgacc gtgcgcaaca gcaaccactt
      481 cgggatcgcc ggcgcctatg ccgtgatggc cgcggaacgc ggactcggtg gcatggcgat
      541 gacgaacgcg tctcgtctcg tcgttccgac cttcgcgagc ggaccgatgt tgggaacgaa
      601 tcccatcgcc ttcgcagtgc cggtgcgcgg cgctcggccg ctcatcgtcg acatgtcgac
      661 gagcaccgtc gcctggggca agatcgagat cgcgcgtcgt gccggcatcc cgatcccggc
      721 tggctgggcg gtcgatgccg acggggcgcc caccaccgat ccctccgccg tcaaggggct
      781 gacgccactc ggcggcgatc gcacgatgtc gggtcacaag ggctacggcc tcgggctgat
      841 ggtcgatatc ctctgcgccg tgctggcggg aagctcctgg agctgggata ttggtcgcac
      901 gacggaactc agctacagcg gcggcatcgg ccacttcttc ctcgcctggc gaatcgacgc
      961 cttccgggat ccggaggagt tcgccgatca gatggacaag gtgatcggcg agattcgtgc
     1021 gatgaaggtc gcggacggag caccaagcaa tcgcgtgctc gttcccggcg atcccgagat
     1081 ggaggctgaa gcagagaatc ttcgcctcgg cgtgccgatc cggacggagg tcctcgccga
     1141 actcgccgtg atggcgaacg agatcgggat cccgccactc gcgtaattca aacggaagcg
     1201 cgcgccactg atgacgcgcg caggatggat caggatatgc ccacaggtgt ggatcgccgg
     1261 ccgcgactgg ccgacgccat catcgaatcg atcgcgatcg acattctgcg cggcgacgcg
     1321 cggccgggat cgttgctccc accggaaccg cagctctccg aacgctacgg cgtgagtcgc
     1381 accgtcgtgc gggaagcgat caagcggctc cagagtctcg gcttgatcga cgtccggcat
     1441 gggatcggca cgatcgtgct cgattcaacg agttgggatg actttgatcc ggacatgatc
     1501 cggatccgga ccaacgccgg tttcatcggg gacctcgccg acgacctctt cgcgattcgt
     1561 cgcgtgatcg aggtgcaggt cgtcgcggac gcggcgacca accggacgca ggaccagctg
     1621 cgcgaactgg aagcgatcgt cgaccagatg cggacaccgg ggatcgatca cgtcgtctac
     1681 accgatctcg acatcgattt tcacaatgtg ctgctcgaag cggccggcaa cgcgctgctc
     1741 gtgaagatga tgcagccggt caaccagatc cgccggatcg gctcgctgat cacatcggcg
     1801 gaactcgact acgtgattcc cggctcgatc cggggtcatg agcggattct ccaggcgatt
     1861 gcggcgatgg acgccgcgga cgcgcgtcag cagatggcga cacatctcga cgagttcgcg
     1921 cgggacctca ttcgtgggat cgaatcgacc ggcgcctacc gccggtagcc gctggcggcg
     1981 tccttcccgc tcacgaacga ccgcgggatc ccag
//
LOCUS       DASTMD010000745         1555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2570098, whole genome shotgun sequence.
ACCESSION   DASTMD010000745 DASTMD010000000
VERSION     DASTMD010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2570098"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VFQ54_11060"
     CDS             <1..811
                     /locus_tag="VFQ54_11060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0165573.1"
                     /translation="VEGDDLRELVPGITPHAVAGAYTANDGHANPILTTHAFATAAVR
                     HGATIALNTRVTAIERTGGRITGLETTNGPIACDWLVLAAGAWSPGLAHELDIDLPIR
                     PMGLQMMATTSMPPLLRQVVGARNRRLSLKQVRSGNYIIGGGWPGDPDLETNLGTPRH
                     ASILGSMDHSSAIFPELRKAQVERVWVGIEAETIDEVPILGPIESIPNLTVATGFTGH
                     GFALSPIVGQLMSELILDGEPSLPLDALSFARFATGDAVSTNPTAPRVHAG"
BASE COUNT          308 a          509 c          468 g          270 t
ORIGIN      
        1 cgtcgaaggc gatgacctgc gcgagcttgt tcccgggatc accccgcacg cggtcgccgg
       61 cgcctacacc gcgaacgacg gccatgcgaa tccgatcctg accacgcacg cctttgccac
      121 cgccgccgtc cgccacggcg cgacgatcgc gctcaacacg cgcgtcaccg cgatcgaacg
      181 cacagggggc cggatcaccg gtctcgaaac gacgaacgga ccgatcgcct gcgattggct
      241 cgtcctcgcg gcgggtgcct ggagccctgg tctggcgcac gaactcgata tcgatcttcc
      301 gatcaggccg atgggactgc agatgatggc gacgacatcg atgcctccgc tgctgcgtca
      361 ggtcgtcggg gcgcgaaacc ggcgcctgag cctgaagcag gttcgaagcg gcaactacat
      421 catcggcggc ggctggccgg gcgatcccga tctcgagacc aacctcggga caccgcgcca
      481 cgcgagcatt ctcggcagca tggaccactc gtcggcgatc ttcccggaac tccggaaggc
      541 acaggtcgag cgagtctggg tgggaatcga ggcggaaacg atcgacgagg tcccgatcct
      601 tggtccgatc gagtcgatcc cgaatctcac cgtggccacg ggttttaccg gacacgggtt
      661 cgccctgagt ccgatcgtcg ggcaactgat gagcgaactg atcctggacg gcgagccgtc
      721 actgccgctg gacgctctct cgttcgcacg cttcgcgacc ggcgatgcgg tgtcgaccaa
      781 tcccaccgcg ccccgcgtcc acgccggcta agcgcacgca ggttcgccac gcggcaacga
      841 tcacgcggaa gcatggagcg cgtatcacca cgatccacgc gtcccgcata accccgtacc
      901 cacgaccctt gtggccgttc ccgtcccggc gacccatgtg gtcgcccccc caacgtggcc
      961 gaaacgcgtc atgcatgacg attgcaaccg ttgccacgag gagcacgatc ctcgggcgaa
     1021 cgggaggcgg gacaggcgtt gccagcgaga gacgaggcgg cgggcgccct cagcataggg
     1081 atcgccgacg ccgacccaag cagggctgtg aacaagtatc cgaacgcctc tcaccggaac
     1141 tggccggttt tgccgaagca ggggtgccgg atcccatcgt aagccacgag caactacccc
     1201 gtggctcctt gttgcttcgc aatcagtcac catacgattg atatcagcaa tacatctttt
     1261 atttatcgtt attcagatat ccgtgatact cattatcata ttcatataaa gtccttggca
     1321 aaacgtgatg gataacctgt tgacaccatg gctcgatgtg cgaaactact cctgtcagca
     1381 taggaagagt tgaagcgctg aacgtttctc tctgcacggg gtatggaccc aggaaaggac
     1441 aatggatgtc tgctgaacga cgcctctccc gaagaggctt tgtcgccacg tctgccgttt
     1501 cgacggcaac cgcgggaatt gcgatggtgg ggggacccgg acgggccgtc ttcgc
//
LOCUS       DASTMD010000746         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2479553, whole genome shotgun sequence.
ACCESSION   DASTMD010000746 DASTMD010000000
VERSION     DASTMD010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2479553"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFQ54_11065"
     CDS             <1..720
                     /locus_tag="VFQ54_11065"
                     /inference="COORDINATES: protein motif:HMM:NF016563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HEU0165574.1"
                     /translation="RHFMDGNSWLGAEDLSDPDFQIGAGCLIDQLVGQYFAHVCGLGY
                     LGDPARIRAALVNDHAYNFRQDLHGHFNHLRTFALGDEAAMLMASYPCGRRPKRPFPY
                     FNEVMTGFEYTAASHMLYEGETALGLEVIEAIRARYDGERRSPFNEAECGHHYARAMA
                     SWAAFLALTGFHYSGVAGTMAFGDLAPGSTTFWSTGDAWGTVAWDGEEAEIAIRHGVA
                     RITSLTIGTSRVPFALAPVVA"
     gene            724..2118
                     /locus_tag="VFQ54_11070"
     CDS             724..2118
                     /locus_tag="VFQ54_11070"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEU0165575.1"
                     /translation="MDVEIEAADEPAVRQRPRELALSAAWNGGLTRKLTTLDGRTVNV
                     VFTGQWTHGFGPDFANAMLEFDGEGLVSGSVEIHYRTSEWASHGHHLDPRYDDVVLHI
                     VSRHDMVETRRSDGRIVPVAVLTVPDEVLFQIDSRLPEIWSRLGGAVCAEELTAEHPG
                     QIIRTLQSLGDERLQERVTRFEGDLSVSPANRVLLGAIFDAFGYSENREPMRELFARL
                     DRHGLLDLIAVGPRTERLLTATTLLLGAGGFLPLAPTDANIARIPASLQSEVERRWLA
                     ASPTWHQDILPPTAWQRGRTRPANHPIVRVTSLAALLANTRTDLLTTTLDLFRHGSDP
                     IDGFRGLARHEHEEMSSLGEGRAIGMIASVLVPITLAYARHVNDPALEEAAIAIWDDL
                     RPADAHRPAKRALAQVAGASKLRGIGERGQQGLLHLDRDYCTPRRCFECPVAADVIRY
                     ERKRPLADTSAAGV"
BASE COUNT          384 a          763 c          699 g          369 t
ORIGIN      
        1 cgccatttca tggacggcaa ttcgtggctg ggcgccgagg atctctccga tcccgacttc
       61 cagatcggcg ccggctgcct gatcgatcag ctcgtcggac agtacttcgc tcacgtctgc
      121 ggtctcggct acctcggcga tccggcgagg attcgggcgg cgctcgtcaa cgatcacgcg
      181 tacaatttcc ggcaggacct tcacggccac ttcaatcacc tccgcacctt cgcgctgggc
      241 gacgaagcag cgatgttgat ggcctcctat ccttgcggcc gccgcccgaa gcgaccgttc
      301 ccctacttca atgaagtcat gaccggtttc gaatataccg ccgcatccca catgctctat
      361 gaaggggaga ctgccctcgg gctggaggtg atcgaggcga ttcgcgcccg ctacgacggc
      421 gagcggcgga gccccttcaa cgaggcggaa tgcggtcacc actacgcacg cgcgatggcg
      481 agctgggcag cctttctcgc gctcacgggc tttcactact ccggcgtcgc gggaacgatg
      541 gcattcggcg acctcgcccc cggctcgacg acattctggt cgaccggaga cgcatggggc
      601 accgtcgcgt gggacggtga ggaggcggag atcgcgattc gccacggcgt ggcgcggatc
      661 acatccctga cgattggcac gtcgcgcgta cctttcgcac tcgcacccgt ggtcgcctag
      721 tcaatggacg tcgagatcga agccgcagat gagccggcag ttcggcagcg accgcgcgaa
      781 ctcgccctaa gcgccgcctg gaacggcggt ctcacgcgca agctgacgac gctcgacggc
      841 cggaccgtca acgtcgtctt cacgggtcag tggacgcacg gattcggacc cgacttcgcc
      901 aacgcgatgc tcgagttcga tggcgaaggg ctcgtctccg gttccgtcga gatccactac
      961 cgcacgagcg aatgggccag ccacggtcac cacctcgacc cacgctacga cgacgtagtc
     1021 ctccacatcg tcagccgcca cgacatggtg gagacacggc gctccgacgg caggatcgtc
     1081 ccggtcgccg tattgaccgt acccgacgaa gtgctcttcc agatcgacag ccggcttccc
     1141 gaaatctggt ccaggctcgg cggagcggtg tgcgcggaag agctgacggc cgaacatccg
     1201 ggtcagatca tccggaccct tcagtcactc ggcgatgagc ggctccagga gcgggtgacc
     1261 cgtttcgagg gcgacctctc cgtgtcaccg gcgaaccgtg tgctccttgg ggccatcttc
     1321 gacgccttcg gctacagtga gaatcgggag ccgatgcgcg agctcttcgc gcggctcgat
     1381 cgacacggtc tcctcgatct gatcgccgtg gggccgcgaa ccgaacgtct cctcaccgca
     1441 acgaccctcc tgctcggcgc cggcggcttc ctcccgctcg cgccgaccga cgccaacatc
     1501 gccaggatcc cggcttccct gcagtcggag gtcgagcgac ggtggctcgc cgcctcgccg
     1561 acgtggcatc aggacatcct gccgccgacg gcgtggcaac gaggccgcac gcgaccagcc
     1621 aatcacccga tcgtgcgcgt gacctcactg gccgcacttc tcgccaacac gcggacagat
     1681 ctcctcacga ccacactcga cctgtttcgc cacggaagcg acccgatcga cggcttccgc
     1741 ggtctggcgc gacacgagca cgaagagatg agttcgctcg gggaaggacg agcgatcggg
     1801 atgatcgcga gcgttctcgt gccgatcacg ctcgcctacg cgcgtcacgt caacgacccg
     1861 gcgctggaag aagccgcgat tgcgatctgg gacgatctgc gtccggccga cgcgcatcgc
     1921 ccggccaagc gcgccctcgc gcaggtggcg ggagcgtcga aactgcgggg gatcggcgag
     1981 cgcggccagc agggattgct gcatctcgat cgcgattact gcacacctcg acgctgcttc
     2041 gagtgtccgg ttgccgccga cgtcatccgc tacgaacgaa aacgcccgct ggcggataca
     2101 tccgcggcgg gcgtgtgatc gagctctgcg ctatctacag cgaaatccga aacggttgca
     2161 gaaggtgagg ctggaccgca tgcgtagtcc gcggtgcgcc cggtcgttcg ggagc
//
LOCUS       DASTMD010000747         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3014207, whole genome shotgun sequence.
ACCESSION   DASTMD010000747 DASTMD010000000
VERSION     DASTMD010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3014207"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFQ54_11075"
     CDS             <1..900
                     /locus_tag="VFQ54_11075"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HEU0165576.1"
                     /translation="NLVVVPASAVATSSSGDVTASVTVDLQSASIPTGITLTYAWQIT
                     RTDGSTARSAIAPVEWADNRYDWTLLTGGVIRLHYFQLDTAFAQQILASAQSTVALLE
                     KPYGVPHSRTIDIWVYPDSTSFRGSLPANTRDTVAGGSFVGFSLIVAVIPNGSTSEVR
                     RVIPHEVSHQMLYQATRNPFTILPVWFDEGMATHIQVGGTATYLGMVINSYRNDALFD
                     LTSLAAAFPYDASQATLAYAASWSAVLYIEETYGTSGIERMIAAFKSGDPADGVIRSA
                     LGISMDQFNRNWKAWIGTLPSTS"
     gene            946..1455
                     /locus_tag="VFQ54_11080"
     CDS             946..1455
                     /locus_tag="VFQ54_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEU0165577.1"
                     /translation="MYWTQKHVLVCTSSHCTQKGAMDVVGRLRLDVVRKGLDTQILVN
                     NCGTIDLCDIGPNMVVYPDNIIYRGVTVKDLPEILEYLKGGPVVERLLVGPNSPEELQ
                     RKAFYARLLDSPSPLSVAEGDALATETGLADGFVAEQLRRGFMAKKPDEEGIEHVSAT
                     KKARARYSL"
     gene            1603..2631
                     /locus_tag="VFQ54_11085"
     CDS             1603..2631
                     /locus_tag="VFQ54_11085"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU0165578.1"
                     /translation="MSSTSQPSLLQRANDGLRRIPFVKRSRRAWAIAGALMVALVVAS
                     VFSNRGFLGWGVIVIAVIMVLPFSQSRALLGAWVPYASMWFIFTFFRSFADETVLAKT
                     LNTKVPHFERWIFGGELPSVRLQDALFNPNHLHWWDYYFTFTHWSYFLIPHVVAVYLW
                     WKHPNIFRQYTYALMLMLTVGLLIYFLIPSNPPWMAPEAINTPGAPTVTRIMNSVGNE
                     LGGAIYKAGYDAIGESNPIAAMPSIHFAVTFMLFWVARTRGRIWTLLALFYAASMGVG
                     LVYLGEHYAIDVTVGGIITTYSWFAVGAWFRRVAPVIGHPLQHDHGSAMTGGEGSTSP
                     IAQPAGAK"
BASE COUNT          490 a          870 c          793 g          536 t
ORIGIN      
        1 aatctcgtcg tcgtgccagc gtccgcggtc gcaacatcga gttcgggaga tgtcactgca
       61 agcgtgaccg tcgatctcca gtcggcgtcg attccaaccg ggatcacgct cacctacgcg
      121 tggcagatca cgaggacaga cggatcgacg gcgcgttccg cgatcgcccc ggtcgaatgg
      181 gccgataacc ggtacgactg gacgctcctg accggcggcg tgattcgtct ccactatttt
      241 caactcgaca cggcgttcgc ccagcagatc ctggcgtcgg cgcagtccac ggtggcgctg
      301 ctcgagaagc cgtacggcgt tccgcacagc cggacgatcg acatctgggt ctaccccgat
      361 tcgacgagct ttcggggctc gctgccggcg aacacccgcg atacggtggc ggggggatcg
      421 ttcgtcggct tctcgctgat cgtcgccgtg atcccgaatg gctcgacgag cgaggtccgg
      481 cgggtcatcc cgcacgaggt cagtcaccag atgctctacc aggccacacg gaacccgttt
      541 acgatcctgc ccgtctggtt cgatgaaggc atggcgacac acatccaggt cggcggcacc
      601 gcgacgtacc tcggcatggt gatcaactcc tatcgaaatg acgcgctctt cgacctcacc
      661 tcacttgcgg ccgcgttccc gtacgacgcg tcccaggcga cgctggcgta cgccgcgagc
      721 tggagcgccg tgctctatat cgaggagacc tatgggacca gcgggatcga gcgtatgatc
      781 gccgcgttca agtcgggcga tccggccgac ggggtgatcc ggtcggcact cggcatctcg
      841 atggaccaat tcaaccgcaa ctggaaagcg tggatcggga cgctcccgtc cacgtcatag
      901 ccgataacca acctctcacc gggatcacag gaaggaagtc catccttgta ctggacgcag
      961 aagcacgttc ttgtctgcac atcgtcccac tgcacgcaga agggcgcgat ggatgtcgtc
     1021 gggcgcctcc ggctcgatgt cgtccgcaag ggcctcgaca cacagatcct cgtcaacaac
     1081 tgcggcacga tcgacctctg cgatatcggc ccgaacatgg tcgtctatcc tgacaacatc
     1141 atctaccgtg gcgtgacggt aaaggatctt cccgaaatcc tcgaatacct caagggtggt
     1201 cctgtcgtcg agcgtctcct ggtgggaccg aactcccccg aggagttgca acggaaggcg
     1261 ttctacgccc ggctgctcga ctccccttcc ccactttccg ttgcagaggg cgatgcactc
     1321 gccactgaga cgggtctcgc cgatgggttc gtggccgaac agttgcgacg gggattcatg
     1381 gcgaagaagc cggacgagga aggaatcgag cacgtcagtg ccacaaagaa ggcacgagcc
     1441 cgttattccc tctaatgccc atttcggggc attcggcaca gatacacgga atcgtagagt
     1501 tcgtataata cttagctcac attcgatgcc gtgtcgcgcg ggagcgaccg gcatctggct
     1561 ggcagtgtcg ccacgacaac cattcgaagg atctgcattg cagtgtcatc aacatcccaa
     1621 ccttccttgc ttcaacgggc caacgacggc ctgaggcgca ttccattcgt caagagatcg
     1681 cgccgcgcct gggcaattgc cggggcgctg atggtcgcgc tcgtcgtcgc ctcggtcttc
     1741 tcgaatcgtg gttttctcgg ctggggcgtg atcgtcatcg ccgtgatcat ggtgctgccg
     1801 ttcagccagt cgcgggcgct gctcggggcc tgggttccct atgcatcgat gtggttcatc
     1861 tttaccttct tccgctcatt cgcggacgag acggtgctgg cgaaaaccct gaacaccaag
     1921 gttccccatt tcgagcgctg gatcttcggt ggcgaactcc catcggttcg cctgcaggat
     1981 gccctgttca acccgaatca cctgcactgg tgggactatt acttcacctt cacccactgg
     2041 tcctattttc tgatcccgca cgtcgtcgcg gtctacctct ggtggaagca tccgaatatc
     2101 ttccggcagt acacctatgc cctgatgctg atgctgaccg tcggcctgct catctacttc
     2161 ctgatcccat cgaacccgcc gtggatggcg ccggaagcga tcaatacgcc gggcgcgcca
     2221 accgtgaccc ggatcatgaa tagcgtcggc aacgagttgg gtggcgcgat ctacaaggcc
     2281 ggctacgatg cgatcggcga atcgaatccc atcgcggcga tgccatcgat ccacttcgcg
     2341 gtgacgttca tgctcttctg ggtcgcgcgg acgcggggcc ggatctggac gctgctcgcg
     2401 ctcttctacg ccgcctcgat gggcgtcggc ctcgtctatc tcggcgagca ctacgcgatc
     2461 gacgtgacgg tcggcggcat catcacgacc tacagctggt tcgccgtcgg cgcctggttt
     2521 cgacgcgtgg cgcccgtcat cggccatccg ctccagcacg atcacggctc agccatgacc
     2581 ggcggcgagg gatcgacatc gccgatcgcc cagccggcgg gcgccaagta gcgatcattc
     2641 cgcgatcctc gccaaccact ttgcgaagac agggttgagc cggtcgtaa
//
LOCUS       DASTMD010000748         1653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_693379, whole genome shotgun sequence.
ACCESSION   DASTMD010000748 DASTMD010000000
VERSION     DASTMD010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693379"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            793..1020
                     /locus_tag="VFQ54_11090"
     CDS             793..1020
                     /locus_tag="VFQ54_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165579.1"
                     /translation="MTAETPNVSLAARAARARENLINALRECGELADAVEHFEGRELL
                     DTLDYIDSLRFVMAESGQLLAGVLRGENLGS"
     gene            complement(1052..>1653)
                     /locus_tag="VFQ54_11095"
     CDS             complement(1052..>1653)
                     /locus_tag="VFQ54_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0165580.1"
                     /translation="TANAVSNDPRVRRTRRMLRDAALELVAERDFSDITISEITRRAD
                     INRATFYQHYIDKDDLIAQALDSLFDEFTASDRSFVATGADFSRDVTPPPLIALFHHL
                     SGRQELYRRLLGNGGSTAFARRHQAFWEEQFLLTRGVLHPEPGSDGPPLELRARAAAA
                     QGHAIIAWWLETGGDESIETVASWTWQFCCDCLGVSGDQ"
BASE COUNT          315 a          531 c          503 g          304 t
ORIGIN      
        1 aaaacatatt tatcgatacc atcaatgcct tggtacgatg cgatcgcact gggatcatcc
       61 tccggtctat ctggtagctg gctcagcggc gaggtcatca ccgcgagttc cccttccggc
      121 aaccggacgc cttcgcgacc aggatcgact ggcccaccga gttcgtcatc gaatacagcc
      181 cggataccgg tggattccag gcgggattca cgaacttcac gcagtatccg gcggcaggga
      241 actagcacat cgcacagtcc acccgggtgt ttcggtcttc gtgggaagga ggggcggcgc
      301 gagcctggtt gagggaagct cgcgccacaa tgggggaggg aagagggctc cgcgagacca
      361 atcttggggg cacggtcatc acggtcttcc ggatgtacct tttgtcatcg ccattcgggc
      421 accacactcg aagatcgtcg atgacgtcag gagtctcggt cggaccggtt ttacgtttcg
      481 gtgtagggct gttccaccga aacccgttcc gcctcgatcc atcaaggcca cctacatcat
      541 gacgcatggc aagtccaccg ccatccgcaa aatcacgcat ttcttatcag gaattcgcgt
      601 tcgcatacgt aatccggatc tacgcagtac gcatgtcgtt gttgggctcg ccccaatgct
      661 gcacgcaccg gaggcctgat ctcgcggagt agtcgccaga tccggttgcc gacgccggac
      721 caacccgcca cacttccccc gacaaggaat cgcatgctcc cgccgaccgg gtttgccagc
      781 agaggatcgc cgatgaccgc cgaaactccg aacgtatcgc ttgccgcccg cgccgcgcgg
      841 gcccgcgaga acctgatcaa cgcactccgc gaatgcggcg aactcgccga tgccgtcgaa
      901 cacttcgagg ggcgggaact cctcgatacc ctcgactaca tcgacagcct gcgcttcgtg
      961 atggcggaat ccggccaact cctcgccggc gtcctccgcg gcgagaatct cggctcgtaa
     1021 cggtcgcttg ccacgcggct catgccgggg atcactggtc gccgctcaca cccaggcagt
     1081 cgcagcagaa ttgccatgtc caggaggcga cggtctcgat cgactcatcc cctccggtct
     1141 cgagccacca ggcgatgatc gcatgcccct gggccgccgc ggcgcgggcc cgaagttcga
     1201 gcggaggccc gtcggatcct ggctcgggat gcagcacacc gcgtgtcagg agaaactgtt
     1261 cctcccagaa tgcctggtgt cgccgggcaa acgccgttga accgccgttc cccaggagtc
     1321 gccggtacag ttcctggcgc ccggaaaggt ggtgaaagag cgcgatcaag ggtggaggcg
     1381 tgacatcccg ggagaagtct gcgcccgtcg cgacgaacga tcggtcactg gccgtgaact
     1441 cgtcgaagag gctgtccagt gcctgggcga tcaggtcgtc cttgtcgatg tagtgctgat
     1501 agaaggtggc gcggttgata tccgcgcggc gggtgatctc cgaaatcgtg atgtccgaaa
     1561 agtctcgctc ggcaacgagc tccaacgcgg catcgcgcag catccgtcgc gtccggcgaa
     1621 cccgtgggtc attcgagacg gcatttgctg tgc
//
LOCUS       DASTMD010000749         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2302207, whole genome shotgun sequence.
ACCESSION   DASTMD010000749 DASTMD010000000
VERSION     DASTMD010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2302207"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFQ54_11100"
     CDS             <1..438
                     /locus_tag="VFQ54_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0165581.1"
                     /translation="LAASEVGIDTFDEAFAKLVDLAAIGPVERHLIHSDLLNFNALVE
                     GDRISAIFDWGCMLYGDFLYDPAWFAFWAPWYPAWRGIDFRAETVKHCAAIGLDVPHF
                     DERFRACAIHIGLSNAAYSAYKGRWEQAGEVCHRALAIAQGTW"
     gene            526..>1678
                     /locus_tag="VFQ54_11105"
     CDS             526..>1678
                     /locus_tag="VFQ54_11105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0165582.1"
                     /translation="MRRNVTIIGAGIVGAALAMELSAIAGIEVTVIEQGAREQLPGST
                     GHAPGYVGILGDAPVLTALADLTTTIYDDLGFGNLPAFTRIGGLEIATSASGVAVLEK
                     RHRMSVEAGLPARLIEPTEAIMLAPDLVGSDGVRAGLHFPADGTARASIATAAMRARA
                     EASGATFVNDATVVGIETADGRITGVRTAASTIGADDVVIASGIWSPRLAATVGFDLP
                     LVPVEHPYVYGPPPEKRISRATTPFIRWPEEHVYARYHGPRFGIGTYDHVPRLVAPED
                     LATGAELLWRGAIFDEAIDRARSLLPASNRFPSDLRLNGVFAITPDNAPLIGRIPAAD
                     GLWTASAIWVTHAAGSAKVLAALMTDRESPVAGISALDPARFAGRDPDEL"
BASE COUNT          269 a          580 c          549 g          280 t
ORIGIN      
        1 ctcgccgcgt ccgaggtcgg catcgacacg ttcgacgagg cgttcgccaa gctggtcgac
       61 ctcgcggcga tcggtcctgt ggagcgacac ctcatccata gcgatctcct gaatttcaat
      121 gcgctcgtcg agggagatcg catcagtgcg atcttcgatt ggggatgcat gctctacggc
      181 gacttcctct acgaccctgc ctggttcgca ttctgggcac cctggtaccc ggcgtggcga
      241 gggatcgact tccgggccga gacggtgaag cactgcgccg cgatcggtct cgacgttccg
      301 catttcgacg agcgtttccg cgcctgcgcg atccatatcg gtctctcgaa cgcggcgtac
      361 tccgcttaca agggacgttg ggagcaggcc ggcgaggtct gtcaccgcgc cctcgcgatc
      421 gcgcaaggaa cctggtaggt agcgtcatca tgctgcctcg aggggtcagt ccggtcagcc
      481 gcgtggagcc atcaaccaac tgatcgcagg ggtgaggagg cgggaatgcg tcgaaacgtc
      541 accattatcg gagccggcat cgtcggcgcg gcactcgcga tggaactctc ggcgatcgcc
      601 gggatcgagg tgacggtaat cgagcaggga gcacgcgaac agctgcccgg ctcgaccggg
      661 cacgcccccg gctacgtcgg gatcctcggt gatgcgcccg tgctgaccgc acttgccgat
      721 ctcacgacaa cgatctacga cgacctcggc ttcggcaacc tccccgcctt cacacgtatc
      781 ggcggactcg agatcgcgac ctcggcgtcc ggcgtcgcgg tcctcgagaa gcgccatcgg
      841 atgtcggtcg aggcaggact ccccgcacgt ctgatcgagc caaccgaggc aatcatgctc
      901 gcgcccgatc tcgtcggatc cgacggcgtt cgcgccggcc tccacttccc agctgacggg
      961 acggcccgcg ccagcatcgc caccgccgcc atgcgggctc gcgccgaggc gagcggcgcg
     1021 accttcgtca acgatgccac ggtcgtgggc atcgagacag ctgatggacg aattaccggc
     1081 gtgcggaccg ccgcgtcgac gattggagcg gacgatgtcg tgatcgcgag cgggatctgg
     1141 agtccccgcc tggcggcgac ggtcggcttc gatttgccgc tggtccccgt cgaacatccg
     1201 tatgtctatg ggccgccgcc ggagaagcgg atctcccgcg cgacgacacc gttcatccgc
     1261 tggccggagg aacacgtcta cgctcgctat cacggcccac ggtttggaat cggcacctac
     1321 gatcacgtcc cgcgattggt cgcgcccgag gacctcgcga ccggggccga actcctgtgg
     1381 cgcggcgcga tcttcgacga ggcgatcgac cgggcacgat cgcttctccc ggcctcgaac
     1441 cgctttccat cggatctcag gctgaacggc gtcttcgcga tcaccccgga caatgcgccc
     1501 ctgatcgggc ggatcccggc cgccgacggc ctctggaccg cgagcgcgat ctgggtgacg
     1561 cacgccgcgg gcagcgcgaa ggtcctcgcc gcgttgatga cggaccggga gtcacccgtt
     1621 gccgggatca gcgcactcga cccggcgcgg ttcgccggcc gggaccccga cgagctcc
//
LOCUS       DASTMD010000750         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1319906, whole genome shotgun sequence.
ACCESSION   DASTMD010000750 DASTMD010000000
VERSION     DASTMD010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319906"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VFQ54_11110"
     CDS             <1..927
                     /locus_tag="VFQ54_11110"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0165583.1"
                     /translation="PVDRLLPELADRQVLRSLESEATDTVPANRAITLRDLLTFTFGQ
                     GLVFAWPGTYPIQTAIEEAGLGPHPPQPASDPEPDEWMRRLGTLPLIYQPGERWLYNT
                     GSDVLSVLIARASGQDLETFMGERIFDPLGMKDTGFSVPAENIDRLTTNYGTNFETGA
                     LELYDAAEGGAWSTAPAFPSGAGGLVSTIDDYLAFAQMLLNGGKLGNTRILSRPTVEL
                     MTTNQLTPEQRVSAGFLDQGWGFGVAMVLERRDVIPVGAYGWDGGLGTTWRNDPAEDL
                     ISIMLTEASWTSSTPPAICRDFWTTAYASLDD"
     gene            complement(1030..1491)
                     /locus_tag="VFQ54_11115"
     CDS             complement(1030..1491)
                     /locus_tag="VFQ54_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165584.1"
                     /translation="MYYWTDLYLHHDASNEQISRAIGDVFRVSPARIAIHPVMSEETD
                     VAWARPDLYALVQRDDFLETEERDPIFPIKLMVIVRQDNPEIDDHEALVSITRALRQP
                     VLSDNGDDGEDVWRLYWPDGTSTPVALDDDGRFDLKGTDLVRLESSQHVVA"
     gene            complement(1499..2047)
                     /locus_tag="VFQ54_11120"
     CDS             complement(1499..2047)
                     /locus_tag="VFQ54_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165585.1"
                     /translation="MTRIVPQAVKIATYIASGDGRLRVGSRILFARHLGRPALEPGDE
                     GSSAPFREFTRIDERHYVTRIKKNSISRRDGKNTMILPGFEDAIEIDRRLINAGKARR
                     IGNVWHINGRMYESHGETGAFYPTGGEGTLDIDRGTHRALVILKGYNGPTGAAHFRLR
                     KERGIAEEQIEQALWLWNLRPE"
     gene            2087..3130
                     /locus_tag="VFQ54_11125"
     CDS             2087..3130
                     /locus_tag="VFQ54_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0165586.1"
                     /translation="MKITHVDVEFASIAKSRPIRDALQVLDQNGHCRVTIHADEGFSG
                     TSETYFGRVASSPAILAHILNDELIPAIVGEDPFLIRVIRDKLWALTDYHGSTGYALF
                     GIAALDQALWDLVGKATGQPVWRLLGASRTTVPSYAMVGWLELDIDGLKSASTKAMEQ
                     GFRGVKMKVGAETLTEDVQRIEAVRDAIGPDAKLMVDANQVFTVIEATRRGLIFQEMG
                     CTWFEEPIRADDTEGHVQLAQRLQIPIATGENRYGISQFRDLFTRGGVGIVQPDLRRA
                     GGLTECFEIGLMAHGFNIPYASHGGGAHLHLLAALPNTIYMESSLLPENGSLKLVDGC
                     YPLPEEPGLSGWH"
BASE COUNT          603 a         1067 c         1016 g          614 t
ORIGIN      
        1 ccggtcgacc gcctcctgcc ggaactcgcg gatcggcagg tgctccgatc gctcgaatcg
       61 gaggcgaccg atacggtacc ggcgaaccgc gcgatcacgc tgcgggatct gctcaccttc
      121 accttcggtc agggcctggt cttcgcctgg cccgggacct acccgatcca gaccgcgatt
      181 gaggaggccg ggctggggcc gcatccgccc cagccagcca gcgaccctga gccagacgaa
      241 tggatgcgcc gtctgggtac gctgccactc atctaccagc caggcgagcg gtggctctac
      301 aacaccggat cggacgtgct cagtgtcctg atcgcgcgcg catcgggcca ggatctcgag
      361 accttcatgg gcgagcgcat cttcgatccg ctcgggatga aggacaccgg cttcagcgtg
      421 ccggctgaga atatcgatcg cctgaccacg aattacggga cgaacttcga gaccggtgcg
      481 ctcgaactct acgacgccgc cgaaggcggg gcctggagca cggctcccgc cttcccgagc
      541 ggggctggtg gcctggtttc aacgattgat gattatctcg ccttcgccca gatgctgctc
      601 aacggcggca agctcggcaa cacgcgtatc ctctcgcgtc cgaccgtgga actgatgacg
      661 accaaccagc tgaccccgga gcagcgggtg tctgccggat tcctcgatca gggatggggt
      721 ttcggggtcg cgatggtgct ggagcgccgc gatgtgatcc cggtcggcgc ctatggctgg
      781 gacggcgggc ttggcaccac ctggcgcaac gacccggcgg aggatctgat ctcgatcatg
      841 ctgacggaag caagctggac atcctcgact ccgccggcga tctgccgtga tttctggacc
      901 actgcctacg cctcgctcga cgactgatgc gggcattcag caccgcgaga accgagaggc
      961 gatttcatgc gaacgcgccg gctggtgccc caacaccacg ccggcgcgtt ccatctccga
     1021 catctggtgt caagccacga cgtgttggga ggattcgagg cggacgagat cagtaccctt
     1081 gagatcgaaa cgtccgtcat cgtcaagcgc gactggtgtc gaggtaccgt ctggccaata
     1141 gagacgccag acgtcctcac catcgtcccc gttatcgctc aggactggct gcctcaacgc
     1201 gcgcgtgatc gagacaaggg cctcatgatc atcaatctcc gggttgtcct gacgaacgat
     1261 gaccatcagt ttgattggaa atatcggatc tcgttcctcc gtctcgagaa agtcgtcccg
     1321 ctgcacgagc gcatagagat cggggcgtgc ccaggccacg tcggtttcct cggacatcac
     1381 aggatgaatc gcgatacgcg caggggagac gcggaatacg tcgccgatcg cacgcgagat
     1441 ctgctcgttc gaggcatcgt gatgaagata gaggtcggtc cagtagtaca tatctgcctc
     1501 attccggacg gagattccag agccacaggg cctgttcaat ctgttcctcc gcgataccgc
     1561 gttcctttct caggcgaaag tgtgcggctc ctgttggacc attgtatccc ttcagaataa
     1621 ccagggcgcg atgggttccc cgatcgatat cgagggtgcc ttcccctccc gttgggtaga
     1681 atgctcccgt ttcaccatgc gactcataca tgcgtccgtt gatatgccac acattgccga
     1741 ttcgccgcgc cttccccgca ttgatgagac gacgatcaat ttcgatggcg tcctcgaagc
     1801 cgggaaggat catcgtgttc ttcccgtctc tccgcgagat cgagtttttc ttgatccgcg
     1861 tcacgtagtg gcgctcgtcg atcctcgtga actcccgaaa cggagcggaa gagccctcgt
     1921 cacctggttc cagagccggc ctccccaaat gtcgcgcgaa cagtattctg gatcccactc
     1981 gtagcctacc atcgccactg gcaatgtatg tcgcgatctt gacggcctgc ggtacgatgc
     2041 gggtcattcc atatccgcca tccacccgca caaaggcagg cactccgtga agatcaccca
     2101 cgtcgatgtc gagttcgcct cgatcgcaaa gagccgtccg attcgcgacg cgctccaggt
     2161 cctcgaccag aacgggcact gccgtgtcac gatccatgcg gacgagggat tctccgggac
     2221 gagcgagacc tacttcggcc gcgtcgcgtc atcgcccgcg atcctcgccc acatcctgaa
     2281 cgacgaactg atcccggcaa tcgtcggtga ggatcccttc ctgatccgcg tcatccgcga
     2341 caagctctgg gcgctcaccg actaccacgg ctcgacggga tacgcgctct tcgggatcgc
     2401 cgcgctcgac caggcgctct gggatctcgt cggcaaggcg accgggcagc cggtctggcg
     2461 cctgctcggc gcgtcgcgca cgacggtgcc ttcctacgcg atggtcggct ggctcgagct
     2521 cgacatcgat gggctgaaat cagccagcac gaaggcgatg gagcagggct ttcgcggcgt
     2581 gaagatgaag gtcggggccg agacgttgac cgaggatgtg cagcggatcg aagccgtgcg
     2641 cgacgcgatc ggtccggatg cgaagctgat ggtcgatgcg aaccaggtct tcacggtgat
     2701 cgaggcaaca cgacgtggtc tcatcttcca ggagatgggc tgcacctggt tcgaggagcc
     2761 gattcgcgct gacgacaccg aggggcacgt ccagctcgcg cagcggctgc agatcccgat
     2821 cgcgactggt gagaatcgct acggcatttc ccagttccgc gatctcttca cgcgcggtgg
     2881 ggtcggaatc gtccagcccg acctccgccg cgccggtggc ctgacggagt gcttcgagat
     2941 cggcctgatg gcgcacgggt tcaacatccc ctacgcctcg cacgggggcg gggcgcacct
     3001 gcacctgctg gcggcgctcc cgaacacgat ctatatggaa agcagcctgc tgccggagaa
     3061 cggttcgctg aagctcgtcg acggctgcta cccgctcccc gaggagcccg gcctcagcgg
     3121 ctggcactga cgcgtcgcga cgagagcctt gtctgagatc agcaaagggg gcgacataaa
     3181 agtcgccccc tactcacgac cggtatccgt ccggcgatcg ttcgtggaca gccccgtgcc
     3241 ctgccgaggc aggcgagggc tacgcgggaa cctgggtctg ttgacctcga ccggctactg
//
LOCUS       DASTMD010000751         1654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_782480, whole genome shotgun sequence.
ACCESSION   DASTMD010000751 DASTMD010000000
VERSION     DASTMD010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782480"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VFQ54_11130"
     CDS             complement(<1..1300)
                     /locus_tag="VFQ54_11130"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165587.1"
                     /translation="MSSVIDEIVGDESVLLTEEITPEAELAEEPAPRRGAFSALEVPA
                     FARYWTTLCLSMTGTWVRITAMGVLVYDITGDPFKLGLISFVQAAPELIVGPIAGAYL
                     DRVDRRKVLIGLNLIFISMMVLVATLVKTGAVQYWHLIIVALVIGTATGFDWPARLAL
                     APTLVDRSRLQSAVAINSAAFNGARVLGPTIGGWLIGVAGLAFCFGFTAIAALPFTLI
                     LLTLTAIRRPTSIHATDPGASPFTTLIEGYRYIWRTPKIRGLLSVDIVPIALGMSYAT
                     MAPAIAKDVLHLPDGGLGWLLAANGVGSLTGTIAVAFLSGVQHRGRIVIGGIAAFGVM
                     LVVFGLSGSLWISLPAMLLLGLVFATYGTMNDTLVQTNIDETYRGRIVAVQTMLWGLT
                     PIGGLLAGFLAGVFNVQWAVAINGMLVLCYVPYLWFRTPVR"
     gene            complement(1293..>1654)
                     /locus_tag="VFQ54_11135"
     CDS             complement(1293..>1654)
                     /locus_tag="VFQ54_11135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0165588.1"
                     /translation="ITSWQDGIDGDHKGYDGLILGMPDGSCHLEFTHHRDGSPAPIPS
                     RDSLLVFYLLARDQLDQVVARFHAYGHREVEPENPWWANDGHTFEDPDGWRVVLMLGH
                     GVGATPQAFEDRDQRHV"
BASE COUNT          313 a          528 c          565 g          248 t
ORIGIN      
        1 gacgcaccgg tgttcggaac cagagatagg gcacgtagca gagcacgagc atgccgttga
       61 tcgccaccgc ccactgcacg ttgaagacgc cggcgagaaa accggcgagg agaccgccga
      121 tcggcgtcag tccccagagc atcgtctgca ccgcgacgat gcggccccgg taggtttcgt
      181 cgatattcgt ctgcaccagc gtgtcgttca tcgtgccgta ggtggcgaaa acgagaccga
      241 gcagcagcat cgcggggagt gagatccaga gacttcccga gaggccgaag acgaccagca
      301 tcaccccgaa cgcggcgatc ccgccgatca cgatccggcc ccgatgctgg acgccggaga
      361 ggaaggcgac cgcgatcgtt cccgtcagcg agccgacgcc gttggcggca agcaaccagc
      421 caaggccgcc atccggcagg tggagcacgt ccttcgcgat cgccggcgcc atcgtcgcgt
      481 agctcatccc gagcgcgatc ggcacgatat cgaccgagag caggccgcga atcttcggtg
      541 tccgccagat gtacctgtag ccctcgatca gggtcgtgaa agggctggcg ccggggtcgg
      601 tggcatgaat cgaggttggc cgccggatcg cggtgagcgt cagcaggatc agggtgaatg
      661 gcaacgcggc gatcgccgtg aagccgaagc agaatgccag ccccgccacg ccgatcagcc
      721 agccgccgat cgtcggcccg aggacgcggg cgccgttgaa ggccgccgag ttgatcgcga
      781 ccgcactctg gagccgcgac cggtcgacga gtgtcggggc gagcgcgagt cgggccggcc
      841 agtcgaatcc ggtcgccgtg ccgatcacga gggcgacgat gatgaggtgc cagtactgga
      901 ccgcgccggt tttcacgagg gtcgcgacga gcaccatcat cgagatgaag atcaggttga
      961 ggccgatcag gaccttccgg cgatcgacgc ggtcgagata ggcgccggcg atcggcccga
     1021 cgatcagctc tggtgccgcc tgcacgaagc tgatcaaacc gagcttgaac gggtcgcccg
     1081 tgatgtcgta gacgaggaca cccatcgccg tgatccggac ccaggttccg gtcatcgaga
     1141 ggcagagtgt cgtccagtag cgggcgaagg cgggaacctc gagcgcgctg aaggcgccgc
     1201 gccggggagc gggttcctcc gcgagttcgg cttccggcgt aatttcctcg gtcaggagga
     1261 cggattcgtc gccgacgatc tcgtcgatca cgctagacat gacgctgatc tcgatcctcg
     1321 aatgcctggg gcgtcgcgcc aacgccatgt ccgagcatga gcacgacacg ccagccgtcg
     1381 ggatcctcga aggtgtggcc gtcattggcc caccacggat tctccggctc gacctcgcgg
     1441 tgcccgtagg catggaagcg cgccacgacc tggtcgagct gatcccgcgc gagcaggtag
     1501 aagaccaaca ggctatcccg actcgggatc ggggcggggc tgccgtcgcg atgatgcgtg
     1561 aattcgagat ggcagctgcc gtccggcatg ccgaggatca gcccgtcgta gcccttgtga
     1621 tccccatcga tcccgtcctg ccacgaggtg atcc
//
LOCUS       DASTMD010000752         3529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2927633, whole genome shotgun sequence.
ACCESSION   DASTMD010000752 DASTMD010000000
VERSION     DASTMD010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2927633"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(477..1547)
                     /locus_tag="VFQ54_11140"
                     /pseudo
     CDS             complement(477..1547)
                     /locus_tag="VFQ54_11140"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     regulatory      complement(1548..1660)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1768..2217)
                     /locus_tag="VFQ54_11145"
     CDS             complement(1768..2217)
                     /locus_tag="VFQ54_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165589.1"
                     /translation="MAHENWTESPGVAPVTTALIPVSFGKLNCAALLPLIVIAGTAAA
                     AEKEFAFARAGDVEVLPRVTVAASVGETVPAAPVVAGCVPSGSVLTGSFITTGLASCA
                     HTVSSGDTAMPKIIMSPRTHRNRRITPSQAHDYIIDFVRTGPPRNHA"
     gene            2249..>3529
                     /locus_tag="VFQ54_11150"
     CDS             2249..>3529
                     /locus_tag="VFQ54_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165590.1"
                     /translation="MGSTLYPVDESGSVSYNWVGFADITYWIQAYDYNSATNASTYSN
                     CMRIDWGSSSVPTDTPVPPTATSTAGPSPTPTNTVPPTATSSPTNTPTLAPTATATAT
                     PVPEIAVNGSTNAITSVQPNDTVTFFYSNVQYAWLYADGTCSYYAGTSFPTPDDGGST
                     VSHTAADWQSLASGSYAFSFRGFDANGQPNTPCLAVSVPFDSTGAPVLDIDGSTAATI
                     QRANGTGVTANISGATPNSTTTLYTSFTSCPYQSPNTTAVRDLPVDGGGLASASLFGE
                     SDTTEYVQAKAESGPWTTCIQITWSAPSTATTAATETPVPTETSTPTSTTVPTQTPTP
                     TITTTPTETLTPSVTPTPSPTPVPSLTINGGTDTLIQLNLGDFFYYQAENVPMLLFFS
                     GGGCLPGNFLNESPNPNSGSFSTLGFGPYYFINNQ"
BASE COUNT          669 a         1145 c         1082 g          633 t
ORIGIN      
        1 ctcgaggaat acatgtcgct acgggagttg gtcgatctgc tcgaccagcc gaagaacgcc
       61 cagcgcctga ttcggtcaat cgcggacctc gaggctggtc gtggcacagg tcgggatctt
      121 ctcgatgcag atgccccagc acaacgacgt cgagatccgc gggatctgta gcgcgggatt
      181 tcatatcgcg cggcggccac gagtgtcgtg gcgacaattc ctcggacgac catgagggag
      241 aggcattgtc catggggaca atcaggccgg gcgttgcccg gcacgcgctt cgcgcgggga
      301 tttctcggca agctcgaaat gacagttgtg cggacattgg gagtccggaa gcatggcgac
      361 gctggcgatc atgtcagcct gacggatgga catagaagtc cattcctacg cacgaccgct
      421 gtatcaacga cccatatgca aggatggtcc gctggcaacc atgaggctga cctgacttac
      481 gaagcgggtg atgcctcggg cgacgctccg ggggtggcga ggatcgaggc aatcggcagc
      541 agatccgtac ggtaggctgc ggcgatatcc accttctcgt cgaggatctt ctgccccttc
      601 atccagtcgc cgtaggcctg ccaacgggca tcggtctgcg tgccgaatgg aagcttcccg
      661 ctgtccgtcc acaagggaat gagcaggtcg attcccttcc gctcgaccgt cttgtcgaca
      721 tcggtgcttg ccttggcaag gatgtcgatc gccgcgtcgg gatcggtcgc ggcggcatcg
      781 tagccgcgct ggatcgcacc gagcagccct gtcaccgttt ccccgttgtc cttcgcgtag
      841 tcttcgcccg tcacgatcat caactagtag tagtcaggga caccccactg ctcgacgcgg
      901 aggtagccaa ccggcgttcc ttcctgctcg gcgaggatcg actcgtgggt ccagtaggca
      961 ccgatcacgg aatcggcctt gccggagagc agcgccggca gcaggtcgta gccgacattg
     1021 atgaccttca cgtcgtcgat cgtcaggccg tccgccttga ggatcgtgcc gagaatcgcg
     1081 tcgtcgctcg gcacgccggc gatgccgacg ctctttccct tgagatcggc cgggcgcgtg
     1141 atcccggcat ccttcttgaa catcaggcag ttcagcggat gctggaccag ggcgccgacg
     1201 gcgatcgtcg ggacgtcctg cgcgcgcgcg aggagaatat ccgtctggta ggagataccg
     1261 aaggtgtcgc gaccggcgcc gacggtctgc aggaccgtcg tggggtcggc cggcgtgtag
     1321 accttgacgt cgagcccggc atcggagaag tagccgttcg cctgggcaac gaagatgccc
     1381 gcgtgctggg cggtcggata ccagtcgagt gcgagcgaga ccttggtcgg cgactgcgcg
     1441 gcggcaggac gcgcgattcg cgagccggcc gcggcgccaa gagccagtgc ggcacctgcc
     1501 cggacgaagg cgcgtcgcga cgaacgagat gatccgatgg tcttcatggg aatgatcctc
     1561 cgaattcctg cgcgggcatt gccccgatca ggttcaacgg tcggcgacga accggtcgcc
     1621 ctctcagccc ggcttcgccc gagctcccgt tccgagtggt tgtggggaga gtatagatga
     1681 agggtcggtg atagcgctgg atcgttcgaa ttccgttcct cccggcgacc aaatacgttg
     1741 ctccaaacgt ggcagattcc ccgaccatca tgcatgattt cgtgggggtc ccgtacgaac
     1801 gaaatcaatg atgtaatcgt gtgcctggga gggtgtgatg cgccgattcc ggtgggttct
     1861 ggggctcatg atgatcttgg gcatggcggt atcgccgctg cttaccgtgt gggcgcagga
     1921 cgccagcccg gtggtgatga acgaccctgt gagcacggaa ccggacggga cgcaacccgc
     1981 cacgacgggg gccgcaggga cagtctcgcc aacagaagcg gcaaccgtca cccttggcag
     2041 cacctccacg tcgcctgccc gtgcgaacgc aaactccttc tctgccgccg ccgcagtgcc
     2101 ggcgatgacg atcaacggca gcaatgctgc gcaattgagc ttgccgaatg acaccgggat
     2161 caacgcggtg gtgacgggag ctacaccggg cgactctgtc cagttttcgt gggccatggg
     2221 cggctgcccg gatacatggg gccaattcat gggctccacg ttgtacccgg tggatgagag
     2281 tggatcggtt tcctataact gggttggctt cgcggatatc acatactgga ttcaggcgta
     2341 cgactataat tcggccacaa atgcaagcac ctactccaac tgtatgcgca tcgattgggg
     2401 cagcagctcg gtcccgaccg atacgcctgt cccgccgacg gcgaccagta ccgctggccc
     2461 ctcaccaacg ccgacgaaca ccgtcccccc gactgcaacg agtagcccca caaatacgcc
     2521 cacgctcgcc ccgaccgcca cggcaacggc aacaccagtc ccggagattg cggtcaacgg
     2581 ctcgacgaat gccataacct cggtccagcc aaacgatacg gtgacgttct tctattcaaa
     2641 cgtgcagtat gcgtggctct acgcagatgg aacctgctcc tactacgcgg gaacgtcctt
     2701 cccgacaccg gatgacggcg gatcgaccgt ctcgcacacc gccgccgatt ggcagtcgct
     2761 cgcctccggg tcgtacgcct tcagcttcag gggcttcgac gccaacggtc aacccaacac
     2821 cccctgcctc gcggtctcgg tccccttcga ctcgacgggt gcgccggtgc tcgacatcga
     2881 tggcagcacg gccgcgacca tccagcgcgc taacggcacg ggagtgacgg cgaatatcag
     2941 cggtgcgacg ccaaactcga cgactacgct ctacacctcg ttcacctcgt gtccctacca
     3001 gtcgccaaat accacggccg tgcgtgacct cccggtggac gggggtggac tggcgagcgc
     3061 ctcgctcttt ggggaatctg acacgaccga gtatgtgcag gccaaggcag agagcggtcc
     3121 gtggacgacc tgcatccaga tcacctggag cgcgccatcg acggccacga ccgcggccac
     3181 cgagacgccg gtgccgaccg aaacgtcgac tccaacatcc accacagtgc cgacccaaac
     3241 cccaacgccc acgatcacca ccacgccgac cgaaaccctc accccatcgg tcacgccgac
     3301 gccatccccg acgccggtgc cgagtctgac gatcaacggt gggacggaca cgctgattca
     3361 gttgaacctc ggcgatttct tctattatca ggccgagaat gtcccgatgc tcctcttctt
     3421 cagcggcggc ggctgccttc ccgggaactt tctcaacgaa tccccgaacc ccaacagcgg
     3481 ctccttcagc acgcttggct tcgggccgta ctatttcatc aacaaccag
//
LOCUS       DASTMD010000753         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_517698, whole genome shotgun sequence.
ACCESSION   DASTMD010000753 DASTMD010000000
VERSION     DASTMD010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517698"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VFQ54_11155"
     CDS             complement(<1..439)
                     /locus_tag="VFQ54_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU0165591.1"
                     /translation="MLAEAGGRPLLAWSLDAIDGAATVRDVVIVAGAHTEPAIADLVA
                     GGGWSKRARIVRGGTSRHESVVAGVNAIADDVDVVLIHDAARPLVSPEQFDACADLAR
                     ETGAAILAAPVADTLKRVKGGTIETTVSRADLWGAQTPQGFRLA"
     gene            complement(355..1365)
                     /locus_tag="VFQ54_11160"
     CDS             complement(355..1365)
                     /locus_tag="VFQ54_11160"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU0165592.1"
                     /translation="MEPVSRIDLHAHTTASDGMLSPVALVDLARERGLSVLGITDHDT
                     IDGLAEATVAAASSGPALVPGVELSTSIGGHDVHILGYFIDAGDERLVAKLHARADAR
                     KRRVVRMVEQLRALGYPIEIEPILAQADAGTIGRPHVARALVDLGVVSSVGEAFDRFL
                     QPGKPGWAPYERFIPEEAIGLIVENRAVPVLAHPYTTGDVEATLGRLVPAGLRGLEVY
                     YGEYSPAQHAGLLDVARRWGLLPTGGSDFHGPGFKEGRDLGTAPVPEEVFTDLAALYD
                     ELTARRVTPRMQRSPRSLSSPRGKASGSARVARCSLKLAGGRFSPGVSMRSTARRPFA
                     TS"
     gene            complement(1402..1689)
                     /locus_tag="VFQ54_11165"
     CDS             complement(1402..1689)
                     /locus_tag="VFQ54_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HEU0165593.1"
                     /translation="MDPEYAGVVGEVLKVSARSRPSAVAGAIAGVVRESGRAEVQAIG
                     AGATNQAVKAVAIARDYLRETGIDAVCLPAFIDVTIENEDRTAIRLVVEAR"
     gene            complement(2376..>3285)
                     /gene="rny"
                     /locus_tag="VFQ54_11170"
     CDS             complement(2376..>3285)
                     /gene="rny"
                     /locus_tag="VFQ54_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU0165594.1"
                     /translation="DDMKGRIIGREGRNIRALEQATGVDLIVDDTPEAVTISGFDPVR
                     REVARRSLLKLLQDGRIHPARIEEVVTKTQFELEQIMREEGERIAYEANVPGLHPDLI
                     KLLGRLKFRTSYGQNVLGHSLEVSILCANLAAELGADINIAKTAGLLHDIGKAVDHEV
                     EGPHALIGADIAKRLGRSSKIVHAIAAHHNEEEPQTIEAFIVQTADAISGARPGARRE
                     MVETYIKRLEALEGVANSFEGVDKSFAIQAGREVRILVKPNSIDDLGATRLAQDVVKK
                     IEETLEYPGQIKVTVIRETRAVEYAR"
BASE COUNT          557 a         1120 c          999 g          609 t
ORIGIN      
        1 cggcgaggcg aaacccctgt ggcgtctgcg cgccccagag atcggcccgg gagacggttg
       61 tctcgattgt cccacccttg acccgcttga gggtatcggc gaccggcgcc gccaggatcg
      121 cggcgccggt ctcgcgcgcg aggtcggcac acgcgtcgaa ctgctcggga ctcacgagtg
      181 gccgcgccgc gtcgtggatc aggacgacat cgacgtcatc cgcaatcgcg ttcaccccgg
      241 caacgaccga ctcatgacga ctcgtgcccc cccgcacgat ccgtgcacgc ttcgaccacc
      301 cgcctcccgc cacgagatcg gcgatcgccg gctcggtatg tgcccccgcc acgatcacga
      361 cgtcgcgaac ggtcgccgcg ccgtcgatcg catcgagact ccaggcgaga agcggccgcc
      421 cgccagcttc agcgagcacc ttgccactct cgccgaaccg ctcgcctttc cccgcggcga
      481 cgacaacgac cgtggcgaac gctgcatcct cggcgtgact cgtcgcgcgg tcaattcgtc
      541 gtagagcgcg gcgagatcgg tgaacacctc ttcgggaacg ggagcggtgc cgagatcgcg
      601 tccttccttg aagcctgggc cgtggaagtc actgccaccg gtcggcagca atccccatcg
      661 ccgcgccaca tcgaggagcc cggcgtgctg ggccggagag tactcgccgt aatacacttc
      721 gagtccacgc aatccggcag gaacgagtcg tccaagggtc gcttcgacat cgccagtggt
      781 atacgggtgt gcgagaacag gaaccgcgcg attctcgacg atcagaccga tcgcctcttc
      841 ggggatgaag cgctcatacg gggcccaacc gggctttcct ggctgcagga agcgatcgaa
      901 cgcctcgcca accgaactca cgacaccgag gtccaccagc gcccgcgcaa cgtggggccg
      961 accgatcgtg ccggcatccg cctgcgcgag aatcggctcg atctcgatcg gatagccaag
     1021 ggcgcgaagc tgctcgacca tgcgaacgac gcgacgcttt cgcgcgtccg ccctggcgtg
     1081 gagtttggcg accagccgct catcccctgc atcgatgaag tagcccagga tatggacatc
     1141 gtggccgccg atactcgtcg agagctcgac gccgggcacc agtgcgggtc cgctgctcgc
     1201 tgcggcgacc gtcgcctcgg caaggccgtc gatcgtgtca tgatcggtga tgccgagcac
     1261 ggaaagcccc cgttccctgg ccagatcgac cagggcaacg ggggaaagca ttccgtcgga
     1321 tgcggttgtg tgggcgtgga gatcgatccg cgaaaccggt tccattgcga tgttctccgg
     1381 ttccgatccc gatctcgcgc cctaccgggc ttcgacgacg aggcggatcg ccgtccggtc
     1441 ctcgttctcg atcgtgacgt cgatgaaggc aggcaggcaa acggcgtcga tccccgtctc
     1501 ccgaaggtag tcacgcgcaa tggcgacggc cttcacggcc tggttcgtcg ccccggcgcc
     1561 gatcgcctgg acttcggcgc gtcctgattc ccggacaaca cccgcgatcg caccggcgac
     1621 ggcgcttggc cgtgaccgtg cggagacctt cagcacctca ccgacgacac cggcatactc
     1681 gggatcgatc accagggcgg gcgcggcggt cacgccaatc agcgcgtcct cctcgagatg
     1741 tccgttcccg ttggcgaagg gactcgccac gaacgcgggc tcctcgatct cgtccgccgg
     1801 ggcgtcccag gtcgtcgtcg tgaccgagcg atactcgatc tcgacggttt cctcatgagc
     1861 gatctccatt ggacctgcct cggcgacggc ttccagctct tcctcgacct cgggctcctg
     1921 ctcgtcagcg tcgggcggga aacgtgatcc ctccgggctg atcttgatgt cccgaagatc
     1981 gagtttgctg aagaaacgat tcatgcgtga ggtcccccta attcgttccc cactaacccg
     2041 aagaccgggt gcaatgatgg ttgccgggtg atgactccgg ttatgggatc gtcacgatcc
     2101 cgatccggaa gcccggcaag aacgccccac gatccgtctc tcccacggat cgccccgggt
     2161 tgccagccca agcgtcgccg ttaagtctac gctgtccatc ccatttggcg cacggttccg
     2221 ggaaaactgg catgaagggt ccaccgttct tcctgtgatg gtcacccgaa aacacaaaac
     2281 gggcgcatcg gctgttcgcc gatgcgcctg tcctgaacgg gacatgtcct gagctgaaga
     2341 acgtcgcgac gctttccggc ccagcccggg tcggattacc gtgcgtattc gaccgcgcgc
     2401 gtttcgcgga tgacggtgac cttgatctga cccggatatt ccagggtctc ctcgatcttc
     2461 ttcaccacat cctgcgccag ccgtgtcgct cccagatcat cgatcgagtt cggcttcacc
     2521 aggatccgga cctcacgtcc ggcctggatc gcgaacgact tgtccacgcc ttcgaaggaa
     2581 ttcgcaaccc cttcaagtgc ttcaaggcgc ttgatgtagg tctcgaccat ctcccgccgt
     2641 gcgcctggcc gtgcgccgct gatggcgtct gccgtctgca cgatgaaggc ttcgatggtc
     2701 tgcggctctt cctcgttgtg gtgcgccgcg attgcatgga cgatcttcga agaccgcccc
     2761 aggcgcttgg cgatgtccgc gccaatcagc gcgtgtggcc cttcgacctc atggtcgacg
     2821 gccttgccga tatcgtgcag aagaccagcc gtcttcgcga tattgatatc ggcacccagc
     2881 tccgcggcaa gattggcgca gaggatcgag acctcgagcg agtgacccaa cacgttctgc
     2941 ccgtaactcg tgcggaactt gagtcgcccg aggagcttga tcagatccgg atgcagaccg
     3001 gggacgttcg cctcataggc gatccgttct ccctcttccc gcatgatctg ttcgagctcg
     3061 aactgggtct tggtgaccac ttcctcgatg cgcgcgggat ggatgcgtcc atcctgcaga
     3121 agcttcagga gtgatctccg ggcgacttcg cgtcgcaccg ggtcgaatcc ggagatcgtc
     3181 acggcttccg gggtgtcatc gacgatgagg tcgacaccgg ttgcctgctc cagtgcacga
     3241 atgttccgac cttcgcggcc gatgatccgg cccttcatgt cgtcg
//
LOCUS       DASTMD010000754         1579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_247623, whole genome shotgun sequence.
ACCESSION   DASTMD010000754 DASTMD010000000
VERSION     DASTMD010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247623"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VFQ54_11175"
     CDS             <1..1097
                     /locus_tag="VFQ54_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165595.1"
                     /translation="LLNPNLGGQIGWLIVLAIIGLIVAWGGTRFWTMSRARTALVLWG
                     GWLITMSIFFSVAGFYHRYYLTTLSPAIAALVGIGIVALWRNHQAGGVRILLLPAALA
                     VTAVVQAHILGTYPVWRDRLMPSIGILTGVAIIGLLGRSVIAFSPWIPRVLVSLGLAA
                     VILTPATWSFITVRDATAGASMPLAGPEGDAYGLGNFGGGRFGGGGANRISSATGDDS
                     SSSSAVDPELAYLIANKGTAKWIVAVASSSDGDQIILETGEAVMSVGGFDGADDILSV
                     DAFNALVAKGEIRFYLGGGFGGFGRGGSSSAISSWVEETCRVVPESTIEAGAVSPDPA
                     TTTPGTSRIVDQFGGSTLYDCGGAATSSEN"
     gene            complement(1259..>1579)
                     /locus_tag="VFQ54_11180"
     CDS             complement(1259..>1579)
                     /locus_tag="VFQ54_11180"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEU0165596.1"
                     /translation="REDPVLSHNDRQHTRAIALSAQVVAIHRWWGTEHRLVVANLGHE
                     VDLALPDIKSLLTLRYRPWTLLFSTADPRFTTNARPPTISGIGGHRRIQLPARAAAIW
                     EIAG"
BASE COUNT          235 a          522 c          532 g          290 t
ORIGIN      
        1 ggctcctgaa cccaaacctc ggcggccaga ttggctggct gatcgtcctc gccatcattg
       61 gcctcatcgt tgcctggggc gggacgcggt tctggacgat gagccgagcc cggaccgccc
      121 tcgttctctg gggcggctgg ctgatcacga tgtcgatctt cttcagcgtg gcgggcttct
      181 accatcgcta ctacctgacg acgctctccc cggccatcgc ggccctcgtc ggaatcggca
      241 tcgttgcgct ctggcgaaac catcaggcag gcggcgttcg cattctcctc ctgccagcgg
      301 ctctcgcggt gaccgcggtc gtccaggcgc acatcctcgg cacatacccg gtgtggaggg
      361 atcgtctgat gccgtccatc gggattctca ccggggtcgc gatcatcggc ctgctcggac
      421 gtagcgtcat cgccttctcg ccctggatcc cgcgcgtcct cgtctcgctc ggcctcgctg
      481 cggtcatcct caccccggcg acgtggtcat tcatcacggt gcgagacgcg acggcgggcg
      541 cctcgatgcc gttggcgggt ccggagggtg atgcgtacgg tctcggcaat ttcggcggcg
      601 ggcgtttcgg cggcggcggt gccaatcgga tttcttcggc gaccggtgac gatagctcgt
      661 cttcgtctgc cgtcgatccg gagctcgcct atctgatcgc caacaagggc accgcgaagt
      721 ggatcgtcgc cgtcgccagc tcgagcgacg gcgaccagat cattctcgag acaggcgagg
      781 ccgtgatgtc ggtgggcggc ttcgatggcg ccgacgacat cctgtccgtc gatgccttca
      841 atgccctcgt ggcgaagggg gagatccggt tctacctggg aggtggtttc ggcggcttcg
      901 gtcgcggggg atcgagctcc gcgatcagct cgtgggtcga ggagacctgc cgggtcgtgc
      961 cggaatcgac gatcgaggcc ggcgcggttt ccccggatcc ggcgacgacc acacctggga
     1021 cgtcacgaat cgtcgatcag ttcggcggca gcacgctcta cgactgcggc ggcgccgcga
     1081 cctccagcga aaactgaggc gattgccaaa cattcggtcg aatcagacgc gatgtcccgc
     1141 accgcatccg ttcgcagggc gggaccgccg ctgccggacc gcagtccgga cggcccgtcc
     1201 cgcctagagt tcgcccagaa atcgtgctcc ccgtgacaat ggtttcgatc gccgcgacct
     1261 agccggcaat ctcccagatc gccgccgctc gtgccgggag ctgaatccgc cggtgaccgc
     1321 cgatccccga gatcgtcgga ggcctggcgt tggtcgtgaa gcgggggtcg gctgtcgaga
     1381 agagcagggt ccaggggcga tagcgaagcg tcagcaggga cttgatgtca ggcagggcga
     1441 ggtcaacctc gtgcccaagg ttcgccacaa cgaggcggtg ctccgtcccc caccaacggt
     1501 gaatcgcgac gacctgggcc gagagcgcga ttgcccgcgt gtgctggcgg tcgttgtgcg
     1561 agagaaccgg atcctcccg
//
LOCUS       DASTMD010000755         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2034004, whole genome shotgun sequence.
ACCESSION   DASTMD010000755 DASTMD010000000
VERSION     DASTMD010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034004"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /locus_tag="VFQ54_11185"
     CDS             complement(<1..1899)
                     /locus_tag="VFQ54_11185"
                     /inference="COORDINATES: protein motif:HMM:NF045093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEU0165597.1"
                     /translation="MTSANSISGAPFSSRVNNSATASWIWDAGVPSPRNAWRRFRRTF
                     ATHHTVESASLTITADSRYVAFVNGVRVGYGPVRGFPHRWFIDTWEIGHLLHPDAPNT
                     IAVHVLHFGLSTFADHRKRGGLLAQIAINDEFETRNIGTDGTWLVETPFGHDPRANRL
                     SCQLGFTEQFDNRHDDGDWTLPGYDDSAWSAATVIAAPGDGPWTKLVPRDIPPLEERV
                     VRPGSLSRLAFVRPTPISAAIDLRVQLSPESTDHANHVAYTGYLLTTLRLARDATVTM
                     TLPGPSFRDLGINVGGRWFPRAALADGAQDSHSLTVDLEAGDHLVAIDISGRDHGHTF
                     SLLLDADDPGAVSLATPLGDADAGTPFVTVGPLTGVAIGRVEKIFPEVPPIPEDIAAR
                     MQSLGDPSRLAEFGERVRPVPAALVSPVSLYGLAVHPAERVEIPVPAAMQAIAAGNAI
                     EIPVRPARDTELIFDLGREFSGFMTVEVEAPAGTVVDLYGFEYLKGDHREDTSGLDNS
                     LRYIARDGRQRYTSPTRRGLRWLQVTIRNAVPGARIHLHDVAVIESHFPVSRSGSFRS
                     SDARLNEIWEMSRRTVIACMEDTYVDCPAYEQVFWVGDSYSSSRFAAYLFGAEALTER
                     CLRLVPESA"
     gene            2088..>3172
                     /locus_tag="VFQ54_11190"
     CDS             2088..>3172
                     /locus_tag="VFQ54_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0165598.1"
                     /translation="MAGARPNIILFVADDHRGTALRHLGGTRVRTPMLDTLAAAGVSF
                     ARAHSQGSMHPAVCVPSRASLLTGRSIFASSADPTGLDFTAAQTIPAQLETFPQALRA
                     GGYHTHAVGKWHNDRVSFNRSFGSGRAIFFGGMSDHYHVPLHDYDPTGAYPETAVRYE
                     ERFSTDIFTDAAIDFLEDRRTSEPFFLYVAYTAPHDPRTPPPRWSPKASQIEIAPNVV
                     PIHPFDNGEMKVRDELISVAPRSISEVRNQLSAYYGMIAHLDHAVGRVMRILQARGMD
                     EHTVVIYTSDHGLAVGQHGLMGKQNLYQPSLRIPLVMAGPGVPQGEIDSSLAWHADTC
                     ATIRAMAGLGPDPNREGVDVLADPGRIG"
BASE COUNT          563 a          986 c         1067 g          556 t
ORIGIN      
        1 tgccgattcc gggaccagcc gcaggcaacg ttccgtcaac gcctcggcgc cgaagagata
       61 ggccgcgaag cgcgagctgc tgtacgaatc gcccacccag aagacctgct cgtaggccgg
      121 gcaatcgacg taggtgtcct ccatgcaggc gatgacggtt cgccggctca tttcccagat
      181 ctcgttcagg cgcgcgtcgc tcgagcggaa ggagccgctc cgcgagacgg ggaagtggct
      241 ttcgatgacg gcgacgtcgt ggaggtggat tctcgcgcca ggaacggcgt tgcggatcgt
      301 cacctgcagc cagcggaggc cgcggcgggt aggcgacgta tagcgctggc gaccgtctcg
      361 cgcgatgtag cgaagcgagt tgtcgagccc actcgtgtcc tcccggtggt cgcccttcag
      421 gtactcgaac ccatacaggt cgacgaccgt ccccgccggc gcctcgacct cgacggtcat
      481 gaagcccgag aactcgcgac cgagatcgaa gatgagttcc gtatcgcgag cggggcgaac
      541 cgggatctcg atcgcatttc ccgcggcgat tgcctgcatc gcggcgggaa cggggatctc
      601 gactcgttcg gccgggtgga ccgcgagacc gtagagactg accgggctga cgagcgcggc
      661 cgggactggc cgcacccgct cgccgaactc cgccagccgc gatggatcgc ccagtgactg
      721 catcctcgcg gcgatgtctt ccggaatcgg gggcacctcc gggaagatct tctcgacgcg
      781 cccgatggcg acaccggtga gcggcccaac ggtgacgaac ggggtcccgg catccgcatc
      841 accgagcggt gtggcgaggg acacggcgcc gggatcgtcg gcatccagca ggagactgaa
      901 cgtatgaccg tgatcgcgtc ccgagatatc gatcgcgacg agatggtcgc ccgcctcgag
      961 atcgacggtc aggctgtgcg aatcctgcgc cccgtccgca agcgcggcac gcggaaacca
     1021 gcgaccgccg acgttgattc cgagatcgcg aaagctggga ccggggagcg tcatcgtcac
     1081 ggtcgcgtct ctcgcgagcc gcagcgtggt caggaggtat cccgtgtagg ccacgtggtt
     1141 ggcgtgatcg gtgctttccg gggaaagttg cacccggagg tcgatcgccg cgctgatcgg
     1201 ggtggggcgg acgaaggcga ggcgactgag cgagccgggg cgaacgaccc tctcttcgag
     1261 cggtggaatg tcccggggaa cgagcttcgt ccacggaccg tcccctggcg cggcgatgac
     1321 ggtggctgct gaccaggcgg agtcgtcgta tcccgggagt gtccagtcgc cgtcatcgtg
     1381 gcgattatcg aattgctcgg tgaaacccag ttggcaggag agccgattgg cgcgaggatc
     1441 atggccaaat ggtgtttcga cgagccaggt gccgtcggta ccaatattgc gagtctcgaa
     1501 ttcgtcgttg attgcgatct gcgccagtag tccgccgcgc ttgcggtggt cggcgaaggt
     1561 ggagagcccg aagtggagaa cgtggacggc gatcgtattg ggggcgtcgg ggtggaggag
     1621 gtgaccgatc tcccaggtat cgataaacca gcgatgcgga aacccgcgga caggtccata
     1681 ccccacccgg acgccgttaa caaaggcgac gtatcgtgaa tccgcggtga tggtcagtga
     1741 cgcggattcg acggtatgat gtgtggcgaa cgttcgccta aagcgtcgcc aggcgttgcg
     1801 cgggctcggg actcccgcat cccagatcca ggaagcggtc gcagaattgt taacgcgaga
     1861 ggagaaaggc gcacctgaaa tcgagttcgc agatgtcata acgtgaactc ccttccgaag
     1921 tcggtagtca accttttgac caaataccag cgtattacgt gatgtggtca cggtatcccg
     1981 agtcgcatat tgacgcaagg aatcgcgatc acgatcctgt gggcacgcgc ttcccgcgcg
     2041 gctggggcca aggtcaaccg tcgtgtctct ccaggagtgg ctagtccatg gcaggcgccc
     2101 gcccaaacat cattctcttt gtcgccgacg atcatcgcgg cacggccctg cgccatctgg
     2161 ggggaacccg ggtgcgcacc cccatgctgg atacgctcgc cgcggcgggg gtttcgtttg
     2221 ctcgagcgca tagccaggga tcgatgcatc cggcggtctg cgtgccgagc cgggccagcc
     2281 tgctgaccgg gaggtcgatc ttcgcatctt ccgccgatcc caccgggctc gatttcacgg
     2341 cggcacagac gatccccgcg cagctcgaga ccttcccgca ggcgctgcgc gccggtggct
     2401 accacacgca tgccgtcggc aagtggcaca acgaccgcgt ctccttcaac cgctcgttcg
     2461 gcagcggcag ggccatcttc tttggcggca tgagcgacca ttaccacgtt ccgcttcacg
     2521 actacgatcc caccggagcc tatcccgaga ccgccgttcg ctatgaggaa cggttctcga
     2581 ccgatatctt cacggatgcc gcgatcgact ttctggagga ccggcggacc agcgagccgt
     2641 tctttctcta cgtggcgtat acggctccgc atgatccgcg gacaccgcct ccacggtggt
     2701 cgcccaaggc ttcgcagatc gagatcgcgc cgaatgtggt gccgattcac cccttcgaca
     2761 atggcgagat gaaggtgcgc gatgagttga tctctgtcgc gccgcgctcg atcagcgagg
     2821 tgcggaacca gctctcggcg tattacggga tgattgccca cctcgatcac gccgttgggc
     2881 gcgtcatgcg catccttcag gcgcggggca tggacgagca taccgtcgtg atctacacct
     2941 cggatcacgg cctggctgtc ggtcagcatg gactgatggg gaagcagaac ctctaccagc
     3001 ccagtctgcg gataccgctc gtcatggccg gaccgggggt gccgcagggc gagatcgatt
     3061 cctcgctcgc gtggcacgcg gatacctgcg ccacgatccg ggcgatggcg ggtctcgggc
     3121 ccgatccgaa ccgggagggt gtcgatgtcc tcgctgatcc ggggcggatc gg
//
LOCUS       DASTMD010000756         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_606818, whole genome shotgun sequence.
ACCESSION   DASTMD010000756 DASTMD010000000
VERSION     DASTMD010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606818"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VFQ54_11195"
     CDS             <1..1152
                     /locus_tag="VFQ54_11195"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165599.1"
                     /translation="GDWQSGLRDGAGVAMATFCHGTTEELWYMPRLDTLLNGQSSPTA
                     GGENREGNVNVLVLGRGDLKSLISMDEAIGLMKNAFQELSAGRAVVPLRTVIDVEPGT
                     SSTLVMPAYLPGLNALGFKVISIFTRNPERGLPTGNAMVCMLDEETGAPVALLNGAFL
                     TALRTGAVSGAATDLLARPDARVLTIIGAGVQGATQALAVCAVREIEKINVVRRSDAS
                     WHRFEALVERDWPELLPRMTGTTDVEAAVREADVICAATTSGTPVFEDAWVQPGTHIN
                     GVGSFTPETREIPGETIVRARVVVDHRESALSEGGDLIIPLNEGLIGLDHIVAELGEV
                     VAGDIPGRTTADEITFFKSVGNAVQDVVTARRAIELATARGVGQEISLD"
     gene            complement(1293..1958)
                     /locus_tag="VFQ54_11200"
     CDS             complement(1293..1958)
                     /locus_tag="VFQ54_11200"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU0165600.1"
                     /translation="MPDIIVNGGEWYAKMGTEGSKGTKLMTMQGPVKRHGVVEVEMGV
                     SFDTIFNDIFGGMRDGHTLKGVQTGGVSAGPLLEEHFDLPVDFDSLSGVGGMLGSGGF
                     VAFDDQVCAVDFARYLMAFNRYESCSKCVPCRLGNPALVEILDRIRFGRGSMDDLILI
                     KRTSKHITELSLCGLGQIAPAPLLGMMERFEEEFRQHIEDGICKTGTCPIAARAPELV
                     AAD"
     gene            complement(1965..2381)
                     /locus_tag="VFQ54_11205"
                     /pseudo
     CDS             complement(1965..2381)
                     /locus_tag="VFQ54_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
     gene            complement(2400..3023)
                     /locus_tag="VFQ54_11210"
     CDS             complement(2400..3023)
                     /locus_tag="VFQ54_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165601.1"
                     /translation="MVQTADGNMLPRLTSKEDFLAFEAGAKERWDDLWSGSRLVVSVG
                     VGSSSIAKGALEILTRCRREVGDGGVVREVSGNGAFWMEPWIEVKRPDAPPVVYGPVT
                     EDEVAAVIAGAREDLAVGVRGDETFGSIRPLGEHPFFSHQTSLLLRNSGVIEPESIED
                     AIARGAYRAYFQVLFEMSGEEAIAEVTASQVRGRGGGGFPAGIKWES"
BASE COUNT          542 a          982 c         1024 g          557 t
ORIGIN      
        1 ggtgattggc agtctggcct ccgcgacggt gcgggcgtcg cgatggcgac tttttgtcac
       61 ggaacgacgg aagaattgtg gtacatgccg aggctggata cactcttgaa tggacaaagc
      121 agccccacgg ctggcggcga aaatcgtgaa gggaatgtca acgtgctggt gctcggacga
      181 ggcgatctga aaagcctgat ctccatggat gaggcgatcg gtctcatgaa gaatgccttc
      241 caggaactct ccgccggtcg tgcagtcgtg ccgctccgga ccgtcatcga cgtcgaacca
      301 ggcacgtcgt cgacgctggt gatgccggcc tatcttcccg gattgaatgc gctcggattc
      361 aaggtgatct cgatcttcac ccggaatccg gaacggggcc tcccgaccgg caacgcgatg
      421 gtctgcatgc tggacgagga gacgggcgcg ccagtcgccc tgctcaacgg tgcgttcctc
      481 acggcgctac gcacgggcgc ggtctctggc gccgcgacgg accttctggc gcgtcccgac
      541 gctcgtgtcc tgacgatcat cggcgcaggc gtgcaggggg cgacccaggc gctggccgtt
      601 tgcgccgtcc gcgagatcga gaagatcaac gtcgtgcggc gctcggatgc gtcgtggcat
      661 cggttcgagg cgttggtgga acgtgactgg cccgagttgc tgccacgaat gacggggacg
      721 acggacgtcg aggcggcagt gcgggaagcg gatgtgatct gcgcggcgac gacatcggga
      781 acgccggtct tcgaggacgc ctgggttcag cccggaaccc acatcaacgg cgtgggttcg
      841 ttcaccccag agacgcgcga gatcccgggc gagaccatcg tccgcgcgcg cgtggtcgtc
      901 gaccatcgcg aatcggcgct cagcgagggg ggcgacctaa tcatcccgct caacgaggga
      961 ctgattggcc tcgatcacat cgtggcggaa ctcggggaag tcgtagcggg ggatattccc
     1021 ggtcggacga ccgcggacga gattacgttc ttcaagtccg tcggcaatgc cgttcaggac
     1081 gtcgtgaccg cgcgacgggc gatcgaactc gcgacggcac gaggggtggg ccaggagatc
     1141 tcgctcgact gatcgcgatt cccgattacg aacttcggga attggcaggc gtagacgagg
     1201 agggcggcgg ctttgtcgcc gccctcctcg tctacgccgg tatccggatg cttcgcccca
     1261 taggacatcc ggtcggtcgt tcgatcgttc ctctagtccg ccgcgacgag ttcgggtgct
     1321 cgagccgcga tcgggcaggt gccggtcttg cagatgccat cctcgatgtg ctggcggaac
     1381 tcttcctcaa agcgctccat catgccgagc agcggggcag gcgcgatctg gccgaggccg
     1441 cacagcgaga gttccgtaat gtgcttgctc gtgcgcttga tcaggatcag atcgtccatc
     1501 gagccgcgcc cgaaacgaat gcggtcgagg atctcgacga gcgccgggtt ccccaggcga
     1561 cagggcacgc atttcgaaca cgactcgtag cggttgaacg ccatcaggta gcgggcgaag
     1621 tcgacggcgc agacctggtc gtcgaaggcg acgaagccgc cggaaccgag catcccgcca
     1681 acgccgctga gcgagtcgaa gtcgaccggg aggtcgaaat gctcctcgag cagtgggccg
     1741 gcggagacgc ctcccgtctg gacgcccttg agcgtatggc cgtcgcgcat cccgccgaag
     1801 atgtcgttga agatcgtgtc aaaggagacg cccatctcca cctcaacgac accgtgccgc
     1861 ttgaccgggc cctgcatcgt catcagcttg gtgcccttgc tgccttcggt gcccatcttc
     1921 gcgtaccact caccgccgtt gacgatgatg tccggcatgt tggcaaacgt ctcggtgttg
     1981 ttggcgatcg tcgggcgctt ccagaggccc tcctcgaccg agcgcggtgg cttggtgcgt
     2041 ggctgaccgc ggacgcccat cacggagtac atcagcgccg acgcctcgcc gcagatgtag
     2101 gcgccgccgc cggtgcgaac gcggaacgca acgttcttgc ccgatcccag gacgttcttg
     2161 ccgatcaggc cgagccgctc ggcatcggcg acggcccggc ggaaacgctg gatcgccagc
     2221 ggatactcgc caccgatgta gttgtagcca cgctcggcgt cacaggtcac gcagcagatg
     2281 agaatcccct cgaggattcg atgggggtcc gcctcgtgaa ggtgccgatc cttgtaaacg
     2341 ttcggctcgc cctcgtggct gttgcagacg acgaacttcg gcttgttccg ggcctttcgt
     2401 cagctctccc atttgatgcc ggcggggaag cctccgcccc cacgtccgcg aacctgggag
     2461 gcggtgacct cggcgatcgc ctcttcaccg gacatctcga agagcacctg gaagtacgcc
     2521 cggtatgcac ctcgcgcgat cgcgtcctcg atcgactccg gttcgatgac accggaatta
     2581 cgaagaagga gcgatgtctg gtggctgaag aaaggatgtt cgccgagtgg gcggatggaa
     2641 ccgaacgtct cgtcgccgcg gacgccaacg gcgagatctt cccgggcgcc ggcaatcacc
     2701 gccgcgactt cgtcttccgt gacaggtccg tagacgacgg gcggtgcgtc cggtcgcttc
     2761 acctcgatcc acggctccat ccagaaggcg ccgttcccgc tgacttcacg gacaacgccg
     2821 ccgtcgccga cttcacgccg gcaccgcgta aggatctcga gtgcaccctt ggcgatcgag
     2881 ctgctgccga cgccgacgct gacgacgagc cgggaaccag accagagatc gtcccatcgt
     2941 tctttcgccc cggcctcgaa cgcgaggaag tcttccttcg atgtcagtcg agggagcatg
     3001 ttaccgtccg ccgtttgaac catgggatcc ctcctgggcc tggcgcgcgc tgcgaccggc
     3061 gatggcatcg gcgagcagac ggttcaagta ttccgggctg acatt
//
LOCUS       DASTMD010000757         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1857557, whole genome shotgun sequence.
ACCESSION   DASTMD010000757 DASTMD010000000
VERSION     DASTMD010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857557"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VFQ54_11215"
     CDS             <1..1037
                     /locus_tag="VFQ54_11215"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEU0165602.1"
                     /translation="KETRDRTCAGTVIGCSGIGVAGNGLELEHRPALSLMAFWLPGAK
                     IRPVRIHQENLSLLSDPELWTSLSGVEPADVTSWVVLGDPFRHDVQILLDGLSDLYPG
                     IPVVGGLTSSPRDDRRGWVFLDDRVYDEGGIALAIGGPYRIEPICSHGCDPIGETWTV
                     TGAEKNVLLTISGRPALDVMNDTLKSVPSEKRAAAIENLVVGFAADEYRDEFHRGDYL
                     VRGVIGVDEARGSITVGGKPRCGQTVQFHIRDSEVADIDLEQALTDARALLAGSRPVA
                     GLLFACTARGSALFGSSHHDARALQLAFPLMPAAGTVVCAEIAPAGRRIGIHGLTATI
                     GLLVHDDLVG"
     gene            1121..1615
                     /locus_tag="VFQ54_11220"
     CDS             1121..1615
                     /locus_tag="VFQ54_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0165603.1"
                     /translation="MNLAELHYVGKMLMECATNGMQALGKESLTATELVVAETLYVLG
                     PVSIGDIARRTGFVQSRISTVVADLHERGIVRVYTDGRDRRRTIVEISEASLAEVARV
                     SAGDAFPALTTWFPNATSSQVDALVDALSGAIASFDPATTTGANGATGILSKRQVIVR
                     TPAT"
     gene            complement(1651..2241)
                     /locus_tag="VFQ54_11225"
     CDS             complement(1651..2241)
                     /locus_tag="VFQ54_11225"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU0165604.1"
                     /translation="MYDETLTRCGWPGSDPLYVKYHDEEWGVPVRDDAELFERLMLEG
                     FQAGLSWITILRKREAFHAAFDGWDAEKIVNYGEDDIARLLNDPGIVRNRLKVNGAVR
                     NARAYLQVRDELGSFSDYLWDFVGGQPLVNRPATLADIPPISPEATAMSKALKKRNFT
                     FVGPTIIYAFMQSVGMVNDHVVGCFCLEQMDASIVV"
BASE COUNT          439 a          775 c          812 g          470 t
ORIGIN      
        1 tcaaggagac ccgggatcgt acctgcgccg ggaccgtcat cggctgttcc gggatcggtg
       61 tggcggggaa tggactcgag ctggagcacc gtcccgcact ctcgctgatg gcgttctggt
      121 tgccgggcgc gaagatccgc cccgttcgca tccaccagga gaacctctcg cttctttcgg
      181 atcccgagct ctggacgtcg ctctctggtg tcgagccggc cgacgtcacc tcctgggtcg
      241 tcctggggga tcccttccgt cacgatgtcc agatcctgct cgatggcctg agcgatctct
      301 atcccggtat ccccgttgtg gggggactca cgtcgagtcc acgtgacgac cgtcgcggtt
      361 gggttttcct cgatgaccgc gtctacgacg agggaggaat cgcgctggcg atcggcgggc
      421 cctaccgcat cgagccgatc tgctcccacg gctgcgaccc gatcggcgag acgtggaccg
      481 tgaccggggc cgagaagaac gtgctgctca cgatctcggg acggccggcg ctggacgtga
      541 tgaacgacac gctgaagtcg gttccgagcg agaagcgggc agccgccatc gagaacctcg
      601 tcgtcggatt cgccgccgat gagtaccggg acgagttcca tcgcggggac tatctcgttc
      661 ggggggtgat cggcgtcgac gaggcacgtg gctcgatcac cgttggcggg aagccgcgat
      721 gcggccagac cgtgcagttc cacatccgtg attctgaagt cgccgacatc gatctcgaac
      781 aggcgctgac cgacgcccgc gccctcctgg ccggaagcag accggtggcg ggattgctct
      841 tcgcgtgcac ggcgcgagga tcggcgctct tcggatcgtc gcatcacgac gcccgcgcgt
      901 tgcagctcgc gtttccgctg atgccggcgg cagggacggt cgtctgcgcg gagattgcgc
      961 cggctggacg ccggatcggg attcacggcc tcacggcaac gatcggcctg ctcgtgcacg
     1021 acgacctggt tggctgagat gcatcgtctc gaatgcatca ttggtgatgt acactcccca
     1081 gcattctgag acatgtgacc ttccgggggg tgggaaaaag atgaatctgg ctgagctgca
     1141 ttacgttggc aagatgctta tggaatgtgc gaccaacggg atgcaggcgc tcggcaagga
     1201 gtcgttgacg gcgacggaac tggtggtcgc cgaaacactc tacgtgcttg gtccggtttc
     1261 gatcggtgat atcgcgcggc gaacgggatt cgttcagagc cggatctcga ccgtggtcgc
     1321 ggatctccac gagcgaggca tcgtccgcgt ctataccgac gggcgtgacc ggcggcgcac
     1381 gatcgtcgag atcagcgagg cgtccctggc cgaagtcgcg cgggtgagcg ccggcgatgc
     1441 gtttcctgca ctcaccacgt ggtttcccaa cgcaacgagc agccaggtcg atgccctcgt
     1501 ggacgccctg agcggggcga tcgcctcgtt cgaccctgcg acgacgacag gtgcgaacgg
     1561 ggcgacggga attctgagca agcgtcaggt gattgtccga acgccggcaa cctgatcgca
     1621 gatggcggct ggagcatatt ccagccgtgc ctacaccacg atcgaggcgt ccatctgctc
     1681 caggcagaaa catccgacaa cgtgatcgtt gaccatgccc acgctctgca tgaaggcgta
     1741 gatgatggtg ggtccgacga aggtgaagtt gcgcttcttg agcgccttcg acatcgccgt
     1801 cgcttccggg ctgatcgggg ggatatccgc gagggtcgcc ggtcggttga cgagcggttg
     1861 cccaccgacg aaatcccaga gatagtcgct gaatgatccg agttcgtccc tgacctggag
     1921 ataggcgcgg gcattgcgca cggcgccatt caccttgagc cggttacgca cgatgccggg
     1981 atcgttcagc agacgggcga tgtcgtcttc cccatagttc acgatcttct cggcatccca
     2041 accatcgaac gcggcgtgga acgcttcccg tttgcggagg atggtgatcc agctgagacc
     2101 ggcctggaat ccctcgagca tcagccgctc gaagagttcg gcgtcgtcgc gaacggggac
     2161 gccccattcc tcgtcgtggt acttgacgta aagcggatcg cttcctggcc atccacaccg
     2221 cgtcaatgtt tcgtcgtaca tgcgaactca atcccccggt agatgcgtcc atcccgatgg
     2281 aaggagtcta gggaagaaca catgttcgcg tcaagtcctg ggactcgttc attcgatggc
     2341 cgggagatta tccgcaccac gtcattccgg gtccgaacgt cagctgagcc cagatgtcct
     2401 cgcgagcccc gaacgtcacc ggcgagcccc ggacacgtcc tcccgagcct gtcgagggac
     2461 cacgccggca ccacgagctt tcggagcaac gcctcc
//
LOCUS       DASTMD010000758         4212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3014966, whole genome shotgun sequence.
ACCESSION   DASTMD010000758 DASTMD010000000
VERSION     DASTMD010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3014966"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="VFQ54_11230"
     CDS             <1..1563
                     /locus_tag="VFQ54_11230"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEU0165605.1"
                     /translation="FLATFDETQLAATQLDVDDDIFRQWTNVDGTDRQGIGFRAMNDD
                     QKTAAYALLKASLSAYGYETAGNVMKLNHTQGELTNDFERFDEDLYFFTIYGTPSLTD
                     PWAWRIEGHHLIICSFILGDQLVMTPTFLGSEPAIAPVDTDYAGLSVLQDEQQAGHDF
                     INALSAAQQASAILSSEKTKENLEAGAYSDNATIPYAGIQASTLDATLQKNLLDLIAL
                     WVSHMPDDQAAIKMAEVKDHLGDTWFAWIGKTDLDAVFYYRVQSPVILIEFDHQPPGP
                     LGQESDYYNGATGPQRMHIHSIIRSPNGGDYGKDLLAEHYATSSHHTATPVATPESSP
                     VADTGLVLTGAGIAVAAIREILPPRYRNGRAVSNMPMGNPAGLVYDAEGKVAWDCIWE
                     DFCDLALAGGPPHRETMLQAPGHDAVLADMASYLRVVAEIRRGLTMVTGWDARPASSF
                     GWVDLACPDEETAIWMQQAILAENIAVIRHGTTIALPAGPAFTLEKEIRNVITSVAKT
                     HHYWTEHLLAIA"
     gene            complement(1644..2657)
                     /locus_tag="VFQ54_11235"
     CDS             complement(1644..2657)
                     /locus_tag="VFQ54_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEU0165606.1"
                     /translation="MPDGALILLILIVLLGLTFDFINGFHDTANAIATVVATRVLTPG
                     QAVLMSVVLNIVGAMTGQAVAATVGKGIIETSFVTQELVIAALIAAITWNLITWWFGI
                     PSSSSHALIFSLVGAGIAAAGTHVIQGSGVTKTLQGMIYSPLLGFAIAFLVLIGLLWI
                     VARRRPRTVNRVFGRLQIFSAAFMAYSHGSNDAQKTMGVITLALASYHGWSGSEWNVP
                     LWVTLSAAGAMGLGTATGGWRIVKTMGLKVVELRPIDGFAAETSAAAIIEVASRLGIP
                     VSTTHVISSAIMGVGATRRASAVRWGIAGRILVAWVVTIPVCIAIGWAVFQVIDAGKS
                     LFS"
     gene            complement(2650..3294)
                     /locus_tag="VFQ54_11240"
     CDS             complement(2650..3294)
                     /locus_tag="VFQ54_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEU0165607.1"
                     /translation="MVLSRFLPKDEQFFDFFREAAENASESARLLASILAIDGDESAR
                     NVRRLRDLEHRGDEISHRIFSALNSTFVTPLDRDDIRELATTIDDFVDGIEECGKRFS
                     LYRLEVATPMAQQFAQILVEQGVAILAAVPLLAQAAKHAQDLRRWVLELHRLENEADD
                     LLNNALASLYDGVDDVPGLIHAHRWGELYQMLEDATDCGEDVADVLEGILITYA"
     gene            complement(3380..3829)
                     /locus_tag="VFQ54_11245"
     CDS             complement(3380..3829)
                     /locus_tag="VFQ54_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0165608.1"
                     /translation="MNEQQPIRIESLDHLVLTVRDLPTTVAFYTRVLGMRLVRFGEGR
                     LALEFGSQKINLHRMGHEYEPKSAHPTPGSADLCFILASRLSDVVVHLRGLGVTVEDG
                     PVTRVGALGPMESIYLRDPDGNLIELSNYVDDDGDAEAGLDRPPILR"
BASE COUNT          832 a         1400 c         1262 g          718 t
ORIGIN      
        1 ttccttgcca ccttcgatga aacgcagctt gccgcaaccc agctcgacgt cgacgacgat
       61 atcttccgcc agtggaccaa tgtcgatggc accgatcggc agggcatcgg ctttcgggcg
      121 atgaacgacg atcagaagac cgccgcatac gcactgctca aggcgtcact cagcgcatac
      181 gggtatgaga cagccggaaa tgtgatgaag ctcaatcata cccagggcga attgacgaac
      241 gacttcgagc gcttcgacga ggacctctac ttcttcacga tctacggcac gccatcgctt
      301 accgacccct gggcctggcg aatcgaaggt catcacctca tcatctgctc cttcattctc
      361 ggggatcagc tcgtgatgac gccgacgttc ctgggatccg aaccggcgat cgcacccgtc
      421 gacaccgact acgcgggcct ttccgtgctc caggacgagc agcaggcagg acacgatttc
      481 atcaacgcgc tttcagcggc gcagcaggca tcggccatcc tcagctccga gaagacgaag
      541 gagaatctcg aggcgggcgc atactccgac aatgccacga ttccctatgc cgggatccag
      601 gcttcgacgc tcgacgccac gctccagaag aacctgctcg atctcatcgc cctctgggtc
      661 agccatatgc cggacgatca ggcggcaatc aagatggcgg aggtgaagga tcatctgggc
      721 gatacctggt tcgcctggat cggcaagacc gatctggacg ccgtcttcta ctaccgcgtc
      781 cagagtccgg tcatcctgat cgagttcgat catcagcccc ccggcccgct cggtcaggaa
      841 tccgactact acaatggcgc gaccggtccg cagcggatgc acatccactc gatcatccgc
      901 tcccccaatg gcggcgacta cggcaaggac cttctcgccg agcactacgc gacgagttcc
      961 caccacactg ccaccccggt ggcgacgcct gaatcatctc ccgtggctga taccggactc
     1021 gtcctcaccg gtgcgggtat cgccgtggcc gcaatccgcg aaatactccc gccccgatac
     1081 cggaatgggc gtgccgtcag caacatgccg atgggaaatc cggccgggct cgtctacgac
     1141 gccgagggga aggtcgcctg ggattgcatc tgggaagatt tctgcgacct cgcgctcgcc
     1201 ggcgggccgc cgcaccgcga gacgatgttg caggcaccgg gtcatgacgc agtgctcgcc
     1261 gacatggcgt cgtatcttcg cgtcgttgcc gagatcaggc gcggactgac gatggtgacc
     1321 ggatgggacg cgcgtccggc gtccagcttc ggttgggtcg atctcgcctg tcccgatgaa
     1381 gagaccgcga tctggatgca gcaggcgatc ctcgccgaga acatcgcggt catccgccac
     1441 ggaaccacga tcgcactccc ggccggtccg gccttcacgc tggagaagga aatcaggaac
     1501 gtgattacct cggtcgcgaa gacgcaccac tactggaccg agcacctgct cgcgatcgcg
     1561 tagcgcgtcg cgatacgccg gccagggtgt ccacgccagc ccgccgcaat gcctcactgg
     1621 aaagcggtgg gccggcgtgg atctcaggag aagagagact tccccgcgtc gatcacctgg
     1681 aagacggccc agccgatcgc gatgcagacc ggaatcgtca ccacccacgc caccaggatc
     1741 cggccggcaa tgccccaccg cacggcggat gcgcgccgcg tcgccccgac ccccatgatc
     1801 gccgacgaga tgacgtgcgt cgtcgagacc gggataccga ggcgactcgc gacttcgatg
     1861 atcgccgccg ccgaggtctc ggccgcgaag ccgtcgatcg ggcgcagttc cacgaccttg
     1921 agccccatcg tcttcacgat ccgccagcca ccggtggccg ttcccagccc catcgccccg
     1981 gcggcggaga gtgtcaccca gagcgggacg ttccactccg aaccgctcca gccgtgatag
     2041 ctggcgagcg cgagcgtgat cacgcccatc gtcttctggg catcgttgct tccgtggctg
     2101 taggccatga aggccgcgga aaagatctgg agtcgcccga agacccggtt caccgtgcgc
     2161 ggtcgtcttc gcgcgacgat ccagaggagc ccgatcagga cgaggaaggc gatcgcgaag
     2221 ccgaggagcg gcgaatagat catgccctgg agcgtcttcg tcacgccgct cccctggatc
     2281 acatgcgtcc cggcggcggc gataccggcg ccgacgagcg agaagatcag ggcatgactc
     2341 gagctggagg gaatgccgaa ccaccaggtg atcaggttcc aggtgatcgc cgcgatcaaa
     2401 gccgcgatca cgagttcctg cgtcacgaac gaggtctcga tgattccctt gccgaccgtc
     2461 gccgcgaccg cctgccccgt catcgccccc acgatattca gcaccacgga catcaggacg
     2521 gcctgtcccg gcgtcaggac acgggtcgcg acgacggtcg cgatcgcgtt ggccgtgtcg
     2581 tggaagccgt tgatgaaatc gaaggtcagg ccgaggagga cgatcaggat caggaggatc
     2641 agcgcgccgt caggcatagg tgatcaggat cccctcgagg acatcggcca catcctcacc
     2701 gcaatcggtc gcatcctcga gcatctggta gagctcgccc caccggtggg catggatgag
     2761 cccagggaca tcgtccacgc cgtcgtagag actggcgagc gcattgttca gcaggtcatc
     2821 cgcctcgttc tcgaggcgat gcagttccag cacccatctc cgcaggtcct gcgcgtgctt
     2881 cgcggcctgc gcgagcaatg gcacggcggc aaggatcgcc acgccctgct cgacgaggat
     2941 ctgggcgaac tgctgtgcca tcggcgttgc cacctccagc cggtagagcg agaagcgttt
     3001 gccgcactcc tcgatcccat cgacgaagtc atcgatcgtc gtcgccagtt cgcggatgtc
     3061 gtcgcgatcg agcggcgtga cgaaggtgct gttgagcgcg ctgaagatac ggtggctgat
     3121 ctcgtcaccc cgatgttcga gatcgcgcag acggcgcacg ttgcgtgcgc tctcgtcacc
     3181 atcgatcgcc aggatcgagg caagcaggcg cgcagattcc gaggcgtttt ccgcggcttc
     3241 cctgaaaaag tcgaagaatt gctcgtcctt cggcaggaac cgggacagaa ccatgcgcac
     3301 tatccttcgt tggctggaga aaccggcatc gatcgtgaca tccaccagcg atgccactcg
     3361 tgaaatcaat aacgcaatat taacggagta tgggcggacg gtcgaggccg gcctctgcgt
     3421 cgccatcatc atctacgtaa ttggataatt cgatcaggtt cccatcgggg tcgcgcagat
     3481 agatcgattc catcggcccg agcgcaccga cacgcgtcac cggcccatcc tcgaccgtca
     3541 cgccgagccc ccgcaggtgg acgacgacgt cggacaggcg cgacgcgagg atgaaacaga
     3601 ggtcggcgga acccggcgtc gggtgcgcgg atttcggttc gtactcgtgt cccatccggt
     3661 gcaggttgat cttctgggag ccgaattcga gcgccagccg cccttccccg aatcgcacca
     3721 atcgcatccc gagcacccgc gtatagaaag cgaccgtcgt cggcaggtcc cgcaccgtca
     3781 ggaccagatg atcgagggat tcgatccgga tcggctgttg ctcattcacc gcatgacccc
     3841 atccgcttat gacaaccaaa ggagagcgct cgcctattct ggcacggctc cgtgcaggtg
     3901 ttcccggaac tcgtccgaaa ttggttcaca aaaagcatag acagctggcg gaaccgcgag
     3961 tagagtgtgc cgccaaacga caaagatata tccgtccgat gcgttccgat ttcatcgcga
     4021 tcaaaggacc cacatgacac agactcctgt ccgtctcggc attgtcggag ccgcccgcat
     4081 tctcaatgcc cacatgcgcg gcatcaagcg catccgggat gccgggcttg ccgatctgcg
     4141 catcacggcg atcaccgccc gccggctcga tgacgccgtc accttccgcc ttcgtggtga
     4201 aggtccggcg cc
//
LOCUS       DASTMD010000759         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1500538, whole genome shotgun sequence.
ACCESSION   DASTMD010000759 DASTMD010000000
VERSION     DASTMD010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500538"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VFQ54_11250"
     CDS             <1..1015
                     /locus_tag="VFQ54_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165609.1"
                     /translation="AYQLGYPPETEIDGCSVGTYVGVLRALIGLARRSWNEGEEVLFH
                     SEDSLVSGMASGLGLEPETARRAISAFTFDRENAGYHAAVAGIAAAPLVRGDRGSIAF
                     SRCGLTAQPLLFMTRELRRRAAENYHNVAHRREAMFRQDLYGLFGDRRFVASPGRIAL
                     RRENGNLRTDIDAAIFDRKTGTLGLFELKSQDPFARSMAELNRQRDSVLYANHQVSGV
                     LDWINRHGAGEILERVDRQTAKRFRVQKVYPFVLGRYLVHFNDGPQPDRRAVWATWPA
                     VLRMTSSSPFSGSETNPIASLFARLPQDVSIERPDPATPPRRISVGDLTLVVHGSRAE
                     FEG"
     gene            complement(1064..1693)
                     /gene="plsY"
                     /locus_tag="VFQ54_11255"
     CDS             complement(1064..1693)
                     /gene="plsY"
                     /locus_tag="VFQ54_11255"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU0165610.1"
                     /translation="MDAVGGIALIIVAYLLGAIPWGVVLGRVFAGVDVREHGSQSTGA
                     TNALRVLGKKFSIAVLILDMAKGILPVVVGRVVGLPEWAIGIAAVAAVVGHCWSPYIG
                     FSGGKGVATGGGAAVALFPWLLILFPVMIVIVYLTRYVSLASIVAAVIGAIVAIVTAA
                     LGGLPWWWGIAIGVIAAIMLERHKGNISRLRSGNENKFGARVTPRAETP"
     gene            complement(1694..>2019)
                     /locus_tag="VFQ54_11260"
     CDS             complement(1694..>2019)
                     /locus_tag="VFQ54_11260"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU0165611.1"
                     /translation="GQRVSSVLETALTILEEREKRISTSALNKMLQDAVAKHQPPSKP
                     GKWVKFFYATQADVAPPTFIIFCNDPKAIHFGYRRYLENELREQFGFKGTPIRISFRG
                     RHENL"
BASE COUNT          372 a          648 c          644 g          355 t
ORIGIN      
        1 ggcctaccaa ctgggctatc ccccagaaac ggaaatcgat ggctgttctg tcgggaccta
       61 tgttggcgtc ctgcgggcgc tgatcggcct ggcgcgtcga tcctggaatg agggggaaga
      121 ggtcctcttc cactccgagg attcgctggt ttcggggatg gcctccggcc ttggactcga
      181 gcccgaaacc gcacgccggg ccatttcggc gttcaccttc gatcgggaga atgccggcta
      241 ccacgcggcc gtggccggga tcgccgccgc gccgctggtg cgcggcgatc ggggatcgat
      301 cgcgttctcg cggtgcggat tgaccgcgca acctctgctc ttcatgacgc gggaactgag
      361 gcggcgagcc gcggaaaact accacaacgt cgcccaccgc cgggaggcga tgttccgtca
      421 ggatctctac ggtctcttcg gggacaggcg atttgttgca agccctggtc gcatcgcgtt
      481 gcgtcgtgaa aacggcaacc tgcggaccga catcgatgcg gcgatctttg accggaagac
      541 cgggactctc ggtctcttcg agttgaaatc ccaggacccg tttgcccgtt ccatggcgga
      601 gttgaatcgc cagcgcgaca gcgtcctcta tgcgaaccac caggtctccg gcgtgctgga
      661 ctggatcaac aggcacgggg ccggcgaaat cctcgagcgg gtcgatcgcc agacggcgaa
      721 gcggttccgc gttcagaagg tctacccctt cgtcctcggt cgctacctcg ttcacttcaa
      781 cgacggaccg cagcccgatc gtcgcgcggt atgggcgacc tggcccgcgg tgcttcgcat
      841 gacctcgtcg tcaccgttct cgggctccga gacgaacccg attgcctcgc tgttcgcccg
      901 gctgccccag gacgtatcaa tcgaacggcc ggacccggcc acgcctccac gacggatcag
      961 tgtcggtgat ctgacgctgg tcgttcacgg gtcccgcgcg gagttcgagg gatagcgggc
     1021 ggcgccggag aatcggcggc agggaattgg gggtttgtgg tcgttacgga gtttcggcgc
     1081 gaggggtaac ccgagcgccg aacttgtttt catttcccga tcgcaaacgg gatatgttgc
     1141 ctttgtgtct ctcgagcatg atcgccgcga ttaccccaat cgcgatcccc caccaccatg
     1201 gcaatccacc gagcgccgcc gtcacgatcg caacgatcgc gccaatcacg gcggcgacga
     1261 tggacgccag agaaacgtac ctggtcagat agacgatcac gatcatcacg ggaaagagga
     1321 tcagcagcca ggggaagagc gccacggcag caccgccgcc ggtcgcgaca cccttgccac
     1381 cggagaatcc gatgtagggt gaccaacagt gaccgacgac cgccgccacg gccgcgatgc
     1441 cgatcgccca ctccggcaac ccgaccacgc gtccgacgac gaccggcaga atgcccttgg
     1501 ccatgtccag gatcaggacc gcgatcgaga acttcttgcc gaggacacga agcgcgttcg
     1561 tggcgcccgt cgattggctg ccatgctcgc ggacatcgac gccggcgaag acgcgaccga
     1621 gcacgactcc ccagggaatc gcaccgagca ggtaagcaac gatgatgagg gcgataccgc
     1681 cgaccgcatc catctagagg ttctcgtggc ggccgcggaa cgagatgcga atcggcgttc
     1741 ccttgaaacc gaactgctcg cgcagctcgt tctcgagata gcgacgatag ccgaagtgga
     1801 tcgccttggg atcgttgcag aagatgatga acgtcggcgg cgccacgtcg gcctgggtcg
     1861 cgtagaagaa cttgacccac ttgcccggct tggatggcgg ctggtgcttg gcgacggcat
     1921 cctgcagcat cttgttcagg gccgaagtac tgatgcgctt ctcccgctcc tcgaggatcg
     1981 tgagagcggt ctccagcacg ctgctgaccc gctgaccga
//
LOCUS       DASTMD010000760         4211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1766445, whole genome shotgun sequence.
ACCESSION   DASTMD010000760 DASTMD010000000
VERSION     DASTMD010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766445"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFQ54_11265"
     CDS             <1..880
                     /locus_tag="VFQ54_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165612.1"
                     /translation="HLDRVETSLMALARHELGENARFVGGDSFILWASPDRASHVPLG
                     RYELPRRNPDAYIFRLGHPLADFLIAQAKNRSLSTAHIVFDYTAYERKISSIERLVGT
                     SGWMSVSLLTLDGSEVTEDHVAVAAIRDDLQPVPAEDAMRFFTLPGYIGEDAPSPSSF
                     ISDLRIQAESVTNDIVAGAVERNAEYLEVESEKLDKWADDLRQSLRGELRSLEELIGE
                     TRRQIKITGLASERNAVRRRINQLEAELDEKRNELSRRSQEITRRRDDFYDDVDRRLR
                     QESTIHPLFTIRWSVI"
     gene            complement(889..2574)
                     /locus_tag="VFQ54_11270"
     CDS             complement(889..2574)
                     /locus_tag="VFQ54_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0165613.1"
                     /translation="MSRLISENLTLSYGEQTIVKDLSLDIQDGEITTIIGPNGCGKST
                     LLRGLARLMRPDSGQVHLDGQLIHSIPTREVAKRLGLQSQQALTPQGIMVEDLVRRGR
                     YPHQSFFQPPSQKDEEAIDRALALTGMESLRKRAVDQLSGGQRQRAWMAMAVAQDTPI
                     MLLDEPTTYLDVSHQLEIIDLVQHLNEREGKTIVMVLHDINEAARVSHRIVAMRDGEI
                     LRSGTPDEVLQPDLLARLYGVECDIYPHAARDCVFCMPRSSGSQAFDERSKPAATAGE
                     FSVAGLKTGYGKNVIIEDLSLELPAGKMIAIVGPNACGKSTLFRSCARLLKPMGGHAR
                     LDGKAVGSGSHKALAKRMSLLTQGPVPPSGFSVEDLVASGRVPHQSIFRQWSREDEGI
                     IDEAIDRCNLVEFRHREVDTLSGGQRQRAWFGMALAQDTPVLLLDEPTTFLDMAAQID
                     LLDMARQLNREKGYSVLMILHDLNMAARYADLIVAMKDGAVVATGTPDEVLTTDLLRD
                     VFEIEAAVSADPITGAVMIMPERHVSPHLGTAPTDDRFATDIRITSEPLQIAV"
     gene            complement(2580..3662)
                     /locus_tag="VFQ54_11275"
     CDS             complement(2580..3662)
                     /locus_tag="VFQ54_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0165614.1"
                     /translation="MATLDLATPGANQTAPGFHKPGHVTVRTPWLQVRFNVRVLGMSL
                     FVFGLLAVLAVFAMTLGSYRIPFVDVVKSVFGHGNDDQEFIVRTLRLPRLICAMLIGA
                     ALAMAGAIFQGLVRNPLVSPDIIGIDTGATLVAVFWIVFGFNSQWLPLGAFIGAVSAA
                     MLVYLLSWKRGINPNRLILVGIGVGAAISAGTTFITVRFPIDQVRPAIVWTMGSIYGS
                     NWTDVKTLLGFVIVCLPIGVGMMWHLRALQLGDDAARGLGIPIERTRLGLMLVGCGLA
                     AVTVSVAGPIGFVALMVPHIARMLGGALSSSVLVLTALLGAFFLLAADTVAQHFLPVA
                     LPVGVVTSAVGAPYFLFLLYRSNVRM"
     gene            complement(3665..>4211)
                     /locus_tag="VFQ54_11280"
     CDS             complement(3665..>4211)
                     /locus_tag="VFQ54_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0165615.1"
                     /translation="AGVVISSLLSAWTSALLLLDENTMDIVRFWSAGSVAGRSLSIFW
                     DISPFLIGGAVVGLFLGHQLNVMSMGEETARSLGMNTGRTRVISSVLVVIITGAAVSA
                     AGPIGFVGLATPHVVRSIVGPDYRWILPYSLVIGAMFLTAADIVGRVIARPGEIQVGI
                     VTAVLGAPFLIYLARQRTVAS"
BASE COUNT          816 a         1345 c         1374 g          676 t
ORIGIN      
        1 tcatctcgac cgtgtcgaaa cctcgctcat ggcgctcgcc cgccatgaac tcggcgagaa
       61 cgcccgcttc gtgggcggcg actcattcat tctctgggct tcgccggatc gggccagtca
      121 cgtgccactg ggacgctacg aactaccacg ccgtaatcct gatgcttata tctttcgcct
      181 gggtcatcca ctcgcagact tcctgattgc ccaagcgaag aaccgatcat tgtcgaccgc
      241 ccacatcgtc ttcgactaca cggcgtacga acgaaagata tcgtcaatcg agagattggt
      301 cggcacgtcg ggctggatgt ccgtttccct gctgacgctt gatggaagcg aggttacgga
      361 ggaccacgtc gccgttgctg cgatccggga tgacttgcag ccggttcccg ccgaggacgc
      421 gatgcgcttc tttactctac cgggatatat cggagaggat gcgccatccc cttcatcttt
      481 catctccgat ctgcgcatcc aggccgagtc cgtgacgaac gacattgtcg cgggggctgt
      541 cgagcgcaat gccgaatacc tcgaagtgga atccgagaag ctcgacaagt gggccgacga
      601 tctccgtcag tcgcttcgtg gtgaactcag gtctctcgaa gaactcattg gtgagactcg
      661 tcgccagatc aagatcaccg ggctcgcgag cgaacggaac gccgttcgcc ggcgaatcaa
      721 ccagctcgag gcagaactgg acgagaagcg gaatgaactc agccgtcgct cgcaggaaat
      781 tacccggcgg cgggacgatt tctatgacga tgtcgaccgc cgcttgcgcc aggaaagcac
      841 gattcacccc ctcttcacga tccgctggag cgtgatctag atgggccctt acacggcgat
      901 ctggagtggt tcgctggtga ttcgaatgtc ggttgcgaac cgatcgtccg ttggcgcggt
      961 gccgagatgc ggactcacgt ggcgctccgg catgatcatc accgcgccgg tgatcggatc
     1021 ggccgaaacc gccgcctcga tctcgaagac atcgcgcagg aggtcggtcg tcagcacctc
     1081 gtccggcgtg cccgtcgcga caacggcgcc atccttcatc gccacgatca ggtccgcgta
     1141 gcgggcggcc atgttcaggt cgtggaggat catcaggacg ctgtagccct tctcccggtt
     1201 gagctggcgc gccatgtcga gcaggtcgat ctgggccgcc atgtcgagga aggtcgtggg
     1261 ctcgtcgagg agcaacaccg gcgtgtcctg ggcgagcgcc atcccgaacc aggcgcgctg
     1321 acgctggccg ccagagaggg tgtcgacttc gcgatggcgg aattcgacga gattgcagcg
     1381 gtcgatcgcc tcgtcgatga tcccctcgtc ctcacgactc cactgcctga agatcgactg
     1441 gtgcggaacg cgccccgaag cgacaaggtc ttccaccgag aagccggacg gcggcaccgg
     1501 tccctgcgtg agcagcgaca tccgcttcgc gagcgccttg tgcgagccgg atccgaccgc
     1561 cttgccatcg agccgggcgt gaccgcccat cggcttcagc agccgggcgc aggaacggaa
     1621 gagcgtcgac ttgccacagg cgttcggtcc gacgatcgcg atcatcttgc cggccggaag
     1681 ctcaagcgag agatcctcga tgatcacgtt cttcccgtag ccggtcttca gacccgcaac
     1741 cgagaactca cccgccgtcg ccgccggctt cgaccgctcg tcgaatgcct gcgatccgct
     1801 gctgcgtggc atgcagaaga cgcaatcgcg cgccgcgtgc gggtagatgt cgcactcgac
     1861 gccatagagg cgcgcgagga ggtccggctg cagcacctcg tcgggggtgc cggaacgaag
     1921 gatctcgcca tcgcgcatcg cgacgatgcg gtggctgacg cgcgcggcct cgttgatgtc
     1981 gtggaggacc atcacgatcg tcttgccctc ccgctcgttg aggtgctgga cgagatcgat
     2041 gatctcgagc tggtgcgaga cgtcgagata ggtcgtcggc tcgtcgagga gcataatcgg
     2101 cgtgtcctgg gcgacggcca tcgccatcca cgcccgctgc cgctgcccac ccgagagttg
     2161 atcgaccgca cgtttgcgga gcgactccat tccggtcagc gcgagcgcgc gatcgatcgc
     2221 ctcttcatcc ttctgggagg gaggctggaa gaacgactgg tgcggatagc gtccgcgccg
     2281 gacgagatcc tcgaccatga tgccctgcgg cgtcagcgcc tgctgcgact ggagaccgag
     2341 ccgcttcgcg acctcgcgcg tcgggatcga gtggatgagt tgcccatcga gatgcacctg
     2401 accggaatcg ggtcgcatca gccgcgcgag cccccgcagc aaggtcgatt tcccgcagcc
     2461 gttgggaccg atgatcgtcg tgatctcgcc atcctggata tccaggctga gatccttgac
     2521 gatcgtctgc tcgccgtagg aaagggtcag gttctcggaa atcaggcgac tcatgggcgt
     2581 tacatccgga cgttggaacg atagagcagg aagaggaagt acggggcccc gacggcggag
     2641 gtgacgacgc cgaccggaag cgcgaccggc aggaagtgct gggcgacggt gtccgcggcg
     2701 aggaggaaga aggcgccgag cagcgccgtc agcacgagca cgctgctgga gagcgcgcca
     2761 ccaagcatcc gcgcgatgtg cggcaccatc agcgcgacga acccgatcgg tccggcaacg
     2821 gagacggtca ccgcggcgag accgcagcca acgagcatca ggccgaggcg cgtccgctcg
     2881 atcgggattc ccagtccgcg cgcggcgtca tcgccaagct ggagcgcgcg caggtgccac
     2941 atcatgccga caccgatcgg caggcagacg atgacgaagc ccagcagcgt cttgacgtcg
     3001 gtccagttcg atccgtagat cgaccccatc gtccagacga tcgcaggtcg cacctggtcg
     3061 atcgggaagc ggaccgtgat gaacgtcgtc ccggcgctga tcgcggcacc aacaccgata
     3121 ccgacgagaa tcaggcgatt cgggttgatc ccccgcttcc atgaaagcag gtagaccagc
     3181 atcgccgcgc tgacggcgcc gatgaacgcc cccagcggca accactggga attgaacccg
     3241 aagacgatcc agaagacggc gacgagcgtc gccccggtat cgatcccgat gatgtccggc
     3301 gagacgagcg gattgcggac aagtccctgg aagatcgcgc cggccatcgc cagcgcggcg
     3361 ccgatcagca tcgcgcagat caaccgcggc aagcgcagcg tgcggacgat gaactcctgg
     3421 tcgtcattcc cgtgcccgaa gacggacttc acgacatcga cgaaggggat ccggtagctg
     3481 ccgagcgtca tcgcgaagac cgcgagcacg gcaagcagcc cgaagacgaa caacgacatg
     3541 ccgaggaccc gaacgttgaa gcggacctgg agccacggcg tgcgcaccgt gacatgaccc
     3601 ggcttgtgga agccgggggc cgtctggttc gcgcccgggg tcgcgagatc gagggttgcc
     3661 atgcctagct cgccaccgtt cgctgccgcg cgagatagat caggaagggt gcgccaagca
     3721 cggccgtcac gatcccgacc tggatctcgc ccggtcgggc gatcacgcgt ccaacgatgt
     3781 cggcggcggt gaggaacatc gcgccgatga cgagcgagta gggaaggatc cagcggtaat
     3841 cgggtccgac gatcgagcgg acgacatgcg gcgtcgcgag accgacgaag ccgatcgggc
     3901 cggcggcgga gaccgcggcg ccggtgatga tcacgacgag caccgaggag atgacgcgcg
     3961 tccggcccgt gttcatgccg agactccgcg cggtctcctc gcccatgctc atcacgttga
     4021 gctggtgtcc aaggaagagg ccgaccaccg cgccaccgat caggaacggc gagatgtccc
     4081 agaagatcga gagcgagcga ccggcgaccg accccgccga ccagaagcgc acgatgtcca
     4141 tcgtgttttc atcgagcagg agcagcgcac tcgtccaggc actgaggagc gaggagatga
     4201 cgacaccggc g
//
LOCUS       DASTMD010000761         3818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_158045, whole genome shotgun sequence.
ACCESSION   DASTMD010000761 DASTMD010000000
VERSION     DASTMD010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158045"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFQ54_11285"
     CDS             <1..389
                     /locus_tag="VFQ54_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165616.1"
                     /translation="WLTVARIVRAQTLSLKEKEFIEAARGLGATHSQIMFKHLLPNTA
                     ATVVVATTLGIPSAILYEAGLSFLGLGVIPPTSSWGLMISEAIPNMRSHSYMLIFPAI
                     ALSLTVLAFNFLGDGLRDALDPWMKR"
     gene            1073..1516
                     /locus_tag="VFQ54_11290"
     CDS             1073..1516
                     /locus_tag="VFQ54_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEU0165617.1"
                     /translation="MVAEGVRGTYRNTTQRAVILAEVESAEGHLTAGEIFERVRRRYP
                     TIAYGTVYRTLHLLAEHELIQELTFADQASRFDKRTERHDHVLCARCGAIADVEVPVA
                     LLAEHVAQEQSGFMVTSHHTMFNGLCPSCQLAIEREAAEERASVV"
     gene            1669..2469
                     /gene="sufC"
                     /locus_tag="VFQ54_11295"
     CDS             1669..2469
                     /gene="sufC"
                     /locus_tag="VFQ54_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU0165618.1"
                     /translation="MSENTNAPMFEIENLHASIEDTEILKGVNLTINKGEIHAIMGPN
                     GSGKSTLANVLAGNPVYEVTEGSARFYGEDILELEPDERAQLGIFLAFQYPTVIPGVS
                     MANFLRMAVTNVRKARAEKAGDAGGGITPREFRRLMREKMQVLRMDESFATRYLNEGF
                     SGGEKKRAEILQLALLEPSLAILDETDSGLDIDALRVVSEGVNALAGPEMGFVLITHY
                     QRLLNYIKPHFVHIMIEGRVVQSGGPELALELEAKGYEKLVAEFATPA"
     gene            2539..>3818
                     /gene="sufB"
                     /locus_tag="VFQ54_11300"
     CDS             2539..>3818
                     /gene="sufB"
                     /locus_tag="VFQ54_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU0165619.1"
                     /translation="MVAKAQVDIGDYKYGFHDEEDYVFKSKKGLTRKVVEEISYMKNE
                     PDWMRDFRLKSFEHFEKRPMPNWGSPAIKDINFDDIYYYIKPAAEQGKTWDEVPAYIK
                     DTFEKLGIPEAERSFLAGVGAQYESEVIYHSLREDLEKQGVVFLDMDGAVRDYPELVK
                     QYFGTIIPYQDNKFAALNSAVWSGGSFVYVPENVRIEVPLQAYFRINAENMGQFERTL
                     IICAPGSYVHYVEGCTAPTYSTDSLHSAVVEIVVQEGARTRYTTIQNWSKNVYNLVTK
                     RAAAYKDATMEWVDGNLGSKVTMKYPAVWMMEPGARGEVLSVAFAGDGQHQDAGAKMV
                     HAAPHTSSQITSKSISKGTGRSSYRGLVKVHKGAEHVRSNVVCDALLLDETSKTDTYP
                     HMEIEEERVSIGHEATVSKVNDEQIFYLQSRGLN"
BASE COUNT          751 a         1214 c         1172 g          681 t
ORIGIN      
        1 gctggctgac cgtcgcccgc atcgtgcgcg cgcagacgct ttcattgaaa gagaaggagt
       61 tcatcgaggc ggcgcgaggt ctcggtgcga cgcacagcca gatcatgttc aagcatctgc
      121 tgccgaacac cgcggcgacc gtggtcgtgg caacgacgct cgggattccg agcgcgattc
      181 tctatgaagc cggtctgagc ttcctcggtc tcggtgtgat cccgccgacc tcgagctggg
      241 gcctgatgat cagcgaggcg atcccgaata tgcggtcgca ctcctacatg ctgatcttcc
      301 cggcgatcgc gctctccctg accgtgctgg ccttcaactt ccttggcgat ggtctccgcg
      361 acgcgctcga cccgtggatg aagcgctagc gatttccggc gcgatttctc gctcgagcct
      421 catcgcggaa cacgaccctc aagcccgtcg agaaaccgtg ccgatcccac aagccgtgcg
      481 caacgcctcc ccagccacgt cttcccgagc ttgtcgaggg atcgcgtggg tgccacgtgt
      541 ttccctcgtg acgccgttcc cgtcgtctgt tcgccgccga tgcacgatcg cgcacgggcg
      601 tcgacccgaa cgcgccttga aaggggggga catagaagtc cccccgtact cacgaccgct
      661 gcgtgaacgg ccttgatgac acgatccggc gccgttcaag acgaccgggg tttgccgggc
      721 gggagtctat ctctcgccgc gagcggagcc cccacatgat gggatctatc gggctgtgcc
      781 cgaccggtgc gctgccgggt cagaccagca gacccgcgac ggaatggtcg gttcgtggcg
      841 gcaagaggcg cgcccgttgt catatccgat gcattagatc ggatatggtg ctatctttgt
      901 ggtcgacggt gactgaccgc cgggtccggc ccgtcaattt cgacgaccgt gctatacttg
      961 gctcctgtaa acgagactca ttcgcatcag cgaagcggag caacatccga ggttcgcgcg
     1021 gatcgcagtc caacgtttta cggagtgttc gccaggagaa ggaacgacgc ccatggttgc
     1081 cgaaggagtg cgcggtacct accgcaacac aacccaacgc gcggtgatcc tcgccgaggt
     1141 ggagtccgcc gaagggcatc tcaccgctgg cgagatcttc gaacgggtgc gtcgacgcta
     1201 tccgacgatc gcgtatggaa cggtctatcg aacgctgcat ctgctcgccg agcatgagct
     1261 gatccaggag ttgaccttcg ccgatcaggc gagccggttc gacaagcgta ccgagcggca
     1321 cgatcatgtg ctgtgcgcgc gctgtggcgc catcgccgat gtcgaagtgc cggtcgcgct
     1381 ccttgccgag catgtggcgc aggaacagag cggcttcatg gtcacgagcc atcacacgat
     1441 gttcaacggg ctctgcccat cctgccaact cgccatcgag cgtgaggcag cggaagagcg
     1501 agcctccgtc gtctaggtat cgcgggttcg gacctggcgg tgacacacgc gctgccaggt
     1561 ttttcattgg tcggagcggt gatcccaccg catcgtgcag gcatccgcat cacagcgacg
     1621 cgaagttgcg gatgagttca attcccaggg agggttccta cacacgccat gtctgagaac
     1681 accaacgcac ccatgttcga aatcgagaac ctgcacgctt ccatcgagga cacagagatc
     1741 ctcaagggcg tgaacctgac gatcaacaag ggcgagatcc acgcgatcat gggccccaac
     1801 ggctccggca agagcacgct ggcgaacgtc ctcgccggca atccggtcta cgaggtcact
     1861 gaaggatctg ctcgcttcta cggcgaggac atcctcgaac tcgagccgga tgagcgcgct
     1921 cagctcggca tcttccttgc cttccagtat ccgaccgtca tccccggcgt ctcaatggcg
     1981 aacttcctcc ggatggccgt caccaacgtg cgcaaggcga gggcggagaa ggccggcgac
     2041 gcgggtggcg ggatcacgcc gcgcgagttc cgccggctga tgcgcgagaa gatgcaggtc
     2101 ctcaggatgg acgagtcgtt cgcgacccgc tacctgaatg aaggcttctc cggcggcgag
     2161 aagaagcgcg ccgagatcct tcagctcgcc ctgctcgagc cgtcgctcgc gatcctcgac
     2221 gagaccgatt ccgggctcga catcgacgcg ctccgcgtcg tctccgaggg tgtgaacgcg
     2281 ctcgccggcc ccgagatggg gttcgtcctg atcacgcatt atcagcggct gctgaattac
     2341 atcaagccgc acttcgtcca catcatgatc gagggacgag tcgtgcagag cggtggtccg
     2401 gagctggctc tcgaactcga ggccaaggga tatgagaagc tcgtcgccga gttcgcgaca
     2461 cccgcctgat catcgtttct cgcctgaacg ccgacgcagc agtcttccgc ccggccacat
     2521 caacaggagc acaccactat ggttgccaag gcacaagtcg atatcggcga ttacaagtac
     2581 ggcttccacg acgaagaaga ctacgtgttc aagtccaaga agggcttgac ccggaaggtc
     2641 gtcgaagaga tctcgtacat gaagaacgag ccggactgga tgcgggactt ccgcctcaag
     2701 tcgttcgagc acttcgagaa gcggccgatg ccgaactggg gcagccccgc gatcaaggac
     2761 atcaacttcg acgacatcta ctactacatc aagcccgccg cggagcaggg caagacctgg
     2821 gatgaggttc cggcctacat caaggacacc ttcgagaagc tcgggatccc cgaggctgag
     2881 cgaagcttcc tcgccggtgt cggcgcccag tacgaatccg aggtcatcta ccactcgttg
     2941 cgcgaggatc tcgagaagca gggcgtggtc ttcctcgaca tggatggcgc ggtccgcgac
     3001 taccccgagc tggtgaagca gtacttcggg acgatcatcc cgtatcagga caacaagttc
     3061 gcggcgctca actctgcggt ctggtccggt gggtcgttcg tctacgttcc ggagaatgtc
     3121 cggatcgagg ttccgctgca ggcttacttc cgcatcaatg cggagaacat gggccagttc
     3181 gagcgcacgc tgatcatctg cgcgccgggc agctacgtgc actacgtcga gggctgcacg
     3241 gctccgacct acagcacgga cagcctccac tccgcggtcg tcgagatcgt ggttcaggaa
     3301 ggcgcccgca cccgctacac gacgatccag aactggtcga agaacgtcta caacctcgtc
     3361 accaagcggg ccgcggccta caaggacgcg acgatggagt gggtcgatgg caacctcggc
     3421 tccaaggtga cgatgaagta cccggcggtc tggatgatgg agccgggcgc tcgcggtgag
     3481 gtcctctcgg tcgccttcgc cggtgacggc cagcatcagg atgccggcgc gaagatggtt
     3541 cacgccgctc cgcacacctc ctcccagatc acctcgaagt cgatctcgaa gggcaccggt
     3601 cgctccagct atcgcggtct ggtcaaggtc cacaagggcg ccgagcacgt gcgcagcaac
     3661 gttgtctgtg acgcgctcct gctcgatgag accagcaaga ccgacaccta cccgcacatg
     3721 gagatcgaag aggaacgcgt ctctatcggc cacgaggcaa ccgtctcgaa ggtcaacgac
     3781 gagcagatct tctatctgca gagccgcggc ctcaacga
//
LOCUS       DASTMD010000762         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2392396, whole genome shotgun sequence.
ACCESSION   DASTMD010000762 DASTMD010000000
VERSION     DASTMD010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392396"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFQ54_11305"
     CDS             <1..386
                     /locus_tag="VFQ54_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165620.1"
                     /translation="VFVLCGVSLVMFAAPPTKWFVVVEETNGDWRPTIVAAAMLPLYV
                     IIMSLPGLRTFFGLNLLESWLYVLIAVVVPGWMIALRYVWKNGIFDRFIDSPVPSKLI
                     DYLERDRTRRPPEPTERVVAVTPER"
     gene            462..1400
                     /locus_tag="VFQ54_11310"
     CDS             462..1400
                     /locus_tag="VFQ54_11310"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU0165621.1"
                     /translation="MAADEFADGLAAALFPDDLVSPEWLAAHLDKPGLQVVDMRGYVK
                     TEDLGGGRQKGVYLGARDEYEAAHIPGATYVDWTSDIVDPDAEVKAQIAPPMVFARRM
                     ESVGVGDDTSVVVVDHAGGHLATRLWWALRYYGHENVAILDGGYQVWQELNLPLTSEP
                     TTPPEVAVAFTPRPVRKLISDRDDVLAAVLSGDRQVLDARITAQYSGEVQRGTRGGHI
                     PGAVNLPAQSLVRRDGRWMTREEIRLEVHAAGIDLDQPVTAYCNGGVTATQLLFGLHR
                     AGATDISNYDGSWNEWGERLELPVEGNRDLFANEAE"
     gene            complement(1479..>2057)
                     /locus_tag="VFQ54_11315"
     CDS             complement(1479..>2057)
                     /locus_tag="VFQ54_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165622.1"
                     /translation="STSIPVTTVPTGTVAAAGDVILTLSTGDGGSVPPGTKVCIADRC
                     QTVGAEISGAAVSDATLRFATLAPGTYPLAITNAAPYQDATGNVTIADGQTTAVDIVL
                     ERAAATTPTEPGAATPIVTRAATIAPTREPGGGAAVSPGQKAPSGNGQVVTSLPNTGS
                     GAQSPTASVALLVLAMAAILGACVLNWRRRRI"
BASE COUNT          334 a          639 c          709 g          375 t
ORIGIN      
        1 atgtcttcgt gctctgtgga gtcagcctgg tgatgttcgc cgcgccgccg acgaagtggt
       61 tcgtcgtggt cgaggaaacc aacggggatt ggcgaccgac gatcgtcgcg gccgcaatgc
      121 tgccgctcta cgtcatcatc atgtcgctgc cggggctccg caccttcttc gggctcaacc
      181 tcctggagag ctggctctac gtcctcattg cggtggtcgt gccgggctgg atgatcgcgc
      241 tgcggtatgt gtggaagaac ggcatcttcg atcggttcat cgactcgccg gtgccgtcga
      301 agctgatcga ttacctcgaa cgggatcgca cgcgacgacc gcctgaaccg actgaacggg
      361 tagtagcggt cacgcccgag cgttagtaca tttgagcggt cataccgggc ccgggtcccg
      421 cccgggcatc gctctcgtat gaagcaacga aaggaacggg catggcagca gacgagttcg
      481 ccgatggcct ggccgcggca ctcttcccgg acgatctcgt ttcgccggaa tggcttgccg
      541 ctcatctcga caagccgggt cttcaggtcg tggacatgcg cggctatgtg aagaccgagg
      601 atctcggcgg tgggcgccag aagggcgtct atcttggcgc gcgcgacgag tacgaggcgg
      661 cgcacattcc tggtgcgacc tacgtcgact ggacatcgga tatcgtcgac cccgacgccg
      721 aggtgaaagc ccagatcgcg ccgccgatgg tcttcgcgcg acggatggaa tcggttggcg
      781 tcggtgacga cacgagcgtc gttgtggtcg accatgcggg cggccacctt gcgacccgcc
      841 tctggtgggc gctgcggtat tacggtcatg agaacgttgc gatcctcgac ggtggctatc
      901 aggtctggca ggaactgaac ctgccgctca cgagcgaacc gacaacgcca cccgaggttg
      961 cggttgcgtt cactccgcga ccggtgcgga agctgatcag cgaccgtgac gacgtgctcg
     1021 cggccgtgct gtcgggcgat cgacaggtgc tcgatgcccg catcaccgcc cagtactccg
     1081 gcgaggtcca gcggggcacg cggggcggcc acattcccgg agccgtcaat ctgccggcgc
     1141 aatcgctggt gcggcgtgac ggtcgctgga tgacccgtga ggagatccgg ctggaggtac
     1201 acgctgccgg catcgatctc gatcagccgg tgacggcgta ctgcaacggg ggcgtcacgg
     1261 cgacgcagct cctcttcggg ttgcatcgtg ccggggcgac ggatatctcc aactacgatg
     1321 gctcgtggaa cgaatggggc gaacggctgg aactccccgt cgagggaaat cgcgatctct
     1381 ttgcgaacga ggcggagtag atcgatcgtg cgatcgggaa tgagagaggc cgtccgggag
     1441 ttcccggacg gcctctctct gtacggtcga ccgtgtgact agattcgccg gcgtcgccag
     1501 ttcaggacgc acgcaccgag gatggccgcc atcgccagca cgaggagggc cacactggcg
     1561 gtcggcgact gcgctcccga tcccgtgttc ggcaaggagg tgacgacctg cccattcccc
     1621 gacggtgcct tctggcccgg tgaaaccgcg gctccgccac cgggttcccg agtcggtgca
     1681 atcgttgcgg ctcgcgtgac gatcggcgtc gcggcgccgg gttccgttgg cgtggtcgcc
     1741 gccgcccgct ccaacacgat gtcgaccgcc gtcgtctgac cgtctgcgat cgtcacgtta
     1801 cccgtggcgt cctggtatgg ggcggcattg gtgatcgcga gcggatacgt gcccggagcg
     1861 agggtggcaa agcgcagcgt tgcatcggaa acggcagcgc cgctgatttc cgctccaacg
     1921 gtctggcagc gatcggcaat gcagaccttc gtgccaggtg gaacgcttcc tccatcgccg
     1981 gtcgagagcg tcaggatgac gtcgcccgcc gccgcgaccg ttcccgttgg gaccgtggtg
     2041 acggggatcg atgtcga
//
LOCUS       DASTMD010000763         1742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1501014, whole genome shotgun sequence.
ACCESSION   DASTMD010000763 DASTMD010000000
VERSION     DASTMD010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501014"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..734)
                     /locus_tag="VFQ54_11320"
     CDS             complement(123..734)
                     /locus_tag="VFQ54_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEU0165623.1"
                     /translation="MTDTLKTPALGSDKTPTFTLDFLSWTPWIEKATETDLTPEQRDR
                     VTAVVSDHPNSAYQAVLANDFPALQTRAKVHHHIFTSEEPEPTAWRELGASTTSRING
                     CVFCLSVHARIYAQKSGKRDVALTFLEDGLAAELPETERAVVDLAAKVTLDPESLSQA
                     DFAALRDLGFSDLAILDVINYAAFFANANRLMLTLGEPVPAEK"
     gene            complement(731..1252)
                     /locus_tag="VFQ54_11325"
     CDS             complement(731..1252)
                     /locus_tag="VFQ54_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain protein"
                     /protein_id="HEU0165624.1"
                     /translation="MSASTTRTTDVVYSILGVGEDSPIAALRNDRPNQIDELQAYYLS
                     LFAPEAASAEAFPLKKRYLVAIRVASHTGSTDIVAWYEHLAREAGVSDEKIAATKDIA
                     TPATDDSVLGAAIRHADLLTTHPVDAEASDLQALKDAGLTPAGILSLSQVIAFVSYQL
                     RFIAGLRAFGGQA"
     regulatory      complement(1378..1483)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT          301 a          520 c          597 g          324 t
ORIGIN      
        1 ttgacctggg gcgaccacgt gggttgccgg gacgagaacg aaacgcgcac cgggagcgcc
       61 gactgcgacc cgttcaccgc cccgcccggg ttatgcgaca gcgttggccc tcacccgttc
      121 ccttatttct cggcaggaac cggttcgccg agcgtgagca tcagacgatt ggcgttcgcg
      181 aagaaggcgg cgtagttgat cacgtcgaga atcgcgagat cgctgaagcc aaggtcgcgc
      241 agcgccgcga agtccgcctg cgagagcgac tccggatcga gcgtcacctt cgcggcgaga
      301 tcgacgacgg cgcgttccgt ctcggggagt tcggcggcga ggccatcctc gaggaaggtc
      361 agggcaacgt cacgcttgcc cgacttctgg gcgtagatgc gggcgtgaac cgagaggcag
      421 aagacgcaac cgttgatgcg ggacgtcgtc gaggcgccaa gttcgcgcca ggctgtcggt
      481 tccggctcct ctgaggtgaa gatgtggtga tgcacctttg cccgcgtctg gagcgccggg
      541 aaatcgttgg caaggactgc ctggtaggcg gagttcggat gatcgctgac gaccgccgtc
      601 acgcggtcgc gctgttccgg agtcaggtcg gtctccgtcg ccttctcgat ccacggcgtc
      661 cagctcagga agtcgagcgt gaacgtcggc gtcttgtcgc tgccgagggc gggagtcttg
      721 agggtgtcgg tcatgcctgg cctccgaagg cgcgcagccc ggcgatgaag cggagctggt
      781 aggaaacgaa tgcgatcacc tgggaaagcg agaggatacc cgccggcgtc agtccggcat
      841 ccttcagcgc ctgcaggtcc gaggcttcgg catcgacggg gtgggtcgtc aggagatcgg
      901 cgtggcgaat cgccgccccg aggacggagt cgtcggtcgc cggcgttgcg atgtccttcg
      961 tcgccgcgat cttctcgtcg ctgacaccgg cttcccgcgc gagatgctcg taccaggcga
     1021 cgatgtcggt gctcccggtg tgggaagcaa cgcgaatcgc gacgaggtag cgtttcttca
     1081 gggggaacgc ttcggccgag gcggcttccg gggcgaagag tgacaggtag taggcctgca
     1141 actcgtcgat ctggttcggg cgatcgttgc gcagggcggc gatcggcgaa tcctcgccga
     1201 ctccgagaat gctgtagacg acgtcggtcg tccgggtcgt ggatgcgctc attggcgtcc
     1261 tcctgagagg tgacgagtat cgtcaccgtg agacggattc gatctcccgg aaaccggaaa
     1321 cccccaactc cttgcggatg ggggtaaagc gcgatcgcga acgagcgaag ctcttgcccc
     1381 tcatcttcca ggatctcctg tcggacttag caccttcccg ggtctgaacc gggaggttgc
     1441 tggggcttca tcgggccgat ccctctgccc ctctggatga ggtgatgtgg ttgttgccgg
     1501 tatcgggaga taccttacga aagccacttt agataagcga gtattgcgtg tcaagtcgtg
     1561 ttttgatgac cgccgcgata cgaaaagggt agatgacctc ccacgcggct cctcccaaga
     1621 cacgtcctcc gggcccgtcg aggggccagc cggcgtccac gtgcttcccg tccgacgcct
     1681 cacaggatac gtcttcccga gtagtcatga agcgcgcctc gccagcgcgg catgaaaaag
     1741 ct
//
LOCUS       DASTMD010000764         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2127121, whole genome shotgun sequence.
ACCESSION   DASTMD010000764 DASTMD010000000
VERSION     DASTMD010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2127121"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VFQ54_11330"
     CDS             <1..602
                     /locus_tag="VFQ54_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165625.1"
                     /translation="QRSGKPVDTALSFGNYALFALPSFWLGLIMIVIFSSKLGWLPTA
                     GMRNVRADYTGWRDRFDVLEHLILPVATLALVQIPIYFRITRASVLLQQREDYVTTFR
                     ATGIPESKIFRRYALRNALLPTVTVFGLSLGFVFTGAALVEIVFAWPGIGRVTLDAVF
                     RRDYPLLLGIYLVLAIAVSIAVLLTDLVYALLDPRIRLR"
     gene            602..1501
                     /locus_tag="VFQ54_11335"
     CDS             602..1501
                     /locus_tag="VFQ54_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165626.1"
                     /translation="MSQIQLAVEPTLELPSRRESRFRSFWRPFVASKMGPVGLVILIA
                     VALCAIFAGVIAPDGPFKLGGPRLVGPGEDGYLLGTDHLGRSMTAQLVYGARVSLSVG
                     VIAASISLIVGVFFGCIAGYFGGWIDILISRMTDMFLIIPAFFLIIVVVATLGSGITY
                     VMIVIGLTSWPTSARLMRAQAMSLRERTFIQGLKALGESSPRILFRHIIPNGIQPLIA
                     NASLDIASAILIEAGLSFLGLGDPNKATWGRMIFDGRATILTAWWTSVLPGIAIVITV
                     LGFYLLGDGISYVLNPRSRKVTA"
     gene            1498..>2124
                     /locus_tag="VFQ54_11340"
     CDS             1498..>2124
                     /locus_tag="VFQ54_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0165627.1"
                     /translation="MSTEAPLLDVRNLSITYRTDRGFVSAVQDVSFTVPRGGSIGLVG
                     ESGSGKSTIAMAVMRLLPLNARVTGEIILDGTDITKLSDRQLNRDWRWRKLSMVFQKS
                     LSALSPVHRIGAQMHDVLRQHSPSLSESERKEKIDRVLEAVNLPQRVIRSYPFELSGG
                     MMQRTMIALGLVCNPPFVIFDEATTALDVITQAQILSEVRRLKDEFDLT"
BASE COUNT          339 a          720 c          628 g          437 t
ORIGIN      
        1 cccaacgctc gggcaagccg gtggataccg ccctttcctt cgggaattac gcgctcttcg
       61 ccctgccgtc attctggctc ggcctgatca tgatcgtgat cttctcgtcc aaactcgggt
      121 ggcttccaac cgcgggcatg cgcaatgtgc gcgccgacta caccgggtgg cgcgatcgct
      181 tcgatgtgct cgaacacctg atccttcccg tcgccacgct ggccctggtg cagattccga
      241 tctacttccg gatcacgcgg gcgagcgtcc tgctgcaaca gcgggaggac tacgtcacga
      301 cgttccgcgc caccggcatc cccgaatcga agatcttccg tcgctacgcg ctccgcaacg
      361 cgctgttgcc gacggtcacc gtgttcggcc tgagtctcgg gttcgtcttc accggcgcgg
      421 cgctcgtcga gatcgtcttt gcctggcccg ggatcgggag ggtaacgctg gacgccgtct
      481 tcaggcggga ctatccgctc ctgctcggga tctacctggt cctggcgatc gcggtttcga
      541 tcgcggtgct cctgaccgat cttgtctatg cacttctcga cccacgaatc aggttgcgat
      601 aatgagtcaa atccagcttg ctgttgaacc aacattggaa ctcccgtcac gccgggagtc
      661 gcgattccgc tccttctggc gtccgttcgt ggccagcaag atgggcccgg tcggactcgt
      721 gatcctgatc gccgtggccc tctgcgcgat ctttgccggc gtcatcgcgc cggatggacc
      781 gttcaagctg ggcggccccc gcctcgtggg accgggtgag gacggctacc ttctgggtac
      841 cgaccatctc ggacggagca tgacggcgca actcgtctat ggcgcccgtg tctcgctcag
      901 cgtcggcgtc atcgccgcgt ccatctcgct gatcgtcggc gtcttcttcg gatgcatcgc
      961 cggctacttc ggcggatgga tcgacatcct gatcagccgc atgacggaca tgttcctgat
     1021 cattccggcc ttcttcctga tcatcgtcgt cgttgccaca ctcggcagcg gcatcaccta
     1081 cgtgatgatc gtgatcgggc tgacatcgtg gccgacgagc gcgaggttga tgcgcgccca
     1141 ggcgatgtcc ctgcgtgaac gcacgttcat ccaggggttg aaagcccttg gagagtcgag
     1201 cccgcgcatc ctgttccggc acatcatccc gaacggaatc cagccgctga tcgccaatgc
     1261 atcgctcgac atcgcctcgg cgatcctgat cgaggccggc ctctcgttcc tcggactcgg
     1321 cgatccgaac aaggcgacgt ggggccgcat gatcttcgat ggtcgcgcca cgatcctgac
     1381 cgcgtggtgg acctccgtcc tacccggcat cgcgatcgtg atcaccgttc tcggcttcta
     1441 cctgctgggc gacggcatct cctacgttct caacccgcgc agccgaaagg tgaccgcatg
     1501 agcacggaag ctcctcttct cgatgtgcgc aacctctcga tcacgtatcg caccgatcgc
     1561 ggcttcgtga gtgccgtgca ggacgtcagc ttcaccgtgc cccggggcgg ctcgatcggg
     1621 ctcgtcggcg agagtggctc cgggaagtcg acgatcgcga tggccgtgat gcgcctcctc
     1681 ccgctcaatg cccgggtgac cggcgagatc atcctcgacg gcaccgacat caccaagctg
     1741 agcgatcggc agttgaaccg cgactggcgc tggcgcaagc tgtcgatggt cttccagaag
     1801 tcactctcgg cgctcagccc cgtccatcgc atcggcgccc agatgcacga cgtgctccgg
     1861 cagcacagtc catcgctgag cgaatccgaa cgcaaggaaa agatcgatcg cgttctcgag
     1921 gcagtcaatc tgccacagcg cgtgattcgc tcctatccct tcgagctctc gggcggcatg
     1981 atgcagcgca cgatgatcgc actcggcctc gtctgtaatc cgcccttcgt catcttcgac
     2041 gaggcgacga cggccctgga tgtgatcacg caggcgcaga tcctctccga ggtccggcgc
     2101 ctgaaggacg agttcgacct gacg
//
LOCUS       DASTMD010000765         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3197780, whole genome shotgun sequence.
ACCESSION   DASTMD010000765 DASTMD010000000
VERSION     DASTMD010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3197780"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /gene="mreC"
                     /locus_tag="VFQ54_11345"
     CDS             <1..929
                     /gene="mreC"
                     /locus_tag="VFQ54_11345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU0165628.1"
                     /translation="RVTRRIQFASVRSNPACVPFIDARPQEHDDLASVRIPVLMKTVP
                     LRQTLTLVLIFVVVSASMIVLDRQNALTPLRDGLSEITDPISSAFMSASDGFGGQSNL
                     QQQLATVTAQRDQLNAENSQLKNVQQENAQLRQQAKIQQANPNWDIESATVIGTDPTG
                     TQKFVKINKGSADGIQKGMAVVSPYYYVGQVSEVEQHSATVMLIIDISMKVGAKLEDS
                     DADGTVVGEWQTGKQAMVMQHIDKNVAPKSGEKIVTSDLTRNIPAGIIIGVVDGTPVR
                     DAQTDQLQVDVAPAANFDNLDMVVVIKSYDPN"
     gene            916..2340
                     /locus_tag="VFQ54_11350"
     CDS             916..2340
                     /locus_tag="VFQ54_11350"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU0165629.1"
                     /translation="MTRTDRARFDPRLTAALTASKLTAFGIRRLGRGGGTSAPGLVAE
                     RIDPHLLTKITSRLDAGSIVVAGTNGKTTTSRMIADILTAAGYDVVHNRSGSNLVRGV
                     ASAFAAHASILGHPRGQIGVIEADEAAFPAIVKLSKPRYILLNNLFRDQLDRYGELDT
                     IARHWKTAFQSLDESTTIIVNGDDPTLVAITDDIRARRVTFGLDEINHRLDALPHAAD
                     AATCRVCGADLAYRALYLSHLGEWYCPNCGRERPKLDVTGSDIVLHGVDSLTLSATPR
                     DEAPVPLEIGVPGLYNAYNTLAAYAVTREAGVAPVAIQGALAKFRSAFGRIERLDYRN
                     RKLTMALVKNPVGFNEVLRMLTSGSDGLAVPTLIAINDRDADGRDVSWLWDVDFELLA
                     DGEGLLGTTGIRGSDMANRLKYAGVPLDRIKMLDNDLATGLDSFVDRLPEGSSGYILP
                     TYTAMLELRKTLADRGAVESFWQQ"
     gene            2377..3120
                     /locus_tag="VFQ54_11355"
     CDS             2377..3120
                     /locus_tag="VFQ54_11355"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEU0165630.1"
                     /translation="METSASPTLKIAWLYGTQMNIYGDRGNVLAIAQRARWRGVEPEI
                     VEVGLGEPIPDDVDVFFFGGGQDQEQVAVSYDLQGEKGERIKTAVEDGAALLSICGGY
                     QLLGHEYRPHDATPLPGISLFDVTSTAGAERFIGNVIVETESWGPLVGFENHSGLTFL
                     GESATPMGQVEVGRGNNGKDNTEGTRYKNALGCYMHGALLPKNPRLTDWLIEAGLTRR
                     YGSFTLQPIESETEERAHATAVERAHKTR"
BASE COUNT          674 a         1087 c          986 g          596 t
ORIGIN      
        1 gccgcgtcac acgccgcatc cagttcgcct ccgtgcgatc gaacccggcg tgtgtccctt
       61 tcatcgacgc aagaccccag gagcatgacg atcttgccag cgttcggatt cccgtgctga
      121 tgaaaacggt tcctcttcgc cagacattga ccctcgtgct gatcttcgtg gtcgtttccg
      181 cttcgatgat cgtgctcgac cggcagaacg ccctcacacc cctccgcgat gggctctccg
      241 agattaccga cccgatctcc agcgccttca tgagcgcctc ggacgggttc ggcggtcagt
      301 cgaacctcca gcagcaactc gcgaccgtga ccgcccagcg cgatcagctg aacgccgaga
      361 acagccagtt gaagaacgtg cagcaggaaa acgcccagct ccggcaacag gcaaaaatcc
      421 agcaggcgaa tccgaactgg gacatcgaat cggccaccgt catcggaacc gatcccaccg
      481 gcacccagaa attcgtcaag atcaacaagg gttctgcgga tggtatccag aaggggatgg
      541 ccgtcgtcag cccctactac tacgtgggcc aggtctcgga agtcgagcag cacagcgcga
      601 ccgtcatgct catcatcgac atcagcatga aggttggcgc caagctcgag gattccgacg
      661 ccgatggcac cgtggtcggc gaatggcaga ccggcaagca ggcgatggtg atgcagcaca
      721 tcgacaagaa tgtggcgccg aagagtggcg aaaagatcgt cacatccgac ctcacccgga
      781 acattccagc gggtatcatc atcggagtgg tcgatggcac cccggttcgc gacgcgcaga
      841 ccgatcaact gcaggtcgat gtcgcgccgg cggccaattt cgataatctc gacatggtgg
      901 tggtgatcaa gagttatgac ccgaactgat cgtgctcgct tcgatccccg gctgaccgcc
      961 gcgctcacgg cgtccaagct caccgcgttc ggcatccgtc gcctcggacg cggcggcggg
     1021 acctcggcgc cgggcctcgt tgccgagcgg atcgatcccc acctgctcac gaagatcacg
     1081 agtcgcctcg atgccggctc gatcgtcgtc gccggcacca acggcaagac gacgacctcc
     1141 cgcatgatcg ccgatatcct caccgcggcc ggatacgatg tcgtgcacaa tcgatccgga
     1201 tcgaacctcg tgcgcggcgt cgcgagcgcg ttcgcggcac acgccagcat cctcgggcat
     1261 ccgcggggcc agatcggcgt gatcgaagca gacgaggcgg cgttccccgc gatcgtcaag
     1321 ctcagcaagc ctcgctacat cctcctcaac aacctcttcc gcgaccaact cgatcgttat
     1381 ggtgaactgg acacgatcgc gcgtcactgg aaaacagcat tccagtcgct cgacgaatcg
     1441 accacgatca tcgtcaacgg tgacgatcca acgctcgtcg cgatcaccga cgacatccgc
     1501 gcgcgccggg tcaccttcgg tctcgacgag atcaatcacc gcctggatgc gcttccgcac
     1561 gcggccgatg ccgcgacctg tcgcgtctgc ggcgccgatc tcgcctaccg cgcgctctac
     1621 ctctcccatc tcggcgagtg gtactgcccg aactgcggtc gcgagcgccc gaagctcgac
     1681 gtgaccggaa gcgacatcgt cctccatggc gtcgattccc tcacgctgag cgcgacgccc
     1741 cgtgacgaag cacccgtccc tctcgagatc ggcgtgccgg gcctctacaa cgcctacaac
     1801 acgcttgcgg cctatgccgt gacccgtgag gcaggcgtcg cacctgtggc gatccagggc
     1861 gcccttgcga agttccgttc cgcgttcggt cgcatcgagc gactcgacta ccggaatcgg
     1921 aagctgacga tggcgctcgt caagaatccg gtcggattca acgaggtcct ccggatgctg
     1981 acgtccggct cggatgggct cgcggtgccg acgttgatcg cgatcaacga tcgcgatgcc
     2041 gacggacgcg acgtctcctg gctctgggat gtcgacttcg agctgcttgc cgatggcgag
     2101 gggcttctcg ggacgaccgg gatccgcggc agcgacatgg ccaaccgcct gaagtacgcc
     2161 ggcgtcccgc tggatcgcat caagatgctc gacaacgacc tcgcgaccgg gctcgactcc
     2221 ttcgtcgatc ggttgccgga gggatcgagc ggatacattc tcccgaccta tacggcgatg
     2281 ctcgaactcc gcaagacgct cgccgatcgc ggggccgtcg aatcattctg gcaacaatag
     2341 tccgtccgct cagtcgcgtc cgtcacgagg aatgccatgg agacgtcagc ttcccccacg
     2401 ctgaagattg cctggctcta cggaacgcag atgaacatct atggcgatcg cggcaacgtg
     2461 ctggcgatcg cccagcgcgc gcgctggcgc ggcgtcgagc cggagatcgt tgaagtcggt
     2521 ctcggtgagc ccattcccga cgatgtcgac gtcttctttt tcggcggcgg gcaggatcag
     2581 gagcaggttg ccgtctcgta cgacctccag ggggagaagg gcgagcggat caagacggcc
     2641 gtggaggacg gcgccgcctt actttcgata tgcggcggat atcaattact cgggcatgaa
     2701 tatcgtccac acgacgcaac gcctcttccc ggaatcagtc tctttgatgt aacatccacc
     2761 gccggtgccg agcggtttat cggcaatgtc atcgtcgaga ccgaaagttg gggcccgctc
     2821 gttggattcg agaaccacag cggcctgacc ttcctcggcg aaagcgcgac gccgatgggt
     2881 caggtcgagg tcggccgcgg caacaacggc aaggacaata ccgaaggcac acggtacaag
     2941 aatgcactcg ggtgctatat gcacggcgcc ctgttaccga agaatccacg cctgacggac
     3001 tggttgatcg aagccggact cacccggcgc tacggttcct tcacgcttca accgatcgaa
     3061 agcgagaccg aggagcgtgc gcacgcgacc gcagtcgagc gagcacacaa aacacgctga
     3121 gattcggcgt cgaccgcgaa tccatattcc gttcggttcc tcaatcctgg gttcaataaa
     3181 gtgaaacggc gatccttgtt caatatggat gggggcgcta gacttacggc gcgtgaaatc
     3241 gcaatggtca tggcgcggtt ctatcgtatc atcgctctga agacgccatg acacagttcg
     3301 tcatcatcat cgatgcaaag gagggaatgt gtcttcaata gat
//
LOCUS       DASTMD010000766         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2928888, whole genome shotgun sequence.
ACCESSION   DASTMD010000766 DASTMD010000000
VERSION     DASTMD010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2928888"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VFQ54_11360"
     CDS             <1..987
                     /locus_tag="VFQ54_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165631.1"
                     /translation="PTSYAYWDYITGNQQISSGDEQNGRRFPVYIFVAQDGQWKLDGL
                     TWGCSGNCADAPDDILGGHFFTNEVNAPTSATATAEARIAPVDASTCTLPTTVSSDAP
                     ATPTDRGYVPIVRLDAARASYLTMAGEVTRAANNVVACAELNPDSLTGLETTRLSAES
                     ALTGGTPVLTDIQVSEAQSLSFWAHSLSLYVQPAGDAFGSPANATSPTVWPLSISGDP
                     VDRSSGWWTKALSSGAAMLPDGRIGVSLTYFTASDNAWQQQTDAAYEVVPFLIFSQEN
                     SSLKLDEVLPLCFGQCDAFWQGLSSGSTLVPASPAPVATPVAIDESRRAFWI"
BASE COUNT          327 a          510 c          483 g          298 t
ORIGIN      
        1 ccgacgagct acgcctattg ggactacatc accggcaacc agcagatttc atctggcgac
       61 gaacagaacg gtcggcgatt ccccgtctac atctttgtcg cacaagacgg gcagtggaaa
      121 ctcgatggtc tgacctgggg atgttccgga aactgcgctg acgcccccga cgacattctc
      181 ggaggccact ttttcacgaa cgaggtgaac gcaccgacgt ctgcgacggc aaccgccgag
      241 gctcggatcg cgccagtcga cgcaagcacc tgcactctgc caacgaccgt gtcgtcggac
      301 gccccggcca cgccgacgga ccgcggctac gtgccaatcg tccgcctcga tgcggcaagg
      361 gccagctatc tcacgatggc tggtgaggtc acgagggcgg cgaacaatgt cgtcgcctgt
      421 gccgaattga atcccgacag tctcacaggg ttggaaacaa cgcgactgag cgccgagtcg
      481 gcgctaactg gcggcactcc cgtgttgact gacatccagg tcagtgaggc gcagtcactc
      541 tccttctggg cacattcact ctcactctat gtgcaaccgg cgggcgacgc attcggatcc
      601 ccggcgaatg ctacctcgcc aacggtctgg ccactctcca ttagtgggga tcccgttgat
      661 cgatcgtcgg gctggtggac gaaagcgcta tccagcgggg ccgccatgct cccggatggc
      721 aggatcggcg tgtcgctcac ctacttcacg gcaagcgaca acgcatggca gcaacagacg
      781 gacgctgcgt acgaggtcgt gccgttcctg atcttctccc aggagaacag ctcgctcaag
      841 ttggacgagg tgctcccgct ctgcttcggc caatgcgatg ccttctggca agggctctcg
      901 agcggttcca ccctggttcc agcgtcaccc gctcccgttg cgacaccggt agcgatcgac
      961 gagagtcgtc gcgctttctg gatctgaccc ggcggccttc gcgatgatcg aagcgcgcct
     1021 catgcgaccg gacgcgcacg tctcatgatg cggatcgcaa acacggcgaa acctgaaacg
     1081 agcgtcgctg tggcaacgac cccgggtatg tagggcaagt ccatgtgtct tgccgcagac
     1141 tggctcgcgg cgagagaaat acgtaatcat ttcggacacg aatacgtaac gattgctgat
     1201 gcacgccccc ggcgttcgcc ctaaactcgg tacatggaac gcagatgacc gtgaaccgaa
     1261 aggagcgatc agatgggaca gacaatgctg tccaggcttc gaacgctggt gcgacgcttc
     1321 ctggctcttg attcgcaaaa gaccgcggag ggtgacgagg acgaaacacc gatcacgctc
     1381 ctccgaaaat ggatctgaca gcgaatcggc ccggtcccgg tgcctccagt ggccttacag
     1441 cgcatttcac atcgctcggc cgaggacact ccaggatatg cagggaaagg tttcgtgcct
     1501 tgccgtagac gggatcgcct ttggcgaccg ccggcgagag tcgcgtctcc ggttcatcca
     1561 gcgacggtgc tctgtgagtc gcgtcaacgc aacgatcata tcgggctctg cccgatgg
//
LOCUS       DASTMD010000767         6066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_607857, whole genome shotgun sequence.
ACCESSION   DASTMD010000767 DASTMD010000000
VERSION     DASTMD010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607857"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VFQ54_11365"
     CDS             <1..1290
                     /locus_tag="VFQ54_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU0165632.1"
                     /translation="RKPGVYLMHDQFGNIIYVGKAKSLRERVSSYYSQPLGYTRKMDG
                     LLESMVRIETIVVGSEIDALLLEAQLIRRYQPRYNTAMRSFEHYPYIRVDVSNAWPRI
                     SLAKTRKDDGAEYFGPYRSSSGAKRTVDVINAALPLRTCTRSFKNARSYGNPCILLDM
                     GKCLGPCVGRADRDEYQRIVRDVVQLLDGRDQALMERLWAELEIAAEKLDFEKAGKLR
                     RDLRSVLGLVENQQRLREAEQLHNLVLVLPSPDVAFRELVLIFGGRIWARFRMKREAS
                     REGAFVPAIVADDTVLSIDSFRTGSLRKVAEDRVVYGDGREIDDLASRLARAAERHAE
                     RGDDPVDNYLVDENNIINRWLMQHAGHPALLPLADDMDRGAWHHLLITALALTDDDLQ
                     FRERVRIDGEGEADEGGDVIETIAPTAGQGASAAVGI"
     gene            1450..2088
                     /locus_tag="VFQ54_11370"
     CDS             1450..2088
                     /locus_tag="VFQ54_11370"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU0165633.1"
                     /translation="MTVSDQGSESAEKLSVIASEVKVCTLCELSRTRTNAVPGEGDAN
                     ARVMLIGEGPGWHEDQEGRPFIGNAGKFLTELLEKAGLRREDVFITNVVKCRPPSNRD
                     PLPDEIHACSLYLERQIATIDPDVIVTLGRFSMAKYFPGERISRIHGQAKKLGNRLVV
                     PMYHPAAALHQGNLRGEIEEDFAKLPKLLAERERERDAEVEREAPPRQISLF"
     gene            2117..3787
                     /locus_tag="VFQ54_11375"
     CDS             2117..3787
                     /locus_tag="VFQ54_11375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU0165634.1"
                     /translation="MGEPTVKLIFLGGIAEVGKNLFLLETDDDIIVVDCGLGFPEEDQ
                     LGIDLVLPDISYLRERREKIRGIFITHGHEDHIGGIAYLWPDLGAPVYATRLTAGLIQ
                     VKLKEAKLLDQVQLRVFDPDERPKLKAGDFMVEPFRVCHSIPDSVGFGITTPVGLIVH
                     TSDFKFDPTPIDGKLTDFELLREFGDRGVKLLISDCVHVEARGTTPSERVVGETYDQV
                     FAQAEGRIIIATFASLIARIQQVIDTAARYERRVAMMGRSLENNVRVATSLGYLKDPE
                     NVIISAKSAKQLPDSEIVYIVTGSQGEPMAVLSRIANNDHPQISVSEGDTVVVSATPI
                     PGNETSVFRIINQLFRAGAEVVYSARALVHVSGHASQDELVQMIELTRPEHIIPTHGE
                     HRHLALYNDLAVGAGIPRNRLHTVEIGDVVVIDRIGVLFDEPIEVGYVYVEGLSVGTM
                     NETVLRDRRTLSNDGMVMIVVSVERRTGKVLAGPEIVTRGFSMSRNGTDVTKGVRDQV
                     LETLAAHAGPQTSDDWSYLSREIREAAASYLYTVTRRRPMVLPMVLEV"
     gene            4037..>6066
                     /locus_tag="VFQ54_11380"
     CDS             4037..>6066
                     /locus_tag="VFQ54_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU0165635.1"
                     /translation="MATQAKGRSNSTSRASSAPAKRKRTTKQQISWWTAMRLRGSAFV
                     HGLIPNLSASDRLKREILGVVLVILALCSSWALGRGNQDGTVISWWSHVLRQSLGVGA
                     PLIPIFAALTAVRAFANQETPILLFRHYAGGTLFAAGVLGMLSFGLNPHGGALGDVIG
                     DIGRGTLGNVASGLVLFAIGVVGVLLLADTNLLALERDIRRLLPKRKKAVVDVAPPAA
                     PLTVKAVVQAKPVPAPKQPRTPVERVINLPKRAKKLDDKVVPSKPLASPTPPGEPMPL
                     PDIRVLAYYDSVEPDSTELETKARTIEETLASFKVEATVREINPGPAVTQFALEPGNG
                     VKVRRIVELQNDLALALAAQSIRVEAPIPGMARVGIETPNAQIAMVGLREVIESAAFT
                     KAKAQLPMPLGRDVNGGYVIGDLVKMPHVLIAGATGAGKSVCLNGIISTLLLTKQPDE
                     LRLLMIDPKMVELTGFNGVPHLQCPVVTEMDKVVGALRMVLREMNRRYELFKKLGVRN
                     FDGYALMREDEPELEKLPHLVVIIDELADLMMTTPDEVETLLARLTQMARATGIHLII
                     ATQRPSVNVLTGLIKANVPSRIAFAVSSMIDSRVVLDMPGAEKLLGRGDMLYLPPGAA
                     KPVRVQGAFIDAEVKAIVDHWHTVAPIPQYAEEWLNLPSSDGPGEESGSGGG"
BASE COUNT         1109 a         1885 c         1992 g         1080 t
ORIGIN      
        1 cgcaaacctg gcgtctactt gatgcacgat cagttcggga acatcatcta cgttggcaag
       61 gcgaagagcc tccgcgagcg cgtctcttcc tattactcac aaccgctcgg ctacaccagg
      121 aagatggacg gcctgctcga atcgatggtc aggatcgaga cgatcgtcgt cggcagcgag
      181 atcgacgccc tgctgctcga ggcgcaactg atccggcgct accagccccg gtacaacacg
      241 gcgatgcgct cgttcgagca ctacccctat attcgcgtcg atgtctccaa tgcctggccg
      301 cggatctcgc tcgccaagac gcggaaagac gatggggcgg aatacttcgg gccctatcgg
      361 agttcgtcgg gcgcgaagcg gacggtggat gtcatcaacg cggcgcttcc gctgcggacc
      421 tgtacccgct cattcaagaa cgcgcggagt tacggcaatc cctgcattct gctcgacatg
      481 ggcaagtgtc tcgggccatg cgtcgggcgc gccgatcgtg acgagtacca gcggatcgtg
      541 cgcgacgtcg tgcaactcct cgacgggcgc gaccaggcgt tgatggagcg gctctgggcc
      601 gaactcgaga tcgccgcgga gaagctggac ttcgagaagg cgggcaagct gcggcgcgac
      661 ctgcgttccg tcctcggtct ggtcgagaac cagcagcgcc tgcgcgaggc ggaacaactc
      721 cataatctgg tcctcgtgct gccgtcgccg gatgtcgcgt tccgcgaact cgtcctgatc
      781 ttcggcgggc ggatctgggc gcgattccgg atgaagcgcg aagcctcgcg cgagggagca
      841 ttcgtgcccg cgatcgtcgc cgacgacacc gttctcagca tcgattcctt tcggaccgga
      901 tcgctccgga aggtcgccga ggatcgcgtg gtctacgggg atggacgaga gatcgatgac
      961 ctggcatcgc ggctcgcgcg cgcggcggag cgccacgcgg agcgtggcga cgatccggtc
     1021 gacaactatc tcgtcgacga gaacaacatc atcaaccggt ggctgatgca gcatgccggg
     1081 catccggcgt tgctgccgct cgccgacgat atggatcgcg gggcctggca ccacctgctc
     1141 atcaccgcgc ttgcgctgac cgatgacgat cttcaattcc gggagcgggt gaggatcgac
     1201 ggcgagggtg aagcggatga gggcggcgat gtgatcgaga cgatcgcgcc gaccgccggt
     1261 cagggtgcat ccgctgccgt gggcatctag cggcgagcgc ggtcagcgcc gcgcttacat
     1321 aaccgtccgc tcccggtgca ggcacggcga ttgcaatcag ccgttcggat cgggttatgc
     1381 ctcgttaggg cgaccgggcg ttggctatac tcacacctga gaatgaatgg cgagcgctcg
     1441 acaagaggga tgacggtgtc ggatcaaggg tcggagtcag cggagaagct ttcggtcatc
     1501 gcgagtgaag tgaaagtctg cacgctgtgc gaactctcgc ggacgcggac gaatgccgtg
     1561 ccgggcgagg gagatgccaa cgcccgcgtg atgctgatcg gggagggtcc cggttggcat
     1621 gaggaccagg aaggccgccc gttcatcggc aatgccggca agttccttac cgagcttctc
     1681 gagaaagccg gcctccggcg cgaagacgtc ttcatcacga acgtcgtgaa gtgccggccc
     1741 cccagcaacc gcgatccgtt gcccgacgag attcatgcct gctcgctcta cctcgagcgc
     1801 cagatcgcca cgatcgatcc cgacgtgatc gtcaccctcg ggcgcttctc gatggcgaag
     1861 tatttccccg gtgaacgcat ttcccggatc cacggtcagg caaagaagct tggcaatcgg
     1921 ctcgtcgtcc ccatgtatca tcccgccgcc gcccttcacc agggcaacct ccggggcgag
     1981 atcgaggaag acttcgccaa gctcccgaag cttctcgccg agcgggaacg cgaacgcgac
     2041 gccgaggtcg agcgggaggc cccgccgcgg cagatttccc tcttctagaa cccctccgag
     2101 aaaggccgac cattctgtgg gtgaaccaac cgtcaagctc atctttctcg gtggtatcgc
     2161 ggaagtcggc aagaatctct ttctcctgga gacggatgat gacatcatcg tcgtcgactg
     2221 tggattgggt tttcccgaag aggatcagct cggaatcgat ctcgtactac cggacatctc
     2281 ctatctgcgg gagcggcgcg agaagattcg cggcatcttc atcacccacg gtcacgagga
     2341 tcacatcggc ggtatcgcct acctctggcc cgatctcggc gcccccgtct acgcgacgcg
     2401 cctcaccgcc ggtctgatcc aggtcaagct gaaggaggcg aagctgctcg atcaggtgca
     2461 gcttcgtgtc ttcgatccgg acgagcgccc gaagctgaag gccggcgact tcatggtcga
     2521 gccgttccgg gtctgccaca gcatcccgga ttcggtaggg ttcgggatca cgacgccggt
     2581 cggcctgatc gtccacacgt ccgacttcaa gttcgacccg acgccgatcg acggcaagct
     2641 gaccgatttc gagttgctgc gggaattcgg ggatcgcggc gtgaagctgc tgatctccga
     2701 ttgcgttcac gtcgaagcgc gggggacgac gccttcggag cgggtggttg gcgagacgta
     2761 cgaccaggtc ttcgcgcagg cggaagggcg gatcatcatt gcgacgttcg cctcgctgat
     2821 cgcccggatc cagcaggtga tcgatacggc tgctcgctac gagcggcgcg tggcgatgat
     2881 gggacggagc ctcgagaaca atgtccgtgt ggcgacctcg ctgggctacc tcaaggatcc
     2941 cgagaatgtc atcatctcgg cgaaatccgc caagcagctg cccgatagcg aaattgtcta
     3001 catcgtgacc ggaagccagg gcgagccgat ggcggtcctg agccggattg cgaacaacga
     3061 tcatccccag atcagcgtca gtgaaggcga caccgtcgtc gtctcggcga cgccgatccc
     3121 gggcaacgaa acctcggtct tccggatcat caaccagctg tttcgcgccg gtgccgaagt
     3181 ggtctactcg gcgcgagcgc tggtgcacgt ctccgggcat gcgagccagg acgaactcgt
     3241 gcagatgatc gagttgacgc ggccggagca catcatcccg acgcacggcg aacatcgtca
     3301 cctcgcgctc tacaacgatc tcgcggtcgg ggccgggatc ccgcgcaatc gcctccacac
     3361 ggtcgagatc ggcgatgtcg tcgtgatcga caggatcggc gtgctcttcg acgagccgat
     3421 cgaggtcgga tacgtctatg tcgagggcct ctccgtcggg acgatgaacg agaccgtgct
     3481 tcgcgatcga cggacgctct cgaacgacgg gatggtgatg atcgtcgtca gcgtcgagcg
     3541 gcggacgggc aaggttcttg ccggtcccga gatcgtgact cgcggcttct cgatgagtcg
     3601 caacgggacg gacgtcacga agggggttcg cgatcaggta ttggagacgc tcgcggccca
     3661 tgccggtccc cagacgagcg acgactggag ttacctgagc cgtgagatcc gcgaagccgc
     3721 ggcgtcgtat ctctacaccg tgacccgccg ccgcccgatg gtgcttccga tggtcctcga
     3781 ggtctagaac ttccccagga acaggcacga ttccacggcg acccgattcg ttcgggccgc
     3841 cgtttcgacg tgtcgcgacc gacccggtgt gcggcgaaaa gccctatcga gtcccgggtt
     3901 gacagatggt catgcatgtc cggacggtgg tgtataatgt gttcgtacac cccttcggcg
     3961 cggtcgagac agcccaactg actcaccggc atgcatcggg cgtgtgggat ggatagcgaa
     4021 gatggagtga ggggccgtgg ccacacaggc gaagggccgg tcgaatagca cgtcgagggc
     4081 gagttcggct ccggcgaaac gcaagcgcac gacgaaacag cagatttcgt ggtggaccgc
     4141 gatgcgattg cgcggctcgg cgttcgtcca cgggctcatt cccaacctct ccgcatccga
     4201 tcgtctcaag cgggagatcc tgggtgtcgt gctcgtcatc ctcgcgctct gtagctcctg
     4261 ggccctcggt cgcggcaatc aggatgggac cgtcatttcg tggtggtcgc acgtcctgcg
     4321 gcaatcgctt ggtgtcgggg cgccgctgat accgatcttc gcggcattga ccgcggtccg
     4381 cgcatttgcg aatcaggaga cgccgatcct gctcttccgg cactacgccg gggggacgct
     4441 cttcgccgcg ggcgtgctcg ggatgctctc cttcggtctg aatcctcatg gcggagccct
     4501 cggtgacgtg atcggtgaca tcggtcgcgg cacgctgggt aacgtggcgt cgggcctcgt
     4561 gctcttcgcg atcggcgtcg ttggagtcct gcttctcgcc gacaccaacc tccttgcgct
     4621 cgagcgtgat atccgcaggt tactgccgaa acgaaaaaaa gcggtcgttg acgtcgcacc
     4681 accggcggcg ccactgacgg tcaaagcggt ggtgcaggcg aagcctgtcc cggcgccgaa
     4741 gcagcccagg actcccgtgg agcgggtgat caacctgccg aaacgcgcga agaagctgga
     4801 cgacaaggtc gttccttcca agccgctggc gtcgccgacg ccgcccggcg agccgatgcc
     4861 gttgccggac attcgcgttc tcgcctacta cgattcggtc gagcccgatt ccacggaact
     4921 ggagaccaag gcgcgcacga tcgaggagac gctggcgagc ttcaaggtgg aggccacggt
     4981 tcgcgagatc aatccggggc cggccgtgac gcagttcgcg ctggagccgg ggaacggcgt
     5041 gaaggtgcgc cggatcgtgg aattgcagaa cgatctcgcg ctggcgctcg ccgcccagag
     5101 tattcgcgtc gaggcgccga tcccgggaat ggcgcgcgtc ggaatcgaga cgcccaacgc
     5161 ccagatcgca atggtcgggc ttcgcgaggt gatcgagtcc gccgccttca cgaaggcgaa
     5221 ggcgcagctg ccgatgccgc tcggtcgcga cgtcaatggc ggctacgtga tcggtgatct
     5281 cgttaagatg ccgcacgtgc tgatcgctgg cgcgaccggg gcaggcaagt cggtctgtct
     5341 caacgggatc atcagcacct tgctgctgac gaaacagcca gacgagctgc gactcctgat
     5401 gatcgacccg aagatggtcg aacttaccgg cttcaacggc gttccccacc tccagtgtcc
     5461 cgtcgtgacc gagatggaca aggtggtcgg tgcgctccgg atggtcctcc gggagatgaa
     5521 ccggcgctat gagctcttca agaagctggg cgttcgcaac ttcgacggct acgcgctcat
     5581 gcgtgaggat gaacccgaac tcgagaagct gccgcatctc gtcgtgatca tcgacgagct
     5641 cgccgacctc atgatgacga cgccggacga agtcgagacg ctcctggccc gcctgacgca
     5701 gatggcgcgg gcgaccggaa tccatctcat catcgcgacg cagcgcccgt cggtgaacgt
     5761 gctcacgggt ctgatcaagg cgaacgtgcc gtcgcggatc gccttcgcgg tctcgtcgat
     5821 gatcgacagc cgcgtcgtgc tcgacatgcc tggcgcggaa aagctgctgg gacgtggcga
     5881 catgctctac cttcctcccg gtgcggcgaa gcccgtacgc gtgcaagggg cgttcatcga
     5941 cgccgaggtg aaggctatcg tcgaccactg gcacacggtg gccccgatcc cgcagtacgc
     6001 ggaggaatgg ctcaacctgc caagcagcga cggaccggga gaagagagtg gcagcggtgg
     6061 tggcga
//
LOCUS       DASTMD010000768         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1501492, whole genome shotgun sequence.
ACCESSION   DASTMD010000768 DASTMD010000000
VERSION     DASTMD010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501492"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      410..523
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            516..1355
                     /locus_tag="VFQ54_11385"
     CDS             516..1355
                     /locus_tag="VFQ54_11385"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0165636.1"
                     /translation="MHPPDPALRDDVPRLRLSHIDSHYAEQGRTLEALRDISLDVRDG
                     EFVALIGPSGSGKSTLLDIVAGLFAQTGGDVALDGDVLPLKYRLGRTAYMRQRDLLLP
                     WRSALDNAALALEVHGVPRRDARQIAADRFPEFGLAGFEKAYPAQLSGGMRQRVAFLR
                     TVLADRPLLLLDEPFGALDALNRVALQTWLLDLWERERRAVLLVTHDVDEAIFLADRV
                     IVFSARPGQIAHIEPIDLPRPRRREIVTSPRFLEHRVALLDALGLLHQDQPLGRTMEG
                     ALA"
     gene            1352..2131
                     /locus_tag="VFQ54_11390"
     CDS             1352..2131
                     /locus_tag="VFQ54_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165637.1"
                     /translation="MSSLLRSLNRWIWPLLILALLIAFWEIAVRVENTPRWQLPPPSA
                     VFDSFRDDHALLLRNARVTLGEVLLGFGFALVAGILVGIAIDASRILERAIYPLIIAS
                     QTIPMVALAPLLLIWFGYGLTPKVIVTALVCFFPIAVNTVDGLRAADREMLALLRSMG
                     ASAWQRFRLVKIPAALPSLFTGARIAIAFSVIGAVFGELVGSSEGLGYLMQRSAAQFQ
                     TARVFAAIVILSAMGVGLFAIVTLLERLVLPWRRLVTDTNS"
BASE COUNT          323 a          756 c          658 g          420 t
ORIGIN      
        1 gctcggcgcg aacgctgttc gtaccgctcg cggtcggtcg cgcggaagtt cctgtcgttt
       61 gtctggtcgt ttccacgtcc gtgtgaagaa cgatcgtaat catcagtcgt ctcccttgct
      121 ggccgaccgc gacgggagta cacgccccga tcgcaacgag attggttggc aagagccata
      181 ctagcggacc gtccgtagaa aatcaatacc aatatcaatg aaatcaatgt caatcgaaat
      241 attctcaatc gcagccctgg agatcgacga ggaacggctg cgagattctt gaggacacgt
      301 cgcgacagaa tcaaggtacc ggcttgattt cctccgcccc ggcacgcccc ggcgagcgat
      361 cgccagcccg tttcccgcgg gatgccgcgt gtggcatcat gtgcggatat ccacgagctg
      421 cgggagcccg ggtgagccgg gctgagatcg cgaccgtcct ccgtcgccga ccgctgaacc
      481 tgacccgggt catgccggcg taggaaggct accgaatgca tccccccgat cccgcccttc
      541 gcgacgacgt gcccagattg cgcctctcgc acatcgactc ccactacgcc gagcagggcc
      601 gtacgctgga agcgctccgc gacatcagcc tggacgtgcg cgacggcgag ttcgtcgccc
      661 tgatcggacc gtccggctcc ggcaagagca ccctgctcga catcgtcgcc gggctcttcg
      721 cgcagaccgg cggcgacgtc gcgctcgacg gcgacgttct tccactgaag taccgcctgg
      781 gtcggaccgc ctacatgcgg cagcgcgatc tcctcctccc ctggcgctcg gcgctggaca
      841 acgcggcgct cgcgctcgaa gttcacggcg ttcctcgtcg tgacgcgcgg cagatcgccg
      901 ccgatcgctt ccccgaattc gggctcgccg gcttcgagaa ggcctatccg gcgcaactct
      961 ccggcggcat gcgccagcgc gtcgccttcc tccgtaccgt ccttgccgat cgcccgctcc
     1021 ttcttctcga cgagcccttc ggcgcgctcg atgcgctcaa ccgcgtcgcg ctccagacct
     1081 ggctgctcga cctctgggaa cgggaacgcc gcgcggtgct gctcgtcacc cacgacgtcg
     1141 acgaggcgat cttcctcgcc gaccgcgtga tcgtcttctc ggcgcgaccg ggccagatcg
     1201 cccacatcga gccgatcgac ctgccacgcc cgcgtcggcg cgagatcgtc acctcgcctc
     1261 gctttctcga acatcgtgtc gcgctgctcg atgcgctggg attgctgcat caggatcaac
     1321 ctctcggcag gacgatggaa ggagccctcg cgtgagttcg ctccttcgct ccctcaaccg
     1381 ctggatctgg ccgctgctga tcctcgcgtt gctgatcgcg ttctgggaga tcgcggtccg
     1441 cgtcgagaac acgccgcgct ggcagttgcc accgccatcg gcggtcttcg actccttccg
     1501 ggacgatcat gccttgctcc tgcgcaacgc gagggtcacg ctgggcgaag tcctgctggg
     1561 gttcggcttc gcactcgttg cggggattct ggtaggaatc gcgatcgacg cctcgcggat
     1621 tctcgagcgg gcaatctatc cgctcatcat tgccagccag acgattccga tggtcgcgct
     1681 cgcaccgctg ctgctgatct ggttcggcta cggcctcact cccaaggtaa tcgtgacggc
     1741 gctcgtctgc ttcttcccga ttgcggtcaa tacggtcgac gggctgcgcg ccgccgatcg
     1801 cgagatgctg gcgctgctcc ggtcgatggg cgcgagcgcc tggcagcggt tccggctggt
     1861 gaagatcccg gcagccctgc catccctctt caccggggcg cggatcgcaa tcgccttcag
     1921 cgtgatcggc gccgtcttcg gtgagctggt cggctcgagt gaaggtctgg ggtatctgat
     1981 gcagcgctcc gccgcccagt tccagacggc gcgtgtcttc gcggcgatcg tcatcctctc
     2041 ggcgatgggt gtcgggctgt tcgcgatcgt gaccttgctc gagcggctcg tcctcccctg
     2101 gcgtcgcctc gtgaccgaca ccaacagctg atccgcatct catccagacc ggggata
//
LOCUS       DASTMD010000769         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2839408, whole genome shotgun sequence.
ACCESSION   DASTMD010000769 DASTMD010000000
VERSION     DASTMD010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2839408"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VFQ54_11395"
     CDS             <1..815
                     /locus_tag="VFQ54_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0165638.1"
                     /translation="RLQIFSDLHVDVDAVRPVVIGTDVDLVVVAGDTAEGATRAFQAL
                     RRIVPEPITIAMTMGNHEFYGSVHEDELAVAREAAPAFNVVLLENEVAVIGGCRLAGA
                     TLWSDYRLFGPVNAPAAMHAARVEMNDHRRIRWSKKPWRRFRPEEALSLHGRSRAFLA
                     DVVATPFDGPTVIVCHHAPHIRSVGERFSSDVLSAAFASDALRALFPQDNRIRAPPGD
                     GNAEKRVELWIHGHVRHSVDYEIAGIRVLANPHGYGEENPEFNPQLIIEVKS"
     gene            812..1306
                     /locus_tag="VFQ54_11400"
     CDS             812..1306
                     /locus_tag="VFQ54_11400"
                     /inference="COORDINATES: protein motif:HMM:NF042721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6634 family protein"
                     /protein_id="HEU0165639.1"
                     /translation="MNRRIRRKQPPVHQQARPPPPATGARSSGRPYVQKTKQTMTMNS
                     DFLNPNLLDCEMPNRLHRLADDIRKILDGTGPSTETLAGAPLLENWRYVASKLGARLA
                     GNVVGHPRLRGGAIVTSPAWVTDPQERWCRTTSRYYRLGKRVDRDDELSNDLFTDDFN
                     GDLR"
     gene            1303..>2000
                     /locus_tag="VFQ54_11405"
     CDS             1303..>2000
                     /locus_tag="VFQ54_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165640.1"
                     /translation="MKREETTFADRSDMGPLEAAVYRAERAAASKFRRHHIAGPEASD
                     PDAGDDENSQLFGHADEIAIGGFEAVRRHLATTVDDDVEERIIDRKLAPPMPGRIAAR
                     LLLARLFDSAPTVIGRILSETPVVIVDVPDDDLYNRVASSWKEVLGLDQLRFLDISKL
                     SDETVRTGFGAISAVYDKPMMKSDRIAADARAYHAVQIALPIVAITPAAETHLSKVLL
                     DAATDRLTLSGIDGR"
BASE COUNT          384 a          636 c          613 g          367 t
ORIGIN      
        1 tgagactgca gatcttctcc gatctccacg tcgacgtgga tgcggtccgg ccggtcgtca
       61 tcggcactga cgtcgatctt gtcgtcgttg ccggcgacac ggcagagggc gctacccggg
      121 cattccaggc tttgcggcgg atcgtgcccg agccgatcac gatcgccatg acgatgggaa
      181 atcacgaatt ctatgggagt gtccatgaag acgaactcgc ggtcgccaga gaagcggctc
      241 ccgccttcaa cgtcgtactg ctcgagaatg aggtcgcggt gatagggggc tgtcgtcttg
      301 ccggtgcgac gctctggtcg gactatcggc tcttcggtcc cgtcaacgct ccggcagcaa
      361 tgcacgcggc gcgggtcgaa atgaacgatc accgcaggat caggtggagc aagaagccgt
      421 ggcgacgatt ccgcccggaa gaggcgttat cgctgcatgg ccgctcgcgt gcgttcctcg
      481 cagacgtcgt ggcgaccccg ttcgacggcc cgaccgtcat tgtctgtcat catgctcccc
      541 acattcgttc cgtcggcgag cgtttttcca gcgacgtcct gagcgccgca ttcgcaagcg
      601 atgcgcttcg tgcgttgttt ccccaagaca atcggatccg ggcgcccccc ggggacggca
      661 acgcggaaaa gcgtgtcgag ctttggatcc acggccacgt gcgccacagc gtcgactacg
      721 aaatcgctgg gatcagggtc ctcgccaatc cgcatggata cggtgaggaa aatccggagt
      781 tcaatcctca actcatcatc gaggtgaagt catgaacagg cgcattcgcc gcaaacagcc
      841 ccctgtccac cagcaggcaa ggcctccacc ccccgccacc ggagcgagaa gctccggccg
      901 gccatacgtt cagaaaacaa agcaaacgat gaccatgaac tccgatttcc tgaacccgaa
      961 cctgctcgat tgcgaaatgc cgaaccgcct gcaccgcttg gctgacgaca tccgcaagat
     1021 actcgacggc accggtcctt caaccgagac gctcgccggc gcgcccctgc tcgagaattg
     1081 gcggtacgtg gcctccaagc tcggtgctcg cctcgcgggc aacgtggtcg gacatccgcg
     1141 tcttcgcggg ggagcgatcg tcacatctcc ggcctgggtc acggacccgc aggagcggtg
     1201 gtgcaggacg acctcacgct actaccggct cggcaagcgc gtcgaccgcg atgacgagct
     1261 gtcgaatgat cttttcaccg atgactttaa cggggatttg cgatgaaacg cgaagagacg
     1321 acttttgctg atcgatcgga catggggccg ctcgaggcag cagtctaccg cgccgaacga
     1381 gctgccgcct ccaaatttcg tcggcatcat atcgcaggcc cggaggcatc ggatccggat
     1441 gccggtgacg acgaaaattc gcagctcttt gggcacgcgg acgaaatcgc gataggcggc
     1501 ttcgaagctg tgcgacggca cctggctacg acggtggacg atgacgtcga ggagaggatt
     1561 atcgaccgca agctggcgcc acccatgccg ggccgtatcg ccgctcgcct gctgttggca
     1621 cggctattcg actccgcgcc gacggtcatc ggtcgaatcc tgtccgagac ccctgtcgtg
     1681 atcgtcgatg tgcctgacga cgatctctac aaccgcgtcg cctcctcttg gaaggaggta
     1741 ttgggcttgg atcagttgcg tttcctcgat atttcgaagc tctccgacga gacggtgcgc
     1801 accgggttcg gcgcgatcag cgccgtttac gacaagccga tgatgaagag cgacaggatc
     1861 gctgcggacg cgcgcgcgta tcacgcagtg cagatcgccc tgccgatcgt cgcgatcacg
     1921 ccggcagcgg aaacgcacct ttccaaggtg ctcctcgacg ctgccaccga caggttgacc
     1981 ctctcgggga tcgacgggcg
//
LOCUS       DASTMD010000770         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2304068, whole genome shotgun sequence.
ACCESSION   DASTMD010000770 DASTMD010000000
VERSION     DASTMD010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304068"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..601)
                     /locus_tag="VFQ54_11410"
     CDS             complement(116..601)
                     /locus_tag="VFQ54_11410"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEU0165641.1"
                     /translation="MTERVETIYLDADACPVKEYVFRVAARYTVPLVVVANTPIRIPD
                     ERGLSARLVVVPDSPDAADDWIVENAVKGDLVLTADIPLAARVIGNGVDALDFRGGAY
                     NPNRIGDAVASRDLNAHLRSVGLVTGGPSAFSQKDRSKFASTLDAAVGKMKRRAERRG
                     I"
     gene            720..>2746
                     /locus_tag="VFQ54_11415"
     CDS             720..>2746
                     /locus_tag="VFQ54_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU0165642.1"
                     /translation="MMPIIPAIAPDPRVDPQSITPTDVSQFIRLDQCERYLRLRLHER
                     ATGSGFMREYDVVPQAIPPILTRSGAEFEATIERDLGYYFPLVKFTEAGRQARGQTTD
                     NQAVSELARDLAPGEAKICLQPRLEAILNGWKIRGDVDILRLERDEEGTLTVLIADMK
                     ASTAAKIEHRLQVAFYHELLTHIFAATGIAHGPIELAILYRGPAATEDGENPAEVDEL
                     HIAQQELAFELFGTRAGLLERIDDAQPYVDSVRDLVIGSQSTARRVLATPFDEIPFHL
                     TYKCDGCLFNEFCMNASAETDDLSLLPHITEQDKGVLRREGIATVRELATLKELRRKG
                     QIQVDGEVQEETILVPAPAKDALSRRLAATWPVGPRIDELIHRAKRYRKWIKDPMEAL
                     DWIPHKGYGSLPWSDETQNPNLVRVFIDAQHDYLHDRLYMAGALVVGNEGGFPNPARR
                     RSAVSLSDAPPDSAAIEERLLVDWIANTLQAVVEVAAPDPEGNARAPIHLVFINDFAQ
                     QVLLAALGRHASTILGATALYDFVTQLAAFDSPLITFLDRQIREQKNYPMICQSLQAV
                     AAFRHFDWNEGIPYRKLFRARMFDFWGRLDEPDDDFRAESWFTNRARFNSQIPLEFAY
                     NAWGELPRRDDASTDEMAPYRTSTPALLAGFHGRRLEAMEWIANDFRGNKQT"
BASE COUNT          507 a          997 c          801 g          441 t
ORIGIN      
        1 gaaggggcgc cagacgagca tacggagcca cgtatcgttc gcgcttcttc tcgcttgcgt
       61 cgatcgagct ggcacgatgg cgagcaggaa cgcgggtact gttccagacg tggcttcaga
      121 tcccgcgacg ctccgcgcgg cgcttcatct tcccgacggc agcatccagc gtcgaggcga
      181 acttgctccg atccttctgc gagaaggcgg acggcccgcc cgtcaccagc cccaccgagc
      241 gcagatgcgc gttcagatcg cgcgatgcca ccgcatctcc gatccggttc gggttgtagg
      301 cgccgccgcg gaagtcgagc gcatccaccc cgttgccgat cacgcgcgcc gcgagcggga
      361 tatccgcggt caacacaagg tcgcccttca cggcgttctc gacgatccaa tcgtcggcgg
      421 catccggtga atcgggcacg acgacgagac gcgcactgag accgcgctca tccggaatcc
      481 ggatcggcgt gttcgcgacc acaaccaacg gcaccgtgta tcgcgccgcg acccggaaga
      541 catactcctt caccgggcag gcatccgcat ccagatagat cgtctccacg cgttccgtca
      601 ccaggcatcc tccataagaa cgatatatcg acaccgatcg tcccgatcag gcattatgtc
      661 cggaacgccg cttcgcattg ggacccatcg caagcaaccc cagagcctgg atcgatatca
      721 tgatgcccat tatccccgcg atcgcgcccg atccacgggt tgatccccag tccatcaccc
      781 cgaccgatgt ctcccagttc atccggctcg accagtgcga gcgctatctt cgcctccggt
      841 tgcacgaacg cgccaccggc agcggtttca tgcgcgagta tgacgtcgtt ccgcaggcga
      901 tcccaccgat cctgacgcgg tccggggccg agttcgaggc gaccatcgag cgcgatctcg
      961 ggtactactt cccgctcgtc aaattcaccg aggcgggccg ccaggcgcgc ggccagacga
     1021 ccgacaacca ggccgtctcc gagctggcgc gcgacctcgc cccgggcgag gcgaagatct
     1081 gcctccagcc gcgcctcgag gcgattctca atggctggaa gatccgcggc gacgtcgaca
     1141 tcctgcgcct cgagcgcgac gaagaaggta cgctgaccgt cctcatcgcc gacatgaagg
     1201 cgtccaccgc ggcgaagatc gagcatcggc tgcaggttgc cttctatcac gaactgctga
     1261 cgcacatctt cgccgcaacc gggatcgcgc atgggccgat cgagctcgcc atcctctacc
     1321 ggggtcccgc cgcgaccgag gacggcgaga atcccgccga ggtcgacgag ctccacatcg
     1381 cccagcagga actcgccttc gagctcttcg ggacgcgagc cgggctcctc gagcgaatcg
     1441 acgatgccca accctacgtc gactccgttc gcgatctcgt gatcggctcc cagtcaacgg
     1501 cgaggcgcgt cctcgcgacg ccgttcgacg agataccctt ccacctcacc tacaaatgcg
     1561 acggctgcct cttcaacgaa ttctgcatga atgcgagcgc ggaaacagac gacctctccc
     1621 tgcttcccca catcaccgag caggacaagg gcgtgcttcg gcgcgaaggc atcgccaccg
     1681 tccgtgagct ggcgacgttg aaggagctcc ggcgcaaggg ccagatccag gtcgatggcg
     1741 aggtgcagga ggagacgatc ctcgttcccg cgccggcgaa ggacgcgctc tcgcggcgtc
     1801 tcgcggcgac gtggccggtc ggtccgcgaa tcgacgaact gatccaccgc gcgaagcggt
     1861 atcggaaatg gatcaaggat ccgatggaag cgctcgactg gatcccccac aagggatacg
     1921 gctcgctgcc ctggagcgac gagacccaga atccgaatct cgtccgcgtc ttcatcgacg
     1981 cccagcacga ctatctccac gatcgcctct acatggccgg cgccctcgtc gtcgggaacg
     2041 agggcggatt cccgaacccg gcccgccgcc ggagcgccgt cagcctcagc gacgcgccac
     2101 cggactctgc cgcgatcgag gaacggctcc tcgtcgactg gatcgcgaac acgctccagg
     2161 cggtcgtcga ggtcgccgca cccgatcccg aggggaatgc ccgcgccccg atccacctcg
     2221 tcttcatcaa cgacttcgcg cagcaggtcc ttctcgccgc cctcggtcgc cacgcctcga
     2281 cgatcctcgg cgcgaccgcg ctctacgact tcgtgaccca gctcgccgcc ttcgattccc
     2341 cgctgatcac cttcctcgat cgccagatca gggagcagaa gaactacccg atgatctgcc
     2401 agtcgttgca ggccgtcgcc gcgtttcgac acttcgactg gaacgaggga atcccctacc
     2461 gaaagctctt ccgcgcccgg atgttcgact tctggggtcg actcgacgag cccgacgacg
     2521 acttccgcgc cgagagctgg ttcaccaacc gcgcccgttt caacagccag atcccgctcg
     2581 agttcgccta caacgcctgg ggcgaactcc cccgtcgcga cgatgcgtcc accgacgaga
     2641 tggcgcccta ccgcacctcg acgccggcgc ttctcgcggg gttccacggt cggcgcctgg
     2701 aagcgatgga atggatcgcc aacgatttcc gcggcaacaa gcagac
//
LOCUS       DASTMD010000771         4175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2662360, whole genome shotgun sequence.
ACCESSION   DASTMD010000771 DASTMD010000000
VERSION     DASTMD010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2662360"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..816
                     /locus_tag="VFQ54_11420"
     CDS             304..816
                     /locus_tag="VFQ54_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0165643.1"
                     /translation="MRRFNPRKRQRMLGYDYSLSNSYFVTFTSEHRTSRFGDIHDGRV
                     HLNEPGQMVDAVWRSAPSHHPSLQLDTFVVMPDRIHPLIHLSEEFEERPTVSSLVQWF
                     KTLTTRRYITGVKEHGWAPFDGRLWKMGFHDRIVRDGEELDHIRAYIEDNPRRRWLTM
                     SEDRVFPEVQ"
     gene            complement(1120..1800)
                     /locus_tag="VFQ54_11425"
     CDS             complement(1120..1800)
                     /locus_tag="VFQ54_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU0165644.1"
                     /translation="MRGLLLDVYGTLVHEDDVSIADITGHIHRYAGVEVDPETIGRAW
                     SAAFFAMCDRSFGANFLTQRDLALIALKEVLDAYKAPLDASLLLERQIAHWIAPPIFA
                     DAIPFLDRVAALGIPICIVSNIDRADVEAALGYHGLSFEHIVTSEDARAYKPRPEMFD
                     MAAARLGLAPVELLHVGDSRRSDVVGAGAAGIPVAWVNRTGKTASGDPAADHVVSDLT
                     ELARLLGW"
     gene            1953..3431
                     /locus_tag="VFQ54_11430"
     CDS             1953..3431
                     /locus_tag="VFQ54_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU0165645.1"
                     /translation="MTTNQSSTTRSETPTLGFTVVERLGPGQRDAVRRLADVYARAGA
                     ELYLVGGIVRDVLLQRPVPNDLDFATSARPEQTKALVKSMPHVGLYDIGEKFGTIGLI
                     LPGPDAGDEPVTIEITTFRTEQYETGSRHPTVVYGTSIEDDLARRDTTINAIAVDVHT
                     GRLIDPFFGQADLAAGLLRAVGDPDERFAEDPLRLLRIARFVSQLGFAVESETKAAMA
                     RQAPTLAQISKERILAELTKLLCGEYADAGLETLLETGLFTIAMPELAPLERSAWNHP
                     GVHREKDLWDHTKRVVIQSPQRPVVRWAALLHDAAKPLTRSVDSHGEVHFFGHERVGA
                     DLASKLLRRLNADKQTQSQVRRLVDLHLRPASYDTVTWTDSAVRRLALEAEDVLEDLL
                     DLAAADVTSERAAKRRAAATRVQGLRDHIARLEEEQALDQLQSPLDGNELMQMFDRKP
                     GKWIAEVKNHLRELVIDGELSSDDKETATRIARDLLAAEPAP"
     gene            3503..>4175
                     /locus_tag="VFQ54_11435"
     CDS             3503..>4175
                     /locus_tag="VFQ54_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0165646.1"
                     /translation="MSTITSTDSSPRDDAKERVKAQYGSVGDAYVTSKDHAGGDDLAR
                     MVEVANPQPTELLIDIATGGGHVAKTFAPHVAKAIATDLTPEILLHAGNFFAELGLTN
                     VETKLADAEELPFPDASADIVTCRIAPHHFPHPDRFVQEVTRVLRPGGRFVLIDSTVP
                     AGEAALFFNRFEKLRDPSHVRSLTADEWDLLITSTGLTIEIVEDFPKRHDFDDWTARS
                     RMTAED"
BASE COUNT          825 a         1435 c         1221 g          694 t
ORIGIN      
        1 gtcgagtggg cggcgaggcg gcgcgagatt cctgcaggga acggtcgtcg catgccaagg
       61 cacgtcgagt gggcagcggg aggcgggaca ttacgccatc caaagaccgg atatcgtgca
      121 gcaaaggcgc tctgctggcg gtctttggac tccgcagtcc cgccctacgt acgaacgccg
      181 cttgaacggt ggcctggacg ggagcgagga cacgcagcgg gattgccctg cctcgctacg
      241 cgcacgcaac gatacaatgg gtaggtttcg acctcgccca acctggcccc taaaggagtt
      301 tccatgcggc ggttcaatcc tcggaaacgc caacgcatgc tcgggtacga ctacagtctc
      361 tcgaattcct attttgtgac gttcacttcc gaacatcgca catccagatt tggtgacatt
      421 cacgatggtc gggtgcatct gaacgaaccc gggcagatgg ttgatgccgt atggcggtcc
      481 gcaccgagcc atcatccttc gctgcaactc gacacgtttg tggtcatgcc agaccgtatc
      541 catcccctca tccatctgtc cgaagagttt gaggaacgcc cgacggtctc cagcttggtg
      601 caatggttca agacgctcac gactcgccgc tacataaccg gcgtgaaaga gcatggatgg
      661 gcaccattcg acgggcgttt atggaagatg ggatttcacg atcggatcgt gcgcgacggc
      721 gaggaactcg atcacatccg cgcatacatc gaggacaatc cgcgtcgccg atggctgacg
      781 atgtcggagg atagggtctt cccagaagtg caatgacatc gaatcgagga catgaggaaa
      841 gcgttcgtgc gtagggcggg actgcggagt ccaaagaccg gcattcaggg tgtctttgga
      901 gcaggacgcc cggtctttgg atggcgtaat gtcccgcctc ccctcgccca accgacgcgc
      961 ccttgcaccg gatgtccggt ccccgcagga atctcccgcc tcctctccac ccactcgacg
     1021 cgcctttgca tgcgacggcc ggtccctgca ggaatctccc acctacacgc ttctgatcgc
     1081 aactttcgca ctggtactgg cgaacggatc gggcggcgat caccacccga gaaggcgcgc
     1141 cagttcagtc agatcgctca ccacatgatc tgcagcggga tcaccactcg cggtcttccc
     1201 ggttcgattc acccacgcca cagggattcc tgccgcaccg gccccgacaa cgtcgctccg
     1261 gcgtgagtct ccgacgtgaa gcagttcgac tggtgccaat ccgagacgag ccgcggccat
     1321 atcgaacatc tccggtcgcg gcttgtaggc gcgcgcgtcc tcgctcgtca cgatgtgctc
     1381 gaacgacaat ccgtgatagc cgagcgccgc ctcgacatca gcgcgatcga tattcgagac
     1441 gatgcagatc ggaatgccga gcgcggcgac ccgatcgagg aacgggatcg cgtcggcgaa
     1501 gatcggcggc gcgatccaat gcgcgatctg tcgctccagg agtagcgatg cgtccagcgg
     1561 ggccttgtag gcatcgagga cctccttcag agcgatcaac gcgagatccc gctgggtcag
     1621 aaagttcgca ccaaacgagc gatcgcacat cgcgaagaag gctgcagacc acgcccgccc
     1681 gatcgtttca ggatcgacct cgaccccggc gtaccggtgg atgtgcccgg taatgtccgc
     1741 gatactgaca tcgtcctcgt gcaccagcgt tccatagacg tcgagcaaca acccgcgcac
     1801 ctgcgtcaat cgagcctccc aacaccacag ccgtcccgga aaaccccgat cctgccacgc
     1861 ggaaacgagt cgtacaatag gcagcgacaa atacgggccg cggcttcccg agccgcgcca
     1921 caacgacgac tgcagacagg cacacccttc gcatgacgac caaccaatct tccacaacca
     1981 gatcggagac gccgacactc ggtttcaccg tcgtcgaacg cctcggacca ggacagcgcg
     2041 atgccgtgcg ccggctcgcg gacgtctacg cgcgtgccgg agccgagctc tacctcgtcg
     2101 gcgggatcgt ccgcgatgtc ctcctgcaac gaccagtccc gaacgatctc gacttcgcca
     2161 cctcggcacg acccgagcag acgaaggcgc tcgtcaagtc catgccacac gtcgggctct
     2221 acgacatcgg cgagaagttc ggaacgatcg gtctcatcct gcccggcccg gatgccggcg
     2281 acgagccggt cacgatcgag atcacgacct tccgcacgga acagtacgag accggcagcc
     2341 gccacccgac cgtcgtctac ggaacctcga tcgaggacga cctcgcccgt cgcgatacga
     2401 cgatcaacgc gatcgccgtc gacgtccaca ccgggcgcct gatcgatccc ttcttcggac
     2461 aggccgacct cgcggcgggt ctgctgcgtg ccgttggtga cccggacgag cgcttcgccg
     2521 aggatccgct ccggctcctt cgcatcgccc gcttcgtctc ccagctcggc ttcgccgtcg
     2581 aatccgagac gaaggcggcg atggcgcgcc aggcgccgac gctggcccag atcagcaagg
     2641 agcgcatcct tgccgaactg acgaagctcc tctgcggtga gtacgcggat gccggtctcg
     2701 agacgctcct cgagaccggg ctcttcacga tcgcgatgcc ggaactcgcg ccgctcgaac
     2761 gaagcgcctg gaatcatccc ggcgtgcacc gcgagaagga tctctgggat cacaccaagc
     2821 gggtggtgat ccagtcaccc caacgacccg ttgtccgctg ggcggcgctc ctgcacgacg
     2881 ccgcgaagcc actcacccgc tccgtcgatt cccatggcga agtccatttc ttcggacacg
     2941 aacgcgttgg cgccgatctc gccagcaagt tgctgcgacg tctcaacgcg gacaagcaga
     3001 cgcagagcca ggtccgccgc ctggtcgatc tccatctccg gcccgcttcc tacgacaccg
     3061 tgacctggac cgacagcgcg gtccgccggc tcgccctgga agcggaggat gtcctcgagg
     3121 atctgctcga cctcgccgcc gccgatgtca cctccgaacg ggcggccaag cgacgagccg
     3181 ccgccactcg cgtgcaggga ctccgcgatc acatcgcgcg gctggaagaa gaacaggcgc
     3241 tcgaccagct ccagagcccg ctcgacggca acgagttgat gcagatgttc gatcgcaagc
     3301 ccggcaagtg gatcgccgag gtcaagaacc acctccggga actcgtgatc gacggcgagc
     3361 tctcctccga cgacaaggag acggcaacgc ggatcgctcg tgacctgctc gccgcggaac
     3421 cagccccctg atctcccctg cttccgtcgt tacgtgataa tgcacatcgt accgtgaatc
     3481 gaagcccgct gaaggagacc ctatgtcgac catcacctcc accgattcca gcccgcgtga
     3541 cgatgcgaag gaacgtgtca aggcacaata cggctccgtc ggcgatgcct atgtgacgag
     3601 caaggatcac gcgggcggcg atgacctggc acggatggtc gaggtcgcga atccgcaacc
     3661 gaccgaactc ctgatcgata tcgcgaccgg tggcggccac gtcgcgaaga ccttcgcccc
     3721 gcatgtcgcg aaggcgatcg cgacggatct cacgccggaa atcctcctgc atgccggcaa
     3781 cttcttcgcg gaactcggtc tcacaaacgt cgagacgaag ctcgccgacg ccgaggaact
     3841 cccgttcccg gatgcgtccg ccgacatcgt cacctgccgg atcgccccgc accacttccc
     3901 ccatcccgat cgcttcgtac aggaagtgac gcgggtcctg cggcccggcg gacggttcgt
     3961 cctgatcgac agcaccgttc ctgccggtga agccgccctc ttcttcaacc gcttcgagaa
     4021 gttgcgcgac ccgtcccacg tccgctcgct gaccgccgac gagtgggatc tcctgatcac
     4081 ctcgaccgga ctcacgatcg agatcgtcga ggactttccg aaacgccacg atttcgacga
     4141 ctggacggct cgttcccgga tgaccgcgga ggaca
//
LOCUS       DASTMD010000772         3544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2481894, whole genome shotgun sequence.
ACCESSION   DASTMD010000772 DASTMD010000000
VERSION     DASTMD010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2481894"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFQ54_11440"
     CDS             complement(<1..573)
                     /locus_tag="VFQ54_11440"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEU0165647.1"
                     /translation="MSDPTRTGIPDVNDDATWPGVAAAYAAWPGDQAPGSGSAAAPGT
                     IPPPREHLDPKARTIWFINNLIATIVITAVVTAAMVGAHMWFDAALLWTILVPVVVAL
                     ILLTIAWLDPLVRYRIWRFEIHEDEVDLQHGFFTRTRQLVPMSRIQHVDTRQGPLDRR
                     YRLASVIFYTAAGAISIPALSVGRAAEVRSQ"
     gene            744..1514
                     /locus_tag="VFQ54_11445"
     CDS             744..1514
                     /locus_tag="VFQ54_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEU0165648.1"
                     /translation="MAETDTADSTPTGETSPAETKPKRVLLIQAHPDDAEFMCAGTVA
                     RWISEGAEVHYASLTSGDKGSDDPAMTGPELASMREREQRAACDVLGVKSITFLGYPD
                     AMLVPDLAMRLVLTRLMRQIRPDVLLCQDPTMRWGGQGYINHPDHIAAGEASLAAVFP
                     SVRDRLTFPELLNEGLEPHKVNEVYIYGTNSADRWIDITGSIDTKIAALKAHASQIGE
                     WDPTDMMKSWARETANRHPARPDGFGEFAEAFKYVKLD"
     gene            1606..2019
                     /locus_tag="VFQ54_11450"
     CDS             1606..2019
                     /locus_tag="VFQ54_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165649.1"
                     /translation="MVQRKLGMYVANKIKDEIMASPKPGEAIDPATGAVVAAQDGRTT
                     TFVRQDQDDLHGQIVRDADGNAIGRAFRVTDVAVYVIARDGRNPGADLTRAIQTDAAH
                     MHFQTTPNGRITSIIYVADENPSATGLHGARADEV"
     gene            2113..3507
                     /locus_tag="VFQ54_11455"
     CDS             2113..3507
                     /locus_tag="VFQ54_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0165650.1"
                     /translation="MNPTVFASDRGESAPSSPELLDAASRAIAYLSTMGERPVAPSVD
                     AVQRLAGFNYPLPSDPTDPATVIRLLDDLGSPATTAMAGPRFFGFVIGGALPTALAAN
                     WLAGAWDQNATLFESSPIAATLETVTERWLVDLLGLPSGTAASFVTGCTMAHFTALAA
                     ARHAVLKAVGWDVPSQGLFGAPRVTVVVGEEAHASLFKVLALLGFGRDGLVRVPVDDQ
                     GRMRSDALPEVSGPAIVCVQAGNVNTGAFDPIRDVVAWARSIGAWVHVDGAFGLWAAA
                     APELSHLLDGVELADSWASDAHKWLNVPYDSGLAFVRDPLALTDVMTTSAAYLQETEG
                     RREPSWYTPESSRRARGVEIWAALLALGANGVSDIVSRTCGYARRFAMQLEAAGFDIL
                     NDVVLNQVLVSFGDPERTNRVIARVKEDGTCWCGGTVWQGQTAMRISVSSWATTAEDV
                     DISVRAIIAAASAV"
BASE COUNT          624 a         1093 c         1209 g          618 t
ORIGIN      
        1 ctggctgcgg acctcggcgg cgcggcccac ggaaagcgcg gggatgctta tcgcgcccgc
       61 cgccgtgtag aagatgacgc tggcgagcct gtagcggcgg tcgagtgggc cctggcgggt
      121 gtcgacgtgc tggatgcggg acatcggcac cagctggcgg gtgcgggtga agaagccgtg
      181 ctgcagatcg acttcatcct cgtgaatctc gaagcgccag atccggtatc gcaccagtgg
      241 atcgagccag gcgatcgtga ggaggatgag cgcgacgacg accggtacga ggatcgtcca
      301 gaggagcgcg gcatcgaacc acatatgggc gccgaccatc gctgccgtca cgacggcggt
      361 gatgacgatc gtggcgatca ggttgttgat gaaccagatc gttcgagctt tcggatcaag
      421 gtgttcccgt ggtggaggaa tggtgccggg agccgcggcg ctccccgaac ccggtgcctg
      481 gtcgcccggc cacgcggcat aggcggcggc gactccaggc caggtcgcgt catcgttgac
      541 gtccgggatg ccagtgcggg tgggatcaga catgatgccg acggatcctt ctcgtccagc
      601 caggaatggt cgttcctgac cttcgtgtcc ctgccacctt caccgtggca acgtatgatt
      661 catacaacag tacacgcgac tcgaccatcc gcaggagatt cgcgcggata ggtgcgatgc
      721 attcgaagga acaatcaccc aacatggccg agaccgatac cgcagattcg acgccgactg
      781 gcgagacttc cccggctgaa acaaagccaa agcgcgttct tctcatccag gcccatccgg
      841 atgacgccga gttcatgtgc gccggcaccg tcgcgcgctg gatcagcgag ggcgcggaag
      901 tccattacgc ctcgcttacc agcggtgaca aggggagtga cgaccccgcg atgaccggcc
      961 cggaactcgc ctccatgcgg gagcgggaac agcgcgcggc ctgcgacgtg cttggcgtaa
     1021 agtccatcac cttcctcggc tatcccgacg cgatgctcgt acccgatctc gcgatgcgcc
     1081 tcgtgctgac gcgactgatg cgccagatcc gaccggacgt cctgctctgc caggatccga
     1141 cgatgcgctg gggtggacaa ggctatatca accatcccga ccatatcgcg gcgggcgagg
     1201 cgtcgctggc ggcggtcttt ccgtcggtgc gggatcgcct gaccttcccc gagcttctga
     1261 acgagggcct cgagccgcac aaggtgaacg aggtctatat ctacgggacg aattccgccg
     1321 atcgctggat cgatatcacc ggcagcatcg acacgaagat cgcggcgctc aaggcgcacg
     1381 cgagtcagat cggcgaatgg gatccgacgg atatgatgaa gagctgggca cgggaaacgg
     1441 cgaatcgaca cccggcacga cccgacgggt tcggcgagtt tgcggaagcc ttcaagtacg
     1501 tgaaactcga ctaggtgcgg ctccggtcgc tttcggcgtc attcgcagcc aacaatctga
     1561 agacgccgat cgatggattt cgacagacga tagagaggca aggacatggt tcagcgcaaa
     1621 ctcggtatgt atgtggcaaa caagatcaag gacgagatca tggcgtcccc gaaaccgggc
     1681 gaggcgatcg accccgcgac cggtgctgtc gtggcggcgc aggacggccg gacgacgacg
     1741 tttgtccgtc aggaccagga cgatctccac ggccagatcg tgcgtgatgc cgatggcaac
     1801 gcgatcggtc gggcgttccg ggtgacggat gtcgcggtct acgtgatcgc gcgggatggc
     1861 cgcaacccgg gcgcggacct gacgcgggcg atccagacgg atgccgccca catgcatttc
     1921 cagacgacgc cgaacggtcg catcacctcg atcatttacg tcgccgacga gaacccttct
     1981 gcgaccggac tccacggcgc ccgcgccgac gaggtctagg cgcgggcgcc gtgggtcgtt
     2041 cagcatctcg atcggccatc ggccgtccga gcggtctcgt ggcgatattg ggtcatttct
     2101 ggaggcagcg tgatgaaccc cacggtcttc gcatctgaca ggggggaatc cgctccctct
     2161 tcgcccgagt tgctggatgc ggcctcgcgc gcaatcgcct acctctccac aatgggcgaa
     2221 cgcccggtgg cacccagcgt cgacgcggtg cagcgcctgg ccggattcaa ttacccgctg
     2281 ccgtcggatc cgaccgatcc cgcgacggtg atccggttgc tcgacgacct tggttctcca
     2341 gcgacgacgg caatggcagg accgagattc ttcggattcg tcatcggggg cgccttgcca
     2401 accgcgcttg ccgccaactg gttggccggg gcgtgggatc agaatgcgac gctcttcgaa
     2461 tcgtccccga tcgcggcaac ccttgagacg gtaaccgagc gctggctcgt cgatctgctt
     2521 ggcctgccat ccggaaccgc cgcgtcattc gtcacggggt gtacgatggc gcacttcacg
     2581 gccctcgccg cggcccgcca cgcggtgctg aaggcggtgg ggtgggatgt cccgtcgcag
     2641 ggactctttg gcgcgccgcg ggttaccgtg gtcgttggcg aggaggcaca cgccagcctc
     2701 ttcaaggtgc tcgcgctgct cggcttcggg cgagacgggc tggtgcgggt tcccgttgac
     2761 gatcagggcc ggatgcggag cgacgcactt ccagaggtga gcggtccggc gatcgtgtgc
     2821 gttcaggccg gaaacgtcaa caccggtgca ttcgatccca tccgggatgt cgttgcgtgg
     2881 gcccgaagca tcggagcctg ggtccatgtg gatggcgcgt tcggattgtg ggcggccgcg
     2941 gcgccggagc tgtcgcatct cctggacggg gtggaactcg cggattcgtg ggccagtgac
     3001 gctcacaagt ggctcaacgt gccctatgac agcgggctgg ctttcgtgcg cgatcctctc
     3061 gcgttgacgg acgtgatgac gacctcggcc gcctacctgc aggagacaga gggtcgtcgc
     3121 gaacccagct ggtacacgcc ggaatcgtcg cgtcgcgcgc gaggggtcga gatctgggcg
     3181 gcactgctcg cgctcggcgc gaacggcgtc tccgacatcg tttcccgaac ctgtggctac
     3241 gcccggcggt ttgcgatgca actcgaggcg gccggtttcg acatcctcaa cgatgtcgtc
     3301 ctcaaccagg tcctggtctc gttcggcgac ccggagcgca ccaaccgggt gattgcgagg
     3361 gtcaaggagg atggcacgtg ctggtgtggt ggaacggtct ggcaagggca gaccgcgatg
     3421 cggatcagcg tttcttcctg ggccactacc gcggaggatg tggacatctc cgtacgggca
     3481 atcatcgcgg ccgcctcggc tgtctgaggg gcggatctga cggcgaaggg gtgaggaccg
     3541 acag
//
LOCUS       DASTMD010000773         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2572739, whole genome shotgun sequence.
ACCESSION   DASTMD010000773 DASTMD010000000
VERSION     DASTMD010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572739"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..724
                     /locus_tag="VFQ54_11460"
     CDS             176..724
                     /locus_tag="VFQ54_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165651.1"
                     /translation="MKRFGTSVLVSLVLLGGVMGQAVAADDATPTPLAAAGYPVAIHE
                     GTCASPTAQPAYEIDNTTIVGSGSDPSDTIGGTVNQPVLEASATVKAKLDDVASQPHV
                     IAVHASPTDYNTLIACGQVAGTKEDGKLVVALIPVNGSGVSGVAILDEDTSGVLGLGD
                     DQIKATVYLIAPSQPGEATPSS"
     gene            873..1145
                     /locus_tag="VFQ54_11465"
     CDS             873..1145
                     /locus_tag="VFQ54_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165652.1"
                     /translation="MIAFFWLEEYAHREDGWGLWPRAHEQTTVSAARSWLDTQWLGTH
                     AGSSFLTLQLPDGTRMTKTAVDLDKVLASWDESCALTVVGPIGDDA"
     gene            1243..1671
                     /locus_tag="VFQ54_11470"
     CDS             1243..1671
                     /locus_tag="VFQ54_11470"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0165653.1"
                     /translation="MSTGTGKKDRRGRVNHLGFGDRQWLGRLLRDRRLAQRRSLADVA
                     NVAGIDPGHLSRVERGLSVPGEDVVSCVAAALHLDLDELSAAEEEIIARYRLLETWLD
                     RFAIADASRKDLMSVAPATCADLISVFESLATSATQDGRP"
BASE COUNT          304 a          524 c          569 g          312 t
ORIGIN      
        1 caccggcccc cttgccgacg gcgcacgtga ttacgcagaa aacatgccag cgtttgcgtc
       61 cagttgtcgc aaacatcgga cgcgtgccat gccaggcgaa ctggccccgt tcctgcgtct
      121 gctcccggta cgcgggaaca ctgtgggttt ccgatggaga tagatggagg gtcctatgaa
      181 gcgattcggt acaagcgtcc tggtgagtct tgtcctgctg ggtggcgtga tgggccaggc
      241 ggtcgcggcc gacgatgcaa cgcccacacc gcttgcggcg gccggttatc ccgtcgccat
      301 ccatgaagga acgtgtgcga gtccgacggc tcagcccgcc tatgagatcg acaacaccac
      361 catcgtcggg tcgggcagtg atccctccga cacgatcggg ggaaccgtca accagccggt
      421 gctcgaagcg tccgccacgg tcaaggcgaa gctcgatgat gtggcgagcc aaccccacgt
      481 catcgccgtg catgcgagtc cgacggacta caacacgctg atcgcctgcg ggcaggttgc
      541 gggaacgaag gaggacggga agctcgtggt ggcgctcatc ccggtgaacg gctccggcgt
      601 gagtggcgtg gcgattctcg atgaggatac gtccggcgtc ctcggtcttg gtgatgatca
      661 gatcaaggcg acggtctatc tgattgctcc gtcgcagccg ggtgaggcga ccccgtcttc
      721 gtaaagcagc ggatcacctg cgacaagccc ccacagtgac aacgcactgt gggggccgct
      781 gcgttgtgcg atgctagttc ctacgggctc cggcgtctcc gggtcgtcat cccgccgcca
      841 agaccggtga tcgccaggag caagcgtcca gcatgatcgc atttttctgg ctggaagaat
      901 atgcccatcg cgaagacggg tgggggcttt ggccacgcgc gcacgagcaa acgaccgtgt
      961 ccgccgcccg ttcctggctt gacacgcagt ggctcggcac gcatgcggga tcgtcgtttc
     1021 tgacgctgca gcttccggac gggacgagga tgacgaagac cgcggtggat ctcgacaagg
     1081 tattggcgag ttgggacgaa tcatgcgccc tcacggtggt ggggccgata ggcgacgatg
     1141 cttgaccgac gaccccgacg acgaatcacc tgagcgaaac gacgattggc cccggctcgc
     1201 gttcagcggt gtggtccatg tgagatggtg gaggagtgca gcgtgagtac aggtaccggc
     1261 aagaaggaca ggcgcgggcg cgtcaaccat ctcgggttcg gggatcggca gtggctcgga
     1321 cggctccttc gtgatcggcg gctcgctcag cggcgttccc tcgctgatgt cgccaacgtc
     1381 gcgggaatcg atccgggcca tctgagccga gtcgaacgcg gtctgtccgt gcccggggag
     1441 gacgtcgtct catgcgtggc cgccgcgctc cacctcgacc tggacgaact cagtgctgct
     1501 gaagaggaga tcattgcgcg gtatcgcctc ctcgaaacct ggctcgaccg attcgccatc
     1561 gcggacgcgt cgcgcaagga tctcatgagt gtcgctccgg cgacctgcgc cgatctgatc
     1621 tccgttttcg agtcacttgc cacgtctgcc acgcaggatg gtcgtccgtg atcgcgtcag
     1681 gtatggcagg ctctcacacg agccgaacc
//
LOCUS       DASTMD010000774         4395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_339518, whole genome shotgun sequence.
ACCESSION   DASTMD010000774 DASTMD010000000
VERSION     DASTMD010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339518"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFQ54_11475"
     CDS             complement(<1..413)
                     /locus_tag="VFQ54_11475"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0165654.1"
                     /translation="MTEHQPATDEPTIQFETKAVPEQEDAAAAIILAPATGAGTAKAA
                     LAKIIELRAEEKAGFYGGYINGELIGAYGMRRDGMANTIAIIAVREDFRRKGIGRALL
                     TDALYRSARRPLTAETDDEGLAFYKACGFKLVGRRV"
     gene            complement(440..1852)
                     /locus_tag="VFQ54_11480"
     CDS             complement(440..1852)
                     /locus_tag="VFQ54_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0165655.1"
                     /translation="MVHDRSHSSFRRSASGFDASLPGFNPGLIRAATATPSTSPGGFD
                     TPLYLKSTLANGVRVVTGPMTGVRSASLIFYYNIGSRFEPAPIAGVSHFLEHMLFKGT
                     ERRPDPMTISEEIEGVGGIVNAGTGRESTSYWCKVPSTHVELAFDVMADMLRNSLFLE
                     EELTKERKVIVEEIRSTQDVPEDLVHDVIDEVVWGTDPIGREIAGSEETVHNITREVM
                     VDFFHRNYTPDRLVIAAGGDVRHEDIVALTEKYFGDLAAVGEKDMYERVAVTQDARRI
                     KVLNRETEQAHLCVSFPAVSYYDDRRYAQSTIEAILSSGMSSRLFQEIREKRGLVYSV
                     YGYFRGYEDIGQGVIYAGTDLERVDETIEAIVGELRKLRDVPVSAEELEHTKTLRKGR
                     LLMGLEDSRSVASWIGSQEATYGEIKTPEEVMEKIYAVTIEDVQSLATEIIRDELLSL
                     ALIGPYDDATHFDPLLRLGN"
     gene            2008..2652
                     /locus_tag="VFQ54_11485"
     CDS             2008..2652
                     /locus_tag="VFQ54_11485"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU0165656.1"
                     /translation="MEHFEIGDTMIMRGRIGPRIGLVIPATLVFDEPDAFGLYVQPGT
                     PCKRLVPPNGVSLPRVLRPDQIVDPALQVVDSVWSETHLLHVTPTGREHSIYVRWGED
                     WSFRGWYVNMQAPLQRFGRIVETEDLYLDIVIEHDFSWQWKDEDELAEAVAVGRISTV
                     TAQAVRAEGERVLTEMSARLWPFVPHISSWRPDPAWTTPELPEDWDAPMAPAPI"
     gene            complement(2649..2960)
                     /locus_tag="VFQ54_11490"
     CDS             complement(2649..2960)
                     /locus_tag="VFQ54_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165657.1"
                     /translation="MQRVAEWFRGWLRRHPVLAVVWVLFLALCIFSTFAGEEGDRPSP
                     VYGVVVLPILGALVILYFSRTVERFQKIRALSGQRAAPQHVHDHDHGDDHDHDHDHDH
                     H"
     gene            complement(2983..4041)
                     /locus_tag="VFQ54_11495"
     CDS             complement(2983..4041)
                     /locus_tag="VFQ54_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU0165658.1"
                     /translation="MSLFRIYVSADEDYLAIATTELREVFPDAVFRPLGPDFFSMEGD
                     GVSIANVADACRDRANPLAFPRHLMRGVGEFPLPTTIDELGEVIEGALAAWQELPLPA
                     SLSLQVWGSGSEPPALPVRMDEFWRLLAEGLRDEEIEVGRAGHDHVLSVCVTQGGIIV
                     GSNRRDSALSDWPGGRVRLAKPKGQISRSEFKLEELLRSNEVTLPEGVALDLGASPGG
                     WTRILRERGLTVWAIDPGDLDPRVVADRGVHHVRTTAGSFFHNSDRMFDLIVNDMRMT
                     PALSTELMLTASGHLNPSGMIIQTLKISPHHGLEMVRNALSTLRRRYEIDLVRQLHHN
                     RNEVTVVAHGLNSMSIAG"
BASE COUNT          815 a         1391 c         1420 g          769 t
ORIGIN      
        1 acgcggcgtc cgaccagctt gaacccgcac gccttgtaga aggcgagtcc ctcgtcatcc
       61 gtctcggcgg tgagcggtct cctggcggag cgatagagcg cgtcggtcag gagtgcgcgc
      121 ccgatgcctt tccgccgaaa gtcctcgcgt acggcaatga tcgcgatcgt gttcgccatg
      181 ccgtcgcgcc gcatgccata cgcgccgatc agctcgccat tgatgtatcc gccgtagaaa
      241 ccagcctttt cctcggcgcg aagctcgatg atcttcgcga gggcggcctt cgccgtcccg
      301 gcgccagtcg ccggcgcgag gatgatcgcc gcggcggcgt cctcctgctc gggaaccgcc
      361 ttcgtctcga actggatcgt gggctcgtca gtggcgggct gatgctcggt catgattgct
      421 ctcgaatctc cgccagacgc tagttaccga gtcgaaggag cgggtcgaaa tgagtcgcgt
      481 cgtcatacgg gccgatcagc gccaggctga gcagctcgtc gcggatgatc tcggtcgcca
      541 gggactggac gtcctcgatc gtgacggcgt agatcttctc catcacctct tccggcgtct
      601 tgatctcgcc gtaggtcgct tcctggctgc cgatccacga ggcaaccgaa cggctgtcct
      661 cgagacccat cagcaggcgt cccttgcgca aagtcttcgt gtgctcgagc tcttccgccg
      721 aaacggggac gtcgcggagc ttccgcagct cgccgacgat cgcctcgatc gtctcgtcga
      781 cacgctcgag atcggtgccg gcgtagatca cgccctgccc gatatcctcg tagccccgga
      841 agtagccgta gacggagtag acgagaccgc gcttctcccg gatctcctgg aagaggcggg
      901 aactcatccc cgacgacaga attgcctcga tcgtcgactg cgcgtagcga cggtcgtcgt
      961 agtacgagac ggccgggaag gagacgcaga gatgcgcctg ctcggtctcc cggttcagga
     1021 ccttgatccg gcgcgcatcc tgggtgacgg cgacgcgctc gtacatgtcc ttctcaccga
     1081 ccgcggcaag atcgccgaag tacttctcgg tgagggcgac gatgtcctca tggcgaacgt
     1141 cgccaccggc ggcgattacg aggcgatccg gcgtgtagtt acgatggaag aaatcgacca
     1201 tcacctcacg cgtgatgttg tggaccgttt cctcgctacc ggcgatctcg cgcccgatcg
     1261 gatccgtccc ccagacgacc tcgtcgatca cgtcgtgaac gagatcttcc ggcacatcct
     1321 gggtcgagcg aatctcctcg acgatgacct tgcgctcctt cgtcagttcc tcctcgagga
     1381 agagcgaatt gcggagcatg tcagccatca cgtcgaacgc cagctcgacg tgcgtgctgg
     1441 ggaccttgca ccagtaggag gtcgattcgc gtccggtccc ggcgttgacg ataccgccga
     1501 cgccttcgat ctcctcggag atcgtcatcg gatccggtcg tcgctcggtg cccttgaaga
     1561 gcatgtgctc gaggaagtgg gagacaccgg cgatcggcgc aggctcgaac cgcgagccga
     1621 tgttgtagta gaagatgagg ctcgcggagc ggacacccgt catcgggccg gtgaccacgc
     1681 gaacgccatt cgcaagcgtg ctcttgaggt agagcggtgt gtcgaacccg cccggagacg
     1741 tcgatggagt cgcagtcgcg gcgcggatca gacccggatt gaagccaggc agggacgcat
     1801 cgaagccgga tgcggaacgg cggaaagagc tatgagaacg atcgtgtacc aagggaactc
     1861 cttctcctgc ctcaaactgg aacgaggcgg tgagatggaa cagtaagaca aggcaaggtg
     1921 atgggccagt aaccaccact ggatgtatgg gcagggtacc gcgaatcgca ggcaatggca
     1981 cagcggacca cgacgaaagg agtcagcgtg gagcacttcg aaatcggcga caccatgatc
     2041 atgcgcggtc gcatcgggcc gcggatcggg ctcgtgatcc cggccacgct tgtcttcgac
     2101 gagccggacg cgttcgggct ctacgtccag ccgggaacgc cctgcaagcg gctcgtgccg
     2161 ccgaacggcg tctccctccc acgcgtcctc cggccggatc agatcgtcga tcccgccctc
     2221 caggtggtcg attccgtctg gtcggagacg cacctgctgc acgtcacacc cacgggccgc
     2281 gagcattcga tctacgtgcg ctggggtgag gattggtcct tccgcggctg gtatgtgaac
     2341 atgcaggccc cgctccagcg cttcggccgg atcgttgaga ccgaggatct ctacctcgat
     2401 atcgtgatcg agcacgattt ctcgtggcag tggaaagatg aggacgagct ggcggaagcc
     2461 gttgcggtcg gacgcatcag caccgtgacg gcgcaggcgg tccgcgccga gggggaacgc
     2521 gtcctcacgg aaatgagtgc ccggctctgg ccgttcgttc cgcacatctc atcctggcga
     2581 ccagatcccg cctggaccac gccggaactg cccgaggatt gggatgcgcc catggcgccg
     2641 gccccgatct agtggtggtc gtggtcatga tcatggtcgt ggtcatcacc atgatcgtgg
     2701 tcgtgaacat gctggggcgc ggcgcgttgg cccgagagcg cgcggatctt ctggaagcgt
     2761 tccacggtgc gcgagaagta gaggatcacc agcgcgccga ggatcggcag gaccacgaca
     2821 ccatagactg gactcggccg gtcaccttcc tctcccgcga acgtcgaaaa aatgcagagg
     2881 gcgaggaaga gcacccagac cacggccagc acgggatggc ggcgaagcca gccgcgaaac
     2941 cactcggcga ctctctgcat gtcaggttcc tttctctccg gatcagccgg cgatcgacat
     3001 cgaattgaga ccgtgggcga cgaccgtcac ttcattccga ttgtgatgca gttgacggac
     3061 gaggtcgatt tcgtaacgtc gccgaagcgt cgaaagcgcg ttccggacca tctcgagccc
     3121 gtgatgcggc gagatcttca gggtctggat gatcatcccg ctcggattca gatggccgga
     3181 tgccgtcagc atcagttccg tcgagagcgc gggcgtcatc cgcatgtcgt tcacgatcag
     3241 gtcgaacatg cgatcggagt tatggaagaa ggagccggcc gtggtgcgca cgtgatgcac
     3301 gccacgatcc gccacaacgc gcgggtcgag atcgcccgga tcgatcgccc agacggtcaa
     3361 gccacgctcg cgcaggattc gcgtccagcc gcccgggctg gcgccgagat cgagcgccac
     3421 gccttctggc agcgtgacct cgttcgagcg cagcaactcc tccagcttga actcggagcg
     3481 tgagatctgt cccttcggct tcgcgagacg aacgcgcccg cctggccagt cggaaagcgc
     3541 gctgtccctg cggttcgaac caacgatgat cccgccttgc gtcacgcaga cggagagcac
     3601 gtggtcgtgg cccgcccgcc cgacctcgat ctcctcgtca cggagccctt ctgccagcag
     3661 gcgccagaac tcgtccatcc ggacgggcag ggccggcggc tcgctgccgc ttccccagac
     3721 ctggaggctg agcgatgccg gcagcgggag ttcctgccac gccgccagtg caccctcgat
     3781 cacctccccg agttcgtcga tcgtcgtcgg caggggaaac tcgccgacgc cacgcatcag
     3841 gtgacgtgga aaggcgagcg gattcgcgcg gtcgcggcag gcgtccgcca cgttcgcgat
     3901 gctgacgcca tcgccttcca tcgaaaagaa atcgggaccg agcggtcgga agacggcgtc
     3961 aggaaagacc tcgcgcaact cggtcgtggc gatcgcgagg taatcctcat cggcagaaac
     4021 ataaatccgg aaaagcgaca cgaaggggga gctcctggga tggattcggg gatgccaagc
     4081 tggccttgtt gtgacaagca tcgcatatgc gcaggtttgg ggttgccaag gggccttcgg
     4141 catgagattc gaaggaaaga gagccgcgag cggcaacacg agcagtggct cgccgtcgac
     4201 gatcctgctt ccgacgatgc ggtcgatcga gtgtcatcgg atcgacacgg tctgaagcgc
     4261 aggagaccgg cgcccggagc ggacgccggt ctcgtcaggt tgctcggcgc tcgcccaggg
     4321 agatcaggat tcgatcttga ctggacccga accgtgactc ccgaagatcg cctgcacgaa
     4381 ggcggcgaca tcccc
//
LOCUS       DASTMD010000775         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2035788, whole genome shotgun sequence.
ACCESSION   DASTMD010000775 DASTMD010000000
VERSION     DASTMD010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035788"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFQ54_11500"
     CDS             <1..218
                     /locus_tag="VFQ54_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165659.1"
                     /translation="EATQVLENLGKERFNFLLRQLQATLQEAARDSDAETVSLVKDQL
                     TVLAERHRQYYPAPSPYFHDSRTQQQH"
     gene            complement(733..1158)
                     /locus_tag="VFQ54_11505"
     CDS             complement(733..1158)
                     /locus_tag="VFQ54_11505"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0165660.1"
                     /translation="MTDKLSTTEPIDRTQLPTDQFGWGFTKWLVTPDQPGGAGMSFGE
                     VIALPGMGHDRHNHPDAEEILYFLSGTGLQMVDDEEPFEVHAGDVVYIPKAIFHSTLN
                     TGWEPLKILAIYNPGGSERALRELPDYRLVPPGGAPKFG"
     gene            complement(1189..2025)
                     /locus_tag="VFQ54_11510"
     CDS             complement(1189..2025)
                     /locus_tag="VFQ54_11510"
                     /inference="COORDINATES: protein motif:HMM:NF020927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HEU0165661.1"
                     /translation="MAFTRQEVLDRLHAQIEAGRPILGAGAGTGLSAKCLEAGGADLM
                     VIYNSGRFRMAGRGSLAGMMPYGDANAIVVEMAGEVLPVVKETPVLAGVCGTDPFRLM
                     PVFLKQIKEMGFSGVQNFPTVCLYDGKFRANLEETGMGYGLEVEMIREANRLDLFTSP
                     YAATPEEAAAMAEAGADIVVAHMGLTTSGLIGAETAMSLDEAATRVAAIYAAAKGARE
                     DVIVICHGGPIASPDDAQYVLDRVPGLAGFYGASSIERLATEVALIEATRKFKDTHFA
                     SA"
     gene            complement(2065..>2565)
                     /locus_tag="VFQ54_11515"
     CDS             complement(2065..>2565)
                     /locus_tag="VFQ54_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HEU0165662.1"
                     /translation="VLDLTTTEFADLYVGATLPAGPERLLSAGRAGYPLVVSVGALDM
                     ANFGPIETVPERFRERNLHRHNAAITLMRTTAEESRAIAGVIAERLNQATGPVTVFFP
                     LRGVSMIDIAGQPFHDPEADAVLFATLRKRLDPRIKVVELDIDINDPTFAIAMADELH
                     RLYEST"
BASE COUNT          469 a          843 c          787 g          466 t
ORIGIN      
        1 gcgaggcgac gcaggtcctg gagaatctcg ggaaggagcg gttcaacttc ctcctgcggc
       61 aactccaggc gaccctccag gaagccgcac gcgacagcga cgccgagacg gtctcccttg
      121 tgaaggatca actcacggtt ctcgccgagc gccatcgcca gtactatcct gccccgagcc
      181 cctactttca cgatagtcgc acgcaacaac agcactagac aatccgggac caccacaaga
      241 caatagtgat atacgcgcgt acgtacgcgg gtatatcact attttccatt cctgaatggt
      301 ttcgccgtca cgccgcgacg gactcgaatg catgatgccg cgtcgtccaa atacccctgg
      361 cggcgagcag ttcgtctcct acgctgtcgc atggaccgag ctgagcggtc gcaggctcga
      421 gccgcatctt cgtccgcgga ttgcttttcg cccgagctcc ctcctgcaat cgggccgttg
      481 gaggaaccgt agacgcggcc cttgtgaccg caccgcatcg gcttcgccga tccgtcctcc
      541 caaacgagtt atggattgcg tgttcccgtt ggagtccgcc tatggcggat gtgcttcgca
      601 cggggatttc tcgacaagct cgaaatgaca attccatggg acggaaatgt gcgggaagcg
      661 cgcggacgcc gacaggtccc tcgacaagct cgggaggacg tgtcgtgtga ggcgtcgcga
      721 gggacaggta gcctagccga atttcggagc gccgccgggc ggaaccaggc gatagtccgg
      781 caactcccgg agtgcgcgct ccgaaccgcc aggattgtag atcgcgagga tcttcagcgg
      841 ttcccagccg gtattcagcg tcgagtggaa aatggctttc gggatgtaga cgacgtcgcc
      901 cgcgtgaacc tcgaatggct cctcgtcatc caccatctgg agaccggttc ccgacaggaa
      961 gtagaggatc tcctcggcgt ccggatggtt gtggcgatcg tgccccatgc ctggcagcgc
     1021 gatcacctcg ccgaagctca tccccgcgcc acccggctgg tccggtgtca ccagccactt
     1081 cgtgaagccc cagccgaatt gatcggtcgg gagttgcgtg cggtcgatcg gttcggtcgt
     1141 cgagagcttg tcggtcatcg gaatcctcct cggggcgaga tcacgccgtc aggcgctggc
     1201 gaaatgagtg tccttgaact tccgcgtggc ctcgatcagg gcgacttccg tcgccagccg
     1261 ctcgatgctc gacgcgccgt agaagccagc cagcccgggc acgcgatcga gcacgtactg
     1321 ggcatcgtcc ggcgacgcga tcgggccacc gtgacagatt acgatcacat cctcccgcgc
     1381 gcccttcgcg gcagcgtaga tcgccgccac ccgcgttgcc gcctcatcca gcgacatcgc
     1441 cgtttcggcg ccgatcagac cggaagtggt caaacccatg tgggcgacca cgatgtctgc
     1501 accggcttcc gccatcgctg cggcttcttc aggagtcgca gcgtacgggg aggtgaagag
     1561 atcgaggcga ttcgcttcgc ggatcatctc gacctcaagg ccgtatccca tccccgtctc
     1621 ctcgagattg gcgcggaact tgccgtcgta gaggcaaacc gtcgggaagt tctggacacc
     1681 ggagaacccc atctccttga tctgcttgag gaagaccggc atcaggcgga agggatcggt
     1741 cccgcagaca ccagcgagca ccggtgtctc cttcacgacc ggcaggacct cgcccgccat
     1801 ctcgacgacg atcgcgttcg cgtcgccgta cggcatcatg ccggcgagcg agccacgacc
     1861 ggccatccgg aagcggccgg agttgtagat caccatcagg tcggcgccac cggcctccag
     1921 gcacttcgcg gagagcccgg tccccgctcc ggccccgagg atcggccttc ctgcttcgat
     1981 ctgcgcatga agacggtcca gaacttcctg ccgcgtgaac gccatcgtgg aaatctcctt
     2041 gagcttgccg ctcgtccggt gcgattacgt gctttcgtaa agccggtgta gttcatctgc
     2101 catcgcgatc gcgaacgtcg ggtcgttgat gtcgatatcc agctcgacta ccttgatccg
     2161 cggatcgagt cgtttgcgga gggtcgcgaa caggaccgcg tccgcttcgg gatcgtggaa
     2221 cggctgcccc gcgatgtcaa tcatcgagac gccgcggagt gggaaaaaga cggtgaccgg
     2281 acctgtcgcc tgattgagac gctcggcgat gaccccggca atcgcccggc tctcctcggc
     2341 ggtcgtccgc atcagcgtga tcgcggcatt gtggcgatgg agattccgct cgcgaaaccg
     2401 ctcgggcacc gtttcgatcg gcccgaaatt ggccatgtcc agcgcgccaa ccgaaacgac
     2461 gagcggatat cccgcacgcc ccgcactcag caggcgctcc ggaccggccg gcagcgtcgc
     2521 gccgacgtac agatcggcga actcggtggt ggtcagatcg agcac
//
LOCUS       DASTMD010000776         4773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_695712, whole genome shotgun sequence.
ACCESSION   DASTMD010000776 DASTMD010000000
VERSION     DASTMD010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695712"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VFQ54_11520"
     CDS             <1..859
                     /locus_tag="VFQ54_11520"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU0165663.1"
                     /translation="GTDCPPIVVRTTGFAGGHAVVPGDLSSQFFTGLLLAAPYARDGV
                     TIEVEGDLVSKPYITITADVMRAFGVETEIDDVNWKSFRVAPGQRYVGRDYHIEPDAS
                     NASYFFAAAAVTGGEIRVDGLGTTSTQGDLQFVRVLEQMGAAVTMTDSATTVRGPANG
                     ALRGGDFDFNVISDTAQTLAAIAPFADSPVTIRGIAHNRVKETDRIGDLAIELRRLSQ
                     AVDEFEDGLTIHPRPVVAAEIHTYDDHRMAMSFAVTGLRTPGVTILDPGCTAKTFPDF
                     FDRLAELTS"
     regulatory      978..1085
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1418..3136
                     /locus_tag="VFQ54_11525"
     CDS             1418..3136
                     /locus_tag="VFQ54_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0165664.1"
                     /translation="MPGFEKLSSRVSRRTILKTSAAGAAVSLLAAGGTVAAEAAGSRA
                     DAPALSPQDAGTPTPTPPTPKPGGTIIYNIAQEGSHLVPPFSSFSTVIEPTVPFFNGL
                     TRPGVDREPTPDLAESWTANTDGTHYVFKLRQGVTWHDGQPFTAADVKFTWELIANPK
                     NTTAAQLYSFFSKIVGADDFHTGKATEITGIKAADDLTLDVTLASPWAPFLTIASNQY
                     IVPKHILGDIAVDKILEDGYARAPIGTGPFTFKAWQAGDSIIGEAFDNYYGGRPYADT
                     IVMRVAGLDANGELSALDSGELNESDLTLVQADTLTGDSAVVLNERAGEANQYVEFNL
                     AKPLFQDVNVRKALSYALDRNAISQAVWQGRAQIYNSVFPYDWWCTKKDTTIFDNDAD
                     QAKKLLDAAGWTVGSDGIREKDGTKFSFTAYSISPDWSLVVQQQWKAIGIDVKLQMVD
                     FPKLSTQYYTTHLFDVVFLNIPYTLYTDPHYSLPGYFLSANNRNAYKNPKSDELIIAA
                     ASTNDQEQRKQLYYQWQELIAQDVPHLWIGNPTAPTAYSAGLYYPDRSSSYFSWREIR
                     DWYWTE"
     gene            3177..4100
                     /locus_tag="VFQ54_11530"
     CDS             3177..4100
                     /locus_tag="VFQ54_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165665.1"
                     /translation="MGSAPEATSPGGAVTPLGRGERTAAPETGQARRILVAWLRNPRL
                     AVGGLVIAVVCLLAIFAPWVTQTAPDVQNPLYRLAKPGNHFLLGGDSFGRDIYSRLVY
                     AGRVSLSVGVLSMVITVVVGVLVGIVAGYYGGWIDRLLMRLTDIVLVFPTFFLLVFVV
                     ATFGSSVKLLIVMIGLTSWPTNARVVRAAVLRLRDHEFIVAAKVNGARGSWIMQKHLL
                     PQLLPIVIASATIRVANNILIESGLSYLGLGVQPPTPTWGNMISDSAVYMRQAWWLMA
                     IPAITIFVVVLAFNLLGEGLRDQLDPRRRGR"
     gene            4104..>4773
                     /locus_tag="VFQ54_11535"
     CDS             4104..>4773
                     /locus_tag="VFQ54_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165666.1"
                     /translation="MRYWLNRILEIVPLLIGISLLTFVIFQLTPGDPIGLVVDPTLLS
                     ADERSAVRQSLGLDDPLPIQYVKMMEGIVDGNLRSFKTKQPTIDMVWTAFPITLVIGA
                     GGLILALLIGLPVGSLAARRPGGIADKIVSLTLSTSLATPQFALGLILVLIFTVKLHL
                     LPGSGIAPIGTVGFDGVHSIRYLIMPVAVIAFGQVAIFARYVRDALVDVLANEYIRTA
                     RAKGL"
BASE COUNT          862 a         1630 c         1388 g          893 t
ORIGIN      
        1 cgggacggac tgcccaccga tcgtcgtccg cacgacgggg ttcgccggcg gccatgcggt
       61 cgttcccggc gatctcagca gccagttctt caccggcctc cttctcgccg caccgtacgc
      121 gcgcgacggc gtcacgatcg aggtcgaggg cgatctcgtc tccaagccct acatcacgat
      181 cacggccgat gtcatgcgcg ccttcggcgt cgagaccgag atcgacgacg tgaactggaa
      241 gagcttccgc gtcgcgcccg gtcagcgtta cgttggccgc gactatcaca tcgagccgga
      301 cgcctcgaac gcctcctact tcttcgcggc ggcggcggtc accggcggcg agattcgcgt
      361 cgacggtctc ggtacgacgt ccacacaggg cgatctccag ttcgtccgcg tcctcgagca
      421 gatgggtgcc gccgtcacga tgaccgacag tgcgacgacc gttcgcggac ccgcgaatgg
      481 cgcgctgcgc ggcggggact tcgatttcaa tgtgatctcg gataccgcgc agacgctggc
      541 ggcgatcgct cccttcgcgg attcgccggt cacgatccgc ggcatcgccc acaaccgcgt
      601 caaggagacc gatcgcatcg gcgatctcgc gatcgagctc cgccgcctca gccaggcggt
      661 cgacgagttc gaggacggcc tgacgatcca cccccgaccc gtcgtcgcgg ccgagatcca
      721 cacctacgac gaccatcgca tggcgatgag cttcgcggtg accgggctgc ggacgcccgg
      781 cgtcacgatt ctcgatcccg gctgcacggc gaagaccttc ccggatttct tcgaccgcct
      841 ggccgaactc accagctagc cgccttcgcg acaatggcaa gcacatcaag ttacgtattt
      901 cacgatcctg agcagcgggg tattgacacg tatacatccg tcacatagag tcgtctgcaa
      961 caatcgaata gttcacgacc tcatccagag gggcagaggg atcggccctg tgaagcccca
     1021 gcaacccccc gacatcgttc gtcgggaagg tgctaattcc gacaggcatt cctggaagat
     1081 gaggggcgtg agtaaacgtc cttctctgtg ttggccgtgt ccactcgcct ctcatcccaa
     1141 gatgaggggc attttgttgc accacggatc tcacaccggc tcgtccatcc gacccggcat
     1201 catctccttt cgcttgtacg gaccagcgac gcattacatg ggcaaggtcc catgtagcgg
     1261 ctgtagcgat cgctgttgat ccatatgggc tgaattcgcg ggtatcgcgg atgtcccggg
     1321 gcgtagcccc tccgacacca cagggtcatt gtctttgcaa ttccgtatcc ccatacggtc
     1381 aatttgtgca ccttgaacag ggcgcgagga gttttgcatg cctggtttcg agaaactctc
     1441 gtcaagagtc tcccgtcgaa cgattctgaa gacctcggcg gccggcgccg ccgtttcgct
     1501 cctcgccgcc ggaggaaccg tcgccgccga ggcagcgggt tcccgggcgg acgcccccgc
     1561 gctcagcccc caggatgccg ggacgccaac tcccacccca ccgacaccga agccgggtgg
     1621 gaccatcatc tacaacatcg cgcaggaagg ttcccacctc gttcctccct tcagttcctt
     1681 ctccacggtg atcgaaccga cggtcccctt cttcaacggc ctcactcgtc ccggcgtcga
     1741 ccgcgaaccg acgcccgacc tcgcggagtc gtggaccgcg aacaccgacg gcacccacta
     1801 cgtcttcaag ctccgccagg gcgtaacctg gcacgatgga cagccgttca ccgcggccga
     1861 cgtcaagttc acctgggaac tgatcgccaa cccgaagaac accaccgccg cccagctcta
     1921 cagcttcttc tccaagatcg ttggcgccga tgacttccac accggcaagg cgaccgagat
     1981 caccgggatc aaggcggcgg acgacctcac gctcgacgtc acactcgctt ctccctgggc
     2041 cccgttcctg acgatcgcct cgaaccagta catcgtcccc aagcacatcc tcggcgatat
     2101 cgccgtcgac aagatcctgg aggatggata cgcgcgggcg ccgatcggca ccggcccctt
     2161 caccttcaag gcgtggcagg cgggggacag catcattggc gaggcgttcg acaactacta
     2221 cggtggccgc ccctacgccg acaccatcgt catgcgggtg gcagggcttg acgccaacgg
     2281 cgaactctcc gccctcgact cgggagagct gaacgagagc gatctcaccc tcgtacaggc
     2341 cgacaccctg acgggagata gtgccgtcgt gctcaacgag cgggcaggcg aggcgaacca
     2401 gtacgtcgag ttcaatctcg cgaagccgct cttccaggac gtcaatgttc gcaaggcgct
     2461 ctcctacgca ctcgaccgca acgcgatctc gcaggcggtc tggcagggtc gcgcccagat
     2521 ctacaactcg gtcttcccgt acgactggtg gtgcacgaag aaggacacca cgatcttcga
     2581 taacgacgcc gatcaggcca agaagctcct tgatgcggca ggctggaccg tcggaagcga
     2641 cggcattcgc gagaaggacg gcacgaagtt ctccttcacc gcctactcga tcagcccgga
     2701 ctggtcgctc gtcgtgcagc agcagtggaa ggcgatcggc atcgacgtca agctgcagat
     2761 ggtcgatttc ccgaagctct cgacgcagta ctacacgacg catctcttcg atgtcgtctt
     2821 cctcaatatc ccgtacaccc tctacacgga tccgcactac tcgctgccgg gctacttcct
     2881 gagtgcgaac aaccgcaacg cctacaagaa cccgaagtcc gacgagctga tcatcgccgc
     2941 cgcctcgacc aacgatcagg agcaacgaaa gcagctctac taccagtggc aggaattgat
     3001 cgcgcaggat gtcccgcacc tctggatcgg gaatccgacc gcgccgacgg catactcggc
     3061 cggtctctac tatccggacc gcagtagcag ttacttctcc tggcgggaga tccgcgactg
     3121 gtactggacg gagtagacgg cagaacgtcg ggttcaagcg cggcaggagc ggacgattgg
     3181 gatcggcacc ggaagcgaca tcgccaggtg gggcagtgac acccctcggc aggggagaga
     3241 gaacggcggc gccggaaacg gggcaggcga ggagaatcct cgtcgcctgg ctccgcaacc
     3301 cgcggctggc ggtcggcggc ctcgtgattg ccgtcgtctg cctgctcgcg atctttgcgc
     3361 cgtgggtgac ccagaccgca cccgatgtcc agaatccgct ctaccggctc gcgaaaccgg
     3421 ggaatcactt cctcctcgga ggtgattcct tcggtcgcga tatctacagc cgcctcgtct
     3481 atgccggtcg cgtctcgctc tcggtcggcg tgctctcgat ggtgatcacc gtcgtcgttg
     3541 gagtgctggt tggcatcgtc gccggatact acggcggctg gatcgaccgc ctcctgatgc
     3601 ggctgaccga tatcgtcctc gtctttccga ccttcttcct gctcgtcttc gtcgtcgcga
     3661 cgttcggcag cagcgtgaag ctgctcatcg tgatgatcgg tctcacctcc tggccaacca
     3721 acgcccgcgt ggtgcgggcg gcggtgctcc ggttgcgcga tcacgagttc attgttgccg
     3781 cgaaggtcaa cggggcgaga ggcagctgga tcatgcagaa gcacctgctc ccgcaactgc
     3841 tcccgatcgt gatcgccagc gcgacgatcc gcgtggcaaa caacatcctc atcgagtcag
     3901 gactcagcta tctcggcctt ggcgtacagc cgccgacgcc gacctgggga aacatgatct
     3961 cggattccgc ggtctacatg cgtcaggcct ggtggttgat ggcgattccg gcaatcacga
     4021 tcttcgtcgt cgtgcttgcc ttcaacctgc tcggtgaggg attgcgcgat cagctcgatc
     4081 cgcgccggag gggacggtaa tccatgcggt actggctcaa tcgcattctc gagatcgtgc
     4141 cgctgttgat cgggatctcg ctgctcacct tcgtcatctt ccagctcacg ccgggtgacc
     4201 cgatcggcct cgtcgtcgat ccaaccctgc tctccgcgga cgagcgatcc gctgtccgcc
     4261 agagcctcgg tctcgacgac ccgctcccga tccagtacgt gaagatgatg gaggggatcg
     4321 tcgatggcaa tcttcgttca ttcaagacga agcagccgac gatcgacatg gtctggacgg
     4381 cctttccgat cacgctggtg atcggcgcag gtgggctgat tctcgccctc ctgatcgggt
     4441 tgcccgttgg atcgctcgcg gcgagacgac ccggtggtat cgccgacaag atcgtctccc
     4501 tcacgctctc gaccagtctc gccactcccc aattcgcact cggactcatc ctcgtcctca
     4561 tcttcacggt gaagctgcat ctgcttccgg gatccgggat cgcgccaatc ggcaccgtcg
     4621 gcttcgacgg cgtccattcg atccgctatc tgatcatgcc ggtcgccgtc atcgcctttg
     4681 gccaggtcgc gatcttcgcc cgctacgtcc gtgacgccct ggtcgacgtc ctcgccaacg
     4741 aatacatccg caccgcccgc gcgaagggcc tcg
//
LOCUS       DASTMD010000777         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2215068, whole genome shotgun sequence.
ACCESSION   DASTMD010000777 DASTMD010000000
VERSION     DASTMD010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2215068"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..422
                     /locus_tag="VFQ54_11540"
     CDS             207..422
                     /locus_tag="VFQ54_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165667.1"
                     /translation="MGTESLKLMDRAINSMHTNRTIKLQRISVEGDRKILIYDPEMNV
                     MQVYDKDSVWMPDATPAEQIIRDAREK"
     gene            425..667
                     /locus_tag="VFQ54_11545"
     CDS             425..667
                     /locus_tag="VFQ54_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165668.1"
                     /translation="MAKFEHAVLTIGVDGSNIISERMYFGREGGVERFVSYPGEDATQ
                     KFDSLFTELGSDGFELVSVVSENEHRQTFYFKRQVS"
     gene            664..873
                     /locus_tag="VFQ54_11550"
     CDS             664..873
                     /locus_tag="VFQ54_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165669.1"
                     /translation="MTPAERRKAAKALMDDAELLNGRARDLLRDLDLPAGEEIVLSDM
                     LYRALHDLRTVTNVLRGKVGKAAET"
     gene            1027..1434
                     /locus_tag="VFQ54_11555"
     CDS             1027..1434
                     /locus_tag="VFQ54_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165670.1"
                     /translation="MELRKSGMSFQEIADDLGYANASGAYKAVMAALKKTLQEPTDEL
                     RHLELNRLDVMLSSLWPAIIAKNQYTPRTVEVALKVMDRRAALLGLDAPQKREDTQRV
                     EVTMLIKRIAEESGLDEHEIIQEAERILQDATS"
     gene            1431..2714
                     /gene="terL"
                     /locus_tag="VFQ54_11560"
     CDS             1431..2714
                     /gene="terL"
                     /locus_tag="VFQ54_11560"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEU0165671.1"
                     /translation="MSAVALPNDPAFYASIAAGIKTRRANDPWDGPNGSARLNQLPPD
                     GSWQTWLIMAGRGFGKTRSGAEWIRKRVQHGVARRIVIAGPTAADIRDIMIEGESGLE
                     AVCERAGVRMVYNPSKRRVTFANGALAVLVSADEPKRFRGLQSDTFWADELASWRYPD
                     AWDQLQLGHRLGNDPQGIVTTTPRPIPIIKDLLKDPDTVVTRGNTFENAANLAQRYLD
                     QITRRYEGTTLGRQELYAEILDDVEGALWSRSLIQHVKPDEVPDLVKIAVGVDPATTY
                     GEKSDETGIAVAGIDRDGMVYVLEGLGLRVSPAEWAHKALLFHDGHKANWIVAESNQG
                     GEMVRSTILHAVKPGTMRPKIDLLHVSRGKQVRAEPVVALYEQGRVKHVGVFASLEDQ
                     MCSFPVSTDHDDQVDALVHAITAVAPTKSRGFWSA"
     gene            2715..4121
                     /locus_tag="VFQ54_11565"
     CDS             2715..4121
                     /locus_tag="VFQ54_11565"
                     /inference="COORDINATES: protein motif:HMM:NF016988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU0165672.1"
                     /translation="MTLPANPLAWAISAFADGRNDKYVTYEKYLAGDQPLAFATDKFQ
                     STFGQLFHEFAYNRCAAVVDAHADRLQVSGFDATDPDIAKEAQKLWEANQMSVREGHV
                     YVDQFGDGDAYVLVDVHPETGDVQYWTQNPKSIRVHWDDERPNTIDLAAKMWDDDEKL
                     CRVNLYFADRIEKYISAKAAKAGSSASLEDFTPYEVEGESWPVMLAVSDTVPVFHFAN
                     NARTNTYGVSELRSIIPLQDAINKSLMDLFVAMELAAFPQRVLIGIDKPETDEERELL
                     NNFVVGMNKVLTMTDPNAKIAEFSAVNITQYTDVAEYIDKAICRVSKIPVHYLEMTGN
                     FPSGRAQSLASIPFTKKMTDRTKADGAVWGDVQRYGVRLRGFDVKAGDIRVNWESVED
                     TSDDDVWDLITQQINSGVPLVTALRWNGYTEDEITQFEQDLAEYGDGSADFSSSDRPL
                     SGDINPEQASADDEQQAA"
BASE COUNT          934 a         1260 c         1290 g          734 t
ORIGIN      
        1 aacgatttac tgaccaacca ggacccacca cccgtgacgg tgacacctgc ccagcgccgc
       61 gccgtgatcg aggagctggc gcgcgaggcg gacctgctgg agatggtcgt cggcagggat
      121 gacggcgatc ctgcctacgg ctaggcgtac aacatctccg agacactggg cgactggctg
      181 cgtgcgcata tcgaggagga ggggtgatgg gcacagaaag cctgaagctc atggaccggg
      241 cgatcaattc gatgcacacc aatcgcacga tcaagctcca gcgaatcagc gtggagggcg
      301 ataggaagat actgatctac gatcccgaga tgaacgtgat gcaggtctac gataaggatt
      361 cggtctggat gcccgatgca acaccagccg agcagatcat ccgggatgcg agggagaaat
      421 aacagtggca aagtttgagc acgcagtcct gacgataggc gtggacggat ccaacatcat
      481 tagtgagcgc atgtactttg gtcgcgaagg aggcgtcgaa cggttcgtct cctatccggg
      541 cgaggacgcg acacagaagt tcgattcgtt atttaccgag ctgggaagtg atgggtttga
      601 gctcgtttcg gttgtgagtg agaacgagca ccggcagacg ttctacttca agagacaggt
      661 gtcatgaccc ccgctgaacg ccgcaaggcc gccaaggcgc tcatggatga cgccgagctg
      721 ctcaacggtc gggcgcgcga cctcctccgc gatctcgacc ttcccgccgg cgaggagatc
      781 gtgctcagcg acatgctcta ccgtgcactc cacgatctgc ggaccgtcac aaacgtgctg
      841 cggggcaagg tggggaaggc ggccgagacg tagccgtctg atatactgag tgtgccgatc
      901 tgggggaacg gacgcgtagc gtgtctggat tcccggatgg cggattcccc atcacaaccc
      961 aagtacagca ggaaacccag ggttggaaaa aacgaccctg aatccgtcga ccgccaacga
     1021 tctgccgtgg aactccggaa gtccggaatg agtttccagg agatcgccga cgaccttggg
     1081 tatgccaacg cctccggtgc atacaaagcg gtcatggccg cactcaagaa aacgctccag
     1141 gaaccaaccg acgaacttcg tcacctcgaa ctcaatcgac ttgacgtaat gctctcgtca
     1201 ctctggcccg ccatcattgc caagaaccag tacacaccac gaaccgttga agtcgccctc
     1261 aaggtgatgg accgacgtgc ggcgcttctc ggtctcgacg ctccccagaa gcgggaggat
     1321 acccaacggg tcgaggtcac catgctcatc aagcgcatcg ccgaagagtc gggacttgat
     1381 gagcacgaga tcatccagga ggccgaacgc atcctccagg acgcaacatc atgagcgctg
     1441 ttgcccttcc caacgacccg gcgttttatg cctcgatcgc tgccgggatc aagacgcgcc
     1501 gggccaacga tccatgggat ggaccgaatg gatcggcgcg tctgaaccaa cttccaccgg
     1561 atggatcgtg gcagacgtgg ctcatcatgg cgggtcgcgg attcggcaag acacgatcag
     1621 gtgccgagtg gatacgcaag cgtgttcagc acggggttgc tcgccgcatt gtgattgccg
     1681 ggccaaccgc ggcggacatc cgcgacatca tgatcgaagg ggagagcgga ctcgaggcgg
     1741 tgtgcgagcg tgccggcgtt cggatggtct acaacccatc gaagcggcgc gtcacctttg
     1801 cgaacggcgc gctggctgtg ctggtctccg ctgacgaacc gaagcgattt cgcggtctac
     1861 agagcgatac gttctgggcg gacgagcttg cgtcatggcg ctaccccgac gcatgggatc
     1921 aacttcagct cggacaccgg ctcggaaacg acccacaggg catcgtgacc acaaccccac
     1981 gtccgatccc catcatcaag gacttgctca aggatcctga cacggtcgtc acacgtggca
     2041 acacctttga gaacgccgcg aatcttgccc agcgctatct cgaccagatt acccggcgtt
     2101 acgaaggcac cacgctcggg cgccaggagc tctatgccga gatcctcgat gatgtcgagg
     2161 gcgcgctttg gagccgatca ctgatccagc acgtgaagcc ggacgaagtt ccggatctcg
     2221 tgaagatcgc agtcggcgtc gatcccgcga cgacgtacgg cgagaaatcc gacgagacgg
     2281 gtattgctgt ggccggcatc gatcgcgacg gaatggtcta cgtccttgag ggactgggcc
     2341 ttcgtgtctc gccggcagag tgggcacaca aggcgctgct cttccatgac ggccacaagg
     2401 cgaactggat cgtggcggaa tccaaccagg gcggggaaat ggtgcgctcc acgatcttgc
     2461 acgcggtgaa accgggaacg atgcgaccca agatcgacct cctgcacgtc agccgaggca
     2521 agcaggtccg cgctgagccc gtcgtcgcct tgtacgagca aggacgagtg aagcatgtcg
     2581 gcgtgtttgc ctcactcgag gaccagatgt gctcgttccc cgtatcgacc gatcacgacg
     2641 atcaagtgga cgcgctcgtt catgccatta ccgctgtcgc cccgacgaag tctcgtggat
     2701 tctggagtgc ctagatgacc ctgccagcca acccccttgc ctgggccatc agtgcctttg
     2761 ctgacggacg caacgacaag tacgtcacgt acgagaagta cctggccggc gaccagccgc
     2821 tcgccttcgc gaccgacaag ttccagtcga cgttcggcca gctcttccac gagttcgcct
     2881 acaaccgctg tgccgccgtc gtggatgccc atgcggatcg cctccaggtc tcagggttcg
     2941 acgccaccga cccggacatc gccaaagaag cccagaagct ctgggaagcc aaccagatga
     3001 gtgttcgcga gggccacgtc tatgtcgatc agttcggcga cggcgacgca tacgtgctcg
     3061 tggatgtcca tccggagacg ggcgacgtcc agtactggac gcagaacccg aagtcgatcc
     3121 gtgtccactg ggatgacgaa cgtcccaaca ccatcgacct cgccgccaag atgtgggatg
     3181 acgacgaaaa gctctgtcgg gtcaacctct acttcgccga ccggatcgag aaatacatca
     3241 gtgccaaggc agcgaaagca ggatcgtccg catcacttga ggacttcacg ccgtacgagg
     3301 tcgagggtga atcgtggccg gtgatgctgg cggtcagcga tacggtgccg gtctttcact
     3361 tcgcaaacaa cgcacggacg aacacgtacg gtgtcagcga actccgctcg atcattccgt
     3421 tgcaggatgc gatcaacaag agcctgatgg atctcttcgt ggcgatggag ctggccgcgt
     3481 tcccacaacg ggtgctgatt ggcatcgaca agccggagac ggatgaggaa agggagctgc
     3541 tcaacaactt cgtggtgggc atgaacaaag tgctcacgat gaccgatccc aacgccaaga
     3601 tcgccgagtt ctccgcggtg aatatcacgc agtacacgga cgtcgcggag tacatcgaca
     3661 aggcgatctg ccgggtttcc aagattccgg tccactacct ggagatgacc ggaaacttcc
     3721 cctctggccg agcacaatcg ctcgccagca tcccgttcac caagaagatg accgaccgca
     3781 cgaaggcgga tggcgccgtc tggggagacg tccagcggta cggggtacgt ctgcggggat
     3841 tcgacgtcaa ggccggcgat atccgcgtca actgggaaag tgtggaggac acgtccgacg
     3901 acgatgtctg ggacttgatt acccagcaga tcaacagcgg cgtgccactc gtcacggctc
     3961 ttcgctggaa cgggtacacc gaggacgaga tcacgcaatt cgagcaggac ctcgccgaat
     4021 acggtgacgg gagcgcggac ttctcgagtt ccgatcggcc actgagcggc gacatcaacc
     4081 ccgaacaggc aagtgctgac gatgagcagc aggctgccta gccatggtcc tgaaccctgc
     4141 tcaatctgac ctcgtcgacc tgatccggca ggcgttggcg cggaacgaca aggtcttcgc
     4201 gaagttctgg acggaggt
//
LOCUS       DASTMD010000778         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_785653, whole genome shotgun sequence.
ACCESSION   DASTMD010000778 DASTMD010000000
VERSION     DASTMD010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785653"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFQ54_11570"
     CDS             complement(<1..718)
                     /locus_tag="VFQ54_11570"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU0165673.1"
                     /translation="MPKRPTGHNAFVEEFEDEIEDGPEGERLQRVLAARGIASRRASE
                     TMITDGRVAVNGVVVTTLGTRVDPLKDELRVDGKVLRRQRPRYLMLNKPSGFITTVND
                     ERQRWTVMDLIQVQERIYPVGRLDRDTQGLLLLTNDGTLANRVMHPRYGLTKEYHVIT
                     NVRPSSEQLQDVKDGLLIDGRLVIPDECRLMRETNEGIIIKIVLHEGLYHVVRRMMEL
                     VQIEVVRLRRARLGPLTIQGI"
     gene            complement(761..>1648)
                     /locus_tag="VFQ54_11575"
     CDS             complement(761..>1648)
                     /locus_tag="VFQ54_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165674.1"
                     /translation="EDDDMAAAGYSTMGARVGDQISVANLLAGLLIPSGGDAANALSR
                     YVGGKLSGETDVDTARSAFVSSMNEYVQSTLKLKNTNFTDPAGDDDSSGYSTAMDLMQ
                     VGVKIMADPDLSWIVGQYTVSVPSQNGDPYPLTSTNDLLKDSSNGIVGIKTGSTDGAG
                     ACLLSARKVNNGANTAIIVVLGSSLKYDGNGDIVKDSDGRFTDTEAIIGDMDSRFTWN
                     TADQVETLFPDLKDQLSVWGVEFKDPPVVPIATGAKDKPTCQLVLGKATDAGKQAGSV
                     DFYSGSDITGSIPVYQVGG"
BASE COUNT          287 a          515 c          496 g          350 t
ORIGIN      
        1 gaatcccctg gatcgtgagc ggaccgagcc gggcccggcg cagccgcacg acttcgatct
       61 ggacgagctc catcatccgc cgcacgacgt ggtagagacc ttcgtggagg acgatcttga
      121 tgatgatccc ctcgttcgtc tcccgcatca gccggcactc gtcgggaatg acgaggcgac
      181 cgtcgatcag caggccgtcc ttgacgtcct gaagctgttc agacgagggc cggacattgg
      241 tgatgacgtg gtattccttg gtcaggccgt agcggggatg catgacacgg ttggcgagcg
      301 tgccgtcgtt cgtgagcagg agcaatccct gcgtatcgcg atcgagccgc ccaacggggt
      361 agatgcgctc ctggacctgg atcaggtcca tcaccgtcca ccgctggcgc tcgtcgttga
      421 cggtcgtgat gaagccgctc ggcttgttca gcatcaggta gcggggccgc tgccgccgca
      481 ggaccttgcc gtcgacccgt agctcatcct tgagcggatc gacgcgcgtc cccagcgtcg
      541 tgaccacgac gccgttcacg gcgacgcggc cgtccgtgat catcgtttcc gaggcgcgtc
      601 gcgaggcgat gccgcgcgcg gcaagcaccc gctgcaggcg ctctccttcg ggaccgtcct
      661 cgatttcatc ctcgaactct tcaacaaatg cattgtgtcc tgtaggtcgt tttggcatgt
      721 atgccgccgt ctcaatcttc ccgaaggtct tgccgagatc ctatccacca acctgataga
      781 cgggtatcga tccagtaatg tcgctgccgc tgtagaaatc caccgatccg gcctgcttcc
      841 cggcgtcggt cgccttgccg aggacgagtt ggcaggtcgg cttgtccttc gcgccggtgg
      901 caatcggcac caccggcggg tccttgaatt cgactcccca gacgctcaac tgatccttga
      961 gatcggggaa gagcgtctcg acctgatccg ccgtgttcca ggtgaaccgg gaatccatgt
     1021 cgccgatgat cgcctcggta tccgtgaatc gcccgtccga gtccttgacg atgtcgccgt
     1081 tgccatcata tttgaggctg gatccgagca cgacgattat cgccgtgttg gcgccattgt
     1141 tgacctttcg cgcggagagg aggcaggcac cggcaccgtc ggtcgaaccg gtcttgatgc
     1201 cgacaattcc gttgctcgaa tccttaagca ggtcattcgt gctcgtcagc gggtacggat
     1261 cgccattttg tgagggaaca ctcacagtgt attggccaac gatccagctt aggtcgggat
     1321 ctgccatgat cttcacgccg acctgcatga ggtccatcgc agtcgaatac cccgaagagt
     1381 cgtcgtctcc ggcgggatcg gtaaagttgg tgttcttgag cttgagtgtc gactggacgt
     1441 attcattcat cgagctgacg aaagcggacc gcgccgtgtc gacatccgtc tcaccgctca
     1501 gctttccacc gacgtaccgc gagagtgcgt tggcagcatc gcctcccgat gggatcagga
     1561 gaccggcgag gagattggca accgagattt gatcgccgac ccgcgcgccc atcgttgaat
     1621 acccggccgc cgccatgtcg tcatcttc
//
LOCUS       DASTMD010000779         1840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1054494, whole genome shotgun sequence.
ACCESSION   DASTMD010000779 DASTMD010000000
VERSION     DASTMD010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054494"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /gene="hisH"
                     /locus_tag="VFQ54_11580"
     CDS             <1..661
                     /gene="hisH"
                     /locus_tag="VFQ54_11580"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEU0165675.1"
                     /translation="PHGGAPDRRRRQHPIHERRARMIAIIDYDAGNLRSIQRALLETG
                     ADVTITSDPEVVVKADRVVFPGDGHAKTAMDTLDERGLTAAIHEFVAAGKPFFGVCVG
                     MQLLFEGMEEGPATGLGLLSGSVRKLPSAVKVPQMGWNIVSFAEGSPLSYLGDSYFYF
                     VHSYAPEAFDRSDVVGETDYGVRFPSVVINDNIWGTQFHPEKSGEDGLSLVHTWAAWN
                     P"
     gene            794..1528
                     /gene="hisA"
                     /locus_tag="VFQ54_11585"
     CDS             794..1528
                     /gene="hisA"
                     /locus_tag="VFQ54_11585"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF010112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEU0165676.1"
                     /translation="MIIYPAIDIRGGRAVRLVEGDFDRETTFDADPVDAAKRWADAGA
                     EWIHIVDLDGAKDGTGANRDAVRRIRDAVSVPLELGGGIRTTGDIKELTDIGVDRLIL
                     GSIAITNPEFVEEAIAAHGDAIAVGLDARNGKLAANGWLDQTEVDALDAAKRFAAAGL
                     KTVIFTDIHRDGTFQGPNIDALSTLIREVPIDVIASGGVGALDDVQNIAAAGASGVII
                     GSALYHKKIALTDALRIGRETIGVAR"
     gene            1525..>1840
                     /locus_tag="VFQ54_11590"
     CDS             1525..>1840
                     /locus_tag="VFQ54_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEU0165677.1"
                     /translation="MNPVATANNLTVRVIPCLDVTNGRVVKGVEFLDLVDAGDPVEMA
                     KVYYREGADELTFLDITASSDSRETMVDVVRRTADQLFIPLTVGGGIRTVADMKKMLH
                     AGA"
BASE COUNT          317 a          651 c          554 g          318 t
ORIGIN      
        1 tccgcacggc ggtgcgccag accggcgtcg ccggcagcat cccatccacg aaaggcgtgc
       61 tcgcatgatc gcgatcatcg actacgatgc gggaaatctc cgctccattc agcgcgccct
      121 tctcgagact ggcgccgatg tcaccatcac ctccgatccc gaggtcgtcg tgaaggcgga
      181 tcgggtggtc ttccccggcg acggccacgc gaagacggcg atggacaccc tcgacgaacg
      241 tggactgacc gcggcgattc acgagttcgt cgccgccggc aagccgttct tcggcgtgtg
      301 cgtcggcatg cagctgctct tcgaggggat ggaggaaggc ccggcgacgg gtcttggcct
      361 gctctccggc agcgttcgca agcttccctc cgccgtgaag gtcccgcaga tgggctggaa
      421 catcgtcagc ttcgcggagg gaagtcccct ctcctatctc ggcgattcct atttctactt
      481 cgtccactcc tacgcccccg aggcgttcga tcgctccgac gtggttggcg agacggacta
      541 cggcgtccgc ttccccagcg tcgtgatcaa tgacaacatc tggggaaccc agttccaccc
      601 cgagaagagc ggcgaagacg gcctctccct cgtccatacc tgggccgcct ggaatccgta
      661 gcgctcggct ggcatatccg tcgggacgtc ttgccccgta tctctccccg acaagcctgt
      721 caccccgaac cgtagatgcg acccttgtgg tcgccgcgcc gcgcagcggc gcccctcctg
      781 aaggacaaag cgcatgatca tctatccagc catcgacatc cgaggcggtc gcgctgtccg
      841 cctcgtcgaa ggtgacttcg accgtgaaac gaccttcgac gccgatcccg tcgacgctgc
      901 gaagcgttgg gccgacgcgg gcgccgagtg gatccacatc gtcgatctcg acggcgccaa
      961 ggacggaacc ggtgcgaatc gcgacgccgt ccgccggatc cgggacgcgg tctccgtgcc
     1021 gctcgagctc ggcggtggca tccgcacgac gggcgacatc aaggaattga ccgacattgg
     1081 tgtcgatcgc ctgatcctcg gctcgatcgc gattaccaat ccggagttcg tcgaggaagc
     1141 gatcgcggcc catggcgacg caatcgctgt tggtctcgac gcccgcaacg gcaagctcgc
     1201 cgccaacggc tggctcgacc agaccgaggt cgatgcgctc gatgccgcga agcgcttcgc
     1261 cgcggccggt ctcaagacgg tcatcttcac ggacatccac cgcgacggca ccttccaggg
     1321 accgaacatc gacgccctct cgaccctgat tcgcgaggtt ccgatcgacg tcatcgcctc
     1381 cggtggcgtc ggcgcgctcg atgacgtcca gaacatcgcc gccgcgggcg catccggtgt
     1441 catcatcggc agcgccctct atcacaagaa gatcgccctg accgacgccc tccgcatcgg
     1501 ccgcgagacg atcggagtcg cccgatgaac cccgtcgcca cagccaacaa tctgaccgtt
     1561 cgcgtcatcc cctgtctcga cgtcaccaac ggtcgcgtcg tcaagggcgt cgaattcctc
     1621 gatctcgtcg acgccgggga tccggtcgag atggcgaagg tctactaccg cgagggtgcc
     1681 gacgagctca cctttctcga catcaccgcg tcatcggata gccgcgagac gatggtggat
     1741 gtcgtgcgtc gcaccgcgga ccaactcttc atcccgctca ccgttggcgg cggcatccgc
     1801 accgtcgccg acatgaagaa gatgctccac gcgggtgccg
//
LOCUS       DASTMD010000780         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2394413, whole genome shotgun sequence.
ACCESSION   DASTMD010000780 DASTMD010000000
VERSION     DASTMD010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2394413"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFQ54_11595"
     CDS             <1..208
                     /locus_tag="VFQ54_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU0165678.1"
                     /translation="ALYHDGFDPEITRQLHAEGIGIWAWTVNDAERMKTLASWGADAV
                     CTDDPELGVSLFATGDVTPIQESR"
     gene            complement(221..1036)
                     /locus_tag="VFQ54_11600"
     CDS             complement(221..1036)
                     /locus_tag="VFQ54_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU0165679.1"
                     /translation="MTTHFSEHLNWGSLSFGDLLLRLGLATVLGLLVGVERERLERAA
                     GMRTHAVVCVGAALITIVSIYGFPAPGGEQAVSLDPSRIAAQIVSGVGFIGAGVIFMR
                     RDVVRGLTTAASVWAISGVGITAGAGLYIPAAVGAGFMLLIQRGLRPLERRFFRHQAT
                     QHDVEIVASHAPDAVARIRQLLMGSSIHLHAIDVDRGGDGKADTIHLNLRTEKTDEIL
                     SLVDLLQDTDGVRGVRWRHGAAQRRNQGSGTGGGGGRDPGEGDAAPGDDDGAS"
     gene            complement(1196..2527)
                     /locus_tag="VFQ54_11605"
     CDS             complement(1196..2527)
                     /locus_tag="VFQ54_11605"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HEU0165680.1"
                     /translation="MPARIDRALVRSALQGATTTRHLTVTPGAVDDAGALFIAAFGDR
                     PAIVIADETTFAIAGERVQRSLRAAAISMTRPLILPVTVSPDYADVTAIRQTISAAPP
                     ETIPIAVGAGTINDLVKRAAFEHGLPYMAVATAASMDGYTASGASLLVDGIRQTVDCP
                     APVAVVGDTEILAAAPGAMTAAGYGDLVGKITAGADWLLADALGIEPIVLQVWSMVQD
                     PLRATVAHPERLHETGNEAAIEQLFLGLAVAGLAIQATGSSRPASGSEHQLAHFDEMM
                     HPLSTLPHGFRVALGTLAATQLYEQLLAMPLDHLDPERASAHWPSLAEQEDEVRRTQP
                     TPRMVDRALEEVRAKYASPAEVTRRLQAIRAAWPALSQNLEAQLIPSDELRILLATAG
                     CPTIPEGSSLTDRDLRASYIGARQIRRRYTVLDLAFETGVLTTHESTSTRR"
     gene            complement(2660..>3211)
                     /locus_tag="VFQ54_11610"
     CDS             complement(2660..>3211)
                     /locus_tag="VFQ54_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0165681.1"
                     /translation="FARLRFRFDKALFAMVLATMIVPGVVTVVPNYVMISRLGWMNSW
                     MGVIIPSLPATAFYVFLLRQTILTLPQDLIDAAKLDGASTIKLLWQIVIPNVRPAIAV
                     VTVLSFLGAWNQYLWPLLVLNDFDKKTLATGMQFFTSNAESAQMWGPMMATATIAALP
                     PLIVYALAQKQIISTFVTSGFKG"
BASE COUNT          550 a         1016 c         1128 g          517 t
ORIGIN      
        1 cgcgctctac cacgacggat tcgaccctga gatcacccgg caactccacg ccgagggtat
       61 cggcatctgg gcctggacgg tgaacgacgc cgagcgaatg aagacgcttg cctcgtgggg
      121 tgccgatgcc gtctgcacgg atgatccgga actgggcgtc agtctcttcg cgacgggtga
      181 cgtgaccccc atccaggagt cgcgatgaac gaggtggcgc tcacgaagcc ccatcgtcgt
      241 ctccgggggc ggcgtctcct tcaccagggt ctcgtccacc gccgccaccc gtaccggagc
      301 cctgattgcg acgctgggcc gccccgtgtc tccaccggac accgcgaacc ccatccgtat
      361 cctgcagaag atcgacgagg gagagaatct cgtcggtctt ctccgtgcgc aggttgaggt
      421 ggatcgtgtc tgccttgcca tccccgccgc ggtcgacatc gatcgcgtga aggtggatgg
      481 acgagcccat aaggagctga cggatcctgg cgacggcatc cggcgcgtgt gacgcgacga
      541 tctcgacgtc gtgctgcgtc gcctggtggc ggaagaaccg gcgttcgagc gggcgcaatc
      601 ctctctggat caacagcatg aacccggcgc cgacggcggc cgggatgtag agaccggcac
      661 ccgccgtgat cccgacgccg gagatcgccc agacactcgc cgccgtggtc agcccccgca
      721 cgacatcgcg tcgcatgaag atgacaccgg cgccgatgaa gccaacgccg ctcacgatct
      781 gcgccgcgat ccggctggga tcgagcgaga cggcctgctc gccgccaggc gccggaaagc
      841 cgtagatcga gacgatcgtg atcagcgccg caccgacgca gacgacggca tgcgtgcgca
      901 ttccggcggc gcgttccagc cgctcccgct cgacgccgac gagaagaccg agcactgtcg
      961 cgaggccgag tcggagcagg agatcgccga agctgagcga tccccagttc agatgctccg
     1021 agaagtgagt ggtcacgcgg cgttgacctt tcgttaacgc gcatcgagcg aagaaaatcc
     1081 cccgggggat gggagatgtc acctcatctt cgtgtgcacg atccgggccg ccttgcccgg
     1141 atgccgtgac atcgtccggc ttccacgctg cggcggaacg caatcctgtg cgggatcagc
     1201 gtctggtcga cgtgctctcg tgggtcgtga ggacgccggt ctcgaaggcg agatcgagca
     1261 ccgtgtagcg acggcggatc tggcgggctc cgatgtatga cgcccggaga tcccgatcgg
     1321 tgagactgga cccctcgggg attgtgggac agcctgctgt cgcgagcaga atccggagct
     1381 cgtcggacgg gatcagttgc gcctcgaggt tctggctgag cgccggccac gcggcgcgaa
     1441 tcgcctgcag gcgacgggtc acctccgccg gagaagcgta cttcgcccgg acctcctcca
     1501 gggcgcgatc gaccatccgc ggcgtgggtt gggtgcgacg gacctcgtcc tcctgctccg
     1561 cgagcgatgg ccagtgcgcg ctcgcgcgct cgggatcgag atggtcgagc ggcatggcaa
     1621 ggagctgctc gtagagctgc gtcgcggcga gcgtgccgag ggcgacccgg aagccgtggg
     1681 gcagggtgct cagcgggtgc atcatctcgt cgaagtgcgc gagttgatgc tcgctgccag
     1741 aggcggggcg tgacgatccc gtcgcctgga tcgccaggcc ggcgacggca aggccgagga
     1801 agagttgctc gatcgcggct tcattgccgg tctcgtggag acgctccgga tgggctaccg
     1861 tcgcccgcag cggatcctgc accatcgacc agacctggag gacgatcggt tcgatgccga
     1921 gtgcgtccgc gagcagccag tcggcgccgg cggtgatctt gccgacgaga tcgccgtaac
     1981 ccgcggccgt catcgcgccc ggtgccgcgg cgagaatctc ggtgtcgccg accacggcga
     2041 ccggcgccgg gcaatcgacc gtctgcctga tgccgtcgac gaggagggag gcgccggagg
     2101 ccgtgtagcc atccatcgat gccgcggtcg ccacggccat gtacgggagg ccgtgctcga
     2161 aggctgcgcg tttgacgaga tcgttgatcg tgcccgcgcc gaccgcgatc gggatcgtct
     2221 ccgggggagc agctgagatt gtctgccgga tcgccgtcac gtccgcgtaa tccggggaga
     2281 ccgtgaccgg gaggatgagc ggacgggtca tcgagatggc ggccgcacgc agggaccgct
     2341 gcactcgctc gccggcgatg gcgaaggtcg tctcgtcggc aatcacgatc gccggacggt
     2401 caccgaacgc ggcgatgaag agcgcgccgg catcgtcgac cgcgccgggt gtcaccgtga
     2461 ggtggcgagt tgtggtcgcg ccctggagcg cggagcgaac gagcgcgcga tcgatgcgtg
     2521 cgggcattgt ctttccagac ccttccgaat ttcgatggac ccgttgctcg tccatgagcg
     2581 tgaatccggt gtaggtcccc ctgcttgtgc agtctgcgcg gtcaccggaa ggggcggatc
     2641 gcgtccaccc ccttccgagc taccccttga atccggatgt gacgaaggtc gagatgatct
     2701 gtttctgggc gagggcatag acgatcagcg gtggcagggc ggcgatcgtc gcggtcgcca
     2761 tcatcggtcc ccacatctgc gccgactcgg cgttggaggt gaagaactgc atgccggtcg
     2821 cgagcgtctt cttgtcgaaa tcgttgagaa cgagcagcgg ccagaggtac tggttccagg
     2881 cgccgaggaa tgagagcacg gtcaccacgg cgatcgccgg gcggacattc gggatcacga
     2941 tctgccagag gagcttgatc gtcgacgcac cgtcgagctt cgcggcgtcg atcaggtcct
     3001 ggggcagggt caggatcgtc tgccggagca ggaagacata gaaggcggtc gccgggagcg
     3061 aggggatgat cacgcccatc caactgttca tccagccgag acgactgatc atcacgtagt
     3121 tcgggacgac cgtgacgaca cctggcacga tcatcgtggc gaggaccatc gcgaagagcg
     3181 ccttgtcgaa tcggaaacgc agccgggcga a
//
LOCUS       DASTMD010000781         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1590820, whole genome shotgun sequence.
ACCESSION   DASTMD010000781 DASTMD010000000
VERSION     DASTMD010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590820"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1846)
                     /locus_tag="VFQ54_11615"
     CDS             complement(209..1846)
                     /locus_tag="VFQ54_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0165682.1"
                     /translation="MTSNASRRRRATGVLAVFVLSLALAACSSGSSDADADSETPIVV
                     GVSALGTKWGTPDSGQDGTLFGNLHAGLVRKAYVAADGDYVGGQDVYSFEPYLAESYD
                     VSDDGLVYTFHLRDDVESSSGNALTADDVVYSYERKFASPAGTTIAVTAPVLTSADQV
                     QKVDDATISITIPSASFGTQLLALLSDYTGGIYDSTLLKEHETADDPYAIEWSDTEPN
                     YGFGPYVVDDYREGQYIDYVKNPGFPADLGTGAERFRYTLIADAGTQLSALRAGDIDI
                     AMGLLPADVSALESEDGITVPLVETPNEWLLIPTVTTSAPFDNVAVRQALAYAIPYDE
                     LIDSVYYGRASKVEYGPISTQTPGYSSDGLEGYNYDIDRAKELLAEAGYSDGVSFTLT
                     IDSSRADLEAAAILIQTRAAEAGFDISIEKVSNAALWEGITAKSFQAFMMRDYAITQT
                     QSYVMRLFTQPGSFANLGNWEDDEYYAAEEAAEAVPDQLSAEAGAEWAKAEAILLEQF
                     PINYALQIQPTVAYASDISGYVWRTDNYLDLTQITRE"
     gene            complement(1952..>2644)
                     /locus_tag="VFQ54_11620"
     CDS             complement(1952..>2644)
                     /locus_tag="VFQ54_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165683.1"
                     /translation="FFFGTDSSGLDVFSRTIAAARIVLGIGIMASVLVVLLACVAGSM
                     LGFFEHRRSAVGVAARLALRLIDLLQSIPLTVAALVIVVIFGTNPIVLGAGIAVILSP
                     AQARLVRTEVLRVRREGYIDAARMAGRSETNIAFTRVLPASLWPLLENWPMLFANAIM
                     IVAALGFLGVGIQPPTAEWGAMISSGSADASAGRWWPVVFPSLALIFSVVTMNLLRRL
                     VVGWLPEHLRGR"
BASE COUNT          495 a          844 c          833 g          472 t
ORIGIN      
        1 gcgatgtttc ccgacgactt ctccggctta tcgttcttgt ttcgccacgg taacgcggca
       61 tctggaggac tcttaatacc tggtcagagg taataaaatt ggcgcgttcg caaccataat
      121 gtcggcgcac cgcccctcgg ctgcgcaccg acggatacgg gcgggaccgg tgaccggtcc
      181 cgcccgtatg acggctcatc cgatcgagtc actcccgagt gatctgcgtg agatccaggt
      241 agttgtccgt gcgccagacg tagccgctga tatccgacgc ataggcgact gtcggctgga
      301 tctggagcgc ataattgatc gggaactgct ccagcagtat cgcctccgct ttagcccact
      361 cggcccccgc ctcggcggat aactggtccg ggaccgcttc agcggcctcc tccgccgcgt
      421 agtactcgtc atcctcccag ttacccaggt tggcgaacga accgggctga gtgaagaggc
      481 gcatgacata gctctgagtc tgcgtgatcg catagtcgcg catcatgaaa gcctggaacg
      541 acttcgcagt gatcccctcc cacaatgcgg cgttcgagac cttctcgatc gagatatcga
      601 atcccgcctc ggctgcccgt gtctgaatga ggatggcggc cgcctcgagg tccgcgcggc
      661 tcgagtcgat agtcagggtg aacgagacgc cgtcgctgta ccccgcctcg gccagcagtt
      721 ccttcgcgcg atcgatgtcg tagttgtaac cctccagacc gtcggaggag tatcccgggg
      781 tctgcgtgct gattgggccg tactcgacct tggaagcccg accgtagtag acactgtcga
      841 tgagctcgtc gtaggggatt gcgtaggcca acgcctgtcg taccgcgacg ttatcgaacg
      901 gggcgctcgt cgtgactgtc ggaatcagca gccactcgtt cggcgtctcc accaatggca
      961 ccgtaatgcc gtcttcactc tccagcgccg acacgtcggc cggcagcaga cccatcgcga
     1021 tgtcgatgtc cccggcccgc aacgcagaga gttgcgttcc ggcatcggcg ataagggtgt
     1081 accggaaccg ctcggccccg gtccccagat ccgcagggaa ccccgggttc ttgacgtagt
     1141 cgatgtactg cccctcgcgg tagtcgtcga ccacgtacgg gccgaacccg tagttgggct
     1201 ccgtgtccga ccactcgatc gcgtaagggt cgtccgcggt ctcgtgctcc ttcaggagcg
     1261 tggaatcgta gatgccgccg gtgtagtccg acaacagagc cagcaactgc gtgccgaacg
     1321 atgcgctcgg gatcgtgatc gaaattgtcg cgtcgtccac cttctgcacc tggtccgcgc
     1381 tcgtgagcac cggggcggtc actgcgatgg tggtaccggc cggggacgcg aacttccgct
     1441 cgtaggagta gaccacgtcg tccgcggtga gggcattgcc cgacgagctc tcgacgtcgt
     1501 cgcgcaggtg gaacgtgtag accaagccat cgtcgctgac gtcataactc tcggcgaggt
     1561 atggctcgaa cgagtagaca tcctggccgc ccacgtagtc gccgtccgcc gcgacatatg
     1621 ccttacggac gagcccggcg tgcaggtttc cgaacagtgt gccgtcctgg cctgagtccg
     1681 gggtgcccca cttcgttccc aacgcgctga cgccgacgac gatgggggtt tcggaatccg
     1741 cgtcggcatc gctgctgccg ctgctgcacg cggcgagcgc cagcgatagc acgaacacgg
     1801 cgaggacacc ggttgcgcgt cgacgtctcg atgcattgct tgtcatgact tgcctctctg
     1861 tgaatgaacg ttgccgccga cgacgaccgg ggcggcggat ggtgcaaggt gcagatcggt
     1921 ggtgcgggca atacagtctc gacgtggtcg gtcaccgtcc tctcagatgt tcgggaagcc
     1981 atccgacgac gagtcgccgg aggaggttca tcgtgacgac agagaagatc aacgcgagcg
     2041 aggggaagac cacgggccac catcgaccag cggacgcgtc ggcgctcccg ctggagatca
     2101 tcgcccccca ctccgccgtc ggcggctgga tacccacgcc aaggaaccca agtgcggcga
     2161 cgatcatgat cgcgttggcg aacagcatcg gccagttctc caacagcggc cacaacgagg
     2221 cgggcaggac gcgtgtgaaa gcgatgttcg tctcggaccg gcccgccatc cgagcggcgt
     2281 cgatataccc ttccctccga acccgaagca cctcggtgcg caccagacgc gcttgggctg
     2341 ggctcaggat caccgcgatc ccagccccga gcacgatcgg atttgtgccg aagatgacga
     2401 cgattacgag cgcggccacc gtcagcggga tcgactgaag caggtcgatg agtcgcaatg
     2461 ccaggcgggc ggcgacgccg accgcgctcc gtcggtgttc aaagaagccg agcatagatc
     2521 cggcaacaca tgccagaagg acgaccagaa cactcgccat gatcccgatg cccagaacga
     2581 tgcgggcggc ggcgatcgtg cgggagaaca cgtcgagacc ggaggagtcc gtaccgaaga
     2641 agaa
//
LOCUS       DASTMD010000782         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2215568, whole genome shotgun sequence.
ACCESSION   DASTMD010000782 DASTMD010000000
VERSION     DASTMD010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2215568"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VFQ54_11625"
     CDS             <1..642
                     /locus_tag="VFQ54_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0165684.1"
                     /translation="NGAGKTTTISMAVGLMRPDAGTAEVFGVDVWKDPLRAKALIGVM
                     PDGFAVPERLTGRELLTFLCLLRGMNPTTVAQRVDDLLAILDFTGSERTLVMEYSAGM
                     RKKIGLATALLHAPQLLILDEPLEAVDPVSAATIKTILHRFVRAGGSVVISSHVMALV
                     EQMCDHVAIINRGKVAAAGRLNDVRGPMSLEERFVSLVTGTSKQIKELSWLGS"
     gene            627..2498
                     /locus_tag="VFQ54_11630"
     CDS             627..2498
                     /locus_tag="VFQ54_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165685.1"
                     /translation="MVGILIRMKLATLRNMPVGIWNWKTAVGLGLALITILLATADET
                     SPSITTGLLAMAFGSWTLGWVLAPIQTGAGTDEPLMPEHFALLPIPSHKLAFGLLVTT
                     FVGIGPLVTLFAFTSLTVFGLRLGVIPTLVGLAAMVLQLTLPILLSRVVIGAASAAMK
                     SRFGIEFAALQYAIIISLSTVGWVIPAALARNHDLFTGPKALVSDNLPSSLWYAVRIL
                     PSGWGPVAVQAARDSHWMLMFACLGGLLILDLLLLLIWSEQLSRRITGRGANAATQMV
                     SLPVGTSRMLPETPLGSAVQNEIRNWFREPRNALELRIAILASLFIVAIPAAVNWTAI
                     VPWVGTIIALLAAACACNLYGIAGSSFWLTLMTPGAEKNDVRGKQLAFLAVFAPAAIL
                     FSTIFTAWGEQTWAWPWILSIMPATLGAAAGVMIVISIRGVVPVPETARKSGNLMAAA
                     DNTAQTFVAMIATALAAIPPAAVVFSGRSLDSRIVEWFAVPIGVGTGALAAWWGGSWA
                     IRDLEREGPELLSILRYGKRASTRATTGGLLDGLPKGRNGIIVLLCWSTFWLPLFPQG
                     LLPLFLKLIGTETKSWCLALYLPDAYQWPVIFGMIALGAILAAVATALQLREPGPRD"
BASE COUNT          405 a          901 c          800 g          491 t
ORIGIN      
        1 aacggcgcgg gcaagaccac gacgatctcg atggccgtcg ggttgatgcg acccgatgcc
       61 ggtaccgccg aggtcttcgg tgtcgatgtc tggaaggatc cactccgcgc gaaagccctg
      121 atcggcgtca tgccggacgg attcgccgta cccgagcggc tgactggccg cgaactcctg
      181 acctttctct gcctccttcg cggcatgaat ccaacaacgg tcgcccagcg cgtcgacgat
      241 ctcctcgcga tcctcgattt caccggttcc gaacgcacgc tcgtcatgga atactccgcc
      301 ggcatgcgca agaagatcgg tcttgccacc gcccttctcc acgcgcccca gctgctcatc
      361 ctcgatgagc cgctcgaggc cgtcgatccc gtgtccgcgg caacgatcaa gaccatcctt
      421 caccgcttcg tccgcgccgg cggttcggtg gtgatctcga gtcacgtgat ggcgcttgtc
      481 gagcagatgt gcgaccacgt cgcgatcatc aaccgcggca aggtggccgc cgccggtcgc
      541 ctcaacgacg ttcgcggacc gatgagtctc gaggagcgtt tcgtctcgct cgtgacggga
      601 acgtccaagc agatcaagga gctttcgtgg ttgggatcct gatccggatg aagcttgcga
      661 cccttcgcaa catgccggtg gggatctgga actggaagac cgcggtcgga cttggactcg
      721 cgctgatcac gatcctcctc gccaccgcgg acgagaccag cccgtcgatc acgaccgggt
      781 tgctggcgat ggccttcggc tcctggaccc tcgggtgggt cctcgcgccg atccagaccg
      841 gcgccggcac cgacgaaccc ctgatgcccg aacacttcgc gctcctcccg atcccctcac
      901 acaaacttgc gttcggcttg ctcgtcacca ccttcgtcgg gatcggcccc ctggtgacgc
      961 tcttcgcctt cacgagcttg acggtcttcg gactcaggct gggagtgatc ccgaccctgg
     1021 ttggactagc cgcgatggtt ctgcagctga ctctcccgat cctgctgtcg cgtgtcgtga
     1081 ttggcgcggc cagcgccgcg atgaagtccc gcttcgggat cgaattcgcc gcattgcaat
     1141 acgcgatcat catttcactc tcgaccgtcg gctgggtgat tccggcggcg ctggcccgca
     1201 atcacgatct cttcacgggg ccaaaggcgc tggtcagcga caacctcccg tccagcctct
     1261 ggtacgcggt gcggatcctg ccatcgggat ggggcccggt cgccgtgcag gccgcccgcg
     1321 actcccactg gatgctgatg ttcgcctgtc tcggcggact gctcattctc gacctgttgc
     1381 tgctcctgat ctggagcgaa cagctgagcc ggcgaatcac gggccgcggc gccaacgccg
     1441 caacgcagat ggtgtcgctg ccggtcggca cgtcccgcat gcttccggaa acgccgctcg
     1501 gttcagccgt tcagaacgag atccgcaact ggtttcgcga gccccggaac gccctcgaac
     1561 tgagaatcgc cattctcgcc agcctcttca tcgtggcgat tccggccgcg gtgaactgga
     1621 cggcgatcgt gccgtgggtc ggcacgatca tcgcgctcct ggccgccgcc tgcgcctgca
     1681 atctctacgg catcgccggc agttccttct ggctcaccct gatgaccccg ggcgccgaga
     1741 agaacgatgt ccgcggcaag caactcgcct tcctcgcggt cttcgcgccg gccgcgatcc
     1801 tcttctcgac gatcttcacg gcctggggag aacagacctg ggcgtggccc tggattctct
     1861 cgatcatgcc ggccacactg ggcgccgccg ccggtgtgat gatcgtgatc tcgatccgcg
     1921 gtgtcgtgcc cgtgccggag acggcgcgca agagcggtaa cctgatggcg gcggcggaca
     1981 acaccgccca gaccttcgtc gcgatgatcg cgaccgccct cgccgcgatc ccacccgccg
     2041 ccgtggtttt ctccggacgt tcgctggaca gcaggatcgt cgaatggttc gccgtgccga
     2101 tcggtgtcgg caccggtgcc ctcgcggcgt ggtggggcgg atcgtgggcg atccgggatc
     2161 tcgaacggga gggtccggag cttctatcca ttcttcgcta cgggaagcgg gcaagcacca
     2221 gggccaccac cggtggtctg ctcgacggct tgccgaaggg gcgcaacggc atcatcgtgc
     2281 tcctgtgctg gagcaccttc tggctacccc tcttcccgca gggactcctc ccgctcttcc
     2341 tcaagctgat cgggacggag acgaagagct ggtgcctcgc gctttacctg ccggatgcat
     2401 atcagtggcc cgtgatcttc gggatgatcg ccctcggagc gatcctcgcc gccgtcgcca
     2461 ccgcactcca gttgcgcgag ccagggccgc gggactgagc gaaccggacg aggacgcggt
     2521 tgctccggaa cggctatcat cagcggatcg tgcagtcgca cacgcgcaat gccatgcccc
     2581 aaaggagcat ccgcatg
//
LOCUS       DASTMD010000783         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1054996, whole genome shotgun sequence.
ACCESSION   DASTMD010000783 DASTMD010000000
VERSION     DASTMD010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054996"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..2637
                     /gene="serA"
                     /locus_tag="VFQ54_11635"
     CDS             334..2637
                     /gene="serA"
                     /locus_tag="VFQ54_11635"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEU0165686.1"
                     /translation="MTNGRERHDIGAPLPRSLRIAIDLDGVLTEHPAPLAAAANARFD
                     LDLPERAFIDSAGLNVPLEVREWVYSDTGPAALLEPAADAVEFIQRAIDILGEGNALI
                     LTARPENSAAMTVAWLKRHGFPSINVIFADDKMTFARRQGCGFAVEDSERHARNYAAG
                     GVRCYLIDPDGHIDVTDEPEIAHVGGYAEILAELTAQVEAARSQPVSVVNTLPPIVDA
                     LTARPRIVVSDNIHPVARQELESQAEIVDVDGTDVDALLAAVADADALVVRSETQVTE
                     RVLGAGRKLRVVARAGVGVDNIDLGAATRAGVLVLNAPGANATSAGEHTIALLLAITR
                     QIPHANESTHAGRWERKLIKPIDIRHKTVGIVGLGRVGSIVATRLKAFEMEVIAYDPY
                     ITAGRFDELGVEQVTLDELLGRADVVTFHVPSTDETHHLLRAETIARLKPGAIVINAA
                     RGEIVDQDALAEAIRSGHVSGAGVDVYPHEPARQSPLFGLPNVVLTPHTGGSSAEALL
                     AVGKVISSTTLAALRGEAVANAVNLPQSSIDAPVLRRLTTVAGAAGHLLSVLSSDTPE
                     LFRLTVTGMVPGDVIEHVLGSALSEAFQQWLGRRVTPVNARMVAEESGVRVATAIEDP
                     DETVLPRFVIEVRGATTHTVTITWDRVHSGIVEVDRFSLERPLAGHVLITHHHDQPGV
                     IGKLGTILGRYNVNIAGMQVGRHAPREEALMVTNVDDPVPDAAIAEIRGLDAVEDVFV
                     VALPPFDVDADPVVAGAMTAAVAMAGK"
     gene            2699..3085
                     /locus_tag="VFQ54_11640"
     CDS             2699..3085
                     /locus_tag="VFQ54_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0165687.1"
                     /translation="MENEQDTATSQRRVVVFSRDLFFGMRIRTTLRQLDYLTVLTKDL
                     PAFDAAMADPEFPPALGIIDFNQPVSWEFLSSIGSGTPIIAFGAHTDVDGFRAAKEAG
                     VARVISNGAFSKSLPDLLARYAFPVN"
BASE COUNT          542 a         1065 c         1064 g          562 t
ORIGIN      
        1 cagtcatgaa gaacctgcat cctctcgtgc atgcgacgcg tgtcatcggt atcgatttcg
       61 atggttatcg cagagatgga acgttccgtc cacgggagcg gtgaccgacg cgccgtgcag
      121 gtgcttccgg tggcgcgcta cactgcggga agcatcggca gcgtcgccgg tccccgcgaa
      181 acacccggcg atgggcaaga ggtcgttcgg gtgatcgccg gccggccaga ggcgacggtc
      241 cgagcatcac cctccccaat tgcaaccgat tcgaatcgcc tgatgggacg agacgctgaa
      301 cccatttcgc cgccagcgag gttatctgga ctcatgacaa acggacgcga acgacacgat
      361 atcggggcac ccctcccccg ctccttgcgc atcgccatcg atctcgacgg cgtgcttacc
      421 gaacatccgg cgccgctcgc ggcggccgcg aatgcccgct tcgacctcga cctgcccgag
      481 cgcgcgttca tcgactcggc gggcctgaac gtgccgctcg aggtccggga atgggtctac
      541 tccgacaccg gtcccgccgc gctcctcgag ccggccgcgg atgccgtgga gttcattcag
      601 cgggcgatcg atatcctcgg tgaaggaaac gcgctgatcc tgacggcgcg accggagaat
      661 tccgcggcga tgacggttgc ctggctgaag cgccacgggt ttccctccat caacgtgatc
      721 tttgccgacg acaagatgac cttcgcgcgt cgccagggat gcggcttcgc cgtcgaggac
      781 agtgagcgtc acgcccgcaa ctatgcagcc ggtggcgttc gctgctacct gatcgatccc
      841 gacggccaca tcgacgtcac tgatgagccg gagattgccc atgtcggcgg atacgcggaa
      901 atccttgccg agttgacggc acaagtcgag gccgccagat cccagcccgt ctcggtggtg
      961 aacacgctgc caccgatcgt cgacgcgttg accgcccggc cgcggatcgt cgtcagcgac
     1021 aacatccatc cggtcgcgcg acaggaactc gagtcgcagg ccgagatcgt cgacgtcgat
     1081 ggcaccgatg tcgacgccct gctggctgcc gtcgccgatg cggatgccct cgtcgttcgc
     1141 agcgagaccc aggtgaccga gcgcgtgcta ggtgccggtc gcaagctgcg agtcgtggcc
     1201 cgcgccggtg tcggtgtcga taacatcgat ctcggggcgg cgactcgcgc gggtgtgctg
     1261 gtcctgaacg ccccgggcgc caacgcgaca tcggctggcg agcacacgat cgcgctgctg
     1321 ctcgcgatca cgcggcagat cccgcatgcc aacgaatcga cgcacgcggg ccgctgggag
     1381 cgcaagctga tcaagccgat cgacatccgc cacaagacgg tcggaatcgt cggactcggt
     1441 cgcgtgggat cgatcgtggc gacgcggctc aaggcgttcg agatggaggt catcgcctac
     1501 gatccctaca tcaccgccgg acgcttcgat gaactcggcg tggagcaggt gacgctcgac
     1561 gaactcctgg ggcgtgccga tgtcgtgacc ttccacgtcc cttcgactga cgagacccat
     1621 cacctcctgc gagcggagac gatcgccagg ctgaagccgg gcgcaatcgt gatcaacgcc
     1681 gcccgaggcg agatcgtcga tcaggatgcc ctcgccgagg cgatccgctc cgggcacgtg
     1741 tcgggcgccg gcgtcgacgt ctaccctcac gagccggccc ggcagagtcc gctcttcggg
     1801 ctgccgaacg tcgtcctgac accgcacaca ggcggttcca gcgcggaggc actcctcgcc
     1861 gtcggcaagg tcatcagctc gacgacactg gcggcgctgc ggggtgaagc ggtggcgaac
     1921 gccgtcaatc tcccgcagtc gtcgatcgat gcaccggttc tgcgccgcct cactaccgtc
     1981 gcgggcgcgg ccgggcatct cctctcggtg ttgtcgtcgg acacgccgga gctttttcgg
     2041 ctcacggtca cggggatggt tcccggcgat gtcatcgagc atgtgctggg gtcggcgctg
     2101 agcgaggcgt tccagcaatg gctgggccgc cgtgtgacgc cggtgaatgc gcgaatggtt
     2161 gccgaggaat cgggtgtccg cgtcgcgacc gcgatcgagg atccggacga aaccgtcctg
     2221 ccgcgcttcg tgatcgaggt ccgcggcgcc acgacccaca cggtgacaat cacctgggac
     2281 cgcgtgcact cggggatcgt cgaggtcgac cgcttctcgc tggaacgacc gctggcgggc
     2341 cacgtgctga tcacgcatca ccacgatcag ccgggggtga tcggcaagct cgggacgatt
     2401 cttggtcgat acaacgtgaa catcgccggg atgcaggtgg ggcgtcacgc gccgcgtgaa
     2461 gaggcgctga tggtgaccaa cgtcgacgat ccggtgccgg atgccgcaat cgcggaaatc
     2521 cggggactgg acgcggtcga ggatgtcttc gtcgtggcgc tcccgccgtt tgatgtcgat
     2581 gccgatcccg tcgtcgctgg cgcgatgacc gccgccgtgg cgatggccgg aaaataggct
     2641 cacgggttgc ccggtgggga gatgcgctcc cgtcggcgct cgacaggatt gcatgacaat
     2701 ggagaacgag caggataccg ccacgtcgca gcggcgcgtc gtcgtcttta gtcgcgacct
     2761 ctttttcggg atgcggattc gcaccacgct caggcagctt gattacctca cggtgttgac
     2821 gaaggacctc cccgcctttg acgccgcgat ggccgatccc gagttcccgc cggcgcttgg
     2881 gatcatcgac ttcaaccaac cggtcagctg ggagttcctg tcgtcgatcg ggtctggcac
     2941 accgatcatc gcgttcggtg cccacaccga cgtcgacgga ttccgcgccg cgaaggaagc
     3001 cggggtcgcc cgagtgatct cgaatggcgc cttctcgaag tcgctgcccg accttcttgc
     3061 tcgatacgcg ttcccggtca actagtttgg tccaccgcct tgtgctgggg tgcgctctcc
     3121 tcgagtccgc ttacagagag gtataaaacc caacgatacc gcataaaccg atcggggcag
     3181 ggaacgggtc gcggtcggcg ctcccggtgc gcggttcgtt ctcgtcccgg cga
//
LOCUS       DASTMD010000784         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2394914, whole genome shotgun sequence.
ACCESSION   DASTMD010000784 DASTMD010000000
VERSION     DASTMD010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2394914"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFQ54_11645"
     CDS             complement(<1..891)
                     /locus_tag="VFQ54_11645"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEU0165688.1"
                     /translation="MNSVEHPRQPPVTMVNLAVRDLSSPVHLPRPLTPFVGRVAEVAA
                     IVARLRTPGVRLLNLIGPGGIGKTRLAIAVAGELAEDYPDGIHYVRLVAATDPVHVPL
                     AIAQSLELRETGRDLESRIADVIGYRRTLIVLDNFEQVVEAAPFVASLLGACHNLTIL
                     VTSRVRLQVTGENEYRVRPLHLPRLDQTSPPTIEELTSTDAVALFLDRARSTAAILDD
                     DDIRVVSEICDHLEGIPLGIELAAARLRVLSPRLVLDRLRHQLEFLTGGESDRPAHQR
                     TMRDTVAWSYDLLPPAEQSAF"
     gene            complement(1070..>2000)
                     /locus_tag="VFQ54_11650"
     CDS             complement(1070..>2000)
                     /locus_tag="VFQ54_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165689.1"
                     /translation="FAVSIPQDSRSGSVSWGQLYPGQVVFCDVYEILKAPSTAYGTVY
                     ISMHGCPAALTPTSDPNTLAQKCQAPMTGVTVFAVSRYSYYSDNRQGQINAASNYQFT
                     DVRAGDLTVSSAIPGTYGTPVIFCKAQIAGQTYGTVAYKQFPATTTGGYANIAWGPLL
                     QDQAVLCDWYQIPRSTAGGATDAASTVTVNVWQCASVVPNGSTVETFKAACTQAVTGV
                     TFSLTTGGTSRTSTTVGGKVEWTGLPNGAFSISRKTSPGGSGQAAFCGSTATAGGAII
                     SDPEVLVDLASGKVGGTIARARTQETCDWFDLP"
BASE COUNT          365 a          613 c          690 g          332 t
ORIGIN      
        1 gaaggccgac tgctccgccg gaggcaggag gtcataactc caggcgaccg tgtcgcgcat
       61 cgttcgctga tgcgcggggc gatcggactc gccaccggtc aggaattcga gctggtgacg
      121 aagccggtcg agcaccagcc gcggcgagag aacgcgcagc cgagcggcgg caagctcgat
      181 cccgagagga atgccctcga ggtgatcgca gatctcggag acgacacgga tatcgtcatc
      241 gtcgaggatc gccgccgtcg accgcgcccg gtcgagaaag agggcgaccg catccgtcga
      301 cgtgagctct tcgatcgtcg ggggcgaggt ctggtcgagc cgcgggagat gcagcggacg
      361 cacgcggtac tcgttctcgc cggtgacctg caagcggacc cggctggtga cgagtatcgt
      421 gagattgtga caggcgccaa gcagcgaggc aacgaagggc gcggcctcga cgacctgctc
      481 gaagttgtcg agcacgatca gagtgcgccg gtatccgatg acgtcggcga tcctcgattc
      541 gagatcgcgc cctgtctcgc gcaactcgag tgactgggcg atggcgaggg gaacgtgtac
      601 gggatcggta gccgcgacca ggcggacgta gtggatgccg tcgggataat cctcggccag
      661 ctccccggcg acggcgattg cgaggcgcgt cttcccgatc ccgccaggtc cgatgaggtt
      721 gaggagacgg acaccgggcg tccggaggcg ggcaacgatc gccgcgactt cggcgacgcg
      781 accgacgaac ggtgtcagcg ggcgcggcag gtggaccggc gacgaaagat cgcgcacggc
      841 cagattcacc atggtgacgg gtggctgccg tggatgttcg accgaattca tgaacgcgcc
      901 gatctcgaac gcagggtgat gacgttgcat caagtatatg agggacgaag accccgggcg
      961 tatgttcgtg gcttccgcca caggcagaag gcgacatctc gaaagatgcc gccttcatat
     1021 caacgaccag atccgcgttc cggatccgga tatcgcaacg acgccgggat caggggagat
     1081 cgaaccagtc gcaggtctcc tgtgtccttg cacgcgcgat ggtgccgccg accttgccgc
     1141 tcgccaggtc cacgagcact tcgggatcgg agatgatcgc gccaccggcg gtcgccgtcg
     1201 agccacagaa cgcagcctgt cctgaaccgc cgggcgacgt cttcctgctg atggagaacg
     1261 cgccgttcgg cagaccagtc cattcgacct tgccgccaac cgtcgtcgat gtccgcgacg
     1321 tcccgcctgt cgtgagtgag aaggtgacgc cggtcaccgc ctgcgtgcag gccgccttga
     1381 aagtttcgac cgtgctgcca ttgggcacga cgctcgcgca ctgccagacg ttgacggtga
     1441 cggtgctcgc cgcgtcggtt gccccgccgg cagtcgagcg cgggatctgg taccagtcac
     1501 agagcaccgc ctggtcctgg agcagcggac cccacgcgat attcgcgtaa cctccggttg
     1561 tggtcgcggg gaattgcttg tacgcaaccg tcccgtaggt ctgcccggca atctgcgcct
     1621 tgcagaagat gacaggcgtg ccgtacgttc cgggaattgc ggaactgacg gtcagatcgc
     1681 cggctcggac atcggtgaac tggtagttcg atgcggcatt gatctgcccc tgccggttat
     1741 cggagtagta gctgtagcgg ctgacagcga acaccgtcac gccggtcatc ggcgcctgac
     1801 atttctgggc gagcgtgttg ggatcggacg tcggggtcag ggccgccggg cagccgtgca
     1861 tgctgatgta gacggttcca taggccgtgc tcggcgcctt caggatctcg tacacgtcgc
     1921 agaagacgac ctgccccgga tagagctgcc cccaactcac gctgccgctc ctggagtcct
     1981 ggggaatgga gacggcgaag
//
LOCUS       DASTMD010000785         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_878207, whole genome shotgun sequence.
ACCESSION   DASTMD010000785 DASTMD010000000
VERSION     DASTMD010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878207"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="VFQ54_11655"
     CDS             complement(<1..1676)
                     /locus_tag="VFQ54_11655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0165690.1"
                     /translation="MTASDAAEQHEPVAPTPPAAVPPRLPDIEDIGGKEDEAFDPVRG
                     GLDRRIEIGASGWYGLLTVLGLLIAFGMRFSQLNIYSMAQGEAQWAYDSWALYLGKQL
                     PNGADLPTTSPLFLLLQSLSYFLFGVTDAIARIVPAIFGLLLVVLILLLRPFLSRFAI
                     VGMILLAAVSPTLVYTSRTSEPDILVGFCSFLFFVALLRAGAEGTKLTTTWMAVAGFA
                     FAGTLASGPDGVTALIAVLIGLGVAVVTDSSSASKTQGAVAASIRTVIANRRALASGV
                     AAAIVTIFVLFTRGFSDATALSGLGTTFSDWGKMMTTQSSNTPIPFFFYTTLLYEILA
                     VVFAIVAVTAGRSKDVESDSRHDLNATAFVTWFVVALLLESFASGKQPDQLSIVTLPL
                     VLLGGVGLGYMLERIPWSRMLNSTAGVVPIALAGILIGVIAALTLVARSNDPAQQNES
                     FLDIAMPIVFVLVIVVAPLIFLLAGETASRRQISHLGWSALLVVAVLLGLYTIGSSTS
                     LSFSRADTGLEPAAQGTSTSGLKAFVNQTLRLSRDMTDGNVSNIDNTGSYG"
     gene            complement(1751..>3098)
                     /locus_tag="VFQ54_11660"
     CDS             complement(1751..>3098)
                     /locus_tag="VFQ54_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165691.1"
                     /translation="LFTTMFTNLHGLATATYAPNGTLLYWLGQQSVRRGNQPWFYFIT
                     ESFQYEWLAIFLGTSALVVFGLRTARAALGRKVRPNLFFRIFLSFWFVFLFVVLSWAG
                     EKMPWLIMHFTLPAILLGSVLVEEVTSGAVGWLRTPRAARIRTISPRMTSFVLCIALV
                     VLAGAWFLIAAHLTAGTWVQDATGNWTRQIPHWAKSDWWMMAIPPIAAFALVGAAIWL
                     IGPRRAAYSVMVAALCVMALFEVHAGFRLAFLQGDTALDTMIYNTTSPDTTQLTHDVE
                     QMSQLIYGDNSMPVAFDGCTQWPLNWYMRNMPNRSIISDSTSSVQDNVPVIIGVPNGG
                     AGCGLPDEIPGYTSQTLTLRWHEPEYQIYRNFAIAPELTPGSSAWQSETQPHGLTDIA
                     SSIWSSLLTMTDPAGQQRDFELLMYRQMPAGENGYMFKVYIKNDMLPYYNDVRYGE"
BASE COUNT          621 a          952 c         1033 g          492 t
ORIGIN      
        1 ccgtagcttc cggtgttgtc gatattggag acgttcccgt cggtcatgtc ccgtgagagg
       61 cgcagcgtct ggttgacgaa tgccttgagg ccactcgtcg acgtcccctg ggcggctggt
      121 tcgagtccgg tgtcagcgcg ggagaaggag agcgacgtgc tgcttccgat cgtgtagagc
      181 ccaagcagga ctgccacaac gagcagtgcg gaccacccga gatgggatat ctgccgtcgc
      241 gatgccgtct cacccgccag caggaagatc aacggcgcga ccacgatcac gaggacgaag
      301 acgatcggca tcgcgatgtc gaggaagctc tcgttctgct gggccggatc gttcgagcgg
      361 gcaacgaggg tcagggccgc gatcacgccg atcaggatgc cggcgagcgc gatcggaacc
      421 actccggccg tcgagttgag catgcgactc caggggatgc gctcgagcat gtagccgaga
      481 cccaccccac cgagcagcac cagcggcagg gtcacgatcg agagctggtc cggttgcttg
      541 cccgaggcga agctctcgag cagcaacgcg accacgaacc acgtcacgaa cgccgtcgcg
      601 ttgagatcgt ggcgggaatc agactcgaca tccttcgaac gccccgcggt gacggcgacg
      661 atcgcgaaga cgaccgcgag gatctcatac agcagggtcg tgtagaagaa gaacgggatc
      721 ggggtgttcg atgactgcgt cgtcatcatc ttgccccaat ccgagaaggt cgttcccaga
      781 ccggaaagcg cggtcgcatc cgagaatccg cgggtaaaga gcacgaagat cgtgacgatc
      841 gccgccgcaa caccggaggc cagcgcccgc ctgttcgcga tgaccgtccg gatcgacgcc
      901 gcgaccgccc cctgcgtctt cgatgctgac gaggaatcgg tcaccaccgc gacgccgaga
      961 ccgatcagca cggcaatcaa tgcggtcacg ccatccggtc cgctcgcgag cgttcccgcg
     1021 aaggcgaaac cggcgacggc catccacgtc gtcgtgagct tcgtcccttc ggcgccggcg
     1081 cgtagcaggg cgacgaagaa gaggaaggag cagaagccaa ccaggatatc cggctccgag
     1141 gtgcgggagg tgtagaccag ggtcggtgag accgccgcca gcaggatcat cccgacgatc
     1201 gcgaaccggg agaggaacgg tcgcaggagc aggatcagca cgacgaggag caatccgaag
     1261 atcgccggga cgatccgcgc gatcgcatcg gtgacaccga acaggaaata cgagagggac
     1321 tggagcagca ggaagagtgg cgatgtcgtc gggagatcgg caccattcgg cagctgcttg
     1381 ccgagataga gcgcccacga atcgtaggcc cactgtgcct cgccctgcgc catcgagtag
     1441 atattcagtt gcgagaagcg catcccgaag gcgatcagga ggccgagcac ggtcagcaga
     1501 ccgtaccagc cgctcgcgcc gatctcgatc cggcggtcga ggcccccgcg cacggggtcg
     1561 aatgcttcgt cctctttccc gccaatatcc tcgatatcgg gaagtcgcgg gggaaccgcc
     1621 gccggtggcg tcggcgcaac tggctcgtgt tgttcagccg cgtcagatgc ggtcacgctc
     1681 tcctcctgat gttccgttgg ctgggaattg gattggtcgg ggcccgtcgg gcgctggtcg
     1741 cgaggatcgc tcattcgccg taccgcacgt cgttgtagta cggcagcatg tcgttcttga
     1801 tgtagacctt gaacatgtag ccgttctcac cggccggcat ctggcgatac atgagcaact
     1861 cgaaatcacg ctgctgaccg gcgggatcgg tcatcgtcag gagactgctc cagatcgaac
     1921 tcgcgatgtc ggtcagaccg tgcggttggg tctcggactg ccacgcggaa ctgcccggcg
     1981 tgagttcggg tgcgatcgcg aagttgcgat agatctggta ttcgggctcg tgccagcgga
     2041 gggtcagcgt ctgcgacgtg tatcccggga tctcgtccgg caggccgcaa ccggcgcccc
     2101 cattcggcac gccgatgatc accggcacgt tgtcctggac cgaggatgtc gagtcgctga
     2161 tgatcgatcg gttcggcatg ttccgcatgt accagttcag cggccactgc gtgcagccgt
     2221 cgaaggccac gggcatcgag ttgtcgccat agatcagctg cgacatctgc tcgacgtcgt
     2281 gcgtgagctg ggtcgtgtcg ggtgaggtgg tgttgtagat catggtgtcg agcgccgtat
     2341 ctccctggag gaacgcgagc cggaacccgg cgtggacctc gaaaagcgcc atcacgcaga
     2401 gcgcggcgac catcacggag taggcagccc gccgcgggcc gatcagccag atcgccgcgc
     2461 cgaccagcgc gaaggcagcg atcggcggga tcgccatcat ccaccagtcc gacttggccc
     2521 aatgcggaat ctgacgcgtc cagttgccgg tcgcgtcctg gacccaggtg cccgccgtca
     2581 ggtgggcggc gatcaggaac caggctccgg caagcaccac gagcgcgatg cagagcacga
     2641 acgaggtcat gcgcggcgag atcgtcctga tccgcgccgc acgcggcgtc cgcaaccagc
     2701 ccaccgcgcc actcgtcact tcctcgacca gcaccgaccc gagcaggatc gccggcaggg
     2761 tgaagtgcat gatcagccag ggcatcttct caccggccca ggagaggacc acgaagagaa
     2821 agacgaacca gaaggaaagg aagatccgga agaagagatt gggccgaacc tttctcccga
     2881 gcgcggccct tgccgtccgc aatccgaaga cgacgagggc cgatgtgccg aggaagatcg
     2941 ccagccattc gtactggaat gattccgtga tgaagtagaa ccacggctgg ttgccacggc
     3001 gcacgctctg ctggcccagc cagtagagca gcgtgccgtt gggggcatag gtcgccgtgg
     3061 cgagaccatg cagattggtg aacatcgtgg taaagagc
//
LOCUS       DASTMD010000786         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2751916, whole genome shotgun sequence.
ACCESSION   DASTMD010000786 DASTMD010000000
VERSION     DASTMD010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2751916"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="VFQ54_11665"
     CDS             complement(<1..1422)
                     /locus_tag="VFQ54_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU0165692.1"
                     /translation="MPLTSRVSRRTDLRNIAIIAHVDHGKTTLVDGLLKQSHIFRDPD
                     AAGTLIMDSNDIERERGITILAKNTAIAYHDVKINILDTPGHADFGGEVERVLNMADG
                     CLLLVDAAEGPMPQTRTVLGQALQLGLRPIVVINKIDRSDARIDEVIELVNDLFLDLA
                     IDDAQLEFPILYAIGRAGRAGFTPDTLADDLIPLLDTILNTVPAPEVELDGPFQMLVT
                     SLDYDSHRGRIAIGRIHRGKVKMGDLVALVDEQLGATPRLRVSSLMTFVGLAKKEIEE
                     ATAGELVALTGFADARIGATLTDPEAPEALPAVAIEEPTLKLTFGVSTSPFSGRDGQY
                     STSRQLGDRLRRELETNLSLRVESTEQADVFSVAGRGELHLSVLIETMRREGYEFQVS
                     RPEVITRRDDTGAVYEPVEHLVINVPEEFVGGVTELTGSRKARMTDMVNDGRGNVRLE
                     FSIPTRGLIGLRNAVLTATKGNGL"
BASE COUNT          414 a          708 c          731 g          385 t
ORIGIN      
        1 caggccgtta cccttggtcg ccgtcagcac cgcattgcga agaccgatca ggccgcgggt
       61 cgggatcgag aactcgagac ggacatttcc ccgaccatca ttgaccatgt cggtcatgcg
      121 ggccttgcgg gaaccggtca actcggtcac gccaccgacg aactcctcgg ggacattgat
      181 cacgagatgc tcgaccggct cgtacaccgc gccggtgtca tcgcgacggg tgatgacttc
      241 cggtcgggag acctggaatt cgtagccttc gcggcgcatc gtctcgatca ggaccgagag
      301 gtgaagctca ccgcgaccgg ccaccgagaa gacatcggcc tgctcggtcg actcgacgcg
      361 aagggagagg ttcgtctcca gttcgcgacg caggcgatcg cccagctggc gggaggtcga
      421 atactggcca tcgcgacccg agaacggcga ggtgctgaca ccgaaggtca gcttcaacgt
      481 cggctcttcg atcgcgacgg cgggaagcgc ctccggcgcc tcgggatccg tgagcgtggc
      541 gccgatccgc gcgtccgcga aaccggtcag cgcgaccagt tcaccggcag tggcctcttc
      601 gatctccttc ttcgcgagac cgacgaaggt catcagcgag gagacgcgca ggcgcggggt
      661 cgcgccgagc tgctcgtcga cgagggcgac gagatcgccc atcttcacct tgccgcgatg
      721 gatgcgaccg atcgcgatgc gaccgcggtg ggagtcgtaa tcgagcgagg tcaccagcat
      781 ctggaacgga ccgtccagct cgacttccgg cgccggcacg gtgttgagaa tcgtgtcgag
      841 cagcgggatc aggtcatcgg cgagggtgtc cggcgtgaag ccggcgcgac cggcgcggcc
      901 gatcgcgtag aggatcggga actcgagctg ggcatcgtcg atcgcgagat cgaggaagag
      961 atcgttgacg agctcgatga cctcatcgat ccgggcatcc gagcggtcga tcttgttgat
     1021 gacgacgatc gggcggagac cgagctgcaa cgcctgcccg agtacggtcc gcgtctgcgg
     1081 catcggaccc tcggcggcat caacgaggag gaggcaaccg tccgccatgt tgaggacgcg
     1141 ctcgacctca ccaccgaagt cggcgtgacc gggggtatcg aggatgttga tcttcacatc
     1201 gtggtaggcg atggcggtgt tcttggcaag aatggtgatg ccgcgctcac gctcgatatc
     1261 gttcgaatcc atgatcagcg tcccggcggc gtccggatcg cggaagatat gcgactgctt
     1321 gagcagaccg tcaacgagcg tggtcttgcc gtggtcgacg tgggcaatga tggcaatgtt
     1381 tcggaggtcg gtccggcgcg aaacgcgtga ggtcagaggc atggatggaa aactcgattc
     1441 atgaatgagg gttcgatgtc gcatccattc aggaccgggg cgatcattcg cgatccgggc
     1501 gcgcaacaga ttgcgggtgt gcaatcttga acgacatcaa cctggattgt attcgagacg
     1561 aaggacacta taaggggaca cacgagaaga cccgtgccgt tgcaacacgg gccattcagc
     1621 gctcaccgct gcttacttaa gaatatcaca tcgcccggaa tcagccgtta tggtgcgatt
     1681 atcgacgccc cgactcgacg ccagccagca ggacgcagga gatcacgccc tccaaagaga
     1741 gggcgggaca ttcctccctc caaagcccgg gcatccaccg ggcgaggaat cccgctcgcc
     1801 ggtcttcggg ctccgcagtc caatgccgcc ccaagcacca acgctgtttc attgacctgg
     1861 tgggcaccga cctggtgtgg ctcgcagccc tgcgtgttcc gtccccacgg cgcttgtcgc
     1921 cgttccgggc cggcgccgtc gaccctttct cgtcatcgac gcggccccgt ggctgcctcg
     1981 gtcagtgcgg cgcctccccg gggacgtcgc gcgggccagc accaggcggg acattcctcc
     2041 gtccagcgac caaagatcga gcggccatcg ctcctgaggc aggtcgctgc actccgtagt
     2101 cccgcccttg tatgtcatga tcgtggggtg gcgccgtcct ccgatcccgc ccggggtctt
     2161 ccacgaccgt tgcgtagtcc ggagacggcg gccacaccga ccccgaccag gactcccacg
     2221 aacgggtacc agcgcttt
//
LOCUS       DASTMD010000787         3163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_786614, whole genome shotgun sequence.
ACCESSION   DASTMD010000787 DASTMD010000000
VERSION     DASTMD010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786614"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1160)
                     /locus_tag="VFQ54_11670"
     CDS             complement(312..1160)
                     /locus_tag="VFQ54_11670"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEU0165693.1"
                     /translation="MKVYVSVDIEGISGVVHADMMMPGQYEYARGRALMIGDANAAVE
                     GAVQAGADYILVSDGHGPMRNLQIEHLHPAAHLVTGTADAKDYCQLEGAETETFDAAV
                     FVGYHAMAKTFKAIHPHTIAGACVYELRVNGRPHGETGLNAFVLASLGIPTVAVTGDV
                     TTCGEAKSFLGDNIETVAVKSATGRNAAICRPPAATAPEITEAVKRALGNISAVPLYK
                     AEGPATIEIDFVTMVQCERAHRTQGVERVGPMTTSILGETPWEQYKNAWAAIRSALYE
                     PASFLA"
     gene            1320..2015
                     /locus_tag="VFQ54_11675"
     CDS             1320..2015
                     /locus_tag="VFQ54_11675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0165694.1"
                     /translation="MSISVQITAPTPAFIFDLDGTLIDSLYPHVLAWKQAFEDDGMEV
                     SGWRIHRRIGMSGDLFVRAIFRELDRPIDDATTERIQKRHGDLFQDVKDQVRPIPGAR
                     ELLHALTEAGAPWAIATSGHIRSASHGLAMLGILDGPAPIVTRDLVKFAKPDPDLFLA
                     AAQKLDVPIERAFVIGDSVWDMLAAGRVHALGIGVRCGGNGEDELKAAGAYRIFDDPA
                     EILAHLDELGVHR"
     gene            2187..2633
                     /locus_tag="VFQ54_11680"
     CDS             2187..2633
                     /locus_tag="VFQ54_11680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU0165695.1"
                     /translation="MSDPLAQLRPLDKKRTILLTSYRKDGTPVGTPVNIAIDNNDHAV
                     IRTWTTTGKYKRLMRNPEVEVAPSTVRGNPTGPAVHAHVRLLEGNESEDAAKALARKY
                     PIIQGVLVPRVHKLRGLHTVHFELLPDEGEARGASGDENPAPLDEA"
     gene            complement(2699..>3163)
                     /locus_tag="VFQ54_11685"
     CDS             complement(2699..>3163)
                     /locus_tag="VFQ54_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165696.1"
                     /translation="YVMGGLALFVLAGSASVTVFLVRSVPSATVMNFGIVAVLIGQIV
                     TLVGIEASSIVTFFVGGVISGAGFGAGFQGAVRMVLPLAEPHQRAGVLSMVYVVCYLA
                     MGLPAVIAGYRVVHSDVVTTGEEYGVAVIVLVVIAAIGQIATQVRTRARAIA"
BASE COUNT          589 a         1086 c          970 g          518 t
ORIGIN      
        1 gcgcatctgc ggcacgatct catctccggg gagtgggatc gcgagtatgg ccatctccga
       61 tcgcagccgt cgtacgatgg ctcgctccgc ctgatcgtgg accgcaccgg cgagaagcgc
      121 cgctggtcga tgatcgggcg ctgatctcgt cgcccgttcc gggacggaaa gctcccgtga
      181 gaccccgcca tccgcacgag tgcccgtccg tgacgcggcc gtgcggacgg gggggacttc
      241 tctgtccccc cttgaatgcg cgcgattcgg tcccgtcgag cacgaggtca ttgagctcgc
      301 gccataactg cctacgccag gaacgacgcc ggctcgtaga gcgccgagcg gatcgccgcc
      361 caggcattct tgtactgctc ccacggcgtc tcaccaagga tcgaagtcgt catcggtccg
      421 acccgctcca caccctgcgt gcgatgggcg cgctcgcact gcaccatcgt cacgaagtcg
      481 atctcgatcg ttgccggtcc ctccgccttg tacagcggca cggcggagat attgccgagt
      541 gcgcgcttca ccgcctcggt gatctccggc gccgttgcgg ccggcgggcg gcagatcgcc
      601 gcgttgcgac ctgtcgccga cttcaccgcg accgtctcga tgttgtcacc gaggaacgac
      661 ttcgcctcgc cgcaggtcgt gacatcgccg gtcacggcaa cggtcgggat gccgagcgac
      721 gcgaggacga aggcgttgag accggtctcg ccatgggggc gaccattcac gcgaagttcg
      781 tagacgcagg cgcccgcgat cgtgtgcggg tgaatcgcct tgaacgtctt cgccatcgcg
      841 tgatacccga cgaagaccgc cgcatcgaag gtctcggttt cggcaccctc gagctggcag
      901 tagtccttgg cgtccgccgt tcccgtcacg agatgcgccg ccggatgcag atgctcgatc
      961 tgcaggtttc gcatcgggcc gtggccatcg gagaccagga tgtagtcggc gccggcctgg
     1021 accgcgccct cgaccgcggc attcgcgtcg ccgatcatca gcgcacggcc acgcgcatac
     1081 tcgtactgtc ccggcatcat catgtccgcg tggacgacgc cggaaatccc ctcgatatcg
     1141 accgagacat agaccttcat cgcttgccct tccatccatc atgttcggag acacgtttgt
     1201 ctcgagaaac ctcgcgcgat catacggtgc atttccccgg ccgggaacac gctgcgtcaa
     1261 ttcacgaaac agcggacaat accaggatcg tcgcgttctc cccaaccatg aggacatcca
     1321 tgagtatcag cgtacaaatc acggcgccaa ccccggcttt catcttcgat ctcgatggaa
     1381 cgttgatcga cagcctctac ccgcacgtcc tcgcctggaa acaggcattc gaggatgatg
     1441 ggatggaggt ctcgggatgg cgcattcatc gcaggatcgg gatgagtggc gatctcttcg
     1501 tgcgcgccat cttccgggaa ctcgatcgac cgatcgacga cgccaccacc gagcgcatcc
     1561 agaagcggca cggcgatctc tttcaggacg tcaaggatca ggtccgtccg attcccggcg
     1621 cccgcgaact gctgcacgcg ttgacggagg ctggtgcacc gtgggcgatc gcgacgagcg
     1681 gccacatccg cagcgcaagc cacggcctgg cgatgctggg gattctcgac ggcccggcgc
     1741 cgatcgtcac tcgcgatctc gtcaagttcg cgaaaccgga tcccgatctc ttcctcgccg
     1801 ccgcccagaa gctcgacgtg ccgatcgagc gcgccttcgt gatcggtgat tctgtctggg
     1861 atatgttggc cgcgggtcgc gtccacgccc tcggcatcgg cgtccgctgc ggcggcaacg
     1921 gggaagacga gctgaaggcg gcaggcgcct atcgcatctt cgacgatccc gccgagatcc
     1981 tcgcccacct cgacgaactc ggcgtccatc gctagatctc gccggcgcga ggatgacatg
     2041 aacgtcggca tcgatcggcg gcggcgcgct tcctgcggaa gttcgcgaaa agcaccgcca
     2101 tccgcatcga gcgtggcggc atgcgccgcg ccgcgcccct cgttgatcga cccgcgtggg
     2161 cgcgacgatt cgaagggagg atctcgatga gcgacccact cgcgcagctc cgtccgctgg
     2221 acaagaagcg gaccattctg ctcaccagtt accgcaagga cgggacgcca gtcggaacgc
     2281 cggtcaacat cgcgatcgac aacaacgatc atgcggtgat ccggacgtgg acaacgacgg
     2341 gcaagtacaa gcgactgatg cgcaatcccg aggtcgaggt ggcaccctcg accgtccgcg
     2401 ggaatccgac gggacccgcc gttcacgccc acgtccgcct gctcgaagga aacgaatcgg
     2461 aggatgccgc gaaggcgctc gcgcgcaaat acccgatcat ccagggcgtc ctcgttcccc
     2521 gggtgcacaa gctgcgcggc cttcacaccg tccacttcga actattgccc gatgagggcg
     2581 aagcgcgagg cgcatctggc gacgagaatc cggctcctct agacgaggca tagaaaggcg
     2641 cccggccgat ccgcatggac cagcagggcg ccgaatgagt ctgaggtcaa tcagatcgtc
     2701 aggcgatggc gcgggcccgc gtccgcacct gcgtcgcgat ctgaccgatc gccgcgatca
     2761 cgacgagaac gatgacggca acgccgtact cctcgccggt ggtcacgaca tcactgtgca
     2821 ccacgcggta gcccgcgatc accgccggca atcccatcgc gagatagcag accacgtaga
     2881 ccatcgaaag gacgcctgcc cgctggtgtg gttccgccag cggcaacacc atccgcacag
     2941 cgccctggaa gccggcgccg aatcccgcgc cggagatcac gccgccaacg aagaaggtga
     3001 cgatcgatga cgcttcgatc cccaccagcg tcacaatctg cccgatcaac acggcgacga
     3061 tcccgaagtt catcaccgtc gccgatggca ccgatctgac gaggaagacc gtgacgctcg
     3121 cgcttccggc gagcacgaag agggcaagcc cgcccatcac gta
//
LOCUS       DASTMD010000788         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_697257, whole genome shotgun sequence.
ACCESSION   DASTMD010000788 DASTMD010000000
VERSION     DASTMD010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697257"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..380)
                     /locus_tag="VFQ54_11690"
     CDS             complement(144..380)
                     /locus_tag="VFQ54_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165697.1"
                     /translation="MTTRVYLAAVKFVDGSGQPGDLPAERVFVNASDVPEVWVETEST
                     VVGDVGRRVAFLIARPMDIGFSRVTGTIERKLEK"
     gene            complement(468..1496)
                     /locus_tag="VFQ54_11695"
     CDS             complement(468..1496)
                     /locus_tag="VFQ54_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165698.1"
                     /translation="MWDPEQYEDTDYGLEPGESFEPDDAWGDESADLGQPHVMTVLGP
                     IDPDELGIALHHEHLLCNPTAVTGETPDFLLDNPENAIADLEAYVSVGGRGILDASSR
                     DYGRDVDGLVGLAQRVPVHIITVTGRHKDLHASRMANADDVDALVEEYRAEIRDGVGQ
                     FRVRPGAVKFGTSLDEMTPVEGYAVRAAARISIETGIPITTHTEVGTHALVQLETLAS
                     EGADLRRVIVGHLDRKMEWDYLIEILRSGAFVSFDNVGKAFYGPDGPKATTLVRLAEA
                     GYADQLLISQDIARRSGYVSYGGAPGITHLLERFTLTLVDAGAEALLVRQMLVENAAR
                     ALTTIPPP"
     gene            1659..2894
                     /locus_tag="VFQ54_11700"
     CDS             1659..2894
                     /locus_tag="VFQ54_11700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165699.1"
                     /translation="MTGFRSFLRTPVGYGTILLATATFFVGFAMAAQQNIVSNYFENE
                     IGLKGPEFGYITAIREVPGFLLIFVAALFYRLSLARLTALALLVLTIGYGFFGFSSSF
                     WTVAPWVIISSVGYHTWLQTQSALGLSLTTEKRAGWILGRVSAVNQAGTMIAMALVLL
                     TFEFNILSYRGTFILCGLAALIGAIAIFRFPHLHNGKVLEVKASRPPIVLKRDYRLYY
                     ALSVLDGARQQIFFSFGLWVLVNTYHLTVPQISVVLLVVAVIAIVAGPRIGKLIDLYG
                     EKTILGFVNIGYVIALLGYALASNASFAVSFYVIYTFIAPLSAMGATVYLRKIATASD
                     LAPSLAMGLTMQHAAAIVVPVVTGFILNYVGYQIPFLVGGCFAILGLFLTRRLDSDNQ
                     KSEGRRAEELRLSTAAEAA"
BASE COUNT          500 a          965 c          855 g          578 t
ORIGIN      
        1 tccggcaacc cgcagtgaaa tccacgcacg tcgacatcgt ttgcgccgcc ggggttggta
       61 gggcggggtt ttataccccg ccgcaaagcg gattcgggcg aagcacgccg tgcttcaggg
      121 tagctaccga tgcaggtttg gctctatttc tccagcttgc gctcgatcgt acctgtaaca
      181 cgagagaagc cgatatccat cgggcgggca atcagaaaag ccacccgtct gcccacatcg
      241 cctaccacgg tgctctcggt ttcgacccag acttcgggaa cgtcagacgc attcacaaat
      301 acccgctccg ccggcaaatc acccggctga cccgacccgt ccacaaactt caccgccgcc
      361 agatacaccc gggtcgtcat cgctcctcct ccgacaccat gtctcagtga ctactaactt
      421 cgcatgatac agagaatgtg atgctccgcg agcgcgaaag cgtcaattta cgggggtggt
      481 atcgttgtca gcgccctcgc cgcgttctcg accagcatct ggcggacgag aagcgcctcg
      541 gcgcccgcat ccaccagcgt cagcgtgaag cgctccagca gatgggtgat ccccggcgcg
      601 ccgccgtagg aaacgtagcc cgaccgccgc gcgatgtcct gcgagatcag gagctggtcg
      661 gcgtatcccg cctccgcgag ccggacgagc gtcgtcgcct tcggcccgtc aggaccgtag
      721 aatgccttgc cgacattgtc gaatgagacg aacgcgcccg accgcaggat ttcgatcaga
      781 tagtcccact ccatcttccg atcgagatgg cccacgatca cgcggcgaag atcggcaccc
      841 tcgctcgcca gcgtctcgag ctggacgagc gcgtgcgttc caacctcggt gtgcgtcgtg
      901 atcggaatcc cggtctcgat cgagatccgg gccgccgcac ggacggcgta tccctcgacc
      961 ggcgtcatct cgtcgagact cgttccgaac ttgacggccc cgggtcgcac gcggaactga
     1021 ccgacaccgt cccgaatctc ggcccggtat tcctcgacca acgcgtccac gtcatccgca
     1081 ttcgccatcc gcgacgcgtg gaggtccttg tggcgaccgg tcacggtgat gatgtgaacg
     1141 ggaacccgtt gtgccaaccc cacgagtcca tcgacatcgc gtccgtagtc tcgcgatgag
     1201 gcatcgagga tgccgcgtcc gccaaccgaa acatatgcct cgagatcggc gatcgcgttc
     1261 tcggggttgt cgaggagaaa atccggcgtt tcgcccgtga ccgccgtcgg gttgcagagc
     1321 aggtgctcgt ggtggagggc gataccgagt tcgtcgggat cgatgggtcc gaggaccgtc
     1381 atcacatgcg gctggccgag gtcggccgac tcatcgcccc aggcatcgtc cggctcgaac
     1441 gattcgccgg gctcgagccc atagtccgta tcctcgtact gttccggatc ccacatcggt
     1501 cgatcgtcct tcctctccgt cgagactcgc tccggggcga gcagtctgat gcccggcatt
     1561 atcgcttgcg cgggcgaccg cgtcgtgaga gcgtgggata atgcagcacg ctccagacga
     1621 ggcgaagtga tggaaaaggg tgagaagagg tggaagggat gacgggcttt cgctcgtttc
     1681 tgaggacgcc ggttggctac ggcacgatac tcctggcgac ggcgacgttc ttcgtcggat
     1741 tcgcgatggc ggcgcaacag aacatcgtct cgaactactt cgagaacgag atcggcctga
     1801 agggcccgga gttcggatac atcacggcga tccgcgaggt tccgggcttc ctgctgatct
     1861 tcgtcgccgc gctcttctac cgtctctccc tggcgcggct gaccgcgctc gccctgctcg
     1921 tcctcacgat cggatacggc ttcttcggct tctcgtcctc cttctggacg gtcgcgccgt
     1981 gggtgatcat cagcagcgtc ggctatcaca cctggctgca gacgcaatcc gcgctcggcc
     2041 tctcactcac gacggagaag cgcgccggct ggatcctcgg tcgcgtcagt gccgtcaatc
     2101 aggcgggcac gatgatcgcg atggcgctcg tgctgctcac cttcgagttc aacatcctct
     2161 cctaccgcgg gacgttcatc ctctgtgggc tcgccgcgct gatcggcgcg atcgcgatct
     2221 tccgctttcc gcatcttcac aacggcaagg tgctggaagt caaggcctca cggccgccga
     2281 tcgtgctcaa gcgcgattac cgcctctact acgccctcag cgttctcgac ggagcacggc
     2341 agcagatctt cttctcgttc ggcctgtggg tgctggtcaa tacctaccat ctcacggtgc
     2401 cccagatttc ggtggttctc ctggtggtgg cggtgatcgc gatcgtcgcg ggaccgcgaa
     2461 ttggaaagct gatcgatctt tacggtgaga agacgattct tggcttcgtc aatatcggct
     2521 atgtgatcgc gctgctcggc tacgcactcg cgagcaacgc ctcctttgcc gtttcattct
     2581 atgtcatcta caccttcatc gctccgctct cggcgatggg tgcgacggtc tatctccgca
     2641 agatcgcgac ggcctccgat ctcgcgccat cgctcgcgat gggcctgacg atgcaacacg
     2701 cggcggcgat cgtcgtcccc gtcgtgacgg gtttcatcct caactacgtc ggctatcaga
     2761 tccccttcct cgttggagga tgcttcgcaa tcctcgggct cttcctgacc cgccgcctcg
     2821 actctgacaa ccagaagagc gaggggcggc gagccgagga acttcgcctc agcaccgccg
     2881 ccgaggccgc ctgactgc
//
LOCUS       DASTMD010000789         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1591958, whole genome shotgun sequence.
ACCESSION   DASTMD010000789 DASTMD010000000
VERSION     DASTMD010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591958"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFQ54_11705"
     CDS             <1..238
                     /locus_tag="VFQ54_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165700.1"
                     /translation="QELFDEFNVGWEQELVTTHAEVTDGRLQIPIGPGLGTDLNWSEL
                     NKHPYQASNFLPLFAPGWERREGERPAGSPDPNA"
     gene            462..1019
                     /locus_tag="VFQ54_11710"
     CDS             462..1019
                     /locus_tag="VFQ54_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165701.1"
                     /translation="MDDEDILIAAALVARLLDEIPACDTRPVRASHWGSDRAAARLWA
                     SLGFVRLMRTRLATLDAGMAISEPEILPTGFSILTGAELPDDGELWPRIAMLHAEIYQ
                     RNHQWSPPREIGKDLAMALFLDPDDLIRDALFVLIAEGSPVGVASLRRGGSANRCEMG
                     WIGVELGTAALRDLAIDVLLKECLD"
     gene            complement(1179..2297)
                     /locus_tag="VFQ54_11715"
     CDS             complement(1179..2297)
                     /locus_tag="VFQ54_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0165702.1"
                     /translation="MKRQATIDDIAAASGCSPATVSLALRNKPGVSRATRERILSTAR
                     ALGYQRVQSMPTADRQTLDVAIVFRTWGYERANSSPNIVDFYSWVLTGLQESAGGQGI
                     NLLLANIPINSRNEATEYPERLLRQPLDGVILVGSFRPEVIAQAESIASTHRPPMILV
                     DCGLEHHDMDSVDTANWEGGYQATRHLIANGHRKIAYLGPVSTWEPSFRNRREGYLDA
                     LRDAGLTTAGLFEESPEQMLARDAGRTALNENDGATAFFCANDDTATALMRNAASMGI
                     RIPADLSVLGFDDIIRGREAIPPLTTMGVDKHGMGRHAMYILGQRTSRPESVPIQIML
                     RPRLVERESVRRLDVAVEAATDPGAPADVEPEPVVIAE"
     gene            complement(2492..>3246)
                     /locus_tag="VFQ54_11720"
     CDS             complement(2492..>3246)
                     /locus_tag="VFQ54_11720"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEU0165703.1"
                     /translation="SHHAGNINGVPTDEPEGTCLAVSPRIFKFHGRTAHAALDPHNGI
                     NALNAVIHLFTGIDALRQHVTPDVRIHGIITKGGVAPNVVPDYAEAYFMFRGATRGKV
                     DEIVEKAVKIAEGAALMTGARLEIDASQPDYDDVLPNYTLGRLLQEKLEPAGLAETPV
                     DQKPRQTGPAPYSTDLGNVSRKMPTSAVNFAISTVPIPGHSIEVVEGSISDLGRANAI
                     ATGKALALAAADLLMNPELYARAKAEFAAAAE"
BASE COUNT          550 a          975 c         1100 g          621 t
ORIGIN      
        1 gcaggaactc ttcgacgagt tcaatgtcgg gtgggaacag gaactcgtga cgacccatgc
       61 cgaagtgacc gatggtcgcc tccagattcc gatcggaccc gggctgggga ccgacctcaa
      121 ctggtcggag ctgaacaagc acccctacca ggcgtcgaac ttcctgccgc ttttcgcgcc
      181 aggatgggag cgccgcgaag gtgagcgacc cgccggaagc cctgacccca acgcgtaatc
      241 cggtgggtga tctccggatt cggcccgcgc gacctgacga tctctggcac gagcctgtcc
      301 ctgcgaccgg ggaaaggctc gtcgtgttcc ccgactcgga cgaccgcgat cgatttcgtc
      361 ggacgctggc cgccgagcga gatgggcgga tcgttgccgt cggatccatc cacacggaat
      421 ggctccatcc ctcgcacgtt cggatatcca tcgaaccggt cgtggatgac gaagacatcc
      481 tgattgcggc cgcgctcgtg gcgcgactgc tcgacgagat ccccgcgtgt gacacccggc
      541 cagtccgggc gagccattgg ggatcggatc gagcagcggc acggctctgg gcatcgctgg
      601 gctttgtgcg tctgatgcgc acccggctgg cgacgctcga tgcgggcatg gccatttccg
      661 agccggagat cctccccacg ggattctcga tcctgaccgg tgccgaactc ccggacgacg
      721 gtgagttgtg gccgcgcatt gccatgcttc atgcggagat ttatcagcgc aatcaccaat
      781 ggagcccgcc aagagagatc gggaaagacc tggcgatggc gctcttcctc gatcccgacg
      841 acctgatccg cgatgcgctt tttgtactca tcgcagaggg atcgccggtt ggtgtggcca
      901 gtcttcgccg tggagggtcc gccaatcggt gcgagatggg ctggatcggt gtcgagctgg
      961 ggacggccgc attgcgggat cttgcgatcg acgtactgct gaaggaatgc ctcgactaag
     1021 cgatcgaacg tgcatggtcg atcgacgtcg aggcggacga tgcggatccg atcctgtcgg
     1081 cgtggctgga cctgatgccc gttcccgatc tccgggagtg gatcgccgtg gagaagacgc
     1141 gatcgcagga agcaggctga accgggaggc gagggccctt actcggcaat gacgacgggt
     1201 tcgggctcga cgtcggcggg agccccggga tcggtcgccg cctcaacggc aacgtcgaga
     1261 cggcggacgg attcgcgctc gacaaggcgc ggtcgcagca tgatctgaat cgggaccgat
     1321 tccggtcgcg acgtccgttg gcccaggatg tacatagcat ggcgacccat gccgtgcttg
     1381 tcgaccccca ttgtcgtcag tggcggtatc gcctcgcggc cgcggatgat gtcatcgaag
     1441 ccgaggaccg agaggtcggc ggggatgcga atgcccatgc tggcggcgtt tcgcatgagc
     1501 gccgtggccg tgtcgtcgtt ggcgcagaag aaggccgtcg caccgtcgtt ctcgttcagg
     1561 gcggttcgcc cggcgtcccg cgccaacatc tgctcgggcg attcctcgaa gagtccggct
     1621 gttgtcaggc cagcatcgcg gagggcatcc agatatccct cgcgacgatt tcggaagctt
     1681 ggctcccagg tactgacggg accgagatag gcgatcttgc ggtgtccgtt tgcaatcagg
     1741 tgacgcgttg cctggtagcc gccctcccaa ttcgcggtat cgacgctgtc catatcgtga
     1801 tgctcgagac cgcagtcgac gaggatcatc ggcggtcggt gggtggaggc gatcgattca
     1861 gcctgggcga tgacttcggg ccggaacgat ccgacgagga tgacgccgtc gagcggctgg
     1921 cggagaagac gctccggata ctcggtcgct tcgttacggg aattgatcgg gatgtttgcc
     1981 aggaggagat tgattccctg gcctcccgca ctctcctgca agccggtaag cacccaagaa
     2041 tagaagtcga cgatgtttgg cgaggaattc gctcgctcgt aaccccaggt gcgaaagacg
     2101 atcgcaacat cgagcgtctg cctgtcggcg gtcggcatgg attgcacgcg ttgatagccg
     2161 agtgcccgtg ccgttgacag gatgcgctcg cgggtcgcgc ggctgactcc gggcttgttg
     2221 cgaagagcaa gcgagaccgt ggccggcgag caaccactcg ctgccgcgat atcgtcgatg
     2281 gtggcttgcc tcttcatcgc gtggtccctc tccggaaatt gagtggatgc gctggctcta
     2341 gcaatccttc atacgtatat caaatccaat tgaagcacgt tcatcgtctg gcaggcgtgg
     2401 atcgtgttgc gaaacgccga tcggcgaagc cgccggcacc ggttttccgg gccggcggtc
     2461 atcatgagat attcggggcg aaccgggccc gctactcggc ggcggccgcg aactcggcct
     2521 ttgcgcgcgc atagagttcc ggattcatga ggagatcggc ggcggcgagc gccagcgcct
     2581 tgcccgttgc gatcgcattc gcccgaccga gatcggagat cgaaccttcg accacctcga
     2641 tggagtggcc ggggatcggg accgtgctga tcgcgaagtt gaccgccgat gtcggcatct
     2701 tgcggctgac attgccaagg tcggtcgagt atggcgcggg accggtctgc cgtggcttct
     2761 gatcgacggg agtctccgcg agcccggccg gttcgagctt ctcctggagc aggcgaccga
     2821 gcgtgtagtt cggcaggaca tcgtcgtagt cgggctggct ggcatcgatc tcgaggcggg
     2881 cgccggtcat caatgctgct ccttccgcga tcttgaccgc cttttcgacg atctcgtcta
     2941 ccttgccgcg cgtcgcgccg cggaacatga aataggcttc ggcgtaatcc ggcaccacat
     3001 tcggcgccac gccgcccttc gtgatgatcc cgtggatgcg gacgtccggc gtcacatgct
     3061 ggcgcaaggc gtcgatccct gtgaagaggt ggatcaccgc attcagggca ttgatcccgt
     3121 tgtgggggtc gagcgcggcg tgcgcggtgc ggccgtggaa cttgaagatg cgcggcgaaa
     3181 ccgccaggca ggttccttcc ggctcatcgg tcgggacgcc gttgatattg ccggcgtgat
     3241 ggctcg
//
LOCUS       DASTMD010000790         4886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3109209, whole genome shotgun sequence.
ACCESSION   DASTMD010000790 DASTMD010000000
VERSION     DASTMD010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3109209"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VFQ54_11725"
     CDS             complement(<1..843)
                     /locus_tag="VFQ54_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165704.1"
                     /translation="MKAIGVLCARVRVEEKQIITAVGVAGHAGMPVPPPSTPMPPGPA
                     SLDLATLGGEEAANGAEGQSLDVVIDRCPDRLLASVTLPIMRTAGIRILAAGLAGEGT
                     RLDVARALAVAGVPRPNCLVAFSEESGVEAASRIGYPSTLLPLTPGSGTTAMLDQDTA
                     DAVIEHRVVLGKGPAAVILIQQGAPRDDERTIVHVVGGRAIAVEGNVTEVESLAIAEH
                     AARVVGASIVAVELVLLAGKIVVWDLHPVADFRKATLTGEQTMGEAIAELAIAGDDIV
                     ESVES"
     gene            complement(901..1068)
                     /gene="lysW"
                     /locus_tag="VFQ54_11730"
     CDS             complement(901..1068)
                     /gene="lysW"
                     /locus_tag="VFQ54_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HEU0165705.1"
                     /translation="MATVECPECGAEIELEGVEKGEIVPCPECGADLEVVGTDPLALA
                     LAPEEGEDWGE"
     gene            complement(1143..2168)
                     /gene="argF"
                     /locus_tag="VFQ54_11735"
     CDS             complement(1143..2168)
                     /gene="argF"
                     /locus_tag="VFQ54_11735"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU0165706.1"
                     /translation="MNSTDTANEAPVEPEIGFDPGLRGRSILSDTDLSPIEILEILDT
                     AETLKDLRKRGISWPWLAGKTLGMIFQHPSTRTRTAFQAGMEQLGGQAIFLGVQDLQL
                     KRGETIADTAAMFSRYVSAVAARIAEPKDLYEFAAGASVPVFNGLTSIDHPIEVLGDL
                     LTLRERFGTLKGLKFAYLGDGNNVCHSLLLACAAVGVSFALAAPEAFRLGPDVMAKAE
                     ELAAVSGAELMVTDDPFAAAQDADAVYTDVHESMGETVIPGKLMALAPFRVTTKIMAV
                     AKPEAVFMHCLPLHRGQEVEAAVADGPQSIIFYQAENRLHVHKAVLLQVLHDERWVEE
                     AEIASIA"
     gene            complement(2340..3431)
                     /gene="argF"
                     /locus_tag="VFQ54_11740"
     CDS             complement(2340..3431)
                     /gene="argF"
                     /locus_tag="VFQ54_11740"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU0165707.1"
                     /translation="MQNVSAPSAITAEPISAPVLHSVPSVQQLPGASAQERTVVLGGA
                     HALMQPAFTVSTRLRGRHVLVDTDLTPDELAEVIDTASRIKRAWRNGQPHAYLAGKTL
                     GLIFQHPSTRTRVSLEAGMAQLGGQAIFLGVNDLQMKRGETIGDTAQILSRYVDALVA
                     RVADHDDLITLATSASIPVMNGLSDKCHPLQAYADMLTLQEAFGSLKGLKLAYLGDGN
                     NMASSLMLSGAAMGVSVSVASPASLQPDPGVVAHAEWLGKNTGATIEVTTDPFAAAKD
                     ADAVYTDVHVSMGQEDAAGRAVALAPFKVTPEIMAAAKKTAVFMHCLPMHRDEEVSAV
                     VADGPQSIIFDQAENRLHMHKALLLQTLC"
     gene            complement(4152..>4886)
                     /locus_tag="VFQ54_11745"
     CDS             complement(4152..>4886)
                     /locus_tag="VFQ54_11745"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU0165708.1"
                     /translation="LQELQGYNPLQLNDYAAFINALNGRPQNYHFLDVWQSGVQSPLL
                     DMLNVRYIVVPRSLANRSDVQKLAEGRTIVYSDTLVNVYENPKAYDRAWIVHDVQQAT
                     DAEALSQLASGTADGHTVAFVNGQLPKVVAGDGPGGATESVTVTSYAPEKIVLDAHAA
                     NAGFMVLSEIYADGWRATVNGKSVKVYKTDGALRGVEIPAGESRIVMTYAPTSLRIGL
                     AVSGVAGIAMLATFATAGWYALRRHA"
BASE COUNT          827 a         1715 c         1476 g          868 t
ORIGIN      
        1 cgactccaca ctctccacga tgtcgtcccc ggcaatcgcg agctcggcga tcgcctcacc
       61 catcgtttgc tcgcccgtca acgtcgcctt ccggaaatcc gcgaccggat ggagatccca
      121 gacgacgatc ttgccggcga ggaggacgag ttcgaccgcg acgatcgagg cgccgaccac
      181 gcgcgcggca tgctcggcga tcgccagcga ctcgacctcg gtcacatttc cttcgaccgc
      241 gatcgcccgc ccgccaacca cgtgcacgat cgtgcgttcg tcgtcacgcg gggcgccctg
      301 ctggatgaga atcaccgcgg ctggcccctt gccgagcacg acccggtgct cgatcacggc
      361 gtccgccgtg tcctgatcga gcatcgccgt cgtccccgaa cccggagtca acggaagcag
      421 cgtcgatggg tagccgatcc ggctcgcggc ctcgactcca gactcttcgc tgaaggcgac
      481 gaggcagttc ggccgcggca cgccggccac ggcgagggca cgcgcgacat cgagccgtgt
      541 gccctcgccg gcgagaccgg cggccaggat ccggatgccg gccgttcgca tgatcggcag
      601 cgtcaccgac gccaggagcc gatccgggca gcgatcgatc acgacatcga gcgactgacc
      661 ctcggccccg ttcgcggctt cctcgccacc cagcgtcgcc agatcgagcg acgccgggcc
      721 gggaggcatg ggcgtgctgg ggggaggcac gggcatcccg gcgtgaccgg caacgccgac
      781 cgccgtgatg atctgcttct cttcgacccg cactcgtgcg cacagcacgc ctattgcctt
      841 caccgtgaac tctcctgtcg tcgttgccgc ttccggcagc cgaccatcac catggggcga
      901 ttactcgccc cagtcttcgc cttcttccgg cgccagcgcg agcgccagcg gatcagtccc
      961 gacgacctcg aggtcagcgc cgcactccgg gcaggggacg atctcgccct tctccacacc
     1021 ctcgagttcg atttccgcgc cgcactccgg gcattccacc gttgccatgt ttcctgttcc
     1081 tccagaaatt cgatcagatc gcccgccacg cgggttcatc cacacatacc cagctgattc
     1141 gctcaggcga tcgaggcgat ttccgcctct tcgacccaac gctcgtcatg cagcacctgc
     1201 aacaacaccg ccttgtggac gtgaagccga ttctcggcct gatagaagat gatcgactgg
     1261 ggaccatccg cgaccgccgc ctcgacttcc tgtccgcgat gcaatggcag gcagtgcatg
     1321 aagaccgctt ccggtttcgc caccgccatg atcttcgtcg tcacccggaa cggcgccagc
     1381 gccatcagct tcccgggaat caccgtctcg cccatgctct cgtggacgtc ggtgtacacc
     1441 gcatcggcat cctgtgccgc cgcgaacgga tcgtccgtca ccatcagctc cgcaccggac
     1501 accgcggcaa gttcctctgc cttggccatc acatccggac ccagccgaaa ggcttccggc
     1561 gccgccagcg cgaagctgac gccgaccgcc gcacaggcga gcaggaggga atgacagacg
     1621 ttgttaccat cgccgagata ggcgaacttg agccccttca gcgtgccaaa ccgctcgcgc
     1681 agcgtcagga gatcgcccag gacctcgatc ggatgatcga tcgatgtcag cccgttgaag
     1741 acgggcaccg acgcgccggc ggcgaactcg tagagatcct tcggctcggc gatccgtgcc
     1801 gcaaccgcgc tgacgtagcg actgaacatc gccgccgtat cggcgatcgt ctctccccgc
     1861 ttgagctgga gatcctgcac cccgaggaag atcgcctggc cgccaagctg ctccatgccc
     1921 gcctggaatg cggtccgcgt gcgcgtgctg ggatgctgga agatcatccc cagcgtcttt
     1981 cctgccagcc acggccagga gatgccccgc ttgcgaagat ccttcaaggt ctcggccgtg
     2041 tcgaggatct cgaggatctc gatcggcgag agatcggtat cggacaggat gctacgaccg
     2101 cgcaatcccg gatcgaagcc gatttccggc tcgaccggcg cctcgttcgc cgtgtctgtt
     2161 gaattcacgt cctcatcctg ttcgaccacc gaatggttct ccctggaaag ctgtccgcgt
     2221 tccgccggtc atcgactcgt gttctgtcca tgatggcgga acgaccgtta gtgccgtgtt
     2281 aaagaaaccc gataccgagc caagaatgcc gtgagaagga cggcaccccc acctcatcgc
     2341 tagcagagcg tctgcagcag cagcgccttg tgcatgtgga gccggttctc cgcctggtcg
     2401 aagatgatcg attgcggccc gtcggcaacc accgccgaca cttcctcgtc ccgatgcatc
     2461 ggcaggcagt gcatgaagac ggcggtcttc ttcgccgcgg ccatgatctc gggagtcacc
     2521 ttgaacggcg ccagcgcgac ggcgcgaccg gccgcatcct cctgccccat gctcacgtgg
     2581 acgtcggtgt agaccgcatc ggcatccttc gccgcggcga acggatcggt cgtgacctcg
     2641 atcgtcgcgc ccgtgttctt gccgagccat tccgcatggg cgacgacgcc gggatccggc
     2701 tggagggacg ccggggaggc gacggaaacg ctgaccccca tcgccgcgcc ggagagcatc
     2761 agcgacgacg ccatgttgtt gccatcaccg aggtaggcga gcttgagccc cttcagggag
     2821 ccaaaggcct cctgaagcgt cagcatgtcg gcatacgcct gcagcgggtg gcacttgtcc
     2881 gaaaggccgt tcatcacggg aatgctggcc gacgtcgcga gggtgatcag atcgtcgtga
     2941 tccgcaaccc gcgcgacgag cgcatcgaca tagcgactga ggatctgggc ggtatcgccg
     3001 atcgtctcgc cccgcttcat ctgcaggtcg ttgacgccga ggaagatcgc ctggccaccg
     3061 agctgtgcca tccccgcctc gagcgagacc cgggttcgtg tgcttgggtg ctggaagatg
     3121 aggccgaggg tcttgccggc aagataggca tgcggctgac cgtttcgcca ggcccgcttg
     3181 atccgcgatg ccgtgtcgat cacttcggcg agttcatccg gcgtgagatc ggtgtcgacc
     3241 agcacgtggc gaccgcgcag ccgggtgctg accgtgaagg ccggctgcat gagggcatgc
     3301 gcgccgccga gcacgaccgt gcgctcctgg gcggatgcac ccggaagctg ctggacactt
     3361 ggcacggaat gaagcacggg cgccgagatg ggttcggcgg taatggcgga cggggcggaa
     3421 acgttttgca cgaagttctc ccttcgatcg cggctcgcaa gttgatgcgg acgccggtca
     3481 ccgccggaat gttgccattg ccgaacggga ccgaaggggc ggggaacgtc tctacccgcg
     3541 gtaccaccct tcttgccgga gcgaatcgat tcgatcgctc cggcatctct cggctgttgt
     3601 gaccatggga gccacccatc cagacctact tgaccgaacc aggtcaccct ggttcgtctt
     3661 tcttcggccg gccgctctgg ggcgcgttcg gaggggctgc acattccccg ggcttgcaca
     3721 gtccccgggt cgctggtgtg cgccttcctc gtactcttcc ccgtcatcgc gggctgtcgt
     3781 gaggattcag tttgtgaaac gaacgaaagc tgtcttcgcg cttcggatcg cggacggcga
     3841 ccgtcagcgc gtcgtacgac gacgcgccga tggccgccgt cggggagagc gggaaagcaa
     3901 ctcctgctct acgcctggat gtgagtgttc gatcaacata tcgatcctcg cgcgatacaa
     3961 cgcgcgtcct cgagcgtagc tttcgtcagt cagtcgcgcc cgcggggctg gaaacagcca
     4021 acgggcgtcc tggagacacc ccaccccgca ttcgtgatat cggtcacgag cgcccagtga
     4081 ccttacccga gacggtatcc ccgtgtcaag tcgcgggacc gtttcttggg cgctgcgcat
     4141 ggatgcccga tctacgcgtg gcggcggagc gcgtaccagc cggcggtcgc gaaggtcgcc
     4201 agcatcgcga tccctgccac gccactcacc gccagcccga tccgcaacga cgtcggcgcg
     4261 tacgtcatca cgatccggct ctcccccgcc ggaatctcca ccccccgcaa cgccccatcc
     4321 gtcttgtaga ccttcacgct cttcccgttc accgtcgcgc gccacccatc cgcatagatc
     4381 tcgctcagca ccatgaagcc agcattcgcc gcatgcgcat cgagcacgat cttctccggc
     4441 gcgtatgacg tgaccgtcac gctctccgtc gctcctcccg gcccatcccc cgccacgacc
     4501 ttcggcagct gcccgttcac gaatgccacc gtgtgcccgt ccgccgtccc cgacgccagc
     4561 tgcgacagtg cctcggcatc cgtcgcctgt tgcacgtcat gcacgatcca cgcgcgatcg
     4621 tacgccttcg ggttctcgta gacattcacc agcgtatcgg agtagacgat cgtccgcccc
     4681 tcggcgagct tctgcacatc ggaccggttc gccaggctcc tcggcaccac gatgtaccgc
     4741 acgttcaaca tgtcgagcaa cggcgattga acgccactct gccagacgtc gaggaagtga
     4801 tagttctgcg ggcggccatt caaggcgttg atgaaggcgg cgtagtcgtt caactggagc
     4861 gggttgtacc cctgtagctc ctgcag
//
LOCUS       DASTMD010000791         4015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1771141, whole genome shotgun sequence.
ACCESSION   DASTMD010000791 DASTMD010000000
VERSION     DASTMD010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771141"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VFQ54_11750"
     CDS             complement(<1..1308)
                     /locus_tag="VFQ54_11750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0165709.1"
                     /translation="MPSAAPIATDTTRTGTASRRTPRVALQDWLWLLTAMLLSLALRI
                     PFFGIPMIPDEGGYAYATQGWMDGTGKLYHDLWISRPQGIFLVYGAIMETLGDGTYAF
                     RFAAWIAIALTTIAVWLFARMWINPTVANVAAILFAVLSSSPNLEGFTANAEIFMGLP
                     AAFAAVWLLQISRKSWSPWQLCGVGVLVGLATILKPSGVVMLPVAWGFILMIRQEQWR
                     VHLKRCSAVFAGVMLVAIPTIINGITLGWHQFLYATVTYRLFQQSSASVGLSHNVTRL
                     LSVSFQIWPMLALLLAVLAVAHRETIRRWIGRAPELVANGNRAMGWQMLRKIQSDVTI
                     GLITPAPVRLTNLQRPTDDAGIILRLWVFGCLGGIAMGGDWWSHYLIQIAAPAAIWLG
                     MISTRITIDLAMLRKRVAFVAMAVLLLGIPYRVLTDGSVTAMAE"
     gene            complement(1546..2295)
                     /locus_tag="VFQ54_11755"
     CDS             complement(1546..2295)
                     /locus_tag="VFQ54_11755"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU0165710.1"
                     /translation="MCTYTACMYVYPILREGKLPELDPTKNPGSSRRRAQQPASASPS
                     ATSNRTSPANQPVAVAGNPRSRSPRADAEATTSPRERGRFDSRMTALASLAIVVVIAI
                     GLFIWAFRLEGELHDTRHDLAAVTTERDTLRQNANATAYTLTPTSSAPANATGRAFLT
                     IQGSGVLTTANLPRLGTNQIYQVWFRASASTSPIPGGTFALDKNGNGFLLLSGDVGSF
                     QTLSVSVEPASGSTAPTTPMLLTVDVSGVRG"
     gene            2478..3743
                     /locus_tag="VFQ54_11760"
     CDS             2478..3743
                     /locus_tag="VFQ54_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165711.1"
                     /translation="MRRETRSAPPGVPETVGVVGRMARLGMAALLALTFLMAVSGAVN
                     QAAAQVNPATPASTATQTSNTPSSSQGNTANPATGPGAPAVLASGLVYLSGDDVVWSV
                     TELTLPDVADATPQTGDAAVLYQRSGASIARNDTTAKRAQVDTGTAYFVAAGDPYTIS
                     AIPGDKSLGWNFTIGASANVPTEAFYESPAISNISEGTYSESLTRYVLRADEEANVQP
                     VTGSSLVMVVSGQVEISDGTSTSQLATADGQLVAKNSTVHNTGSSPAVYVVLGLGTAV
                     SDDTAGSPLAPAATETPSGDTTAAPTEASSDTSSDTSGDTDAATATEAPSSGSDQPSG
                     GPYLAVINITAESDIYLTVTVDGTTIFDGNLLSGQSTGTMTGSDFEVYTTVGGSTLFT
                     DGCGNTFYMGYETGEANYTLTANADSCGP"
BASE COUNT          752 a         1248 c         1328 g          687 t
ORIGIN      
        1 ctccgccatc gccgtcacgc tgccgtcggt aagcacccgg taggggatgc cgaggagaag
       61 cacggccatc gcgacgaacg cgacgcgctt gcgcagcatt gccagatcga tcgtgatcct
      121 ggtcgagatc attccaagcc agatcgcggc gggcgcggcg atctggatca ggtaatggct
      181 ccaccagtcg ccacccatcg cgatgccacc gagacatccg aaaacccaga ggcggaggat
      241 gatgcccgcg tcgtcggttg gccgctgcag attggtgagg cggacgggtg cgggtgtgat
      301 caggccgatc gtgacgtccg actggatctt gcggagcatt tgccacccca tcgcacggtt
      361 gccattggca accagctccg gtgcgcgccc gatccagcgt cgaatcgtct cgcgatgggc
      421 gaccgcgagc acggcgagga gcaacgccag catcggccag atctggaagc tcaccgagag
      481 caggcgggtc acgttgtgac tgagcccgac cgatgcgctg ctctgctgga agaggcggta
      541 ggtaacggtc gcatagagga actgatgcca accgagcgtg atgccgttga tgatcgttgg
      601 aatcgcgacg agcatcacgc cggcgaacac ggcgctgcag cgcttcaggt ggacccgcca
      661 ctgctcctgc cggatcatca ggatgaatcc ccatgccacc gggagcatca cgacgcccga
      721 cggtttcagg atcgtcgcga gcccaaccag gacgccgacg ccgcagagct gccatggcga
      781 ccaggacttc cggctgatct gcaggagcca gaccgccgcg aacgcggccg gcagccccat
      841 gaagatctcg gcgttggcgg tgaatccctc gagattggga ctcgaactga ggactgcgaa
      901 gaggatcgcg gcgacattcg cgaccgtcgg gttgatccac atgcgcgcga agagccagac
      961 ggcgatcgtc gtcagcgcga tcgcgatcca ggccgcgaag cggaaggcgt aggtgccgtc
     1021 acccaacgtc tccatgattg cgccatagac gaggaagatg ccctgcggcc ggctgatcca
     1081 gagatcgtga tagagcttgc cggttccatc catccatccc tgcgtggcat aggcgtagcc
     1141 tccttcatcc ggaatcatcg ggattccgaa gaaggggatc cgcaacgcga ggctgaggag
     1201 catcgcggtc agcaaccaga gccaatcctg cagggctacc cgtggtgtcc tgcgggaggc
     1261 tgttccggtg cgtgtggtgt cggttgcgat gggggcggcg ctaggcatcg gcatcataca
     1321 tctttggagg agggtccctt tcccgaatgg agcacgttga caggtagaac gctcgggagc
     1381 cacgcgcgtg ggaatcgatc gcggcggtcg gtcgacaaaa aacaccgcat gaagagcgcc
     1441 agcttcaggc gacgcatcac atatgttccg ccaaggatac gacgttcggg gcttccgagt
     1501 tcccttttcg ccggattggg gccgaacgtt cgggcttccc gcggtctagc cgcgaacgcc
     1561 gctgacatcg acggtaagga gcatcggggt ggttggcgcc gtgcttccgc ttgcgggctc
     1621 caccgaaacc gaaagcgtct ggaagctccc cacgtctccc gagagcagca ggaatccatt
     1681 tccgttcttg tccaatgcaa aagtcccgcc aggaatcggc gaggtgctcg cactcgcccg
     1741 gaaccagacc tggtagatct ggttggtgcc gaggcgggga agattcgccg tcgtcagcac
     1801 gccggagccc tgaatggtca ggaaggcccg gccggtcgcg ttggcggggg cgctcgaggt
     1861 cggggtcagc gtgtaggcgg tggcattcgc gttctgccgg agtgtgtcgc gttccgtcgt
     1921 gactgccgcg agatcgtgcc gtgtgtcgtg caactctccc tcgagccgga acgcccagat
     1981 gaagagaccg atggcgatca cgacgacgat tgccagcgat gcgagtgccg tcatgcgcga
     2041 atcgaagcga cctctctccc gcggcgacgt cgttgcctcc gcgtctgcgc gcggcgaccg
     2101 cgaccggggg ttccccgcga ccgcgaccgg ctggttggcc ggggatgtgc ggttcgaagt
     2161 cgcggaaggg gacgcgctcg ccggttgttg cgctcgtcgc ctcgaggaac cgggattctt
     2221 tgttgggtcg agttctggca atttcccttc cctcaggata ggatagacat acatgcacgc
     2281 tgtgtacgta cacatagcga cggatgaatg cacccggcaa tggcagacgg ttgaccgctg
     2341 gtcggtgcgg atgcttgttc aggcgagttc gatgcgccag gcaccatggt acgagcacca
     2401 cacactcgct tgcgggtgat cggccgaatg ctccgtttcg atgagcaggc ctcgcaggcg
     2461 acgaagggag tatacgcatg agacgagaga cgcgatcggc accgccgggc gttccggaaa
     2521 ccgtgggggt ggtgggacga atggcccggc ttgggatggc ggcgctgctg gcgctgacct
     2581 ttctcatggc cgtctccggg gcggtgaacc aggccgccgc gcaggtgaat ccggcgaccc
     2641 cggcgtcaac cgcgacccag acgtcgaaca cgccgagttc atcgcagggc aacaccgcga
     2701 acccggcgac cgggccgggc gccccggcag tcctcgcatc cggacttgtc tacctgtcgg
     2761 gcgacgatgt ggtgtggtcg gtcaccgagc tgacgctgcc ggatgtcgcc gacgcgacac
     2821 cgcagaccgg tgacgcggcg gtcctctacc agcgctccgg cgcctcgatc gcccgcaatg
     2881 acaccaccgc gaagcgggcg caggtcgata cgggtaccgc ctacttcgtc gccgccggcg
     2941 atccttacac gatctctgcg atccccggtg acaagtcgct gggctggaac ttcaccatcg
     3001 gcgcctcggc caatgtcccg accgaggcct tctacgagag tccggcgatt tccaacatca
     3061 gcgagggaac ctactcggag tcgctgaccc ggtacgtgct ccgcgccgat gaagaagcga
     3121 atgtccagcc cgtgaccgga tcgtcactcg tcatggttgt cagcggccag gtcgagatca
     3181 gcgacggcac aagtaccagc caactcgcga cggctgacgg gcaactggtt gccaagaact
     3241 ccaccgtcca caacacggga tcgagcccgg cggtctacgt cgtgctcggg cttggtaccg
     3301 cagtttccga cgacaccgcg gggagccccc ttgccccggc agcgaccgaa acgccgtccg
     3361 gcgacacgac ggcggcgccg accgaggcaa gctcggacac gtcgagcgat acctccggtg
     3421 ataccgatgc cgcgacggca accgaggccc cctcgagtgg ctcggatcag ccatcgggcg
     3481 gcccctatct cgcggtgatc aacatcacgg cagagtccga tatctatctc accgtgacgg
     3541 tcgatggaac gacgatcttc gatggcaacc tgctcagcgg ccagagcacg ggtacgatga
     3601 cgggctccga tttcgaggtc tacaccaccg ttggcggcag cacgctcttt accgatgggt
     3661 gtggcaacac gttctacatg gggtatgaga cgggcgaggc caactacacg ctgacggcaa
     3721 acgcggattc gtgtggaccg tagcactttc gtctcgcgca tcgtatcctg cgagcacatg
     3781 aaacgcttgc tccagcagca atagggctca tgtgctcgca gtcgagtctg ggcggatcga
     3841 tcgcaaggaa ggggagagga acgtgaatcg tcgcaggatt ccacagtcag gcctgatttc
     3901 agagttcaag cagttccttt tgcgggggaa tgtcgttgat ctcgcgatcg cggtggtgat
     3961 cggcggtgcg tttaccgccg tcgtcaccgc actcgtgacc gatatcatca cgccg
//
LOCUS       DASTMD010000792         1833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2038431, whole genome shotgun sequence.
ACCESSION   DASTMD010000792 DASTMD010000000
VERSION     DASTMD010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2038431"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VFQ54_11765"
     CDS             complement(<1..640)
                     /locus_tag="VFQ54_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU0165712.1"
                     /translation="MAPRSNWKGYLKLSLVSCPVQLFPATSTKERVAFHLLNRETGHR
                     LRRKLVDPDTDEAVESEDQVKGFEVAKNEYILMEEDEIASVAIESTHTIDIETFVPKT
                     EIDATFLDTPYYLAPDGKMGEEAFAVIREAMREKGVVGIGRVVLYRRERPIMLEPRGK
                     GLVATTLRYAYEVRNDSDYFGDIDDVAVPEEMLDLASHIIERKAAHFDPKRFE"
     gene            991..>1833
                     /locus_tag="VFQ54_11770"
     CDS             991..>1833
                     /locus_tag="VFQ54_11770"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU0165713.1"
                     /translation="MTPTIHAEGEELWGLDPKLLRPCLALRDWLLEAGAKDDKQNILL
                     AGLAERLNALGVPIDRVTTAIDALHSEYSGVGRVWTRGVGSSIRLFPHGKEVEEIYKR
                     SPFAYVHETGEWLLLDLAETPDDRFGIVPELKEAGYVQYLCIPLVFTSGSKNGISFAS
                     YSRFTPQHLAILRFIMPSLAAAMEMRAVTQRLDNVLRIYVGDEPHRAILSGAIQRGQV
                     SRIRSAILFADMRSYTHITSMLEPEEAVALLNNFFDCLVPPIEAEGGEVLKYMGDGLL
                     AIFRD"
BASE COUNT          315 a          591 c          573 g          354 t
ORIGIN      
        1 cctcgaagcg tttcgggtcg aaatgggcag ccttccgttc gatgatgtga gaggcgagat
       61 cgagcatctc ctcggggacg gccacgtcgt cgatgtcgcc gaaatagtcg gaatcgttac
      121 ggacttcgta agcatagcgt aacgtggtgg cgacgagacc tttgcctcgc ggctcaagca
      181 tgatcggacg ttcgcggcga tagagcacca cccgcccgat cccgacgacg cccttttcgc
      241 gcatcgcctc gcggatgacc gcgaaggcct cctcgcccat cttgccgtcg ggtgcgagat
      301 aataaggggt gtcgaggaag gtcgcgtcga tctcggtctt gggtacgaac gtctcgatgt
      361 cgatcgtatg ggtcgactcg atcgcgaccg aggcgatctc gtcctcctcc atgaggatgt
      421 attcgttctt ggcgacttcg aagcccttga cctgatcctc gctctcgacc gcctcgtcgg
      481 tgtcggggtc gaccagcttg cgccgaaggc gatgccccgt ctcacggttc aggagatgaa
      541 acgccacgcg ctccttggtc gacgtcgccg gaaagagctg caccgggcac gaaaccagag
      601 atagcttaag atagcctttc cagttggaac gtggcgccat cgaagaactc cgtacgcatg
      661 cggccacacg gcttggtcac aaccgagtcg acgcggaaca acgactcctg gagatgagtc
      721 gttccgtccg gttcgactca tttccgattc gctccctcgg atcggacgat tcggctatgc
      781 tttgcgatgc gtccggttac cggagcgagc cgcccattct gttggcttct cctgccgtgt
      841 gcggtgcgaa gcgccttccg gggcggctgc atgcacccaa ggcgcgcccg tgccgtcccg
      901 atcggtttcc gcccgacgac ggttgcgcgc atggcgcgcg cgggccaatt acgtcggatg
      961 ttgaaccccg ccgcctccga gcctccgaac gtgaccccga caatccacgc ggagggggaa
     1021 gagctatggg ggctcgatcc gaaattgctg cgcccctgcc tcgcgctgcg cgattggctg
     1081 ctcgaggccg gcgcgaagga cgacaaacag aacatcctgc tggccggtct cgccgagcgc
     1141 ctgaatgcgc tcggggtccc gatcgatcgc gtcacgaccg cgatcgacgc gctccattcc
     1201 gaatattccg gcgtggggcg ggtgtggacg cggggcgtcg gttccagcat ccgccttttc
     1261 ccgcacggga aggaggtcga agagatctac aagcgcagcc cctttgccta tgtccacgag
     1321 acgggcgaat ggctgctcct ggacctcgcc gagacgcccg atgaccgctt cggcatcgtg
     1381 ccggagctca aggaggcggg ttacgtccag tatctctgca ttccgctcgt cttcacctcg
     1441 gggagcaaga acgggatcag cttcgcgagc tattcgcggt tcacgcccca gcatctcgcg
     1501 atcctccgct tcatcatgcc gagcctcgcg gccgcgatgg agatgcgggc cgtcacgcag
     1561 cggctcgaca acgtgctgcg gatttacgtc ggcgacgagc ctcaccgggc gatcctctcg
     1621 ggcgccatcc agcgtggaca ggtcagccgg atccgctctg cgatcctctt tgccgacatg
     1681 cgcagctaca cgcatatcac ctcgatgctg gaacccgagg aggcggtcgc gctcctcaac
     1741 aacttcttcg actgcctggt gccgccgatc gaggccgaag gtggcgaggt tctgaaatat
     1801 atgggcgacg gcctgctggc gatcttccgc gac
//
LOCUS       DASTMD010000793         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1414744, whole genome shotgun sequence.
ACCESSION   DASTMD010000793 DASTMD010000000
VERSION     DASTMD010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414744"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2604
                     /locus_tag="VFQ54_11775"
     CDS             <1..2604
                     /locus_tag="VFQ54_11775"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU0165714.1"
                     /translation="QTFNKFGLGILGEYFPAERAELITQPELRAIQREIGAFPILSWD
                     ERQRDAIELFRAMKDQGYRPLEADREKVTKWLRTRYLELPTDGDLFDFEGSGDSGGGD
                     ATAGVDRQLGEVFDGFLRYEQELRARGKMDYQDQKLRPLIRLVRDAKAREQARQTYDE
                     IIVDEAQDISRLDALLIWHVMGPQTTITLAGDDDQTIYEFKQASSVFLRDAARYFERD
                     TFETFLLTMNYRSPEAILAPAQRLIAHNVERIEKSVAAARSDLGEVIVQASATPEARV
                     KAVVAWIADNAGHNDSRFAWSDVGILAPSQKQLEVFDQALRAQKIPTKLNTGRNDDGT
                     IVNAVELSTIHKAKGRQWAVAFLPLSSEGEMPTVQSVRHGNLEAERRAYYVSMSRAAD
                     ILVVGYVRRDPHDTIARSTTGEIIGTSGASRFLFEAGLVTEWHDPAAERATVGGDSSA
                     STGTASVADRLRQRGKDGLQRIGRIAPRPEPTLDPDASDEEPASSTPAGGASVPASPE
                     EPEAHVEGEGTLPPSRPLAPQSLSDDLLHYVVKAERQAQMGEYRYAAADAWIVVESVI
                     AALSAEMTWSDKAIDRIDYLRTEKLLPQKMISRLHRFRIARNSYLHAETKRALSESER
                     VAATQEMIDHLRPFLSMVLRQPVTAEIPAKGPEPVTTVSPAPVPDPAGAVGKQARSSS
                     ATSSTPSNAIEQSGPTLYKIPTDRVMHVLQVLNAGGYDPERGRVIRQIGFHTVNAGVE
                     FLPFQLGMILFGVRYFIPQTFRYASSPIFARFCERHCAAMLPDQLRRTAKTPVAPELR
                     LFEVRIHELMQEILDRYLTLIPGQVDISAMLANRLESADVLQNGMNTSGVILEPLSSG
                     RYPRPSWTKRS"
     gene            2648..>3451
                     /locus_tag="VFQ54_11780"
     CDS             2648..>3451
                     /locus_tag="VFQ54_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165715.1"
                     /translation="MTDTLDSPAASPGPQAQAQPQPLDEISQAYVALAFGLDRHIPGA
                     IDGYMGPDALRPDPDAPAPEPAFLLAQAEALLAGIERADLPPARVDYLLAQATGMATM
                     CRKLVGEEIAYEDEVKLYFDVSPERTPESAYTEAIAALEDALPGDGTIGERMIAWRKQ
                     YEIPVEAAKGIVPVIVDEIRRRTFAFAALPDGEAVELSFVQDKPWSGYNWYLGSNRSL
                     IEINTDLPIKANALLDLMCHEGYPGHHTEHSLKEQLLYRDRGYGEHSILM"
BASE COUNT          658 a         1101 c         1119 g          573 t
ORIGIN      
        1 cagaccttca acaagttcgg gctcggtatt ctcggcgagt atttcccggc ggagcgggcc
       61 gagctcatca cccagccgga acttcgtgcg atccagcggg agatcggtgc gttccccatc
      121 ctttcatggg atgaacggca gcgcgacgcg atcgaactct tccgggcgat gaaggatcag
      181 ggataccggc cgctggaggc ggatcgggag aaggtaacga agtggcttcg caccagatat
      241 ctcgagctcc ccaccgatgg cgatctcttc gacttcgagg ggagtggcga ttccgggggc
      301 ggggatgcca ccgccggtgt cgatcgccag ctgggcgagg tcttcgacgg cttcctccgg
      361 tacgagcagg aactgcgcgc ccgggggaag atggattacc aggaccagaa gctgcgaccg
      421 ctgatccggc tcgtgcgtga cgcgaaggcg cgggagcagg cacggcagac gtacgacgag
      481 atcatcgtcg acgaggcgca ggacatcagc cggctcgacg ccctgctgat ctggcatgtg
      541 atgggaccgc agacgacgat caccctcgcc ggtgacgacg accagaccat ctacgaattc
      601 aagcaggcga gctcggtctt cctccgggat gcggcccggt acttcgagcg cgacacgttc
      661 gagaccttcc tgctcacgat gaactatcgc tcgcccgagg cgattctggc gcccgcccaa
      721 cgcctgatcg cgcataacgt cgagcggatc gagaagtcgg tcgccgccgc tcgcagtgac
      781 ctgggcgaag tcatcgttca ggcaagcgcg actccggaag cgcgggtgaa ggccgtcgtc
      841 gcctggatcg ccgataacgc tggacataac gacagtcgct ttgcctggag tgacgtcggg
      901 attctcgctc cgagccagaa acagctcgag gtcttcgacc aggcgttgcg ggcgcagaag
      961 ataccgacca agttgaatac tggccgcaac gatgacggga cgatcgtaaa cgcggtcgaa
     1021 ctcagtacga tccacaaggc aaagggccgc cagtgggcgg ttgcgtttct cccgttgagt
     1081 agtgaagggg agatgccgac cgtgcagagc gtccgccacg ggaatctcga ggcggagcgc
     1141 cgtgcctact acgtttcgat gagccgcgcc gcggacatcc tcgtcgtcgg atacgtccgg
     1201 cgcgatcccc acgacacgat cgcgcgatcc acgaccggcg agatcatcgg cacgagtggc
     1261 gcgagtcgct ttcttttcga ggcgggactc gtcacggaat ggcacgatcc cgccgccgag
     1321 cgagcgactg tcggtggcga ttcgtcggcg tcgaccggga ctgccagcgt cgccgatcgc
     1381 ctccgtcagc gcgggaagga tggtctccag cggatcgggc gtatcgcacc gcgacctgag
     1441 ccaaccctcg atccggacgc gtccgacgag gagccggcat catcaacgcc ggcaggtggc
     1501 gcttcggttc cagcatcacc tgaagagccg gaggctcacg tggaagggga ggggacgctg
     1561 ccgccgtcgc gtcctcttgc tccccagtcc ctgtcggatg atctgttgca ctatgtggtc
     1621 aaggccgagc gacaagcgca gatgggcgag taccggtacg cggccgctga tgcatggatc
     1681 gtggtcgaaa gcgtgatcgc ggcgctctcg gccgagatga cgtggagcga caaggcaatc
     1741 gacaggatcg attaccttcg gaccgagaag cttctgccgc aaaagatgat cagccggctg
     1801 cacagattcc ggatcgcacg caacagctac ctccacgcag agacgaagcg cgccctctcc
     1861 gaatcggaac gcgtcgcggc gacgcaggaa atgatcgatc acctgcggcc gtttctctcg
     1921 atggtgctcc gccaaccggt gaccgccgag ataccggcga aggggccgga gccggtgacc
     1981 acggtatcgc cagcgccggt accagatccg gcaggagccg tcgggaagca ggcccggtca
     2041 tcgagcgcga cgtcatccac gccatcgaat gcaatcgagc agtccggtcc cacgctctac
     2101 aagataccga ccgatcgcgt gatgcacgtg ctgcaggtgc tgaacgccgg cggctacgat
     2161 cccgaacggg gacgggtgat ccgccagatc ggcttccata ccgtcaatgc cggggtggaa
     2221 ttcctgccat tccagctcgg gatgatcctc ttcggtgttc gctacttcat tccgcagacg
     2281 tttcgctatg cgtcgagtcc gatcttcgcg cgcttctgcg agcggcattg cgcggcaatg
     2341 ctcccggatc agctgcgccg gacggcgaag acgccggtcg cacccgagct tcggctcttc
     2401 gaggtgcgga tccacgaact gatgcaggag atactcgatc gctatctgac actgatcccg
     2461 gggcaggtcg acatcagcgc gatgctcgcc aatcgtctcg aatccgccga tgtgttgcag
     2521 aatggcatga acaccagcgg tgtcatcctc gaaccgcttt cgtccggtcg ctatccgcga
     2581 ccaagctgga cgaagcgatc gtagatcggc ccgtgactca tcgtacgttg aaggaatgga
     2641 cgccgtcatg accgacaccc tcgattctcc cgccgcatct cccgggccgc aggcgcaggc
     2701 acaaccgcaa ccactcgacg agatctcgca ggcgtacgtc gcgctcgcgt tcgggctgga
     2761 ccggcatatc ccgggggcga tcgacggcta catgggaccg gatgcattgc gaccggatcc
     2821 cgacgcgccc gcgccggaac cggccttcct cctcgcccag gccgaggcgt tgctcgccgg
     2881 gatcgagcgg gccgacctgc cgccagcgcg cgtcgactat ctcctcgcgc aggcgaccgg
     2941 gatggccacg atgtgccgca agctcgtcgg cgaggagatc gcctacgagg acgaggtgaa
     3001 gctctacttc gacgtgagtc ccgagcgcac accggaatcc gcatacacgg aagcgatcgc
     3061 cgcgctcgag gatgcgctcc ccggtgacgg cacgatcggc gagcggatga tcgcctggcg
     3121 gaagcagtac gagattcccg tcgaggcggc gaagggaatc gtcccggtca tcgtcgacga
     3181 gattcgccgc cggaccttcg cgttcgcggc gcttccggat ggcgaggcgg tcgagctctc
     3241 cttcgtccag gacaagccgt ggagcggcta caactggtat ctggggtcga accgctcgct
     3301 gatcgagatc aacaccgatc tcccgatcaa ggcgaatgcg ctgctcgacc tgatgtgcca
     3361 cgaggggtat cccggacatc acaccgagca ctccctgaag gagcaactgc tctatcgcga
     3421 ccgcggctac ggcgagcatt cgatcctgat g
//
LOCUS       DASTMD010000794         4461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2484869, whole genome shotgun sequence.
ACCESSION   DASTMD010000794 DASTMD010000000
VERSION     DASTMD010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484869"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VFQ54_11785"
     CDS             <1..403
                     /locus_tag="VFQ54_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165716.1"
                     /translation="TLNYLVVCGSINYVLFEDGALGSTPLADFKKGVEQGLGDTGEGT
                     PDANGGVAKVTDVKVAKEDAVLLTYTLHDSSGVVAVVAELAIPVGNMFIISQVSVADT
                     TAPDEKDVDALANDLTVSAITYLGTAAKAAQ"
     gene            complement(474..998)
                     /locus_tag="VFQ54_11790"
     CDS             complement(474..998)
                     /locus_tag="VFQ54_11790"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEU0165717.1"
                     /translation="MIDDGIVQIDRGDITRERVDAIVNAANESLLGGGGVDGAIHRVA
                     GPALLDACRKLGGCPAGQARLTPGFRLPAKFVIHAVGPVWQGGGAGEDHLLASCYQAS
                     MAIANARGIRSISFPAISTGAYGFPMFRAARIAIRELHAAAQRFPRVQLIRIVCFNAE
                     TYRAYERAVEDQVD"
     gene            complement(1017..1898)
                     /gene="sucD"
                     /locus_tag="VFQ54_11795"
     CDS             complement(1017..1898)
                     /gene="sucD"
                     /locus_tag="VFQ54_11795"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU0165718.1"
                     /translation="MSILVGNNTRLIVQGITGREGSFHATQMVEYGTNVVAGVTPGKA
                     GAEVAGVPVYNSVADAVKETQANTSIIYIPARFAADAIYEAVDAGIELIVCISDFMAV
                     NEMVPAYAYVKQKGARLIGPNCPGLITPGEAKVGIMPGFIHKQGKVGLVSRSGTLTYE
                     VVDALTKAGLGQSTAVGIGGDPVIGTSYVDVLELFQNDPGTEAIVMIGEIGGNDEETA
                     AAFIKAHVTKPMAGFIAGRTAPEGKRMGHAGAIISGGGGTAAGKIEALEDAGVRVAES
                     PSQVPALLQQAIAALSA"
     gene            complement(1961..3103)
                     /gene="sucC"
                     /locus_tag="VFQ54_11800"
     CDS             complement(1961..3103)
                     /gene="sucC"
                     /locus_tag="VFQ54_11800"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEU0165719.1"
                     /translation="MNVHEYQAAEVLARYGVPVNPGLVATTPEEAVDAARELGGTVAV
                     KAQVHIGGRGKAGGIKIAHSPEDAGAAAAQILGMDIRGHIVHKVLVAKGAAIDKEYYL
                     GVVLDRPNRQILVMASAEGGIEIEEVAEHSPEKILRVNGDPMLGFHPYQARQLGFDLG
                     LPADKIAGFAQVVLALYKAYVQEDASLAEINPLILNKSGEWQALDSKMSFDDNAVPRH
                     PDIEALRDPEEEDPTELEARRAGLSFVKLDGEIGCIVNGAGLAMATMDAVKNAGGDPA
                     NFLDVSGGASADQVAKAFSLVTADPNVTAILINIFGGITRGDVVAQGVIEAMERVGVK
                     VPIVVRLSGTNAEEGKRMLAEAGLVGVGSMDEAAAAVVAASKSTVA"
     gene            3573..3983
                     /locus_tag="VFQ54_11805"
     CDS             3573..3983
                     /locus_tag="VFQ54_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEU0165720.1"
                     /translation="MSERTLTCRDCGQEFVFTTGEQEFYQQRGFSDPQRCPTCRQARK
                     AQRNTSGGGYSSGGGGGGYSSGGYGGGGGGSSYSSGGDRGGFSSAPRQLYPATCSECG
                     QQTEVPFNPTPGKPVYCRDCFQSRKSNTSAYDRW"
BASE COUNT          802 a         1491 c         1358 g          810 t
ORIGIN      
        1 gacgctgaac tatctcgtcg tctgcggcag catcaactac gtcctcttcg aggatggcgc
       61 actggggagc accccgctcg cggacttcaa gaagggcgtc gagcagggtc tcggtgatac
      121 gggtgaaggc acgccggacg ccaacggcgg agttgccaag gtgaccgacg tcaaggtcgc
      181 caaggaggac gccgttctcc tgacctacac cctgcacgac agcagcggcg tggtcgccgt
      241 cgttgccgaa ctcgcgattc cagtcggcaa catgttcatc atcagtcagg tttccgtggc
      301 ggacactacg gctcccgacg aaaaggatgt cgacgccctc gccaacgacc tgacggtctc
      361 cgcgatcacc tacctcggga ccgccgccaa ggccgcgcag tagacgcagc ctcgatcccg
      421 ataacggcag agaaccccgc gagcgttggc tcgcggggtt ctctgcgtcc gggtcagtcg
      481 acctgatcct cgaccgcccg ctcgtaggcg cgataggtct cggcattgaa gcaaacgatc
      541 cggatcagct ggacgcgggg aaatcgttga gcggcggcat gcaattcccg gatcgcgatc
      601 cgcgccgccc ggaacatcgg gaagccgtag gcgccggtgc tgatcgcggg gaaactgatc
      661 gaacgaatcc cgcgcgcatt cgcgatcgcc atgctcgcct gatagcagga ggcaaggagg
      721 tggtcctcgc cagcgcctcc accctgccag accggaccga cggcgtggat gacgaacttc
      781 gcgggcaggc ggaatcccgg cgtaagccgc gcctgccccg ccgggcagcc gccgagcttc
      841 cggcacgcgt cgagaagcgc cggtccggcc acgcgatgga tcgcgccgtc gacgccacca
      901 ccacccaaca gcgattcgtt cgccgcgttg acgatggcat cgactcgttc gcgcgtgatg
      961 tcgccgcgat cgatctggac aattccatcg tcaatcacgg cgcggcacac atcgtcctat
     1021 gcggagagag cggcgatcgc ctgctggagc agggccggca cctgtgacgg gctctcggcg
     1081 acgcggaccc cggcatcctc gagcgcctcg atcttgccgg cggcggttcc accgccaccg
     1141 gagatgatcg cgcccgcgtg tcccatgcgc ttgccttccg gtgccgtgcg accggcgatg
     1201 aagcccgcca tcggcttggt cacatgcgcc ttgatgaagg ccgccgccgt ctcctcgtca
     1261 ttgccgccga tctcgccgat catcacgatc gcctcggtgc cggggtcatt ctggaagagc
     1321 tcgagcacgt ccacgtacga tgtcccgatg acggggtcgc cgccgatccc gaccgccgtc
     1381 gactggccga gaccagcctt cgtcagcgcg tccacgacct cgtaggtcag ggtgccggag
     1441 cgcgacacca ggccgacctt gccctgcttg tgaatgaaac cgggcatgat cccgaccttc
     1501 gcctctcccg gcgtgatcag acccgggcag ttcggaccga tcagccgcgc gcccttctgc
     1561 ttcacgtagg cataggcggg caccatctcg ttgacggcca tgaaatccga gatgcagacg
     1621 atcagctcga tcccggcatc gaccgcctcg tagatcgcat cggcggcgaa gcgagccggg
     1681 atatagatga tggaggtgtt ggcctgcgtc tccttcaccg catcggcgac gctgttgtag
     1741 acgggaaccc cggcgacttc cgccccggcc ttgcccggcg tcacgccggc gacgacattc
     1801 gtgccgtact cgaccatctg cgtcgcgtgg aacgatccct cgcgtccggt gatgccctga
     1861 acgatgagac gcgtgttgtt accgaccaga atgctcacgg ttgaactccc tcagcggact
     1921 atcccgcggc gccacaactc cgttcacgcc gcgattcaga tcaggcaact gtgctcttcg
     1981 acgcggcgac cacggcagcg gcggcctcgt ccatcgatcc cacgccaacc aggccagctt
     2041 cggcaagcat gcgcttgcct tcctccgcgt tggtcccgga gaggcggacg acgatcggca
     2101 ctttcacgcc gacccgctcc atcgcctcga tcacaccctg cgcgacgaca tcgccgcggg
     2161 tgatcccacc gaagatgttg atcaggatcg ccgtgacgtt cggatcggcg gtgacgagcg
     2221 agaacgcctt cgccacctga tccgccgacg caccgccgct gacatccagg aagttcgcgg
     2281 gatcgccgcc cgcattcttg actgcgtcca tcgtcgccat cgcgagaccg gcaccgttga
     2341 cgatgcagcc gatctcacca tccagcttca caaaggaaag tccggcccgg cgcgcttcga
     2401 gctccgtcgg atcctcctct tccggatcgc gcagcgcctc gatgtccgga tggcggggca
     2461 cggcgttgtc gtcgaagctc atcttcgagt ccagcgcctg ccattcacct gacttgttga
     2521 ggatgagcgg attgatctcg gcaaggctcg cgtcctcctg gacgtacgcc ttgtagagcg
     2581 cgaggacgac ctgggcgaag cctgcgatct tgtcggccgg caggccgaga tcgaagccaa
     2641 gctgacgcgc ctgataggga tgaaagccga gcatcggatc gccgttgacg cggagaatct
     2701 tctccgggct atgctcggcg acttcctcga tctcgatccc gccctcggca ctggccatca
     2761 ccaggatctg gcgattcgga cgatcgagca cgacaccgag gtagtactcc ttgtcgatcg
     2821 cggcgccctt tgcgaccagg actttgtgga cgatatgacc gcgaatatcc atgccgagga
     2881 tctgggcggc ggcggccccg gcatcctccg gggaatgcgc gatcttgatc ccacccgcct
     2941 tgccgcgccc gccgatatgc acctgggcct tgaccgcgac cgtgccgccg agctcgcgag
     3001 cggcatcgac cgcttcctcc ggcgtcgtcg cgacgagtcc cgggttcact ggcacgccat
     3061 atcgcgcgag gacttctgcc gcctggtact catggacatt catgaccaga attgctcctt
     3121 ttgcccggcg cggcaacgga tcggcgacag ccctgtcgct catcaaatct ccgcggacgc
     3181 gccggcgacg tgcatccact acgggatgct accacggtct ctccggaatc ccattggtcc
     3241 cttcggcggg agttgatggt catgctcgtg cggtatgacc gatggaatgt tcaccgatga
     3301 agttgcttat cgaaagtgag ttcataagtg aaaccgaagt tgccaaacgc accgaaaagg
     3361 ctatccgatc cgtcgaatgg aattgcgaaa aaagagttga catccgggaa cgcgtggtat
     3421 aatgacccca cacgggacgt ttccataggt agcgctcgac tggacccagg cggtgttcgt
     3481 tcgtgtcatg tccggggacc gtttcggcgt aggcattttt ccattcaggt tttccgccat
     3541 gtttcgatca tggcaagaga ggttcaagcg gaatgagcga acgaacgctg acctgccgtg
     3601 attgcggtca ggaattcgtg ttcacgactg gtgagcagga attttatcag cagcggggat
     3661 tttcagatcc tcagcgttgc ccaacgtgcc gccaggcgcg caaggcgcag cgaaacacat
     3721 caggaggcgg atattcgagc ggtggaggcg gcgggggata ctcgtccggc ggatacggcg
     3781 gcgggggcgg tggcagcagc tactcgtccg gcggcgatcg cggcggcttt tcatccgccc
     3841 cacgacagct ctacccggcg acctgttcgg aatgcggtca gcagaccgag gttccgttca
     3901 accccacgcc cggcaagccg gtctactgcc gcgattgctt ccagtcccgg aagtccaaca
     3961 cgtcggctta cgatcgctgg tagacagcgc gattgattga tcgatctcgc gcagtgagtc
     4021 cagtgcacag acccgggaca tatgattccg tctgtgctgc atgttcacga tcgtcgggtt
     4081 cagtatgttc ccggatgacg agcgcaaaga ccgcctatcg atgacgcggc cgggtgaagc
     4141 agttcacccg gccgtttctc tgcgcccatt ccgccactcc acaggaaagt gcagtacgct
     4201 atcgtgatcg tgctgcggaa gcgcagtcat ccgccgagat tacggcacgc gtcgccgtgc
     4261 cccagccatg gacctcgcta tcatgacatc ccaatccgag tccgtctcac ggcccgcacc
     4321 gatcgtcgtt cgctccggtc ggctggtcga cctctgcgag catgaagcct ccaaccggga
     4381 tcgattcgtc tcctggtatg gcgacgccga gatcgcggaa atgctccgcc acgatctcag
     4441 ccccctctca cccgcccgcg c
//
LOCUS       DASTMD010000795         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_787718, whole genome shotgun sequence.
ACCESSION   DASTMD010000795 DASTMD010000000
VERSION     DASTMD010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787718"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..2397)
                     /locus_tag="VFQ54_11810"
     CDS             complement(100..2397)
                     /locus_tag="VFQ54_11810"
                     /inference="COORDINATES: protein motif:HMM:NF015089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU0165721.1"
                     /translation="MTESDQLQPQPHTIASNFADRNQFLPSDVDALLYGTDPTPGIVA
                     AEFRSPNQILLYQRMPDNSTVAVTREFRPWMIARDPDSLQNLRPAPAASSLRGGETHP
                     LATLLEFDSWDHYRRSYDILPARSNGIYQISAPVSQFLMRSGVTLFKEMSFEDLRRLQ
                     LDIETLSLDPKDPDGEIVMIAMKMGDREELLFLETDERDLLVRFVDTLNGFDPDVIEG
                     HNIYNFDLPYLIERARRWQVPLRVGRDGSAPLLNEQQQRFRVGPVSLPYTLVYIHGRH
                     IVDTYQQVQRFDTAGRLSSYALKNAIRALGLERQDREFVAGEEIAELWRNGERDRERL
                     GRYAMDDVRDVDLLARVTIPTEFYQTQILPMTFQRTTVTGTGRKIDDLMVRAYLAAGH
                     GLPVAGTPRPYPGGFTELLRSGIFGPIVKCDVESLYPSIMLHDNVASTADTLRAFPVL
                     LRELTKRRIDAKQRARQTTGEEHALWDGLQSSFKVLINSFYGYLGFGGGLFNDFDAAE
                     QVTLEGQRMIQRVVAELDAEGAQPIEVDTDGVYFTPPDGISSEDEEEAFIAFVGATAL
                     PDGVRLAHDGRYEKMLSLKLKTYALLDYDGTLTLKGSALRSRRMERCFLRFIHDAARD
                     LMRGDRDEVRDHYFALAESIQQKTVDPKEISQWTMVNRNTLEKQPRFKALLDANPGRW
                     RFGERVEIYEREDGSLGLLEDYARDENTRGLLRRLSDTAARFTEAFPTQSEFDAFFPK
                     VTPTTRLSEAREAKPVQQLSLFGGG"
BASE COUNT          458 a          744 c          788 g          481 t
ORIGIN      
        1 tgccttcccc cccgtgttct ttccacgtaa aaggccgaag cagatcgatc tgcctcggcc
       61 ttttgtcatg cccgtgagag tggtcatgac gccgcgggat catccgccgc caaagaggct
      121 cagctgctgg accggtttgg cttccctcgc ctcgctcagg cgtgtcgtgg gagtcacctt
      181 cgggaagaag gcgtcgaact ccgactgcgt cgggaatgct tccgtgaagc gagctgccgt
      241 gtcggagagg cgccgcagca gaccgcgtgt gttctcatcc cgcgcgtagt cctccagcaa
      301 gccgagggaa ccgtcttcac gctcatagat ctccacccgc tcaccgaagc gccagcgacc
      361 ggggttcgca tcgaggagtg ccttgaaccg aggctgcttc tccagcgtgt tgcgattcac
      421 catcgtccat tgactgatct ccttgggatc aaccgtcttc tgctgaatcg actccgccag
      481 ggcgaagtaa tggtcacgca cctcgtcccg gtcgccccgc atcaaatcgc gcgcggcgtc
      541 atggatgaag cgaaggaaac agcgttccat ccggcgactg cggagtgcac ttcccttcag
      601 cgtgagtgtg ccatcgtaat ccagcagggc atacgtcttg agcttgagtg agagcatctt
      661 ctcgtagcga ccgtcgtgcg cgagccggac cccatccggc aacgccgtgg cgccgacgaa
      721 tgcgatgaac gcctcttcct cgtcctcact ggaaatcccg tcgggaggcg tgaagtagac
      781 gccgtccgta tcgacttcga tcggttgtgc gccctcggcg tcgagttcgg cgacgacccg
      841 ctgaatcatg cgttggccct cgagcgtcac ttgttccgcc gcgtcgaaat cgttgaagag
      901 accgcccccg aaaccgagat acccgtagaa cgaattgatc agcaccttga agctgctctg
      961 caggccgtcc cagagggcgt gttcttcacc cgtcgtctga cgggcgcgct gcttggcgtc
     1021 gatacgtcgc ttcgtgagtt cgcgaaggag gacggggaac gcacggagcg tgtcggcggt
     1081 gcttgccacg ttgtcgtgca gcatgatcga cgggtaaagg ctctcgacat cgcacttcac
     1141 gatcggtcca aaaatcccgc tccggagcag ctcggtgaac ccgcccggat acggtcgcgg
     1201 agtgccggcg accgggagac cgtgaccagc tgcgagatac gccctgacca tgaggtcatc
     1261 gatcttgcga ccggtcccgg tgaccgtggt gcgctggaag gtcatcggca ggatctgcgt
     1321 ctgatagaac tccgtgggaa tcgttacccg cgcgaggaga tcgacgtccc ggacatcgtc
     1381 catcgcgtat cgtccgagtc gctcccgatc gcgttcgccg tttcgccaca gctcagcaat
     1441 ctcctcgccg gcaacgaact cacgatcctg acgctcgaga ccgagggcgc ggatcgcgtt
     1501 cttcagcgcg tagctggaaa gtcgtcccgc cgtatcgaac cgttgcacct gttggtaggt
     1561 gtcgacgatg tgccgcccgt gaatgtagac gagcgtgtag ggcaaggaga cggggccgac
     1621 ccggaatcgc tgttgctgtt cgttgagcag gggagccgag ccatctcgcc cgacgcgcaa
     1681 cggaacctgc catcgccggg cgcgttcgat caggtacggc aggtcgaagt tgtagatgtt
     1741 gtggccctcg atcacgtcag ggtcaaaccc gttcagcgtg tcgacgaagc ggaccaggag
     1801 atcgcgttca tccgtctcga ggaagagcag ctcttcccgg tcgcccatct tcatcgcgat
     1861 catcacgatc tcgccatccg ggtccttcgg atccaggctc agcgtctcga tgtcgagctg
     1921 gaggcgccga agatcctcga agctcatttc cttgaagagc gtcaccccgc tgcgcatgag
     1981 gaactgggaa acgggagcgg agatctggta gatgccattc gaccgcgcgg gcaggatgtc
     2041 gtaggagcgc cggtagtgat cccacgaatc gaactccagg agggtggcca ggggatgggt
     2101 ctccccgcca cggagtgacg aagccgccgg agccgggcgc agattctgaa gcgaatcggg
     2161 atcccgcgca atcatccacg ggcggaactc acgggtgacc gcgacggtcg agttgtcagg
     2221 catgcgctgg tagaggagaa tctggttcgg ggaccgaaac tctgcggcaa cgattcccgg
     2281 cgttggatcc gttccataga ggagcgcgtc gacgtcggag ggcaggaact gattgcgatc
     2341 ggcgaagttc gatgcgattg tgtgcggttg gggctgcagc tgatcggatt ccgtcatgtg
     2401 gatcctctct cgtcagggat gagcatcgca gggaatgatg acatgttgac gtgggtaaag
     2461 caggcgctgg t
//
LOCUS       DASTMD010000796         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_611808, whole genome shotgun sequence.
ACCESSION   DASTMD010000796 DASTMD010000000
VERSION     DASTMD010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611808"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VFQ54_11815"
     CDS             <1..893
                     /locus_tag="VFQ54_11815"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU0165722.1"
                     /translation="ATSGVVSKPVAVLNPAGDTAIMTVIPTTSPQDAKTESLIKDLRN
                     NVIPPVADPAGIHVYVGGATAIFADLSSTTSSRLPIFIALVAGLSVILLMAIFRSVLI
                     PLKAAVANLLSFFAGYGVLVLVFQKGFLGINDWAFGVDKLGPVESFLPIFLFAILFGL
                     SMDYEIFLISRVHEAWGHLHDNGKALRTGIGSSGRVVLSAGAIMTCVFIAFAFGPERT
                     IKEIGLGLGSAILIDVLIVRMIIVPSFMSLAGNANWWFPAWLDRILPNLNVEGEPTAL
                     PDEEDHADAPVATTTPVVAS"
     gene            complement(999..2153)
                     /locus_tag="VFQ54_11820"
     CDS             complement(999..2153)
                     /locus_tag="VFQ54_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0165723.1"
                     /translation="MKIERFDLERWMTTWELDVDFDIAESGILPLSIDEICSLIGETE
                     AAELERRIRETLLGYSEARGTLELRSIIADTYERATPDDILVTTGAIEANFLLFNTLL
                     EAGDHVVAVSPAYQQLHSVPRAIGCELDLWSVETEHGFAYDLDRLEALIRNNTKLIVI
                     NTPHNPTGAMLDESQLRDVVAIADRVGAWILSDEAYRWLEHPGGEKLPPPLHDLTDRA
                     VSVGTMSKPFGLPGLRIGWFAANADIAQRAWGLRDYTSLSPAKLSDIITQTVISQRDR
                     ILPRNGAIIAENMTTARAWFAGNADLASWTEPRAGLLAMMKYNLPIDSTTLADGLARD
                     VRVMLAPGASFGLEGHLRIGVGPRPDLFAEGLRRTAAYFAAIREKSAAAV"
BASE COUNT          395 a          744 c          756 g          463 t
ORIGIN      
        1 gcgccacatc tggtgtcgtg tcaaagccgg tcgccgtcct caacccggcg ggtgacacgg
       61 cgatcatgac cgtgatcccg accacctcgc cgcaggatgc gaagaccgag agcctgatca
      121 aggatcttcg caacaatgtg atcccacccg tcgccgatcc tgcggggatc cacgtctacg
      181 tcggtggcgc gaccgccatc ttcgctgacc tctcatcgac gacatcgagc cggctcccga
      241 tcttcattgc cctcgtcgcg ggcctgagcg tcattctcct gatggcgatc ttccgctcgg
      301 tcctgatccc gctcaaggcg gcggttgcga acctgctctc cttctttgcg ggctacggtg
      361 tcctggtgct ggtcttccag aaggggttcc tcggcatcaa cgactgggcc ttcggggtcg
      421 acaaactggg accggtcgaa tcctttctgc cgatcttcct ctttgcgatc ctcttcggcc
      481 tctcgatgga ctacgagatc ttcctgatca gccgcgtcca tgaggcatgg ggtcatctgc
      541 acgacaacgg gaaggcgctg cgcacgggaa tcggctcgtc ggggcgcgtg gtgctcagtg
      601 ccggcgcgat catgacctgc gtcttcattg ccttcgcctt cgggccggag cgaacgatca
      661 aggagatcgg actcggactc ggttccgcga tcctgatcga tgtcctcatt gtccgcatga
      721 tcatcgtgcc gagcttcatg tcactggcgg ggaacgccaa ctggtggttc ccggcgtggc
      781 tggaccggat cctgccgaac ctgaacgtcg agggcgagcc gacagctctc cctgatgagg
      841 aagatcacgc tgatgcgccg gtggcgacaa cgactccggt cgtggcctcc tagcccgcga
      901 atcgtccatg tgtttgcccg tgtatgcgga aggagccccg gtcaaccgac cggggctcct
      961 tcacgtttga ttgaaatcgc gacattcgaa ctggcgagct ataccgccgc ggcggacttc
     1021 tcgcgaatcg cggcgaagta ggcggcggtg cgacgaagtc cctcggcgaa gagatccggg
     1081 cgcggtccga ccccgatccg gagatgaccc tcgagtccga aggatgcacc cggcgcgagc
     1141 atcacgcgaa catcccgggc gagaccgtcg gcgagtgtcg tggagtcgat cgggaggttg
     1201 tacttcatca tcgcgagcag accggcgcgc ggttcggtcc acgatgcgag atcggcgttc
     1261 ccggcgaacc aggcgcgggc cgtcgtcatg ttctcggcga tgatcgcgcc gttgcgcggc
     1321 aggatcctgt cccgctgcga gatgaccgtc tgcgtgatga tgtcggagag tttggccgga
     1381 ctgagcgacg tgtagtcgcg gagtccccac gccctctgcg cgatatcggc gttggcggcg
     1441 aaccagccga tacggaggcc gggaagcccg aacggcttcg acatcgtgcc gacgctgacg
     1501 gcgcgatcgg tgaggtcgtg cagtggcggt ggcaacttct cgccgcccgg atgttccagc
     1561 cagcgatacg cctcgtcgga gaggatccac gcgccgacgc gatccgcgat cgcgacgaca
     1621 tcacgaagct ggctctcgtc gagcatcgcc ccggttggat tatgtggcgt gttgatcacg
     1681 atcagcttcg tgttgttgcg gatcagcgcc tcgagcctgt cgaggtcgta ggcgaatcca
     1741 tgctccgtct cgacgctcca caggtcgagc tcacagccga tcgcgcgggg aacgctgtgc
     1801 agctgctggt acgccgggga gacggcgacg acgtgatcgc cggcttcgag cagggtattg
     1861 aagaggagga agttcgcctc gatcgcgccg gtggtgacga gaatgtcatc cggcgtcgcc
     1921 cgctcgtagg tatcggcgat gatcgagcgg agctcgagcg tgccgcgcgc ttcggagtag
     1981 ccaagcagcg tctcccgaat cctgcgttcg agttcggctg cttccgtttc accgatcagc
     2041 gaacagatct cgtcgatcga gagcggaagg atgccgcttt cggcgatgtc gaaatcgacg
     2101 tcgagttccc aggtcgtcat ccatcgttcg aggtcaaaac gttcgatctt catcggtgct
     2161 cccatctgta agatcagaca tgcaacgtgc ctagcggtct gtcacgcatc aaatgccggc
     2221 gtgccgcatc cccaaaacca tcggtcgtag gggtcagcct cgtgctgacc cgcggccgcg
     2281 gaccccctct tgccggttga cgtgcttccc taacgatcat atcgggctgc gcccgatggg
     2341 ccacacgggg tggcccgc
//
LOCUS       DASTMD010000797         1707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2485035, whole genome shotgun sequence.
ACCESSION   DASTMD010000797 DASTMD010000000
VERSION     DASTMD010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2485035"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VFQ54_11825"
     CDS             <1..789
                     /locus_tag="VFQ54_11825"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEU0165724.1"
                     /translation="ALVTSTTTTMASGSPVGRGGLIGGTWALVSMATADGTVSAPTGL
                     VNYTLRLADDGSSLGLTADCNTGGGSYSLDDDTLTISHVITTLIACRDGSGGSQFARM
                     LDGVHTISWNGEHLILTSADGAKITLAPALSAATWQWLPDDGGQLATPAAADLSRYTI
                     TFGDDGSLSVTADCNKGNSSYLTNGDQIAIRGIALTRAYCPQGSLGGQFAARIETATA
                     YTIGNGQLTLTTPAGDLVFSAQATREGGPATPVNGPAPAATPAG"
     gene            844..>1707
                     /locus_tag="VFQ54_11830"
     CDS             844..>1707
                     /locus_tag="VFQ54_11830"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165725.1"
                     /translation="MTSLWRNHAFLRLWIASAISSLGSKVTATALPLTAAITLDASPG
                     QMAALVAAGQLPDLVFGLLAGSWVDRGRHRSFLVGSDLGRALLLGIIPASALFGFLSF
                     PLLIAVAFGSGALSVFFSVASVAILPAIVPKEQFVEANARLSLSDSVVALSGPGVAGA
                     LIQVMSAPKAILVDAVSYLMSGSLLKGLGGTTATSSHTTRSSLLSDIRSGLVELIATP
                     LLRALTISSAVFAAGLAIQGTVVMLFLTRTLSLAPATIGLVLACGGAGSLLGGVLAGR
                     ISRRFGAGPAII"
BASE COUNT          259 a          619 c          537 g          292 t
ORIGIN      
        1 gcattggtaa cgtctacgac cacgaccatg gcgtcgggtt ctcccgtcgg gcgcggcgga
       61 ctgatcgggg gaacctgggc gctcgtcagc atggcgaccg ccgacgggac cgtctccgcc
      121 ccaaccgggt tggtcaacta cacgctgcgc ctcgccgatg acggctcctc gctcgggctc
      181 accgcagatt gcaacaccgg cggcggctcc tattccctag acgacgacac gctgacgatt
      241 tcccacgtga tcacgaccct gatcgcctgc cgggacggat ccgggggaag ccagttcgca
      301 cggatgctcg atggtgtcca tacgatttcc tggaacggcg agcatctcat cctcaccagc
      361 gcggatggcg cgaagatcac actcgccccg gcgctgagcg cggcgacctg gcaatggctc
      421 ccggacgacg gaggccagct cgcaacgcca gccgcggccg atctctcccg ctacacgatc
      481 accttcggcg acgacggctc gctctccgtc accgccgact gcaacaaggg aaacagcagt
      541 tacctcacca acggcgatca gatcgccatt cgaggcatcg cgctgacgcg cgcctactgc
      601 ccgcaaggat cgctgggcgg gcagtttgcc gcccggatcg agacggcgac cgcctatacg
      661 atcggcaacg gccagctcac actgacgacg ccggcgggcg atctcgtctt ctccgcgcag
      721 gccacccggg agggtggacc agccactccg gtgaacgggc ccgcaccagc agcgacgccg
      781 gcaggctaac ggcgatcgcg gttacggccc gatcggacat cgtgcaggag acggacaagc
      841 gtcatgacga gtctctggcg gaaccacgcg tttctccggc tctggatcgc cagcgcgata
      901 tccagcctcg gcagcaaggt gacggcgacc gcgctcccgc tcaccgccgc gatcacgctg
      961 gacgcgtcgc ccgggcagat ggccgccctc gtcgccgcgg ggcaactgcc ggaccttgtc
     1021 ttcgggcttc ttgccggatc gtgggtcgac cgtgggcgcc accgatcctt cctcgtcgga
     1081 tcggatctcg gacgtgccct actgctgggg atcatcccgg ccagcgcgct cttcggcttc
     1141 ctcagcttcc cgcttctgat cgctgtcgcc ttcggcagcg gggcactctc cgtcttcttc
     1201 agcgttgcct cggtcgcgat cctcccggca atcgtcccga aggagcagtt cgtcgaagcg
     1261 aacgcccgcc tctcgctgag cgattcggtc gtcgccctga gcggaccagg tgtcgccggt
     1321 gcgctcatcc aggtgatgtc cgcaccgaaa gcgattctcg tcgacgccgt ctcctacctg
     1381 atgtcgggga gtctgctcaa ggggctcggc ggcacgacag cgacgtcatc gcacaccacg
     1441 cgttcatccc tgctctccga catccggagc ggtctcgtcg agttgatcgc gacgccgctg
     1501 ctgcgggcac tgacgatctc atccgcggtg ttcgcggccg ggctcgcaat ccagggcact
     1561 gtcgtcatgc tcttcctgac gcggacgctc tccctcgccc cggcgacgat cggtctggtc
     1621 ctcgcctgcg gcggagcagg ctcgctgctt gggggcgtcc tcgccgggcg gatctcgcga
     1681 cgcttcggcg cgggcccggc gatcatc
//
LOCUS       DASTMD010000798         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3109791, whole genome shotgun sequence.
ACCESSION   DASTMD010000798 DASTMD010000000
VERSION     DASTMD010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3109791"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFQ54_11835"
     CDS             <1..554
                     /locus_tag="VFQ54_11835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922182.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU0165726.1"
                     /translation="YIPTNVISITDGQIYLETDLFNGGIRPAINTGLSVSRVGGSAQI
                     RAMRSVAGSLRLDMASYRSLAAFAQFGTADLDPSTRRQIERGQRMTEVLKQPQYSPLP
                     VEEQVAILWAGSAGILDDVPVERIRQFESEYLEDLRTAHADLLGRIRDEKALPDDLVA
                     ALTDHAKAFKVSHSFSSSAAAAD"
     gene            574..1443
                     /gene="atpG"
                     /locus_tag="VFQ54_11840"
     CDS             574..1443
                     /gene="atpG"
                     /locus_tag="VFQ54_11840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEU0165727.1"
                     /translation="MPSPREIRRRVRSVKNLAQITRALEMVAASKMRRAQDRVLRSRP
                     YSDRLREVLGDLASAQAGGSMREFPLLAERTEIKKTALILVTPDRGLAGALTTNILRR
                     ATQYIRREAGAPVDVIAVGKKGVQFSSRTRQSLVAQFQGLGDAPVLDDIRPITKIAID
                     EFLAGNVDAVHLAFPKFVNTLVQRPEVVQLLPIVRPEKSGTTKDYIFEPDPVVVLNEI
                     LPRYVDVLVYQAVLDTVASFYSAQMVAMRNATDNAKELSSDLALALNKARQAQITNEV
                     AEISAGANAFRVE"
     gene            1533..>2898
                     /gene="atpD"
                     /locus_tag="VFQ54_11845"
     CDS             1533..>2898
                     /gene="atpD"
                     /locus_tag="VFQ54_11845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU0165728.1"
                     /translation="MVATATAPETKTGGATGKIIQVTGVVVDIEFPPDQLPEIYNAIE
                     TTMPDGSKLVLETQIHLGNDRVRAVAMSTTDGLRRGQDARDTGAAIAVPVGPGTLGRI
                     FNVTGDPIDELGPVKATEFRPIHAEAPSFAEQSTKVEILETGSKVIDLIAPFTRGGKV
                     GVFGGAGVGKTVVITELIRNIAAEFGGYSVFCGVGECTREGTALWGELEESGVRDQTA
                     LVFGQMNEPPGARLRIALTGLTMAEYFRDEGRDVLVFIDNIFRFVQAGSEVSALLGRM
                     PSAVGYQTTLANEMGQLQERITSTRTGSITSVQAVYVPADDYTDPAPSSVFAHLDATI
                     SLERSIAERGLFPAVDPLASTSRILDPLIVGEEHYNVARGVQQILQRYRDLQDIIAIL
                     GIEELSDDDKQIVERARRIELFMSQPYFTAEQFTGTPGRFVSLADTVRSFKEIIEGKH
                     DHLPQ"
BASE COUNT          505 a          975 c          917 g          501 t
ORIGIN      
        1 cctacatccc gaccaacgtg atctcgatca cggacggcca gatctacctc gagaccgacc
       61 tcttcaacgg cggtatccgc ccggcaatca acaccggtct ctcggtttcc cgcgtcggcg
      121 gttccgcgca gatccgggcg atgcgctcgg tggccggtag tctccgtctc gacatggcgt
      181 cctaccgctc gttggcggca ttcgcccagt tcggaacggc ggatctcgat ccatcgaccc
      241 ggcgccagat cgagcgcggg cagcggatga cggaagtcct gaagcagcca caatacagcc
      301 cgctcccggt cgaggagcag gtggcgattc tctgggcggg ttcggccggt atcctggatg
      361 acgttcccgt cgagcggatc cgtcagttcg agtccgagta tctcgaggac ctgcgcaccg
      421 cgcatgcgga tctgctcggt cgcattcgcg acgagaaggc gcttccggac gacctggtgg
      481 ccgcgctgac cgaccacgcg aaggcgttca aggtgtcgca tagctttagc tcgagcgctg
      541 cggcggccga ctagcacgag aggatgaggc ggcatgccaa gcccacgtga aatccgtcgc
      601 cgcgtacgca gcgtcaagaa cctggcgcag atcacgcgtg cgttggagat ggtcgctgca
      661 tcgaagatgc ggcgcgccca ggatcgcgtg ctgcgcagcc ggccatacag cgatcgcttg
      721 cgtgaggtcc tcggggacct cgcgtccgcc caggccggcg gctcgatgcg ggagttcccc
      781 cttcttgccg agcgcaccga gatcaagaag acggcgctca ttctcgtgac accggatcgc
      841 ggtctcgccg gagcgctgac cacgaacatc ctgcgccggg cgacgcagta catccgtcgt
      901 gaggccggcg cgccggtcga tgtgatcgcg gttggcaaga agggtgtcca gttctcttcc
      961 cgcacgcggc agagtctggt cgcccagttc cagggtctcg gcgatgcgcc ggtgctcgat
     1021 gacattcgcc cgatcacgaa gatcgcgatc gacgagtttc tcgcgggaaa cgtggatgcc
     1081 gtgcatctcg ccttcccgaa gttcgtgaat accctggtgc agcgaccgga agtcgtccag
     1141 ttgctgccga tcgttcggcc tgagaaatcg gggacgacga aggactacat cttcgagccg
     1201 gatccggtcg tcgtgttgaa cgagatcctg ccccggtacg tcgacgtgct cgtctatcag
     1261 gcggtcctcg acacggtcgc ctcgttctac tccgcccaga tggtcgcgat gcgcaacgcg
     1321 accgacaacg cgaaggagct ttcttcggat ctcgcgctcg cgctcaacaa ggcgcgtcag
     1381 gcacagatca cgaatgaagt cgcggaaatc tccgccggtg cgaatgcatt ccgggtcgaa
     1441 taggcgtttg gttcttcacc ctgaccgtcc gcagtcgccg gcatgcgttc gccggcgact
     1501 gaccaacatc tagtaccaag cgaggaccgc acatggtagc taccgcgacc gctccagaga
     1561 cgaagacggg cggcgccacc ggcaagatca ttcaggtcac cggcgtcgtc gtcgatatcg
     1621 aatttccgcc ggatcagctt ccggaaatct acaacgccat cgagaccacg atgccagatg
     1681 gctcgaagct ggtgctcgag acgcagatcc acctcggcaa cgatcgcgtt cgcgcggtcg
     1741 cgatgagcac gaccgacggt ctccgccgcg gccaggatgc ccgcgatacc ggagccgcga
     1801 tcgcggttcc ggtcggcccg ggcacgctcg gtcgcatctt caatgtgacg ggtgatccga
     1861 tcgacgagct cgggccggtc aaggccaccg agttccgccc gatccacgcc gaggcgccga
     1921 gcttcgccga acagtcgacg aaggtcgaaa tcctcgagac gggctccaag gtcatcgacc
     1981 tcatcgcgcc gttcacgcgc ggtggcaagg tcggcgtctt cggtggcgcc ggtgtcggca
     2041 agaccgtcgt catcaccgag ctgatccgca acatcgcggc cgagttcggt ggctactcgg
     2101 tcttctgcgg cgtcggcgag tgcacccgcg agggcacggc gctctggggc gagctcgagg
     2161 agtcgggcgt ccgcgaccag accgcgctgg tcttcggcca gatgaacgag ccgccgggag
     2221 ctcgccttcg tatcgccctc accggcctga cgatggcgga gtacttccgg gacgagggac
     2281 gcgacgtcct cgttttcatc gacaacatct tccgcttcgt gcaggcggga tccgaggtct
     2341 ccgcgcttct cggtcgtatg ccgagcgccg tggggtacca gacgacgctg gcgaacgaga
     2401 tggggcagct ccaggagcgc atcacctcga cccgcaccgg ctcgatcaca tccgtccagg
     2461 ccgtctacgt gccggccgac gactacaccg acccggcgcc gtcctccgtg ttcgcccacc
     2521 tcgacgcgac gatctcgctc gagcggtcga tcgccgagcg cggtctcttc ccggccgtcg
     2581 atccgctggc ctcgacctcc cgcatcctcg atccgctgat cgtgggcgag gagcactaca
     2641 acgtcgcgcg tggcgtccag cagatcctgc agcgctatcg cgacctgcag gacatcatcg
     2701 ccatcctcgg tatcgaggaa ctcagcgatg acgacaagca gatcgtcgag cgcgcgcgcc
     2761 gcatcgagct cttcatgtcg cagccgtact tcacggccga gcagttcacg ggcaccccgg
     2821 gacgattcgt ttcgctcgcc gatacggtgc gctccttcaa ggagatcatc gagggcaagc
     2881 acgaccacct cccgcaga
//
LOCUS       DASTMD010000799         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_879660, whole genome shotgun sequence.
ACCESSION   DASTMD010000799 DASTMD010000000
VERSION     DASTMD010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879660"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFQ54_11850"
     CDS             <1..378
                     /locus_tag="VFQ54_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165729.1"
                     /translation="PFPTLLDRLDKGDFSLSLTGFSFTPDGNQGTMYRSDAFYPAGFN
                     IARYANPEYDKLDDAQQRELDPAKRKDLLIQEANILAEDLPNAPIVFDQAIVASSPRV
                     HNFFPSGYFAAPVWQFSWTWLDS"
     gene            578..2410
                     /locus_tag="VFQ54_11855"
     CDS             578..2410
                     /locus_tag="VFQ54_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0165730.1"
                     /translation="MNRTPTSGDRISRRHLTRTAGAVAAIGLTPAIAGSAIAQSATPE
                     ASPGASPAASPAASPVAIQTPDPNSPFSTTSLPRDEALTKAIGTTRLSDPGSTGGDLI
                     EISISDAAILNPALRQDGNSYQITSKVYEPLMTADPVTGAFVPQLADSWEMSADGLRW
                     RFHIHPGVTFHDGTPLTADDVVFTYNATTDPDSLAPNRATIVAVLASVQKLDDLTVEL
                     VAKFPSSAFLFQTASLVPIMPKHIWEPIAVKDWGTAPGSTGQDPKQIIGTGPFTFVEW
                     VQNDHITLNKNPTYWLKDWTPVYDRYIFRVIADANAAVQSLLAGESDLAPLSPSQAQS
                     ITKSNPDFTVYNVGTPVFAFYMTNLDPSHTDLFKDVRVREALLYGIDRKLLVDTVYLG
                     YAEVADSYAQPPGSPGYDPAEMKTVYAYDPDKAKQLLSDAGWTAGDGGKLSKDGKAFS
                     FEIMYDESEPTDQQLIPYFQQAWGALGIDLKTKPMPFPSIIDAVNRRDYDMIQTSFGY
                     YAPDGNTGLLFRTDAQYPAGYNMAYYSNPEYDKLDDEQLHQLDAAKRKALLIQQSNVV
                     NDDLPWAVLVWLKAVSASHPRVHNLIPTGIAAWWTLLKVWVDKS"
BASE COUNT          489 a          904 c          692 g          418 t
ORIGIN      
        1 cccttcccga cgctgctcga tcgcctcgac aagggggact tctccctctc cctcaccggc
       61 ttctccttca ccccggacgg caatcaggga acgatgtatc gaagtgatgc cttctacccc
      121 gcgggcttca acatcgcgag gtacgcgaat ccggaatacg acaagctcga tgatgcccag
      181 cagcgggaac tcgatccggc gaagcgcaag gacctgctca tccaggaagc gaacatcctg
      241 gccgaggatc tgccgaacgc cccgatcgtc ttcgatcagg cgatcgtcgc ctcgagccca
      301 cgcgtccaca acttcttccc gagcggctac ttcgcggcgc cggtctggca gttttcctgg
      361 acctggctcg attcgtaggt cgccaacgcc gacaccttgc ggcgccccgg cgtcgattca
      421 tgcgcacagc acaagacccc gggtcagaca atctgacccg gggtctttca cagaatcggg
      481 gatgcacgta ggctgacgcc atcgcgttcg ctccgaaccg actcccaatc ccaatcccaa
      541 ttccagttct ccctgttcca ccacgatgag gagaccgatg aaccgcacac caacctccgg
      601 ggaccgcatc tctcgccgcc atctcaccag gaccgccggc gccgtggccg caatcggcct
      661 cactccggcg atcgccggct cggcgatcgc ccagagcgcc acgccggagg cttcgccagg
      721 agcctcacct gccgcctcgc ccgcggcatc accagtcgcg atccagacgc cggatcccaa
      781 ctcccccttc tcgaccacct ccctcccgcg agacgaggcg ttgacgaagg cgattggcac
      841 gaccagactc agcgatcccg gcagcactgg cggcgatctc atcgagatct cgatcagcga
      901 cgccgccatc ctcaatcccg cgctccggca ggatggcaac tcctaccaga tcacgtccaa
      961 ggtctacgag ccgctgatga ccgccgatcc ggtcaccggc gccttcgttc cacaactcgc
     1021 cgattcctgg gagatgagtg ccgacgggct gcgctggcgg tttcacattc atcccggcgt
     1081 cacctttcac gatgggacac cactgaccgc cgatgacgtc gtcttcacct acaacgcgac
     1141 gaccgatccc gacagcctcg cgccgaaccg ggcaacgatc gtcgccgtgc tcgccagcgt
     1201 ccagaagctg gacgacctca ccgtggaact cgtcgcgaag ttcccgagca gcgccttcct
     1261 cttccagacc gcgagtctcg tcccgatcat gccgaagcac atctgggagc cgatcgcggt
     1321 caaggattgg ggaacggcgc ccggttccac cggtcaggat ccgaagcaga tcatcggaac
     1381 gggccccttc accttcgtcg agtgggtcca gaacgaccac atcaccctga acaagaaccc
     1441 gacctactgg ctgaaggact ggacgccggt ctacgaccgc tacatcttcc gcgtgatcgc
     1501 ggacgccaac gcggccgtgc agtccctcct cgccggtgaa tccgatctcg cgccgctctc
     1561 gccatcgcag gcacagtcga tcacgaagag caatccggat ttcacggtct acaacgtggg
     1621 aacgccggtc tttgccttct acatgacgaa cctcgatccg tcgcacacgg atctcttcaa
     1681 ggacgtcagg gttcgcgagg cgcttctcta cgggatcgac cgcaagctgc tcgtcgatac
     1741 cgtctacctc ggctacgccg aggtcgcgga cagctatgcg cagccgccgg gatccccggg
     1801 ctacgatccg gccgagatga agacggtcta cgcctacgat ccggacaagg cgaagcagtt
     1861 gctgagcgat gccggctgga ccgccggtga tggcggcaag ctgagcaagg atggcaaggc
     1921 gttcagcttc gagatcatgt acgacgagtc ggagccgacc gaccagcagt tgatccccta
     1981 tttccagcag gcgtggggcg ccctcggcat cgatctgaag acgaagccga tgcccttccc
     2041 gagcatcatc gacgccgtca accgccgcga ctacgacatg atccagacct cgttcggcta
     2101 ctacgcgccg gacggcaaca ccgggctgct cttccgcacc gatgcccagt acccggccgg
     2161 ctacaacatg gcctattact ccaatccgga gtacgacaag ctcgatgacg agcagctcca
     2221 ccagctggat gccgcgaagc ggaaggcgct gctcatccag caatcgaatg tcgtcaacga
     2281 cgacctgccg tgggccgtcc tcgtctggct gaaggcggtc tccgcctccc acccgcgcgt
     2341 ccacaacctg atccccaccg gaatcgccgc ctggtggaca ctcctcaagg tctgggtcga
     2401 caagagctag agcaagggtc ggaagacgtg cataccgctc cacctgctgg tcgtcatcct
     2461 gagcttgtcg aaggatcccc gcgcgaagcg catccggcgc agc
//
LOCUS       DASTMD010000800         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1505200, whole genome shotgun sequence.
ACCESSION   DASTMD010000800 DASTMD010000000
VERSION     DASTMD010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505200"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFQ54_11860"
     CDS             <1..798
                     /locus_tag="VFQ54_11860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEU0165731.1"
                     /translation="FASPSTESVLGFEPSMLEGEHVSRLLSPADHHLVDHLVATVIAS
                     PRATATMEFQFVRNDGITRWFEVVGTNMLDVSEVSGIALQARDVSDRKRMEELLATQA
                     LLDPLTGLLNRRGIVDRIDRALARHGDGPFPHVAFIDLDGFKEVNDTFGHQVGDETLV
                     ATARRILDCLDGHGHAARLGGDEFVVLVEDVCAEDALALFARIQEYISTPMEIEGELW
                     ELRASIGIAGGAGGAVTTTSLLHDADVALYQMKGDRSGTPIRAGSSD"
     gene            819..2531
                     /locus_tag="VFQ54_11865"
     CDS             819..2531
                     /locus_tag="VFQ54_11865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEU0165732.1"
                     /translation="MKRTMPFAGERAFMSERDIGQRPEILAQARGWYTIRREEMSDEL
                     LASLWPTYTGGLSLAQFRIHLQEVAQGLIDNAVAPELMETELQHVGARVSDLCNFTLE
                     GLEELRQVVPMILTRDLPDHLSPFVESRIERILTNVIWGYCSRVSERGMFPAMESVFA
                     DGQDSGSNILDTYFTDHPLALGVQSIATEQVVAINQKFEDFFGYTLESFPAPDYGAIL
                     APESANDLTAVIEDLRVGRIESFYRHGALRHRLGHTVWYELHAFRMYDRQRVARWIGY
                     MFKPARSRAAVAGSDEFENARIRVLSELSLDPALIVASTRAIKYASAATERVLGFEPA
                     DVLDQPISMLVIENDHVLLEGLLTSLSKRPRATDRIELRLRRKDGGWRWFEIAGANLL
                     DVQEIAGYSLQARDITERKRLEQLLTMQAHLDPLTSLLNRRGMMERVDAALLARERVA
                     VLFIDLDYFKAINDRFGHIVGDIVLRTIAERLDSQLATLGIVGRLGGDEFLVLANSAD
                     RFHLQRIREEVVALLEEPIAIDGEVHLVGGSIGVAWSDPSVTTSTELLRKADVEMYRA
                     KSRR"
BASE COUNT          526 a          911 c          940 g          559 t
ORIGIN      
        1 ttcgcaagtc catccaccga atcggtgctg gggttcgaac cgagcatgct ggagggcgag
       61 catgtctcgc gtctgctctc tccggcggat catcacctcg tcgaccacct ggtggcgacc
      121 gtgatcgcct cgccccgggc gaccgcgacg atggagttcc agttcgttcg caacgacggg
      181 atcacgcggt ggttcgaagt cgtcgggacc aacatgctgg atgtctccga ggtgagtgga
      241 atcgcacttc aggcaaggga tgtctcggat cgcaagcgga tggaggagct tctcgcgact
      301 caggcgcttc tcgatcccct caccggcctc ctcaatcggc gaggaatcgt cgatcggatc
      361 gatcgtgcgt tggcacgaca tggggacggg ccctttccgc atgttgcctt catcgatctt
      421 gacgggttca aggaggtcaa cgacacgttc gggcaccagg tcggagatga aaccctcgtc
      481 gcgacggcgc gccggatcct ggattgcctc gacgggcatg gccacgcggc acgactcggg
      541 ggagacgagt tcgtggtcct ggtcgaggac gtctgtgccg aggatgcgtt ggcgctcttt
      601 gcacggatcc aggagtacat ctcgacaccg atggagatcg aaggcgagtt gtgggaactc
      661 cgcgccagca tcggtatcgc cggcggcgcg ggtggtgcgg tcaccacgac gagtctcctg
      721 catgacgcgg acgttgcgct ctaccagatg aaaggcgacc gctctggcac gccgattcgt
      781 gctggctcga gcgactgatg gagagtcagg atgaggcctt gaagcgaaca atgccgtttg
      841 ctggcgagcg ggcgttcatg agcgaacggg atatcgggca acgcccggag atcctcgctc
      901 aggctcgggg ctggtacacg attcggcggg aggaaatgtc ggacgaattg ctcgcttcgc
      961 tctggcccac atatacgggt gggttgagcc tggcgcaatt ccggattcac ctgcaggagg
     1021 tcgcgcaggg actgatcgac aatgccgtcg cgccggagtt gatggagacg gaactccagc
     1081 acgtgggagc gagggtttcc gatctctgca atttcacgct ggaggggctg gaagaacttc
     1141 gacaggtggt tccgatgatc ctgacgcgcg acctcccgga tcatctctcg cccttcgtcg
     1201 agtcgcgtat cgagcgcatc ctcacgaacg tgatctgggg gtattgttcc cgcgtcagcg
     1261 agcgcgggat gtttccggca atggaatccg tgttcgccga cggacaggac tccggatcga
     1321 atatcctcga cacctacttc accgatcatc ccctggccct cggtgtccag tcgatcgcga
     1381 cggagcaggt cgttgcgatc aaccagaagt tcgaggattt cttcggatac acgctcgagt
     1441 cgtttcccgc gccggactat ggtgcgatcc tcgcgccgga atcggccaac gacctgacgg
     1501 cagtgatcga ggatctccgg gtcggacgga tcgaatcctt ctaccggcac ggggcgttgc
     1561 ggcatcggct cggtcacacg gtctggtacg agcttcacgc gtttcgcatg tacgaccggc
     1621 aacgcgtcgc ccgctggatc ggctacatgt tcaaacctgc acgatctcgt gccgccgttg
     1681 ccggttccga cgagttcgag aatgcccgga tccgcgtgct ctccgagctt tccctggatc
     1741 ccgcgctcat cgtcgcctcg acccgggcga tcaagtacgc gagtgccgcg accgaacgcg
     1801 ttctcggttt cgaaccagcc gatgtcctcg accagccgat ctcgatgctc gtcatcgaga
     1861 acgaccatgt cctcctggag gggctgctca cctcgttgag caagaggccg cgcgcgacag
     1921 accggatcga actccggttg cggcggaagg acggtgggtg gcgctggttc gagatcgccg
     1981 gagcgaatct cctcgatgtt caggagatcg ccggctactc gctccaggcg cgcgacatca
     2041 ccgagcgaaa gcgcctcgag caactgctga cgatgcaggc ccatctcgat ccactgacga
     2101 gcctgctcaa tcggcgcggc atgatggaac gggtggacgc ggccctgctc gcccgtgaac
     2161 gggtcgccgt gctcttcatc gatctcgact acttcaaggc gatcaacgat cgcttcggcc
     2221 acatcgtggg cgatatcgtg ctccggacga tcgccgagcg actggacagc cagctggcaa
     2281 ccctcggtat cgtaggcagg ctgggtggcg atgaattcct cgtcctcgcg aacagcgcgg
     2341 atcgctttca tctgcagagg atccgggaag aagtcgtcgc actcctcgag gaaccgatcg
     2401 caatcgacgg tgaggtgcac ctcgtcggcg ggagcattgg tgtcgcctgg agcgatccct
     2461 cggtgacgac gagcaccgaa ttgctgcgca aggcggacgt cgaaatgtac cgggcaaaat
     2521 cccggcgtta ggcccgcgcg gcaccgtcgc cgcgcaggcc ttcgctgggc ggtccggagg
     2581 cggcggtccc gccccacgta cgaccggatg cctttccgaa cactgcgtcc gacccggcag
     2641 caggcggcaa ccgtttcggt tttcgccgtg gtcttccgcg cgccgttgcc gcgcatgccg
     2701 gcccgcgcgt gggaattggc tatacttgac gaaagcactg ccggggccga gcgcagcaga
     2761 tgcgcggttc cggcattctt catgtcagcg ggttgacggc tccagcctgt ccactcccct
     2821 acgatcagaa tcgagaacca atcgtcgccc atgagtgagc aattgcccgc caattccact
     2881 gcccatatcc ggaacttcag catcatcgcg cacatcgacc acggcaagtc gacgct
//
LOCUS       DASTMD010000801         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2575898, whole genome shotgun sequence.
ACCESSION   DASTMD010000801 DASTMD010000000
VERSION     DASTMD010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2575898"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFQ54_11870"
     CDS             <1..427
                     /locus_tag="VFQ54_11870"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEU0165733.1"
                     /translation="VLEPSSGIVEVRVAIEDITTQTAPYSSIPVTPINLGDGLTATID
                     PATVTVSIDASRSVLQTMSADDVKVRVDLKGLGPGTYNLTPDVTLPQRASWLGNDPAT
                     VKVVITRGTPGASPPASPQATPETSTPESERPKDATPSN"
     gene            complement(488..1153)
                     /locus_tag="VFQ54_11875"
     CDS             complement(488..1153)
                     /locus_tag="VFQ54_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0165734.1"
                     /translation="MNRGPSDAVTRWRIIIADDESLIRLDLREMLTHLGYEVIAEAGD
                     GRSALDLARRLRPDLLIMDIKMPDVDGISAAEELTRERIAPVVLLTAYSDQGLVERAR
                     EAGVVGYVVKPFREAELMPVIELSRARFDEFRMLEKEVGSLKDALETRKLIERAKGVL
                     MEVHGLREAEAFHRIRKTSMDARKSMKEVAEAILLAHEMEFSISSGNGASSSRAASEP
                     AES"
     gene            complement(1268..1344)
                     /locus_tag="VFQ54_11880"
     tRNA            complement(1268..1344)
                     /locus_tag="VFQ54_11880"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1308..1310),aa:Pro,seq:ggg)
BASE COUNT          282 a          515 c          476 g          270 t
ORIGIN      
        1 cgtcctagag ccctcctcgg gcatcgtgga ggtccgtgtc gcgatcgagg acatcacgac
       61 gcagacggcg ccctacagct cgatcccggt gacgccaatc aatcttggcg atggcctgac
      121 ggcgacgatc gaccctgcca cggtgacggt ctcgatcgac gcctcacggt cggtcctgca
      181 gacgatgtcg gcggacgacg tcaaggttcg ggtcgatctc aagggcctgg gaccgggcac
      241 ctacaacctg actcccgatg tgacgctgcc gcagcgcgcc tcgtggctcg gaaacgatcc
      301 agcgacggtc aaggtcgtga tcacccgcgg aacgcccgga gcgtcgcctc ccgcctcgcc
      361 acaagcgacc cctgaaacca gcacgcccga atccgaacgt ccaaaggacg cgaccccctc
      421 gaactgacgt acaaacgcaa agcgccccga ccggggtttc cggtcggggc gctttcactc
      481 gctatcttca ggattccgcg ggctcgctgg cggcgcggct cgaactcgcg ccgttgccgg
      541 acgagatcga gaactccatc tcgtgcgcga ggaggatcgc ctcggcgact tccttcatcg
      601 acttgcgggc gtccatgctg gtcttgcgga tccgatggaa cgcctcggcc tcgcgaagtc
      661 cgtggacctc catgaggacg cccttcgctc gttcgatcag cttgcgcgtc tcgagcgcgt
      721 ccttgaggct gccgacttcc ttctcgagca tccggaactc gtcgaagcga gcgcgggaga
      781 gttcgatcac gggcatcagc tcggcttcgc ggaatggctt gacgacgtag ccgacgacac
      841 ccgcttcgcg ggcgcgctcg acgagaccct gatcggaata ggccgtgagg agcacgaccg
      901 gcgcgatccg ctcccgggtg agttcctcgg ccgccgagat gccatcgaca tccggcatct
      961 tgatgtccat gatcaggaga tccggtcgca ggcggcgcgc gagatcgagc gccgaacgac
     1021 cgtcaccggc ctcggcgatg acttcataac cgagatgggt cagcatctcg cgaagatcga
     1081 gacgaatcag cgattcgtca tcggcaataa tgatccgcca tcgtgtcacg gcgtcggaag
     1141 gtccacgatt catatcacgc catccctcaa gaataggttc gccagatcgc cgttggtctt
     1201 cctggtaatc tgctgagagg aatgcggcga ggatagataa agggacggca gtgccgtccc
     1261 agaggattgg tcggggtgag aggattcgaa cctccgacca ccggtacccc ataccggtgc
     1321 gctaccgggc tgcgccacac cccgtcggtg actcccgcag gtgggattat acggcactcg
     1381 gaagagcaac cgcaattcac ccagcctgcg ggaccgccgc ccgcgcacga tcttccgaat
     1441 cggtcacgcg cgcgaacgtc gagtgggcca ggtcgactac ttgacctcgg ccttggcgcc
     1501 agcttcttcg agcttggcct tggcggcctc tgcctcgtcc ttg
//
LOCUS       DASTMD010000802         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_612066, whole genome shotgun sequence.
ACCESSION   DASTMD010000802 DASTMD010000000
VERSION     DASTMD010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612066"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFQ54_11885"
     CDS             complement(<1..479)
                     /locus_tag="VFQ54_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165735.1"
                     /translation="MTDAPHARGQRADWSSVPGEIVATIQTELGGEVVHVMSMTGGFS
                     PGIAARIETATGRRAFVKAVSAARNPVAPTFHRREILVASHMPNDARLPVPPLLWSHD
                     EGGDGWVVLVFADIDGHQPAQPWVESELDRVIAALDDLATALTPSPIAAGTVGSASE"
     gene            complement(505..1272)
                     /gene="dapB"
                     /locus_tag="VFQ54_11890"
     CDS             complement(505..1272)
                     /gene="dapB"
                     /locus_tag="VFQ54_11890"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU0165736.1"
                     /translation="MSSPSLHIGIIGIGGRMGREIVNAASLDPTVEVVGGTVRPGSGV
                     TVDGIPTYDDLTRLVPEVDVLIDFTTPETTIATVEQAVVSGIPLVIGTTGLSEAQLAV
                     VRDAATTIPVFYARSMAHGINALLRALPAIAQALTGYDIEIVEMHHRHKKDAPSGTAL
                     ALIEAVASGLASDPALPTNHTYGRSGYAPRQSGEIGFHSLRGGGNTGEHTIVFASDGE
                     EVRISHRALNRGAFAAGAVRAAREIAGKAPGWYGPPL"
     gene            complement(1272..2264)
                     /gene="dapA"
                     /locus_tag="VFQ54_11895"
     CDS             complement(1272..2264)
                     /gene="dapA"
                     /locus_tag="VFQ54_11895"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU0165737.1"
                     /translation="MTTTATSTTNSIDLRGTWTALITPFTTGGETVDETALRNLVEFQ
                     IANGVSGLVACGSTGETPTLSLEEFTRTVSIVVEQVAGRVPVMAGTGSNDTATTVART
                     RLASELGADIALVVMPWYNRPTQEGLYQHIRHVAEQSPLPIVIYNVPSRTGCDILPVT
                     VARLAQIENIIGIKEASGDVDRTSEIVHATDPSFVVLSGDDSLTLPIISVGGQGVISV
                     VSNIVPQAMSALTTAALAGDFRTARGIHMELVDLSRTMFVETNPVPVKAAAELLGICT
                     SEVRLPLVALAPASHALVLQTLLSCRFTADRIILGEEAARFHTALDEIAVGVAA"
BASE COUNT          432 a          802 c          920 g          419 t
ORIGIN      
        1 cattcgcttg cggaaccaac cgttccggca gcaatcgggg acggcgtgag ggcagtcgcc
       61 agatcgtcga gcgccgcgat cacccgatcg agctctgact ccacccacgg ttgcgccggc
      121 tgatgcccat cgatatccgc gaagacgagg acgacccagc catcgccgcc ctcatcgtgt
      181 gaccagagga gcggcggtac cggcaggcgg gcgtcgttcg gcatgtggga tgcgacgagg
      241 atctcgcgcc ggtgaaatgt tggtgcgacg ggattgcgcg cggcggaaac cgccttcacg
      301 aaggcgcgcc ggccggtcgc cgtttcgatg cgcgcggcga tgcccggcga gaagccgcca
      361 gtcattgaca tgacatggac gacctcgcca ccgagttcgg tctggatcgt ggcgacgatc
      421 tcgcccggca cgctcgacca atcggcgcgc tgcccgcgag cgtgtggggc gtcggtcatc
      481 atgctcttcc ttgataagga ttggctagag tggcgggccg taccaaccgg gagccttgcc
      541 ggcgatctcg cgagcggcgc gcacggctcc ggcggcgaac gcgccccggt tcaacgcgcg
      601 atgactgatc cggacttcct cgccatcgct ggcgaagacg atcgtgtgct cgcccgtgtt
      661 gccaccaccc cggagcgagt ggaagccgat ctcgccagac tgcctcggcg cgtagccgga
      721 ccttccatag gtatggttcg tcggcagcgc cggatcggac gccagtccgg acgcgaccgc
      781 ctcgatcagc gcgagcgcgg tgccggacgg cgcgtccttc ttgtgccggt ggtgcatctc
      841 gacgatctcg atatcgtagc cggtcagcgc ctgggcgatc gcaggaagcg cgcgcaggag
      901 ggcgttgatc ccgtgcgcca tgcttcgcgc atagaagacg ggaatcgtcg tcgcggcgtc
      961 gcggacgacc gccagctggg cctcgctgag tccggtggtg ccgatcacga gcgggatccc
     1021 cgagacaaca gcctgctcga ccgtcgcgat cgtcgtctcc ggcgtcgtga aatcgatcag
     1081 gacatcgacc tccggaacca gccgcgtgag atcgtcgtac gtcgggattc cgtcgaccgt
     1141 gacgccggat cccggacgca ccgtgccgcc cacgacctcg accgtcgggt cgagcgaggc
     1201 ggcgttgacg atctcgcggc ccatgcgacc gccgattccg atgatcccga tatgcaacga
     1261 gggtgatgac atcaggcggc gaccccgacc gcgatctcat cgagcgcggt gtggaagcgg
     1321 gcggcctcct cgccgaggat gatgcggtcg gcggtgaagc ggcagctgag cagcgtctgc
     1381 aggaccagcg cgtgcgaggc cggggcgaga gcgacgagcg gcaggcgaac ctccgacgtg
     1441 cagatgccga gcagctccgc cgctgccttc accggcaccg gattcgtctc gacgaacatc
     1501 gtgcgggaga gatcgacgag ttccatgtgg atgccacggg cggtgcggaa atccccggcg
     1561 agggcagccg tcgtcagcgc gctcatcgcc tgcgggacga tgttgctgac gaccgagatc
     1621 acgccctggc caccgacgct gatgatcggc agcgtcagcg aatcatcgcc ggagaggacg
     1681 acgaacgacg gatctgtcgc atggacgatc tcgctcgtgc gatcgacatc cccgctcgct
     1741 tccttgatcc cgatgatgtt ctcgatctgg gcgaggcggg cgacggtaac cggcaggatg
     1801 tcgcaaccgg tgcggctggg aacgttgtag atgacgatcg ggagcggcga ctgctcggcg
     1861 acgtggcgga tgtgctggta gagcccttcc tgggtcgggc ggttgtacca gggcatgacg
     1921 acgagggcga tatcggcgcc gagttcgctc gcgagccggg tgcgggcgac cgtcgtcgcg
     1981 gtgtcgttgg agccggtgcc ggccatcacc gggacgcgtc cggcgacctg ctcgacgacg
     2041 atcgagacgg tgcgggtgaa ctcctccagg gagagcgtcg gcgtctcacc ggtggaaccg
     2101 caggcgacga gcccgctgac gccgttggcg atctggaact cgacgaggtt gcgaagcgcg
     2161 gtttcgtcga ccgtctcacc gcccgtcgtg aagggggtga tgagggcggt ccaggtgccg
     2221 cggagatcga tgctgttcgt ggtgctggtg gcggtcgtgg tcatggaaat tcctcacaat
     2281 ggctgagcat gggtgtgacg ggacgaccgg accggcatca tgccgatccg agagcggtga
     2341 ccctggatgc ctgagacgaa cgatccgcgt ccgggcggaa cgtgagggcg gcgcgaagaa
     2401 gcaccgcctg tctcaggcgc agcgcttgga ggagtctttt cgagattgcg aggtggtgct
     2461 gaggagtcgg tttctgcaga gtctggtcac gttatcggcc tccggcggca aacacacgac
     2521 gtgcttcgga gaagcacctt cgcgcaagat cggggacagc cacatatgcc caa
//
LOCUS       DASTMD010000803         1893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_612147, whole genome shotgun sequence.
ACCESSION   DASTMD010000803 DASTMD010000000
VERSION     DASTMD010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612147"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFQ54_11900"
     CDS             complement(<1..838)
                     /locus_tag="VFQ54_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165738.1"
                     /translation="MFDRWRSLRRGILSATTFAIVAILMLGNAGIDGVGAEVDATPWT
                     DPVDDMGDAGVDPDGESPAPTGTSVSSTFQAAEEPIVPVVSWAEPTTLTCATPSVPGV
                     VNGTLSLENVSQFYSLTVWASLPENSVAVGDEFEWTSLNAAYFTGYVSYQVDYPYSSQ
                     NQRVTVITIHYYLVGRSLRGEREITLERCAPSPTPTQKPIVVPTMQPTATERPSPTPR
                     PTSTPTATPTPTRTPSPTATATRTPSPTATPTRTPNVVATRTPTATRTATVRPTATKA
                     PTA"
     gene            complement(1047..1325)
                     /locus_tag="VFQ54_11905"
     CDS             complement(1047..1325)
                     /locus_tag="VFQ54_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0165739.1"
                     /translation="MLTKSVGDQRNLAQDHPETCVAVISLLTAREREVLGLIAEGFSD
                     RGIAESLRISPLTATTHVKRILHKLHVPSRSAAAAIAVRAELSARGRR"
     gene            1384..>1893
                     /locus_tag="VFQ54_11910"
     CDS             1384..>1893
                     /locus_tag="VFQ54_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165740.1"
                     /translation="MNAFYPKPTRPWSIRLRRGPHIDARRRPRQRHVREGNDRNEKRV
                     TRYRRQGLPNEPRHQVEADRHRKSLPISIICPVLMILRDGDYSIPGDERTGNLTRFSG
                     GNRLCRYHGAAAIERYASPNTHGNGREPWFPPVSDPLYCYGAGVDVAVGSGVSSRFEA
                     INELGEITKN"
BASE COUNT          346 a          589 c          609 g          349 t
ORIGIN      
        1 gggcggtggg agccttcgtc gcggtgggcc ggaccgtggc ggtccgcgtc gccgtgggcg
       61 tcctggtcgc gacgacgttc ggagttctgg tgggagtcgc cgttggagat ggcgtccgcg
      121 tcgcggtcgc ggtgggagaa ggagttcgtg tcggggttgg cgttgccgtc ggcgtggagg
      181 taggccgcgg cgttggcgac ggtcgctcgg tcgccgtcgg ctgcatcgtg ggaacgacga
      241 tcggtttctg cgtcggcgtc ggcgagggcg cacatcgctc gagcgtgatc tcccgctcgc
      301 ctctgagact tcttccaacc agatagtagt gaatcgtgat caccgtgacg cgttggttct
      361 gcgaactata gggatagtcc acctgatacg agacatagcc ggtgaaatac gcggcgttca
      421 gggatgtcca ctcgaactcg tcgccgaccg ccaccgagtt ctctgggagt gaggcccaga
      481 ccgtaaggga atagaactgg gagacgttct ccagcgagag cgtgccattc acgactcctg
      541 gcacgctcgg ggtcgcgcag gtgagcgtcg tcggctccgc ccatgagacg accgggacga
      601 tcggctcctc ggcggcctga aaggtggaac tgacgctcgt acccgtcggc gccggtgact
      661 cgccgtccgg atcgacaccg gcgtcgccca tatcgtcgac gggatccgtc cagggtgtgg
      721 cgtcaacctc ggcgccgacg ccgtcaattc cggcattgcc gagcatcagg atcgccacga
      781 tcgcgaacgt cgtcgcactc aggatgcccc ggcgaagcga tcgccatcga tcgaacacga
      841 tcccctccca acgattctgt catcgcgtgt tgcattatcg tgtgaccccg gtctgcaccg
      901 atcataccgg tagtcgacag ctttatgcaa tcctgtgaca gaaaaaacgg gacgggctga
      961 aacagcagcc cgtcccgctt cctggatttt cctgtcgtcg aaccgtcgag gggcacgatc
     1021 agtcgctgtg aggtgacctc cgccgatcag cgtcgccctc tggcgctcag ctcggcccgc
     1081 accgcgatcg ccgccgccgc cgagcgcgac ggcacgtgga gcttgtgaag gatgcgcttg
     1141 acatgggtcg tcgcggtgag ggggctgatg cggagcgact ccgcgatccc gcgatcggag
     1201 aagccctcgg cgatcaatcc gagcacttcg cgctcgcggg cagtcaggag cgagatgacg
     1261 gctacgcacg tctccggatg atcctgtgcc aggttccttt ggtcgccgac ggattttgta
     1321 agcatcatcg cctccaccac gagatcccca atggaagaac gttctccccg ccaccgatcg
     1381 aggatgaacg ccttctaccc caaacccacc cggccgtgga gcatccggct ccggcgcggt
     1441 ccccacatcg atgcgcgacg ccgcccacgc cagaggcacg tccgcgaagg caacgatcga
     1501 aacgagaaac gcgtcacgcg gtatcgccgc cagggtcttc cgaacgagcc tcgccatcaa
     1561 gttgaagccg atcggcatcg taaaagcctg ccgataagca ttatctgtcc cgtcttgatg
     1621 atactccgcg atggggatta ttcaatcccg ggtgatgaga gaacaggcaa cctgacccga
     1681 ttctccggcg ggaatcggtt gtgcaggtat cacggtgcag cggcaatcga gcgatacgca
     1741 tcgccgaaca cacacggaaa cgggcgggaa ccgtggttcc cgcccgtttc cgatcctctc
     1801 tattgctacg gtgccggcgt cgatgtcgcg gtcggctcgg gcgtctcgtc gaggttcgaa
     1861 gccataaatg aattgggtga aataaccaaa aac
//
LOCUS       DASTMD010000804         1827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2843456, whole genome shotgun sequence.
ACCESSION   DASTMD010000804 DASTMD010000000
VERSION     DASTMD010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2843456"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFQ54_11915"
     CDS             complement(<1..277)
                     /locus_tag="VFQ54_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165741.1"
                     /translation="MGATGYNLNHLQVAAKGASPVYKDVLYATTFQPTISQTSTKLKA
                     DGKNAVTAWSAADGGGSIGFGSAGTDLMALLTGGTASTSGSAGTLINR"
     gene            complement(281..922)
                     /locus_tag="VFQ54_11920"
     CDS             complement(281..922)
                     /locus_tag="VFQ54_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165742.1"
                     /translation="MTKTIEYTGGENPFADIAGFVGRAKSQYPGISDYDVNVIVKRTL
                     GLRMERHKQRVVTLFGADIMTEQLEDIPSWSLLEWRHRSRIASYPLHGGDMADEANFA
                     ESQKQLILRLDTEHVNRSVLISPRGLGNTYVWCAGNEWRHEVSDADYDLILATPARRF
                     YFRDPDVHGLFVEQRAYMSNPVLSRHSGTPDEFAAFMDDRTRKPQWQGASLKE"
     gene            complement(942..1136)
                     /locus_tag="VFQ54_11925"
     CDS             complement(942..1136)
                     /locus_tag="VFQ54_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165743.1"
                     /translation="MATKSTTTEETTPATPVAAPVPEQTGELADLGKKYVETGEMKPG
                     YDYRYVTIGGETKPVVTKRG"
     gene            complement(1150..1608)
                     /locus_tag="VFQ54_11930"
     CDS             complement(1150..1608)
                     /locus_tag="VFQ54_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165744.1"
                     /translation="MTRLAERVLTALLADSAIATLVDGRAFPYDIRIAGPSSQFPTVY
                     DAFGAIEGPTMLVVDGGAGRTMSGLLGAYDGRLVVWGLVDGATETGRDDLATLMTRSA
                     AVLIGWQDTESKALLTFGDRRGPIDDPPPDTGVLDRVTFRVGGVHAGIAG"
     gene            complement(1605..>1827)
                     /locus_tag="VFQ54_11935"
     CDS             complement(1605..>1827)
                     /locus_tag="VFQ54_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165745.1"
                     /translation="ARQGLFGTATSEGDVHTIILGGTMEYQQYLEGGTHNEDGTTRMA
                     PRPIIVPTAETVALDFFIDGGKVAERILG"
BASE COUNT          291 a          612 c          553 g          371 t
ORIGIN      
        1 ggcgattgat cagcgtcccg gctgacccgg acgtgctggc cgtacctcct gtgagcagtg
       61 ccatcagatc cgtaccggcg ctgccgaagc caatcgagcc tccaccgtcc gccgcgctcc
      121 atgcggtcac ggcattctta ccatcggcct tgagcttggt gctcgtctgg ctgatggtcg
      181 gctggaacgt ggtcgcgtag agcacgtcct tgtagaccgg tgacgcgccc tttgcggcca
      241 cctgcaggtg attgaggtta tatccggtcg ctcccatgac ttactccttg agactggcac
      301 cctgccactg aggcttccgg gtgcgatcgt ccatgaacgc cgcaaactcg tcgggcgttc
      361 cgctgtggcg actgagcacg ggattgctca tgtacgcccg ctgttcaaca aagaggccgt
      421 ggacgtccgg atcgcgaaag tagaagcggc gggccggtgt ggcgaggatc aagtcgtagt
      481 cggcatctga cacctcgtgg cgccactcgt ttccggcgca ccagacatag gtattgccga
      541 gccctcgcgg gctgatcagg acggacctgt tgacgtgctc cgtatcgagc cgaaggatga
      601 gctgcttctg gctttcggcg aagttcgcct cgtcagccat gtcgccccca tggaggggat
      661 agctcgcgat ccgcgaccgg tgccgccatt ccagcagcga ccaactcggg atgtcctcga
      721 gttgctccgt catgatgtcg gcgccgaaca gcgtgacgac acgttgcttg tgtcgctcca
      781 tgcggagccc aagcgttcgc ttgacgatga cgttgacgtc gtaatcggag attcccggat
      841 actgcgactt cgcacgcccc acaaagcccg cgatgtcggc aaacgggttc tcgcctccgg
      901 tgtattcgat cgtcttcgtc atcgcgtccg tcctgtctcg tctagccgcg cttggtcacg
      961 accggcttgg tctcgccgcc gatcgtcaca tagcgatagt catagcccgg cttcatctcg
     1021 ccggtctcga catacttctt gccgaggtcc gccagttcgc cggtctgttc cgggacgggc
     1081 gccgcgaccg gagtcgccgg agtcgtctct tcagtcgtcg tgctcttcgt agccatgatg
     1141 tagctctttc tatccggcga tgccggcgtg tacgcctcca acccgaaagg tgacccgatc
     1201 gagaacgccg gtatcgggcg gtggatcgtc aatcggcccg cgccgatcgc caaaggtcag
     1261 gagagccttc gattcggtgt cctgccagcc aatcaggacg gcggctgacc gggtcatgag
     1321 cgttgcgaga tcgtctcgtc ccgtctccgt cgccccgtcc acaagccccc acaccaccag
     1381 acgcccgtcg tacgcaccga gcagtccgct catcgttcgc cccgccccgc catcgaccac
     1441 cagcatcgtc ggcccctcga tcgccccgaa cgcgtcgtag acggtcggga actgcgagct
     1501 cggtccggcg atccggatgt cgtacgggaa cgctcgtcca tccacgagcg tggcgatggc
     1561 gctgtccgcg agcagggccg tgagcactcg ctcggcaagc cgggtcatcc gagaatcctc
     1621 tcggccacct tgccgccgtc gatgaagaag tcgagagcga cggtctctgc ggtcggcacg
     1681 atgattggtc gcggcgccat cctcgttgtg ccgtcctcgt tatgcgtccc accctcgaga
     1741 tactgctgat actccatcgt gccgccgagg atgatcgtgt gcacgtcacc ctcgctggtc
     1801 gccgttccga agagcccttg acgggca
//
LOCUS       DASTMD010000805         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_162245, whole genome shotgun sequence.
ACCESSION   DASTMD010000805 DASTMD010000000
VERSION     DASTMD010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162245"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..376)
                     /locus_tag="VFQ54_11940"
     CDS             complement(131..376)
                     /locus_tag="VFQ54_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU0165746.1"
                     /translation="MVGGVIGWIVVGIAAGWLAGVVTRTNRNIIGDLVLGLIGAFVAG
                     LITNGVVGGDAGIISSIIVAAIFAAILVLIKNAVVGR"
     gene            complement(500..1591)
                     /locus_tag="VFQ54_11945"
     CDS             complement(500..1591)
                     /locus_tag="VFQ54_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165747.1"
                     /translation="MPLGGDESVDYLVFGVGFGSSLVLLGWLLRQFGPTFRYRTKGDE
                     SSEVLSAEELIARQDWRTFCMAVGAILSVSGALMLFVTVVTLLLRPADSTGSVIVGVV
                     FVLTLLGIAGWIALFVSQHENIGGFADLARMNWKPRIPRLASGSKDAADGDHDDPDAD
                     LDQDDFGFPAAPASPPVPARTPTAQDPSPTPTRRIVNPARPSNRPIAATRPKPGTQAT
                     PRPPQTSSAASQPATNAPASQNEDENLLAGVRFSRPRSATPNERQQATSSPAPREQTP
                     QPARSEPQSQPPVSPSPRQRTPVVGDDVVSSVTMKDAGEDDPSATSEMPRSRQAPARE
                     TSSSEASSREAALANLRLRRAGRPDSRDS"
     gene            complement(1775..>2314)
                     /locus_tag="VFQ54_11950"
     CDS             complement(1775..>2314)
                     /locus_tag="VFQ54_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0165748.1"
                     /translation="GLDELWGEKGWTPRERIWGRPTLDMNGIWGGFQGEGVKTVTPNQ
                     AHLKVTCRLVPDQDPAEIVELIRKHVEAHRPPGATVEVVGGKGGADPYVVDRSNPVHI
                     AAGKVLKDLFGTEPVLERAGGTIPATAMFKKLLGLDTIVFAWSIPGAGAHAPNEWYRL
                     EDYTRGRTGFALLIEELAR"
BASE COUNT          391 a          735 c          768 g          420 t
ORIGIN      
        1 gagcgcctga cgcccgtttc gcgcgccctg aatgccacgc ggtaaatctc cctccgcctc
       61 ggatggtaaa ggggagatcg cctcgaggcg atctcccctt cttcgcgtcg ttggttcgct
      121 ggtggtctcg ctatcgtccg acgacggcgt tcttgatcag aaccaggatc gcggcgaaga
      181 tcgctgccac gataatgctg gaaatgatcc cggcatcgcc gccaaccacg ccgttcgtga
      241 tcaacccggc aacgaaggca ccgatcagtc cgagaacaag atctccgatg atgttgcgat
      301 tcgtgcgcgt gacgacgcct gcgagccaac ccgcggcgat acccacgacg atccacccaa
      361 tgactccacc gaccatacgt cgtctggctc ctttacatgg cagggcagcg tcatgtgctc
      421 catgcctcat tgcgaaaggg gttcgcggta tccctaacgc aatgcaggtg ccacatggtc
      481 acgggcgggc gcgggatgct caggagtcgc gtgagtccgg tcggccggcc cggcggaggc
      541 ggagattggc gagcgcggcc tcgcgtgacg atgcctcgct cgaggatgtc tcccgggccg
      601 gtgcctgacg cgagcgaggc atctcgctgg tcgcggacgg atcgtcttca ccggcgtctt
      661 tcatcgtgac gctggaaacc acatcgtcac cgacgaccgg cgtgcgttgt cgtggtgacg
      721 gcgataccgg aggctgggac tgaggttctg accgggcggg ctgtggcgtc tgctcccggg
      781 gtgccggcga actcgttgcc tgctgtcgct cgttcggcgt cgccgatctt ggccgcgaga
      841 aacgaacccc ggcgagaagg ttctcatcct cattctgtga cgctggtgcg ttcgtcgcgg
      901 gttggctggc ggccgacgag gtctggggcg gtcgtggtgt cgcctgggtg ccgggtttcg
      961 gacgggtggc ggcgattggc cgattcgatg gccgcgcggg attcacgatc cggcgtgtcg
     1021 gggttggcga cgggtcctgc gcggttggcg ttcgtgctgg aaccggtggg gaagccggag
     1081 ccgccgggaa gccgaagtca tcctggtcga ggtcggcgtc cgggtcatcg tgatccccgt
     1141 ctgcggcgtc cttgctgccc gaggccaggc ggggaatccg gggcttccag ttcatgcgag
     1201 cgagatcggc gaagccgccg atgttctcgt gctggctgac gaagagggcg atccagcccg
     1261 cgatgccgag cagtgtcagg acgaagacga cgccgacgat caccgatccg gtgctgtctg
     1321 ccgggcggag caacagggtg acgaccgtga cgaagagcat cagcgcgcca ctgacggaga
     1381 ggatcgctcc cacggccatg cagaacgtgc gccagtcctg ccgcgcgatc aactcctcgg
     1441 cgctgaggac ctcggaagat tcatcgcctt tcgtccgata gcgaaatgtc ggaccgaact
     1501 gccgtagcag ccatccgagg agcaccagac tcgagccgaa gccgacgccg aacaccagat
     1561 agtccacgga ctcatcaccc ccgagcggca ttcccgctgc ctcgtacgca acctttgggg
     1621 aaacctactg tgtttcgccg tcgatgtgca atatacagaa gcgaaaatca ccgactttgc
     1681 ccggccggaa gtatctcacg ggtatcagtg cgaaaaaagg agacccgcat gccgggtctc
     1741 cctctgccgt gtcgtgcgat cgattgtcga gatgctatcg ggcgagttcc tcgatgagca
     1801 gcgcgaaacc ggtgcggccc cgcgtgtaat cctcgagccg gtaccactcg ttcggagcat
     1861 gcgcgccggc acccgggata ctccaggcga agacgatcgt gtcgaggccg agcaacttct
     1921 tgaacatcgc ggtcgccggg atcgtgccgc ccgcccgttc gaggacgggc tcggtgccga
     1981 agagatcctt gaggaccttg cccgcggcga tgtggaccgg attcgagcga tcgacgacgt
     2041 agggatcggc gccacccttg ccaccgacga cctcgaccgt ggcgcccggc ggtcgatgcg
     2101 cctcgacgtg cttgcgaatc agttcaacga tctccgccgg atcctgatcc gggacgaggc
     2161 ggcaggttac cttgaggtgc gcctggttcg gcgtcaccgt cttgacgcct tctccctgga
     2221 agccgcccca gatcccgttc atgtcgagcg tcggacggcc ccagatgcgc tcgcgcggcg
     2281 tccagccttt ctcaccccag agttcatcga gacc
//
LOCUS       DASTMD010000806         4280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1684376, whole genome shotgun sequence.
ACCESSION   DASTMD010000806 DASTMD010000000
VERSION     DASTMD010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684376"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="VFQ54_11955"
     CDS             <1..1462
                     /locus_tag="VFQ54_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165749.1"
                     /translation="YHRETVLGTIEHAATFRDSWLRWQWQALAATVESGKNGAFVLPP
                     LEHQTDPAAALDLLRMMDEAEVRVDTLQNAATDTGGARHPRGSFVIRLDQPFAGWPKA
                     LLPPTIYPNPDASSRPYDTTTHSLGVHMGVEASLVEHEEDWSLAPVAIESLDGISSLD
                     ERRTGQGRWMAIDPRSHDAIRVVTAAMTQGGRVHRLHRSHFSEGRIFDPGTWLISDVP
                     TESLHQEADRLGVRSYSLTPVSEGLLAQRQPRIGVHLADWQNATDAGWTRLILDEAGV
                     PYEILADRDIRDSDLDQFDVILLPHATAAQLVTGHKETEYPEEFAGGLRESGFTQLRQ
                     FVEQGGQLVAIDGSAQALIEQLGLPVELPLAKLTDAEFFAPGAAIRTVVDTTHPLGWG
                     LPAILPALQSGKNALRANPESSASAPLKFVAEDLVASGWLYGGEQLAGLDAVIDLPHG
                     AGFFTLIAIRPQFRAQTYVSFNLLFNALYRSGLSQA"
     gene            1510..1944
                     /locus_tag="VFQ54_11960"
     CDS             1510..1944
                     /locus_tag="VFQ54_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0165750.1"
                     /translation="MLVVTDDDSLAGFLNEGLPLGGFWASWIASGLQALEVFRLRQFD
                     LVLIDAGLRSFDALELMRRLRGTSSRSSDRTPRTKAPFVVVSEEPEPITHDEAATLGV
                     AAVMSAPIELEEIVQRLHEVFAAWRQDNPDTPLADSIANLSA"
     gene            complement(1890..2615)
                     /gene="phoU"
                     /locus_tag="VFQ54_11965"
     CDS             complement(1890..2615)
                     /gene="phoU"
                     /locus_tag="VFQ54_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU0165751.1"
                     /translation="MSTRPAYDHMIQNVRDDVVSMASMVDKAIARAIDALRTQDVALA
                     RQVKDGDKSINAYRWRAEEHALMIIATQGPMAGDLRLVAAALQIFTDLERMGDHAAGI
                     AKIVIQTADEPMLKPLVDIPRMAMEARSMLNDAITSFIELDEDRARKVAIRDDEIDIL
                     YDQVYRELLTYMMADPSTINRATRLLWAAHNIERIGDRVTNICERVVFTTSGEFVSMD
                     GGRKKRQAERLAMESASGVSGLS"
     gene            complement(2728..3567)
                     /gene="pstB"
                     /locus_tag="VFQ54_11970"
     CDS             complement(2728..3567)
                     /gene="pstB"
                     /locus_tag="VFQ54_11970"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEU0165752.1"
                     /translation="MATEAKDVRIPSVTAGMQAAEDKTPTEKILDLIDVNIFYGAFHA
                     VNNVNLAIPKNRITAFIGPSGCGKSTVLRSINRMNDLVPSARVDGQILYHGQDLNSKK
                     VDPVEVRRRIGMVFQRPNPFPKSIFDNVAWGAKINGFKGNVSELVERCLRQAALWDEV
                     KDKLKESGLGLSGGQQQRLCIARALATSPEIILLDEPASALDPISTLKIEELLRELQP
                     NYTIVIVTHNMQQAARVSDQTVFYSLNDQRQGTLVEAGPTQQIFTNPNDPRTEAYITG
                     RFG"
BASE COUNT          734 a         1379 c         1329 g          838 t
ORIGIN      
        1 ctatcaccgc gagaccgtgc tcggcacgat cgagcatgcc gcgacgttcc gcgattcctg
       61 gctccgctgg cagtggcagg cgctggcggc gaccgtcgaa tcgggaaaga acggggcgtt
      121 tgtcctcccg ccgctggagc atcagacgga tccggccgcc gcactcgatc tcctgcggat
      181 gatggacgag gcagaggtga gggtcgacac gcttcagaac gcggcgacgg ataccggggg
      241 cgccaggcat ccgcggggct cgttcgtgat ccgtctcgat caaccgttcg ccggctggcc
      301 gaaggcgctt cttccgccga cgatctatcc caatcccgac gcctcctcgc ggccatatga
      361 caccacgacg cattcgctcg gcgttcacat gggcgttgaa gcctctctcg tcgagcatga
      421 ggaagactgg tcgctggcgc cggtcgcgat cgagtcgctc gatgggatat cgtcactcga
      481 cgagcgtcgc accgggcaag gacgctggat ggcgatcgat ccacggagtc acgatgcgat
      541 ccgcgtcgtg acggcagcga tgacccaggg tgggcgcgtg catcggctgc acagatccca
      601 cttcagcgaa ggacggatct tcgatcccgg gacctggctg atctccgatg tcccgaccga
      661 atcgctgcat caggaggccg atcgcctcgg tgtccgatcg tactcactca cgcccgtatc
      721 cgaaggtctc ctcgcgcagc gacagccccg catcggtgtt cacctcgccg actggcagaa
      781 tgccaccgac gcgggttgga cccggctcat cctcgacgag gccggagtcc cctacgagat
      841 cctcgcggat cgggacatcc gcgacagcga tcttgaccag ttcgacgtga tcctcctccc
      901 gcacgccacc gccgcgcagc tcgtcaccgg ccacaaggaa accgagtatc ccgaggagtt
      961 cgccggcggc ctccgcgaat ccggcttcac gcagctgcgg cagtttgtcg agcagggcgg
     1021 tcagctcgtc gcgatcgacg gatcggcaca ggcgctgatc gagcagctgg ggctgcccgt
     1081 cgagttgcca ctcgcgaagt tgacggacgc cgaattcttc gccccgggcg cggcgatccg
     1141 gaccgtcgtt gatacgacgc atccgcttgg atggggattg ccggcgattc ttccggcgct
     1201 tcaaagcggc aagaacgcgc ttcgcgcgaa cccggaatcc tcggcgtcgg cacccttgaa
     1261 gttcgtggcc gaggacctgg tggcatcggg atggctgtac ggcggggagc aactcgccgg
     1321 tctcgatgcg gtgatcgatc ttccgcatgg cgccggattc ttcaccctga tcgccatccg
     1381 gccccagttc cgcgcacaga cctatgtttc cttcaacctg cttttcaacg cactctatcg
     1441 atcgggtctc agccaggcat gaccgaacgc ccgcgtgtcg ccgaacgcaa acagcatgag
     1501 cgtccccacg tgctggtggt cacggacgat gattcgctgg ccgggttcct caacgagggg
     1561 ctgccgctcg gcggcttctg ggcatcctgg atcgcgagcg gattgcaggc tctcgaagtc
     1621 tttcgactcc gccagttcga cctcgtcctg atcgacgccg gtctcaggag tttcgacgcc
     1681 ctcgaactga tgcgacggct gcgcggaacg tcatcccgca gcagtgaccg cacgccccgt
     1741 acgaaggcgc cgttcgtcgt cgtctcggaa gagccggagc cgatcacgca tgacgaagcc
     1801 gcgacactcg gtgtcgcggc cgtcatgtcg gcgcccattg aactcgagga gatcgtgcag
     1861 cgactgcacg aggtcttcgc ggcgtggcgt caggacaacc cggacacccc gctggcggat
     1921 tccatcgcca acctctcggc ctgacgcttc ttccgtcccc catccatcga gacgaactcg
     1981 cccgaggtgg tgaagaccac gcgttcgcag atgttcgtga cgcgatcgcc gatccgctcg
     2041 atgttgtggg cggcccagag caaccgggtc gcacggttga tcgtgctggg gtcggccatc
     2101 atgtaggtca gcaactcgcg atagacctga tcgtagagga tgtcgatttc gtcgtcacga
     2161 atggcgacct tgcgggcgcg atcctcatcc agctcgatga aggacgtgat cgcgtcgttg
     2221 agcatgctcc gcgcttccat cgccatccgg gggatatcga cgaggggctt cagcatcggc
     2281 tcgtccgccg tctggatcac gatcttcgcg ataccggcgg catggtcgcc catccgctcg
     2341 agatcggtga agatctgaag cgccgcggcg acgaggcgga gatcgcccgc catcggtccc
     2401 tgcgtcgcga tgatcatcag cgcatgctct tcggcgcgcc aacgataggc gttgatcgac
     2461 ttgtctccat ccttgacctg gcgcgcgagc gcgacatcct gggtgcggag ggcgtcgatg
     2521 gcgcgagcga tggccttgtc gaccatgctc gccatcgaga caacatcgtc acgaacgttc
     2581 tgaatcatgt ggtcgtatgc agggcgcgtt gacatggtca tggttgctcc caatgggcgg
     2641 tccgggcacc acgcgctgcc ggctggcatg gcgcgtcatt cgtcagatat tctccgaaca
     2701 acgcgatttc catgccccgg caacccgcta tccgaatcga cccgtgatgt aggcttccgt
     2761 ccgcggatcg ttcgggttcg tgaagatctg ttgcgtcggt cccgcctcga cgagcgtgcc
     2821 ctggcgctgg tcgttcagcg agtagaacac cgtctggtcg gagacgcgcg ccgcctgctg
     2881 catgttgtgc gtgacgatca cgatcgtgta gttcggctgc aactcgcgga gcagttcctc
     2941 gatcttgagc gtcgagatcg ggtcgagcgc ggaagccggc tcgtcgagca ggatgatttc
     3001 cgggcttgtg gcgagcgcgc gcgcgatgca gaggcgctgc tgctggccac cggagaggcc
     3061 gaggccgctc tccttgagct tgtccttcac ttcatcccag agtgcggcct gacgcaggca
     3121 gcgctccacg agctcggaaa cattgccctt gaagccgttg atcttcgcgc cccaggcgac
     3181 attgtcgaag atgctcttcg ggaacggatt cggtcgctgg aagaccatcc cgatacggcg
     3241 acgcacctcg accggatcga ccttcttcga gttcaggtcc tgaccgtgat agaggatctg
     3301 cccgtcaacg cgtgcgctcg gcacgaggtc gttcatccgg ttgatgctgc gcagcacggt
     3361 gctcttgccg caaccggagg gcccgatgaa ggccgtgata cggttcttcg ggatcgccag
     3421 attgacgttg ttcaccgcgt ggaacgcgcc gtagaagatg ttcacgtcga tcagatcgag
     3481 gatcttctcg gttggcgtct tgtcctcagc cgcctgcatg ccggccgtca cgctgggaat
     3541 gcgaacgtct ttcgcctcgg ttgccatcgc cctaccacct gttcttcttg ctgaaatgct
     3601 gccgcgatgc gatcgccgtg gacgtcacga ccagacgatt gaccgattgc ggcggccgcg
     3661 agatgtccga agaccgttgc cgttgtgccg tcctgctgaa atctcctgcc acaccgtcac
     3721 gaacggttcc ttgaaaccgt cctggcaccg ggaccggtga ccgcgatgat gtcgaggaga
     3781 aatctcctgt cccaattaca atccagatcg atcgcgggga atgctgggtt catcccggct
     3841 ctggcggcaa ttttgtgaac acgtgcccga atcagcacca agcccgatcg cctgggtcga
     3901 gtcctttaca tcctccaagc ccgatgcgga ccgctgcagt ctcgggatct gccgcagttt
     3961 gcctcaaagg tcatcccgca tcgtatgcgc tcacgacttg tttgaggata ttcgggacgt
     4021 tccactcaat tgcaaagaat ccgtcaacga atgccgtaat ttcctcgacc agttctcccg
     4081 agcagtcggt acgcgctgat ttgcgttccg gcaaccagcg tcgatcggct atcggagatg
     4141 aactcaggcg atgacagcca gctcaggttc gtcgcgtccg gtttccgggg cgacgacgaa
     4201 gcggtatccg ctgccgtaga ccgtccggat gcaatcgtgt gcaagcccgg cggcctgcag
     4261 cttgcctcgt agccagctga
//
LOCUS       DASTMD010000807         1772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1593699, whole genome shotgun sequence.
ACCESSION   DASTMD010000807 DASTMD010000000
VERSION     DASTMD010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593699"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFQ54_11975"
     CDS             <1..233
                     /locus_tag="VFQ54_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165753.1"
                     /translation="EEIAEGDYAQLESDFGDRESAIKAVVLLEQPFIKDPKRTIGELV
                     KEAISKLGENIVVRRFSRFELGENLDGAAETA"
     gene            321..1061
                     /gene="pyrH"
                     /locus_tag="VFQ54_11980"
     CDS             321..1061
                     /gene="pyrH"
                     /locus_tag="VFQ54_11980"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU0165754.1"
                     /translation="MPNRFRRVLLKLSGEALMGDEHFGIDPAVSLRLADEIRSIHATG
                     VEIGIVIGGGNIWRGLGAASKGMDRATADYMGMVATVLNGLSLQDALEKEDVPTRVLT
                     ALAINQVAEPYIRRRALRHMEKGRVVILVAGTGNPFFTTDTAAALRALELDADVLLMA
                     KNKVDGVYSDDPRLNPDAERYHHITYQEALERNLKVMDASAMALCRDNDLPVIVFDVT
                     QHGVIQRIIEGENAGTLVSSSVTSIPNS"
     gene            1132..1689
                     /gene="frr"
                     /locus_tag="VFQ54_11985"
     CDS             1132..1689
                     /gene="frr"
                     /locus_tag="VFQ54_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU0165755.1"
                     /translation="MISDVLKNAEQRMTGAIEALHKNLNAVRTGRANPALVDRIMVEF
                     YGTTMPLNQLANISAPESRMIVIQPFDRGSMSAIERALLTSDLGVNPSNDGQVIRISI
                     PALTEDRRKQMVKTVHSNVEEGKVALRNVRRDAVQQARDLLAKKEISEDDERKATQDF
                     DALTKKYVDEAEKVGKAKEQEVLEV"
BASE COUNT          367 a          569 c          535 g          301 t
ORIGIN      
        1 tcgaggagat cgccgagggc gattacgcgc agctcgagag cgacttcggc gaccgcgagt
       61 ccgcgatcaa ggcggtcgtc ctcctggagc agccgttcat caaggatccg aagcgcacga
      121 tcggcgagct cgtgaaggaa gcgatcagca agctcggcga gaacatcgtc gtccgccgct
      181 tctcccggtt cgaactcggc gagaacctcg acggcgccgc cgaaaccgcc tagtcctcac
      241 gagaaccgcc gcccgatcgt ggggcggcgg ttctcctttc ctccctcttc gctgagcggt
      301 cttcgaaagg atccccggaa atgccaaatc ggttccgccg tgtccttctg aagctgagtg
      361 gcgaggcatt gatgggggac gagcatttcg gcatcgatcc cgcggtttcg ctccgtctcg
      421 ccgacgagat tcgctccatt cacgcgacgg gcgtcgaaat cgggatcgtg atcggcggcg
      481 gcaatatctg gcggggtctc ggcgccgcga gcaaggggat ggaccgcgcg accgccgact
      541 acatgggcat ggtcgcgacc gtcctcaacg gcctgtcgct ccaggacgca ctcgagaagg
      601 aagacgtccc cacccgcgtc ctgaccgcgc tcgcgatcaa ccaggtcgcg gagccgtata
      661 tccgccggcg cgccctgcga cacatggaaa agggtcgcgt cgtgatcctc gtcgccggaa
      721 ccggcaatcc cttcttcacc accgacacgg cggcggccct ccgggcactc gaacttgacg
      781 ccgacgtcct cctgatggcg aagaacaagg tcgatggcgt ctattcagac gatccccgcc
      841 tgaatcccga cgctgaacgc taccatcaca tcacctacca ggaagctctt gagcgaaacc
      901 tcaaagtaat ggatgcttcg gcgatggcgc tctgccgcga caacgacctg cccgtgatcg
      961 tcttcgacgt cacgcagcat ggcgtgatcc agcgaatcat cgaaggtgag aacgccggca
     1021 ctttggtatc ctcttccgta acatctattc ccaattcctg aacgccgcga tcgccgcgcc
     1081 aatgcgggtg gatcccagtt ggaatccggg ctcgaaagga tgattgtcga catgatttca
     1141 gatgttctga agaacgccga gcaacgcatg acgggcgcga ttgaggcgct gcacaagaac
     1201 ctcaatgcag ttcgcacggg tcgggcaaat cccgcgctcg tcgaccgcat catggtcgag
     1261 ttctacggga cgacgatgcc cctcaaccag ctcgccaata tctcggcgcc ggaatcacgc
     1321 atgatcgtga tccagccgtt cgaccgcggc agcatgagcg cgatcgaacg ggcgctcctt
     1381 acgtccgacc tcggtgtcaa tccgagcaac gacggccagg tgatccgcat ctcgatcccg
     1441 gcgttgaccg aggaccgtcg caagcagatg gtcaagaccg tgcactcgaa cgtcgaggag
     1501 ggaaaggttg cgctgcgcaa cgtgcgccgc gacgccgtcc agcaggccag ggatcttctt
     1561 gcgaagaagg agatctccga ggatgatgag cggaaggcca cccaggactt cgatgccttg
     1621 acgaagaaat acgtcgacga ggccgagaag gtgggcaagg ccaaggagca ggaagtgctc
     1681 gaggtctgat catggaacac acggatcggt cttcccccat atccgagagt cccgtactcg
     1741 cccaacaccc ggagcgtacg ccgcgccacg tc
//
LOCUS       DASTMD010000808         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2397467, whole genome shotgun sequence.
ACCESSION   DASTMD010000808 DASTMD010000000
VERSION     DASTMD010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2397467"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(391..1191)
                     /locus_tag="VFQ54_11990"
     CDS             complement(391..1191)
                     /locus_tag="VFQ54_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEU0165756.1"
                     /translation="MYRLDDELTTPVERGIATVAQALGDEGRVRILVALLDGRALPAT
                     RLAMEAGVAPSTASVHLRKLLDTGLLKEVPSPVNRGRYRFYAIAGEEVAALCERFLAL
                     IPHAPVRSLQAGTRDAAMRSARTCYDHLAGRLAIRLMDAMLTQGMIASHDGSFAIGAT
                     GEARFARFGIDLGELRARKRPLIRHCLDWSERCPHLSGGLGAALLQQIEERGWIERSL
                     TDRSATITTAGKLGFQTWFDLNVDDLDMGHADHLRDLANDDAVREVIP"
     gene            1517..>2285
                     /locus_tag="VFQ54_11995"
     CDS             1517..>2285
                     /locus_tag="VFQ54_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0165757.1"
                     /translation="MTKGQSGPFQQLFDALKRGEIDRRRFIQRSTALGMGAAVALYCA
                     DSVTLAQTPGASPAAAGADARPATGTEGQTRGAGGELKVLQWQAPSHLSGHLATGDKD
                     SLASTPISEPLLNRMPDGSLTPCLATQVPTVENGQLAADLTNVTYTLKQGVLWSDGTP
                     FTANDVRFTWQWVMNPDNSAVTVGLFQPIKDIEVVDDHTVKVIFSAANPTWSDPHTGS
                     GGGVIYPRHILENGGQKASDAFGLKPIGTGPYVVDSFS"
BASE COUNT          421 a          777 c          712 g          375 t
ORIGIN      
        1 cctccgccgc ctgactgttg ccgagcagcc gatagcagta gcggaagatc gacgtcacgt
       61 atcgatcgta gagtggggcg aaggcggcgc ggtcgtactg ggcccgtacg acgagtccgg
      121 cttcgtcatc ctccggcatc gaatccccca cccgaagtcc caggaaaagt ggtcttcccg
      181 atcgcgtgct gatgcgatca gggtagcgcg cgtacaccct gaagaacggg ggttccggaa
      241 aaaggttctg ggtattccct gtgacgatgc cattggaacg acgcgcccgc attcccgtcc
      301 ccacggcgac aagcgccgtg gggacgggaa tggtagcgtg tcttccagaa cgcacgttga
      361 aggcaaacgc cagagccaca ccaggtcgcc ctatgggatt acctcgcgca cggcgtcatc
      421 gttcgccaga tcgcgcaagt gatcggcgtg gcccatgtcg aggtcgtcga cgttcaggtc
      481 gaaccaggtc tggaatccga gcttgcccgc tgtggtgatc gtcgccgagc ggtcggtcag
      541 cgaacgctcg atccagccac gctcctcgat ctgctgcagc agcgccgcac ccagaccgcc
      601 ggagagatgc gggcaccgct cgctccagtc caggcagtgt cggatcaggg ggcgctttct
      661 ggcgcgcagc tccccgaggt cgatgccgaa acgcgcgaat cgcgcttcac ccgtcgcgcc
      721 gatcgcgaag ctcccgtcgt gcgacgcgat catcccctgc gtcagcatgg catccatcag
      781 ccggatcgcg aggcgaccgg cgagatggtc gtagcaggtg cgcgccgacc gcatcgccgc
      841 atcgcgcgtt cccgcctgca acgaacgcac cggagcgtgc gggatgagcg ccaggaaacg
      901 ctcgcagagc gccgcgactt cctcgccggc gatcgcgtag aagcgatatc gcccgcgatt
      961 cactggcgat ggaacctcct tcaggagacc ggtatcgagc agcttccgga gatgcacgct
     1021 cgccgtcgac ggcgcgacac ccgcctccat cgcgagacgc gtcgccggca gggcgcgtcc
     1081 gtccagcagt gcgaccagaa tccgcacccg cccctcgtca ccgagcgcct gcgcgaccgt
     1141 ggcgatcccg cgttccacgg gggttgtcaa ctcatcgtcc aaccgataca tcgacatggc
     1201 tcctttcgtc tcgaccagac taccgcccgg cgggtatcca tgcttcgtcg ctcgacgaag
     1261 catggacgaa acacgcgatc ggccgtaggg cgtttgatcc cgcgttatgg tcaatatgac
     1321 caatggatga cgctttttcg gtgggatgct cccggccagg gcggtacact ggtttcaatg
     1381 tgtcgaagtg atgtcacgat tctcgatata tgagcgccat tttctgtagt ggttgcatga
     1441 agatctgcat cgcgacgcgc atctgccgtt caacaggttt cagcgacagc gctgaaaacg
     1501 aaggggactc tccaccatga caaagggcca gtccggaccg ttccagcagc tcttcgacgc
     1561 cttgaaacgc ggcgagatcg accgtcgccg gttcatccag cgctcgaccg cgctcggcat
     1621 gggcgccgcc gtcgccctct attgcgcgga cagcgtgacc ctcgcgcaga ccccgggcgc
     1681 gtcgccggcc gcagccggag ccgacgctcg cccggccacg ggcaccgagg gccagacccg
     1741 cggcgcaggt ggcgaactga aggtcctcca gtggcaggcg ccgagccatc tcagcgggca
     1801 cctcgcgacg ggtgacaagg acagcctcgc cagcacgccg atctccgagc cactcctcaa
     1861 ccggatgccg gacggatcgc tcacaccctg tctcgcgacg caggtgccga ccgtcgagaa
     1921 cggccagctc gccgccgatc tcaccaatgt cacctacacc ctgaagcagg gcgtgctctg
     1981 gagcgatggc accccgttca cggcgaatga cgtccgtttc acctggcaat gggtgatgaa
     2041 cccggacaac tcggccgtca cggtcgggct cttccagccg atcaaggaca tcgaggtcgt
     2101 cgacgatcac accgtgaagg tgatcttctc ggcggcgaac ccgacctgga gcgatcccca
     2161 cacgggatca ggcggcggcg tgatctatcc gcggcacatc ctcgagaacg gcggacagaa
     2221 ggccagcgac gcgttcggcc tgaagccgat cggcaccggg ccctacgtcg tcgattcgtt
     2281 ctcgc
//
LOCUS       DASTMD010000809         3986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_344214, whole genome shotgun sequence.
ACCESSION   DASTMD010000809 DASTMD010000000
VERSION     DASTMD010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344214"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VFQ54_12000"
     CDS             <1..844
                     /locus_tag="VFQ54_12000"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEU0165758.1"
                     /translation="ELAAAAYEVGFDFYGITDHNRAQSPVEFVPSGRGWPVLVPGVEV
                     TTYAGHFNVWGTDTWYDFRDPTPAGIQAAIDAARADGGFVSLNHPKPFGPPWEYPELT
                     GFDAIEPWNGWWNRLNDVSTRYWQERLTTGERVCGLGGSDMHYLGISGDADNPLSPAQ
                     MGMPTLWIQTDSPLSATSILEALRAGRSFISESPTGPQIFVQYDADLLRLAIDIVGAA
                     GDALVVVADAGIVFAGAIASDSDAFSWDGDVLASLFNGSRFLRAEIHRLGGGIRALSQ
                     PIWL"
     gene            complement(1203..1604)
                     /locus_tag="VFQ54_12005"
     CDS             complement(1203..1604)
                     /locus_tag="VFQ54_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165759.1"
                     /translation="MIEAGQEIIGRIREAAMVAVQELIPGNRSEIGYESVMAVLRAHP
                     TLAAQIAAVTEHAERTYPGATAVLEAKQYDDWDPPLVLTITIPSVSIDAYMDDFGSFY
                     KWLDADAGRVDESLVGVFPAYGGPVDEDARE"
     gene            complement(1636..2076)
                     /locus_tag="VFQ54_12010"
     CDS             complement(1636..2076)
                     /locus_tag="VFQ54_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165760.1"
                     /translation="MSDARIAIAERIEAIAQWRRDREQQDMLGLGPDAAMRSRRSAKG
                     LDELAALVRTLAPDDERLRRLREVAFHGEVFDPGATLLNELGRFRFHDPEVTVESFLE
                     RMIEFAERDEREMGEFGGPQIPGDNPWRANWILQVQTDDEDYDA"
     gene            2188..3147
                     /locus_tag="VFQ54_12015"
     CDS             2188..3147
                     /locus_tag="VFQ54_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0165761.1"
                     /translation="MEGESRVQSNTPGIAIETSRPVDAYLSLALAMAIVGSSVVTGKY
                     ITDGMPIFLALLLRFAGAVVVMVAWLGPAGRIRAHLILSRRDHTVIAIQALAGSVAFN
                     VLLLYGLRETTAAASGVITSATPVMIAIISFLLGERLRGRVWAGVLLTMAGVATVNVF
                     GVDGGTDHAANAVLGAILVLGAVVGESLYTILAKMVSPGVSPTTMALWSIIYSTMMFL
                     PLGLWQMRGFDPSAVPTSAWLSVAYSAVIVTVAAFVLWFRGLKQVPATTAAPFTGVIP
                     IVAVILAAILLGEPIGWPHLVGIVCVIGGILLITRTPSSQEIA"
     gene            complement(3151..3789)
                     /locus_tag="VFQ54_12020"
     CDS             complement(3151..3789)
                     /locus_tag="VFQ54_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165762.1"
                     /translation="MDETTHIFSPKVLDRAFTMELSDVDFTDYPPIATSVDAAMTEAA
                     RDALLVDFSRGGEFIRIDKAEIGLVLSEYPQIRGWLQTLNQLLARSRMNFGYRTFDEV
                     AAFVGNGMHNFSDPTIEAVFDRAVLMKVLPKFSGSASRLERPLLDFLAWCVHPVRIPQ
                     AEIRDAAEKLGDGGWLESNHEVFSDDMVLPVTGKRVQRMLDTLAVEGFASFG"
BASE COUNT          721 a         1312 c         1168 g          785 t
ORIGIN      
        1 cgaactcgcc gcggctgcct acgaagtcgg tttcgacttc tacgggatca ccgaccacaa
       61 ccgcgcccag tccccggtcg agttcgtccc cagtggaaga ggttggcccg tcctcgtccc
      121 gggcgtcgag gtaacgacgt acgcgggtca cttcaacgtc tggggcacgg atacgtggta
      181 cgacttccgg gatccgacgc ccgcaggaat ccaggcggcg atcgacgcgg cgcgcgccga
      241 cggcggcttc gtttcgctca atcatccgaa accattcgga ccgccctggg aatatcccga
      301 gctgaccggc ttcgatgcga tcgaaccgtg gaacggctgg tggaaccgcc tcaacgacgt
      361 ctcgacgcgc tactggcagg agcgactcac cacaggcgaa cgcgtctgcg gtctcggcgg
      421 cagcgacatg cactacctcg ggatctcggg tgatgcggac aacccgctct ccccggccca
      481 gatgggaatg ccgacgctct ggatccagac cgacagtccg ctcagcgcaa catcgatcct
      541 cgaagccctc cgcgccggcc gcagtttcat ctcggaatcg ccgaccgggc cgcagatctt
      601 cgtccagtac gacgcggatc ttctgcggtt agcgatcgac atcgtcggcg ccgcggggga
      661 cgcgctggtg gtcgtcgccg atgcggggat cgtctttgcc ggagcgatcg cgagcgattc
      721 ggatgccttc agctgggacg gagacgtcct cgcatcactc ttcaacggat cacgattcct
      781 ccgcgccgag attcatcgcc tcgggggcgg catccgcgcc ctctcgcagc ctatctggct
      841 atgaatcgtg tttggagagc gctcggagca cggccaactg atcttcaatc cgtcgactta
      901 gcacctttgc ttcacttcgg agttgctgaa tcttccgtct ggaaataacg tcgtcgtagt
      961 ccgcggcatt cctgatcttt cgaagtcgcg cgagcgaacg aaacaggagg ggatccacga
     1021 acgagacact tgtcgccaca tccatgtgtt cagaacttcg acccgagcgg acgtatccga
     1081 gatcctgttc acagtatgcc cgcgtttccc agaataccgc gtagtagagt cttccaatct
     1141 gcgtccgggc taccgcttcg tcagtggcat ggatgtcgag aatcgcgact gctctgagaa
     1201 agtcactccc gcgcatcttc gtccacaggg ccgccatagg ctggaaatac gccaacgagt
     1261 gattcgtcaa ctcgccccgc gtctgcgtca agccacttat agaatgagcc aaaatcgtcc
     1321 atatacgcat cgatagacac cgatggaatc gtgatcgtca gcaccagggg tggatcccag
     1381 tcgtcgtact gcttcgcctc cagaacggcg gtcgcaccgg gataagtccg ctcggcatgc
     1441 tcggtcacag cggcaatctg cgccgcgagc gtaggatgcg cgcgaagcac ggccatcacg
     1501 gattcgtaac ctatttcgct ccgattacct ggaatcaatt cctgcaccgc gaccattgct
     1561 gcctcccgaa tgcgcccgat gatttcttgc cccgcctcga tcatagcagg agattgacgc
     1621 cgcagtcgga cggcgttacg cgtcgtagtc ctcgtcgtcg gtctggacct gcaggatcca
     1681 attggcgcgc caggggttgt ccccgggaat ctgtggaccg ccgaactcac ccatctcccg
     1741 ctcgtcgcgc tcggcgaact cgatcattcg ctccaggaac gactcgaccg tcacctccgg
     1801 atcgtgaaaa cggaagcggc caagttcgtt gagcagcgtc gccccgggat cgaagacctc
     1861 gccatggaac gcaacctcgc gaagtcgacg cagccgttcg tcgtccgggg cgagcgtgcg
     1921 gacgagcgcc gcgagctcgt cgagcccttt cgcggaccga cggctgcgca tcgcggcatc
     1981 gggcccgaga ccgagcatgt cctgctgctc gcgatctcgt cgccactggg ctattgcctc
     2041 gattcgttcc gcaatcgcga ttcgcgcgtc gctcatctct cgtcgcccct ctgcatatgc
     2101 cgcacggttc gatcgcgcga cgcaatttcc tccacgtatt atggatgctt tgggagcaag
     2161 cgccgacaga tcgggatgcc gggacgaatg gaaggcgaat cacgggtcca atcgaatact
     2221 cccggaatcg cgatcgagac gtcacgcccg gtcgatgcct atctctccct ggccctcgcg
     2281 atggcgatcg tcggcagctc ggtcgtcacc ggcaagtaca tcaccgatgg catgccgatc
     2341 ttcctcgccc tcctgctccg cttcgccgga gcggtcgtcg tgatggtcgc ctggctcgga
     2401 cccgcagggc ggatccgagc ccacctcatc ctctcgcgtc gcgaccacac cgtcatcgcg
     2461 atccaggcgc tcgcgggatc ggtcgccttc aatgtactcc tgctctacgg ccttcgggag
     2521 acgaccgccg cggcgagcgg agtcatcacg agcgcaactc cagtgatgat cgcgatcatc
     2581 tcgttcctgc tcggcgaacg ccttcgcggt cgagtctggg ccggggtact cttgacgatg
     2641 gccggcgttg cgaccgtgaa tgtcttcggc gtggatggcg gcacggatca cgccgcgaac
     2701 gccgtgcttg gcgcgatcct cgtcctgggc gcagtcgtcg gcgagagcct ctatacaatc
     2761 ctcgcgaaga tggtcagccc gggcgtttcg ccaacgacga tggccctctg gtcgatcatc
     2821 tattcgacga tgatgttcct gccgctcggc ctctggcaga tgcgcggatt cgacccgtcc
     2881 gccgtaccaa cgagcgcgtg gctcagcgtc gcgtattccg cggtgatcgt gacggttgcc
     2941 gccttcgtgc tctggtttcg gggtttgaag caggttccgg cgaccacagc ggccccattc
     3001 acgggcgtga tcccgatcgt ggccgtcatc ctcgccgcga ttctccttgg cgagccgatc
     3061 gggtggccac acctcgttgg catcgtctgc gtgatcggcg gcatcctgct catcacccga
     3121 actccgtcgt cacaggagat cgcgtgaccg ctatccgaac gaggcgaatc cctcgaccgc
     3181 caatgtatcg agcatccgct gcacccgctt tccggtgacg ggcaacacca tgtcgtcgct
     3241 gaaaacctca tggttcgatt ccagccatcc tccatctccg agcttctcgg cagcatcccg
     3301 gatttccgcc tgggggattc tcaccgggtg cacgcaccag gccagaaagt ccagcaacgg
     3361 ccgctccaga cgcgacgccg agcccgaaaa cttcggcagc accttcatca gcaccgcacg
     3421 atcgaatacg gcctcgatcg tcggatcaga aaagttatgc ataccattgc caacgaacgc
     3481 ggcgacctca tcgaatgtcc gatagccaaa attcatccgg cttctcgcca ggagttgatt
     3541 gagggtctgc agccaccccc ggatctgcgg atattccgat agcacgagtc cgatctcggc
     3601 cttgtcgatg cggatgaatt caccgccgcg ggagaagtcg accagcagcg catcacgcgc
     3661 ggcctccgtc atcgcggcgt cgacggatgt cgctatcggc ggatagtcag tgaagtcgac
     3721 gtccgacagc tccatggtga acgcacggtc gaggaccttc gggctgaaga tatgcgtcgt
     3781 ctcgtccatg ttgaccgtgc cgatgatata gagattgggc ggaagtcgaa tctcggacgg
     3841 cggaacctcg aagtcgtccg tgctttcgtc gggctcgggt cgctcgatcc ggatcgcttc
     3901 tcgtgtccat ccatccgcat cgcgaccgga ttcgaggaca ctgagaagat ccgcgaagta
     3961 atactcgacg tgggcgaggt tcattt
//
LOCUS       DASTMD010000810         1813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2577420, whole genome shotgun sequence.
ACCESSION   DASTMD010000810 DASTMD010000000
VERSION     DASTMD010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577420"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VFQ54_12025"
     CDS             complement(<1..1056)
                     /locus_tag="VFQ54_12025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165763.1"
                     /translation="MATTPTPTLTPEPVGIRSVTKLQWKMLGASWIGYFLDGFDFVII
                     TLVLSDVSTQFHLNLVQAASLVSAAFVPRWLGGLLLGAIGDRWGRKPAMILSILCYSL
                     GTLLCGLSWDYWSLFAFRALVGLGMAGEYGAGSTYVMESWPKRMRNAATGIWLSSYPI
                     GTMLTALLFRLVVPTWGWRWMFIIGIVPVVAALVIRRTMPEATEWTLRVGAAKVITTV
                     ELLFSPKRRVVNIVTCLVILACLVLIFSNSSGAFTLPLGLVAAAGFVSFIVQLVGKLW
                     PMMIALVFTIFFLYISNYPIASLLPTYLKGVGYDPGQIATAVTWSALGWVVGSITWGF
                     IADTRLGTRGTYVIAMIL"
     gene            1371..>1813
                     /locus_tag="VFQ54_12030"
     CDS             1371..>1813
                     /locus_tag="VFQ54_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU0165764.1"
                     /translation="MGNYETQLYAKDGAVARITFNRPERRNATDVKFYEDFIACLEEA
                     EADPEVRSIVLAGAGTVFSAGQDLKWSATCTEADFDEYNRLINIAWNKIRKLSKPVIA
                     RVHGDALGGGMYMSTRCDLIAMKSTARMAMREIVTGEQSGGTHLLT"
BASE COUNT          374 a          539 c          598 g          302 t
ORIGIN      
        1 caggatcatc gcgatcacat aggttcccct ggtgccgagc cgggtgtcgg cgatgaatcc
       61 ccaggtgatc gatccgacga cccagccgag cgcggaccag gtgacggcgg tcgcgatctg
      121 gcccgggtcg tatccgactc ccttcaggta ggtgggcagg agggatgcga tcgggtagtt
      181 gctgatgtac aggaagaaga tcgtgaacac gagggcgatc atcatcggcc agagcttgcc
      241 gaccagttgg acgatgaagc tcacgaaccc cgcggcggcc accaggccca gcgggagggt
      301 gaacgcaccg gagctgttgc tgaagatcag cacgaggcag gccaggatca cgaggcaggt
      361 cacgatgttg acgacccgcc gcttcgggga gaagagcaac tcgacggtcg tgatcacctt
      421 ggccgcaccg acgcggagcg tccactcggt cgcctccggc atcgtgcggc ggatgacgag
      481 tgcggccacg acgggcacga tcccgatgat gaacatccag cgccatcccc aggtcggcac
      541 gaccaaccgg aagaggaggg cggtgagcat cgttccgatc gggtaggagc tcagccagat
      601 cccggtcgcg gcgttgcgca tccgcttggg ccacgactcc atcacgtagg tcgagcctgc
      661 gccgtactca ccggccatgc cgagtccgac cagcgcgcgg aacgcgaaca gcgaccagta
      721 gtcccacgag agcccgcaga gcagcgtgcc gagcgagtag cagaggatgc tcaggatcat
      781 cgcgggtttg cgcccccatc gatctccgat cgctccgagc agcagaccgc cgagccatcg
      841 cggaacgaag gccgcgctga ccaggctcgc cgcctgcacc aggttcaggt ggaactgagt
      901 cgacacgtcc gacaggacga gggtgatgat gacgaagtcg aaaccgtcga ggaagtaccc
      961 gatccacgat gccccgagca tcttccattg cagcttggtc acacttcgta ttccgaccgg
     1021 ctccggggtg agggtcgggg ttggcgtcgt tgccattaat gcctccgttg gatccactat
     1081 gactttcaag gagagtgggc agcaggtttc tgcgacccat cgagatttcc atatgcttct
     1141 gctcggatgt ctccccggtc aacatcgatg cacaaacgtg cgcccggtcg gcccgtttcg
     1201 tgtggtggat ggcttcggac ggcatcggga gggtgatgag cggacgcacg tgcacgcctc
     1261 tcaccgacag cgtgcagaca gtgtgcacag atttccattt cattgcctgg gcgaaaatct
     1321 ccgtgtgata gtgaagccga ttcgatcgca acgatgtgtg aggaaatgac atgggaaact
     1381 acgagacgca gctctacgcc aaggacggcg cggtggctcg catcacattc aacaggccag
     1441 agcgtcgcaa cgcgacagac gtgaagttct atgaggattt catcgcgtgc ctcgaggagg
     1501 ccgaggcgga tcccgaggtt cgctcgatcg tgctcgccgg agcgggaacg gtgttcagcg
     1561 ccggccagga cctcaaatgg agcgcgacgt gcaccgaggc ggacttcgat gagtacaacc
     1621 gcctgatcaa catcgcgtgg aacaagatcc gcaagctgtc gaagcccgtc atcgcgcgtg
     1681 ttcacggcga cgcgctcggt ggcggaatgt acatgtcgac gcggtgcgac ctcatcgcca
     1741 tgaagtcgac tgctcggatg gcgatgcggg agatcgtgac cggtgagcag agcggcggaa
     1801 cgcacctgct gac
//
LOCUS       DASTMD010000811         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_523931, whole genome shotgun sequence.
ACCESSION   DASTMD010000811 DASTMD010000000
VERSION     DASTMD010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523931"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /gene="purH"
                     /locus_tag="VFQ54_12035"
     CDS             <1..1389
                     /gene="purH"
                     /locus_tag="VFQ54_12035"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU0165765.1"
                     /translation="PEILDGRVKTLHPKIHGGLLARRDLPDHVRQLEEHEITPIDILV
                     SNLYPFEETVSAPGATDETIVENIDIGGPAMVRAAAKNFATVTVVTDPGDYESVLTAL
                     KGGEAALDFRRSLAAKAYAHVSTYDSLVSEFLRTAPDSVAAFPEELSIGLRLAKTPKY
                     GENPQQRAAAYRRLRAGAPESGLLDAEFISGDDLSFNNYLDADAAWQAAQLFTEPAVA
                     IVKHMVPCGLATRAELVDAYAAALAGDPVSAYGGIVALNRTVDLATAQQLRRIKLDII
                     IAPGYDDDAREVLLRKKGTRILSLANRAAGTLRTNAFDLRPIGGGLLVQTPDIYPDDE
                     STWKVVTEVQPTDGQRQDLAFAWQAARMVKSNAIVFARDLAIVGLGAGQPNRLESVAI
                     AARKAGEAANGAAMASDAYFPFADGIEQGIAAGVTAVIQPGGSIRDEDVIAAANRAGI
                     TMIFTGTRHFRH"
     gene            complement(1547..>2378)
                     /locus_tag="VFQ54_12040"
     CDS             complement(1547..>2378)
                     /locus_tag="VFQ54_12040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEU0165766.1"
                     /translation="NLHRGNKNAVARLWNGVNQSVLSYEVGVLLGYMARRVLGQYDLA
                     LLGREPVSSGKLYYVEPNIRGIEGKLGLPREDFRMWLALHETTHAFEFEAHPWVREHF
                     NSMLDRYMGLMKHDADQLKQGLQGLRTIAGCARSQDGRETGSWIEAFMSPEQRALFNE
                     MQAMMCVIEGYSNHIMNAVGKTLLENYELISHRFEQRQAQRSQSDQLFARLTGLDVKM
                     EQYRAGEQFIDSIVDERGHTFAHRVWDGPENLPTMDEIRNPDKWIRRVDMRVLAPVTS
                     "
BASE COUNT          435 a          777 c          744 g          422 t
ORIGIN      
        1 cccgagatcc tcgatggacg cgtgaagacg cttcacccga agatccacgg cggcctgctc
       61 gcacgccgcg accttcccga tcacgtccgg caactcgagg agcacgagat cacgccgatc
      121 gatatcctcg tctcgaatct ctaccccttc gaggagaccg tctccgcccc cggcgcaaca
      181 gacgaaacga tcgtcgagaa catcgacatc ggtggcccgg cgatggtgcg ggcggcggcg
      241 aagaacttcg caacggtcac tgtcgtgact gatcccggcg actacgagtc ggtcctcacg
      301 gccctcaagg gcggggaagc cgcgctcgac ttccggcgct cgctggcggc gaaggcatac
      361 gcccacgtct cgacgtatga ctcgctggtt tcggagttcc tccggacagc tccggattcg
      421 gtagcggcat ttccggagga actgtcgatc gggctccggc ttgcgaagac gccaaaatat
      481 ggagagaacc cgcagcagcg cgcggccgcc tatcggcggc tccgggcggg ggcgccagaa
      541 tcgggattgc tcgatgccga gtttatctct ggtgacgatc tgtcgttcaa caactacctg
      601 gatgccgacg cggcctggca ggcggcacag ctcttcaccg aacccgcggt ggcgatcgtc
      661 aagcacatgg tcccgtgtgg cctcgcgacg cgggccgaac tcgtcgacgc ttacgccgcc
      721 gccttggccg gagatccggt ttcggcttac ggcggaatcg tcgcactcaa ccgaacggtc
      781 gatctcgcga ccgcccagca gctgcgccgg atcaaactgg acatcatcat cgcgcccggg
      841 tatgacgacg acgcgcgaga agtgctcctc cgcaagaagg gaacgcgaat cctgtcgctt
      901 gcaaatcgag ccgcgggaac cctccgcacc aacgccttcg atctccgccc gatcggcggc
      961 ggcctcctcg tccagactcc ggacatctat cccgatgatg aatcaacatg gaaggtcgtg
     1021 accgaggtcc agccgactga cgggcagcga caggatctcg ccttcgcctg gcaggcagcc
     1081 cggatggtca agtccaacgc gatcgtcttc gcgagggatc tggcgatcgt cggcctcggc
     1141 gcaggtcaac cgaaccggct cgagagcgtc gccattgccg ccaggaaggc cggagaggcc
     1201 gcgaacggcg ccgcgatggc cagcgatgcc tacttcccgt ttgccgacgg tatcgagcag
     1261 ggaatcgccg ccggcgtcac ggccgtcatc cagccgggcg gatcgatccg cgacgaggac
     1321 gtgatcgcag ccgcgaacag ggccgggatc acgatgatct tcacgggtac ccgccacttc
     1381 cgccactagg cggcgattga tcccgtcgcc acgggggcac gatccccggt cgttcgtagg
     1441 gcggcatgtg gcaatcgtct cggccgtcag tgtggacgca gaagagccgc cggagttctt
     1501 tccggcggct cttctgcgtc tggttcgcgt gtcgtcggga aaccgtctag gacgtgaccg
     1561 gggcaagcac cctcatgtcg acgcggcgaa tccacttgtc cggatttcgg atctcatcca
     1621 tcgtcggcag attctccggg ccatcccaga cgcgatgcgc gaaggtatgc ccgcgctcat
     1681 cgacgatcga atcgatgaac tgctcgccgg cccggtactg ttccatcttt acgtcgagac
     1741 cggtcaggcg cgcgaagagc tggtcggact gagaccgctg cgcctgtcgt tgctcgaagc
     1801 ggtgcgagat gagctcgtag ttctccagga gcgtctttcc gacggcattc atgatgtggt
     1861 tcgagtatcc ctcgatcacg cacatcatcg cctgcatctc gttgaagagc gcgcgctgct
     1921 cggggctcat gaatgcctcg atccagctcc cggtttcgcg gccgtcctgg gatcgcgcgc
     1981 aaccagcgat cgtgcgcagc ccctggagac cctgcttgag ttgatcggca tcatgcttca
     2041 tcagacccat gtaccggtcg agcatcgagt tgaaatgctc gcggacccag gggtgggcct
     2101 cgaactcgaa ggcgtgggtc gtctcatgga gtgcaagcca catccggaag tcctcccgcg
     2161 ggagtccgag cttgccttcg atgccccgga tattcggctc gacgtagtag agcttgccgg
     2221 acgagaccgg ctcacgacca agcagggcaa ggtcgtactg cccaaggaca cgtcgagcca
     2281 tatagcccag cagcacgccg acctcataac tgaggacgga ctgattgacg ccgttccaga
     2341 gcctggcaac ggcattcttg tttccgcgat ggaggttg
//
LOCUS       DASTMD010000812         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1235900, whole genome shotgun sequence.
ACCESSION   DASTMD010000812 DASTMD010000000
VERSION     DASTMD010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235900"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFQ54_12045"
     CDS             <1..810
                     /locus_tag="VFQ54_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0165767.1"
                     /translation="DVGDMVKTYTRRRGFRSTTVDAVRDVSFTLEHGKISALVGQSGS
                     GKSTIGRLLTGIEKPDSGHIIFTGSGRAAADVTTFRRRQLRDYRRHIQLVFQDPYSAL
                     NPAHPIAHSLSQPLRNYRGLGRGDVRAEAMRMLEEVGLQPAERFIDKFPHQLSGGQRQ
                     RVVVARALAPKPEILIADEPTSMLDVSIRAEILELLSDLVRNQNLAMLYITHDLLSAR
                     MLADEIMVLNKGRIVEDGVTDAVIANPRDEYTQLLLRSIPNPFAPADRAAD"
     gene            911..>2037
                     /locus_tag="VFQ54_12050"
     CDS             911..>2037
                     /locus_tag="VFQ54_12050"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU0165768.1"
                     /translation="MKRRSFIAGLGSLPLAATATAAAQSTPIAGTPVPEATPIPVGSA
                     YGTVSIRVMTFNIWIGGDQVDFERVAEAIQLAKADVVGVQEAEGNIPRLARRLGWSYY
                     DTRMHIVSRFPIIDPEGANGNYVLIEPVTGGVFAMSNVHLPSDPYGPQTVIDGTTPEA
                     LMQLEIDTRLPALQPKLDACRQLIDAGFPVFLTGDYNSPSNLDWTATTGDVRGVKRFP
                     FAWPVAKATEDAGFRDSYRTLYPDPAAKVGYTWTAGYPAPLLRSGEPLDRIDFVWFAG
                     DIDVTASQIVGEAGGADVDIPVTPYPSDHRSVVSAFNVNLVTPPIYTSVARRAVEVGE
                     RQTVRFHARGDQTDTIAFMPADGTPADAPVTFIPRETWVDGS"
BASE COUNT          374 a          694 c          604 g          365 t
ORIGIN      
        1 gatgtcggcg acatggtcaa gacctacacc cggcggcgag gcttcagatc gacgaccgtc
       61 gacgcggtgc gcgacgtctc gttcaccctc gagcacggga agatcagtgc actcgtcggg
      121 cagtccggaa gtggcaagtc cacgatcggg cggttgctca ccggcatcga gaagccggac
      181 tccggacaca tcatcttcac cggcagtggc cgggcggcag cggacgtgac gacgtttcgt
      241 cgccgtcaat tgagagacta ccggcgacac atccagctcg tcttccagga cccctactcc
      301 gcgctcaacc ccgctcatcc gatcgcgcat tccctctccc agccgctccg caactatcgg
      361 gggctcggtc gcggtgatgt gcgtgcggag gcgatgcgta tgctcgagga ggttgggctc
      421 cagccggccg aacgcttcat cgacaagttc ccgcatcagc tctcgggtgg ccagcgccag
      481 cgcgtcgtcg tcgcccgcgc actggcgccg aagccggaaa tcctgatcgc ggacgagccg
      541 acttcgatgc tcgacgtctc gattcgcgcg gagatcctcg agttgctgag cgatctcgtc
      601 cgcaatcaga acctggcgat gctctacatc acgcacgatc tcctgagcgc acgaatgctg
      661 gcggacgaga tcatggtcct caacaagggc cggatcgtgg aggacggcgt caccgacgcc
      721 gtgattgcca atccccgcga cgagtacacg cagctcctgc ttcgttcgat cccgaatccg
      781 tttgcccccg cggacagagc ggcagactga accgcgcagc gtaacgccac cttgatgatg
      841 cacgcgttcc tcttctccag aatgagatct gtacgacacc attgcagatc gatcttgaga
      901 aggaactccc atgaaacgac gctcgttcat cgcgggactc ggcagcctgc ccctcgccgc
      961 cacggcgact gctgccgccc aatcgacacc aatcgctggc acgcctgttc cggaagcgac
     1021 cccgatcccc gtgggtagcg catatggcac cgtttccatc cgggtgatga cgttcaatat
     1081 ctggatcggt ggtgaccagg tcgacttcga gcgcgttgcc gaggccattc aacttgcgaa
     1141 ggctgacgtg gtcggcgtcc aggaagcgga gggcaacatc ccccggctcg cgcgtcgact
     1201 cggctggtcc tactacgaca caaggatgca catcgtctcg cggttcccga tcatcgatcc
     1261 cgagggcgca aacggcaact acgtcctgat cgagccggtg acgggcggcg tcttcgcgat
     1321 gagcaacgtc caccttccgt ccgatccata cggaccgcag accgtgatcg acggcacgac
     1381 gccggaagcg ctgatgcagc tcgagatcga tacccgcctt ccggcgctcc aaccgaagct
     1441 cgatgcctgc aggcagctca tcgacgcggg atttcccgtc ttcctcaccg gggattacaa
     1501 ctcgccgtcg aatctcgatt ggacggcgac aaccggcgac gtccgcggtg tgaagcgatt
     1561 ccccttcgca tggccggtcg ccaaggcaac cgaggatgcc ggtttcaggg attcctatcg
     1621 cacgctctat ccagatcccg ctgcgaaggt cggctacacg tggacggctg gctaccccgc
     1681 gccgctgctg cgttccggtg agccgttgga tcgcatcgac ttcgtctggt tcgccggtga
     1741 catcgacgtg accgcgagcc agatcgtcgg tgaggctggc ggagccgatg tcgacattcc
     1801 ggtcacgccg tacccgtcag atcatcgctc cgtggtttcc gccttcaacg tgaacctcgt
     1861 cacgccgccc atctacacat ccgtcgcccg gcgagccgtc gaggtcggag agcggcagac
     1921 ggttcgcttc catgcccgcg gcgatcagac cgacacgatt gcattcatgc ccgcggacgg
     1981 aacacccgcc gacgcaccgg tcacattcat ccctcgcgaa acctgggtcg acggatc
//
LOCUS       DASTMD010000813         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1950698, whole genome shotgun sequence.
ACCESSION   DASTMD010000813 DASTMD010000000
VERSION     DASTMD010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950698"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VFQ54_12055"
     CDS             complement(<1..1051)
                     /locus_tag="VFQ54_12055"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEU0165769.1"
                     /translation="MPTTLIDRSPFGELDGKTVERFTLENESGLFVALINYGAAIQEL
                     WFQDSAGTRNNLVLGFKTFAEYRGKNPHFGSVLGRYANRIRDARFPLDGEIIHVTANR
                     PPISAHGGARPFDKYVWDAEVVETGDGTGVRFSHVSPDGDEGYPGTFQVSVTYTLTAE
                     NGLKLAYEATTDKPTIHNLTNHCYFNLRGEENGVVDDQVLQVFASAFAEADPDQLMTG
                     NLISLDGNSLDFRQPKRIAEVMRDASNPQVALARGIDQCFLLDRDDPSDASLILAARL
                     EDPGSGRVMEVLTTEPGVQIYSSNSLDGSVAGFSGRLYRQSDAICFETQHLPDSANHP
                     NFPSTVLRPGETYAST"
     gene            complement(1175..2227)
                     /locus_tag="VFQ54_12060"
     CDS             complement(1175..2227)
                     /locus_tag="VFQ54_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165770.1"
                     /translation="MNPVIIHPGTAPGPDLVGLIVTQEVSNPVTGKRAFHKGHRIAAA
                     DLEVISRLDRDLHAVRIDATDVHEDVAGVRLAELIRGDGVTQRNPVQSRVNIVSERKW
                     LLRVDAEAVFTINRHPGIGVFTALDRLPVLPGKIVAGAKIAPVAIPGETLTEIAGYLA
                     SRPEPMLRVKPFIPYCVGVIVTEGLSESVRDRFEGVVQRKLRWYGANVLRFEHVPNVA
                     AAIANAADALLADGAELLLTAGGNMMDPLDASIQALPEFDASIVRLGAPAHPGSMFWL
                     GYTNEHEIPVVNLASCSMYSKSTVADLVLPWVMAGEQVTSDDLATLGYGGLLDRNMGW
                     RFPPYDEQEVDEPDEE"
     gene            2497..>3305
                     /locus_tag="VFQ54_12065"
     CDS             2497..>3305
                     /locus_tag="VFQ54_12065"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0165771.1"
                     /translation="MRITDVKLRQVTGTLPVEGEFWEERLIQPLDIYPEYRNGSRREL
                     NKTNERGVPISSCFVEIETDEGITGIGGPVSVDVAFIILRQFRSFLIGEDPRANERIW
                     DIMYRSAVHGRKGEAMFAISAVDCAIWDLRGKWANASVCQLLGGPNRTEVKAYASALG
                     FSIEPERAAAQAKKFVDEGYTATKWFVRNGPADGKEGIRKNVELVAALREAVGPDVEI
                     MIDAWMSWDVPYTVKMSQLLIDYNPRWFEEPVMPDKIDSYAQARAQSLVPIV"
BASE COUNT          590 a          999 c         1138 g          578 t
ORIGIN      
        1 tggtcgaggc atacgtctcg ccggggcgaa gcaccgtcga cgggaagttc gggtggttcg
       61 cggagtccgg gagatgctgc gtctcgaagc agatcgcgtc gctctgccgg tagagccgac
      121 cgctgaagcc ggcgaccgag ccatcgagcg agttgctgct gtagatctgc acgcccggct
      181 cggtggtgag tacctccatc acgcggccgg acccgggatc ctcgaggcgc gcggcgagga
      241 tcagcgatgc gtcactcggg tcgtcgcggt cgagcaggaa gcactggtcg atgccgcggg
      301 cgagcgcgac ctgggggttc gacgcgtctc gcatcacctc ggcgatccgc ttcggctggc
      361 ggaagtcgag cgaattgcca tcgagcgaga tcaggttgcc ggtcatcagc tggtcgggat
      421 cggcctcggc aaaggcgctt gcgaagacct gcaggacctg atcgtcgacg accccgttct
      481 cctcgccgcg cagattgaag tagcaatggt tcgtcaggtt gtggatggtc ggcttgtcgg
      541 tcgtcgcctc gtacgcgagc ttgaggccgt tctcggccgt cagcgtgtag gtcacgctga
      601 cctggaacgt gccgggatag ccctcgtcac cgtcggggct gacgtgggag aagcggacgc
      661 cggtgccgtc cccggtctcg acgacctcgg catcccagac gtacttgtcg aacggccggg
      721 cgccaccgtg ggcgctgatc ggcgggcgat tcgccgtgac gtggatgatc tcgccgtcga
      781 gcgggaaccg ggcgtcacga atccggttcg catagcgtcc gaggaccgat ccgaagtgcg
      841 ggttcttgcc gcgatactcc gcgaacgtct tgaagccgag tacgaggttg ttgcgcgttc
      901 cggcggaatc ctggaaccag agttcctgga tcgcggcgcc gtaattgatg agcgcgacga
      961 agagcccgct ctcgttctca agagtgaagc gctcgacggt cttgccgtcg agttcgccga
     1021 acggcgagcg gtcgatgagc gtggttggca cgagatctcc cctttgagca ggcgcgcatc
     1081 gctacgcgag ggagcgggag gatatcccgc tcgcggattc cggacatgac gaatccgatt
     1141 gtagagcgta gggcgagggt ttcgtaagtc tgcgctattc ctcgtcgggc tcatcgactt
     1201 cctgctcatc gtaaggtggg aagcgccagc ccatgttgcg atcgaggaga ccgccgtacc
     1261 ccagcgtggc gagatcgtcc gacgtaacct gttcgcctgc catcacccac ggcaggacga
     1321 gatcggcgac ggtcgacttc gagtacatcg agcaggaggc gaggttcacg accggaatct
     1381 cgtgctcgtt cgtgtagccg agccagaaca tcgaccccgg atgcgcgggc gcgccgaggc
     1441 ggacgatcga agcatcgaat tccggcagcg cctggatcga ggcatcgagc ggatccatca
     1501 tgttgccgcc cgccgttagc agcagctcgg cgccgtcggc cagcagggcg tcggcggcgt
     1561 tcgcgatcgc cgcggcgacg ttcgggacgt gctcgaatcg cagcacgttc gcgccgtacc
     1621 agcggagctt gcgctggacg acgccctcga agcgatcgcg aacggattcg ctgagcccct
     1681 cggtcacgat cacgccgacg caatagggaa tgaacggctt cacccggagc atcggctccg
     1741 gacgactcgc gaggtacccg gcgatttctg tcaacgtctc gccggggatt gcgacgggcg
     1801 cgatcttggc gccggcgacg atcttcccgg gcagcaccgg gagccggtcg agcgcggtga
     1861 agacgccgat tcccggatgg cgattgatcg tgaagaccgc ctcggcatcg acgcgaagga
     1921 gccacttccg ttcggagacg atattgacgc ggctctggac cggattccgc tgggtgacgc
     1981 cgtcaccacg aatcagttcg gcgaggcgga ccccggcgac gtcctcatga acgtcagtcg
     2041 cgtcgatccg gacggcgtgg agatcgcggt cgagccggga gatcacttcg agatcggcgg
     2101 cggcgatgcg atgtcccttg tggaacgcgc gcttgccggt gacgggattg gagacttcct
     2161 gggtgacgat gagtccgacg agatcggggc ccggtgcggt gccaggatgg atgatgacgg
     2221 ggttcattca gatagggact ccaattaggg gcgtcgggaa agtaggtgac aatcgggtcg
     2281 tcaaacgctg agtatcatct tggcactgac ggacggtaag cgcaatgaga atgagtcctt
     2341 gttgccgcag gaatggcgcg tcacccgtcc cgcggatcac tcttcccaca tcgggatcgc
     2401 cttggtatcg tgtcgccact cgacatccga agaccgttcg atggcacgcc aacgacaccc
     2461 tcagggccaa cggcgtccgc tccagggaga tggatcatgc gcattacgga cgtgaaactg
     2521 cgacaagtca ccggcacact tccggtcgaa ggcgaattct gggaagagcg tctcatccag
     2581 ccgctcgaca tctacccgga gtatcgcaac ggctcgcgcc gggagttgaa caagacgaac
     2641 gagcgcgggg tgccgatctc ctcctgcttc gtcgagatcg agacggatga ggggatcacc
     2701 ggcatcggcg gaccggtctc ggtggacgtc gccttcatca tcctgcgcca gttccgcagc
     2761 ttcctcatcg gtgaggatcc gcgggcgaac gagcggatct gggacatcat gtaccgctcg
     2821 gccgtccacg ggcggaaggg cgaggcgatg ttcgcgatca gcgcggtcga ctgcgcgatc
     2881 tgggatcttc gcggcaagtg ggcgaacgcg tcggtctgcc agctgctcgg cggcccgaac
     2941 cgcaccgagg tcaaggcgta tgcgagcgcc ctcgggttct cgatcgagcc cgaacgcgcc
     3001 gccgcgcagg cgaagaagtt cgtcgacgag ggctacaccg cgacgaagtg gttcgtccgc
     3061 aacggcccgg ccgatggcaa ggaaggcatc cgcaagaatg tcgaactggt cgctgccctg
     3121 cgtgaggcag tcggacccga tgtcgagatc atgatcgatg cctggatgag ctgggacgtc
     3181 ccctacacgg tgaagatgtc ccagctcctc atcgattaca acccgcgctg gttcgaggag
     3241 ccggtgatgc cggacaagat cgactcctat gcccaggcac gcgcgcagtc gctggtgccg
     3301 atcgt
//
LOCUS       DASTMD010000814         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2486971, whole genome shotgun sequence.
ACCESSION   DASTMD010000814 DASTMD010000000
VERSION     DASTMD010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486971"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFQ54_12070"
     CDS             <1..258
                     /locus_tag="VFQ54_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165772.1"
                     /translation="LAPLPVPANHVVASPTSTQNASRHPVTATHVVASLTSAQNASHY
                     SRPHGACRHFGLAESAIYANGSGTFRRSDLHRLRPHAPRPV"
     gene            613..1533
                     /gene="glp"
                     /locus_tag="VFQ54_12075"
                     /pseudo
     CDS             613..1533
                     /gene="glp"
                     /locus_tag="VFQ54_12075"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
     gene            1549..1824
                     /locus_tag="VFQ54_12080"
     CDS             1549..1824
                     /locus_tag="VFQ54_12080"
                     /inference="COORDINATES: protein motif:HMM:NF015419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165773.1"
                     /translation="MIGVPADQIDRPKPRVILTEDVEPSDRIEYQRGIVRVDAEGRLV
                     GTNTGPQRSSRLQSFLGYNALLVIAPRESVYKAGERVDALMIAPPLA"
BASE COUNT          381 a          738 c          597 g          336 t
ORIGIN      
        1 ctcgcaccac tccccgtccc ggcgaaccac gtcgtcgcct ctccaacgtc gacacagaac
       61 gcctcgcgcc accccgtcac ggcgacccac gtggtcgcct ctctaacgtc ggcacagaac
      121 gcctcgcact actcccgtcc ccacggcgct tgtcgccatt tcggactggc agagagcgcg
      181 atttatgcca acgggagcgg cacatttcgc cggtcagacc tgcatcgcct ccgcccacac
      241 gcaccccgtc cggtctgacg gctgcagcgc cgccctacgc acccccgcgt ttcaaacgat
      301 ccatctttgc actgctcgct gctctcgcag aacgaccatc gaaacagcgt tcgtgggtag
      361 ggtgggactg cggagtccaa gactggcgac caagacgtcg ttcgatcacg atgcccggtc
      421 tttggacgca gcaacgtctc gctcccctcc tccagccaga cgccatccac ctgcccgcga
      481 ccacccccga cctacgaaaa caccggccat gctaggatgc ccgcagattt ggtcaatcgc
      541 cgacatcgca tgaaccatcg aggttttccc gtgacgcaat ctccacccat cgatttccag
      601 gaactcgacc gcatgcgccc gcccgacgag gcccgcgcca tcatctcccg cttcatcacg
      661 acgcttcccg gcgaactcat cccgctgaag gacgcggccg gtcgtgttct cgccgaagac
      721 atcgtcgcca gggaagatca tccccccttc cctgcctcga cgatggacgg ctacgccgtg
      781 ctcgccgaag acggctcgcc gtggcgcgag attctcgccg tccagaacgc gggaaatgtc
      841 gtcgattccg aggtcacgga aggctatacc gtccgcatca tgaccggcgc cccgatcccg
      901 cagggcgcga acgccgtcgt ccagatcgag gcgacctagc tggcggatga ccacgtcatc
      961 atccatcagg aggacgtgac gccgggtgag aacatccgcg ccgtcggctc cgatgtccgc
     1021 aagggcgccc tgatcctcgc gaagggcacg cggatcggcc ccgccgagat cggcctcatc
     1081 gcgagcatgg gcttcgatcc ggtcgcggtg acccgcaagc cacggatcag cgtgctctcg
     1141 accggcgatg aactcgtcga gccggatcag ccgttggcgc cgggccagat ccgggatgca
     1201 aaccggttca gcctgatcgc cgccctcacc caggaacctg tcgaaatcac ctggttcggg
     1261 cgcgccccgg acaagcgtga cgaactcgag ggccttctcc gcgatcgcct cgatcaggac
     1321 gacatcatca tcacctcggg cggcgtctcg atgggcgatc tcgatctgat caaggcgatc
     1381 ctcttcgacg ccgaggatgt cgacgttcat ttccgccgtc tttatatgaa gccggggaag
     1441 ccgctcaatt tcgcgacgca cggccagacg ctgatcttcg gtctgcccgg caacccggtc
     1501 tcctcgctcg tctgcaccga gctcttcgtg cggaccgcga tccggcagat gatcggcgtc
     1561 ccggcggatc agatcgatcg tcccaagccg agggtgatcc tcaccgaaga cgtcgagccg
     1621 tcggatcgca tcgagtatca gcgtggcatc gtccgcgtcg atgcggaggg gcggctggtc
     1681 gggacgaaca ccggaccgca gagatcgtcg cggctgcaat cgttcctcgg ttacaacgcg
     1741 ctcctcgtga tcgcaccgcg cgagtcggtg tacaaggctg gcgaacgggt cgacgccctg
     1801 atgatcgcgc caccactcgc gtagcgcgat ccccgatcgc acgcgaatct cccatccgtc
     1861 acgaaggaaa ccgcatggca aagtcgtcga aagacaagga caggaagtca aagaaggttg
     1921 cgggcgcgac gggcgtcgcc gagttgccag gactggcgga acaccaggcg aggatcgccc
     1981 cgaactggtc gctctcgtcg gcggctgccg cgatcgatcc gacggcatac ccggtccgca
     2041 gcggacgcgc aa
//
LOCUS       DASTMD010000815         4314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_344678, whole genome shotgun sequence.
ACCESSION   DASTMD010000815 DASTMD010000000
VERSION     DASTMD010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344678"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFQ54_12085"
     CDS             complement(<1..239)
                     /locus_tag="VFQ54_12085"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEU0165774.1"
                     /translation="MAHPLLELLESRVVLGDGAMGTMLYEAGRSLDECLDAINLSQPD
                     IVARIHQNYLSAGADVIETNTFGANRYRLEQHGLA"
     gene            564..1427
                     /locus_tag="VFQ54_12090"
     CDS             564..1427
                     /locus_tag="VFQ54_12090"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEU0165775.1"
                     /translation="MSLEGTGIITDTQPIMTTSNESTVQARGVLMPIGGAEDKLDDKI
                     ILSTFAQLAGGKTARIAIVPTASSIETAGLRYKAIFLGMGVEDAEVVYIGSREDANSP
                     EVLEMVEQATGIFLTGGNQLRLSAIIGGTRFEELVKERNQQGVVVAGTSAGASILSDH
                     MVALGASGNTPKLRMAQMMAGFGLIRDVILDQHFRQRDRIGRLLALVASNPRLLGVGI
                     DEDTAAMIDDQGVIEVIGRRSITIVDGSEMFSDIFQVKAYGEITVSNAKLHVLTPGRR
                     FDMRTRSLILG"
     gene            1568..4204
                     /gene="cphA"
                     /locus_tag="VFQ54_12095"
     CDS             1568..4204
                     /gene="cphA"
                     /locus_tag="VFQ54_12095"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEU0165776.1"
                     /translation="MLEILETTIYRGANVWARMPAIRYKVDLGELETRPSNKIPGFYE
                     HLTELIPSLYDHGCSIGKPGGFLQRLREGTWMGHVMEHVALELQNLAGAEVVRGKTRS
                     TTEKGVYNVVFQYDQEDVGLAAGELSLRLLNHLVYNSEPDFDFVSELETKVIKLAERL
                     AYGPSTGAIVQEAVRRGIPVIRLDPQRSLVQLGHGRFQRRIWATVTSETANIAVEVAS
                     NKELTNRLLHEVGIPVPQSRVVRTEDAAVSAAGRIGYPVVLKPLDGNHGRGVCINLAD
                     ASEVREFYSIAERESRNGAVIVESYVTGKDYRILVVDNQVVAVAERVPAHVVGDGEHT
                     VQDLIDITNSDPRRGVGHEKILTRIHVDSQTNEVLERQGITLEDIPGPGQFVQLKLTG
                     NMSTGGTSIDRTDDIHPDNVEIARQAAMVVGLDIAGIDFIAEDISRSVRQASGAIVEV
                     NAGPGFRMHTHPTEGHPRHVGRAVVDMLFKRGEPSRVPIVAVTGTNGKTTTSRMIAHI
                     MKTAGRRAGLTTTDGIYIDGTQIMAGDMSGPGSARMVLKNPTIDFAVLETARGGILRS
                     GLGYDRCNIAVVTNVTSDHLGLRGVDTLAELARVKAVVPASVLPDGACVLNADNEWTV
                     GMTRVARGEIIFFSMDEESTVIQEHLRERGRAVVLKKTPRGEMITLLEHRRETSILLA
                     SQIPATFEGRARVNIQNAMAATAAAVADDIQLDHIRQALRTFTSNFSQTPGRFNLLDI
                     AGQRVLIDYCHNVAGLEAIADFVERMAADHTVAVISMPGDRSDGDIEAFGALAAKSFD
                     QLIIREDDNTRGRPSGQISQMLYDAAVRGGIDPTNVKIIVNELEACRVAVEEADKGDL
                     VVLMADKPVKVWEMLTNSGEPV"
BASE COUNT          825 a         1342 c         1389 g          758 t
ORIGIN      
        1 tccgcgagac catgctgttc gaggcggtaa cggttcgctc cgaacgtatt tgtctcgatg
       61 acatcggcgc cagcacttag atagttctgg tgaatccggg ctacaatgtc aggctgtgag
      121 aggttgatgg cgtcgaggca ctcatcgagc gagcgtcccg cctcgtagag catcgttccc
      181 attgccccgt cgcccaacac cacgcgagat tccaggagtt caagaagggg atgtgccatg
      241 gtacctctct ggttggtgca tggtcccgct tgcggggaga ccgggtgata acgcgactgc
      301 gacttgtgat tgaagcacaa gctacaccgg ccacgcgtcg ttcgacgcga aatggttctg
      361 ccaatcttaa tacagtcggg cgacaatgat ggcttgtcca tcatgcccgc cacctcccgg
      421 cgcggttgtc aggagcgtgt ttctcgccta tacgtagtcc catgcttcga cgacttgcgg
      481 atccaggttc ccgctccagg attcttcccg atcatcgtca agtggcgaca acagtccacg
      541 gaaagcgaag aggatcgatc gctatgtccc tcgaaggaac gggaatcatc actgacaccc
      601 aaccaatcat gactacatcc aatgaatcca ccgtccaggc tcgcggcgtc ctgatgccaa
      661 tcggcggcgc cgaggacaag ctggacgaca agatcatcct ctcgaccttc gcccagcttg
      721 ctggcggcaa gacggcgcgt atcgcgatcg tgccgacggc atcctcgatc gaaacggccg
      781 gacttcgcta caaggcgatc ttcctcggca tgggcgtcga ggacgccgaa gtcgtctata
      841 tcggaagccg cgaggatgcg aacagcccgg aggtgctcga aatggtcgag caggcaaccg
      901 gcatcttcct caccggtggt aaccagctcc ggctctcggc aatcatcggc gggaccaggt
      961 tcgaggaact cgtcaaggag cgcaaccagc agggtgtcgt cgttgccggg acctcggccg
     1021 gcgcctcgat tctctccgac cacatggtcg cgcttggagc gagtggcaac acgccgaaac
     1081 tccgcatggc gcagatgatg gccggcttcg gcctgattcg cgacgtcatc ctcgaccagc
     1141 atttccgcca gcgggatcgg atcggccggc tgctcgccct cgtcgcctcg aacccccggc
     1201 tgctgggcgt cgggatcgac gaggataccg ccgcgatgat cgacgatcag ggcgtgatcg
     1261 aggtgatcgg tcgccggagc atcacgatcg ttgacggttc cgagatgttc agtgatatct
     1321 ttcaggtaaa ggcctacggc gagatcaccg tcagcaatgc gaagctgcat gtgctgacgc
     1381 cggggcgccg cttcgacatg cggacccgat cgctcattct cggctgacgt gtcatccggg
     1441 aatgggtcgc ctctacagcc tcgtcagcga gatgacgagg ctgtttgcgt aggtaacaac
     1501 caggctgctg aaacggtcat gtggccggtt tccggggatg atgcaggcga cttcgggagg
     1561 tgcatgggtg ttggagattc ttgagacgac gatctatcga ggtgcgaatg tctgggcccg
     1621 gatgccggcc atccgctata aggtcgacct cggtgagctc gagacgcgcc cttcgaacaa
     1681 gattccggga ttctacgagc atctgaccga gctcatcccg tcgctctacg accatgggtg
     1741 ctcgatcggc aagcccggtg gcttcctcca gcgacttcgc gaggggacgt ggatgggcca
     1801 cgtgatggaa cacgtcgccc tcgaactcca gaacctggcc ggggccgagg tcgttcgcgg
     1861 caagacccgg agcacgaccg agaagggcgt ctacaacgtc gtctttcaat acgatcagga
     1921 agatgtcggg ctcgccgcgg gtgagctctc gcttcgtctc ctcaaccacc tcgtctacaa
     1981 ctccgaaccc gacttcgact tcgtcagtga gctcgagacc aaggtcatca agctcgcgga
     2041 gcgactcgcg tacggtccgt cgaccggggc gatcgtgcag gaggcggtgc gtcgcgggat
     2101 tccggtgatc cgtctcgatc cccagcgttc gctcgtccag ctcggtcacg gccggttcca
     2161 gcgaaggatc tgggcgaccg tcacctccga gaccgcgaac atcgccgtcg aggtcgccag
     2221 caacaaggaa ttgacgaacc gcctgcttca cgaggtcggc atcccggtgc cgcagtcacg
     2281 cgtcgtccga accgaggacg ccgcggtgag cgccgccggc cggatcgggt atccggtcgt
     2341 gctgaagccg ctcgatggca atcacggtcg aggcgtctgc atcaatctcg ccgatgcgtc
     2401 cgaagtgcgg gagttctatt cgatcgccga gcgcgagagt cgcaacgggg cggtcatcgt
     2461 cgagagctac gtgaccggca aggactaccg gatcctcgtt gtcgacaacc aggtcgtggc
     2521 ggtggcggag cgtgtcccgg cgcacgtcgt cggcgatggc gagcacacgg tccaggacct
     2581 gatcgacatc acgaactcgg atccccggcg aggcgtcggg cacgagaaga tcctcacccg
     2641 catccacgtc gattcacaga cgaacgaggt gctcgaacgg cagggcatca ccctcgagga
     2701 cattcccgga cccgggcagt tcgtccagct gaagctcacc ggcaacatga gcaccggtgg
     2761 cacctcgatc gatcgcacgg acgacatcca tcccgacaac gtcgagatcg cgcgtcaggc
     2821 ggcgatggtg gtgggactgg acatcgccgg tatcgacttc atcgccgagg acatctcgcg
     2881 ctccgtgcgc caggcgagcg gcgcgatcgt cgaagtcaat gccggaccgg gcttccgcat
     2941 gcacacgcat ccgacggaag gtcatccgcg tcacgtcggg cgtgccgtcg tcgacatgct
     3001 cttcaagcgc ggcgagccgt cgcgcgtgcc gatcgtcgcc gtcaccggca cgaacgggaa
     3061 gaccacgaca tcgcggatga tcgcccacat catgaagacg gccgggcgtc gcgccggact
     3121 gacgacgacg gacggcatct acatcgacgg cacgcagatc atggccggtg acatgagcgg
     3181 accgggcagc gcccggatgg tgctcaagaa cccgacgatc gacttcgcgg tgctggagac
     3241 ggcgcggggc ggcattctgc gctccggtct cggatacgat cgttgcaata tcgccgtcgt
     3301 cacgaacgtc acgagcgatc accttggact gcgcggcgtc gacacgctgg cggaactggc
     3361 gcgggtgaag gcggtggttc cggcgagcgt gctgccggac ggagcctgcg tgctcaatgc
     3421 cgacaacgag tggacggtcg gtatgacacg cgtggctcgc ggcgagatca tcttcttcag
     3481 catggacgag gagagcaccg tcatccagga gcatctccgg gagcgcggac gcgccgtcgt
     3541 cctcaagaag acgccgcgtg gcgagatgat cacactcctt gagcaccggc gggagacatc
     3601 gattctcctc gccagccaga tcccggcgac gttcgagggt cgtgcccgcg tcaatatcca
     3661 gaacgcgatg gcggccacgg cggcggccgt ggcggacgac atccagctcg atcacatccg
     3721 ccaggcgttg cggaccttta ccagcaactt ctcgcagacc ccgggtcggt tcaacctgct
     3781 cgacatcgcg gggcagcggg tcctgatcga ctattgtcat aacgtcgcgg gactcgaggc
     3841 gattgccgat ttcgtggagc gcatggcggc ggaccatacc gtcgccgtga tcagcatgcc
     3901 cggcgatcgc tcggatggcg atatcgaggc gttcggcgcg ctggcggcga agtccttcga
     3961 ccagctgatc attcgtgagg acgacaacac ccgagggcga ccgagcggtc agatctcgca
     4021 gatgttgtat gacgctgcgg ttcggggcgg gatcgaccca accaacgtga agatcatcgt
     4081 caatgaactc gaggcgtgcc gcgtggcggt cgaagaagcc gacaagggcg atctcgtggt
     4141 gctgatggcg gacaagccag tgaaagtctg ggaaatgctg acgaacagcg gcgagccggt
     4201 ctaggcggcc ctccggggcg aaagcggcgg gacctccgtg tcccgtcgtc cggacgaccg
     4261 attccccgtc gtagcggatc accccgtgtg acccatcggg cgcagcccga tatc
//
LOCUS       DASTMD010000816         3877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1326502, whole genome shotgun sequence.
ACCESSION   DASTMD010000816 DASTMD010000000
VERSION     DASTMD010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326502"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..634
                     /locus_tag="VFQ54_12100"
     CDS             209..634
                     /locus_tag="VFQ54_12100"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0165777.1"
                     /translation="MRFMMMIYPGEKAENGTLPDPSMFEAMLKYNQELQQAGVLISLD
                     GLQPTAKGARVTFTGGKATVTDGPFTETKEVIGGYWMIRVNSRDEAIAWATRVPCAED
                     EVIELRQIYDLGDFPEEAVSPDLLAREKELGEKLPDQPW"
     gene            775..1890
                     /locus_tag="VFQ54_12105"
     CDS             775..1890
                     /locus_tag="VFQ54_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165778.1"
                     /translation="MGGVRVLVGTRKGAFILMSDEKREQWDVSGPHFAGWEIYHLNGS
                     PVDPNRLYASQSSGWFGQMIQRSDDGGKTWDPVGNEFNYDGVPGTHQWYDGTQHPWKF
                     ARVWHIEPSLTDVDTVYAGVEDAALFRSQDGGKSWHELSGLRGHDTGPLWQPGAGGMC
                     LHTIILDPVDPDRIFIAISSAGAFRTDDGGKTWLPINKGLRSGEIPDQDAEVGHCVHN
                     LTMHPSKPGHLFMQKHWDVMRSDNAGDSWHEVSGNLPSDFGFPIAVHAHEPETIYVVP
                     ITSDSLHFPPEGKLRVYRSRSGGNEWEALTRGLPQENCYVNILRDAMAVDTLDSCGIY
                     FGTTGGQVYASSDSGDSWMPIVRDLPAVLSVEVQTLS"
     gene            1887..2183
                     /locus_tag="VFQ54_12110"
     CDS             1887..2183
                     /locus_tag="VFQ54_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU0165779.1"
                     /translation="MIRIMLPAHLRTIARVPGEVELEIDAPVTPRKVVDAIELAYPTL
                     RGTIRDSATKERRQLVRFFACEQDISHDSQDAPLPDEIVNGREPFLIIGAMSGG"
     gene            2776..3342
                     /locus_tag="VFQ54_12115"
     CDS             2776..3342
                     /locus_tag="VFQ54_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU0165780.1"
                     /translation="MRKVVVSMFMTVDGVMEAPNEWSFQFWSDESARYKFDELFEGDA
                     LLLGRVTYEGFAAAWPTMTDEQGFADRMNGLPKYVATTTLDDLTWNATRIEGDIAESV
                     ATLKAQEGQNILVFGSADLVNYLTDHDLVDEYRLMIFPVVVGKGKHLFAEGLGKKAFR
                     LAASKVFASGVVVQTYAPAAVDGADDAV"
     gene            3461..>3877
                     /locus_tag="VFQ54_12120"
     CDS             3461..>3877
                     /locus_tag="VFQ54_12120"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0165781.1"
                     /translation="MDTALIAPKFSRERLGRMNEIMASHVERGTTPGLITAVSRKGET
                     HVEVIGSMEIGGAPLQRDSIFRIASMSKPITAVATLILLEECVLRLDEPVDRLLPELA
                     DRQVLRSLESEATDTVPANRAITLRDLLTFTFGQGLV"
BASE COUNT          748 a         1233 c         1217 g          679 t
ORIGIN      
        1 gatttctgcc gccaacttgg acggttcgag gaggcccgcg cctcctacga gcgtgcgctc
       61 gcgctcagcc tgcaggaacc ggaacggcga ttcctcgaag ggagacttcg cgacctggct
      121 gcagaggcat gatgacctgc gacagtgccc gaaatcgctc ttgaaccgac gcccgacgac
      181 acccggcttc acataaggag atgacacgat gcgattcatg atgatgatct accccgggga
      241 gaaggcggag aacgggacgt tgcccgaccc gagcatgttc gaggccatgc tgaagtacaa
      301 ccaggaactc cagcaggcag gtgttctgat ctctctcgat ggtctccagc caacagcgaa
      361 gggcgcccgc gtcaccttca ccggtggcaa agccacggtc accgacggtc ccttcaccga
      421 gacgaaggaa gtcatcggcg gctactggat gatccgggtg aactcgcgcg acgaggcgat
      481 cgcatgggcg acacgcgttc cctgcgccga agacgaggtg atcgagctgc gccagatcta
      541 cgatctcggg gattttccag aagaagccgt ctcccccgac ctgctcgccc gggagaagga
      601 gttgggcgaa aaactgccgg atcagccctg gtagttcccg catgccaccg gcaggtcgcc
      661 ggtggcgatc gctgcgctca ctcccgcgac gagcgctgat gtcacggaac gttgatcggc
      721 gtccctttcc ccatcggggc attcgacaag acgatacgcg agaaggagtc agcaatgggc
      781 ggagtacgag ttctggttgg aacgcgcaag ggcgcgttca tcctgatgtc ggacgagaag
      841 cgcgagcagt gggatgtctc cgggccgcat ttcgcgggct gggagatcta ccatctcaac
      901 ggatccccgg tcgatccgaa ccgcctctat gcgtcgcaat cgagcggctg gtttggccag
      961 atgatccagc gctcggatga tggtggcaag acgtgggacc cggtcggaaa cgagttcaac
     1021 tacgacggcg ttccgggaac gcatcagtgg tacgacggga cacagcatcc ctggaagttc
     1081 gcccgcgtct ggcacatcga gccgtcgctg acggacgtcg acaccgtcta cgccggggtc
     1141 gaggatgccg cgctcttccg ctcgcaggac ggcggcaaat cctggcatga gctctccgga
     1201 cttcgcggtc acgacaccgg tccgctctgg cagccgggag ccggcggcat gtgcctccac
     1261 acgatcatcc tcgatccggt cgatcccgat cgcatcttca tcgcgatctc gtcggccggc
     1321 gccttccgta ccgacgatgg cggcaagacc tggttgccga tcaacaaggg acttcgctct
     1381 ggcgagattc cggatcagga cgcggaagtc ggtcactgtg tccacaacct gacaatgcat
     1441 ccgtcgaagc cgggtcatct cttcatgcag aagcactggg atgtgatgcg gagcgacaac
     1501 gccggtgatt cctggcacga ggtgagcggc aacctgccca gcgacttcgg gttcccgata
     1561 gccgtgcacg cacacgagcc ggaaacgatc tacgtcgtgc cgattacgag cgactcgctt
     1621 cacttcccgc cggaaggaaa gctccgcgtc taccgcagcc gctccggtgg caacgagtgg
     1681 gaagccctca cacggggctt gccgcaggag aactgctacg tgaacatcct gcgcgacgcg
     1741 atggcggtcg acacgctcga ttcatgcggc atctatttcg ggacgaccgg tggccaggtc
     1801 tacgcgtcgt cagattcggg agacagctgg atgcctatcg tgcgcgatct ccccgctgtc
     1861 ctttccgtgg aagtgcagac actctcatga tccgcatcat gcttcccgcc cacctgcgga
     1921 cgattgcccg cgttcccggc gaggtggaac tcgagatcga cgcgccagtg acaccgcgca
     1981 aggtggtcga tgcgatcgag ctcgcctacc cgacgctgcg cggcacgatc cgcgacagcg
     2041 cgacgaagga acgacgccag ctcgtgcgtt tctttgcgtg cgagcaggac atctcccacg
     2101 attcccagga tgcgccgctg ccggacgaaa tcgtgaacgg ccgcgagccg ttcctcatca
     2161 tcggcgcgat gtccggcggt tgatcgacgg cccacacacc atgcgagagg cgggacaggc
     2221 cgtccggacc ttcggtccgg cagcggcggt cccgccccac gaacgaccgc tatgcgtaat
     2281 ccggatgttc gtacgggagg acctccgtgt cctccctccg aaacgcatcc acttccggca
     2341 gatgtacatg ccggaacgcc cccaacgtct tcccgaggga ccagtctagc accgtgtgct
     2401 tcccgcacga cgcttcacaa aacatgtcct cccgagcttg tcgagggatc agccggcgtc
     2461 cacatgctcc cggcacgacg cgcttcccga agaagcgcag ataggcgaga cgtcacgcat
     2521 gggacgtggt ccctcgactg cgcttcaccg cacgcctgac ggcgctgcgg ctgcgctccg
     2581 ctcgggatga cgaggaggcg tcgcgtcatc tgtacgtgcg cgccctcctc acatattccc
     2641 cgctcttcct ccgacgccaa tgtcgaattc gaccgcatcc gatcgaccac ctgatgtcac
     2701 cgcccggagc gaccggtgcg gagaacggtc cgatcgcggg tggcaacacc cggttgccca
     2761 tgaaggagcc cggagatgcg caaggttgtg gtttcgatgt tcatgacggt cgatggcgtg
     2821 atggaagctc ccaacgagtg gtccttccag ttctggagtg acgaatccgc ccggtacaag
     2881 ttcgatgagc tcttcgaggg cgacgccctc ctgctgggac gcgtgaccta cgagggcttc
     2941 gcggcagcgt ggccaacgat gaccgacgag caggggttcg cggaccgcat gaacggcctc
     3001 ccgaaatatg tggcgacaac gacgctcgat gacctgacct ggaatgcgac gcggatcgaa
     3061 ggcgatatcg ccgaatcagt cgccaccctg aaagcgcagg aagggcagaa catcctcgtc
     3121 ttcggaagcg ccgatctcgt caactacctg accgatcacg atctggtcga cgagtatcgc
     3181 ctgatgattt tccccgtggt ggtggggaag gggaagcacc tcttcgccga agggttgggg
     3241 aagaaggcgt tccgtctggc cgcgagcaag gtcttcgcat cgggcgtcgt cgtgcagaca
     3301 tatgccccgg cggccgttga tggcgcggat gatgccgtgt agttcgtcgt aaccgcgccc
     3361 cgccaaccgc aagcacggag aagcgaaggc gcgatgaggc cgttaatctg gagagagatg
     3421 gtttcgctca ggttggtagc catgatgaag ggacttgcgt atggatacgg cgcttatcgc
     3481 acccaaattc tcccgcgagc ggctcggccg catgaacgag atcatggcca gccacgtcga
     3541 gcgtggcacg acgccagggc tcatcaccgc ggtcagtcgc aagggtgaaa cgcacgtcga
     3601 ggtgatagga tcgatggaga tcggcggcgc gccgctccag cgtgactcga tcttccgcat
     3661 cgcatcgatg tcgaaaccga tcaccgccgt cgctaccttg atccttcttg aggaatgcgt
     3721 gctccggctc gacgaaccgg tcgaccgcct cctgccggaa ctcgcggatc ggcaggtgct
     3781 ccgatcgctc gaatcggagg cgaccgatac ggtaccggcg aaccgcgcga tcacgctgcg
     3841 ggatctgctc accttcacct tcggtcaggg cctggtc
//
LOCUS       DASTMD010000817         1926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2754883, whole genome shotgun sequence.
ACCESSION   DASTMD010000817 DASTMD010000000
VERSION     DASTMD010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2754883"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..676
                     /locus_tag="VFQ54_12125"
     CDS             134..676
                     /locus_tag="VFQ54_12125"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU0165782.1"
                     /translation="MSQFEPTLVLPVGERDHASGPVDAPVTLVEYGDYQCPYCGRAYP
                     IVKAVQEALGDNLRFIFRNFPITTSHEHAQHAAEVAEAAAAQGKFWEMHDLLFENQDA
                     LDDDHLRGLAQQLGLDLARFEADMTGHHFAERVREDFMSGVRSGVNGTPTFFINGARF
                     DGSWEQESLTAALEDAARIS"
     gene            complement(875..1573)
                     /locus_tag="VFQ54_12130"
     CDS             complement(875..1573)
                     /locus_tag="VFQ54_12130"
                     /inference="COORDINATES: protein motif:HMM:NF006119.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0165783.1"
                     /translation="MTHINGSVAFVTGGNRGIGKRTVDELLARGAAKVYATARNPQPS
                     EDPRIVVLQLDVTDKASIAAAAAEAQDVQILINNAGILGAGLSTLGVDLDDARAVFET
                     NVFAPLAVTQAFAPILAKNGGGVVVNIHSALSWAAGWGAVGASKAAIWSFSNSLRVDL
                     EGQQTRVIGVHLGYTDTDLVAALDVPKNDPRDIARAIADGIENDEVEVLADDTAKYVK
                     SLLSGPVTALELNR"
     gene            complement(1608..1778)
                     /locus_tag="VFQ54_12135"
     CDS             complement(1608..1778)
                     /locus_tag="VFQ54_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165784.1"
                     /translation="MSIEKSGAIPESRQRREDLPELTNTQDRYLEPIWLVTAIFGHQI
                     RKELDAIAASRS"
BASE COUNT          341 a          653 c          584 g          348 t
ORIGIN      
        1 gcaaacgggc cctcttcgtg atcgatgccg agggcgagat cgcctggagc tacgtctcgc
       61 caaccggagt caatcccggc gccgacggca tcctccgcgc actcgacgat cttcaggcga
      121 agggaaccgc atcatgagcc agttcgaacc aactctcgtt cttcccgtcg gcgaacgcga
      181 ccacgcctcc ggcccagtcg acgcaccggt gacgctcgtg gaatacggcg actatcagtg
      241 cccctactgc ggtcgcgcct atccgatcgt gaaggcggtg caggaggcgc tcggcgacaa
      301 tcttcgcttc atcttccgca acttcccgat cacgacctcg cacgagcacg cacaacacgc
      361 ggcggaggtc gcggaggccg ccgccgccca aggcaagttc tgggagatgc acgatctcct
      421 cttcgagaat caggacgcgc tcgacgacga ccacctgcgc ggcctggcgc agcaactcgg
      481 tctcgacctc gcgcgcttcg aagccgacat gaccggccac cacttcgccg agcgcgtccg
      541 cgaggacttc atgagcggcg tccggagcgg tgtgaacggc acgccgacct tcttcatcaa
      601 cggcgcccgc ttcgacggaa gctgggagca ggaatccctg acggccgcac tcgaggacgc
      661 ggcacgtatc tcctgatccg gactattcga cgtcgcgata tatgacacgc cgaatgaccc
      721 acggtaccga gtttgggatg gcgggtcgta gcgtggggcc gaggcccgct caacccagga
      781 cggattccgg aatcattgcc ccgcttgctg ggggcggggc tggtagggcg gccgccgcaa
      841 agccggcgca ggagagcatg cgtctcggtc gagcctagcg gttgagctcc agagccgtca
      901 ccggacccga aagcaggctc ttcacatact tcgccgtgtc gtcggccagc acttcgacct
      961 cgtcgttctc gatcccgtcg gcaatcgcgc gtgcgatgtc acgcgggtcg ttcttcggca
     1021 catcgagcgc cgcgacgaga tcggtatccg tgtaaccgag atggacgccg atcacccgcg
     1081 tctgctgtcc ctcgagatcg acgcggagtg agttcgagaa cgaccagatg gccgccttcg
     1141 acgcgccgac ggctccccat cccgccgccc agctgagcgc ggagtggata ttgacgacga
     1201 ccccgccgcc gttcttcgcg aggatcggcg cgaacgcctg ggtcaccgcg agcggcgcga
     1261 agacgttcgt ctcgaagacc gcgcgggcat catcgaggtc gacgccgagc gtcgaaagac
     1321 cagcgcccaa gataccggcg ttgttgatca ggatctggac gtcctgcgcc tccgcggcag
     1381 ccgccgcgat cgatgccttg tccgttacgt cgagctgcag gaccacgatg cgcggatcct
     1441 cgctcggctg cggatttcgc gccgtcgcgt agaccttcgc cgcccctcgg gcgaggagct
     1501 cgtccacggt acgcttgccg atcccacgat tgccaccggt cacgaatgcg actgaaccat
     1561 tgatgtgtgt cacgacgatg tcctttgctg ttgagtgcag ggagtgccta cgatcgggat
     1621 gccgcgatgg catccagttc cttccgaatc tggtgaccga agattgcggt cacgagccag
     1681 atcggctcaa gatatcgatc ctgggtgttc gtgagttcag gcaggtcctc tcgccgttgc
     1741 cggctctccg ggattgctcc cgacttctcg atcgacatag tcaatctcat ttcatctatg
     1801 tcaccctagt tgaccggggg cgatccgtca agtacctttg gcacatgaac gaatcacttc
     1861 ccgccacccg tcgctatgac atccgcccca ctcccgatgg cgttcgcctc gtccaggacc
     1921 tgctca
//
LOCUS       DASTMD010000818         4417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1773748, whole genome shotgun sequence.
ACCESSION   DASTMD010000818 DASTMD010000000
VERSION     DASTMD010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773748"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2147
                     /locus_tag="VFQ54_12140"
     CDS             <1..2147
                     /locus_tag="VFQ54_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU0165785.1"
                     /translation="RLTDYLRPRQIVLLLDNLEHLLPAAPLIANLLDAAPGVTILATS
                     RIRLGIAHEVLYPVLPLEIPTPEQMSSHSRAAASPAIQLFVDRARRHHPAMALTHENL
                     HAVANICRRMDGLPLAIELAAAQLANNPLESIAAELPAITIELARDGDLPDRLRSLNR
                     VVDWSYRLIAPPTQTLFRRLAAFSGGASRDMVNAMMADVMVNPDEVGERLSTLVDANL
                     VFETIQFDQTSRYMMLETIRDYGLGQLALAGETNAVRDRHAACFTRLASRLGPELQSH
                     EVFSARGRFTADLNNLRAGLAWAVAREDPTSITALLEGTLWCWHVAGLWGEALEWHTR
                     AAPIVQERGTPRQRASALLGHGHFLGLHGDPAAAIERLGAAETLFLEISGADGKVGAA
                     QARREIAFQHLNTGDLVLGREAMAESLARYRATTDAWGLAYTLNCLSDFDAHFGRYAD
                     AEAEVQEAIAIANRIGNPWMRGEGLRRLFYIQFYTHPLPKLQSLAQECLGLAYELDHR
                     QFICGSLLQLGLASIRMDDVDAAELAIQESMRIAIEINDERKIPDCLELLAAIATRRT
                     LHAEAARLLGIATVLRAGRAEPVLWNDFMNRQTIDRIVDAIGEDAYLRERTAGEGLSH
                     ADARRIALTSPAARTIQAPSLAIPAQELALLSRREREVLNLVAQGLTDREIAASLFIS
                     RPTASKHVASILTKLGVPSRAAAAALAGRTEP"
     gene            2496..3569
                     /locus_tag="VFQ54_12145"
     CDS             2496..3569
                     /locus_tag="VFQ54_12145"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase family protein"
                     /protein_id="HEU0165786.1"
                     /translation="MSTTTARRMEEFIVITRRTFTRLTAGIAIAAAIPIRASAQTPPP
                     EELELLTLVDTLYNKGWNAHDPNVVFDIIAADHVYNDPITPGIGTGPKGYFDLMTTYL
                     GAFPDINFPIHALVPDTENQIIGVRWSADATQDGPIFGIPATHKHLRVPAMAIHTITE
                     GKISHTFVIYDTLGMVTQLGLIPPTGSNPASTPEAALIVTMDGTPGPILSPALAATKA
                     RDFYNKIWNDQNPAITADLVTPDFQYIDPAVPTLPKGPQGYEALVSSFLTGIPDLTIT
                     IDTVASNGNAVAMHWTGTGTQTGEFLGIPATGKKVTAQAISFLTFKDSLISTNWSVFD
                     ALGILIQLGAIPPLGGGDATPGS"
     gene            3572..3775
                     /locus_tag="VFQ54_12150"
     CDS             3572..3775
                     /locus_tag="VFQ54_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165787.1"
                     /translation="MVTGESSGTTYVVRCECGVVLTDTDEETLIRKMQSHTRTRHRVE
                     FTDEQVKLLVETEIRQRRSIGAA"
     gene            4002..>4417
                     /locus_tag="VFQ54_12155"
     CDS             4002..>4417
                     /locus_tag="VFQ54_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165788.1"
                     /translation="MTFRQHRSARLVVTTVAAFLLGMLAVVALGTFSSPRSALAADSA
                     TPVVTATTTPRLWTLDLVVETQDSKPIPVGAKICLTNTDPSTPYSSCLDVSSGTAHYV
                     DMPFGSYGVTGTGLGHYHLLDGIYIARGFGQPDSWSV"
BASE COUNT          793 a         1561 c         1288 g          775 t
ORIGIN      
        1 gccgtctcac cgattacctg cgaccgcgcc agatagtcct gctgctcgat aacctcgagc
       61 atctgctgcc tgccgccccg ctgatcgcca acctgctgga cgcggcaccg ggtgtgacga
      121 tcctcgcgac cagccgcatt cgactcggga tcgcgcacga agttctctac ccggttctcc
      181 cgctcgaaat cccgaccccg gagcagatga gcagccactc cagagccgcg gcctcgcccg
      241 cgatccagct cttcgtggat cgcgctcgcc gtcaccaccc ggcgatggcg ctgacgcacg
      301 agaatcttca tgccgtcgcc aacatctgcc ggcgaatgga cggccttccc ctcgcgatcg
      361 agctcgccgc cgcgcagctt gcgaacaatc cgctcgagag catcgccgcc gaactcccgg
      421 cgatcacgat cgagctggca agggatggcg acctcccgga tcgcctccgc agcctgaatc
      481 gcgtcgtcga ctggagctat cgcctgatcg cgccacccac gcaaacgctc ttccggcgac
      541 tcgcggcatt ctccggcggc gcctcgcggg acatggtcaa cgccatgatg gccgacgtca
      601 tggtgaaccc ggacgaggtg ggtgagcgcc tgtcgacact cgtcgatgcc aatctcgtct
      661 tcgaaacgat ccagttcgat cagacatcac gctacatgat gctcgagacc attcgggact
      721 acggactcgg tcagctcgca ctcgccggcg agaccaacgc cgtccgcgat cgccatgccg
      781 catgtttcac ccgcctggcg agtcgcctcg ggccggaatt gcagagccac gaggtctttt
      841 cggcccgtgg tcgattcacg gcggacctca acaaccttcg cgccgggctc gcctgggccg
      901 tcgcccgcga ggacccgacg tcgatcactg cgctcctcga agggacgttg tggtgctggc
      961 atgtcgccgg tctctggggt gaagccctgg agtggcacac ccgtgccgct ccgatcgtgc
     1021 aggaacgggg aaccccacgg cagcgggcct cggcgctgct cggacacggg catttcctcg
     1081 gcctgcacgg ggatccggcg gcggcgatcg agcgcctggg tgccgccgaa accctcttcc
     1141 tggagatcag cggcgccgac ggcaaggtcg gggccgcgca ggcgcgccgg gaaatcgcct
     1201 ttcagcatct caataccggg gatctcgtcc ttgggagaga agcgatggcc gaatcgctcg
     1261 cgcggtatcg cgcgaccacc gatgcgtggg gactcgccta caccttgaac tgtctttccg
     1321 acttcgatgc ccatttcggt cgctacgccg atgccgaggc ggaagttcag gaggcaatcg
     1381 ccatcgcgaa ccggatcggc aatccctgga tgcgcgggga aggactccgg cgtctcttct
     1441 acatccagtt ctatacgcac ccactgccga agctgcagtc gctggcgcag gaatgtctcg
     1501 gtctcgccta tgaactcgat caccggcagt tcatctgcgg gtctctgctt cagcttgggc
     1561 tcgcctcgat caggatggac gacgtcgacg ccgccgaact ggcgatccag gaatcgatga
     1621 ggatcgcgat cgagatcaac gacgagcgga agattcccga ttgcctcgag ctcctggcgg
     1681 ccatcgccac gaggcggacg ctccatgcgg aggccgcgcg tctccttggc atcgcgacgg
     1741 tgttgcgggc aggtcgtgcc gagccggtgc tctggaacga tttcatgaac cggcagacca
     1801 tcgaccgcat cgtcgacgcg atcggcgagg acgcgtatct ccgggaacgg accgcgggcg
     1861 aaggtctctc ccatgccgat gcccgccgca tcgccctgac ctcaccggcc gcacgcacga
     1921 tccaggctcc ttcgctcgcg ataccggcgc aggagctggc gcttctcagc cgcagggagc
     1981 gggaagtact caacctcgtg gcgcagggat tgaccgaccg ggagatcgcc gcgtcactct
     2041 ttatctcgcg ccccacggcg tccaagcacg tcgcctcgat cctcaccaag ctgggagtcc
     2101 cgagccgagc cgccgctgcc gcgctcgccg ggcgcaccga gccctgatcc tcccgcccga
     2161 tcaatcctcc ggcagaccat cccgcgcacc ccgggttcga gaacccgggg aagtcattgc
     2221 atgccgggaa cagaatcttg cctttccatg ataattcgtc gttcttcctc attcagcgcg
     2281 gaaatccacc ccctggaacc tcaggaaatg gtgagaactc accatatgcg gtgccacgcg
     2341 gtcaccctac gatcgtattg gcaatcagcg agaccgctat tcgcgatatc accgccgtcg
     2401 ccctggtcct cgcgctacgc gtgtgagatt ccacgcgcgg atccatgccc cggtcgggtc
     2461 ccacgtctgt tcgcaatcca ccacatgatc ccgacgtgtc caccacaacg gcgcgtcgaa
     2521 tggaggagtt catcgtgatt acccgtcgca ccttcacccg actgaccgcc ggcatcgcca
     2581 tcgcggcagc catcccgatc cgcgcctcgg cccagacgcc gcctcccgag gaactcgagc
     2641 tcctcaccct cgtcgatacc ctctacaaca agggctggaa cgcgcacgac ccgaacgtgg
     2701 tcttcgacat catcgcggca gatcacgtct ataacgatcc gatcacgccg ggaatcggta
     2761 ccggccccaa gggctatttc gacctgatga cgacctacct cggtgcgttc ccggacatca
     2821 atttcccgat ccacgcgctc gtccccgaca cggagaacca gatcatcggc gtgcggtggt
     2881 cggcagatgc gacgcaggat ggcccgatct tcggaattcc ggctacgcac aagcatctcc
     2941 gggtgccggc gatggccatt cacacgatca cggaagggaa gatctcgcac accttcgtga
     3001 tctacgacac cctcggcatg gttacccagc tgggcctcat cccgccgacc ggatcgaacc
     3061 cggcctccac tcccgaagcg gcgttgatcg tgaccatgga cgggactccg ggccccatcc
     3121 tttcccccgc tctcgccgcg acgaaggcca gggacttcta caacaagatc tggaacgacc
     3181 agaatcccgc aatcaccgcg gatctggtta ccccggattt ccagtacatc gaccctgcgg
     3241 tgccaaccct gcccaaaggt ccgcaggggt atgaggctct cgtctcgtcc ttcctcaccg
     3301 gaattcccga tctcacgatc acgatcgata cggttgcctc gaacgggaac gccgtcgcca
     3361 tgcactggac gggtaccgga acccagaccg gggagttcct cggcattcct gcgaccggga
     3421 agaaggtgac cgcccaggcg atcagcttcc tgaccttcaa ggattcgctg atctcgacga
     3481 actggagtgt cttcgacgcc ctgggaatcc tgattcagct cggcgcgatc ccgcccctcg
     3541 ggggcggcga cgccacaccc ggctcctgac gatggtgacc ggagagagca gcgggacgac
     3601 ctatgtcgtg cgctgcgaat gtggggtcgt gctgaccgat acagacgaag aaaccctcat
     3661 ccgcaagatg caatcacata cgcgcacgcg tcaccgggtg gaattcacgg acgagcaggt
     3721 gaagctgctg gtcgaaacag aaatacgaca acgacgatcg atcggcgcgg cgtaacgacc
     3781 gcgagtgtgg tgtggccgcg tggggaaccg ggagcggttc ccccgcacgg tcgagcccgg
     3841 aatcgacgtg ttcgctgcgc ctccgggccg gtgatgcctg accgcgccct tcatgacacc
     3901 cgccatctcg attgccacgg gagcgtgtcc ggtcgccagc tgccagatag caccctgcct
     3961 cgttcgcccg tcacctttca gtgggtatgt ggaggataca gatgacattt cgccaacacc
     4021 ggtccgcacg cctcgtcgtg acgaccgtcg ccgcgttcct gctcgggatg ctcgccgtcg
     4081 tcgcactcgg cacgttctcc tcaccgcgat cggcgcttgc cgccgactcc gcgaccccgg
     4141 tcgtcaccgc gaccaccacg cccaggctct ggacgctcga tctcgtcgtg gagacgcagg
     4201 attcgaagcc gatacccgtc ggcgccaaga tctgcctcac gaataccgat ccctcgacgc
     4261 cctattcctc ctgtctcgat gtctcgagcg gaaccgcgca ttatgtcgat atgccattcg
     4321 gctcctacgg cgtcaccggc accgggctcg gtcactacca tctgctcgac ggaatctaca
     4381 tcgcccgtgg cttcgggcag ccagactcct ggtcggt
//
LOCUS       DASTMD010000819         3583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1417254, whole genome shotgun sequence.
ACCESSION   DASTMD010000819 DASTMD010000000
VERSION     DASTMD010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417254"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VFQ54_12160"
     CDS             complement(<1..715)
                     /locus_tag="VFQ54_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0165789.1"
                     /translation="MEERVEQFLSSLETERGFSPNTIAAYRNDLQQFGAYMADPPADD
                     QIDAISDWSELNAHAVGGYLLHLRERSYAASTIARKTAALKSFCGFLNREGVTPEDIG
                     ARLSSPKVDRYVPRAISHDEIDQLLAQPLKDGSTKPEAIRDWAMLEVLYATGMRVSEL
                     VSLNLADVDMPGQQALCQGKAGRKRTVPLTQRSLIAVSAYIENARSILTDAEATPLFV
                     NHRGGRLTRQGFWLILKAYA"
     gene            complement(818..1945)
                     /gene="ald"
                     /locus_tag="VFQ54_12165"
     CDS             complement(818..1945)
                     /gene="ald"
                     /locus_tag="VFQ54_12165"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU0165790.1"
                     /translation="MIVGVPTEIKDRESRVSTTPAGVHEYVARGHRVLVETGAGDGSG
                     FADSDYAAAGGEIIPTHEGVFAAADMIAKVKEPTHDEYNLLRENQLLFTYLHLAADEP
                     LTRALMERKVQSIAYETVQSGNGPLPLLTPMSEVAGRMSIQVGAYYLQRPNGGRGVLL
                     GGVPGVPAADVVVIGGGVVGTNAAQIALGMGANVTILDRNIERLRFLDQILHGRLHTQ
                     ASNTHAVAEAVAKADLVIGGVLIPGARAPKLVTAQMVKTMRPGSVMVDVAIDQGGCFE
                     TSKPTSHSNPVYEVDGVIHYCVTNMPGAVSRTSTFALSNVTLPYGLALADHGLAVATR
                     LDPALAKGVNVLNGQVTHPGVAEALGLPYVDLTAALGTLAA"
     gene            2460..>3583
                     /gene="rseP"
                     /locus_tag="VFQ54_12170"
     CDS             2460..>3583
                     /gene="rseP"
                     /locus_tag="VFQ54_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU0165791.1"
                     /translation="MFHISEGLLLGLLIIPILAFLIIIHELGHFFAARSVGVTVEEFG
                     IGLPPRAKGWRWKGVLWSLNWIPFGGFVRVKGEDGSDLEPGSMNSKKPWQRAWFLIAG
                     PLMNFIAAIVLSIILIGFQGLPSETNHIYIGQVSSNSPAQIAGWQPGDSITAVEGQPI
                     TSTSQLQSLIKSYAGKPVAITIKRGDETIETTVTPRKNPPANQGATGITMGEAIVSGI
                     HFTDVDQGSVAEKAGLKHGDTLVSVDGVSIEAAQQGTSLLQNSQGKTVPIVVKRGGKD
                     VSLSLPVPTLSLLLTAVAKDSPAADAGLFGGDHVVSVNGTPIPDGASFQSALIANSGK
                     TVTVGIVRNVVLDNGGTQTKNLEVKLEVPKIGGSDNPVGVV"
BASE COUNT          636 a         1131 c         1155 g          661 t
ORIGIN      
        1 gcgcgtacgc cttgaggatc aaccagaacc cctggcgtgt caggcgaccg ccacggtgat
       61 tgacgaagag cggtgtcgct tcggcatcgg tcaggatgga gcgggcattc tcgatgtagg
      121 ccgaaacggc gatcagcgat cgctgcgtca acggaaccgt tcgcttgcga ccggccttgc
      181 cctggcagag cgcctgctgg cccggcatgt cgacatcggc gaggttgagc gagacgagtt
      241 cgctgacccg catcccggtt gcatagagga cttcgagcat ggcccagtcg cggattgctt
      301 ccggcttcgt cgaaccgtcc ttcaatggct gggcgagcaa ctggtcgatt tcatcgtggg
      361 aaatcgcgcg cggcacgtag cggtcgacct tcggcgagct caggcgcgcg ccgatatctt
      421 ccggcgtcac cccttcgcgg ttcaggaagc cgcagaacga cttcaatgcc gccgtcttcc
      481 gggcgatcgt ggaggcggca taggatcgct cgcgaagatg gaggaggtat ccgccaacgg
      541 catgggcgtt caactccgac cagtccgaga tcgcatcgat ctgatcatcg gcgggtggat
      601 ctgccatata ggcaccgaac tgctgcaagt cgttgcgata ggcagcgatc gtgttgggag
      661 agaatccccg ttccgtctcc agagagctca ggaactgctc gacgcgctcc tccatccgta
      721 tggtttcctt tcaatgcgaa cacagcatca gatcagcacg gcggcgtggc tgtacgcggg
      781 agatttcaat ccggactacc gaccgaacta ccgaagttta cgctgccagc gtcccgaggg
      841 ccgccgtcag gtcgacatat ggtagcccga gcgcctccgc gacgcccgga tgagtcacct
      901 gcccgttgag cacgttgacc cccttcgcca gcgccggatc gagccgcgtg gccacggcca
      961 ggccgtgatc ggcgagggcg agcccgtacg gcagcgtgac gttcgaaagc gcgaacgtac
     1021 tggtgcgtga gaccgcaccc ggcatgttcg tcacgcaata atgaatcacg ccgtcaacct
     1081 cgtagacggg attgctgtgc gacgtcggct tcgacgtctc gaagcagcca ccctggtcga
     1141 tcgcgacgtc gaccatgacg ctgccaggcc gcatcgtctt caccatctgc gcggtgacga
     1201 gcttcggcgc tcgggcgccc gggatgagta cgccgccgat caccaggtcg gccttcgcga
     1261 ccgcctccgc caccgcgtgg gtgttcgacg cctgcgtgtg cagccggccg tggaggatct
     1321 ggtcgaggaa gcgcaggcgc tcgatgttgc gatcgagaat cgtcacgttc gcacccatcc
     1381 cgagggcgat ctgggccgcg ttggtgccga ccacgccacc gccgatgacg acgacgtcgg
     1441 cagcgggaac tcccgggaca ccgccgagaa gcacgccgcg gccaccattc ggtcgttgca
     1501 agtagtaggc gccaacctgg atcgacatcc gaccggccac ctcgctcatc ggggtcagga
     1561 gcggcagcgg tccgtttccg gactggacgg tctcgtacgc gatcgactgg accttgcgct
     1621 ccatgagggc gcgcgtgagc ggctcgtcgg cggcgagatg gaggtaggtg aagaggagct
     1681 gattctcacg gaggaggttg tattcgtcgt gagtgggttc cttgaccttg gcgatcatgt
     1741 ccgccgcggc gaagacgcct tcgtgggtcg ggatgatctc gccgccggcc gccgcatagt
     1801 cactgtccgc gaagccgctg ccatcgcccg ccccggtctc gacgaggacc ctgtggccac
     1861 gcgcgacata ctcgtggacc ccggccggtg tcgtggaaac gcggctttcg cgatccttga
     1921 tctctgtagg cactccgacg atcatccaac tctcctcggt gaaattcacg tgattgacag
     1981 acgcggcctg ccgactggcg aggcacttcc gggggagggc gtcgatcccc tcttcgatgc
     2041 tcccggttcg cgagcggacg catcatagca cgacaaactt gactcgttat gttctttctc
     2101 gagaccgggc tccgatccat tccaggcagt gacaaacgaa gtggtcgcga gttgtcacgc
     2161 gtgtaacctg tcgccgccac gacccttcgc ttcgctggtg tccctgcacc cgatcgccgg
     2221 gcaccggata cctccgcacc gcctgaaggc ggtgtcgacc agtatcgccg ccaggcgttg
     2281 gccggtcgtg acaggaccac gggaacgggc gtgcacgcga tcgcgtgcac catgccggac
     2341 aggatcggtc ggagtccggc gggaggcgga gtgtaccatt agcggtgtgc tgatgctctc
     2401 atgaccgcgc gaggcggtcg attcccgcca gtcagccgca atgatggaga aatgcatccg
     2461 tgttccacat ctcggaaggt ctgctcttgg gccttctgat catcccgatt ctcgccttcc
     2521 tgatcatcat tcacgaactt ggccacttct tcgccgcccg cagtgtcggg gtcacggtcg
     2581 aggaattcgg catcggtctg ccgccgcggg ccaagggttg gcgctggaag ggtgtcctct
     2641 ggtccctcaa ctggatcccg ttcgggggct tcgttcgcgt caagggtgag gacggctccg
     2701 atctcgagcc cggctcgatg aactcgaaga agccgtggca acgcgcctgg ttcctcatcg
     2761 ccgggccgct catgaatttc attgcggcga tcgtcctctc gatcatcctg atcggcttcc
     2821 agggactgcc gtccgaaacc aaccacatct atatcggtca ggtttcgtcg aactcgccgg
     2881 cgcaaatcgc gggctggcag cccggggatt cgatcaccgc ggtcgagggc cagccgatca
     2941 catcgacgag ccagctccaa tcgttgatca agtcctacgc ggggaagccg gtcgcgatca
     3001 cgatcaagcg cggtgacgag acgatcgaga cgaccgtgac gccgcgcaag aatcctcccg
     3061 ccaatcaggg ggcgaccggc atcacgatgg gcgaggcgat cgtctccggg attcacttca
     3121 cggatgtcga ccagggctcg gtggcggaaa aggctggtct gaagcacggc gacacgctgg
     3181 tctcggtcga cggtgtctcg atcgaggccg cgcaacaggg aacgagtctc ctccagaact
     3241 cccagggcaa gacggtgccg atcgtcgtga agcgcggcgg caaggacgtc tcgctctccc
     3301 tcccggtgcc gacgctgagt ctcctgctca ccgcggttgc caaggattca ccagcggccg
     3361 atgcgggact cttcggcggc gatcacgtcg tttccgtgaa tgggacaccg attcccgacg
     3421 gtgcgagctt ccagagtgcg ttgatcgcga acagtggcaa gaccgtcacc gtcggtatcg
     3481 tgcggaatgt cgtgctcgac aatggtggaa cgcagacgaa gaatctcgag gtcaagctcg
     3541 aggtaccgaa gatcggtggc agcgataatc ccgtcggcgt ggt
//
LOCUS       DASTMD010000820         5249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2935100, whole genome shotgun sequence.
ACCESSION   DASTMD010000820 DASTMD010000000
VERSION     DASTMD010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2935100"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1075)
                     /locus_tag="VFQ54_12175"
     CDS             complement(89..1075)
                     /locus_tag="VFQ54_12175"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165792.1"
                     /translation="MTAESAGRRREVVSRLVVDRRPQLLAALIVITIVMSVISPFFFR
                     LDNLLSMTQYGVVVGVLAIGQALVILGGGGGIDLSVGGQLSFIGIVMAAMTMGGVSPW
                     MAAGCAIVLGACIGAINATLIAIFKLPALIVTLGTQFLFASLALVVSAGNSIGVFSAD
                     GFPFLGQGGLIGIPVQVLLVLLPLFVVVIWIQSRTTLGQVLFQVGLNSRAASLVGVRP
                     STVRFFLYVISGVLAAVGAIVTNSWLQTASPTAGDGYELQSITVAVLGGISIAGGRGR
                     LTGVLLALLIVVVLDSGLQLANVGNTVQGGILGIVLIIALLVNFILNRRTST"
     gene            complement(1080..2060)
                     /locus_tag="VFQ54_12180"
     CDS             complement(1080..2060)
                     /locus_tag="VFQ54_12180"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165793.1"
                     /translation="MEIPRVRLGVRVAGLARALLLPILLVVEIGFFGATAPHFFSWGN
                     FSNIIVSSSCLAILASGMTFIILLGGIDVSAGSVLGVVAWTAAKLSSEGVPAAVTFMV
                     AIVVGAALGSLNGVLVILARIPPIIATLGTGALFATLQFLLWNSVDIYSGPIVPFLSK
                     AAISGFPIIAVVIVVVYAVIFYLLRRRVWGRHLYAIGNDHEGARLLGIAVNRVTFSAY
                     VAMGALAGLAGLIYVGLYGTVQSASGTEFTLIAIAAVIVGGSSVLGGEGGVLRTLGGV
                     AFIALAQNAATLIGVPSLWDNVIVGVALLLAVGVDVAGRRLSTLRQNETR"
     gene            complement(2066..3538)
                     /locus_tag="VFQ54_12185"
     CDS             complement(2066..3538)
                     /locus_tag="VFQ54_12185"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEU0165794.1"
                     /translation="MSKSFGPTRALEDVSFTAATGERLAILGENGAGKSTLIKIIAGV
                     YRPDRGTILVDGEPYEPASPAEALDRGISVVYQEPSFFPRLSVLDNVYAGRELRDRLG
                     QVDTRRMRREGAILFDELQLPSALLGRQMGSLSLAEQQLVLIARALHVNSAILILDEP
                     TSILTGSESERLFEAVDRLSLRGVTVLYITHRFDELERVADRFLVLKDGMLVGDLTSS
                     TDRGELLRLMSGRQIIADVHRGDTQVAPGAPALEAKGIVVPGLLADVNLTFRRGEVVG
                     LYGLIGSGRTEAMMTIYGGIHPKAGRFELDGESYAPRSPRDAIRHGIAYLPEDRKLLG
                     LFPTMSVQTNIGAAVLGRFTGFLGYLRTRALRAMAEGWRESLSIKTSSLDADVMSLSG
                     GHQQKTLFARILAVNPKVVILDEPTRGIDVATKAVIQQRIVELAGEDLAVCVISSELP
                     ELLAIADRIYVLRGGRVVAELTDADLSEEKVLAFALGVHE"
     gene            complement(3570..4586)
                     /locus_tag="VFQ54_12190"
     CDS             complement(3570..4586)
                     /locus_tag="VFQ54_12190"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0165795.1"
                     /translation="MKNKRWGVIASIAAIVVLAGCSSTVATSSTASADKSKIKVAYVS
                     LIQGISYYAAFDKGGQAEAKKLGIKFTGTGPAKADSAEQLRIFNSLVAQGYKGIAIAP
                     VDPTALNSAIAAARAKGVTVITSDADATGSKRQLYVAQASGDALGAQVLDQIAEQMGG
                     KGDYGIVSGAANTTSFNSWIAGAEAEQKKKYPDMHLVGGVQYTLTTADALSDAQNLIT
                     AHPDIKGILALPSSGIPGVAQAIENANLTGKIAMCGFGSPQTALPYVKAGVMKSTVLW
                     DVPQLGALTVWAMNQLITGKGIKASNTIPGFKDPVVYDKANQTLILGKPIVFTKANIN
                     NYDY"
     gene            5007..>5249
                     /locus_tag="VFQ54_12195"
     CDS             5007..>5249
                     /locus_tag="VFQ54_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU0165796.1"
                     /translation="MMESVLAVGDVSHDISVRIPRFPGADEKVLALETAVGTGGVAAN
                     AARAVAQAGVSATLASAIGTDPAGQEIVKDLASSGVD"
BASE COUNT         1006 a         1609 c         1721 g          913 t
ORIGIN      
        1 cgtggtcggg gcccaggtga tgtcgtcacc gagattcatg atggtgtcca atcgcgatca
       61 gaggggatgg gtgtcggctg cccggcgctc acgtgctcgt gcgccgattc aggatgaaat
      121 tcacgagaag ggcgatgatc agaacgatgc cgaggattcc gccctgcacg gtgttgccga
      181 cgttcgcgag ctggaggccg gaatcgagca cgaccacgat gagaagcgcg aggagcaccc
      241 cggtgagcct tccccgtccc cccgcgatgc tgatcccgcc gagcacagcg accgtgatcg
      301 actgaagttc gtagccgtct ccggcggttg gtgatgcggt ctgcaaccag gagttcgtca
      361 ctatcgcgcc gacggccgcg agcaccccgc tgatgacgta gaggaagaag cggaccgtcg
      421 agggtcgcac gccgacgagc gacgccgcgc gtgagttcaa cccgacctgg aagagcacct
      481 gcccgagcgt tgtccgggac tgtatccaga tgacgacgac gaagagcggc aggagcacga
      541 gcagcacctg gaccggaatc cctatgagac cgccctggcc gaggaacggg aaaccatcgg
      601 cgctgaagac gccgatggag ttccccgcgc tgacgacgag agccaacgag gcgaagagga
      661 actgcgtgcc gagcgtcacg atgagagccg ggagcttgaa tatggcgatc agcgtcgcgt
      721 tgatcgctcc gatgcacgcg ccgagcacga tcgcgcaccc ggccgccatc cagggggaga
      781 ccccgcccat ggtcatcgcg gccatcacga ttccgataaa gctcagctgt ccccccacgg
      841 agaggtcgat gcccccgccg ccgccgagga tcacgagtgc ctgcccgatg gcgaggacgc
      901 cgacgacgac tccgtactgg gtcatcgaga gcagattgtc gagtcggaag aagaatggtg
      961 agatcacgct catgacgatg gtgatcacga tcagggcggc aaggagctga ggtcgcctgt
     1021 cgaccaccag tcgactcacc acctcacgcc ggcggcctgc ggattctgcc gtcatacgat
     1081 cacctcgttt cgttctgtcg aagagtggag agccgccgtc cggcgacgtc gacgccgaca
     1141 gccaacagca gggcgacgcc cacgatgacg ttgtcccaga gggacggcac gccgatgagc
     1201 gtcgctgcgt tctgagccag ggcgatgaag gcgacaccgc cgagcgtgcg aaggaccccg
     1261 ccctcgccgc cgagaacgct cgagccgccc acgatgaccg ctgcaatggc gatcagggtg
     1321 aactcggtgc cgctggcgga ctgaacggtg ccgtagagcc cgacgtagat cagtccggcg
     1381 agtccggcga gcgcacccat cgcgacgtag gcgctgaacg tcacccgatt gaccgcgatg
     1441 ccgagaagac gcgcgccctc gtggtcgttg ccgatggcgt agagatggcg accccagacg
     1501 cggcggcgga ggagatagaa gatgaccgca tagacgacga cgatcaccac ggcgatgatg
     1561 gggaacccgg agatcgcggc cttgctgagg aacgggacga tcgggccgct gtagatatcc
     1621 acactgttcc agagcaggaa ctgcagggtg gcgaagagcg caccggtgcc gagggtggcg
     1681 atgatcggcg ggatacgggc gaggatgacg agaacaccgt tcagacttcc gagtgcggcg
     1741 ccgaccacga tcgcgaccat gaaggtcact gccgcgggca caccctcgct ggacagtttc
     1801 gccgccgtcc aggcgacgac ccccagtacc gagccggccg agacgtcgat gccgccgagc
     1861 aggatgatga atgtcatccc cgaggcaagg atcgccaggc aggaggagct gacgatgatg
     1921 ttcgagaagt tgccccagga gaagaaatga ggtgccgtag cgccgaagaa gccgatctcg
     1981 acgacgagga ggatcggcag cagaagtgca cgcgcgaggc ccgcgacgcg cacgcccagg
     2041 cgaacgcgag ggatctccat ggcagtcatt catgcacccc tagcgcaaac gcgagcacct
     2101 tctcttccga caggtcggca tcggtgagct cggcgacgac ccggccgccg cggagcacat
     2161 agatccggtc cgcgatggcc aggagttccg gcagctcgct ggagatcacg cacaccgcaa
     2221 ggtcttcgcc cgcgagttcg acgatccgct gttgaatcac tgctttcgtc gccacatcga
     2281 ttcccctggt cggctcatcg aggatgacca ccttgggatt caccgccagg atgcgcgcga
     2341 acaatgtctt ctgctgatgc ccgccggaga gggacatgac gtccgcatcg agactcgacg
     2401 tcttgatgga aagcgattca cgccagcctt cggccatggc gcgaagcgcg cgggttctga
     2461 ggtatccgag aaatcctgtg aacctgccca gcacggccgc accgatattc gtctggacgc
     2521 tcatggtggg aaacagaccc aggagcttgc gatcctccgg caggtaggcg atcccgtgtc
     2581 gtatcgcatc gcggggcgaa cggggcgcgt agctctcccc gtcgagttcg aacctcccgg
     2641 ctttggggtg gattccaccg tagatcgtca tcatggcctc cgtcctcccc gagccgatga
     2701 gcccgtagag accgacgacc tcgcccctgc gaaaggtgag gttcacgtcg gcgagaagtc
     2761 cggggacgac aatgcccttg gcctcgagcg ccggggcacc cggggcgacc tgcgtgtcgc
     2821 cgcggtggac atcggcgatg atctgcctgc cggacatcag acggaggagt tcgccgcgat
     2881 ccgtcgagct cgtgagatcc ccgacgagca tgccgtcttt gaggaccagg aatcgatcgg
     2941 cgacacgctc gagctcgtcg aatcggtgtg tgatgtacag cacggtgacc cctcgaaggc
     3001 tcagtcggtc gaccgcctcg aagagccgtt ccgactcgga gcccgtgagg atggacgtcg
     3061 gctcgtccag gatcagaatc gccgagttga cgtgcagagc acgtgcgatg aggacgagtt
     3121 gctgttcggc aagactgagg ctgcccatct gccgaccgag cagcgcgctc ggaagctgga
     3181 gttcgtcgaa gagaatggcg ccctcgcgtc tcatcctccg tgtgtccacc tgtccgagcc
     3241 tgtcgcgcaa ctcacgcccc gcatagacgt tgtcgagcac cgagagacgg gggaagaacg
     3301 acggctcctg atagacgacg ctgatgcctc gatccagcgc ctccgcgggc gatgccggtt
     3361 catacggctc accgtccacc aggatggtgc cgcggtcggg gcggtatacg ccggcgatga
     3421 tcttgatgag agtggacttg cctgcgccgt tttcgcccag gatcgcgagc ctctcaccgg
     3481 tggcggcggt gaaactcaca tcctcgagcg cccgtgtggg cccaaacgac ttgctcactc
     3541 ccgatatctc gatcatcgcc ggcgccgact caatagtcgt agttgttgat gttcgccttc
     3601 gtgaagacga tcggtttgcc caggatgagc gtctggttgg ccttgtcgta gacgaccgga
     3661 tccttgaatc caggaatcgt gttggacgcc ttgattccct tcccggtgat cagctggttc
     3721 atggcccaca ccgtgagtgc tccgagctgc ggaacatccc agagcacggt ggatttcatg
     3781 acgcccgcct tgacataggg aagagcggtt tgcggggagc cgaaaccaca catcgcgatc
     3841 ttgccggtga ggttcgcgtt ctcgatcgcc tgcgccaccc cggggatgcc cgacgatggg
     3901 agggcgagta tccccttgat gtccgggtgc gccgtgatca gattctgggc gtccgaaagg
     3961 gcgtcggccg tggtcagcgt gtattgcacg ccgcccacga gatgcatgtc cggatacttc
     4021 ttcttctgct cggcctcggc cccggcgatc cacgagttga acgacgtggt gttcgcggca
     4081 ccggacacga tgccgtagtc gcccttcccg cccatctgct cggcgatctg gtcgagcacc
     4141 tgggccccga gtgcgtcgcc gctggcctgc gcgacataga gctgacgctt gctgcccgtg
     4201 gcgtccgcgt cggaggtgat cacggtgacg ccctttgcgc gggccgcggc aatcgccgag
     4261 ttgagcgccg tcggatcgac aggggcgatg gcgattccct tgtaaccctg ggcgacgagg
     4321 ctgttgaaga tgcgcagctg ctcggccgaa tccgcttttg cggggccggt cccggtgaac
     4381 ttgatcccga gcttcttggc ctcggcctgg ccgcccttgt cgaatgccgc atagtacgag
     4441 atgccctgga tcagggaaac gtaggcgacc ttgatcttcg acttgtcggc agaggctgtg
     4501 ctggacgttg ccaccgtgct gctacagccg gcgaggacga cgatcgcggc gatgctcgcg
     4561 atgacgcccc atctcttgtt cttcataacg gtccttctca tttgagtgaa cggcctcggg
     4621 catccgacgc tcgagctgcc agatgtatgg cgagcgtcat atcgagggcc ccggagggac
     4681 gcgacaacgg tcgatcacgt ccttggtctt gcggatcttc cttggtggaa cggggcccaa
     4741 attagtaagc cagtgtttct gcgatgttac ctcttcgtta atacggtaat ttaaaacgct
     4801 catctgctgc actagaatta cagcgagcgg tgacgcagtc gacataaaaa tagctgatca
     4861 aaggccagta tttgcctgac cttggatcgt gctggtagcc gctgaaaaca ggacgatcaa
     4921 ttttctgctc ttttgagcag aggaggacgg cccatgcgac tgcggtataa acgttgtggg
     4981 atcgggtcac gattcaggat gggaacatga tggaatcggt tctggcggtt ggcgatgtca
     5041 gtcacgacat ctccgtgcga attccacgct tcccgggcgc ggacgaaaag gtcctcgcac
     5101 tcgaaaccgc agtcgggacg ggtggcgtcg ccgccaatgc ggcacgagcg gtggcgcagg
     5161 ccggcgtgtc ggcgacactc gcctccgcca tagggacgga cccggctgga caggagatcg
     5221 tcaaggatct cgcctcctcc ggggtcgat
//
LOCUS       DASTMD010000821         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_164074, whole genome shotgun sequence.
ACCESSION   DASTMD010000821 DASTMD010000000
VERSION     DASTMD010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164074"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..911)
                     /gene="moaD"
                     /locus_tag="VFQ54_12200"
     CDS             complement(159..911)
                     /gene="moaD"
                     /locus_tag="VFQ54_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEU0165797.1"
                     /translation="MPTSTTTIKIQVHYFAMMREMLGTAGETMELPLGSTTKDAFAQI
                     AARVPRLAGLERSIMLMVNETYVRGDHLLQDGDELAFIPPVSGGEHDQVTLRKLFQVT
                     ADVLDPRDVEAAVAGPDVGAIVTFSGTVRDNARGRDVTELDYEAYVPAAEKMLAHIGA
                     EIVDRWPGVRTAITHRTGLLQPGESSVIIACASAHRAEAFEAASYAIDRIKEIVPIWK
                     KEFYGDGSTWIGSEAEYQQEIGRLPRGINPPD"
     gene            complement(929..1159)
                     /locus_tag="VFQ54_12205"
     CDS             complement(929..1159)
                     /locus_tag="VFQ54_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165798.1"
                     /translation="MTTDAKNFTVDDLEYNVVTTLSTLLQSEDVLAKYATDFEEAGKI
                     EIAKIFWTLRNNNRKTAMELRAVLGNFIEQQP"
     gene            complement(1350..1949)
                     /locus_tag="VFQ54_12210"
     CDS             complement(1350..1949)
                     /locus_tag="VFQ54_12210"
                     /inference="COORDINATES: protein motif:HMM:NF037150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEU0165799.1"
                     /translation="MEKTTPTRSRNAAATRAAILASARRAFARVGYDGAGVREIARDA
                     GVTAMLVNRYFGSKENLFAEVIANAMEDPVILAAENLAAPDRAEHFARALVGITTSGT
                     TPLDGFLIMLYSASSERAAEIGREQIEKHHQRVMTGALAGDLAPQRAALVLSLVAGFQ
                     VMRQMMGLPALANADPDVLVRLLTPLFAELIHGEGNASN"
BASE COUNT          375 a          691 c          739 g          439 t
ORIGIN      
        1 aaccgcaagc tcggggcata tccgcgcgcc acgggtgctt gggccatcga caccctggtc
       61 gcggaaatga ggattgggct ggatgcgata actgctccgg cgcgcacttc cgccacgcca
      121 gccatgcctg atctgcggcc accgacctga cgtgcgcttt agtccggagg attgatcccc
      181 ctcggaaggc gaccgatctc ctgctggtac tccgcctcgc tgccgatcca cgtcgagccg
      241 tctccgtaga attccttctt ccatatcggc acgatctcct tgatgcgatc gatcgcatag
      301 ctcgccgcct cgaacgcttc ggcccggtgc gcggaggcgc aggcgatgat cacactcgat
      361 tcgccgggct ggaggagacc ggtgcgatgg gtgatcgcgg tgcggacgcc cggccagcgg
      421 tcgacgattt cggcgccgat atgggcgagc atcttctcgg cagcggggac gtaggcctcg
      481 tagtcgagtt cggtcacatc gcgaccgcgc gcgttgtcac gaaccgtgcc cgagaaggtg
      541 acgatcgcac cgacgtcggg accggcaacc gccgcttcga cgtcgcgcgg gtcgagcacg
      601 tccgccgtca cctgaaagag cttgcgcagg gtgacctgat cgtgctcgcc accgctcacg
      661 ggtggaatga aggcgagttc gtcaccatcc tggagaaggt gatcgccgcg gacgtaggtc
      721 tcgttgacca tcagcatgat cgagcgttcg agaccagcca gccggggtac gcgcgcggcg
      781 atctgggcga aggcgtcctt cgtcgtcgac ccgagcggga gttccatcgt ctccccggcg
      841 gtgccgagca tctcgcgcat catcgcaaag taatgaacct gaatcttgat cgtggttgtg
      901 gatgtcggca tggtcatctt tgggtgaatc agggctgctg ttcgatgaag ttgccgagga
      961 cggcgcgaag ttccatcgcc gtcttccggt tgttgttgcg cagggtccag aagatcttgg
     1021 cgatctcgat cttgcccgct tcctcgaagt cggtggcgta cttcgcgagc acatcctcgc
     1081 tctggaggag cgtgctcagc gtcgtcacga cattgtattc aagatcatcg accgtaaagt
     1141 tcttcgcgtc tgtcgtcatg agtcctcctc cggcatccca gggtgcttgg tcgcagtagt
     1201 ccgcgtcctg gcatcgcgtg cagatttcgt gctgctttcc cgcatcctac caagttccgt
     1261 ctcgcgaacg tgttgaaaga agcttagcgc acctgacggg acgatcgggc cagaccgcgg
     1321 cggacggaag ggcatccgcg gcgcggtgcc tagttggagg cgttcccctc gccatggatc
     1381 agctccgcga agagtggtgt gagcagccgg acgagtacgt caggatcggc gttggcgagc
     1441 gcgggaagcc ccatcatctg ccgcattacc tggaatccgg cgaccagcga gagcacgagc
     1501 gcggcccgtt gtggcgccag gtcgccggcg agtgccccgg tcatcacgcg ttggtggtgc
     1561 ttctcgatct gctcgcggcc gatttccgcc gctcgttcgc tcgatgcgga gtagagcatg
     1621 atcaggaagc catcgagcgg ggtcgtgccg ctcgtggtga tgccgacgag cgcgcgggcg
     1681 aagtgctcgg cgcggtcggg cgccgcgagg ttctccgcgg caaggatcac cgggtcctcc
     1741 atcgcgttgg cgatcacctc ggcaaagaga ttctccttcg agccgaaata gcggttgacg
     1801 agcatcgccg tcacgccggc gtcccgggcg atctcgcgga cgccggcgcc gtcatagccc
     1861 acgcgggcga aggctcgccg ggcggaagcg aggatcgccg cgcgcgtggc ggcggcattt
     1921 cgcgatctgg tgggagttgt cttctccatg agcgtacctc accctgtaac tatacacccg
     1981 tagacatgcc tttctctcta tagtatacat gtgtagacat atcgcgcact tgccctggac
     2041 gccactggtg acagggcatt gcgatcgcaa gcatatggac gcacgggaga aaatcagatg
     2101 gttgaaacgg tccttgtcac aggaggaacc ggctttgtcg gcggctggtg tattgccgga
     2161 ttgctcgagc gcggctatgc cgtccgcacg acggtccgca acctagcgaa ggagccggcg
     2221 gtcaggtccg ccgtcggcgc cgtg
//
LOCUS       DASTMD010000822         1931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_790360, whole genome shotgun sequence.
ACCESSION   DASTMD010000822 DASTMD010000000
VERSION     DASTMD010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790360"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFQ54_12215"
     CDS             <1..515
                     /locus_tag="VFQ54_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165800.1"
                     /translation="SLEENIAIGDPAFTWTDPEPGLARARAVADRVGIGAIAGSLPRG
                     YETELTGLFNDTTDLSGGQWQLVTFARGLIRDDAALAILDEPSSALDPIREREQIDHI
                     RTFARAERRSVLLISHRLSTVRWADRIAVLEGGRIVESGSHDDLVTAGGVYADLFRMQ
                     ASRYRDDAMI"
     gene            726..1664
                     /locus_tag="VFQ54_12220"
     CDS             726..1664
                     /locus_tag="VFQ54_12220"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEU0165801.1"
                     /translation="MNEILNATVPVFALILVGYVCGARGILGAGAVDALNRFVMYLAL
                     PALLFQAMAASKLSDLANPGLLLAFGGGVIVTFAISFAFDMRRYGHLTTASIDGLAAS
                     YPNVGFMGAPLCLIVFGEKSLVPVALAAFVTICLLMTVSIVIIELDRSENSIALWPTV
                     RPILWNLAKNPLVFAPLLGLAVSALGLNVPSAFDRTFNLLGDASSPCALVAIGLFLAE
                     QQVLGDRITVGRIVGLKLLVQPVVTAFIVLVLFPTTAIWSHAIILLSALPVGTGPFML
                     AKMYDQEPAVASRSILLSTIGSLVTISALIVLFEHA"
BASE COUNT          285 a          647 c          609 g          390 t
ORIGIN      
        1 tttctctgga ggagaatatc gcgatcggcg atccggcatt cacgtggacc gatcccgagc
       61 cgggtctcgc gagggcgcgt gccgtcgccg accgcgtcgg gatcggggcg attgcgggga
      121 gtctgccacg tggttacgag accgagctga ctggcctctt caatgacacg accgatctct
      181 ccggtgggca gtggcagctg gtaacgtttg cccgcgggct gatccgtgac gacgcggcgc
      241 ttgcgatact cgacgagccc tcgtccgcac tcgacccgat ccgggagcgc gagcagatcg
      301 accacatccg cacctttgcg cgggccgagc gacgatcggt gttgctgatc agccaccggc
      361 tttcgacggt ccgctgggcg gatcggatcg cggttctgga gggcggtcgc atcgtcgaat
      421 ccggctccca cgacgatttg gtcactgcgg gcggcgtcta cgccgatctc ttccggatgc
      481 aggcgagccg gtatcgtgac gatgcgatga tctgatcgga gaacgagcgc gtccatcgcg
      541 attcgcatcg ggccgatccg gcggcttcat ggcatactcg gggaacctct ccggacagtg
      601 acgttccgga tcgacggccc cgggcgccac cgccgctgcc tcctgccaat cgccatcgca
      661 cgatcccctc acccctctat ccatcgagcg gtgccggaat cgcgtcctct tccagaaagt
      721 cccgcgtgaa cgagatcctc aatgcgacgg tgccggtctt cgcgctgatc ctggtcggct
      781 acgtctgtgg tgcgcgcggg attcttggcg ccggcgccgt cgatgcactg aaccgtttcg
      841 tgatgtacct cgccctcccc gccctgctct tccaggcgat ggcggcctcg aagctctccg
      901 atctcgcgaa tcccgggctg ctgctcgcct tcggtggcgg tgtcatcgtc accttcgcga
      961 tctcgttcgc cttcgacatg cggcgatacg gccatctgac gacggcgagc atcgacggac
     1021 tggcggcctc gtacccgaac gttggcttca tgggtgcgcc gttatgtctc atcgtcttcg
     1081 gggagaagag tctcgtcccg gtcgccctgg cggcgttcgt cacgatctgc ctcctgatga
     1141 cggtctcgat cgtgatcatc gagctcgatc gctcggaaaa ttccatcgca ctctggccga
     1201 cggtccggcc catcctctgg aatctggcga agaatccgct cgtcttcgcg ccgctcctcg
     1261 ggctcgccgt cagcgcgctt ggattgaacg tcccgtcggc cttcgaccgg acgttcaatc
     1321 ttctgggaga tgcctcgagc ccgtgcgcac tggtcgcgat cgggctcttc ctcgcggaac
     1381 agcaggtatt gggcgaccgg atcacggtgg ggaggatcgt cgggctgaag ctgctcgtgc
     1441 agccggtggt caccgccttc atcgtgctcg tcctcttccc gacgacggcg atctggtccc
     1501 acgcgatcat cctgctcagc gcgctgccgg tcggtaccgg gccgttcatg ctcgcgaaga
     1561 tgtatgacca ggaaccggcg gtcgcgtcgc gttcgatcct gctctcgacg atcggctcgc
     1621 tggtcacgat ctcggcgctg atcgtcttgt tcgagcacgc gtaactgagg cgtcaatgct
     1681 ggatcgtccc actgatgcca tcgttggatg ccccactcca ccatagccac catcctgagc
     1741 atcgcgaagg agtcctgcgc gaatcgctcc cggcgcagct ggcttcagct tcaaccccag
     1801 cgttcgcgga acggtgtttt ccggagacgg atcggcttcg ccgacccgcg cgcggccaca
     1861 agggccgcgg ggacgggaaa gcggacgcct cgttctccag acgtcatgaa tgggaggccc
     1921 cgggtgatcc c
//
LOCUS       DASTMD010000823         1675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1864493, whole genome shotgun sequence.
ACCESSION   DASTMD010000823 DASTMD010000000
VERSION     DASTMD010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864493"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFQ54_12225"
     CDS             <1..398
                     /locus_tag="VFQ54_12225"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEU0165802.1"
                     /translation="SGITRGTTAAHIARATLEAIAFQGRDVLDAMQSDAGIELGELHV
                     DGGASQNDLLMQIQADILGVPVVRPKVVETTVRGAAFLAGLATGIWNDREELRARLAI
                     DRRFEPAIPASERDERYAIWQERVGRTRT"
     gene            508..1101
                     /locus_tag="VFQ54_12230"
     CDS             508..1101
                     /locus_tag="VFQ54_12230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU0165803.1"
                     /translation="MSDATGPIKLGIIIASTRPGRVGLPVGQWVAQAAAEHGGFEIDV
                     IDHAEVNLPLMDEPAHPRLRQYTHDHTIAWSERIDALDAFVVVMPEYNYGFTAPLKNA
                     LDYLSQEWQYKAIGIVSYGGLSGGLRAVQMLKPVLLALKLSPINEAVAITAVSSHLDD
                     EGVFEPTESLSGQARTMFAEIQRWVPALRPLRAPALA"
BASE COUNT          287 a          508 c          591 g          289 t
ORIGIN      
        1 tttccggcat cacacggggc acgaccgccg cccacatcgc gcgcgccacg ctggaggcga
       61 tcgcgttcca ggggagagat gtcctcgacg ccatgcaatc cgacgccggg atcgagctcg
      121 gcgagcttca tgtcgacgga ggagcctcgc agaacgatct gctcatgcag atccaggcgg
      181 acatcctcgg cgtgccggtt gtgcgaccga aggtcgtcga gacgactgtg cgcggcgcgg
      241 ccttcctcgc gggattggcg accgggatct ggaacgaccg cgaggaactc agggcccgcc
      301 tcgcgatcga ccgtcgcttc gaaccggcga tcccggcgtc cgagcgcgat gagcggtacg
      361 cgatctggca ggagcgcgtt ggccgaaccc ggacctgacc tgcagatgtg cgctggaaaa
      421 cttacgattg gtaagtataa tttcggcaga caacggtgaa ccgggcgcgt cgacgcccgg
      481 ccttttcatg atcgagggag cgaatcaatg tcagacgcga cggggccgat caagctcggc
      541 atcatcatcg cgagcacgcg tccggggcgc gtcggtctgc cggttgggca atgggtggcc
      601 caggctgcgg cggagcatgg cgggttcgag atcgatgtga tcgatcatgc cgaggtcaat
      661 ctcccgctga tggacgaacc cgcgcatcct cgcctgcggc agtacaccca cgaccacacg
      721 attgcctgga gcgagcggat agacgcgctc gacgcgttcg tcgtggtgat gccggagtac
      781 aactacggat tcacggcgcc cttgaagaac gcgctcgact atctctcgca ggaatggcag
      841 tacaaggcga tcggcatcgt cagttacggc ggactttccg ggggcctgcg tgcggtacag
      901 atgctgaagc cggtgttgct ggccctgaag ctgtcaccga tcaacgaagc ggtggcgatc
      961 accgccgtga gcagccacct cgacgacgag ggcgtcttcg agccgacgga atccctgagc
     1021 ggtcaggcgc ggacgatgtt cgccgagatt cagcggtggg ttccggcgct tcggccgctc
     1081 cgcgcacccg cgctcgcctg acgtcatctc gcgctgcgtg gtggttcgcg gcgagatgag
     1141 gacgcagggg gcgtaccgtg cggggcgcgt cgtcccggct ggggagttgg ggaggcggga
     1201 caggccgtcc agatctgcga tctggcagcg gcggggggac gcaaggatgg ggcacgtcgt
     1261 ctcggttggg gagatgggga ggcgggacag gccgtccaga tctgcgatct ggcagcggcg
     1321 gtcccgccct acgaacgacc gcgttatggt cggtcttcct cgtcgtggcg gggcatctgg
     1381 tctgacccat cgcgcgtcgc gcgttgcgat tcgcgctggt ggtgcgtcac cgctgaaggg
     1441 ccacggtgat ctcggagcgg gccaccaacg agcctgccct gcgttctccg aagggtgtgg
     1501 cccacgccgc gaagccgcat atgatcgttg gcttactacg gtgccggccg acaagacacc
     1561 ggtgacagac cactacagcg tcttgttgag ccggtaccat tccttttcga agccgaggtg
     1621 ttcgtagagt gcgagtgccc gatcgttacc gagatagcag tgcaacgtga cctcg
//
LOCUS       DASTMD010000824         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1507410, whole genome shotgun sequence.
ACCESSION   DASTMD010000824 DASTMD010000000
VERSION     DASTMD010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507410"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..884)
                     /locus_tag="VFQ54_12235"
     CDS             complement(6..884)
                     /locus_tag="VFQ54_12235"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165804.1"
                     /translation="MTTYRIAAPAKINLGIEILGKRSDGYHEIRSILAAVDLVDTLTA
                     TPVPGAEATVVTGMDISPEENLILRALNAVDPRSAIRVAIEKRIPMAAGLGGASADAA
                     AALLIANAYRTNPLAKTDLHRLAAGLGSDVPFFLGGPCAHVSGTGTETVAVAPPAGFA
                     VLFTPSIEIPRKTAALYGSLESGDFSDGERIATQRSRLDEGRELEPALLANAFSRALE
                     SLAPPIVDLKRAARDAGAPFVALSGAGPSHYTIMSTEDDANALASRWSRFLPPDVAIH
                     VAAFRATPLQLDVVAR"
     gene            complement(881..1735)
                     /gene="rsmA"
                     /locus_tag="VFQ54_12240"
     CDS             complement(881..1735)
                     /gene="rsmA"
                     /locus_tag="VFQ54_12240"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU0165805.1"
                     /translation="MTHAAASANQRTRSDWERVLRDHDIRPTRSMGQNFLVDPAIVDE
                     IVLVAGVGSGDRVVEIGPGMGILTRALLGAGARVTGVELDRDLATFLRNDLAGVAGFS
                     LVERDARHVDVVDVSENESFAVVANLPYSVATVVIRHFVEAANPPERLTVMVQREVAE
                     RMTADPPGMSLLGLATQLYSDAGIAFIVPPDVFLPPPKVESAVVKLDVKVDLPLDLPD
                     RNRLFELATMAFQRKRKTVANGLSMGLDRAKSEVDDMLRGAGIDAGLRPQALGLEEWI
                     EIAKAFRA"
     gene            1902..2273
                     /locus_tag="VFQ54_12245"
     CDS             1902..2273
                     /locus_tag="VFQ54_12245"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEU0165806.1"
                     /translation="MTIYRTAEEARNADVTYHMTPLEVWERQQYQQDYYPEAFPNDGF
                     IHCTNGTDQLIWVANTFYTAERREIVVLALSVPAITSEVRYDDPEEKFPHIYGPLNPS
                     AVIGKLRVVRAGDGGFLAIEE"
     gene            2310..>2666
                     /locus_tag="VFQ54_12250"
     CDS             2310..>2666
                     /locus_tag="VFQ54_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165807.1"
                     /translation="MFERLGRFAARHRWIVIAIWALLVLVALPTLPGLETHLKVGGFT
                     SDHTEGAKAVDELEQSLGLSPSTLVVVYQSDTLAIGSDEFNREVSASLAKMRAVPGVT
                     GVVLPSMDPTLVAPTKK"
BASE COUNT          503 a          825 c          879 g          459 t
ORIGIN      
        1 gcgattcacc gtgcgacgac gtcgagctgg aggggagtcg cgcggaaggc ggcgacgtgg
       61 atcgcgacgt caggcgggag aaaccgggac catcttgagg cgagcgcgtt cgcgtcatct
      121 tcagttgaca taatcgtgta gtgcgatggg ccagcaccgg agagcgcgac gaagggtgca
      181 ccggcgtcac gcgcggcccg tttcaagtcg acgatcgggg gtgcgagcga ttcgagggcc
      241 cgcgagaacg cgttcgcgag aagggccggt tcgagttccc ggccctcgtc cagtcgcgac
      301 ctctgggtgg cgattcgctc gccgtcactg aagtcccccg attcgaggct gccgtacagt
      361 gccgccgtct tgcgcggaat ctcgatcgag ggagtgaaga gaacggcgaa tccggcgggc
      421 ggagcaaccg cgaccgtctc ggtgcctgtg cccgacacat gggcacaggg gccgccgaga
      481 aagaacggga cgtcgctgcc caatcccgca gcaaggcgat gcagatcggt tttcgcgaga
      541 ggattggtgc gataggcatt ggcgatgagg agagccgctg ccgcatcggc gctggcgcca
      601 ccgagacccg ccgccatcgg aatccgcttt tcgatcgcga cgcgaatagc cgaacgggga
      661 tcgacggcat tcagggcgcg gaggatcaga ttctcttcgg gggagatgtc catacctgtc
      721 acgaccgtag cttcggctcc gggaacgggg gtcgccgtca acgtgtcgac gagatcgacc
      781 gcggcaagga tcgagcgaat ctcgtgatag ccgtccgatc gctttcccag gatctcgatc
      841 ccgagattga tcttcgcagg ggccgcgatg cgatacgtgg tcacgcgcga aacgccttcg
      901 caatctcgat ccactcttcg agacccaaag cctgggggcg gagcccggcg tcgattccag
      961 cgccgcgcag catgtcatct acttcggact tggctcgatc gagacccatc gagagaccgt
     1021 tggcgaccgt cttccgcttg cgctggaatg ccatcgtcgc cagttcgaag aggcgattcc
     1081 gatccggcag atcgagcgga aggtcaacct tgacgtccag cttcacgacg gcggattcga
     1141 ccttgggcgg cgggaggaag acgtccggcg gcacgatgaa ggcgatcccg gcgtcgctgt
     1201 agagctgcgt cgccagaccc agcagggaca tgccgggtgg atcggcggtc atccgctcgg
     1261 caacctcccg ctgcaccatc accgtcagtc gctcgggagg gttcgccgcc tcgacgaagt
     1321 gacggatgac gaccgtcgcc acgctgtatg gcagattcgc gacgacggcg aatgactcgt
     1381 tctcgctgac gtcgacgacg tcgacgtgcc tggcatcgcg ctcgacgagt gagaatccag
     1441 cgacgcccgc gagatcgttg cgcaggaagg tcgcaagatc gcgatcgagc tcgacgccgg
     1501 tcacgcgtgc cccggctccc agcagcgccc gggtgagaat tcccatcccg gggccgatct
     1561 cgaccaccct gtcgccagaa ccgacgcctg cgacgaggac gatctcgtcg acgatcgcgg
     1621 gatcgacgag aaagttttga cccatcgatc gtgtcggacg gatatcgtga tcgcggagga
     1681 cacgctccca gtcggaccgg gtacgttggt tggcggaagc tgcggcatga gtcatggggt
     1741 cggtagtctc gcttgggcgg agtgtcggta tcgatcgtcc gctccactat gccatacgtc
     1801 gatcgatctc gatgaaaagg cgcccgggta caattcgcgc tgtacgattc ccagcggatc
     1861 cttcgatcac gcttcaaccg gatgaaatgg agaacccgtt catgaccatc tatcgcactg
     1921 ccgaagaggc tcggaatgcc gatgtcacgt accacatgac cccgctggag gtctgggagc
     1981 gccagcagta tcagcaggac tattaccccg aggccttccc gaatgacgga ttcatccact
     2041 gcacgaacgg aaccgaccaa ctgatctggg tcgcgaacac cttctacacg gcagagcgtc
     2101 gagagatcgt cgtgctcgcg ctgagcgtgc cggcgatcac gtccgaagtc cgctacgacg
     2161 acccggagga gaagttcccg catatctatg gaccgctcaa tcccagcgcg gtgatcggga
     2221 agctccgggt cgtgcgcgcc ggtgacggcg gattcctcgc catcgaagag tagtggcgac
     2281 gctgtctgga gtctgtctga aagccccgga tgtttgagcg actcggccga tttgccgccc
     2341 ggcaccgctg gatcgtgatc gcgatctggg cgctgctggt cctcgtcgcc ctgccgacgc
     2401 tgcccgggct cgaaacgcac ctcaaggtcg ggggattcac ctccgaccac accgaagggg
     2461 cgaaggccgt cgacgagttg gagcagtcgc tcggactcag cccatcgaca ttggtcgtcg
     2521 tctatcagag cgatacgctc gccatcggca gcgacgaatt caatcgggag gtctccgcct
     2581 cgctcgcgaa gatgagagcg gtgccgggcg tcaccggcgt cgttctcccg tcgatggatc
     2641 cgaccctggt cgcgccgacc aagaag
//
LOCUS       DASTMD010000825         1939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_614540, whole genome shotgun sequence.
ACCESSION   DASTMD010000825 DASTMD010000000
VERSION     DASTMD010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614540"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VFQ54_12255"
     CDS             <1..1022
                     /locus_tag="VFQ54_12255"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU0165808.1"
                     /translation="IAPMNAIPFTDPSPAKIDAVRERLLTWWQANRRDLPWRHTRDPY
                     RILVSEVMLQQTQVDRVIPYYEQWLAAFPTVQALAEAPTAEVIRLWAGLGYNRRAVNL
                     QRTAQAVVERGGDFPGTVEELRLLPGIGPYTSGAIACFAFEQDVPFIDTNMRRVLHRV
                     FAGVDVPEPTLSDREITAIASDVIPPGDGWNWNQALIEFGALQCTARKPLCIVCPLRD
                     ACVAAPTIQTAIATLPKGARKKKEGPFKESNRYFRGRVIDILRDRDRAHRGVPLAEIG
                     PEVRPEFQDADLPWLFEVVRGLERDGLAMVAEDEPSYDASSLAAEGVDDVDGPDIGNI
                     RVRLP"
     gene            1099..1728
                     /locus_tag="VFQ54_12260"
     CDS             1099..1728
                     /locus_tag="VFQ54_12260"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEU0165809.1"
                     /translation="MYLPAHFEMTDPEAIRAMIAEHPLGTLVTLGRDGIAANHIPFLL
                     EERGEHGTLVAHVARNNPLWTDRGPYVEALTIFQGPTAYISPNWYPTKQESHEVVPTY
                     NYAVVHVYGELIVHDDPKWVRGAIGKLTKAMEASQSKPWKMADAPQDYLATMVANIVG
                     IEIPISRFSAKWKASQNRTPADLAGVISGLREMSDPEDAAMAAVMDTFR"
BASE COUNT          345 a          616 c          629 g          349 t
ORIGIN      
        1 caatcgctcc catgaatgcg attcccttta ccgatccatc acccgcgaag atcgacgcgg
       61 tgcgcgaacg gctcctcacc tggtggcagg cgaatcgccg cgatctcccc tggagacaca
      121 cgcgcgatcc ctaccggatt ctcgtctcgg aggtcatgct ccagcagacg caggtcgatc
      181 gcgtcatccc ctattacgag cagtggctgg cagcgttccc gactgtccag gcgctggccg
      241 aggcaccgac cgccgaggtg attcggctct gggcggggct cggctataac cggcgcgcgg
      301 tcaatctcca gcgaacggcg caagccgtcg tcgagcgcgg cggcgacttt cccgggacag
      361 tcgaggagtt gcgactgttg ccgggcatcg gtccctacac atctggcgcg atcgcctgct
      421 ttgcgttcga gcaggatgtc ccgttcatcg atacgaatat gcggcgcgtg ctgcatcgcg
      481 tattcgccgg tgtggacgtg ccggaaccga cgctcagcga tcgtgagatc accgcgatcg
      541 cttcggatgt cattccgccc ggtgacggct ggaactggaa ccaggcgctg atcgagtttg
      601 gcgccctgca gtgcacggcg cgcaagccgc tctgcatcgt ctgtccgctg cgggatgcct
      661 gtgtcgccgc gcccacgatc cagaccgcga tcgcaacact gccgaagggt gcacggaaga
      721 agaaggaagg gccgttcaag gagtcgaatc gctacttccg ggggcgcgtc atcgacatcc
      781 ttcgcgaccg cgatcgcgcg catcggggcg ttccgctcgc cgagatcgga cccgaagtgc
      841 gtcctgagtt ccaggacgcc gatctgccgt ggctcttcga agtcgtgcgg gggctggaac
      901 gcgacgggct ggcgatggtc gccgaggatg agccgtcgta tgatgcctct tcattggctg
      961 ccgagggcgt cgatgacgtc gacggtccgg atatcggaaa catccgcgtg cgacttccgt
     1021 agtctccggc tcctgggttc ccgccgccgg tagatccgcc caggaatcgt cacgcttaac
     1081 tcacccacga ggctcaccat gtatctcccc gctcatttcg agatgactga tccggaggcg
     1141 atccgcgcca tgatcgccga gcatccgctc gggaccttgg tgacgcttgg tcgggacggg
     1201 attgctgcga atcacatccc gttccttctg gaagagcgtg gagaacatgg cacgctggta
     1261 gcgcacgtcg cgcggaacaa tccgttgtgg acggaccgcg ggccgtatgt cgaggcgctg
     1321 acgatcttcc agggaccgac ggcatacatc tcgccgaact ggtacccgac gaagcaggag
     1381 agtcacgagg tcgttccgac atacaactac gcggtcgtcc atgtctacgg cgagttgatc
     1441 gttcacgacg atccgaagtg ggtacgcggc gcgatcggca agctgacgaa ggcgatggag
     1501 gcatcgcagt cgaagccgtg gaagatggcc gacgcgccgc aggactatct ggcgacgatg
     1561 gtcgcgaaca tcgtggggat cgagattccg atcagccgct tttcggcgaa atggaaagcg
     1621 agccagaatc gaactccggc ggacctcgcc ggtgtgatct ccggtctgcg ggagatgagt
     1681 gatcccgagg atgccgcgat ggcggcggtg atggatacct tccgctagaa cacgcccatc
     1741 gtgccgccga ggttgctggt ggggacgtcc cgcggattgg caccaggcgg gacattccaa
     1801 gcttggcctg agctcggtcg aacgggtcca ccgaccggac cttccgcccc aaagacgctc
     1861 cgagccccgg tcgccggact ccgtagtccc gccccacgca cgaccgcgtg caatgcggaa
     1921 ctccgtgcca tgccttccg
//
LOCUS       DASTMD010000826         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_255105, whole genome shotgun sequence.
ACCESSION   DASTMD010000826 DASTMD010000000
VERSION     DASTMD010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255105"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1268
                     /gene="modA"
                     /locus_tag="VFQ54_12265"
     CDS             315..1268
                     /gene="modA"
                     /locus_tag="VFQ54_12265"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0165810.1"
                     /translation="MKQRSSQGSIAARLRHAMRALMLVPMLMLGFLMAPGAAGIAAAA
                     TTIDCPPATPAAASSASASASPTLATPAAATEFPTDGGSLTVYAAASLTDAFGKIGDQ
                     ITAAHPNVKISFQTGGSQALVTQLQQGAKGDVLATANTSTMASAVKSNLIDGSPITFT
                     GNRLVIVTPTDNPASITSFDDLAKSGTKLVLAAPDVPAGNYAVKAICQHQQDVSGFAG
                     EIGKNVVSEEEDVRTVLSKVQTGEADAGIVYYSDAVASELSGTKLNVIEFPATVPTYA
                     SYPIAPIAGGNTDLANAFIAYVLGPDGQKVLKDYGFETLAS"
     gene            1353..>2187
                     /locus_tag="VFQ54_12270"
     CDS             1353..>2187
                     /locus_tag="VFQ54_12270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165811.1"
                     /translation="MTQRTTPAGKRSMTGGGAFSSVLWALTLVGLALLTLPTASLVWR
                     AYEARDSINNPHARDVLNDALTLSVTTTLLALALIVIFGTPVAYVLARAKFRGARLVD
                     VLIDLPIILPPSVAGIALLAAFGRRGIFGTWLNDWGITIGFTTTAVVIAQIFVAAPFY
                     IRTARAGFLRVDRSLEEAAADLGATPMRTFFRVTLPLCRGSLLAGAILAWARALGEFG
                     ATIMFAGNLQGVTQTLPLAIYERFGAGDLNSALVLSVVLLATSLVFLIVVRILGEESS
                     PR"
BASE COUNT          354 a          755 c          689 g          389 t
ORIGIN      
        1 atccctcgac aggctcggga ggacgtaccg gggggagggg gttgcatgcc tggcacgcgg
       61 tatcgaggcg gtccctcgac aagctcggga ggacgtatcg ggggacgggg ttgcatgcat
      121 ggcgggcggg tggtgaccag ccggccgctc gacaggcgcg ggggacggga gatggacgcg
      181 gcggaagcta cacgtacttg tgatgccaga aacggatagg cttgccggga aggtggttgc
      241 cgggtaggat gcgattgtag tcaagtcatg actaagaggt ttttgattag ccaatgttcc
      301 agggagagtt gtccatgaag cagcgttcct cacaggggtc gattgcggcc cgccttcgtc
      361 acgcgatgcg cgcgctgatg ctcgtaccga tgctgatgct cgggttcctc atggccccgg
      421 gagcagccgg aatcgccgcc gcggcgacca ccatcgattg cccgccggcg accccggccg
      481 cagcatcttc cgcctcggcc agtgcctccc ccacgctcgc gaccccggca gctgccacgg
      541 agttcccgac cgacggtgga tcgctcacgg tctacgccgc ggcctccctc acggatgcct
      601 tcggcaagat cggcgaccag atcaccgctg cccacccgaa cgtgaagatc tccttccaga
      661 ccggtggctc gcaggcgctc gtcacccagc tccagcaggg cgcaaagggc gatgtcctcg
      721 ccacggccaa cacctcgaca atggccagcg ccgtcaagag caacctgatc gatggcagcc
      781 cgatcacctt caccggtaac cgcctcgtca tcgtgactcc gaccgacaac ccggccagca
      841 tcacctcgtt cgacgacctc gcgaagagcg gcaccaagct cgtcctcgcg gcgccggacg
      901 ttccggccgg caactacgcg gtcaaggcga tctgccagca ccagcaggat gtctccggtt
      961 tcgccggcga gatcggcaag aacgtcgtct cggaagaaga ggatgttcgc acggttctct
     1021 ccaaggtcca gaccggtgag gccgatgctg gtatcgtgta ctacagcgat gccgtcgcct
     1081 ccgagctttc cggcaccaag ctgaacgtga tcgaattccc ggcgacggtc ccgacctacg
     1141 ccagctatcc gatcgccccg atcgccggtg gcaacacgga tctcgcgaac gcgttcatcg
     1201 cgtacgtcct cggtccggac ggccagaagg tcctcaagga ctacggtttc gagaccctcg
     1261 cctcctaggc gtctctccac gaaaccggag gtcgcttctg cggcctccgg ttttgttctt
     1321 tgccagcgca ggacctacag gaacggacgt cgatgacaca gcgcaccacc ccggccggca
     1381 aacgatcgat gaccggcggc ggtgcgttct cgtcggtgct ctgggctctg accctcgtcg
     1441 gtctcgcact cctcacgctg ccgaccgcct ccctggtctg gcgtgcctac gaggcgcgcg
     1501 attcgatcaa caacccccac gcgcgcgatg tcctcaacga cgcactcacc ctcagcgtca
     1561 cgacgacact gctcgccctc gccctgatcg tgatcttcgg gacaccagtt gcgtatgtcc
     1621 tcgcgcgggc gaagtttcgc ggcgcgcggc tcgtggacgt gctgatcgat ctcccgatca
     1681 ttctcccgcc atccgtcgct ggaatcgcgt tgctggcggc attcggccgg cgcgggattt
     1741 tcggcacgtg gctgaacgac tggggaatca cgatcgggtt cacgacgacc gccgtcgtga
     1801 tcgcccagat cttcgtcgcc gcacccttct acatccggac cgcgcgggcg ggattcctcc
     1861 gtgtcgatcg ctcgctcgag gaggccgccg ccgacctcgg ggcgacaccg atgcgcacct
     1921 tcttccgcgt gacgctgccg ctctgccggg ggagcctgct ggccggggcg atcctcgcct
     1981 gggcgcgggc gctgggagag ttcggggcga cgatcatgtt cgccgggaat ctccagggcg
     2041 tcacccagac gctgccgctg gcgatctacg agcgcttcgg cgcgggcgat ctcaactcgg
     2101 cgctggtgct ctccgtcgtc ctcctcgcga catccctcgt cttcctgatc gtcgtccgga
     2161 tactcggtga ggaaagctcg ccgcgct
//
LOCUS       DASTMD010000827         1882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_164553, whole genome shotgun sequence.
ACCESSION   DASTMD010000827 DASTMD010000000
VERSION     DASTMD010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164553"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..>1882
                     /locus_tag="VFQ54_12275"
     CDS             132..>1882
                     /locus_tag="VFQ54_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0165812.1"
                     /translation="MATPINPERLINELPSIASPRISPDGSQIAYVRRTVNVESGKDE
                     TAIWLAGIDGSNRRQLTFASARNGNPAWSPDGSSLAFVTSRDDRTALAILDLAAGGEA
                     RIVTTQDQAISGIAWSPDGGSIAFASPVDPENPNEPRRDPKAPAAVRVVKRIDYKQDG
                     LGYLNDVRNQIFVVDVTSGTTSGEKRQLTHDTIDHVGPAWSPDGSKLAISLPHHNTLL
                     AGLGIIDVASAGFTRFGPDNAAIGNIRWTPDGAGILHTEGFNIQPSQAWFLYDVASGE
                     AKQLTEEIEFSAGGEESGTPWLDATHAAFLGGSRGRTGVWTIDIASGEITEIAIWNWL
                     GRQADFNAGTETAVIAMTGLDGVVGLTRIDLATGAETLLFNEATGFFSEFKPSQWELV
                     TIERNGYTIEGWLHKPADFDESKKYPVVLDVHGGPHGFYGYTLDELTETLATNDILVI
                     KSNPRGSGSYGPAFGKAVIGDWGGEDWKDLQGILDKVLERPYADAERTGIYGYSYGGY
                     MTSWAIGQTTRFKAAVCGAPCYDFESFFGTSDVGHIFGSQGYKQLPWENTAITLERSP
                     SSHIQNATTPTLVIHGEA"
BASE COUNT          369 a          643 c          574 g          296 t
ORIGIN      
        1 ggctcgacgt cctcacccgc accctcgcct ggtacaagcg attcctcggc gacccggcgt
       61 gacgcggcga gcgcagcccg atacccgatc gaagacccga gtattcagag aaccaaccca
      121 gggagaacct tatggcaaca ccaatcaatc ctgaacgcct gatcaacgaa ctgccgtcga
      181 tcgccagccc gcgaatctcg ccggacggtt cgcagatcgc ctacgtccgc aggaccgtca
      241 acgtcgaatc cgggaaggac gagaccgcga tctggctcgc cggcatcgac gggtccaatc
      301 gtcgccagct caccttcgcc agtgcccgga acgggaaccc ggcctggtct cccgacggct
      361 cgtcgctcgc cttcgtcacc tcgcgggatg acaggacggc actcgcgatc ctcgatctcg
      421 ccgccggtgg cgaagcgagg atcgtgacca cccaggacca ggcgatcagc gggatcgcct
      481 ggtcgccgga cggcggatcg atcgccttcg catctcccgt cgatccggag aatccgaacg
      541 aaccgcggcg cgatccgaaa gcccccgccg cggttcgcgt cgtcaagcgg atcgactaca
      601 agcaggacgg cctcggctac ctgaacgatg tccgcaacca gatcttcgtc gtcgatgtca
      661 ccagcggcac gaccagcggt gagaagcgcc agctcactca tgacacaatc gaccacgtcg
      721 gcccagcctg gtcgccggac ggctcgaaac tcgccatctc gttgccgcat cacaacaccc
      781 tgctcgctgg cctcggcatc atcgacgtcg cgtccgccgg cttcacgcgc ttcggtcccg
      841 acaacgccgc gatcggcaac atccgctgga cgccggacgg ggcgggcatc cttcacaccg
      901 aagggttcaa catccagcca tcacaggcat ggtttctcta cgatgtcgca tcgggcgagg
      961 caaagcagct tacggaggag attgaattct ccgcgggcgg cgaggagagc ggcacgccgt
     1021 ggctcgatgc gacccacgcc gccttcctcg gcggatcgcg cggtcgcacc ggggtctgga
     1081 cgatcgacat tgcgagcggc gaaatcacgg agatcgcgat ctggaactgg ctgggtcgcc
     1141 aggccgactt caatgccggc acggagacag cggtcatcgc gatgaccggt ctcgacggcg
     1201 tcgtcggtct cacacgcatc gacctcgcga ctggcgccga gacacttctc ttcaacgagg
     1261 cgaccggctt cttctcggag ttcaagccgt cgcagtggga actcgtcacg atcgagcgca
     1321 acggctacac gatcgagggc tggctccaca agccggccga tttcgacgaa tcgaagaagt
     1381 acccggtcgt gctcgacgtt cacggcggcc cgcacggctt ctatggatac acgctcgacg
     1441 aactgaccga aacgctggcg accaacgata tcctcgtcat caagtcgaac ccgcgtggct
     1501 cgggatccta cggaccggcc ttcggcaagg cggtgatcgg cgactggggc ggggaggact
     1561 ggaaggatct ccagggcatc ctcgacaagg tcctcgaacg cccctacgcg gacgccgagc
     1621 gaacgggaat ctatggctat tcgtatggcg gctacatgac ctcgtgggcg atcggccaga
     1681 cgacgcgctt caaggcggcg gtctgcggcg caccgtgcta tgacttcgaa tcgttcttcg
     1741 gaacgagcga cgtcgggcat atcttcggtt cgcagggcta caagcagctg ccgtgggaga
     1801 acacggcgat cacgctcgag cgctccccgt cctcccacat ccagaacgcg acgacaccga
     1861 cgctggtgat ccacggtgag gc
//
LOCUS       DASTMD010000828         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2134443, whole genome shotgun sequence.
ACCESSION   DASTMD010000828 DASTMD010000000
VERSION     DASTMD010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134443"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFQ54_12280"
     CDS             <1..324
                     /locus_tag="VFQ54_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165813.1"
                     /translation="GYAEPESGSCGRLRYHPIPPDSSHDHIKIVIRPTTKQWGEDIEE
                     FALERVAKKVGDEIPDMRQKFQKILARDGIVGNKPGEGIIERHPVGKMPGPTLHQPTE
                     SGIPE"
     gene            664..1671
                     /locus_tag="VFQ54_12285"
     CDS             664..1671
                     /locus_tag="VFQ54_12285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEU0165814.1"
                     /translation="MTNLPLSILDRSHLSAGSDQSAALQRTIERAQHAEQLGFKRFWV
                     SEHHGVPGVAGAAPAVFIAALAARTQRIRLGSGGVMIPNHQPLVIAEQFGTLALLHPG
                     RIDLGIGRSLGFTRPVRRALRRDTYDLDDLRNDLFELRAYLEGTAEITAYPGRDARRA
                     ADLDLFLLASSSSAGIAAQAGLPLVLGGQTLHDEKSIAVVEGYRDAFRPSQFALEPSI
                     ILNVTALVADTEADARDLAISEAWAHVSSKIQGEFPPLEDPARIRTLELTSRQQSRLE
                     TIAAGTLTGTVDRVATTIAQLAKQTDAREVLLSGSFFDRDAALHSDALLARAFGRAPE
                     L"
     gene            1882..2862
                     /locus_tag="VFQ54_12290"
     CDS             1882..2862
                     /locus_tag="VFQ54_12290"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0165815.1"
                     /translation="MITNGSPAGTSRPATSDILRSRPIRLAIFGDAENARRLRDLPDV
                     DIRWLVKRDRADGSIAGALADRDVDAVLIDLPIAPWYDIAVDAARAGKHLFLAAPLAI
                     TVDDARALVDVCADAGVSIVIDNTVRFTPEYRKIAQIIAAGELGQIATIRTNRVIAPP
                     ETGDIIDHALRHDLDALRNIFGDIRRLHAHAAMSRLGRPRQEFAAASIRFANGAIAHI
                     EGRLSPTTPRVAIEIAGQNGILSFDSDAASTLTIERTTATPLRTLARPAVATPEQISL
                     QNFLASLRAEEAEITTGAEAIETLRLVGKLRESIATGAPVAASCTEESPV"
BASE COUNT          518 a         1009 c          853 g          493 t
ORIGIN      
        1 ggttatgccg aacctgagag cggctcgtgc gggcgtttgc ggtaccatcc catcccacca
       61 gactcgagcc atgaccacat caaaatcgtc atcaggccga cgacgaagca gtggggagag
      121 gatatcgaag agttcgccct cgagcgtgtt gccaagaagg tcggcgatga aatccctgat
      181 atgcgtcaga aattccagaa gatcctcgcg cgcgatggaa tcgtcggcaa caagccgggc
      241 gagggcatca tcgagcgaca tcccgtcggc aagatgcccg ggccaacgtt gcatcagcca
      301 acggaatcgg ggatcccgga gtgaaacgcc ctgaaggtcg ggcgtaacca gaattgcggc
      361 gaggaggctc gcaacaggat cgtgtgcatt tggatcggcg cccacgggtg gccctttcgg
      421 agttacatcc atcaaacaag accgcaggga cccgaaggca aactccgccc cagtcccacg
      481 acacgcgcgg aagccgggat ctcgtctgca acacagattg aacgtggact ctgtcaaatc
      541 gacgatttga cttccatatg cgtccaggca ctactaatgt agggaatgcg aaggcgatgg
      601 acctccgtat tcttactgat ttcttgcgaa cagaatccgc atctcactga agcgacttcc
      661 tgaatgacca atctcccgct ttccatcctc gatcgctccc atctctccgc cgggagtgat
      721 cagtccgccg cactccagcg cacgatcgaa cgcgcccaac acgcggagca gctcggcttc
      781 aagcggttct gggtctcgga gcatcacggg gtgccgggag tcgccggtgc cgcgccggcg
      841 gtcttcatcg ccgcgcttgc ggcgcggacg cagcgcatcc gcctcggatc cggcggcgtg
      901 atgatcccga accaccagcc gctcgtcatc gccgagcaat tcgggacgct cgcgctcctt
      961 catcccggcc ggatcgatct cggcatcggg cgctcgctcg gcttcacccg cccggtgcga
     1021 cgcgcgctcc gtcgcgacac ctacgatctc gatgacctgc gcaacgacct cttcgagctt
     1081 cgcgcctatc tcgagggaac cgccgagatc accgcctatc ccggccgcga cgcccgtcgc
     1141 gctgctgatc tcgacctctt cctcctcgcc tcgtcgtcga gcgccgggat cgcggcccag
     1201 gccgggctcc cgctcgtgct cggcgggcag acgctgcacg acgagaagag catcgccgtc
     1261 gtcgagggct atcgcgacgc atttcgtccg tcgcaattcg ccctggagcc gtccatcatc
     1321 ctgaacgtca ccgcgctcgt cgcggacaca gaggcggatg cgcgcgatct cgcgatctcc
     1381 gaggcgtggg cacacgtctc ctcgaagatc cagggggaat tccccccgct cgaggatccg
     1441 gcgcgcattc gcaccctcga gctgacgtcc cggcagcaat cgcgcctgga gacgatcgcc
     1501 gccggtacac tgaccggcac tgtcgatcgg gtcgcgacaa cgatcgccca gctcgcgaag
     1561 cagacagatg cccgggaagt cctgctttcc ggcagcttct tcgatcggga tgccgccctg
     1621 cattccgacg cgttgctcgc ccgggcgttc ggacgcgcac ccgaactgtg accgcgattt
     1681 gggcaattca tccaattctt gacaggcggc aacaattccc cccattcggg ccgcccgcaa
     1741 ttgatgaagc gatgttcggg accgcttgcg gcgatacccc tcgcccccgt atcgtgcgat
     1801 gatggagtac gtcccgatag tcggcgtcca gctccccgcg ctctggctca cataatcagt
     1861 cacccctgga ttttttcacg catgatcacc aacggttcac ccgccgggac gtcccggccc
     1921 gcgacttcgg acatcctccg gagtcgacca attcgtctcg ccatcttcgg cgacgccgag
     1981 aacgcccggc ggctgcggga tctccccgac gtcgatatcc gctggctcgt caaacgagac
     2041 agggcggacg gctcgatcgc gggagcgctc gccgatcgcg atgtcgatgc cgtgctgatc
     2101 gacctcccga tcgcgccgtg gtacgacatc gcggtggatg ccgcgcgcgc cggcaagcat
     2161 ctctttctcg ccgcgccgct tgcgatcacc gtcgatgacg cgcgggcgct cgtggatgtc
     2221 tgcgccgatg ccggggtatc gatcgtcatc gacaacacgg tgcgcttcac gccggaatac
     2281 cggaagatcg cccagatcat cgccgccggg gaactcggtc agatcgcgac gatccgcacc
     2341 aaccgggtga tcgcgccgcc cgaaaccggc gatatcatcg atcacgcctt gcgtcacgat
     2401 ctcgacgcct tgcgcaacat cttcggcgac atccgccgcc ttcacgctca tgccgcgatg
     2461 tccaggctgg gtcgcccgcg tcaggagttc gccgccgcct cgatccgctt cgcgaacggc
     2521 gcgatcgccc atatcgaggg acgtctctcg ccgacgacac cgcgcgtcgc gatcgagatc
     2581 gccggccaga acgggatcct cagcttcgac agcgatgccg catcgacctt gacgatcgaa
     2641 cggacgacag cgacgccgtt gcggactctc gcccgacccg ccgtcgcgac gccggagcag
     2701 atctcgctgc agaacttcct cgccagcctg cgcgccgagg aggccgagat cacgaccggc
     2761 gccgaggcga tcgagacgct ccggctcgtc ggcaagcttc gcgagtcgat cgcgacgggc
     2821 gcgccggttg ccgcatcctg caccgaggag agcccggtat gacctccacc att
//
LOCUS       DASTMD010000829         3216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_970175, whole genome shotgun sequence.
ACCESSION   DASTMD010000829 DASTMD010000000
VERSION     DASTMD010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970175"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1091
                     /locus_tag="VFQ54_12295"
     CDS             69..1091
                     /locus_tag="VFQ54_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0165816.1"
                     /translation="MRTQPTDLSLSLLRDTVIEHWGLTDPQLTYLPLGFGSHHWNVVD
                     AASGERWFATVDDHRNPRMVVTPAASLDALRRAMRTAAALHDEAGLAFVLAPTPNREG
                     GVLRSIGATAYSIVLFPYVEGTSAPYGAFASEADRLETLRLVGRVHAATGLVPEGLPR
                     RETFLIPRREDLFVLLDDLEAPWESGPYAEGARDLLRANRGDILRAFVRYDALVAAVE
                     ADPEPWVVTQGEPHAGNILRTTAGMLLLVDWDTCMLGPKERDLWMLDEGPATDWTVYL
                     EETGATTVSRRVIDLYRMWWDLCEISEFASWFRAPHEATEDTAIGWNALNGYLPIDPA
                     YLELAG"
     gene            1310..2824
                     /locus_tag="VFQ54_12300"
     CDS             1310..2824
                     /locus_tag="VFQ54_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEU0165817.1"
                     /translation="MTDEQKKPTLTTRQGHPVHNNQELRTVGNRGPATMENYHFLEKI
                     SHFDRERIPERVVHARGFVAHGEFEAYGTIGDEPASVYTRAKLLQEKGKKTPISIRFS
                     TVAGGRDSSEVARDPRGFAVKFRTEDGNWDLVGNNLPVFFIRDATKFPDFIHSQKPNP
                     VNFNRQDANRVFDFFAASPEAMHMITWLFSPRGIPANYRQQDGFGVNTYRMVNAAGEG
                     VLVKYHWKSQQGIESLTAAQAAEIQAHELGSHSKDLYEAIEAGNYPKWELNVQIMSDD
                     EHPELDFDPLDDTKTWPEDVFPLRPVGVMTLNRNVDNFFNESEQIAFGTGVLVDGLDF
                     SDDKMLVGRTFSYSDTQRYRVGPNYLQLPVNRPLSPVNTNQDGGQMSYGVDNRGTDPQ
                     VTYEPSVLAGTVEADDSYRGYEPEVSGKVQRAPIDRTNNYGQAGERYRTIEQWERDDL
                     VLNLTNELGKADIRLQEIMLGHITACDAEFGQRVATGLGLPVPAGALAAAGNDD"
BASE COUNT          600 a         1047 c         1028 g          541 t
ORIGIN      
        1 gctagagtga gtgacggacg ggattcgagt gtgcgatctt catcccgccg tgaagggatg
       61 gatttctgat gcgcacacag ccaaccgatc tctcgctctc gctgcttcgt gacaccgtga
      121 tcgaacactg gggattgacc gatccccagc tgacctatct tccgcttggc ttcgggagtc
      181 accactggaa cgtggtggac gcggcttccg gggaacgctg gttcgcgacc gtggacgacc
      241 atcgcaaccc gcggatggtg gtgaccccgg cggcctccct ggatgcgctt cgccgggcga
      301 tgcgaacggc ggccgcgctc cacgacgagg cgggtctcgc gttcgtcctc gctccaacac
      361 ccaatcgcga gggaggggtg ctgcgttcga tcggggcgac ggcctactcg atcgtgctct
      421 tcccgtacgt cgaggggacg tccgctccct acggggcgtt cgcctcggag gccgaccggc
      481 tggagacact gcgccttgtc gggcgcgtgc acgcggcgac gggtctcgtc ccggagggct
      541 tgccgcgtcg cgagacattc ctgatcccgc gccgggagga tctgttcgtc ctcctcgatg
      601 acctcgaggc accgtgggag agcggtccgt atgcggaagg tgcgcgggac ctcctgcggg
      661 cgaatcgcgg cgacatcctc cgcgccttcg ttcgctacga cgccctggtc gccgcggtcg
      721 aggccgatcc ggagccgtgg gtcgtgacgc aaggtgagcc gcacgccggc aacatcctgc
      781 ggaccacggc cggcatgttg ctgctggtcg actgggatac ctgcatgctg gggccgaagg
      841 agcgcgatct ctggatgctc gacgagggac cggcgaccga ctggaccgtc tatctcgaag
      901 agacgggggc gaccacggtg tcacgacgtg tgatcgatct ttaccggatg tggtgggatc
      961 tctgcgagat ctcggagttc gcctcctggt ttcgggcgcc gcacgaggcg acggaggata
     1021 ccgcgatcgg gtggaacgcc ctgaatggct acctgccgat cgatcccgcg tatctggagc
     1081 tggccggcta agagggattc ccacgctgga tcgcgatgcc gatcggctcg tgtacgaccg
     1141 gtagggccag gtcaccgtgg ccgtggctat ccaggaatca ttcgcatcac aaattgacga
     1201 ttgtgtaaga tagaagctga atatccagtg gcacgcgcct ccctgcaacg tcccgccgtg
     1261 ccgacgattt tggccggatc atcgatcccg ccaagccagg agcagacaga tgacagacga
     1321 acagaagaag ccgacgctca ccacacggca gggtcatcct gttcacaaca accaggagct
     1381 ccgcaccgtc ggcaaccgcg gcccggcgac gatggagaac taccacttcc tcgagaagat
     1441 cagccacttc gaccgcgagc ggatccccga gcgcgtcgtc cacgcgcgcg gtttcgtcgc
     1501 ccacggtgag ttcgaggcgt atggcacgat cggcgacgag ccggccagcg tctacacccg
     1561 tgccaagctg ctccaggaga aggggaagaa gacgccgatc agcatccgct tctcgacggt
     1621 ggccggtggc cgcgattcct ccgaggtcgc gcgcgatccg cgtggcttcg cggtgaagtt
     1681 ccgcaccgag gatggcaact gggatctcgt cggcaacaac ctgccggtct tcttcattcg
     1741 cgatgccacc aagttcccgg atttcatcca ctcgcagaag ccgaatccgg tcaacttcaa
     1801 tcgccaggac gcgaaccgcg tcttcgactt cttcgccgcc tcgcccgagg cgatgcacat
     1861 gatcacgtgg ctcttctcgc cgcgcggcat cccggcgaac tatcgtcagc aggacggctt
     1921 cggcgtcaac acctaccgga tggtgaacgc tgccggcgaa ggcgtgctgg tcaagtacca
     1981 ctggaagagc cagcagggga tcgaatccct caccgccgcc caggccgccg agatccaggc
     2041 gcacgagctg ggcagccact cgaaggatct ttacgaggcg atcgaggccg gcaactatcc
     2101 gaaatgggaa ctcaacgtcc agatcatgag cgatgacgag catcccgagc tcgacttcga
     2161 tccgctggat gacaccaaga cctggccaga ggatgtcttc ccgctgcgcc cggtcggcgt
     2221 gatgacgctc aatcgcaacg tcgacaactt cttcaacgag agcgagcaga tcgccttcgg
     2281 caccggcgtg ctcgtcgacg gcctcgactt ctcggacgac aagatgctgg tcggtcgcac
     2341 cttctcctac agcgacaccc agcgctaccg cgtcgggccg aactatctgc aactgccggt
     2401 gaaccggccg ctctccccgg tgaacacgaa ccaggatggc ggtcagatgt catacggcgt
     2461 cgacaaccgc ggtaccgacc cgcaggtcac ctacgaaccg tcggtgctcg ccggcaccgt
     2521 cgaggcggac gattcctacc gcggctacga gccggaggtg agcggcaagg tccagcgggc
     2581 gccgatcgac cgcacgaaca actacggtca ggcgggcgag cgctaccgca cgatcgagca
     2641 gtgggagcgg gacgatctcg tgctcaatct cacgaacgag ctcggcaagg cggatatccg
     2701 cctgcaggag atcatgctcg gtcacatcac ggcctgcgac gccgagttcg ggcagcgcgt
     2761 cgccactggg ctcggcctgc cggtcccggc gggcgccctc gccgcggcgg gcaacgacga
     2821 ctagcggtgt gtcaccgctg aattgccatg gtgatctcgt agcgggccac cccgtgtggc
     2881 ccatgcggca aagccgcata tgatcgttgg catacggtgc caaccggcaa aacatcggtg
     2941 gcagaccact agaccatccg cttcaatcca gtagcacgat gaaggccggt cacgcatgtc
     3001 gcggccggtc cgcgtcgttt ggtggcatgc tgtcccggca tagaccaacc gaggcacagg
     3061 aggcgtgcac aatgacggca gcacttgacc tcaagaagga acgcaaggac ctgtacgctc
     3121 cgccggtcgc cgatttcgtg gcggtgacgg ttccggccac gacattcctt gctatcgacg
     3181 ggcacggcga tccaaataca agcgatacct accgag
//
LOCUS       DASTMD010000830         1994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_165229, whole genome shotgun sequence.
ACCESSION   DASTMD010000830 DASTMD010000000
VERSION     DASTMD010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165229"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            663..887
                     /locus_tag="VFQ54_12305"
     CDS             663..887
                     /locus_tag="VFQ54_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165818.1"
                     /translation="MMQIHEGQCGLCSHFGEHDKSVAPQLIQIRLKHEAPENLVERCG
                     LPANLDLDLKVTPNSGCSGFSPAPVSEHDA"
     gene            1054..>1994
                     /locus_tag="VFQ54_12310"
     CDS             1054..>1994
                     /locus_tag="VFQ54_12310"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0165819.1"
                     /translation="MTRLGVGVIGLGEVAQIIHLPILQQLSERYRIAAICDISPTLLD
                     AMGFRYGVPADARHLDFHDLVARDDVDVVFVLNSDEYHAECAIAAATAGKHVLIEKPM
                     ALTDAEAEGIIRARDAAGVQVMVGYMRRFAPAFVRAVEEVKALEKITYVRVRDIIGQN
                     RLFIEQSTNVVRPNDFTPEQAADRRARGAAAVREAIGDAAEQFGGTYRWLCGLSSHDL
                     SAMRELIGVPQRVISATRAPRDGFMVIVFQYDGFTAILETGGNLIPRFDAHLEVYSPK
                     TSIRIDYDSGYIRHLPTTLTIVTTDGVSLEEQVIRPT"
BASE COUNT          387 a          625 c          603 g          379 t
ORIGIN      
        1 cgatcgcgtc gtcctcgggt ccggtccaat cgaccgtacc gccgggaagc gcgagccagc
       61 cagcgccgaa atcctcgtcg tcaccgcgca cgatcatcag gtagcgaccg tcgcgcacga
      121 tcgcaacttc gacgttcacg atgaagcggt ccttggtcga ctccgacacg attcctccaa
      181 aaggtacaca tgtcacgata cgcacagtaa acgctcagga tacttgatcc gatccgccgt
      241 tcttcgcgcc tccggatcga ggatgcctct tgcgagaaac acctcccccc ttcccgtccc
      301 ggcccaggtg gtcgcctccc caacgtagcc gaaacacgtc gtgccttgat gatcccatag
      361 gcgattcccc tggccacgct ccgacgcggc tgccaggacg ggccctggcg atcgcccggt
      421 gcggggacgg acgcgccccg ccgatccatg gcgggagaga ttggaactca gagtcatatt
      481 gtcagaattg atttgataat ccaaagttac ttcatcggat atacgatttg aatggagagg
      541 gcattactca gaattggtaa agtggacatt aagcctcatt cttgatatca tagcgacacg
      601 acgttccgat gggtgaactt cgcttttcaa caccagacgt ccatcgaaaa aggagccata
      661 ccatgatgca gattcacgag ggccagtgcg gtctgtgcag ccacttcggc gagcacgaca
      721 agtccgttgc cccacagctg atccagattc gcctcaagca cgaggctccg gagaatctcg
      781 tcgagcgctg cggtctgccc gccaatctcg acctcgatct caaggtcacc ccgaacagcg
      841 gatgcagcgg attctcgccg gccccggtca gcgagcacga cgcctagttt ccctcgttcg
      901 cgaggcacgt tcggaaatcg gtcatcggcc cggtcgcaat tccagcgacc gggccgatgg
      961 cgttttctgc cgttctgcgc gacgatagcc gctcagcgtg gaatatcagg tggggcaacg
     1021 gcattacctg tgcaaagaac ggtggagatg accatgacgc gtctcggagt cggggtgatc
     1081 ggattgggcg aggtcgcgca gatcatccat ctgccgatcc tccagcaact ctcggagcgg
     1141 taccggattg ccgcgatctg cgatatctcg ccgacgctgc tcgatgcgat gggattccgg
     1201 tatggcgttc cggcggacgc ccggcatctc gatttccacg atctcgtcgc acgggatgat
     1261 gtcgatgtcg tcttcgtgct caacagcgac gaataccatg cggagtgtgc gatcgcggcg
     1321 gccaccgccg gcaagcacgt tctcatcgag aagccgatgg cgctgacgga cgccgaggcg
     1381 gaggggatca tccgggcgcg tgatgcggcc ggcgtgcagg tgatggtcgg ctacatgcgg
     1441 cgctttgccc ccgctttcgt gcgcgcggtc gaagaggtga aggccctcga gaagatcacc
     1501 tatgttcgcg tccgcgacat catcgggcag aaccggctct tcatcgagca atcgacgaat
     1561 gtggtccgtc cgaatgactt cacgccggag caggctgccg atcgacgcgc tcgcggcgcg
     1621 gcggcggtac gggaagcgat cggcgatgcc gcggaacagt tcggtggaac ctaccgctgg
     1681 ctctgcgggc tgagcagcca cgatctctcg gcgatgcggg agttgatcgg tgtgccgcag
     1741 cgggtgattt cggcgacgcg cgcaccgcgt gacggattca tggtgatcgt cttccagtac
     1801 gacgggttca cggcgatcct cgagactggc ggcaacctga tcccccgttt cgacgcccac
     1861 ctcgaggtct acagccccaa gacatcgatc cgcatcgatt acgactccgg ctacatccgt
     1921 cacctgccga cgacgctgac gatcgtgacg acggacggcg tctcgctcga ggagcaggtg
     1981 attcgtccga ccta
//
LOCUS       DASTMD010000831         5064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1775253, whole genome shotgun sequence.
ACCESSION   DASTMD010000831 DASTMD010000000
VERSION     DASTMD010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775253"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VFQ54_12315"
     CDS             <1..943
                     /locus_tag="VFQ54_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU0165820.1"
                     /translation="PTSENGDPVFSNTSQYDVSVTAPSAWEVAATGTVVANKVSGESR
                     TQQVITGPVRDFSIVANSQFDSVSDVVDGTTVTSWFLPGDDRVGNAVLTYASQAIALY
                     STLFGSYPYVEFDVVPVDLHGASGVEFPQLIYLAQSYYTADQSLDVPNYLDFTVAHEV
                     MHQWWYGMVGNNQYLHAFTDEGLTNFVSSLIYFTQEYGEDAGSKIFTGACLSSFDASV
                     KDGADQIVDTPTDAFDTTNDYVWAAYSKAPVGFDAIYNQIGEQAFVAGLADYLKENAF
                     GIAQPDDMLAAFEEASGQDLTALWKHWFDERAGAYDV"
     gene            936..2165
                     /locus_tag="VFQ54_12320"
     CDS             936..2165
                     /locus_tag="VFQ54_12320"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEU0165821.1"
                     /translation="MSRDSDAFATQPLPLSRGAAAGRWRTLAGSLTFQVLTILVVSRL
                     FLFGANWFTLRAFPRFGYYPAQLPDEFLPGTMWLDGWARWDTAHYVTIALHGYGGAGN
                     LSHDGGLGFFPLFPMMMKGLVAITGLTATEGRLAVAAIVIANVCFAVSVPLFARLVSA
                     RFDDEIARLATIFLCISPFSYFFSVGYSESLFLLLALLTFTFADRRWWIAAAIAVALA
                     DSSRIVGLALVPSLILLAWYSRVRWRELIAIAVISPLGTLGYFLYTWWKFDDFFGYFT
                     AQGHWGGWNEHVRHYLTLFLLHPRETLLGDPLNLVIVLNVILGIIWLISLPWVWKLLD
                     PGIATFTSLLVLGQGAMTWVSLGRYLLPAIGFYVIAAWFLSKPFWRGWPREIAIVGST
                     VMLTMLTILFGHGYWVI"
     gene            2273..2716
                     /locus_tag="VFQ54_12325"
     CDS             2273..2716
                     /locus_tag="VFQ54_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0165822.1"
                     /translation="MAITDIGHSAYGVSDIDASLSFYALLGITESFRLLRADGSLALV
                     YLHISGDRFIEIFPGGPAADTRADGKTVSYKHLCLLSDDIVNDVEGLRAKGVTIDVEP
                     KVGGDLNTQAWITDPDGNKIELMQIADDSPQRAVEQGREPVVGTL"
     gene            3036..4427
                     /locus_tag="VFQ54_12330"
     CDS             3036..4427
                     /locus_tag="VFQ54_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165823.1"
                     /translation="MQVLRLLQRQTIILALMAGAMISSTVAVAAAAGQPAATPITTST
                     ATVVDDDPKSTATTTPAATRPARSATATPTREATIRAAAEAPAQIGVWAYSCYDPNRA
                     GETQIVFGGVSPAGLSATLCAPVPGTIDVTISSPETGDSDSASNVGTGGYEQSLAVAP
                     GPGYTLSDNLSGQTSPAFELASLQIVEISLYQYTDVEPDVPATADVGNAFSMPVWIRS
                     CEDTERADGVDFIVDADFSVASTFCDVAAGYAGTVSLLALDGDVVQTVAFVDGQAIFG
                     YTGENQQGYIPRGYYSVRLDETGDTSETFLVGQTENGQHFGTEVTILRYVAQVVITPT
                     PPQITPDAPTPTVFPTSGVTPNPGLTPTAITSGGDDPTATVPTSTPPPTQTSTPTPTS
                     DAATPIATATADPSDPTTAPGKPTKPVKATSLPNTGQGRQESSDLSTWFLLTASMTAI
                     AGAAFVGLRGRRS"
     gene            4602..>5064
                     /locus_tag="VFQ54_12335"
     CDS             4602..>5064
                     /locus_tag="VFQ54_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0165824.1"
                     /translation="MKYGFVVSAGSAHQIIELAKEIEDAGWDGFFTWDGISIGTGGDI
                     FDPWAILAAAAVSTKRVTLGAMIFPLSRRRPWKVAREAITIDHLSDGRLVLPVGLGAL
                     DDAGFVRVHPEVSDRKQRAELLDETLAILDLAWTGEPVSFTGKHYQVEDVVF"
BASE COUNT          862 a         1761 c         1499 g          942 t
ORIGIN      
        1 tccgaccagc gagaacggcg atcctgtctt cagcaacacc tcgcaatacg atgtctcggt
       61 cacggcgccg tcagcctggg aggtcgccgc gaccggcacc gtcgtcgcga acaaggtctc
      121 cggcgagagc cggacccagc aggtcatcac cggccccgtc cgcgatttct cgatcgtcgc
      181 caacagccag ttcgactccg tctccgacgt cgtcgacggc acgacggtga catcgtggtt
      241 tctccccggg gacgatcgcg tgggcaacgc ggtcctcacg tacgcgtcgc aggcaatcgc
      301 cctctacagc acgctgttcg gctcctatcc ttatgtcgag ttcgatgtcg tgccggtgga
      361 cctccacggg gcgtccggtg tcgagttccc gcaactcatc tacctcgcgc agagctacta
      421 cacggcggat caatcgctcg acgtcccgaa ctacctcgat ttcaccgtgg cgcatgaagt
      481 gatgcaccag tggtggtacg ggatggtcgg caacaaccag tatctccacg ccttcaccga
      541 tgaggggctg acgaatttcg tctccagcct catctatttc acccaggagt atggggagga
      601 tgccggcagc aagatcttca ccggggcctg cctctcgtcc ttcgacgcga gcgtgaagga
      661 tggcgccgac cagatcgtcg atacgccgac ggacgcgttc gataccacga acgactacgt
      721 ctgggcggcc tacagcaagg cgccggtcgg attcgacgcg atctacaacc agatcggcga
      781 gcaggcgttc gtcgccgggc tcgccgatta cctgaaggag aacgccttcg gcatcgccca
      841 gccggacgac atgctcgccg ccttcgagga ggcgtcgggg caggacctca ccgccctctg
      901 gaagcactgg ttcgacgagc gcgccggggc gtacgatgtc tagggattcg gatgcgttcg
      961 cgacgcagcc actcccgctc tcccgggggg ccgcagccgg gcgctggaga acccttgccg
     1021 gatcgctgac gttccaggtc ctgacgattc tcgtcgtctc gcggctcttc ctcttcggcg
     1081 cgaactggtt cacgctgcgg gcgtttcccc gatttggcta ctatccggct cagcttcccg
     1141 acgagttcct tcccggcacg atgtggctcg acggctgggc ccgttgggat accgcccact
     1201 atgtcaccat cgccctccac ggctacggtg gggcgggaaa cctgagtcac gacggcggcc
     1261 tgggattctt ccctctcttt ccgatgatga tgaaggggct cgtcgcgatc accggactga
     1321 ccgcgacgga agggcggctc gccgtcgccg cgatcgtgat cgcgaatgtc tgcttcgcgg
     1381 tctcggttcc gctcttcgcc cggcttgtct ccgcgcgctt cgacgacgaa atcgcccgcc
     1441 tcgccacgat cttcctttgc atctccccgt tcagctattt cttctcggtc ggctacagcg
     1501 aatcgctctt cctgctgctc gcgctgctta ccttcacctt cgccgatcgt cgctggtgga
     1561 tcgccgctgc gattgccgtc gcgctcgccg attcctcgcg gatcgtcggt ctcgccctcg
     1621 tgccctcgct gatcctgctc gcctggtatt cgagggtgag gtggcgggag ctgatcgcaa
     1681 tcgcggtgat ctccccgctc ggcacgctcg gttacttcct ctacacctgg tggaagttcg
     1741 acgacttctt cggctacttc accgcgcagg gtcattgggg tggctggaac gaacacgttc
     1801 gccactatct cacgctcttc ctgctgcatc cgcgggagac cctcctcggc gacccgctca
     1861 atctcgtgat cgtgctgaat gtcatcctgg ggatcatctg gctgatcagc ctgccgtggg
     1921 tctggaagct gctcgatccc ggcatcgcga cctttacctc attgctggtc ctgggacagg
     1981 gcgcgatgac ctgggtctcg ctcggtcgct atctgctgcc ggcaatcggc ttctacgtca
     2041 tcgccgcctg gttcctctcc aagcccttct ggcgcgggtg gccgcgcgag atcgcgatcg
     2101 tcggttcgac cgtcatgctg acgatgctga cgatcctctt cggtcacggc tactgggtga
     2161 tctgaccgaa cccgatgaca gattgcatga actttgcgag aatgcggcgc cggaatcctt
     2221 cgcgcgtcga cgcatttcgc ggcgaccgtt gcctttcagg agtgatcaga ccatggccat
     2281 taccgacatc ggacattcag cctacggtgt cagcgacatc gacgcgtcgc tgagtttcta
     2341 tgcccttctc ggcattacgg aatccttccg cctgcttcgc gcggacggct cactcgccct
     2401 cgtctacctg cacatctcgg gcgatcgttt catcgagatc ttcccgggcg gcccggcggc
     2461 cgatacccgc gccgatggca agacggtcag ctacaagcac ctctgcctgc tctccgatga
     2521 catcgtcaac gatgtcgagg gcctgcgcgc caagggcgtg acgatcgacg tcgagccgaa
     2581 ggtcggcggc gatctcaaca cccaggcgtg gatcaccgat cccgacggca acaagatcga
     2641 gctgatgcag atcgcagacg attcgccgca gcgcgccgtc gagcagggcc gcgagccagt
     2701 ggtcggcacg ctctaatccc cgcatcccat cacgcaagga gtccccgtgg ccagaacagg
     2761 ccacggggac tctttgcgtc caccacatct cacaccgacg cggcgatcgc gttgaaaccc
     2821 ctgacgacgt cgaaggggag ggagcccaca cccgggctcc cgactttccg cgggcacatg
     2881 ctgcccgcac cggaccggat tccgttcagg ttggtacgcc tgaacaccta ccaaccatct
     2941 gaatacctgg ccgcgcacgc catccagccg ccattgatgt ggctggatga gttcgtgttg
     3001 cgccccggca gtgggagcgg acccggaggg acgtcatgca ggttcttcgt ttgttacaac
     3061 gccagaccat catcctcgcg ctcatggccg gcgccatgat ttcatcgacc gtcgccgtgg
     3121 ctgccgcagc cggacaaccg gccgcgacgc cgatcaccac ctcgaccgca accgtggtcg
     3181 acgacgaccc gaagtccacc gccacaacga cgcctgccgc caccaggccg gccagaagcg
     3241 caaccgcgac accaacccgg gaagccacca tccgtgctgc ggccgaagcc cccgcgcaga
     3301 tcggcgtctg ggcctacagt tgctacgatc cgaaccgtgc cggcgaaacc cagatcgtct
     3361 tcggcggcgt ctccccggcc ggtctcagcg cgacgctctg cgcgccggtg cccggcacga
     3421 tcgatgtcac gatctccagc cccgagaccg gcgattccga cagcgcctcg aatgtcggca
     3481 ccggcggata cgagcaatcg ctggcggtcg caccaggccc cggatacacg cttagcgaca
     3541 atctgagtgg gcagacctca ccggcattcg agctcgcctc cctccagatc gtcgagatca
     3601 gcctctacca gtacaccgac gtcgaaccag acgttcccgc caccgccgat gtcgggaacg
     3661 ccttctcgat gccggtctgg attcgctcgt gcgaggacac cgaacgcgct gatggtgtcg
     3721 acttcatcgt cgacgccgac ttctcggtcg cctcgacatt ctgtgacgtc gcggccggct
     3781 acgccggcac cgtctcgctg ctcgcgctcg atggcgatgt cgtgcagacg gtcgccttcg
     3841 tcgacggcca ggcgatcttc gggtacaccg gcgaaaacca gcagggatac attccccgcg
     3901 gctattacag cgtgcggctc gacgagaccg gcgacacctc cgagaccttc ctcgtggggc
     3961 agaccgagaa tggacagcac ttcgggaccg aggtgacgat ccttcgctac gttgcccagg
     4021 tcgtgatcac accgaccccg ccgcagatca ccccggacgc gcccacaccg acggtcttcc
     4081 cgacgtcggg agtgacgccg aatcccggac tgaccccgac ggcgatcacc agcggtggcg
     4141 acgatccgac cgccacagtc ccgacgtcga caccgccgcc aacccagaca tcgacgccga
     4201 cgccgacctc tgacgccgcg acgccgatcg cgacggcaac cgccgatccg tctgatccga
     4261 cgacggcgcc aggcaagccg acgaagccgg tcaaggcgac atcacttccg aacaccggcc
     4321 agggccgcca ggaatcatcc gacctctcga cgtggttcct tctcaccgcc tcgatgacgg
     4381 cgatcgccgg cgccgcgttc gtcggcctcc gaggtcgacg atcgtaggcc ggacccgttc
     4441 gatcgctcca gaccccggtc cgtctcttga gtggcagacc ggggtttcgt gtgggatcat
     4501 aggcaactca acggtcgatc atacgttctc atgaacacac gttctcgtcc cgacgacgcc
     4561 atgaagatgg acctcgccgg tagaacgtag gaggagtagg catgaagtac ggattcgtcg
     4621 tttcggcagg cagcgcgcac cagatcatcg aactcgccaa ggagatcgag gacgccgggt
     4681 gggacggctt cttcacgtgg gacggaatct cgatcgggac aggcggtgac atcttcgatc
     4741 cgtgggcgat tctcgccgcg gcggcggtct cgacgaaacg cgtcacgctt ggcgcgatga
     4801 tcttcccgct ctcccgccgc cgaccgtgga aggttgcccg cgaagcgatc acgatcgatc
     4861 acctctcgga cggtcgactg gttctgccgg tcggtctcgg cgccctcgac gatgccggat
     4921 tcgtccgcgt gcatccagag gtctccgatc gcaagcaacg ggcggagctt ctcgacgaaa
     4981 cgctcgcgat cctcgatctc gcctggacgg gagaaccagt ctccttcact ggcaagcatt
     5041 atcaggtcga ggacgtcgtc ttcg
//
LOCUS       DASTMD010000832         1520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_256039, whole genome shotgun sequence.
ACCESSION   DASTMD010000832 DASTMD010000000
VERSION     DASTMD010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256039"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VFQ54_12340"
     CDS             <1..1019
                     /locus_tag="VFQ54_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEU0165825.1"
                     /translation="GLYFFHNARELVARGSGPYFYLPKMESYLEARLWNDVFTAAQAE
                     LGIAHGTVRATVLVETFPAAFQMEEILYELRDHAAGLNAGRWDYIFSAIKTYRNRADK
                     LLPDRVQVTMAVPFMRAYTQLLVKTCHTRGAHAIGGMAAFIPTRRDPEANEIAMAAVH
                     RDKEREAGDGFDGSWLAHPGLIPIALEEFGKVLGERPNQKDVLREDVHVDAAQITTFD
                     VPGGKITENGLRLNVSVALQYINSWLNGVGAAAINNLMEDAATAEISRAQIWQWIRHQ
                     ATTDEVTAITRERYAQIRTEELAKLTASEPSRFPESAEILDGLILTDDFVAFLTLPAY
                     AYLD"
     gene            1188..>1520
                     /gene="uraD"
                     /locus_tag="VFQ54_12345"
     CDS             1188..>1520
                     /gene="uraD"
                     /locus_tag="VFQ54_12345"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEU0165826.1"
                     /translation="MAGAIDIVAIDDVNSRSESFLLERLGHLFEHSPWIVEEALRQRP
                     FASRQGFHEALVGVMNGAEAGRQLALIQAHPDLVGRAALAGTLTRSSTREQAAAGLGA
                     DDLTGEERD"
BASE COUNT          267 a          480 c          513 g          260 t
ORIGIN      
        1 tcggcctcta cttcttccac aacgccaggg agctcgtggc gcgggggagt ggaccgtact
       61 tctatctgcc gaagatggag agctatctcg aggcgcggct ctggaacgac gtcttcacgg
      121 cggcgcaggc ggaacttggc atcgcccacg gcacagtccg cgcgaccgtg ctcgtcgaga
      181 ccttcccggc cgccttccag atggaagaga tcctctatga attgcgcgat cacgccgccg
      241 gactcaacgc cggtcgctgg gattacatct tcagtgcgat caagacgtac cggaatcgcg
      301 ccgacaagct gcttcccgat cgcgtccagg tgacgatggc cgttcccttc atgcgggcat
      361 acacccagtt gctggtcaag acctgccaca cgcgcggcgc ccatgcgatc ggcggcatgg
      421 ccgccttcat cccgacgcgg cgcgatcccg aggcgaacga gattgcgatg gcggcggtcc
      481 atcgcgacaa ggaacgcgag gccggcgacg gcttcgacgg ttcctggctc gcccacccgg
      541 ggttgattcc gattgcgctc gaggagttcg gcaaggtcct cggcgagcgc ccgaaccaga
      601 aggatgtcct gcgcgaggat gtccacgtcg acgcggcgca gatcacgaca ttcgatgttc
      661 cgggtggcaa gatcaccgag aacggtctcc gtctcaatgt cagtgtggcg ctgcaataca
      721 tcaattcctg gctgaacggt gtcggcgcgg cggcgatcaa caatctgatg gaagatgccg
      781 cgaccgcgga gatctcgcga gcacagatct ggcagtggat tcgccatcag gcgacgacgg
      841 atgaggtgac ggcgatcacc cgcgagcgct acgcgcagat ccgcaccgag gaactggcga
      901 agttgaccgc gagcgagccg agccgattcc cagagtcggc cgagattctc gacggtctga
      961 tcctgaccga cgacttcgtc gccttcctga cgcttccggc gtatgcgtat ctggactagg
     1021 cacggctggc ggcggcggga gcacgatccg tgggcagacg ccaggcggga caggccgtcc
     1081 ggacctccgg tccggcagcg gcggtcccgc cctacgaacg actgcggtct caacggttct
     1141 tgcccgggag gttgcaggtc cccggggatc gtcggggtgg agttgagatg gcgggggcga
     1201 tcgatattgt tgccatcgac gatgtcaaca gccggtccga gtcgttcctt ctggagcgac
     1261 tcggtcatct cttcgagcat tcgccctgga tcgtcgagga ggccctgcgg caacgcccct
     1321 tcgcctcgcg tcagggattc cacgaggcgc tggttggcgt gatgaacggc gcggaggccg
     1381 ggcgtcaact cgcgttgatc caggcacatc ccgacctcgt cggacgtgcg gcgctggcag
     1441 ggacgctgac gcgatcatct acgcgcgaac aggccgcggc gggactcggc gccgacgatc
     1501 tgaccggcga ggagcgggat
//
LOCUS       DASTMD010000833         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2847069, whole genome shotgun sequence.
ACCESSION   DASTMD010000833 DASTMD010000000
VERSION     DASTMD010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2847069"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFQ54_12350"
     CDS             complement(<1..644)
                     /locus_tag="VFQ54_12350"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU0165827.1"
                     /translation="MTNGPWRLDPQLATDVLAMLRTARLVLLPTHQNVDADALASALA
                     VSRALGKLGVESRVLVSDGTLPRSLGFLPGIDDVLLYGKDELPDYDLLCLIDSSDLRR
                     LGGFYRDDPSRVDGRVPMINIDHHVTNDEFGAINIVAPGISSASEVVTDILAVWGVEL
                     TREMAQLLLAGIYGDTLGLRTDSVTSRTMRTAADLVDAGGNPSLIVDALFTLKS"
     gene            complement(641..1051)
                     /gene="rbfA"
                     /locus_tag="VFQ54_12355"
     CDS             complement(641..1051)
                     /gene="rbfA"
                     /locus_tag="VFQ54_12355"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU0165828.1"
                     /translation="MTRRTRQVGELLREELVDIIRREVKDPRVGFMSITNVEVPPDLR
                     TARVYVSVLGTDEEREATLAALRSAAKFIRFHLKPRLRMRQIPELEFRDDRSMEYAQQ
                     ISEALRVARARDLAIQAASEPAPAGEDVETKEGA"
     gene            complement(1112..>2220)
                     /gene="infB"
                     /locus_tag="VFQ54_12360"
     CDS             complement(1112..>2220)
                     /gene="infB"
                     /locus_tag="VFQ54_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU0165829.1"
                     /translation="GGDVPFVEVSARENMGIEDLLEVLLLVADVNELKANANQPASGV
                     VIEAKIDSNRGPVATVLVQTGTLNLRDVVVAGSTSGRIKAMFDDRGKRVRRATPSTPV
                     EILGLMEVPQAGDTFLVFEDEKAARQLADQRSAERRISSLSENRPVKLTELFDQVKEG
                     KTAELRVILKADVQGSLGAIQNALLKLNEDTDQGVQLSIQLAATGAISESDINLAVTT
                     RSIVVGFNIRPDVAGKRAADSAGVDVRFYTIIYELLDEIKKAMTGLLAPIFEDVTDGY
                     AEVRDTFKLPTNDVVAGLYVLDGKVIRNSRARVLRSGTVVFEGGIKSLKRFKEDAREV
                     AAGYECGLGLDGFNDIQIGDQMEFLHREQVARTV"
BASE COUNT          395 a          735 c          703 g          387 t
ORIGIN      
        1 ttcgacttca gggtgaacag ggcgtcgacg atcaacgacg gattgccgcc cgcatcgacg
       61 agatcggcgg cggttcgcat cgttcgcgag gtgacggaat ccgtgcgaag ccccagggtg
      121 tcgccgtaga ttccggccag caacagctgc gccatctccc gggtcaactc gacgccccag
      181 accgccagaa tgtccgtcac gacttcggac gccgaggaga tgcccggcgc gacgatattg
      241 atcgcgccga actcatcgtt ggtgacgtgg tgatcgatgt tgatcatggg aacgcggccg
      301 tccacgcgcg acggatcgtc gcgatagaag ccgccgaggc gacggaggtc ggagctgtcg
      361 atcaggcaga gcagatcgta gtccggcaac tcgtccttgc catagagcag aacgtcgtcg
      421 attccgggca ggaaaccaag cgaccgcggc aacgtgccgt cactcacaag gacgcgggat
      481 tcgacgccga gcttgccgag cgcgcgcgaa accgccagcg cggatgccag ggcgtccgcg
      541 tcgacgttct gatgcgtcgg gagcagcacg aggcgagccg tacgcagcat cgccagaaca
      601 tccgtcgcga gctgcgggtc cagccgccag gggccgttcg tcatgcgcct tccttcgtct
      661 cgacgtcctc cccggccggg gccggttcgg atgctgcctg aatggcgaga tcgcgagcgc
      721 gcgcgacccg cagggcttcg gagatctgct gcgcgtattc catcgagcga tcgtcacgaa
      781 actcgagttc ggggatctgc cgcatccgca ggcgaggctt gagatggaag cgaatgaact
      841 tcgccgccga gcgcaaggcg gcaagcgtcg cctcgcgctc ctcgtccgtc cccaggacgc
      901 tgacgtaaac ccgtgccgtg cgcagatcgg gggggacctc gacattcgtg atgctcatga
      961 agcccacgcg cggatccttg acctcacgac ggatgatatc gacgagctcc tcacggagaa
     1021 gctcgcctac ttgtctggtg cgtcttgtca caacgtgctc atcactccgc ggtcagtccc
     1081 gaagggaccc tccgctggag atcccccgga cctagacggt ccgagcgacc tgttcgcgat
     1141 gcaggaactc catctggtcc ccgatctgga tgtcattgaa gccgtccaga ccgagaccac
     1201 actcgtatcc cgccgccact tcacgcgcat cttccttgaa gcgcttgagc gacttgatgc
     1261 caccctcgaa gacaacggtt ccgctccgca ggacgcgcgc cctcgagttg cggatgacct
     1321 tgccgtcaag gacgtacagg cccgcgacga cgtcgttggt cggcagcttg aaggtatcgc
     1381 ggacctcggc gtagccgtcg gtgacgtcct cgaagatcgg tgcgagaaga ccggtcatgg
     1441 ccttcttgat ctcgtcaagg agttcgtaaa tgatcgtgta gaagcggacg tcgactcccg
     1501 cggaatcggc ggcacgcttg ccggccacat ccgggcggat gttgaagccg accacgatgc
     1561 tccgcgtcgt cacggcgagg ttgatatcgg actcgctgat cgcgcccgtc gccgcgagct
     1621 ggatcgagag ctggacgccc tgatccgtat cctcgttcag cttcaggagg gcattctgga
     1681 tcgcaccgag cgatccctgg acgtctgcct tcaggatcac gcgaagctcg gccgtcttgc
     1741 cttccttcac ctgatcgaag agctcggtca gcttgaccgg ccggttctcg ctgagcgacg
     1801 agatgcgacg ctccgccgac cgctggtcgg ccagctgccg ggcggccttc tcgtcctcga
     1861 agaccaggaa ggtgtcaccc gcctgcggga cctccatcaa gccaaggatc tcgaccggcg
     1921 tgctcggggt cgcgcgacgg acgcgcttcc cgcgatcgtc gaacatcgcc ttgatccgac
     1981 ccgaggtcga acccgcgacg acgacatcgc gcaggttgag cgtgcccgtc tgcacgagca
     2041 cggtcgccac cggaccgcgg ttgctgtcga tcttggcctc gatcacgacg ccactcgccg
     2101 gctggttggc attcgccttc agctcgttga cgtcggcaac gaggagcaga acctcgagca
     2161 ggtcctcgat gcccatgttc tcgcgcgccg agacctcgac gaaggggacg tcgccgcccc
//
LOCUS       DASTMD010000834         1641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1508692, whole genome shotgun sequence.
ACCESSION   DASTMD010000834 DASTMD010000000
VERSION     DASTMD010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508692"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFQ54_12365"
     CDS             complement(<1..336)
                     /locus_tag="VFQ54_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0165830.1"
                     /translation="MTDGTIGYERVQAVAGRVIDEVERVIVGKRAVIEQVMVAILCEG
                     HVLLEDVPGIGKTMLARSISVALGGELGRVQFTPDLLPTDITGSLVFDPRSAEFSYRP
                     GPILVNVLLA"
     gene            complement(559..693)
                     /locus_tag="VFQ54_12370"
     CDS             complement(559..693)
                     /locus_tag="VFQ54_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165831.1"
                     /translation="MLIAIAFILFAAMVASWMILPLGPSATKPADAPKAAPALQGHGD
                     "
     gene            complement(781..>1641)
                     /locus_tag="VFQ54_12375"
     CDS             complement(781..>1641)
                     /locus_tag="VFQ54_12375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU0165832.1"
                     /translation="AERKSFKAFVLFSLLWSTIVYSLLAHWVWGGGWLYKVGAIDFAG
                     GTVIHITSGVAALVTAIVLGKRKSFGTADADPHNLTLTVLGAGLLWFGWFGFNAGSAL
                     AANGQAASAFVTTNIATAAAALAWMSFSWWRHGHPSVLGAAAGAVAGLVAITPACGFV
                     GPMAAIVIGLVAGIVCPFALDFVKERLQIDDALDVFAVHGVGGIWGALATGLFAEKKF
                     GGVDGLFFGRPEQLWYQVVAVAAAIVVSGVGTFIILKLVNLVFPLRVEESEEVLGLDA
                     SVHGEAAYQL"
BASE COUNT          343 a          523 c          542 g          233 t
ORIGIN      
        1 cgcgaggagg acattgacga ggatcggccc cggccggtac gagaactcgg cgctgcgcgg
       61 atcgaagacg agcgagccgg tgatatccgt cggcaggagg tccggcgtga actggacgcg
      121 tccgagctcg cctccaagcg cgaccgagat cgaccgcgcg agcatcgtct tgccgatccc
      181 cgggacatcc tcgagcagga cgtgcccttc gcagaggatg gcgaccatga cctgctcgat
      241 caccgcccgc ttgcccacga tgacgcgctc gacctcgtcg ataacgcgtc ccgcaaccgc
      301 ctgtactcgt tcgtacccga tcgtgccgtc tgtcatgcgt gccgagcccc ttggttccgc
      361 gaggaagatg gtgaggtcag acgagtgtgc ccgctctttc caggacgctc gaaaggaata
      421 ccttcgtcag tataggagag tcgtcgaccc tccggaagtc cgtgccggcg ccaccagacg
      481 cccgtgacgc aacgaagcgg tccgtccgga ggtacccgga ggaccgctac tgtcgagaga
      541 attcggatgg aggcaggatt agtcgccgtg gccctggagc gcaggcgccg ccttcggggc
      601 atcagccggc ttcgtcgcgc tgggaccaag tggcaggatc atccaggagg caaccatcgc
      661 ggcgaagaga atgaaagcga tcgcgatcaa catctcggaa ttcctttccc ggtttcccgg
      721 aagctaggta gtggaatggg gcgacggtga agccactgcc gcccgggtac gaacgaccgg
      781 ttagagctga taggccgctt cgccatggac cgatgcgtcc agaccgagca cctcttccga
      841 ctcctcgacg cggagcggga agacgagatt gaccagcttc aggatgatga aggtaccgac
      901 accggagacg acgattgctg ccgccaccgc aacgacctgg taccagagct gctccgggcg
      961 accgaagaag agaccgtcga cgccgccgaa cttcttctcg gcgaagaggc cggtcgcgag
     1021 ggcgccccag attccaccga caccatggac ggcgaagacg tcgagcgcat catcgatctg
     1081 gagccgctcc ttgacgaagt caagcgcgaa cgggcagacg atgccggcaa cgagaccgat
     1141 cacgatggcg gccatcggac cgacgaaacc gcaggccggg gtgatcgcga ccaggccggc
     1201 caccgcaccg gcggcagcgc cgaggacgct cgggtgaccg tgacgccacc aggagaagga
     1261 catccaggcg agggcagctg cggccgtcgc gatattcgtc gtgacgaagg cggaggcagc
     1321 ctggccgttg gcggcaagcg cgctaccggc gttgaagccg aaccagccga accagaggag
     1381 accggcgcca agcaccgtca acgtcaggtt gtgcggatcc gcatccgcag tgccgaacga
     1441 cttccgcttg ccgagcacga tcgcggtcac gagggcggcg acacccgagg tgatgtgaat
     1501 gacggttcca cccgcgaagt caatcgcgcc gaccttgtat agccagccac caccccagac
     1561 ccagtgggca aggagtgagt agacgatcgt cgaccagaga aggctgaaga gcacgaacgc
     1621 cttgaagctc ttgcgctcgg c
//
LOCUS       DASTMD010000835         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1060546, whole genome shotgun sequence.
ACCESSION   DASTMD010000835 DASTMD010000000
VERSION     DASTMD010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060546"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1235
                     /locus_tag="VFQ54_12380"
     CDS             252..1235
                     /locus_tag="VFQ54_12380"
                     /inference="COORDINATES: protein motif:HMM:NF013729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEU0165833.1"
                     /translation="MSDKAGDHIELFISHASEDKPTLVRPLAEKLRQWGVKVWYDEFS
                     LTPGDSLSRSLDKGLSVAQFGLVVLSPEFLKKPWPEYELRGLTNIEMYRRSKVIIPIW
                     HGVSPEQVMTFSPPLADKIAVVTDGKSVELIAEAVLRTIRPDLADRMSLLRAIRKPSP
                     DAEILSVPPEEIAVLPAPRQFRSEGGIILRSMLVTAVLREQTGLAGSIDEFLIDLYRD
                     IHPEAELPIWEGIAVAFAHVCHEYTLTTEQRTLVVRLLLTATLNEEEADKIAKLLPGD
                     IAALTTMWGEKVAAIRRLEQVTCVVGKDGAVRLVLPDDAPGTLESDASTHD"
     gene            complement(1419..>1629)
                     /locus_tag="VFQ54_12385"
     CDS             complement(1419..>1629)
                     /locus_tag="VFQ54_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165834.1"
                     /translation="VTETSALTKATTRATITVALAQPSEDADPAAAAEGLEVTEQQAE
                     LGVGSESTMTARMDASSGRRHHPEA"
BASE COUNT          306 a          513 c          490 g          320 t
ORIGIN      
        1 cgtgacctcg accagtccga ccgcggtacg catccgggta ccgaccttga gccgaggccg
       61 ttcccaccgg cgaaacgcca catcgatatc gccggcccgg atcgacgcca gttccgcggg
      121 acgtatctgc acgtcaccca gtccaccaca cgccacgatc catccgccgg tccgggcatc
      181 cccacgacgc tgaatgtcgg tgccggctga cgaggacatc gctgggtgag agtgttggct
      241 acagtcaggt catgagtgat aaggccggcg accacataga actcttcatt tctcatgcct
      301 ccgaggacaa gccgacgctg gtccggccac tcgccgagaa actgcgccag tggggagtaa
      361 aagtttggta cgacgagttc tcgttgaccc ccggtgacag tctgtcgcga tcgctggaca
      421 aaggtctttc cgtcgcgcag ttcggactcg tcgtgctgag ccctgaattc ctgaagaaac
      481 cctggccaga atacgagctg cgcggcctca ccaatattga aatgtaccgc cgaagcaagg
      541 tcatcatccc gatctggcac ggcgtgtcgc ccgagcaggt gatgactttc tccccgcccc
      601 tggccgacaa gatcgccgtg gttaccgacg ggaagtctgt cgaattgatc gccgaggcgg
      661 tgctacgcac catcagacct gatctggcgg atcgaatgtc gctgctccgt gctatccgga
      721 aaccgtcacc ggatgcagaa atactaagcg ttccccccga ggagatcgcg gtcttgccgg
      781 cgccccgcca gttccggagc gaagggggga taattctacg atccatgctg gtcactgcag
      841 ttctccggga acagaccggc ttggccggga gtatcgacga attcctcatc gatctctatc
      901 gggatatcca tccagaagcc gaactgccca tctgggaagg cattgccgtc gcttttgcac
      961 atgtttgtca tgaatatacg cttaccaccg agcagcgcac cctggtagtg cgcctgctgc
     1021 tgacggcaac tctgaacgag gaggaagcgg ataagatcgc gaaactgctt cctggcgaca
     1081 tcgcggcgct gaccacgatg tggggtgaaa aagttgccgc gatcagacgc ctggagcagg
     1141 tcacctgtgt cgttggcaaa gacggcgcgg tcagactagt gctccccgat gacgcgccgg
     1201 gcaccctgga gagcgacgcc tctacacatg attgaagtgg cgcgttggcg cttgggatcg
     1261 tcgccgtaga tgatcgagcc cctctccgca gcaacgagat gtcgggactc ggccgtgagt
     1321 gatccaggac cccgacgatg gggacggctc cgtcagtggt ggcgttggcc ggacggctcg
     1381 gcgctgggtg actccggaga tgtcagacga ccccctcttt acgcttccgg gtgatgccgc
     1441 cgaccacttg aggcatccat ccttgccgtc atggtgctct ctgatccgac ccccagttcc
     1501 gcctgctgct ccgtcacttc gagtccttct gccgcggcgg cgggatctgc gtcctcggat
     1561 ggctgggcca acgcgaccgt gattgtcgcc ctggtcgtcg ccttggtgag tgcactcgtt
     1621 tcggtcacc
//
LOCUS       DASTMD010000836         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1150741, whole genome shotgun sequence.
ACCESSION   DASTMD010000836 DASTMD010000000
VERSION     DASTMD010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150741"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..994
                     /locus_tag="VFQ54_12390"
     CDS             170..994
                     /locus_tag="VFQ54_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0165835.1"
                     /translation="MNRYTWRTGILEAAMLVITALFVFPLWLLISVGFRSSSDVATDP
                     VGVPHSLYLDNFINAWQVGGLGSAFVNSIYITVVAVAVLVILGASASYYFARSVGRAG
                     RRWYGIVAAGMMVPFQIGVLPLYRVFSELGLAGNPLSLIIFNAGIQLPLTVILYSGFM
                     RQIPVEYEEAARVDGANTFQIFWRIIAPLLLPVTGTVIILDGIAIWNEFFTPLLYLGG
                     TSNVTVPVQIYSFVGEYSSDWGQIFAGLLMASVPVLIVFFVFQRYMIRSFGSGLKG"
     gene            1232..>2046
                     /locus_tag="VFQ54_12395"
     CDS             1232..>2046
                     /locus_tag="VFQ54_12395"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0165836.1"
                     /translation="MKRSARRGIAVAVGMATALALFGCTSTTGGGSAPAEDKTITLMN
                     WEALAGSPYEAVFKQFTADTGIKIEYQFSASGSNYWPKTRTVLGSNNPPDIMRIDDDF
                     LAAYAATGRLQNLNSYMKKSKVKTSDYYDSVLNNTKQPDGSTVGWSLGIQPRVIYYNK
                     TMFQKAGLKLPPTTWSDEGWTWDDFLADAKALSVSGQQWGADIIDDSGFEMIYPVNNG
                     GTGRWSKNGKSFTLADKKDAEAVQCVADLTCKYGVQPAWSDLLQNGRGQELFAS"
BASE COUNT          394 a          653 c          606 g          393 t
ORIGIN      
        1 cgcagggcgg cccaggctat gcgacgcaaa cgctgtccac cctgctctac aacgaggcgt
       61 tcctctacaa caagtacggc tacggcatcg cattgggact catcgtcttc atcctgatcg
      121 ccgtgatctc cttcggacag atgcgcatat tcaaggatcg gactcagctg tgaacagata
      181 cacctggcgt acgggcatcc tcgaggcggc gatgctcgtc atcaccgccc ttttcgtctt
      241 cccgctctgg cttctcatct ccgtcgggtt tcggtcctcg agcgacgtgg cgaccgaccc
      301 cgtcggggtg ccgcactcgc tgtacctgga caacttcatc aacgcctggc aagtgggcgg
      361 cctcggatcc gcgttcgtga acagcatcta catcaccgtc gtcgccgtgg ccgtgctggt
      421 catcctcggc gcgagcgcaa gctactactt cgctcgctcc gtcggtcgtg ccggtcggcg
      481 ttggtacggc atcgtcgcgg cagggatgat ggtgccgttt cagatcggcg tactgcccct
      541 gtaccgggtg ttcagcgaac tggggctcgc cggcaatccg ctctcgctca tcatcttcaa
      601 cgccggaatc caactgcccc tgacggtcat cctgtacagc ggattcatga ggcagattcc
      661 cgtcgagtac gaggaggcgg cgagggtgga tggagcgaac acctttcaga tcttctggag
      721 gatcatcgct ccgcttcttc tgccggtcac cggcacggtc atcatcctcg acggaatcgc
      781 gatctggaac gagttcttca cgccgttgct ctacctcggc gggacgagca acgtcaccgt
      841 ccccgtgcag atctacagtt tcgtgggcga atacagcagc gactggggcc agatattcgc
      901 cggcctgctg atggccagcg ttcccgtcct gatcgtcttc ttcgtcttcc agcgctacat
      961 gatccgcagt ttcggcagcg gtctcaaggg ctgatctcgc aagcctcgtc ccggcaaatt
     1021 cgacgctcgc cccggcagcg gcgctgattc tgggcgccct tcagacctcg attaatttcg
     1081 ctgtgtgatt taagaatttt gccttgcgga ataaatgcaa ttcccttacc ttggtccccg
     1141 aggctcagag gcttcgccta cgggatcggc aagcccgcac atcgtgccgg agatccgcgt
     1201 gggctgatcg cataacgtcg aaggagacag catgaaaaga tcagcgagaa gggggatcgc
     1261 ggtcgccgtc ggcatggcca cggcgctggc cctcttcgga tgcacgagca ccacgggcgg
     1321 tggttcggcc ccagcggaag acaagaccat cacgctcatg aactgggagg cgctggccgg
     1381 ctccccctac gaggccgtct tcaagcagtt cacggcagac actggcatca agatcgagta
     1441 tcagttctcc gcctccggca gcaactactg gcccaagacg cgcacggtgc tcggttccaa
     1501 caacccaccg gacatcatgc gcatcgacga cgacttcctc gcggcgtatg cggcgacggg
     1561 acgcctgcag aacctgaaca gctacatgaa gaagagcaag gtcaagacga gcgactacta
     1621 cgactcggtg ctcaacaaca cgaagcagcc ggacggctcc accgtcggct ggagcctggg
     1681 catccagcca agagtcatct actacaacaa gacgatgttc cagaaggccg gactcaagct
     1741 cccgccgacc acgtggagcg acgaaggctg gacctgggac gacttcctcg ccgacgccaa
     1801 ggcgctctcg gtgtcgggcc agcagtgggg tgccgacatc atcgacgact cgggcttcga
     1861 gatgatctac ccggtgaaca acggcggcac aggtcggtgg tcgaagaacg gcaagtcgtt
     1921 caccctggcg gacaagaagg atgcggaggc cgtgcagtgt gtcgcagacc tcacctgcaa
     1981 gtacggtgtg cagcccgcgt ggtcggacct gttgcagaac ggacgcggcc aggagctctt
     2041 cgcctc
//
LOCUS       DASTMD010000837         1598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_702461, whole genome shotgun sequence.
ACCESSION   DASTMD010000837 DASTMD010000000
VERSION     DASTMD010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702461"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1598)
                     /locus_tag="VFQ54_12400"
     CDS             complement(<1..>1598)
                     /locus_tag="VFQ54_12400"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEU0165837.1"
                     /translation="LVRSLVQIAAASIHAFAHRLRRVDNVVWVVLVAVLGIAFIWNQR
                     TNVRQIVDVVRNANGWWLLAVFAAAIGLHLGFVFTQSLILETLGHRIPFLPAVTTYAE
                     RQVVATVVPLGSAPSLVVLARRLAPFGVTNQDAVYSVLLFSLIGHSSFIIYLLPVLLW
                     LILSHHATTAIIGAAILLMAVVMLAVGFAAALLSNRGIPAGLRARFPASVERFLDSVR
                     GHNLRPRDLSPACLVALSVDGFGVLAIFLALHAVGSDASLGVAAVGYAVGTLFLLIAP
                     VFQGIGVVEFSMTVVLQQLGVPASQALGATLIYRVAVVWLPVLFGIAFQARHQKRLIG
                     YPARLPAIWTGVNGLIAILSVMPHPTYRRISDIRRPEDVAQFDVVHLHNVSRSITLTI
                     GFLLILLSFGLWRRQRTAWLAAVILSAIVVVSHFLKGHDHIGGFLALASIVPLLIYRD
                     RFRVRSDRPTIQRGLTFSTAAIVLALAYGIFSLWFLDRHQFGYDFSLWSSIRQTVDLY
                     TSVNSAGLMPQTLYAHWLVNSFHL"
BASE COUNT          355 a          447 c          595 g          201 t
ORIGIN      
        1 caggtggaac gaattgacga gccagtgtgc gtagagcgtt tgcggcatca gcccggcact
       61 gttgacactc gtgtagagat cgacggtctg cctgatcgat gaccagagcg agaagtcgta
      121 gccgaactgg tgccggtcga ggaaccagag actgaagatg ccgtaggcga gcgcgaggac
      181 gattgccgcc gtcgagaaag tcagcccgcg ctggatcgtc ggccggtccg agcgcacccg
      241 gaagcggtcc cggtagatca gcaggggaac gatgctcgcg agggcaagga agccaccgat
      301 gtgatcgtgc cccttcagga agtgactgac gacgacgatc gcggagagga tcacggccgc
      361 gagccaggcg gtgcgttgcc gtcgccagag cccgaaggag agcaggatca ggaggaagcc
      421 gatcgtcagc gtgatcgatc gcgacacgtt gtgcaggtga acgacatcga actgcgcgac
      481 atcttccggc cgccgaatat cgctgattcg ccggtacgtg ggatggggca tgacggagag
      541 gatcgcgatc agaccgttca cccctgtcca gatcgcggga agtcgggccg gatagccgat
      601 cagtcgtttc tggtggcggg cctggaaggc gatgccgaag aggaccggca gccacaccac
      661 ggcgacgcgg tagatgagtg tcgctccgag cgcctgcgac gccggaacac cgagttgctg
      721 gaggacgacg gtcatcgaga actcgacgac accgattccc tggaagaccg gcgcgatgag
      781 caggaagagc gtccccaccg cgtagccaac cgcggcgaca ccgagcgacg catcggaacc
      841 gacagcgtgc agggcgagga agatcgcgag gacaccgaat ccgtcgaccg agagcgcgac
      901 gaggcaggca ggggaaagat cgcgggggcg cagattgtga ccgcgcacgg agtcgaggaa
      961 tcgctcgacg gatgccggga accgcgcccg gagaccagca ggaatgccgc ggttcgagag
     1021 cagggccgcc gcgaacccga ccgcgagcat gacgacggcc atgagaagga tcgccgcccc
     1081 gatgatcgcc gtcgtcgcgt ggtggctgag gatgagccag aggagcacgg ggaggagata
     1141 gatgataaag gacgagtgcc cgatcaaact gaagaggagc accgagtaga cggcgtcctg
     1201 gttggtgacg ccgaacggcg caaggcggcg cgcgaggacg acaagcgatg gggccgaacc
     1261 aagggggacc acggtcgcga cgacctggcg ctcggcgtac gtcgtgaccg ccggcaggaa
     1321 ggggatgcgg tgtccgagcg tctcgaggat cagcgactgg gtgaagacga atccgagatg
     1381 gagcccgatt gccgcggcga agacggcgag gagccaccat ccgttcgcgt tgcgcacgac
     1441 gtcgacgatc tggcgcacat tggtgcgctg attccagatg aaggcgatgc cgagcacggc
     1501 aacgaggacg acccagacga cattgtcgac gcggcggagg cggtgggcga aggcgtggat
     1561 cgacgcggcc gcgatctgga caagcgatcg gacgagtc
//
LOCUS       DASTMD010000838         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1419046, whole genome shotgun sequence.
ACCESSION   DASTMD010000838 DASTMD010000000
VERSION     DASTMD010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419046"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..935
                     /locus_tag="VFQ54_12405"
     CDS             108..935
                     /locus_tag="VFQ54_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU0165838.1"
                     /translation="MIDNDKIQNHLDGELLSSYVNGDPEQDERKRIEAHLAECDACRG
                     DLAGLRAVVDLLQQLPQYNPPRSFQLGSEHENHRKQPATLPAIRMLPIVRTLSIAAVI
                     AFLVVTGALFVNHDNGNDKTTFTGAALAPQETNSQVGSGTGGNSPTSQSAEDESPSQA
                     KKDTDDEQTSGSSQSLIDRGDAASADNSASFDEPAAAAPPQAAQSAQGVPTVTPSPQT
                     SQGQGAPLTAEPVDTSDDSSGTSWLATSIGLGILAIILIGLWLVLARMSKARRDGLT"
     gene            987..1781
                     /locus_tag="VFQ54_12410"
     CDS             987..1781
                     /locus_tag="VFQ54_12410"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEU0165839.1"
                     /translation="MSILARLLRRRSSRGFASLYAAVALVMLAAVSFAPLAARAQSTP
                     AADLSQGTITVNGTGVITVDPDMATISFGVENQSRLLEDAQTSNSTAITKVTKALTDG
                     GVDAKDIQTSSYYVYPISKYDDSGNYVGVTGYQVSTTLSVKVKDLDKLGTLLDSAVAA
                     GANFVYGISFSVEDPSKPADQARAAAVADARGKADTLAKAAGLVITGVYSINETSSPT
                     PMAQDYQAAASAADSAPMAKQVPISAGTTQVEVDIQIVFETTPANG"
     gene            1956..>2299
                     /locus_tag="VFQ54_12415"
     CDS             1956..>2299
                     /locus_tag="VFQ54_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165840.1"
                     /translation="MHIWKRLGVSMTILLAFTIAFGATGLVASRIDSPDRVQAATALP
                     APDTSTSLVTSAGDLHKSLAARSGTLRVVDLSAERQYDKAHIPGAIHAWWQDGMDPYA
                     AAYGEVLQVKVG"
BASE COUNT          450 a          751 c          711 g          387 t
ORIGIN      
        1 gcttgaccgg gcagagtgaa atcccatcag caccttgaac agttggaacc tcgaaatctc
       61 atggaacggg tcggcactcg cacgtgccgg cgttggtatg gtctggcatg atcgataacg
      121 acaagattca gaatcacctc gatggggagc tgttgagttc ctacgtgaat ggtgatcctg
      181 aacaggacga acgcaagagg atcgaagcgc atcttgccga atgcgatgcg tgccggggag
      241 atctcgccgg tcttcgcgct gttgtcgatc tcctgcaaca actcccgcag tacaacccac
      301 cgcgctcctt ccagcttgga tccgagcacg agaatcatcg caagcagccc gcgacactgc
      361 cggcgattcg catgcttccg atcgtgcgga cgctcagcat cgccgccgtg atcgccttcc
      421 tcgtcgtcac gggcgcgctc ttcgtcaacc acgacaacgg caacgacaag acgacattca
      481 ccggcgccgc cctcgcaccc caggaaacga acagccaggt cggaagcgga accggtggca
      541 atagtccgac gtctcagtct gcagaggatg aatcgccatc ccaggcgaag aaggacacgg
      601 acgacgagca gacgtccgga tcgagtcagt cattgatcga tcgcggcgac gcagcctcgg
      661 cggacaacag tgcgtcattc gatgagccgg cagccgccgc tcctccccag gccgcccaga
      721 gcgcgcaggg cgtacccacc gtcactcctt ctccccagac atcacaaggg cagggtgccc
      781 cactcaccgc ggagcccgtc gacacctccg acgattcgag tggcacatca tggctcgcaa
      841 ccagcattgg gcttgggatc ctggcgatca tcctgattgg actttggttg gtgcttgctc
      901 ggatgagcaa ggcccggcgc gatggattga cctgatttcg gcgatccatc gcgtcacatg
      961 acaggcctcg ggatggagga acttccatgt ctattctcgc gcgtctcctg cgacgtcgct
     1021 cgtcgcgcgg tttcgcttcg ctctacgccg cggtcgcgtt ggtcatgctc gcggcggtga
     1081 gcttcgcacc gctcgccgcc cgcgcccaat cgacgccggc ggcggatctc tcccagggga
     1141 cgatcacggt gaatggcacc ggggtgatca ccgtcgaccc ggatatggcg acgatctcgt
     1201 tcggcgtcga gaaccagtcc cggctgctcg aagatgcgca gacaagcaac tcgaccgcga
     1261 tcacgaaggt gacaaaggcg ctcaccgatg gtggcgtcga cgccaaggac atccagacgt
     1321 ccagctacta tgtctatccg atctccaagt atgacgacag tgggaactac gtcggcgtga
     1381 ccggctacca ggtcagcacg acgctctccg tcaaggtgaa ggacctcgac aagctcggca
     1441 cgctgctcga cagcgcggtc gcggcgggcg cgaacttcgt ctatggcatc tcgttctcgg
     1501 tcgaggatcc gtcgaagccg gccgatcagg cgcgggctgc ggccgtcgcc gacgcgcgag
     1561 gcaaggcgga cacgctcgcc aaggccgcgg ggctcgtgat cacgggtgtg tactcgatca
     1621 acgagacctc gtccccgaca ccgatggcgc aggactacca ggccgccgcg agcgccgcgg
     1681 actcggctcc gatggcgaag caggtgccga tcagcgcggg cacgacccag gtcgaagtcg
     1741 acatccagat cgtcttcgag acgacgcccg ccaacggata gcgatcccga gagatcgcga
     1801 atgcgggaat caggaatgcc cgggagcgac gccgctcccg ggcattcctg tgccattcgt
     1861 cgacatcgac cacaccgacg cgctagcctt ccgtagtgaa cgcgccgccg aagcaaaccg
     1921 gagacaacgt gggaccgatc ggagcgagag gatcgatgca tatctggaag cgactcgggg
     1981 tgagcatgac gattctgctc gcgttcacga ttgcgtttgg cgccaccggt ctcgtcgcgt
     2041 cacggatcga ttctcccgac cgggttcagg cggccaccgc cctgccggcg ccggacacct
     2101 cgaccagtct cgtgacctcc gccggcgatc ttcacaagag cctggcggcc aggtccggca
     2161 cgcttcgcgt tgtcgatctc tccgccgagc gtcagtacga caaggcgcat atcccgggcg
     2221 ccattcacgc gtggtggcag gatggaatgg atccgtacgc ggccgcctac ggggaagtcc
     2281 ttcaggtgaa ggtcggcga
//
LOCUS       DASTMD010000839         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2312362, whole genome shotgun sequence.
ACCESSION   DASTMD010000839 DASTMD010000000
VERSION     DASTMD010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2312362"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VFQ54_12420"
     CDS             complement(<1..1013)
                     /locus_tag="VFQ54_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEU0165841.1"
                     /translation="MPRAKLIELRGYLTTSSVADALGLSPYLVKARIADGTLPPATRV
                     SDAGVLLFDDEWLSAARDVLSVVPRRRRRRGNRVHIGVPSPERVLGHTMGESGWLPEW
                     ADIVRYFEEIERASDRVRVEELGTTTGGKPYIAVYISSPENLQPSSLERNRELLEQLW
                     DPRGLNREETDLLVSEARSVAIVLATQHSNEIGAALMTLDLAYELITQDDPATQSILQ
                     NTITVLIPSHNPDGVQMIAEWYAKWLGTEYEGIDLPWLYHLYTGHDNNRDWFMMTQAE
                     TKLYAELHNREHPQAVFDMHQMGRKGVRFMVPPFIDPLDPNQDPVIQQGFAALGSHIA
                     QRLT"
     gene            complement(1380..1805)
                     /locus_tag="VFQ54_12425"
     CDS             complement(1380..1805)
                     /locus_tag="VFQ54_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0165842.1"
                     /translation="MSGRPLTIQWQESADELYQRYQKERNGHRRVRLHAFWLLRRGYQ
                     IADVGQAVDVDYRTIQRWVSWYRAGGIDAVIRRTPGHGAPGRPSRLSPDQVKALIARA
                     STGTFRTVWEAVDWVQGEYGVTYTYTGMHALLNRNAKHD"
     gene            complement(2137..2565)
                     /locus_tag="VFQ54_12430"
     CDS             complement(2137..2565)
                     /locus_tag="VFQ54_12430"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU0165843.1"
                     /translation="MPNSFPPVTNIERERLMDAARRAATDAYAPYSHFPVGAAVLTED
                     GTIVAGFNIENASYGLTVCGERVATFTAIGAGHSTVRAVAISTPRSPGATPCGACRQV
                     LNEFKPGDGDLVVLTDGRDGIEETTLAALLPSAFGPRDLD"
     gene            2698..3432
                     /locus_tag="VFQ54_12435"
     CDS             2698..3432
                     /locus_tag="VFQ54_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165844.1"
                     /translation="MTDVYDDSGVSSNPPAGTEDMEYAAASFLGRVLGLEDDTDSLAG
                     DLSVIKRDRNAAIYTIQLESSVGPAAFLVYGYLLNERGGDGHTGQELFDSGLATLQQA
                     AERNSPGPRAVAHGASGEFGFIIATTPGTFRALAGDPDEPEPVLEPDPREFPNTEATD
                     RLRRDAADELLHLLRSANERSQQWLQAIQTASQGGDGGEDLLAFNESETELALFLLDN
                     ESIGDLLQTLNILVSTAQQHAANAID"
     gene            complement(3503..>4019)
                     /locus_tag="VFQ54_12440"
     CDS             complement(3503..>4019)
                     /locus_tag="VFQ54_12440"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEU0165845.1"
                     /translation="GAVKIYLGGPMFVSAEVRYNVWLAGELRSRGFDVYCPNESEPIN
                     DKTRSDITPRKIYDADREALEDANIYLCQVSEDSGTNWEAGYFDCLSRYVDSRKYLGV
                     IGLATDIRLAATPVPEQVGVDNLAWSINAFVIGGLQRSLGIVGSEDELFLRLDEIWDA
                     AEQGTLTDREA"
BASE COUNT          712 a         1327 c         1246 g          734 t
ORIGIN      
        1 gtcagtcgct gggcgatatg cgatccgaga gccgcgaagc cctgctggat cacggggtcc
       61 tgattcggat cgagcggatc gatgaacggg ggcaccatga agcggacgcc cttgcgtccc
      121 atctgatgca tgtcgaagac agcctgcggg tgctcgcgat tatggagttc cgcgtagagc
      181 ttcgtctccg cctgggtcat catgaaccag tcacggttgt tgtcgtgtcc ggtatagaga
      241 tggtagagcc atggcaggtc aatgccctcg tactcggttc ccagccactt cgcataccac
      301 tcggcgatca tctggacgcc atccggattg tggctcggga tgaggacggt gatcgtgttc
      361 tgaaggatgc tctgggtagc ggggtcgtcc tgcgtgatca actcgtaggc gagatcgagc
      421 gtcatcagag cggcgccgat ctcgttcgag tgctgcgtcg cgagaacgat ggcgacgctg
      481 cgcgcctcgc tgacgagcag gtcggtctcc tcgcggttca acccacgagg atcccagagc
      541 tgctcgagga gctcgcgatt gcgctcgaga ctgctcggct ggagattctc cggcgaggag
      601 atgtagacgg cgatgtaggg cttcccgccg gtggtcgttc cgagttcctc gacgcgcacc
      661 cggtccgagg cacgttcgat ttcctcgaag tagcggacga tgtccgccca ctccgggagc
      721 cagccggact cccccatcgt gtggccgagc acgcgctcgg gggacgggac cccgatatgc
      781 acgcggttcc cccgtcgacg gcgacggcgc gggacgaccg aaaggacatc ccgcgccgcg
      841 gagagccact cgtcgtcaaa gaggaggacg ccggcatcgg agacacgggt ggccgggggg
      901 agggtcccat cggcgattcg cgccttcacc agatagggag aaagcccgag ggcatccgcg
      961 actgatgagg tcgtcagata cccacgtagt tctatgagtt tggcccgggg cacgcttcac
     1021 ctgtttcttc actcaagcgg acgttcgttt tgttttcacg gaatttccgt cgtggcaaac
     1081 gctacaatcg cccgttgcac ggggatccga gcgcacgggt acatcatatg catattccgg
     1141 gatcgcacca gatgctgaat gatcccggta acggattcgt attgtcgcaa actttggggc
     1201 gtccgggaag aatttcagaa gattcttata atgttcagct attgacaggc cgctccgggc
     1261 cggggcggct tgccattcag gccagcgccg gtagttcgcg cgggcgtttg cctcggtcgg
     1321 gaatcccgat cactgcaggc gttctaccgg ctgggcctgt gtcgaggcga cgcgggccgc
     1381 tagtcgtgct tggcattccg attgagcagt gcatgcatgc ccgtgtacgt gtacgtcacg
     1441 ccgtactcgc cctgaaccca gtcgaccgct tcccagaccg tgcggaaggt tccggtcgac
     1501 gcgcgcgcga tgagcgcctt cacctggtcc ggactcagcc gggatggccg gcccggtgcc
     1561 ccatggcccg gggtccgcct gatcacggca tcgattcccc cggcgcggta ccacgacacc
     1621 catcgctgaa tcgtgcggta gtcgacatcc accgcctgcc cgacatcggc gatctggtag
     1681 ccccgtcgga ggagccagaa cgcatggagc cgtacgcggc gatgtccgtt gcgctccttc
     1741 tgataccgct ggtagagctc gtcggcggat tcctgccact ggattgtcaa tggtcgtccc
     1801 gacatggtcg tgcttcccct catcgaaccg ttcagccccg aactgggtcg atcttcgaac
     1861 tcggccgcca caccctccgg catcatctgg agcgtcgcgc tgccatcaac agccagaatg
     1921 acggtccctc ggccggcgca acaacgtgcc tcctcgaaac cggtactccg atcgtgcaat
     1981 cccgaagctg gccaatcttg ccgggactac gtgtatccga cagcgtcgga tatatcccta
     2041 tcgcaactct cctgccagcc tcgacgtgcg ctgctggacc ggcgcgcatc gatccgacgc
     2101 gggacagccc gcatcagcct ctccgacaat ggacgctcag tcgaggtcgc gcggcccgaa
     2161 agcggacggc aggagcgccg ccagggtcgt ctcctcgatc ccatcccgac cgtcggtgag
     2221 aacgacgagg tcaccgtccc cgggcttgaa ctcgttgagg acctgccggc acgcaccgca
     2281 cggcgtcgca cccggggaac gcggcgtact gatcgcgacc gcgcggaccg tactgtgacc
     2341 cgcgccgatc gccgtgaacg tcgccacgcg ctccccgcag accgtcagcc cgtagctggc
     2401 gttctcgatg ttgaatccgg cgacgatcgt gccgtcctcc gtcaatacgg ccgccccgac
     2461 cgggaagtgc gaatacggcg cgtaggcatc cgtcgccgca cgcctggccg cgtccatgag
     2521 tcgctcgcgc tcgatgttgg tcaccggcgg aaatgagttc ggcatctgca agtctccttg
     2581 ggcatccggc ttctcgcgtc ctggcaatcc gacgcaccgg aagtcgtgtg agagaatagg
     2641 tccatcgagg ttggcgattg ccaccgccgg caagcgactc aagggtggat cgggaagatg
     2701 acagacgtgt acgacgactc gggtgtttcg agcaaccctc cggcaggtac ggaggatatg
     2761 gaatatgccg ccgcgagctt tctcggtcgc gtgttggggc tcgaggacga caccgattcg
     2821 ctagccggag atctctccgt catcaagcgc gacaggaacg cggcgatcta cacgatccag
     2881 ctggagtcgt cggtcggtcc cgccgccttc cttgtctatg gctatctcct gaacgagcgc
     2941 ggtggcgacg gccacactgg ccaggagctg ttcgattccg gcctcgccac gctgcaacag
     3001 gccgcggaac gcaactctcc cggcccacgc gccgtcgcac atggcgccag cggtgaattc
     3061 ggcttcatca tcgcaacgac ccccggcacg ttccgggccc tcgccggtga tcccgacgag
     3121 ccggagcccg ttctcgaacc ggatcctcgc gagttcccga ataccgaggc taccgatcgt
     3181 ctccggcgcg atgcggcgga cgaactcctg catctcctcc gcagcgccaa cgagcgctcg
     3241 cagcaatggt tgcaggcgat tcagaccgcg agccagggtg gagatggtgg agaagacctc
     3301 ctggccttca acgaatccga gaccgaactc gccctcttcc tgctcgacaa cgagagcatc
     3361 ggcgatctcc tgcagacgct gaacatcctc gtttccaccg cccagcagca cgccgccaac
     3421 gccatcgact agggccgcgg cgcaatcgat cgcatacggc cggcccgcaa cccggtgata
     3481 cgcgtgaggg ttgtgcggcc gcttaagcct cgcgatcggt cagcgtcccc tgttccgcgg
     3541 catcccagat ctcgtcgagc cgcaggaaga gttcatcctc ggacccgacg atcccgagcg
     3601 accgctggag accgccgatc acgaacgcgt tgatcgacca ggcgagattg tcgacaccca
     3661 cctgttccgg cacgggcgtc gccgccagcc gaatgtccgt cgccagcccg atcaccccga
     3721 ggtacttccg ggaatcgacg tagcggctga ggcaatcgaa atagccagcc tcccagttcg
     3781 tgccggaatc ctcgctcacc tggcagagat agatattcgc atcctcgagc gcttcgcgat
     3841 cggcatcgta gatcttgcgc ggcgtgatgt ccgaccgcgt cttgtcgttg atcggttcgc
     3901 tctcgttcgg gcagtagaca tcgaatccgc gcgagcgcaa ttcacccgcg agccagacgt
     3961 tgtaacgcac ctcggccgag acgaacatcg gcccacccag gtagatcttc acggcgccc
//
LOCUS       DASTMD010000840         3668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_792107, whole genome shotgun sequence.
ACCESSION   DASTMD010000840 DASTMD010000000
VERSION     DASTMD010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792107"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFQ54_12445"
     CDS             <1..225
                     /locus_tag="VFQ54_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165846.1"
                     /translation="PAGGGTGLILRQYKQVGSGRDAQLVEEYVVDTATVRQIQSLHEQ
                     AAKELGQWTEKVNMSGGLKREYVIVTEETE"
     gene            225..635
                     /locus_tag="VFQ54_12450"
     CDS             225..635
                     /locus_tag="VFQ54_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165847.1"
                     /translation="MVALRADRPAAPNVHLVTEFRGAVGPLWKEHTGEIIISGPAGTG
                     KTRSILEWIHLRCSKEKLRVLFLRKTLESLKTSAMVTYTEKVLHDFDGKHSVLDGVSY
                     FGGNSILPSNFTYHATGSTMVMGGMDNPTKILPT"
     gene            651..1625
                     /locus_tag="VFQ54_12455"
     CDS             651..1625
                     /locus_tag="VFQ54_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165848.1"
                     /translation="MNEVTELSLDQWERLGNRTDRPDQNQDKPASLLLGDCNPDVPTH
                     WIKKRESDGVLKLWLTTHEDNPAMWDRKLKAWTRTGALYMKRLEMLTGVRYLRLKQGL
                     WVAAEGQVYETWNPDIHIAKTRYEIPYHWPRYWVVDFGYVHPFVWQAWARKPDGELVM
                     YREIYMTRRLVSSHARRILALTANEPRPTAIITDHDAEDRATFEEETGFSTRPATKNV
                     SGGIQAVANRLGNAHNPARIEFLPNSLDERDTDLADRGQPTSTIEEFPSYVWNLASNR
                     RKGEEPIKDYDHGMDATRYLIAYFDLKDGQTAELRAPTGSLRRYLAEQ"
     gene            1661..3190
                     /locus_tag="VFQ54_12460"
     CDS             1661..3190
                     /locus_tag="VFQ54_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165849.1"
                     /translation="MSALATGWNAGINDWRAGPQPSEFEILQQVYWERWQIYKGTIFA
                     DMLRNTAYLKDRRIYKGTGLLWNHTSRVVDFWGAMMYQGVLSTKPGQGAIPLAIGPEM
                     TDDQTNALLAVIDAIYKRWNWQQQMIYRPKMTAALGDCLTELIDDLDKRFVFPRIVWS
                     GFVKTVVLDDSGFLTSYELEYPVTEADDNGAVLDSYTYGKRVTKETFSYFKNGEPFDY
                     YDVGAVVENPYGFVPAIWDRHNALYGVRGDAAIDKSRQQLFSLNSLFSHSRDYQHKAF
                     FAPVVVTGRMSQENETTIDLSGEQQTASAMAQALSFVEAEAGADVKQVTFDVGRTLDM
                     LESIKEGILDNHPEATFFQKLAEATQVTGPGANTIILPVKGMVIAERSGMDANTVRLI
                     QMAISMGGMRVNNGDWGPTSELNRARRAFLPYTLDSYKNEEMEFTIEERDIIAPTEAE
                     RLEIIVLKESLQTQWGLEQAGEEPDVAKTILAQKQAALAGGLDIATSSEDLAKLDSEA
                     A"
     gene            3190..>3668
                     /locus_tag="VFQ54_12465"
     CDS             3190..>3668
                     /locus_tag="VFQ54_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165850.1"
                     /translation="MARGDAAFMTAVAQAIKLADDFSRSLGKRIRDLVAAFSPLSAAT
                     RSRLMQQIDAILNAAFGLTQRAALLSDLYLTILTATDAAASRPFTALFTRIETAVNLL
                     GPGLWNAVRSGMLGSPASDALARAYTATTSFGASRTAYLRTAKLDPNRRWVKGDGTS"
BASE COUNT          758 a         1147 c         1126 g          637 t
ORIGIN      
        1 ccggcagggg gcgggaccgg cctcatcctg cggcaataca agcaggttgg ctcgggtcgg
       61 gacgcgcaac ttgtcgagga atacgtcgtt gacaccgcga ccgtccggca gatccagtcg
      121 ttgcacgaac aggcggccaa ggaactcgga cagtggaccg agaaggtcaa catgagcggc
      181 gggctcaagc gggagtacgt catcgtcacc gaggagaccg agtgatggtg gctcttcgcg
      241 ccgatcgtcc agccgctccc aatgtccacc tggtgacgga gttccgcggc gccgttggtc
      301 ctctttggaa ggagcatacc ggggagatca tcatctccgg gccagcgggc accggcaaga
      361 cgcgatcgat tctcgagtgg atccacctgc gatgttcgaa agagaaactt cgcgttctct
      421 tcctccgcaa gacgctcgaa tcgctcaaga cctcggcgat ggtgacgtat acggagaagg
      481 tcctgcacga tttcgacggg aagcatagcg tcctcgatgg agtttcctat ttcggcggaa
      541 actctatcct gccatcgaac tttacctacc atgccactgg ctccacgatg gtcatgggcg
      601 gtatggataa ccccaccaag atccttccta cataatacga cctgatgtac gtgaatgagg
      661 tgactgagct ctcgctggac caatgggaac gtctcggcaa ccgcaccgat cgccccgacc
      721 agaatcagga caagccggcg tccctgttgc tcggcgactg caatcccgat gtgccaaccc
      781 actggatcaa gaagcgcgag agtgacggag ttctcaagct ctggctgacc acgcatgaag
      841 acaatccggc gatgtgggat cgaaagctga aggcgtggac ccgcaccgga gcgctctaca
      901 tgaagcgtct cgagatgctg accggcgtgc gctatctccg gctgaaacaa gggctgtggg
      961 tcgcggcaga gggacaggtc tacgagacct ggaatcccga cattcacatt gcgaagacgc
     1021 gctacgagat tccctatcac tggccgcgat attgggttgt ggactttggc tacgtgcatc
     1081 cgttcgtctg gcaggcgtgg gcacgcaagc cggatgggga gttggtcatg tatcgtgaga
     1141 tctacatgac ccgccggctc gtctcttccc atgcccgccg catccttgcc ctcaccgcga
     1201 acgaaccacg gccgacagcc atcatcaccg atcacgatgc cgaggatcgc gcaacgttcg
     1261 aagaggaaac cggattctcg acccgcccgg cgaccaagaa cgtgagcggt ggcatccagg
     1321 cggtcgcaaa ccggcttggc aacgcgcaca atcctgcgcg catcgagttc ctgccgaaca
     1381 gtcttgatga gcgggacacc gatcttgcag accgcggcca acccacgagc acgatcgagg
     1441 aattcccttc gtacgtctgg aaccttgcat cgaatcgacg caagggtgaa gaaccgatca
     1501 aggactatga ccatggcatg gatgcgaccc gctatctgat cgcctacttc gatctgaagg
     1561 atggtcagac cgcggagctc agggccccga ccggctcgct ccgtcgctat ctggcggagc
     1621 agtaacgatg agcggactga ccgcgtattg gagacgggcc atgagtgcac ttgcgacagg
     1681 gtggaatgcc gggatcaacg actggcgcgc cggtccccag ccgagcgaat tcgagatcct
     1741 gcagcaggtc tactgggaac gctggcagat ctacaagggc acgatcttcg ccgacatgct
     1801 ccgcaatacg gcctacctga aggaccgccg tatctacaag ggcaccggcc tgctctggaa
     1861 ccacacgtcc cgcgtcgtcg acttctgggg cgcgatgatg taccagggtg tcctctcgac
     1921 gaaaccgggg cagggggcga tccccctcgc catcggcccg gagatgacgg acgatcagac
     1981 gaatgccctg ctcgccgtca tcgatgccat ctacaagcgg tggaactggc aacagcagat
     2041 gatctaccgc ccgaagatga ccgccgcgct cggcgactgc ctgaccgagc tgatcgacga
     2101 tctcgacaag cggttcgtct ttccccgcat cgtttggtcc ggcttcgtga agaccgtcgt
     2161 cctcgacgac agcgggttcc tcacctcgta cgagctcgag tatcccgtta ccgaggccga
     2221 cgacaacggt gccgtcctcg attcctacac ctatggcaag cgggtgacga aggagacgtt
     2281 ctcctacttc aagaacggcg aaccgttcga ctactacgac gttggcgcgg tcgtcgagaa
     2341 cccgtacggc tttgtgcccg cgatctggga tcgccacaat gccctctacg gcgttcgagg
     2401 cgatgcggcg atcgacaagt cgcgtcagca gctcttctcg ctcaacagcc tcttcagcca
     2461 ttcccgggac taccagcaca aggcgttctt cgcgccggtt gtcgtcaccg gccgcatgag
     2521 ccaggagaac gaaaccacca tcgacctctc cggcgagcag cagacggcaa gcgcaatggc
     2581 gcaggcactc tcgttcgtcg aggccgaagc cggcgccgac gtcaagcagg tcacattcga
     2641 cgttggtcgc acgctcgata tgctggaatc gatcaaggaa ggcattctcg acaaccaccc
     2701 ggaggcaacg ttcttccaga agctcgccga ggcgacccag gtcaccggac cgggagcgaa
     2761 tacgatcatc ctgccggtca aggggatggt gatcgccgag cggtccggga tggacgccaa
     2821 taccgtacgc ctgatccaga tggcaatcag catgggcggg atgcgggtca acaacggcga
     2881 ctggggtcca accagcgagc tcaaccgggc acggcgtgcg ttcctgccgt acaccctcga
     2941 cagctataag aacgaggaga tggagttcac gatcgaggag cgcgacatca tcgcgccgac
     3001 cgaagcggag cgtctcgaga tcatcgtgct caaggagtcg ttgcagacgc agtgggggct
     3061 tgagcaggcc ggcgaggagc cagacgtcgc gaagacgatc cttgcccaga agcaggccgc
     3121 gctcgctggc ggactcgaca ttgcgacgag tagcgaggat cttgccaaac tggatagcga
     3181 ggcggcctga tggcacgcgg cgatgcggcg ttcatgaccg ccgtcgcgca ggcgatcaag
     3241 ctggctgacg atttctcccg cagcctcggc aagcggattc gcgatctcgt cgcggccttt
     3301 tcgcccctgt cggccgcgac gcgttcccgc ctgatgcagc agatcgacgc aatcctcaac
     3361 gcggcgtttg gtctgacgca acgcgcagcc ctgttgagcg atctgtacct gacgatcctc
     3421 accgcgaccg atgctgcggc ctcgcggccc ttcaccgcgc tcttcacgcg gatcgagacc
     3481 gcggtcaacc tgctgggacc ggggctgtgg aacgccgtac gatccggcat gctcggatcg
     3541 ccggccagtg acgcgctcgc gcgcgcctac acggcgacga cgtcgttcgg ggcgtcacgg
     3601 actgcgtatc tccggacggc gaagctcgat ccaaaccgcc gatgggtcaa gggggacggg
     3661 acgtccta
//
LOCUS       DASTMD010000841         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2489170, whole genome shotgun sequence.
ACCESSION   DASTMD010000841 DASTMD010000000
VERSION     DASTMD010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2489170"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2399
                     /gene="uvrA"
                     /locus_tag="VFQ54_12470"
     CDS             <1..2399
                     /gene="uvrA"
                     /locus_tag="VFQ54_12470"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU0165851.1"
                     /translation="FVRVRVDGEVRELEEQIKLTKSRKHSIDVVVDRMVIRHPELEEG
                     ATDLDLEAHPDRVRLIDSMEVALRMSSGSAIVQIIDGEELTFSEHFACPHCGLSFGEL
                     EPRNFSFNSPHGACPECTGLGTTMEFDPELVLPNRNLTIAQGAIVPWMRSGMENSSWY
                     ASLIEAVAEQYHFSIHEPIKDLPAEALGILLYGSNGEAVTAQYASKNGRKRSYSVVFE
                     GVIPNLKRRYQQTQSDWVKEDIERYMSTRPCPVCHGARLKPEVLAVTVNEASIVDVSR
                     MPIHEAQQWFNLLGAEPTERDAILSNREFLIARQILKEIRERLGFLIDVGLNYLTLER
                     SANTLSGGEAQRIRLATQIGSSLMGVLYILDEPSIGLHQRDNAKLIRTLERLRNIGNT
                     LIVVEHDEDTMRAADWIVDIGPGAGVHGGYVVAEGTMEQIAATEGSITGDFLSGRRSI
                     PVPATRRKGNGKEIVIRGAKENNLRDVTVHFPLGTFTCITGVSGSGKSSLITDTLYKR
                     LAQELHHAKDKPGSHDAMEGIKHLDKVIDINQSPIGRTPRSNPATYTGLFTPIRELYA
                     SLPEAKTRGYKAGRFSFNVKGGRCEACKGEGIIQIEMNFLPDVFVPCEVCHGARYNRE
                     ALEILYKGSSIADVLDMTVAQALDFFENVPALRNKLQTLFDVGLGYVKLGQPATQLSG
                     GEAQRVKLASELSRRPTGRTMYILDEPTTGLHFADIEKLLVILQRLADGGNSVMVIEH
                     NLDVIKSADWIIDLGPEGGSGGGMIIAEGTPEAIAETPHSYTGEFLRPLLPQEAIAAD
                     "
BASE COUNT          490 a          778 c          753 g          399 t
ORIGIN      
        1 gtttcgtccg tgtccgcgtc gacggcgagg ttcgcgaact cgaagagcag atcaagctca
       61 cgaagtcgcg gaagcactcg atcgacgtgg tggtcgatcg catggtgatc cgccatccgg
      121 aactggagga gggcgcaacc gacctcgatc tcgaagcgca tccggatcgc gtccgcctga
      181 tcgactcgat ggaagtcgcg ctgcgaatgt cgagcggttc ggcaatcgtc cagatcatcg
      241 acggcgagga gctgaccttc tccgagcact tcgcctgccc gcactgtggg ctcagcttcg
      301 gcgaactcga accacgcaac ttctcgttca actccccaca tggcgcctgc ccggagtgca
      361 ccggtctcgg cacgacgatg gagttcgatc cggagctcgt gctgccgaac cgcaatctga
      421 cgatcgccca gggagcgatc gtgccctgga tgcggagtgg aatggaaaac tcgagctggt
      481 acgcctcact gatcgaggcc gtcgccgagc agtaccattt ctcgatccac gagccgatca
      541 aggatcttcc ggcggaggcg ctcggcatcc tgctctacgg cagcaacggc gaagccgtca
      601 ccgcccagta tgcgtcgaag aacgggcgca agcggagcta cagcgtcgtc ttcgaggggg
      661 tcatcccgaa cctgaagcgg cgctaccagc agacgcagtc cgactgggtg aaggaagaca
      721 tcgagcgcta catgtcgaca cggccctgcc cggtctgtca cggagcacgc ctcaagccgg
      781 aagtgctggc cgtcaccgtg aacgaggcat cgatcgtcga tgtcagccgg atgccgattc
      841 atgaagcgca gcagtggttc aatctcctcg gcgccgaacc aacggagcgc gacgcgattc
      901 tctcgaaccg cgagttcctg atcgcccgtc agatcctgaa ggagatccgg gaacggctcg
      961 gcttcctgat cgatgtcggg ctcaactacc tgacgctcga gcggtcggct aacacgcttt
     1021 ccggcggaga ggcccagcgc atccgcctcg cgacccagat cggctcgagt ctgatgggcg
     1081 tgctctacat cctcgacgag ccaagcatcg gccttcacca gcgcgacaac gcgaagctga
     1141 ttcgcacgct ggagcgcctg cgcaacatcg gaaacacgct gatcgtcgtc gagcacgacg
     1201 aggacacgat gcgcgccgcg gactggatcg tcgacatcgg accgggtgcc ggtgtgcacg
     1261 gtggttacgt cgtcgccgaa ggcacgatgg agcagattgc cgcgaccgag ggatcgatca
     1321 cgggagattt cctcagcggt cgccgttcga ttccggtccc ggccacacgt cgcaagggca
     1381 acggcaagga gatcgtgatt cggggcgcga aggagaacaa cctccgcgac gtcacggtgc
     1441 atttcccgct ggggacattc acctgcatca ccggcgtctc cggttccggc aagagctcgc
     1501 tgatcaccga cacgctctac aagcgtctcg cgcaggaact gcatcacgcc aaggacaagc
     1561 cgggctccca tgacgcgatg gaagggatca agcacctcga caaggtgatc gatatcaacc
     1621 agagtccgat cggacgcacg ccgcgctcga accccgcgac ctacacgggc ctcttcacgc
     1681 cgatccgcga gctctacgcc tccctcccgg aagcgaagac acgtggctac aaggcggggc
     1741 gtttctcctt caacgtgaag ggtggtcgct gcgaagcatg caagggtgaa ggcatcatcc
     1801 agatcgagat gaacttcctg cccgacgtct tcgttccctg cgaagtctgt cacggcgccc
     1861 ggtacaatcg ggaagcgctg gagatcctct acaagggcag ctcgatcgcc gacgtgctcg
     1921 acatgacggt tgcacaggcg ctcgacttct tcgagaacgt gccggcgctc aggaacaagc
     1981 tccagacgct cttcgacgtc ggtctcggct atgtgaagct gggacaaccg gcaacgcaac
     2041 tctccggtgg tgaggcgcag cgcgtgaagc tcgccagcga actctcgcgc cgcccgaccg
     2101 gcaggacgat gtacatcctc gacgagccga cgaccggact ccacttcgcg gacatcgaga
     2161 agctgctcgt gatcctccag cgtctcgctg atggcggaaa ctcggtgatg gtgatcgagc
     2221 acaacctcga cgtcatcaag tcggcggact ggatcatcga tctcgggccg gagggcggca
     2281 gcggcggcgg catgatcatc gcggagggca cgccggaagc gatcgcggag acaccccact
     2341 cgtacacggg agagttcttg cgtcccctcc ttccccagga agcgatcgcc gccgactgag
     2401 gcggtgagcc tgaacacagg
//
LOCUS       DASTMD010000842         1687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1509311, whole genome shotgun sequence.
ACCESSION   DASTMD010000842 DASTMD010000000
VERSION     DASTMD010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509311"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            725..877
                     /locus_tag="VFQ54_12475"
     CDS             725..877
                     /locus_tag="VFQ54_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165852.1"
                     /translation="MPKLVRLVAVAAVLTLVVRKKRDFRTQRQIMRSRLVQFPQRGGR
                     NQPVYV"
     gene            919..1608
                     /locus_tag="VFQ54_12480"
     CDS             919..1608
                     /locus_tag="VFQ54_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0165853.1"
                     /translation="MPSTTILTVEDESALVRLIRDYLVHEGFRAIAAQDARTALQRVQ
                     DDKPDLVILDLGLPDRNGFDVLREIRRTSDTPIIILTARGEETDRVVGLELGADDYMV
                     KPFSPKELVARIRAIMRRVDQRPTTSETLTAGDLVIDIPRMRITRGGQDIDLTATEFQ
                     IVAAMVREPGRVFTRGYLLDAIHGVIIESYERAIDAHIKNIRRKLEPDPAHPRYIVTV
                     HGVGYRFTDDR"
BASE COUNT          312 a          590 c          505 g          280 t
ORIGIN      
        1 tcgatttcca gctcatcaac ggtcaatggt tgatcgcgta tgtcaatcca acggtggact
       61 tctagggcga tcgcagtcga cgcatcgtgc caacgctcgc tttgcgaacg ccagcactgt
      121 cgtaggaagg tcgcacgtcc cacgacacga acgtccgcac ccgtggcggg tcaccaacga
      181 gcctgccctg gcagcggcgc cgagctcgtc gaagggtgtg acccatcggg cgcagcccga
      241 tatctccgtt ccatggatga cgcatggcca atcacgatct cccgtcccca cggcgcttgt
      301 cgccgaccca cgtccgggaa acgtcatgct cgatatgctg gtgtcgatgt ccggctgcgc
      361 cggatgtgct tcgcgggggg tccttcgcgt tgctcaggat gatgaggggg agaggttgcg
      421 gcgggagcac gtggatggca accgatccct cggcaagctg ctctgaaaag ctccgccctt
      481 cgggccctcc gacccaagct tttcatgccg cgctgccgag gcgagcgctt catgactact
      541 cgggatgacg gtggggtggg gttagccgcc gggcgcagga agcgcccgat cgtgcacgaa
      601 aactccgagc ccaattcaca tcctgttcac atctcgacgt cagaatagga agaagtcagg
      661 gaggcaatcg cccgatgccg ggcggctcct tccgcagata gccacaacca gagaggactc
      721 cctcatgccc aagctcgtcc gcctcgtcgc tgtcgccgcc gtccttaccc tcgtcgttcg
      781 caagaagcgc gacttccgca cccagcgcca gatcatgcgc tcgcgactcg tccagttccc
      841 gcagcgtggc ggtcgcaacc agccggtcta cgtctagttc ccagcccggg tccccagatc
      901 gccgcgaagg agcgcatcat gccgtccacg acgatcctca ccgtcgaaga tgagtcagcg
      961 ctcgttcgcc tgatccgaga ctacctcgtc cacgaggggt tccgcgcgat cgcggcgcag
     1021 gatgcacgga cggccctcca gcgagtgcag gacgacaagc ccgatcttgt catcctcgat
     1081 ctcggactgc ccgatcggaa cggcttcgac gtcctccgcg agattcgccg gacgtccgac
     1141 acacccatca tcatcctcac cgcccgcggc gaggagaccg atcgcgtcgt cggcctcgag
     1201 ctgggggccg acgactacat ggtcaaaccc ttcagcccga aggaactcgt cgcccggatt
     1261 cgcgccatca tgcgacgcgt cgaccagcgc ccgacgacct ccgagacgct cacggcgggg
     1321 gatctcgtga tcgacatccc ccggatgcgg atcacgcgcg gcggccagga catcgatctc
     1381 accgcaaccg agttccagat cgtcgccgcg atggtgcgcg aacccggacg tgtcttcacc
     1441 cgcggctacc tgctcgacgc gattcacggc gtgatcatcg aatcctacga gcgcgcgatc
     1501 gatgcccaca tcaagaacat ccggcgcaag ctcgagccgg atcccgccca cccgcgctat
     1561 atcgtgaccg tgcacggggt cggctaccgc ttcaccgatg accgctgaat cccaacgcac
     1621 gcccgggaat cgggaggatg gggaaccagt ggcgaaccgg gcgcgatacg gaccgccctg
     1681 ggcccag
//
LOCUS       DASTMD010000843         1886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2489463, whole genome shotgun sequence.
ACCESSION   DASTMD010000843 DASTMD010000000
VERSION     DASTMD010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2489463"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VFQ54_12485"
     CDS             <1..596
                     /locus_tag="VFQ54_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0165854.1"
                     /translation="YHELIFDGLRYTSLLSIPGALDRLIYCQSLSKTYAMTGWRIGYV
                     AARGALMKPIAQVARMLSGGVTWTVQRAAVAAINGPHDATRSMRDEYQARRDVITSLI
                     SETPELTWQLPEAAFYAFIRYEAPITASEAQALLGRNGVRARSGTEYGPSGEHHIRLS
                     FAASRESVIDGVLRVQAILGNNSEVLREYLAEKSRQA"
     gene            complement(593..1462)
                     /locus_tag="VFQ54_12490"
     CDS             complement(593..1462)
                     /locus_tag="VFQ54_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0165855.1"
                     /translation="MTATTEMRAPRMDEMDAIGQALLRWGAGDPLATGMQAGDFGWLQ
                     RNGAEWMTEKFLICVDARDEIVACGFREGDDGLWLQIDPARFFDVAVARAIVDGVEDA
                     GLREVSGPAAPSAVRAELARRGATIDPDPWPHLWRPLSDSDLIEAPGVFSTSSEALIA
                     ERIAVQRSAFANSTFTREKWATMAAGSLFRPELDLVALNDGGVGVSTLTAWLPESASC
                     GVIEPMGTHRDHWRQGHGGRVLRASFAALRRQGATGVRVGVERTNAPAISAYTSVGFR
                     VIGLDTTLVRQAG"
     gene            complement(1468..>1886)
                     /locus_tag="VFQ54_12495"
     CDS             complement(1468..>1886)
                     /locus_tag="VFQ54_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165856.1"
                     /translation="VGHLAAALGQLWTHTWITHVGRDELAIRASMRTGIQFLGNNFAT
                     AVYPSPDLDDVIPATHPWELAVVAIPDQMRLPVMQRLLDDLAANMAVGGHMVLGGDST
                     EVTRMLALVKKQPAFRERDRLKRRGASVAIVERREG"
BASE COUNT          331 a          688 c          555 g          312 t
ORIGIN      
        1 tctatcacga gctcatcttc gatgggctga gatacacatc cctgctctcg attccgggcg
       61 ccctcgatcg actcatctac tgccagagtc tctcgaagac gtatgcgatg acgggttggc
      121 gcatcggcta tgtcgctgcc cgtggcgcgc tgatgaagcc gatcgcccag gtcgcgcgga
      181 tgctcagtgg cggcgtgacg tggaccgtgc aacgcgccgc cgtcgcggcc atcaatggcc
      241 cgcacgacgc gacccgctcg atgcgcgatg agtatcaggc gcgccgcgat gtcatcacct
      301 cgctcatctc cgaaacgccg gagctcacct ggcagctccc ggaggcggcg ttctatgcct
      361 tcatccgcta cgaggcgccg atcaccgctt cggaggcgca ggcgttgctc gggcgcaacg
      421 gcgtccgcgc ccgctccgga accgaatacg gcccgtccgg cgagcaccac atccggctct
      481 cgttcgccgc gagtcgcgaa tcggtgatcg acggcgttct ccgggtccag gcgatactgg
      541 gcaacaatag cgaggtcctc cgcgagtatc tggcggagaa atcacggcag gcctaaccgg
      601 cctgccggac gagcgtcgtg tccagaccga tcacccggaa cccgacactc gtgtatgccg
      661 agatcgccgg cgcgttcgtc cgttccaccc ccacgcgcac gccggtcgcg ccctgccggc
      721 gcagcgccgc gaacgatgcc cgcaacacgc gccctccgtg cccttgtcgc caatgatcgc
      781 gatgggtccc cattggctcg atcacgccgc aggatgcaga ttccggcagc cacgccgtca
      841 gcgtcgagac gccgactcca ccatcgttca gggccaccag gtcgagctcc ggtcggaaga
      901 gtgatccagc ggccatcgtc gcccatttct cccgcgtaaa ggtcgaattc gcgaaggccg
      961 accgctgcac cgcgatccgc tccgcaatca gcgcctcgct gctcgtgctg aacaccccgg
     1021 gcgcctcgat caggtcggaa tcggagagcg gccgccacag atgcggccag ggatcgggat
     1081 cgatcgtcgc cccgcgacga gccagttcag cccgtaccgc tgacggcgcc gcgggaccgg
     1141 agacctcgcg cagacctgcg tcctcgacgc cgtcgacaat cgcccgcgcc actgccacat
     1201 cgaagaagcg cgccggatca atctgcaacc agagtccatc gtcgccttca cggaagccac
     1261 acgcgacgat ctcgtcgcga gcatcaacac agatgaggaa cttctccgtc atccactctg
     1321 cgccgttgcg ctggagccaa ccaaaatcac cggcctgcat cccagtggcg agcggatcgc
     1381 ccgcacccca tcgcaacagc gcctggccga tggcatccat ctcgtccata cgcggcgctc
     1441 gcatctcggt cgtagccgtc atgtccttta cccctcccgt cgctccacga tcgccacgct
     1501 tgctccccgg cgcttcaggc gatcccgctc ccgaaatgct ggctgcttct tgaccagtgc
     1561 aagcatccgg gtaacttccg tcgaatcgcc tccaaggacc atgtgtccgc ccacggccat
     1621 gttcgcggca agatcatcga gcagccgctg catgaccggc aggcgcatct gatccgggat
     1681 cgcgacgacc gccagctccc agggatgggt cgcgggaatc acgtcatcga gatcaggcga
     1741 tggatacacc gccgtcgcga agttgttgcc gaggaactgg attcccgttc gcatcgacgc
     1801 ccggatcgcc agttcgtcgc ggccgacgtg ggtgatccac gtatgcgtcc acaattgccc
     1861 tagcgccgcg gcgagatgcc cgacat
//
LOCUS       DASTMD010000844         7417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_256890, whole genome shotgun sequence.
ACCESSION   DASTMD010000844 DASTMD010000000
VERSION     DASTMD010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256890"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1271
                     /locus_tag="VFQ54_12500"
     CDS             183..1271
                     /locus_tag="VFQ54_12500"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: protein motif:HMM:TIGR02089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEU0165857.1"
                     /translation="MTEPMRIAAIAGDGIGTEVMPHGIAVVMAAARRFDIAIEFEEFD
                     FASADYYVRTGQMLPSDWFETLSRFDSIFFGAVGWPDVVPDHVSLWGSLLQFRRRFDQ
                     YVNLRPVRLMPGIPSPLVGREPGDIDFFVVRENTEGEYSSVGGIVFEGTDQEIVMQET
                     VMTRSGVDRILEYAYALAESRPARHLTSATKSNGISITMPWWDSRVEAIGLRHPGVRT
                     DKYHIDILTANFVLHPDRFDVVVGSNLFGDILSDLGPAVTGTIGIAPSGNINPERRYP
                     SLFEPVHGSAPDIAGTLRANPVGQMWSGAMMLDHLGHPEAAAAIVTAIETVVGNRGDL
                     SLLTPDIGGIGTTDSFGHAIVSALERPR"
     gene            1355..1651
                     /locus_tag="VFQ54_12505"
     CDS             1355..1651
                     /locus_tag="VFQ54_12505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEU0165858.1"
                     /translation="MTAVFHPREGATAELVEAFQGVIPPIHEEDGCLLYSIHAADDGT
                     ITMIEKWATQEDLDAHSVGEPVARLREAIAPYLSSPAVVTAMTPIPVGDSRGEV"
     gene            complement(1680..2450)
                     /locus_tag="VFQ54_12510"
     CDS             complement(1680..2450)
                     /locus_tag="VFQ54_12510"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0165859.1"
                     /translation="MSVSTGTILDGKIALVTAASRGIGRAISMDLARNGARVVLGVRD
                     VAKASGLVAEIESLGGSATAISMDVNELDACRQAIDDTVSRFGRLDILVNNAGGSIVD
                     DALDVQVSDFDAILALNVRSTFFISQYAAKHMANSGGGAVVNMASQAGLVALPGESVY
                     CLSKAAVIHMTKCFAVEWGAKGIRVNAVAPTFIQTDGTNAALSDDAFLADTVERIAAL
                     HRIGAPEEVASAVTFLASPGASLITGHTLVVDGGWTIR"
     gene            complement(2534..2962)
                     /locus_tag="VFQ54_12515"
     CDS             complement(2534..2962)
                     /locus_tag="VFQ54_12515"
                     /inference="COORDINATES: protein motif:HMM:NF016885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HEU0165860.1"
                     /translation="MLKNLNPLLSPDLLYALRAMGHLQDIAIVDANFPCAPSDRVIRL
                     DGVPALDVLSAVLSVLPLETQEPACAWRMVANGRPEQILPVFADFERVVDESEPGASI
                     TALDPADFKQLVGDSAAIVITGERRLYGSIVVRKGVIGPD"
     gene            complement(2994..3590)
                     /locus_tag="VFQ54_12520"
     CDS             complement(2994..3590)
                     /locus_tag="VFQ54_12520"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165861.1"
                     /translation="MVTSPIAIVILKVAAAIVIGGVVTFLFTLNRSQTPHVVISGVPW
                     VVPIVLVLLVAGTYVLSKTRYGRYIYAVGGNAEAARRAGIDVTKVRASVFIVSTSFAA
                     MGAVIYSSKVGSVDPTAGGLNTLLFSVGAAVIGGTSLFGGRGKLSYAVIGGLVVAVVE
                     NGLGLLNQPAAIVSIATGLVLALAAAVDALSRRRSVAS"
     gene            complement(3591..4427)
                     /locus_tag="VFQ54_12525"
     CDS             complement(3591..4427)
                     /locus_tag="VFQ54_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165862.1"
                     /translation="MSETAKAHPTGSSPRSTDSRADFAGDRGKQSTGRTVRGYAARLR
                     SGDLGSLPALLGLGVLVLTFAMTSPVFLSLGNISNLLAQGAGRILIAMGLVFVLLLGE
                     IDVSAGAASGVTAAVLALHYVNNGNLLGGMGTPVFVLFIAVNCIAIALAIWLRIWAAA
                     VLSLVPIAIVLIGFTANPWIEMLLAVTVGLSIGTITGFLVAKVRIPSFVITLALFLTW
                     QGVILQLIGQGGVLGIGTSQTLNAVANGNLSTLGSWLLYVIAVGGFSALTVIEYAFRT
                     RR"
     gene            complement(4447..5229)
                     /locus_tag="VFQ54_12530"
     CDS             complement(4447..5229)
                     /locus_tag="VFQ54_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0165863.1"
                     /translation="MVADILTLTAINKRFGPVQVLHDVSLSVRPGEVVALVGDNGAGK
                     STLVKCIAGTHAIDSGSVVFDGNEVHLNDPKSASKLGIEVVYQDLALADNLDIVQNMY
                     LGRESGSRWFMDEATMEEQARTTLSSLSVRTISSVRMPVSALSGGQRQIVAIAKSVLW
                     ESKVVLLDEPTAALGVAQTRHVLDLVRRLAEQGLGVVLISHNLADVFEVADRIAVLYL
                     GRLVAQVPTDTVTSNQVVELITSGRSGDLGLARSETAVTGAF"
     gene            complement(5229..6383)
                     /locus_tag="VFQ54_12535"
     CDS             complement(5229..6383)
                     /locus_tag="VFQ54_12535"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0165864.1"
                     /translation="MNKMINTLSRRGRIAVAVTAIGAAALMLSGCGANQTGSNNGTSS
                     GSGGSNGATKGTIGVILPSTADSARWESFDRPLLASALKADGYASNIQNAQGDAQSFS
                     SIADQMIASKVKALIITSPNPAVGASVELKAKAAGIPTIDYDRVNPGGAAGYYVSFDP
                     VEVGRLQGQAVADALKDKHGASVIQIEGDPTDINAVAFDKGAREALDPLYKSGALTLV
                     QDKFVLGWDNQLAGTDFEQVLTGNHGKVDGVVVANDGMAQAVITVLQKNGLNGKVAVT
                     GQDATLAALQSILRGDQTMTVFKEIKRQADAAAELAADAAAGDTAKANKFASATIKDP
                     KTGRVIKAVLLGSTNVNKSNVKEVVDKGYVTAKQLCSADLAKACAAAGIK"
     gene            7041..>7417
                     /locus_tag="VFQ54_12540"
     CDS             7041..>7417
                     /locus_tag="VFQ54_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU0165865.1"
                     /translation="MPTSATIRVVNQARVLAALRRQGPHSRVSLSEDLGLTRSTVTVI
                     VTTLLEQGLVEEVVSDSHDPRATGRPRVELRLRSGGAYFGGLDIGNETLTTVIVDLNG
                     HQVEHSSRPLRPGSSAIEAEHTL"
BASE COUNT         1423 a         2422 c         2262 g         1310 t
ORIGIN      
        1 aatcgggccg gtcgtcgtct ccctcgtcac cgcgatctca tcgcttggca tcagcatcct
       61 ggtctccgga gcgatcggcc tcgccgccgc cggcgcattg gctcgctgga cgccacgaag
      121 agccaagccg cccgatgcgg accctcaagc cctgtccacg accggatgac agaaggagca
      181 ccatgaccga accaatgcga atcgcggcca tcgcgggaga cgggataggc acagaggtca
      241 tgccgcacgg aattgcggtg gtcatggccg ctgcgagacg attcgacatc gccatcgagt
      301 tcgaggaatt cgacttcgcc agtgccgact attacgttcg gacaggccag atgctgccgt
      361 ccgactggtt cgagactctg tctcggttcg actcgatctt cttcggagcc gtcggttggc
      421 cggacgtcgt gcctgaccac gtctcgctgt ggggaagcct gctccagttc cgcaggcgct
      481 tcgaccagta cgtcaacctt cgcccggtgc gactcatgcc agggatcccg agccctctcg
      541 tgggacgaga gccgggagac atcgacttct tcgtggtgcg ggagaacacc gagggcgagt
      601 actccagcgt cggcggaatc gtgttcgagg gcaccgacca ggagatcgtc atgcaggaga
      661 cagtcatgac gcggtcggga gtcgaccgca tcctcgagta cgcatatgcc ctcgccgagt
      721 cgcgacccgc acgccacctc acgagcgcga ccaagagcaa cggcatctcg atcaccatgc
      781 catggtggga ctcacgggtc gaagccatcg gccttcgcca tcctggagtg cgcaccgaca
      841 agtaccacat cgatatcctc acggcgaact tcgtgctcca tcctgaccgg ttcgacgtcg
      901 tcgtcggcag caacctgttc ggggacatcc tgtccgacct cggacccgcc gtcaccggaa
      961 ccatcggcat cgcacccagc ggcaacatca atcccgaacg caggtatccg agcctcttcg
     1021 agcccgtcca cggatcggcg cccgacatcg cgggcacact acgcgccaat cccgtcgggc
     1081 agatgtggtc aggagcgatg atgctcgacc acctcggcca cccagaggcc gctgccgcta
     1141 tcgtcaccgc gatcgagacc gtcgtaggga accgcggcga tctgtcactg ctgactcccg
     1201 acatcggcgg catcgggaca acagactcgt tcggtcacgc catcgtctcc gcgctcgagc
     1261 gacctcggta aaatggtgag cgacgcaccc gagagcaggg cattgcccga ggtgacgaga
     1321 gatcaccgga gagagaacag aaatgaccgt catcgtgaca gcagtcttcc atcctcggga
     1381 gggcgcaacg gccgagctcg tcgaagcgtt ccagggggtc attccgccca tccacgagga
     1441 ggacggatgt cttctctact cgattcacgc ggcggacgac ggaaccatca ccatgatcga
     1501 gaagtgggcg acgcaggaag acctcgacgc gcactcggtc ggcgagcccg tggcccggtt
     1561 gcgtgaagcg atagcaccgt atctctcttc gcccgcggtg gtgaccgcca tgactcccat
     1621 cccggtcggc gactcgaggg gcgaggtcta gacccaccgc gaccgaggtc tcgccccgcc
     1681 tagcgaatcg tccacccacc gtcgacgacg agggtgtggc ccgtgatcag agaagctccg
     1741 ggggaagcca gaaaagtgac cgcagacgcc acctcttctg gcgcaccgat gcgatggaga
     1801 gccgcgatcc gctcgaccgt gtcggccaag aaggcgtcgt cggagagtgc ggcattcgtg
     1861 ccatccgtct gaatgaacgt cggggcgacc gcattgacgc gaattccttt cgcgccccac
     1921 tccaccgcga agcacttcgt catgtggatc accgcggcct tgctcaagca gtagacagac
     1981 tcgccaggca gagcgacaag acccgcttgc gacgccatgt tcaccactgc gccacctccg
     2041 ctgttcgcca tatgcttcgc cgcgtattga ctgatgaaga aggtggagcg cacgttgagg
     2101 gccaagatcg cgtcgaagtc gctcacctgg acgtcgagcg catcatccac aatgcttcca
     2161 ccggcgttgt tgacgaggat gtccaaccgt ccgaatcggg acaccgtgtc gtcgatcgcc
     2221 tggcggcaag cgtccagctc attcacatcc atcgagatcg ccgtcgccga tccgcccagc
     2281 gattcgatct cggcgacgag tccggacgcc ttggcgacgt ccctgacccc gaggaccaca
     2341 cgggcgccgt tcctcgccag atccatcgag atcgcgcgcc cgataccgcg agacgctgcg
     2401 gtcaccaacg cgatcttgcc gtccagtatc gttcccgtac tcacactcat gcgcccaacc
     2461 tccgaactcg acccgcgatg cctaccttgt gccccgccct agggccatct tgtcgtcaag
     2521 gagcacgaat gcgtcaatcc ggaccgatga cacccttccg gacgacgatg ctcccgtaga
     2581 gccgcctctc acccgtgatc acgatcgcgg cgctgtcgcc gaccagctgc ttgaagtcgg
     2641 ccggatcgag agccgtgatg gaggctccgg gttcggattc gtccacgacg cgctcgaaat
     2701 ccgcgaacac gggcaatatc tgctccggtc ggccattggc gaccatccgc caagcacagg
     2761 cgggttcttg cgtctcgagc ggaaggacgg agagcaccgc gctgaggaca tcgagcgcag
     2821 gcaccccgtc caagcggatg accctgtcgg acggcgcaca gggaaagttc gcatccacga
     2881 tggcgatgtc ctgaagatgt cccatcgccc ggagggcata gagcaggtcc ggcgagagca
     2941 gggggttcag atttttcagc atttctcggt ttcctcgaat tcgatcgacg gggtcaggaa
     3001 gcaacggaac gtcgacgcga gagcgcatcc acggcggcgg cgagcgccag aacgagtcca
     3061 gtcgcgatcg acacgatcgc ggccggttgg ttgaggaggc ccagtccgtt ctcgacgacg
     3121 gcgaccacga gccccccgat gacggcgtac gagagcttgc cccgtcctcc gaagagcgat
     3181 gttcctccga tgaccgcggc cccgacggag aaaagcaggg tgttgagacc accggctgtc
     3241 ggatcgacgg atcccacttt ggaggagtag atcaccgcgc ccatcgccgc gaacgaggtc
     3301 gacacgatga acacgctggc acgaaccttc gtgacgtcga ttcccgcgcg ccgagctgct
     3361 tcggcgttgc cgccgaccgc atagatgtaa cgcccatatc tcgtcttgct cagcacatag
     3421 gtgccggcga cgagcagaac gagcacgatg ggaacgaccc acggcacgcc ggagatcacg
     3481 acatggggag tctgcgaacg attcagcgtg aacaagaacg tgacgacgcc gccgatgacg
     3541 atcgcggcgg cgaccttgag gatcacgatc gcgatgggcg aggtgaccat tcaccggcgc
     3601 gttcgaaacg cgtactcgat gacggtgagc gcgctgaatc cgcccacggc gatgacgtac
     3661 aggagccaac tgccaagagt tgagagattg ccgttcgcaa cagcattcag cgtctgcgac
     3721 gtgccgatgc cgagcacccc gccctggccg atcagttgaa gaatcacccc ttgccaggtc
     3781 aggaacaacg ccagcgtgat gacgaaggac ggaatcctga ccttcgccac aaggaatccg
     3841 gtgatcgtgc cgatgctcaa tcccaccgtc accgcgagca gcatctcgat ccaaggattt
     3901 gccgtgaagc cgatcaagac gatcgctatg ggaaccaggc tgagaactgc cgcggcccag
     3961 atcctgagcc agatcgccag cgcgatcgcg atgcagttca cggcgatgaa gaggacgaat
     4021 accggggtcc ccattccgcc gagcaggttg ccgttgttca cgtagtgcag ggcaaggacc
     4081 gcggccgtca ctccggacgc cgcgccggct gagacatcga tctcgccgag caacaggacg
     4141 aagaccaggc ccatggcgat gaggatccgg ccggcgccct gggccaggag gttcgagatg
     4201 ttcccgagcg acagaaacac gggtgaggtc atggcgaacg tgagcacgag cacaccaagc
     4261 ccgagcagcg caggcagaga gccgagatca cctgatctca gccttgccgc atatccgcgc
     4321 accgtccggc cagtggattg cttccccctg tcgccggcga agtccgctct cgaatcggtc
     4381 gaccgcggtg agctgccggt cgggtgagcc tttgctgttt cagacatggt gatggaattc
     4441 cctaacttag aacgcgccgg tgaccgcggt ctcggagcgc gccagcccga ggtcgccaga
     4501 gcgcccagag gtgatcaact caacgacctg gttggatgtg acggtgtccg tgggaacctg
     4561 cgccaccagg cgtccgagat agaggaccgc gatacggtcc gcgacctcga agacgtcggc
     4621 aaggttgtga ctgatcagga caacgcccaa tccctgctcg gccagtcgac gcacaaggtc
     4681 caggacgtgt cgggtctgcg ccacgcccaa ggcggcggtc ggctcatcga ggagaacgac
     4741 cttcgattcc cagagaacgg acttcgcgat cgcgacgatc tgacgttggc cgcctgacaa
     4801 cgccgacacc ggcatccgaa ccgagctgat cgtcctgacg gacagggagc tgagcgtggt
     4861 gcgcgcctgc tcctccatgg tcgcttcatc catgaaccac ctcgacccgg attcccgacc
     4921 gagatacatg ttctgcacga tgtccagatt gtcggcgagg gcaagatcct gatacacgac
     4981 ctctattccg agcttcgaag cagacttggg atcgttgaga tgcacctcgt tgccgtcgaa
     5041 gacgacggaa cccgagtcga tcgcgtgagt tcccgcgatg cacttgacca gcgtggactt
     5101 accggctcca ttgtctccga ccagcgcgac cacctcgccg gggcgaacgg acaggctcac
     5161 gtcatgcaac acctgcaccg gcccaaagcg cttgttgatg gccgtgaggg tcaggatgtc
     5221 ggcaaccatc acttgattcc ggccgcagcg caggccttcg cgagatccgc cgagcacagc
     5281 tgcttggccg tcacgtatcc cttgtcgacc acctctttga cattggactt gttcacattg
     5341 gtcgacccga ggaggacggc cttgatgaca cggcctgtct tcgggtcctt gatggtcgcg
     5401 ctcgcgaact tgttggcttt cgcggtgtca cccgccgcgg cgtccgcagc gagttccgcg
     5461 gcggcgtcgg cctgacgctt gatctccttg aacaccgtca tcgtctggtc tccgcgcagg
     5521 atcgactgca acgccgccaa ggtggcgtcc tgccctgtca cggccacctt cccgttcaac
     5581 ccgttcttct ggaggaccgt gatgacggcc tgagccatcc cgtcattcgc gacgacgact
     5641 ccgtcgacct tgccatggtt gccggtgaga acctgttcga agtcggtccc ggcgagctgg
     5701 ttgtcccagc cgaggacgaa cttgtcttgt acgagggtga gcgccccgga cttgtaaagg
     5761 ggatcgagtg cttctctcgc ccccttgtcg aatgccacgg cgttgatatc cgtgggatcg
     5821 ccctcgatct ggatgactga cgcaccatgt ttgtccttga gcgcgtccgc gaccgcctgc
     5881 ccctggagcc ttccgacctc caccggatcg aacgacacgt aatatccagc ggcgccgccc
     5941 ggattgaccc ggtcatagtc gatcgtcggg attcctgccg ctttggcctt gagctcgacg
     6001 ctggcgccga cggcgggatt cggcgaggtg atgatcagcg ccttcacctt gctcgcgatc
     6061 atctggtcgg cgatgctcga gaagctctgc gcgtcgccct gcgcgttctg gatgttggag
     6121 gcgtagccgt ccgctttcag cgcgctcgcc aggagaggcc tatcgaacga ctcccacctc
     6181 gcagagtcgg cggtcgaggg aaggatcacc ccgatcgttc ccttcgtcgc tccgttcgac
     6241 ccgccggagc ccgaggacgt cccgttgttc gatccggttt gattcgcacc gcacccgctc
     6301 aacatgaggg cggcggcgcc gattgcggtg acggctacgg cgatgcggcc acgtcggctg
     6361 agggtgttga tcatcttgtt cattgctggt tctcttctct gagttggcaa tcggctgtga
     6421 aatagggcat tgcggggtca tcgggtcgga atcaaacgcg caccacggca ttcggcggga
     6481 caaggttctg gaaaagtccg aaccgtacgc tggtgggcgg ttgtcatgga gcacaggagc
     6541 gttgtgatgg gcctacgctc cggatcgtga gttctggcta cgctcgaggg ccgttccgcg
     6601 ggccctcgag agagatgtcg atgcttgatc gagccacgcc aaacgcctca cacgcggaaa
     6661 tgcgaccttg tccacgactg ccgctctgtc ggtgggaacc ggtggcaaga ccagacgtga
     6721 tcgacgacac aagtgccgaa cgttttggca tccgtcctcc tcgttgagtt tgttcgcgtc
     6781 tcggttgact ttgttcgcgt ctcgaattga cttcacttta tgccgtgtcc catggcccgt
     6841 caaggggcaa tctcgcgccc tccgccgccg cgcgcagcag ccgattccgc gccagaagcc
     6901 caacgcgcga aacgagaggg cgccacgcgc acgccggtcc gcccgttcgc gcaatggtgc
     6961 gaaaccgctt gacgcttcgc accgatcgtg cgtactgtag ctattattcg cggcacgaat
     7021 aaactcggtg aggagggctg atgccaacaa gcgcaacgat tcgcgtcgtg aatcaggcac
     7081 gcgtcctcgc cgccctgcga cgtcagggac cccactccag agtgagtctg tctgaggacc
     7141 tcggcctcac ccgttcgaca gtcaccgtga tcgtcacgac tctgctcgaa caaggcctcg
     7201 tagaagaggt cgtctccgac tcacacgatc cgcgggcaac cgggcgtcct cgagtggagc
     7261 tccgcctccg tagcggcgga gcctacttcg gcggtctcga catcggcaac gagacgttga
     7321 caacagtcat cgtggatctg aacggacatc aggtcgaaca cagctcgcgc ccgcttcgac
     7381 ctggcagttc cgcgatcgag gcagagcaca ctctcat
//
LOCUS       DASTMD010000845         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1776368, whole genome shotgun sequence.
ACCESSION   DASTMD010000845 DASTMD010000000
VERSION     DASTMD010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776368"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VFQ54_12545"
     CDS             <1..646
                     /locus_tag="VFQ54_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165866.1"
                     /translation="VWDAGTTKLDIVNDLLAALNYRSLWVDMTGQFQATPYVVPANRP
                     PAYDLLPGIVKEFIDGPTSIYLDAWSRDRDIYGVPNKVIAIQSSDGDTDPLVGQWTNE
                     DPDSPFSYPSRGRWITTVLTGVQTPDFSGEDDPAAATVAFLDAVAQRSGIAASSTQST
                     IALKTIPVPVQLLDVVRFANDPAGIDGPYSITSLKLNAYATGLLEPSLQEVLAL"
     gene            643..816
                     /locus_tag="VFQ54_12550"
     CDS             643..816
                     /locus_tag="VFQ54_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165867.1"
                     /translation="MKWAKVTAISPLRIQLDGETDPLTFTPRSLVAGLAVDDRVLTEL
                     VDKRRVIVGRLGG"
     gene            819..>1681
                     /locus_tag="VFQ54_12555"
     CDS             819..>1681
                     /locus_tag="VFQ54_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165868.1"
                     /translation="MGYDPIDFVDQPNPLNGDSAAPKLNAENLAHMQTQYASAMTDSG
                     TATAAAIAAQHEADNAAYGSGIGRFDIRNYGAKVDGIELTDCAMTSGSAVLDSVSFTF
                     TADDIGKVVGVGGAGYQSPDYSTNGNDGKLISTIVSVSNGLATLADAAVNTVSGAVVA
                     FGTPDDAAIAAAHAAATAAGGGTVFFPVGRTIVTTPIVINNFVSWAGVHRDSSWVEVI
                     FSAPGTGPAVAGTTDWLSCAGRTASNPLTGVNVHDFGVEDRFHIRPGGYTAPLKPINI
                     YETKRCSIYNM"
BASE COUNT          322 a          533 c          508 g          318 t
ORIGIN      
        1 ggtgtgggat gcgggaacaa cgaagctcga catcgtcaac gatcttctag ccgccctgaa
       61 ctaccgatcc ctgtgggtcg acatgaccgg ccaattccag gcaaccccct atgtggtacc
      121 cgcgaaccgc ccgccggcct acgacctgct gccgggaatc gtgaaggaat tcatcgacgg
      181 accgacctcc atctatctcg acgcgtggtc ccgcgatcgg gacatctatg gggtgccgaa
      241 caaggtcatc gccatccaat cctctgacgg ggacacggat ccgctggtcg gacagtggac
      301 gaacgaagat ccggattcgc cgttctctta tccgtctcgc ggacggtgga tcacgacggt
      361 cctgacaggg gtgcagactc ccgacttctc cggagaagac gacccggctg cggcgacggt
      421 tgcgttcctc gatgcggtcg cacaacggtc cgggatcgcc gcgtcttcga cccaatcgac
      481 gatcgcgttg aagaccattc cggttccagt gcagcttctg gatgtggtcc ggttcgcgaa
      541 tgatccggcc gggatcgatg gtccctattc gatcacgtcg ctgaagctga acgcgtatgc
      601 gaccgggctc ctcgagccca gcctccagga ggtgcttgcg ctatgaagtg ggccaaagtc
      661 accgctattt caccgctgcg aatccaactt gatggcgaaa cggatccgct caccttcact
      721 ccccggtcac tcgtcgccgg acttgccgtc gatgaccgtg tactcaccga gctcgtcgat
      781 aaacgccgcg tcatcgtcgg ccgtctagga ggctaaccat gggctatgac ccgatcgatt
      841 tcgttgacca gccgaatcct ctgaatgggg atagtgctgc accgaagctg aatgctgaga
      901 acctcgccca catgcagacc cagtacgcgt ccgcgatgac ggattcgggc acggcaactg
      961 cggccgcgat cgcggcacag catgaggcag ataacgcggc atacgggtcg gggattggtc
     1021 ggttcgacat ccgcaattac ggggcgaagg tcgacgggat cgagttgacc gactgtgcga
     1081 tgacctccgg gtcggccgtg ctcgattcgg tgtcgttcac cttcactgcg gacgacatcg
     1141 gcaaagtagt cggtgtcgga ggtgcgggat accagagccc cgattacagc actaatggga
     1201 acgacggcaa gctcatctcg accatcgtca gcgtctccaa cggcctcgcg acactcgcgg
     1261 acgcagccgt gaacacggtg agcggtgcgg tagttgcgtt tggtactccc gatgatgcgg
     1321 ccattgccgc cgcgcatgcc gctgcgactg cggccggcgg cggcacggtg ttcttcccag
     1381 tcggccggac catcgtcacg acaccgatcg tcatcaacaa tttcgtctcc tgggcaggag
     1441 tgcatcgcga ctcatcgtgg gtcgaggtga tcttctcggc gcccggcact ggccccgccg
     1501 tcgcgggtac gacggactgg ctctcctgcg ctgggcgcac cgcatcgaac ccactgaccg
     1561 gcgtcaacgt gcatgacttc ggcgtggagg acaggttcca cattcgcccg ggcggctata
     1621 cggcacccct caagccgatc aacatttacg agacgaagcg ctgctcgatc tacaacatga
     1681 a
//
LOCUS       DASTMD010000846         1863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2489979, whole genome shotgun sequence.
ACCESSION   DASTMD010000846 DASTMD010000000
VERSION     DASTMD010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2489979"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..429
                     /locus_tag="VFQ54_12560"
     CDS             232..429
                     /locus_tag="VFQ54_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165869.1"
                     /translation="MGKLVYDITVLFDGFITGPENPLGDGGMRLFDELAPVRFELERI
                     DAIPANDVTHMRVRVINTDVG"
     gene            433..1167
                     /locus_tag="VFQ54_12565"
     CDS             433..1167
                     /locus_tag="VFQ54_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0165870.1"
                     /translation="MKSTLLVGMEEFMILRDARVDDLPILLSIFDEAFREDAHTQLQI
                     AWRGRDSMLDGMAIALRHWHSDPKYHLIVAEGLPTGTTLGWACWSLGREASSANQPQR
                     DAPDPVSESSIDALNEITSGDIARCQREVLRPGDKVCILVSVAVSPQAQKHGVGRLLV
                     GWGTDYADAHDQRSWVHASEVGSRAFERLGFRRHRELRIDLDRYSPPQSERQWGPAIF
                     RSMVRPPVQAVLDSHQRPANTPESSS"
     gene            complement(1279..>1863)
                     /locus_tag="VFQ54_12570"
     CDS             complement(1279..>1863)
                     /locus_tag="VFQ54_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU0165871.1"
                     /translation="VTEKDIWTSALATAQFLSSQRPNGSAFAIGESGLTTALHEVGYA
                     LVDRNPDYVVIGETRTYSFERLTRAIRLISDGARFIATNPDPTGPSQEGPLPATGAVV
                     ALISKATGAQPYFVGKPNPLMMRSALRTLNVHSEETVMIGDRMDTDIVAGIEAGLRTV
                     LVLTGSTSREGAMRFPYLPTRIVDSIADLIDDVT"
BASE COUNT          356 a          564 c          596 g          347 t
ORIGIN      
        1 ctgtcgggca aatcactttc agccgcttga ggtgatgatg gatgagacat cacgaccggt
       61 acgacggtgc tgctgcgcca tcgagctgtg ggtgtggcga gagttgctcg tgtggtgagg
      121 ccggctagtt ctcgtcgaaa cgcgaggcat tgggcaagcg tggaggcgat cccgggatcg
      181 tctccacgcc gttgtgcccg ggtcaccacc agccagacca gagggagctt catgggcaag
      241 ctcgtctacg acatcacggt tttgttcgac ggtttcatca cgggaccgga gaatccgctc
      301 ggcgatggcg gaatgcgact cttcgacgag ctcgcgccgg tccggttcga gctggaacga
      361 atcgacgcga ttccggccaa cgatgtcaca cacatgcgcg tccgcgtcat caatacggat
      421 gtaggctagg cgatgaagtc gacgctcctc gtcggcatgg aggagttcat gatactcagg
      481 gacgcgcgcg tcgatgacct gccgatactg ctcagtattt tcgacgaggc atttcgggaa
      541 gatgcccaca cccagctaca gatagcctgg cgtggacgtg actccatgct cgacggtatg
      601 gcgatcgccc tgcgtcactg gcattccgac ccgaagtatc atctcatcgt tgcagagggc
      661 ctgccgaccg ggaccaccct tggttgggca tgctggtctc tcggccggga agcctcgtca
      721 gccaaccagc cccagcgtga cgctccggac ccggtttccg aatcctcaat cgatgcattg
      781 aacgagatca cgagcggcga cattgcgcgc tgccagcgcg aggttctgcg cccaggggac
      841 aaggtgtgca tcctcgtgtc cgtcgcggtt tcaccgcaag cgcaaaagca tggcgttggc
      901 aggttgctgg ttggctgggg aacggattac gccgatgctc acgaccaacg aagctgggtc
      961 catgcgtcgg aggtgggttc gagagcattc gagagacttg gcttcagacg gcatcgagaa
     1021 ctcaggatcg acctcgatcg ctactcgcca ccgcagagcg aacgacaatg gggacctgcg
     1081 atctttcgct cgatggtgcg tccgcccgta caggccgtgc tcgacagtca tcaaagaccg
     1141 gccaatacac ccgagagttc ttcatagcca gcgttggcca cgcgttggac ttgaccaatg
     1201 gtggaacgcg acgtcgcccg gacgacttgc gtccgttggc tcccaaaagt gcatggcagt
     1261 acccaggaaa ccaacacact acgtcacatc atcgatcagg tcggcgatcg agtcgacgat
     1321 ccgcgtcggc aggtagggga agcgcatcgc gccctcgcgt gacgtgctgc cggtcaggac
     1381 gagcaccgta cgcaagcccg cctcgattcc ggcgacgata tccgtatcca tccgatcgcc
     1441 gatcatcacg gtctcctcgg aatggacgtt caacgtccgc agcgccgacc gcatcatgag
     1501 gggattcggc ttgccgacga agtagggctg ggcgccggtc gccttcgaga tgagtgcgac
     1561 gacggcgccg gtcgccggga gcggcccctc ctgggatgga ccggtcggat cgggattcgt
     1621 cgcgatgaag cgcgcaccat cgctgatgag gcggatcgcc cgcgtcaggc gctcgaaact
     1681 gtaggtccgc gtctccccga tcacgacata atccggattg cgatcgacca gggcatagcc
     1741 gacctcgtgc agggcggttg tcaggccaga ctcgccgatc gcgaaggcgc tgccgttcgg
     1801 tcgctggctg gagaggaatt gcgccgtcgc cagcgccgag gtccagatgt ccttctcggt
     1861 gac
//
LOCUS       DASTMD010000847         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1510002, whole genome shotgun sequence.
ACCESSION   DASTMD010000847 DASTMD010000000
VERSION     DASTMD010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510002"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="VFQ54_12575"
     CDS             <1..1230
                     /locus_tag="VFQ54_12575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU0165872.1"
                     /translation="ACAIALHTPVGTIVDTGDFKFDPSPVMGGQTDERRLKRIGDEGV
                     LALFSDTVRVETDGSTPSEKVVMDTMAEVIGKAKGQVVIATFASNVSRIYMALNAAAK
                     HGRKVAVAGRSMEQNARVSIDLGYLDPPDGLLVPLDDLLKLPKERRVIVGTGSQGEAA
                     AALARIAAGEHPKIRVAKGDVILVSASPIPGNEDTISQTIDNLFRRGCDVVYSAINRG
                     VHVSGHAGRDELQKMIQLVRPKFAVPIHGEYRHMALYRDLCEANDIRRDHVLLPEIGG
                     VIEFTKDSAQQKGRVRAGNVLVDRLGDRDDSQVVLRTRENLTAEGVVVVTIVLDRQTG
                     ELIAGPELVGKGLKPELNTGALREAERELRRTLERRRKGEPQYGYVVQRTKETVGRAL
                     SRRSRTRPLILPVVTEL"
BASE COUNT          265 a          494 c          527 g          260 t
ORIGIN      
        1 gcctgcgcga tcgcgctgca cacgccggtc gggacgatcg tcgacacggg tgacttcaag
       61 ttcgacccga gccccgtcat gggcggccag accgatgagc gacgcctgaa gcgcatcggt
      121 gacgagggcg ttcttgccct cttctcagac acggtccgtg tcgagaccga tggcagcacg
      181 ccctcggaaa aggtcgtgat ggacacgatg gcggaggtga tcggcaaggc caagggccag
      241 gtcgtgatcg cgacgttcgc gtcgaacgtc agccgcatct acatggcgct caatgctgcc
      301 gccaagcatg gtcgcaaggt cgcagtcgcc gggcggagca tggagcagaa cgcccgggtt
      361 tcgatcgatc tcggttatct cgatcctccg gacgggctgc tcgtcccgct cgacgatctc
      421 ctgaagcttc cgaaggaacg ccgggtgatc gtgggtaccg gaagccaggg cgaggcggcg
      481 gccgcgttgg cccgcatcgc cgccggcgag catccgaaga tccgggtggc caagggcgac
      541 gtcattctcg tttccgcgag tccgatcccg ggcaatgagg atacgatcag ccagacgatc
      601 gacaatctct tccgccgtgg ctgcgacgtc gtgtacagcg cgattaatcg cggcgttcac
      661 gtctcgggtc acgccgggcg cgatgagctc cagaagatga ttcaactcgt gcggccgaag
      721 ttcgcggtgc cgatccacgg cgagtatcgc catatggcgc tctaccgcga cctctgcgag
      781 gcgaatgata tccgccgcga tcacgtgctc ctgccggaga tcggtggtgt gatcgagttc
      841 acgaaggaca gcgcccagca gaagggacgc gtgcgcgccg ggaacgtgct cgtcgaccgc
      901 ctgggggatc gcgacgattc ccaggtcgtc ctgcggacac gggagaacct gacggcggag
      961 ggcgtcgtcg tcgtcacgat cgtgctcgac cggcaaaccg gcgagttgat cgccggtcct
     1021 gaactcgtcg gcaaggggct caagccggag ctgaacaccg gcgcgttgcg tgaggccgag
     1081 cgcgagctgc ggcgcacgct ggagcgccgg cgcaagggcg aaccgcagta tggctatgtc
     1141 gtgcagcgga cgaaggaaac ggttggccgc gcgctcagcc gccgctcgcg tacgcgtccg
     1201 ttgatcctcc ccgtcgtgac cgagctctag cgccaaaccg gcaacggtag cgacttcggg
     1261 cgcgccgtgg gcgggacaag cgtcccgccc atcgatgcgc tgctcccgtc ccgggaacga
     1321 aatacgcccc gatgattcga tcatcggggc gtatgcgttg cgtgcaggga tcagcttgcc
     1381 gcgagttcat attccttggc ggattccgcc gtctcgtcac atgagccgct aaggtcgatc
     1441 catgtgtccg cccacgcgct gacggcgtcg gtcacgctcc cgagagcgcg acccttctcc
     1501 gtcaggtggt actcgatgcg caccggcgtg tccgggtgga ccgtcc
//
LOCUS       DASTMD010000848         1684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_885118, whole genome shotgun sequence.
ACCESSION   DASTMD010000848 DASTMD010000000
VERSION     DASTMD010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885118"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1626)
                     /locus_tag="VFQ54_12580"
     CDS             complement(244..1626)
                     /locus_tag="VFQ54_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0165873.1"
                     /translation="MEEREGRFVGIANQIWENPEVALKESFAAKLQADDLAADGFTIT
                     FGLGDLPTAFKAEWTNGEGGPIIGFLGEYDALPGLSQERSDIQTPIVADGPGHGCGHN
                     LLGTAALAAASVVKAWLQATNTPGTVRYYGCPAEETGEGKSFMARAGAFDGLDLALTW
                     HPGSTTVAWEMSTLASSKLKYRFKGRTAHAAANPETGRSALDAVELMNVGVNFLREHM
                     PDRARIHYVITNGGGAPNVVPDDCEVWYFVRSPERHQVDELIERVNNIARGAAMMTET
                     QVEINFLAGSYNYLPNHVVTEHIADVLQELGPITFTDEEYDFAKTIAGAFPESLRESL
                     AKADDVPANRIGEGLITDVYPLSGAGEVMSGSTDVSDVSWNAPTAQIMTTCFVVGVPG
                     HSWAITATSGMSIGHKGMLHAAKAMAITAADAIANPEILAKAKAEFDAATAGRPFKTP
                     IPAHIMPRKP"
BASE COUNT          253 a          533 c          588 g          310 t
ORIGIN      
        1 ggagcccgcc gtcgactgcg ggtgtcgcgg ggcgcgttgc acgtacgtcg gggaggcgac
       61 cacatgggtc gccgggacga actgtcatgc gaggcgcgtc gcacgtacgt cgggttcgaa
      121 cgggcacaag gcccgtgggg acgggatctc gatgtcttgg gaaagcagag gcagcggttg
      181 cgagggtaag ggcggaccaa gcgtccaccc ttacggaatt gcgtacgttg gatgccgata
      241 gggttacggc ttgcggggca tgatgtgcgc cgggatcggc gtcttgaacg gacggcccgc
      301 tgtcgcggcg tcgaattccg ccttggcctt cgccaggatc tccgggttcg cgatcgcgtc
      361 cgccgcggtg atcgccatcg ccttcgccgc gtggagcatc cccttgtgcc cgatcgacat
      421 cccgctcgtc gccgtgattg cccagctgtg ccccgggacg ccgaccacga agcaggtcgt
      481 catgatctgc gccgtcggcg cgttccacga gacgtcggag acatcggtcg agccggacat
      541 cacctcaccg gcaccggaga gcgggtagac atcggtgatc agtccctcgc cgatgcggtt
      601 cgccggaacg tcgtccgcct tggcgaggct ttcgcgtagt gattccggga aagcgccggc
      661 gatcgtcttc gcgaagtcgt actcctcatc ggtgaaggtg atcggtccca gttcctgcag
      721 cacgtcggcg atgtgctcgg tgacaacgtg gttcggcagg tagttgtagc tgccggcgag
      781 gaagttgatc tcgacctgcg tctccgtcat catcgcggcg ccgcgggcaa tgttgttgac
      841 acgctcgatc agctcatcga cctggtgccg ctccggcgag cgcacgaagt accagacctc
      901 gcaatcgtcc ggaaccacgt tcggcgcgcc accgccgttg gtgatgacgt agtggatgcg
      961 tgcccggtcc ggcatgtgct cgcggaggaa gttgacgccg acgttcatca gttccacggc
     1021 gtcgagggcg gagcgccccg tctccgggtt cgccgcggcg tgggcagtgc ggcccttgaa
     1081 gcggtacttc agcttgctcg aggcgagagt gctcatttcc caggccacgg tcgtcgatcc
     1141 cgggtgccag gtcagggcga gatcgagccc atcgaaggcg ccggcgcgcg ccatgaagct
     1201 cttgccttcg ccggtctctt cggcgggaca cccgtagtag cgaacggttc ccggcgtgtt
     1261 cgtcgcctgc agccacgcct tgacgacgct tgccgccgcg agggctgccg tgccgaggag
     1321 gttgtggccg cagccgtggc cgggaccatc ggccacgatc ggcgtctgga tgtccgaccg
     1381 ttcctgcgag agtccgggaa gggcgtcgta ctcaccgagg aagccgatga tcgggccgcc
     1441 ctcgccgttc gtccattctg ccttgaacgc ggtcggaagg tcgccgaggc cgaaggtgat
     1501 cgtgaagcca tcggcggcga gatcatcggc ctggagcttc gcggcgaagg attccttcag
     1561 ggcgacctcc ggattctccc agatctggtt ggcgatgccg acgaaacggc cctcgcgctc
     1621 ctccagccag tcgacgacat tcgcgtgcgg tggtcatgcc gctggcgccg gtgcagcaac
     1681 tgct
//
LOCUS       DASTMD010000849         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2222889, whole genome shotgun sequence.
ACCESSION   DASTMD010000849 DASTMD010000000
VERSION     DASTMD010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222889"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFQ54_12585"
     CDS             complement(<1..598)
                     /locus_tag="VFQ54_12585"
                     /inference="COORDINATES: protein motif:HMM:NF021882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU0165874.1"
                     /translation="MDSATIAAIATPAGEGGIGVVRISGPDAAGIASQVFRQQRRSGV
                     RVVDLETVESHRLLYGTVVDPDTERPVDEVLLAWMAAPHTYTREDTVELSCHGGPLPL
                     RETLRVVLAAGARHAEPGEFTLRAFLNGRLDLTQAEAVLQVIGARTSESLRLAVSDLA
                     GDLTRRLAPANGALISLLAYLDASADFPDDEIDVPDHRD"
     gene            903..1337
                     /locus_tag="VFQ54_12590"
     CDS             903..1337
                     /locus_tag="VFQ54_12590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU0165875.1"
                     /translation="MARKLDRQGVFDFIVEKPRTGHLATVREDGRPHVATIWIAVDRE
                     REEIVFNTGVTSVKGKNLLRTGYAALAVDDDEPPFSFVTLEGPVTLSEDPAELRRWAA
                     IIGGRYMGVDQAEAFGERNGVPGEYVVRLRPTHMTGVADIAE"
     gene            complement(1346..1981)
                     /locus_tag="VFQ54_12595"
     CDS             complement(1346..1981)
                     /locus_tag="VFQ54_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165876.1"
                     /translation="MSVTPPSTSAPDPLVPPTPPDQPRLFPAPMSRPAAPPPVDITHE
                     PATIPEPDSRVTFPVPPVAAPTATPASPPEMAPKTVAPAAIAPQQGMPSDAPEEIAFA
                     PKPTAVPATKPQPQPTHSYQTSTDARRTGDRWAPSLPDRLDTKILPRMLPFLIILVVI
                     LANIGVMTAVIAIIAIAIGGIFLFGVLRSARATSRKTTHMPRDRRRFWDDF"
BASE COUNT          373 a          651 c          809 g          385 t
ORIGIN      
        1 cgtcgcggtg atccgggacg tcgatctcgt cgtccgggaa gtccgccgag gcgtcgaggt
       61 aggcgagaag gctgatcagc gcgccgttcg ccggggcgag acggcgggtg agatcgccgg
      121 cgagatcgga gacggcgagc cggagactct cggatgtgcg ggcgccgatc acctggagca
      181 ccgcctctgc ctgcgtgaga tcgaggcggc cgttgaggaa ggcgcgcagc gtgaactcgc
      241 ctggctcggc gtggcgggcg ccggcggcga ggacgacacg gagcgtctcc cggagcggca
      301 ggggaccgcc gtggcaggag agctcgaccg tatcctcccg cgtgtaggtg tggggagccg
      361 ccatccaggc gagcaggacc tcgtcaacgg gtctctcggt gtcgggatct acgaccgtgc
      421 cgtagagaag gcgatggctt tcgacggtct cgagatcgac gacgcggacg cccgatcgtc
      481 gttgctgacg gaagacctgg ctggcgattc ccgcggcatc gggaccgctg attcgaacga
      541 cgccgatccc gccttctccg gcgggggtgg cgatggcggc aatggtggcg ctatccatga
      601 ctggttcctg atcgagacat ctctgtgggc gcagggcgtt acgaccctgc ttcgcggctc
      661 ctgatgtgac gtagcatagc ctgattgacc gtttcctctc cgccctcttc ctggctcctc
      721 gcgatcgttc gcgggttttg ctcgttacat ggctatgtgc cagcgagtca ctgaacgagc
      781 aaaagaacgt tagcacgtta cgatcgatcg tgcgctgcca tcgtgatacg gtggtggcac
      841 gcaactagca tgtccggttt cgatccggga gtcgcgtgta acagggatgt gaggagcacg
      901 agatggcacg aaaactcgat cggcaaggcg tcttcgattt cattgttgag aagccacgca
      961 ccgggcatct ggcgacggtg cgcgaggatg ggcggccgca tgtcgcgacg atctggatcg
     1021 cggtcgaccg cgagcgggag gagatcgtct tcaacaccgg cgtcacctcg gtgaagggga
     1081 agaacctgtt gcgcaccggc tacgcggcgc tcgccgtcga tgacgacgag ccgcccttct
     1141 ccttcgtcac gctcgaggga ccggtgacac tgagcgagga tccggcggaa ctccgccgct
     1201 gggcggcgat catcggtggg cgatacatgg gtgtggacca ggcggaagcg ttcggggaac
     1261 gcaacggtgt tcccggcgag tacgtcgttc gcctgcggcc aacgcatatg accggcgtcg
     1321 ccgatatcgc cgaatagcga cgttcctaga agtcgtccca gaagcggcgg cgatcccgcg
     1381 gcatgtgggt cgtcttccgg ctggtcgcgc gggcggagcg gagcacaccg aagaggaaga
     1441 tgccgccgat cgcgatcgcg atgatggcaa tcaccgctgt catgaccccg atgttggcca
     1501 ggatcacgac caggatgatc aggaacggca gcatgcgggg caggatcttc gtgtccaggc
     1561 gatccggcag actcggcgcc caacggtcgc ccgttcgccg ggcgtccgtg gatgtctggt
     1621 aggaatgcgt cggttgcggt tgcggcttgg tcgcaggaac cgcggtcggc ttcggcgcga
     1681 aggcgatttc ctccggcgcg tcgctcggca tcccctgctg cggtgctatc gcggcgggag
     1741 cgaccgtctt cggtgccatc tctggcggtg acgccggggt tgcggttggt gcggccacgg
     1801 gaggaaccgg aaacgtgaca cgtgaatccg gttccgggat cgtcgcgggt tcgtgcgtga
     1861 tgtcgaccgg cggcggagcg gcgggtcggg acatcggcgc cgggaagagc cgtggctggt
     1921 ctggcggtgt cggcggcacg agcgggtcgg gcgcggacgt cgatggcggc gtcacgctca
     1981 tgggaacaga ttccgtcgcc atgggagagc ccgtttcgga acccgaagga gcctgatacg
     2041 cggccagtag cgcgccacat gccgggcaga tcacatcatc cggggagacg acggcgccac
     2101 aattcccgca atgaatgacg gtccgggcag attcggatgg cgaggatggt gcatgctcgc
     2161 tcatggccag gcgcaaatct cccatcgatc cagctgaaaa cgattaccat cgtccatg
//
LOCUS       DASTMD010000850         4351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1329663, whole genome shotgun sequence.
ACCESSION   DASTMD010000850 DASTMD010000000
VERSION     DASTMD010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329663"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VFQ54_12600"
     CDS             complement(<1..663)
                     /locus_tag="VFQ54_12600"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0165877.1"
                     /translation="MSGKPGNLTVLGGARMDRRRLMAFAGVAAVAASGLKATGAGAQD
                     TFKVAFIYVGPVGDLGWSYAHDQGRKYLEENVAGVETAYSENVPENGSDAERVFTDYA
                     QKGYKLIIGTSFGYMDAMVNVAKKFPDAQFIHMTGYKTAENLGTGFGKIEEPRYVSGL
                     IAGKMSASGKLGYVAANPIPEVIRGINAFTLGVRKSNPAATVQVVWTNTWYDPQKERQ
                     AAE"
     gene            complement(686..1105)
                     /locus_tag="VFQ54_12605"
     CDS             complement(686..1105)
                     /locus_tag="VFQ54_12605"
                     /inference="COORDINATES: protein motif:HMM:NF024307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEU0165878.1"
                     /translation="MLKITVGDLSFVAMYEHAAPKTVAAFMKILPFRQKLIQARWSGE
                     SGWVPLGDFQLGVGIENATSYPSPGEILFYPGGYSETEILFPYGRTNFASTLGQLAGN
                     HFLTIIEGRENLKEVGRRIVWEGAQDILFENVENDET"
     gene            1466..1858
                     /locus_tag="VFQ54_12610"
     CDS             1466..1858
                     /locus_tag="VFQ54_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165879.1"
                     /translation="MRNATTPAREYATDCLAWHVDAPSAASFLTGSLLNDAIYSAAAA
                     SGINLRRAENIATIPGLGVTARVDGKFVAIGIAPLFATLDIRIEGKALGALSEIRLAG
                     NDAILVGSWKGIDHVCAIANPSAVAKAA"
     gene            2018..2986
                     /locus_tag="VFQ54_12615"
     CDS             2018..2986
                     /locus_tag="VFQ54_12615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0165880.1"
                     /translation="MPELNQFQDYLVHEFVDDYNDGVMPRREMMMRIIHITGGVAAAA
                     TMLTSLGVKAADAGVERKQFRPAPTTMQSALSVAADDPRIKASDVSFESSGAMVGAYE
                     ARPAAPRATPAAATPVTAGALPLILVCHENRGLTDHIKDVTRRLATNGYVAVALDLVS
                     REGGTAAHDQSEVPSILSSGDPNRHVADFQAALDFYESQPGVDASRVGMIGFCFGGGI
                     TWRSATRIAALKAAIPYYGPPPPLTDVPNIQAAVLGVYSSDAGDFANGGRDELEAALK
                     AAGKTYEIKVYPGTEHAFNNDTGPRYNAEQAAVAWSDALAWFAKYV"
     gene            complement(3060..>4351)
                     /locus_tag="VFQ54_12620"
     CDS             complement(3060..>4351)
                     /locus_tag="VFQ54_12620"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU0165881.1"
                     /translation="LLPALLRLIGDWINFPRIDTMRRLKRQDQTREALFVEKRGSGLW
                     GHLATAVMRRPGRSIVLAGGLLVLAALPVLTMNVGQQSDSTLPKTDYSSGYAILSKDF
                     AAGMQSPIDFVIPGDVNDATTKDNVAKLVATLKTDSRLAEVTSRTSEDGQLTLVEAVP
                     VIDPSSKEAEHMIGDLREKTVPSLFGSQADDVYVGGNTAFTYDFDKVLSDRLPYVFAF
                     VLGLSFLLLLVAFRSIVVPLMSIVLNLLSVGAAYGTLVAVFQHGWGASLFGFNQVDVI
                     TNYLPVFIFCILFGLSMDYHVFLLSRIRERWDHTQDNAESIAFGVQSTGRIITGAALI
                     MVAVFSSFAMGRLVEIQQLGFGLGVAVLLDATLVRTILVPATMKVRGRANWWFPRFLH
                     WIPDPKIEGDLAPVVLKPRVERTLPPRPRTWEPVVSE"
BASE COUNT          814 a         1417 c         1321 g          799 t
ORIGIN      
        1 ctcggcggcc tgtcgttcct tctgcgggtc gtaccaggtg ttggtccaga cgacctgcac
       61 cgtcgcggcc gggttcgact tgcggacgcc gagggtgaag gcgttgatcc cgcggatgac
      121 ttccggaatc ggattcgcgg cgacgtagcc aagcttgccg ctggcggaca tcttgccggc
      181 gatcagcccg gagacgtaac gcggctcctc gatcttgccg aagccggtgc cgaggttttc
      241 cgccgtcttg tacccggtca tgtggatgaa ctgggcgtcc gggaacttct tcgcgacatt
      301 gaccatcgcg tccatgtagc cgaacgacgt gccgatgatc agcttgtagc ccttctgcgc
      361 gtagtccgtg aagacgcgct cggcatcgct cccattctcc ggaacgttct cggagtacgc
      421 ggtctcgacg ccggcgacat tctcctcgag gtacttgcgc ccctggtcgt gcgcgtagct
      481 ccagcccaga tcgcctaccg ggccgacgta gatgaacgca accttgaacg tatcctgtgc
      541 acccgctccc gtcgccttca atccggatgc cgccacggct gcgacaccgg caaacgccat
      601 caggcgacgg cgatccattc gcgccccacc cagaaccgtc agattgcccg gcttcccact
      661 catgatcttc tcccccaacg accggtcatg tttcgtcgtt ctccacgttc tcgaacagga
      721 tgtcctgggc cccttcccag acgattcgac gaccgacctc cttcaggttc tccctgcctt
      781 cgatgatggt caggaagtga ttgccggcga gctggccgag cgtgctcgcg aagttggttc
      841 ttccatatgg aaagaggatc tccgtctcgc tgtatccacc cgggtagaag agaatctcac
      901 ccggggaggg ataactcgtc gcgttctcga tccctacacc gagctggaaa tctcccagcg
      961 gcacccagcc cgactccccg ctccagcgcg cctggatcag cttctggcgg aagggaagga
     1021 ttttcataaa tgccgcgacg gttttcggcg ccgcatgctc gtacatcgcg acgaacgaca
     1081 aatcaccgac ggtaatcttc agcatggtcc cttgctcgat ccgttttgtc ttcacatttc
     1141 attcgatgtg cacgttcatt gaccctgaat ccgaattgtc tcacaacctc cgttccccga
     1201 gcagaccggg tcccgcgcac cccgccgggg tgtcggactc gtcgcaccac cgcacccgcg
     1261 gcgtttgtgc gcggcgaccg atcgttcttc tcccccgcca atctcccgca cctcgtgcat
     1321 atccgacgga gccggtctga ttttgagaat aattctcaat aggggtatac accgactgcc
     1381 gacgatgata gactaaggtc attgatgaga atgattctca ttactgatat ggtgaatcgg
     1441 atatgagttg atcggagttc gacggatgag aaacgctacg acaccagcac gtgaatatgc
     1501 gaccgactgc ctcgcctggc atgttgacgc gccatccgcg gcgtcgtttc tcacgggctc
     1561 gctcctgaat gatgcgatct acagcgccgc cgcggcgagc gggatcaacc ttcgccgcgc
     1621 cgagaacatc gcgacgattc cggggctcgg cgtgaccgcg cgggtcgacg gcaagttcgt
     1681 ggcgatcggg atcgccccgc tcttcgcgac gctcgacatc cggatcgagg gcaaggcgct
     1741 gggcgcactt tccgaaatcc gcctcgccgg caatgacgcg attctcgtcg gttcctggaa
     1801 gggcatcgat cacgtctgcg cgatcgccaa cccgtcggcg gttgcaaagg ctgcctagtt
     1861 ctccggaccc gacagggacg tacagcttca attctggaat acgtgaatag cttttcgtgg
     1921 atcgaatcgt gcggctgcgg gagggaggga tgtcgatgga gcatctttct ctcccgcttt
     1981 ttgtcgcccc gatccctttc caactggaga atgcctcatg ccggagttga accagtttca
     2041 ggactacctc gttcatgaat tcgtcgacga ctacaacgac ggcgtgatgc cgcgacgcga
     2101 gatgatgatg cgcatcatcc acatcaccgg gggcgtggcg gcggcggcca cgatgctgac
     2161 ctcgctcggc gtgaaggcag cggatgccgg cgtcgagcgc aagcagttcc gtcccgcccc
     2221 gacgacgatg cagagcgcgc tttccgtcgc cgccgacgat ccccggatca aggccagcga
     2281 tgtcagcttc gaatcgtccg gcgcgatggt cggtgcctac gaggcgcgcc ccgcggcacc
     2341 ccgcgccacg ccggcagcgg cgactccggt caccgcgggc gctcttcccc tcattctcgt
     2401 ctgccacgag aaccgcggac tcaccgatca catcaaggac gtgacccgcc ggctcgccac
     2461 gaatggatac gtggcggtgg cgctcgatct cgtcagtcgc gaagggggga ccgccgcgca
     2521 cgatcagagc gaggttccct cgatcctgag ttccggcgat cccaaccgcc acgtcgccga
     2581 tttccaggcc gcactcgact tctacgaaag ccagccgggc gtcgatgcct cccgcgtcgg
     2641 gatgatcggc ttctgtttcg gcggtggtat cacctggcga tcggcgacac gaatcgcggc
     2701 cctgaaagcg gcaatcccct attacggtcc gccaccgcca ctcacggatg tccccaacat
     2761 ccaggccgcg gttctcggcg tctactcctc ggatgccggc gacttcgcca acggcggccg
     2821 cgatgaactc gaggcggcgc tgaaggccgc cggcaagacg tacgagatca aggtctatcc
     2881 cggcaccgaa cacgccttca acaacgacac gggaccccgc tacaacgccg aacaggccgc
     2941 ggtcgcgtgg agcgacgccc tggcctggtt cgcgaagtac gtctagattc tggagacaag
     3001 gaccccgcgg tggacggctg gccaccgcgg ggtatttgcg ttgggaaagg aactgaggac
     3061 tactcgctga cgaccggctc ccaggtgcgg ggccgcggcg gaagcgtgcg ctccacacgc
     3121 ggcttgagga ccaccggggc gaggtctccc tcgatcttcg gatccgggat ccagtggagg
     3181 aagcgcggga accaccagtt ggcacgcccg cgcaccttca tcgtcgccgg cacgaggatc
     3241 gtccggacca gtgtcgcgtc gagcagcacc gccacaccga ggccaaaccc gagctgctgg
     3301 atctcgacca gacggcccat cgcgaaggag gagaagacgg cgaccatgat cagcgccgca
     3361 ccggtgatga tccggccggt cgactgcacg ccgaaggcga ttgactcggc attgtcctgc
     3421 gtgtgatccc atcgctcacg gattcggctg agcaggaaga cgtgatagtc catgctgaga
     3481 ccgaagagga tgcagaagat aaagaccgga aggtagttcg tgatcacgtc gacctggttg
     3541 aagccgaaga ggcttgcgcc ccatccgtgc tggaagaccg cgaccagcgt gccataggcc
     3601 gcaccgaccg agagcagatt gagcacgatc gacatcagcg gcacaacgat cgatcggaac
     3661 gcgaccagca ggagcaggaa gctcaagccg agcacgaagg cgaagacgta gggcaggcga
     3721 tcggagagca ccttgtcgaa gtcgtaggta aaggcggtgt tgccaccgac gtagacgtcg
     3781 tccgcctggc tgccgaacag cgacgggacg gtcttctcgc gcaggtcgcc gatcatatgc
     3841 tccgcctctt tcgaggacgg gtcgatcacc gggacggcct cgaccagcgt cagctggcca
     3901 tcctcactgg tgcgcgaagt cacttccgca agacgggagt ctgtcttcag ggtcgcgacc
     3961 agcttcgcca cgttgtcctt cgtcgtcgcg tcattcacat cgcccgggat cacgaagtcg
     4021 atcgggctct gcataccggc cgcgaaatcc ttcgacagga tcgcgtatcc gctcgagtaa
     4081 tcagtcttcg gcagcgtcga atccgactgc tgaccgacgt tcatggtcaa cacaggcagc
     4141 gcggccagga ccagcagccc accggcgagt acgatcgagc gaccgggacg tcgcatgacg
     4201 gcagtggcga gatgtcccca gaggccggat ccacgcttct cgacgaagag ggcttcgcgg
     4261 gtctggtcct gtcgcttcag gcgacgcatc gtgtcgattc gcgggaagtt gatccagtcc
     4321 ccgatcaggc gcaggagtgc gggcagcagc g
//
LOCUS       DASTMD010000851         4948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1510245, whole genome shotgun sequence.
ACCESSION   DASTMD010000851 DASTMD010000000
VERSION     DASTMD010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510245"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFQ54_12625"
     CDS             complement(<1..373)
                     /locus_tag="VFQ54_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0165882.1"
                     /translation="MDDGSITTPFGATTTAMEVVADVDLRGKRAIVTGGASGIGLETA
                     RALASAGAEVTIGVRNLEAGMKAAAGIAPAVGEGRVLVMPLDLADRAAIAAFVASWED
                     PLDILVNNAGIMASPEMRTAEG"
     gene            485..1042
                     /locus_tag="VFQ54_12630"
     CDS             485..1042
                     /locus_tag="VFQ54_12630"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEU0165883.1"
                     /translation="MVKERRPRADAQRNRDRLLEVAMHAFATEGPDVSLEAIARTAGV
                     GIGTLYRHFPTRDALVEAVYRNEISRVSALAGEFRASMAPETALRAWMTHFVAFMTTK
                     HGMSDVFRAVIASGGNPYGETRILALDAVQSLIAAGARAGTIRDDVSPLDILTILNGL
                     SFATDDPEQTARLIDLVIDGLRRRD"
     gene            1147..1899
                     /locus_tag="VFQ54_12635"
     CDS             1147..1899
                     /locus_tag="VFQ54_12635"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0165884.1"
                     /translation="MSGPDRDLKNRVAIVTGAARGIGYAIAARLVENGARVAIADVDV
                     AAANEAAARLGEHAIGVGLDVTSEDGWTAINEQLTRTWESVSILVNCAGIAGRAVPSW
                     ELSLKEWKQVIDIDLTGVFLGCHATIPGMLERGYGRIVNISSIAGKEGNPNATPYSAA
                     KAGVIGLTKAIAKEVATKGVIVNAVTPAVIETDILKQLTDEHVAYMTSRIPMGRVGQP
                     EEVANLVAFLCSDEITFSTGAVFDISGGRATY"
     gene            complement(1901..3454)
                     /locus_tag="VFQ54_12640"
     CDS             complement(1901..3454)
                     /locus_tag="VFQ54_12640"
                     /inference="COORDINATES: protein motif:HMM:NF023172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEU0165885.1"
                     /translation="MASVSAIRLRTALPLSATDARVAVVTVISVLVSFGATFALDQVA
                     DFGASSLVLAVVLTLTLGRSAQRNGLRERMLSFFILPAVAIVAGGVGGLMLHHAWIGD
                     ALFVVALSGAIWVRRFGTRWTRIGTIASLPFIAMLVAPAPVRADGSTNWVATAVVGMI
                     AFVTVNGVQFIAERIGYLASVERPRHEDASGSSRSATSRFAASTRMALQMAVSLGIAF
                     LAGHLLFGVHWPWMVLTVYIVQSGNRGRGDVVYKGIQRLAGALLGTLAATVIAGSFAP
                     GDRTAIVLIFAIIIIATFLRHVSYAWWAGGVTASLALLYGFFGESGDSLVETRLAAIV
                     LGAVLAVGVSWLLLPIRTTDVLRLRTAHVLATLSDFLGALRTDPASLAQQQERFSRAV
                     AELERLAPPLEAHHRWQRIRQSHSPFFVPVQAIRRSADAVERIVHLATVDPRQMERAE
                     TSRMTGLVAANVGLLRKSIRTRVTPTFRSTPEQSGTPLADALRDLDASLPALAAFLAP
                     NAPDEMTAA"
     gene            3662..4132
                     /locus_tag="VFQ54_12645"
     CDS             3662..4132
                     /locus_tag="VFQ54_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165886.1"
                     /translation="MVLFPWEWKRARLKREMAVPPKETFAGYRFALKGSPRHFDDVKA
                     IVEQSGTAKVYFGEALAYERGAGVALWRIRARDFDWLQPLYDWWVEMERIEPIQFTFH
                     LYLPDDPKYAVLDLRSTNPADVAAFIKERAPWEDNARGVTPTRLPAGQPFSAQH"
     gene            complement(4549..>4948)
                     /locus_tag="VFQ54_12650"
     CDS             complement(4549..>4948)
                     /locus_tag="VFQ54_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0165887.1"
                     /translation="SALVERIRLMCANVSPPQGAPDMVHADLKPDNVLVRDGVVVAVV
                     DIGNAGSGTRSTDLATLQWHTYEDRLDGARRRLWTRIIDLVGWEGAAVPTATQILTQL
                     EFPIRHGRHDDVPEVVERGHRALDELNALR"
BASE COUNT          918 a         1628 c         1608 g          794 t
ORIGIN      
        1 acccctccgc ggtgcgcatc tcgggtgacg ccatgattcc ggcattgttg accagaatat
       61 ccagcggatc ctcccaggag gctacgaagg ccgcgatcgc cgcccgatcg gcgagatcga
      121 gcggcatcac gagcacgcgt ccctccccca cggccggcgc gatccccgcg gccgccttca
      181 tccccgcctc caggtttcgc acgccaatcg tcacctcggc gccggccgat gccaacgcgc
      241 gtgccgtctc caggccaatt cccgaggcgc cgccggtgac gatcgcgcgc ttgcccctga
      301 ggtcgacatc cgcgacgacc tccatcgccg tcgtcgtcgc gccaaacggc gtcgtgatcg
      361 atccatcgtc catctgatac tgctccttgc ttgcacatct cgtacaatag aaaaacggag
      421 gttcctccac tacggaccac tttatacgga gcctcctccg tttacaagag cgacaggaga
      481 atacatggtg aaagagcgac ggccacgcgc cgacgcgcag cgtaatcgcg atcggctgct
      541 tgaggtggcg atgcatgcat ttgccacgga gggaccagac gtctcgctgg aagcgatcgc
      601 gcgcacggcc ggggtcggaa tcggcacgct ctacaggcat ttcccgaccc gtgatgcgct
      661 cgtggaggcg gtctatcgca acgagatcag ccgcgtcagc gcactcgcgg gggaattccg
      721 ggcgtccatg gctccggaga cggcgctccg tgcctggatg acgcacttcg tcgccttcat
      781 gacgaccaag cacggaatgt cggatgtctt tcgtgccgtg atcgcgtctg gcggaaatcc
      841 atacggcgag acccgtatcc tcgcgctcga cgcggtgcag tcactgatcg cggccggggc
      901 acgggcaggc acgattcgcg acgatgtctc cccgctcgac attctgacga tcctcaacgg
      961 cctctccttc gccaccgacg acccggagca gaccgcgcgt ctcatcgatc tcgtcatcga
     1021 cggacttcgc cggcgggact gagcacgcgg ccccagggct gtacgtgtgg tcagaacgag
     1081 ttccgctgtg taagatcgcc caagaacgtt ccaacaatga attgcccgct accctggaga
     1141 tttcgcatgt ccggaccgga tcgagacctg aagaatcgcg ttgccatcgt gacgggagcg
     1201 gcacgcggaa ttggctacgc catcgccgcg agactcgtcg agaacggcgc gcgggtcgcg
     1261 atcgccgacg tcgatgtcgc cgccgcgaac gaggcggcag cccggctcgg ggaacacgcg
     1321 atcggggtcg ggctggatgt caccagcgag gacggctgga ccgcgatcaa cgagcaactc
     1381 acccggacat gggagtcggt ttcgatcctc gtcaactgcg ccggcatcgc cggtcgcgcc
     1441 gtaccgtcgt gggagctctc gctcaaggaa tggaagcagg tgatcgatat cgatctcacc
     1501 ggcgtcttcc tcggctgcca cgccacgatt cccgggatgc tcgagcgcgg ctatggccgg
     1561 atcgtgaaca tctcctcgat cgccggcaag gaaggcaatc ccaacgcgac cccgtactcc
     1621 gcggcgaaag ccggcgtgat cggtctcacc aaggcgatcg ccaaggaagt cgcgacgaag
     1681 ggcgtgatcg tcaatgccgt gaccccggcc gtgatcgaga ccgacatcct caagcaactc
     1741 accgacgagc acgtcgccta catgacgagc cggatcccga tgggccgcgt cggccagccg
     1801 gaggaagtcg ccaacctcgt cgccttcctc tgctcggacg agatcacctt ctcgactggc
     1861 gccgttttcg acatctccgg gggccgcgcc acgtactagt tcacgcggcg gtcatctcgt
     1921 ccggcgcgtt cggggcgagg aatgccgcga gcgccggcaa cgatgcgtcg agatcccgga
     1981 gcgcatcggc gagcggcgtc ccggattgct ccggggtcga ccgaaacgtc ggcgtgacgc
     2041 gggtccggat cgacttccgc aggagcccca cattcgcggc gaccagaccg gtcatccggc
     2101 tcgtctccgc tcgctccatc tgccgtggat cgacggtcgc gagatgcacg atgcgctcga
     2161 ccgcgtcggc actgcggcga attgcctgga ccggcacgaa gaacggtgaa tgggattgcc
     2221 ggatccgttg ccatcgatga tgcgcttcga gcggcggagc cagtcgctcg agttccgcga
     2281 ccgcgcggct gaatcgttcc tgctgctgag ccagggacgc ggggtcggta cggagcgcgc
     2341 cgagaaaatc ggagagcgtc gccagcacat gggccgttcg cagacggagg acgtcggtcg
     2401 tcctgatcgg aagcaggagc cacgacacac caacggcgag gaccgcgccg aggacgatcg
     2461 cggcaagtcg cgtctcgacg agggaatcgc ctgactcccc aaagaagcca tagagcaagg
     2521 caagcgaggc cgtcactcca ccagcccacc aggcgtagct gacgtgccgc aggaacgtcg
     2581 cgatgatgat gatcgcgaag atcaggacga tcgccgtcct gtccccggga gcgaaggagc
     2641 cggcgatgac cgtcgcggcg agcgtgccca gcaacgcacc ggccaggcgc tggattccct
     2701 tgtagacgac atcgccgcga ccacgatttc cgctctggac gatgtagacc gtgagcacca
     2761 tccacggcca gtgcacgccg aagagcaggt gaccggcgag gaaggcgatc cccagcgaga
     2821 cggccatctg gagcgccatt cgcgtgctcg ccgcaaagcg cgaggtcgcg gatcgggagg
     2881 agcccgatgc gtcctcgtgg cgcggtcgct ccacggatgc gagatatccg atgcgctcgg
     2941 cgatgaactg gacgccattc acggtcacga aggcgatcat tcccaccaca gcggtcgcca
     3001 cccagttcgt cgatccatcg gcgcgtaccg gtgccggggc gacgagcatc gcgatgaacg
     3061 gcagcgatgc tatcgtgccg atcctcgtcc accgggtccc gaagcgacgc acccagatcg
     3121 caccactgag cgcgacgacg aagagcgcat cgccgatcca ggcgtggtga agcatcagcc
     3181 caccgacccc acccgcgacg attgcgaccg cgggaagaat gaagaaggag agcatgcgct
     3241 cgcggagccc gttccgctgc gccgagcgac cgagtgtcaa cgtgagcacg accgcgagga
     3301 caagcgaact cgcaccgaaa tccgcgacct gatcgagggc gaaggttgca ccaaaactca
     3361 cgagcacgga gatgacggtg acgaccgcga cgcgggcatc ggtcgccgat agcggaagag
     3421 cggtacgcag gcggatcgcg gaaacggacg ccatggtcgg gggatctcca ctcgcacggc
     3481 gttcgcaacg ctccggttac gtatagccgt cttcctgagt gtatgtgcgg gggatcagcc
     3541 gatcaagtgt tcgggaatgc gggcgcatcc gggcgttcgc ggtacaatca cgggcaagat
     3601 ggatcgacct ccgcgacggg gcggaacggc aggatccgat cggcaggagg caaggacgcg
     3661 gatggtcctc tttccatggg aatggaagcg cgcgcggctc aagcgtgaga tggcggtacc
     3721 gccgaaggag accttcgccg gctatcggtt cgcgctgaag ggttcgccgc gtcacttcga
     3781 tgatgtgaag gcgatcgtcg agcaatcggg cacggccaag gtctatttcg gcgaggcgct
     3841 tgcctacgag cgcggcgccg gcgttgcgct ctggcggatt cgcgccaggg atttcgactg
     3901 gctgcagccg ctctacgact ggtgggtcga gatggagcgg atcgagccga tccagttcac
     3961 cttccacctc tacctgcccg atgaccccaa atacgcggtc ctcgatctcc gcagcacgaa
     4021 cccggccgac gtcgccgcct tcatcaagga acgcgcgccg tgggaagaca acgctcgcgg
     4081 cgtcaccccg actcggcttc ccgccggaca gcccttctcc gcccagcact gattccgcac
     4141 gccaggcaag cgcagggctc cgatcgaatc cagtcgatcg gggccctggg cgtctgatgg
     4201 ggaggtggat tcgacgcgag cgaggcgtac cccctcgacg gaattcggcg aggcttttgc
     4261 caaaccgcct ggtggtgcgt ccacgaagtc gcgatagatg cagtcaaacg aggaccccgc
     4321 gtcggagcaa gcgtccgctc gtagggtggg acttctatgt cccaccttcc ggggcggcac
     4381 acgtcccgtc caggatttcg tggatggcat cgatggcatc gggacttgcc gacttcgggc
     4441 tccagcggtc tcggtggggt ctggtatggc ccgcgtggat attgcgcgtt atgccggctg
     4501 atagcagtca gtggtcgcca tgtcgcacac catctccacc ggcggcagtc agcgaagcgc
     4561 gttcagctcg tcgagggcgc gatggccgcg ctcgaccacc tctggaacgt catcgtggcg
     4621 cccgtgacgg atagggaact cgagttgcgt gagaatctgt gtcgctgtgg ggaccgccgc
     4681 tccctcccag ccaaccaggt caatgattct cgtccacagc cgccttcggg caccgtccag
     4741 ccgatcctcg taggtgtgcc actggagggt agcgagatca gtcgaccgcg tgccgctccc
     4801 ggcgttcccg atatccacga ccgccaccac cactccgtcg cggaccagga cattgtcggg
     4861 cttgagatcg gcatggacca tgtcgggtgc gccttgtggt ggcgagacgt tggcacacat
     4921 gagcctgatg cgctcgacca gggccgac
//
LOCUS       DASTMD010000852         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_257793, whole genome shotgun sequence.
ACCESSION   DASTMD010000852 DASTMD010000000
VERSION     DASTMD010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257793"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFQ54_12655"
     CDS             complement(<1..345)
                     /locus_tag="VFQ54_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0165888.1"
                     /translation="MTTADVLVIGGGTMGTAAGWALARAGHSVIVLEQFGHVHTFGSH
                     GGKTRIFRHAYAEGEQYVPWTLDADRLWSELQQRSGKEFMYRTGGLDISAEDGGWAHQ
                     ARSSAERYGIPHE"
     gene            complement(514..1302)
                     /locus_tag="VFQ54_12660"
     CDS             complement(514..1302)
                     /locus_tag="VFQ54_12660"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEU0165889.1"
                     /translation="MANDIRVTVWNEFRHEKLDEHIGSIYPIGIHGAIAEGLNAIDGL
                     TATTATLDEPEHGLTQEVVDNTDVFVWWGHMAHGEVSDEIVTRVQERVLAGAGLIVLH
                     SGHYSKIFRRLMGTTCSLKWREADEKERLWVVSPGHPIVKGIGEYIEIAQEEMYGEHF
                     DIPQPDELIFVSWFQGGEIFRSGAAYSRGAGKIFYFRPGHETYPTYYNDQIRTVIGNA
                     TKWAAPVERAEERFFGNVDPIEDLPWYDRKHAVTEGFGSGKVGA"
     gene            complement(1395..>2319)
                     /locus_tag="VFQ54_12665"
     CDS             complement(1395..>2319)
                     /locus_tag="VFQ54_12665"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0165890.1"
                     /translation="NDEVDAVSIAVPNFMHAPVAIAALDAGKHVLIEKPLARNEEEGR
                     QMVEAAERNGKILAISYQRRTRHDVQIVREQVQSGAFGRVYYSKAFWMRRSGIPGWGS
                     WFTSKEAAGGGPLIDLGVHVLDLALYVLGNPRVTSVSASTYAEIGTQGRGNWVGASNR
                     FVPEGHKGYEVEDLASAFIRFEDGGTLLLEASWASYGEMEDDFGIQVYGSQGGARIHS
                     VKYSTSDTLRIFSGIADTTQVSAPVVVAREGHAGITRGFVDGILNGTPVSPDGYEGLD
                     RVRLIDAIYRSAELVREVLLEDVPVSTAVAE"
BASE COUNT          410 a          778 c          714 g          417 t
ORIGIN      
        1 ctcgtgagga atcccgtatc gctccgcgct cgatcgcgct tggtgcgccc agccgccgtc
       61 ctccgcggag atgtcgagac cgcccgtccg gtacatgaac tccttgccgc tacgctgctg
      121 gagctcggac cagagccggt ccgcatcgag cgtccaggga acatactgct cgccctcggc
      181 ataggcgtgg cggaagatac gcgtcttccc gccgtggctg ccgaaggtgt ggacgtgacc
      241 gaactgctcg agcacgatca cagaatgacc ggccctcgcc agtgcccaac ccgcggcggt
      301 tcccatggtg ccaccgccaa taaccagaac atccgccgtt gtcacgccca tcatcatctc
      361 cgccatacag tgcgaccgtt acatcggggc attatcgcgg aatcgtgtcg caacgggatg
      421 atacgttgcc gcggaccatc acaacacgag aacggaccgg gagacagctc ccggtccgtt
      481 ctcgcggatg tccgaatacg actcaacccg aggtcaggcg ccgaccttgc ccgaaccgaa
      541 accttcggtc accgcgtgct tgcggtcgta ccacggcaga tcctcgatcg gatcgacatt
      601 gccgaagaat cgctcctcgg cgcgctcgac cggagccgcc cacttcgtcg cgttgccgat
      661 gacggtgcgg atctggtcgt tgtagtaggt cgggtaggtc tcgtgacccg ggcggaagta
      721 gaagatcttg cctgcgccgc ggctgtaggc cgcgcccgaa cggaagatct caccgccctg
      781 gaaccaactg acgaagatca gttcgtccgg ctgcgggatg tcgaagtgct cgccgtacat
      841 ctcttcctgg gcgatctcga tgtactcgcc gatgcccttc acgatcggat gccccgggga
      901 cacgacccag aggcgctcct tctcatccgc ttcacgccac ttcaggctgc atgtcgtacc
      961 catcaagcgg cggaagatct tcgagtagtg accggagtgg aggacgatca ggccagcgcc
     1021 cgcgaggacg cgctcctgga cgcgggtcac gatctcgtcg ctgacctcac cgtgcgccat
     1081 gtggccccac cagacgaaga cgtcggtatt gtcgacgact tcctgggtga ggccgtgctc
     1141 cggctcatca agggtggcgg tcgtcgccgt gaggccatcg atcgcgttca gaccctcggc
     1201 gatcgcgccg tggataccga ttggatagat cgagccgatg tgctcatcca gcttctcgtg
     1261 acggaattcg ttccagacgg tgacgcgaat atcattcgcc attgggatac tcctgacggt
     1321 tgcgatcggt cgactgttgc gcggattact ctatgggccg cgtcgcgact tggcaaccgc
     1381 ccacgcgaaa tccgctactc cgcgacggcc gtcgagacgg gaacgtcctc gagcaggacc
     1441 tcgcggacga gctcggccga gcgatagatg gcgtcgatca ggcggacgcg atcgagcccc
     1501 tcgtatccgt ccggcgaaac cggcgtcccg ttgagaatgc catcgacgaa gccacgggtg
     1561 atcccggcgt gtccctcccg cgcgacgacg accggtgcgc tgacctgcgt ggtgtcggca
     1621 atgccggaga agatgcgaag cgtatccgag gtagagtact tcaccgagtg gatgcgcgcg
     1681 ccgccctggc tgccgtaaac ctggatcccg aaatcatctt ccatctcgcc gtaggatgcc
     1741 caggatgcct cgaggaggag cgtgccgcca tcctcgaacc ggatgaaggc agaggcgagg
     1801 tcctcgacct cgtacccctt gtgcccctcc ggcacgaatc tgttgctcgc cccgacccag
     1861 ttcccgcggc cctgcgtgcc gatctccgcg tacgtcgacg ccgaaaccga cgtcaccctc
     1921 ggattgccga gaacgtagag cgcgagatcg agtacgtgga cgccgagatc gatcaacggc
     1981 ccgccgccgg cggcttcctt cgacgtgaac cagcttcccc agccggggat gccgctccgg
     2041 cgcatccaga aggccttgct gtagtagaca cggccaaacg cacccgactg gacctgctcg
     2101 cgcacgatct gcacgtcgtg gcgcgtgcga cgctggtagg agatcgcgag gatcttcccg
     2161 ttgcgctcgg cggcctcgac catctgccgt ccctcttcct cgttccgggc gaggggcttc
     2221 tcgatcagga catgcttgcc ggcatccagc gcggcgatcg caaccggggc atgcatgaag
     2281 ttcgggacgg cgatgctcac ggcgtcgact tcgtcgttc
//
LOCUS       DASTMD010000853         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2402545, whole genome shotgun sequence.
ACCESSION   DASTMD010000853 DASTMD010000000
VERSION     DASTMD010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402545"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFQ54_12670"
     CDS             complement(<1..306)
                     /locus_tag="VFQ54_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165891.1"
                     /translation="MSDDFSNQLDDGLDPRDTIGRDRLSLDPVRLSLIDAGFYAYGTI
                     DDQNRWTIAVDDEAGRVDVRVGDDGLDVILWASSPGLYADEESEWRRKSRARLARITL
                     "
     gene            730..>2381
                     /gene="glgP"
                     /locus_tag="VFQ54_12675"
     CDS             730..>2381
                     /gene="glgP"
                     /locus_tag="VFQ54_12675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEU0165892.1"
                     /translation="MISRQLTTPAALPERIHRLHDLAFNLWWTWSKPAREIFAQLHPV
                     LWDLVEQNPVLFLNRIDAKRLNDAATNPAYLADYDAVVAAFDAMLNTAQTDTWLGQNR
                     PELVGKTLAYFSAEFGLHRALPIYSGGLGVLAGDHTKEASDMGIPLVGISLLYRQGYL
                     RQRIDSGGWQHDVPGDLDPGAEPTMQVLDDNGIPVQIEVELGAEKIRLEVWHVQVGRV
                     PLYLLDSDVDGNPDWTRQLSSRLYGGDTEHRLRQEIILGIGGVRLLRKLGIDPVYWHA
                     NEGHAALHLLERVREHVKAGLSFDVAREHVRATTVFTTHTPVPAGHDIYSADTMRRYF
                     SHYWPELGLDEASFLELGRHGEEPGFNFTALSLRLAANVNGVSVKHGEVSREMWHDLW
                     PDRTVDETPIISITNGVHLRTWISPEMRAVFERYLPRKWRDRQESEYVWELILNIPDD
                     EFWAAHQVSKQHLIDEMDSRARRRYVEGQFDAWQVLSSGPFQSANVLTIGFARRFATY
                     KRATLILQDIDRLASILNNPDYPVQLVFAGKAHPADEGGKRL"
BASE COUNT          436 a          734 c          748 g          463 t
ORIGIN      
        1 gagcgtgatc cgggcgagac gcgcgcggga cttgcggcgc cattcgctct cctcgtccgc
       61 atagagcccc ggagaggaag cccagagaat gacatcgaga ccgtcatcgc cgacgcgaac
      121 gtcgacccgt cccgcctcgt catcgaccgc gatggtccac cggttctggt cgtcgatcgt
      181 gccataggcg tagaagccgg catcgatcag tgacaggcga accggatcga gggagagtct
      241 gtcccggccg atggtgtcgc gtggatcgag gccatcgtcg agctggttgg agaaatcgtc
      301 cgacactgcg gaaagctcct gtcgatcaac tgctgtgcgc ggcgctggag gccggcgact
      361 ttactcgtat tgccccatga tgccatgaag atcgggcggt gcggaccgtc gaactggcga
      421 atccggggtg cgatccgggg caattcgaac gcgactcgtt gtcgcgatac aggatcgcct
      481 ctccattagg caacgattgt actggactcc tgttcagtgt cttccttgat acatggcttt
      541 catatggaaa tgacgatttc tgcacggtac tcttcgatcg ttgcattatt gcagtcgtcg
      601 ggcgaagctg gagtcgcctg aatccaacac agttcatcct gaacctgcgc gctcccacga
      661 gagcgccgcg acgttgcggc cgggcagcga atgaccggcc ccacggctac cttgtggagt
      721 tatgcattca tgatctcgcg acaattgacg acgcccgcgg cgctaccgga gcggattcac
      781 cgcctgcacg acctcgcgtt caatctctgg tggacctgga gcaagccggc gcgcgagatc
      841 ttcgcgcagc ttcacccggt gctctgggat cttgtcgaac agaatcccgt gctcttcctc
      901 aaccggatcg atgccaagcg gctgaatgac gcggcgacga atcccgccta tctcgccgac
      961 tacgacgcgg ttgtcgccgc gttcgatgcg atgctcaata ccgcgcagac cgacacctgg
     1021 ctggggcaga atcggccgga actcgtcggg aagaccctcg cgtacttctc agccgaattc
     1081 ggccttcatc gggcgcttcc gatctactcg ggcggtcttg gggtgctcgc cggcgaccac
     1141 accaaggaag cgagtgatat gggaatcccg ctcgtcggga tctcgcttct ctatcgccag
     1201 ggatacctcc gtcaacggat cgattccggt ggctggcaac atgatgtgcc gggtgatctc
     1261 gaccccggag ccgagccaac gatgcaggtg ctggatgaca acggcatccc cgtccagatc
     1321 gaagtggagc ttggcgcgga gaagattcgg ctcgaggtct ggcatgtcca ggtgggccgg
     1381 gttccgctct atctcctcga ttccgacgtc gatggcaacc cggactggac gcgacagcta
     1441 tcgtcgcgac tctacggtgg ggacacggag catcgtctgc gccaggagat catcctcggc
     1501 attggtggcg tgcggctcct tcgcaagctg ggcatcgatc cggtgtactg gcatgcaaac
     1561 gaggggcacg cggcgctcca tcttctcgag cgtgtgcgcg aacacgtcaa ggcgggcctc
     1621 tcattcgacg tggcgaggga gcacgtccgg gcgacgacgg tattcacgac ccacacgccg
     1681 gtgccggcgg gacacgatat ctactcggcc gacaccatgc gccgctattt ctcccattac
     1741 tggccggagc tcggactcga cgaggcttcg ttcctggagc ttgggcgaca tggcgaggag
     1801 ccgggattca acttcacggc gctttccctg cgtcttgccg caaatgtcaa cggcgtcagc
     1861 gtcaagcacg gtgaggtctc ccgcgagatg tggcacgatc tctggcccga ccggacggtc
     1921 gacgagacgc cgatcatctc gatcacgaat ggcgttcacc tccgcacctg gatctcgccg
     1981 gaaatgcgcg ccgttttcga gcgatacctg ccgcggaagt ggcgcgatcg tcaggagagc
     2041 gagtacgtct gggagctgat cctgaacatc ccggacgacg aattctgggc ggcgcatcag
     2101 gtatcgaagc agcacctgat cgacgagatg gattcgcgtg cgcgccgccg gtacgtcgag
     2161 gggcagttcg atgcctggca ggtgctttcg tcggggccgt tccagagtgc gaatgtcctc
     2221 acgatcggct ttgcccgtcg cttcgcgacc tacaagcggg cgacgttgat cctccaggac
     2281 atcgatcgcc tcgcaagcat cctcaacaat ccggattatc cggttcaact cgtcttcgcc
     2341 gggaaggcgc atccggcgga cgagggtggc aagcgcctga t
//
LOCUS       DASTMD010000854         9513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2402556, whole genome shotgun sequence.
ACCESSION   DASTMD010000854 DASTMD010000000
VERSION     DASTMD010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402556"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(389..802)
                     /locus_tag="VFQ54_12680"
     CDS             complement(389..802)
                     /locus_tag="VFQ54_12680"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0165893.1"
                     /translation="MRTMIFPNLPVNDLPKATAFYEALGYKRNTQFSNDDASAIMISE
                     EIVVMLLTRPFFGTFTSKTIIDATSSVEMSLALSADSREDVDALVEKALAAGARKPSE
                     AQDLGFMYSRAFEDLDGHHWEVMWMNPATVEPYEV"
     gene            1088..1288
                     /locus_tag="VFQ54_12685"
     CDS             1088..1288
                     /locus_tag="VFQ54_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165894.1"
                     /translation="MKWFKSLTTVLYVEGVKRGDRPPYQHRFWQRNYYDRVIRDDKEL
                     EFVHDYIENNPATWFEDSRFTP"
     gene            complement(1439..2365)
                     /locus_tag="VFQ54_12690"
     CDS             complement(1439..2365)
                     /locus_tag="VFQ54_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165895.1"
                     /translation="MTDTTSELKSRPTPGPDAVALAAADRQNEGARKNRVWRRFARYR
                     PGVVGLGFIVLLAFCAIAAPLISPYDPDAVNPKLRGHAPSLAHWLGNDDIGRDILSRI
                     IYGTRVALIVGLGATLIAVVIGVSVGAAAGYFGGKADFWLSRFIDTLMAFPLLPLLLT
                     LSTVFGPSLRNVVIVIGCVIWASYARVVRAEVLSLREQDYILAARSIGATSRSIITRH
                     LIPNAIGPVIIIASLSVGGIIILESALSFLGLGVQRPTASWGTMLSDGREFIRNYPHI
                     EIAPGLAIAFTVLAFNLVGDGLRDALDPRERR"
     gene            complement(2415..3341)
                     /locus_tag="VFQ54_12695"
     CDS             complement(2415..3341)
                     /locus_tag="VFQ54_12695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0165896.1"
                     /translation="MASYIAKRLIQGVIVLFLVSLLTFFVMYFMPGDPARQMVGQAYV
                     TDAQLDLIRDKWGLNDPWYERYGTWIGNVVTGNFGTSMMRPGMGITTMIGDAAGMTLR
                     LTTLSFLISLCIAIPVGIIAAVKRYSWFDYGSMLGSTLGVAIPNYWIGLMGIVIFSIK
                     LGWLPPYGSDGWKSYVLPVIVLSIEEIAALARITRGATIGVLSMDYVTTARAKGLPFH
                     QIVRAHVVRNTLLPVITIIGYRIAFILSGTIVVETVFAWPGLGRLFYQAITSQDLQVV
                     QAIVLLLTALVVVANIITDLTYAFIDPRVRIS"
     gene            complement(3544..5283)
                     /locus_tag="VFQ54_12700"
     CDS             complement(3544..5283)
                     /locus_tag="VFQ54_12700"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0165897.1"
                     /translation="MNGSTTHDEGATNANAERRSHLQLLWEMTGSRRRFLAAAAAAGL
                     IPVLGSARAVAAQDAQPARGGTFVTISQDTIESLSPEDSAETAQWVVIAQMFDGLYNV
                     NENYELEPVLADSYEASPDGKTYTFKVKSGVKFHNGDEFTSADVKYTYDWILDPKNGS
                     TRSGEFELVDSVEAPDATTVVVNLKAADVTFMVNVAPVFIFPSKYHAQTGEDAFKGKP
                     VGTGPFKLKEWIPAQNTTLDAYDDYFRGRANFDEVRVDVVPEAAGRAAALESGQADNS
                     IWALNAEDNDSIKSSGDFKIYETLQNAVNHFVLNNTPVGGFPVDASATPVADDKKPQE
                     QPFLSEVAVRQALLHALDRQSLADDIFKGQAQVATSNLSPAVAAYYNPDVPKYDFDPD
                     AAKKLLDDAGWTVGGDGTRAKNGNKASFTLIVFQGDTQRRPEAELAQQWWKDIGVECK
                     IQEGITTDTLDGMVEGRLQAALFNWVYGGGAGEPDARDTLSSHGADNFCNFRNAEVDQ
                     LLDQGIVTQDHGKRVEIYNRIQEIVAEQVPFLYLLVLKSMAFFANRIKGLPDQVLSSD
                     NLYPKLYKFWISE"
     gene            complement(5545..7197)
                     /locus_tag="VFQ54_12705"
     CDS             complement(5545..7197)
                     /locus_tag="VFQ54_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0165898.1"
                     /translation="MSETERRSRLQQLMSVTGSRRQFLTAAAAAGLIPVLTTETLTAR
                     AAAAQATPAPTPVKGGEFITLGHQTVDTLSPDNEGATVVWACVVQMFYALYIVNENYE
                     IVPILAESHEVSTDGKTYTFKLKSGVKFHNGDDFSSADVKYTYDWIHDPKNASLRAGA
                     FELVASVEAPDPTTVVVNLTQADVTFMVNVAPTLIYPATYHAKIGEEAFTAKPVGTGP
                     FKLKEWIPAQSTTLEAYDDYFRGRPNFDTFRLDVVPEAAGRTAALESGQADSSVWSLS
                     AEDNNSLEDSGNYTVYKNPSLAVNHFPLNNAHPVLKDKAVRQALIYATDRQALADNVY
                     LGQAAIATSNLSPSNPKYYNADVPKYPLDVDKAKSLLDAAGWVPGDGGIRSKDGVKAS
                     FTLQIIQGDTQRRPEAELAQQWWKEIGIDCQLAETNNALAGLVAGQYDAGLFNWTYGG
                     VDPDARDTLTTKGANNFNHFSNAEVDDLLTKGVSELDEAKRITMYKRVQEIVAEEVPF
                     IFLLYVQWITFWNTDIKGLPPQEKVQDSSGIYNEIYKLWKES"
     gene            complement(7673..8737)
                     /locus_tag="VFQ54_12710"
     CDS             complement(7673..8737)
                     /locus_tag="VFQ54_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165899.1"
                     /translation="MTMRLGKRTARIAFAFLLVLLAGSSTLGRTTAAAVQDAGSERSD
                     PIAVGDAGTIGDYDITVLEVTPNADDVVAAENQFNEPPAEDEQFYIARISVTYTGSDK
                     GTPASDLNFQAVGTSNSSYTTFDNSCGVVPDDPFSTSELFEGGVVEFNICWAVASIDV
                     DSLVMYVEPLFSLNDEPVWFSLGSKDEGTPPASGVKKSDAKVVKDSSREKPIPFGSAG
                     VSGDYQISVIDVTPDGNDVIAEENQFNDPPAEGNQFFLVKVSATYIGSDTGNPAVDLN
                     FQAVGDKSVGYTTFDNSCGVTPDEQYSVTELFKGGSAEFNVCWQIDSADASSLVMYVE
                     PLFSLRSDSVWFSLDPAKAS"
BASE COUNT         1795 a         2955 c         2940 g         1823 t
ORIGIN      
        1 tttcgccgcc ttggttcccc attttctctc tgaagttgat gcgcctgcca ctgcatttct
       61 aatgaatgct tgccgatcct gctttccatc tccacctctc tgtatatggg taatcaggcg
      121 acgacgcacc ctggagctcc ctgaccggtg tctcgtcccg gggtgcggaa cgactaccgg
      181 taacgtcccg ggcgagcgtg ggccacagca agggcgaccc ggaagatcgt ccattccgcg
      241 gtcgcgacct ccgggttcgg acgacctcaa actgaccccg atcgcgaggg aatgcactca
      301 tgggacatgg aaatccaacg caaggcagga ggcggatcgt gatgacccgc ctcctctttc
      361 cgttttgtcg tgatggaccc gtctggaact agacctcgta cggctcgacg gtcgccgggt
      421 tcatccacat cacttcccag tggtggccat ccaggtcctc gaatgcgcgg ctgtacatga
      481 acccaaggtc ctgcgcctca cttggcttcc tggcgccggc cgcaagcgcc ttctcgacga
      541 gcgcgtcgac gtcctcacgc gagtccgcgc tcagggcgag cgacatctcg acgctgctgg
      601 tcgcgtcaat aatcgtcttc gacgtgaagg tgccgaagaa cggacgggtc agcagcatga
      661 ccacgatctc ctcgctgatc atgatcgcgc tcgcatcatc gttcgagaat tgcgtgtttc
      721 gcttgtatcc gagcgcctcg tagaacgccg tcgccttcgg aaggtcgttc accggcagat
      781 tggggaagat catggtccgc atggtcgatt ctctccagtc gatcgagcag ttccttcgct
      841 cgtgcgcccc gcgcacgtgg gaacatcgtt gcacccacgt ccatcctcgc acggtcgatc
      901 gtgcgctgct tcttccgcct tgcgagacct ccgggcagcc tcgatcagca caacaccggc
      961 cccgccggac cggcgggatc gtccacgcac ggcacagaat ccgggcgatc ctggtggtcg
     1021 cccatacgca tcacgttttc tggttcgaag acgcggccgg cggtatctat ccctccttgg
     1081 ggacatcatg aaatggttca aatcgctaac cactgttctc tatgttgaag gcgtcaagcg
     1141 cggtgaccgg ccgccctacc agcaccgatt ctggcaacga aattactatg atcgcgtgat
     1201 tcgggacgac aaggaactgg agttcgtcca cgactatatc gagaacaatc ctgcaacgtg
     1261 gttcgaggac tcacgcttca cgccgtaggc cggaaccgcg aatgcgtgga atcgccaatg
     1321 gcgagtccag ggcgatcacc aggatcgccc cctacgaggc atcgcaccgc aggcaggcac
     1381 cgaaaacgag ggttccggat gaccatgtat tcgggaccaa tcgtcccccc aatacggcct
     1441 accggcgttc gcgcggatcc agggcgtcgc gaagaccatc gccgacgagg ttgaaggcga
     1501 ggacggtgaa cgcgatcgcc aggcccggcg cgatctcgat gtgcggatag ttgcggatga
     1561 actcgcgacc atccgaaagc atcgtgcccc aggaggccgt cgggcgctgg acgccgagac
     1621 cgaggaacga gagtgccgac tcaaggatga tgatgccgcc gaccgagaga ctcgcaatga
     1681 tgatcaccgg tccgatcgcg ttcggaatca ggtgcctcgt gatgatcgag cgactcgtcg
     1741 ccccgatcga gcgcgccgcc aggatgtagt cctgctcgcg caggctcaac acctccgccc
     1801 gtaccacccg cgcatacgac gcccagatca cgcagccgat gacgatcacc acgttgcgca
     1861 ggctcggccc gaacaccgtc gagagcgtca ggagcaacgg cagcaacggg aaggccatca
     1921 gcgtgtcgat gaagcgggag agccagaaat cagccttgcc gccgaaatac ccggcggcgg
     1981 cgccgacgct gacgccgatc acgaccgcga tcagcgtcgc gccgagaccg acgatcaagg
     2041 cgacccgcgt cccgtagatg atgcgactca ggatgtcgcg cccgatatcg tcgttgccga
     2101 gccagtgggc cagcgacggc gcgtgaccgc ggagcttggg attcacggcg tccgggtcgt
     2161 agggcgagat caggggcgcc gcgatcgcgc agaaggcgag aaggacgatg aagccaagcc
     2221 ccacgacgcc cggtcgatag cgggcgaaac gccgccagac gcgattcttg cgggcaccct
     2281 cgttctggcg gtcggcggcc gcgagtgcga ccgcgtcggg gccgggtgtc ggtcgagact
     2341 tgagttctga cgtcgtgtcg gtcatggcgt gagattccct cgagtcaaga ggcggaatga
     2401 cggctgatcc gaaattacga aatccggacc cgggggtcga tgaaggcata ggtgagatcg
     2461 gtaatgatgt tcgccacgac gacgagcgcc gtcagcagga gcacgatcgc ctgcacgacc
     2521 tggagatcct gcgaggtgat cgcctggtag aaaagcctcc ccagacccgg ccacgcgaag
     2581 accgtctcga cgacgatcgt gccgctgagg atgaaggcga tccggtagcc gatgatcgtg
     2641 atcaccggga gcaaggtgtt gcggacgacg tgggcccgga cgatctggtg gaaggggagc
     2701 cccttcgcgc gcgcagtcgt gacataatcc atcgacagca cgccgatcgt cgcaccccgg
     2761 gtgatccggg cgagggcggc gatctcctcg atcgagagca cgatcacggg gaggacgtag
     2821 gatttccacc catccgaacc gtatggtggc aaccacccta gcttgatcga gaagatgaca
     2881 atccccatca acccgatcca gtaattgggt atcgccacgc ctagcgtgga cccgagcata
     2941 ctcccgtaat cgaaccagga gtaacgtttt accgcggcga tgatcccgac cgggatcgcg
     3001 atacagagcg agatcaggaa cgagagcgtc gtcagccgca gggtcatccc cgccgcatcg
     3061 ccgatcatcg tcgtgatccc catgccggga cgcatcatcg acgtgccgaa gttcccggtc
     3121 acgacgttgc cgatccacgt gccgtaacgc tcgtaccagg gatcgttcag cccccacttg
     3181 tcgcggatca ggtcgagctg ggcgtctgtc acgtaggcct ggccgaccat ctgccgggcg
     3241 ggatctcccg gcatgaaata catcacgaag aaggtcagca ggctgacgag aaagaggacg
     3301 atgacaccct ggatcagtcg tttggcgatg taggaagcca tggctacgta tccctgcgtt
     3361 cgataaagcg aatcacgtca cgcgaaaacc agcgaacgtt gccgggattt ccgtcggacg
     3421 tcatcgatcg acggagagat gaacgttgca cgaatcacgc gagaccgggt caccagcggt
     3481 tgtgtgcccg ggcgggactc catcgtcccg cccggggatc tgtcccggat gcccgccctc
     3541 gtcctactcg gaaatccaga acttgtagag cttcggatag aggttgtcgg acgagagcac
     3601 ctggtccggc aatcccttga tccggttcgc gaagaacgcc atgctcttca ggacgaggag
     3661 atagaggaag ggcacctgct ccgccacgat ctcctggatc cggttgtaga tctcgacccg
     3721 cttcccgtgg tcctgggtca cgatcccctg gtcgagcaac tggtcgacct ccgcattccg
     3781 gaagttgcag aagttgtcgg cgccgtggga actgagcgtg tcgcgcgcat ccggttcgcc
     3841 ggcccctccc ccgtagaccc agttgaagag cgcggcctgg agccgccctt cgaccattcc
     3901 gtccagcgtg tcggtcgtga tcccttcctg gatcttgcac tcgactccga tgtccttcca
     3961 ccactgctgc gcgagttcgg cttccggtcg ccgttgcgta tcgccctgga agacgatgag
     4021 cgtgaacgac gccttgttcc cgttcttcgc gcgtgtgccg tccccaccca cggtccaacc
     4081 cgcgtcatcg agcagcttct tcgccgcatc cggatcgaag tcgtacttcg ggacatccgg
     4141 gttgtagtag gccgcgacag cgggcgagag attcgaggtg gcgacctgcg cctgaccctt
     4201 gaagatgtcg tccgccagcg actgccggtc gagtgcgtgg aggagggcct gccggaccgc
     4261 gacttcggac aggaacggct gctcctgcgg cttcttgtca tccgcgaccg gcgtcgcgct
     4321 ggcatcgacc gggaatccgc cgaccggggt gttgttcagg acgaagtggt tgaccgcgtt
     4381 ctgcaaggtc tcgtagatct tgaagtcgcc gctggacttg atgctgtcgt tgtcctcggc
     4441 attcagcgcc cagatcgagt tgtccgcctg cccgctctcg agcgccgccg cccgaccggc
     4501 ggcctctgga acgacatcga cccgcacctc gtcgaaattc gcgcggcccc ggaagtaatc
     4561 gtcataggcg tcgagcgtcg tgttctgcgc cgggatccat tccttcagct tgaacggacc
     4621 ggtgccgacc ggcttcccct tgaacgcatc ctcgccggtc tgggcgtggt acttcgacgg
     4681 gaagatgaag accggcgcga cattgaccat gaaggtcacg tcagctgcct tcagattgac
     4741 gacgaccgtc gtcgcatccg gggcctcgac ggaatcgacg agctcgaatt cgccggatcg
     4801 ggtcgaaccg ttcttgggat cgagaatcca gtcgtacgtg tacttcacat cggccgacgt
     4861 gaattcatcg ccattgtgga acttgacgcc cgatttcacc ttgaaggtgt aggtcttgcc
     4921 atcgggcgag gcctcgtacg aatcggcgag caccggttcc agctcgtagt tctcgttgac
     4981 gttgtagagt ccgtcgaaca tctgggcgat caccacccac tgtgcggtct cggcgctatc
     5041 ctccgggctc aggctctcga tcgtgtcctg cgagatcgtg acgaaggtgc cgccccgcgc
     5101 tggctgcgcg tcctgcgccg ccaccgcccg ggccgaaccc aggaccggga tcagacccgc
     5161 cgcggccgcg gccgcgagga atcgccgtcg cgatcccgtc atttcccaaa gcagctgcag
     5221 atgtgaacgc cgctctgcgt tcgcgttcgt cgcaccctca tcgtgcgttg tggacccatt
     5281 catcacgaga actcctccat ggcgccagat gtcgcttcga ccccgctggc tcgttcaggc
     5341 aacgggatcg ctgcgcacat ccacccagat gtgcgcgacg ttgcctctca agacactcga
     5401 ctcggcgaca ttcttgcaca atcgcgcgat tgtgaacaac ggccgaatac ggtcgcgcgg
     5461 tggttccatg gcgtcatgtt ggtcggaacg cacaacgcgt caccgaaagg ggtgacgcgt
     5521 tggcgatcga tcgtggcggc gaggctacga ctctttccag agcttgtaga tctcgttgta
     5581 gatcccggac gaatcctgga ccttctcctg gggcgggagg cccttgatgt cggtgttcca
     5641 gaaggtgatc cactgcacgt agagcaggaa gatgaacggc acctcttcgg cgacgatctc
     5701 ctgcacgcgc ttgtacatcg tgatccgctt cgcctcgtcg agttcgctga cgcccttggt
     5761 caggaggtca tcgacttccg cattcgagaa gtgattgaag ttgttcgcac ccttggtcgt
     5821 cagcgtgtcg cgggcatccg gatcgacgcc accgtaggtc cagttgaaga gaccggcgtc
     5881 gtactgaccg gcgacgagac cggcgagcgc attgttggtc tcggcaagct ggcaatcgat
     5941 gccgatctcc ttccaccact gctgcgcgag ttcggcctcc gggcggcgct gggtatcgcc
     6001 ctggatgatc tgcaaggtga aggacgcctt cacgccatcc ttcgaacgaa tccctccatc
     6061 gccgggaacc cagccggcgg cgtccagcaa cgacttcgcc ttgtcgacat cgagcggata
     6121 cttcggcacg tcggcgttgt agtacttcgg gttggacggc gagaggttgg acgtcgcgat
     6181 cgcggcctga ccgagataga cgttgtcggc gagggcctgg cggtcggttg catagatcaa
     6241 cgcctggcga accgccttgt ccttgagcac cggatgcgcg ttgttgagcg ggaagtggtt
     6301 caccgcgagg ctcgggttct tgtagacggt gtagttcccc gaatcctcca gcgaattgtt
     6361 gtcctcggcg ctgagcgacc agacggagct gtcggcctgg ccactctcga gagcggcggt
     6421 gcgaccggcg gcttccggca cgacatccag gcggaaggtg tcgaagttcg gacgaccgcg
     6481 gaagtagtcg tcgtaggcct cgagcgtggt gctctgggcc gggatccact ccttcagctt
     6541 gaacggaccg gtcccgaccg gcttggccgt gaatgcctct tccccgatct tggcgtgata
     6601 ggtcgccgga tagatgaggg tcggcgccac gttgaccatg aaggtcacgt cggcctgggt
     6661 gaggttgacg acgacggtcg tcggatcggg agcctcgacg gacgcgacga gctcgaaggc
     6721 gccggcgcgg agcgaggcgt tcttcggatc gtgaatccag tcgtaggtgt acttcacgtc
     6781 ggcggaggag aaatcgtcgc cgttgtggaa cttgacgccc gacttcagct tgaacgtgta
     6841 agtcttgcca tccgtcgaga cctcgtggga ctcggcgagg atcggcacga tctcgtagtt
     6901 ctcgttgacg atgtagagcg cgtagaacat ctggaccacg caggcccaga cgacggtagc
     6961 gccctcattg tccggcgaga gcgtatcgac ggtctggtga ccgagggtga tgaactcgcc
     7021 acccttgacc ggcgtcgggg ccggggtggc ctgcgcagcg gccgcgcgag cggtgagggt
     7081 ctccgtcgtg aggacgggga tgagaccggc tgcggcggcg gcagtgagga actgccggcg
     7141 ggagccggtc acgctcatca actgctgaag gcgactgcgt cgctcggttt cgctcaacgg
     7201 cgagctatcg ttcgaacccg gtttttgctg ctgtgacact gcgtctctcc atcgtcgtgc
     7261 gacattacgg cggttcggtt cgagaccctc atctcaccgc ggggtcgcgt ggtgagaccg
     7321 tgagcgtcat gcacgaattg cccccaaccg gggaaaacgt ggcatgagat cgtttatcag
     7381 tcttgcacag cagccgggca cgggcaacga actcaaaagg atcactttcg attgttgcgg
     7441 gcggctcttg gtccgcgggc acaaaccatc ggtcatcgtg cttgtcgaac cgtcggtttc
     7501 gcacacaaaa cgtccccgtc gccttgaagc gacggggacg tttgctgatt gaacgggatt
     7561 tccgttattc gaccgtcaac gtcccaacca tgccggccac tttatgccca ggaaccgagc
     7621 aatggaactg atacgttctc gcccgaaggt tgatggtgat cgaagcctaa tgtcatgacg
     7681 cctttgcggg atcgagcgag aaccagaccg aatcgctcct gagcgaaaac agcggttcaa
     7741 cgtacatgac gagtgatgac gcgtccgcgc tgtcgatctg ccagcacaca ttgaactcgg
     7801 cagaaccacc cttgaagagt tcggtcacag aatactgctc gtcaggcgtc acgccgcaac
     7861 tgttgtcgaa cgtcgtatat ccgacactct tgtcaccgac agcctggaag ttgagatcga
     7921 ccgcgggatt cccggtatcc gatccgatat aggtggccga gactttcacg aggaagaact
     7981 ggttcccttc ggctggagga tcattgaact gattctcctc ggcaatcacg tcattgccat
     8041 ccggtgtgac gtctatgacg ctgatttgat agtcgccact cacacccgcg cttccgaatg
     8101 gaatcggctt ctcgcgcgaa ctgtccttga ccaccttggc gtcggatttc ttgacgccgc
     8161 tggcgggcgg tgtcccctcg tccttgcttc cgagcgaaaa ccagaccggc tcgtcgttca
     8221 ggctgaagag gggctcgacg tacatcacaa gtgaatcgac atcgatggac gcaaccgccc
     8281 agcagatatt gaactcgacc acaccgcctt cgaagagttc ggatgtgctg aacggatcgt
     8341 ctggcaccac gccgcaactg ttgtcgaagg ttgtatatga agagttgctc gtaccaacag
     8401 cctggaagtt caggtcagac gcgggtgttc ccttgtcact gccggtatag gtgactgaga
     8461 tccgggcaat atagaactgt tcgtcttctg ccggaggctc attgaactga ttctctgcgg
     8521 caacgacgtc atccgcattc ggcgtcacct cgagaaccgt aatatcgtag tcaccgatcg
     8581 ttccggcatc cccgacggcg atcgggtcgc tacgctcgct accggcgtct tgcaccgccg
     8641 cggcggtcgt gcgcccgagg gtggacgaac ctgcaagcag cacgagcaag aaggcaaacg
     8701 caatccgggc agtccgcttt cccaaacgca tggtcatccc acctcgatat atatagagat
     8761 tcacgccgat cgcacaacgc gatccgcagc cccgaagaca tgattcgtgc ggcactgaaa
     8821 gtccgtgttc cctgctgaaa ttgtagacga tcgactcgat cctagaaaca tattgggcag
     8881 atattgacga gtctttcgca ttcgcacgtc gggcgacgcg catcgccctc ccggttgggt
     8941 gggtgcgccc gtccaaaccc accacagtac tccgtcgaga aacgggatgc tgagtagggg
     9001 ccgcgtccaa acgggccggg gtagcagcgc aggcgggaca ggccgtccgg actgcggtcc
     9061 ggcagcggcg atcccgccct acgaacgacc gaatccagcc gcgaccgcgc cgtgctcact
     9121 ggcgcctgtt cggttcccgt cctggcgacc catgtggtcg ccgtggatcg gcgctgccga
     9181 tccgtgtccg gggagcgtct tgcgccgcgt gttcccgttg gcgtccgcct gcggcggatg
     9241 tgcttcgcac ggggatttct cgacaggctc gaaatgacaa accttcatcc gacggccttg
     9301 gagtctacct ctcgtgacaa aagggaacac ccgtgcttga ggtctcgcag cgaacgatcg
     9361 ttcactgtgc cgcatcgaca cggaacggcg acgagccctg gtcgaaccaa cgcccggtac
     9421 ggcatggcgg ggtgccaggg cgccgatcct atcctgccac gagggcgtcg acgatcatcc
     9481 ggaagaggcg cagatcctcc cggaaatcct cgg
//
LOCUS       DASTMD010000855         1590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1152330, whole genome shotgun sequence.
ACCESSION   DASTMD010000855 DASTMD010000000
VERSION     DASTMD010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152330"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /gene="tatC"
                     /locus_tag="VFQ54_12715"
     CDS             complement(<1..769)
                     /gene="tatC"
                     /locus_tag="VFQ54_12715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU0165900.1"
                     /translation="MASVVRMIGGASARAVVKTARAVRDPLKPYRAWKDIYRTPADTP
                     EVFEEMTLQEHLVEVRDRMMKVVFAIIPLFIVGFYFAGNILDAIAKKANASQGLDVRS
                     PTDPLTLTFKIALYVALSLGMPIIVYQIVAFLAPGLTRREKRVLYTALPFVSILFIGG
                     AAYGYFVAAPRALWFLSNWNKGAFMWQPDGPEVISFFLTLMIGLGISFQLPVIMFILA
                     KIGIVTPKLMRKWRRYSILIIAIAAAVITPSTDPFNMM"
     gene            complement(782..1000)
                     /locus_tag="VFQ54_12720"
     CDS             complement(782..1000)
                     /locus_tag="VFQ54_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165901.1"
                     /translation="MSHLYQPHTKRQRKCRLITKLPITFGEPIKPTAGYLVDHRHPWS
                     TSTATRDQGAFPQIAHNAEQTVSSQSYN"
     gene            complement(1338..>1590)
                     /locus_tag="VFQ54_12725"
     CDS             complement(1338..>1590)
                     /locus_tag="VFQ54_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165902.1"
                     /translation="RIIVVALDTGIEYRSEESAPAVIMQTLSGGVLVTAISTPVELRP
                     GGMIHLAAGFSFSAIALDPTHLQITTFGGTPGPDSDDES"
BASE COUNT          323 a          426 c          540 g          301 t
ORIGIN      
        1 tcatcatgtt gaagggatcc gtgctcggtg tgatcaccgc ggctgcgatg gcaatgatga
       61 ggatcgagta gcgtcgccac ttgcgcatca gcttcggggt gacgatgccg atcttcgcga
      121 gaatgaacat gatgaccggt agctggaacg agatgccgag tccgatcatc agcgtaagga
      181 agaagctgat cacctcggga ccgtcgggct gccacatgaa ggcgcccttg ttccagttcg
      241 agaggaacca gagcgcgcgg ggagcggcta cgaagtagcc atacgccgcg cctccgatga
      301 agaggatgga gacgaatgga agggccgtgt agagaacgcg cttctcgcgt ctcgtgaggc
      361 cgggggcgag gaaagccacg atctgataga cgatgatcgg catgcccagg gacagggcaa
      421 cgtacagcgc gatcttgaag gtaagggtaa gcggatcggt cgggctgcgg acatcgaggc
      481 cctgggaggc gttggccttc ttcgcgatcg cgtcgaggat gttgcccgcg aagtagaagc
      541 cgacgatgaa gaggggaatg atcgcgaaga cgaccttcat catgcgatca cgaacttcga
      601 cgagatgctc ctggagcgtc atctcctcga agacctcggg ggtatcggcg ggagtccggt
      661 aaatgtcctt ccaggctcga tatggcttga gaggatcgcg aacggcgcgc gcggtcttca
      721 caacggcgcg agcggaggcg ccaccgatca tgcgtacaac ggatgccacg gtgggactct
      781 cctagttata ggactgtgaa gacacagtct gctcggcgtt gtgcgcgatc tgcggaaagg
      841 cgccctgatc ccgggtcgcg gtggacgtgc tccagggatg gcggtgatcc acgagatacc
      901 cggcggtcgg cttgattggc tcgccaaatg tgatcggaag tttcgtgatg agccgacact
      961 tgcgctgtcg cttcgtgtgc ggctgataga gatgactcat gtcctcgctc cctggtgatt
     1021 gccgtcgacc gtgtcgacca acatcaccgg cacagtagct gtgcgagaga cgatccatgg
     1081 catgcggcaa atctgggacg accagaatgg ttacacggtt cgtcatcata ccggagggat
     1141 tccggcggac ggcagcctgg cgggggacgc ccggctcgtt ctcatttgtc gtgtaccagg
     1201 gtcgcgtgct cgcgaaattg cccgagaaac tccaagaagg ttatcacacg gtcacgaatc
     1261 ggcgggaaca gagtctcctg ccgattgcta tacgttcccc cgacggtcgt ggatctgcga
     1321 gcttcctgag aaagttctca ggattcgtcg tcggagtcgg gacccggtgt tccgccgaac
     1381 gtggtgatct ggaggtgggt tggatcgagc gcaatcgcgc tgaacgagaa tccggccgcg
     1441 aggtgaatca tgccgccggg acgaagctcg accggcgtac tgatcgcggt gacgaggacg
     1501 cctccactga gggtctgcat gatgacggcc ggtgcggact cctcgctgcg gtactcgatg
     1561 cccgtgtcga gcgccacgac gatgatccgc
//
LOCUS       DASTMD010000856         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_79926, whole genome shotgun sequence.
ACCESSION   DASTMD010000856 DASTMD010000000
VERSION     DASTMD010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79926"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFQ54_12730"
     CDS             <1..544
                     /locus_tag="VFQ54_12730"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HEU0165903.1"
                     /translation="VGATYIIAEGPDGLFMIDQHAAHERVMYEKIQAQMVEQSVDRQP
                     MLDPLVIELPPDELVAFERSVAELNAIGFEIDRFGEQSVAIRAVPAIVKGVDISERLH
                     DILQELVAGGAGTSWLDSVAISAACHTAIRAGQALSLSEMRELVAQLERTSQPRACGH
                     GRPTMLHMSQHELERQFSRR"
     gene            complement(1000..1812)
                     /locus_tag="VFQ54_12735"
     CDS             complement(1000..1812)
                     /locus_tag="VFQ54_12735"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEU0165904.1"
                     /translation="MNKISSSELAPVSDQISGAARAVSTAVAIVLPAYNEQERIAATL
                     ETVWRFFAERRIAAEIIVADDGSTDNTLEIANAFAASHRNITVLALPHGGKAKAVLAG
                     LARSNSPIAGFTDVDLAAPLDTWDRCADAFRSGFDVAIASREGSDAARIGEPAYRHVM
                     GRVFNGMVRFLLLPGIHDTQCGFKFFTREALDEILPHCRLYTEHDVTSRPRVTAFDVE
                     LLYIARRRGLKTAVVPVTWIYGESSKVNPITDTFQNFRDVLMVRWNGWRGRY"
     gene            1950..2306
                     /locus_tag="VFQ54_12740"
     CDS             1950..2306
                     /locus_tag="VFQ54_12740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU0165905.1"
                     /translation="MIETYVYTPCTSCRSTAQYLKARAVDFESRDFFRDRFTRDELAE
                     LLARAGLKPSDILSTRSKPYTALGLAERELGEDELLDLMIEEPRLIKRPLVLGKGTAF
                     VGYSPAKLDGLIAAEG"
BASE COUNT          444 a          690 c          805 g          424 t
ORIGIN      
        1 ggtcggagcg acctacatca tcgccgaggg gccggacggg ctgttcatga ttgaccagca
       61 cgcggcccac gagcgcgtga tgtacgagaa gatccaggcg cagatggtgg agcaatccgt
      121 cgatcgccag ccgatgctcg atccactcgt gatcgagttg ccgcccgacg agcttgtcgc
      181 cttcgagcgg tcggtcgcgg agctgaatgc gatcggcttc gagatcgatc gcttcggcga
      241 gcaatcggtg gcgatccgcg ccgtcccggc gatcgtgaag ggcgtcgata tctccgagcg
      301 gttgcacgac atcctgcagg aactcgttgc cggcggtgcc ggaacctcat ggctggattc
      361 ggtcgcgatc agtgcggcgt gccatacggc gatccgggca gggcaggctc tttcgctgag
      421 cgaaatgcgc gagctcgtgg ctcagctcga gcggacgagt cagccgcgcg cctgcggaca
      481 cgggcgtccg acgatgctgc acatgtcgca gcacgagctc gagcgtcagt tctctcggcg
      541 gtagtcgtcc tccccctttc cggcagcaaa ggggtattcg agagagatca tgtcgagctg
      601 cgctcgacgg gtcagaccag ctggtccgtt acggaagaga cctccagttg acgccgtaga
      661 gggcgcaatc cggaatcgtc gacacccttt gcagagccgg gcttgtaggg ctgaagttaa
      721 tctccgaagg tatcgcataa accgagtggg gcggagaacg ggtggcggtc ggcgctcccg
      781 gtgcgcggtt cgtgctcgtc ccgggtcaat gcggcgcggt cgatcggttt ccgcagcgcg
      841 tgttgcacgg aatggccgtg gtcgggtccg cctgcggcgg atgtgcttcg cacggggatt
      901 tctcgacagg ctcgaaatga cgaaccttga tgcgacagcg ttttcagccg cggggcgaca
      961 tccacgaatc gtattgccgg aagcaggcac cggtccggtc tagtagcgtc cgcgccagcc
     1021 gttccagcgc accatcagga cgtcgcggaa gttctggaag gtgtcggtga tcgggttgac
     1081 cttgctgctc tcgccgtaga tccaggtgac ggggacgacc gccgtcttca gaccgcggcg
     1141 gcgggcgatg tagagcaatt cgacatcgaa tgcggtaaca cgcgggcgcg acgtgacgtc
     1201 gtgttcggtg tagagacggc agtggggaag gatttcgtcg agcgcctcgc gggtgaagaa
     1261 cttgaacccg cactgggtgt cgtggatgcc cggcaggaga aggaacctga ccatcccgtt
     1321 gaagacgcgt cccatcacgt ggcgataggc aggctcgccg atccgcgcgg cgtcggagcc
     1381 ttcgcgagaa gcgatggcga catcgaaacc actgcgaaag gcgtcggcac agcgatccca
     1441 ggtgtcgagt ggcgccgcga gatcgacatc ggtgaatccc gcgatcgggg aattcgagcg
     1501 tgcgagaccg gcgaggaccg ccttcgcctt tcccccgtga ggaagtgcga gcacggtgat
     1561 gttgcgatga ctcgccgcga atgcgttggc gatctcgagg gtgttgtcgg tgctcccgtc
     1621 gtcggcgacg atgatctcgg cggcgatgcg ccgctcggcg aagaatcgcc agaccgtctc
     1681 gagcgtcgcg gcaatcctct cctgctcgtt gtaggcggga agaacgatcg ccacggcagt
     1741 tgaaaccgcg cgtgccgcgc cgctgatctg gtcgctcacc ggggcgagtt ccgaagacga
     1801 gatcttgttc aaccgagtat caccccatgt aagagacaaa cgcctgcccc gacccgccga
     1861 ccgccacatt cataccaact acccggcaaa gtctatcatg agcccgcgcg tattagccgc
     1921 gatttaacgg ttgaagcgga gaaggaacga tgatcgaaac gtacgtttac acgccatgca
     1981 cgagttgccg gagcacggcc cagtacctga aggcgcgcgc cgtcgatttc gagagccggg
     2041 acttcttccg agaccggttc acgcgggacg aacttgccga gcttctggcg cgggcagggc
     2101 tgaagccctc ggatatcctc tcgacccgga gcaagccata cacggcattg ggcctggcag
     2161 aacgagagct cggcgaggac gaactgctgg atctgatgat cgaggagcca agactcatca
     2221 agcgcccgct cgttctgggt aagggcacgg cgttcgtcgg ctacagtccc gcgaagctgg
     2281 atgggctgat cgccgccgaa ggctgagcct tgattgtacg tacatgggtg ttggagaatg
     2341 acgcagcgag aacaggtgtt cgt
//
LOCUS       DASTMD010000857         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1954901, whole genome shotgun sequence.
ACCESSION   DASTMD010000857 DASTMD010000000
VERSION     DASTMD010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954901"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VFQ54_12745"
     CDS             complement(<1..1242)
                     /locus_tag="VFQ54_12745"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEU0165906.1"
                     /translation="MENSTLEKLAAKRLNRRRFAAGATVAGAALATGAVATRAQEATP
                     VSTAVAASPVAGITPVTVASGLNSPRFIAIDGDTVYFTEAGTGGSETFFATPGAGTPA
                     PTDPAGMRGPSGKLSAIGADGTVTAVVSDFTSYTFGADGEIIGASGVALDGNGNAFVA
                     VGAPGPNLPVITRSGTEGVVYKVSLADGSKSIIADLAQYEIDANPDPYAIDSDLYGAA
                     FLDGVVYVADAGGNDVLAVKVDTGDISTFAVTGGLDAPFLPPSGNPLRGGTGTIDSVP
                     SAIVVGPDGKIYVGFVEGGPFPAGLAPIYDYSPDGTKSVFATGLTMIGGLAFSSDGTL
                     YASVMSTDYVNQKPGQVVRVGTDGTLTVVIDNLLVPAGIAFDGADNLFLVHKSTGFPG
                     AGEILKYTGIVAATGTPFVEPA"
     gene            complement(1786..>2953)
                     /locus_tag="VFQ54_12750"
     CDS             complement(1786..>2953)
                     /locus_tag="VFQ54_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEU0165907.1"
                     /translation="AAWVTIAGGKELDEPLAIRPTSESIICPTFARWVQSWRDLPIVM
                     NQWCSVLRWEERPRAFLCTSEFLWQEGHTAHATPEEADERARMMLDVYQRFLEDELAI
                     PVIPGVKSESEKFAGAYRTYTAESMMGGKNWALQSCTSHNLGDHFGRAFDIRFLDKDG
                     ERKYAFSTSWGLSQRTIGAVIMVHGDERGLKLPPKVAPVQAVIVPILGRANPEEVLAR
                     ASELRDALAPTIRVRLDDRDDRSPGFKFNHWELKGVPIRIELGPRDIAAGQAVIAIRD
                     TSEKRTVPLEGLAAAIEDELTAMHARLYAAARDRLVANTRDVATYEELTERVATNAGW
                     NRVWWAGDAADEARMKAETKATIRCIPFEQPGGSGTCVLTGKPATTQVIVARAY"
BASE COUNT          472 a          951 c         1009 g          521 t
ORIGIN      
        1 ggccggctca acgaacggcg tgccggtcgc ggcgacgatg ccggtgtact tcaggatctc
       61 gccggcgccc gggaatcccg tcgacttgtg gacgaggaag aggttgtcgg cgccatcgaa
      121 ggcgatgccg gccggaacca gcaggttgtc gatgacgacg gtaagcgtgc cgtcagttcc
      181 gacgcgaacg acctggcccg gcttctggtt gacgtaatcc gtcgacatca cgctggcgta
      241 gagcgtcccg tccgacgaga aggcgagacc accgatcatc gtgaggccgg tcgcgaagac
      301 cgacttcgtg ccatcggggg agtaatcgta gatcggggcg agaccggccg ggaacgggcc
      361 gccctccacg aagccgacat agatcttgcc gtcggggccg acgacgatgg cgctcgggac
      421 gctgtcgatg gtgccggtgc cgccgcgcag cgggttgccg ctgggcggga ggaacggggc
      481 gtcaaggccg ccggtcaccg cgaaggtcga gatgtcgccg gtgtcgacct tgaccgcgag
      541 cacatcgttg ccgccggcat cggcgacgta gacgacgcca tcgaggaagg ccgcgccgta
      601 gaggtcggaa tcgatcgcat acgggtccgg gttggcatcg atctcgtact gcgcgaggtc
      661 ggcgatgatc gacttgctgc cgtcagcgag gctgaccttg tagacaacgc cttcggtgcc
      721 actgcgggtg atcaccggga ggttcgggcc cggggcaccg acggcgacga acgcgttgcc
      781 gttgccgtcg agcgcgacac cggaggcgcc gatgatctca ccgtccgcac cgaaggtgta
      841 ggaggtgaag tcgctgacga ctgcggtcac tgttccgtcg gcgccgattg ccgatagctt
      901 gccgctcggg ccacgcatgc ccgcgggatc ggtcggggcc ggcgtgccgg cgcccggggt
      961 cgcgaagaac gtctcgctgc cgcccgtgcc cgcttcggtg aagtagaccg tgtcgccgtc
     1021 gatggcgatg aagcgcggag aattcaggcc gctggcgacc gtgaccgggg tgatcccggc
     1081 aaccggcgac gccgcgaccg ccgtgctgac cggggtcgct tcctgggcgc gggtggcaac
     1141 ggcgcccgtc gccagggccg cgccggcgac cgtggcgccg gcggcgaacc ggcggcgatt
     1201 gagccgcttc gcggccagct tctcgagcgt gctgttttcc atcagtgaaa gctccaattc
     1261 gggctacatt gcccagtacg cagggcacgc acttgcgacg agcaccgttt tgcccgtcgt
     1321 cacgcgtccc gcagcattag caccggctgg agacgagact cctccggatg accccaagtc
     1381 gaagtcaagt accagatacg cgcggcactt catctttgga ttgccggttg tctcggctgg
     1441 gcttcggctc ctccatcatt ctagggacca tttgcgcaaa atgtaactac cctttccaag
     1501 cagattctca tatttgttca tcgcatcgtg ccgggtggcg catcatggcc gccaatgccg
     1561 tcacatgccc gtcatcacga cgggatgagg cgatgatttc cccgccgccg ccaacttgcc
     1621 ggaaccgaaa cgccatgcga gcgcaccgcc gaaccggcgg tgcgctcgca tggcgttgag
     1681 atgctgtggg aggcacgacg ggatggcccg agatatttct ggcgcctttc gcgggctcag
     1741 gatgacgacc atgatgtgga gcgtgagtcc acgctccggc ggcggttagt acgcccgcgc
     1801 gacgatgacc tgggtcgttg ctggttttcc ggtgagcaca caggttccgg atccacccgg
     1861 ctgttcgaac gggatgcagc gaatcgtggc cttggtctcg gctttcatgc gcgcctcgtc
     1921 ggccgcgtcg cccgcccacc agacgcggtt ccagcccgca ttcgtcgcga cgcgttcggt
     1981 cagctcctcg tatgttgcga cgtccctggt gttcgcaacg aggcggtcgc gtgccgcggc
     2041 gtaaaggcgc gcgtgcatcg ccgtcagttc gtcctcgatc gcggcggcga gaccctcgag
     2101 cggaaccgtt cgcttctcgc tcgtatcgcg gatcgcaatc acggcctgac cggcggcgat
     2161 gtcgcgagga ccgagttcga tccggatcgg gactcccttc aactcccagt ggttgaactt
     2221 gaatcccggc gagcgatcgt cgcgatcgtc gaggcggacg cgaatcgtcg gcgcgagggc
     2281 gtcacggagt tcgctggccc tggcaaggac ctcctccggg ttggcccgtc ccagaatcgg
     2341 gacgatcacg gcctgaaccg gggcgacctt cggcggcagc ttgaggccgc gctcgtcgcc
     2401 gtgaaccatg atcaccgcgc cgatcgtgcg ctggctgagc ccccatgagg tgctgaaggc
     2461 gtatttccgc tcgccatcct tgtccaggaa gcggatgtcg aaggcgcgtc cgaagtgatc
     2521 gcccaggttg tgggaggtgc aggactggag cgcccagttc ttgccgccca tcatcgattc
     2581 cgccgtgtag gtccggtagg cgccggcgaa cttctcggat tcactcttga cgccggggat
     2641 gaccgggatc gccagctcgt cctcgaggaa tcgctggtag acgtcgagca tcatccgcgc
     2701 gcgctcgtcg gcttcttccg gcgtggcatg ggcagtgtga ccctcctgcc agaggaactc
     2761 gctggtgcag agaaacgccc gcggtcgctc ctcccagcgg agaacgctgc accactggtt
     2821 catcacgatc gggagatcgc gccagctctg cacccagcgg gcgaaggtcg ggcagatgat
     2881 cgattccgat gtcggacgga tcgccagcgg ttcatcgagc tccttgccgc cggcgatcgt
     2941 cacccacgcc gcc
//
LOCUS       DASTMD010000858         1672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_704394, whole genome shotgun sequence.
ACCESSION   DASTMD010000858 DASTMD010000000
VERSION     DASTMD010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704394"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VFQ54_12755"
     CDS             <1..903
                     /locus_tag="VFQ54_12755"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0165908.1"
                     /translation="SEIETLLLYYNKTLFDAQGWTPPKTLDEMMTLAGTINDAGTIPF
                     AHTNAEWKPANEWYIGEFLNHGAGPDKVYQALTGKLQWTDQVFVDALTLLDTCQKNGW
                     FMGGLDRYYTEATADATSALAYGDAAMKIEGTWLVSDANTFFKDSGQEWDWVPMPSTT
                     GDEIYDLGIGSTYSINAKSSRPDDAAEFLNYYFSAEVQAKQVVGINLAPAPVSIPADL
                     LTGDDPRHARMIERLNAASTAGNYGYTTWTFWPPKTETYLIETIEKVWAGDTSLEDYL
                     KGMQEAFDADVKAGTVPPIPARKA"
     gene            997..>1672
                     /locus_tag="VFQ54_12760"
     CDS             997..>1672
                     /locus_tag="VFQ54_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0165909.1"
                     /translation="MVTHVQSAAAATAHFVVEQPRLPFWQRSTWRRKVVPWLFVLPIL
                     LINVVVVLGPALSAIYYSMTEWTGIGSAKWIGLDNYRQLLFHDDSFRHAFKNNLIWLA
                     MFLTVPIALALLAASLLAPLKKGAIVYRMGLFLPYVLPSVVVAAIWRSLFNPDRGIPK
                     TLTDWGIPGFDRALLGDPSTVLPSIAFVDNWHFWGFLMVLFLAAMQNIPADLYEAARL
                     DGASKWQ"
BASE COUNT          299 a          559 c          510 g          304 t
ORIGIN      
        1 agcgaaatcg aaacgctgct gctctactac aacaagacgc tcttcgacgc ccagggctgg
       61 acgccgccga agacgctcga cgagatgatg acgctcgcgg gcacgatcaa cgacgccggg
      121 acgatcccct tcgcgcacac caatgccgag tggaagcccg ccaacgagtg gtacatcggc
      181 gagttcctca accacggcgc cggtccggac aaggtctacc aggccctcac cggcaagctc
      241 cagtggaccg atcaggtctt cgtcgatgcg ctgacgttgc tcgatacctg ccagaagaac
      301 ggctggttca tgggcggtct cgatcgctac tacaccgagg ccacggcgga cgcgacctcc
      361 gcgctcgcct atggcgatgc cgcgatgaag atcgagggga cctggctcgt cagcgacgcc
      421 aacaccttct tcaaggactc cggtcaggag tgggactggg tgccgatgcc gtcgaccacc
      481 ggtgacgaaa tctacgatct cgggatcggc tcgacgtaca gcatcaacgc gaagtcgtcg
      541 cgcccggatg atgccgccga gttcctgaac tactacttct ccgcggaggt gcaggcgaag
      601 caggtcgtcg ggatcaatct cgccccggcg ccggtctcga tcccggccga tctcctcacg
      661 ggggacgatc cgcgccacgc caggatgatc gagcgcctca acgcggcctc gaccgcgggc
      721 aactacggct atacaacctg gaccttctgg ccaccgaaga ccgagaccta cctgatcgag
      781 acgatcgaga aggtctgggc gggcgatacc tcgctcgagg actacctgaa ggggatgcag
      841 gaggccttcg acgccgacgt gaaggcgggc accgtgccgc cgatcccggc gcgcaaggcg
      901 taatcgtcgc tggcaatgtc gccatttcgc cggatccggg cgctccaccc ggatccggtt
      961 ttcgccacgc aatcacccga tccggggagc acgttcatgg tcactcacgt tcaatcagct
     1021 gccgcggcga cggcgcattt cgtcgtcgaa cagccgcggt tgccgttctg gcagcgatcc
     1081 acctggcgcc gcaaggtggt gccgtggctc ttcgtcctgc cgatcctgct catcaatgtc
     1141 gtggtcgtgc tcgggccggc gctctcggca atctattact cgatgacgga gtggaccggg
     1201 atcggttccg cgaaatggat cggcctcgac aattaccggc agctgctctt tcacgacgat
     1261 tcgttccgtc atgccttcaa gaacaacctg atctggctcg caatgttcct gacggtgccg
     1321 atcgcccttg ccctcctcgc ggcaagcctg ctcgcgccgc tgaagaaagg tgcgatcgtc
     1381 tatcggatgg gcctcttcct gccgtatgtc ctgccgagcg tggtcgtcgc ggcgatctgg
     1441 cgatcgctct tcaatcccga tcgcgggatc ccgaagacgc tgaccgactg gggaatcccc
     1501 ggcttcgacc gggcgctgct cggtgatccc agcaccgtcc tcccgtcgat cgccttcgtc
     1561 gacaactggc acttctgggg cttcctgatg gtgctcttcc tcgcggcgat gcagaacatc
     1621 ccggccgatc tctacgaggc ggcgcggctc gatggcgcat cgaagtggca gg
//
LOCUS       DASTMD010000859         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2581761, whole genome shotgun sequence.
ACCESSION   DASTMD010000859 DASTMD010000000
VERSION     DASTMD010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2581761"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFQ54_12765"
     CDS             complement(<1..416)
                     /locus_tag="VFQ54_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165910.1"
                     /translation="MTTQANRILTTHTGSLPRPKDLVTMFRAREKGEPVDPSAMATRI
                     EEAVTEVVAHQRNIGIDVLNDGEASKFAYSTYVKFRLTGFGGDEKRPWGNSALEREFP
                     DLAAKFAVEFGDSVPVRTVCQGPVEYRAEGLEELRT"
     gene            complement(677..>2634)
                     /locus_tag="VFQ54_12770"
     CDS             complement(677..>2634)
                     /locus_tag="VFQ54_12770"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0165911.1"
                     /translation="PTLDWQGFNALVLGDVTSVFFAGRSTVANRRNAFFQAGASARER
                     LKSAAAARWGVTAADVTVASSVLTNTKTGKTLRYGDVAKDAAAVTLAKEPAIKDPSEW
                     TLLGKEDFGKLNNELIVTGKATYGIDVQVDGMKYAAVAQSPVQGGVLKSYDFNTIKDM
                     PGVIKVVEVKATTRHIDKPPILAMNGDMQFINSAVAVVADHYWQAHQALAALKIEWDS
                     GDGAKWATIDQVKKSAFDRIDAGEVKPYQTIGDPAKALAAGKVVSGDYWTPYSEHSMM
                     EPLNGTALVKDDTLEIWMPTSESLQAYSAVQQETGFDPAKITFHPTYVGGSFGRRTNG
                     DDARMVAAVAKGIPGVPVHVIWTREQTFIGGRYRAAVATRAKAVLGEDGIPTVLLLES
                     VAGNQAGWFASPLLQGNPIPNVAVGTAPINTHLLAGPYRGPGYNAAGFILDSFVDELA
                     HAAGIDPLEYRIKLFEPYLDPGYVRALNVAAEKIGWGTTLPKGEGLGIAIANWSQDLQ
                     QKPPFLGSTTATAAHVKVDKDGTLHVLRLEVVPELGTVINHDAVDQQIHGGTIFGLNT
                     AMNEELTLNNGALVETNFDKYPMLRMADMPEINVHFDALTGDPRYSEAGEVPVGPVGP
                     AIANAIFAAIGKRVREMPFRKADLTWL"
BASE COUNT          445 a          841 c          814 g          534 t
ORIGIN      
        1 tcggtcctga gttcctcgag accctcggcc cggtattcga cgggtccctg gcagacggtg
       61 cgaacgggta cggaatcgcc gaactctacc gcgaatttcg ccgccagatc ggggaattcg
      121 cgctcgagcg cggagttccc ccatggtcgc ttctcatcgc cgccgaagcc cgtgagccga
      181 aacttcacgt aggtcgaata ggcaaacttg ctcgcctcgc catcattcag cacgtcgatt
      241 ccgatgttgc gctggtgggc gacgacctcg gtcacggcct cctcgatgcg cgtcgccatc
      301 gccgacgggt cgaccggctc gcccttttcg cgggcgcgga acatggtgac caggtctttc
      361 gggcgcggga ggctgccggt gtgggtggtg agaattcgat ttgcctgcgt cgtcactctc
      421 gtcgtctcct tctgccagtt ctggcatttt tcgttcagtt tttcggatta tactgatcgg
      481 aaaattccaa tcgtgccgcc actatatctg agcgctacgg tgacgccaat aacgagacat
      541 taaagcaacg actgattccg gcccggcaat ggggccgatg tagggtgatg atggaaagct
      601 ggaacgccgg cgggcaacgg gaaacgcccc ttcccagatg ggaaggggcg ttctcgatcc
      661 agtcaacctg tgaatgctac aaccaggtga gatccgcctt gcggaagggc atctcacgaa
      721 cccgcttgcc gatcgcggcg aagatcgcgt tggcgattgc cggcccgaca ggaccaaccg
      781 gcacttcgcc ggcctccgaa tagcggggat ctcccgtcag cgcatcgaaa tggacgttga
      841 tttcgggcat atccgccatg cgcagcatcg ggtatttgtc gaaattcgtc tcgaccaggg
      901 cgccgttgtt gagggtcagt tcctcgttca tcgccgtgtt gagaccgaag atcgtcccac
      961 cgtggatctg ctgatccacg gcatcgtggt tgatgacggt gccgagttcc ggcaccacct
     1021 ccaaccgcag gacgtgcagt gtgccgtcct tgtcgacttt cacatgcgcc gcggtggctg
     1081 tcgtcgagcc aaggaacggc ggcttctgct gcagatcctg cgaccagttg gcgatcgcga
     1141 tgccgagccc ctcgcccttc ggcaaggtcg ttccccagcc gatcttctcg gcggcgacgt
     1201 tcagcgcgcg gacatacccc ggatccaagt acggctcgaa cagcttgatg cggtattcga
     1261 gcggatcgat cccggccgcg tgcgcgagct cgtccacgaa cgaatcgagg atgaagcccg
     1321 cggcgttgta ccccgggcca cgatacggac cggcgagcag gtgcgtgttg atcggcgccg
     1381 taccaaccgc gacgttcggg atcggattgc cctgcagcaa cgggctggca aaccagcccg
     1441 cctggttgcc ggcgacagat tcaagcaaca acaccgtcgg gatgccgtcc tcgcccagca
     1501 ctgccttcgc gcgggtcgcg accgccgcgc ggtagcggcc cccgatgaag gtctgttccc
     1561 gcgtccagat cacatggacg ggcacgcccg ggatcccctt ggcgaccgcc gccaccattc
     1621 gcgcatcgtc accattggtg cgacgaccga agctgccgcc aacgtatgtc gggtggaacg
     1681 tgatcttcgc cgggtcgaat ccggtttcct gctgcaccgc cgaataggct tgcagggatt
     1741 cggatgtggg catccagatc tcgagcgtgt cgtccttgac cagtgccgtg ccattgagcg
     1801 gctccatcat ggagtgctcg gaatagggcg tccagtagtc tccggagacg acctttcccg
     1861 ccgccagcgc cttggccgga tcgccaatcg tctggtacgg cttgacctcg ccggcatcga
     1921 tgcgatcgaa cgcgctcttc ttcacctggt cgatggtggc ccactttgca ccgtcgccgg
     1981 aatcccactc gatcttgagc gcggcgagcg cctggtgcgc ctgccagtaa tgatccgcca
     2041 cgaccgcgac ggcgctgttg atgaactgca tgtcgccgtt catcgccaga attggtggct
     2101 tatcgatgtg cctggtggtc gccttgactt ccaccacctt gatcactccc ggcatgtcct
     2161 tgattgtgtt gaagtcgtaa ctcttcagca cgccgccctg gaccggggac tgcgcgaccg
     2221 cggcgtactt catgccatcg acctggacat cgatcccgta cgtcgccttg ccggtgacga
     2281 tcagttcgtt gttgagcttg ccgaaatctt ccttgccgag cagcgtccat tcggacggat
     2341 ccttgattgc cggctccttg gcgagggtga cagcggcggc gtccttcgcg acatccccat
     2401 agcggagcgt cttgcccgtc ttcgtgttgg tcagcacact gctggcgacg gtgacgtcgg
     2461 cagcggtgac gccccatcgg gcggccgcgg cagacttcag tcgctcacgt gcgctggcgc
     2521 cggcctggaa gaacgcgttg cggcggttgg cgacggtcga tcgaccggca aagaagaccg
     2581 aggtcacatc accgagcact agggcgttga acccctgcca gtcgagtgtc ggcg
//
LOCUS       DASTMD010000860         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3115886, whole genome shotgun sequence.
ACCESSION   DASTMD010000860 DASTMD010000000
VERSION     DASTMD010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3115886"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFQ54_12775"
     CDS             complement(<1..315)
                     /locus_tag="VFQ54_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEU0165912.1"
                     /translation="MSSLATSPKTPQVEVLVFDVTGMDCGDCAKSVERVVGQLPTVGR
                     AEVSFGAGTLTVLPGARRSGSVDDLVRAVGGAVDRAGYTATLRSDLRRGQDGPAWWQN
                     RKV"
     gene            complement(312..761)
                     /locus_tag="VFQ54_12780"
     CDS             complement(312..761)
                     /locus_tag="VFQ54_12780"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0165913.1"
                     /translation="MIETVSPSESDGEETNVVTAIGDGALDDLCDCVHPAAVMEARRA
                     LGDAPPAESMAELFAILGDPTRMRVLTALASGELCVSDLAAATGVNRTTVSHQLSVLR
                     TNRMIRRRREGKVVYYALDDDHVASLLRMASAHAEEELANLDDRRLA"
     gene            complement(1216..>2214)
                     /locus_tag="VFQ54_12785"
     CDS             complement(1216..>2214)
                     /locus_tag="VFQ54_12785"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU0165914.1"
                     /translation="YFAFRGQHFSDIRDAIEAANLWWLIPALVLYFGGVWIRVLRWRI
                     LLHPVVKVSARELYPIVVIGYMANNVLPLRTGEIVRAYTLQRSRNVPMTSSLATIAVE
                     RIFDGLTMLGFMLVATAFVSFTSQLRHLAIVAFIVFTVLVIGLFVLTLGGTFLDRLLQ
                     IVLGPMPTKLADRVEKLVESFLGGLAILRNWKDLTLVAAFSILAWGLEASMYYTLAQG
                     FSKQLRDVMTPAATLLTTGVANMATLIPSSPGYIGQFEYGVKLVVNGALGVNESEALA
                     YAIFVHAALFFPITAWGIFEWSRRQISFKNIQKLAEGDGQAAGAEVASSGSNVSAS"
BASE COUNT          417 a          716 c          733 g          348 t
ORIGIN      
        1 gaccttgcgg ttctgccacc aggcgggacc gtcctgtcct cgccgcagat cgctccgcag
       61 ggtcgcggta tacccggcgc gatcgaccgc gccaccgacg gcacgcacca ggtcatcgac
      121 ggatccggac ctcctcgccc cgggcaggac ggtgagcgtg ccggcgccaa agctcacctc
      181 ggcccgaccg accgttggca gctggccgac gacgcgctcg acgctcttcg cgcaatcgcc
      241 acagtccatc cccgtcacgt cgaagacgag aacctcgacc tgtggtgtct tcggactcgt
      301 cgcgagcgaa ctcatgcgag cctccgatcg tcgagattgg ccagttcctc ctcggcgtgc
      361 gcggaagcca tgcgcaggag cgacgccaca tgatcgtcat cgagcgcgta gtagacgacc
      421 ttgccttcgc gccggcggcg aatcatccgg ttcgtcctga ggacgctcag ttggtgcgaa
      481 accgtcgtcc ggttgacgcc ggtcgccgcc gcaagatcgc tgacacacaa ctcgccggac
      541 gcgagcgcgg tcaggacccg catccgggtc gggtcgccga ggatcgcgaa gagttcagcc
      601 atcgactccg cgggcggggc atcaccgagt gcgcgacgcg cctccatcac ggctgcgggg
      661 tgaacgcaat cgcagaggtc gtccagcgcc ccgtccccga tcgcggtcac cacgttcgtc
      721 tcttcgccat cggattcaga gggagataca gtctcaatca tgtgcgcctc catacactcg
      781 tatcaatggc tggagtctag cactgcatgt gtgcacaagc aagcacacgt cgtaggcccg
      841 catctcatcc tgagtctggg gtgggtcacg gtcaaccatc gtgtgaggat gaatcggcgc
      901 cgccgattcg aggcgaccag ctcggtcgcc aggagggaaa tgggaggggc gagagcgaga
      961 gttcatcact cgccgcaagc ccaacatcaa cgctcggcgc cgtgcatcaa ggggagaaga
     1021 gcgcgcacga ggctcgcccc tacggcgccc tgggagcgtc gcaggtgctg tcggatcgga
     1081 ccaggcgttg cgaccggcat ccggtcgttc gtagggcgga accgccgctg ccggaccgga
     1141 ggtccggacg gcctgtcccg cctctcgctc acccaggcgt cgtgcttccg gtggatgccg
     1201 ttccagcctc tcgctctagg aggccgagac gttggagccg gaggacgcga cctcggcccc
     1261 ggctgcctga ccgtcgccct cggcgagctt ctggatgttc ttgaacgaga tctgccggcg
     1321 cgaccactcg aagatacccc aggccgtgat cgggaagaag agcgccgcgt gcacgaagat
     1381 cgcgtacgcc aatgcctcgc tctcgttgac cccgagcgcc ccgttcacca cgagcttcac
     1441 gccgtactcg aactgcccga tgtagcccgg cgaggacggg atcagcgtcg ccatgttcgc
     1501 gactccggtc gtcagcagcg tggcggcggg cgtcatcaca tcgcgaagtt gcttgctgaa
     1561 tccctgcgcc agcgtgtagt acatcgacgc ctcgagtccc caggcgagga tcgagaacgc
     1621 ggcgaccagg gtcaggtcct tccagttcct caggatcgcg aggccgccca gaaatgactc
     1681 gacgagcttc tcgacgcgat ccgccagttt cgtcggcatc gggccgagca cgatctgaag
     1741 cagccggtcg aggaatgtcc caccgagcgt gaggacgaag agtccgatca caagcacggt
     1801 gaagacgatg aaggcgacga tcgcgaggtg gcgtaactgg ctcgtgaacg agacgaacgc
     1861 ggtcgccacc agcatgaagc cgagcatcgt caggccatcg aagatgcgct cgacggcgat
     1921 ggtggccagc gaggaggtca tcggcacgtt gcggctgcgc tggagggtgt aggcgcggac
     1981 gatctcgccc gtgcggaggg gaaggacgtt gttggccatg tagccaatga caacgatggg
     2041 atagagctcg cgggcggaga ccttgacgac gggatggaga aggatccgcc agcggagaac
     2101 gcgaatccag acgcctccga agtagaggac gagcgcagga atcaaccacc acaggttggc
     2161 ggcttcgatg gcatcgcgga tgtcgctgaa atgctgcccg cggaaggcga agta
//
LOCUS       DASTMD010000861         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2314486, whole genome shotgun sequence.
ACCESSION   DASTMD010000861 DASTMD010000000
VERSION     DASTMD010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2314486"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFQ54_12790"
     CDS             complement(<1..647)
                     /locus_tag="VFQ54_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165915.1"
                     /translation="MATASWETKFSRSELGPLSVVAEGLAFPSSLAFDDDGNAYVGET
                     GIGFGGSSRGGKVYKIGPNGEKELIVEGLGHPLTGLTWHDGSLWVSQGSNPGRITRVE
                     LDGTWKDVLNNLPGGGNYHTGEVAFGPDGKMYWGQGAMTNTGLVGLDALIMGWLKKLP
                     HSVDIPGYDIVLNDVRVETSNPFKKDDPDARLVTGPFSQFGETVEQGTVIKGQTPA"
     gene            complement(680..1408)
                     /locus_tag="VFQ54_12795"
     CDS             complement(680..1408)
                     /locus_tag="VFQ54_12795"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU0165916.1"
                     /translation="MKIKRGDLPHDDVGAGCDVIRRYALIALGIAIFVLASFLLVEAL
                     HIPLLVDPSNHLEKGGFGAAALGVGLLIVDVVLPVPSSLVMTAQGALFGIVVGTILSM
                     IGSIGAAMVGYGIGRKSTRLIARFVTPEEQAQSNRLINRWGPLALIVTRPVPVLAETT
                     AIMAGTSSLTWRQVLIGVTIGAFPASLIYAIAGAYATSVASGLLVFVLVIVLAGAFWA
                     IGWYIEKRFLLPRRNTAVARQDRH"
     gene            complement(1568..2596)
                     /locus_tag="VFQ54_12800"
     CDS             complement(1568..2596)
                     /locus_tag="VFQ54_12800"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0165917.1"
                     /translation="MTQWSDKMLLGIDTFSLRSQGWNAFQMLDYAAGLALQNVHFSER
                     ANLESHDDDYLRELRRYADERGIQIEVGMRSINTYAETFDASLGSGEQQLADMLVAAK
                     KLGSPVVRCFVGMQNDRLGAVPFAQIVDETIRTLKAAAPAAEQAGIAIAVENHGGVDL
                     LARELRAIVEEVGSDFVGVCLDTGNPLYGGEDAVLATEILAPYVKTAHVRDTRVWAIE
                     HGAAVQWAPAGQGDIDLRRIVEILNATTPDIALDLEIISGGGWKEINFRNPDDEFWSY
                     YPGMLASDFIRFENLLDGGSTGPLDQYVPDGPFAELSDEERERYLAQQRHQFEISVAY
                     CHDHLGLA"
     gene            complement(2677..>3251)
                     /locus_tag="VFQ54_12805"
     CDS             complement(2677..>3251)
                     /locus_tag="VFQ54_12805"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0165918.1"
                     /translation="SSHAVDLARFLAGEIATISALTPRFVPERMGPDNTPIRIEWDDA
                     TLSLVEFASGAVGTIEASRVATGRRNSLRMEINGSRGSIGFDVERLNELQVSLADALV
                     EDVVGTQDVMVTESTHPFAKVWWPAGHVLGWEHAHINEIQHLLRAIVDDTDVAPLGAT
                     FVDGYRVAVIADAIERAAASGQKQEIIYED"
BASE COUNT          582 a         1021 c         1057 g          591 t
ORIGIN      
        1 gccggcgtct ggcccttgat caccgtgccc tgctcgacgg tctcgccgaa ctgcgagaac
       61 ggaccggtga cgaggcgggc gtcgggatcg tccttcttga agggattgct cgtctcgaca
      121 cggacatcgt tgaggacgat gtcgtagccg gggatgtcga cgctgtgcgg gagcttcttg
      181 agccagccca tgatgagggc atcgagaccg acgagaccgg tgttggtcat cgcgccctga
      241 ccccagtaca tcttgccgtc gggtccgaag gcgacttcac cggtgtgata gttgccgccg
      301 cccggcaggt tgttcaggac gtccttccag gtgccatcga gttcgacgcg ggtgatgcga
      361 cccgggttgc taccctggga gacccagagc gagccgtcat gccaggtgag accggtcagc
      421 gggtggccga gtccctcgac gatgagttcc ttctcgccat tcgggccgat cttgtagacc
      481 ttgccgccgc gggaagagcc gccgaaaccg atgccggtct cgccgacata ggcgttgccg
      541 tcgtcatcga atgccaagct gctggggaag gcgaggcctt ccgcaacgac cgagagtggc
      601 ccgagttccg agcgcgagaa ctttgtttcc caggatgccg tggccatgac ctgaaccttt
      661 cctcgttgaa atgtgacgtc tagtgccgat cctgccgggc aactgccgtg ttgcgccggg
      721 gcagcaggaa ccgcttctcg atataccagc cgatagccca gaacgctcca gcgagaacga
      781 tcaccagaac gaaaacgagc aaaccactcg cgacgctcgt ggcgtaggct cccgcgatcg
      841 catagatcaa cgatgccggg aacgccccga tcgtgacgcc gatcagcacc tgtcgccagg
      901 tgagactcga cgtccccgcc atgatcgccg tcgtctccgc gagaaccggc accggtcggg
      961 tcacgatcaa ggccagggga ccccagcgat tgatgaggcg attcgactgt gcctgttctt
     1021 ccggcgtgac gaaccgtgcg atgagccggg tgctcttgcg accgattccg tagccgacca
     1081 tcgccgcgcc gatgcttccg atcatcgaga ggatcgttcc gacgacgatg ccgaagagcg
     1141 ccccctgggc cgtcatgacg aggctcgagg gaaccggcag cacgacgtcg acgatcagca
     1201 acccgacgcc gagcgccgcc gccccgaagc cacctttctc gaggtgattc gagggatcga
     1261 cgagcaacgg aatgtgcagc gcctcaacga gcagaaagct cgcgagcacg aagatcgcaa
     1321 ttcccagcgc gatgagcgca tatcgtcgta tcacgtcgca gccggcgccc acgtcatcgt
     1381 gcggcagatc tccacgcttg atcttcatcc cggaggtcga tggcctatcg actgatcaat
     1441 cgtgagttta ctcagaatct gagcagagta aataggccac atgtgtcgat tttctgaggt
     1501 acttcatcaa gtattgacgt ggatcgtcac gccggaaagg ttgagcggcg ctcggccttc
     1561 cccggggcta cgccaggccg agatgatcgt ggcagtaagc gacgctgatc tcgaactgat
     1621 ggcgctgctg cgcgagatag cgctcgcgtt cctcgtcgga aagttccgca aagggcccgt
     1681 ccgggacgta ctggtcgagc gggccggtcg agccgccgtc gagcaggttc tcgaagcgga
     1741 tgaaatcgct cgccagcatt cccgggtagt acgaccagaa ctcgtcgtcc gggttccgga
     1801 agttgatctc cttccagccg ccaccgctga tgatctcgag atccagcgcg atgtccggtg
     1861 tcgtcgcgtt caggatctcg acgatgcgcc tgagatcgat gtcgccctgg ccggccggcg
     1921 cccactggac ggcggcgcca tgctcgatcg cccagacgcg ggtgtcgcgg acgtgggcgg
     1981 tcttgacgta gggcgcgagg atctcggttg cgagcacggc atcctcgccg ccgtagagcg
     2041 gatttccggt atcgagacag acgccgacga agtcggaccc gacttcctcg acgatcgcgc
     2101 ggagttcccg cgcgaggaga tcgacgccgc cgtggttctc gacggcgatc gcgattcctg
     2161 cctgctcggc cgccggtgcc gccgccttca gcgtgcgaat cgtctcgtcg acgatctgcg
     2221 cgaagggaac ggcgccgagg cgatcgttct gcatgccgac gaaacagcga acgaccggcg
     2281 agccgagctt cttcgcggcg accagcatgt cggcgagctg ctgctcgcca ctcccgagcg
     2341 aggcatcgaa ggtctccgca tacgtgttga tgctgcgcat cccgacttcg atctggatgc
     2401 cacgctcgtc ggcgtagcgc ctgagttcac ggagatagtc gtcatcgtgc gattcgagat
     2461 tggcgcgctc cgagaaatgg acgttctgca gggcgagccc ggcggcgtaa tccagcatct
     2521 ggaacgcgtt ccagccctgc gatctgagac tgaaggtatc gattccgagc agcatcttgt
     2581 cactccactg ggtcatatgc gtgtttcgtc ccggcggggc atgtccccgc ctccgggagt
     2641 gaggcgacca catggggcgc cgggacggga ctccggctag tcttcgtaga tgatctcctg
     2701 tttctgaccg gatgccgccg cccgttcgat cgcgtcggcg atcacggcga cacgatagcc
     2761 atcgacgaag gtcgcgccga ggggagcgac atcggtgtca tcgacgatcg cgcgcagcag
     2821 gtgctggatc tcgttgatgt gcgcgtgttc ccagccaagg acgtgaccgg ctggccacca
     2881 gaccttggcg aagggatggg tcgattctgt caccatcacg tcctgcgtgc cgacgacatc
     2941 ctcgacgagg gcgtcggcga gactgacctg gagctcgttc agccgctcga catcgaagcc
     3001 gatgctgccg cgggagccgt tgatctccat tcgcagcgag ttgcgccggc cggtcgcgac
     3061 gcgggaggct tcgatggtgc cgaccgcgcc gctcgcgaac tcgacgagcg agagcgtcgc
     3121 atcgtcccac tcgatcctga tcggcgtgtt gtccggtccc atccgctcgg gaacgaagcg
     3181 cggagtcagg gcgctgatcg tggcgatttc gccagcgagg aaacgagcca agtcgacggc
     3241 gtggctgctg a
//
LOCUS       DASTMD010000862         4191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2582025, whole genome shotgun sequence.
ACCESSION   DASTMD010000862 DASTMD010000000
VERSION     DASTMD010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582025"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VFQ54_12810"
     CDS             complement(<1..935)
                     /locus_tag="VFQ54_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU0165919.1"
                     /translation="MPKSIRALCILAALTLVGVGLFTSHELSDPRWLTLLGGSWILLL
                     IGTRITLPAETPTVGRAMVRTAMVLTTIFAVIAAQLARVQVVQRSATYNKVETTKDGF
                     TFSNPRKTGTSLTEQRGRIYDRNGELIADTQKDGDIYKRVYPNPVTAYVAGYYSPLLY
                     GADGLEETYSQQLTGQSEDDSAEKLIDGLLHRDGVGLDLHLTLDSGLQQTATDQLQGR
                     KGAAVVMEIETGAILVLASNPNYDPNKLFTTSSSESVSGPAGDYWESLNKDPDAPFVL
                     RPNLGLYTPGSTFKTVTASAAIDSGLAKPDDIYQD"
     gene            complement(969..2285)
                     /locus_tag="VFQ54_12815"
     CDS             complement(969..2285)
                     /locus_tag="VFQ54_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU0165920.1"
                     /translation="MQMRQHPAFRGTEISLLLLPSLLAIIGLITIVVVPRHKVSWSWG
                     DIWVSIAFASALIGISLTFSIRRFRGDQVLLPLCSVLAMLGLLLIQRLSSDLTAQDPT
                     YATLAQKQFIYMAAGLAMMWLIAMLGGPLRLIEWLPRYKYTWLLLSLALQAATFVVGV
                     DVGGARLWIQVGPVQIQPSEIIKITLVIFLAGFLDEKRDLIGSSWNIGPLALPPIPYL
                     LPMIAMWGAALLTLVVLNDLGSALLFFGVFMVMLYVTSGKAIYVVVGTIAFAVACYLA
                     WLAFSRIDIRVQNWLDPWKDAVNGFQPVQSDYSLASGGVLGTGLGMGDPTLIPAVATD
                     YVFSAIGEELGLLGTLGIVALSFLIMIRGYLIALRARDGFAQLLATGLTTIVAIQTII
                     IIGGVIRLIPLTGITLPFISYGGSSTICNFVILGLLLYISSLPRRA"
     gene            complement(2306..3166)
                     /gene="mtnP"
                     /locus_tag="VFQ54_12820"
     CDS             complement(2306..3166)
                     /gene="mtnP"
                     /locus_tag="VFQ54_12820"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEU0165921.1"
                     /translation="MSMLGIIGGSGLYAMPGLEEIEEQNVETAYGPPSSPIVVGSLEG
                     VKVAFLARHGRGHFLNPSEVPYRANIAAFRRIGVTHLLSLSAVGSLKQELPPRTAVLP
                     DQIIDITRQRARTFFEGGIVAHVGIADPFCTTLRAAVAPIAAAASPSSQDGGTYVCIE
                     GPQFSTRAESRLYRSWGADIIGMTAMPEARLAREAGLCYATVAMVTDYDVWHESEDDV
                     TVEVVQRVMADNVAANHEIVRGLAKRGLPACECGCASAIANAIITAPAAMSDAGRATL
                     ARFAAREDVI"
     gene            3427..3696
                     /gene="rpsO"
                     /locus_tag="VFQ54_12825"
     CDS             3427..3696
                     /gene="rpsO"
                     /locus_tag="VFQ54_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU0165922.1"
                     /translation="MALQPAQKEEIISAHRLHEGDTGSPEVQIALLTERINGLTAHLR
                     LHRQDHHSRRGLLKLVGRRRRLLAYLSNEDIERYRATIARHGLRR"
     gene            4011..>4191
                     /locus_tag="VFQ54_12830"
     CDS             4011..>4191
                     /locus_tag="VFQ54_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165923.1"
                     /translation="MIPEYVQEKIKTVTVEIGGRTLSIETGRLAEQAMGAVVVRFGES
                     IVLSTVVGDKKPKEDL"
BASE COUNT          822 a         1222 c         1416 g          731 t
ORIGIN      
        1 ttgtcctggt agatgtcgtc gggtttggcg aggccggagt cgatcgcggc ggatgccgtc
       61 acggtcttga aggtcgaccc cggtgtgtag agaccgagat tggggcgcag cacgaaggga
      121 gcatcggggt ccttgttgag cgactcccag taatcgcccg caggtccgga gaccgactcg
      181 gaggacgagg tcgtgaagag cttgttcgga tcgtagttgg gattgctggc gaggacgagg
      241 atcgcccccg tctcgatctc catcacgacc gcggcgccct tccgtccctg gagttggtcg
      301 gtcgcggttt gttgcagtcc cgagtcgagc gtgaggtgaa gatcgaggcc gacgccgtcg
      361 cggtggagaa gaccgtcgat cagcttctcc gctgaatcgt cctccgattg tccggtcagc
      421 tgctggctgt atgtctcctc gagtccatcg gcgccataga ggagcggaga gtaatagccg
      481 gcgacgtacg cggtgacggg attgggatag acgcgcttgt agatatcacc gtccttctgc
      541 gtatccgcga tgagctcgcc gttgcgatcg tagatcctgc cacgttgttc ggtcagactc
      601 gtgccggtct tgcgcgggtt cgagaaggtg aagccgtcct tggtcgtctc gaccttgttg
      661 tacgtcgcgc tccgctggac gacctgaacg cgggcgagtt gggcggcgat gacggcgaag
      721 atcgtcgtca ggaccatcgc cgtgcgcacc atcgcccggc cgacggtcgg cgtctccgcc
      781 gggagcgtga ttcgggtgcc gatcaggagc agaatccagg atccgccgag cagggtcagc
      841 cagcgcgggt cggagagttc atgactcgtg aagagtccga cgccgacgag cgtgagtgcg
      901 gccagaatgc agagggcacg aatcgatttc ggcatgcgca gggcgactcc gtggcgataa
      961 cgcgcggatc aggcgcgacg cggcaggctc gagatgtaaa gcagaagtcc gagaatgacg
     1021 aagttgcaga tcgtggagct tccgccgtac gagatgaacg gcagggtgat tcctgtcagc
     1081 gggatcaacc ggatcacacc gccgatgatg atgatcgtct ggatcgcgac gatcgtcgtc
     1141 aggccggtcg ccagcaattg cgcgaagccg tcccgggctc gaagcgcgat cagatagccg
     1201 cggatcatga tcaggaacga gagggcgacg atgcccagtg tcccgagcag gccaagctct
     1261 tcgccgatcg ccgagaagac ataatcggtc gcaacggcgg gaatcagcgt cggatcgccc
     1321 attcccaatc cggtgccgag cacgccaccg ctggcgagcg agtagtccga ttgaaccggc
     1381 tggaagccat tcacggcgtc cttccacgga tcgagccagt tctggacgcg gatatcgatc
     1441 cgggagaagg caagccatgc caggtaacag gcgacggcaa acgcgatcgt cccgacgacg
     1501 acgtagattg ccttcccgct cgtgacgtag agcatgacca tgaagacgcc gaagaagagc
     1561 agtgcagaac cgagatcgtt caggacgacc agggtcagga gcgcggctcc ccacatcgcg
     1621 atcatgggca ggagataggg gatcggcggc agcgcgaggg ggccgatgtt ccagctggag
     1681 ccgatcaggt cgcgcttctc gtcgaggaag ccggcgagga agatcaccag tgtgatcttg
     1741 atgatctccg acggctggat ctggaccgga ccgacctgga tccagaggcg ggcgccgcct
     1801 acatcgacgc cgacgacgaa tgtcgcggcc tggagcgcga ggctcaggag cagccaggtg
     1861 tacttgtagc gcggcaacca ttcgatcagc cgcaacggtc ccccgagcat cgcgatcagc
     1921 cacatcatcg ccagcccggc cgccatatag atgaactgct tctgggcgag cgtggcgtag
     1981 gtgggatcct gggcggtgag gtcggaggag agacgctgga tcaagagcaa gccgagcatc
     2041 gccaggacgg aacacagggg cagcaggacc tggtcgccgc gaaacctccg gatgctgaac
     2101 gtcaggctga tcccgatgag ggcgctcgcg aaggcgatgc tgacccagat gtctccccac
     2161 gaccaggaga ccttgtgccg gggaacgacg acgatcgtga tgagcccgat gatggcgagc
     2221 agggacggca agaggaggag agaaatctcc gtcccacgga atgccgggtg ttgcctcatc
     2281 tgcatgaagg atgctcccga ttgacctaga tgacatcctc acgcgcagcg aagcgggcga
     2341 gcgtcgcgcg gccggcgtcg ctcattgcag ccggggcggt gattatcgcg ttggcgatcg
     2401 cggaggcgca gccgcattcg catgccggga gtccccgctt cgcgagcccg cgcacgatct
     2461 cgtggttggc ggcgacgttg tcggccatca cgcgctggac gacctcgacc gtgacgtcgt
     2521 cctcgctttc gtgccagaca tcatagtcgg tgaccatggc gacggtcgca tagcagagac
     2581 ctgcctcccg ggcgagccgg gcctccggca tcgccgtcat cccgatgatg tcggcgcccc
     2641 aggagcgata aagccgcgac tccgcgcgag tcgagaattg cggaccctcg atgcagacgt
     2701 acgtcccgcc gtcctgggac gacggtgaag ccgcggccgc gatcggcgcg acggcggcgc
     2761 gcagggtcgt acagaaggga tcggcgattc cgacgtgagc gacgatgccg ccctcgaaga
     2821 acgtacgtgc gcgctggcgg gttatatcta tgatctgatc cgggagcacc gcggtgcggg
     2881 gagggagttc ctgtttcagg ctgccgaccg cgctcagcga aagaaggtgg gtgacaccga
     2941 tgcggcggaa cgcggcgata ttggcgcgat acggcacttc ggatggattg aggaaatgcc
     3001 cgcgcccgtg gcgggcgaga aacgcgacct tgacgccctc gaggctaccg acaacgatcg
     3061 gggaactcgg cgggccatag gcggtctcca cgttctgctc ttcgatctct tcgagaccgg
     3121 gcatggcgta gagtccactg cctccgatga ttccgagcat ggacataagg ttgtcatcct
     3181 tgcgaactgg cacgagggaa gcacgaagat ggcacccggt gccatccgaa agatgtcgat
     3241 ttcatggtac cgtcgaggtg atacgtccaa caatcagcct tgggcggggt caagcgtgcg
     3301 ctttgtgaaa aaagcggggt gcgtgctata ctcggctcgt caagcgccat cgccacgggc
     3361 gttttttcca tcctgcacgt cgctacaaat gtcgaccgat caacggtccg aggaggcttc
     3421 gccggcatgg cgctgcaacc agcacaaaaa gaagagatca tttcggctca ccgtctccac
     3481 gagggagaca cgggctcgcc ggaagtgcag attgcgcttc tgaccgagcg catcaacggt
     3541 ctgaccgcac acctccggct gcaccggcag gaccaccact cccgacgtgg gctgctgaag
     3601 ctcgtcggtc gtcgccgtcg cctgctggct tatctgagca acgaggatat cgagcgatat
     3661 cgtgccacga tcgcgcgtca cggtcttcgc aggtagcccc tggacgtcgc gtccaggtgt
     3721 tgctcaaggc catcctcgcg gtgatatgtg cacgttgcac gatggaccaa agcccaggac
     3781 cgtctggtcc tgggctttgc gcgtcgtcgc gagcatgaaa ggaggaaggt ttctccttac
     3841 ggctgcctga tcttggcggt tggttgaggt attggagcgt ggggagatcg ctcctcgcaa
     3901 ggtccccacg ctccagttcc tcaatcctcc gggcaggcgc catcttcacg aatgatgaat
     3961 gcatcgggag cgggcgaaga cccgcggatc ccgaatggcc tggaggtcac atgattccgg
     4021 aatacgttca agagaagatc aagacagtca ccgttgagat cggtggacgc acactttcca
     4081 tcgagaccgg tcgcctcgca gagcaggcga tgggcgccgt cgtcgtccgg ttcggcgagt
     4141 cgatcgtgct cagcacggtc gttggcgaca agaagccgaa agaagatctc c
//
LOCUS       DASTMD010000863         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1240345, whole genome shotgun sequence.
ACCESSION   DASTMD010000863 DASTMD010000000
VERSION     DASTMD010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240345"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..595)
                     /locus_tag="VFQ54_12835"
     CDS             complement(2..595)
                     /locus_tag="VFQ54_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU0165924.1"
                     /translation="MVFPLIGARDYIEQRGFYIHECPSCKAPAIFAVYETRRKMTLYF
                     VPTVPVRSQYVMECMTCHNKWGVPKEQLNSLLSSLMTQEELAERTREAQRRAQMLANG
                     QMPLGAVGGPPTLYEVLQVHYNAEPEVVEAAFKRLALKYHPDRSKDPDAPARMRALIT
                     ARDILGNEEKRAVYDRSLGIVRQKRVIRTEALRADEV"
     gene            802..1494
                     /locus_tag="VFQ54_12840"
     CDS             802..1494
                     /locus_tag="VFQ54_12840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEU0165925.1"
                     /translation="MATRSITPTFVRKWLETLHDAELAQVAPDPARTAIFSADMINGF
                     LREGPLSSERVNQLTEPVLALFTAAYDHGVRDFVLSQDTHSEETPEFRSYPPHALKGS
                     SESRTIPEIEALPFVEHMTIVEKNSLNPAIGTRFDAWLEQHPDLQTAIVVGDCTDLCV
                     YQLAMHLRLRANALDLMKFDVIVPANCVETFDIPSADAQGPGIAHPGDFFHEVFLYHM
                     ASNGIRVVKSLQ"
     gene            1520..2965
                     /locus_tag="VFQ54_12845"
     CDS             1520..2965
                     /locus_tag="VFQ54_12845"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU0165926.1"
                     /translation="MPTVRSGLITEERERRYIADPTITPGPTALFTDMYHVDATYVAW
                     KTGQTGTATFDLYTRTNPFGGGFMLVAGLEPALDYLRAFKYSWQDREYLQATKGYEPE
                     FLDYLRDIQFTGEIQAIPEGEIAFPNEPLLRITAPFAEALLIESGLLRSVGISTLIAT
                     KAARLTLAAKGRGLADFAFRRAHDPFLAARSGYIGGLASTSFVAAAKAYGIPTSGTIP
                     HALVQAYPDELTAFRAVAATLPEYSLLLDTYDVTTGIGHAIVAGKEAQAEFGHHLVAV
                     RLDSGDLDADSRMVRTRLDEAGMQDVRVLVSGDLDEWKVQALVDAGAPVDGFGVGGNL
                     GVGLGSIESGTVGGVIGAVYKLASYGGDADEAAAARIKLAGGGDKSTWPGRKVPYRIG
                     TFTRDVVALEEEAPPTGGRSLLQPLIRDGHLINDVPSIAEARARARASLDALPERLRS
                     LAVPREERYEVEMSAALRDLRERTIAGISAD"
BASE COUNT          512 a         1028 c          944 g          494 t
ORIGIN      
        1 cctacacctc gtcggcgcgg agggcttccg tgcggatcac gcgcttctgg cggacgatgc
       61 caagcgagcg gtcgtagacg gcgcgcttct cctcgttccc gaggatgtcg cgcgcggtga
      121 tcagcgcgcg catccgggcg ggggcgtcgg ggtccttcga gcgatccgga tggtacttca
      181 gggcgagccg cttgaacgcc gcctcgacca cctccggctc ggcgttgtag tggacctgca
      241 gcacttcgta gagcgtcggt gggccaccga ctgcgccgag cggcatctgc ccgttcgcca
      301 gcatctgcgc gcggcgctgc gcctcgcgcg tgcgctccgc aagctcttcc tgcgtcatca
      361 ggctcgagag caggctgttc agctgttcct tcggcacgcc ccatttgttg tggcaggtca
      421 tgcattccat gacgtactgg gagcgcacgg gaacggtcgg aacgaaatag agcgtcatct
      481 tgcgacgcgt ttcatagacg gcaaaaatcg ctggcgcttt gcaggatggg cattcgtgaa
      541 tgtagaagcc gcgttgctcg atgtaatcgc gagcgccgat aagcgggaag accactggtg
      601 gaggaccttc gtcgaaaact gctagaggct cttctctctg actgtaccat gcgccgacac
      661 tccgaacatc tccgatgcgg cgaacgccca ggtcactgca cgagcggata cactccaata
      721 ggcacgcccc ggccggagcc ctcgaaaaga gggtgaggac cgatgccgat atctcgtatt
      781 tccgccgccc ggaggatcct catggcgacc cgctcgatta ccccgacgtt cgtccgcaag
      841 tggctggaaa cgctgcacga tgccgagctg gcgcaggtcg ccccggatcc ggcgcgcacg
      901 gcgatcttct cggcggacat gatcaacggc ttcctgcgcg aaggcccgct ctcgtcggag
      961 cgcgtcaacc agctcaccga accggtgctc gccctcttca ccgccgcata cgaccacggc
     1021 gtccgggact tcgtcctctc gcaggacacc cacagcgagg aaacgcccga gttccgctcc
     1081 tacccgccgc acgccctgaa gggaagcagc gagtcacgga cgatccccga gatcgaggcg
     1141 ctgcccttcg tcgagcacat gacgatcgtc gagaagaact cgctcaatcc ggcgatcggc
     1201 acccgcttcg atgcctggct cgaacagcac ccggacctgc agaccgcgat cgtcgtcggc
     1261 gattgcaccg atctctgcgt ctaccagctc gcgatgcatc tgcggctccg cgccaacgcc
     1321 ctcgacctca tgaagttcga tgtgatcgtg cctgccaact gcgtcgagac gttcgacatc
     1381 ccgtccgcgg atgcccaggg tccgggaatc gcccacccgg gagacttctt tcacgaagtc
     1441 ttcctctatc acatggcgag caatggaatc cgcgtcgtga agtcgctgca atagaacgcc
     1501 accaggaagg gtcccgcgga tgccgacagt tcgaagcggt ctgatcacgg aagaacgtga
     1561 gcgacgatac atcgcggatc cgacaatcac gccgggaccg accgccctct tcaccgacat
     1621 gtatcacgtc gacgcgacct acgtcgcctg gaagaccggg cagaccggga ccgcgacatt
     1681 cgacctctac acgcggacga atccgttcgg cggcggcttc atgctcgtcg ccgggctcga
     1741 gccggccctt gactacctcc gcgccttcaa gtactcgtgg caggaccgcg agtatctcca
     1801 ggcgaccaag ggctacgagc cggaattcct cgactacctg cgcgatatcc agttcaccgg
     1861 cgagatccag gcgatccccg aaggcgaaat cgccttcccg aacgaaccgc tcctgcggat
     1921 caccgctccc ttcgcggagg cgctgctgat cgaatccggg ctcctccgct cggtggggat
     1981 ctcgacgctg atcgcgacga aggccgcgcg tctcaccctc gccgcgaagg gacgcgggct
     2041 cgccgacttc gcctttcgcc gcgcccacga tccgtttctc gcggctcgtt ccggctacat
     2101 cggcggtctc gccagcacgt cgttcgtggc ggcagcgaag gcctacggca tccccacctc
     2161 gggcacgatc ccgcacgcgc tcgtccaggc atacccggat gagctgaccg ccttccgcgc
     2221 cgtcgccgcg accctgccgg agtattccct gctgctggac acctacgatg tgacgacggg
     2281 gatcggccac gcgatcgtcg ccggcaagga ggcccaggcc gaattcggac accatctcgt
     2341 cgccgtccgt ctcgacagcg gcgatctcga cgccgacagc cggatggttc gcacccggct
     2401 cgacgaggcc gggatgcagg atgtccgggt gctggtcagc ggtgatctcg atgaatggaa
     2461 ggtccaggca ctcgtcgacg ccggcgcgcc ggtcgacggg ttcggcgtcg gcggcaacct
     2521 cggggtcggt ctcggctcga tcgagagcgg gaccgtcggc ggcgtgatcg gcgcggtcta
     2581 caagctcgcc tcctatggcg gcgacgccga tgaggcggcc gcggcgcgaa tcaagctcgc
     2641 gggtgggggc gacaagagca cctggccggg gcgcaaggtg ccctaccgga tcgggacgtt
     2701 cacgcgggat gtcgtcgcgc tcgaggaaga ggcgccgcca accggtggac gctcgctgct
     2761 tcagccgctg atccgcgatg ggcatctgat caacgatgtc ccgtcgatcg cggaagcgcg
     2821 cgcccgcgcc cgggcatcac tcgatgcgct gccggagcgg ctacgatcgc tcgccgtccc
     2881 ccgcgaagaa cggtacgagg tggagatgag cgccgcgctg cgcgacctcc gcgagcgcac
     2941 gatcgccggc atctccgcgg actaaagggc gcgtccac
//
LOCUS       DASTMD010000864         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_972983, whole genome shotgun sequence.
ACCESSION   DASTMD010000864 DASTMD010000000
VERSION     DASTMD010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972983"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VFQ54_12850"
     CDS             <1..800
                     /locus_tag="VFQ54_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0165927.1"
                     /translation="NGAGKSTTLRTLLGFLRPTSGRAEILGMDSHAEAVAIHRRLGNL
                     PSEFNLEDRMTGEQLLRYFADLRGIDRTLGYAHELAARLDADLTRPMRRLSRGNKQKI
                     GIIQALFHRPDVIVLDEPTSGLDPLVQEEFLTILEEIRALGQTIFFSSHILPEIERIA
                     DRVGIIRAGELVAVEQPSALTARAFRYVRIRFAEPLSSSGREELAGLPGVEKFAADGS
                     TVTFTAHGATDAIVKFAATREVEQFDSERPPLEEIFLTYYAEAPDGR"
     gene            916..>2021
                     /locus_tag="VFQ54_12855"
     CDS             916..>2021
                     /locus_tag="VFQ54_12855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165928.1"
                     /translation="MKSSSPELTPSGTAPQFVVPLYPRVVVLGISQILAWGATFNLPG
                     VLGRPITNDLDLSLTIVLAGPTVMLVILAMVSWFLTSRFERHGARSIMIGGTILAAIG
                     VMLLALSQNLASYMTAWIVLGFAGAGVLTTAAQIAIAEIAGPRAKQAIGLLALFGALS
                     SPILWPIVNALQATIGWRSTTALLAACLITISLPLLWWALPPSPDSAPETDQMRPGAL
                     DSGPARTPLNSVDFALVAIASAANGFITWGFSLTLVVLFEERQVSRDDAVALASFIGL
                     ASLAARALDYLGGRRWSGLTTGVAASLVLPVSYLVLLAGSGYAVALTFVVLYGLAGGA
                     MAVARSTIPLAIFPRDAYARASARLALPLNLAYAA"
BASE COUNT          317 a          722 c          600 g          382 t
ORIGIN      
        1 ccaacggcgc cggcaagtcc acgacgctgc gcaccctcct cggcttcctg cgcccgacga
       61 gtggtcgcgc cgaaatcctg ggcatggatt cgcatgccga agccgtcgcg atccaccgcc
      121 ggctcgggaa tcttcccagc gagttcaatc tcgaggaccg gatgaccggc gaacagctgc
      181 tccgctactt cgccgacctg cgcggcatcg atcgcaccct cggctacgcc cacgagctcg
      241 ccgcgcggct cgacgccgat ctcacgcgcc cgatgcgacg cctctcacgc ggcaacaagc
      301 agaagatcgg gatcatccag gccctcttcc accgtcccga tgtgatcgtc ctggacgagc
      361 cgacaagcgg gctcgatccg ctcgtccagg aagaattcct cacgatcctc gaggagatcc
      421 gggcgctcgg gcagacgatc ttcttctcgt cgcacatcct gccggaaatc gagcggatcg
      481 ccgatcgcgt cggcatcatc cgcgccggtg aactggtcgc ggtcgagcag ccatcggcgc
      541 tgacggcccg cgccttccgc tacgtccgaa ttcgcttcgc ggaaccgctc tcgtcatccg
      601 ggcgtgagga gctcgccggg ctccccggtg tcgagaagtt cgccgccgat ggaagcaccg
      661 tcaccttcac cgcgcacggg gcgacggatg cgatcgtcaa gttcgccgcg acccgcgagg
      721 tcgagcagtt cgactcggaa cgcccgccgc tggaggagat cttcctcacc tactacgccg
      781 aggcgccgga cgggcggtag acaattccat aaagatgaaa tccttgctaa agttgcccca
      841 gaagcctcat caaaatgcga acatgcgatc tattcgagcc tgggccggtc gtcattcaca
      901 ggattcgcca gacgcgtgaa gagttcatct cctgaactga ccccatccgg aacagcgccg
      961 caattcgtcg tgccgctcta tccgcgtgtg gtggtgctcg gtatctccca gatcctcgcc
     1021 tggggcgcga cattcaatct ccccggcgtc ctgggaaggc cgatcaccaa cgacctcgat
     1081 ctcagcctca cgatcgtgct cgccggcccc accgtcatgc tggtgatact cgcgatggtc
     1141 tcctggttcc tgacgtcgcg cttcgaacgg cacggcgcgc ggtcgatcat gatcgggggg
     1201 acgattctgg cggcgatcgg cgtgatgctc ctcgcgctct cgcagaatct cgcgagctac
     1261 atgactgcgt ggatcgtgct cggcttcgcg ggtgccggag tcctgacaac agccgcccag
     1321 atcgcgattg ccgagatcgc cgggccgcgc gcgaagcaag cgatcgggct gctcgccctc
     1381 ttcggagcac tctcctcgcc gatcctctgg ccgatcgtga acgcgttgca agccacgatc
     1441 ggttggcgat cgacaacggc gctccttgcc gcgtgcctca tcacgatctc gctgccgctt
     1501 ctctggtggg cgcttcctcc ttccccggat tccgcaccgg aaacggacca gatgcgcccg
     1561 ggcgcgttgg attccggccc ggcacgaacc ccgctgaact cggtcgattt tgccctggtc
     1621 gccatcgcct ccgctgcgaa cgggttcatc acctggggct tctcactgac gctcgtcgtt
     1681 ctcttcgagg agcgtcaagt gagccgtgat gacgcggtcg ccctcgcttc gttcatcggg
     1741 cttgcgagcc ttgccgcccg cgccctcgat tatctcggcg gacgccgatg gagtggcctc
     1801 acgacgggcg tcgccgcttc gctcgtcctt ccagtcagct acctcgtcct cctcgccgga
     1861 tcaggatacg cagtcgccct caccttcgtc gtgctctacg gtctcgccgg cggggcgatg
     1921 gcggtcgcgc ggtcgacgat accgctcgcg atctttccgc gggacgccta tgcacgcgcc
     1981 tccgcgcggc tcgcgttacc attgaatctc gcgtatgcgg c
//
LOCUS       DASTMD010000865         1724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2137305, whole genome shotgun sequence.
ACCESSION   DASTMD010000865 DASTMD010000000
VERSION     DASTMD010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137305"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..899
                     /gene="nfo"
                     /locus_tag="VFQ54_12860"
     CDS             39..899
                     /gene="nfo"
                     /locus_tag="VFQ54_12860"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEU0165929.1"
                     /translation="MTIEFGSHVSASGGVDKVFARAEEVGATSIQIFSKNERQWTAKP
                     LEPDVVENFHRERERMGIDPKLLVIHDSYLINLASPKEENLAKSLVAFRDELERGDAL
                     GIPNLVTHPGAHTGSGVETGVARFAQSLNELFDAMPDSKTITLLETTAGQGSSLGRSF
                     EELAAIIDQVEDKSRVGVCLDTCHIFAAGYDFRTPEQYAETMGKFDEIVGIKYLRALH
                     LNDSKNPLGSFKDRHDMIGAGEIGIEGFRHFVNDARLAGIPGILETEKDPAGDNDRKN
                     LATLRDLYAG"
     gene            complement(1083..>1724)
                     /locus_tag="VFQ54_12865"
     CDS             complement(1083..>1724)
                     /locus_tag="VFQ54_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0165930.1"
                     /translation="SRIRMGAFSYAGQVCISVQRVFIHESRYDEVKTKLIAAAASLKP
                     GDPMDPSTELGPMIDEKASHRAEEWVATAEREGATILTGGHARDRFFEPTVIENARRE
                     SFVCSREAFAPLVTIFPVKSFSEGISALNDSDYGLQAGIFTNRLENALTAFERVEAGG
                     VIINDVPTYRIDHMPYGGVKSSGLSREGVRYAIEDMTEPRLMVFNRLESQRLP"
BASE COUNT          321 a          542 c          563 g          298 t
ORIGIN      
        1 gttcgtcgcg tgacttgcgg tgccgcacgg gagggatcat gacgatcgag tttggttcac
       61 acgtttccgc gtcgggcggg gtcgacaagg tgttcgcgcg ggcggaggaa gtgggggcga
      121 cctcgatcca gatcttctcg aagaacgagc gccagtggac ggcgaaaccc ctggagcccg
      181 atgtcgtcga gaatttccat cgcgagcggg agcggatggg gatcgacccg aagctgctcg
      241 tcatccacga ttcctacctg atcaaccttg cctcgccgaa ggaagagaac ctcgcgaaat
      301 cactcgtcgc atttcgcgat gagctcgagc gcggcgatgc gctgggcatc ccgaacctcg
      361 tgacccaccc cggcgcgcac accggaagcg gcgtcgagac gggcgtcgcc cgcttcgcgc
      421 agtcgctcaa cgagctcttc gacgcgatgc cggacagcaa gacgatcacg ctgctggaga
      481 cgaccgccgg ccagggatcg tcactcgggc gatcgttcga ggagctggcg gcgatcatcg
      541 accaggtcga ggacaaatcc cgcgtcggcg tctgcctgga tacctgccac atctttgccg
      601 ccggctacga cttccggacg ccggaacagt atgcggagac gatggggaag ttcgatgaga
      661 tcgtcggcat caagtacctg cgggcgttgc acctgaacga ctcgaagaac ccgctcggga
      721 gcttcaagga tcgccacgac atgatcggcg ccggggagat cgggatcgag ggattccgcc
      781 acttcgtgaa tgatgcccgt ctcgccggga ttcccggtat cctcgagacc gagaaggacc
      841 cggcgggtga caacgatcgc aagaacctgg cgaccctgcg cgacttgtac gccggatagg
      901 gccgaaagtc atccgctctt cgggggcggc ccttcgacgg gctcagggca ggtgccgaag
      961 tcgcccggcc aaggtcatat cgagctgcgc tcgatcggtg cgctgccggg ccagaccagc
     1021 tgactcgcta cggcggacgc cctggagcgg cgcttccata ggcggaagcg ccgcatcgtc
     1081 gcctatggaa gcctctggct ttcgaggcga ttgaacacca tcagccgagg ttcggtcatg
     1141 tcctcgatcg catagcgcac accctcgcga ctgagaccgg atgacttcac gccgccgtag
     1201 ggcatgtgat cgatgcggta ggtcgggacg tcgttgatga tgacgccccc ggcttcgacg
     1261 cgctcgaacg ccgtcagcgc gttttcgagc cggttcgtga agatgcctgc ctgaagccca
     1321 tagtcggagt cattgagcgc cgagattccc tcggagaagg atttcaccgg gaagatcgtc
     1381 accagcggcg cgaaggcttc acgcgagcag acgaagctct cgcgacgcgc gttctcgatc
     1441 acggtcggct cgaagaagcg atctcgcgca tgaccgccgg tgaggatcgt cgcaccctcc
     1501 cgttccgccg tcgccaccca ttcctccgcg cggtgagatg ccttctcatc gatcatcggg
     1561 ccgagctcag tcgacggatc catcggatcg cccggcttga gcgaggcggc cgcggcgatg
     1621 agcttcgtct tcacctcgtc gtagcgcgac tcgtggatga agacgcgttg gaccgagatg
     1681 cagacctgac cggcgtatga gaacgcgccc atcctgattc gcga
//
LOCUS       DASTMD010000866         4834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2224296, whole genome shotgun sequence.
ACCESSION   DASTMD010000866 DASTMD010000000
VERSION     DASTMD010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224296"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..547
                     /locus_tag="VFQ54_12870"
     CDS             83..547
                     /locus_tag="VFQ54_12870"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU0165931.1"
                     /translation="MNDHASLTTIDPDPDWMKTDCVCTSVRRVSRSLTRIYDDAMRPS
                     GLTTVQYSMLSALNRAPERVTMTQLAEAQVMDRTTLTRNLHPLEREGLVRIEAGEDKR
                     TRYVHLTETGRDRIAVARPLWQAIDREVSEEAGPGRIEALLQELADLTARIH"
     gene            590..1939
                     /locus_tag="VFQ54_12875"
     CDS             590..1939
                     /locus_tag="VFQ54_12875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165932.1"
                     /translation="MTPSPVDAISPVPARQPRFHYAWVMAAMTFLVLLGASGFRSAPS
                     ILIVPLQDAFGWSRATISLAVSINLILFGFMGPFAAASMERFGLRRVVTAALITVATG
                     SALTIFMNAPWQLYLLWGVLVGLGTGSMATVLAATVANRWFVKRRGLVMGALTAASAT
                     GQLIFLPGLAWLVANHGWKSVSIAVAATTLAVIPLVLIFGRNRPADIGLRPYGATETD
                     LPVPPRVNPIGAAFASLRIAVTSRDFWLLAGSFFICGATTNGLIGTHLIPAGVDHGLS
                     EVAAANLLAFIGIFDIIGTTVSGWLTDRYDARRLLFAYYGFRGLSLMVLPWAFGSPHA
                     GLILFIVFYGLDWVATVPPTVALTAKSFGVGQAPLIFGWIFAAHQVGASVAAYVAGVV
                     RTEFGDYFLAFTSAGWLCFVAAALVLRIGRPSRRTLRDRRRDASPVSSPALGTTGSD"
     gene            2511..3368
                     /locus_tag="VFQ54_12880"
     CDS             2511..3368
                     /locus_tag="VFQ54_12880"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0165933.1"
                     /translation="MTHPITPFLWFDTNAEEATAFYTSIFQNTEAHSGTSFRLDGQEF
                     LSLNGGPHYHFTPAISFYVLCETETEIDELWARLNEGGTALMELAQYPFSKKFGWVQD
                     RFGLSWQLSLAEVPQTVNPFLMFVGDQYGRAEEAINFYLTVFPESRINHIERYPASGS
                     ASGSAGGTEKEGGVMFSSFTLNGTDFMASENGYDHNFTFSEATSFFVSVQTQEEVDTY
                     WEKLTAGGAPGQCGWLKDRFGVSWQVIPEILGQYLQDADREKANRVIQAMLRMTKIDI
                     AGLEAAYSA"
     gene            3488..4759
                     /gene="fabF"
                     /locus_tag="VFQ54_12885"
     CDS             3488..4759
                     /gene="fabF"
                     /locus_tag="VFQ54_12885"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU0165934.1"
                     /translation="MTDQLSPTTPARRVVITGTGAICSVGNTAPEVWAALKAGTSGIA
                     PITRFDPTIFETRFAGEVKGFDPAEQLGRKEHRRLDRYSQLAIVAAREAVVQSGLEIA
                     PEATRVGVLMATAMGGMETFETGTELMLTQGPRRVSPFFVPMMLANMAAGTVAIDLGT
                     KGPNFATVSACASAGHAIGEGVQMIRDGRADAIVAGGAEAPVTRMGITGYNAMDALST
                     RNDDPAAASRPFDSNRDGFVLAEGAAAIVIEEREHAIARGATILGEVIGYATTDDANH
                     IVQPGPEGEGIARAMELALTDAGIDRQQIGYINAHGTSTQMNEKFETMGYKRVFGDVA
                     YQIPISSTKSMTGHLLGAAGSLEAVISTHALLEGILPPTINQTDPDPDCDLDYIPNVA
                     RQSDAKVAMSNSMGFGGHNVSLIFAHPDFAR"
BASE COUNT          819 a         1693 c         1492 g          830 t
ORIGIN      
        1 cgccagggga ctactacgat ctaatccgtg catatacaca gataccaatc cgtccgcctt
       61 tccgcatcga gcgaccgcac ctgtgaacga tcacgcatcc ctgaccacta tcgaccccga
      121 tccggactgg atgaagaccg attgcgtctg cacctcggtt cgtcgcgtca gccgctcgct
      181 gacccggatc tacgacgacg cgatgcgccc gagtgggctc acgaccgtcc agtactcgat
      241 gctctccgcc ctcaaccggg cgccggagcg ggtgacgatg acccagctcg ccgaggccca
      301 ggtgatggac cggaccacgc tcacccgaaa cctgcatcca ctcgagcgcg agggactggt
      361 ccggatcgag gcgggagagg acaagcgaac gcgctacgtc catctcaccg agaccggcag
      421 agaccgcatc gccgtcgcgc gtcccctctg gcaggccatc gatcgggagg tctcggagga
      481 agccggaccg ggccggatcg aagccctcct ccaggaactc gccgatctca ccgcacgcat
      541 ccattgacct tcggaccatc caccccgcca catgacaggg acatcctcga tgacgccatc
      601 tccagtcgat gcgatctcac ccgtgccggc ccggcagccc cgcttccact acgcgtgggt
      661 gatggccgcg atgaccttcc tcgtgctgct tggcgcctcg ggattccgct ccgccccgag
      721 catcctgatc gtgccgctgc aggatgcctt cggctggagc cgggcgacga tctcgctcgc
      781 cgtctcgatc aacctgatcc tcttcggctt catgggtccc tttgccgccg cctcgatgga
      841 acgcttcggc ctccggaggg tcgtcaccgc ggcactgatt accgtcgcga ccggctcggc
      901 actgacgatc ttcatgaacg cgccctggca gctctacctg ctctggggcg tcctcgtcgg
      961 actgggaacc gggtcgatgg cgaccgtcct cgcggccacc gtcgccaacc gctggttcgt
     1021 caagcggcgc ggtctggtga tgggcgcgct gaccgcagcg agcgcgaccg gccagttgat
     1081 cttcctcccc ggtctcgcct ggctcgtcgc gaaccacggc tggaaatcgg tctcgatcgc
     1141 ggtcgccgcc acgacgctcg ccgtcatccc gctcgtcctg atcttcggac gcaaccgtcc
     1201 cgccgacatc gggttgcggc cctacggggc gacggagact gacctcccgg tgccgccgcg
     1261 cgtgaatccg atcggcgccg ccttcgcgag tctgcggatc gcggtgacgt cgcgggattt
     1321 ctggctcctg gcggggagct tcttcatctg tggcgcgacg acgaacgggc tgatcggcac
     1381 ccacctgatc ccggctggag tcgatcacgg cctttccgaa gtggcggcgg cgaacctgct
     1441 cgccttcatc ggcatcttcg acatcatcgg tacgaccgtc tccggctggc tgaccgatcg
     1501 ctatgacgcg cgacggctcc tcttcgccta ctacggcttc cgcgggctct cgctgatggt
     1561 gctgccgtgg gcattcggat cgccgcatgc cgggctgatc ctcttcatcg tcttctacgg
     1621 gctcgactgg gtggcgaccg tgccgccgac cgtcgccctc accgcgaagt cgttcggggt
     1681 tgggcaggcg ccgctcatct tcggctggat cttcgcggcg catcaggtgg gagcgtcggt
     1741 ggcggcgtac gtcgccgggg tcgtccgcac cgaattcggc gactacttcc tcgccttcac
     1801 gagcgcaggc tggctctgct tcgtcgccgc cgcgctcgtg ctgaggatcg gtcgcccctc
     1861 ccgccggacg ctgcgtgacc ggaggcgcga tgcgtctccg gtttcgagcc cggccctcgg
     1921 cacgaccggc tcggactgac gcgatccccg cgccggattc caccctacga acgcacgcgg
     1981 tcgatacgat cgtcccactg acgtggagcg ttaagactgg attgtctgac gatttcccgt
     2041 tgacgtggcg cccattcagg tggtcgttgg cggtcccaac gcccccgtca tcctgagcgg
     2101 agcgaaggac ccccgcgcga agcgcacccg ccaccggcgg gctccaacac gatcgtattg
     2161 gggcggcgtt ccccggagac ggatcggctc cgccgacccg gaggcggcca cgggggccgc
     2221 cgggacggga ttcccgaagg tgtgttggac acgtgaaccc tgacactcca ttcagcggtg
     2281 catccgcgaa gtccttccat ccctctcagc aagatatcga gctacgcgcg atgggttaca
     2341 cggggtgatt cgctacgacg ggacctccgt gtcccgcctc tactgcatgc cggaggcgca
     2401 aggaggaaga agcatccgcg aggttcctct tctatgctgg aagccaggat gaattgacaa
     2461 tctcgaagtc cgcgtcgacg ctgactgtcg gcagaatgag gagtatcgcg atgacgcacc
     2521 cgatcacccc gtttctctgg ttcgacacca acgccgagga ggcgaccgcc ttctacacct
     2581 ccatcttcca gaacaccgag gcccacagcg gcacatcctt ccgccttgat gggcaggaat
     2641 tcctctcgct caacggcggg ccgcactacc acttcacgcc cgcaatttcc ttctacgtac
     2701 tctgtgagac cgagacggag atcgacgagc tctgggccag gctcaacgag ggtggcacgg
     2761 ccctgatgga gctcgcgcaa tatcccttca gcaagaaatt cggatgggtg caggaccggt
     2821 tcggactctc ctggcaactc agcctcgcgg aggttccgca gacggtcaat cccttcctta
     2881 tgttcgtcgg cgaccagtac ggccgcgccg aggaagccat caatttctac ctcaccgtct
     2941 tccccgaatc gcgaatcaat cacatcgaac gctatcccgc cagcgggtcg gccagcgggt
     3001 cggccggcgg cacggagaag gaaggcggcg tgatgttctc ctcgttcacg ctgaacggga
     3061 ccgatttcat ggcgtcggag aacgggtacg atcacaactt caccttcagc gaggcaacat
     3121 cgttcttcgt cagcgtgcag acgcaggaag aggtcgatac ctactgggag aagctgaccg
     3181 ccggcggcgc gcccggtcaa tgcggctggc tgaaggacag gttcggcgtc tcctggcagg
     3241 tcatcccgga gatcctgggc cagtacttgc aggatgcaga tcgcgagaag gcgaaccgcg
     3301 tgatccaggc gatgctgcgg atgacgaaga tcgatatcgc cgggctcgaa gccgcataca
     3361 gcgcgtagtt ccgtacgtca ccctcgatcc cgcgccccgc gaggcgccgc ggaagttaag
     3421 attggcgaca tcagccccgg tcgatgctga ccggggtcgt tatgttctcc tcaagactgg
     3481 atacaccgtg accgatcagc tctctcccac cacgcccgct cgccgcgtcg tcatcaccgg
     3541 caccggcgcc atctgctctg ttggcaacac cgcgcccgag gtctgggccg cgctcaaggc
     3601 gggaacgtcc ggcatcgcgc cgatcacccg cttcgatccc acgattttcg agacccgctt
     3661 cgccggcgag gtgaagggat tcgatcctgc cgagcagctc ggtcgcaagg aacatcgccg
     3721 cctcgatcgc tactcgcagc tggcgatcgt cgccgcccgc gaggcagtcg tccagtccgg
     3781 actcgagatc gcccccgagg cgacccgcgt cggggtgctg atggcgacgg ctatgggcgg
     3841 catggagacc ttcgagaccg gcaccgaact catgctgacc cagggcccgc ggcgcgtttc
     3901 ccccttcttc gtaccgatga tgctcgcgaa catggcggcg ggaacggtgg cgatcgatct
     3961 cggcaccaag ggcccgaact tcgcgaccgt ctccgcctgc gcgagcgccg gccacgcgat
     4021 cggcgagggc gtccagatga tccgcgacgg gcgggccgat gcgatcgtcg ccggcggcgc
     4081 cgaggccccg gtgacccgga tggggatcac cggctacaac gcgatggatg ccctctcgac
     4141 ccgcaacgac gatcccgccg ccgcgagccg gccattcgac agcaaccgcg acggcttcgt
     4201 cctcgccgag ggcgcggccg cgatcgtgat cgaggaacgc gagcacgcga tcgcccgcgg
     4261 tgcgacgatc ctcggtgagg tcatcggcta cgcgaccacg gatgacgcca accacatcgt
     4321 gcagccgggc ccggagggcg aagggatcgc ccgcgcgatg gagctcgccc tcaccgacgc
     4381 cgggatcgat cgccagcaga tcggctacat caatgctcac ggcacctcga cccagatgaa
     4441 cgagaagttc gagacgatgg gctacaagcg ggtcttcggt gacgttgcct accagatccc
     4501 gatcagctcg acgaagtcga tgaccggtca cctcctcggc gcggcgggct cgctggaagc
     4561 ggtgatctcc acccacgccc tgctcgaggg catcctgccg ccaacgatca accagaccga
     4621 tcccgatccc gactgcgatc tcgactacat cccgaacgtc gcccgccaga gcgacgcgaa
     4681 ggttgcgatg tccaactcga tgggattcgg cggccacaac gtctcgctga tcttcgccca
     4741 cccggacttc gcaaggtagg aagcgggaca gagaagtccc gccctacgaa cgaccgattt
     4801 ccgcgtcgtg gcgggtcacc ccgtgtgacc catc
//
LOCUS       DASTMD010000867         1637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_618216, whole genome shotgun sequence.
ACCESSION   DASTMD010000867 DASTMD010000000
VERSION     DASTMD010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618216"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1074)
                     /locus_tag="VFQ54_12890"
     CDS             complement(247..1074)
                     /locus_tag="VFQ54_12890"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEU0165935.1"
                     /translation="MQNDVMPDGTASTVSAATPDDKYKIASVQRAMALLGAFLEPPHQ
                     FGVSELSRMLGQTKNQTFRLLQTLADEGAVVIDTETKKYSLGYRMLELGIMAQKGSPL
                     TIALSPIMDQLALESGETVVLTRMADDMSAICVDKRESSQALQISARIGSRISLHAGA
                     GSKALLAFSSPEFIERFLARAQPLRRYLAGTITDPDELRAELAHIRERGYSVSDNDLD
                     EGAASVSAPIRNHRGEVVAAISVASPRTRFTPDDFARNRQLVLNAAINATNRLQGYR"
     gene            1389..>1637
                     /locus_tag="VFQ54_12895"
     CDS             1389..>1637
                     /locus_tag="VFQ54_12895"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEU0165936.1"
                     /translation="MGATVIVDAFWGDSGKGKVSAWLARKLGVTLGVRAGIGTNAGAS
                     VILPDDSVIKQRQLPTTWMNGTSRVAVGSGVLVDPTVFL"
BASE COUNT          284 a          477 c          525 g          351 t
ORIGIN      
        1 aaatcgcggc gggaagtggt gatcccggct cgctgcagtt cttcctggaa tgcctcgatc
       61 ttcatgagca acggtctccc aaggcccgag atctgcgatc tcgtccgagt caagcaacag
      121 gttgatttga gtccccgtgg cgtcgttgcc aggtaccggc ggcgtggtgc cggtacgcta
      181 tcgatttcgg accactacgc tcctggatct gctcggtggg ccgtcgggat gatcatccga
      241 tcagcctcac cggtatccct ggagccgatt cgtggcgttg atggcagcgt tcaggacgag
      301 ttggcgattg cgcgcgaagt cgtccggcgt aaagcgcgtc cttggtgagg cgacactgat
      361 cgcggcgacg acctctccgc ggtgatttcg gatgggggcg gatacggatg ctgccccttc
      421 atcgagatcg ttgtcgctga ccgaatagcc tcgttcgcga atatgcgcga gttcggcgcg
      481 gagttcgtca ggatccgtaa tggttccagc caggtaccgt cgtagcggct gggcacgcgc
      541 caggaagcgc tcgatgaatt caggtgagct gaacgccaga agcgccttgg aaccggctcc
      601 ggcatgcagt gagatgcggc tcccgatcct ggcgctgatc tggagcgcct gcgaactctc
      661 tcgcttgtcg acacagatcg ccgacatgtc gtccgccatc ctggtcagca cgaccgtctc
      721 gccgctctcc agtgccaact gatccatgat gggcgagagc gcgatggtga gcggcgaccc
      781 cttctgcgcc atgatcccca actccagcat ccggtacccg aggctgtatt tcttcgtttc
      841 ggtatcgatt actacagcac cctcgtcggc gagcgtctgc aggagtcgaa aggtctgatt
      901 cttggtctga ccgagcatgc ggctaagctc gctgacgccg aactggtgtg ggggttccag
      961 gaaggcaccg aggagagcca tcgctcgttg cacgcttgcg atcttgtact tgtcgtccgg
     1021 cgtcgctgcc gaaactgttg aggccgttcc atcaggcata acgtcgttct gcacaataaa
     1081 catcctagtg tgttcgggag aggttggtca attccctctc ggggcaatgg aggtcgtggg
     1141 ggaatggcgt ctgtatccac gtcacaaccc cgacccggtc aagtctgtac aacttgcgga
     1201 tggcggcgat ctcccctgca tggatcagag attatgcaat tcttctggag aaccggacat
     1261 caccgcccga tcgcgggtga ccgccggcgg acgattgccg cgatgcgtgc gaaaatgatc
     1321 gtcgtttgcc acgcgacccg gttgttcgcg gtggtgttgt gtcaagcttg ccacgggggc
     1381 cagtcgtcat gggtgccacg gtcatcgtcg atgcgttctg gggagattcc gggaaaggga
     1441 aggtcagtgc ctggctcgcg cgcaagctcg gggtgacgct gggtgtccgc gccggaatcg
     1501 gaaccaacgc cggggcgagc gtgattctcc ccgatgattc cgtgatcaag cagcgccagc
     1561 tgccgacgac ctggatgaat ggcacgtcgc gagtcgcggt cggatccggc gttctcgtcg
     1621 atccgacagt cttcctc
//
LOCUS       DASTMD010000868         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1511692, whole genome shotgun sequence.
ACCESSION   DASTMD010000868 DASTMD010000000
VERSION     DASTMD010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511692"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFQ54_12900"
     CDS             <1..469
                     /locus_tag="VFQ54_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU0165937.1"
                     /translation="LGAEQIILLGRHPVRIALAEEFGATDIVTERGEEAIERVRELTG
                     GFGAHSVLECVGLGESTRTAIGIARPGGAVGRVGVPQEASIPEAASTLFRNVSIGGGI
                     APVRAYIEELLPDVLEGRIQPGRVLDRAVSLDGVPDGYRAMNDREAIKVMIAF"
     gene            498..875
                     /locus_tag="VFQ54_12905"
     CDS             498..875
                     /locus_tag="VFQ54_12905"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HEU0165938.1"
                     /translation="MTAWNNDELTTIGTAEELRIASRRRDGTLRNPVTIWVVRHGDDL
                     YVRSVNGRDAAWFRGTRVSHEGQIRAGGITREVTFVDSDGSLDDQIDAAFRTKYRRYA
                     ARVIESIVSPNARAATLKLVPRE"
     gene            1042..1497
                     /locus_tag="VFQ54_12910"
     CDS             1042..1497
                     /locus_tag="VFQ54_12910"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0165939.1"
                     /translation="MSRLEAVTAITQENDMQITRNSIETRRGPGEWFTGAVYVDTVTA
                     PTGESRLSASSVHFTPGARTAWHTHPIGQTIYVTEGIGLTQRRGGPIEIIRPGDRVFF
                     EPGEDHWHGAAPDRFMSHLAMLEVDAEGTSAVWGDHVTDDEYGYELPAE"
     gene            1628..>2008
                     /locus_tag="VFQ54_12915"
     CDS             1628..>2008
                     /locus_tag="VFQ54_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEU0165940.1"
                     /translation="MPITPGKWIATSSEYRPDRYYRYAELTELLQRWVAEHPDLLAIE
                     RIGTSFEGRDIFALTLTDFSTGDPAEKPAYFVDANIHADEVTGVATVLWLIDHVLSNA
                     TTDDAIARLLSETTLYLVPAVNVDA"
BASE COUNT          371 a          685 c          634 g          318 t
ORIGIN      
        1 tctcggcgcc gagcagatca ttctcctcgg gcgtcatccg gtgcgcattg cgctggcgga
       61 ggagttcggc gcgaccgaca tcgtgaccga acgcggagag gaagcgatcg agcgggtgcg
      121 cgaactcacc ggcggcttcg gcgcccactc cgtcctggaa tgtgtcggcc tcggggagtc
      181 gacacgcacg gcgatcggca tcgcccggcc gggcggcgcg gtcgggcgcg tcggggttcc
      241 ccaggaagca tcgatcccgg aggcagcctc gacgctcttc cgaaatgtct cgattggcgg
      301 cggcatcgcc ccggtccgcg cctacatcga ggaattgctc ccggacgtgc tcgagggtcg
      361 gatccagccc ggtcgggtcc tcgatcgcgc tgtcagcctc gacggggtac cagacggcta
      421 ccgggcgatg aacgaccgcg aagcgatcaa ggtcatgatc gccttctgat cgacctatac
      481 agaaacggga tccgccgatg acggcatgga acaacgacga actcaccacg atcggaacag
      541 ccgaagaact gcggatcgcg tcacgacgac gtgacggcac gctccgcaac ccggtcacca
      601 tctgggtggt ccgccacggg gacgacctct acgtccgctc ggtcaacgga cgcgatgccg
      661 cctggttccg tgggacccgg gtgagccacg agggtcagat ccgggcgggc ggcatcacca
      721 gggaagtcac cttcgtggac tccgacggca gccttgacga ccagatcgac gccgcgttcc
      781 gcaccaagta tcgccgttac gcggcacgcg tcatcgagag catcgtcagc ccgaatgcac
      841 gcgccgcgac actcaagctc gtgccccgcg agtaggagac ccggacagcg gggcattgcc
      901 gtccccgatc ttccggtgta tccatcacat gctgaacccg atcgcgtcgc ttctgaaggg
      961 ggactccatt cgacgaactc ggcgccatcc gaagtctccg accacggtgc gtacagcgtt
     1021 cgttcgcagg ataggacttc catgtcccgc ctcgaagctg tcacggctat cacgcaggag
     1081 aacgacatgc agatcacaag aaactcgatc gaaacccggc gtgggccggg cgagtggttc
     1141 accggcgcgg tgtatgtcga caccgtgacc gccccgaccg gtgaatcgcg gctgagcgcg
     1201 agcagcgttc acttcacccc gggcgcccgt accgcctggc acacgcatcc gatcggccag
     1261 acgatctacg tcaccgaggg catcggtctc acccagcggc gcggcggccc gatcgagatc
     1321 atccgcccgg gtgaccgcgt cttcttcgaa ccgggagaag atcactggca cggcgccgca
     1381 ccggaccgct tcatgtccca tctcgcgatg ctcgaggtcg atgccgaagg cacgagcgcc
     1441 gtctggggcg atcacgtcac cgacgacgag tacggctacg aactgccggc ggagtaagtg
     1501 ctgccaatcc gccctcccgc tcgtagaatg cgcctacgca tccgcaccag cgcccgtccg
     1561 gggcaatgaa tccgggcagt gcgccgatcg atcgctccgt cgacgtcttc acgggaggaa
     1621 ccagggcatg ccgattacgc cggggaagtg gatcgccacg tcgagcgagt accgtccgga
     1681 ccgctactat cgatacgcgg aactcacgga gctgctccag cgctgggtgg cggaacatcc
     1741 ggacctgctc gcaatcgagc gcatcggcac ctcgttcgag gggcgcgaca tcttcgcgct
     1801 gactctgacg gacttctcga ccggcgatcc ggcggagaaa cccgcctatt tcgtcgatgc
     1861 caacatccat gcggatgagg tgaccggtgt cgccaccgtg ctctggctca tcgatcatgt
     1921 cctctcgaat gccactaccg acgacgcgat cgcgcgactc ctcagcgaga ccacgctcta
     1981 cctcgtgccg gcggtcaatg tcgacgcg
//
LOCUS       DASTMD010000869         1590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_259136, whole genome shotgun sequence.
ACCESSION   DASTMD010000869 DASTMD010000000
VERSION     DASTMD010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259136"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFQ54_12920"
     CDS             <1..367
                     /locus_tag="VFQ54_12920"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0165941.1"
                     /translation="TEIPVSAAAGAGALVSTVADLDRWLQALLDGFAIGEELRNERMN
                     LIPTGEEHFGYGLGVMSHGRLVGHGGGIPGYTSFAGRDPETGVNIVVLTNGEGNGTPE
                     GSVMALVDTIVKLLPTAAE"
     gene            complement(375..1418)
                     /locus_tag="VFQ54_12925"
     CDS             complement(375..1418)
                     /locus_tag="VFQ54_12925"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU0165942.1"
                     /translation="MSQNPTRARDLGIVPGRFEPGPLNAMTDVPGVRVGQTTLIDAPD
                     IHTGVTAIVPDQLMALRTLPANLFVGNGYGKLIGSTQLVELGEIETPILLTGTLSAFR
                     VADALVSYMLSLPGNEEMTSVNPIVGETNDGFLSDIRSRPISEAHVLAAISAAATGPV
                     AEGCVGAGTGTTALGFKAGIGTSSRVVEIEGRDALATIGVLVQANFGGTLQVTGTSIP
                     ADEAIRWSGERHETNEAEQGNSCMIVVATDAPLDARQLGRVATRAVFGMARVGADYTH
                     GSGDYGIAFSVGEGPVLRDAELNPIFIATQEAVEEALLNSLFMATTTIGYRKRVRHAV
                     PHAFVLDQLARRA"
BASE COUNT          302 a          526 c          486 g          276 t
ORIGIN      
        1 gaccgagatt cctgtctccg cggcggccgg agccggcgca ctggtctcga ccgttgccga
       61 cctcgaccgt tggttgcagg cactcctgga cggcttcgcg atcggcgagg agcttaggaa
      121 tgagcgcatg aatctcatac cgaccggtga agagcacttc ggatacggac tcggcgtgat
      181 gagccacggc cgcctggtcg gccacggcgg cggcatcccg ggctacacga gcttcgccgg
      241 tcgcgatccc gagaccggtg tcaacattgt tgtccttacg aacggcgagg gcaacggcac
      301 gccagaagga tcggtcatgg cgctggtcga cacgatcgtc aagctccttc caactgcagc
      361 ggagtagtcg ttcctcacgc gcgccgcgcc aactgatcca ggacaaacgc gtgcggcacg
      421 gcgtgtcgca cgcgtttgcg gtacccaatc gtcgtcgtcg ccatgaagag cgaattgagg
      481 agcgcctcct cgaccgcttc ctgcgtggcg atgaagatcg gattcagctc cgcgtcgcga
      541 agcacgggcc cctccccgac cgagaacgca attccgtaat caccacttcc atgggtgtag
      601 tccgccccga cgcgcgccat cccgaagacg gcccgggtcg ccacccgacc cagttgccgg
      661 gcgtcgagcg gcgcgtcggt cgcaacaacg atcatgcagg agtttccctg ctcggcctcg
      721 ttcgtttcgt gacgttcccc agaccagcgg atcgcctcat ccgcgggaat cgacgtgccc
      781 gttacctgca gcgtgccgcc aaaattcgcc tgaacgagca ccccgatcgt cgccaaggca
      841 tcgcgtccct cgatctcgac gacccgcgac gatgtaccga tccccgcctt gaagccaagc
      901 gccgtcgtgc ccgtcccggc accgacgcat ccctcggcca ccggccctgt cgcggcggcg
      961 gaaatcgccg ccaggacgtg cgcttccgaa atcgggcgtg agcggatatc ggagaggaaa
     1021 ccgtcgttcg tctcaccgac gatgggattg acggaggtca tctcctcgtt gccgggaagc
     1081 gaaagcatgt agctgacgag ggcgtctgcg acgcggaagg cggaaagcgt cccggtgagc
     1141 aggatcggcg tctcgatctc acccaactcg acgagttgcg tcgatccgat cagcttgccg
     1201 tagccattgc caacgaagag atttgccggg agtgttcgca gggccatcaa ctggtccggg
     1261 acgatcgccg tgacaccggt gtggatatcc ggggcgtcga tcagcgtcgt ctgtccgacg
     1321 cgcacgccgg ggacatcggt catcgcgttg agcggcccgg gctcgaaccg acccggaacg
     1381 atgccgagat cgcgtgctcg cgttgggttc tgactcatga tcatcctctc tccagcacga
     1441 gacacgtatc ggggtttccg gatgtaattc catccgatat actggatgaa ttagcacgct
     1501 ccgcgaaagg aaccagcatc atggctcgcg atcccctcaa ggaagaacca cctgtcagaa
     1561 ccatcagttc gaacgacgcc aaacagcact
//
LOCUS       DASTMD010000870         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1599328, whole genome shotgun sequence.
ACCESSION   DASTMD010000870 DASTMD010000000
VERSION     DASTMD010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599328"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..551
                     /locus_tag="VFQ54_12930"
     CDS             168..551
                     /locus_tag="VFQ54_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0165943.1"
                     /translation="MTMTNLDTQAIGCPVARTAEIIGNKWTPLIVRDLATGQKRFSEL
                     ERSLIGISPKTLSERLKRLEEAAVVNRKCYAEVPPRVEYTLTEKGYALLPVIDSMRAF
                     GNTWLSPEGCDDDVPSTVAELAAAI"
     gene            complement(555..>1509)
                     /locus_tag="VFQ54_12935"
     CDS             complement(555..>1509)
                     /locus_tag="VFQ54_12935"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEU0165944.1"
                     /translation="AVLTMERVDGIRIDNVDQIREAGFDPIAIAQYTMQSLAQQILEV
                     GMFHADPHPGNFVVCADGAIGIYDFGLVGSLDERIRERMLFLAMAVTERDPVRVVDEI
                     ASLGAVPGEGDRRALERDIGHLLTQYVGVTLREMPLVTILDDAMTMIRRYRLRLPAEL
                     ALLVKTATMAEALARRLDPDINAVEVGEPMIRAAVRRFYTPSFWRDRFRGRPLEVALL
                     SAALPGHIQRFISRLDRNDFTFNMQVNDLPTTLHEMNSMVNRLVLAIIAAAGAIGTAM
                     LVLAVKPVWGSWQGYALLLIFIAVTLLIINVLFRVWRAGRR"
BASE COUNT          318 a          503 c          437 g          251 t
ORIGIN      
        1 ttcgagaaag agacgtccgg acaaggcatc cgcaagacag tcgcagtata gcgggaagac
       61 ggcccgatcg gcccatgatt gtcgcaccac cccagtactg ctatcataac gaaagtattg
      121 gacaggacgc gtctatcggt tgcctccctc ggcagcatct ggcaggcatg actatgacga
      181 acctcgacac gcaggctatt ggttgtccgg tcgcgcggac tgcagagatt attgggaaca
      241 agtggacgcc gctgatcgtt cgcgatctcg ctaccggtca gaagcggttc agcgagctcg
      301 aacgctccct gatcgggatc agcccaaaga cactctcgga gcggctgaag cgcctcgagg
      361 aggcagctgt cgtcaaccgg aagtgctacg ccgaagtgcc accgcgcgtc gagtacacgc
      421 tgacggaaaa gggatatgcg ctcctgccgg tcatcgacag catgcgcgct ttcggcaaca
      481 cctggctctc gcctgaaggt tgcgacgacg acgtaccatc cacggtcgcc gaactcgccg
      541 ccgcgatcta gagactatcg cctccctgct cgccagacgc ggaagaggac gttgatgatc
      601 aacagcgtca ccgcgatgaa gatcagcagc agggcatagc cctgccaact cccccacacc
      661 ggcttcaccg cgagcacgag catcgcggtc ccgatcgccc ccgccgccgc aatgatcgcc
      721 agcaccagac gattcaccat gctgttcatc tcgtgcaacg tggtcggcag atcgttcacc
      781 tgcatgttga acgtgaagtc gttccgatcc agccgtgaga tgaatcgctg gatgtgaccg
      841 ggtagcgccg cgctcagcaa cgccacctcg agcggtctcc cacggaaccg gtcgcgccag
      901 aacgacggcg tatagaagcg gcgcacggcg gcccgaatca tcggctcacc cacttcgacc
      961 gcattgatgt cgggatcgag ccgcctcgca agcgcctccg ccatcgtcgc cgtcttcacc
     1021 agcaacgcca gttctgccgg caaacgcaat cgataccggc ggatcatcgt catcgcgtcg
     1081 tcgagaatcg tcacgagtgg catctcccgc agggtgacgc cgacgtattg cgtcagcagg
     1141 tgcccgatat cgcgctcgag cgcgcggcga tccccctcgc cgggaaccgc accgagtgac
     1201 gcgatctcat cgacgacccg gacgggatcg cgctccgtga ccgccatcgc aaggaagagc
     1261 atccgctcgc gaatccgctc gtccagggat ccgaccaacc cgaagtcgta gatgccaatc
     1321 gccccgtccg cgcagacgac gaaattgccg ggatgcggat cggcgtgaaa catacccact
     1381 tcaagaatct gctgggcaag agactgcatc gtgtactgcg cgatcgcgat cggatcgaag
     1441 cccgcctccc ggatctggtc gacgttgtcg atccggattc cgtcgacacg ctccatcgtc
     1501 agcacggcg
//
LOCUS       DASTMD010000871         1945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_973620, whole genome shotgun sequence.
ACCESSION   DASTMD010000871 DASTMD010000000
VERSION     DASTMD010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973620"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VFQ54_12940"
     CDS             complement(<1..1115)
                     /locus_tag="VFQ54_12940"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165945.1"
                     /translation="MTKAGAFTFVLHSHLPYARQAGMWPHGEEWVHEAIAETYLPLWD
                     ALYDLADDGVDFRITVSVTPILAEQLADPLIHEHFITYAEERAAWAAQDIDRFGASGD
                     RQMQKVARFYHHWYSRRLANFRDRYNRDLLGALKVLQDRGYVEISTSAATHGYLPLLS
                     RDSSIYGQIQTGVTSFERHFGRKPKAIWLPECAYRPAYQETSPDGTTVRRPGIESFLA
                     AQGIQVFFSETHTVEGGQPIGKAAGEAIGPYENISRRYVIEHDAEEQREPGTTFRAYW
                     VGDAPGEVAVLGRNNRTGQQVWSGDFGYPGDALYREFHRKDGQSGMQYWRIGGPSVDL
                     GDKPPYDPERAAEQVKTHAQHFVGLVEQLLVNYHETSG"
     gene            complement(1213..>1945)
                     /locus_tag="VFQ54_12945"
     CDS             complement(1213..>1945)
                     /locus_tag="VFQ54_12945"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU0165946.1"
                     /translation="VTGLLRMVDIIRIDHFRGFGAAWVVPADAENAAAGHWELAPGGE
                     VFAAINRDLGDVPVIIEDLGVITPDVIALREVLGLPGMNVLQFAFDGDPENVYLPHNY
                     RRDSVVYTATHDNQTTIGWFQSRSDREQDLVRHYTGTSGEDIAWDMIRLALASVANTA
                     ILSLQDILRLDDAARMNVPGQAIGNWSWRYQDWQLEDGLADGLRSLTHTYGRAATDGA
                     PRDPNPWDYTVRRPNVTLIDPFAAP"
BASE COUNT          354 a          606 c          628 g          357 t
ORIGIN      
        1 ccactcgtct cgtgataatt gaccagcaac tgctcgacaa gaccgacgaa atgctgggcg
       61 tgggtcttaa cctgttcggc ggcacgctcc ggatcgtatg gcggcttgtc gccgagatcg
      121 acgctcgggc cgccgatacg ccagtactgc atgcccgact ggccatcctt cctgtggaat
      181 tcgcgataga gcgcgtcccc gggatagcca aagtcgccgg accagacctg ctgccccgtg
      241 cggttgttcc ggccaaggac agccacctct cccggtgcat ctccgaccca ataggcgcga
      301 aacgtcgtcc ccggctcgcg ctgttcctcg gcatcgtgct cgatgacgta gcgccgggag
      361 atgttctcgt acgggccgat cgcctctccc gccgccttgc cgatcggctg cccaccctcg
      421 acggtgtgcg tctcgctgaa gaagacctgg atcccctgcg cagcgaggaa tgactcgatc
      481 cctgggcgcc gcaccgtcgt gccgtccggc gatgtctcct gatacgccgg gcgatacgcg
      541 cattccggca gccagatcgc ctttggcttg cggccgaagt ggcgttcgaa ggaagtcacc
      601 ccggtctgga tctgtccgta gatcgaggag tcgcgcgaga gtagcggcag gtagccgtgc
      661 gtcgcggcgc tcgtggagat ctcgacgtag ccccgatcct ggaggacctt gagggcgcca
      721 aggaggtcgc ggttgtagcg atcgcggaag ttcgcgagcc ggcgcgaata ccagtgatgg
      781 tagaagcgcg cgaccttctg catctggcgg tcaccggaag cgccgaagcg atcgatgtcc
      841 tgcgccgccc aggccgctcg ttcctcggcg taggtgatga agtgctcgtg aatgagcggg
      901 tcggcaagtt gctcggcgag aatcggcgtc acgctgaccg tgattctgaa gtcgacacca
      961 tcgtcagcga gatcgtacag ggcgtcccag agcggcaggt aggtctcggc gatcgcctcg
     1021 tggacccact cctcaccgtg gggccacatg cccgcctggc gtgcgtaggg caggtggctg
     1081 tgcaagacga aggtgaatgc gcccgctttg gtcatgaagg tctcctcgcg aggtcgttcc
     1141 cgcgccggtt gaggtcgcgc gaaccggaat ccgtttcgtc agtacattgc acggttgctg
     1201 ccacatacga attcatggag ccgcgaaggg gtcgatcaac gtgacattcg ggcgacgaac
     1261 ggtgtaatcc cacggattgg gatcgcgcgg cgccccgtcc gtggcagcgc gtccataagt
     1321 atgtgtgagc gaccgcagtc cgtcggcaag gccgtcctcg agctgccagt cctgatatcg
     1381 ccaggaccag tttccgatcg cctgccccgg cacattcatc ctcgcagcat cgtcaaggcg
     1441 aaggatgtcc tgaagcgaaa ggatcgcggt gttcgccact gaggcgagtg cgaggcgaat
     1501 catgtcccag gcgatatcct ctccggaggt tcccgtgtaa tgacggacga gatcctgctc
     1561 gcggtccgac cgggactgga accatcctat cgtcgtctga ttatcgtggg tcgcggtgta
     1621 gacgacggag tcccgccggt aattgtgagg gaggtagacg ttctccggat cgccgtcgaa
     1681 ggcgaactgg agcacattca tgccgggaag tccgagaacc tcacgaagcg cgatgacgtc
     1741 gggagtgatg acgccgagat cctcgatgat gacgggcaca tccccgagat cgcgatttat
     1801 cgccgcaaag acctcgccac ccggagcgag ttcccaatga cccgcagccg cgttctcggc
     1861 gtcggcaggc acgacccagg cagcgccgaa tccccggaag tgatcgatcc ggatgatatc
     1921 gaccatccgg aggagaccag tcacc
//
LOCUS       DASTMD010000872         1996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2939532, whole genome shotgun sequence.
ACCESSION   DASTMD010000872 DASTMD010000000
VERSION     DASTMD010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2939532"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFQ54_12950"
     CDS             complement(<1..644)
                     /locus_tag="VFQ54_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0165947.1"
                     /translation="MSSPPSSSEPKVSAQDYAAAYARLHPKIQQWIWDQGWQQLRAIQ
                     ARAIDPVMEGESDVIISAATAGGKTEAAWLPIVSALGIERDQQTAEVGIKALYISPLK
                     ALINDQADRLRDLAAAVELPVYRRHGDVGGSDRTKVLREPDGILLITPESLEALFVHQ
                     GTRIPSLFAGLRYAVIDELHSFIGTERGAQLQSLLHRIELALRRHVPRIALSATL"
     gene            complement(619..>1996)
                     /locus_tag="VFQ54_12955"
     CDS             complement(619..>1996)
                     /locus_tag="VFQ54_12955"
                     /inference="COORDINATES: protein motif:HMM:NF022376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0165948.1"
                     /translation="TGAPDRIRPRDRDAIINSLRAGVVPRAGQQHIQVGRVSELNALL
                     SDIDRIVDDGSAARFVIGEYGSGKTFFLNLVRSVALQKKLVTVHADLSPDRRLHATSG
                     QARSLYAELMRNMSTRAKPDGGAMTSVVERFVTQALTEARTTGEAPEVVIHRGLAQLS
                     ELTGGYDFAEVIAKYWQGHDSGNEQLQADAVRWLRGEFATRTDARTALGVRTIIDDAN
                     FYDQLKLLALFVRLAGFDGLFVVLDEMVNLYKLANTQARNANYEQILRILNDATQGTA
                     IGIGFTFGGTPDFLLDTRRGLYSYAALQSRLAENSYARDGLIDYTGPIIRLANLTQED
                     FYVLLTKLRHIQAAGDPGAYLIPDAGIQAYMTHVAQRIGDAYFRTPRNTIKGFLDLLA
                     VLEQNPGRTWSDLIDSVEIDEDHNPDLEPIPATALDAAVNGAVPPTKPVAPTVPDDED
                     DELATFKL"
BASE COUNT          322 a          604 c          718 g          352 t
ORIGIN      
        1 agggtcgcgg agagcgcgat ccgcgggacg tggcgacgca gcgcgagctc gatccgatgg
       61 aggagcgatt gcagctgggc gccgcgttcg gtcccgatga aggagtgcag ctcgtcgatc
      121 acggcgtagc ggagaccggc gaagagcgac gggatgcgcg tgccctggtg aacgaagagc
      181 gcctccagcg attccggcgt gatgaggagg atgccgtcgg gctcgcgcag cactttcgtg
      241 cgatcggagc cgccgacatc gccgtgccgg cggtagaccg gcaactcgac ggcggcggcg
      301 agatcgcgga gacgatcggc ctggtcgttg atcagcgcct tgagcgggct gatgtagagc
      361 gccttgatcc cgacttcggc cgtctgctgg tcgcgctcga tgccgagcgc ggagacgatc
      421 ggcaaccagg cggcctcggt cttgccgccc gcggtcgcgg cgctgatgat gacgtcgctc
      481 tcaccttcca tcacgggatc gatcgcgcgc gcctggatcg cgcgaagctg ctgccagccc
      541 tgatcccaga tccactgctg gatcttcgga tgcaggcggg cgtacgccgc cgcgtaatcc
      601 tgggcggaaa ccttcggctc agagcttgaa ggtggcgagc tcatcgtcct catcatccgg
      661 tacggtcggc gcgaccggct tcgtgggggg aacggcgccg ttcaccgccg cgtccagtgc
      721 ggttgccggg atcggctcga gatcggggtt gtggtcctcg tcgatctcga ccgagtcgat
      781 caggtcgctc cacgtcctgc cgggattctg ctcgagaacg gcgaggagat cgaggaagcc
      841 cttgatcgtg ttgcggggtg tccggaagta ggcgtcaccg atccgctgcg cgacgtgcgt
      901 catgtacgcc tggatcccgg catcgggaat gagataggcg ccgggatcgc cggcggcctg
      961 gatgtgccgg agcttggtga ggaggacgta gaaatcctcc tgcgtcaggt tcgcgaggcg
     1021 gatgatcggt ccggtgtagt cgatgaggcc gtcgcgcgcg tagctgttct ccgcgagccg
     1081 ggattgcagt gcggcgtagg aatagagacc gcggcgggta tcgagcagga aatccggcgt
     1141 accgccgaag gtgaaaccga tcccgatcgc cgttccctgt gtcgcatcgt tgaggatgcg
     1201 gagaatctgc tcgtagttcg cattccgggc ctgggtgttc gccagcttgt agagattgac
     1261 catttcgtcg aggaccacga agaggccgtc gaacccggcg aggcggacga agagcgcgag
     1321 cagcttcagc tgatcgtaga aattcgcgtc gtcgatgatc gtacgcacac cgagcgcggt
     1381 gcgggcgtcg gtgcgggtcg cgaactcgcc gcgcagccag cgcacggcgt ccgcctgcag
     1441 ctgttcgttt ccgctgtcgt ggccctgcca gtatttcgcg atcacctcgg cgaagtcgta
     1501 gccgcccgtc agctcggaga gttgggcgag accgcgatga atcacgacct cgggggcctc
     1561 gccggtcgtg cgggcttcgg tcagcgcctg ggtcacgaag cgttcgacga cgctcgtcat
     1621 cgcgccgccg tccggcttcg cccgggtcga catgttccgc atcagttcgg cgtagaggct
     1681 gcgcgcctgg ccgctggtcg catgtaggcg gcgatccggc gagagatcgg cgtgaacggt
     1741 gaccagcttc ttctgcagcg cgacggagcg aaccagattg aggaagaacg tcttcccgga
     1801 cccgtactca ccgatgacga accgtgccgc ggaaccatcg tcgacgatcc gatcgatgtc
     1861 gctgagcagt gcgttgagct cgctgacccg accgacctga atgtgttgct gaccagcgcg
     1921 cggaacgacc ccggctcgca gcgagttaat gatcgcgtcg cggtcacggg gccggatgcg
     1981 gtccggggca ccggta
//
LOCUS       DASTMD010000873         1803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1778676, whole genome shotgun sequence.
ACCESSION   DASTMD010000873 DASTMD010000000
VERSION     DASTMD010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778676"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..792)
                     /locus_tag="VFQ54_12960"
     CDS             complement(58..792)
                     /locus_tag="VFQ54_12960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU0165949.1"
                     /translation="MTSKDQPAWDTEDRRLLSWSDKDRVQAAAFTHSDQWRVLRILGE
                     FVEGFDALAEIGPAVSVFGSARTPLDDPMSQSAYQLGHALAEAGIAVITGGGPGIMEA
                     SNRGAAEAHGISVGAGIELPFESGLNPYVTVPLRFRYFFVRKMMFVKYARGFIFFPGG
                     FGTLDELFEICTLVQTGKLDSPPIVLFGSSYWRGLLDWIDEELIGGGKVNPDDRSFFS
                     VTDSVTEATALLTAAFPKASDQQATE"
     gene            958..1452
                     /locus_tag="VFQ54_12965"
     CDS             958..1452
                     /locus_tag="VFQ54_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165950.1"
                     /translation="MLRRLQGGVTVLLVLATVACGSGGGDEPAVTRVPTFAATPSPVA
                     TPSTPVKMLDVVWATAAKADRGGPSRNLTSIPENAKTIYAFAMSGAIPAGSVLEAKWT
                     MNGIAIPGISQRVTVTEALPAGWVEFHLSWTGAQNWPQGTLAVTIVANGKVSSSGSIE
                     IVAT"
BASE COUNT          313 a          555 c          595 g          340 t
ORIGIN      
        1 cggtgcgagg cgattggtgt tgcccccggt ggcgcctgtc cgatcacccg ttcgacgtca
       61 ttcggtggcc tgctggtcac ttgccttggg aaaggcggcg gtgaggagcg cggtggcctc
      121 ggtcaccgaa tcagtcaccg agaagaacga cctgtcgtcg ggattgacct tgccgcctcc
      181 gatgagttcc tcgtcaatcc agtcgagcag gcctcgccag tatgaagaac caaaaaggac
      241 gattggcggg ctgtcgagct tgcccgtctg cacgagcgta cagatctcga agagctcgtc
      301 gagtgtcccg aatccacccg ggaagaagat gaatccccgg gcgtacttca cgaacatcat
      361 cttccggacg aagaaatagc ggaagcggag gggcacggtc acataggggt tcaggccgga
      421 ttcgaacggt agctcgattc ccgcgccaac cgagattcca tgagcctccg cggctccccg
      481 attgctggct tccatgatcc ccggcccgcc gccggtaatg acggcgatcc cggcttcggc
      541 gagcgcgtga ccgagctggt acgcggactg gctcatcggg tcgtcgagcg gcgttcgtgc
      601 cgatccgaat acgctcacgg cgggaccgat ttccgccaac gcatcgaagc cttcgacgaa
      661 ttcaccgaga atccggagca cccgccactg atcgctgtgc gtgaaggcgg ccgcctggac
      721 ccgatccttg tcgctccatg agaggagacg gcggtcctcg gtatcccacg ctggctggtc
      781 tttcgacgtc atgtggatcc tcctccgttg ccatgcggtt gtctcgatga ggataccgcg
      841 tggccggggt ggaggcggta caatcccgcg tggttgggac cgggagaagc cgatcgccgg
      901 ggcgagggga tcttgaagaa tccgcgcgga gacaatcgaa gaacgcgggt atcttccgtg
      961 ctgcgtcggc tccagggcgg cgtgacggtt ctgcttgtgc ttgccaccgt tgcgtgtgga
     1021 tccggcggcg gtgacgagcc ggcggtgacg cgggttccca ccttcgcggc gacgccgagc
     1081 cccgtggcga cgccttcgac gccggtgaag atgctggatg tcgtctgggc gaccgccgcc
     1141 aaggcggatc gcggcggtcc ttcccggaac ctcacctcca ttcccgagaa cgcgaagacg
     1201 atctatgcct tcgccatgag tggcgcgatc cccgcgggga gcgtgctcga ggcgaaatgg
     1261 acgatgaacg ggatcgcgat ccccggaatc tcgcaacgtg tcacggttac ggaagcgttg
     1321 ccggccggct gggtcgagtt ccatctgagc tggaccgggg cgcagaactg gccccaggga
     1381 acgctcgccg tgacgatcgt cgcgaacggg aaagtctcgt cgtcgggctc catcgagatc
     1441 gtggccacct gatcgctttt cctcgcgaca tcacggcatc cagcgggtat gatgcctcca
     1501 cgtttcaggt tcgaagatct ggggacaggc ggggcagcgg gctccgtggc gttcgtttac
     1561 cgtggtggag gcaatcgtgc tacctgtgca tcgttcgcaa cgcaactggg gggctcgtgt
     1621 ccgtgatgga catgtgaggg gcatctacac gatcgtcctc gagaacgagc gcattcgcgt
     1681 ttccgtgctc gccggcaagg gaaccgacat cgtcgagatc ctctacaagc cgctcgacat
     1741 cgacttcaac tggctgaccc cgggcgggat tcgcaatccg acctcctatc tctcgtcgtc
     1801 tcc
//
LOCUS       DASTMD010000874         5244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_349553, whole genome shotgun sequence.
ACCESSION   DASTMD010000874 DASTMD010000000
VERSION     DASTMD010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349553"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /locus_tag="VFQ54_12970"
     CDS             complement(<1..1802)
                     /locus_tag="VFQ54_12970"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEU0165951.1"
                     /translation="MKDVLLERLAALMVFVTLVGSGGQIALGQTPEASPAATPFASPV
                     ASPAAAAKHGIKIEDMDLSVDPGEDFYQFANGGWLASTELPSDSPRYGTFDEIDDLVT
                     GQLDTLVADLPADPSTDTGKVKTVYNQALDMATRDAQGVAPIRPLLDRFGKIATIDDG
                     IAYQEKEARLDGMQGILNIYGGPSFTDATKNVAYLGSPVLSLPSLDFYLDDSADYESI
                     RQAWVETTAKLLVQAGYSEADATSAAKQVLAFETAEAKIMTTPQEYSADPSLYSTETS
                     VADLEKLFPEFYFRAWLDTLGLTGVDTLAVDDIKFFTGLAAVLKAAEPTTIGNLLAVE
                     LLWSAAPYLTTEIGNIAFSFNGPILNGVSVRRPANERALTLVETYFPDAVSQAYVAEA
                     FPPSAKAQIEDLVKNIIAAYRQRILSSTWMSDETKQKAVTKLDLISVKIGYPDKWISY
                     ATVPVGDSLIETINNAANFANARNLSEIGKPVDRTEWSMGAFEVNAYYNPQGNEIVFP
                     AAILQPPFFDPEADAASNYGAIGAVIGHEITHGFDQSGSQFDGYGNLNSWWTDQDRAA
                     FDALNQKVIDQYDQLEALPGLYVDGELTVTENVA"
     gene            complement(2278..2970)
                     /locus_tag="VFQ54_12975"
     CDS             complement(2278..2970)
                     /locus_tag="VFQ54_12975"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEU0165952.1"
                     /translation="MYFQHAEDIWRDFPELVPMTLFAEGITPDVDVRERIAPFNATAT
                     ERLAISSEGEFPEIQAWRRAFSRMGLKPTQYRCASESLLRRFRKDGALPEIHPLIDLC
                     NAISLAYAIPIAVFDLARVRDFIEVRRATGEEVYLTLGGETEHPDPNEVIFADGAGNA
                     HARRWTNRQSGASAIRESTSSILIVAEAMHSAAADDVPRLLATISAELESVWPVSPRS
                     AILAPSASRFEF"
     gene            complement(3215..4570)
                     /locus_tag="VFQ54_12980"
     CDS             complement(3215..4570)
                     /locus_tag="VFQ54_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165953.1"
                     /translation="MIRRLRFRFGSRVRVGVIAFIALVLALAPSLRGAFAQVIDPNQH
                     AEVVAQGVGTMPTGQVVWRVTQGVAPQASASQQSTYPLGFVYVATGTMQIGNSGTGNR
                     VLLQDGEASFQADGAQQRRKSATTAAVPYLGIELATAAQANTASPNNTLLTTSDAFAA
                     PSGDRDVNLVRDVLADNESGGLPGSDNPTYVYVTIGAITVTPAGGSATTLNAGQGQLF
                     TGALQIQAGQNGGTYLAAGIGAQATSDNLGGGNGSGSGSGSGNGNGNGSGNGSGNGNG
                     NGNGSGGGNGNGSGGGNATAIAVTPTNPVTGTGELRITVYLCPAGSNPPSGDSDDCDI
                     TTGDLNGNPVPNGVFWTYPDMATETALLNAPVQDGTTFDWPQMPNGHYDLDQDSFLQG
                     LDYQIYYWASYHHPTGDQVSGDTGPFSVSSNNTTDVYVYLYPTLTVTPTVETTPDATP
                     V"
     gene            4987..>5244
                     /locus_tag="VFQ54_12985"
     CDS             4987..>5244
                     /locus_tag="VFQ54_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165954.1"
                     /translation="MNARNHDQIEGYLLTDDEQRIAARTVPIGAPPVREAVDDHAAEI
                     LQIERARRDPIDFAPLYERYVDAVHGYCARRVTDPELAADLT"
BASE COUNT          952 a         1640 c         1698 g          954 t
ORIGIN      
        1 tccgcgacgt tctccgtcac cgtcaactcg ccgtcgacgt agaggccggg caacgcctcg
       61 agctggtcat actggtcgat caccttctgg ttcaacgcat cgaacgcggc gcgatcctga
      121 tccgtccacc acgagttgag attgccgtag ccatcgaact gcgaaccgga ctggtcgaag
      181 ccgtgcgtga tctcgtgacc gatcacggcg ccgatcgccc cgtaattcga ggctgcgtcc
      241 gcctccggat cgaagaacgg cggctgcagg atcgccgccg ggaatacgat ctcgttcccc
      301 tgcgggttgt agtaggcatt cacctcgaat gcgcccatcg accactcggt gcggtcgacc
      361 ggcttcccga tctcggagag attccgcgca ttcgcgaagt tcgccgcatt gttgatcgtc
      421 tcgatcagcg agtcaccgac gggaacggtc gcgtagctga tccacttgtc cgggtaaccg
      481 atcttcaccg agatcagatc gagtttcgtg acggccttct gcttcgtctc gtcgctcatc
      541 caggtcgaac tcaggatgcg ctggcggtag gcggcgatga tgttcttgac cagatcttcg
      601 atctgcgcct tcgcggacgg cgggaacgcc tcggcgacat acgcctggct caccgcatcc
      661 gggaaatagg tctcgacgag cgtcagcgcc cgctcgttcg ccgggcggcg gacggagacg
      721 ccgttcagga tcgggccgtt gaaggagaag gcgatattgc cgatctcggt cgtgagatac
      781 ggcgcggcgc tccagagcag ttcgaccgcg agcaggttgc cgatcgtcgt cggctccgcc
      841 gccttcagca cggcggcgag cccggtgaag aacttgatgt cgtcgacggc gagcgtgtcg
      901 acgccggtga gaccaagggt gtcgagccag gcccggaaat agaactccgg gaagagcttc
      961 tccagatcgg cgaccgacgt ctccgtgctg tagagcgacg gatccgccga gtactcctgc
     1021 ggcgtggtca tgatcttcgc ttcggcggtc tcgaaggcga ggacctgctt cgcggcggac
     1081 gtcgcatccg cttcgctgta tccagcctgg accagcagct tcgcggtcgt ctcgacccac
     1141 gcctgacgaa tcgattcgta gtccgcggag tcatcgaggt agaagtcgag actcggcagg
     1201 ctgagcaccg gactgccgag ataggcgacg ttcttcgtgg catcggtgaa ggaaggtccg
     1261 ccgtagatgt tcagaattcc ctgcatgccg tcgaggcgcg cttccttctc ctgataggcg
     1321 atgccgtcat cgatcgtcgc gatcttgccg aagcgatcga gcaacggccg gatcggcgcg
     1381 acgccctggg cgtcacgcgt cgccatgtcg agcgcctggt tgtagaccgt cttcaccttc
     1441 ccggtgtccg tgctcgggtc cgccgggaga tcggccacca gcgtgtcgag ttgcccggtg
     1501 acgaggtcat cgatctcgtc gaaggtgccg tagcgcgggg agtcagaagg aagttcggtg
     1561 ctggcgagcc agccgccatt ggcgaactgg tagaagtctt ccccgggatc gaccgagaga
     1621 tccatgtcct cgatcttgat gccatgcttc gccgccgccg ccggggatgc gaccggcgag
     1681 gcaaacgggg ttgccgccgg tgacgcctcg ggcgtctggc cgagcgcgat ctggcctccc
     1741 gagccgacaa gcgtcacgaa aaccatcaac gccgcgaggc gttccaagag aacgtccttc
     1801 acgaagacct ccccataccg gcgacacacc ggacgacgac ctgacgaacg aacgatggtg
     1861 acgcggcgtg cgtaatgccc gccggaccgg gatggagcga tgacggattt gtcactactc
     1921 taccagagcg ttgcgtcaat tcagatcgat tttcgttgaa ggaagtacca ggatctcaat
     1981 acgcccggcc catgccaacc ccgatggtgg tcaccgggat ggaaccgaca cgctcatttc
     2041 aggcacgttc cgcatatgtc gtgggaggtg gcaacaaggg ccagcgggac ggagcactgt
     2101 ggaatcgtgc aggcgacgcg ccacgaatgg gagattgggc gatcacgaga gtcgccccta
     2161 cgggattacc gaccttcgta ggggcgaccc tggtggtcgc cctgcccaac ctctggggat
     2221 cacgtcacgc ccggcccgac gccggccggg ctcttctgaa tgacgtctat gaggaggtca
     2281 gaactcgaag cgggacgccg agggtgccag gatcgccgag cgcggggaga ccggccagac
     2341 ggactccagt tccgccgaaa tcgtcgcgag gagccgcggc acatcgtcag cggcggcgga
     2401 atgcatcgcc tcggcgacga tcaggatcga ggaggtcgac tcccggatcg cggaggcgcc
     2461 gctctggcgg ttcgtccagc ggcgcgcgtg ggcgttgccc gccccgtccg cgaagatcac
     2521 ctcgttcgga tccggatgct cggtctcgcc accgagcgtg aggtagacct cctcgccagt
     2581 cgcccgccgt acctcgatga aatccctcac cctggcgaga tcgaagacgg cgatcggaat
     2641 cgcgtaggcg agggagatcg cattgcacag atcgatcagc ggatggattt ccggcaacgc
     2701 gccgtccttc cggaagcgac gcagcagcga ttccgaggca cagcgatact gcgtcggctt
     2761 cagtcccatc cgcgagaagg cgcgccgcca cgcctgaatc tccgggaact cgccttcgga
     2821 cgatatcgcg agacgctccg tcgcggtcgc gttgaacggc gcgatccgct cacggacatc
     2881 cacgtctggc gtgatgccct cggcaaagag cgtcatcgga acgagttccg ggaagtcgcg
     2941 ccagatatcc tccgcgtgct gaaagtacat cgtgacgctc ctcctcgggt ggctggatgc
     3001 ggtgcgtgat aggaatgata tcggaagcgg tcgtacgtgg ggtgggactg cggaatcgca
     3061 agaccgtcag tccgggcgcc ctgcgagagt gatgtccggt ccaatgacgt cggcgtaggg
     3121 tgccgcaaca gcgcgacggc cgggtctggt ggacccggcc gtcgttcgtg ctgttcgtcc
     3181 ctgatgccat gcgaccgtcc cgggccgcgc gttcctacac cggcgttgcg tccggtgtcg
     3241 tctcgacggt cggcgtcacc gtcaacgtcg gatagagata gacgtagaca tcagtcgtgt
     3301 tgttcgagct gaccgagaag gggccggtat cgccagaaac ctggtcgccg gtggggtggt
     3361 ggtagctcgc ccagtagtag atctgatagt ccaggccctg caggaaggag tcctggtcaa
     3421 ggtcatagtg gccgtttggc atctgtggcc aatcgaacgt cgtgccgtcc tgcaccggcg
     3481 cgttcaggag cgccgtttcc gtcgccatgt ccgggtaggt ccagaagaca ccgtttggta
     3541 ccggattgcc gttcagatct ccggtcgtga tgtcgcaatc gtcactgtcg ccggagggcg
     3601 gattggaccc ggctgggcac aagtagacgg tgatgcggag ttcgcccgtc ccggtgaccg
     3661 gattcgtcgg ggtcaccgcg atagccgtgg cgttgccgcc gcctgatcca ttgccgttgc
     3721 cgccaccgga gccgttgcca ttgccattgc cattgccgga accgttaccc gagccgttgc
     3781 cgttgccatt gccgctaccg gagccactgc ccgagccgtt accgcccccc agattgtccg
     3841 atgtcgcctg ggcaccgatt ccggctgcga ggtaggtacc gccattctgg ccggcctgga
     3901 tctgaagcgc gccggtgaac agctgccctt gcccggcgtt gagggttgtc gcggaaccac
     3961 cagccggtgt cacggtgatg gcgccaatgg tgacgtagac atacgttggg ttgtccgagc
     4021 caggaagccc gccagactcg ttatccgcca gcacatcccg gacgagattg acgtcgcgat
     4081 cgcctgacgg tgcggcaaag gcatcgcttg tggtgagcag ggtgttattg ggtgatgcgg
     4141 tattggcctg ggcagcggtc gccagctcga tgccaagata gggcacggcg gcagttgtcg
     4201 cgctcttgcg ccgttgctgc gcgccatcag cctggaaact cgcctcgcca tcctgcagga
     4261 ggacgcgatt gccagtgccc gagttgccga tctgcatcgt gccggtcgcc acgtagacga
     4321 agccaagcgg gtaggtcgat tgttgggagg cgctcgcctg gggcgcgacc ccctgggtga
     4381 ctcgccagac aacctgtccg gtcggcatgg tgccaacacc ttgcgcgacc acctcggcat
     4441 gctgattggg atcgatcacc tgcgcgaagg caccccgcag cgacggagcg agagccagga
     4501 ccagggcaat gaacgcgatc acgccaactc gcacgcggga cccgaatcga aaacgcaaac
     4561 gccgaatcat gatcatctct ccctgtatgt ggggccgcgg aagcccaagc cactacgatg
     4621 acacaacggg caacattcca aatgcccaac ttcaccgcga tgccacttat tctggacacc
     4681 cgcaatcccg cagtcgttag tagaaaataa gtattccggc ggttcgggag caccatttcc
     4741 cgtacatatg ccttcaagag cgcgaatcaa cgattgagta cggcggcttg cgggaacttc
     4801 atggcatccg cctgtcgatc gttggtcaga aatcaccacc gctatactgg gcgcgatggg
     4861 gacgagaatc gatcctgcct tgccgcgaat ccttttgtgc gaccgcacgt tgacctctgt
     4921 gtgcggatca ggcaggatcg tccagggaga gatcgaccat cgcatcagca acgggaatgg
     4981 cttcgggtga acgcgcgcaa ccacgaccag attgagggat atctccttac ggatgacgag
     5041 cagcggatcg ccgcgcgtac cgtcccgatc ggcgcgccac cggtgcgcga ggccgtcgac
     5101 gaccacgccg cggagattct ccagatcgaa cgcgcgcggc gggatccgat cgacttcgca
     5161 ccactctacg aacgctacgt cgacgccgtg cacggatact gcgcgcgccg ggtcaccgat
     5221 cccgagcttg ccgccgatct gacg
//
LOCUS       DASTMD010000875         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_168529, whole genome shotgun sequence.
ACCESSION   DASTMD010000875 DASTMD010000000
VERSION     DASTMD010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168529"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VFQ54_12990"
     CDS             <1..840
                     /locus_tag="VFQ54_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0165955.1"
                     /translation="AAKHDLSSLRLLGSVGEPINPEAWIWYHKYIGAERAPIVDTWWQ
                     TETGSILISPLPGLTTTKPGSATLPLPGIDVDIVNEAGESVPFGGGGYLVIRKPWPSM
                     LRGIYGDPDRYASTYWSKYDGIYFPGDGAKRDEDGYFWLLGRVDDVMNVSGHRLSTTE
                     IESALVSHDLVAEAAVVGSPDPLTGEAIFAFVTLKSRAHVTPDFGDELRQYVATKISP
                     IAKPKRLMFTPDLPKTRSGKIMRRLLRDIAGGHQLGDTTTLADPSIVEQIRDASLSAK
                     DDD"
     gene            1642..2787
                     /locus_tag="VFQ54_12995"
     CDS             1642..2787
                     /locus_tag="VFQ54_12995"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEU0165956.1"
                     /translation="MKRSAVSLSALVLSAACALSPLVSMAQTPAATPAPVDLSSVRII
                     GTTTIPNDLMVDGTLVGGLSGIDYDAATGKYVIISDDRSDNQPARFYNADVPVTKDGI
                     GPVKIDSAVTFEQSNGTPFPNQTVGGNVPDPEAIRIDPNSGDIFWTSEGSQARLINPF
                     IAVAAPDGKFIASPQTPPIFDMSSTGLTGPRDNLAFEGLSFSTDGNSLWLATEGPLYQ
                     DGPVASATSGAPTRITNLDRSGNILGQYAYELDPLPIAPVAGGFSVAGVSEILAIDAH
                     RFITIERTSVQAEGGVVNNYLKLYEIDINGATDVKSQQWLTQGGYTPVTKKLILNFNE
                     TDVTVDNFEGITWGPTLDDGNRTLIVESDNNFADNQIQEFVALEVQS"
     gene            complement(2991..3134)
                     /locus_tag="VFQ54_13000"
     CDS             complement(2991..3134)
                     /locus_tag="VFQ54_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165957.1"
                     /translation="MQKLLDRYRELSGIYAFGFDKASFLGVALPLIIVIVVGGAGAWI
                     VTH"
BASE COUNT          603 a         1107 c         1038 g          599 t
ORIGIN      
        1 gcggcgaagc acgatctctc gtcgctgcga ctcctggggt cggtcggcga gccgatcaac
       61 ccggaagcgt ggatctggta ccacaagtac atcggtgccg agcgcgcccc gatcgtcgat
      121 acctggtggc agacggagac gggctcgatc ctgatctcgc cgctgccggg gctgacgacg
      181 accaagccgg gatccgcgac gctgccgctg ccgggtatcg atgtcgacat cgtcaacgag
      241 gcgggcgagt cggtcccctt cgggggcggt ggttacctcg tgatccgcaa gccgtggccg
      301 tccatgctcc gtggcatcta cggcgatccg gatcgctacg cgtcgactta ctggagcaag
      361 tacgacggga tctacttccc gggagatggc gccaagcgcg acgaggacgg ctacttctgg
      421 ctcctcgggc gtgtcgacga cgtgatgaac gtatcgggtc atcgcctgag cacgaccgag
      481 atcgagagcg ccctcgtctc gcacgatctc gtcgccgagg cggcggtcgt cggatcgccg
      541 gatccgctga cgggcgaggc gatcttcgcc ttcgtgacgc tgaagagccg cgcccatgtc
      601 acccccgatt ttggtgacga gctgcggcag tatgtcgcga cgaagatcag tccgatcgcg
      661 aagccgaagc gcctgatgtt cacaccggac ctgccgaaga cgcgatcggg caagatcatg
      721 cgtcggttgc ttcgcgatat cgcgggtggg catcagctcg gtgacacgac gacgctggcc
      781 gatccgtcga tcgtcgagca gatccgtgac gccagcctct ccgcaaagga cgacgactag
      841 tctcgggtcg tcgtgtcgcc gcgagcagga tcctcgagga cgggaggcgg gacaggccgt
      901 ccggacctcc ggtccggcag cggcggtccc gccctacgaa cgaccggacg ccgtgccgga
      961 ggtcgcggcc gaccggcggc atgtgtggcc gtcgctgtag tcgcgactcg taatttggtt
     1021 cggcccacga gcgtccggga ggaccttcct cccgggcgcc ttttattcgc gcccctccat
     1081 gcctccggtc gaacatcgtc cgatggcacg aatcgccatc gttacacaac ctcgtagggg
     1141 cgaacctggt gttcgccctg atcgtcgata ccacgcgtgt tccctgcgag acccgccagg
     1201 acacgtcctc ccgagcgtgt cgagggatca tctggtcacc tatgtgcttc cctcacgacg
     1261 ccatacaaga cacgtcctcc cgagcctgtc gagggatcag ccggcgtcca cgtgcttccc
     1321 atccgacgtg cttccccaag agaggcaggc agccgaggcg tcaccaatgg gacgacccgg
     1381 gcgatcacga ggatcgcccc taccgatgcg cgcctggcgg cggcgcgtcg tcgttccgcg
     1441 cgaaatgacg ggttggaggc gttgcgtcac ggtgaagcgg cggtcgtcga atccgatgtt
     1501 gctaacacaa cgcaatatct ccctagtttc gcgttaatac ctttcccctt cgcgatcgct
     1561 attcatagat gagcgctcag tgggattggg gatcgcctcg attggctgga cgcacggcat
     1621 tcatctggat ggaggcaagt catgaagcga tcggcggttt ccctttccgc gctcgtcctc
     1681 tccgcggcgt gcgcactcag ccctctggtt tcaatggcac agaccccggc cgcgacgccg
     1741 gcgccggttg atctcagcag cgttcgtatc atcggcacga cgacgattcc gaacgacctg
     1801 atggtcgatg gcacgctcgt cggcggcctc tccggcatcg actacgacgc ggcgacgggc
     1861 aagtacgtca tcatcagcga cgaccgttcc gacaaccagc ccgcgcgctt ctacaatgcc
     1921 gacgtgcccg tcaccaagga tggcatcggc cccgtcaaga tcgacagcgc cgtgaccttc
     1981 gagcagtcga atggcacccc gttcccgaac cagaccgtcg gcggcaacgt tcccgatccg
     2041 gaagcgatcc gcatcgatcc gaattccggc gatatcttct ggacgagcga gggcagccag
     2101 gcccgcctga tcaacccgtt catcgcggtt gcggcgccgg acggcaagtt catcgccagc
     2161 ccgcagacgc cgccgatctt cgacatgtcc tcgaccggtc tcaccggccc gcgtgacaat
     2221 ctcgccttcg agggactcag cttctcgacg gacggcaact cgctctggct cgcgacggaa
     2281 ggtccgctct accaggacgg cccggtcgcc agcgccacga gcggcgcgcc gacacgcatc
     2341 accaatctcg atcgctcggg taacatcctc ggtcaatacg cgtacgagtt ggatcctctg
     2401 ccgatcgccc cggtcgccgg cggcttctcc gtcgccggtg tttccgagat cctcgcgatc
     2461 gacgctcacc gcttcatcac gatcgagcgc acgagcgttc aggccgaggg tggcgtcgtc
     2521 aacaactacc tgaagctcta tgagatcgac atcaacggcg cgaccgacgt gaagtcgcag
     2581 cagtggctaa cgcagggtgg atacaccccg gtcaccaaga agctgatcct caacttcaac
     2641 gagactgatg tcaccgtcga caacttcgag ggcatcacct ggggcccgac gctcgacgat
     2701 ggcaatcgca ccctgatcgt ggagtctgac aacaacttcg ccgacaacca gatccaggag
     2761 ttcgtcgcgc ttgaggttca gtcctagagc gactgctgga accgtcgccc ccgggagcac
     2821 gatccgcggg cagtcccgcc tccgggacga ccgggtgtcc ggtggaacac cgcatctggt
     2881 cgcgggtgca tggggcgttc ccgatagttg gaacggcaga tacgcaggaa gggccgggat
     2941 ggcaacgacg ccatcccggc ccttcctgcg ctttcaatcc ggatcaaaga tcagtgcgtg
     3001 acgatccatg ccccggcgcc accaaccacg atcacgatga tgagggggag tgcgacgccg
     3061 aggaacgacg ccttgtcgaa tccgaatgca tagatcccac tgagttcgcg gtatcgatcg
     3121 agcagtttct gcatgacatc taccctcgac aactcgtgcg tctggtcgct cgcgcacggc
     3181 tgcgcaccag actggctctt tgattgaggt tcgttgtcgc agagtgtaca tcagcgatcg
     3241 gtgaatcgcg cgaacgggct agaggtcgtc ggccttgacc tcgtcgtcag cttcggattc
     3301 gccgctcgcg agctggcgca tcgccgggaa gaggatcacg tcgcgca
//
LOCUS       DASTMD010000876         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2046120, whole genome shotgun sequence.
ACCESSION   DASTMD010000876 DASTMD010000000
VERSION     DASTMD010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046120"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VFQ54_13005"
     CDS             <1..1059
                     /locus_tag="VFQ54_13005"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU0165958.1"
                     /translation="MLDYWRANGSAAVYGNPISEPFAAPNGLYSQAFERGIFQWNPNW
                     LNTEDPTVRLMPIGADVLSQRQGTLTAAGRRTGADPRLTAFTPPSSNSARAEVVNSLN
                     GRFSTVTGFSIAGDYQEWYDDHEGTFYLGEPISEPFSESGQVVQYYENGMLYETGSGV
                     QLAKLPTLNPAQWGFDTTPIKQGDLQTYDESIFQTADNPGGVDVTQLSGKRFIQVTLS
                     TQSMGVYQGGDTVLTSLISSGLDPNFTVPGEFHVRLKFPSQTMAGFTDESGETAGFRD
                     PNDTNSADTPTGDTPWLVPDVPNVMYFSLSAEALHGCYWHSNFGNPMSHGCINLPLDV
                     AAFMYGWNPLGVHVSIIS"
     gene            1326..2132
                     /locus_tag="VFQ54_13010"
     CDS             1326..2132
                     /locus_tag="VFQ54_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0165959.1"
                     /translation="MLAFANQKGGVGKTTSAVNTAAILSRRGFRTLLIDIDPQGNSTS
                     SLGVDKTSLTGSTYDVLVDEIGIAESVVSSVRENLDLLATTPTLAAAEVELVNAGNRE
                     HRLQVAVRALGSAYDAILIDCPPSLGMLTVNALTASDSVIVPIQCEFLALEGVSQLIT
                     TIDLVKRQLNPDLDILGILMTMYDSRTRLSAHVVAEVRRYFPDRIFTTVIPRSVRLAE
                     APSYGQSILEYDATSRGGDAYEAFVSELSTRAALVPHRSEEVRLATTTAT"
     gene            2201..>3007
                     /locus_tag="VFQ54_13015"
     CDS             2201..>3007
                     /locus_tag="VFQ54_13015"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU0165960.1"
                     /translation="MSQTLTPPHVSEHIDGAEPSVDVSMTSAMRTDKFVGLGLTFDDV
                     LLVPAASGVIGLDVSTKTRLIRDVTLNVPLVSAAMDTVTEARLAIALAREGGIGVVHR
                     NLSIDDQAAEIDKVKRSESGMIVEPVTLGPDRPVAAALAVMERYHISGVPITDENNRL
                     VGILTNRDLRFIENTSQPISDVMTSENLVTAPTGTSLEEAEAILHRHKVEKLPVVDED
                     GQLTGLITVKDIQKRIQYPNATKDARGRLRVGAAVGVGKDAEDRAEALVAR"
BASE COUNT          555 a         1064 c          853 g          535 t
ORIGIN      
        1 atgctcgact actggcgcgc caatggtagc gccgccgtat acggcaatcc gatctccgag
       61 ccgttcgccg ctcccaacgg tctctacagc caggcgttcg agcgcggcat ctttcagtgg
      121 aaccccaact ggctgaacac cgaggatccg acagtccggc tgatgccgat cggcgccgac
      181 gtcctcagcc agcgccaggg aaccctgacc gccgcgggtc gccgcactgg cgccgatccc
      241 cgcctcactg ccttcacgcc gccgtcctcg aattccgccc gcgccgaggt cgtcaactca
      301 ctcaacggtc gcttcagcac cgttaccggc ttcagcatcg ccggcgacta ccaggagtgg
      361 tatgacgatc acgagggtac cttctacctg ggtgaaccga tcagcgagcc attctcggag
      421 tcggggcagg tcgttcagta ctacgagaac ggcatgcttt acgagacggg cagcggcgtc
      481 cagctggcga agctgccgac gctcaacccg gcgcagtggg gcttcgacac cacgccaatc
      541 aagcagggtg acctgcagac gtacgacgag tcgatcttcc agaccgccga caatccaggc
      601 ggcgtcgacg tcacgcagct ttccggcaag cgtttcatcc aggtcacgct gagcacgcag
      661 agcatgggcg tctaccaggg tggcgacacc gtcctcacct cgctgatcag cagcggactc
      721 gatccgaact tcacggtgcc gggtgagttc cacgttcgcc tcaagttccc gtcccagacg
      781 atggcgggat tcacggacga gtccggcgag acagccggct tccgcgaccc aaacgacacc
      841 aactcagccg acacccctac gggcgatacc ccctggttgg tgccggatgt gccgaacgtg
      901 atgtacttca gcctcagcgc ggaagcgctc cacggctgct actggcacag caacttcggc
      961 aatccgatga gccacggctg catcaacctc ccgctcgatg tcgcggcgtt catgtacggg
     1021 tggaatccac tcggcgtgca cgtctccatc atctcctaga ctcgcgtccc ggaacgtcga
     1081 aacgcgatcg gggccttgcc agttttggca gggccccgat cacatttccg ggtcacaatg
     1141 attgagcgaa ctccgttcgc ctgttaccat catgtgatac cttggcggat tcgttccgcc
     1201 ggtcgtcgtc gtgacgagcg ttccggtcgc gatgccccat tccacgtcac tgggatccct
     1261 tgaataccac gtccacgtcc tcaaccgaaa tccatcgccc atccggggag gctcgcccgt
     1321 cccgtgtgct ggccttcgcc aatcagaagg gcggcgtcgg gaagacgacg agtgccgtga
     1381 atacggccgc gattctctcc cggcgaggtt ttcggacact gttgatcgac atcgaccccc
     1441 agggaaactc gacgtcctcg ctcggcgtcg acaagacctc cctcaccggt tccacctatg
     1501 acgtcctcgt cgacgagatc ggcatcgcgg agagcgtcgt ctcctcggtt cgcgagaacc
     1561 tcgatcttct cgcgacgacg ccgacgctgg ccgccgccga ggtggagctc gtcaacgccg
     1621 gcaatcgcga acatcgactc caggtcgccg tccgcgctct cggtagtgcc tacgacgcga
     1681 ttctcatcga ttgtcctccc agcctcggca tgctcacggt caacgcactg acggcatccg
     1741 actccgtaat cgttccgatc cagtgcgagt tcctcgccct cgagggcgtc agccaactga
     1801 tcacgacgat cgacctcgtc aagcgccagc tcaatcccga cctcgacatc ctgggcatcc
     1861 tcatgacgat gtacgacagc cgcacccgcc tctcggccca cgtcgtggcc gaggtccggc
     1921 gctacttccc cgaccgtatc ttcacgaccg taatcccccg ctccgtccgt ctcgccgagg
     1981 cgcccagcta cgggcaatcg attctcgaat acgacgccac atccaggggc ggcgacgcct
     2041 acgaggcgtt cgtcagcgaa ctgtcgacgc gcgccgctct cgttccccat cgctcggaag
     2101 aagtccggct ggcaacaacc accgccacct gacgcgtatt cgacccggta ctgattcgtt
     2161 acgtttcttc gtatccccat tcatggaggt ttttcggcag atgagtcaga cactcacccc
     2221 accccacgta tccgaacata ttgacggggc cgagccgtcc gtcgacgtct cgatgacatc
     2281 ggccatgcgc accgacaagt tcgtcggcct cggcctgacg ttcgacgatg tcctcctcgt
     2341 tcccgccgca tccggtgtga tcggtctcga tgtctcgaca aagacccggc tgatccggga
     2401 tgtcaccctg aacgtgccac tcgtctccgc ggcgatggac acggtgacgg aagcacggct
     2461 cgcgattgcc ctcgcccgcg agggcgggat cggcgtcgtg catcgcaatc tctcgatcga
     2521 cgaccaggcc gccgagatcg acaaggtgaa gcgatccgaa tccggcatga tcgtcgagcc
     2581 ggtgacgctc ggccccgacc gccccgtcgc cgcggcactg gccgtgatgg agcggtacca
     2641 catctcgggt gtgccaatca cggacgagaa caatcgcctc gtcggcattc tcaccaaccg
     2701 cgatctccgc ttcatcgaga acaccagcca gccgatctcc gacgtcatga cgagcgagaa
     2761 tctcgtcacc gcgccaaccg gcacctcgct cgaggaagcc gaagcgatcc tccaccgcca
     2821 caaggtcgag aagctcccgg tcgtcgatga ggatggccag ctgaccggcc tgatcacggt
     2881 gaaggacatc cagaagcgca tccagtatcc gaatgcgacc aaggacgcgc gtgggcggct
     2941 tcgcgtcggc gccgcggtcg gggtcggcaa ggatgccgag gatcgcgccg aggccctcgt
     3001 cgcccgt
//
LOCUS       DASTMD010000877         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2315765, whole genome shotgun sequence.
ACCESSION   DASTMD010000877 DASTMD010000000
VERSION     DASTMD010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2315765"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFQ54_13020"
     CDS             complement(<1..866)
                     /locus_tag="VFQ54_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165961.1"
                     /translation="MIDTDPVNHAVPTPETLQLWADELIPGDVWYWDDSPLELDVTGC
                     QVMNREAYRSDPTYSDIRFMNAYCEWQMPAEATQVIVSDRTWVGKLSVADRQRVVRMQ
                     VSLKRGLVFSADWFGELPAQLQEFVVADQVVLGQAVWASLPTAIQHRALMAEQRRWDD
                     LDCYPVPDGAPAHVQAIANMFSPHEGTNCFAITAYGANADSHLLATWLTVSPIEIVAP
                     LGYRQVPDVEPIADDVVIFEKEGKVIHAVWCAGHDRFLNKNGQSRFNPIRIVDWKMLD
                     TDWPDTTWKIYR"
     gene            complement(877..2511)
                     /gene="guaA"
                     /locus_tag="VFQ54_13025"
     CDS             complement(877..2511)
                     /gene="guaA"
                     /locus_tag="VFQ54_13025"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU0165962.1"
                     /translation="MTAQDITPASPILDETASPSIDPGEASAVRAAVDTVVVLDFGSQ
                     YSQLITRRIREVGVYGELLHHDVSWDEVAKLDPKAVILSGGPASVYEDGAPQLPKWVL
                     EQDLPVLGICYGMQLLAYNLGGEVAPASRREYGPAILDVEATGKKNPLFANVPEHMNV
                     WMSHGDHVTRIPDDFEVLATSANSPAAAMAKGQIVGIQFHPEVQHTPQGAEILRNFLV
                     DIAGIRADWSPESFIDTAIREIQEKVGDGRVLLGLSGGVDSSVAAALLHRAIGDQLTP
                     VFVNNGLLRLGEAELVQEVFARSFGMKLVYVDATERFLTKLADITDPEQKRKIIGNEF
                     VRVFEAEAEKAGPFEFLAQGTLYPDVIESTTKDTKAAAKIKTHHNVGGLPDDMQFGLI
                     EPLKFLFKDEVRAVGRSLGLPDEIVERQPFPGPGLSVRTMSAVNEPDLDLLRRADAIV
                     REEIEEAGLAKDVWQYFAVLLPVRSTGVMGDYRTYGRVCAVRAISSQDAMTADWARLP
                     YDVLARISNRIVNEVNGINRVVYDISSKPPSTIEWE"
     gene            complement(3124..>3635)
                     /locus_tag="VFQ54_13030"
     CDS             complement(3124..>3635)
                     /locus_tag="VFQ54_13030"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEU0165963.1"
                     /translation="QRLGNLGRVARPSRDTRMNRPTPVTLSLTLPLPPGVNNQYVTVG
                     KRRVLSKTAQTFKRDATKLIAGMREKGRIDPVQEQVIQKSLLGIYLTFYFETPMRRDL
                     DGGLKIALDVLATNLGFDDRSVVDLHLTKQIDPLHPRLEIELETIHDWTFDKEYVYLG
                     GSDESADPE"
BASE COUNT          659 a         1105 c         1213 g          658 t
ORIGIN      
        1 ctgcggtaga tcttccaggt agtgtctggc caatcggtat cgagcatctt ccagtcgacg
       61 atgcggatcg gattgaaccg cgattgaccg ttcttgttga ggaaacggtc gtgaccggcg
      121 caccagacag cgtggatgac cttgccttcc ttctcaaaga tcacgacgtc gtcagcgatc
      181 ggttcgacgt cgggcacctg gcgatagccg agaggcgcga cgatttctat gggtgatacc
      241 gtcaaccagg tcgccagaag atgggaatcc gcatttgcgc cataggccgt gatggcaaag
      301 cagttcgtgc cctcatgggg actgaacatg ttggcaatcg cctggacgtg cgcgggcgcg
      361 ccatcgggca cgggatagca atccaaatcg tcccatcggc gttgctcggc cataagcgcg
      421 cggtgctgga tggcggtcgg cagactggcc cagacggcct gaccgagcac gacctggtcc
      481 gccaccacga actcctgaag ctgcgccgga agttcgccga accagtcagc cgaaaacacg
      541 agcccccgtt tcaggctcac ctgcatccgg acgacccgct gccgatcggc gacggagagc
      601 tttccgaccc acgtccgatc gctgacgatg acctgcgtgg cttcggccgg catctgccat
      661 tcacagtagg cattcatgaa gcgaatgtcg ctgtaggtgg gatcggaccg gtacgcctcg
      721 cggttcatga cctgacaccc ggtgacgtcc agctcgaggg gcgaatcgtc ccagtaccag
      781 acatcgcccg ggatcagttc gtcggcccac agttggagag tctcaggggt gggcaccgca
      841 tggttgactg gatcggtatc gatcatcgcg aaaacgtcac tcccattcga tcgtggaagg
      901 tggcttcgac gagatgtcgt agacgacgcg gttgatcccg ttgacttcgt tgacgatgcg
      961 gttgctgatc cgggccagca cgtcgtaggg aaggcgggcc caatctgccg tcatcgcgtc
     1021 ctggcttgag atcgcgcgaa cggcgcagac gcgtccatag gtccggtaat ctcccatcac
     1081 gccggttgag cggaccggca ggaggacggc gaagtactgc cagacatcct tggcgaggcc
     1141 ggcctcctcg atttcctcac ggacgatcgc atcggcgcgg cggaggaggt cgagatcggg
     1201 ctcgttgacc gcgctcatcg tgcggaccga gagcccgggt cccgggaagg gctggcgttc
     1261 gacgatctcg tcgggaaggc cgaggctgcg accgacggca cgaacctcgt ccttgaagag
     1321 gaatttcagg ggctcgatca ggccgaactg catgtcgtcc gggagacctc cgacattgtg
     1381 gtgcgtcttg atcttggcgg cggccttcgt gtccttcgtc gtgctctcga tcacgtccgg
     1441 gtagagcgtg ccctgggcga ggaattcgaa cggaccggcc ttctcagcct ccgcctcgaa
     1501 gacgcggacg aactcgttgc cgatgatctt gcgcttctgc tcggggtcgg tgatatccgc
     1561 cagcttggtg aggaagcgct cggtcgcgtc gacgtagacg agcttcatgc cgaaggagcg
     1621 ggcgaagacc tcctgcacga gttccgcttc gccgagccgg aggagaccgt tgttgacgaa
     1681 gacgggggtc agctgatccc cgatcgcccg gtggaggagc gccgccgcta ccgacgaatc
     1741 gacgccacca ctgaggccga ggagaacgcg gccatcgccg accttctcct ggatctcgcg
     1801 aatcgcggtg tcgatgaacg attccggcga ccagtcggcg cgaatgccgg cgatatcgac
     1861 gaggaagttg cggagaatct ccgcaccctg cggcgtgtgc tgcacttccg ggtggaactg
     1921 gatgccgacg atctgcccct tcgccatcgc cgcggccgga gaattcgccg aggtcgccag
     1981 gacctcgaag tcatccggga tgcgcgtgac gtgatcgccg tggctcatcc agacattcat
     2041 gtgctcggga acattggcga agagcgggtt cttcttgccg gtcgcctcga catcgagaat
     2101 cgccgggccg tactcgcgcc gggacgccgg ggcgacttct ccaccgaggt tgtaggcgag
     2161 gagctgcatg ccgtagcaga tgccgagcac gggcagatcc tgctccagaa cccatttcgg
     2221 cagctgcggt gcgccgtcct cgtagacgct cgccggaccg ccggaaagga tcacggcctt
     2281 cggatcgagc ttcgcgacct catcccagct gacatcgtgg tggagcagct cgccgtagac
     2341 gccgacttcg cggatgcgcc gcgtgatcag ctggctgtat tgggagccaa agtcgagcac
     2401 gacgacggtg tcgacggcgg cgcggacggc actcgcttcg ccgggatcga tggaaggcga
     2461 cgctgtctcg tcgaggatgg gcgatgctgg cgtgatgtct tgcgcggtca cgggggcaga
     2521 actccatgga ttggggtgaa gggagaccgg ggtcgggatg caagcgcaac cgtggtgcgg
     2581 cggccactcg ccggcgggcg gagcgccgac ggcatcgtcg acatcaggtt tcgtcattct
     2641 acaacgttcc gctcatcgtg ccccgccggg cccgcctccc gacgcagaaa cgtcctcctg
     2701 ccgcagatac gccctcccga gcttgtcgag ggatcgcctg gcgtccacaa gctcccgccg
     2761 cgacgccatc cccgcatccg gagccaacgg cgaggccgac ccggaacggc gacaagcgcc
     2821 gtggggacgg aatggggggc gtcgatccag acatgtcttg aggcgggacg cgaacgaacg
     2881 ccgcgcaaac ggcctcgaaa cacgactccg tcgctatcta cgacgttcac gacgaccggt
     2941 gtttgtaggg cgagagttca tctctcgccg caagcccaac ctcaatgcag ggcgccttgg
     3001 gctcacgaac acgaatcggc tgcgccgacc cgaggcggcc acaagggccg cgtctacggt
     3061 tcctccaaac ggccatggcg gttcgaggca ggtgatcaac tgggactggg cggacatgtg
     3121 aagtcattcg gggtcggctg actcgtcaga cccgccgaga tagacgtatt ccttgtcgaa
     3181 ggtccagtcg tggatcgttt cgagctcgat ctcgagccgg ggatgcaacg ggtcgatctg
     3241 cttggtgaga tgcagatcga ccacggagcg gtcatcgaag ccgagattgg tcgcgaggac
     3301 gtcgagcgcg atcttcagac cgccgtcgag gtcgcgccgc atcggggtct cgaagtagaa
     3361 ggtcagatag ataccgagca gggatttctg gatcacctgt tcctgtaccg ggtcgatccg
     3421 ccccttctcg cgcatgccgg cgatcagttt cgtcgcgtca cgcttgaagg tctgggccgt
     3481 cttgctgaga acgcgacgct tcccgaccgt cacgtactgg ttgttgacgc ccggaggaag
     3541 gggtagcgtg agactgagcg tgacgggggt ggggcggttc atccgggtat cgcgagaagg
     3601 tcgcgcaact cggcccagat tgcccagacg ttgtt
//
LOCUS       DASTMD010000878         5113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_619044, whole genome shotgun sequence.
ACCESSION   DASTMD010000878 DASTMD010000000
VERSION     DASTMD010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619044"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..784)
                     /locus_tag="VFQ54_13035"
     CDS             complement(140..784)
                     /locus_tag="VFQ54_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165964.1"
                     /translation="MIGPDSAFLVVLVGSIALGSFYRATARPASDPDWIQLGALFLVI
                     AVPSLLQVTLFPALYDALRRDAHLIEHHVQWWRLVTALGVQDGGGAGALLNLASLAIL
                     LLISAPILSWRWVVGGFAVGALSGELAGLAWQHTGGGNSVGDMGICAAILVLILFDGV
                     WFLRLAALAVVIVGATILFHKDIHGAAFVSSVLAMVVVRAGPDVADRHRVAMPS"
     gene            910..2481
                     /locus_tag="VFQ54_13040"
     CDS             910..2481
                     /locus_tag="VFQ54_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEU0165965.1"
                     /translation="MDIFELIVTLVLSVVILIWLADRLSIPYPMFLMAGGLVLGVIPW
                     TPSIELNPEIVLIVFLPPVLFQAAQTTSIREFRADFSSISRLAVGLVVVSTALVAIVA
                     HWVIPGISWPAAFVLGAVVSPPDAVAATVIFQRLGAPRRIVTILEGESLINDASALVV
                     YTFAVAAVVTGSFSWSEALSRFVIVVIVGVAVGIGIGWVLGRAVSILGDPSLAAVVTL
                     IAPVGAYLLAEKLDGSGVLAVVVAGLVHGYQGSHTLSPETRVRALAIWDLITIVINGF
                     IFILIGFELGALRLVFADQSVWSLVWHGAAVVAAMLVARFLYVFLGDLLPGANKSQAF
                     AGLGMKGQFLIAWSGLRGVVSLATALAIPLTTDTGLPFDHRDEIILITAGVIVFTLFG
                     LGLPLPWLLQRLNLADDRSYDREMRLARRATSQAMSSRLQMIVAEHPRAKEALQPMLR
                     EMRVRLESNSHAVDDHRLDEIHNLLEERVGARLQMIAAARETLLELRDRGEIGDEVRR
                     EMERRLDLQELQLAG"
     gene            complement(2519..3046)
                     /locus_tag="VFQ54_13045"
     CDS             complement(2519..3046)
                     /locus_tag="VFQ54_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165966.1"
                     /translation="MQVEFASVLGEQVFLGDRPEYRRLQRLANPVHRRRLQKPDGGIW
                     TAPLWMRGGRTSSPWIEWCRAQRFRLAEPRDVWKIVPSPESRLIVVDGAASFATVIAN
                     YRHRDSTSFLPFLDFESMREDGYAGLHVTERGYLECRHPIHGIDLFHWAVSSTIWFDW
                     TFVDVVPGSTLAARP"
     gene            complement(3168..3338)
                     /locus_tag="VFQ54_13050"
     CDS             complement(3168..3338)
                     /locus_tag="VFQ54_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165967.1"
                     /translation="MPVISRRAARLARPALALLIFLATIWLAWFTRDWRILLLGSILA
                     LVQLFGKSHRRR"
     gene            complement(3620..4762)
                     /locus_tag="VFQ54_13055"
     CDS             complement(3620..4762)
                     /locus_tag="VFQ54_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165968.1"
                     /translation="MAGEIFLVQDDGQLLPLAEQPYNAERDLQRLLADYPGLISGEQI
                     NPSSPRRWLFITQELAIPGDDSGSGRWSLDHLYLDQDGIPTLIEVKRSTDTRARREVV
                     GQMLDYAANAVIYLSVEAIRARFEISCASRGQDPREIASGFLGPGAEEDTFWQAVENN
                     LKSGRIRLLFVADEILPELQRIVEFLNLQMNPAEVIAVEVRQFVGEQTRALVPRVLGQ
                     TIAVQQTKAPAAGSKTKRKWDEQSFFELLGQRGQLEESRVAEELLAWATGSGLRVWWG
                     EGPTMGSFFPMLDGATGTRWTFSVWTYGRIEIQFQYLPQENEPLRLELRRRLNEIPGI
                     DIPLESITKRPSFKLVELAREGAMEMFIQTMTWLVKTLSTESSGAT"
BASE COUNT          982 a         1609 c         1542 g          980 t
ORIGIN      
        1 cgcgaagagc cccgtcacca atctggtgac ggggcgtcgt ccgggcggac acgtagagag
       61 cttcagatcc ggcagggtgg caccgaagtc attgcattcc ggcaaggccc gaatcgggtc
      121 cagctggcct ggatcaccat caggagggca tcgccacccg gtgccggtct gccacatcag
      181 gcccggcccg aacgacgacc atcgccaaga cggaagaaac gaacgcggct ccgtggatgt
      241 ccttgtggaa gaggatcgtc gcgccgacga tgaccaccgc gagcgccgcc agtctcagga
      301 accagacgcc gtcaaagagg atgagcacca gaatcgccgc gcagatgccc atgtccccga
      361 ccgagttccc gccgccggtg tgctgccagg cgagaccggc cagttccccg gaaagcgcgc
      421 ccaccgcgaa cccgccgacc acccatcgcc acgagaggat cggcgccgaa atcaggagca
      481 ggatggccaa cgacgcgaga ttgaggagcg cacccgctcc tcctccatcc tggacgccaa
      541 gcgccgtcac cagtcgccac cactgaacgt gatgttcgat caggtgggcg tcgcgccgca
      601 aagcatcata gagggcgggg aacaacgtca cctgaagcag ggacggaacg gcgatcacga
      661 gaaaaagcgc gccgagctgt atccagtcgg gatcggaggc cggtcttgcc gtagcccggt
      721 agaacgaacc cagcgcgatc gaaccaacca gcacgacaag aaaagcgcta tcagggccga
      781 tcactgccat tctcccggtc catctgcggg accgcgaaat gcttccggaa catcgggatt
      841 cgatgccctc agcgaacaag gtacggtata accaaatggg cggacttgtt atcgtgtgca
      901 ggacttacgg tggatatctt cgaactgatt gtgacgctgg ttctttctgt cgtcattctg
      961 atctggcttg ctgaccggct ttctatcccc tatccgatgt tcctgatggc cggaggtctg
     1021 gttctcggcg tcatcccgtg gacgccgtcg atcgaactca atccggagat cgtgctgatc
     1081 gtgttcctgc cgccggtgct cttccaggcc gcgcagacga cttcgatccg tgaattccgc
     1141 gcagacttct cctcgatcag ccgtcttgcg gttggcctcg tcgtggtctc gaccgcactc
     1201 gtggctatcg tcgcgcactg ggtcatcccc gggatcagct ggccggccgc cttcgtcctg
     1261 ggagccgtcg tttctccgcc ggatgccgtg gcggccacgg tgatcttcca gcgactggga
     1321 gcgccgcggc gaatcgtcac gatcctcgaa ggcgagagtc tgatcaacga tgcgagcgcg
     1381 ctcgtcgtct acaccttcgc cgtggcagcg gtggtgacgg gcagcttttc ctggtcggag
     1441 gccctgtcga gattcgtcat cgtggtcatc gtcggcgtcg cggtggggat cggcatcggc
     1501 tgggtgttgg gccgcgcggt ctccatcctc ggcgacccat ccctggcggc tgtcgtcacc
     1561 ctgatcgcac ccgtcggtgc gtacctcctg gcagagaagc tcgacggctc gggcgtgctt
     1621 gcggtggtcg tggcgggact cgtccacggc taccagggat cccacaccct ctcgccggaa
     1681 acccgggtgc gggcactcgc gatctgggat ctgatcacga tcgtgatcaa cggattcatc
     1741 ttcattctga tcggattcga gctgggggca cttcgcctcg tcttcgccga ccagagcgtc
     1801 tggtcgctcg tctggcacgg agccgccgtc gtggccgcga tgctcgtcgc gcggttcctc
     1861 tatgtgttcc tcggcgacct cctgccgggc gccaacaaga gccaggcctt cgccggactg
     1921 ggcatgaagg gccagttcct catcgcgtgg tctggtctcc gtggagttgt ctccctggcg
     1981 acggcactcg cgatccccct gacgaccgac acgggacttc ccttcgacca tcgggacgag
     2041 atcatcctga ttaccgcggg tgtcatcgtc ttcacgctgt tcgggctcgg gttgccgttg
     2101 ccgtggctgc tccaacggct caatctcgcc gacgaccgat cgtacgatcg cgagatgcgc
     2161 ctggcccgac gggcaaccag tcaggcgatg agctcacggc tgcagatgat cgtcgcggaa
     2221 catccccggg ccaaggaagc gctgcagccg atgctgcgcg agatgcgcgt ccggctcgag
     2281 agcaattccc acgcggtcga tgaccaccgg ctggacgaaa tccacaatct gctggaggag
     2341 cgggtagggg cgcggttgca gatgattgcc gccgcccgtg agacgttgct cgaactgcgc
     2401 gatcgcgggg agatcggtga cgaggtgcgg cgggagatgg agcgacgact cgacctccag
     2461 gaattgcagc tcgcggggta gcggccagct gcttcccagc gttcttcacc gccgcgacct
     2521 acggtcgggc ggccaacgtc gagcccggaa cgacatccac gaacgtccag tcgaaccaga
     2581 tcgtgctgga gaccgcccag tggaagagat cgattccgtg gatcggatga cggcactcga
     2641 gatagccacg ttccgtcacg tgaagaccgg cgtagccgtc ctcccgcatc gattcgaaat
     2701 cgaggaacgg caggaaggac gtcgagtccc ggtgacggta gttggcgatc accgtggcaa
     2761 aggaagcggc gccatcgacg acgatcagcc tgctctcggg gctgggaact atcttccaga
     2821 cgtctcgtgg ctccgcgagc cgaaatcgct gtgcccggca ccactcgatc cacggcgagg
     2881 aggttcttcc tcctcgcatc cacaatggcg cggtccagat tccaccgtcg ggcttctgca
     2941 accgcctccg atgcactgga tttgccagcc gctgcaagcg gcgatactcg ggcctgtcac
     3001 cgaggaagac ctgttctccg agcacggacg cgaactcgac ctgcatgtat gtcctcttcc
     3061 atgcgcactg cgttcaggcg aggtcaccat gcatcgatga gattctgaat gcgggcatct
     3121 cgttggcatc tcccggctgg ccccaagcca gcgagggaat accggaatca acgacggcga
     3181 tgtgatttcc cgaacagttg aacgagcgcg aggatgctgc cgagcaacag gatgcgccag
     3241 tcgcgggtga accacgcgag ccagatggtg gcgaggaaaa tcaggagcgc cagggcgggc
     3301 cgcgccaacc tggcggcgcg gcgcgatatc acaggcatgc gccgaactcc ttggtggccg
     3361 cgatgaccga tggaactctc cacgtcgttc gcgcccagtt cagtcgtccc actcacagcc
     3421 tacgcgaggc ctgaccgtgc ggagtgctac gatcgtgcct gcagcgcgtc acgaaccgaa
     3481 ccttgtcttc ttctttgcaa gggtgctagt accacatcag gcaaggagaa atcttcgaac
     3541 gattaggtac gtccgcacaa atcgggtatt tcacctcatg gaacagcgga atcattagca
     3601 attcgtgaat cgaccccgac tatgtggcac ctgacgactc agtcgagagc gtcttgacga
     3661 gccaggtcat cgtctggatg aacatttcca tcgccccctc gcgggcaagt tcgactaatt
     3721 tgaaactggg cctttttgtg atggactcca gaggtatatc gatcccggga atctcgttga
     3781 gccgccgccg gagttccaat cgcaacggtt cattttcctg gggtagatat tggaactgta
     3841 tttcgatgcg accatacgtc caaacgctga acgtccatcg tgttcccgtc gccccgtcga
     3901 gcatcggaaa gaatgacccc atcgtggggc cctcgcccca ccacacccgg agcccggagc
     3961 cggtggccca tgcgagaagt tcttctgcga cccttgactc ttcaagctga ccccgctggc
     4021 ccagaagttc gaagaacgat tgctcgtccc acttccgctt ggtctttgaa ccggcagcag
     4081 gtgcctttgt ctgctgcacg gcgatcgtct gtcccagcac gcgcggcaca agggccctgg
     4141 tctgttcgcc gacgaactgt ctcacttcga ccgcgatgac ctcagccggg ttcatctgca
     4201 gattcaggaa ttcgacgata cgctggagtt cgggaagaat ctcgtcggcg acgaagagaa
     4261 gcctgattcg tccagatttg aggttattct caacggcctg ccaaaacgta tcttcctcag
     4321 cgccaggccc gaggaaaccc gaagcgattt ctctgggatc ttgaccacgg ctcgcacagg
     4381 agatttcgaa ccgcgctctg atcgcttcta cgctcagata gatcacggca ttcgccgcgt
     4441 agtcgagcat ttgcccgacg acttctctgc gagcccgcgt atcggtgctt cgcttgacct
     4501 cgatgagggt agggataccg tcctgatcaa gatagaggtg gtcgagcgac caacgtcccg
     4561 aacccgagtc atctccgggg atcgccaatt cctgcgtgat gaaaagccat cgacgcggag
     4621 atgaggggtt gatctgctcg ccgctgatca gacctggata atcagcaagg agacgctgca
     4681 ggtcccgttc ggcgttgtat ggctgctcgg caagcggcaa caattgccca tcgtcctgaa
     4741 ctaggaagat ctcacccgcc acgacgttgt ctccttctca tgcgtctcga aacatatctg
     4801 attagtatgg agcccgaatc aattctaatt gatcttgcgg cgattcggcc cgaagggctc
     4861 gatcatcttc cccggtgttc agacctgcga cagccatctt cagtggatgt aaaaaccgag
     4921 actcccgctc atacgctcgc ggcgccagac accctcacga agaagccttg gcgaaatcac
     4981 gtggtttcga caacgcggca gcagacgtga gtcagtagga gtgggagtcg attgcaggcc
     5041 gcaacgcccg gtggagcccc gtcatttccc gcaccacatc gcgatccttg ggggcgggcg
     5101 tcgttcccaa tcc
//
LOCUS       DASTMD010000879         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_974631, whole genome shotgun sequence.
ACCESSION   DASTMD010000879 DASTMD010000000
VERSION     DASTMD010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974631"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFQ54_13060"
     CDS             <1..444
                     /locus_tag="VFQ54_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165969.1"
                     /translation="IGDIAAVADGHGDLLPGVAQVAMQEGTHTARNILRRLQHEPTRP
                     FHYKDKGMLATIGRNRAVADIGWVHLSGFPAWAIWAVVHIATLIGRRNRFTVMLQWMW
                     SYLTHGRGARIITEQPEVTKGPAAATTTEVRPQLPGAPSLRSRVS"
     gene            598..1560
                     /locus_tag="VFQ54_13065"
     CDS             598..1560
                     /locus_tag="VFQ54_13065"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0165970.1"
                     /translation="MSLTRRRLVGTGAGLAALGVGLSQFPAFAQSATPDAFPVATPNA
                     DGTRTIATIHGDVTVPANPTRIISVNFPSSVTLLELGVAPVGITSYLPILPPSIKKPE
                     GIPAIDDATTGDLNLELIASLKPDLIVGSDWLDPSLQQAPYADLTKIAPTALFEWKQA
                     AGNWPIEAAGCAAAIGKSTELAALKGKYDTHSASIKSTYADVIAQFTWDVISGSDANW
                     YLYGPTSSHGAVAVAAGIRLNAAAKQTDGYVEESFEQLNLLAETDVLLVQQASAEMLN
                     AQATYTALPAVKAKHAFASQYFFPSSYGISDALLDDIETALKSL"
BASE COUNT          309 a          595 c          497 g          294 t
ORIGIN      
        1 atcggagata tcgccgccgt cgcggatggg cacggcgatc tcctgcccgg tgtcgcccag
       61 gtcgcgatgc aggaagggac gcacaccgca aggaacatcc tccgtcgcct gcagcacgaa
      121 ccaacgaggc ccttccatta caaggacaag gggatgctgg cgacgatcgg tcgtaaccgc
      181 gcggtcgccg acatcggctg ggtccacctc agcgggttcc cggcctgggc gatctgggcc
      241 gtcgtccaca tcgcgaccct gattggacgg cgtaaccggt tcaccgtgat gttgcaatgg
      301 atgtggtcct atctcaccca cggtcgcggc gcccggatca tcacggaaca gccggaagtc
      361 acgaaggggc cggccgcggc caccacgacc gaggttcgac cgcagctgcc gggcgctccc
      421 agcctgcgat cgcgcgtctc ctgatcgtct cccggagaag cctgttgtcc acctgactga
      481 atcccgcccg aatcagtcga caaatcccga tcccatgagt aggatatccc cggtgagatg
      541 acagctgcat ctcccggggt ctttcgtttg tacgcatttc gtcgggagtc tcttgctatg
      601 tcgctcactc gccgccgcct cgtcggcacc ggcgccggac tcgccgcgct cggtgtcggg
      661 ctctcgcagt tccccgcctt cgcgcagtcg gccacgccgg atgcctttcc tgtcgctacg
      721 ccaaacgccg atggcacccg gacgatcgcg acgatccatg gcgatgtcac ggtaccggcg
      781 aacccgaccc ggatcatctc ggtgaacttc ccgtcctcgg tcacgctcct cgagcttggc
      841 gtggcgccgg tcgggatcac ctcctacctg cccatcctgc caccgagcat caagaagccg
      901 gaaggcatcc cggcgatcga cgacgccacg accggcgatc tcaatctcga gctgatcgcc
      961 agcctcaagc cggatctgat cgtgggcagc gactggctcg atccctccct gcagcaggcg
     1021 ccctacgccg acctcacgaa gatcgcgccg accgcgctct tcgaatggaa gcaggcagcc
     1081 gggaactggc cgatcgaggc ggcgggttgc gcggcggcga tcgggaaatc gaccgaactc
     1141 gcggcgttga aagggaagta cgatacccac tcggcgtcga tcaagtcgac gtatgccgat
     1201 gtgatcgccc agttcacctg ggacgtgatc agcggctcgg atgccaactg gtatctctac
     1261 ggcccgacct catcccacgg ggcagtcgcg gtcgccgccg gcattcgtct caacgccgcg
     1321 gcgaagcaaa ccgacggcta tgtcgaggaa tcgttcgagc aactcaacct gctcgccgag
     1381 accgacgttc tcctcgtcca gcaggcgagc gccgagatgc tgaacgcgca ggcgacctac
     1441 accgcgcttc cggccgtcaa ggcaaagcac gcattcgcct cgcagtattt cttcccctcg
     1501 agctacggca tctccgacgc cctgctcgac gacatcgaga ccgcactcaa gagcctctga
     1561 tccatcatcg cgaatgcccc gccgaagacg ttttcgggcg gggcatttcc atgcacggca
     1621 tctggaaact ccgagatccg caacatcctt cagtggggat ctttccatcc cgtctgcacc
     1681 tgcaggtgaa agcca
//
LOCUS       DASTMD010000880         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2760922, whole genome shotgun sequence.
ACCESSION   DASTMD010000880 DASTMD010000000
VERSION     DASTMD010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2760922"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFQ54_13070"
     CDS             complement(<1..700)
                     /locus_tag="VFQ54_13070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0165971.1"
                     /translation="MPPALRSQSLRTPSPRFAVSTFFAIAGFISANWIARIPAVTSKL
                     DLGTAELGTLLLFIGIGSLIAFQFVGRLIERYGSARTCMACAVVYVISLSFLALAPVP
                     AALGGVLLVYGFSFGAMDVAMNAQGVEVERTLKKPIMGSLHGFFSLGALIGAAVSGTI
                     AQIGIGLTPHFVAFSVLGLGIVFWANRGQIADAHHPAQQSEKKPRRFSLPPRALWPLG
                     AIAFCGAIGEGGMAD"
     gene            complement(925..>2055)
                     /locus_tag="VFQ54_13075"
     CDS             complement(925..>2055)
                     /locus_tag="VFQ54_13075"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0165972.1"
                     /translation="PPGAAAPKTTHPSVQLIDAFNASQTAVKVISRTDSSDYSVVLQK
                     TQAELAAGDAPALVTVPWAFINWASEGLGLIGLEDALTTDQVKTVFSNLRDEVIPLVT
                     LNGKTMGVPFAFSTPVFYANKDILDKAGVKVEDVLATWEGFIDAAKPIQESNDGLPVL
                     ALMGRWPSQGIIQSNGGRVLDDNGEFQVTAPEAIAAMQAIQDITLADLKVQGTAPEQS
                     QAFIGGSVPIYHASIASLSSIRSQVQFNFEVASFPKFGDKKRWMSCGGSFIGMFAQDP
                     EQQQAAFEFEKFALSEPGYTIWAQVGYMNATKFDIPILPGQEPAYVQLTEGINAETPW
                     PGARGGEIADVWDGFVNRIWDNDISADEGAKEALDQMNEIRG"
BASE COUNT          376 a          669 c          660 g          350 t
ORIGIN      
        1 agtccgccat cccgccttcg ccgatcgcgc cgcagaaggc gatcgcaccg agcggccaca
       61 gtgcgcgtgg cggtagcgaa aaccgcctcg gcttcttctc cgattgctgc gcgggatgat
      121 gcgcgtccgc gatctggccc ctgttcgccc agaagacgat gcccaacccg agcacactga
      181 aggcgacgaa gtgcggggtc agcccgatcc cgatctgcgc gatcgtgccg ctcaccgccg
      241 ccccgatcaa cgccccgagg ctgaagaacc cgtggagcga tcccatgatc ggcttcttca
      301 acgtccgctc gacttccacg ccctgggcgt tcatcgcgac atccatcgcg ccgaacgaga
      361 agccgtagac gaggagcacc ccgccgagcg cggccggcac cggcgcgagc gcgaggaatg
      421 aaagcgagat cacatagaca acggcgcagg ccatgcacgt ccgcgcgctg ccatagcgtt
      481 cgatcagccg gccgacgaac tggaaggcga tcagggatcc gatgccgatg aagaggagga
      541 gggttcccaa ctccgccgtt cccagatcga gcttgcttgt gacggcggga atccgcgcga
      601 tccagttcgc gctgatgaat cccgcgatcg cgaagaaggt ggaaacggca aagcgtggac
      661 tcggtgtgcg aagtgattgc gaacgaagag cgggcggcaa gatccaatcc tgtcgatgag
      721 gtcgaaacgg cgaacgtcgt atcgagagta tgactgccat cggaccatcg cggcgacccc
      781 tttgccgccc cggaaaaggc gccggccaac gcggcgcccg cccggaggtg aacgtcgtca
      841 cgactggatg agaagcgtgc gaaagggcgt ccggagtggt tccggacgcc cctgcgcttc
      901 agtcgatttg aaaggagatg atgtctagcc gcggatctcg ttcatctggt cgagtgcttc
      961 cttcgcaccc tcatccgcgc tgatgtcgtt atcccagatg cggttgacga agccgtccca
     1021 cacatcggcg atctcgccac cacgggcgcc cggccatggc gtctcggcgt tgatgccttc
     1081 cgtgagctgg acgtatgccg gctcctgacc cgggaggatg gggatgtcga acttggtggc
     1141 attcatgtag ccgacctgcg cccagatcgt gtagcccggc tccgagagcg cgaacttctc
     1201 gaactcgaag gccgcctgct gctgctccgg atcctgggcg aacatgccga tgaacgatcc
     1261 accgcaggac atccagcgct tcttgtcgcc gaacttcggg aagctcgcga cctcgaagtt
     1321 gaactggacc tggctgcgga tgctgctcag ggaggcgatg ctggcgtgat agatcggcac
     1381 cgaaccaccg atgaaggcct gcgactgctc cggggccgtg ccctggacct tgagatcggc
     1441 gagggtgatg tcctggatcg cctgcatcgc cgcgatcgct tccggcgccg tcacctggaa
     1501 ctcgccgttg tcgtcgagaa cgcgaccacc gttcgactgg atgatcccct gcgacggcca
     1561 gcggcccatc agtgcgagga ccggcagccc atcgttcgac tcctggatcg gcttcgcggc
     1621 gtcgatgaag ccctcccagg tcgcgagcac gtcctcgacc ttgacacccg ccttgtcgag
     1681 aatgtccttg ttcgcataga agaccggcgt cgagaaggcg aacggcacgc ccatcgtctt
     1741 gccgttgagc gtgacgagcg ggatgacctc atcacgaaga ttcgagaaga ccgtcttcac
     1801 ctggtcggtc gtcagcgcat cctcaagacc gatcaggccg aggccttccg acgcccagtt
     1861 gatgaacgcc cacggcacgg tgaccagggc cggcgcgtcg cccgccgcga gctcggcctg
     1921 cgtcttctgc agcacgaccg aatagtcgct gctgtcggtg cggctgatca ccttgaccgc
     1981 cgtctggctg gcattgaacg cgtcgatgag ctggacgctg ggatgggtgg tcttcggtgc
     2041 cgccgcgccc ggggg
//
LOCUS       DASTMD010000881         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_888232, whole genome shotgun sequence.
ACCESSION   DASTMD010000881 DASTMD010000000
VERSION     DASTMD010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888232"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /gene="nusB"
                     /locus_tag="VFQ54_13080"
     CDS             complement(<1..614)
                     /gene="nusB"
                     /locus_tag="VFQ54_13080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU0165973.1"
                     /translation="MSSTSTNVRRSPGGAHRGGKGKKRRPGGEHFPGGERPGATVKGA
                     GLRHQSRILAMQSLFESDLTQHDLDEILGRMATDEDEDVPPPIAEQAVRLARGVLGNL
                     EDIDPRIAVAAPAFPVDQLPSIDRNVLRIAIYEIFHDPSVPHRVAINEAVEIAKHYGG
                     PSSGKFVNGVLGTIVRGIRDPKPSAATPGVTEAGATDEPERNAES"
     gene            complement(668..2086)
                     /gene="accC"
                     /locus_tag="VFQ54_13085"
     CDS             complement(668..2086)
                     /gene="accC"
                     /locus_tag="VFQ54_13085"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU0165974.1"
                     /translation="MFRKILIANRGEIALRVLRACRDLHIPAVVAYSDVDRDSLAVRL
                     ADEAVCIGPAAAARSYNNIPAVISAALVTGCDAIHPGYGFLAENAYLAEICQQVGLTF
                     IGPKPDVIERMGNKAEARKVMKAAGVPLLPGTEGTVPNVAEAQLAATRIGYPVVIKAV
                     AGGGGRGMRVAFNEAELIRGYSVAQAEAESAFGNGDVYIEKFLQRPRHVEVQVLGDNF
                     GNVVAVGERDCSLQRRHQKVVEEAPAPNLNREVRDNLLKTAQKGARNAGYTSAGTLEF
                     LLDTDGHFYFMEMNTRIQVEHPVTEEVTRLDLVAWQIRIAAGEKLDIPSEQLRPVGHA
                     IECRITAENAAKDFAPSVGTVATYVAPGGPGVRVDSHLFGGYAIPPNYDSLLGKVIVW
                     GETREIAVARMERALRETVITGVTHTIPFHREIIADPEFQAGNVHTGFVPEFMERIGD
                     KLRTAASEEEADYPAYPYPTSS"
     gene            complement(2123..>2252)
                     /locus_tag="VFQ54_13090"
     CDS             complement(2123..>2252)
                     /locus_tag="VFQ54_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0165975.1"
                     /translation="TPLGRLGQPEDIAAAVAFLVSPDASFITGHVLSVDGGLSMTS"
BASE COUNT          373 a          718 c          758 g          403 t
ORIGIN      
        1 gactccgcgt tccgctcggg ctcgtctgtc gcgcccgctt cggtcacccc gggggttgcg
       61 gctgaaggct tcggatcgcg gatgccgcgg acaatcgtgc cgagcacccc gttgacgaac
      121 ttgccggaac tcggcccgcc atagtgcttg gcgatctcga cggcctcgtt gatcgcgacg
      181 cggtgcggga ccgatggatc gtggaagatc tcgtagatcg cgatacgcag cacgttacgg
      241 tcgatcgacg gaagctggtc gaccgggaat gccggcgcgg cgaccgcgat ccggggatcg
      301 atgtcttcca ggttgccgag gacgccgcgg gcgagccgca ccgcctgctc ggcaatagga
      361 ggcggaacat cctcgtcctc gtccgtggcc atacgcccga ggatctcgtc cagatcgtgc
      421 tgggtgagat cggactcgaa gagcgattgc atcgcgagga tgcgcgactg gtgccggaga
      481 ccggcgccct tcacggtcgc gcccggacgc tcgccacccg gaaagtgctc gccgccgggt
      541 cgtcgtttct tgcccttgcc gccacggtgg gcgccacccg gcgatcggcg gacgttggtg
      601 gatgtggacg acactggcgg ttcgttatca gacatgagtt ccccgaacat agagacgagc
      661 ttgaagacta ggagctggtt ggatagggat aggcagggta atcggcttcc tcttccgagg
      721 cagcggtccg gagcttgtcc ccgatgcgtt ccatgaactc cggcacgaat ccggtgtgga
      781 catttcccgc ctggaattcc gggtcggcga tgatctcccg gtgaaagggg atcgtgtgcg
      841 tgacgccggt aatcaccgtc tcccgcaacg cccgttccat ccgggcgacc gcgatctcgc
      901 gcgtttcccc ccagacgata accttcccga gaagggaatc atagttgggc gggatcgcgt
      961 agccaccgaa gagatgcgaa tccacgcgca cgccggggcc accgggggcg acgtaggtcg
     1021 cgaccgtgcc gacggacggc gcgaagtcct ttgccgcatt ctctgccgtg atccggcatt
     1081 cgatcgcgtg cccgaccggt cgaagctgct cggacgggat gtccagcttc tcgccggcgg
     1141 caatccggat ctgccatgcc acgagatcga gcctcgtcac ttcttccgtc accgggtgct
     1201 cgacctggat ccgtgtgttc atctccatga agtagaagtg gccgtcggta tcgagcagga
     1261 actcgagcgt tcccgcgctc gtgtacccgg cattcctggc gcccttctgc gcggtcttca
     1321 ggaggttgtc ccggacctcg cggttcagat tcggcgccgg cgcttcctcg acgaccttct
     1381 ggtggcgacg ctggagcgag caatcacgct cgcccacggc aaccacgttg ccgaagttgt
     1441 cgccgagcac ctgcacctcg acgtgacggg ggcgttgcag gaacttctcg atgtagacgt
     1501 caccgttgcc gaaggccgat tccgcctccg cctgggcgac gctgtagccg cggatcagtt
     1561 cggcctcgtt gaaggcaacg cgcatgccgc gcccgccgcc gccggcgacc gccttgatca
     1621 cgaccggata gccgatgcgc gtcgcggcga gctgggcctc ggcaacattg ggaacggtgc
     1681 cctcggtgcc gggaagcaag gggacgcccg ccgccttcat caccttgcgg gcttccgcct
     1741 tgttgcccat ccgctcgatg acgtccggct tcgggccgat gaaggtgagc ccgacctgct
     1801 ggcagatctc cgcgagatac gcgttctccg cgaggaagcc gtagccgggg tgaatggcgt
     1861 cgcagccggt gacaagagcg gcgctgatca ctgccgggat gttgttgtac gagcgtgcgg
     1921 cggcagccgg accgatgcag accgcctcgt cggcgagccg aacggccagc gaatcgcggt
     1981 cgacgtcgct gtaggcgacg acggcgggga tgtgcaggtc gcgacaggcc cggagtaccc
     2041 gcagcgcgat ctcacctcga ttggcgatca ggatctttcg aaacacccac gtgcctcccg
     2101 catagcgatg ttgaaatcag gcttatgacg tcatggaaag gccgccgtcc accgagagga
     2161 cgtgaccggt gatgaatgag gcgtctggcg aaacgagaaa tgcgactgcc gcggcgatgt
     2221 cttcaggctg gccgagacga ccgagtgggg tc
//
LOCUS       DASTMD010000882         1524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2316503, whole genome shotgun sequence.
ACCESSION   DASTMD010000882 DASTMD010000000
VERSION     DASTMD010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2316503"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..694
                     /locus_tag="VFQ54_13095"
     CDS             164..694
                     /locus_tag="VFQ54_13095"
                     /inference="COORDINATES: protein motif:HMM:NF025636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0165976.1"
                     /translation="MSLAESKPLAEVSVLDIVDEAGLNRSTYYLHYSDKESLVDEVLD
                     GVLEIATESCRLLKESAHPGRDRFDPSWQDSLFGLIDQRPGLFAQLLSDSGNSTFGAR
                     LQQINEDTLISIWTKDDPAPESTSIPTRIRARYAAAGIQGLIVQWLEGGKIESHDVIS
                     DWVWRLAVTEHDLSKG"
     gene            complement(827..>1524)
                     /locus_tag="VFQ54_13100"
     CDS             complement(827..>1524)
                     /locus_tag="VFQ54_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165977.1"
                     /translation="ARIELARVRVEWRTDVRAALKNRDEELLEELFTSPPEHAIDRLS
                     ASERRRSRRLIEQRRALDVLRSAVTTLDDSSIVSALNHVERVGARITDRATWVAIQEV
                     VERASLVEDVIAAAKATPYDVGRLAQLIPAAKAVGLDRDPRLVGEFSIERLESQLLRQ
                     AHIRRIRAAIGLDDDAAIVLAAVPDPYGVLAELDEPERDRVAAAIVARRQVDRDAVAA
                     RFEQERGHRPAFA"
BASE COUNT          286 a          507 c          481 g          250 t
ORIGIN      
        1 gcgttttcct gaatatagac accgtgtcta tattcttgct gcaacctagg ggatggccgc
       61 atgaaagtca agccatgcgc caaacggggg atctatgtcc ggaacagccc gagttgcgaa
      121 tgcccgctcg cgacaaacca gacagcgtct tcaggatgcc gtgttgtcat tggcggaatc
      181 gaagccgctc gccgaagtca gcgtgctgga catcgttgac gaggcaggac tgaaccgctc
      241 gacgtactac ctgcactatt ccgacaagga aagcctcgtc gacgaagtcc ttgatggtgt
      301 gctcgaaatc gcgaccgaga gttgccgcct gctgaaggag tccgctcatc ccggtcgcga
      361 tcggttcgac ccatcctggc aggattcgct cttcggactc atcgaccagc gaccaggact
      421 cttcgcgcaa ctcctgtccg attcggggaa ttcaacgttc ggggcacgtc tccagcagat
      481 caatgaggac acgctgatct cgatctggac gaaggatgac cccgcgcctg aatccacatc
      541 gatcccgacg cgaatacggg cgcgatacgc ggcggcggga attcaggggc tgatcgtgca
      601 atggctcgag ggaggaaaga tcgaatcaca tgacgtgata tcggactggg tgtggaggct
      661 ggcggtcacc gagcacgacc tgtcaaaggg gtgatcggag ccaggggacg cgagcaacgc
      721 cctctcctcc aacacgcgcc gggacacgaa tcggctccgc cgattccagg cgaccgcccg
      781 cgtcgccggg ccgcgagggg ggcggccggc ggctgcgacg gtcgcgtcag gcgaacgcgg
      841 gacgatgccc ccgctcctgc tcgaaccggg cggcgacggc gtcgcgatcg acctgacgcc
      901 gcgcgacgat cgccgcggcg accctgtccc gctccggttc atcgagttct gccaacacac
      961 catatggatc gggtacggcc gcgagcacga tcgccgcatc gtcgtcaaga ccgatcgccg
     1021 cccggatgcg acggatgtgg gcctgccgca ggagctggga ctcgagccgt tcgatcgaga
     1081 actcgccgac cagtcgcgga tcgcgatcga gaccaaccgc cttcgcggcc gggatcaatt
     1141 gcgcgagacg gccaacgtcg tacggtgtcg ccttcgccgc cgcgatcacg tcctcgacga
     1201 gcgacgcgcg ctcgacgacc tcctggatcg ccacccacgt cgcacgatcg gtgatccggg
     1261 cgccgacacg ctccacatga ttcaacgccg agacgatcga actgtcgtcg agggtcgtca
     1321 cggcactgcg cagaacatcg agcgcgcgcc gctgctcgat caggcggcgg cttcgccggc
     1381 gctccgaagc gctgaggcga tcgatcgcgt gctcgggcgg actcgtgaac agctcttcga
     1441 gcagttcctc gtcgcggttc ttcagcgccg ccctgacatc cgtccgccac tcgacgcgca
     1501 cccgcgccag ctcgatccgc gcct
//
LOCUS       DASTMD010000883         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_439103, whole genome shotgun sequence.
ACCESSION   DASTMD010000883 DASTMD010000000
VERSION     DASTMD010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439103"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VFQ54_13105"
     CDS             complement(<1..1341)
                     /locus_tag="VFQ54_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165978.1"
                     /translation="MIARIPPPWRIPLAVLAAILVVVIAIGVVLAVAAPTKDEPTPTA
                     DGSPPASPTDTSLTPTEKPAGSPSPEVSPTPVPGSLDAMLQMTPDRLSDGSLPLPFVA
                     SYADISGWMAQNGIAVPTSLSDPNVGAFERELSALSLPTSLATRGMEPIWEETYGFNL
                     AQVHQIMMSGQAPDSIFIMTGDFDATALRNAWAASGYQAVKVEGTTIWTLWPADSIDL
                     SAPASRPALGSLNNIVLLPDGTLIAAAKISQLQSVLKVRNGDASPLSSNGDVKRMIAS
                     QPDINAMISAVIEKGDLLATLPPPNSVAGATPDDTETDQTVPASSPQAVEDAIAAMPE
                     VKLTLIGLRRTAMSRGGAGTPGAVSAVPGTPATDVTTPVASTGDQADFVMILVFANDD
                     DARVASGIVSDRLATGRSTVTNQRFRERIANPQLTVTDPDSDAHLVVLHAHLTAG"
     gene            complement(1391..2386)
                     /locus_tag="VFQ54_13110"
     CDS             complement(1391..2386)
                     /locus_tag="VFQ54_13110"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU0165979.1"
                     /translation="MAGGFDEIKILGGNSNGALVDSICEYVGLPRGRAEVFKFSNDNT
                     FVRIGESVRQNDVFVVQSFTTPVNDSIMEMLIMIDTLKRASAGRITAVIPYYGYGRTD
                     KKDQPRVPITARLVAKLVVQSGADRVLTVDLHAGQIQGFFDIPVDEMSAMYLTAQYFV
                     EKRLTRPVVVSPDLGNTKRARNFAEILDAPLAIIEKRRIGNADRSEVLNLIGSVEGCQ
                     AVIVDDEIDTGGSITHAGEVALANGAMEIYATCIHPVLSGPAIERLKASSFKEIVATN
                     TIEISREKRFDRLTVLSVAPLLGEAIQRIHAGSSVSTAYRAAGSFQPRLSLVTPE"
     gene            complement(2395..>2879)
                     /locus_tag="VFQ54_13115"
     CDS             complement(2395..>2879)
                     /locus_tag="VFQ54_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HEU0165980.1"
                     /translation="QGRDVVERFVRGYLQRDMTAILATEEDTNPKGTLQEVMQERYGE
                     PPEYETIAAEGPDHARQFTVAVVLNEEQIGIGKGRSKREAQQAAARKALVALGVVAEQ
                     PDFSSVPDPVTFEAPVVSPGDVVAETPDDSGVATTISPVVHRSIRSRRHVRGSGKREG
                     "
BASE COUNT          492 a          935 c          929 g          523 t
ORIGIN      
        1 gccggcggtg aggtgcgcgt ggaggacgac gagatgggcg tcactgtccg gatcggtcac
       61 ggtgagttgc ggattcgcga ttcgctcgcg gaatcgctgg ttcgtcacgg tcgatctgcc
      121 ggtcgcgagc cgatccgaga caatgccgct cgccactcgc gcgtcgtcgt cattcgcgaa
      181 gacgagaatc atcacgaagt cggcctgatc cccggttgat gcgacggggg tcgtgacgtc
      241 cgtcgccggg gtccccggta cagccgatac ggctccgggt gtcccagctc cgccgcgcga
      301 catggcggta cgacggaggc cgatcagcgt cagcttcacc tccggcatcg ccgcgatcgc
      361 gtcctcgacc gcctgtggtg aagacgctgg aacggtctga tcggtttccg tgtcatccgg
      421 agtcgcgccg gcgacggaat tcggcggcgg cagagtcgcc agcaaatcgc ccttctcgat
      481 caccgccgag atcatcgcat tgatgtccgg ttgcgacgcg atcatcctct tgacgtcgcc
      541 attggacgag aggggtgacg catcgccgtt ccggaccttg agcacggatt gcagctgcga
      601 gatcttcgct gcggcgatca gggttccgtc cggcaacagc acgatgttgt tcaggctgcc
      661 cagcgcgggg cgactcgccg gcgccgagag atcgatcgaa tcggcgggcc agagcgtcca
      721 gatcgtcgtc ccctccacct tgaccgcctg atatccgctt gccgcccagg cattccgcag
      781 ggccgtggca tcgaaatcgc cggtcatgat gaagatcgag tccggagcct ggcccgacat
      841 catgatctga tgcacctggg cgagattgaa gccataggtc tcctcccaga tcggctccat
      901 gccgcgcgtc gcgagactcg tcggcaagga gagtgccgaa agttcacgct cgaaggcgcc
      961 gacattcggg tcggaaagac tcgtcggcac cgcgatgccg ttctgtgcca tccagccgct
     1021 gatatcggca tagctcgcga cgaacggcaa cgggaggctc ccgtccgaaa gccggtccgg
     1081 cgtcatctgc agcatcgcgt cgaggctgcc gggcacgggt gtcggcgaga cctccgggga
     1141 cggactcccg gcgggcttct cggtcggagt cagcgaggtg tcggtgggag aggccggcgg
     1201 cgaaccgtcc gcggtcggcg taggttcgtc cttcgtcggc gccgccacgg cgaggacgac
     1261 gccgatcgcg atgacgacga cgagaatcgc cgccagcacc gcgaggggga tgcgccatgg
     1321 aggagggatc cgggcgatca tcgccggatc ccgatctgga tctggatcag ctgtccgcgt
     1381 cgcccgatca ttactccggg gtcacgagcg agagtcgcgg ctgaaaggaa cccgccgccc
     1441 gataggccgt cgagacgctc gatccggcgt ggatccgctg gatcgcctca ccgagcagcg
     1501 gcgccaccga gaggacggtc aggcgatcga agcgcttctc gcgggaaatc tcgatcgtgt
     1561 tggtcgccac gatctccttg aacgagcttg ccttcaggcg ctcgatcgcc ggtccggaga
     1621 gcaccgggtg gatgcaggtc gcgtagatct ccatcgcccc attcgcgagg gcgacctcac
     1681 cggcgtgcgt gatcgaaccg ccggtgtcga tctcgtcatc gacgatcacg gcctgacagc
     1741 cttcgaccga gccgatcagg ttcaggacct cggacctgtc ggcattgccg atccgccgct
     1801 tctcgatgat cgcgagcggc gcatcgagaa tctccgcaaa gttccgggcc cgcttggtat
     1861 tgccgaggtc cggggatacg accaccggtc tcgtcaaccg cttctcgacg aaatactgtg
     1921 cggtgagata catcgcactc atctcatcga ccgggatatc gaagaatccc tggatctggc
     1981 ctgcgtgcag gtcgaccgtg aggacgcgat ccgcgccgga ttgaacgacg agtttcgcca
     2041 cgaggcgggc cgtgatcggc acgcgcggct ggtccttctt gtcggtccgg ccatacccgt
     2101 agtacgggat caccgcggtg atccgccccg ccgacgcccg cttcagggtg tcgatcatga
     2161 tcagcatttc catgatcgaa tcgttgaccg gcgtcgtgaa tgactggacc acgaagacat
     2221 cgttctgtcg aacgctctcg ccgatccgaa cgaaggtgtt gtcgttgctg aacttgaaga
     2281 cctcagcccg gccacgcggc agcccgacgt actcgcagat ggagtcgacc agtgcaccgt
     2341 tggaattgcc acccaggatc ttgatttcgt cgaagccgcc cgccatcggt cttgttaccc
     2401 ttcccgcttg ccggagccgc gaacgtgccg acgcgaacgt atcgatcgat gtactaccgg
     2461 ggagatcgtc gtggccactc cggagtcgtc gggcgtttcc gccacgacat ccccgggaga
     2521 aacgacggga gcctcgaacg tcaccggatc gggaacggat gaaaagtccg gctgctcggc
     2581 gacgacgcca agggccacca gggctttccg cgcagccgcc tgctgggcct cgcgcttgct
     2641 ccggcccttg ccgattccga tctgttcctc attgagaacg acggcaaccg tgaactgccg
     2701 cgcatgatcc ggcccctcgg cggcaatcgt ctcatactcg ggcggctcgc cgtagcgttc
     2761 ctgcattact tcctgcaacg tccccttcgg gttcgtgtct tcttcggtcg cgagaatcgc
     2821 cgtcatgtcc cgctggagat agccgcggac gaagcgctcg acaacatccc gaccctggt
//
LOCUS       DASTMD010000884         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2317108, whole genome shotgun sequence.
ACCESSION   DASTMD010000884 DASTMD010000000
VERSION     DASTMD010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317108"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFQ54_13120"
     CDS             complement(<1..743)
                     /locus_tag="VFQ54_13120"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0165981.1"
                     /translation="MSSNTSDQDDSRGNVRSSLRGTPLSRRDLGKYAAVAAGLAALVP
                     AVAGAQTPEASPAATPSAPSTLGTPSPDRPRGGSVTVARAGDSDSLDPAHTTAGISFQ
                     VFNNIYDTLIGFNADLTYEGILAESWDISDDGLTYTFHLRKGITFHDGTDFNADAVKF
                     TFDRLMDPKTAAPAAGWVTAIKGSTVIDPSTVTIDLSTPFSPLLGNIALSYFGIVSPT
                     AVQKYGDDFSTNPVGTGPWKFKEWVQGDH"
     gene            complement(1128..>1816)
                     /locus_tag="VFQ54_13125"
     CDS             complement(1128..>1816)
                     /locus_tag="VFQ54_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEU0165982.1"
                     /translation="KGTIMSNVTETIAQQVATMHASRADQPMDDAMRAWAAEQAQLEA
                     AGVPPTVAIPGSIFPDGDLLDVDGNPTTLTGERAARPAVVVFYRGDWCPYCNLTLRTY
                     QQQLLPVLSEREIALIAISPQKPDGSLSIQEKNDLTFTVLSDPGNQIASQLGILNPER
                     SEAVRNAAASYGLDVAGGNADGTDALPMPTTAIVDANGTLRWIDVHPNYTTRSEPETI
                     LAAIKATIDR"
BASE COUNT          303 a          513 c          643 g          357 t
ORIGIN      
        1 atgtgatcgc cctggaccca ctccttgaac ttccatggtc ctgtcccgac cgggttggtc
       61 gagaagtcgt caccgtactt ctgcaccgcg gtcggcgaga cgatgccgaa gtaggagagg
      121 gcgatattgc cgagcaacgg cgagaacggc gtgctgaggt cgatcgtcac cgtggaggga
      181 tcgatcaccg tcgatccctt gatcgctgtc acccagccgg ccgccggggc ggcggttttc
      241 ggatccatca ggcgatcgaa ggtgaacttg acggcatcgg cgttgaagtc ggtgccgtcg
      301 tggaaggtga tccccttgcg gagatggaag gtataggtca ggccgtcgtc ggagatatcc
      361 cacgattcgg cgaggatgcc ctcgtaggtc aggtccgcgt tgaagccgat cagcgtgtca
      421 tagatgttgt tgaagacctg gaaggagatg ccggcggtgg tgtgcgccgg gtcgagcgaa
      481 tcggagtcac cggcgcgggc gacggtaacc gaaccgccac gcgggcgatc cggcgacgga
      541 gtcccaaggg tcgagggagc cgacggcgtc gctgccggcg aagcctcggg tgtctgtgcc
      601 ccggccacgg ccggaacgag cgcggcaaga ccggccgcga ccgcggcgta cttcccgagg
      661 tcacgccggc tcagcggcgt gccacgcaat gatgaccgaa cgttgcctct ggaatcatcc
      721 tgatcactgg tgttcgagct catgggactc ctcttcgacg gttacgcgtt tccgctggcc
      781 tggcatgcga ttccgtacgg accggtgtgt tcggaacgtc gtcaccaagg ctatcggcag
      841 atctgcctat tgggaagatg gtaattgtga ccaaacatag ccacaaagtg cacatggatg
      901 cgcgtgtgcc ggtccgggga gggctggggc tgaacgccta ttcggagagc ggtaatgtcc
      961 acacaaacct ggatcgggac gcttgtgcag gatgcggcag ggatcccctg gcacgcgtgc
     1021 acgggtggga atctggcgcc ctcgccgatg cgtagcagaa cgccggtggg gcggctcttg
     1081 ctgctccacc ggcgttctgc gtttgggatc ggggtgctgg ctgctggcta cctgtcgatc
     1141 gttgccttga tggcggccag aatcgtctcc ggctcgctgc gtgttgtgta gttcggatgg
     1201 acgtcgatcc agcggagggt gccgttcgcg tcgacgatcg cggtggtcgg catcggcaag
     1261 gcatcggtgc catcggcgtt gccgccggcg acatcgagtc catatgacgc ggcggcgttg
     1321 cggaccgcct cggagcgctc cggattgagg atgccgagtt ggctcgcgat ctggtttccg
     1381 ggatcggaaa gcacggtgaa ggtcaggtcg ttcttctcct ggatcgagag agatccatcc
     1441 ggtttctgcg ggctgattgc gatcagtgcg atctcgcgtt cactgagaac ggggagcagt
     1501 tgttgctgat aggtgcgaag cgtgagattg cagtatgggc accagtcgcc acgatagaag
     1561 accacgaccg cgggccgggc agcccgctcc ccggtgagcg tcgtcggatt gccgtcaacg
     1621 tcgagcaggt cgccgtctgg aaagatcgac cccgggattg cgaccgtcgg aggaacaccg
     1681 gccgcctcga gctgtgcctg ctctgctgcc caggcgcgca tcgcgtcgtc catgggctga
     1741 tcggcgcgac tggcgtgcat ggtggcgact tgttgcgcga tggtctcggt gacgttggac
     1801 atgatcgttc ctttca
//
LOCUS       DASTMD010000885         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1870499, whole genome shotgun sequence.
ACCESSION   DASTMD010000885 DASTMD010000000
VERSION     DASTMD010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870499"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VFQ54_13130"
     CDS             complement(<1..868)
                     /locus_tag="VFQ54_13130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0165983.1"
                     /translation="MTSGDSATPATPQNPDSDKKHLVYAVFANQTQWLLRLATGKLDE
                     PAPDAFHVRVWRTGTLVAARTGTSGVILASADDGPASDYDLVLSWLTATGSGDVLIWS
                     AAPNDAANQALLARGAADSFAPHWMWKSLARPVPALVPPDRIELADATAGDLPELTAA
                     GNRDIPYFDEQQLRIMIRLAEEDGAGAHVRIILAREVTRIRRTRIVGLVAVNTIDRDG
                     SRTAGLFNLGVRADARGRGIGTALTLAALAAAQQQGATSIGLNATRDGERIYRKLGFR
                     SIGDGQTWFMPSA"
     gene            complement(1228..1428)
                     /locus_tag="VFQ54_13135"
     CDS             complement(1228..1428)
                     /locus_tag="VFQ54_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005716508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU0165984.1"
                     /translation="MSDSTKDRVDGAGDKVSGSIKEGVGKLAGDEQTENEGKADQAKG
                     DLKQGIADVKDKVSDAVKKITH"
     gene            1713..2036
                     /locus_tag="VFQ54_13140"
     CDS             1713..2036
                     /locus_tag="VFQ54_13140"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEU0165985.1"
                     /translation="MANHTTTNTSTSNDRGFAAMDDDKQRAIASKGGHAAHESGHAHE
                     FTTQEAREAGRKGGVAAHESGNAHEFDAEEARVAGRKGGESVSSDRQHMADIGRKGGE
                     SRQNA"
BASE COUNT          350 a          614 c          729 g          355 t
ORIGIN      
        1 gggcggaggg catgaaccag gtctgcccgt cgccgatgga acggaacccg agcttccggt
       61 agatgcgttc gccatcccgc gtcgcgttga gcccgatcga tgtcgccccc tgctgctggg
      121 cggcggcgag cgcggcgagg gtcagcgccg tcccgattcc ccgtccgcgc gcgtcggcgc
      181 gcacgccgag attgaagagt ccggcggttc gcgatccatc gcgatcgatc gtgttgacgg
      241 cgacgagccc gacgatgcgc gtgcggcgga tccgggtgac ctcgcgagcg aggatgatgc
      301 ggacatgggc gccggcgccg tcttcttcgg cgaggcgaat catgatccgg agttgctgtt
      361 catcgaaata ggggatatcg cggttgccgg cggcggtcag ttccgggagg tcgcccgcgg
      421 tcgcgtcggc gagctcgatt cgatccggag gaaccagggc aggaacggga cgggcgagcg
      481 acttccacat ccagtgggga gcgaaggaat cggcggcgcc gcgtgcgagc agggcctgat
      541 tcgctgcatc gttcggcgcg gcggaccaga tcaggacatc gccgcttccc gtcgccgtga
      601 gccagctgag caccagatcg tagtcgctgg cggggccgtc gtccgcggag gcgagaatga
      661 cgccggacgt tcccgtcctc gcggcgacga gggtgccggt ccgccagacc cggacgtgaa
      721 acgcatccgg cgccggttcg tcgagcttgc cggtggcgag gcgcagcagc cattgcgtct
      781 ggttggcgaa gacggcgtag acgaggtgct tcttgtcgct gtcggggttc tgcggagtcg
      841 cgggtgttgc gctgtcgcct gaggtcatgc ggtcgatccg gccattgtga cgttgaccaa
      901 gggttgcgag gactcctgca tccgatgcga accgcagatg gaaggccagg atcagctcgc
      961 cgggaatcga tgcgcggagg cacgaaaaaa ccagcccgcg gtcgcagagg gatggagctc
     1021 cacgaccgcc actggccgcc tcagaaggga aggctgaggt gtacgtactg tatccgtttc
     1081 tggcatctgt gtcaagatgt caccattgga atagctggaa tctggcacgg tgtcgcggaa
     1141 tgcgggcgac gcacgatcgc ggcgaccgga ccaatgtccg gtcgccgcga tgtcgattgg
     1201 cagatcctgg tcgacgggag cggtcggtca gtgggtgatc ttcttgaccg cgtcgctgac
     1261 cttgtccttg acgtcggcga tgccctgctt cagatcgccc ttggcctgat cggccttgcc
     1321 ctcgttttcg gtctgctcgt cgccggcaag cttgccgacg ccttccttga tcgacccgga
     1381 aaccttgtcc ccagctccgt caactcgatc cttcgtggaa tcgctcatga ctcacctctt
     1441 tgtgtgtggt cccgtcggac tcgtgtcacg ccgaaagccg cgtgcaggga gcagggcgca
     1501 cgggttgtgc cacgaaggac cggtttatgc gtttctgccg aatctcgagc gcgggcacgc
     1561 ggaaatcggt ggacgccggt catacccaac cgggtgaacc cgttcgtggc tcggtgccgg
     1621 atgcggaggc gtggcacgtc atctgcgaac tgcacggtgt gcggctggca ggttcgttgg
     1681 aggccttccg ctgatggaaa ggaatcgtca ggatggcgaa tcacacaacg acgaacacct
     1741 ccacctccaa cgatcgcggt ttcgccgcga tggatgacga caagcagcga gcaatcgcca
     1801 gcaagggcgg tcacgctgcg cacgaatccg ggcacgctca cgagttcacg acccaggaag
     1861 cccgggaagc cggacgcaag ggcggcgtcg ccgctcacga atccggcaac gcgcacgagt
     1921 tcgacgccga agaggcccgc gtggcgggtc gcaagggtgg cgagagcgtg agttcggatc
     1981 gtcaacacat ggcggatatt ggacggaagg gcggcgagag tcgccagaac gcgtagagcc
     2041 ggcatgat
//
LOCUS       DASTMD010000886         3631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_531474, whole genome shotgun sequence.
ACCESSION   DASTMD010000886 DASTMD010000000
VERSION     DASTMD010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531474"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFQ54_13145"
     CDS             complement(<1..423)
                     /locus_tag="VFQ54_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0165986.1"
                     /translation="MSSEFDYIVVGAGSAGCIVASRLSEDPAVTVALIEAGSPPEGRL
                     FSVPSQWSRQFTTQWDWDYNSEPEPELHDRRNYLPRGKTIGGTGAMNGMLYVRGVPQD
                     FDDWEAAGNPGWGWDDVLPIFLRSENNQRGESAFHATGG"
     gene            complement(413..1501)
                     /locus_tag="VFQ54_13150"
     CDS             complement(413..1501)
                     /locus_tag="VFQ54_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU0165987.1"
                     /translation="METARRNRVTVIAGDGIGTEVMPVAIECLDSALASVEASPISWV
                     ERDWGSDHFDRHGRMMPADGLELMLEDRAIFLGAVGRPDIPDTETLWGLLIPIRREFG
                     QYVNLRPVRTYPGVPSPVGSTEIDMVIVRENNEGEYSEIGGRLYRGDEMESAQQTTVF
                     TRRGITRVAAFAAELARSRGRLLTSATKSNGIIHTMPFWDEVVEETVAGYPDVRLEKI
                     LIDALAAKLVAKPESFDVIVASNLFGDILSDLAGALAGSIGIAPSGNIDPTREHPSMF
                     EPVHGSAPDIAGKGIANPVGQILSGVMLLEHLGEQRAADLLTAAVETTLADGVRTADL
                     GGSASTIQFGAAVLARITSPKGATSHVE"
     gene            complement(1528..3048)
                     /locus_tag="VFQ54_13155"
     CDS             complement(1528..3048)
                     /locus_tag="VFQ54_13155"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEU0165988.1"
                     /translation="MVEQIAALEIEHLSKTFAETRALKDVSMVFPRGSVTALLGQNGS
                     GKSTLVKILAGFHTPDADARVSIGGREITLPIKPSDVYQQGLRFVHQDMALVDEMTVA
                     ENFAFLQGFPSRTSLSPIPDKKYNERVRQSLAALGLDIDPTARIIDIAPSQRMMVAIA
                     RAFADGNQSNSQSSDRVVFLDEPTAFLPRDSVAHVHRLIAAIAAAGGTVVYVTHRLDE
                     VLSIADRVIVLRDGAVTAQRAVKNATAQELSDLIAGGVMPAKKSSTHTDATRGEVLVR
                     ITELAGDRVSGINLDIHRGEIVGVAGLIGSGRSELARILAGIQPLRGGSMTLDGADYS
                     PRNPREAIRRGVAFAPPDRRGQGIIAEMTFRENVTLGDLGQFFSRGLLQGRRERSTVE
                     ALMKSFGVQPPRSERLMGEFSGGNQQKGIIAKFLRLNPSLLVADEPTQGVDVLGKRDI
                     AELLREFADNGGSVLLASTDFDEVSSVCDRVIVLDRGQQVGVYEAREITEQILNRV"
     gene            complement(3085..>3631)
                     /locus_tag="VFQ54_13160"
     CDS             complement(3085..>3631)
                     /locus_tag="VFQ54_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0165989.1"
                     /translation="GKANLATFSDQENPILRGRSTGNTQYLKSCPQCYNVVQDQPFTI
                     ATINSTFPTAVKGVLASHSDVNVAWVAFDGAAAAAVPAIQQAGLQKKVKVVSFDGDKQ
                     NMQWIADGTVNVATIAVPLEWVGWAGVDQANRAFTKLDFANENIPLRLVTKENASKYT
                     KAGFTGDFDYQSAFKKLWGVN"
BASE COUNT          586 a         1198 c         1164 g          683 t
ORIGIN      
        1 accgccggtg gcgtgaaagg cggattcacc gcgctggttg ttctccgatc gcagaaagat
       61 cggaagcacg tcatcccacc cccagccggg gttgccagca gcctcccagt cgtcgaagtc
      121 ctgcgggact ccgcggacat acagcatgcc gttcatcgcg ccggtccccc cgatcgtctt
      181 tccgcgcggc aagtagttgc ggcgatcgtg gagctcgggc tcaggctcag agttgtagtc
      241 ccagtcccac tgcgtcgtga actggcgcga ccactgcgac ggaaccgaga acagccgtcc
      301 ctccggcggg ctccccgctt cgatgagggc gaccgtgaca gcggggtcct cgctcagacg
      361 gcttgcgacg atgcagcctg ccgagccggc tccgacgacg atgtagtcga attcactcga
      421 catgggatgt tgcccctttc ggtgaggtga ttctggcgag gacggcggcg ccgaattgga
      481 tggtcgatgc ggagccgccc agatccgccg tccgcacgcc atcggcgagg gtcgtctcca
      541 ccgcagcggt cagaagatcg gcggcacgct gctcgccgag atgctcgagc agcatgacgc
      601 cggacaggat ctgtccgacc gggttcgcga tgcccttgcc cgcgatatcg ggcgcggaac
      661 cgtgaacagg ttcgaacatg gacggatgct ctcgcgtcgg atcgatgttc ccgctcggcg
      721 cgatgccgat cgatccagcc agtgcgcccg ccaggtcgga gaggatgtct ccgaacaggt
      781 tcgatgcgac gatcacgtcg aacgactccg gcttcgcgac gagcttcgcc gcgagcgcgt
      841 cgatgaggat cttctccaga cgaacgtccg gatagcccgc gacggtctcc tccacgacct
      901 cgtcccagaa gggcatggtg tggatgatcc cgttcgactt cgtcgccgag gtcagaagcc
      961 gaccccgcga ccgcgcaagc tccgcggcga acgcggccac ccgcgtgatt ccccgtcggg
     1021 tgaagacggt cgtctgctgg gcgctctcca tctcatcgcc gcggtagaga cgaccgccga
     1081 tctcggagta ctctccctcg ttgttctcgc ggacgatgac catgtcgatc tccgtcgagc
     1141 cgacgggcga cggcacaccg ggataggtgc gcaccggtcg caggttcacg tactgcccga
     1201 actcgcgtct gatcgggatg agcagtcccc acagggtctc ggtgtccggg atgtccggcc
     1261 ggccgaccgc gccgaggaag atggcccggt cctctaacat gagctccaga ccgtcggcgg
     1321 gcatcatccg tccgtgccgg tcgaagtggt ctgagcccca gtcccgttcg acccaggaga
     1381 tcggcgatgc ttcgaccgag gccagagcgc tgtcgaggca ttcgatcgcc acgggcatca
     1441 cctcggtgcc gatgccgtcg cccgctatga ccgtgacccg attccggcgt gccgtttcca
     1501 ttgatgcgct ccttcgcggt gaggcggtca gaccctgttg aggatctgct cggtgatttc
     1561 gcgtgcctcg tatactccga cctgctgtcc gcgatcgagg acgatgacgc gatcgcagac
     1621 cgacgagacc tcgtcgaagt ccgtcgaggc gagcagaacg cttccgccgt tgtcggcgaa
     1681 ttcccggagg agctcggcga tatcccgctt gccgaggacg tcgactccct gggtcggttc
     1741 atccgccaca agcaacgagg ggttcagccg caggaacttc gcgatgatcc ccttctgctg
     1801 attgccaccg ctgaactccc ccatgaggcg ttcggatcgc ggcggttgaa cgccgaagct
     1861 cttcatcagg gcttccacgg tcgaacgttc gcgccggccc tgcagcaggc cgcgtgagaa
     1921 gaactgaccg agatcgccca gagtcacgtt ctctctgaac gtcatctcgg cgatgatccc
     1981 ctgaccgcgc cgatcgggag gagcgaacgc gactccccgc ctgatcgcct cgcgcgggtt
     2041 cctcggtgag tagtctgccc cgtcgagggt catggatccc cctcgaagcg gttggatgcc
     2101 tgcgaggatc cgcgccagct cgctgcgtcc gctgccgatc aggccagcca ctccgacgat
     2161 ctcgccacga tggatgtcga ggttgatgcc agagacgcgg tcccccgcga gctcggtgat
     2221 gcgcacgagg acctcgcctc tcgtcgcgtc cgtgtgcgtc gacgacttct tcgccggcat
     2281 gacgccgccc gcgatcagat ccgacagctc ctgtgctgtc gcgttcttca cggctcgctg
     2341 cgcggtgacc gcgccatccc gcagcacgat cacccggtcg gcgatcgaga gcacctcgtc
     2401 cagtcggtgc gtcacgtaga ccacggttcc gcccgccgcg gcgatcgcgg cgatgagccg
     2461 gtgcacgtgg gccacggagt cacgcggcag aaaggccgtc ggctcgtcga ggaacaccac
     2521 acggtcggaa gactggctgt tcgattgatt gccgtccgcg aacgcccggg cgatcgccac
     2581 catcatccgc tgggacgggg cgatgtcgat tatccgcgcc gtcggatcga tgtcgagtcc
     2641 gagcgccgcg agcgattgac gcacccgttc gttgtacttc ttgtccggga tgggcgaaag
     2701 gctcgttcgc gacgggaagc cttgcaggaa cgcgaagttc tccgcgacgg tcatctcgtc
     2761 gacgagcgcc atgtcctggt ggacgaatcg cagtccctgt tgatagacgt cggatggctt
     2821 gatcggaagc gtgatctccc gacccccgat gctgacgcgt gcgtcagcat cgggggtgtg
     2881 gaaaccggca aggatcttga ccagggttga cttgccgctg ccgttctgtc cgagcagtgc
     2941 ggtgacgctt cctcgcggaa acaccatcga cacgtctttc agcgcgcgag tctcggcaaa
     3001 cgtcttcgac aggtgctcga tctcgagtgc ggcgatctgc tcaaccatgg ttgccttcct
     3061 tgcgatgggt atgtcgcgcg tgggtcagtt gacgccccac agcttcttga acgcggactg
     3121 gtagtcgaag tcgcccgtga aaccggcctt cgtgtacttc gaggcgttct ccttggtcac
     3181 gagacgcagc gggatgttct cgttggcgaa gtcgagcttg gtgaacgccc ggttggcctg
     3241 gtcgacaccc gcccatccca cccattcgag cggcacggcg atggtcgcga cattcacggt
     3301 gccgtcggcg atccactgca tgttctgctt gtctccgtcg aaggagacga ccttcacctt
     3361 cttctgcagc cctgcctgct ggatcgcggg aacggctgcc gccgccgcac cgtcgaatgc
     3421 cacccaggcc acgttcacgt ccgagtggga ggcgagcacg cccttgaccg ccgtcgggaa
     3481 ggtcgagttg atcgtcgcga tcgtgaacgg ctggtcttgg acgacgttgt agcactgcgg
     3541 gcagctcttc aggtactggg tgttgcccgt gctcctgcct cgcagaatcg ggttctcctg
     3601 gtcgctgaag gtggcgagat tcgccttgcc c
//
LOCUS       DASTMD010000887         1621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_439733, whole genome shotgun sequence.
ACCESSION   DASTMD010000887 DASTMD010000000
VERSION     DASTMD010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439733"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VFQ54_13165"
     CDS             <1..624
                     /locus_tag="VFQ54_13165"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEU0165990.1"
                     /translation="CAYTEARSIERGESTIAHDSRRIQRIFLEPQAPEAYEPALQAII
                     NADLIVLGPGSLYTSVLPNLLVDGITEAIKWSRGTTAYVCNVATQHGETDHFGYEDHI
                     REVIAYLGKGELDCAIVNDNPAAFGAINEINDVEAIVYDGADSVADGVKIYARDVVSD
                     PNPLRHDPAKLATILLEIARESRAASIQSPRPVIEDPTRATPVNVAA"
     gene            860..>1621
                     /locus_tag="VFQ54_13170"
     CDS             860..>1621
                     /locus_tag="VFQ54_13170"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU0165991.1"
                     /translation="MTFKVGINGFGRIGRQVFKAIDEGGFSDLFEVVAVNDLTDNKTL
                     AHLLKYDSTYGQYRAEITHDDEAISVNGKSVKVLAEREPAKLPWKDLGVDLVIESTGR
                     FTDADQAKAHIDAGARKVIISAPAKGEDITIVLGVNEESYDPASHHIISNASCTTNCL
                     APVAKVVLDNFGIKHGLMTTVHSYTADQVLQDAPHKDLRRARAATQNIIPTTTGAAKA
                     VALVIPELKGKFDGFSLRVPTPTVSIIDFVAVTEKP"
BASE COUNT          346 a          555 c          440 g          280 t
ORIGIN      
        1 tgtgcatata cggaagctag aagtatcgaa cgtggcgaat ctacaatcgc ccacgactcc
       61 cgtcgcatcc agcggatctt cctcgagccg caagcgccgg aagcctatga gcccgccctc
      121 caggccatca tcaacgccga cctgatcgtc ctcggcccgg gcagcctcta tacgagcgtc
      181 ctccccaacc tgctcgtcga cggcatcacg gaggcgatca aatggtcccg cgggacaacc
      241 gcctacgtct gcaacgtcgc aacccagcat ggcgagaccg atcacttcgg ctacgaagac
      301 cacatccgcg aggtcatcgc ctatcttgga aagggcgagc tcgactgtgc gatcgtgaat
      361 gacaaccccg ctgccttcgg cgcgatcaac gagatcaacg acgtcgaggc gatcgtctac
      421 gacggcgccg actccgtcgc cgacggcgtg aagatctacg cgcgggacgt ggtcagcgat
      481 cccaacccac tccgtcacga ccccgccaag ctcgcgacga tcctgctcga gatcgcccgc
      541 gagagccgcg ccgcctcgat ccagtcaccg cgcccggtga tcgaggatcc gacccgcgca
      601 accccggtca acgtcgccgc atagacgaag aatccggtct ggtgaagcag cagcacacca
      661 aactacactg gttacgggaa agacatcggg catgattccc gaaaacggca gagtctttcg
      721 gcattttgga caaccgccca atggaccgtt ccggtttttg gtacgcttgt ggcggctgaa
      781 gtcactgtgg atcaggccga ccacctgacc gggatgttcc tcgtatagaa cgacatccgt
      841 tatttctgga gatgaccgaa tgacattcaa ggttggcatc aatggcttcg gccgcattgg
      901 tcgtcaggtc ttcaaggcga tcgacgaagg cggcttctcc gatctcttcg aagtcgttgc
      961 cgtcaacgac cttaccgaca acaagaccct cgctcatctc ctcaaatacg actctacgta
     1021 cggtcaatac cgcgcggaaa tcacccatga tgacgaggcg atttcggtca atggcaagag
     1081 cgtcaaggtc ctcgccgaac gcgagccggc caagctgcca tggaaggacc tcggcgtcga
     1141 tctcgtgatc gaatcgaccg gtcggttcac cgatgccgac caggcgaagg cgcacatcga
     1201 tgccggcgcc cgcaaggtga tcatctccgc ccccgccaag ggtgaggaca tcacgatcgt
     1261 gctcggcgtg aacgaggagt cctacgatcc ggcgtcgcat cacatcatct ccaacgcctc
     1321 ctgcacgacg aactgcctcg ccccggtcgc gaaggtcgtt ctcgacaact tcggcatcaa
     1381 gcacgggctg atgacgacgg tccactccta taccgccgac caggtgctgc aggacgctcc
     1441 ccacaaggat ctccgtcgtg cccgcgccgc cacccagaac atcatcccca ccactaccgg
     1501 cgccgcgaag gcggtcgcgc tcgtgattcc ggaactcaaa ggcaagttcg acgggttctc
     1561 gctccgcgtc ccgaccccga cggtctcgat catcgacttc gtcgccgtca ccgagaagcc
     1621 g
//
LOCUS       DASTMD010000888         1807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_83417, whole genome shotgun sequence.
ACCESSION   DASTMD010000888 DASTMD010000000
VERSION     DASTMD010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83417"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VFQ54_13175"
     CDS             complement(<1..1074)
                     /locus_tag="VFQ54_13175"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEU0165992.1"
                     /translation="MTTSADAVPFDDTPTVLGEIGLFAGGFVSNYFHQFYDTSKFPEG
                     NRPELRRVSPEYGIRYGYNFTPNWGFELEGSLITATTKGMTIGETGPDGSAKMWNGRA
                     QTVFQLPLGGGVVPFAAVGFGVMHTTSDLLGSDTDVPLHVGGGVRFWITDQLAVRVDG
                     RYLRGPSEQAPYTLNAGYGEFGVGLAYSPKIRPERPEPVKPDGDRDGDGIRDSVDKCP
                     DQPEDKDMFQDDDGCPDPDNDGDGIPDELDKCPLDPEDKDGYQDEDGCPDLDNDGDGI
                     PDTKDKCPNEPEDKDGFQDLDGCPDLDNDKDGIPDDKDKCPDQPETINGFQDEDGCPD
                     RGDALVVLSPDRLELLESVKYDRK"
     gene            complement(1155..>1807)
                     /locus_tag="VFQ54_13180"
     CDS             complement(1155..>1807)
                     /locus_tag="VFQ54_13180"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEU0165993.1"
                     /translation="DADGCVNGLRLNYPETVKPMLEIFGLLPQCKVAVGMYMMVMQNA
                     VKFFGDTVFNIDPSADELANITVQMADAVHGFGVTPRVAMISYSNFGTVRSAGVKKIA
                     EALAIVRRERPDLEIDGEMQPEMALSSERRRQYYPFSKLTKSANTLIFPYLDPANASY
                     QVLKELGGASAVGPILLGLGKPCAALQNDASVDDIVNMTAYVVLKAQQSHAVSLRA"
BASE COUNT          300 a          595 c          577 g          335 t
ORIGIN      
        1 cttgcgatcg tatttgacgg actcgaggag ctcgaggcga tccggcgaga gcacgacgag
       61 cgcgtcaccg cgatccgggc agccgtcctc gtcctggaag ccgttgatcg tctccggctg
      121 atcgggacac ttgtccttgt cgtcggggat gccgtccttg tcgttatcga gatcgggaca
      181 gccatcgaga tcctggaacc cgtccttgtc ctcgggctcg ttcggacatt tgtccttcgt
      241 atccggaatc ccgtcgccat cgttatcgag gtccgggcag ccgtcctcgt cctggtagcc
      301 gtctttgtct tccggatcga gcgggcactt atccagctcg tcggggatcc cgtcgccgtc
      361 gttgtccgga tcaggacagc cgtcgtcgtc ctggaacatg tccttgtctt cgggctggtc
      421 gggacacttg tcgacggaat cgcggatgcc gtcgccatcg cgatcgccat ccggcttcac
      481 cggttcggga cgctcgggcc ggatcttcgg gctgtacgcg aggccgacgc cgaactcgcc
      541 gtagcccgca ttcagtgtgt acggcgcctg ctccgagggt ccgcgcagat agcgcccgtc
      601 gacccgcacc gcgagctgat cggtgatcca gaaccgcacg ccaccgccga cgtgaagcgg
      661 gacgtcggta tcgctgccga gcaggtccga ggtggtgtgc atgacaccga agccgaccgc
      721 cgcgaacggc accacgccgc cgccgagcgg aagctggaac accgtctgcg cccggccatt
      781 ccacatcttc gccgagccat cgggaccggt ctcgccgatc gtcatgccct tcgtcgtcgc
      841 ggtgatcagc gagccctcga gctcgaagcc ccagttcggc gtgaagttgt aaccgtagcg
      901 aatgccgtac tcgggcgaga cgcgacggag ctccgggcga ttcccctcgg ggaacttcga
      961 ggtgtcgtag aactggtgga agtagttcga gacgaacccg ccggcgaaca ggccgatctc
     1021 gccgaggacg gtcggcgtgt cgtcgaacgg aacagcgtcg gcgctcgtgg tcacagcgcc
     1081 gtcatcggcg accgcaagcc tggatgcggc gactaccagc gcacagacga ttccccgcaa
     1141 gtcattacat tgtatcaggc ccgcaacgag accgcgtggg actgttgcgc cttcagcacg
     1201 acatacgcgg tcatgttgac gatatcgtcg acggacgcat cgttctggag cgccgcgcac
     1261 ggcttgccga ggccgaggag gatcgggccg accgccgacg caccaccgag ctccttcagg
     1321 acctggtacg acgcgttcgc cggatcgagg tacgggaaga tcagcgtgtt cgcggatttg
     1381 gtgagcttcg agaacgggta gtactggcgc cgccgctcgg agctgagcgc catctccggc
     1441 tgcatctcgc catcgatctc gaggtccggc cgttcccggc ggacgatcgc gagcgcctcg
     1501 gcgatcttct tcacgcccgc gctccggacc gtgccgaagt tcgaatacga gatcatcgcc
     1561 acccgcggcg tgacgccgaa cccgtggacc gcgtccgcca tctggaccgt gatgttcgcg
     1621 agctcgtcgg cgctgggatc gatgttgaac accgtgtcgc cgaagaactt caccgcgttc
     1681 tgcatcacca tcatgtacat gccgaccgcg accttgcatt gcggcagcag gccgaagatc
     1741 tcgagcatcg gcttcacggt ctcgggatag ttgagccgga ggccgttgac gcaaccatct
     1801 gcatcgc
//
LOCUS       DASTMD010000889         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1333567, whole genome shotgun sequence.
ACCESSION   DASTMD010000889 DASTMD010000000
VERSION     DASTMD010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333567"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VFQ54_13185"
     CDS             <1..999
                     /locus_tag="VFQ54_13185"
                     /inference="COORDINATES: protein motif:HMM:NF027305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HEU0165994.1"
                     /translation="ADIAMAEAERDIAIRRAQALRESKIAEAAAEQEKVSAESLSQTK
                     QAEAVRDLELKRAEFDRSVQEQRAQADKAYEIQTNVQQQRVVEEQVRVERVRKEGEIA
                     VQEAEILRREKELIATVLKAADVERQRIETLAQADKQKEIFRATAQAEATRLEGEARA
                     NIVRVQGLAEAEIIQAKGQAEANAMQIRAAAYQEYNQAAVLDKLLSELPEVVRAAAEP
                     LNKVDKITIVSTGGDSGSGVGVNRISSDLTALVAQVPILIGSLTGVKMQDLLQHVPSA
                     GNQYSPRDEKVVPARKPAQTPPSAPVVEAEPVAPAPAPGVNGATAPTPEEPATAQS"
     gene            1168..>1629
                     /locus_tag="VFQ54_13190"
     CDS             1168..>1629
                     /locus_tag="VFQ54_13190"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEU0165995.1"
                     /translation="MADDFLSSRPGGDADRVRVDALGEPGQRRFRMLASVAGETHIIW
                     MEKQQMQALGLAIEQMLEQLPDRAETFEVPTLPIEFDDDTRSQFRVGRMELGFDLATD
                     RIIIGTHDVQQEESEQQPSLSVRITRRQVIELSTDAASVVAAGRPRCPMCGD"
BASE COUNT          325 a          499 c          560 g          245 t
ORIGIN      
        1 gccgatatcg cgatggcgga ggccgagcgc gacatcgcga tccggcgggc gcaggcgctg
       61 cgtgagtcga agatcgccga ggcggcagcg gagcaggaaa aggtctccgc tgaatcactc
      121 agccagacga agcaggcgga agccgtccgc gatctcgagc tgaagcgcgc cgagttcgat
      181 cgttccgtcc aggagcagcg agcgcaggcc gacaaggcgt acgagatcca gacgaatgtc
      241 cagcagcagc gcgtcgtcga ggagcaggtt cgcgtcgagc gggtccgcaa ggaaggcgag
      301 atcgcggtcc aggaggcgga aatcctccgg cgcgagaagg aactgatcgc gactgtgctc
      361 aaggcggcgg acgtcgagcg acagcgcatc gagacgttgg cacaggcaga caagcagaag
      421 gaaatcttcc gcgcgaccgc gcaggcggaa gcgacgcggc tcgaaggcga agcgcgggcg
      481 aacatcgtcc gcgtccaggg tctcgccgaa gccgagatca tccaggcgaa gggtcaggcg
      541 gaagcgaacg cgatgcagat tcgcgccgct gcctatcagg aatacaatca ggccgcggtg
      601 ctcgacaagc tcctgagcga gttgccggaa gtcgtccgcg cggcggcgga gccgctgaac
      661 aaggtcgaca agatcacgat cgtttcgacc ggcggcgaca gcggcagcgg ggttggcgtc
      721 aaccggatct ccagcgatct gaccgcgttg gtggcccagg ttccgatcct gatcggatcg
      781 ctgacagggg tcaagatgca ggatctcctc cagcacgtgc cgagcgcggg gaatcagtac
      841 tcgccgcgtg acgagaaggt cgttcccgcg cggaaaccgg cgcagacgcc gccgtcggct
      901 cccgtcgtcg aggccgagcc ggtcgctccg gccccggccc ccggcgtcaa cggcgcgacc
      961 gcaccgacgc cggaggaacc ggccacggcg cagtcgtaag cagacatctc aactgactgt
     1021 ggcggccacg gtccctcggc tcccgtaacg gagttggggg accgttggtg gtattcttga
     1081 gtggcgattt tgcagaaatc gacgaagaga tgtgtttgcg atcgctccaa tactggttgc
     1141 cagttcgctc aatgaggccc gatatccatg gctgacgatt tcctttcatc acgacccggt
     1201 ggcgacgccg atcgcgtgcg cgtcgatgcg cttggcgaac cgggccaacg ccgctttcgc
     1261 atgttggcct cggttgccgg agaaacccac atcatctgga tggagaagca acagatgcag
     1321 gcgctcggcc ttgcgatcga gcagatgctc gagcagctgc cggaccgcgc cgagaccttc
     1381 gaggttccca cgcttccgat cgaattcgat gacgatacac ggagtcagtt ccgcgtcggt
     1441 cgcatggaac tcggattcga tctcgcgacg gatcgcatca tcatcggcac gcatgacgtg
     1501 cagcaggaag aatccgagca gcagccatcg ctcagcgtcc gcatcacgcg gcgacaggtg
     1561 atcgaactga gcacggacgc cgcgtccgtg gtggctgccg gccggccgcg ttgcccgatg
     1621 tgcggtgac
//
LOCUS       DASTMD010000890         1732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_797436, whole genome shotgun sequence.
ACCESSION   DASTMD010000890 DASTMD010000000
VERSION     DASTMD010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797436"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VFQ54_13195"
     CDS             <1..1073
                     /locus_tag="VFQ54_13195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SfnB family sulfur acquisition oxidoreductase"
                     /protein_id="HEU0165996.1"
                     /translation="LDELDQFSESGLWGITIPKVNGGAGVSQATVAKVFSILAAADPS
                     LTQLAQNHFEIVDVIRVTASPEQKIELFGAILGGHRLGNAFSESGGKNVEDFSTRLVR
                     DGDAYRINGQKFYSTGALFAHLVPIVALDETGHVVVAIADRDSPGLTVVDDWSGIGQR
                     TTASGTVTIENVFIPASRVLPAHIVYERPSAAGPQSQLIHAAIDLGIAEGAVEASIDF
                     IKHRARPWIDSGLDKASDDPFTLRDIGDLKIRLHAAEALLERAGHQLDATLAETNEAT
                     IALAKVAVAEAKVLTTEVAILAANKLIELGGSRATLAQNGFDRYWRDARTHTVHDPVR
                     WKYFAVGQYYLNGVNPPLHSWI"
     gene            1078..>1732
                     /locus_tag="VFQ54_13200"
     CDS             1078..>1732
                     /locus_tag="VFQ54_13200"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165997.1"
                     /translation="MTKLAEIRRSLEEVEALVRDRGVRFLRVELPDLHGIARSKTVPI
                     KRLQAIAESGLNFPLPPLALDVQCDAVEGTGYLEERGYPDVTLELDLSTFAVLPYAEG
                     TARVISDPHHPTTGEPVAAASRTVARKAFNEAAALGFEVLSGFEYEFYLLDPSTLEPA
                     SATVRQFASFEETDRKLLFEIAEALDTLGIEVTTLNLEYGPSQFELNFAPSWGLRAAD
                     "
BASE COUNT          335 a          550 c          504 g          343 t
ORIGIN      
        1 cactcgatga actcgaccag ttctcggaga gcggcctctg gggcatcacg atcccgaagg
       61 tcaatggcgg agcgggggtg tctcaggcga cggtcgctaa agtcttttcg atcctcgcgg
      121 cggcggatcc ctcgctgact cagctcgcgc aaaatcattt cgagatcgtg gacgtcattc
      181 gcgtcaccgc gagtccggaa cagaaaattg agctgttcgg cgccattctg ggcggtcaca
      241 ggctcggcaa cgccttctct gaatcgggtg gaaagaacgt cgaggacttc tcgactcggt
      301 tggtccgcga tggcgatgcc taccgcatca acggacagaa attctattcg accggggcgc
      361 tcttcgcgca cctcgttccc atcgttgccc tcgacgagac cggccatgtc gtggtcgcca
      421 ttgccgaccg cgacagcccg ggactgactg tcgtggacga ctggtctgga attggccagc
      481 gcacgaccgc aagcggtacc gtcacgatcg agaacgtctt cattcctgct tcgagagttc
      541 ttcctgcgca cattgtttac gaaagaccga gcgcggcagg cccgcaaagc cagcttatcc
      601 acgccgcgat cgatctcggc atcgccgaag gtgcagtcga ggcgagcatc gactttatca
      661 agcaccgcgc aaggccgtgg atcgacagtg ggctggacaa ggcgagcgat gatccgttca
      721 ccctccgtga catcggcgac ctcaagatcc gtctgcatgc tgctgaggct ctcctcgagc
      781 gtgctggcca tcagctcgat gccacactgg ccgagaccaa cgaagcaacc attgcgcttg
      841 ccaaggtcgc ggtcgccgag gcgaaagtat tgaccaccga agtggcaatc cttgccgcca
      901 acaaactgat cgagctcggc ggatcacgcg ccacgctcgc tcagaacggc ttcgaccgct
      961 attggcgcga cgcccgcacg cacaccgtcc acgatcccgt gcgctggaag tatttcgccg
     1021 tcggccaata ctatctcaac ggcgtcaacc cgccgttgca ctcgtggatt tgatcccgtg
     1081 acgaagttgg ccgagatcag gcgatctctt gaagaggtgg aagccttggt ccgggatcgg
     1141 ggcgttcgat ttctccgcgt cgaactgccc gacctgcatg gcatcgcccg gtcgaagacg
     1201 gtgcccatca agcgtctgca ggccattgct gaaagcgggc tcaactttcc ccttccgccg
     1261 cttgcgctcg atgtccagtg cgacgcagtg gaaggcaccg gctatcttga ggaacgggga
     1321 tatcccgacg tcacccttga acttgatctg agcaccttcg ccgtccttcc ttatgcggaa
     1381 ggaaccgccc gcgtcatctc cgatcctcat catccgacga cgggcgagcc ggtggctgcg
     1441 gcgtcgcgga cggtcgcgcg caaagcgttc aatgaggcgg cggcgctcgg cttcgaagtc
     1501 ctgagtggct tcgaatacga attctatctt ctcgatccgt ctaccctcga gccggcatcg
     1561 gctacggtcc gccagttcgc ctcctttgag gagaccgacc gaaaactcct ctttgagatc
     1621 gccgaggcgt tagatacgct tggcatcgaa gtcacaaccc tcaatctgga atatggtcca
     1681 agccagttcg agctgaactt tgcacccagc tggggattgc gcgccgcgga cc
//
LOCUS       DASTMD010000891         1936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3118991, whole genome shotgun sequence.
ACCESSION   DASTMD010000891 DASTMD010000000
VERSION     DASTMD010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3118991"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFQ54_13205"
     CDS             <1..205
                     /locus_tag="VFQ54_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0165998.1"
                     /translation="AQPRKPREPRPQRQAASAEPAFENEDLSAMALAFAMAGGEDLLT
                     DEPEAAPKRAPRREKSEEEDKDQ"
     gene            473..>1936
                     /locus_tag="VFQ54_13210"
     CDS             473..>1936
                     /locus_tag="VFQ54_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEU0165999.1"
                     /translation="MAEKFDKFTDRARKVLTLAQEEAQRFNHNYIGTEHLLLGLVREG
                     DGVAAKVLANMGVQLPKVRSAVEFIIGRGDSMIMGEIGLTPRAKKVIELSVDEARRLN
                     HHYIGTEHLLLGLVREGEGIAAGVLESLGVNLEKVRTQVMQVVSQSASQQAQTKQQTK
                     TPYMDALGFDLTEAARTDKLGPLIGRSTEIDRVMQILSRRTKNNPALIGEPGVGKTAI
                     VEGLAQRIVAGDVPEQLQGKRLVALDIGALVAGTKYRGEFEERLKKIVNEVKETKSIL
                     FIDELHTLVGAGAAEGAVDAANILKPALSRGELQTIGATTLDEYRKYIERDAALERRF
                     QPVQVSEPSIDETIQILQGVRSLYEEHHRLKISDDALSAAANLAARYVTDRFMPDKAI
                     DLIDEASSRVRMQRSVSPPSLKEAMAGLQSLQRELESAVNGREFELAAELRDRERKLR
                     DRIDRQEQGLKDQQASEDIYVTEEDVAEVVSMWTGIPL"
BASE COUNT          403 a          600 c          615 g          318 t
ORIGIN      
        1 cgcgcagccg cgcaagccgc gtgagccccg accgcagcgc caggcggcat ctgccgagcc
       61 ggcgttcgag aacgaggatc tctcggcgat ggcgctggcg ttcgcaatgg ccggtggtga
      121 ggacctcctc accgacgagc cggaagcggc tccgaagcgt gctcctcgcc gtgaaaagag
      181 cgaggaagaa gacaaggacc agtagaaccc tggtcatttg tccgggacgc gactcccgaa
      241 atgtgataga aaagcgaaca acggcaggtg ctccgtcacc tgccgttgtt ggccatcttc
      301 cagaagccct gcacccggtc gacggtcagg taacgacatc gctcgcgata cgttgcacaa
      361 tggaggtgat ggacgtccgc gtgcacactc cttgcgtata ttcgggataa cgtcgcgcgt
      421 gcatgatgag gctgagaagt ctcgcatcca ctgaaaccgg gggagcccca ggatggcgga
      481 aaagttcgac aagtttacgg atcgagctcg caaggtcctc acgctcgccc aggaagaggc
      541 tcagcggttc aatcacaatt acattgggac tgaacatctg ctcctcgggc tagtgcgaga
      601 aggcgatggc gtcgcggcaa aggtgctggc gaacatgggc gttcagctgc ccaaggttcg
      661 ctccgccgtc gaattcatca ttggtcgtgg cgattcgatg atcatgggtg agattggtct
      721 cacaccccga gcaaagaagg tcatcgagct ttcggtcgac gaggctcgcc gcctgaatca
      781 ccactacatt ggaacagagc atcttctgct cggtctcgtt cgcgaaggcg agggcattgc
      841 cgcgggcgtg ctcgaaagcc tgggcgtcaa cctcgagaag gttcggaccc aggtgatgca
      901 ggtcgtgagc cagagcgcca gccagcaggc ccagaccaag caacaaacca agactccata
      961 catggatgcc ctcggattcg atctcaccga ggccgcccgg accgacaagc tcggtcccct
     1021 catcggtcgt tccaccgaga tcgaccgtgt catgcagatc ctctcccgcc gcaccaagaa
     1081 caaccccgcc ctcatcggtg agcctggtgt cggcaagacc gcaatcgtcg aaggcctcgc
     1141 ccagcgcatc gtcgccggcg atgtgccgga gcaattgcag ggcaagcggc tcgtggcgct
     1201 cgacatcggg gccctcgtcg ccgggacgaa gtaccgcggc gaattcgagg agcgcctgaa
     1261 gaagatcgtc aacgaggtca aggagaccaa gagcatcctc ttcatcgacg agctgcacac
     1321 actcgttggc gcaggtgccg ccgaaggcgc cgtcgatgcg gcgaacatcc tgaagccggc
     1381 actctcccgt ggcgaattgc agacgatcgg ggcgacgacg ctcgacgagt accgcaagta
     1441 catcgagcgt gacgccgctc tcgagcgacg cttccagccg gttcaggttt cggagccgtc
     1501 gatcgacgag acgatccaga tcctccaggg cgttcgctcg ctctacgagg agcatcaccg
     1561 gctcaagatc agcgacgacg cactgtcggc ggcggcgaac ctcgccgctc ggtatgtgac
     1621 cgatcgcttc atgccggaca aggcgatcga cctgatcgac gaggcgtcat cccgcgtgcg
     1681 gatgcagcgc tccgtctcgc cgccgagcct caaggaggcc atggcgggtc tgcagtcgct
     1741 ccagcgcgag ctggaatccg cggtgaacgg gcgtgagttc gagctggcgg ccgagctccg
     1801 cgatcgcgag cgcaagctgc gcgaccggat cgaccggcag gagcagggac tcaaggacca
     1861 gcaggcctct gaagacatct acgtgactga agaggatgtt gccgaagtcg tctcgatgtg
     1921 gacgggcatt ccgctc
//
LOCUS       DASTMD010000892         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2494431, whole genome shotgun sequence.
ACCESSION   DASTMD010000892 DASTMD010000000
VERSION     DASTMD010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2494431"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      340..487
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            705..1652
                     /locus_tag="VFQ54_13215"
     CDS             705..1652
                     /locus_tag="VFQ54_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0166000.1"
                     /translation="MLNRLFQRLLILVVTVIATSSAIFVLIHAAGDPTQGFLPPGSSP
                     EVRAQVRDQLGLDDPILQQYVTFVGRGLRGDFGDSWRNHQPALDAVLDRLPATLLLAA
                     SAVLLSVVIGTVLAFLGVSARLRPFRRLIGVFGLLGQAVPTFRLGTLLILIFAVKLNW
                     MPSSGNAEAKSLVLPALTLAAYPSSMIARLLQASLLEASAQTYARTARAKGLREPIVW
                     IRHALPNALLPTLAFIGVQLGFLVGGTVVVESVFAYPGVGRLALQSASERDLPVVHAF
                     AVMVAVAIVLIGTAIDLIVAIIDPRQRERDRMEGAIGIG"
     gene            1645..>2274
                     /locus_tag="VFQ54_13220"
     CDS             1645..>2274
                     /locus_tag="VFQ54_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0166001.1"
                     /translation="MADLSVLPVPLERASALQRRSSTDHESPHERRRRAWRLSSLWML
                     APVALFLLLAFGGPYLVTASPTRQDLLARLAHPFFDGGSWSHPFGTDSLGRDILARIA
                     QGARYSLYVGLIAASGTAVIGITLGMLAGAGGRLADRVVTLLSDVVMAVPFVVIGIVM
                     TAILGQSLKNVLAILIVSGWVSYARIIRLQTRFLVGSEYVLASLAMGASR"
BASE COUNT          324 a          735 c          734 g          481 t
ORIGIN      
        1 ttgcgcggga cccgcttcgc ggctcgccgt ccccaaacct gggatgacga atgaaccggg
       61 tccccggacc tgggaacggg cgaccacgag ggccgtcctt acgaaggtcg gtaagtccgt
      121 aggggcgacc ctcgtggtcg ccctatgcaa catcccattg acgatgcctt cgggcgacgg
      181 gtgcgcttcg cgcggaggtc cttccccgcg gtcacgatgg cggtgaggtt gggttcgtcg
      241 tggacattcg cggttcgacg ccggtctcgt gatcgggtgc tgacagggca attgtgaaat
      301 gattgacgta tcgacttact gatgtatagt tggttgcaac atagccttcg gggattggtg
      361 agagtgtcga gttatggcac ccaagtcctg accggcggta tagcccgcaa gcgcgagtgg
      421 gagcacgagc gcacgatccg gtgaaactcc ggggccgacg gtatagtccg gatgggagaa
      481 ggtatccgtg catctcgggc gaagcccgtg gtgtatgccg ctccgcaaca tgtcgaaccc
      541 gagggcctcc gtccccgggt atttctgtgc ccgggatcgt ttgcacggag gcgcttggtg
      601 aggcatgagg gagctggcga cggagtcgat ggcaggcgtt agcgtctgcg gttgacctgt
      661 cgtcggcgtc tcctcatctt caacaggtgc ccggcagggc acgtatgctc aatcgactct
      721 tccagcgcct gctcattctc gtggtgacgg tgatcgcgac ttcgtccgcg atcttcgtgc
      781 tgatccatgc cgcgggcgat ccaacccagg gcttcctccc accgggatcc tcccccgagg
      841 tccgcgccca ggtgcgcgat cagcttggtc tcgacgatcc catcctccag caatacgtga
      901 ccttcgtcgg tcgcggtcta cgtggagatt tcggcgattc gtggcggaat caccagccgg
      961 cgctcgatgc cgtgctcgat cggctccccg cgacgctcct gcttgcggca tccgcagtcc
     1021 tgctttcggt cgtgatcggc accgttctcg cattcctcgg cgtctctgcg cgactgcgtc
     1081 cgttccggcg tctgatcggg gtcttcggac tcctcgggca ggcggtgccg accttcaggc
     1141 tcgggacgct gctgatcctg atcttcgccg tgaagctgaa ctggatgccg tcgtccggca
     1201 acgccgaggc gaagtccctc gtgctcccgg cgctcacgct cgcggcgtat ccgtcctcga
     1261 tgatcgcgcg gcttctccag gcgtcgctgc tcgaagcctc ggcccagacc tacgcccgca
     1321 ccgcgcgggc gaaaggactg cgcgaaccga tcgtctggat tcgtcacgcc ctgccgaacg
     1381 cacttctgcc gacgctggcc ttcattggtg tccagctcgg tttcctcgtc ggcggcacgg
     1441 tggtcgtcga gtccgtcttc gcctatcccg gcgtcggtcg cctcgcgctg cagtcggcat
     1501 cggaacgcga cctgccggtg gtccacgcat ttgccgtgat ggtcgccgtc gcgatcgtcc
     1561 tgatcggaac cgcgatcgac ctgatcgtcg cgatcatcga tccccggcag cgcgaacgcg
     1621 accggatgga aggagcgatc ggaattggct gatctctccg tcctcccggt tccgttggag
     1681 cgcgccagcg cgctccaacg gcgctcatcg accgatcacg agtcgcccca cgagcggcga
     1741 cgccgggcgt ggcgattgtc ctcgctctgg atgctggcgc cggttgccct cttcctgctg
     1801 ctggcgttcg gcggacccta tctggtgacc gcgagcccga ccagacagga cctgcttgcg
     1861 cgactcgcac atcccttctt cgacggtggt agctggtctc atccgttcgg gacggacagc
     1921 cttggtcgcg acatcctcgc ccggatcgcg cagggcgcgc gttattcgct gtacgtcggc
     1981 ctgatcgcgg caagcggaac cgccgtgatt ggcatcacgc tcggcatgct tgccggggcg
     2041 ggcggtcgtc ttgccgaccg cgttgtgacg ctgctctccg atgtcgtgat ggccgtgcca
     2101 ttcgtggtca tcggtatcgt gatgacggca atcctcgggc agagcctgaa gaacgtcctt
     2161 gcgatcctga tcgtttccgg ctgggtgagt tatgcccgga tcattcgact tcagacaaga
     2221 ttcctcgtgg gatctgaata tgttctcgcc tccctggcaa tgggtgcgtc gcgc
//
LOCUS       DASTMD010000893         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2852868, whole genome shotgun sequence.
ACCESSION   DASTMD010000893 DASTMD010000000
VERSION     DASTMD010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2852868"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFQ54_13225"
     CDS             <1..353
                     /locus_tag="VFQ54_13225"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0166002.1"
                     /translation="VNVSSMSAFVVNIPQTQTAYNTSKAAVTMFTKSAAIEWLPLGIR
                     VNAIAPGYFASDMTRDFVASNPEMAQEWISRVPSGRMGEPKELGPLVVYLVGDDSEYV
                     VGQSIVIDGGYTIV"
     gene            407..1171
                     /locus_tag="VFQ54_13230"
     CDS             407..1171
                     /locus_tag="VFQ54_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEU0166003.1"
                     /translation="MPSTSSLAASRRHEILLSILDTDGAIELSTAANRLDVSEMTIRR
                     DLAELETAGLVRRVRGGATRAPSGPRSFATREAQSRSAKRIIARKAASLAPEKGAVGI
                     DASTTAAVLARLLAPRKRISFVTNSWSTFSILRETPGTDASLTGGTADPTTGSLVGPL
                     SCRGAGAFSYRAFFTSASAVDPQLGSTDVSLDEAQVKQEFARSSDSVILLADSSKFHQ
                     RDLARSFSWDQIDVLVTELDPNSTRLDAYRELVAIL"
     gene            1235..>1548
                     /locus_tag="VFQ54_13235"
     CDS             1235..>1548
                     /locus_tag="VFQ54_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166004.1"
                     /translation="MTSPVAATLIARSNRLGADPRNTNYAGGNTSAKGTETDPVTGQP
                     VELLWVKGSGGDLGTLTESGLAVLRLDRMRALVDVYPGIDREDEMVAAFDYCLHGKGG
                     AAP"
BASE COUNT          294 a          502 c          453 g          299 t
ORIGIN      
        1 ttgtaaacgt gtcctcgatg tccgcattcg tcgtcaacat tccacaaacg cagacggcct
       61 ataacacctc caaagccgcc gtgacgatgt tcacgaaaag cgctgccatc gagtggctgc
      121 cgttaggcat ccgtgtcaat gcgatcgccc ccggctattt cgcgagcgat atgacgaggg
      181 atttcgtcgc gtcgaatccc gagatggcgc aggagtggat ctcccgcgtg cccagcggac
      241 gaatgggaga gccgaaagag ctcggaccgc tcgtcgttta cctcgtcggt gatgattccg
      301 aatacgtggt cggtcagtcg attgtcatcg acggcgggta cacgatcgtc tgagatctgt
      361 ctttgtcggc ttgtgaactc acgtcacatc cggaaggctt ctggtcatgc cctcgacaag
      421 ctctctcgcc gcctccagac gccacgagat actgctgtcc atcctcgaca ccgacggcgc
      481 gatcgaactc tccactgcgg cgaaccgcct ggacgtctcc gagatgacga tccgtcgcga
      541 cctcgctgaa ctcgaaacag ccggactggt tcgacgggta cgcggcggtg ccacacgagc
      601 gccttccggg ccgcgctcct tcgccacgcg tgaggcgcaa tcgcgctccg cgaagagaat
      661 cattgcgcgg aaggccgctt ccctggcccc agaaaaggga gcggtgggta tcgatgcttc
      721 cacgactgct gccgttctgg cacggctgct tgctcctcgg aagcggatct ccttcgtgac
      781 gaactcctgg tcgacctttt cgatcctgag agagacgcct ggcaccgatg cgtccctcac
      841 cggcggtacc gccgatccga caaccgggag cctggtcgga cccctcagct gtcgcggcgc
      901 aggagcgttc agctaccgag cattcttcac gagcgcgagc gccgtggatc cccagctcgg
      961 ctcgaccgac gtctctctcg acgaagcaca ggtcaagcag gagttcgccc gctcctccga
     1021 ttctgtgatc cttctcgcgg acagctcgaa gttccatcag cgcgatctcg ctcggagttt
     1081 ctcctgggat cagatcgatg tcctcgtcac agaactcgat ccaaacagca cgcgtctgga
     1141 cgcctatcgg gagctagtcg ccatcctgta atccacactt gtgtcgattg ttattttttg
     1201 taaaaataac aagatcacac acaatggagt tcatatgacc agtcctgtcg cggccaccct
     1261 catcgcacgg agcaaccgtc tcggcgcgga tccgaggaac acgaactatg cgggcgggaa
     1321 cacctccgcg aagggcaccg agaccgaccc cgtcacgggc cagcccgtcg agctgttgtg
     1381 ggtgaagggc tccggcggtg acctgggaac gctcacagaa agcggcctcg cggtgctccg
     1441 gctggaccgg atgcgcgcgc tcgtggacgt ctatcccggc atcgaccgcg aggatgagat
     1501 ggtcgcggcc ttcgactact gcctgcacgg caagggcggt gccgcgcc
//
LOCUS       DASTMD010000894         1578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_3119060, whole genome shotgun sequence.
ACCESSION   DASTMD010000894 DASTMD010000000
VERSION     DASTMD010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3119060"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFQ54_13240"
     CDS             <1..705
                     /locus_tag="VFQ54_13240"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0166005.1"
                     /translation="PAEIDRIGNLLAKHGITMSSDYGDNFMAPEKDPEEFRAFARIAR
                     QLGVHAIGLGWMPFSVNRFVDDPSFDRQMELLRTGVAPLAAIAEEEGVVLGYENHADY
                     RTSDLVEHLIEPIGSPALGIKLDTGNCPLVIEDAVEAARISAPHCFATHFKDMFISPV
                     TPDGGKIVGAPLGRGHLELDKVARILHEGVPDPENLILSIEIGWMPPNEDYFEWLAES
                     VAWCRSALAAYLSPIA"
     gene            738..1574
                     /locus_tag="VFQ54_13245"
     CDS             738..1574
                     /locus_tag="VFQ54_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0166006.1"
                     /translation="MKIGHTGITWTDLEEGYRDTAELGYMSFETFARTIVEWNGRTGG
                     YRALVDRYGIPTSAAYCYKEWIDPATAERDIDEARREADASREIGACALVLQGGSRPA
                     GGHDRDGLQRLAAALDRIGAYCREIGLVAALHPHTGTAIETRAEIDTIMEFTDPALVG
                     FAPDTGQIAKGGSDILEVLRTYRDRIEHVHLKDWIGRIERDADGKEIDLTGYAGYEAV
                     GAGALPMPEILGILETIPEHIWVNVELDGTSHSPRPPHEAAAMSRNYLGAQLGDRVAW
                     TR"
BASE COUNT          303 a          517 c          513 g          245 t
ORIGIN      
        1 cctgcggaaa tcgatcgcat cggcaacctg ctcgcgaagc acggcatcac gatgtccagc
       61 gactacggcg acaacttcat ggcgccggaa aaggatcccg aagagttccg cgccttcgca
      121 aggatcgcca ggcagctcgg cgtccatgcg atcgggctcg gctggatgcc tttctccgtc
      181 aaccgcttcg tcgacgatcc ttcgttcgac cgccagatgg agttgctccg cacgggcgtc
      241 gcgccgctgg cggcgatcgc cgaggaggaa ggcgtggtcc tcggctatga gaaccacgcc
      301 gactaccgga ccagcgacct cgtcgagcac ctgatcgagc cgatcggatc gcccgcgctg
      361 ggaatcaagc tggataccgg gaactgcccg ctcgtgatcg aggatgcggt cgaggcggct
      421 cgaatctccg cgcctcactg tttcgcgacc cacttcaagg acatgttcat cagtccggtc
      481 acgccggacg gcggcaagat cgtcggcgcg ccgctcggtc gcggtcatct cgaactcgac
      541 aaggtcgcgc ggatcctcca cgagggcgta ccggatccgg agaacctgat cctctcgatc
      601 gagatcggct ggatgccccc gaacgaggac tacttcgaat ggttggcgga atcggtcgcc
      661 tggtgtcgca gcgcacttgc cgcctacctg agcccaatcg cctgaccacg gcacgacatg
      721 aaacggggac gagcggaatg aagatcgggc acacgggaat cacctggacc gacctcgaag
      781 aggggtatcg cgatacggcg gagcttggct acatgagctt cgagaccttc gcgcgcacga
      841 tcgtcgaatg gaacgggcga accggaggat atcgggcact ggtcgatcgc tacggcattc
      901 cgacgagcgc ggcctattgc tacaaggagt ggatcgaccc ggccaccgcc gaacgcgaca
      961 tcgatgaggc gcgacgcgaa gccgatgcct cccgcgagat cggggcctgc gcgctcgtcc
     1021 ttcagggagg ctcgcgcccg gcgggtggcc acgatcgcga cgggttgcag cggctggctg
     1081 ccgccctcga tcggatcggc gcgtattgtc gcgagatcgg tctcgtcgcg gcgctgcatc
     1141 cgcataccgg cacagcgatc gagacacgcg ccgagatcga cacgatcatg gagttcacgg
     1201 atcccgcgct cgtcggcttc gcgcccgaca ccggacagat cgccaaaggt ggcagcgaca
     1261 tcctcgaagt cctcaggacc tatcgcgacc ggatcgagca cgtccatctg aaggactgga
     1321 tcggtcgaat cgagcgtgat gcggacggca aggaaatcga cctcaccgga tatgccggct
     1381 atgaggcagt cggcgcgggt gccctcccga tgccggagat cctcggaatc ctcgagacga
     1441 tcccggagca catctgggtg aacgtcgaac tcgacggcac gagccattcg ccgcgaccac
     1501 cgcacgaggc cgccgcgatg agccggaact acctcggcgc gcagctcggc gaccgggtcg
     1561 cctggacgcg atagcccg
//
LOCUS       DASTMD010000895         3833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2048561, whole genome shotgun sequence.
ACCESSION   DASTMD010000895 DASTMD010000000
VERSION     DASTMD010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048561"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFQ54_13250"
     CDS             complement(<1..456)
                     /locus_tag="VFQ54_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166007.1"
                     /translation="MAVATGVSVGSTAGVNVMLGRVAVSVGTGGSVAVGAVVSVAVGT
                     LVSVAVATGVSVGIKVSVAVGTLVSVAVGTLVSVAVAAGVSVGSTAGVNVMSTLGRVA
                     VAVGAMVSVAVGTGVLVGVSVGTGVFVGVSVGTGVFVGVSVGTGVLVGVS"
     gene            443..931
                     /locus_tag="VFQ54_13255"
     CDS             443..931
                     /locus_tag="VFQ54_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166008.1"
                     /translation="MATATETTAPTATEPSIPTDTPVATATPPVNIITPAAAPTETPG
                     ATVPAKTPDAPAPTATLQVNTVTPAPTSSACLPSGSTGATGNATPVAGTVTPGNGSGG
                     TGTSTGTGAGTGKDCGPVTALPNTGTGPTGSSPYWIPLAAAFALVAAIAGAGVRRRRS
                     AR"
     gene            1539..2534
                     /gene="accD"
                     /locus_tag="VFQ54_13260"
     CDS             1539..2534
                     /gene="accD"
                     /locus_tag="VFQ54_13260"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEU0166009.1"
                     /translation="MRNFFQRQGKFRPRDSSEDDRGDQSIPEDLWVKCPKCNELIYSR
                     ELDRNMQVCPKCDHHFRLRARQRIGYLADPGTFIEWDLNLRPEDPLHFVDGQGPYDEK
                     IVKTQQKTGEIEALITGECTVDERPLVIMVADFDFLGASMGSVWGEKMSRAVERAIEK
                     RLPVLTVNASGGARMHEGLYSLMQMAKTVSSFDQLGRASLPHFSLLVDPCYGGVTASY
                     ATVADVILAEPGAKIGFAGQRVIEQAIKQKLPKEFQTAEFMLEHGTIDFIANRQSLKQ
                     TITTLIDLYHREPAAASAPVPEPPAKVVSVNGRGSQAARAKVSAATSTKSEQSHD"
     gene            2527..3399
                     /locus_tag="VFQ54_13265"
     CDS             2527..3399
                     /locus_tag="VFQ54_13265"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEU0166010.1"
                     /translation="MTEATLPETTASGVTEIVTSTTVLTPMGRVLLARNPSRPRTLDY
                     IAQLFENFVELHGDRSFGDDRALLGGLASFRGQTVMILGHQPGGNNTQENIKRNFGMP
                     RPEGYRKAVRLMKHAEKFGFPIITFIDTPAADPGVGSEERGQGTAIAESLLAMIGTRV
                     PVICVVIGQGGSGGALAISVGDRILMLENAVYAVAPPEACAAILWKDAGKAEEAAATM
                     RITATDIAGFGIIDEVIPEPIPAHEAPRETIAAVGDRIAVHLEQLTTRNVPVETMLLE
                     RHEKFRRIGAWHEV"
BASE COUNT          718 a         1319 c         1193 g          603 t
ORIGIN      
        1 ggagacgcca accaatacgc cggtcccgac ggacacacca acgaatacgc cggtcccgac
       61 ggacacacca acgaatacgc cggtcccgac ggagacgcca accaatacgc cggtcccaac
      121 cgcaaccgag accatcgcgc cgacggctac ggccaccctg ccaagcgtcg acatcacatt
      181 tacaccggca gtggaaccga cggaaacgcc ggcggcaacc gccaccgaga cgagtgtccc
      241 gacggctacg gagacaagcg tcccaacggc caccgaaacc ttgatcccga cggagacgcc
      301 ggtggcaacc gccacggaga cgagtgttcc gaccgccacg gagaccaccg ccccaacggc
      361 gacagagccg ccggtcccga ccgaaacggc taccctgccg agcatcacgt ttacgccagc
      421 agtggaacca acggaaacgc cggtggcaac cgccactgag accaccgccc caacggcgac
      481 cgagccgtcg atcccgacgg acacgccggt ggcgaccgcg acccctccgg tcaacatcat
      541 tactccggcc gccgcgccga cggagacgcc gggagcaacc gttcccgcca agacgccgga
      601 cgctccggcc ccgaccgcga cgcttcaagt caacaccgtg acaccggcac cgacgtcgag
      661 tgcctgcctg ccatcgggtt ccactggagc aaccgggaat gccacaccag tggctggtac
      721 cgtgacgccg ggcaatggtt ccggtggaac gggtacgagc accggtaccg gcgccggtac
      781 gggtaaggat tgcggccccg tcacggcgct gccgaacacg ggtacaggac ccaccggttc
      841 ctcgccgtac tggatcccgc ttgccgcggc attcgccctg gtcgcagcaa ttgcaggagc
      901 tggtgtccgc cgcagacgca gcgcgcgcta ggaattcctg attcaacgga atgggggaag
      961 cagggattcc tgcttccccc attccgtgtc tccggacggc ccggcatcac ccccgcgttc
     1021 ctcatctcca gaccggccgc atccgggtgc ggtcgtttcc acgacggacc gacggcggcg
     1081 attccgccct cgggatcgtg atatggagac actcgatcga gagcaacctg tgcctctgcg
     1141 gcaccgaaac catcccccac gccttgatgt cgtacgtact cctaccgtat cctgttgcga
     1201 tggtccgtgt gcgtcggggg atatcctccc cggcatgctc cactccggaa tttcccgggt
     1261 ggcaacgggc gactttctgg ccacccactc gtttcgtctt caggtttcga tccaacgcgt
     1321 tcacggaacg atcatcgggg tggcacgagg ggaaccacct gttgatcctc gacgtggaat
     1381 gacagctctc gttgccacgc gtcatgtgcc cgatctttcc ccattccacg agtcaccttc
     1441 gatcgcgatc acgacgactc ccgccgctcg tcgttccagg ctgacaccct cgcggtggcc
     1501 gtgaccatca ccgatgcttg tcaggggata catcgttcat gcgtaatttc ttccagcgac
     1561 agggtaagtt tcgacctcgt gattcctccg aagacgatcg tggggatcag agtattcctg
     1621 aggatctttg ggtcaaatgc ccgaaatgta atgagctgat ctactcccgc gaactcgatc
     1681 gcaacatgca ggtctgcccg aagtgcgacc atcacttccg gctgcgcgcc cgccagcgca
     1741 tcgggtacct tgccgatccc ggcaccttca ttgaatggga cctgaatctc cggccggagg
     1801 atccgctcca tttcgtcgat ggccaggggc catacgacga gaagatcgtg aagacccagc
     1861 agaagaccgg cgagatcgag gcactgatca ccggcgaatg caccgtcgat gagcgaccgc
     1921 tcgtgatcat ggtcgccgat ttcgatttcc tcggcgccag catgggctcg gtctggggcg
     1981 agaagatgag ccgcgccgtc gagcgggcga tcgagaagcg cctgccggtc ctgaccgtga
     2041 atgcttccgg cggcgcccgc atgcatgagg ggctctattc cctgatgcag atggcgaaga
     2101 ccgtctccag tttcgatcag ctcggacggg cgtcgctccc ccatttctcc ctgctcgtcg
     2161 acccctgcta cggcggtgtc accgccagct acgcgacggt cgccgatgtg atcctcgccg
     2221 agccaggcgc caagatcggc ttcgccgggc agcgcgtgat cgagcaggcg atcaagcaga
     2281 agctgccgaa ggaattccag acggcggagt tcatgctcga gcacggaacg atcgacttca
     2341 tcgccaatcg ccagagtctc aagcagacga tcaccacgct catcgatctc taccatcgcg
     2401 agccagccgc cgcatcagcg ccggttcccg agccgccggc caaggttgtc agtgtcaacg
     2461 ggcgcggcag ccaggcggcc cgggcgaagg tcagcgcggc gacatccacc aaaagcgagc
     2521 aatcccatga ctgaggctac cctgcccgag acgaccgcaa gcggcgtcac ggagatcgtc
     2581 acctcgacca ccgtcctgac cccgatgggg agggtgctcc tggcccgcaa cccgagccgg
     2641 ccgcgcacgc tcgattacat cgcgcagctc ttcgagaact tcgtcgaact ccacggcgat
     2701 cgctcgttcg gtgatgaccg cgccttgctc ggcggtctcg cctcgtttcg cgggcagacg
     2761 gtgatgatcc tcggtcacca gcccggcggc aacaacacgc aggaaaacat caagcgcaac
     2821 ttcggcatgc cgcgcccgga gggataccgc aaggcggttc gcctgatgaa gcacgcggag
     2881 aagttcggct tcccgatcat caccttcatc gacactcccg ccgccgatcc cggggttggc
     2941 tccgaggagc gcggccaggg cacggcgatc gccgagagcc tgctcgcgat gatcgggacc
     3001 cgcgtcccgg tcatctgcgt cgtgatcggt cagggcggaa gcggtggcgc gctggcgatc
     3061 agcgtcggcg atcgcatcct gatgctggag aacgcggtct acgcggtcgc gccgccggag
     3121 gcctgcgccg cgatcctctg gaaggacgcc ggcaaggcgg aggaagccgc ggcgacgatg
     3181 cggatcaccg ccaccgacat cgcgggattc ggcatcatcg acgaggtgat tccggagccc
     3241 attcctgccc atgaggcccc gcgcgagacg atcgcggcgg tgggagaccg catcgcggtc
     3301 cacctcgagc aactcaccac gcgcaatgtc ccggtggaga cgatgctgct ggagcggcac
     3361 gagaagttcc gccgcatcgg cgcctggcac gaggtctgat cctccgatac tccatgcctg
     3421 tggcccgtcc ccacggcgct tgtcgccgtt ccggatggcg cgacggggcc ttccctgcgg
     3481 tcaggctgac gtatggcacg gcgccgcgag ggaagcacgt ggacgccgac tggtccctcg
     3541 acaagctcgg gaggacgtgt tttgggaggg cgttgtacgc acggcacgct cccgtctcca
     3601 cggcgcttgt cgccgttccg ggcagcgtcg cgcgggaagc atgtggacgc cgactggtcc
     3661 ctcgacaagc tcgggaggac gtgtctgacg ggggacaggc ccgaacgtca ggacacctcg
     3721 cgggggcgcg tggatgccaa caactcgggg ggtcgggcct gatcctgacg aggtgttgcg
     3781 atgagtgagc gctcggagcc cgatacgcca gcccggagcg gaaaccgcga ggt
//
LOCUS       DASTMD010000896         1946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_171076, whole genome shotgun sequence.
ACCESSION   DASTMD010000896 DASTMD010000000
VERSION     DASTMD010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171076"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /gene="rsmG"
                     /locus_tag="VFQ54_13270"
     CDS             <1..654
                     /gene="rsmG"
                     /locus_tag="VFQ54_13270"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU0166011.1"
                     /translation="DRNQRVNLTAVRDFPGMERRLILESLRLLPALESRIRPGNRVLD
                     IGTGGGLPGMVLAIARPDVQFTLMDATGKKVQFVLDAADALGLQNVRPLHGRAEEVAR
                     DTGHRGQYRVAVARAVSSLPTLIELGLPFVMLGGFLVLPKGIELNDELLAAEKAGKLV
                     GGNVVEAPILPDVGSSVETRLVVVEKVAPTPRTYPRRTGLPAHSPLGLADKPERPRR"
     gene            681..1289
                     /locus_tag="VFQ54_13275"
     CDS             681..1289
                     /locus_tag="VFQ54_13275"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU0166012.1"
                     /translation="MSTNAGGVERPRRPPSTRIFYRKQKLQGACWEYEIAGGWLVVPF
                     EDDVRHILHHTFTFFRPREATRRTPHGVETVLRIGAEQWFFPDRWFSLLRFTTSDDAS
                     IGYYVNFSRPLERVRENYYSDVDLELDLWLDLDGTLTELDRDEYEAEIETARLTKEWA
                     TSVDRAAEDVTAAVSRCVKDCGGDVEGTRDPVFGIPEFILRS"
     gene            1675..>1946
                     /locus_tag="VFQ54_13280"
     CDS             1675..>1946
                     /locus_tag="VFQ54_13280"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166013.1"
                     /translation="MAHLITTLGPKTDDELGMILPHEHVFVDLRTWDQPGYGEADPND
                     VIRLMAPEIERARAAGVTAIVEPGPIGVGRRADILLAVSKATNFPLV"
BASE COUNT          348 a          638 c          631 g          329 t
ORIGIN      
        1 gaccgcaacc agcgggtcaa tctgacggcg gttcgcgatt tcccggggat ggagcgtcgg
       61 ctcatcctgg agagcctgcg gctgctcccg gcgctggaat cccggattcg accgggcaat
      121 cgggtactgg acatcgggac gggcggtggg ttgccgggca tggtcctggc gatcgcgcgg
      181 ccggatgtgc agttcacatt gatggacgct acggggaaaa aggtgcagtt cgttctcgat
      241 gccgcggacg cgctgggtct gcagaacgtg cgcccactcc acgggcgggc ggaggaggtt
      301 gcccgggaca ccgggcatcg tgggcaatac cgggtcgcgg tggcacgggc tgtttcatcc
      361 ttgccgacgt tgatcgaact cgggttgccc ttcgtgatgc tgggcggatt cctggttctg
      421 ccgaagggaa tcgagctcaa tgacgagctc ctcgcggctg agaaagctgg caagctggtg
      481 ggtggcaacg tggtcgaggc accgatcttg ccggatgtcg gctcatcagt ggagacgcga
      541 ttggtggtgg tcgagaaagt cgcccccacc ccgcggacgt atccccggcg aacgggtctt
      601 cccgctcact cgccgctggg attagcagac aagccagagc gaccgagacg gtagccctgg
      661 cggctgacgg tgcatggccc atgagcacga acgccggcgg tgtggagcga ccgcgccgac
      721 caccatccac acgaatcttt tatcgaaaac agaagctgca aggtgcctgc tgggagtacg
      781 agatcgccgg tggctggctc gtcgtgcctt tcgaggatga cgtgcggcat atcctccacc
      841 acaccttcac cttctttcgc ccccgcgagg caacccgccg cacgccacac ggtgtggaaa
      901 cggtgctccg gatcggggcc gagcagtggt tcttcccgga ccgctggttc tcgctcctcc
      961 gcttcacgac atccgacgac gcgtcgatcg ggtactacgt gaatttctcc cgtcccctcg
     1021 agcgcgtacg cgagaactac tacagcgatg tcgatctcga actcgacctc tggctcgatc
     1081 tcgacggcac gctgaccgaa ctcgatcgcg acgagtacga ggcggagatc gagacggcgc
     1141 gcctgacaaa ggaatgggcg acctcggtcg accgcgcggc cgaggacgtc accgcggcgg
     1201 taagtcgctg cgtgaaggac tgcggcgggg atgtcgaagg cacgcgcgac cccgtcttcg
     1261 gcatccccga gttcatcctc cgctcctagc gagagaggcg ggacagccag ccagaggcgg
     1321 gacaggccgt ccggacctcc cgtccggcag cggcggtccc gccctacgaa cgaacggtgc
     1381 cccaaccatc cgcctccaca aattgtcatt tcgaccgaag cgaagcggag tggagaaatc
     1441 ccccggcgcg tagggcgcgg acctggtgtc cgccctccac cgcagcggac atgatcgtcc
     1501 catcgacgat gccccgccga tcctcacgat ggcgcgaaac ccgtgccggc gaccctcgtg
     1561 gtcgcctccg ggtcgcccgc aggcgactcg acctccccca ggttttacca tcgatccaaa
     1621 tgaagatgtc ctatccttgt gccagccaac acgacgcgat cgcgagggat atcgatggca
     1681 cacctgatca cgaccctggg accaaagacc gacgacgagc tggggatgat ccttccccac
     1741 gaacacgtct tcgtcgatct ccggacgtgg gatcaacccg gctacggtga agccgatccc
     1801 aacgacgtca ttcgcttgat ggcgccggag atcgagcgtg cccgcgccgc cggtgtgact
     1861 gcgatcgtcg agcccggtcc gatcggggtc ggacgccgcg ccgatatcct gctcgccgtc
     1921 tcgaaggcga ccaacttccc gctcgt
//
LOCUS       DASTMD010000897         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_352037, whole genome shotgun sequence.
ACCESSION   DASTMD010000897 DASTMD010000000
VERSION     DASTMD010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352037"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VFQ54_13285"
     CDS             <1..730
                     /locus_tag="VFQ54_13285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0166014.1"
                     /translation="YLLTIVVFVTAAALVFLTLRPDPLVLARSLRATTGAPVLKVQRS
                     IPDALRTIAAIPSAMLGFAGIVLGHSVMVAVMSMTPVHLHHGGGSLEIIGIVISLHIA
                     GMYAFSPLVGMAADRYGRKPIIALGAGILLLSFLVAGTATGHQTSQLALGLILLGLGW
                     SCTMIAGSTLLTDSIPTEQLPNVQGTSDVLMGFAGAGAGLLAGVIVGFGSYAILTIIT
                     TVMIVPLLVVALRMRIPAVRPTAA"
     gene            complement(814..1305)
                     /locus_tag="VFQ54_13290"
     CDS             complement(814..1305)
                     /locus_tag="VFQ54_13290"
                     /inference="COORDINATES: protein motif:HMM:NF020947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HEU0166015.1"
                     /translation="MQAIANNEETAGVVGFTSEEIRPDQSTRQARLKWVIVVDEALDV
                     GRMVNAAACVAAAVGFSVPGMLGEGGSDASSTFHPGLPWAGCTILKASSETIRDLQAK
                     VSGQEGFFIADMPLPAQQSRVYEEYLGELARIPAAELPLLVLSIVGPRNRVDRLVKRL
                     ELL"
     gene            1404..1877
                     /locus_tag="VFQ54_13295"
     CDS             1404..1877
                     /locus_tag="VFQ54_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU0166016.1"
                     /translation="MALDELDQAILRELQQDARKTNREVAAAVGVSATTCLDRVRSLR
                     KRGIIGGANLDVDLSRIGRPVQALISIRIRPPSRENIEAFRNWVSELPETVGVFVVSG
                     NADFLVHVAVPDNTSLYAFVIDRLTELPEVADVNTSIVYEHIRNHVIEPMQATRW"
     gene            complement(1992..>2591)
                     /locus_tag="VFQ54_13300"
     CDS             complement(1992..>2591)
                     /locus_tag="VFQ54_13300"
                     /inference="COORDINATES: protein motif:HMM:NF026049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166017.1"
                     /translation="AATVATMIHSSAFGDTNDVEDQIFVPIARAFDATRSVLDLLDAA
                     LRTASFNVDLLAVRAGEGGTTTTAIADGLVRDFGLSFRTAHHVVSRLVSEGDDITDDR
                     VLATTRDVAGQMIDVPAGWTGRMLDPWMFVDARTIHGGPAPATVEAAIANARERLAAD
                     NAWIDGAAAAIAAAAVDRAARIDAFRARERVPPMARALL"
BASE COUNT          448 a          878 c          794 g          471 t
ORIGIN      
        1 gtatctcctg acgatcgtgg tgttcgtaac cgcggcggcg ctcgtctttc tcacgttgcg
       61 ccccgacccg ctggtcctcg cgcgatccct gcgcgccacg accggcgccc cggtcttgaa
      121 ggtgcaacgt tcgattcccg atgcgttacg gacgatcgcc gcgattccat cggcaatgct
      181 tggattcgcc gggatcgtcc tcggccattc ggtgatggtc gccgtgatgt cgatgacgcc
      241 ggtccatctt caccacggcg gcggatcgct ggagatcatc ggcatcgtga tcagcctcca
      301 catcgcgggg atgtacgcgt tctctccgct cgtcggaatg gccgccgatc gctacggacg
      361 caagccgatc atcgcgcttg gcgcgggaat cctgctcctc tcgttcctcg tcgcgggaac
      421 cgcgaccgga caccagacct cgcagctcgc gttgggactg atcctgctcg gtctgggctg
      481 gtcctgcacg atgatcgccg gatccaccct gctcaccgat tcgatcccga ccgagcagct
      541 gccgaacgtg cagggaacat ccgatgtcct gatgggattc gccggtgcgg gcgcggggct
      601 gttggcgggc gtcatcgtcg gtttcggctc ctatgcgatc ctgacgatca tcaccacggt
      661 catgatcgta ccgctgttgg tcgtcgccct ccgcatgcgg attcccgccg tccgacccac
      721 cgccgcctag attccgtcct ggatcgcacg ccacgtcccg ccaagcgtcg cgacccgtcc
      781 ccgcaacagt cgcacgcccg aagagtcccc gaactacagc aattcgagcc gcttcacaag
      841 cctgtccacg cgattgcgtg gaccgacgat gctgagaacg agaagcggca actcagccgc
      901 cgggatcctg gccagttcgc caagatactc ctcatagacg cgggattgct gcgccggaag
      961 cggcatatcg gcaatgaaga acccctcctg cccggacact ttcgcctgca ggtcgcgaat
     1021 cgtctccgac gatgccttga ggatcgtgca gcccgcccag ggcagccccg gatggaaggt
     1081 cgacgacgca tcggacccgc cttcgccgag catcccgggc acgctgaatc ccaccgcggc
     1141 ggccacgcac gccgccgcat tcaccatcct tccgacatcg agcgcctcgt cgaccacgat
     1201 cacccacttc aggcgcgcct ggcgggtcga ctggtccgga cggatctcct cggacgtaaa
     1261 accgacgact ccggctgtct cttcattgtt cgcgattgct tgcatcctgg ccccctgatt
     1321 cacggaactc atgatgaaac ggtatgctga tctacggctc gtttccagaa ttccgtactc
     1381 ttcacggagg aaggtcgatt tcgatggcac tcgacgaact agatcaggcg atcctgcggg
     1441 aattgcagca ggatgcacgg aagaccaacc gcgaggtggc ggccgcggtg ggcgtctcgg
     1501 ccacgacgtg tctcgaccgg gtgcgatcgt tgcgaaagcg cggaatcatc ggcggcgcca
     1561 atctcgacgt cgatctctcg cgaatcggac gcccggttca ggcgctgatc tcgatccgga
     1621 ttcgtccgcc atcgcgcgag aatatcgagg catttcgcaa ctgggtgagt gagcttcccg
     1681 agacggtcgg cgtcttcgtc gtctccggga acgcggactt cctcgtccac gtcgcggttc
     1741 ccgacaatac gagtctctac gcgttcgtca tcgaccggct gacggagcta cccgaagtcg
     1801 cggatgtgaa cacgagcatc gtctacgagc acattcgaaa ccacgtcatc gagccgatgc
     1861 aggcgacccg ctggtagcca cgggggcagg aagggatgtc cggctgcgcg ctccacgcac
     1921 gaaagtcgtc cccgcggggc ttgtccccgc ctgccggcgc gcgaggcgtc gtgtatggat
     1981 gttgagccga gtcagagcaa ggcccgggcc atcggaggta cccgctcccg cgctcggaag
     2041 gcgtcgatcc tggcggccct gtcaacagcc gctgcggcaa tcgcggcggc ggcaccatcg
     2101 atccaggcgt tgtcagcagc aagtcgttcg cgggcattcg cgatcgccgc ttcaaccgtc
     2161 gccggcgctg gtcccccgtg gatggttcgg gcgtcgacga acatccacgg atcgagcatc
     2221 ctccctgtcc aacccgccgg gacatcgatc atctgccccg caacatcgcg ggtcgtagcg
     2281 aggacacgat cgtcggtgat gtcatcgcct tcggagacca gtcgcgatac gacgtgatgg
     2341 gcggtccgga acgagagccc gaaatcgcga acgagcccgt cggcaatcgc ggtcgtcgtg
     2401 gtgccaccct caccagcgcg taccgcgagc aggtcgacgt tgaacgatgc cgtccgcaac
     2461 gccgcatcga gaaggtccag cacgctccgc gtcgcgtcga aggcacgggc gatcggaacg
     2521 aagatctggt cctcgacatc gttggtatcg ccaaaggcgg atgagtggat catcgtcgcg
     2581 accgtcgcgg c
//
LOCUS       DASTMD010000898         4248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2495401, whole genome shotgun sequence.
ACCESSION   DASTMD010000898 DASTMD010000000
VERSION     DASTMD010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2495401"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1684)
                     /locus_tag="VFQ54_13305"
     CDS             complement(101..1684)
                     /locus_tag="VFQ54_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU0166018.1"
                     /translation="MHRSLRPIVVLMLFATLTTPLVADAQALATPASPEAQTVPPPSA
                     ITAYSIDVTLNTETNQIGGTESIDWRNTTGRAQTALYLRLYPNADYYGEASTAVERMT
                     VDGGSVTPAPGDDPTLMKIPLGKTVAADGSATISLRFTTNVPLGSNGSFGIFQLDPAN
                     GVWSLADWYPILAGWEPERKSWYTDAPTSFGDPTFSDVATYAVKLTAPASYAIASTGD
                     VHGPTGSGDAPITRTISTVPVRDFAMTLLPKTESPQGQLVQKERTVSGVDGAKPFTVR
                     VSLLKSEAIPGLEDAILAAATSALPAYAGLLSELPEDDLDITSASLAGANGVSWSGLV
                     WLDLSTIIIDRTLSDQEKIGLTFVVTHEVGHQWIGGLIGNNSNDHGFMTEGLTNALAL
                     DVLRETQGGEAAVDYLQAYVAGGYIALLRDGRDVIADAPITDDTNGVLRSLAIYGKAA
                     LGFEVIRQDLGDEAFREAMRAYASAFAYGNATPEDLLGEYTSAASPGEDVASLWNVWF
                     ERQTTKLADVDPIIAGAGD"
     gene            complement(2341..3564)
                     /locus_tag="VFQ54_13310"
     CDS             complement(2341..3564)
                     /locus_tag="VFQ54_13310"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU0166019.1"
                     /translation="MTGTIEKTNETPVPGTGYLEQTFLVGEQVYLRPIEEPDAAWTVS
                     LRDTIFPISVKYTEEWIKETVTKQEPYKEVTLAIVRKSDDRIVGSLQAHYADVAAWIS
                     ARVDPLFGDRAPVWKAEAIVLAAKWLVDEMHAATAHIELPVDEDVLVRTLTEAGFRET
                     ARWREWYFRKGNRVDRVALCYLNQGWVKTLGDPNDVELPRTGTGEARPVPVKVEIAGD
                     HPDNAMMIGKRVYLRPPTRADVEEVVRYARRETETFFDIGRHLPDVEGFAKWTEESQK
                     EKFPRWVRFAVCLRDNDEIIGEVGLFSVDLVNRTAESASFFYRPEYRGGGYGSEAKQL
                     MLEYGFNKLGLHVIQSWVYFQNTRSAAALRKQGYREAGRVNWHYMNAGTFDSFVVFDL
                     LASEWRAMPRKDWGE"
     gene            complement(3561..>4248)
                     /locus_tag="VFQ54_13315"
     CDS             complement(3561..>4248)
                     /locus_tag="VFQ54_13315"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU0166020.1"
                     /translation="DWIARLGDPREEALERTGTGVPRPVPAKVVLEGDPPKNAILIGE
                     RVYLKPIDEDDAKLMARLGRRDPETNYGHPRRLNNQGNQVAQRLPDLKERWPEHIGFS
                     VCLRENDRVIGEVAVIHIDYLNRYGESASWMYDPDYRGSGYGSEAKHLLLEYVFDTLG
                     LLAVESFVLTNNPRSAAALRKQGYRETGVINWTGTENGSFHGNIIFMLTADEWRALPR
                     VAATTGEVNS"
BASE COUNT          720 a         1376 c         1391 g          761 t
ORIGIN      
        1 atgcgcggcg tgcgaacagg ggccgagaat cggcccctgt tcgcagacaa tcgggttcag
       61 ccgggagcgt ggcgcgatcg cgtcgggccc gtccgggcga tcagtccccc gcacccgcga
      121 tgatcggatc gacatcggcg agcttcgtcg tctgccgctc gaaccagacg ttccagagcg
      181 aggccacgtc ttcccccggc gatgccgccg acgtgtactc gccgaggaga tcttccggtg
      241 tcgcgttgcc ataggcgaaa gccgaggcat aggcgcgcat cgcttcgcgg aacgcctcgt
      301 cccccaggtc ctggcgaatg acctcgaagc ccagtgcggc ttttccgtag atcgcgaggc
      361 tccgcaagac cccgttggtg tcatcggtga tcggcgcatc ggcgatgacg tcgcgcccat
      421 cacggagcag cgcgatatag ccaccggcca cgtaggcctg gagatagtcg accgccgcct
      481 cgccgccctg tgtctcccgc aacacgtcga gcgcgagggc gttggtcagc ccctccgtca
      541 tgaagccatg atcgttggag ttgttcccga tcaggccacc gatccactgg tgcccgactt
      601 cgtgcgtgac gacgaaggtg agcccgatct tctcctggtc gctcaacgtt cgatcgatga
      661 tgatcgtcga gagatcgagc cagaccagcc ccgaccagga gacgccgttt gcaccggcga
      721 gcgacgccga cgtgatatcg agatcgtcct ccggcagctc ggagagcagc ccggcgtagg
      781 caggcaacgc cgaggttgcc gcggcaagga tcgcgtcctc cagcccggga atcgcctccg
      841 acttcagcag cgacaccctg accgtgaacg gcttcgcgcc atcgacgccg ctgacggtgc
      901 gctccttctg gacgagctgg ccctgcggcg attccgtttt cgggagcagc gtcatcgcga
      961 agtcgcgaac cggtacggtc gagatcgtcc gcgtgatcgg cgcatcgccg gatccggtcg
     1021 gcccgtggac gtcgccagtg ctggcgatgg cataggaggc cggggcggtc agcttcacgg
     1081 cgtaagtcgc gacgtcactg aaggtggggt cgccgaacga tgtcggggca tcggtgtacc
     1141 agctctttcg ctccggctcc cagccggcga ggatcggata ccagtcggcg agcgaccaga
     1201 cgccgttggc aggatcgagc tggaagatgc cgaagctgcc gttggaaccg agcgggacgt
     1261 tggtcgtgaa tcgcagcgaa atcgtcgccg agccatccgc ggcaacggtc ttgccgagcg
     1321 gaatcttcat cagggtggga tcgtcaccgg gcgccggcgt gacggacccg ccatcgacgg
     1381 tcatcctctc gacggctgtc gacgcctcac cgtagtaatc ggcattcgga tagagccgca
     1441 ggtagagcgc cgtctgcgcg cgaccggtcg tgttccgcca gtcgatcgac tcggtgccac
     1501 cgatctggtt ggtctccgtg ttgagcgtca cgtcgatgga gtaggcggtg atcgcggagg
     1561 gcggcgggac ggtctgcgct tccggcgagg ccggggttgc cagtgcctgg gcatcagcga
     1621 cgagcggcgt ggtcagggtc gcgaacagca tcagcacgac gatggggcga agcgaccggt
     1681 gcatgaatcc tcttcctcgt gcggcgtcgg ttcgaccaaa gcctggccga gtcccgcgaa
     1741 ttgtagcggg ctccgtatgc cgtatgattg gtgccagttg ttggcgggag gaagagcgcg
     1801 agacagaata catcacggat tggattcgcg aggcgcggga aggtcggcat acggtcggta
     1861 cgctgccagg cggcgaggag agccagacgc gaggcgggct gaaggctccc cgcggtgctt
     1921 tccggtgcga ggcggcgcag gtcgtggaag gcgagacatt cctccgtcca gcgaccaaag
     1981 atcgtgcgga cagcgatcct gagccaggtc gctgcattcc gtagtcccgc ctccccgcgg
     2041 gcgacggatg ccttcggcac gtgaccgccg tcctccccat gcacggtgcg gacggttcat
     2101 ccaacgaccg tgacagcacg aagtacgtgg tcgaacgagc gaggacgcgc gcagccaagg
     2161 cgtgtttctg ccgaatccgc gtcgcggcgg ggtataaaac cccgccctac caaccccggc
     2221 agcatcaaac gtgtcggcgg ttccggacgt cgctatgaga ggggacggct ggcaaaggag
     2281 aagcggcggc catgatggcc gccgctcgtt cgtgctgtcg gcttcacggt gtcgccgggg
     2341 ctattcgccc cagtccttgc ggggcatcgc acgccactcc gacgcgagca gatcgaagac
     2401 gacgaagctg tcgaaggtgc cggcattcat gtagtgccag ttcacgcggc cggcctcgcg
     2461 atatccctgc ttgcgcagcg ccgccgccga gcgggtgttc tggaaataga cccagctctg
     2521 gatgacgtga agtccgagct tgttgaagcc gtactcgagc atcagctgct tggcttcgga
     2581 gccatagcca ccgccccgat attcgggacg gtagaagaag ctcgccgact ccgccgtgcg
     2641 attgacgaga tcgacactga agagaccaac ctcgccgatg atctcgtcgt tgtcccgcag
     2701 gcagaccgcg aagcgcaccc agcgcgggaa cttctccttc tggctctcct cggtccactt
     2761 cgcgaacccc tcgacgtccg gcaggtggcg cccgatgtcg aagaaggtct cggtctcgcg
     2821 acgtgcgtac ctgacgacct cctcgacatc cgcgcgtgtc ggcggacgga ggtagacgcg
     2881 cttcccgatc atcatcgcgt tgtcgggatg atcgccggcg atctccacct tgacgggtac
     2941 gggacgcgcc tcgccggttc ccgtccgcgg cagctcgacg tcgttggggt caccgagcgt
     3001 cttcacccat ccctggttga ggtagcagag ggcaacgcgg tcgacgcggt tccccttgcg
     3061 gaaataccac tcgcgccaac gtgccgtttc ccggaagcca gcctcggtca gcgtccggac
     3121 cagaacatcc tcgtcgaccg ggagctcgat atgcgccgtc gcggcgtgca tctcatcgac
     3181 cagccacttc gcggcgagaa cgatcgcttc cgccttccac acgggcgccc gatcgccgaa
     3241 aagaggatcg acgcgggcac tgatccaggc cgcgacgtcc gcgtagtgcg cctgaagcga
     3301 tcccacgatc cgatcgtcgc tcttgcggac gatcgcgagg gtgacctcct tgtatggctc
     3361 ctgcttggtg acggtctcct tgatccactc ttccgtgtat ttcacgctga tcgggaagat
     3421 cgtatcgcgg agggaaaccg tccaggcggc gtcgggctcc tcgatcggac gcaggtagac
     3481 ctgctcaccg acgaggaacg tctgctccag gtagccggtg ccggggaccg gcgtctcgtt
     3541 cgttttctcg atagtcccgg tcatgaattg acctcccctg tcgtcgcagc aacacgcggg
     3601 agcgcgcgcc actcgtcggc ggtgagcatg aagatgatgt tgccatggaa cgatccgttc
     3661 tccgtcccgg tccagttgat cacgccggtc tcgcgatagc cctgcttgcg cagtgccgcg
     3721 gccgagcgcg ggttgttggt caggacgaat gactcgacag caagaagccc gagtgtatca
     3781 aagacgtact cgaggagcag atgcttcgcc tcgctcccat agccactgcc acgatagtcc
     3841 ggatcataca tccagctcgc ggactcgcca tatcggttga gatagtcgat gtggatcacg
     3901 gcaacctcgc cgatcaccct gtcgttctcg cgaaggcaga cgctgaatcc gatgtgctcc
     3961 ggccaccgct ccttgaggtc gggcaagcgc tgcgcgacct ggttcccctg gttgttgagc
     4021 cggcgcgggt gcccgtagtt ggtctccgga tccctccgcc cgagtcgggc catcagcttc
     4081 gcgtcgtctt cgtcgattgg cttgaggtag acccgctccc cgataaggat cgcgttcttc
     4141 ggcggatcgc cttcgagcac gactttcgcg ggaaccggac gaggaacacc ggttcccgtc
     4201 cgctccagtg cctcctcgcg cgggtcgccg agccttgcga tccaatcc
//
LOCUS       DASTMD010000899         3483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2676975, whole genome shotgun sequence.
ACCESSION   DASTMD010000899 DASTMD010000000
VERSION     DASTMD010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2676975"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VFQ54_13320"
     CDS             <1..644
                     /locus_tag="VFQ54_13320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0166021.1"
                     /translation="ARTVPIGAPPAREAADDHAAEVLQIERARRDPIDFAPLYERYVD
                     AVHGYCARRVTDPELAADLTSQIFTRALTALPRYRPGITPGTFRSWLFSIAHNLVIDT
                     HRTRKETRSLDADSAPIVRDPAPTPEDHALVSDTRRALTAAMNQLTPGQRQIVELRLA
                     GLTGPEIAGVLGMQLTAVKSGQFRAYARLRELLQGHVYAGDAAGNAGEGPFDV"
     gene            637..1299
                     /locus_tag="VFQ54_13325"
     CDS             637..1299
                     /locus_tag="VFQ54_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166022.1"
                     /translation="MSEPFNAPSHGAEYDRFDRFLDSLVTGKPGGDEDEVPDLGIFAG
                     DLHQTSLPPLSTTRKRAIWNDLMSAHPSPSRTRRPGNTGPAIPPTLTIPASGPTRHRP
                     GLRPRIGDSRLGFVPPFAMRPATIVMLTIAMLLAAVAGLAGLGQSKDPGSMTPTALAS
                     EHLVVATPMPCLIASPVASPTSAGLLTSTTSTPEIASASSLTATPTGTPACAAATPPP
                     SD"
     gene            1956..2408
                     /locus_tag="VFQ54_13330"
     CDS             1956..2408
                     /locus_tag="VFQ54_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0166023.1"
                     /translation="MRDPDRAADATAAVFSRALASLPAFMPQRRADDRSSFRSWLMTI
                     ARNVAIDSIRRHPVTTTLDDPLIQSDLRDPAASPEEQAMNAFERQRIEHALATLPPIQ
                     RQIVELRVIGMSGAEIAEILQMKVGAVKTAHFRAYAKLRDLLKTGNES"
     gene            2405..>3483
                     /locus_tag="VFQ54_13335"
     CDS             2405..>3483
                     /locus_tag="VFQ54_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166024.1"
                     /translation="MNLRDILRSRKQPGADTAAIDAFFAWADEPAMPASDLLGSGLSD
                     AFSRVQSNLAMEKVVMDQSRKDALWHTLMASEATEGGERSAPASAPSGVQRRFAWGDR
                     GSDDSGSRTRRSLVIAFNALLILAVILGTTRWVQNRQSSPPELPAYIQAATTPRSGTP
                     PSDAPVYGLFPDSGCLSESNTPVPSPWVNIPDLRSALSTPISEYVGAVQRGPAPDDAS
                     LTYAPATRASDQCGQSVLNAYAVTRGSFQRSGRIAPSQSTNRYIWEHDNPYLTPAEKA
                     SLADEQNAAIAQIIPAWNPVIARTEFDFDAIWNGPYSSIKDGTVYQLADGRYASIIYK
                     RTGTTQVPGTTLVTGGNALIPYYIA"
BASE COUNT          637 a         1278 c         1005 g          563 t
ORIGIN      
        1 ccgcgcgtac cgtcccgatc ggcgcgccac cagcgcgcga ggccgctgac gaccacgccg
       61 cggaggttct ccagatcgaa cgcgcgcggc gggatccgat cgacttcgca ccgctctacg
      121 aacgctacgt cgacgctgtg cacggatact gcgcgcgtcg ggtcaccgat cccgaacttg
      181 ccgccgatct gacgagccag atcttcaccc gcgccctgac agcgcttccg cgctatcgcc
      241 ccgggattac gccggggacc tttcgcagct ggctcttttc gatcgcccac aacctggtga
      301 tcgacaccca ccggacccgg aaggagacgc gctcgcttga tgccgactcg gcgccgatcg
      361 tgcgggaccc cgccccgacg ccggaggacc atgcgctcgt cagtgacacg cgacgtgcct
      421 tgaccgccgc gatgaatcag ctcacgcctg gtcaacgtca gatcgtcgaa ctccggctgg
      481 caggtctgac cggccccgag atcgcgggag tccttggaat gcagttgacc gccgtgaaat
      541 ccggccagtt ccgggcatac gcgcgattgc gcgaactctt gcagggtcat gtctacgccg
      601 gcgatgccgc cgggaatgcc ggggagggtc cattcgatgt ctgagccatt caatgcaccc
      661 agccacggcg ccgaatacga ccgtttcgat cgcttcctcg attcactggt gaccggcaag
      721 cccggtgggg atgaggacga ggttcccgac ctcggcatct tcgccggcga tcttcaccaa
      781 acctcgcttc cgccgctctc cacgacacgg aagcgggcga tctggaacga tctgatgtcg
      841 gcccacccca gcccgagccg cacccggcgc cccggcaaca ccggcccggc catcccgccg
      901 acattgacca tccccgcgag cggaccaacc cggcatcgcc ccggactccg cccgcgaatc
      961 ggcgattccc ggctcggctt cgtgccaccg ttcgcgatgc gaccggcgac gatcgtgatg
     1021 ctgacgatcg cgatgcttct cgccgcggtc gctggcctcg ccgggctcgg tcagtcgaag
     1081 gaccccggct cgatgacacc gaccgccctc gcgagcgagc acctcgtcgt cgcgacgccg
     1141 atgccgtgcc tgatcgcttc tccggtcgcc tcgccgacat ccgccggctt gctgacgtcg
     1201 acgacctcga cgccggagat cgccagcgca agctcgctca ccgccactcc gaccggaaca
     1261 ccggcttgcg ccgccgcgac tcctcccccg tccgactgac cccctgaccg gattctccga
     1321 cgccagatgc aatgcgacgc cgagtccgac gtccacacgc tctcgaatga gcactgcggc
     1381 gatccgaaca agccgatcgg gctcaccatc ttccgccttt acgttcgcaa cgacatcctt
     1441 cccaagacga cgctatcgag acggcgcgtt cgccactccc gttcccacgg cccttgtggc
     1501 cgttctccgg gacgccgggc gggactggag tgtgtcaaga aggcgggaca gtcccgcctc
     1561 gtcctgccaa tgcccacggc cactcacaac gagacgttct gacgcaaggc tcccgttcag
     1621 ccaacgatcc gcgtcgccga agcctgtaac cttttcacgc tcccagcgtt tactcagcat
     1681 cggttcggga cgcccggagc gaggaacgat cgggagtgtc acgggatggc cgagttgtcg
     1741 ctggggaaac tcaaacgcgg tgaatccccg gattcacccg ccccgtcgcc tcgcgagacc
     1801 gcctacacgc cacatccgga ttccgcgtcc gatcttcccg tcacgctcga gaccgaactg
     1861 gccgagatcg ccgccgctca gcgcgatccc caggcgttcg ccccgatcta cgaacgctac
     1921 gtcgatctcg tctaccgcta taccccctgc gccaattgcg cgaccccgac cgggccgcgg
     1981 acgcgacagc cgccgtcttc tcccgcgccc tcgcctcatt gcccgccttc atgccacaac
     2041 gacgcgccga tgaccgcagc tcgtttcgca gctggctgat gacaatcgcg agaaacgtcg
     2101 cgatcgacag catccgccgc catccggtga cgacgacact cgacgacccg ctgatccagt
     2161 ccgatctccg cgacccggca gcttcgccgg aagaacaggc gatgaacgcc ttcgagcggc
     2221 aacgcatcga gcatgcgctc gccacgcttc ccccgatcca gcggcagatc gtcgagctcc
     2281 gcgtgatcgg gatgagcggt gccgagatcg ccgagatcct ccagatgaag gtgggcgcgg
     2341 tgaagacagc ccacttccgg gcctacgcga aactccgcga tctgctgaag accgggaacg
     2401 aatcatgaac ctgcgcgaca tcctccgatc gaggaaacaa ccgggcgcgg acaccgccgc
     2461 catcgacgcc ttcttcgcct gggcagacga gccggcaatg ccggcatccg acctcctcgg
     2521 cagcgggctt tccgatgcgt tctcgcgtgt gcagtcgaat ctcgcgatgg agaaggtcgt
     2581 catggatcag tcccggaagg acgcgctctg gcacacactc atggccagcg aagcgacgga
     2641 gggcggagaa cgatccgccc ccgcatctgc gccgagcggg gttcaacgaa gattcgcctg
     2701 gggagaccgc ggctcggatg actccggatc acgcacgcgc cgcagtctgg tgatcgcttt
     2761 caacgcactg ctgatcctgg ccgtcatcct cggtacgaca cgttgggtcc agaaccgcca
     2821 gtcctcgccg ccggaactcc cggcctacat tcaagccgcc acaacgccgc gatcgggcac
     2881 gccaccctca gacgcacccg tgtatggcct tttcccggac tcgggttgcc tatcggagtc
     2941 gaacacgcct gtccccagcc cgtgggtcaa catcccggat ctccggtccg ctctgagcac
     3001 gccaatcagc gaatacgtgg gcgcggtcca acgcggtccc gcccctgacg acgcatcgct
     3061 gacctatgca ccagcaacgc gtgcatcgga tcaatgcggc cagtcggttc tgaacgcata
     3121 tgcggtcacg cggggatcgt tccagcggag cggcaggatc gcaccgagtc agagcaccaa
     3181 ccgatacatc tgggaacacg acaaccccta tctcacaccg gcggagaaag cgtccctggc
     3241 cgacgagcag aatgccgcca ttgctcagat cattccagcg tggaatcctg tcattgcgag
     3301 gaccgagttc gacttcgacg cgatctggaa tggaccatac tcgagcatca aggacggcac
     3361 agtctatcaa ctggcagatg gtcgctatgc ctcgatcatc tacaagcgca ccggcaccac
     3421 ccaggtgccg ggaacgacac tggtgacggg cggcaacgca ttgatcccgt actatattgc
     3481 ttt
//
LOCUS       DASTMD010000900         3278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_890958, whole genome shotgun sequence.
ACCESSION   DASTMD010000900 DASTMD010000000
VERSION     DASTMD010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890958"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFQ54_13340"
     CDS             <1..216
                     /locus_tag="VFQ54_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166025.1"
                     /translation="LKAGYTALFDLEADPETLRLLPFEKAIAPTRAPKNDIRTRVRAW
                     QQRQAFEKMHHSPILRRLDDDQMACVG"
     gene            complement(465..1661)
                     /locus_tag="VFQ54_13345"
     CDS             complement(465..1661)
                     /locus_tag="VFQ54_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0166026.1"
                     /translation="MSRDLYDSLSVSQLTGVLGKKWTTFPGCIGAFIAEMDYGTAPVV
                     QDELRTAVESGFFGYVPYAKFDELAEATQSWYRDETGWEVPVSRIHAISEVLYGLELA
                     IQRFSRPGSKVIIPTPAYMPFLSLPTYLGREVVQVPLVVHDGRWEYDYDALEAAFADN
                     GGLLVLCNPSNPTGRVLERDELVRISEIVDRYDGRVFADEVHAPLIFPGQTHIPYASI
                     SAVTAGHTITSIAATKAWNIAGLKCAQLITSNDADEEIWKGIAPFALGPSTLGVVTSI
                     AAYNHARPWLQDVVDYLDGNRKVLADVVAEYLPGARYIVPEGTYLAWIDLRDLGLRGD
                     LGYFFREHAEVAVVDGRACGEVGEGFVRFNFAMPQSLVGRAIRQMGEAVARTRGTTAT
                     PAVVTT"
     regulatory      complement(1874..1981)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2164..2598
                     /locus_tag="VFQ54_13350"
     CDS             2164..2598
                     /locus_tag="VFQ54_13350"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0166027.1"
                     /translation="MAQFGIYLVFPGTTEEAFTYYKGVFGTEFAGNIARMGDMPPMEG
                     TPPLSDEEKNYIANIQLPILGGQLLLGNDMVASWGQLTVGNNFSICLMPDSREEAARL
                     FKELGEGGSVSVPLQDQFWGDYFGQVTDKFGIAWMVNYSPRG"
     gene            2887..3060
                     /locus_tag="VFQ54_13355"
     CDS             2887..3060
                     /locus_tag="VFQ54_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166028.1"
                     /translation="MMVPPDQEDQEAAQVQSPRQEPVEKELRRIAGLSAPIFWAAVII
                     LVVAVVLILFYTL"
BASE COUNT          597 a         1003 c         1097 g          581 t
ORIGIN      
        1 ctcaaggccg gctacactgc gctcttcgat ctcgaggcgg atccggagac gttgcgtctc
       61 ctgcccttcg agaaggcgat cgcgcccacg cgcgccccga agaacgacat ccggacccgg
      121 gttcgtgcct ggcagcagcg gcaggcgttc gagaagatgc atcattcgcc gatccttcgt
      181 cgtctcgacg acgaccagat ggcctgcgtg ggctgacaca ttcactgaga ccagccgatc
      241 cgtggcgatc tcggcgggac acgggaggcg ggacaggccg tccggacctg cggtccggca
      301 gcggcggtcc cgatccggag gcgggacata aaagtcccgc cctacgaacg accgctgtat
      361 ggacggcttt gtcctgaacg catcacggat cacgaccgtc cgtaatcccg taggggtcac
      421 cctcgtggtg accctgcgcg acgtccgcga aacacgtgta ttccctacgt cgtcaccacg
      481 gcgggcgtcg ccgtcgttcc cctggtcctg gcaacagcct cgcccatctg ccggatcgcg
      541 cgcccgacga gcgattgcgg catcgcgaag ttgaagcgca cgaagccctc tcccacttca
      601 ccgcaggccc ggccgtcgac gaccgcgacc tcggcgtgct cgcggaagaa gtagccgagg
      661 tcgcctcgca gtccgagatc gcggaggtcg atccaggcca ggtacgttcc ttccggcacg
      721 atgtagcgtg cgcccggcag gtattccgca acgacgtccg ccaggacctt tcgattcccg
      781 tcgagatagt cgacgacatc ctgcagccac ggacgggcat ggttgtaggc cgcgatcgag
      841 gtcacgacgc cgagggtgga ggggccgagc gcgaaggggg cgattccctt ccagatctcc
      901 tcgtcggcat cgttcgaggt gatcagctgc gcgcacttga gcccggcgat gttccatgcc
      961 ttcgtggcgg cgatcgaggt gatcgtgtgt cctgccgtga ccgcggagat cgaggcgtat
     1021 gggatgtgtg tctggccggg gaagatcagc ggggcgtgga cttcgtcggc gaagacacgt
     1081 ccatcgtagc gatcgacgat ctcgctgatg cgcaccagct cgtcccgttc cagcacgcgg
     1141 ccggtcggat tggacggatt gcagagcacg agcaggccgc cgttgtcagc gaaggcggct
     1201 tcgagggcgt cgtaatcgta ttcccagcgg ccatcgtgaa cgacgagcgg aacctggacg
     1261 acctcgcggc cgaggtaggt cgggagcgag aggaagggca tgtaggccgg ggtcgggatg
     1321 atgaccttcg agccggggcg ggagaagcgc tggattgcga gttcgagccc atagaggacc
     1381 tccgagatcg catggatccg cgagacggga acctcccagc cggtctcgtc ccggtaccag
     1441 ctctgcgtcg cttccgcgag ttcgtcgaac ttcgcgtagg ggacgtagcc gaagaagccg
     1501 gattcgacgg ccgtgcgcaa ctcgtcctgg acgacgggcg cggtgccgta gtccatctcg
     1561 gcgatgaacg ccccgataca gccggggaag gtcgtccact tcttgccgag gacgccggtg
     1621 agttgggaaa cggagaggga atcgtagaga tctctcgaca tgggatcgct catttctatc
     1681 gtgatttccg gaaggtcgct gcgcggcgtc gcgttcatgg gcgcggcggc ggttgctccc
     1741 ggaagacgga gcgatgtcgt gagatcggaa tggccgaggt cggtgccgga aggtgacggt
     1801 tgctcaaagg aaaacccctc atcttgggat gagaggcgaa aagagggcac caccgaaagg
     1861 gaggtactcg cgcccctcat cttccaggaa tgcctgccgg aattagcacc ttcccgacga
     1921 atgtcgccgg ggggttgctg gggcttcaca gggccggtcc ctctgcccct ctggatgagg
     1981 tcgaatgctc ttcagttatg ggaaggaact ctagttcctg aagcattggc cgtcaagtcc
     2041 gccgaaacca cccgggcggg ccagataccg caaggcggga gaagcgaacg ggctcgcatc
     2101 gccgatacgc tgtacgtacg gccctgcggc cgcaacgcac gacgactgaa cgaggaggat
     2161 cccatggcac agttcggaat ctatctggtt ttccccggca ccaccgaaga ggcattcacc
     2221 tactacaagg gcgtctttgg caccgagttc gccgggaaca tcgcgcggat gggcgacatg
     2281 ccgccgatgg agggcacgcc gccgctcagc gatgaggaga agaactacat cgcgaacatc
     2341 cagctcccga tcctcggcgg tcagctgctg ctcgggaacg acatggttgc ctcctggggc
     2401 cagctgacgg tcggcaacaa cttctcgatc tgcctgatgc cggattcccg cgaggaagcc
     2461 gctcgccttt tcaaggagct gggcgagggc ggctcggtga gcgtcccgct gcaggatcaa
     2521 ttctggggtg attacttcgg tcaggtgacc gacaagttcg gcatcgcctg gatggtcaac
     2581 tacagcccgc gggggtagcg cgggctcttc gttgtgagtc gacgacggag cctgccctgg
     2641 tggcagtggt gccggacttg ccgaaggggt gacccctcgc gcgcaactcg ggtgacggtc
     2701 aacgtcgacg agccatatcg caaccagaac gaggacgggg accggggcaa tcgctccggt
     2761 ccctctttgc gctgggcctg aagctgggcc gcagcgctcg aatcgggatc cacctgccgc
     2821 ggtggtctgg ttcctgcgcg gcgagcgaga aacatccatc accgaacgca tcgagaggag
     2881 atatccatga tggttccccc ggatcaggaa gaccaggaag ccgcgcaggt gcaatcgccg
     2941 cggcaggagc cggtcgagaa ggagctccgc cggatcgccg gtctctccgc gccgatcttc
     3001 tgggcggcgg tgatcatcct ggtggtcgcg gtcgtcctca tcctgttcta tacgctgtaa
     3061 cgcccacgca tacgattcgt ttgccgaaca atatgtgcgt gccgcgtgta ttgtgcgatg
     3121 attgttccat acagagaacc gatgaatacg ccgcagcacc gtgatgtgct ccggggtggc
     3181 cgtaccaccc gcttccgacc gcaattggcg gcgttcgttc ccgttccgtt tcatacggtg
     3241 cggcgccgca ggcgtacaag gagattcacc gatggttc
//
LOCUS       DASTMD010000901         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2049883, whole genome shotgun sequence.
ACCESSION   DASTMD010000901 DASTMD010000000
VERSION     DASTMD010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049883"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /gene="eboE"
                     /locus_tag="VFQ54_13360"
     CDS             <1..756
                     /gene="eboE"
                     /locus_tag="VFQ54_13360"
                     /inference="COORDINATES: protein motif:HMM:NF035939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HEU0166029.1"
                     /translation="WQFFTQRMVQMAERLVRVREADGKLIHLDLEPEPDGLLGDCAEL
                     ITFYEEWLIPLGVPLLSQRLGVSIEDAREKLLEHIRVCFDTCHVGVGFESPREVLARF
                     DELGIKVGKIQISSALKLTLDGDAGGRASQADALRPFDEPVYLHQVIQKNRDGSLTRY
                     LDLPDALPLIGDEDAVEWRIHFHVPIFIERFTAFESTQSTIIETFDVLKERPFTDHLE
                     IETYTWDVLPEGMKEDIQASIAREYEWVLNELG"
     gene            743..1642
                     /locus_tag="VFQ54_13365"
     CDS             743..1642
                     /locus_tag="VFQ54_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEU0166030.1"
                     /translation="MSSASGAGSRPATSHSPREILKAHLSLARISNTPTVVSNVLAGV
                     VMAHALALDWITIWLCIAVALFYTAGMYLNDILDIGIDRQQRPDRPIPSGAVPLPVAW
                     LTTIAMIVVGLALLLPGGGASFVAGIVLVAAIFLYDAWHKGNPVGPFIMGATRALVYV
                     TAFLAFSHDWTWEATLWSVVLLLYTAGLTSIAKTEHGPAATRYWPLVCLALPVILALA
                     RSQNAGVIILAIVFVAWAIYCCTFVYIKERRSIGGAIARLIAGISLVDALVLATHDAW
                     WGVAIAIVAFGATIFLQRWIRGT"
     gene            1646..>2698
                     /locus_tag="VFQ54_13370"
     CDS             1646..>2698
                     /locus_tag="VFQ54_13370"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HEU0166031.1"
                     /translation="MQKTVVMNVVGLVPALIGPSTPRIKAWRDKAKLSHVNPVIPAVT
                     ATVHSTYLTGKTPDQHGAVANGWYFRDMAEVKFWRQSNTLVQSEKIWDVAKKEDPTFT
                     AMNMCWWFNMNSTVDYLVTPRPMYPSDGRKIPDCYTKPIDLRDQLQAELGAFPLFTFW
                     GANANITSTKWIADASKIVDRIYNPTLSLVYLPHLDYGLQKHGPDLAAMDKDLREVDA
                     VVGDLIDFYEGKGAQVILVSEYGIMPVSRQIHLNRIFRQKGWLAYREELGRDMFDPGQ
                     SKVFAVADHQLAHIYVQDPSLLDEVKTLLESTEGVAEVLDEEGKKRYHLDHERSGELV
                     ALAEPDAWFTYYFWLDD"
BASE COUNT          482 a          831 c          837 g          548 t
ORIGIN      
        1 tggcagttct tcacgcagcg catggtccag atggcggagc gactggtccg ggtgcgcgag
       61 gcggatggca agctgatcca tctcgatctc gagccggaac cggatggcct gctcggtgat
      121 tgcgcagagt tgatcacctt ctacgaggag tggttgattc cgctcggtgt gccactgctc
      181 tcgcagcgac tcggcgtctc gatcgaggac gcgcgcgaga aattgctcga gcacattcgc
      241 gtctgcttcg atacctgcca cgttggcgtt ggcttcgaat caccgcggga ggtgctcgcg
      301 cggttcgatg agctggggat caaggtcgga aagatccaga tcagttcggc gctcaagctg
      361 accctcgacg gcgacgccgg cgggcgcgcc tcccaggccg acgcgctccg gcccttcgac
      421 gagcccgtct atctccatca ggtaatccag aagaaccgtg atggttcgct gacgcgatac
      481 ctcgatctgc cggatgcgct ccccctgatc ggtgacgagg atgcggtcga gtggaggatt
      541 cacttccacg tcccgatctt catcgagcga ttcactgcct tcgaatcgac gcaatccacg
      601 atcatcgaga ccttcgacgt gctcaaggaa cggccattta ccgatcatct tgagattgag
      661 acctacacct gggatgtcct gcccgaagga atgaaggaag acatccaggc ctcgatcgcg
      721 cgcgagtacg agtgggtgct gaatgagctc ggctagcggg gcggggagtc ggccggcgac
      781 gtcacattct ccgcgcgaga tactgaaggc gcacctgtcg ctcgcgcgga tctcgaatac
      841 gccgactgtt gtcagcaacg ttctcgccgg tgtggtgatg gcgcacgcgt tggcgctcga
      901 ctggatcacg atctggctct gcatcgccgt cgcgctgttc tacacggccg ggatgtacct
      961 taacgatatc ctcgacatcg gcatcgatcg ccagcagcgg ccggatcgcc cgattccctc
     1021 cggcgccgtg ccgcttcccg tcgcgtggct gaccacgatc gcgatgattg tcgtcgggct
     1081 ggcactcctt ctgcccggag gcggggcgtc cttcgtcgcc ggcatcgtgc tcgtggcggc
     1141 gatctttctc tatgacgcgt ggcacaaggg aaatccggtc ggccctttca tcatgggcgc
     1201 caccagggcc ctcgtctacg tcacggcgtt tctcgccttc agtcatgact ggacgtggga
     1261 ggcgaccctc tggagtgtcg tgctcctgct ctatacagcc ggcctgacat cgatcgcgaa
     1321 gacggaacac ggtccggcgg cgactcgtta ttggccgctt gtctgcctgg cgctgccggt
     1381 gatcctcgcc ctcgcaagat cgcagaacgc gggcgtcatc attctcgcca tcgtctttgt
     1441 tgcatgggcg atctactgct gcacgtttgt ctatatcaag gaacgccgga gtatcggcgg
     1501 ggcgattgcc cgcctgatcg ccggcatttc gcttgtcgac gcgctcgtgc ttgccacgca
     1561 tgacgcgtgg tggggtgtcg cgatcgcgat cgttgccttc ggcgccacga tctttctcca
     1621 gcgctggatt cgaggaacat aagccatgca gaagactgtt gtgatgaatg tggtcgggct
     1681 cgttcccgcg ctgatcggcc cgtcgacgcc gcgaatcaag gcgtggcggg acaaggcgaa
     1741 gctttcgcac gtcaatcccg tgatccccgc ggtcacggcg acggtgcatt caacctatct
     1801 caccggcaag acgccggacc agcatggcgc ggtggcgaac ggctggtact tccgcgacat
     1861 ggccgaggtc aagttctggc ggcaatcgaa caccctggtg cagtcggaaa agatctggga
     1921 tgtcgccaag aaagaggatc cgaccttcac cgcgatgaac atgtgctggt ggttcaacat
     1981 gaactccacc gtggactatc ttgtgacgcc gcggccgatg tatccatcgg acgggcgcaa
     2041 gatccccgat tgctacacga agccgatcga ccttcgtgac cagctgcagg cggagctggg
     2101 ggccttcccg ctcttcacgt tctggggcgc gaacgccaac atcacgtcga ccaagtggat
     2161 cgcggatgct tcgaagatcg tcgaccggat ctacaatccg acgctctcgc tggtctatct
     2221 gccgcacctc gactacggcc ttcagaagca tgggcccgat ctggcggcga tggacaagga
     2281 tctccgtgag gtcgacgcgg tggtcggcga cctcatcgac ttctatgagg gcaagggcgc
     2341 gcaggtcatt ctcgtctccg agtacgggat catgccggtc agccgccaga ttcacctgaa
     2401 tcgaatcttc cgccagaagg ggtggctcgc ctaccgcgag gaactcggtc gcgacatgtt
     2461 cgaccccggt cagagcaagg tcttcgcggt cgccgaccat cagctggcgc acatctacgt
     2521 gcaggatccg tcccttctcg atgaggtaaa gacgctcctc gagtcgacgg agggcgtcgc
     2581 cgaggttctc gatgaagagg gcaagaagcg atatcacctc gatcacgagc gatcgggcga
     2641 actggtcgcc ctggccgaac ccgatgcctg gttcacatac tacttctggc tcgatgac
//
LOCUS       DASTMD010000902         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2763763, whole genome shotgun sequence.
ACCESSION   DASTMD010000902 DASTMD010000000
VERSION     DASTMD010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2763763"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFQ54_13375"
     CDS             complement(<1..352)
                     /locus_tag="VFQ54_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166032.1"
                     /translation="MVAVIVLVGLGTSVAEGAQSTTRTSPTAYGKRAEVGDYEVRLKS
                     VNFNAARWLQEQDSHVDSPAAGNVYVVADVELTYKGATSGTPAFDLEFKAVGDKNRGY
                     DQSNSDCGSIPNELY"
     gene            complement(698..1750)
                     /locus_tag="VFQ54_13380"
     CDS             complement(698..1750)
                     /locus_tag="VFQ54_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0166033.1"
                     /translation="MTKHGSFKKVVRRHAEATGQRYTEALTDLEGLDDRMIHEPSAEH
                     LLAHIRDRYRIDATAATKISLHKTYLFRIDRTDGDPWIARAFPPARPRSGVEGDAAIL
                     RFLERQDFPAERLAVDDAVSDFAGSTVLVTRFVENKPFPKVSEKLGMMGDLLGRLHAL
                     PLDESVSRPGGASGEDPAHEGSPRQDLMAALAFLDAVDTKVAASERERFEYLRELVLS
                     ADDGDGLPEALIHGNMLHNPDHALLTDHGPVGINWTSAGRGPRLADFAWLMWGTWRHP
                     DAIDAAVSGYRRHIELTDEELDRFEAVMYLRPLFLAGFIIRRNILEGLTEDALAFVDP
                     EYLRATAEATRNAFRR"
     gene            complement(2070..>2305)
                     /locus_tag="VFQ54_13385"
     CDS             complement(2070..>2305)
                     /locus_tag="VFQ54_13385"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0166034.1"
                     /translation="GVTGALRIAHLAQAYHMRAEVHGGGAPNLHIACAIPNTTYYESL
                     VRTNPIKVEAGIGPDGCISPPEVPGIGWDAMPE"
BASE COUNT          415 a          741 c          713 g          436 t
ORIGIN      
        1 tatatagttc attcgggatc gagccacaat cgctgttgct ttgatcgtac ccccgattct
       61 tatccccgac cgctttgaac tcgagatcga atgccggtgt gccggaagtc gcgcccttgt
      121 atgtcagttc gacatcggca acgacgtaga cgttcccggc tgcaggagaa tcgacatgac
      181 tatcctgctc ctgcagccaa cgtgcggcat tgaagttgac cgatttcagg cgaacttcgt
      241 aatcgccgac ctccgcacgc ttgccatatg cggtcggcga tgtacgcgtg gtcgactgcg
      301 caccctccgc gaccgacgtg ccgagcccaa ccaacacgat gacggcgacc atgaatgtgc
      361 gcacgcaacg ccagatcatg tctacccctc cccatgaaaa ggactgactt ccggctgaaa
      421 tcagccggga atgtacggcg tatgtaccga cggcatttct acgctagaac ctggtgacca
      481 gagtctcatc atagcgctgt aaacaagcaa ttcatatcgc tcggacaagg caagagcgga
      541 cccggagttc gtcgcctccc ggtccgctgc aggttgtcgc tcgtcgatcg tcctagctca
      601 tcgcgtccca accgatgccc ggcacctccg acggggagat gcagcctccc ggtgaatccg
      661 gcatcagtcc agatggtgtt cgggatcgcc atggccgcta gcggcgaaac gcgttcctcg
      721 tcgcctctgc ggttgcacgg aggtattcgg gatcgacaaa ggccaacgcg tcttcggtga
      781 gtccctccag gatattgcgc cggatgatga agcccgccag gaacagtggc cgcaggtaca
      841 tgaccgcctc gaaccggtcg agttcctcat ctgtcagctc gatgtgtcga cggtacccgc
      901 tcacggccgc gtcgatggcg tccgggtgac gccaggtgcc ccacatcagc caggcgaagt
      961 cggcgagccg tgggccgcgt ccggcggacg tccagttgat ccctaccgga ccgtggtcgg
     1021 tcagaagcgc atgatcggga ttgtggagca tgttgccatg gatcaacgcc tccggcagtc
     1081 cgtctccgtc gtccgcagag agaaccagct cgcgcaggta ctcgaatcgc tcgcgttcgg
     1141 acgctgcgac tttcgtatcg accgcgtcga gaaacgcgag ggccgccatc aggtcctgac
     1201 gtggacttcc ttcatgggcg ggatcctcgc cgctggcccc accgggacgg ctcacggatt
     1261 cgtccaacgg cagcgcatgg agccgcccga gcaggtcccc catcatgccg agcttttcgc
     1321 tgaccttcgg gaacggtttg ttctcgacaa accgggtgac gagcaccgtg ctgccggcga
     1381 agtccgacac cgcgtcgtcg acggcaaggc gttccgcggg aaagtcctgt cgttccagga
     1441 aacggaggat ggcggcgtcg ccctcgacgc cagatctggg gcgagcgggg gggaacgcgc
     1501 gtgcgatcca cggatcgcca tcggtccggt cgatacggaa gaggtaggtt ttatgcagac
     1561 tgatcttggt tgccgcggtg gcgtcgatgc ggtagcgatc ccgaatatgc gcgagcaggt
     1621 gctcggcgga gggctcgtgg atcatccgat cgtcgagccc ctcgagatcg gtgagtgcct
     1681 cggtgtagcg ttgcccggtt gcctcggcgt gacgccggac aaccttcttg aaactgccgt
     1741 gcttggtcat gaacaagcct cctcgataca ccctcgtccc ccagcgacgc ggtgtttgga
     1801 agatggcctg tgatacgcat gaccgggagg gcagattccc cttcgccatt ggaaccccag
     1861 ctggggcgga tgctccacgg ctcggcgact ggtcggcgcc gtcgcgccca tcctagggtg
     1921 atcgtggcct caagtcaacc gctaccactc cacagcgttg accgtctccg gcgagctgcc
     1981 ccgaccggga accacgaaca aggcggggtg agccaggatt gctcccatcc accccgttcg
     2041 ccatgaacaa cgtgtttctg gataactgcc tactccggca tcgcgtccca gccgatgccc
     2101 ggcacctccg gtggcgagat gcagccgtcc ggaccgatgc cggcctcgac cttgatcggg
     2161 ttcgtccgca ccagcgactc gtagtaggtc gtgttcggga tcgcgcaggc gatgtgcagg
     2221 ttcggcgcgc cgccaccgtg gacctccgcc cgcatgtgat aggcctgcgc gagatgcgcg
     2281 attcgtagcg cgccggtgac gccac
//
LOCUS       DASTMD010000903         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2141857, whole genome shotgun sequence.
ACCESSION   DASTMD010000903 DASTMD010000000
VERSION     DASTMD010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141857"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..2083
                     /locus_tag="VFQ54_13390"
     CDS             254..2083
                     /locus_tag="VFQ54_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0166035.1"
                     /translation="MSDESRDQKAGVAKRRTGINRRSFIAAGAGASAAAIGTALQAGA
                     QEATPAASPVPPLTTPATWDYEADVVVVGGGAAGHAAVVTALNDGSEVILVEKNGVTG
                     GTSVLAYSYWIPNNPYMQAAGLTDPKADAIKYMARLAYPTLYNPAQDFLGLTAHEYSL
                     LEAFYDNGYEAIELFDKLGALKSVIAPSYGFSTMPKLADPDYHADLPEDKAPFGRSIT
                     GAGSDPTTSHYMAQLKAYVASKKVTQLVSHAATGLFRNSAGAVVGVQADNQGKTVTIR
                     ARKGVIFASGGFTQNPEKALNYLRGPIFGGCGIPTNQGDFIDIGIEAGAQLGNMNNAF
                     WVQNVVEATMQNPSTTSDVWLPYGDSMIIVNKYGHRVTNEKMVYNERTQTHFYWDAPR
                     REYSNLVLFMIWDDAVAKNTVNWPYRFPVPLPTDPTPDYILKGETWEDLAKAIDDRLA
                     KISGKRTISAQIAPSVKLADNFTSELAKTVATFNGYAEAGEDPEFLRGTTPIQIAWQG
                     PPREGNTKNPTMYPIAGKGPYYAVILGGGTLDTKGGPVVNASAQVLALDGAPIVGLYG
                     AGNCIASPAGQGYFSAGGTVGPGMTYGYIAANHASGQPENPVS"
     gene            complement(2183..>2848)
                     /locus_tag="VFQ54_13395"
     CDS             complement(2183..>2848)
                     /locus_tag="VFQ54_13395"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEU0166036.1"
                     /translation="GGYKSSSNNVAAFDEDGNQVGIILETATPFDTPRLMAELVAWYR
                     DSSLDVTLHPLLAVGIFVVAFLEIHPFQDGNGRLSRILTTLLLLQTRYAYVPYSSLEH
                     VIEQSKDRYYLALRRTQGTIRSASPDWHPWLIFFVRALHQQMRRLADRIERERLILSP
                     LPPIASRIVEHAREHGRVTIGEMAVLTGVSRNTLKVHLRNLVHDGHIAQHGAGRGTWY
                     SLP"
BASE COUNT          575 a          853 c          915 g          505 t
ORIGIN      
        1 ctgctatcag atttctatgg cggccgacgg tctccgcttc tttgctcaag gcggcgaggc
       61 gatgcgcgac gcgtacaact ggctgctccc gagggtccag agactgctct cccgccaata
      121 gatacgacaa ctgctgcata agtactcgtt ccgttacttg cgatgacgaa ggtagcaggc
      181 gaagatgacg ccgcatcata catatgcaac gggtccgtga ataactgtcg aaatgtcgaa
      241 ggagacgttc ggaatgagtg acgaatcacg ggatcaaaaa gccggcgtgg cgaagcgccg
      301 gaccggtatc aatcgtcgta gtttcatcgc ggccggcgcc ggagccagcg ccgcggcgat
      361 cggaaccgca cttcaggccg gcgcgcagga agcgactccg gctgcttccc cggtgccacc
      421 attgacgaca ccggcgacgt gggattacga agcggatgtc gtcgtcgtcg gtggtggggc
      481 ggccggtcac gctgccgtcg tcaccgcgct gaacgacggg tccgaggtca tcctcgtcga
      541 gaagaacggg gtcaccggcg gcacgtcggt gctcgcctac tcctactgga tcccgaacaa
      601 tccgtacatg caggcggccg gcctgaccga tccgaaggca gacgcgatca agtacatggc
      661 gcgtctcgcc tacccgacgc tctacaaccc ggcccaggat ttccttggcc tcaccgcgca
      721 tgagtacagc ttgctcgagg ccttctacga caatggctac gaggcgatcg aactcttcga
      781 caagctggga gccctgaagt ccgtgatcgc gccatcgtat ggcttcagca cgatgccgaa
      841 gctggccgat cccgactatc acgccgatct cccggaggac aaggcgccgt tcggacgcag
      901 catcaccggc gccggttccg atcccacgac gagccactac atggcgcagc tgaaggcgta
      961 cgttgcctcg aagaaggtca cccagctcgt cagccatgcc gcgaccgggc tcttcaggaa
     1021 tagtgccggc gccgtcgttg gcgtccaggc ggacaatcag ggcaagacgg tcacgatccg
     1081 cgcacgcaag ggcgtgatct tcgcctccgg tggtttcacc cagaatccgg agaaggcgct
     1141 caattacctt cgcggtccga tcttcggtgg ctgcggcatc ccgaccaacc agggtgattt
     1201 catcgacatc gggatcgagg cgggcgccca gctcggcaac atgaacaacg ccttctgggt
     1261 gcagaatgtc gtcgaggcga cgatgcagaa cccgagcacg acgagcgatg tctggctgcc
     1321 gtacggcgac agcatgatca tcgtcaacaa gtacggccat cgcgtgacga acgagaagat
     1381 ggtctacaac gagcggacgc agacccactt ctactgggat gcgccgcgtc gtgagtattc
     1441 caatctcgtg ctcttcatga tctgggatga cgccgtcgcg aagaacacgg tcaactggcc
     1501 gtaccgcttc ccggtgccgc tgccgaccga tccgacgccg gactacatcc tcaagggcga
     1561 gacctgggag gacctggcga aggcgatcga cgatcgtctc gcgaagatca gtggcaagcg
     1621 cacgatctcg gcccagatcg cgccgagcgt gaagctcgcc gacaacttca cgagcgagct
     1681 cgcgaagacg gtcgccacct tcaacggcta tgcggaagcg ggcgaggatc cggaattcct
     1741 ccgcgggacg accccgatcc agatcgcatg gcaggggccg ccgcgtgaag gcaacaccaa
     1801 gaaccccacg atgtacccga tcgccgggaa gggcccgtac tacgcggtga tcctcggtgg
     1861 aggcacgctc gatacgaagg gcgggccggt cgtcaacgca tccgcgcagg tgctggcgct
     1921 ggacggcgcg ccgatcgtcg gtctctacgg cgcggggaac tgcatcgcct caccggctgg
     1981 ccagggatac ttctcggccg gtggcacggt cgggccgggc atgacctacg gctacatcgc
     2041 ggcgaatcat gcgtctgggc agccggagaa cccggtcagc taggccatct cgacaatttg
     2101 tcggcaagag gaagactccg gatctcgcat agagatcagg ggttatccgc gtgtgacgat
     2161 ggggcttttg taccgaagcc gattacggga ggctgtacca cgttcctcga cccgcgccat
     2221 gttgtgcgat atggccgtcg tgcacgagat tgcgaagatg aaccttgagg gtgttgcggc
     2281 ttacgccggt gaggacggcc atctccccga tcgtgacacg accgtgctcg cgggcgtgtt
     2341 cgacaatcct gctggcgatc ggtggaagtg gcgagagtat cagtcgctcg cgttcgatgc
     2401 ggtcggcgag gcgccgcatc tgttgatgga gagcgcgcac gaagaagatc agccaggggt
     2461 gccagtctgg cgacgctgaa cggatcgtcc cctgggttcg acgaagcgcg aggtagtagc
     2521 ggtccttgct ttgctcgatg acgtgttcga gtgaactata tggcacgtag gcgtatctag
     2581 tctggaggag tagaagtgtc gtcagaatgc ggcttaggcg tccattgcca tcctgaaaag
     2641 gatgtatctc aagaaatgcg acgacgaaga tgccgacggc caggagtgga tgcaatgtca
     2701 cgtccagcga ggagtcgcga taccaggcga cgagctctgc catcaagcgg ggtgtatcga
     2761 agggtgttgc cgtctcgagg atgatcccga cctgattgcc gtcttcgtcg aaggccgcga
     2821 cgttgttcga tgaggacttg tacccccc
//
LOCUS       DASTMD010000904         1968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2050063, whole genome shotgun sequence.
ACCESSION   DASTMD010000904 DASTMD010000000
VERSION     DASTMD010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050063"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="VFQ54_13400"
     CDS             <1..1575
                     /locus_tag="VFQ54_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0166037.1"
                     /translation="VRLLNGPAMAQEVQVAATPVALGPKVPPELTQKGAWPVEGKDLA
                     MTRYVDDSNISAATVDTLGVAWSFPIDVTSGSGAITSNPIVLGNTVYIEDMLSNIVAI
                     DIDSGKQIWRTEFNTTSEGPNGIALGYGLAVSVLGDTSEVVALDAATGKLKWRTDLSS
                     NIGEGIDMAPIVYDNTVYVSTVPGNSNAFYRGGQKGIFYALDISSGDVIWQWDTTVDN
                     LWGNARVNSGGGLWHPPSIDDDGMLYLGVANPGPWPGNSEFPNGSSRPGDNLYTNSVV
                     KLDPSTGSVVWYVQIVPHDLFDLDNQLSPVLTTVKIGGVDTGIVVTSGKHGQVVAVSR
                     DGQELWRTAIGKHENDNLKAIPDGKTIEEYPSSLGGVETPFSIANGVGFFATMNLARY
                     YTSTGKSTTVTPVAIGDATGEVVAIELTTGKILWDVVVPTAPFAGTTVVGDLVFAGGL
                     DGVLRAFNVKDGSEVWKWQAPAGINATLAVVGDYLFLGAGGALLASKDTASPTPTPAS
                     ALVALKLGASKATPAS"
     gene            complement(1733..>1968)
                     /locus_tag="VFQ54_13405"
     CDS             complement(1733..>1968)
                     /locus_tag="VFQ54_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166038.1"
                     /translation="EFPIVVVSLRQDRTQSRVEGDERQESNLLARRVNHMAMTRAMRS
                     LLVTLPLSDNEHTSGITSPLWTIIDTRRDGGAE"
BASE COUNT          364 a          632 c          639 g          333 t
ORIGIN      
        1 gtccgccttc tcaacggacc ggcaatggcg caggaagtcc aggtcgcggc gaccccggtc
       61 gcgttggggc cgaaggtccc gcctgaactc acgcagaagg gcgcctggcc ggtcgagggg
      121 aaggacctcg ccatgacgcg ctacgtcgac gacagcaata tcagtgctgc cacggtcgac
      181 acgctcggtg tcgcctggag ctttccgatc gacgtgacgt cgggctcggg tgcgatcacc
      241 tcgaatccga tcgtgcttgg caacaccgtc tacatcgagg acatgctgag caatatcgtg
      301 gcgatcgaca tcgactcggg caagcagatc tggcggaccg agttcaacac gacgtccgag
      361 gggccgaacg gtatcgccct tggatacgga ctcgcggtca gcgtgctcgg cgatacctcc
      421 gaggtcgtcg ccctcgacgc ggccaccggc aagctcaagt ggcgcaccga tctttccagc
      481 aacatcggcg aaggcatcga catggcgccg atcgtctatg acaacacggt ctacgtcagc
      541 acggtgccgg gcaactcgaa cgccttctat cgcggcggcc agaagggcat cttctacgcg
      601 ctcgacatca gctccggcga cgtgatctgg cagtgggaca ccaccgtcga caacctctgg
      661 ggaaacgcgc gcgtcaattc cggtggcggc ctctggcacc caccctccat cgatgacgac
      721 ggaatgctct atctcggcgt cgcgaacccg ggtccgtggc cgggaaactc cgaattcccc
      781 aatggctcca gccggccggg tgacaatctg tacaccaatt ccgtggtgaa gctggatccc
      841 tctaccggaa gtgtcgtctg gtacgtgcag atcgttccgc acgatctctt cgatctcgat
      901 aaccagctct cgcctgtcct gaccacggtc aagatcggtg gcgtggacac gggcatcgtc
      961 gtgacgtccg gcaagcacgg ccaggtcgtc gctgtcagtc gcgacggcca ggaactctgg
     1021 cggaccgcga tcgggaagca cgagaacgac aatctcaagg cgattccgga tgggaagacg
     1081 atcgaggaat atccaagctc gctcggtggc gtcgagacgc cgttctccat cgcgaacggg
     1141 gtcgggttct tcgcgacgat gaacctcgcg cgctactaca cgtcgaccgg aaagtcgacg
     1201 acggtgacac ccgtcgcgat cggggatgct accggcgaag tcgtcgccat cgagctcacg
     1261 acggggaaga ttctctggga tgtcgtggtt ccgacggcgc catttgccgg gaccaccgtt
     1321 gtcggcgatc tcgtgttcgc cggcggtctc gatggcgtcc tgcgggcgtt caacgtgaag
     1381 gacggcagcg aggtctggaa gtggcaggcg ccggccggaa tcaacgcgac gctggccgtc
     1441 gtcggtgact acctcttcct tggcgcgggt ggggcgctgc tcgcatcgaa ggacacggca
     1501 tccccgacgc cgacgccagc ctcggcgctg gtggcgctca agcttggcgc cagcaaggcg
     1561 acaccggcgt cgtagcgttt gccggcggct cgatatcgaa caggggccgg cgagaggcgg
     1621 gacaggccgt ccggactgcg gtccggcagc ggcggtcccg ccttacgaac gaaccccgtc
     1681 atggccgtcc gccagccgta tgacggagaa catggcaacg tcgccgcggg cgtcattcag
     1741 caccgccgtc gcgccgggta tcgatgatcg tccagagggg agacgtgatc ccggacgtgt
     1801 gctcgttgtc cgaaagcgga agggtcacca ggagcgaacg catcgcacgc gtcatcgcca
     1861 tgtgattgac cctccgtgcg agcagattcg attcctggcg ctcgtcgccc tcgacacgcg
     1921 actgcgtccg atcctgtcgc aacgagacca cgacgatcgg gaattcca
//
LOCUS       DASTMD010000905         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1335416, whole genome shotgun sequence.
ACCESSION   DASTMD010000905 DASTMD010000000
VERSION     DASTMD010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335416"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="VFQ54_13410"
     CDS             complement(<1..1559)
                     /locus_tag="VFQ54_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU0166039.1"
                     /translation="MSQNMIYAIEMSQLFADQFRILPRDQAALVQSKIALLQDDPRPV
                     GKNKTRLAGMNVPPRFRIRAGNYRVIYSFTPDTIWLWNVDKRSDVYVRHIAARYVPVA
                     ESTIADNHFVELRDDTSATGASWAHYQQGLREWGAGEHPQRRNHKPADHRSDLHPATR
                     LPSRITTEMLHRIAIPERYHRALISCETDDDLVRAVTEWGIPAEAADRVFSLVVDPDF
                     EYIRHQPRKQLKRVEDLTRFHSGDLLDFMVALDAEQRQVAKSVAENRGPALISGFAGT
                     GKSAVLLEIAIALASADPDRRILFTTFTAGLLANTEELLRRVAPHLLPQFAFRTTSSV
                     AGKLLGPATESGDRELLEEASQQRILREIVRDLVLNWTPARQLANFVPRRISLSGSLI
                     EAGPLAGMSWNYLLQEINQVIIGRGIDQELDYLTEPRDGRRVGLNEGQRSILWEISHR
                     FVRAIREQHATTYPLLVLDASRAVAQSRLKSEYDAVFVDEAQDLSPAAIRMLVELARG
                     GDGLVRDLYLSA"
     gene            complement(1938..>3142)
                     /locus_tag="VFQ54_13415"
     CDS             complement(1938..>3142)
                     /locus_tag="VFQ54_13415"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU0166040.1"
                     /translation="EVFNQTEPDYILEVCKRMTDLWDAAPERPVILNLPATVEIATPN
                     VYADQIEYMHRNLDRRESVILSIHPHNDRGTGVACAELAMMAGAERIEGCLFGNGERT
                     GNVDLVTLALNLYAEGIDPQLDFSDIDAIQRTVEACNRIGIHERHPYAGELVYTAFSG
                     THQDAIRKGFAHHETRAAELGVASSAAPWDIPYLPIDPADVGRDYEAVIRVNSQSGKG
                     GIAWVVEQELGLEMPRALQVNFSRVVQAETDTTGEEIAASRLHDLFLETYVNLEGPVA
                     LEEWSLQAASGEDHLVATLRIGGERITATGVGNGPVSALTGILRARGIALDIEQLTQQ
                     SLTHGRDSKAATFIEARIGDRIGWGVGIDSSVLTASLQAVIAAANRIGIIDATRNRER
                     EAGHYSDQ"
BASE COUNT          552 a          935 c         1048 g          607 t
ORIGIN      
        1 gcggagaggt agagatcgcg aacgagtccg tcgccgccgc gcgccagttc gacgagcatc
       61 cggatcgcgg cgggactcag gtcctgcgcc tcatcgacga agacggcgtc gtattccgat
      121 ttcaatctcg attgggcaac cgcccgtgat gcgtccagta ccaggagcgg ataggttgtc
      181 gcgtgttgtt cgcggatggc gcggacaaag cggtggctga tctcccaaag aatggaacgc
      241 tggccctcgt tcaggccaac acgccggcca tcgcgtggct cggtcaggta gtcgagctcc
      301 tggtcgatgc cgcgtccgat gatgacctgg ttgatctcct gcagcaggta gttccagctc
      361 atcccggcga gcgggcccgc ttcgatcagc gacccactga gcgagatgcg ccggggcacg
      421 aagttggcga gttgacgtgc tggagtccag ttcaggacga ggtctcgcac gatctcgcgg
      481 aggatgcgct gctggctggc ctcctccaga agttcgcgat ccccggattc ggtggcggga
      541 ccgaggagtt ttcccgcgac actcgaggtc gtgcgaaacg cgaactgcgg gagcaggtgc
      601 ggggcgactc gtcgcagcaa ctcctcggtg ttcgcgagga gtccggccgt aaaggtcgtg
      661 aagaggatcc gccgatcggg atcggcgctc gccaacgcga tcgcgatctc gagcagcact
      721 gccgactttc cggttcccgc gaagccggag atcagcgcag gtccgcgatt ttccgctact
      781 gacttcgcga cctggcgctg ttccgcgtcg agcgcgacca tgaaatcgag cagatccccg
      841 ctgtgaaagc gtgtgagatc ctcgacccgc ttgagttgct ttcgtggctg gtggcgaatg
      901 tactcgaaat ccggatcgac aacgagactg aagacgcgat cggccgcttc ggccggaatc
      961 ccccattccg tcaccgcgcg cacgagatcg tcgtcggttt cgcaggagat cagggcacga
     1021 tgatagcgtt ccggaatcgc aatgcgatgg agcatctctg tggtgatccg gctcggaagg
     1081 cgggtggcgg ggtggagatc agatctgtga tccgccggct tgtggttccg gcgttgcgga
     1141 tgctcgcccg caccccactc gcggagaccc tgctgatagt gtgcccagga tgcgccggtc
     1201 gcggacgtgt catcccgaag ttccacgaag tggttgtccg cgatggtcga ttcggcgacg
     1261 ggaacgtagc gagcggcgat atggcggacg tagacgtcgg agcgcttgtc gacgttccag
     1321 agccagatcg tatccggtgt gaaggaatag atgacgcgat agtttccggc gcggatgcgg
     1381 aagcggggcg gcacgttcat gccggcgagg cgagtcttgt tcttgccgac ggggcgagga
     1441 tcgtcctgca acagggcgat cttgctctgg accagcgctg cctgatcgcg cgggagaatc
     1501 cggaattgat cggcgaagag ttgcgacatc tcgatggcgt agatcatgtt ctgcgacatt
     1561 ggattccgcg atcattccgg gggtgagctt ctacagggtt gatgatccga ttccgatgac
     1621 cggccggcca acccgcgtcg tgccaaccga tcgatcacga tttgcataca ttgtcatgca
     1681 atccgcatcc cgggtcgatg tcatcaacga gatatgtatc ttcccgtctc aaaggcggga
     1741 catggaagtc ccgccctacg gacagccgcc gtattacaac gcccatgcgt agggcgggta
     1801 ggatagcgct gcctcagagg cgggacatgg aagtcccgcc atactcacag acgcctcgca
     1861 tacagcgttc gttcgcaggg cgggactgcg gagaccaatg accggcgacc gggatgcctt
     1921 gcgggtccca atcacggtca ttggtcggag taatgtcccg cctctctctc ccggttccgc
     1981 gttgcatcga tgataccgat ccggttcgcg gcggcgatca ccgcttgcag cgaggccgtc
     2041 aggacggacg aatcgatgcc aactccccaa ccgatccgat cgccgatgcg ggcttcgatg
     2101 aaggtcgcgg ctttgctgtc ccgtccgtga gtcagcgact gctgggtcag ttgttcgatg
     2161 tcgagggcta tcccgcgcgc acggaggatt ccggtcagcg ccgagacggg gccgttgcca
     2221 acaccggttg cggtgatgcg ctcgccgcca atgcgcaggg tcgcgacgag atgatcctcg
     2281 ccagaagcgg cctgcagcga ccactcctcg agggcgactg gtccttccag gttgacatag
     2341 gtctcgagga agaggtcgtg gagccgggag gcggcgatct cctcacccgt cgtgtcggtc
     2401 tccgcctgca ccacccgcga gaagttgacc tgaagcgcgc gcggcatctc gagaccgagc
     2461 tcctgctcga cgacccaggc gatgccgcct ttcccggatt ggctgttgac gcggatcacc
     2521 gcctcgtaat cgcgtccgac atcggccggg tcgatcggaa gatacgggat atcccacggt
     2581 gcggcggacg acgcgacgcc gagctctgcc gcgcgtgtct cgtgatgggc gaaacccttc
     2641 cggatcgcat cctggtgcgt tcccgagaac gcggtgtaga cgagttcgcc ggcgtaggga
     2701 tggcgttcat ggatgccgat ccggttgcac gcttccacgg tgcgctggat cgcgtcgata
     2761 tccgagaagt cgagctgggg atcgattccc tcggcgtaga ggttcaacgc gagcgtgacg
     2821 agatcgacat tgccggtgcg ctcgccgttt ccgaagaggc agccctcgat ccgttccgct
     2881 cccgccatca ttgccagttc ggcgcaggcg acaccggtgc cccgatcgtt gtggggatga
     2941 atcgagagga tcacgctctc ccggcggtcg aggttgcgat gcatgtactc gatctggtcg
     3001 gcgtagacat tcggggtcgc gatctcgacc gtcgccggga gattgaggat gaccggtcgt
     3061 tccggcgcgg catcccagag atcggtcatc cgcttgcaga cctcgaggat gtaatctggt
     3121 tcggtctggt tgaagacttc cg
//
LOCUS       DASTMD010000906         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2319416, whole genome shotgun sequence.
ACCESSION   DASTMD010000906 DASTMD010000000
VERSION     DASTMD010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2319416"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(440..1246)
                     /locus_tag="VFQ54_13420"
     CDS             complement(440..1246)
                     /locus_tag="VFQ54_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166041.1"
                     /translation="MKRFASTYRFSGFAAPRTFGTRAVTCLLTIAMVTACMWSGGGFV
                     TSGAEAAAAATCFPSNNPFKVRISWTVVERDLAAPPASAPVVADVTAVITQVYRPDLT
                     MDYTALDRLSTPEYKKMTGLDCEGVAAPPAPGTPAPTADRPRLDQVRQVDPTTVVAFL
                     SVPGHAAGPAPGATPAKVGVNGEGTPYPGTVVTDAVYLVVFVQRDGHWTIDFLSGAQV
                     NFSDVVSKDPQLRANGGTYSVEAIDLRDTGMPALPADCILLATPEATPGS"
     gene            1765..>2615
                     /locus_tag="VFQ54_13425"
     CDS             1765..>2615
                     /locus_tag="VFQ54_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEU0166042.1"
                     /translation="MSVSGWLHHQRLLKAVDFLLLRDSTGIAQIIVEDTDLRSMTQAL
                     PHESVLAIEATVVASSQAPNGIELHAPRVTVIGRSTEEPPFELFRPETGARLPTLLDH
                     AAIALRHPARAAAQRLLATSLHGFRATMRSHRFTEISTPKILGAAPEGGANVFPVDYF
                     GTTAYLAQSPQLYKQIMVGALERVFETAPAFRAEPHDTTRHLSQFLSLDAEMGFITDH
                     QDVMQMATAAVRGMLDEIATETRSIATLELALPEVPSAIPQVHFADALALVGEATSED
                     LSGEPDLA"
BASE COUNT          481 a          881 c          793 g          460 t
ORIGIN      
        1 agcttgtctt gagcttgtcg aatggatccc cccactcaga ccgcgctccc gtggcgcgcc
       61 atcctgtagc cagacaggaa cgtcccatgg atgccgctga ccgatcatca ccattggacg
      121 aaccgtagac gcgatgcttg tcatcgcccg gatcggctcc gccgatccgt gtccgtggcg
      181 gaatgacgcg gaaccatcag cgcgttgtgc ggagctttcc gtatgcggcg agagacgaac
      241 tctcgcccta caaacccgcg caattgtgag caccgtcggt gagaaaattt cgttggaggc
      301 tgcctccggc ggcgggacca aaccgcaggt cgatccacga tcaggaacgt ccgaacaacg
      361 cacgcacgcc ctgtgaaaag acatgaagga agcaggctcc ccgatgggag cctgcttcct
      421 tccccggttc tcgatagaac tagctgcctg gagtagcctc cggggtggcc agcaagatgc
      481 agtccgctgg aagagcaggc atgcccgtat cacgcagatc gattgcctcg accgagtagg
      541 tccccccatt cgcccgcaac tgcggatcct tcgacacgac gtcggagaag ttcacttgcg
      601 ccccggagag aaagtcgatc gtccaatgac cgtcacgctg gacgaagaca acaagataaa
      661 cggcatccgt gacgacggtt cccggatagg gagttccctc gccgttcacc ccgaccttgg
      721 ccggtgtcgc tcccggcgcc gggccggctg cgtgacccgg caccgagaga aatgcgacga
      781 cggtcgtcgg atccacctgc cgcacctggt cgaggcgcgg ccgatccgca gtcggagcgg
      841 gtgttcccgg cgccggggga gccgccacgc cctcgcaatc cagaccggtc atcttcttgt
      901 actcaggtgt ggaaagccgg tcgagagcgg tgtagtccat cgtcaggtcc ggccgataga
      961 cctgcgtgat cactgccgtc acgtctgcga ccaccggcgc cgatgcgggc ggtgcggcaa
     1021 ggtcgcgctc gacgacggtc caggagatac gcaccttgaa gggattgttg cttgggaagc
     1081 acgtagccgc ggcggcggct tcggcgccgc tcgtcacgaa tccgccgccg gaccacatgc
     1141 aagcggtgac catcgcgatc gtgagcaggc aggtcacggc acgcgtaccg aatgtgcgcg
     1201 gtgcggcaaa gccggaaaag cgatacgtcg atgcgaatct cttcatcctg ggtcccttcg
     1261 ttgatcccga acgacgttag tccctattcg tttttaaatc gttccttata tcctatcacg
     1321 ttgcgcaatc gcctctctcc ggggtacggt tgcgccaacg cgttgacgga gacgagtagc
     1381 aacgacccct gatcccaagc gagtcacgaa cggtggaagc gtgacgatca tgccgcggcg
     1441 aagaccctcc cgagtgcgtg actgaacggc gatcccctgg tcggcaccaa gaccgacccc
     1501 gacgggaaac gcagataggt cgcgccggcc cacctccgtt accgggttcc agaaggtcga
     1561 tccatcgaga tcgacgaatt gcggtggcac cacgagacgc tcccctcgtc cgcatggcgg
     1621 agggagtgtc tggttgtttt cggaggtgac accgtgtcct ccccttccct ttcccctcgt
     1681 tccgtttcgc cgcccgtcgt tctccacgag gcatcccctc cccgcacgcg tatcgccgac
     1741 ctcggcctgc acgtcggaga gaccgtgtcc gtgagcggct ggctgcatca tcagcgactc
     1801 ctgaaggcag tcgactttct cctcttgcgg gattccaccg gcatcgccca gatcatcgtc
     1861 gaggacacgg atctccgttc catgacgcag gcgttgccgc acgaatccgt cctcgcgatc
     1921 gaggcgaccg tcgtcgcgtc gagtcaggca ccgaacggaa tcgagctgca tgcgccacgc
     1981 gtgaccgtga tcgggagatc gacggaggag ccaccctttg agctcttccg gcccgagacc
     2041 ggtgcgcggc tgcctacgct cctcgatcat gctgcaatcg cacttcgcca tccggcacgc
     2101 gcggcggcac agcggctgct cgcgacaagc ctccacggtt tccgcgcgac gatgcgatcg
     2161 catcgcttca ccgagatctc gacgccgaag atccttggtg cggcgccgga gggtggcgcg
     2221 aacgtcttcc ccgtcgacta cttcgggaca acggcctatc tcgcgcagag cccgcaactc
     2281 tacaagcaga tcatggtcgg cgcgctcgag cgcgtcttcg agacggcgcc cgccttccgc
     2341 gcggaaccgc acgacacgac gcgtcacctg agtcagttcc tctcgctcga tgccgagatg
     2401 ggattcatca ccgaccatca ggacgtgatg cagatggcga ccgcggcggt acgcggcatg
     2461 ctcgacgaga tcgcaaccga aacgcggtcg atcgcgacgc tcgaactcgc cttgccggag
     2521 gttccatcgg cgattccgca ggttcacttc gccgacgcgc tcgccctcgt cggcgaggcg
     2581 acgtccgagg acctctccgg ggaacccgac ctcgc
//
LOCUS       DASTMD010000907         1820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2677470, whole genome shotgun sequence.
ACCESSION   DASTMD010000907 DASTMD010000000
VERSION     DASTMD010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2677470"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VFQ54_13430"
     CDS             <1..662
                     /locus_tag="VFQ54_13430"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0166043.1"
                     /translation="AMIGQSMINVKSGGRGRLSTFVAGMVLLFLVVALGDWVAKIPMG
                     ALIAVMVMTSIATFDWRSFRQLRVHPKTSSFVMIAVVIVVVATNNLAIGVGVGVLLSG
                     VFFAWKVSQLVTITSELATGGTSRTYHVGGQIFFATADALANGFDVREGVERVRIDLT
                     DAHFWDISAVGALDKVVMRFRREGVEVEIVGMNEASARFADRISPREASDETLDLAPG
                     H"
     gene            complement(742..1011)
                     /locus_tag="VFQ54_13435"
     CDS             complement(742..1011)
                     /locus_tag="VFQ54_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166044.1"
                     /translation="MAKNKGNGGGRVGAVRGRTQFQLPDGHYAKRDRWTGEILAIKAD
                     LKPFKGIVRERPEELPIRVLESDAGPAGSRESRVTEFLEPLSRAA"
     gene            1173..1718
                     /locus_tag="VFQ54_13440"
     CDS             1173..1718
                     /locus_tag="VFQ54_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEU0166045.1"
                     /translation="MQTIVFTLRFDRDSERAVRAIRERVNAAGIKLPGVTVHRPHITV
                     AAYDVEDVDANISALTGAIRTVPPFELGLDYLGVFPERRVAFLAPRPIPELRSLHQRI
                     VDHFSGPDFPPMKWELLLPGRWVPHCTIAADLSPTDLTKVIDICHRSWTPVSGRVEAV
                     GVLLPPAVEDSCEVRLRGDEV"
BASE COUNT          302 a          595 c          542 g          381 t
ORIGIN      
        1 gcgcgatgat cgggcagtcg atgatcaacg tgaagtccgg cggtcgcggt cgcctctcga
       61 ccttcgtggc cggaatggtg ctgctcttcc tcgtcgtcgc gttgggtgat tgggtcgcga
      121 agatcccgat gggcgcgctg atcgcggtga tggtgatgac gtcgatcgcg accttcgact
      181 ggcgatcgtt ccggcagtta cgcgtccacc cgaagacatc cagcttcgtg atgatcgcgg
      241 tcgtcatcgt cgtcgtcgcg acgaacaatc tcgcgatcgg cgtcggggtc ggcgtcctcc
      301 tgagcggcgt cttcttcgcc tggaaggtaa gccagcttgt gacgatcacc tccgagctcg
      361 caacgggcgg gacatctcga acctatcacg tcggcggcca gatcttcttc gcgacggcgg
      421 atgcgctcgc gaacggcttc gacgttcgcg agggtgtcga gcgcgttcgg atcgacctca
      481 ccgatgcgca tttctgggac atctccgcgg tcggcgctct ggacaaggtc gtgatgcgat
      541 tccgacgcga gggcgtcgaa gtcgagatcg tcggcatgaa cgaggcgagc gcccgctttg
      601 ccgaccggat ttcgccccgg gaggcgagcg acgagacgct cgatcttgct cccggacact
      661 agaccattcc cgccgcccgc aaaggaaaag gcgcccggtc gaacgaccag acgcctcatc
      721 ctcgcaacaa acggttcttc gtcacgccgc ccgtgaaagc ggctccagaa attcggtgac
      781 acgcgattcg cggcttcccg caggacccgc gtcgctctcc aaaacccgaa ttggcaattc
      841 ctcgggccgc tcccggacga ttcccttgaa cggcttcagg tcggccttga tcgcgagaat
      901 ctcgcctgtc cagcggtcac gcttcgcgta atgaccatcc ggcagctgga actgcgtcct
      961 cccgcggacg gcgccgaccc ggccaccccc atttcccttg ttcttcgcca tgtattaccg
     1021 ctttctcaga cactttcgcc ggtttcggct ccccaatatt gtttttccat tgtaccattt
     1081 attctatatt atgtctacaa atgacgtggt cacccagatc gcgagatatt attgctggct
     1141 attggcttga cccgcgaacc ggaggtcacc tggtgcagac gatcgtgttc acgctcagat
     1201 tcgaccggga ctcggagcgt gcggtccgcg ccatccgaga gcgcgtcaat gccgccggga
     1261 tcaagctccc cggagtcacc gtccaccgcc cacatatcac ggtcgccgcc tatgacgtcg
     1321 aggacgtgga cgcgaatatt tctgcgctca cgggggccat acgcacggtc ccgccattcg
     1381 agttaggtct cgattaccta ggcgtctttc cggaacggcg ggtcgcgttc ctcgcgccgc
     1441 gaccaattcc tgaactccgg tcgctgcacc agcggatcgt cgatcatttc tcgggaccgg
     1501 atttccctcc catgaaatgg gagctccttt taccgggcag gtgggttccc cattgcacga
     1561 tcgccgccga cctctccccc actgatctga ctaaggtgat cgacatctgc caccgctcgt
     1621 ggacgccggt ttccggccgg gtcgaagccg tcggtgtgct tctcccgcct gccgtggagg
     1681 actcctgcga agtacggctt cgcggagacg aggtctgagc ccctgcaggc tctgcggccc
     1741 gggacgcatt ctcgggtggg ctcggggcag gctcccgaac gaccgtcgta tcgatgatcg
     1801 tgtacgggaa tcgccacggt
//
LOCUS       DASTMD010000908         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2854568, whole genome shotgun sequence.
ACCESSION   DASTMD010000908 DASTMD010000000
VERSION     DASTMD010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2854568"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFQ54_13445"
     CDS             <1..237
                     /locus_tag="VFQ54_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0166046.1"
                     /translation="LDDKVVPPNQAQDMADAVRAKGLPVAHFTYEGEGHGFRKAENIQ
                     LTAERELSFYAQLFDFTPADDIPAIAIDNLTTAS"
     gene            complement(447..1271)
                     /locus_tag="VFQ54_13450"
     CDS             complement(447..1271)
                     /locus_tag="VFQ54_13450"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU0166047.1"
                     /translation="MRTLSTLNRRAFLHRAGALTGGGAIAIAGFSAISPTTGFAQSAS
                     AGIAFEPGDQVDVITDSLNLRSDASISASVVGTYPNGTVATVTGSPVSADGFDWYPVT
                     VVSDGASGWFAGLYIDKSVAGSHGNGFTVVDGPVNLRASYGLSSQVISMYQTGWTGYA
                     FPSEPVNADGYTWAFVQMNDGTRGWLATDFVAFDPATGDPTGENFTVSAGPLNLRQEP
                     SLSAPILGSYPVGARGFGDESSETAADGYDWINVKLAAANGDPVRGWFALNSITLD"
     gene            complement(1578..>2505)
                     /locus_tag="VFQ54_13455"
     CDS             complement(1578..>2505)
                     /locus_tag="VFQ54_13455"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU0166048.1"
                     /translation="LNRAPAAWQRLWLVPRWLISNYNRVQAGDLAASVAYHALIAILP
                     MFFLLITVAGFFLRNDQEVLARAYEIVNQVFPSGGIGPDAFAQAFDARQSNGWIGVIS
                     VIGFTWAGTGLVSSLARSMNRIYGVRGSGYFSEKRRGIVVILIFALFFVISSIVPIVT
                     TFFVSQELPSILQRWVLASRSGQAIAYGISFASAMIMFAVIYRIVPNAGQRVSNIWPG
                     TIVASALFLLVTQVFPIYIGLIGGVGRNGQIFGFVLLVVASLYAFAQVFLFGAFLNAE
                     FLRVLRRRRHSRLHDHAESLESDSWENPISRS"
BASE COUNT          506 a          822 c          786 g          391 t
ORIGIN      
        1 ctcgacgaca aggtggtgcc gccgaaccag gcccaggaca tggcggacgc cgtgcgcgcg
       61 aagggacttc cggtcgccca cttcacctac gagggcgagg gtcacggctt ccggaaggcg
      121 gagaatatcc agctcacggc ggagcgcgaa ctctccttct acgcgcagct cttcgacttc
      181 acgccggccg atgacatccc cgcgatcgcc atcgacaacc tcacgaccgc atcgtagatc
      241 cagccgcagc acacgacaga gggcgccggc gatgtccggc gccctctgtg cgctcaggta
      301 tcgatggtgc gaacaagaac ggctcctgtc tatcgcctgc cccaggcgac agccgacttc
      361 ttccgatacg cttcatttcc ggtgtcaatg tccgaccccg gccctcacca gccagaatcg
      421 cgtgacggaa cagccccggt tcgacgtcag tccagcgtga tgctgttgag cgcgaaccag
      481 ccccggaccg ggtcgccatt cgcggcggcg agcttcacgt tgatccagtc gtatccatcc
      541 gccgcggtct cggaactctc gtcgccgaac ccccgcgcgc cgacaggata gctacccaga
      601 atcggggcgc tcaggctcgg ctcctggcgc aggttgagcg gtcccgccga gaccgtgaag
      661 ttctccccgg ttggatcacc cgttgccgga tcgaacgcga cgaagtcggt tgcgagccac
      721 ccgcgcgtgc cgtcattcat ctggacgaac gcccacgtgt agccgtccgc gttcaccggc
      781 tcggacggga aggcgtatcc cgtccacccc gtctggtaca tcgagatgac ctgcgaggag
      841 agcccataac tcgcgcgaag attgacgggg ccatcaacca cggtgaaacc gttgccatgg
      901 ctcccggcca ccgacttgtc gatatagagc ccggcaaacc acccgctcgc gccgtcgctg
      961 acgacggtca ccggatacca gtcgaagcca tcggccgata ccgggctccc ggtcaccgtc
     1021 gccactgtgc cgttcggata ggtcccgacg accgatgccg atatggatgc atccgatctc
     1081 aggttcaggg aatcagtaat cacgtccacc tgatcgcctg gctcgaatgc gatcccggcg
     1141 gatgcactct gcgcgaatcc cgtggttggc gagatcgcgg agaatccggc gatcgcgatt
     1201 gccccgcctc cggtcaaagc accagcacgg tgcaggaacg cacgacggtt gagtgtcgat
     1261 aacgtacgca tagcctcact gcctcttgtc attcgcgatc gggggtggcg gtgatcgggc
     1321 gccgcccgcc gatcgacacg gtatggaaga ggacgtcctg cttttgtaac gcatcggata
     1381 tcggacggtt tcgccgcggc aatctcaccg gaaaccaccc gggtttacgt cgagtaccag
     1441 cagacgagcc cgtcaaggat cgtcgatgcg tcgtcgacgt ccacgaaccg gagcgaccgg
     1501 accgggttgt gtcgacgcgc cccttgccgc agataccatg ccggaagggc agcttcgtgg
     1561 ctcaggatcg cgccggctca cgaccgggag atcgggttct cccaggaatc ggattcgagc
     1621 gactccgcat gatcgtgcaa gcgcgagtga cgacggcgac ggagaacccg caggaattcg
     1681 gcattgagga atgcgccgaa cagaaagacc tgggcgaagg catagagcga ggcaacgacg
     1741 aggagaacga agccgaagat ctggccattg cggccaacgc caccgatgag tccgatgtag
     1801 atcgggaaga cctgggtcac gagcaggaag agcgccgagg cgacgatcgt gcccggccag
     1861 atgttggaga cccgctgccc ggcgttcggc acgatccggt agatcacggc gaacatgatc
     1921 atcgccgagg cgaaggagat gccgtaggcg atcgcctgac cgctgcgact cgcgagcacc
     1981 caccgctgga gaatcgacgg cagctcctgg ctcacgaaga aggtcgtcac gatcgggacg
     2041 atactcgaga tcacgaagaa gagggcaaag atcaggatca ccacgatccc gcgccgcttc
     2101 tcgctgaaat agccgcttcc gcgcacgccg tagatgcggt tcatcgagcg cgcgagggag
     2161 ctgaccagcc ccgtcccggc ccaggtgaat ccgatcacac tgatcacacc gatccagccg
     2221 ttgctctgcc gggcgtcgaa cgcctgcgcg aacgcatcgg ggccgatgcc gccggacgga
     2281 aagacctgat tgacgatctc gtaggcgcga gccagcactt cctggtcgtt gcggaggaag
     2341 aatccggcga cggtgatcag gaggaagaac atcggcagga tcgcgatcag cgcgtgatac
     2401 gcgacggacg ccgcgagatc gcccgcctgc acccggttgt agttcgagat caaccagcgc
     2461 ggcaccaacc agagtcgctg ccaggccgcc ggcgcccggt tgagc
//
LOCUS       DASTMD010000909         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2764066, whole genome shotgun sequence.
ACCESSION   DASTMD010000909 DASTMD010000000
VERSION     DASTMD010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2764066"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..883)
                     /locus_tag="VFQ54_13460"
     CDS             complement(197..883)
                     /locus_tag="VFQ54_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0166049.1"
                     /translation="MDDSANPKRRTAEERREEIIAAAIVEFATFGYHGGSTERIAEAA
                     GISQPYTLRLFKTKKAIFLAALHRVCDNITATWATTNEHLDRLGRTPPPSPSERLESM
                     GISFMTLVKEVNDLRLVLQAFSSAEDDDIRTASHARLKQMFDWVRETTGAPFDSVREF
                     FAYGMMLMVAASIRAADDVATEPWARAFLAIPTDPDGNLVDRLSSPVPLLLKPNPFWS
                     NLDQPDSGPD"
     gene            1182..>2211
                     /locus_tag="VFQ54_13465"
     CDS             1182..>2211
                     /locus_tag="VFQ54_13465"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0166050.1"
                     /translation="MVSAAIDGSARVFLGGGVGEESISAPPPDIQPLAWPLERPGYTS
                     LAAAVQGEASRWNVPGMSVAVLHDGEIHEVATGVTSILTRQPMTTDTISQIGSISKVF
                     TTTVVMQLVDEGKIDLNVPITTYIPELPLADENARANLTMAHLLSHSGGFEGDRFLDY
                     GRGDDATTKSMLAMDTLKQWFQPGELFSYCNVAFYLIALVIERVTGEVGEKVMQKRLF
                     DPLKLDGLTYFADDAITWPHAIGHYLKDRATGLEIARPYSFPRHVNLCGMIIATPRHL
                     IRFAQMHMNDGELDGVRIISQEAAQLMRTPRINAGSEYRNYGQGWCVWDYPDFTAVEH
                     GGATMGLRA"
BASE COUNT          414 a          641 c          733 g          423 t
ORIGIN      
        1 gggcctgagc cgacgatacg atcgttgtgt ccgagaggtc gggatgacga ggcggtcgcg
       61 ttcacaggat gactccattc atgttgcaga acagttgtga gtgatcacaa cctagggaaa
      121 atgaggctcg aactggtggt gcgcggttca tatcagatcc tcggtggggt gcatagatgt
      181 tcgcgagcgg gtggcgtcag tcggggccgg agtcgggctg gtcgaggttc gaccagaatg
      241 gatttggctt gagcagcaat gggaccggag agctgaggcg gtcgacgagg ttgccgtccg
      301 gatcggtcgg gattgcgagg aaggcgcgcg cccagggctc ggtggcgacg tcatcggcgg
      361 cccggatcga ggccgcaacc atcagcatca ttccataggc gaagaactcg cgaacgctgt
      421 cgaagggggc gccggttgtc tcccggaccc agtcgaacat ctgcttgagc cgggcgtggc
      481 tggccgtgcg gatgtcgtcg tcctcagcgg aactgaacgc ctggaggacg agccggagat
      541 cgttcacctc cttcacgagc gtcatgaacg aaatccccat cgactcgagg cgctcggatg
      601 gcgatggggg cggcgtacgc cccagccggt cgaggtgctc gttcgtcgtg gcccaggtgg
      661 cggtaatgtt gtcgcaaacc cggtgaaggg cggcaagaaa gatggccttc ttcgtcttga
      721 agaggcgcag cgtgtatggc tgcgaaatgc ccgccgcttc ggcgatccgc tcggtggaac
      781 caccatggta gccgaaggtc gcgaactcga cgatcgcggc ggcgatgatc tcctcgcgtc
      841 gttcctcggc agttcttcgc ttgggatttg cgctgtcatc cacggcattc gctcacatcg
      901 gatggttggt cggatcgggt gttccgcatg gatcgaaggg gaccggattg tctgaaacgc
      961 ctgaactcgt atggttgtga gtctacacta ccatacgaga agtgcaagtc tttcggtcgc
     1021 aattcaggcg caaatctgtg gctgcggata ctatggcgga cacaatcgac acgcatggcc
     1081 acggcatgtc cgatcgcgaa gcacgcgtgt gtgcgatcgg acgagatcgc ggtgcggttg
     1141 tcgttggcat cgcagtgctt cggaaggaga catcgcacca gatggtcagt gcagcaatcg
     1201 atggttcagc ccgggtcttc ctcggcggcg gcgttggcga ggaaagcatt tccgctccgc
     1261 cgccagatat ccagccgctg gcctggccgc tcgagcgtcc cgggtacacc agtctcgccg
     1321 ccgcggttca gggtgaagcg tcgcgttgga acgtccccgg catgagtgtc gccgttctcc
     1381 acgatggcga gatccatgag gtcgcgacgg gggtgaccag catcctcacc agacagccga
     1441 tgacgaccga tacgatctcc cagatcggat cgatctcgaa ggtcttcacg acgaccgtcg
     1501 tcatgcagct cgtggatgaa ggcaagatcg atctcaacgt cccgattacg acctacatcc
     1561 cggagttgcc actcgccgac gagaacgcgc gtgcgaacct gacgatggcg catctcctct
     1621 cccacagcgg cggcttcgaa ggcgatcgct tcctcgacta cgggcgcggt gacgatgcga
     1681 ccacgaagtc gatgctggcg atggacacgc tgaagcagtg gttccagcca ggcgagctct
     1741 tctcctactg caatgtcgcc ttctatctga tcgcactcgt gatcgagcgg gtgacgggcg
     1801 aggtcggcga gaaggtgatg cagaagcggc tcttcgatcc gctcaagctc gacggcctga
     1861 cctacttcgc cgatgacgcg atcacctggc cacacgcgat cggtcattac ctgaaggatc
     1921 gcgcaacggg actcgagatc gcgcgtccct attcgttccc gcgccacgtc aacctctgcg
     1981 gaatgatcat cgccacgccg cgccacctga tccgcttcgc ccagatgcac atgaacgatg
     2041 gcgagctcga tggggtgcgc atcatctcgc aggaggcggc gcagctgatg cgtacgccgc
     2101 ggatcaatgc cggttccgaa taccggaact acggccaggg ctggtgcgtc tgggactacc
     2161 cggacttcac ggcggtcgag catggtggag cgacgatggg gcttcgtgcc c
//
LOCUS       DASTMD010000910         9323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1783054, whole genome shotgun sequence.
ACCESSION   DASTMD010000910 DASTMD010000000
VERSION     DASTMD010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783054"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..1228
                     /locus_tag="VFQ54_13470"
     CDS             272..1228
                     /locus_tag="VFQ54_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166051.1"
                     /translation="MTDPTQFIAPPRTEYDYSPLDLAPPGQRRRRQLVVGIVGILAVI
                     VIAAAVVFGYILLKGGDDDNKGPSQQDQLAAAQTVVAQNAARTGTPETDEVAQTDPTV
                     TTAASGADGATDPTKPSTTTTGQAGASNPTPKPTSTTVAGPSLGSLQKLLPAANLMPQ
                     GLDTPTDNSKDEKAVVDALGGSRTAQQNLDKWGWSGNAAIEYTASDSSALVPDAVTDV
                     SVSIHGFSTAGDAQDALTFFSDVLLNQSSDGTWTEATDPKVGSKSRMMTQSIDNGDGT
                     TTTNVALYVSDGRVLYRIGGSASNGGDPTDAIIQLATTLLGS"
     gene            1329..1622
                     /locus_tag="VFQ54_13475"
     CDS             1329..1622
                     /locus_tag="VFQ54_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166052.1"
                     /translation="MFNRDNAIGMLLLGFCVLVGGYMVYVIATGKRVSIHINGTTGVV
                     LTLVFIGLIIYGIAKSGTFSRFGGGNRGGRQWPDPQTGQSGRRRSLWDRLRGR"
     gene            1655..2356
                     /locus_tag="VFQ54_13480"
     CDS             1655..2356
                     /locus_tag="VFQ54_13480"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEU0166053.1"
                     /translation="MTTDIEPKVHDWRIAPREYARHEARRGRRHAFDHLDPERTALVV
                     IDMVAFFVAENALCLGIVPNINRIATTLRDAGGTIAWVLPRVDASPTAWASEFYGPDI
                     AALYAASGGEGSMHDRLWGGLASRDDDLWVEKSASSAFFPGRSPLPELLAAKGIDTVL
                     ITGTVTNVCCESSARDASTLGYRVIMVADGNAGVRDEIHNATLNTIYRSFGDVRPTSE
                     VIDLVAAADSRSRGD"
     gene            complement(2469..4295)
                     /locus_tag="VFQ54_13485"
     CDS             complement(2469..4295)
                     /locus_tag="VFQ54_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166054.1"
                     /translation="MLTLPARSHRHRLGARHMLGLPVALILLVNLIGISTVSAQSAPA
                     TAIASPAASPGATPEIALTSVIAPATAAGTDQLVLTDTVVTSGTATWKVTTPLTGQIT
                     FNSVSCDPTGTTCDSTYDPTTNAVTTTGTLTAGAPRGQATIRIVAALIGGAKAGTLTI
                     AGCATTGDATTCGDAGTDGANATLTIATPAPVGVSTAVTAGSASESAATPATGSTTSE
                     ATPAPAAAGATTTETAATPASGTTTSAIPASGETPATPAESTTAETAATPAPGTTSSV
                     PASGEAAATPAASVTTGEAAASPVAGSAATPASGNAGNEIALSSVISPATASGSDQVI
                     VSQNLVATGSGSWTVKTPLTGNVVYRGVSCDTAGTTCDSTYDATTNTLVTTGTIDPSA
                     PRGQATIRIIAAVVGGAQAANVTINSCASFGGVACGAAGTDSSTAFLKVVRTANEVIP
                     ATSGSGASTSGTTSTSGGTTTSGSNATPAAGGTTSSTSGATPTSGAASSTGSSATPAS
                     GAASTGSNATSASGGATTTGSATSSPATTVAPTTAPAPTATTAPAAPVATATPAPTEE
                     AGSGDDNSNRNLTIIIIVAAVLIGGLGLGALRNIRAGGNPFR"
     gene            4767..5624
                     /locus_tag="VFQ54_13490"
     CDS             4767..5624
                     /locus_tag="VFQ54_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166055.1"
                     /translation="MTDDPNQRPRPASDDEPEESNASGDVDAADAPDDAGSSNPDDTQ
                     DARSGRVRLGGRDANGRPFSLYLVLGLGVAALLVLLAIIYFSSVNNNNPEQPICTTID
                     PASAQQSVLQGDVKRVVVNYDKGVASATDPSWGPVLSRIDYTNGECGNLPQGIEQRNA
                     ITLVLGTIYLYNEITSEPQVEIKLAGSNSLSPALFETPTSAPTDTPVPTPTSSVAAVT
                     SPVVMTPTPTAPAAATPPSPTGTPEPTATVPIVAEPGTPRSGTPVSSGSPAAGASPEV
                     PTATATPTP"
     gene            complement(5703..6770)
                     /locus_tag="VFQ54_13495"
     CDS             complement(5703..6770)
                     /locus_tag="VFQ54_13495"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0166056.1"
                     /translation="MQSFWAMLVANMKMSARNRAAFFWNLLFPALFILIFPALFSSGI
                     GGTPSIGITGADTPLRQQVTQALKDNGGFDLHEGDQGKEMASLKDGDRSLVIVFPAGE
                     LSDSSPIQLLTSSQDDPSGQIALGSVRSALLDILGQGRGIAVTQDKISTLDTTFVDFF
                     VPGILAMSIMNAGMIGLATNFVSYRERGILRRIKVTPLPLWKFILTRIIASLAINLVA
                     SAVLIVMARLVWGLHIRGNWFLILGILILGGLAFLGIGYAIAAVSRNIETAAAYGNAL
                     TFPMLFLSGVFISVSSMPGWLQPIARILPLRYLVDALREPMEYGKGVSDVWIPTLVLV
                     AFFAVSFAFAVRFFKWDATPR"
     gene            complement(6842..7894)
                     /locus_tag="VFQ54_13500"
     CDS             complement(6842..7894)
                     /locus_tag="VFQ54_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0166057.1"
                     /translation="MTSISSSSTTPTATAPPIISIRGLTKRYKGFTAVDNIDLEVRTG
                     EIFGILGPNGAGKTSTLEMIEGLRTHDSGTITVAGLDAVKQSDDVRKVIGVQLQSTSL
                     FPYLTAAELIELFGHMYESDNPKERVGTLLRLVGLEEKGKARAEEMSGGQQQRLSIAL
                     ALVNQPQVTFLDEPTTGLDPLARRSLWETILGVRDAGTTVVLTTHYMEEAEVLCDRIA
                     IMDGGHVIACDTPAGLIRDLDLEATVSATISGASEMLDESTLASIPGVTSSSLSTSET
                     PPRLKLQTRDAQATIIGLLTHASNHGITLGELSSTRASLEDVFLSLTGRTYVQPEAPP
                     EDAGTGTGRRRRRRRG"
     gene            complement(8015..8371)
                     /locus_tag="VFQ54_13505"
     CDS             complement(8015..8371)
                     /locus_tag="VFQ54_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166058.1"
                     /translation="MASPSVLEITCHGTPRVELTRYTYQLSRGASSDMRSDVVAYHWT
                     PFDPQAKQTAPHLHIGSSFIERDHRRAPWSFHKAHLTTGIVTVPMFVRMLVEEFDISP
                     VNDRWEHLLASAERAT"
     gene            complement(8358..8612)
                     /locus_tag="VFQ54_13510"
     CDS             complement(8358..8612)
                     /locus_tag="VFQ54_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166059.1"
                     /translation="MALKKSSPPYTFTSEEIAELEAALPPVMEMTDEEYRAIFEDRAQ
                     SLFGVGADEFTRRYRAGEYDLDDYRVADLAFFIPVRDGES"
     gene            8931..>9323
                     /locus_tag="VFQ54_13515"
     CDS             8931..>9323
                     /locus_tag="VFQ54_13515"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0166060.1"
                     /translation="MDPVSTNDLPRLDVSGEGDDTVRWTAAFVAYGDHGADQTTTLVF
                     EIEPGGRLGWHTDQTEETQYIIAGTGELQRDDATLPVGPGSVFVLPTNVRHDLVNTGT
                     ETLRAVGFFGAAMFTQRFDNVMLPPKTHI"
BASE COUNT         1616 a         2936 c         3126 g         1645 t
ORIGIN      
        1 cgttccccgt tcgtcgatgt ggtgatgatg gactcccgtc ctcgtgatgg gatgccaccg
       61 ttgtggctga ttcgctcgcc gcaacgctgc ggagtgcggc atcgcgacgt cggcacacaa
      121 gacggaaagg atccgggaac aggaccccat ggagccacga ccacaacgac cacgaccaac
      181 accaggccaa ccggcgggcg gatcatctcc cgtcgctggt ggatcatccg ccgcaggagc
      241 tccagccgcc cagggttttc acgatggtgg gatgactgac cccacccagt tcatcgcccc
      301 cccacgcacc gaatatgact acagcccgct cgatctcgca cccccggggc agcgacgtcg
      361 acgccagctc gtggtcggga tcgtgggcat cctcgccgtc atcgtgatcg ccgccgcggt
      421 ggtcttcggg tacatcctcc tcaagggagg ggatgacgac aacaagggcc cgtcgcagca
      481 ggaccagctc gccgccgccc agaccgtcgt cgcccagaat gccgccagga ccggcacgcc
      541 ggagaccgac gaggtcgccc agacggaccc gaccgtgacg actgccgcct ccggcgccga
      601 tggggcgacg gacccgacga agccttcgac caccaccacg gggcaggcgg gagcgagcaa
      661 tccgaccccg aagccgacat cgacgaccgt ggctgggcca tcgcttggga gcctgcagaa
      721 gctcctcccc gccgcgaacc tgatgccaca ggggctcgat accccaaccg ataattccaa
      781 ggatgagaag gccgttgtcg acgcgctcgg tggcagccgc acggcccagc agaatctcga
      841 caagtggggg tggagcggga acgcggcgat cgagtacacc gcgtccgact cctcggcgtt
      901 ggtgccggac gccgtgaccg atgtttccgt cagtatccac ggcttcagca cggcgggaga
      961 tgcgcaggat gcgctcacgt tcttctccga tgtgctgttg aaccagtcga gtgacgggac
     1021 gtggacagag gccactgacc ccaaggtcgg gagcaagtcc cggatgatga cgcaatcgat
     1081 cgacaacggg gatggcacca caacgaccaa cgtcgcactc tacgtgagcg acggtcgtgt
     1141 cctctatcgc atcggtggat ccgcctcgaa tggcggcgat ccgacggatg ccatcatcca
     1201 gcttgcgacg accctgctcg gaagttgaga cacggtccgc gcttttgacg cacaatggaa
     1261 tctacaggtt tatgacgaac ccccggtcat cttccggggg ttcatcgttt tcggacaagg
     1321 aagcgcggat gtttaatcgc gataatgcca tcgggatgct cctcctcgga ttctgtgtgc
     1381 tggtcggcgg atatatggtc tatgtgatcg ccaccggcaa gcgggtgtcg atccacatca
     1441 atggcacgac cggggtcgtc ctgaccctgg tcttcatcgg cctcatcatc tacggcatcg
     1501 ccaagtccgg gacgttcagc cgctttggtg gcggcaatcg tggcggccgg cagtggccgg
     1561 atccgcagac cgggcaatcc ggacggcgcc gcagcctctg ggaccgcctc cgcggccgct
     1621 agcacccgag cccgcgtgga ggagaccgtc gacaatgacc accgatatcg agccgaaggt
     1681 gcacgactgg cggatcgctc cgcgcgagta tgcccgccat gaagcgcgac gcggacgccg
     1741 gcatgccttc gatcatctcg atccagagcg aaccgcgctc gtcgtgatcg atatggttgc
     1801 cttcttcgtc gcggagaacg ccctctgcct gggaatcgtg ccgaacatca accggatcgc
     1861 gacgaccctg cgggacgccg gcggcaccat cgcctgggtg ctgcccaggg tcgacgcgtc
     1921 gccgacggcc tgggcctccg agttctacgg tcctgacatc gcggcgctgt atgcggcctc
     1981 cggaggggaa ggctcgatgc acgatcgcct atggggtggg ctggcgagtc gtgatgacga
     2041 tctctgggtg gagaaatccg cctcgagcgc cttctttccc gggcgctcgc cgcttccgga
     2101 actcctcgcg gcgaagggaa tcgataccgt cctcatcacg ggaacggtga cgaatgtctg
     2161 ctgtgaatcg tcggcgcgcg atgcgagtac gctcggctat cgcgtgatca tggtggccga
     2221 tggcaatgcc ggtgttcgcg acgagatcca caacgccacg ctgaacacga tctatcgctc
     2281 gttcggcgat gtccgtccca cctcggaagt catcgatctg gtcgccgcgg ccgactcccg
     2341 ttcccgcggc gactgagatc caggtcctgc ccgcgtggag ggcgtcgtaa cgaggaaggc
     2401 ccctgagcaa tctgctcagg ggccttactc gtccgattga cggatacgtg tttcgacggg
     2461 tcgcgctgct agcggaacgg attgccgcca gcgcggatgt tgcgcagggc gccgaggccg
     2521 agaccaccga tcaggaccgc cgcgacgatg atgatgatcg tgagattccg gttgctgttg
     2581 tcatcaccag acccggcttc ctcggttggc gccggggtcg ccgttgcgac cggagctgcc
     2641 ggagcggtgg ttgcggtcgg tgctggcgcc gtcgtcggcg cgactgttgt tgcgggagac
     2701 gacgtggcac taccagtggt cgtggctccg ccgcttgccg acgttgcgtt gctcccggtc
     2761 gaagcggcac cacttgccgg cgtcgcgctg cttccggtcg acgaagcggc gccgctcgtt
     2821 ggggttgccc cgctggtgct agaggtcgtg ccccccgcag ccggtgtcgc attgctaccg
     2881 gatgtcgtgg tgccaccgct cgtggatgtg gtgccactcg tggaggcgcc actaccgctg
     2941 gtcgccggga tcacctcgtt ggcagtgcgg acgaccttga ggaaggcggt cgagctatcg
     3001 gtgcccgcgg cgccacaggc gacaccgccg aagctggcgc agctgttgat cgtcacgttt
     3061 gccgcctggg cgccaccaac taccgcggcg atgatgcgga tcgttgcctg gccgcgcggt
     3121 gcgctcggat cgatcgtgcc ggtcgtgacg agggtattcg tcgttgcgtc gtaggtggaa
     3181 tcacaggtcg tgcccgcggt atcgcagctg acaccgcgat agacgacgtt gccggtcagc
     3241 ggcgtcttca cggtccagct tccggacccc gtcgcgacga gattctggga gacgatgacc
     3301 tgatcgctgc cggaggccgt ggccggactg atgacggacg agagggcgat ctcgtttccg
     3361 gcgttcccgg aggcgggagt cgcggccgaa cccgccaccg gggaggcagc cgcctcgccc
     3421 gttgtcacgc tggcagccgg cgtagccgcc gcttcgccgc tcgccgggac actggatgtc
     3481 gtgccgggag ccggagtggc agctgtttcc gccgtcgtgc tctcggcggg agtcgccggg
     3541 gtctcaccgc ttgccgggat ggcggatgtt gtcgtgccgc ttgccggggt cgctgccgtc
     3601 tccgtggtgg tagcgcccgc tgctgccggg gccggggttg cctcggacgt cgtggagccc
     3661 gtggcgggag tcgccgcgct ctcggacgca gatcccgcgg tgacggcagt cgagacgccg
     3721 accggggccg gggtagcgat ggtgagtgtc gcgttcgcgc cgtcggttcc ggcgtcaccg
     3781 catgttgtcg catcgccggt cgttgcgcag ccggcaatgg tgagcgtgcc ggccttggct
     3841 ccgccgatga gcgcagcgac gatgcggatc gttgcctgac cgcgtggcgc accggcggtc
     3901 agcgtgcccg tcgtcgtcac cgcgttggtg gtgggatcgt aggtggaatc gcacgtcgtg
     3961 ccggtggggt cgcagctgac ggagttgaag gtgatctgac ccgtcaacgg ggtcgtgacc
     4021 ttccaggtcg ccgtgccgct ggtgaccacc gtatcggtga gcacgagttg atcggtgccc
     4081 gcggcggttg ccggagcgat gacggatgtg agcgcgattt ccggggtggc accgggtgat
     4141 gcggccggag aggcgatcgc agtcgctggt gcagactgcg ccgagaccgt gctgatcccg
     4201 atcagattca ccaacagaat gagcgcgaca ggcagaccga gcatatgtcg agccccgagg
     4261 cgatggcggt gtgatcgcgc gggaagcgta agcacggcga atactccttg atgaaacggt
     4321 tgactcgcga tggtggctac gcgacccaac cctctgttgc gatcgaacga tccgcggtgt
     4381 tcgccacgtc cacgccactc ccgattcccg ggatcagcat agtgtcacgc ggtcgcgcca
     4441 ccggatgcga gctgccccac cgaagagccg caccgagacg ccgttccttc gcggatggtg
     4501 atcacctgcg tctactcgtt catttttgat tctcgcgtcc ggttcgtcaa tccaaattcc
     4561 ggaagagccc tcacgatcat gcgacgactg tctgatttct ccgcaagagg aagcatactc
     4621 gcccggtgag gaagcgacgt cgccccacac ccggtacact gtgtgcgata gcgtgagggt
     4681 cacagcgggt acgtacgaac gacccttctg gaccaactcc gcgcgcatcg gggccacacg
     4741 caaccttgga ggacaggcga gcagcgatga cggacgatcc gaatcagaga ccacgaccgg
     4801 cgagcgacga tgaaccggaa gaatcgaacg cttccggcga cgtggacgcg gcggacgctc
     4861 ccgacgacgc aggatcgtcg aacccggacg acacgcagga cgcgcgatcg ggtcgtgtgc
     4921 gtcttggcgg ccgtgatgcg aacggccggc cgttctcgct ttacctcgtg ctcggcctcg
     4981 gcgtggcggc gctgctcgtt ctgctggcga tcatctattt ctcctccgtc aataacaaca
     5041 atcccgagca accgatctgc acgacgatcg atcccgcgtc cgcccagcaa tccgtgctcc
     5101 aaggtgatgt gaagcgtgtt gtggtgaact acgacaaggg tgtggcgtcg gcgaccgatc
     5161 cgtcgtgggg gccggtgctt tcccggatcg actacacgaa cggggaatgc ggcaacctgc
     5221 cccaaggaat cgagcagcga aacgcgatca cgctcgtgct gggaacgatc tatctctaca
     5281 acgagatcac atcggagcca caggtcgaga tcaagctcgc cggcagcaac agcctgagcc
     5341 cggcgctctt cgagacgccg acatcggctc cgaccgatac gccggttccc acgccgacat
     5401 cgagcgtcgc cgcggtcacg agtcccgtgg tgatgacgcc gactccgacg gcgccagccg
     5461 ccgcgacgcc accatctccc accgggaccc cggagccaac ggcgacggtt ccgatcgtgg
     5521 cggaaccggg aacgccccgg agcgggactc cggtctcgtc cgggtccccg gccgcgggtg
     5581 catctcccga ggtaccgacg gccaccgcga cccctacgcc gtgacggcgt gcaggagaaa
     5641 caccccgctc ggcgacaccg agcggggtgt ttctcctggt tcgcttaccg gggtgttcct
     5701 ccctaccgcg gagtcgcatc ccacttgaag aagcggacgg caaaggcgaa ggagaccgcg
     5761 aagaaggcca cgaggacgag ggtcgggatc cagacgtccg agacgccctt gccatattcc
     5821 attggttccc gtaacgcatc gaccagatac cggagcggaa ggatgcgggc gatcggttgc
     5881 agccaccccg gcatgctcga tacggagatg aagactcccg agaggaagag catcgggaag
     5941 gtcaacgcgt tgccataggc cgcggccgtc tcgatgtttc gcgagaccgc ggcaatcgca
     6001 tagccgatcc cgaggaacgc gagtccgccg aggatcagga ttcccaggat caggaaccag
     6061 ttgccccgga tgtggagccc ccagacgagc cgcgccatca cgatcaggac ggcgctggcg
     6121 acgaggttga tcgcgagact ggcgatgatt cgcgtcagga tgaacttcca gagggggagc
     6181 ggcgtcacct tgatccggcg caggatgcca cgctcgcgat agctcacgaa gttcgtcgcg
     6241 agaccgatca tccccgcgtt catgatgctc atcgcgagga tgccgggcac gaagaagtcg
     6301 acgaaggtcg tgtcgagcgt cgagatcttg tcctgcgtga cggcgatccc gcggccctgg
     6361 ccgaggatat cgagcagggc gctgcgcacg cttcccagcg cgatctgacc ggaggggtca
     6421 tcctggctgc tggtgaggag ctggatcggt gacgagtcgg aaagctcgcc tgccgggaag
     6481 acgatgacga ggctgcgatc gccatccttc agcgaggcca tctccttgcc ctggtcgccc
     6541 tcgtgcaggt cgaagccgcc attgtccttc agtgcctgcg tgacctgctg ccggagcggc
     6601 gtgtcggcgc cggtgatgcc gatgctcggc gtaccaccga tcccgctgct gaagagcgcc
     6661 gggaagatga ggatgaagag ggcgggaaag agcaggttcc agaagaacgc tgcccggttg
     6721 cgggccgaca tcttcatgtt ggcgacgagc atcgcccaga acgattgcat cagcgggact
     6781 cccggtagga acggtcggag aggtatgttg tcgcggaaga caacgtcatt ggcggattct
     6841 gtcagccgcg acggcgtcgg cgacgacgcc ctgtaccagt gccagcatcc tcgggtggtg
     6901 cctctggctg cacgtacgta cgcccggtca ggctgaggaa gacatcctca aggctcgccc
     6961 gcgtgctgga gagctcgccg agggtgatcc cgtgattcga ggcatgggtg agcagcccga
     7021 tgatcgtcgc ctgcgcgtcc cgcgtctgga gtttcaggcg gggaggcgtt tcactggtac
     7081 tgagtgatga ggacgtcacg ccgggaatgg acgcgagcgt cgactcatcg agcatctcgc
     7141 tcgcgccgga gatcgtggcg ctgaccgtcg cttcgagatc gaggtcgcga atgagccccg
     7201 ccggggtatc gcaggcaatg acgtggccgc cgtccatgat cgcgatgcgg tcgcagagga
     7261 cctcggcttc ttccatatag tgcgtggtca gcacgaccgt cgtgccggca tcgcggacgc
     7321 cgaggatcgt ttcccagaga ctgcgccgtg caagcgggtc gagcccggtc gtcggctcgt
     7381 cgaggaaggt cacttgcggc tggttgacga gcgcgagggc gatgctgagc cgctgctgct
     7441 ggccgccgga catctcctcg gcgcgcgcct ttcccttctc ctcgagcccg acgaggcgga
     7501 gcaacgtccc gacccgctcc ttcgggttgt cgctctcgta catgtggccg aagagctcga
     7561 tcagctcggc cgcggtcagg tacgggaaga gcgaggtcga ttgcagctgc acgccgatca
     7621 ccttccgcac atcgtccgat tgtttcacgg catcgaggcc ggcgacggtg atcgtgccgc
     7681 tgtcgtgagt gcgtaatcct tcgatcatct cgagcgtgga ggtcttgccg gcgccgttgg
     7741 gaccgaggat gccgaatatc tcgccggtgc ggacctcgag atcgatgttg tcgacggcgg
     7801 tgaatccctt gtaccgcttg gtcaggcccc tgatcgagat gatcggcggc gcggttgcgg
     7861 tcggggtggt tgatgaggat gagatcgagg tcatgaaacg taccccgggg acggaggtcg
     7921 aacggccgac ctgttgtcgg gcctacggca tgctagcacc ggccgcgcat tcctgccgtc
     7981 ggggattgtt ggaccgtttc gcatcggcga ggagtcaggt ggcgcgttcg gcgctggcga
     8041 gaaggtgctc ccagcgatcg ttcaccgggg agatgtcgaa ttcctcgacg agcatgcgga
     8101 cgaacatcgg aaccgtgacg ataccggtcg tcaggtgcgc tttgtggaac gaccacggcg
     8161 cacgccggtg atcccgctcg atgaatgagg atccgatgtg aaggtgcggt gcggtctgct
     8221 ttgcctgggg gtcgaacggc gtccagtggt aggcgacgac atcggatcgc atgtcggaag
     8281 acgcgccacg ggagagttga taggtgtatc tcgtgagttc gacccttggc gttccatgac
     8341 aggtgatctc gaggacacta ggactcgcca tcccgtaccg ggatgaagaa tgccaggtct
     8401 gcgactcggt agtcgtcgag gtcatattct ccggcgcggt agcgccgggt gaactcatct
     8461 gccccgacgc caaagaggga ttgagcgcgg tcttcgaaga tcgcacggta ctcctcgtcg
     8521 gtcatctcca tcaccggggg aagcgccgct tcgagttccg cgatttcctc ggaagtgaac
     8581 gtatagggtg ggctggattt cttgagcgcc atgaacttct cctttccagg atcaccccac
     8641 cattctatcc caacctccgc acgacacgag aaacccgagg atatcgatgc ggcaacgcga
     8701 ctgcgaccga agccgcatgc tgggacgggg gagttgctgg caacaacatc gtgatggccg
     8761 ctgcaacgag ggctcgggag gtcgcgaacg gaaacaacag tttcgcatcg gactgattgt
     8821 tctcgcagtc ctttcttgat ataagaagag gggaacgcgt ttcggaatcc ggtcgtcgtg
     8881 gctgcgtgcg cagcagatcg gtactccatc gcgccaatga ggttgatgcc atggatcccg
     8941 tttcgaccaa tgacctcccc cgcctcgatg tctccggtga gggcgacgac accgttcgct
     9001 ggaccgccgc cttcgtggcc tatggcgatc acggcgccga ccagacgacg acgctcgtct
     9061 tcgagatcga gccaggcggc cgtctcggct ggcataccga tcagaccgag gagacccagt
     9121 acatcatcgc cgggaccggc gaactccagc gcgatgacgc caccctcccc gttggccccg
     9181 gaagcgtctt cgtgctcccc acgaatgtcc gccacgacct cgtcaacacc gggaccgaga
     9241 cgctgcgcgc cgtcggcttc ttcggtgcgg cgatgttcac ccagcgcttc gacaatgtga
     9301 tgcttccccc gaagacgcac att
//
LOCUS       DASTMD010000911         5109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1517343, whole genome shotgun sequence.
ACCESSION   DASTMD010000911 DASTMD010000000
VERSION     DASTMD010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517343"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFQ54_13520"
     CDS             <1..453
                     /locus_tag="VFQ54_13520"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEU0166061.1"
                     /translation="VTSKRRAIGREVGRIVGFHRESSDDNWARRARSRMLASEMSSTS
                     APNREECMIRHTVVVRLHYPEGSEEEAGFFAALTALTQVPGVEKFELLRQVHPNTPYT
                     YGFSMEFADKPTADAYQIHPDHVAFVQKNWKNAVAEFMEIDYAPLELG"
     gene            609..1178
                     /locus_tag="VFQ54_13525"
     CDS             609..1178
                     /locus_tag="VFQ54_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166062.1"
                     /translation="MSRSQPQPFNVQFDFLPARHPRASRSETPRFAFANLPGGSGTAY
                     ERARQRLLLHALAVIPLAIAIAIVFVLVENRVIAYEGPQLGKGPITEFVIPAGTSTRI
                     TPITDSALDVPRQIAFGRTDNPTIVVRNEDTVPERAGPWIVQPGQSYRIRLGTAGIYE
                     FACTQNPSQSVDVIVNIDQVNGSGGTHKH"
     gene            1399..2046
                     /locus_tag="VFQ54_13530"
     CDS             1399..2046
                     /locus_tag="VFQ54_13530"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEU0166063.1"
                     /translation="MSGLENHMIDWVRSWFDSVGWFGVVIAMAIESACIPLPSEITMP
                     LAGWLLVDEHHLGWSGVFLAGFWGAVGNLIGSLIAYFVGAYAGRPIIERWGKWVLLTP
                     KDIDRADSFFVKWGEWAVFISRLLPVVRTFISIPAGIARMNLLRFSVLTFVGSFVWST
                     PLAAFGYKWGSDWEELRDKVRFLDYPIAALIVLAIAWFVWHRLKEMRAADEVAAS"
     gene            complement(2499..3194)
                     /locus_tag="VFQ54_13535"
     CDS             complement(2499..3194)
                     /locus_tag="VFQ54_13535"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU0166064.1"
                     /translation="MTDDDRLFASSNTIRDIKTGRDMGEQDRPVVLIVCDWNGTLVDD
                     AERACDATNVVLGQFALSNLSLGEFLEAFELPLERFFAGLGVADPDLATAGDVWNSEM
                     AAHPTRLARGAEAMLASALAYGLPVGVVSAAATEAVEADAEQLGIRSSLSFVTGRAAS
                     KRSVLQRLVEEADGRVLYLGDTEYDMIEAVAAGAIPIGVAGGYRPAAALSSAGATYIL
                     DDLGMLLTGMQGF"
     gene            complement(3354..4193)
                     /locus_tag="VFQ54_13540"
     CDS             complement(3354..4193)
                     /locus_tag="VFQ54_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0166065.1"
                     /translation="MRLGVLSDIHLAPAGSLPGMWHNPLHYERAPVLLDRAISFLRDR
                     DVDAVAVLGDLTNSGDRASIAQGLRLLGLLTVPVYVLPGNHDWGARGEVLRTTIEANQ
                     PSRIALPRGDRNGHFPFRLAGVTALDRRDEGITWHVRPGAIEADGDGPLLVMSHFPLL
                     DREQEIVAQGLKFAGDFAWSSSTRDPLLTRSAPTVLLHGHLHIRYATTEGTILQLGFA
                     SLIEAPHEVSLVEIAERDGELTVSVEHHSVETDDVPVVPLLSASRSIWSFQDHRWTND
                     VGD"
     gene            complement(4226..>5109)
                     /locus_tag="VFQ54_13545"
     CDS             complement(4226..>5109)
                     /locus_tag="VFQ54_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0166066.1"
                     /translation="DALRRFARNKVAVAGALIVALLLLIAIVGPMLSPYDYARQDYLA
                     VNLPPSSHHWLGTDALGRDYMTRVMMGGRTAFLLSIWVVAITTILGALLGAASGFFGG
                     WVDAIIMRIGDTFMAFPQLLLALFCAGAIRPPVAKWLANYRFFQSNSYLVDYAIVFGA
                     LSVVGWSGMARLMRAQIMMLRRQEYVTASESVGASNWRILSRHLLPNAMSTVVVSATA
                     SIANVMVLESSLSFLGIGVRPPAASWGSMIGENLTTWQHSPHLLAAPAIVLAIAALAF
                     NFIGDGLNDALDPRRQT"
BASE COUNT          909 a         1655 c         1676 g          869 t
ORIGIN      
        1 gtgacctcga agcgacgcgc gatcggtcgc gaagtaggca ggatcgttgg atttcacagg
       61 gaatcctcag acgacaactg ggcgaggcga gcccggtcgc gtatgctggc gagcgaaatg
      121 tcgtcgacca gcgcgccgaa ccgggaggaa tgcatgatcc gccacaccgt tgttgttcgt
      181 ctgcattacc ccgaaggctc agaggaagaa gccggcttct ttgcggcact cacggcgctc
      241 acccaggtgc cgggggtcga gaagttcgag ctgttgcgac aggtccaccc gaataccccc
      301 tacacgtatg gcttctcgat ggagttcgcc gacaagccga cggccgatgc ctatcagatt
      361 caccccgacc acgtcgcgtt cgtccagaag aactggaaga acgccgtcgc cgagttcatg
      421 gagatcgact acgccccgct cgaactcgga taacccctcc tgctatcaca atgacgtgat
      481 tcgatggatg ccccggtcaa tggcgaccgg ggcatccatc gtttgcgttg cgcgtggtgt
      541 acgtccacgg atcgacccga cgtgctaccc tcttcctgat gcgtctcctc agcgaaggac
      601 tccgcgccat gtcgcgttcg caaccacaac cattcaacgt ccagttcgac ttcctcccag
      661 cgcgacaccc ccgggcgtcc cggagcgaaa cgccgcggtt tgcattcgcg aacctgcctg
      721 gcgggagtgg aacagcctat gagcgggccc ggcagcgcct cctcctccat gcgctcgcgg
      781 tgattccgct ggcgatcgcg atcgcgatcg tcttcgtgct ggtcgagaac cgcgtcatcg
      841 catacgaggg gccgcaactc gggaaggggc cgatcaccga gttcgtgata ccggccggca
      901 cctcgacgcg gatcacgccg atcacggatt ccgcgctcga cgttccccgc cagatcgcct
      961 tcggtcgcac cgacaacccg acgatcgtcg tccgcaacga ggacaccgtg ccggagcggg
     1021 ccggtccgtg gatcgtgcag ccgggtcaga gttacaggat ccgtctcggc actgccggca
     1081 tctacgagtt cgcctgcacc cagaatccat cccagagcgt cgatgtgatc gtcaacatcg
     1141 atcaggtcaa cggctccggc ggcacgcaca agcactgatg gcccgtggcg gttcatcggt
     1201 agttcatccg gacatctgac aatgtcacga gcccgtcccg aatcgcggac gcgtggggcc
     1261 gggcagtgta tcttggcgtt ggcatggtgt gactgcagga tgcgcggcgg ctgtgaccgg
     1321 ggtgggagac gaaaccgatc tggcggtggc ggcacgtacc gccgttgact ttcgcatttg
     1381 tgatctggag tctgcttcat gtcagggttg gaaaatcaca tgatcgactg ggtgcgatcg
     1441 tggttcgatt ccgtgggctg gttcggcgtg gtgatcgcga tggcgatcga gagcgcctgt
     1501 atcccgctcc cgagtgagat cacgatgccg ctcgccggct ggttgctcgt cgacgagcat
     1561 cacctcggct ggtcgggcgt gttcctcgcc ggattctggg gagcggtcgg caacctgatc
     1621 ggctccctga tcgcctattt cgtcggcgcc tatgctggtc gcccgatcat cgagcgatgg
     1681 gggaagtggg ttctcctcac accgaaggat atcgaccgcg ccgacagctt cttcgtgaag
     1741 tggggtgagt gggcggtctt catctcccgc ttgctgccgg tggtccgcac cttcatctcg
     1801 attccggccg gcatcgcgcg gatgaacctc ctccgcttca gcgtcctgac cttcgtcggc
     1861 tccttcgtgt ggtcgacccc gctggcggcc ttcggctaca agtggggatc ggactgggaa
     1921 gagctgcgcg acaaggtgcg cttcctcgac tatccgatcg ctgcgctgat cgtgctcgcg
     1981 atcgcctggt tcgtctggca tcgcctcaag gagatgcggg cggcggacga ggtcgcggcg
     2041 tcgtagatca acccgacaga cttcgtgacg gacaggcaaa gacccgccag gcatatcgcc
     2101 ctgcgggtct ttgcctgtcc tcccctcgcc tgagtccccc agacacgtct tcccgagcct
     2161 gtcgagggac cagccggcat ccacctgccg tgccgacaac gccccccaag gcacgtcctc
     2221 ccgagcttgt cgagggacca gctggcaccc acgtgctccc ggcccggcgg cgaccacgtg
     2281 ggtcgccagg acggaaatgg ggtcagttgg cgcccacgtg ttcccggcac ggcaaggcgt
     2341 tccccggatg cggatcggca tcgccgatcc ggcggcgacc acatgggtcg ccgggacggg
     2401 aatgggagac gcggggcccc aacatcaatg cagtgcgcct tgctgcgtcg catcgatccg
     2461 aaacggcgac aagcgccgtg gggacgggac ggggagtgct agaatccctg cattcccgtc
     2521 aggagcatcc ccaggtcgtc caggatatag gtcgcaccgg cgctggagag tgcggccgcg
     2581 ggccggtaac cgcctgccac cccgatcggg atcgcccccg cggcaaccgc ctcgatcatg
     2641 tcgtattccg tgtcgcccag atagagcact cgtccatcgg cctcctccac caggcgctgc
     2701 agcaccgagc gcttgctcgc ggcacgcccc gtcacaaagc tgagactgct gcggattccg
     2761 agctgctcgg cgtcagcctc gacggcttcg gttgccgcgg cgctcaccac tccgaccggc
     2821 agaccgtacg cgagcgcgct ggcgagcatg gcctccgcgc cgcgggcgag ccgggtcgga
     2881 tgggccgcca tctcgctgtt ccagacgtct ccggctgtgg cgagatccgg atcggcgact
     2941 ccgagtccag cgaagaaccg ctcgagcggt agctcgaagg cttcgagaaa ctcgccaagg
     3001 ctgagattcg agagggcaaa ctggccaaga accacgttgg tggcatcgca agctcgttcc
     3061 gcgtcgtcaa ccaaggttcc attccagtcg caaacgataa gtacgacggg tcgatcttgt
     3121 tctcccatgt ccctgccagt tttgatatct cgaatcgtgt tcgaagaggc gaatagtcga
     3181 tcgtcgtcgg tcatgggcca acacgccgcc ggacgcggcg cgcccgcgat acagcgaaat
     3241 ccgtttccgt cacgacgggt gtggatgctg gaggccggga agcatcgcgc gggccaccgg
     3301 aaacggtgac ggacgccacg gtcatcacgg aggcaagcgt acccgatttc gcttcagtcg
     3361 ccaacgtcgt tcgtccagcg atgatcctga aacgaccaga tcgagcgcga cgcggagagc
     3421 agggggacga cggggacgtc gtccgtctcg acactgtggt gctcgacgga gacagtcagc
     3481 tccccatccc gctcggcgat ctcgaccagg ctcacctcgt gcggcgcctc gatcagcgac
     3541 gcgaagccga gctgcaggat cgttccctcg gtcgttgcgt agcgaatgtg gagatgaccg
     3601 tgcaggagaa ccgtcggggc gctacgcgtc agcaggggat cgcgggtact gctcgaccag
     3661 gcgaaatcac cggcgaactt caggccctgc gcgacgatct cctgctcccg gtcgagcagg
     3721 ggaaagtggc tcatgaccag cagcggccca tcgccatcgg cttcgatcgc ccccggacgg
     3781 acgtgccagg tgatgccctc gtcacgccgg tcgagtgccg tgacgccggc caggcggaac
     3841 gggaagtgac cgttgcgatc gccgcgtgga agcgcgatcc ggctcggttg gttcgcctcg
     3901 atggtggtgc gcaggacctc gcctcgcgcg ccccagtcgt gattgccggg caggacgtag
     3961 accggcacgg tgaggagccc gagcagacgc agaccctggg cgatcgacgc gcgatcgcca
     4021 gagttcgtca ggtcgccaag cacggcgacc gcatcgacat cgcggtcccg gagaaacgag
     4081 atggcgcgat cgagcagaac cggggctctt tcataatgca gcgggttgtg ccacatccct
     4141 gggagcgagc ccgcgggtgc gaggtgaata tccgaaagta cgccgagtcg catcaattgt
     4201 ccctttgtcc ctatgccacg ttcgatcagg tttgtcgtcg tgggtcgagg gcgtcattca
     4261 agccatcgcc gatgaagttg aaggcgaggg cggcgatcgc gagcacgatg gccggcgccg
     4321 ccagcagatg cggcgagtgc tgccaggtcg tgaggttctc gccgatcatg ctgccccaac
     4381 tggcggccgg cgggcgcacg ccgatcccta agaagctcag cgacgactcc agcaccatca
     4441 cgttcgcaat actggccgtc gccgagacga cgaccgtcga catcgcgttc ggcaacaggt
     4501 gccggctcag gatccgccag ttcgaggcac cgaccgactc gctggcggtg acgtattcct
     4561 ggcggcgcag catcatgatc tgcgcgcgca tcaggcgcgc catgcccgac cagccgacga
     4621 cggagagcgc cccgaagacg atcgcgtagt cgacgagata ggagttggac tggaagaagc
     4681 ggtagttggc cagccacttc gccaccggtg gccgaatcgc gccggcgcag aagagcgcca
     4741 gcagcagctg cgggaaggcc atgaaggtgt cgccgatccg catgatgatc gcatcgaccc
     4801 agccgccgaa gaagccactc gccgcgccga gcagcgcgcc gaggatcgtg gtgatcgcca
     4861 caacccagat cgagaggagg aaggcggtgc ggccgcccat catcacccgc gtcatgtagt
     4921 cgcgcccgag cgcgtcggtc ccgagccagt ggtgactgct cggcgggaga ttgacggcga
     4981 ggtaatcctg ccgcgcgtag tcgtagggcg agagcatcgg cccgacaatc gcgatcagga
     5041 gcaggagggc gacgatgagc gcgccggcga cggcgacctt gttccgggcg aagcgccgca
     5101 gggcgtcgc
//
LOCUS       DASTMD010000912         1692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_354333, whole genome shotgun sequence.
ACCESSION   DASTMD010000912 DASTMD010000000
VERSION     DASTMD010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354333"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VFQ54_13550"
     CDS             complement(<1..633)
                     /locus_tag="VFQ54_13550"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU0166067.1"
                     /translation="MKITPSGLAGASARHPWRTIGLWIVLLAVITVLSGVIGGNTNSQ
                     NLGFTNSPDSQKADDLIKDHFKADENASEVIVFHSDSLTVNDPAFKGVVTKTIANLDQ
                     SSWKDEIASVTNYYEAGAAGTDLISGDSHSLIVPISFTQKGDEYTDDGENYVKLAQGA
                     ATSDVNVYTVGDISGDYTFGKIAGEDTNKDTTIGLPVAGIVLIIVFGALIA"
     gene            complement(696..1310)
                     /locus_tag="VFQ54_13555"
     CDS             complement(696..1310)
                     /locus_tag="VFQ54_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166068.1"
                     /translation="MSPEMSSTSSTRPDERASTNSDQTKSPMEEVANRLDAARTIELE
                     IARHQEELRVFEQQFAGLRALRDELRGMVSDARTVASVAGASELDRAMGLVIAALNRT
                     AVTTSGSSENLAAVERLAGEVLVAAMMNVANHSAATESNFSIKREESWLFISVFDNGN
                     GNADPGRGASLRLLRERIDAAGGHFSLSSPPGRGTLLTVGLPCT"
BASE COUNT          291 a          499 c          554 g          348 t
ORIGIN      
        1 ggcgatcagg gcgccgaaga cgatgatcag gacgataccg gcgaccggga ggccgatggt
       61 cgtgtccttg ttcgtgtctt ccccggcgat cttgccgaag gtgtagtcgc cactgatgtc
      121 gccgaccgtg tagacgttga cgtcgctggt cgccgcgccc tgtgcgagct tgacgtagtt
      181 ctcgccatcg tccgtatatt cgtcgccctt ctgcgtgaag ctgatgggaa cgatcaggga
      241 gtgggaatcg ccggagatca ggtcggttcc cgcggcaccg gcttcgtagt aattcgtgac
      301 ggacgcgatc tcgtctttcc agctgctctg gtcgagattg gcaatggtct tcgtcacgac
      361 gcctttgaat gccggatcgt tgaccgttaa tgaatccgag tggaagacga tgacttccga
      421 agcattctcg tcggccttga agtgatcctt gatcagatcg tcggccttct gcgagtcggg
      481 actattcgtg aatccgaggt tctggctgtt ggtgttgcca ccgatgacgc cggagagaac
      541 cgtgatgacg gcaaggagaa cgatccagag tccgatcgtc cgccaggggt gacgcgcact
      601 cgcgcctgcg agacctgaag gggtaatttt catgggggag actcctctga gcgttggaat
      661 ccagatggaa gggggtcagt cgagaactcg gtttctcagg tacagggcag gccaacggtc
      721 aggagcgtgc cgcgtcctgg cgggctgctg agcgagaagt gaccgccggc cgcgtcgatc
      781 cgctcccgaa gcaggcgcag gctggcgccg cgtccgggat ctgcattgcc gttcccgttg
      841 tcgaagacgg aaatgaagag ccagctctct tcgcgcttga tgctgaagtt ggattccgtt
      901 gcggcactgt ggtttgcgac gttcatcatc gcggcgacga ggacctcacc cgccagtcgt
      961 tcgaccgcgg caaggttctc cgaactaccc gatgtcgtca cggccgtccg gttgagcgcc
     1021 gcgatgacga ggcccatcgc gcgatcgagt tcgctcgcgc ccgcgacact ggcaacggtt
     1081 cgcgcgtcgc tgaccattcc cctcagctcg tcccgcagcg cgcggagtcc cgcgaattgc
     1141 tgctcgaaga cacgaagttc ctcctgatgc cgcgcgatct ccagctcgat cgtgcgcgcg
     1201 gcgtcgaggc gattcgcaac ctcctccatc ggggatttgg tctgatcgga attggtcgag
     1261 gcccgctcat ctggcctggt tgacgacgtg gatgacattt ccggtgacat gatgggctcc
     1321 cctcggtact tcggtggcag gttggtgacg gccaggaacg cctctcgtgc gttcgtttga
     1381 cctgtctcga ttctgccgtc tggcgccgat cgttcgaagc cagcgttccc gaaaaaccgt
     1441 gcgacggtta tcccccacgg gatggggtgg aaacgcgcct tctgactggg ggtcgcgaag
     1501 agccccgtca ccagattggt gacggggcgt cgtccgggcg gacacgtagg gagcttcaga
     1561 tccggcagga tgacgccgaa gtcaatggat tccggcaagg cccgaatcgg gtccagctgg
     1621 cctggatcgc catcaggagg gcatcgccac ccgatgccgg tctgccacat caggccaggc
     1681 ccgaacgacg ac
//
LOCUS       DASTMD010000913         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1068468, whole genome shotgun sequence.
ACCESSION   DASTMD010000913 DASTMD010000000
VERSION     DASTMD010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068468"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..271
                     /locus_tag="VFQ54_13560"
     CDS             53..271
                     /locus_tag="VFQ54_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166069.1"
                     /translation="MNRPQPNPLPPNELYVVGERKDNPIELLLQGTDGRYYVYQPPDE
                     DPKPVDVAEDSAWQIEDTLPTDIKVEPD"
     gene            381..950
                     /locus_tag="VFQ54_13565"
     CDS             381..950
                     /locus_tag="VFQ54_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166070.1"
                     /translation="MKTLFVSLVAVLISLGAFASYVAADPATPDLPAGATPPAMAAEG
                     YPVAIHAGSCTGAVGESAYPIANTVLVGQQDTTQSPSGGAVDQPVVTSSATITANLDD
                     LASGSDVITVHASPADYDTIIACGRISGPVTNGQLVVAITSLHGSGMTGIAVLEDSRS
                     GVAGIGNGQVLVTVYLIDPTLESVATPAS"
     gene            complement(1137..>2027)
                     /locus_tag="VFQ54_13570"
     CDS             complement(1137..>2027)
                     /locus_tag="VFQ54_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU0166071.1"
                     /translation="DLLTLMKSDVESPGLLIDIKRIAELDDLIELTSDGLRLGALATI
                     SEIQHDPLVRSLHPALAEAAGVAATPQLRNMAMIGGNLLQRPRCWYFRDSEISCWLKG
                     GDTCPAAEAEGENQFHAILGESPCHAVHPSDLATVLVALDARIVVRGKREERTLPIEE
                     FFSLPTEHRRIEHTLAKDELIVAIVIPDVPSATRSTYLKAMDRKVWAFALVGAAVQVT
                     IADGIITRARVVLGGVAPIPWRVPAAESALIGSAADPDLLERAAAIAVAGARPLSKNA
                     YKVQLAKTLMERALATAMAV"
BASE COUNT          378 a          667 c          626 g          356 t
ORIGIN      
        1 gctctgggaa aggtatttcg ccgcccaccg ctcgtgacaa gcaggaacag tgatgaaccg
       61 cccgcaacca aaccctctcc cacccaatga gctctacgtc gtcggtgaac ggaaggacaa
      121 ccccatcgag ctcctccttc agggcaccga tggcaggtat tacgtctacc agccgcccga
      181 tgaagatccc aaacctgtcg atgttgcgga ggattcagcc tggcagatcg aagacacgct
      241 ccccacggac atcaaggtgg aacccgactg actgctcatg tggagaacgt gcctgctctc
      301 gaggcgaagt ggccccgatc ctgcaaacgg cctgcctcag gcggagaaaa acgacgttcc
      361 gcagatgcag gagggacccc gtgaagacac tgtttgtgag cctcgtcgct gttctgatct
      421 cgctcggcgc attcgcatcg tacgtggcgg ctgatcccgc aacgccagac ctgccggcgg
      481 gcgcgacgcc tccggcgatg gcggcggaag gctaccccgt cgcgattcac gcgggatcat
      541 gtacgggagc cgtcggtgaa tcggcgtatc cgatcgccaa tacggttctg gtcggccagc
      601 aggacacgac gcagagtcct tcgggtggcg ccgtcgatca gccggtggtg acctcgtccg
      661 ccaccatcac ggcgaacctg gacgacctcg cgagcggctc cgatgtcatc accgtccacg
      721 cgtcgccggc cgactacgac acgatcatcg cctgtggtcg gatctcgggg cctgtgacga
      781 acggccagct cgtcgttgcg atcacctcgc tccatggatc ggggatgacg ggtatcgcgg
      841 tgctggagga cagtcgttcc ggcgtcgcag gaatcggcaa cgggcaggtg ctggtcacgg
      901 tctatctgat cgatcccaca ctcgagtcgg tcgcgacacc ggcatcctaa cgcctgattc
      961 gatcaacgcc agagcaacat ccccggtcca gggaccgggg gtgttgctga cgatgattcc
     1021 gctccccgac tccgagatca taccggcctc cagggtctcg ataccgcgca ggtaggcccg
     1081 ttccgggcgc gcgggaacga tgccggttgc gaggacggga tggccgcgat gcaggatcag
     1141 actgccattg ctgtcgccag cgcccgttcc atcagcgtct tcgcgagctg caccttgtag
     1201 gcattcttcg acaacggtcg tgccccggcc acggcgatag ccgccgcccg ctcaagaagg
     1261 tcgggatcag cggcgctgcc gatgagcgcg gactcggcgg caggcactcg ccagggaatc
     1321 ggagccaccc ctcccagcac cactcgtgcc cgcgtgatga tcccatcggc gatcgtcacc
     1381 tgtaccgccg caccgacgag tgcaaatgcc cacaccttgc gatccatcgc cttgaggtag
     1441 gtgctgcgcg tcgccgaagg aacatcggga atcacgatcg cgacgatcaa ctcatccttt
     1501 gccaacgtgt gctcgatccg gcgatgctcc gtcggaaggg agaagaactc ctcgatcggg
     1561 agcgtgcgct cttctcgctt gccacgcacg acgatgcgcg catcgagggc gacgagcacc
     1621 gtcgcgagat cggagggatg caccgcatgg cagggactct ccccgaggat cgcgtgaaac
     1681 tggttctcgc cttcggcctc tgccgccggg caggtatcgc ctcccttcaa ccagcaggaa
     1741 atctcgctat cccggaagta ccagcatcgg ggtctctgca acaggttgcc gccgatcatc
     1801 gccatgttac gcaactgcgg cgtggcggcg acacctgccg cctccgccaa ggctggatga
     1861 aggctccgta cgagcgggtc gtgctggatt tcggagatcg tcgccagtgc gccgaggcgc
     1921 aggccatccg aagtcagttc gatcaggtca tccagttcgg cgatccgctt gatgtcgatc
     1981 agcagtcccg gggattcgac gtcgcttttc atcagcgtga gcaggtc
//
LOCUS       DASTMD010000914         1793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2051025, whole genome shotgun sequence.
ACCESSION   DASTMD010000914 DASTMD010000000
VERSION     DASTMD010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2051025"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="VFQ54_13575"
     CDS             <1..1424
                     /locus_tag="VFQ54_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEU0166072.1"
                     /translation="DPTGAILGLARRTYETAHPRPGWSEQNPADWWRATTEVVRELAS
                     ALPDTRLAAISLTGQMHGTVLLDADGAAIGPAIIWSDRRSDAQRDEIARAVGPELQAR
                     IGGPLGVGYMAATIRWVRDCQPELAAKIAAVLLPKDALGFRLTGIRATEPSDAISTGL
                     LDAESWTWNPVLLEAAGVDAPWLADVLPSGSIAGRLLPGLATELGLEPGIPVVLAGGD
                     APCAAFGAGVTGSDEAMVVLSSGAQVILPTPGFAPDADGRWHTFPSAVPPTGSGQPRN
                     RVCATSNAGIALEWLAAITGTGVPTLLDRASSVAPGAERALFIPYLTGQRTPILDSLA
                     RGSFFGLTDRHTSADLARAVAEGIIFACRHAYATIVAGGNSPSTIRLGGGPSQHPLIR
                     QLVADIFNLPVQPLESPDLTVIGAARSGALALGWADPEPRFDTSTVVTPEPGRVAFYD
                     RLFPLHIDAAEAGREISHRLQAL"
     gene            1455..>1793
                     /locus_tag="VFQ54_13580"
     CDS             1455..>1793
                     /locus_tag="VFQ54_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166073.1"
                     /translation="MTPRLAPYPLKLTTPIRRYNFGERLIPNILQKGGLPDGVVAETW
                     EFSDEKSAPATIVNGHLAGRTLHDVTLSEPDWLVGEGWSGPAYPLLGKFLDASHALPV
                     HLHADDEIARA"
BASE COUNT          284 a          668 c          548 g          293 t
ORIGIN      
        1 tcgatcccac gggagccatt ctcggactcg ccaggcgcac ctacgaaacg gcccacccgc
       61 ggccaggttg gagcgaacag aatcccgccg actggtggcg tgcaaccacg gaagtcgtcc
      121 gcgaactcgc atcggcgctt ccggataccc ggctcgccgc gatcagtctc accggccaga
      181 tgcacggcac ggtccttctc gatgctgacg gcgccgcgat cggtccggcg atcatctggt
      241 ccgaccggcg ttccgatgcg cagcgcgacg agatcgctcg tgccgtgggt cccgagctcc
      301 aagcccggat cggcggcccg ctcggtgtgg gctacatggc tgccacgatt cgctgggtcc
      361 gcgattgcca gccggaactg gccgcgaaga tcgccgctgt cctgctcccg aaggatgcgc
      421 tgggttttcg cctcaccggc atccgcgcga ccgaaccgag cgacgcgatc agcaccggtc
      481 tcctcgacgc ggaatcctgg acgtggaatc ccgtcctgct cgaggccgcg ggagtcgacg
      541 cgccctggct ggccgatgtc ctcccctcgg gcagcatcgc cggacgcctg cttcccggac
      601 tcgcgacgga actcggactc gaacccggca tcccggtggt cctcgccggt ggcgacgccc
      661 cgtgcgcggc cttcggcgcc ggggtcaccg ggagcgacga ggcgatggtc gtcctcagct
      721 ctggcgccca ggtgatcctg ccgacgcctg gtttcgcgcc ggacgcagac ggacgctggc
      781 acacgttccc cagtgccgtc ccaccaacgg gatcgggtca gcctcgcaac cgcgtctgcg
      841 ccacgtcgaa tgcggggatc gccctcgaat ggttggcagc gataaccgga accggagtgc
      901 caacgctcct cgatcgcgcc agttccgtgg caccgggagc ggagcgcgcg ctcttcatcc
      961 cttacctgac gggacaacgc acaccgattc ttgattcact cgcccggggc agtttcttcg
     1021 gactgaccga tcgccataca tccgccgatc tcgcgcgtgc cgtggccgaa gggatcatct
     1081 tcgcctgtcg ccacgcctac gccacgatcg tcgccggcgg aaactcccct tcgaccattc
     1141 gcctcggcgg aggcccgagc cagcatccgt tgatccggca actcgtggcc gatatcttca
     1201 atcttcccgt ccaaccactc gaatcccccg acctgaccgt cattggcgct gcccgcagcg
     1261 gtgcgctggc actcggctgg gcggatcccg agccgcggtt cgacacatcg accgtcgtaa
     1321 caccggaacc agggcgcgtt gccttctacg atcgcctctt cccgctccac atcgacgccg
     1381 ccgaagccgg tcgcgagatt tcgcatcgtc tccaggctct ctagtcgccc gcctcgaaag
     1441 gaccacccgc cgcaatgacg ccgcgcctcg caccctatcc cctgaaactg acgacgccga
     1501 ttcgccgcta taacttcggc gagcgcctga tcccgaacat cctccagaag ggtgggctgc
     1561 ccgatggcgt ggtggccgag acatgggagt tcagcgacga gaagtcagcc ccggcgacca
     1621 tcgtgaacgg ccacctcgcc ggacggacgc tgcacgacgt gaccctgagc gaaccggact
     1681 ggctcgtcgg tgaaggctgg tcgggtcctg cctacccgct gctcggcaag ttcctcgatg
     1741 ccagccacgc gctccccgtc catctccacg ccgatgacga gatcgcgcgg gcg
//
LOCUS       DASTMD010000915         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1783915, whole genome shotgun sequence.
ACCESSION   DASTMD010000915 DASTMD010000000
VERSION     DASTMD010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783915"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFQ54_13585"
     CDS             <1..690
                     /locus_tag="VFQ54_13585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0166074.1"
                     /translation="FRSSAPLVPLRLFQSRGLTVSSGAIALNGAAFLSMFFLTAVYLQ
                     NVRGDSALKTGLEFLPMGVAAILAALAASQFVTKIGTRPIQLAGSVIGVIGLILLMQA
                     GPGGSYVVHLLPGLVLFGIGIISVGVPAQITAVADVTGHDAGAASGVVTAFNQVGGAI
                     GLAIVTTLANSRTTDVLAGGASQIDALVQGFHRGLLICAIYAAVTIAITILSPRVRPT
                     AEQLLEATAAA"
     gene            complement(733..1173)
                     /locus_tag="VFQ54_13590"
     CDS             complement(733..1173)
                     /locus_tag="VFQ54_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166075.1"
                     /translation="MDAIFNERTLLLVAHIGAVILLIGPVIVATSLFPRYASPENAAV
                     AGALHRITRVYGATSLMVPGIGVILAQRFGLLGAGWIVAALVLFTVAFGLLIGFIVPR
                     QDRLLQTLLDRTSPPADLASLRMASGMLNLIWLVILVLMVAKPT"
     gene            1284..1922
                     /locus_tag="VFQ54_13595"
     CDS             1284..1922
                     /locus_tag="VFQ54_13595"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0166076.1"
                     /translation="MKHEDPRVQRSRAAILDAAVSLFVEGGIRAVTVDEVALRSGVAK
                     TTIYRHWPNREALLMDVFRRFDHRFSAPQDGQTPEAYLRVVMEELVATMAEERWRQAL
                     PALLDVARRVDEFAELHASMPPGGGSISRAIVRLIEAGVLPPVDPGEVLAQLVGPLTM
                     AAMFAPAALNDAFANGIVDRLIAGYAATMGGIAPVRESPVITDPTDGRGTAA"
     gene            complement(1927..2433)
                     /locus_tag="VFQ54_13600"
     CDS             complement(1927..2433)
                     /locus_tag="VFQ54_13600"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0166077.1"
                     /translation="MDLLDRLLGHDQWTTSRLLEMSGSLTDAQLDQPFDIGHRTLRAT
                     FDHMISAVDFWTTAMLGRPEPMHEYENGTPQSLEALRAWHERAYAAFSSLARQLQAEQ
                     RLDEIYRDYYDYPQSIGATIIQVALHDQQHRSEVLHILQRLGLTDLPDGDAQEWEHLT
                     GRIPSPDA"
BASE COUNT          461 a          873 c          974 g          505 t
ORIGIN      
        1 ttccggtcat cggctccgct cgtgccgctt cgtctcttcc agtcgcgggg gttgaccgtc
       61 tcgagtgggg cgatcgccct caatggcgcg gctttcctct cgatgttctt cctgaccgcc
      121 gtctacctcc agaacgtccg cggtgacagt gcgttgaaga cgggactcga gttcctgccg
      181 atgggcgtcg cggcgatcct ggcggcgttg gccgcctcgc aattcgtgac aaagatcggg
      241 acgagaccga ttcaactggc cggaagcgtg atcggggtga tcgggctcat cctgctgatg
      301 caggctggcc cgggtggcag ctacgtcgtc catctcctgc cgggtctcgt tctcttcggg
      361 atcggcatca tcagcgtcgg cgtgcccgca cagatcacgg ccgtcgccga cgtgaccggt
      421 cacgatgcgg gcgcggcatc cggtgtggtg accgccttca accaggtcgg cggcgcgatc
      481 gggctcgcca tcgtcacgac gctcgccaac tcgcggacga cggacgtcct cgctggcggt
      541 gcgtcgcaga tcgacgcgct ggtgcaggga ttccatcggg gactgctgat ctgcgcgatc
      601 tacgcggcgg tcaccatcgc gatcacgatt ctgtcgccac gggtgcgccc gacggcggag
      661 caattgcttg aagcaaccgc cgccgcgtag gctcggcgga gcctcgcttc cactggagtg
      721 gcgcgtgagg tctcaggtcg gcttcgcgac catcaggacg agaatgacga gccagatcag
      781 gttcagcatt cccgacgcca tgcgcaagga agcgaggtcg gcgggtggcg acgttctgtc
      841 cagcagggtc tggagcaacc gatcctgcct gggcacgatg aatccgatga ggagcccgaa
      901 cgcgacggtg aagagtacga gggcggcgac gatccagccc gcgccgagca ggccgaaccg
      961 ttgagcgagg atcacgccga ttcccggcac catcaggctc gtcgcgccgt agacgcgggt
     1021 gatgcgatgc agcgcgccgg cgaccgccgc attctcgggg ctcgcgtacc gtgggaagag
     1081 gctggtcgcc acgatcacgg gaccaatcag aaggatcacg gcgccgatgt gggcgacgag
     1141 caggagcgtg cgctcgttga agatggcgtc catcaggtct cccgggatca caggagcgat
     1201 ccgtcgcgaa ggcgtgtaag atacgatacc gtagcgtagc gtaaacgctc catggcggga
     1261 tgacaaggtg atatagttcg gcaatgaagc acgaagatcc ccgcgttcaa cgatcccggg
     1321 cggccattct cgatgctgcc gtcagcctct tcgtggaggg ggggattcgt gccgtcacgg
     1381 tcgatgaggt ggcgctacgg tcgggcgtgg caaagaccac gatctaccgg cactggccaa
     1441 accgtgaggc gttgctgatg gacgtcttcc gccggttcga ccaccggttc tcggcgccac
     1501 aggatggcca gactcccgag gcgtatctcc gggtggtgat ggaggaactc gtggcgacca
     1561 tggcggagga gcgctggcgg caggcgctgc cggcgctgct ggatgtggct cgccgagtcg
     1621 acgagttcgc cgaactccat gcgtccatgc caccaggcgg tggatcgatt tccagggcca
     1681 tcgtgcgcct gatcgaggcg ggtgtcctgc cgccggtcga tcccggggaa gtcctggcgc
     1741 aactcgtcgg ccccctgacg atggccgcga tgtttgcgcc ggccgcattg aacgacgcct
     1801 tcgccaacgg catcgtggat cgtctgatcg ccggctacgc ggcgacgatg ggcggaattg
     1861 cgccggttcg cgaaagcccc gtcatcaccg acccgacgga cggcagaggg acggccgcgt
     1921 aactgctcag gcgtccggcg acgggatgcg gcccgtcagg tgttcccatt cctgggcgtc
     1981 gccatcgggt agatcggtca ggccaaggcg ctgaaggata tgcagcacct cgctgcgatg
     2041 ctgctggtcg tgcagcgcga cctgaatgat cgtggcgccg atcgattgtg ggtagtcgta
     2101 gtaatcgcga tagatctcat ccagtcgctg ttcggcttgg agctgtcgcg cgagtgacga
     2161 gaatgcggcg tatgcgcgtt catgccatgc acgcaacgct tcgagcgatt ggggcgtgcc
     2221 attctcgtac tcatgcatcg gttctggtcg cccgagcatc gccgtcgtcc agaagtcgac
     2281 ggcggagatc atgtggtcga acgtcgcccg caaggtgcgg tgtccgatgt cgaaaggctg
     2341 atcgagctga gcgtcggtca ggctcccgct catctccagg agccgggatg tcgtccactg
     2401 gtcgtgtccc aagaggcgat cgagcagatc cactggtttc cctccatgcg gatgtcgtcc
     2461 aacgtgtgac gatcatagcg tagcggcaaa tgcggtcgtt tcctgtcctt tcgtaggggc
     2521 gagtctcgtg ctcgccctcc gccccgttca acgagctcag ggcgggctcc ggcggacagc
     2581 ggcggaatct cccacgcgcg acgcgctccc ggaagcaggt cggctgcgcc gcattgaccc
     2641 gaggcgacca cgcaggtcgc cgggacggga cccgagcgcg tgccggcggc aacgcggtcg
     2701 cgcgtccgag gggacgtcac cgtcctctct cacagcgacg cgcggaacca tcgacaggtc
     2761 tggtggccct ggggttggta gggcggggtt tcataccccg ccgctaagcg agt
//
LOCUS       DASTMD010000916         2955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_442891, whole genome shotgun sequence.
ACCESSION   DASTMD010000916 DASTMD010000000
VERSION     DASTMD010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442891"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /locus_tag="VFQ54_13605"
     CDS             <1..1673
                     /locus_tag="VFQ54_13605"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU0166078.1"
                     /translation="VAWSYDLLPPAEQSAFRELSVFTGGFTIETAAAVLDAESSSPGE
                     VPVGVLDLVASLLDKSLLRKREGSGRTEPRLSMLVTVGEYGHEQLRAMGEYESAHDRH
                     ARWALAFAETSARHLAGPDASRWLHELDDEQGNIRLAFRWLDASGDRSRFVRLALALE
                     DYWLTRARYSEALQWLGRAMELSADERDVPDARLRCTLAAAWIALRGGDGMRATVLAD
                     AGLALARENSDVRLVVRSLNVAAGVARRADDHQRAFDLIEESRRRSEAAGDRRAVADA
                     LRESAYALMNLGRMEEAIEACRASIAYFEEVGNTQSAAMALDNMSLVYYLRGDYAEAH
                     RCSREAIEVFRRLGYPRGVGIALCHLGLSATHLGDVEAAWNFHLEELAHRASVGDPRG
                     YAVWLEAVALLLAVCGDGTGALTILGAADAARERAQAPLARTERGDRDHSLRLVRRQL
                     TDDAIDAALRQGQSLAIPAAIERACALVPVAIAGYRKPEPPSARSAFGLSAREEEILQ
                     LVAQRLSDREIAEALFISRHTVARHVANILRKLDVSSRYDAARIANEH"
     gene            2537..>2955
                     /locus_tag="VFQ54_13610"
     CDS             2537..>2955
                     /locus_tag="VFQ54_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166079.1"
                     /translation="MATANLDVVMRRVLRSGAPVIRLAVIAAMSCALILASSVMAQGS
                     VTPTPVATLTPTPTSVPVIAMVPPTAVPTPTPTPTPPTPAPTDMPPPNPPTDVPILAA
                     ATDVPVLPTTPPVPTDAPVLPTTPPVPTDVQAPPPTDS"
BASE COUNT          446 a          997 c          976 g          536 t
ORIGIN      
        1 cggtcgcctg gagctacgat ctcctgcctc ccgcggagca atcggccttc cgggagctct
       61 cggtcttcac cggcgggttc acgatcgaga cggctgcggc ggtgctcgac gcggagtcat
      121 cttccccggg cgaggttccg gtgggggtgc tcgatctcgt ggcctcgttg ctcgacaaga
      181 gcctcctgag gaaacgcgaa ggatcgggca ggaccgagcc acgactttcc atgctcgtga
      241 ccgtcggaga gtacggtcac gagcagctcc gcgcgatggg cgaatacgag tctgctcacg
      301 atcgccatgc gcgctgggct ctagcctttg cggagacctc ggcgcgccat ctcgcggggc
      361 cggacgcctc ccgttggctt cacgagcttg atgacgagca gggaaacatc aggctcgcct
      421 tccgctggct cgacgcgtcc ggcgatcgca gtcgattcgt ccggctggcc ctcgccctgg
      481 aggattactg gctaacccgt gcccggtatt cggaggcgtt gcaatggctg gggcgggcga
      541 tggaactctc cgcggatgag cgcgacgttc cggatgctcg cctgcggtgc acgctcgcgg
      601 cggcctggat cgccctccgc ggcggcgacg ggatgcgggc gacggtgctc gctgacgccg
      661 gtctcgcgct cgcccgtgag aattccgacg ttcgcctcgt ggtccgcagc ctgaatgtcg
      721 ccgcgggtgt cgcccgtcgc gcggacgatc atcagcgggc gttcgacctg atcgaagaat
      781 ctcgccggcg gagtgaggcg gcgggcgatc gtcgcgcggt tgccgatgcg ctccgcgaat
      841 cggcatatgc cctgatgaat ctcgggcgaa tggaggaagc gatcgaagcc tgccgcgcca
      901 gcatcgccta cttcgaggaa gtcggaaaca cacagagtgc ggcgatggcg ctcgacaaca
      961 tgagcctcgt ctactacttg cggggtgatt acgcggaggc tcaccggtgc tcgcgggagg
     1021 cgatcgaggt cttccgccgc ctggggtacc cccgtggcgt cgggatcgcg ctctgccatc
     1081 tcggtctgag cgccacgcat ctcggcgatg tcgaggcggc ctggaacttt catctggaag
     1141 aactcgcgca tcgcgcgagt gtcggcgatc ctcgcggcta tgcggtctgg ctggaggccg
     1201 tcgcactcct gctcgcggtc tgtggtgacg gaaccggcgc gctgacgatc ctcggcgccg
     1261 ccgacgcggc acgcgaacgg gcgcaggcgc cgctcgcccg caccgagcgt ggcgaccgcg
     1321 atcacagcct ccgactggtt cgccgccagc ttacggacga cgcgatcgac gcggcgctgc
     1381 gtcaggggca atcgctcgcg atcccggccg cgatcgagcg agcctgcgcg ctcgttcccg
     1441 tcgcgattgc cgggtatcgc aagccggaac cgccttcggc gcggtcggcg ttcggtctca
     1501 gcgcgcgcga ggaagagatc ctccagctcg tggcgcaacg gctgagtgac cgcgagatcg
     1561 cggaggcgct cttcatcagt cgccacacgg ttgcccgcca cgttgccaac atcctgcgca
     1621 agctcgacgt cagctcccgc tatgacgccg cccgcatcgc gaacgagcac tgagcctcga
     1681 ccgggtggca gccgcagtgc ggacggagga gtgcgagccg ggacggggtt cgtcccaagg
     1741 cctctgcgga gctatacctt ccccatcccg tcctcacggc gcttgtcgcc gtttccgacc
     1801 tgggtgcaag gcgccgcgca ggaaagcagc aggcgggaca ttcctccgtc cagcgaccga
     1861 agatcgtgcg gccagcgctc ctgcggcagt cgctgcactc cgtggtcccg ccccacgtac
     1921 gaccgatgga cccgagccgc gtcccggagt gcgggaatcc tcccagacga ccgtctgact
     1981 cgaccgccgt cgtggaaaac cgcggtcgta cgtagggcgg gacgacgaaa tccagagacc
     2041 ggcattcagg gagtctctgg agcaaggcgc gcggtctgtg gatgaaggaa tgtcccgcct
     2101 ccccgttggc gatgaatgcc ttcggcacgt gaccgccgtc cccggcgtcc gttcgcgagc
     2161 ctgcccgggc gccgccgcgg ggcgagacac ggggcggcga cccatgtggt cgcctcgggc
     2221 ccggatcgcc gacgcgaggt ccgggagcgc gtcgcgcggg ctcgatatgc gttgtttcgc
     2281 ttatcgcctt atacgcttat caggttagta cttttatgta tttatggtac ttatggactt
     2341 atcttggagt ttgagaactg gcccgatagt gtttttgtat aggactaagg gttgtgtatc
     2401 ctgcggtcct gccgtatgat ccttctcggg acaccaaatt tatttatgcc gttcgggtcg
     2461 ctgtctggat gcttcggggc atccatagag acctgtcgtg tctggaatgt ttagcccata
     2521 aaggggaggg ggtcctatgg ccaccgcaaa cctggatgtc gtcatgcggc gcgtgcttcg
     2581 ctccggggcg ccggtaattc ggctcgccgt gattgccgcg atgtcgtgcg cgctcattct
     2641 cgcctcgtcc gtgatggcgc aaggcagcgt gactcccacg ccggtggcga cgttgacgcc
     2701 aacgccgacc tccgtgcccg tgatcgccat ggttccgccc accgcggtcc cgacgcccac
     2761 accaactccg accccgccca ctcccgctcc tactgacatg ccgccgccga atccaccgac
     2821 cgatgtcccg atcctggccg cggcaaccga tgttcccgtc ctgccgacga ctccgcctgt
     2881 gccaaccgat gctcccgtcc tgccaacgac tccgcccgtg ccaactgacg tccaggcgcc
     2941 gccacccaca gattc
//
LOCUS       DASTMD010000917         1964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2588432, whole genome shotgun sequence.
ACCESSION   DASTMD010000917 DASTMD010000000
VERSION     DASTMD010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2588432"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFQ54_13615"
     CDS             <1..207
                     /locus_tag="VFQ54_13615"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU0166080.1"
                     /translation="AFDRLGLHALKSWVIFQNTRSAAALRKQGYVEAGRSHWAYPIDG
                     GFGNAVVFTLHADTWRALPRTSAE"
     gene            241..1440
                     /locus_tag="VFQ54_13620"
     CDS             241..1440
                     /locus_tag="VFQ54_13620"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0166081.1"
                     /translation="MPDVAIEPKKIAGPALQTALVGDLVYLRPIYADDAATGTSWRQS
                     VFPESTSRVESWIEENLGKEGPEKKETLAIVRKSDDLVVGSIVLEHELSATIANVRVD
                     PIHGPAGLVWKGEAIVLVGRWVVDERHAVIMHVDVTANETSVIATIEAGGFRQSARFR
                     EMRFIDGRWVDLLMYDYANALWVARLGHPAEIPLERTGTGEPRPVPARVTLTDDPPRG
                     AILVGERVYLRAFETKDADESAVWGMREPETFHTRGRGAYSPAATLRELKEAEAKELP
                     TYIAFMVCLRGTDEFIGAVELFDVDPVHRNAETGSWFHKPEYRGGGYGSEAKQLLLGY
                     AFNHLRLHMVHSWVLFSNTRSAAALRKQGYKEAGRANWLYPAHGTLSNMVVFDLLADE
                     WRAMPRT"
     gene            1483..>1964
                     /locus_tag="VFQ54_13625"
     CDS             1483..>1964
                     /locus_tag="VFQ54_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166082.1"
                     /translation="MTETLSTTGGDTTVVKEPGSAHQTFLVGEEIYLRRVEKDDARLT
                     VSWRESLFPLAPSITESWITDDLTKAEQAWFVILRKADDRIVGSLRPARFGPYTELKA
                     YVDPFYGPDADRWRAEVYRLVLPWMVDEQARVGVHVSVAGDETATIAALLEIGAWEVQ
                     R"
BASE COUNT          344 a          626 c          639 g          355 t
ORIGIN      
        1 gcattcgatc gtctcgggct gcacgcgttg aagagctggg tgatcttcca gaatacgcgc
       61 tcggcggccg cactccgcaa gcagggctat gtcgaagccg ggcgaagcca ctgggcctat
      121 ccgatcgatg gaggcttcgg caatgccgtt gtcttcactc tccacgccga tacctggcga
      181 gcactgccgc gcacaagcgc cgagtaattc gccgcctgtc catccacgaa gaggtgattc
      241 gtgcctgacg ttgcaattga accaaagaag atcgccggtc cggcgctcca gaccgcgctc
      301 gtcggcgatc tcgtctacct gcgcccgatc tatgccgatg atgccgcgac cggcacctcc
      361 tggcggcagt cggtcttccc ggaatcgacc agccgcgtcg agtcctggat cgaggagaac
      421 ctgggcaagg aaggtcccga gaagaaggag acgctcgcca tcgtccgcaa gtcagacgat
      481 ctcgtcgtcg gctcgatcgt gctggagcac gagttgtcgg cgacgatcgc gaacgtccgc
      541 gtggacccga ttcatggccc tgcgggcctc gtctggaagg gtgaggcgat cgtgctggtc
      601 gggcgttggg tcgtcgacga gcgccacgcc gtgatcatgc acgtcgatgt caccgcgaac
      661 gagacgtccg tcatcgcgac gatcgaggct ggcggctttc gccagtctgc gcggttccgc
      721 gagatgcggt tcatcgatgg tcgttgggtc gacctcctca tgtacgacta tgcgaacgcg
      781 ctctgggtcg ccagactcgg gcatccggcc gagatcccgc tggagcgaac cgggaccggt
      841 gagccgcgtc ccgttcccgc ccgcgtcacg ctgacggacg atccgccgcg cggcgcgatc
      901 ctcgtcggcg agcgcgtcta cctccgtgcc ttcgagacga aggatgcgga cgagagcgcg
      961 gtctggggaa tgcgcgagcc ggagacgttc cacacccggg gacgtggcgc ctacagtccc
     1021 gccgcgacgc tgcgcgagct gaaagaggcg gaggcgaagg aacttccgac gtacatcgcc
     1081 ttcatggtct gtctccgagg gaccgatgag ttcatcggcg cggtcgagct cttcgatgtc
     1141 gaccccgtcc atcgcaatgc cgagaccggc tcctggttcc acaagccgga gtatcgtggt
     1201 ggtggctacg gctccgaggc gaagcagctg ttgctcggct atgccttcaa tcatcttcga
     1261 ttgcacatgg tgcattcctg ggtgctcttc agcaacacgc gttcggcggc ggcgctccgc
     1321 aaacagggat acaaggaggc gggccgtgcg aactggctct accccgctca cggcacgctc
     1381 agcaacatgg tggtcttcga tctcctggct gatgaatggc gggcgatgcc gcggacatag
     1441 acggcccgcg cattgcctga ccccgaaaag aatcgagaag ctatgaccga gacactatcg
     1501 acgaccggtg gcgacacgac ggtcgtaaag gaaccgggat ccgcgcatca gacctttctc
     1561 gtcggcgagg agatctatct tcgccgtgtc gagaaggacg atgccaggct gaccgtctcc
     1621 tggcgggaat cgctctttcc gcttgcccct tccatcaccg aatcgtggat cacggatgac
     1681 ctgacgaagg ccgagcaggc ctggttcgtc attctccgca aggcggacga tcgcatcgtc
     1741 gggtcgctcc gccccgcccg gttcggcccc tataccgagc tgaaggcgta cgtcgacccg
     1801 ttctacggac cagacgccga tcgctggagg gcggaagtct accggctcgt gctgccgtgg
     1861 atggtcgacg aacaggctcg cgtcggtgtc cacgtctcgg ttgccggtga cgagacggca
     1921 accatcgccg cgttgctcga gatcggcgcc tgggaggtcc agcg
//
LOCUS       DASTMD010000918         3747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_265231, whole genome shotgun sequence.
ACCESSION   DASTMD010000918 DASTMD010000000
VERSION     DASTMD010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265231"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFQ54_13630"
     CDS             <1..881
                     /locus_tag="VFQ54_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166083.1"
                     /translation="IAENDTSAQQTAAAQQQTIDSQQQTQEAVIENQNAVNARATEAA
                     NDEATRNALSASDRATQDAQSTQQAVESQATISAFATQQAEAANSNGDLNSQLATTEA
                     QATEQAASGVDTQGTIASLQGQIASNNNSLSTSQAQANSFQATSEALTQQQGNVQATI
                     SAAEQQAAANSLNPTAINLTVNVDLDGVNSNDASADNQAKQQLDSVFAPYVNGTNCTI
                     GFVLISSRSDELGEGVTLSDNIADLIQQEFPQLLATQSDGTKGLAHDSIALPGTSPAG
                     QVLLEVFVNQGCKPAT"
     gene            complement(1306..2463)
                     /locus_tag="VFQ54_13635"
     CDS             complement(1306..2463)
                     /locus_tag="VFQ54_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU0166084.1"
                     /translation="MTSPEFPLIAIDGNFLQMPGTGIATYLRCLHDALVANADAYGIR
                     VSLLEPGAGRLLKPGGRAQRLLWDAFGVTGKALATSPRPDLLHLPQFSAPFSSPMPMV
                     VTIHDVIPLVLPEYRASGAMRIYLRIMTRTVKKARRIVVPSESAAADVIRVLRIPAAK
                     VVVIPEAASPDLVPDQTGESREFVRQRFGIEGPYLFNIGGFDVRKNLPLLLQAFAQAW
                     PLFPAETTLAIAGAPQSDRAAMFPPLDPLIAKLGLGNHVRLVGRVSDDERRSLYQAAA
                     AYVTPSMYEGFGLTPLEAMACGVPAIVANRTSLPEVVGNTGIVVEPTVDAFAQAMVDL
                     LRDDARRANLGETSLARASEFTWEKAAAETVAVYRAVTTRSSGAGTTPAAG"
     gene            2742..3068
                     /gene="trxA"
                     /locus_tag="VFQ54_13640"
     CDS             2742..3068
                     /gene="trxA"
                     /locus_tag="VFQ54_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU0166085.1"
                     /translation="MSNTVAVTDSTYQAEVIDSDKPVLVDLWAPWCGPCRIVGPILEE
                     IALEDAARLKIVKVNVDENQQYAGQLGVFNIPTMVLYKNGQPVEKLVGSLPKKNILDR
                     LNPHLA"
     gene            3185..3511
                     /gene="trxA"
                     /locus_tag="VFQ54_13645"
     CDS             3185..3511
                     /gene="trxA"
                     /locus_tag="VFQ54_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU0166086.1"
                     /translation="MSNTVAVTDETYQAEVLDSDKPVLVDFWAEWCPPCRIVGPILEE
                     IAAEDADRLKIAKVNVDLDNKHAGQLGIFSIPTMILYKNGQPVEKIVGAFPKQGILDR
                     LAPHLG"
BASE COUNT          763 a         1241 c         1156 g          587 t
ORIGIN      
        1 agatcgcaga gaacgacacg agcgcccaac agaccgccgc cgcccaacag cagacgatcg
       61 actcccagca gcaaacgcag gaagccgtca tcgagaacca gaacgcggtg aacgcccggg
      121 cgaccgaagc agcgaacgac gaggcgacgc gcaacgcgct ctcggcatct gaccgcgcga
      181 cccaggacgc gcagtcgacg cagcaggccg tcgaatccca ggcgacgatc tccgccttcg
      241 cgacgcagca ggccgaagcc gcgaacagca atggcgacct gaacagccaa ctcgcgacga
      301 cagaggccca ggccaccgag caggccgcga gcggcgtcga cacccagggc acgatcgcct
      361 cgttgcaggg gcagatcgca tcgaacaaca actccctctc cacgagccag gcgcaggcga
      421 actcgtttca ggcaacgagc gaggcgctga cgcagcagca gggcaacgtc caggcgacca
      481 tctccgccgc cgagcaacaa gcggcggcca attccctcaa ccccactgct atcaatctca
      541 cggtcaatgt cgacctcgac ggggtcaact caaacgacgc gagtgcagat aaccaggcga
      601 agcagcaact cgattcggta ttcgcgccat atgtgaatgg gacgaactgt acgattggct
      661 tcgtgctgat atcttcccga tcggatgagc taggcgaggg cgtcaccttg tcggataaca
      721 tcgctgacct gatccaacag gagttccctc agcttcttgc cacacagtcc gatggaacca
      781 aggggctcgc gcacgactcc atcgcgctcc caggaacatc acccgctggg caggttctgc
      841 tcgaagtctt cgtcaatcaa gggtgcaaac ccgcaaccta agattgcgag gcgggacagg
      901 ccgtccggac tgcggtccgg cagcggcggt cccgccctac gaacgaccga cgctttgcgg
      961 aacgccgcgt ccgatccagc ggcatgcatg gcaatcgccc cggtcttcgc tgtgggctgg
     1021 cacatctgaa tatcgacgaa tgcgatccga agtccgggtt ggtagggcgg ggtttcatac
     1081 cccgccggaa cgccaacatc ggcacgtgcc gccttgcgtc agggcgcccc gatgacgaga
     1141 aggggtcggc ggcggcccgg aacggcgcca agcgccgtgg ggacggaaca cgcggggccg
     1201 ccagccacac cctggtcggc gcccgcccga tgaacgcgac agcgttgggt ttacgccccg
     1261 tcctgccaac cccgttccga gcagacaggc tgaaaggaac agcgcctacc ccgcggccgg
     1321 agtcgtcccg gcgccggaag atcgcgtcgt gacggcccgg taaacagcga ccgtttccgc
     1381 agccgccttc tcccacgtga actccgacgc acgggccagg ctcgtttccc ccaggttcgc
     1441 ccgccgcgcg tcgtcgcgga ggaggtcgac catcgcctgc gcgaaggcgt cgaccgtcgg
     1501 ctcgacgacg atccccgtgt tcccgaccac ctccggcagg ctcgtccggt tcgccacgat
     1561 cgccggcacg ccgcaggcca tcgcctccag gggtgtcagg ccgaatccct catacatcga
     1621 tggcgtcacg taagccgccg ccgcctggta gagcgaccgg cgctcgtcat cggagacgcg
     1681 gccgacgagg cggacgtggt tgccgagacc caacttcgcg atcagggggt ccagcggcgg
     1741 gaacatggcg gcgcggtcgg attgcggtgc cccggcgatc gccagtgtcg tctccgccgg
     1801 gaagagcggc cacgcctgcg cgaacgcctg gagcaggagc ggcaggttct tccggacatc
     1861 gaagccgccg atgttgaaga gatacggtcc ctcgatgccg aatcgctgcc ggacgaactc
     1921 acgcgactcg ccggtctgat ccgggacgag atcggggctc gccgcttcgg ggatgacgac
     1981 gactttcgct gctggaatcc tgaggacgcg aatcacgtcg gcggcggcgg attccgatgg
     2041 cacgacgatt cgccgagcct tcttcaccgt tcgcgtcatg atcctcaggt agatgcgcat
     2101 cgccccggat gcccggtact ccggaagcac gagcgggatc acgtcgtgga tcgtgacgac
     2161 catcggcatc gggctcgaga atggcgcgct gaactgggga aggtggagaa gatccggccg
     2221 cggcgacgtc gccagcgcct ttccggtgac accgaacgcg tcccacagca agcgttgggc
     2281 acgaccgccc ggtttcagca ggcgccccgc tcccggttcg agtaagctca cgcgaatacc
     2341 gtaggcatcg gcattcgcca ccagcgcgtc gtgcaggcac cgaaggtagg tggcgatacc
     2401 cgttccaggc atctgcagga agttgccatc gatggcgatc agcggaaatt cgggactcgt
     2461 catctgttcg gacaactccg gttggttcga gtgcaggtcg gacatggcgc cacgggacat
     2521 gcgggttgga cggtcgggac tcccgatctc gcaaccccga caacaccgga tcgcggactc
     2581 cgcaggatac tacgatcgac tgcgaccggc ggaaatcctg atcccgacgc cgggtctgac
     2641 atccatccgg agaatgccat ggctggacac gtattcacgg cttgcacgct atggtatcat
     2701 tttggaagta atagcttacg tagctgattc aggagcaatt catgagcaac acagtcgccg
     2761 tcaccgacag cacctaccag gctgaggtga tcgattccga caagccagtg ctggtcgatt
     2821 tatgggcccc atggtgcggc ccgtgtcgta tcgttggccc gattctcgag gagatcgcgc
     2881 tggaggatgc cgctcgtctc aagatcgtga aggtcaacgt cgacgagaac cagcagtacg
     2941 ctggtcagct cggtgtcttc aacatcccga cgatggtcct ctacaagaac ggccagccgg
     3001 tcgagaagct cgtcggctcg ctgccgaaga agaacatcct cgatcgcctc aatccgcacc
     3061 tcgcctgatc cagtcgcccc atctccgcga cgaaacgaag gacccggtcg tcgccgaccg
     3121 ggtcttgtcg tatccggcag gtcatgacaa gcagccgcgc tcatagcgca ggcaggagca
     3181 caccatgagc aacaccgtcg ccgttaccga cgagacctac caggcagagg tcctcgattc
     3241 cgataagccg gtcctcgtcg acttctgggc cgagtggtgt ccaccatgcc gtatcgtggg
     3301 cccgatcctc gaagagatcg ccgccgagga cgccgatcgg ctcaagattg ccaaggtcaa
     3361 tgtcgacctc gacaacaagc acgccggtca gctcggcatc ttcagtatcc cgacgatgat
     3421 cctctacaag aatggccaac ccgtcgagaa gatcgtgggc gcgtttccaa agcagggaat
     3481 cctcgatcgt ctcgcgcccc atctcgggta atcgccagaa cgccacaaac gaagggggac
     3541 ccgatctctg acgagatcgg gtccctcttc gtgtccgcgc gaaagagctc aggcgctttc
     3601 cttggtcgac gggagcgtta tcgccggact caggccctcg agctcgtgcc gaatgtcatc
     3661 gacatccgca aactcgcgat agaccgaggc aaagcgaatg taggcgacgt ggtcgatatc
     3721 cttcaggccg cggagcgcga gttcgcc
//
LOCUS       DASTMD010000919         1647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1605174, whole genome shotgun sequence.
ACCESSION   DASTMD010000919 DASTMD010000000
VERSION     DASTMD010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605174"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..544)
                     /locus_tag="VFQ54_13650"
                     /pseudo
     CDS             complement(216..544)
                     /locus_tag="VFQ54_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611738.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(549..1647)
                     /locus_tag="VFQ54_13655"
                     /pseudo
     CDS             complement(549..1647)
                     /locus_tag="VFQ54_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160158.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          266 a          531 c          521 g          329 t
ORIGIN      
        1 attcttccaa gatgtacgaa atgcctgcag atttcgtctc aagatttcct cactctcttt
       61 caacgtgtac ctcagtaggc gcaacgagag aggttctcaa gtcagtgccc cccaaatcac
      121 ttcacggtag ccctggtcat caaggtagtc aagagtccga catgcctcat gttctttgag
      181 cgcgtgggtg aaccgtcccc ggttctttgc cgctcctatg cgggtgccag caccgggtgg
      241 gatggcgttt cgtggccatc gtagtacgcg gcctcgaact catcgggcgg gactcgccga
      301 tggtcgagtg cagacgccgc gcgttgtacc aatcgaccca tcctgcggtg acccattcga
      361 cgtagtagat cgtgcgcagc ggtcccgttc ggaaggatga gccgtcgcgg atcgcttcgt
      421 tcttgaatag cccgatcgtg gactccgcga gagcgttatc gtaggcgtcg ccgatcgagc
      481 cgatcgacgc ggcgatgccc tccagggcga gtgtctcagc gaacgacacg ctcgtgaatt
      541 gggcgagttc aaccggtcgt tgcaacaccg ggctgttgaa gtgagcgtat ctgctcctcg
      601 aatacttcgg tggggggtct tccaatcgag gctcttgcgc ggcctgttgt tgagcgcgag
      661 agcgaccgct tcgaggtctt cgtcggacca ccgtgagagg ccggtgcctt tcgggaagta
      721 cagacgcagc agcccgttcg tgttctcgtt cgtcggccgc tgccagggcg agtgggggtc
      781 cgcgaagaac accctcgtgc ccgtctcgag cgcgaactgg gcgtgcgcgg agagctcttt
      841 gccgcgatcc caggtgagcg tcttgcggag ttgctcggga agcttcgtca acgacgcgat
      901 caacgcagcg ttcatcgcga tggcgccgta gccgctcagt gcgggaccgt tcttgacggg
      961 cgggatctcg ccgtagcctt ccagcctcgg cagatgcacc agcagggtcc agcggctctt
     1021 gcgttcgacg atcatgccga tcgcggaccg gcccgtaccg atgatcagat caccctccca
     1081 atgcccaggg acggcgcggt ccgcggcctc cgcagggcgt tcggagaaca cgacatccgc
     1141 ggtgacatgc cctcgcgggc ggttctgcgc tcgagcccgt ggccgccgaa gcgcgcggcc
     1201 ggttcgcagg caagtgacga gctcacgctt gagcgcgcca cggccctgga tgaacagcga
     1261 ctggtagatc gcctccgggc tgatgcgcat ggactcatca tccgggaagt cgaccttcag
     1321 ccggtgcgag atctgctccg gactccacgc ggtcacccac cgcctatcct gtcgatgcgg
     1381 cttgttcaac cccttccatg ccggcggttg cggcccgacg acgatgctgc cgtcgggtcg
     1441 gcgaacgttg caggcaaggc gctcctggac gtactcgcgc aacttgtcgt tagtcacgag
     1501 ccccgccacc ttcgggcgct tcgccgcttg ttgcgccttc cattgcgcaa ccaacgctcg
     1561 gtactccagc ttcccgccac gggtggccgc gttccggcgc agctcgcgag ataccgtccc
     1621 agggtcgcgt ccgatcctgc gggcgat
//
LOCUS       DASTMD010000920         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_356232, whole genome shotgun sequence.
ACCESSION   DASTMD010000920 DASTMD010000000
VERSION     DASTMD010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356232"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1268)
                     /gene="clpX"
                     /locus_tag="VFQ54_13660"
     CDS             complement(24..1268)
                     /gene="clpX"
                     /locus_tag="VFQ54_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU0166087.1"
                     /translation="MHYRCSFCNKGQEEVRRLIAGPNQVYICDECVQLCREIIEEEEP
                     AAPRTEPTLARIQTPKALYDQLNQYVVGQDQAKKILSVAVYNHYKRINAGLMNDDDVE
                     LQKSNILLVGPTGSGKTLLAQTLARLLDVPFSIADATALTEAGYVGEDVENILLRLIQ
                     NAGDVTRAQHGIIYIDEIDKIARKSDNPSITRDVSGEGVQQALLKIIEGTVANVPPQS
                     GRKHPHQEYIQIDTRNILFICGGAFEGLEEIVSKRVGKQSYMGFGGAVLPSMETENPL
                     SQVNHDDLLKYGLIPEFVGRLPVTVSLNHLTRPELIKILTEPRNAVVKQYQKFFALDD
                     VELVFTEDALEATARQAEERKTGARGLRTSIEEVLLEVMYEIPSLENVRKCIVDANVI
                     NNRSRPLLLTVNDHPIQYAQSA"
     gene            complement(1400..2044)
                     /locus_tag="VFQ54_13665"
     CDS             complement(1400..2044)
                     /locus_tag="VFQ54_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEU0166088.1"
                     /translation="MEIPKIDNLVPMVVESTNRGERAFDIYSRLLKDRIIFLGTPVDD
                     QVANLIIAQMLFLDHDDPEQDIRLYINSPGGAVYAGLAIYDTMRMVRANVQTFCMGMG
                     ASMAAVLLAAGEHGKRYALPNSRIMIHQGSGGFRGNIPDIEVQAKETFAVINTLTEIL
                     ATHSGQSFDKVKSDTQRDYYMTAAEAKEYGLIDEVLTPADLSVPLKVGANGSSL"
BASE COUNT          400 a          716 c          770 g          520 t
ORIGIN      
        1 tcgtgttgtg ctggcgcccg gatctacgcg ctctgggcgt actggatcgg gtgatcgttg
       61 accgtcagca gcagcggtcg cgagcggttg ttgatcacgt tcgcgtccac gatgcacttg
      121 cggacgttct ccagcgacgg aatctcgtac atcacctcga gcaagacttc ctcgatcgac
      181 gtgcgaagtc cacgggcgcc ggtcttgcgc tcttccgcct ggcgggcggt cgcttccagc
      241 gcgtcctccg tgaagacgag ttcgacgtcg tccagcgcga agaacttctg gtactgcttg
      301 acgacggcgt tgcgcggctc ggtcaggatc ttgatcagct ccggtcgcgt caggtgattc
      361 agcgagaccg tgaccgggag acggccgacg aactcgggga tcagaccgta cttcagcagg
      421 tcgtcgtgat tgacctggga gagcgggttc tccgtctcca tgctcggcag cacggcgcca
      481 ccgaagccca tgtagctctg cttgccaacg cgcttgctga cgatctcctc gagaccctcg
      541 aaggcgccgc cgcagatgaa gaggatgttc cgggtgtcga tctggatgta ttcctgatgc
      601 gggtgcttgc gcccactctg gggcggcacg ttcgcgaccg ttccctcgat gatcttgagg
      661 agcgcctgct gcacgccctc gccggacacg tcgcgcgtga tgctcgggtt gtccgacttg
      721 cgcgcgatct tgtcgatctc gtcgatgtag atgatcccgt gctgggcacg ggtcacatcc
      781 ccggcgttct ggatcaggcg gagcaggatg ttctcgacat cctcaccgac gtacccggcc
      841 tcggtcagcg ccgtggcgtc ggcaatcgag aaggggacgt cgagaaggcg ggcaagcgtc
      901 tgcgcgagca gcgtcttgcc ggatccggtc ggaccgacga gcaggatgtt gctcttctgg
      961 agctcgacat cgtcatcgtt catcagcccg gcgttgatcc gcttgtagtg gttgtagacc
     1021 gcgacggaga ggatcttctt cgcctggtcc tgccccacca cgtactggtt cagctggtcg
     1081 taaagcgcct tgggtgtctg gatccgggcg agcgtcggtt cggtgcgagg agctgccggc
     1141 tcctcctcct cgatgatctc gcggcaaagc tggacgcatt cgtcgcagat atacacctgg
     1201 ttgggaccgg caatcagccg ccgaacttct tcctgaccct tgttgcagaa tgagcaccga
     1261 taatgcaccc ggccacctcg tcgtttctct cttgtattgc gcgtagcctg tcgcagtccg
     1321 atgcgtggga gcgatgcagt tcaggttgtg ccacgcagtg cgaatgcggt tgaaggaatc
     1381 ctggttcgtg cggcggcggc tagaggctgc tgccgttcgc gccgaccttg agcggaacgc
     1441 tcaagtctgc cggggtcagg acttcatcga tcaggccata ttccttggcc tcggcggcgg
     1501 tcatgtagta gtcccgctgc gtgtccgact tgaccttgtc gaagctctgg ccggaatgcg
     1561 tggcgagaat ctccgtgagg gtatttatga cggcgaaggt ctccttcgcc tgcacctcga
     1621 tgtccgggat gttgccgcgg aatccaccgg aaccctggtg gatcatgatc cgcgagttcg
     1681 gaagcgcgta ccgcttgccg tgctcgccgg cggcgaggag gacggccgcc atgctggcgc
     1741 ccattcccat gcagaaggtc tggacattgg cgcgcaccat ccgcatcgtg tcgtagatgg
     1801 caagaccagc gtagacggcg ccacctggcg agttgatgta gagccggatg tcctgctcgg
     1861 gatcatcgtg atcgaggaag agcatctgcg cgatgatcag gttcgcaacc tggtcgtcaa
     1921 ccggagtgcc gaggaagatg atgcgatcct tgagcagacg agaatagata tcgaaggcgc
     1981 gctccccgcg gttggtgctt tcgaccacca tcgggaccag attgtcgatc ttggggattt
     2041 ccattcctgt atgacctttt caacgtggtg cggaccgctt gggccctaca aatggacggg
     2101 ctcggagcgg ggaatctgac cactcgctaa tggttcaatg atagtctgca tgtcgaggaa
     2161 aacacctccc caaagggacg atcctgactc acaggttggc ggattctacc acggtcctgc
     2221 acgcaccccg atcatcttga cgggtcggct tccgaatgtg gctagccctt ttcgtcgtct
     2281 gcggcaccgc tgcacggatt tcgcggaatc gggccgctat ggccgtggcg tcgcgacgtt
     2341 ctcgcacaca atcgcccacg gtttttcctg tactcgcgtc atcgcgaggg cgatcgggcg
     2401 ggagcc
//
LOCUS       DASTMD010000921         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_87682, whole genome shotgun sequence.
ACCESSION   DASTMD010000921 DASTMD010000000
VERSION     DASTMD010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87682"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFQ54_13670"
     CDS             complement(<1..625)
                     /locus_tag="VFQ54_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166089.1"
                     /translation="MRGVTLDKYIDGIRHDPGLLQDLLRRFVLLAGEMEDMSLAHGDL
                     SHGNILVDDGELRLVDYDGMYVPGMPFLTSAELGHDNYQHPYRDRSVFGPTTDRFSAI
                     VIYLSIRALMYRPALWERFSNGDNLIFVRDDFVDPANSALMAALDSVGQMKGLMSRFA
                     SLCASNMDDIPALSMFVGQASAADQRTNLRPAPPVILPQEIRAPQTRI"
     gene            complement(980..>1548)
                     /locus_tag="VFQ54_13675"
     CDS             complement(980..>1548)
                     /locus_tag="VFQ54_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166090.1"
                     /translation="YLQAPARDPSAAWLLPIANEYRQSFDLRELRWNLQEKAGRGGFA
                     TLLGGQIDLKQRILTCMAVGDTCLYIRHQGEIVPFPARFRDTTAFGDHPFLIATNPTY
                     NDNIMERRESMKARLPKGETIMYAMTDSLARWFAEGVGAGDAPWRRMDRVVGGGGREF
                     SRFIDAERQRGTLEDDDSTMLRIRILLK"
BASE COUNT          322 a          481 c          438 g          307 t
ORIGIN      
        1 tgatccgcgt ctgtggggct cgaatctcct ggggcaggat gacgggcgga gctggccgca
       61 agttcgttct ctgatcggca gcagatgcct gtcccacaaa catcgagagc gccgggatgt
      121 cgtccatgtt tgacgcgcag aggctggcga atcgtgacat gagacccttc atctgtccca
      181 cggagtcaag cgcagccatc agcgccgaat tcgccggatc cacaaagtca tcccggacaa
      241 agatcaggtt gtcgccgttc gagaatcgct cccagagcgc cggcctgtac atcagcgccc
      301 ggatcgagag atagatgacg atcgcggaga agcgatcggt cgtcggaccg aaaaccgagc
      361 gatcccggta cggatgctgg tagttgtcat gtcccagctc ggcgctcgtc aggaacggca
      421 tgccgggaac gtacatgccg tcgtagtcga cgagtctcag ttcaccgtcg tcaacgagaa
      481 tgttgccgtg gctgaggtcc ccgtgcgcga gcgacatgtc ctccatctcg ccggccagca
      541 agacgaatct ccgcagaagg tcctgaagca accccggatc gtggcgaatg ccatcaatgt
      601 acttgtcgag ggttacccct ctcacccagg acatcttcac gattggaaag cgttcgccct
      661 ggaccaggat gccggttggc cgatattcga acgaaacgag gaatttccgc tttgttcgat
      721 ccagaaaccc gcttatgtac tcatatctcg tctggaggtc gccaatatcc ttgtgaaagc
      781 agcggatagc ccattccctg tcgccgatcg tcagcttgta ggtcgccgca aaacccccgg
      841 agcgcacgcg gggcacgcca tttggccggc tctcgacgac cgctgtttga agctcgggat
      901 cagaaaaaga gaccgcaggg ttctgcaacg cctcttgata gagcgacaga ttgggaaggg
      961 ccatcgggtc gttgcttctc tacttgagga gaatgcgaat cctcaacatc gtcgaatcgt
     1021 catcctccaa tgtcccgcgc tgtcgctccg catcgatgaa gcgagaaaat tcgcgcccac
     1081 caccaccgac gacacggtcc atgcgacgcc acggcgcatc cccggcgccc acaccctccg
     1141 cgaaccaacg cgccaggctg tccgtcatcg catacatgat cgtctcgccc ttcgggaggc
     1201 gagccttcat ggattcacgt cgctccatta tattgtcgtt ataggttgga ttggtggcga
     1261 tgagaaacgg atgatctcca aacgccgtgg tatccctgaa ccgcgcggga aacgggacga
     1321 tctcgccctg gtgcctgatg taaaggcagg tatccccgac cgccatgcac gtcaggattc
     1381 gttgcttcag gtcgatctga cctcccagca acgtcgcgaa gcctcctctg cccgcctttt
     1441 cctggagatt ccaccgaagc tcgcgcagat cgaatgattg cctgtactcg ttcgcgatcg
     1501 gcagcaacca cgccgccgac gggtccctgg ccggagcttg caggtacc
//
LOCUS       DASTMD010000922         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_174732, whole genome shotgun sequence.
ACCESSION   DASTMD010000922 DASTMD010000000
VERSION     DASTMD010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174732"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..1210
                     /locus_tag="VFQ54_13680"
     CDS             449..1210
                     /locus_tag="VFQ54_13680"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU0166091.1"
                     /translation="MKVAIFSDVHGNSIALDAVLRDIAPEHVDAFWVIGDLVALGPDP
                     SGSARMLAALPDATFVRGNTDRYVVNGDVPGGPAQLEAARQDPERLPRYLRLFSNFPW
                     ARGAIAATGHYAWLAQIPLEIRVTLPDGTRVLLVHAAPGTDDGPGIPPQDVMSDDDLA
                     SVLAGADADLVIVGHTHVPLDRTVDGVTVHNLGSVSVPKTDEPRAMWTLLTADESGHT
                     LERRFVEYDVAKVHAALDAAHHPYADAIKEMIPLA"
     gene            1473..2306
                     /locus_tag="VFQ54_13685"
     CDS             1473..2306
                     /locus_tag="VFQ54_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166092.1"
                     /translation="MQTNRLNRRAFVTATAATSAIAVGLFATSAPATFAAQSATPIPV
                     TPLGFASTRLRTLTSASARDQVTAAVLSDFADQVKPLAGYEGYILGDVIDDPTKQVAI
                     TVLQNADQADAFNTTAATFVASVADLVDANATVTWAGDVLIDAGPSAAPAATPAASPA
                     ATPLPAATPIVTNSEGYLAVRIHTSKPDTDPRDIIPQIISGFLPIVTGLPGFKGYLWY
                     PIDGGFVAISLYDSVASANASNAAAQDWAVANVSDYTDGNPQIINANVVYVDLPIIPG
                     L"
BASE COUNT          473 a          896 c          847 g          455 t
ORIGIN      
        1 atcccggagc tggaccagcc cgataggaca ccagtgatta agatctatgc caagggaaag
       61 ggaccaggca atcctgacat cgtcaccctt cgcaccgagg cgtacatcac cggttgggag
      121 cccagaaaca agcgagccga gagctgagtc gggcacacca caaagccgct tcgcggcaaa
      181 tacggcatga aaatggaccg gaggtgttac acagcgcctg ccggctcatt tgctttccat
      241 gaccagcgtt tgaaccaggc gtggccgagc agatcatccg ggatgcgagg gatgtttcaa
      301 tcctcacccc cgtccggagg aagggcacaa cattaggaga tcgtgaccga gatgtgcggc
      361 ggatcccgtt tccaatgcgt cggaactcgc gaggctgggc gtacacttgc ctgatcgaag
      421 ctgcgaacca ggcgcgggag gtaatcgaat gaaggtcgcg atcttttcgg atgtccacgg
      481 gaattccatc gcgctcgatg ccgtcctgcg ggatatcgcc ccggagcacg tcgatgcctt
      541 ctgggtgatc ggtgacctcg tcgcgctcgg accggatccc tccggttccg cccggatgct
      601 cgcggcgctt cccgatgcga ccttcgtgcg cggcaacacc gatcgctacg tggtgaatgg
      661 tgacgttccc ggcggcccgg cccagctcga ggcggcgcgt caggatccgg aacggttgcc
      721 gcgctacctc cgcctcttct cgaatttccc ctgggcacgc ggggcgatcg cggcgaccgg
      781 tcactacgcg tggctcgcgc agatcccgct cgagatacgc gtgacgttgc cggatgggac
      841 gcgcgtcctc ctcgtccatg ccgcgccagg taccgacgac ggacccggca tcccgccgca
      901 ggatgtgatg agcgatgacg acctcgcgag cgtgctcgcg ggcgcggatg ccgatctcgt
      961 gatcgtcggg cacacgcacg tcccgctcga tcgcacggtc gacggcgtca ccgtccacaa
     1021 cctcggaagc gtcagcgtcc cgaagacgga cgagcctcgc gcgatgtgga cgctgctgac
     1081 ggcggacgaa tccggtcaca cgctcgagcg tcgcttcgtc gagtacgacg tcgcgaaggt
     1141 ccacgcggcg ctggatgccg cgcatcatcc atatgcggat gcgatcaagg agatgatccc
     1201 gctcgcctga ggcgcggccg tcgatgggcg cgatggaggg gaggttcggg caatgccggg
     1261 cctccctctt tgcatctgcc cagatcaaca atttttgcgt cactgcatgc ataatagagg
     1321 cgggttcttc atcaagggtg gttcgtcgcg cgtgggccga acgagccaga tcaggggtcc
     1381 tggatcgcaa tttgcgacgt cactcatccc gatcggcagg gcggagaccg agtccccgcc
     1441 tgtcgtccgt ttttccaccg cagggagaag acatgcagac gaatcgcctg aatcgccgcg
     1501 ccttcgttac ggcgaccgcc gccacgtcag ccatcgccgt gggtctcttc gcgacatcgg
     1561 ctcccgcgac gttcgcggcc cagagcgcga cgcccatccc ggtcacgccg ctcggcttcg
     1621 ccagcacgcg actccggaca ctcacctccg cctccgcgcg ggatcaggtg accgcggctg
     1681 tcctttccga cttcgccgac caggtgaagc cgctcgcggg atacgaaggc tacatcctgg
     1741 gcgatgtgat cgacgatccg acgaagcagg tcgcgatcac cgtgctccag aatgccgacc
     1801 aggcggatgc gttcaatacg acggctgcca ccttcgtcgc cagtgtcgcc gacctcgtcg
     1861 atgccaatgc gacggtgacg tgggcgggcg atgtcctgat cgatgccggg ccatccgccg
     1921 ccccggcggc aacgccggct gcctcaccgg ccgcgacgcc actaccggcg gcgacgccga
     1981 tcgtgacgaa cagcgagggg tatctcgccg ttcgcattca cacgagcaag ccggatacgg
     2041 atccgcggga catcatcccc cagatcatct ccggcttcct gccgatcgtg accgggctgc
     2101 ccggattcaa ggggtatctc tggtatccga tcgacggtgg cttcgtcgcg atctccctct
     2161 atgactcggt ggcttcggcg aacgcctcga acgccgccgc acaggactgg gcggtcgcca
     2221 acgtcagcga ctacacggat ggcaacccgc agatcatcaa tgccaacgtc gtctacgtcg
     2281 acctgccgat catcccgggg ctgtaagccc ctgcggtgca ttccgcgaca agccgtcccg
     2341 gtcatcacac gcgtcgtggg ggcgcctgac ggattccctt acggcgcaat ccggaagcga
     2401 agacgggctc tcagccaacc ggggtctggt gggctggagt ctgtctccaa cgctgtcgca
     2461 tagatcgagc ctgtcgagaa atccccctgt tcgtaggggc gagcctcgtg ctcgcccacc
     2521 gccccgttcg acgagctcag ggcgggctcc ggcggacatc cgcaggatct tcgacccggg
     2581 gcgcgctccc ggaagcgtct cggcagcgcc gcattgagcc ggaacggcga caagcgccgt
     2641 ggggacggca atggagcacg ctcatcgcca c
//
LOCUS       DASTMD010000923         1584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_266269, whole genome shotgun sequence.
ACCESSION   DASTMD010000923 DASTMD010000000
VERSION     DASTMD010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266269"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFQ54_13690"
     CDS             complement(<1..626)
                     /locus_tag="VFQ54_13690"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0166093.1"
                     /translation="MIYSFRTLLGYTQEGLSQAVQIEMSQQRNRKELGDYDSVSTNTI
                     TNWERKWLPTEKPRAPRFPTVRRVAMALRLKEGSPQYDALFEAWRVLQDDERPPSASR
                     PDDSRQEFISAGRDRDLTRLDHAIALAVSGSPQFLLVRGETGVGKTRLINETCRRAVD
                     THRKLVTIWGDCVALSGSSDLYHPFVQAIDLMTGGLPGSLFPQMISVT"
BASE COUNT          294 a          475 c          500 g          315 t
ORIGIN      
        1 ttcgtgacgg agatcatctg gggaaagagg cttcccggaa gtccgccggt catcaggtcg
       61 atcgcttgca cgaatggatg atagagatcc gacgatccgg agagggcgac gcaatcgccc
      121 cagatcgtga cgagctttcg atgcgtatcg acggcgcgac ggcaggtttc attgatcaat
      181 cgcgtcttcc cgaccccggt ctcgcctcga acgagaagga actgcggcga tcccgacacc
      241 gcgagggcga tcgcatgatc cagacgagtc aggtcccgat cgcgaccggc ggatatgaac
      301 tcctgccgcg aatcatccgg tcgtgacgcc gacggtggac gctcgtcgtc ctggaggacg
      361 cgccaggcct cgaagagggc atcgtattgg ggagatccct ctttcaacct gagtgccatc
      421 gcgacacgac ggactgtcgg gaagcggggt gcccgcggct tctcggtcgg cagccatttt
      481 cgttcccagt tggtgatcgt gttggtcgag acggagtcgt aatcgcccag ctccttgcga
      541 ttgcgttgct ggctcatctc gatctgaacc gcctgcgaaa ggccctcctg cgtgtagccg
      601 agcaacgtac gaaacgaata gatcaagctc ccgaactcgg tccgttctct ccgtgccatc
      661 ttgcctgccc caagcaagtc acctctgcga cgataacgtc ggggtggtgg ctcaaatgtg
      721 attgtgtgtc gactgagtcc ctcgagaatg attatgcccg atcgcggtgc cattttgtga
      781 tccatcggtg aagggttttc ggatggggga atccgacggc gagcgatggc gacccaaatg
      841 tcattcagtc gaaatgttgg gagcgattct gtgggaaaac tggggatttc cggagcggtg
      901 gcgggcctac catccaagac gtgagacgac gaggcgacga ggattccgat acctctccgt
      961 ccgccgagtc gacatcacct cgtctcgcgg tcgcgataga ccggtgtgtg cgagaccgac
     1021 cagaagtcat tcgggcgaag ttcgaattcc gttggaggaa cgaaaggggc gactccgcac
     1081 agatcacccg acgtcgcgag cggtgaggaa tctcccacct caccgtttcg ccatgcccgc
     1141 tgcgtggcag ctcgtcgaat cgatctccgg aattccggag aaacgtccag tgtcgcttcc
     1201 cggcgaattg ggggaagagt ggggattggg gacgggatcg cagccgtacg atgttggcac
     1261 gggagggatt cctggtggcg gcccgaacca agactgcgga gttctccctc aatgctggtt
     1321 gcctgcacgg gcgatcgcgt gagaagcggg tgggaaagcc tgtcccagca tcgccacccg
     1381 ctctctcatc gttccgccaa ccgtgatacc gggccgagac tccgccggtg tggccggcgt
     1441 cgctccccgc gtttcgtcca gtgtccaaac ctcatacctc gccgatgacc gcacatgccc
     1501 cgtgcgcgtc gtgctggctg gctttgcgtg tccgccgccg ctcctccttg ccgggcgcta
     1561 taatgcgcga cgtccgtcca gacc
//
LOCUS       DASTMD010000924         1864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_175133, whole genome shotgun sequence.
ACCESSION   DASTMD010000924 DASTMD010000000
VERSION     DASTMD010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175133"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFQ54_13695"
     CDS             complement(<1..579)
                     /locus_tag="VFQ54_13695"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU0166094.1"
                     /translation="MPKYIFVTGGVASSVGKGITTASLGRVIKSRGSSVSIMKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLSRASNVTTGQVYSAVIAKE
                     RRGDYLGGTIQVIPHITNEIKSRVRLASRQHESDVVIVEVGGTVGDIEGQAYLESIRQ
                     MRREVGRENCLIIHVTLLPYIGAAGELKTKPTQ"
     gene            complement(697..1353)
                     /locus_tag="VFQ54_13700"
     CDS             complement(697..1353)
                     /locus_tag="VFQ54_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0166095.1"
                     /translation="MAEIRWFGHGCFRIRAKEATIITDPVSRTTGYVLPKQTADIITI
                     SHGHEGHNNLAAIKPPFQVIDGPGEYEMHDVSVIGVRTFHDNAKGANFGHNTMYVIDV
                     EGLRFAHLGDLGHDLTEEQAEALDGVDILFVPAGGGNVLSPDHAAEMAARIGPKAVIP
                     MQYRTAKGDLSLAEVSTFCKQLGVDIPAAEPKLTVRAGDLGDALQLFLLEPESESGSR
                     "
     gene            complement(1681..>1864)
                     /locus_tag="VFQ54_13705"
     CDS             complement(1681..>1864)
                     /locus_tag="VFQ54_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166096.1"
                     /translation="NARQDRSDSPQMRDRTILVIARYVGRMRLQGDVIPFPSRRPMRS
                     PPRGMTQLNEKSQGSV"
BASE COUNT          363 a          534 c          598 g          369 t
ORIGIN      
        1 ctgcgtcggc ttcgtcttga gctcgccggc ggcgccgatg tatgggagca gggtgacgtg
       61 gatgatcagg cagttctcgc gcccgacctc gcgtcgcatc tggcggatcg attcgagata
      121 ggcctgaccc tcgatgtcgc cgacggtgcc accgacctcg acgatcacga catccgattc
      181 atgctggcgg gaggccagcc gaacgcgact cttgatctcg ttcgtgatgt ggggaatgac
      241 ctggatcgtg ccgccgaggt agtcgccgcg gcgctccttg gcgatgaccg cggaatagac
      301 ctgaccagtc gtgacattcg aggcgcggga gagattctcg tcgatgaagc gctcgtagtg
      361 tccgaggtcg aggtcagttt cagcgccgtc gtcggtcacg aagacttccc catgctgata
      421 tggcgacatc gtgccggggt cgacattcag atagggatcc agcttcatga tcgagacact
      481 ggatccgcgg ctcttgatga cgcggccgag tgatgccgtg gtgatgcctt ttccaacgga
      541 agacgcaact ccacctgtca caaaaatgta ctttggcatc ctgatcctcc cgggagccga
      601 tttcactatt ccaaccgacc ggcaacgaat ggcacgaatg ggtgtggtct gcggtgaggg
      661 cacggcgatc gcatcgcgta cccaggacga ggtcctttac cgggaaccag actcactttc
      721 cggctcgagc aagaagagtt gcagtgcgtc accgagatcg cccgcgcgga cggtcaactt
      781 cggttcggcg gcgggaatat cgacaccgag ctgcttgcag aaggtgctga cttctgccag
      841 ggagagatct cccttcgcgg ttcgatactg catcgggatg accgctttgg ggccgattcg
      901 ggcagccatt tccgcggcat ggtcggggga gaggacattg ccgcccccgg cgggaacgaa
      961 gaggatgtcg acaccgtcga gcgcctcggc ctgctcctcg gtgaggtcgt ggccgagatc
     1021 gccgaggtgg gcgaaacgaa gaccttcgac gtcgatcaca tacatggtgt tgtggccgaa
     1081 attggcgccc ttggcattgt cgtggaaggt gcggacgccg atgaccgaga catcgtgcat
     1141 ctcatattca cctggtccat cgatgacctg gaacggcggc ttgatcgcgg cgagattgtt
     1201 gtgcccctcg tgaccgtggg agatcgtgat gatgtctgcc gtctgcttgg gcaggacgta
     1261 gccagtggtg cgggagacag gatcggtgat gatcgtcgcc tccttcgcgc gaatgcggaa
     1321 gcagccatgt ccaaaccagc gaatctcagc cattgacccg tacaagctcc tcgtgaacgc
     1381 gcaacgtcgc caggatatgg cgaatgattc tggaaaaagc aaaaaccaaa cccagagcat
     1441 gcggggttac cacggatgcc gagttcaggt cgtcggaacg attcaattca aggggtttgg
     1501 gaaaggaatt tggcgacgaa cgacgtgcgt cttcccgcag cctccttcac tttaccacaa
     1561 cctttcgcag gatgcgatcc ccaaggcgcg caaatgttct agcgggattc gggtgcccgc
     1621 gatcgcgagt cgacatggat atcgccagcg acgaaggcgg cctgcggccg cggtccagcg
     1681 tcagactgac ccctgcgatt tctcgttcag ctgcgtcatt ccccggggag gcgaccgcat
     1741 gggtcgccgg gatgggaatg gaatcacgtc gccctgaagg cgcatcctgc caacgtaccg
     1801 ggcgatcacc aggatcgtcc ggtcgcgcat ctgcggggag tcacttcgat cctgacgggc
     1861 gtta
//
LOCUS       DASTMD010000925         1615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_175151, whole genome shotgun sequence.
ACCESSION   DASTMD010000925 DASTMD010000000
VERSION     DASTMD010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175151"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VFQ54_13710"
     CDS             <1..714
                     /locus_tag="VFQ54_13710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0166097.1"
                     /translation="YALAVLVGNPPEERSDLLRDETRPSAKVPIFVAQDPKALLRRRP
                     DVAAAERRVAAATASIGVTTAELFPSVTLIGNVGLEAGRSNDLTHGNSVKYIAGPTLT
                     WNILDFGRIRSRIEQADARNDRALAAYEQVILKALQEAESALVAVDRVDQQAAALAAS
                     TDAARDAAHLAQLRFEAGATDLLDLLDAQRQVLDAEDRLTRVETAREAASVNLYKAMG
                     GGWSNNETSAAPIARTTTD"
     gene            complement(723..1028)
                     /locus_tag="VFQ54_13715"
     CDS             complement(723..1028)
                     /locus_tag="VFQ54_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HEU0166098.1"
                     /translation="MSNHVLVVGTLTSKATAAGVDAVLRTEVEDTVRLYLRGKLDQWF
                     FKPDDNGVVFIFNATDIKEVDAVLAALPLSRGGLMEFRTTRLAPLRPLGVLLAEPAR"
     gene            complement(1205..>1615)
                     /locus_tag="VFQ54_13720"
     CDS             complement(1205..>1615)
                     /locus_tag="VFQ54_13720"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0166099.1"
                     /translation="LAMTRAFRTTLASRRGGAIVNMLSMAALVSLPVTGTYSASKAAF
                     LSLTRSMRAELMAQGTIVVGVLAVQTETAIGLRLPEPRMKPEEVVADALDAVQGGIND
                     EIAAGSLTRTAYQAFLADPKAFQARMSTRLPLPA"
BASE COUNT          303 a          511 c          534 g          267 t
ORIGIN      
        1 tatgccctgg cggtcctcgt cgggaatccg cccgaagagc gatcagatct tttgcgagac
       61 gagacacggc cgtcggcgaa ggttccgatc ttcgtcgcgc aggacccgaa ggcactgcta
      121 cgacgcagac ccgacgtcgc cgccgcagaa cgtcgcgtcg ctgccgcaac cgccagcatc
      181 ggggtcacca ctgcggaact gtttccctcg gtgacgttga tcggaaacgt cggcctcgag
      241 gcagggcgga gtaacgacct gacgcacggg aactcggtca aatacatcgc cgggccgacc
      301 ttgacctgga acattctcga cttcggacgg attcgcagtc ggatcgagca ggcggacgcg
      361 aggaacgatc gcgcgctggc tgcctacgag caggtgatcc tcaaagcgtt gcaggaggcc
      421 gaaagcgcgc tcgtcgcggt cgaccgcgtg gatcaacaag cagccgcgct tgcggcgtcc
      481 accgatgcgg cgcgtgacgc ggcgcatctg gcgcagctac ggttcgaggc aggtgcaacg
      541 gacctgctcg atctgctcga tgcacagcgg caggttctcg acgccgagga ccgtctcacg
      601 cgggtagaga cggcccggga ggccgcctct gtcaatctgt acaaggcaat gggcggcggc
      661 tggtcgaaca acgagaccag cgccgcgccg atcgcacgca cgacaaccga ctgacctcgt
      721 cgttatcttg ctggctccgc aaggaggacg cccagtggac gcagcggtgc cagtcgcgtg
      781 gtcctgaact ccatgagacc acctcggctg agcggcaggg ccgccaatac ggcgtcgacc
      841 tccttgatgt cggtggcatt gaagatgaag accacgccat tgtcgtcagg cttgaagaac
      901 cactggtcca actttcccct gagatagagg cgaacggtgt cctcgacctc cgttcgcagg
      961 acggcgtcga cgccggctgc cgtcgccttc gatgtcagcg tgccgacgac gagaacgtgg
     1021 ttcgacaaga cgggtgcagc gtgtccctcg agggggtcca ctggcgtgct ggactgcgcc
     1081 tgcacggatg ctgagagtac gcacgccagg acggtcgacg agagggcgat acgcgctcgg
     1141 tgactgtgac ggaatactgg catcgaaggc atggtcatgg agatctcgag aggtcgtgga
     1201 aggatcatgc cggcagaggt agtcgtgtcg acatccgtgc ctggaacgcc ttggggtccg
     1261 ccaggaaagc ctgataggcg gtgcgggtga ggcttccagc tgcaatctcg tcgttgattc
     1321 ctccctgcac ggcgtccagc gcatcggcca cgacttcctc gggcttcatc ctcggctcgg
     1381 gcaggcgcaa gccgatcgcc gtctccgtct gtaccgccag aacgcccacc acgatggtcc
     1441 cctgagccat caactcggca cgcatgctcc gggtgaggga caggaacgcg gctttcgatg
     1501 ccgagtacgt gcccgtgacc gggaggctca cgagcgccgc catcgagagc atgttgacga
     1561 tcgcaccgcc tcgacgactg gcgagcgtcg tcctgaacgc acgcgtcatc gccag
//
LOCUS       DASTMD010000926         1573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1160240, whole genome shotgun sequence.
ACCESSION   DASTMD010000926 DASTMD010000000
VERSION     DASTMD010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160240"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /gene="dmeF"
                     /locus_tag="VFQ54_13725"
     CDS             <1..1307
                     /gene="dmeF"
                     /locus_tag="VFQ54_13725"
                     /inference="COORDINATES: protein motif:HMM:NF033827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HEU0166100.1"
                     /translation="EAGPAVTRIHGSKSMNEPAVLFGSHDHVFLGAGHEKNERKTWAV
                     IVLCAVMMAVEIVGGSLFGSLALVADGLHMSTHASAMLIAALAYRYARKHAADRRFVF
                     GTGKLGDLAGFTSAVILAMIALLIGYEAVARVVAPVPIHFAEAVPIAVVGLLVNIVSA
                     WLLSGDDHHHGHGHGHGTDESDEHGHGHDDETQLISVKEGQYALSVFEEGMPPVFRIA
                     RLDQGVNPGENSVTVTTMRPDGMRQIFHFADRGRYLESTTEIPEPHTFIAWVSISGEE
                     QRVEFEEHGHDHGSGVDSRDHNIRAAYVHVIADAAVSVLAIIGLLLAKAFGWLWMDPL
                     AGIVGALVIANWSYGLMRDTGGILLDICPSNAIEKKVRAAVENAGDKLVDLHIWRLGP
                     GHFGAVISVVTAVAQRNPAFYHSILRGFKGLSHITVEVHPRS"
BASE COUNT          302 a          467 c          463 g          341 t
ORIGIN      
        1 aggaggcagg gcctgccgtc acacgaattc acggtagcaa atcaatgaac gaaccagcgg
       61 ttttgtttgg cagccacgat catgtcttct tgggtgccgg tcatgaaaag aacgagcgca
      121 agacttgggc tgtcatcgtc ctgtgcgcgg tcatgatggc ggtcgaaatc gtcggcggca
      181 gcctcttcgg ctccctcgcc ctcgtggccg acggtttgca catgtcgacg cacgccagcg
      241 ccatgctgat cgctgcgctc gcctatcgtt acgcccgtaa gcatgcggcc gatcgtcgct
      301 tcgtttttgg gacgggcaag ctgggcgatc ttgcgggatt taccagtgcc gtgatcctcg
      361 ccatgatcgc actgttgatc ggctacgaag ccgttgcccg ggtggtcgct ccggtgccca
      421 ttcacttcgc agaggccgtc ccgatcgccg tcgtcggtct cctggtgaat atcgtgagcg
      481 cgtggctcct gagcggcgac gatcaccatc acggacatgg ccacggtcat ggaaccgatg
      541 aatcggacga gcacgggcac gggcacgacg acgaaacaca actcatatcg gtcaaggagg
      601 gtcaatatgc gttgtccgta ttcgaagagg gcatgccgcc cgtgtttcgg attgcccggc
      661 tcgaccaggg tgtaaatccc ggcgagaact cagtgacggt gacgacgatg cgtcccgacg
      721 gtatgcgtca aatctttcat tttgcggatc gtggccgtta cctcgagtcc acgacggaga
      781 tcccggaacc ccacacgttc atcgcctggg tctcgatttc aggcgaggag cagcgcgttg
      841 aattcgagga gcacggccac gatcacggct ctggcgtcga ctcccgcgat cataatattc
      901 gcgccgccta tgtacacgta attgccgatg cggcggtatc ggtattggcc atcatcgggc
      961 tcctcttggc aaaggcattt ggttggcttt ggatggatcc cctcgcaggt atcgtcggag
     1021 cgctggtcat tgcgaattgg tcgtacggat tgatgcgcga taccggcggg attttgctcg
     1081 acatctgtcc gagcaatgcc attgagaaga aagtacgagc cgcggtggaa aacgccgggg
     1141 acaaactcgt ggacctccat atctggcgct taggtcccgg acatttcggc gcggtcatct
     1201 cggtggtgac cgcggtcgca caacgaaacc ctgctttcta tcactcgatt ctgcgcggat
     1261 tcaaaggcct ttcgcacatc acggtcgagg tccatcctcg gagttagccg gcggcatgag
     1321 cgacccggtg gggcgcagtg tagggtatcc ccgttgccga ctcgtagccg gtacagcgcc
     1381 gccaccctac ctcttatgtc agattgggag aacggtcaca cctcaccctc ccacgttacg
     1441 cgatcatcaa cccatagacc tcatgcgaat gcgctcgagc acttctatgc cccccgcgcg
     1501 ctcggcatga ccttcgagaa ctcggccacg ttcacgtttt tcctgacgcc gagagtagat
     1561 attcaatgaa tct
//
LOCUS       DASTMD010000927         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1963314, whole genome shotgun sequence.
ACCESSION   DASTMD010000927 DASTMD010000000
VERSION     DASTMD010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1963314"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFQ54_13730"
     CDS             <1..517
                     /locus_tag="VFQ54_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0166101.1"
                     /translation="FKGLVQSPTPTTVEGEYFQIRDAYLEVRSPKATGTPITIGGNGP
                     KLTLPLVAKYADEWNAVYPNLETYPELTARLNELLAAEGRQPSDVKRTAMSRLMIGRD
                     EAELATRIEPANLQSLKDRGAPVGTPDEIVQQLGAISELGIEGVMLQLLDMDDISALE
                     LFAEKVLPQLA"
     gene            complement(757..1602)
                     /locus_tag="VFQ54_13735"
     CDS             complement(757..1602)
                     /locus_tag="VFQ54_13735"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEU0166102.1"
                     /translation="MYRNSESDETWHHPLIRKADSRVTRRRLILGATGAVALVTFPGQ
                     LGQVAAQDATPSPVATPTFSPTGDADATALLISAAEAMAALSTFHFDLETVSGDSSVM
                     GLDVQKVTGDVKRPLDFQAEATVKVPFGSLTVKAVGLDGQFYIQNPLASDQKWTALDG
                     GSDLIAVVNPDVIILFAVRLLQDAKIDGSEKYAGVETTRVTGEVDFQKVAETVLGTSG
                     SSGGVTSQIAQGPVPLIIWISHDNLIQAIEIDGPLLAQEGADVTRLVSFDSFNDPVDI
                     EMPPL"
     gene            complement(1787..>2442)
                     /locus_tag="VFQ54_13740"
     CDS             complement(1787..>2442)
                     /locus_tag="VFQ54_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166103.1"
                     /translation="LLSFTGDIGNHWSVVDGIEFPIRVDLNLYPADASHFEELASWPN
                     APVSIDAMVLIDRTGQLRPQAEKIVGQSLAPLDMPERVDLVIAGFWYYAVRTWCKLQR
                     RPDWGVRFDISFMMHGNLMALLRLEAGSIARWRSADAAAGIEGDISPERLAALDDCIP
                     AANPEGLAVALASIVRLGALASAGAAGRYHRIWPEELAARMIALTGEPSFRAPGSGR"
BASE COUNT          471 a          788 c          762 g          421 t
ORIGIN      
        1 cttcaaggga ctcgtccagt cgccaacgcc gacaaccgtc gagggtgagt acttccagat
       61 ccgcgacgcc tatctcgaag tccgctcccc gaaggcgacg ggaacgccga tcacgatcgg
      121 tggcaacggc ccgaagttga cgctgccgct ggtggcgaag tacgcggacg agtggaacgc
      181 cgtctatccg aacctcgaga cctacccgga actcacggcg cgcctgaacg agcttcttgc
      241 cgcggagggc cgccagccat ccgatgtcaa gcgcaccgcg atgtcccgcc tgatgatcgg
      301 ccgcgacgag gccgaactcg ccacgcgcat cgagccggcg aacctgcagt ccctgaagga
      361 ccgcggcgct ccggtcggaa cgccggacga gatcgtccag caactcggcg ccatcagcga
      421 gctgggaatc gaaggcgtga tgcttcagct gctcgacatg gacgatatct cggccctgga
      481 actcttcgcg gagaaggtcc ttccgcaact cgcgtaagtc atctctctat tgcagcgaaa
      541 accgggacgg ttgcccgaca cgcttccgca tcgatcgcgg cgttccgggc agcggccggt
      601 cgttcgtagg gcgggactgc cgctaccgga ccgcagtccg gacggcctgt cccgcctcgt
      661 agatcgcccg gtgtgcgaca ggaacgcgaa cgcgcagacg gcccgggaat ccttcgattc
      721 ccgggccgtt cgcgtcgtta cgatgaatgt gaccgatcag agcggtggca tctcgatatc
      781 gacggggtcg ttgaaggaat cgaacgacac caggcgcgtg acgtcagcgc cctcctgagc
      841 caggagagga ccgtcgatct cgatcgcctg gatgagattg tcgtgactga tccagatgat
      901 cagcggcacc ggtccctgtg caatctgact cgtcacgcct ccgctcgatc ccgaggttcc
      961 gagcaccgtc tcggcgacct tctggaaatc cacctcgccg gtgacccggg tcgtctcgac
     1021 gccggcgtat ttctcgctac cgtcgatctt cgcatcctgg agcaggcgga cggcaaacag
     1081 gatgatgacg tcgggattga cgacggcgat gagatcgctt cctccatcga gcgccgtcca
     1141 tttctggtcg gaagcaagtg gattctggat gtagaactga ccgtcgagcc caaccgcctt
     1201 cacggtcagc gagccgaacg gaaccttcac ggtcgcttcc gcctgaaaat cgagcgggcg
     1261 cttcacatcg ccggtgacct tctgcacatc gagccccatc accgaactgt cgccactgac
     1321 ggtctcgaga tcgaagtgga atgtgctcag tgcggccatg gcctcggccg ccgaaatcag
     1381 caacgccgtc gcatcggcgt caccggttgg cgagaacgtg ggcgtggcaa cgggcgaggg
     1441 agttgcgtcc tgggcggcca cctgccccag ttgtccgggg aaggtcacca gcgcgaccgc
     1501 tccggtcgcc ccaaggatca aacgacgacg agtcacgcgg ctgtcggcct tgcggatgag
     1561 tgggtgatgc cacgtctcgt ccgattcaga attcctgtac acgtacgtcc ccttcctggg
     1621 atgcgggatt gggatcgaac atcattggtc gccccgtccg gacgaccgac aaggggccat
     1681 tgcggcgccc gtcgttgtcc atacgtcaat gctagggcca cgagatgaat ggttggtgaa
     1741 aggaccgtca ccgaagtgtg aagattctgc gaccagcgcc tgcaattcac ctgccagatc
     1801 ccggcgcccg gaacgagggt tcaccggtca gcgcgatcat tcgcgcggcg agttcttccg
     1861 gccatattcg atgataacgc ccggcggccc cagcggaagc caatgcgccc aatctgacga
     1921 tgctcgccag ggcgaccgcg agtccttcag ggtttgccgc cggaatgcag tcgtcgagtg
     1981 ccgcgagtcg ctccggactg atgtcgcctt cgatccccgc cgcggcatcg gcggatcgcc
     2041 agcgcgcgat cgaccccgct tcgagccgca gcaacgccat gagattaccg tgcatcatga
     2101 aggagatgtc gaagcggacg ccccaatccg gacgacgctg caatttgcac caggtgcgaa
     2161 cggcgtagta ccagaatccg gcgatcacga ggtcgacgcg ttccggcatg tccaacggcg
     2221 cgagcgactg gccaacgatc ttctccgctt gcggtcgaag ctgaccggtc ctgtcgatca
     2281 ggaccatcgc gtcgatcgaa accggggcgt tcggccagct cgcgagctcc tcgaaatgtg
     2341 acgcgtcggc gggatagaga ttgagatcca cacgaatcgg gaactcgatc ccgtcgacaa
     2401 cggaccagtg attcccgatg tcacccgtga aggagagcag ga
//
LOCUS       DASTMD010000928         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_357069, whole genome shotgun sequence.
ACCESSION   DASTMD010000928 DASTMD010000000
VERSION     DASTMD010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357069"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..395
                     /locus_tag="VFQ54_13745"
     CDS             201..395
                     /locus_tag="VFQ54_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166104.1"
                     /translation="MLELGRILTALGLVVASVGLVVYAMGQSFYDPASFEMNLGIVMM
                     IVGVVGAVVGVVLYRQTTED"
     gene            complement(488..2056)
                     /locus_tag="VFQ54_13750"
     CDS             complement(488..2056)
                     /locus_tag="VFQ54_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0166105.1"
                     /translation="MVPTRDQPSSDERTIALARLVFDLTQRFNGFDEFEDICAAVSDG
                     LATMVDYDRLAIVLFRGEGRDFEMRYSRGAAMEFLSGRPSELARQERLFDLVDPALFD
                     ITETPRFAGDVDRFAHGYRQAAVAPVVLDRENVGLFTLMSKTERKWNDSDLWILSSLA
                     GALGIILAGTSYRHEAEQRLREAEFIAELGLLLSFNCQLDALLDGAVEKISTAFAAPV
                     SLYLVQQDRATLRSASRLGFDDGRISRVLAQGIVDFERNPVASALTRGIDGGVLVSST
                     DADESLEMNEVLADELRQHEVRRVLTGPIAWGGRVLGALMIFQLPDPADGSVVIPPPR
                     QQQMALRVVQSLGPAIQNVLLQDDLTRALNESEVLRRLLSDTANRDDSGEALEIVVRA
                     AQLLYAADFVAIGRSEGDETHWLRMVGSRVPFDGERENRTPPPVADAMERLAPILVRD
                     YPIDPPMEPDSYPIHEVEGLRSSLAMPFAVEEQVKGVLMIGFRRPHFFDAPDIRFARS
                     LAHGVAASLLRHHG"
     gene            complement(2022..>2694)
                     /locus_tag="VFQ54_13755"
     CDS             complement(2022..>2694)
                     /locus_tag="VFQ54_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEU0166106.1"
                     /translation="IGLGGTFQARNGATAVAAVETLAANGFAIPDDAYAAGLANTRIP
                     GRIELIQDRVPVLLDGAHNPEKVAALALDVPRMLPTPEAGRRIAVVGVLEAKQADEMF
                     RSLVPVVDVIVATSPQVLAKESKHADQIEAIARNAGFSGPVFVDPEPLRAVDIAFAQV
                     REGAGDAILVTGSLYLVGNIRARWYDEESIVLQQTSWPTALGMALNVAEEHVDGANPG
                     SAFER"
BASE COUNT          503 a          901 c          838 g          452 t
ORIGIN      
        1 gctttgctct gtccgatatc cggtattgtc gcgcggagtt ccgccggacc tctcgacgag
       61 ttccgccgtt tccggtagtc ttgcgacgaa tgaggggtcc acgcttgggc ctggagccgc
      121 cggtcatggc gacgcaccgg ggaccgacgc agaaccgatc gcaccgaaac agagatttct
      181 ttcaggggga gacaatcgcg atgttggaac tgggacgaat tctcaccgca ttggggttgg
      241 tggtggcgtc cgtcggtctc gtcgtctatg cgatggggca gtcgttctac gacccggcca
      301 gcttcgaaat gaatctcggg atcgtgatga tgatcgtggg cgtcgtcggg gcggtcgtcg
      361 gtgtcgtgct ctaccggcag accaccgagg attaagcctc cggaatcatg cctgccagaa
      421 cacatcggcc cggacgggat tgcaccgtcc gggccgtcgt cgtttccccg tggaatcaat
      481 ggcgccgcta cccgtggtgg cgcagcaacg acgcggcgac accatgcgcc agcgaccgcg
      541 cgaagcggat atccggagca tcgaagaagt ggggccggcg gaacccgatc atgaggacac
      601 ccttgacctg ctcctcgacg gcgaagggca tcgccaacga ggaacgcagg ccttcgacct
      661 cgtggatcgg ataggaatca ggctccatcg gcgggtcgat cggataatcc cggaccagga
      721 tcggggcaag cctttccatc gcgtcggcca cgggcggcgg cgttcgattc tcccgctcgc
      781 cgtcgaaggg cactcgcgat ccaaccatcc gcaaccagtg cgtttcgtcc ccttcacttc
      841 gcccgatcgc cacgaagtcc gccgcgtaga gaagctgggc ggcacgaacc acgatctcca
      901 gggcctcgcc cgaatcgtca cggttcgcgg tgtcgctgag aagccggcgg agcacttcac
      961 tctcgttgag cgcccgggtg agatcatcct gaagcagcac gttctggatc gccggcccca
     1021 gcgactggac gacacgtaac gccatctgct gctgtcgcgg cggagggatc acaactgacc
     1081 cgtcggcagg atcgggaagc tggaagatca tcaacgcgcc gaggacacgt ccgccccacg
     1141 cgatcgggcc tgtaagcacg cgtcgaacct cgtgctggcg aagttcgtcc gccagcacct
     1201 cgttcatttc gagcgattcg tcggcatcgg tcgacgacac caggacgcca ccatcgattc
     1261 cacgggtgag cgcgctcgcc acgggattgc gctcgaagtc gacgattccc tgggcgagga
     1321 cccggctgat ccggccgtcg tcgaacccga gccgcgacgc ggacctgagg gtcgcgcgat
     1381 cctgctggac gagatagagc gagaccggcg ccgcaaaggc cgtcgagatc ttctccaccg
     1441 cgccgtccaa cagcgcgtcc agctggcagt tgaacgagag cagcaagcca agctcggcga
     1501 tgaactcggc ctcgcgcagt cgctgttctg cctcgtggcg atagctcgta ccggcgagga
     1561 tgatgccgag cgcccccgcc agcgaactca ggatccacag atccgaatcg ttccacttgc
     1621 gctcggtctt cgacatcagc gtgaagagtc cgacgttctc ccgatcgagc acaaccggcg
     1681 cgacagccgc ctggcgatac ccgtgcgcga accggtcgac atcgccggcg aagcgtggcg
     1741 tctccgtgat atcgaagagc gccggatcga cgagatcgaa aaggcgctcc tgccgcgcga
     1801 gctcggacgg acgaccactg aggaactcca tcgccgcacc acggctgtac cgcatctcga
     1861 aatcacgccc ctccccacgg aagaggacga tcgcgagccg atcgtaatcc accatcgtcg
     1921 cgagcccgtc cgacaccgcc gcgcagatat cctcgaactc gtcgaatcca ttgaatcgct
     1981 gcgtcaggtc aaagacgaga cgggcaagag cgatcgtccg ctcatcgctc gaaggctgat
     2041 cccgggttgg caccatctac gtgttcctct gcgacgttca gcgccattcc gagcgccgtg
     2101 ggccacgatg tctgctgcag gacgatcgat tcctcgtcgt accaccgcgc acggatattt
     2161 ccgaccagat agagggaccc ggtgacgagt atggcatccc ctgcaccttc acgcacctgc
     2221 gcaaacgcga tatcgaccgc acgaagcggt tccggatcga cgaagaccgg cccggaaaag
     2281 ccagcattcc gcgcgatcgc ctcgatctgg tccgcatgtt tggactcttt ggcgaggacc
     2341 tgcggtgacg tcgccacgat cacgtccacc accggcacga gactgcgaaa catctcgtcc
     2401 gcctgtttcg cctccagcac gccgacgacg gcgatccggc ggccagcctc gggtgtcggc
     2461 agcatccgcg gcacgtcgag ggcaagggcg gcgacttttt ccggattgtg cgcgccgtcg
     2521 agcagcaccg gcacccgatc ctggatcagc tcgatccggc cgggaatccg ggtattcgcg
     2581 aggcccgcgg cgtaggcatc atccggaatc gcgaatccgt tggcggccag cgtctcgact
     2641 gccgcgacag ccgtcgcccc gttccgggcc tggaaggtcc cgcccaatcc gatc
//
LOCUS       DASTMD010000929         6205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_2767299, whole genome shotgun sequence.
ACCESSION   DASTMD010000929 DASTMD010000000
VERSION     DASTMD010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2767299"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFQ54_13760"
     CDS             complement(<1..253)
                     /locus_tag="VFQ54_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166107.1"
                     /translation="MDTIKDLPIVFNLEQALDLGISKRRIYDWRDAGGLETMGDGVFA
                     RADVDDDHVKQLATFVLRRPEATICLLSALEIHGLTDEIP"
     gene            complement(321..1160)
                     /locus_tag="VFQ54_13765"
     CDS             complement(321..1160)
                     /locus_tag="VFQ54_13765"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0166108.1"
                     /translation="MMTTSTSTTGIVPTRTAAVLGPLRITIATAVNIIRIKIQALTLP
                     FYVLTFLGLPFFELVLVALIYRDNHDLRNYAVVAGTGTALIFAMLFSGGENLEGERSR
                     GTLGGLFISPAPRISWLLGFQLSAALEAIPIAVVSVGLGVAIFATPLSVNVPALLLTL
                     VLLIVCMWGIGMMLSALGTAVRNANQISNMVFPILMPLSGTMFPISEMPLWLRIPARC
                     LPFGYGMQALVDAMTKNASIADLGGDLWPLLGFAIVLPILGARSFRRLDRYARLQGHL
                     DMV"
     gene            complement(1183..2028)
                     /locus_tag="VFQ54_13770"
     CDS             complement(1183..2028)
                     /locus_tag="VFQ54_13770"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0166109.1"
                     /translation="MSALPAAMREPARTSLLRTNPRVIAATARVYTLRFASNPIQLIR
                     IPLAPVLSLGGFVLAYNISGQTSVPDSQVIGFLIIGIFMTEAWSSTVWGSGNALQAER
                     NLGTISAIISAPASTAAVVIGYTLGSLLIGLPSIVSCTVVGLLAGGRFTIDDPLAALV
                     ALLTVYVSTVCIGMAFGGLFILSRNSNSLSNVLQLPIYLLAGFYVPRSVLPGWLRRVG
                     DVIPLSHAVDALRGAMLSGESLGDLRNPLLAALGTSAIFLVIGIWALGKADRAIRRSG
                     TLDIL"
     gene            complement(2025..3092)
                     /locus_tag="VFQ54_13775"
     CDS             complement(2025..3092)
                     /locus_tag="VFQ54_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0166110.1"
                     /translation="MSTPAALTTTPSASVDSPIIATDLRRSFRKRSGWFRSGDLVEAV
                     RGISFHVPQGTIFGMLGPNGAGKTTTIKMLSTLLVPTSGTAHINGFEITRDELRVRQQ
                     LGVLFGGDRGLYNQLSGWENLRYFGRLYGMDDAAIKSRASMLLERVRLLDRASERVES
                     YSRGMKQRLHIAKTLLHSPAVIILDEPTIGLDPAAAIDVRELIASLIPDHTILLTTHD
                     MHEADILCREIAIVDRGLIVAQGAPATLKAEADVDRQVIITLASSLDGRRDSVEASLR
                     RLPVVSDLRHELAEDGAPVLTLRCEETTSALDTALAVLREASAPVRGIQILEPSLEDA
                     FMHATGREFEQMGAGASGGQE"
     gene            3627..4385
                     /locus_tag="VFQ54_13780"
     CDS             3627..4385
                     /locus_tag="VFQ54_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEU0166111.1"
                     /translation="MSRAERLLQLMQLLRLYRYPVTGQRLADELGVSVRTLYRDIATL
                     QYQGAQIDGEPGLGYVLRPGFMLPPMMFSEEEIEALVLGSRWVASRADSELSLAARNA
                     LAKISAVLPADLRESLDASSLIVPPGDFVAAGDFVAAGDRELRSIREAIRTERRLRIT
                     YADFRGETTERVVWPFSLAFFDRVRVVSAWCELRNDFRAFRTDRIEHLAILGDRYPRR
                     RQALLREWRQREGISDPSRPAAPAGRPVSTPDKN"
     gene            4483..4857
                     /locus_tag="VFQ54_13785"
     CDS             4483..4857
                     /locus_tag="VFQ54_13785"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0166112.1"
                     /translation="MEPKMFMLYVSDMDASVALYHGVLGIEPDRPTPAFASFLLSSGL
                     RFDLVLGTAVDPAPTAGGGAIETCFLVDSRGDVERVHDLWRARGLPIALAPQDKPFGY
                     TLVGVDPDGHRLRVIGPSVDPS"
     gene            4932..5318
                     /locus_tag="VFQ54_13790"
     CDS             4932..5318
                     /locus_tag="VFQ54_13790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0166113.1"
                     /translation="MEPNFTILYVGDMPKSAAFYDALIQREPVEASANFRVYVLSTGA
                     RVALWQRDDVQPAATAPGGVEFCLAVTSPADVDAVAATWRERGIPIAQDPVLLEFGYS
                     FVGLDPDGHRLRVHALSSDPAGTAEA"
     gene            5349..5780
                     /locus_tag="VFQ54_13795"
     CDS             5349..5780
                     /locus_tag="VFQ54_13795"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0166114.1"
                     /translation="MSGDRIPVETERQVARMEPRYFVLHVASIETSIALYTDLLGTTP
                     QRPSPTFAAFPLGSGVMLELWQRSGIEPASVAPGGAVDITFTAGSREELERIHGEWRD
                     RGLHILLDPVEKVFGYTFVAADPDGHRLRVIFNPAEQGSGS"
     gene            5777..>6205
                     /locus_tag="VFQ54_13800"
     CDS             5777..>6205
                     /locus_tag="VFQ54_13800"
                     /inference="COORDINATES: protein motif:HMM:NF002616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEU0166115.1"
                     /translation="MTEQVARRNWLAVASADHVARGVREGFMQVSHGKLAPLRRIGAD
                     DLIVYYSPSTEMGVKDGLQSFTAIGRVRPGDPYQGEMSPDVAAFRRDVEWFPSRSAAI
                     RPLLAALAITSGHANWGYRLRFGLQAIGDEDLRLIATVMGV"
BASE COUNT         1060 a         1912 c         2153 g         1080 t
ORIGIN      
        1 gcgggatctc gtcggtcagg ccgtggatct cgagcgcact gagcagacag atcgtcgcct
       61 ccggacgccg cagaacgaac gtcgccagct gcttgacgtg atcgtcgtca acatctgccc
      121 gggcaaatac cccgtcgccc atcgtttcga gaccgcccgc gtccctccag tcgtagatcc
      181 gtcgcttgga gatacccaga tcgagagctt gttctagatt gaaaacgatc ggaagatcct
      241 tgatggtgtc catagtcgtg ttcctcaacg tatacagacc tatgatagct gtatacgttg
      301 aggaacacaa gtgagaggcg ctagaccatg tcgaggtggc cctgaaggcg ggcgtagcga
      361 tccagccggc ggaaggaacg tgcgccgagg atcggcagca cgatcgcgaa gccgagcaat
      421 ggccacaagt cgccaccgag gtcggcgatc gacgcgttct tcgtcatcgc atcgacgagg
      481 gcctgcatgc cgtagccgaa cggcaggcag cgggcgggga tgcgcaacca gagcggcatc
      541 tccgagatcg gaaacatcgt tcccgagagc ggcatcagga tcgggaagac catgttcgag
      601 atctgattcg cgttccggac cgcggtgccg agcgcgctca gcatcatccc gattccccac
      661 atgcagacga tcaggagcac gagcgtcagc agtagtgccg ggacgttgac ggagaggggt
      721 gtcgcaaaga tcgcgacgcc gagtccaacc gagacgacgg cgatcgggat cgcctcgagt
      781 gcggccgaga gctgaaagcc aagcagccag gagatccgcg gcgccgggga aatgaagagc
      841 ccgccgagtg tgccgcggga gcgttcgcct tcgaggttct cgcctccact gaagagcatc
      901 gcgaagatca atgccgttcc ggttccggcg acgacggcgt agttccgcag gtcgtgatta
      961 tcccggtaga tcagcgcgac gaggacgagt tcgaagaagg ggagcccgag aaaggtcagg
     1021 acgtagaagg gcagggtcag cgcctggatc ttgatccgga tgatattgac cgcggtggcg
     1081 atggtgatcc gcagcgggcc gagaacggct gcggtccgcg tcggcacgat cccggtggtg
     1141 gatgtggatg tcgtcatcat ctcgtcgtcg ctcccgtccg atctagagga tgtcgagcgt
     1201 gccgctgcgc cgaatcgcgc ggtcggcctt gcccagcgcc cagatgccga tcacgaggaa
     1261 gatcgccgat gtccccagcg ccgccagcag cggattgcgg aggtcgccga gtgactcgcc
     1321 cgagagcatc gccccgcgca gcgcatcgac ggcatgcgag agcggaatca cgtctccgac
     1381 ccgccgcagc cagcccggca ggaccgagcg tggaacgtag aaaccggcga gcaggtagat
     1441 cgggagctgg aggacattcg agagcgagtt cgagttccgc gagagaatga agagtccccc
     1501 gaacgccatg ccgatgcaga ccgtgcttac gtagacagtg agcagcgcga cgagcgccgc
     1561 gagtgggtca tcaatggtga agcggccgcc ggcgagcagc ccgaccaccg tgcagctgac
     1621 gatcgagggg agaccgatca gcaggctccc gagggtgtag ccgatcacga cggccgccgt
     1681 gcttgccggc gccgagatga tggcgctgat cgtccccaga ttgcgctctg cctggagcgc
     1741 gttgccgcta ccccagaccg tggaactcca cgcctctgtc atgaagatgc cgatgatcag
     1801 gaagccgatc acctggctgt ccgggaccga ggtctgcccg gagatgttgt aggcgaggac
     1861 gaagccaccg agtgagagaa cgggcgcgag cgggatccgg atcagctgga tgggattcga
     1921 ggcgaagcgg agcgtgtaga cgcgggccgt cgccgcgatc acgcgcgggt tcgtccggag
     1981 caggctggtg cgcgcaggct cgcgcatcgc ggcgggaagc gcgctcattc ctggcctccc
     2041 gatgcgccgg cgcccatctg ctcgaactcg cggccggtgg cgtgcatgaa ggcgtcttcc
     2101 aatgaaggtt cgagaatctg gatgccccgg accggtgcgc ttgcctcgcg caggaccgcc
     2161 agcgcggtat cgagcgcgct cgtcgtctcc tcgcagcgga gggtcaggac cggcgcgcca
     2221 tcctccgcga gttcgtggcg gagatcgctg acgaccggaa gtcgccggag gctcgcctcg
     2281 accgagtcgc gccggccgtc gagggacgat gcgagcgtga tgatcacctg gcgatcgacg
     2341 tcggcttccg ccttgagcgt cgccggcgct ccctgggcga cgatcagtcc gcgatcgacg
     2401 atcgcgatct cgcggcagag gatgtccgcc tcgtgcatgt cgtgcgtggt gaggaggatg
     2461 gtgtgatcgg ggatcagact ggcgatcagc tcgcgaacat cgatcgcggc ggcggggtcg
     2521 agaccgatcg tcggctcgtc gaggatgatc acggcggggg aatggagcag cgtcttcgcg
     2581 atgtgaagcc gctgcttcat cccgcgcgag tagctctcga cgcgttccga ggcgcgatcc
     2641 agcagccgta cccgctcgag cagcatcgac gcgcgggatt tgatcgcggc gtcatccatg
     2701 ccgtagaggc gaccgaagta ccggagattc tcccagccgg agagctggtt gtagagcccg
     2761 cgatctcccc cgaagagcac cccgagctgc tggcggacgc gaagctcgtc ccgcgtgatc
     2821 tcgaacccgt tgatgtgggc ggtgccgctg gtgggaacga gcagcgtcga gagcatcttg
     2881 atcgtcgtcg tcttgcccgc gccgttgggc ccgagcatcc cgaagatcgt tccctgggga
     2941 acgtggaagc tgatcccgcg aacggcctcg acgagatcgc cggagcggaa ccaaccgctc
     3001 cgcttccgaa acgaacgccg gagatcggtg gcgatgatgg gtgaatccac gcttgcggat
     3061 ggcgtggtgg tgagcgccgc gggagtcgac atcttggccc ctcagacctt cggacgtgga
     3121 tggcatctga ccggcgtcga atgtggctga ccgcgggagt gtatcgtgcg gcttgcggct
     3181 cacattcggc gctgatctga tcctacgatc gggcgccgca ccgcgtggtc cgcagttgtg
     3241 cgtagttggg ggacgtaatc ctcgccggcg gtggggacgt aggcatccca cgagccagcg
     3301 cacgctgacc ggggcgctga tggctgccgc cctgccttca tatgcagact cgaacatccg
     3361 tacatggggc ttgcggtggc gactatggcg tttcggtcga ttggagcgtt acggatccct
     3421 ggactccttc ggtgctgctg gcatcatgag cgcgacgtat ccacggacac cgtcgcggcg
     3481 gtcggtgaag ggtcgtgggg cgcgatgcga aagcgggaag ggctcgcgga ccggacctat
     3541 gggaatgcgt aatcctggtt ggggaccccg cgatcgggat cgatacctgc atccaggtgc
     3601 atactgcacc gcgaaaggat gcgctcatgt cccgtgccga acgcctgctc cagctgatgc
     3661 aactgctccg tctctaccgg taccccgtga ccggtcagcg gctggcggac gagcttgggg
     3721 tcagtgtgcg gacgctttac cgcgatatcg ccacgctgca gtaccagggt gcacagatcg
     3781 acggggaacc gggtctcggc tacgtgctga gacccgggtt catgctgccg ccgatgatgt
     3841 tctccgagga ggagatcgag gcgctggtgc ttggctcccg ctgggtcgcg agccgggccg
     3901 acagcgagct ctcgctcgcc gcgcggaacg ccctcgccaa gatctcggca gtcctccccg
     3961 ccgacctgcg ggaaagtctc gacgcgtcgt cgctcatcgt gccgccgggc gacttcgtgg
     4021 cggctggcga cttcgtggcg gctggcgatc gcgaactgcg cagtatccgg gaggcgatcc
     4081 gcacggagcg tcggctccgc atcacctacg ccgatttccg cggcgaaacg accgagcgcg
     4141 tcgtctggcc cttctcgctc gccttcttcg atcgcgtgcg ggtcgtcagc gcctggtgcg
     4201 agttgcgcaa cgacttccgc gccttccgga cggacaggat cgagcatctg gcgattctgg
     4261 gcgatcgcta tccacgccgg cgccaggcgc tcctgcggga atggcgccag cgcgagggga
     4321 tctccgatcc cagtcggcct gccgcaccgg cgggaagacc tgtctcgact cctgacaaaa
     4381 actgacagtg cgcgctccta gactggaatc atggatcggg gatgtgagac gcgaaacggt
     4441 cgcccatctc acgcaactca ccggatcctg gagcgatcgt tcatggaacc gaagatgttc
     4501 atgctgtatg tgtccgacat ggatgccagt gtcgccctct atcacggggt gttgggaatt
     4561 gagccggatc gaccgactcc ggcctttgcc tcgttcctgc tttcgagcgg cctgcgattc
     4621 gacctcgtgc ttggaacggc cgtcgatccc gcgccgaccg cggggggcgg tgccatcgag
     4681 acctgtttcc tggtcgattc ccggggcgat gtcgagcgcg tccacgatct gtggcgtgcg
     4741 cgtggcctgc cgatcgcact cgcaccgcag gacaagccgt tcggatacac cctcgttggg
     4801 gtcgatccgg atggccatcg cctgcgggtg atcggtccgt ccgtcgaccc gtcgtaagtc
     4861 tcgatcgcca ttgatcgccc tggcgacgcc ggggccgtgc cgccctgtcc accaacgagg
     4921 agtgacctgt gatggaaccg aatttcacga ttctctacgt cggcgacatg ccgaagagcg
     4981 ccgccttcta cgatgccctg atccagcggg agccggtcga ggcatcggcc aattttcgag
     5041 tgtacgtcct gtcgaccggt gcgcgtgtcg cgctctggca gcgggacgat gtccagccgg
     5101 cagccacggc gccgggggga gtggagttct gccttgccgt gacgtcgccc gccgatgtcg
     5161 acgctgttgc cgccacgtgg cgcgagcggg gaattccgat cgcccaggat ccggtgctgc
     5221 tggagttcgg ctacagcttc gttggccttg atcctgatgg acatcgcttg cgtgtccatg
     5281 cgctgtcttc cgatccggcg gggaccgcgg aggcgtaatc gagatcgaac cggggcgatc
     5341 tccccgccat gagcggagat cgcatcccgg tcgagacgga aaggcaggta gcccggatgg
     5401 aaccccggta tttcgtactg cacgtcgcca gcatcgagac gagtatcgct ctctacacgg
     5461 atctgcttgg caccacgccg cagcggccgt cgcccacctt cgcggcgttc ccgctgggga
     5521 gcggcgtcat gcttgagctc tggcagcgct ccgggatcga accggcttcc gtggcgccgg
     5581 gaggagcggt cgatatcacc ttcacagccg gaagccgtga ggaactggag cggatccatg
     5641 gcgagtggcg cgatcgaggg ctgcatatcc tgctcgatcc ggtggagaag gtcttcggct
     5701 acacgttcgt cgccgccgac ccggatgggc atcgcctgcg tgtgatcttc aatccggccg
     5761 agcagggaag tgggtcatga ccgagcaggt ggcgcggcgc aactggctgg cggtggcctc
     5821 ggcggatcac gtggcgcggg gagtccgtga gggattcatg caggtgagtc atggtaaact
     5881 cgcgcccctg cgccgcatcg gcgccgatga cctcatcgtg tactactccc cgagcacgga
     5941 gatgggcgtg aaggacggcc tgcagtcgtt caccgcgatc ggccgggtgc gacccgggga
     6001 tccgtaccag ggcgagatga gtcccgacgt cgcggcattc cgtcgtgacg tggagtggtt
     6061 cccgtcccgt tcggcggcga ttcggccgtt gctcgcggcc ctcgccatca cgtcggggca
     6121 tgcgaactgg ggataccggc tgcggttcgg cttgcaggcg attggtgacg aggatctccg
     6181 gctcatcgcg acggtgatgg gcgtg
//
LOCUS       DASTMD010000930         3725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_1606763, whole genome shotgun sequence.
ACCESSION   DASTMD010000930 DASTMD010000000
VERSION     DASTMD010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606763"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFQ54_13805"
     CDS             <1..769
                     /locus_tag="VFQ54_13805"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEU0166116.1"
                     /translation="RAARYLDKSETVDFAELHAFIGPDFVVTVRHGDGSQLSGVRRRL
                     EGDPDILRMGPEAILYAILDRVVDDYGPVVAGLENDIDEIETEVFGGNAAVVRRTYEL
                     AREVIEFQRATKPLVGIVSALIVGADKHNVPIELQQYLRDVQDHAIHVQEQVAGFREL
                     LQNVLSVNLAVVGLQQNEDVKAMTEASLAQNDEVKRISAWAAILFGPTLISSVYGMNF
                     DNMPELHWKYGYPFSLGLMLLASYFIYWLVRRRGWLN"
     gene            1042..2508
                     /locus_tag="VFQ54_13810"
     CDS             1042..2508
                     /locus_tag="VFQ54_13810"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEU0166117.1"
                     /translation="MFSGGQDADGRSALIPRTGATRRRLAALVLLTIGLFLSFLPTSS
                     SAQTAPPGRIDLVTINGTITDVTHDYVKRAFDKAKGDGSQAILIELSSGGGNADSSDD
                     IITLISSSTIPVIVYVPSGDTIAGSAVTILASAQIAAMAPDASIGNASRVAAGDSKTS
                     SDQQTEQELAQFSDERGRATQWSTSATQDGYRVSGMQALSEKIIDLTAPDRATLLTDL
                     NGKTVRGGTGATVTLTTSGGGAVSETNKILMTTLERFRAFVTSPSVAYVLLCFGVLGI
                     FMELASPGGFMAGTIGLICSIAAIYAFSQLPINWAGIGLMFLAFLLLGIDLFVSSFGI
                     LTVGGLAAFIAGSYIVIDTDIVGYDHVSRPVIWTGTVCVIIFAAFIGYTGLGTLKKKP
                     VTGRKSMIGEVGVVREALDPRGMIFIYGELWQAQIVGLDAGVEIPAGEHVEVVGINGL
                     LLDVQPTTASITRSNDIGNEPRNESVIPVRDGVEGSHP"
     gene            2635..3588
                     /locus_tag="VFQ54_13815"
     CDS             2635..3588
                     /locus_tag="VFQ54_13815"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEU0166118.1"
                     /translation="MDVVVGLIIVVIIFVIIVLFASIKVVQEYERGVVFRLGRLAGAR
                     GPGLIILLPFIERMQKVDLRTVTMDIPAQEVITQDNVTIRVNAVAYFRVVDPNVAVVN
                     VADYIRATSQIAQTTLRSVLGQSALDDMLAEREKINQQLAQIIDGQTEPWGVKVSIVE
                     VKDVELPQSMQRAMARQAEAEREKRAKIIHAQGEFQSSQQLAAAAEVISQNPATIQLR
                     YLQTLNEIGAENSTTIVFPLPIDMLAAFMGSGGHSAPVTPSDPTPIRPAVPPTPTPMP
                     VVEPAEDVDPSELPPRPPRGGTPRPELKGMPAPHRGPTDNA"
BASE COUNT          691 a         1217 c         1165 g          652 t
ORIGIN      
        1 gcgggcggcg cgctatctcg acaagtcgga aacggtggac ttcgccgaac tccacgcctt
       61 catcggtccc gacttcgtcg tcacggtgcg ccacggtgac ggatcgcagc tcagtggcgt
      121 gcgtcgccgt ctcgaaggtg atcccgatat cctccgcatg ggaccggagg cgatcctcta
      181 cgcgattctc gatcgtgtcg tcgacgacta cggaccggtg gtcgccggtc tcgagaacga
      241 catcgacgag atcgaaaccg aagtcttcgg cgggaatgcc gccgtcgtcc gccgcaccta
      301 cgagcttgcg cgtgaggtga tcgagttcca gcgggcgacg aagccgctgg tcgggatcgt
      361 cagcgcgttg atcgtgggcg cggacaagca caacgtcccg atcgagttgc agcaatatct
      421 gcgggacgtg caggaccatg cgatccacgt ccaggagcag gtcgccggtt tccgcgaact
      481 cttgcagaac gtactcagcg tgaacctcgc cgtcgtcgga ctccagcaga acgaggatgt
      541 gaaggcgatg acggaagcgt cgctcgccca gaacgacgag gtgaagcgaa tctccgcctg
      601 ggcggcgatc ctcttcggtc cgacgctgat ttcgtcggtc tacggcatga acttcgacaa
      661 catgccggaa ttgcactgga agtacggcta cccgttctcg ctcggactga tgctgctggc
      721 gtcctatttc atctactggc tcgtccgtcg tcgcggttgg ctcaactaat ccctcgcgag
      781 gccccacaca acacgtcctc ccgagcttgt cgagggatca gccggcatcc acgtgctcct
      841 taccgcgccg cgtccctccc ggcatcgggc gagcaccagg cacgcccctg cgggatcgct
      901 cctggcagca ccgatccgga acggcgacaa gcgccgtggg gacgggaatg gaggggcgca
      961 acgcgttcgg cgatgccggg gtcgggtaaa gagggtgcct cccctgcgta taattgacga
     1021 agcaaggagg tcccctcgac catgttcagc ggtggccagg atgcggatgg cagatcggcg
     1081 ctcattccac gaaccggcgc aacccgccgg cgcctcgcag cactcgttct cctgacgatc
     1141 gggctctttc tttccttcct ccccacgtcg tcgtccgcgc agaccgctcc gccgggcaga
     1201 atcgatctcg tcaccatcaa tgggacgatc accgacgtca cgcatgatta cgtcaagcga
     1261 gcgttcgaca aggcgaaggg cgatggctcc caggccatcc tgatcgaact ctcgagcggc
     1321 ggcggcaacg ccgattcttc cgacgacatc atcaccctga tctcgtcgag tacgatcccg
     1381 gtcatcgtct acgttccctc cggcgacacg atcgccggct cggcggtgac catcctcgcc
     1441 tccgcccaga tcgcggcgat ggcgccggat gcgtcgatcg ggaatgcatc ccgcgtggcg
     1501 gcgggcgatt cgaagacatc gtccgaccag cagacggagc aggaactggc gcagttctcg
     1561 gacgagcgcg ggcgggcgac gcaatggtcg accagcgcga cccaggacgg gtaccgggtc
     1621 tccgggatgc aggcgctgag cgagaagatc atcgatctca cggccccgga tcgcgccacg
     1681 ctgctcacgg atctcaacgg gaagacggtc cgcggcggca ccggtgcaac cgtcacgctg
     1741 acgacctcgg gcggaggcgc ggtctccgag acgaacaaga tcttgatgac gacgctcgaa
     1801 cgcttccggg ccttcgtgac ctcaccgtcc gtggcgtacg tattgctctg cttcggtgtg
     1861 ctcgggatct tcatggaact ggcgagcccg ggtggattca tggccgggac gatcgggctg
     1921 atctgctcga tcgccgcgat ctacgccttc agtcagctgc cgatcaactg ggcgggcatc
     1981 gggctgatgt ttctcgcctt cctgctcctc gggatcgatc tcttcgtgtc gtccttcgga
     2041 atcctcaccg tgggcggact cgcggccttc atcgccggtt cctatatcgt gatcgatacc
     2101 gatatcgttg gctacgacca tgtttcgaga ccggtgatct ggacggggac ggtctgcgtg
     2161 atcatcttcg ccgcctttat cggctacaca ggacttggta ccttgaagaa gaagccagtt
     2221 accggacgca agtcgatgat cggcgaagtc ggtgtcgtcc gcgaggcgct cgatccccgc
     2281 ggcatgatct tcatctacgg cgaactctgg caggcacaga tcgtcggact cgacgccggc
     2341 gtggagattc ccgccggcga gcatgtcgag gtcgtcggga tcaacgggct gctgctcgat
     2401 gtccagccaa cgacggcatc gatcactcgc tccaacgata tcgggaacga accacgcaac
     2461 gagagcgtca ttcccgtacg ggacggcgtc gaaggctcgc acccatagca atccgggcgt
     2521 gaaaacgagc gccgcccttc accacgcacc caacggcgga cggttccgaa acgccggaac
     2581 cgcggccacg gagtcagcct cgcccttccg ggcgagaggg agccatcaag aacgatggat
     2641 gttgttgtag gactcattat tgtcgtcatc atctttgtca tcatcgtgct ctttgcctcg
     2701 atcaaggtcg tgcaggagta cgagcgtggt gtcgtcttcc ggcttggacg tctcgccggc
     2761 gcccgcggtc ccggcctgat catcctgctc ccgttcatcg agcgcatgca gaaggtcgat
     2821 ctccggacgg taacgatgga cattccggca caggaagtca tcacccagga caatgtcacg
     2881 atccgcgtga acgccgtcgc atacttccgc gtcgtcgatc cgaacgtggc ggtggtgaat
     2941 gtcgccgact acatccgggc gacctcacag atcgcgcaga cgacactccg gagcgtgctc
     3001 ggccagtccg ccctcgacga catgctcgcc gagcgggaga agatcaacca gcaactggcg
     3061 cagatcatcg acggccagac cgagccgtgg ggcgtcaagg tcagcatcgt cgaggtgaag
     3121 gacgtcgaat tgccgcagtc gatgcagcgc gcgatggctc gccaggccga ggcggaacgc
     3181 gagaagcggg cgaagatcat tcacgcccag ggtgaattcc agtccagcca gcagctggcg
     3241 gccgcggcag aagtgatcag ccagaacccg gcgacgatcc agctgcgcta cctgcagacg
     3301 ctgaacgaga tcggcgccga gaacagcacg acgatcgtct tcccgctccc gatcgacatg
     3361 ctcgcggctt tcatgggcag tggcggtcac agcgcaccgg tcacgccatc cgatccgacg
     3421 ccgatccggc cggcagtgcc gccgacccca accccgatgc cggtcgtcga gccggcggaa
     3481 gacgtcgacc cgagcgaact gccaccgcga ccgccgcggg gtggcacgcc acgccctgaa
     3541 ctgaagggga tgccggcgcc gcatcgcggc ccgaccgaca acgcctgatc gcggcgggtt
     3601 caacatgatc gtcgtgaaga acccgtcgtt gtccgctatc atcacggcag aggacaatga
     3661 gcgagccgcc tggctcgctc atctgcgtgc cccgacgggc gttgcccgat cccgaaacct
     3721 ggatt
//
LOCUS       DASTMD010000931         1591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_175831, whole genome shotgun sequence.
ACCESSION   DASTMD010000931 DASTMD010000000
VERSION     DASTMD010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175831"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /gene="argG"
                     /locus_tag="VFQ54_13820"
     CDS             complement(<1..767)
                     /gene="argG"
                     /locus_tag="VFQ54_13820"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU0166119.1"
                     /translation="MANILQQLPVGEKIGIAFSGGLDTSAAIAWMRSKGAIPYAYTAN
                     LGQPDEDDYEDIPRRALEYGAEGARLIDCRSQLVAEGLTALQCGAFHITTAGIAYFNT
                     TPLGRAVTGTMLVAAMKEDDVNIWGDGSTYKGNDIERFYRYGLLVNPALRIYKPWLDD
                     AFIDELGGRAEMSEFLTKGGFNYRMSAEKAYSTDSNILGATHEAKDLEQLDSNAYIVN
                     PIMGVPFWREDQPIAYEEVTIAFENGLPVAINGHAFA"
     gene            complement(903..>1591)
                     /locus_tag="VFQ54_13825"
     CDS             complement(903..>1591)
                     /locus_tag="VFQ54_13825"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU0166120.1"
                     /translation="PPERVPRAQKEIIGVANQLGLPVITATQMLESMINQPVATRAET
                     SDVANAVWDGTDAVMLSAESAVGKFPIEAVQTMDRIIKEVEKDGPVRSAASQQPPKLE
                     DPGAKFSDAVARAAYALSDQTPAESLVVFSKSGAAVRHIAKYRPTPPIIAAVDTEETA
                     RRLSLVWGVTSVVVPVEYDPDVLFRKTGRLIVEQGLAEEGDYALIVGSLPMTNVSGRT
                     NMVHFRKLGE"
BASE COUNT          261 a          512 c          516 g          302 t
ORIGIN      
        1 tcggcgaacg cgtgaccgtt gatcgccacc ggcaacccgt tctcgaacgc gatcgtcacc
       61 tcttcatagg cgatcggctg atcctcccgc cagaatggca cacccatgat cgggttgacg
      121 atataggcgt tgctgtccag ctgctcgaga tcctttgcct cgtgcgtcgc accgaggatg
      181 ttggaatccg tcgagtacgc cttctcggcc gacatccggt aattgaagcc gcccttcgtc
      241 aggaactcgg acatctcggc gcgaccaccg agttcgtcga tgaaggcgtc gtcgagccat
      301 ggcttgtaga tccgcagcgc gggattcacg agcagcccgt agcggtagaa acgctcgatg
      361 tcgttgccct tgtaggtgct gccgtccccc cagatgttga cgtcgtcttc cttcatcgcg
      421 gcgacgagca tcgtcccggt caccgcgcgt ccgagcgggg tcgtgttgaa ataggcgatg
      481 cccgcggtcg tgatgtggaa cgccccgcac tggagtgccg tcaatccttc ggcgacgagt
      541 tgcgaccggc aatcgatcag gcgtgcgcct tcagcaccgt actcgagggc gcgacgaggg
      601 atgtcctcgt aatcgtcctc gtcgggctgg ccgagattcg cggtgtacgc gtaggggatc
      661 gcgcccttgg aacgcatcca ggcgatcgcg gcgctggtat cgaggcctcc cgagaacgcg
      721 atgccgatct tctcgccaac gggcaactgc tgaaggatgt tcgccatagt ctttcctgaa
      781 tccaatcgaa taccgaccaa acgtcccgca catagtcgaa tccacgacct gccgcgtcaa
      841 gcgtggcaaa tcgtggattc gtcgttcgcg atcccgagcc gcgagcggcc ggtgaagatc
      901 gcctattcgc cgagtttgcg gaagtgcacc atgttcgtgc gaccggacac gttcgtcatt
      961 ggcagcgagc cgacgatcag cgcgtagtcg ccctcttcgg cgagcccctg ctccacgatc
     1021 aggcgacccg tcttgcggaa gaggacgtcc ggatcgtatt cgaccggcac gacgacactc
     1081 gtgacgcccc agacgaggct caggcgtcgc gccgtctcct cggtatcgac cgcggcgatg
     1141 atcggtggcg tcggtcggta cttcgcgatg tggcggacgg cggcgccgct cttcgagaag
     1201 acgaccagcg attccgccgg cgtctgatcc gagagcgcgt aggccgcacg ggcgacggca
     1261 tccgagaact tcgcgcccgg gtcctcgagc ttcggcggct gctgcgaggc cgcggagcgg
     1321 accgggccgt ccttctcgac ttccttgatg atgcgatcca tcgtctgcac ggcctcgatc
     1381 gggaacttgc cgaccgcgga ctcggcggag agcatcacgg cgtcggtccc gtcccagacg
     1441 gcgttcgcga cgtcgctggt ctcggcgcgg gtggcgaccg gctggttgat catcgactcg
     1501 agcatctggg tcgccgtgat cacggggagg ccgagctggt tggcgacgcc gatgatctcc
     1561 ttctgggcgc ggggaacgcg ctccggcggc a
//
LOCUS       DASTMD010000932         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_176025, whole genome shotgun sequence.
ACCESSION   DASTMD010000932 DASTMD010000000
VERSION     DASTMD010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176025"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFQ54_13830"
     CDS             <1..524
                     /locus_tag="VFQ54_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166121.1"
                     /translation="TISTGDGGDVPDSAQVCVGDVCQTVGASVSSAAVSPATLTFGDL
                     APGIYDVTVTNAAPYQNSAGSIAITAGETTTSDIVLQVAAVTVAPTRPGNATPITTVT
                     VAPTNPSSGGDGGGGSAPSNPGAPSGGTTVRALPNTGSGSSGSNGSLLLLLSALAITG
                     LGGWAAIRRRRNP"
     gene            complement(991..2316)
                     /locus_tag="VFQ54_13835"
     CDS             complement(991..2316)
                     /locus_tag="VFQ54_13835"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU0166122.1"
                     /translation="MDPTARRALAWLRMLLFFLGVGGLLLSPRAIVAAGSPSANNDAY
                     ADDFNTTMEIFVPGVLANDYDPDGSPLHTSPVQDATYGTLTLYPDGSFTYTPAPGFFG
                     QDSFTYVAITNDSRISNIATVTIYVSSPPPTDIPTLVPTDTPTLVPTATPSDTPTSIP
                     TETLVPTDTATAVPTSTATNTPVPTDTATTIPTDTPTVVPTNTSTAVPTTTVAPTSTT
                     VSATLSPSMTNVAATDVPTGTNVPPTEVPTGTAGTTGSVALTLTTDDGGPVPDGAIVC
                     VGNQCQTVGAEVSSAAVSATTLTFASLDPGTYLLTVTNAAPYADAAGSVTVTSDGTAQ
                     VTVTLETVAAPTTVATQPGNATPVTTVTVVPTQGSGGGVVIPTSPPTSGVGGLTPSNP
                     NAPTGGMTVKALPNTGSGQGASSTSLVMLLLAVLAVGVAGAFAWRRRTR"
     gene            complement(2542..2606)
                     /locus_tag="VFQ54_13840"
     tRNA            complement(2542..2606)
                     /locus_tag="VFQ54_13840"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(2816..3052)
                     /locus_tag="VFQ54_13845"
     CDS             complement(2816..3052)
                     /locus_tag="VFQ54_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0166123.1"
                     /translation="MLIARQWVWSSPTHDAAVADAIETTGAVKPSVTTVTATTTPAIA
                     VPKPEPTAPVLPVETPKPEPVPVTTDQKRRCRWT"
     gene            complement(3106..>3785)
                     /locus_tag="VFQ54_13850"
     CDS             complement(3106..>3785)
                     /locus_tag="VFQ54_13850"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU0166124.1"
                     /translation="LTAPKALSTVSTANWDLSVWEGAALSETNVRQASNTKSKEMKTL
                     EFGDPVVVVEWVAGEEVYEGANIWGKLKDGTFVYARNVGRHAPVAATPIPGDAPTYGK
                     WIDINLTQQLMVAYDGTKVARVSVMTAGMPGHETPTGYFAINSRVANETMKAGTPGAD
                     GYYNLANCLFTQYFTEYGHAIHFAWWRTPETIGRPGSHGCINLLLDESQFYWDWATIG
                     TPVIVHK"
BASE COUNT          660 a         1204 c         1211 g          710 t
ORIGIN      
        1 tcaccatttc gaccggggat ggaggtgatg ttccagattc ggcccaggtc tgcgtcggtg
       61 acgtctgcca gacggtcggg gcatccgtga gttccgccgc cgtctcgccg gcaacactga
      121 cgttcggtga cctcgccccc ggcatctacg acgtcacggt gacgaatgcc gccccgtatc
      181 agaactcggc tggaagcatt gcgatcaccg ccggcgaaac gacgaccagc gacatcgtgc
      241 tccaggtggc ggcggtcacg gtcgcgccga cgcgaccggg gaacgccacc ccgattacga
      301 cggtgaccgt tgccccgacg aatccatcct ccggcggtga cggtggcggt ggtagcgcac
      361 catcgaaccc gggtgccccg tccggcggca cgacggtgag ggcactgccg aatacggggt
      421 ccggctcgtc cggttcgaat ggctcgctgc tgctcctgct atcggccctg gcaatcaccg
      481 ggttgggtgg ctgggcggca atacggagac gccggaaccc ctgacagctg catcgtcccg
      541 cggaccgagc ccgtcagcga tgcgagatca ctgacgggct cggtcctggc ggcgcagcgt
      601 ttgcgtccga agccgggcga atagcgcgga gaaccgatgc ggcaggaggt tgccgcgatc
      661 acgctatgcg tggacgatca ccggcgttcc gatacttgtc ctgaaatgac catcatgtcg
      721 cccgcgagta tcaaacgcgc aaacttcggc agaattgctt tccttatttg ttcggtgact
      781 atatgttcgg tgactcccct aatcacgatt tgccaaacgc ccgtcacgga gatgctcgca
      841 ggctgcacgg tcctcatttc ggggatcgtg cggccgcgac taacatcggg cgagatgagc
      901 ctcgacgagc agtccgtatt ctccagcgat aacacgaaag agcccggacg tttcgagcgt
      961 ccgggctcgc gtcttgcctt cttcgtcagg ctatcgcgtg cgtcgtctcc aggcgaacgc
     1021 acccgccaca ccgacggcga gcaccgccag cagcagcatg accagactcg tcgaggaagc
     1081 tccctgaccc gatcccgtgt tcggcagcgc ctttacggtc atgccgccgg tcggggcatt
     1141 cgggttcgag ggcgtgagcc cgccaacgcc gctcgttggc ggactcgtgg gaatcaccac
     1201 gcctccacct gagccctggg ttggaaccac ggtcaccgtc gtgaccggtg tcgcgttccc
     1261 cggctgggtc gccaccgtgg tcggagcagc caccgtctca agcgtgaccg taacttgcgc
     1321 cgtcccgtct gacgtgaccg tgacactacc tgcggcatcg gcatacggcg ccgcgttggt
     1381 cacggtcagt agataggtcc cgggatcaag gctggcgaag gtcagcgttg tggcggacac
     1441 ggcagcggag ctcacctcgg caccgaccgt ctggcactga tttccgacgc aaacgatcgc
     1501 cccatccgga accgggcccc cgtcatccgt ggtcagcgtc agggcgaccg agccagtcgt
     1561 ccccgcggtt ccggtcggga cctcagtggg cggcacgttg gttccggtcg ggacgtcggt
     1621 agccgccaca ttggtcatgc tcgggctcag agtcgccgag acggtcgtgc tcgtcggcgc
     1681 gacggtcgtg gtcggcactg ccgtcgacgt attcgtcgga acgacagtcg gggtatcggt
     1741 tggaatcgtg gttgcggtat cggttgggac gggcgtattc gtcgccgtgc tcgtgggaac
     1801 agcggtcgcg gtatccgtcg ggaccaatgt ctccgtcggt atgctggtcg gtgtatcgct
     1861 cggtgtcgcg gtgggaacca acgttggggt atcggtcggc accagggtcg ggatatcggt
     1921 tggcggagga gacgacacgt agatcgtgac cgtggcaatg ttcgaaatcc ggctgtcgtt
     1981 cgtgatggcc acgtacgtga agctatcctg cccgaaaaag ccaggtgccg gtgtgtaggt
     2041 gaacgagccg tcggggtaga gtgtcaacgt cccgtacgtg gcatcctgga caggcgacgt
     2101 gtgcaatggg gagccatcgg gatcgtagtc atttgccaac acgccgggca caaagatttc
     2161 catcgtcgtg ttgaagtcgt cagcgtaggc atcattgttt gcgctgggtg accctgccgc
     2221 cacgatcgcc cgaggactca gcagaagtcc cccgacgcca agaaaaaaga gcagcattcg
     2281 aagccacgcg agcgcgcggc gagccgttgg atccatgagc gacagtctcc ctcagatgcc
     2341 gtcaatggat tcgtcgacac ggttgtcacg gtggtccagg gcacttggtc acgatacgcc
     2401 aactttgcca tatagacaag gcatgcacta tacgagaatg gccctcgtgg attatgcaga
     2461 acatgattgt ataaaatggg ccgccgactg ttacggatgc tgcataggaa acggaacgct
     2521 gactcaaagc ggagagcccg gagcccccag aggattcgaa cctctaccga atgcttgcac
     2581 tcgctctgcc gttgagctag agaggcgtct gggacgccgc accccaccgg gcgtcgtatc
     2641 agctccaccg cctgagatcc gtagccgaac ccggaatcgc accggggacc tccggagtat
     2701 gaacccggcg ctccgcatgt tgagctattc cacagtaacg tggatatcaa atatgaagcg
     2761 ttacgccact tggtgacgtg aacgacctga ccgtcggagg cgaccaacgg cggcatcaag
     2821 tccacctgca acggcgtttc tggtcggtcg tgactggcac gggttcgggc ttcggtgtct
     2881 cgaccggaag cacgggcgcg gtcggctccg gcttcggtac ggcgatcgcc ggcgtcgtgg
     2941 tggccgtgac ggtcgtcacg ctcggcttga ccgcgcccgt cgtctcgatc gcgtccgcca
     3001 ccgcggcgtc atgggtcggc gaggaccaca cccactgccg cgcgatcagc accagcaggg
     3061 tcgggccgtg gaactcctgc agccagcaac cgcccgtcta ccgccctact tgtggacgat
     3121 caccggcgtt ccgatcgtcg cccaatccca gtagaactgg gactcgtcga gcagcaggtt
     3181 gatgcagccg tggctacccg gacgcccgat cgtctccggc gtccgccacc acgcgaagtg
     3241 gatggcgtgc ccatactccg tgaagtattg ggtgaagagg cagttggcga ggttgtagta
     3301 gccgtccgcg cccggcgtcc ccgccttcat cgtctcgttc gccacccggc tattgatcgc
     3361 gaagtacccg gtcggggtct cgtggccggg catcccggcc gtcatcacgc tcacccgcgc
     3421 gaccttcgtg ccgtcgtagg cgaccatcag ctgttgggtc agattgatat cgatccactt
     3481 cccgtaggtg ggagcatcgc cggggatcgg cgtcgccgcg accggcgcgt gtcgaccgac
     3541 gtttcgcgcg tagacgaatg tgccgtcctt cagcttgccc cagatgttcg cgccctcata
     3601 gacttcctcg ccggcaaccc actccaccac gacgaccggg tcgccaaact cgagcgtctt
     3661 catctccttc gacttcgtgt tggatgcctg acgaacattc gtctcgctga gcgcggcgcc
     3721 ctcccacacg ctgagatccc aattggcggt actcacggtc gagagtgcct tcggcgccgt
     3781 caggt
//
LOCUS       DASTMD010000933         1942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U3440
            k141_267718, whole genome shotgun sequence.
ACCESSION   DASTMD010000933 DASTMD010000000
VERSION     DASTMD010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970051
            Sequence Read Archive: SRR10968246
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Pseudomonadati; Thermomicrobiota; Thermomicrobia;
            Thermomicrobiales.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:37:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,739
            CDSs (with protein)               :: 2,739
            Genes (RNA)                       :: 17
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 9 of 16
            Pseudo Genes (internal stop)      :: 8 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267718"
                     /isolate="SMAG_U3440"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /geo_loc_name="Czech Republic"
                     /lat_lon="48.66447093 N 14.70930506 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VFQ54_13855"
     CDS             complement(<1..141)
                     /locus_tag="VFQ54_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU0166125.1"
                     /translation="MAKKVFERTKPHVNIGTIGHVDHGKTTLTAAISKTLSLQGGAAF
                     RDF"
     gene            complement(244..>1942)
                     /gene="fusA"
                     /locus_tag="VFQ54_13860"
     CDS             complement(244..>1942)
                     /gene="fusA"
                     /locus_tag="VFQ54_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU0166126.1"
                     /translation="VPRICFVNKLDRTGASFVRTLEMIVDRLKAKPAPIQLPIGLEGE
                     FRGIVDLINMKAIIYTNDEGTNIQETEIPENLLAEAEAARLTLIETIAETDEDLTTRY
                     LEGGDFTPEELKAALRAATIRSELVPILCGSALKNKGVQPMLDAVVDFLPSPLDVPPV
                     VGINPNNDEEVIRPADPKAPFSALAFKIATDPHVGKLVYIRVYSGTVQSGSYVTNTTK
                     DQRERIGRLLQMHANHREEISEIAAGNIGAVIGLKTTFTGDTLSDPANPVILENIVFP
                     EPVISVSVEPKTRADQDKMGSALQRLAEEDPTFRVRTDEESGQTVIDGMGELHLAIIV
                     DRMQREFRVEANIGRPQVAYRETITIPVRAEGRHVRQSGGKGQYGHVIVEFEPQERGA
                     GYEFVDKIVGAAIPREYVQPANQGIQQALLTGGPAGFPIVDIKATIVDGSYHDVDSSE
                     MAFTIAGSLALKEAVRKGRPTILEPVMSVEVTTPEEFMGDVIGDLNSRRGQIEGMEER
                     AGAQVVRASVPLANMFGYVTDLRSMTQGRAVSSMEFHHYAPLPEALAAEFVAKNQRA"
BASE COUNT          315 a          647 c          609 g          371 t
ORIGIN      
        1 gaagtcccgg aacgccgccc cgccctgcag cgacagcgtc ttcgagatcg ccgccgtcag
       61 cgtcgtcttc ccgtggtcca cgtgcccgat cgtcccgatg ttcacatgcg gcttcgtccg
      121 ctcaaatacc ttcttcgcca ccggtcttgc tccttggtcc ccgaatctcg taccggggga
      181 gatccgccgt ttacccggcg gtcaacccgg atggcacgaa ctgccatccg ggttctgcca
      241 gcgctaggcg cgctgattct ttgccacaaa ctcggctgcg agggcttccg gcagcggcgc
      301 atagtggtgg aactccatcg aggagaccgc ccgaccctgc gtcatcgacc ggagatccgt
      361 cacgtagcca aacatgttcg ccagcgggac cgacgcacgg acgacctggg caccagcccg
      421 ctcctccatg ccttcgatct gaccgcggcg agagttcagg tcaccgatga cgtcgcccat
      481 gaactcttcc ggtgtcgtca cttcgaccga catcaccggt tcgaggatcg tcgggcgtcc
      541 cttgcggacg gcttccttca gcgccagcga accggcgatc gtgaaggcca tttccgacga
      601 gtcgacgtcg tgatacgaac cgtcgacgat cgtcgccttg atgtcgacga tcgggaaccc
      661 ggccgggccg cctgtcagca gcgcctgctg gataccctgg ttcgccggct gcacgtattc
      721 gcgcggaatc gcggcgccga cgatcttgtc gacgaactcg tagcccgcgc cgcgctcctg
      781 cggctcgaac tccacgatga cgtggccgta ctgaccctta ccaccggact ggcggacgtg
      841 acgaccctcg gcgcgaaccg ggatcgtgat cgtctcgcga tacgcgacct gcgggcgacc
      901 gatattcgcc tcgacgcgga actcgcgctg catgcggtcg acgatgatcg cgaggtgaag
      961 ctcacccata ccatcgatga ccgtctgccc actctcctcg tccgtgcgga cgcggaaggt
     1021 cggatcctct tcggccagtc gctggagcgc gctgcccatc ttgtcctggt cggcgcgcgt
     1081 cttcggctcg accgagaccg agatcaccgg ctccgggaag acgatgttct cgaggatgac
     1141 cgggttcgcc ggatccgaga gcgtgtcgcc cgtgaacgtc gtcttgagac cgatcaccgc
     1201 gccgatgttg ccggccgcga tctcggagat ctcctcgcgg tgattggcgt gcatctgcag
     1261 caggcgaccg atgcgctcgc gctggtcctt cgtcgtgttg gtgacgtagg aaccggactg
     1321 caccgtaccg gagtagacac ggatgtagac cagcttcccg acgtgcggat ccgtggcgat
     1381 cttgaaggcc agtgccgaga acggcgcctt cggatcggcc gggcggatga cttcctcgtc
     1441 gttgttcgga ttgattccga ccaccggcgg cacgtccagc ggggacggca ggaaatcgac
     1501 gacggcgtcc agcatcggct gcacgccctt gttcttgagg gcgctgccgc agaggatcgg
     1561 cacaagctca ctgcggatgg tggcggcacg cagggcggcc ttcaactctt ccggcgtgaa
     1621 gtcgccaccc tcgaggtagc gcgtcgtcag gtcctcatcg gtctcggcga tcgtctcgat
     1681 cagcgtgagg cgcgcggcct ccgcctcggc gagaagattc tccgggatct cggtttcctg
     1741 gatgttcgtg ccctcatcat tggtgtagat gatggccttc atattgatca ggtcgacgat
     1801 tccgcggaac tcgccttcga gaccgatcgg cagctggatc ggcgccggct tcgccttcag
     1861 gcggtcgaca atcatctcga gcgtgcgcac gaaggaagca ccggtgcgat cgagcttgtt
     1921 cacgaagcag atgcgcggca cg
//
